LOCUS       DACDEG010000001       138212 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.001, whole genome shotgun sequence.
ACCESSION   DACDEG010000001 DACDEG010000000
VERSION     DACDEG010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 138212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 138212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..138212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.001"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(658..1014)
                     /locus_tag="IV142_000001"
     CDS             complement(658..1014)
                     /locus_tag="IV142_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP8483692.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(1028..1438)
                     /locus_tag="IV142_000002"
     CDS             complement(1028..1438)
                     /locus_tag="IV142_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8483693.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            complement(1785..3971)
                     /locus_tag="IV142_000003"
     CDS             complement(1785..3971)
                     /locus_tag="IV142_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP8483694.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            4189..4872
                     /locus_tag="IV142_000004"
     CDS             4189..4872
                     /locus_tag="IV142_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP8483695.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(4953..5672)
                     /locus_tag="IV142_000005"
     CDS             complement(4953..5672)
                     /locus_tag="IV142_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8483696.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(5984..6190)
                     /locus_tag="IV142_000006"
     CDS             complement(5984..6190)
                     /locus_tag="IV142_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8483697.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            6435..7037
                     /locus_tag="IV142_000007"
     CDS             6435..7037
                     /locus_tag="IV142_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP8483698.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            7178..8458
                     /locus_tag="IV142_000008"
     CDS             7178..8458
                     /locus_tag="IV142_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8483699.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            8471..9199
                     /locus_tag="IV142_000009"
     CDS             8471..9199
                     /locus_tag="IV142_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8483700.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(9268..10791)
                     /locus_tag="IV142_000010"
     CDS             complement(9268..10791)
                     /locus_tag="IV142_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP8483701.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            11043..12224
                     /locus_tag="IV142_000011"
     CDS             11043..12224
                     /locus_tag="IV142_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8483702.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKCLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(12284..13912)
                     /gene="treC"
                     /locus_tag="IV142_000012"
     CDS             complement(12284..13912)
                     /gene="treC"
                     /locus_tag="IV142_000012"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP8483703.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(14309..17020)
                     /locus_tag="IV142_000013"
     CDS             complement(14309..17020)
                     /locus_tag="IV142_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP8483704.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(17030..17731)
                     /locus_tag="IV142_000014"
     CDS             complement(17030..17731)
                     /locus_tag="IV142_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8483705.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            17917..18516
                     /locus_tag="IV142_000015"
     CDS             17917..18516
                     /locus_tag="IV142_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8483706.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            18597..19610
                     /locus_tag="IV142_000016"
     CDS             18597..19610
                     /locus_tag="IV142_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8483707.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            19718..20635
                     /gene="rbsK"
                     /locus_tag="IV142_000017"
     CDS             19718..20635
                     /gene="rbsK"
                     /locus_tag="IV142_000017"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP8483708.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            20663..21058
                     /gene="rbsD"
                     /locus_tag="IV142_000018"
     CDS             20663..21058
                     /gene="rbsD"
                     /locus_tag="IV142_000018"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP8483709.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            21058..22431
                     /locus_tag="IV142_000019"
     CDS             21058..22431
                     /locus_tag="IV142_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8483710.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            22788..24905
                     /locus_tag="IV142_000020"
     CDS             22788..24905
                     /locus_tag="IV142_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8483711.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIPETTTRTVKA
                     ATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(24957..25997)
                     /locus_tag="IV142_000021"
     CDS             complement(24957..25997)
                     /locus_tag="IV142_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8483712.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(26274..28076)
                     /locus_tag="IV142_000022"
     CDS             complement(26274..28076)
                     /locus_tag="IV142_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8483713.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(28090..28842)
                     /locus_tag="IV142_000023"
     CDS             complement(28090..28842)
                     /locus_tag="IV142_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8483714.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(28851..29213)
                     /locus_tag="IV142_000024"
     CDS             complement(28851..29213)
                     /locus_tag="IV142_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8483715.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(29389..30261)
                     /locus_tag="IV142_000025"
     CDS             complement(29389..30261)
                     /locus_tag="IV142_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP8483716.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(30312..30800)
                     /locus_tag="IV142_000026"
     CDS             complement(30312..30800)
                     /locus_tag="IV142_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP8483717.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(30938..31768)
                     /locus_tag="IV142_000027"
     CDS             complement(30938..31768)
                     /locus_tag="IV142_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP8483718.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            complement(31784..32392)
                     /locus_tag="IV142_000028"
     CDS             complement(31784..32392)
                     /locus_tag="IV142_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP8483719.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(32385..32702)
                     /locus_tag="IV142_000029"
     CDS             complement(32385..32702)
                     /locus_tag="IV142_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8483720.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(32858..33586)
                     /locus_tag="IV142_000030"
     CDS             complement(32858..33586)
                     /locus_tag="IV142_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP8483721.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            33745..34626
                     /locus_tag="IV142_000031"
     CDS             33745..34626
                     /locus_tag="IV142_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8483722.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(34863..35906)
                     /locus_tag="IV142_000032"
     CDS             complement(34863..35906)
                     /locus_tag="IV142_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8483723.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(35909..36577)
                     /locus_tag="IV142_000033"
     CDS             complement(35909..36577)
                     /locus_tag="IV142_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8483724.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            36719..39055
                     /locus_tag="IV142_000034"
     CDS             36719..39055
                     /locus_tag="IV142_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8483725.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGNI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(39170..39481)
                     /locus_tag="IV142_000035"
     CDS             complement(39170..39481)
                     /locus_tag="IV142_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8483726.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            39662..41137
                     /locus_tag="IV142_000036"
     CDS             39662..41137
                     /locus_tag="IV142_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP8483727.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            41184..41768
                     /locus_tag="IV142_000037"
     CDS             41184..41768
                     /locus_tag="IV142_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP8483728.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            41770..42189
                     /locus_tag="IV142_000038"
     CDS             41770..42189
                     /locus_tag="IV142_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP8483729.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(42470..42754)
                     /locus_tag="IV142_000039"
     CDS             complement(42470..42754)
                     /locus_tag="IV142_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP8483730.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(43106..43816)
                     /locus_tag="IV142_000040"
     CDS             complement(43106..43816)
                     /locus_tag="IV142_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP8483731.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(43813..45285)
                     /locus_tag="IV142_000041"
     CDS             complement(43813..45285)
                     /locus_tag="IV142_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8483732.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            45423..45896
                     /locus_tag="IV142_000042"
     CDS             45423..45896
                     /locus_tag="IV142_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8483733.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(46049..46600)
                     /locus_tag="IV142_000043"
     CDS             complement(46049..46600)
                     /locus_tag="IV142_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8483734.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            46705..47235
                     /locus_tag="IV142_000044"
     CDS             46705..47235
                     /locus_tag="IV142_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP8483735.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(47718..48161)
                     /locus_tag="IV142_000045"
     CDS             complement(47718..48161)
                     /locus_tag="IV142_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8483736.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(48177..49337)
                     /locus_tag="IV142_000046"
     CDS             complement(48177..49337)
                     /locus_tag="IV142_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8483737.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            49844..51544
                     /locus_tag="IV142_000047"
     CDS             49844..51544
                     /locus_tag="IV142_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8483738.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(51646..52479)
                     /locus_tag="IV142_000048"
     CDS             complement(51646..52479)
                     /locus_tag="IV142_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP8483739.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(52783..54948)
                     /locus_tag="IV142_000049"
     CDS             complement(52783..54948)
                     /locus_tag="IV142_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8483740.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            55208..57502
                     /locus_tag="IV142_000050"
     CDS             55208..57502
                     /locus_tag="IV142_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP8483741.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            57495..58160
                     /gene="pgmB"
                     /locus_tag="IV142_000051"
     CDS             57495..58160
                     /gene="pgmB"
                     /locus_tag="IV142_000051"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP8483742.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            58153..59160
                     /locus_tag="IV142_000052"
     CDS             58153..59160
                     /locus_tag="IV142_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8483743.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            59182..60201
                     /locus_tag="IV142_000053"
     CDS             59182..60201
                     /locus_tag="IV142_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8483744.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(60482..61849)
                     /gene="nhaC"
                     /locus_tag="IV142_000054"
     CDS             complement(60482..61849)
                     /gene="nhaC"
                     /locus_tag="IV142_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP8483745.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(61958..63379)
                     /locus_tag="IV142_000055"
     CDS             complement(61958..63379)
                     /locus_tag="IV142_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8483746.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(63622..65499)
                     /gene="tdc"
                     /locus_tag="IV142_000056"
     CDS             complement(63622..65499)
                     /gene="tdc"
                     /locus_tag="IV142_000056"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP8483747.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(65790..67046)
                     /locus_tag="IV142_000057"
     CDS             complement(65790..67046)
                     /locus_tag="IV142_000057"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP8483748.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            67626..68927
                     /locus_tag="IV142_000058"
     CDS             67626..68927
                     /locus_tag="IV142_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP8483749.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(68996..69595)
                     /locus_tag="IV142_000059"
     CDS             complement(68996..69595)
                     /locus_tag="IV142_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8483750.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            69849..70730
                     /locus_tag="IV142_000060"
     CDS             69849..70730
                     /locus_tag="IV142_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8483751.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            70743..72353
                     /locus_tag="IV142_000061"
     CDS             70743..72353
                     /locus_tag="IV142_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP8483752.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(72430..73089)
                     /locus_tag="IV142_000062"
     CDS             complement(72430..73089)
                     /locus_tag="IV142_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8483753.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(73372..74037)
                     /locus_tag="IV142_000063"
     CDS             complement(73372..74037)
                     /locus_tag="IV142_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8483754.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(74184..74789)
                     /gene="dhaL"
                     /locus_tag="IV142_000064"
     CDS             complement(74184..74789)
                     /gene="dhaL"
                     /locus_tag="IV142_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP8483755.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(74817..75800)
                     /gene="dhaK"
                     /locus_tag="IV142_000065"
     CDS             complement(74817..75800)
                     /gene="dhaK"
                     /locus_tag="IV142_000065"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP8483756.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(75802..76197)
                     /gene="dhaM"
                     /locus_tag="IV142_000066"
     CDS             complement(75802..76197)
                     /gene="dhaM"
                     /locus_tag="IV142_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP8483757.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            76351..76671
                     /locus_tag="IV142_000067"
     CDS             76351..76671
                     /locus_tag="IV142_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP8483758.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(76783..77652)
                     /locus_tag="IV142_000068"
     CDS             complement(76783..77652)
                     /locus_tag="IV142_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8483759.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(77748..79052)
                     /locus_tag="IV142_000069"
     CDS             complement(77748..79052)
                     /locus_tag="IV142_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8483760.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(79049..79558)
                     /locus_tag="IV142_000070"
     CDS             complement(79049..79558)
                     /locus_tag="IV142_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8483761.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(79733..81868)
                     /locus_tag="IV142_000071"
     CDS             complement(79733..81868)
                     /locus_tag="IV142_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8483762.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(81951..83606)
                     /locus_tag="IV142_000072"
     CDS             complement(81951..83606)
                     /locus_tag="IV142_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8483763.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(83608..83922)
                     /locus_tag="IV142_000073"
     CDS             complement(83608..83922)
                     /locus_tag="IV142_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8483764.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            84092..84352
                     /locus_tag="IV142_000074"
     CDS             84092..84352
                     /locus_tag="IV142_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP8483765.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            84366..84680
                     /locus_tag="IV142_000075"
     CDS             84366..84680
                     /locus_tag="IV142_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP8483766.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(85410..85964)
                     /locus_tag="IV142_000076"
     CDS             complement(85410..85964)
                     /locus_tag="IV142_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP8483767.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(86090..88018)
                     /locus_tag="IV142_000077"
     CDS             complement(86090..88018)
                     /locus_tag="IV142_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP8483768.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(88324..89040)
                     /locus_tag="IV142_000078"
     CDS             complement(88324..89040)
                     /locus_tag="IV142_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP8483769.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(89041..90864)
                     /gene="glpO"
                     /locus_tag="IV142_000079"
     CDS             complement(89041..90864)
                     /gene="glpO"
                     /locus_tag="IV142_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP8483770.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(90878..92374)
                     /gene="glpK"
                     /locus_tag="IV142_000080"
     CDS             complement(90878..92374)
                     /gene="glpK"
                     /locus_tag="IV142_000080"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP8483771.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(92510..93970)
                     /locus_tag="IV142_000081"
     CDS             complement(92510..93970)
                     /locus_tag="IV142_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8483772.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(93960..95255)
                     /locus_tag="IV142_000082"
     CDS             complement(93960..95255)
                     /locus_tag="IV142_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8483773.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(95587..96264)
                     /locus_tag="IV142_000083"
     CDS             complement(95587..96264)
                     /locus_tag="IV142_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP8483774.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(96294..97385)
                     /locus_tag="IV142_000084"
     CDS             complement(96294..97385)
                     /locus_tag="IV142_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP8483775.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(97486..97920)
                     /locus_tag="IV142_000085"
     CDS             complement(97486..97920)
                     /locus_tag="IV142_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8483776.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(98504..99961)
                     /gene="ascB"
                     /locus_tag="IV142_000086"
     CDS             complement(98504..99961)
                     /gene="ascB"
                     /locus_tag="IV142_000086"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8483777.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(99958..101853)
                     /locus_tag="IV142_000087"
     CDS             complement(99958..101853)
                     /locus_tag="IV142_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8483778.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(101911..103686)
                     /locus_tag="IV142_000088"
     CDS             complement(101911..103686)
                     /locus_tag="IV142_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8483779.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(103736..105232)
                     /locus_tag="IV142_000089"
     CDS             complement(103736..105232)
                     /locus_tag="IV142_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8483780.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(105426..107048)
                     /locus_tag="IV142_000090"
     CDS             complement(105426..107048)
                     /locus_tag="IV142_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP8483781.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(107497..108810)
                     /locus_tag="IV142_000091"
     CDS             complement(107497..108810)
                     /locus_tag="IV142_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP8483782.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(109055..109279)
                     /locus_tag="IV142_000092"
     CDS             complement(109055..109279)
                     /locus_tag="IV142_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8483783.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(109299..109667)
                     /locus_tag="IV142_000093"
     CDS             complement(109299..109667)
                     /locus_tag="IV142_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8483784.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(109864..110574)
                     /locus_tag="IV142_000094"
     CDS             complement(109864..110574)
                     /locus_tag="IV142_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8483785.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(110592..111089)
                     /locus_tag="IV142_000095"
     CDS             complement(110592..111089)
                     /locus_tag="IV142_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8483786.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(111126..111578)
                     /locus_tag="IV142_000096"
     CDS             complement(111126..111578)
                     /locus_tag="IV142_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8483787.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(111713..112318)
                     /locus_tag="IV142_000097"
     CDS             complement(111713..112318)
                     /locus_tag="IV142_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP8483788.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(112768..113388)
                     /locus_tag="IV142_000098"
     CDS             complement(112768..113388)
                     /locus_tag="IV142_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8483789.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(113390..114259)
                     /locus_tag="IV142_000099"
     CDS             complement(113390..114259)
                     /locus_tag="IV142_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP8483790.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(114282..114689)
                     /locus_tag="IV142_000100"
     CDS             complement(114282..114689)
                     /locus_tag="IV142_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP8483791.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(114708..115997)
                     /locus_tag="IV142_000101"
     CDS             complement(114708..115997)
                     /locus_tag="IV142_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8483792.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(116024..116326)
                     /locus_tag="IV142_000102"
     CDS             complement(116024..116326)
                     /locus_tag="IV142_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8483793.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(116351..118444)
                     /locus_tag="IV142_000103"
     CDS             complement(116351..118444)
                     /locus_tag="IV142_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8483794.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(118933..119697)
                     /locus_tag="IV142_000104"
     CDS             complement(118933..119697)
                     /locus_tag="IV142_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP8483795.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(119808..120677)
                     /gene="mdcB"
                     /locus_tag="IV142_000105"
     CDS             complement(119808..120677)
                     /gene="mdcB"
                     /locus_tag="IV142_000105"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP8483796.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(120702..121778)
                     /locus_tag="IV142_000106"
     CDS             complement(120702..121778)
                     /locus_tag="IV142_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP8483797.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(121802..122395)
                     /locus_tag="IV142_000107"
     CDS             complement(121802..122395)
                     /locus_tag="IV142_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP8483798.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(122399..124075)
                     /gene="mdcD"
                     /locus_tag="IV142_000108"
     CDS             complement(122399..124075)
                     /gene="mdcD"
                     /locus_tag="IV142_000108"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP8483799.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(124065..124364)
                     /locus_tag="IV142_000109"
     CDS             complement(124065..124364)
                     /locus_tag="IV142_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP8483800.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(124466..126112)
                     /gene="mdcA"
                     /locus_tag="IV142_000110"
     CDS             complement(124466..126112)
                     /gene="mdcA"
                     /locus_tag="IV142_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAP8483801.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(126119..127030)
                     /locus_tag="IV142_000111"
     CDS             complement(126119..127030)
                     /locus_tag="IV142_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP8483802.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            127150..128007
                     /locus_tag="IV142_000112"
     CDS             127150..128007
                     /locus_tag="IV142_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8483803.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(128194..128556)
                     /locus_tag="IV142_000113"
     CDS             complement(128194..128556)
                     /locus_tag="IV142_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8483804.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            128902..130341
                     /locus_tag="IV142_000114"
     CDS             128902..130341
                     /locus_tag="IV142_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8483805.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(130348..132900)
                     /locus_tag="IV142_000115"
     CDS             complement(130348..132900)
                     /locus_tag="IV142_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP8483806.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPKGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYELGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(133014..134345)
                     /locus_tag="IV142_000116"
     CDS             complement(133014..134345)
                     /locus_tag="IV142_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8483807.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(134311..135789)
                     /locus_tag="IV142_000117"
     CDS             complement(134311..135789)
                     /locus_tag="IV142_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8483808.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(136094..137929)
                     /gene="tdc"
                     /locus_tag="IV142_000118"
     CDS             complement(136094..137929)
                     /gene="tdc"
                     /locus_tag="IV142_000118"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP8483809.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        40421 a        28686 c        24058 g        45047 t
ORIGIN      
        1 cgattgttaa gctcaatttg tttgctagca tattgcttgc ttgttaaaaa atgtttgttg
       61 tcttgaattg aatcaagacg ccctacacat ttggtttgtc ttactttgtt cagttttcaa
      121 aggtctactt catcaggagt gttccccgat tcgtttttgg ctgccgtagc aacttttata
      181 tcatatcaga gagacaagta attgtcaact cttttttaaa agtttttttg atttcgtttt
      241 tcgaaatctt acagcaactc atttatcata acttatcatt tgttatttgt caagaacttt
      301 ttttgaagtt tttttcgata gaatcaagct tcacaagtgg ttctttttta acaactcagt
      361 tatcttatca tgtcatttct aacatgtcaa caactatttt atatgttttt taaaatacta
      421 ttttattgct ttgctactta aaacaacaga aatgaatata tcatgtaaat ttacacatgt
      481 caatatattt ttaactctat atccccacct agatttgaat tttgaccaca taaattgagc
      541 atgtctctat agtagaaaaa taaagtcaaa aaaagaacac ataacacgct tgatgctaag
      601 tgttcgactg ttacaactgt atgaaatgct cagtgacttg attaaaacag ttaaagtcta
      661 tttatttaag ataagctgtt ccatcaactg ctcattgtat ccaataaata gaacttcatc
      721 gataataatc aaaggacgtc tgaggagatt ggtatgttgt gtgcataaaa aaatcagttg
      781 tttttttcct acacaggaaa ggtcaatcgc aatgttgtga aaagcctcag accaagtcgc
      841 aaatagctgt tctgcttgca cttcttcaaa actgcataat tcttctgctt cttctgtact
      901 tagatcgcgt tcttctaagt caacaaattg ataaggcaat tgatggtgtt ctaaccattg
      961 tttcgccttc agacttgtta cacagtgcgc tgatccatag actttcactt ccacgaaggc
     1021 tccctcccta tgaatttaag atgttattgt tttgtaaata gatcagattt ctggcaacac
     1081 atcgaatttc acgagggaca aatgaccgga tctcatcttc attaaatccg attagtattc
     1141 ttttttcatc aatcaaaata ggtcgtctca ataatgaagg atattctttg aataagacga
     1201 tcatttgctt taaagttaat tcgttaaaat ctatgcccag cttttcgtaa attttcgacc
     1261 gagtggaaat aatatcttga actcctcctt cagtcgctgc caagatcttt ttgatctcac
     1321 tttctgttaa cggttcggca aaaatattct tctcttcaaa ttcaagttga ttcgcttgca
     1381 accatgctct tgcttttcta caggaagtac agctaggcga agtatatact tttatcattt
     1441 tctcactcct ttttaattat aatccaattc tacagtaatt tcttttaaaa taccaattat
     1501 tagctaataa aatataaaaa aaaatcaaaa taactgatta tttacgtttg aataatgaga
     1561 atttttctat atattctcta ttttgataat agtattctca ttttcaaaat aaataaggga
     1621 ggtgtgacaa aattcttgtc acacctccct tttccgaata aactgtgttc cagaaatagc
     1681 ttcttcgaaa ataagctgaa atttcacaaa aatttgagaa gcaattttcg tacattcctt
     1741 cttctttctt gaaactgacc acttctgtca caccctctct tctatcactt tccttttttc
     1801 acacgactcc cggtttcttt gataaatctc gatactttca attttcttga atttttcatt
     1861 tgaggtacac atagatacag acgatttctt gctctagtca tcgcaacata aaatagtctt
     1921 cgttcttctt ctatcgtcgt cactggttct gcttttattt cattaaagtc gattactttc
     1981 ttttctgtcg ctccgttata aggcaaactt ccttcgttac aaccaatcaa aaagacattt
     2041 cggtaactaa gacctttact tttgtgaatg ctctggatac atagtgcatc aagttttcct
     2101 tgtctgtaag caatcaagcg ggtttttaat tctttagctg tcgcttgggt ttcttgcgat
     2161 aaggcttgga gtcttctgaa agtagcatgc tgctttactt cttctcgtat ccattcttga
     2221 tcactctggt cgatttcctt agctatacgt ggcagatgaa gatagctttg aacaagcgaa
     2281 tagatttctt gatgctggtc tgttttacgc aacgcttcct caaactctcg gatttctgca
     2341 gatttcgatt ttaaattggg gcttatcgac aaaatataat ctgcaacggc tatattttga
     2401 tatttaggag aaaagtattg atcgcattca ctttttttca ggtagcagtc tgccatcagc
     2461 tgccggagca attgctggta ggcctgattt ttcttttgca gattcagtgc tgtttgccaa
     2521 tgaacaattt tttgatagaa atgttggaaa gacttgatac caaagatacc ttcatatgga
     2581 ctatctgctt ccagtacaaa aggaatattc gttttcagca gagattggac aagctgctgc
     2641 gtttgtgaag ctgatcggta taatatgatt gattccttat atttcccatt tttttgaata
     2701 aatgtctgaa tttgtttgat tagccactga ctttctgctt tagtatcaga aaacaaatag
     2761 gcttccataa cacctgcttc tttttgtgct ggaatcatcg cctttggtat ccgatcattt
     2821 ttattatagc tgatcaatct atttgctcct tggacaatag ctgcccgaga acgataatta
     2881 gttaataaga aaagagtttt ggtaggatat tctttttcaa agttcttgat gtatgctggc
     2941 tctgatcctc tgaatccata gatactctga tcatcatctc caacaacaat caacttgatc
     3001 ctgttttcct ttgtcatccg tttcacgatt tcccattgaa gtggattagt atcttgaaat
     3061 tcatcaatga atagataatc atatttttgg ccgatataat gctggatctc ctcattctcc
     3121 aaagcgcttt tcagattttg gagtacatca ttgaactcta gcaattgctg gcttcttttt
     3181 tgttcttgga gttccgtgag caatccatac agtcgttctg atatcggctg tcgtgcattc
     3241 ttatcaatgg aatcatattt ttctttaact aactgaacca tttcttctac tgaaaggttc
     3301 atattctgcc aatagtctaa ccgagaagca atcgtttttt ctccatattg atcaaatata
     3361 taaagattat atttacgtat cagctgttgc aaaacagcat tcactttttg tgcagtatcc
     3421 tttgaaaagt ctccttgtat tccttgcatt gctacttcag gaaattttcg acaaatttca
     3481 ttgtatatcc taaaaaagat actatggaat gttccgatat ggacagcggt ttttctctgg
     3541 ctgaaattat ctactcgttt ttttagttca tttgcagcgt tccttgtaaa agtgatcatt
     3601 agtatgcgtt cttcgtctac ttgtttttgc gtcatcagat aaacagcttt tgcacagatt
     3661 gtttgcgttt tgccgctccc agctcctgct atgattcgaa gatgtttctt catatcatat
     3721 gtgatagctg ctttttgttc ttgattgaag cgattatagg aacgtttctc catcatttta
     3781 aagaaagagc tgtttccttc actttctttt acttgtactt cttcctcaat aagtccgggt
     3841 gcttcgccat aagccgcttc ataggtagca atgatttctt cttgtagccg aaagacttgt
     3901 tgaagctttt tatacttgtt ctcccagcgt ttcaattctt tttgtaatcg gagaatttct
     3961 ttttcattca actgattgat ttccttccca tctttcttct ttgaatttgt ttatttcaag
     4021 ttggttattt aaagtttcgt tgtctatttt ttctttatac taacagacct ttctaaaaat
     4081 gaaaatggga agcgatatga ttttagtttt ctgaaaattt ttcctttttt tcaatcgttt
     4141 atgctaagat aaggaagaca aaaatattca taaaaggtga tgaaaaaaat gcagacttcc
     4201 tttcacgagg ggcgtacaat aggacttttg ctgacattta tcggaggagc catggacagt
     4261 tatacttata tccaatatgg tgcttttgcc tctgctcaaa ctggcaatat cattctatcg
     4321 atcatccaag catttgacgg agaatggcta agtgttggta aaaaagtatt gtctaccctt
     4381 ttctttttct tagggattct tctaacaaag tatctaattg attattttag aaaaaaagaa
     4441 aaacatttct ggcgcttgtt tgttctatat tatgaagcag tgattttttt ctttgttagt
     4501 ttgacacccg tccatgagca tcccgcatta gtaacgattt tgattgcttt tactgcatcg
     4561 atccagtggg tagcttttga taaaatcaac gggcgtgcct acactaattt atttacaact
     4621 ggaaatttaa aaggggtagc aacgaactta tatgattatt gtatttctaa agaaaaagca
     4681 gattttgatc gatttttcca ctatctaagt gtcgttcttg cctttatcgc aggagctatt
     4741 gtccttgttt tctgtcatca tctgtttggc gcccattcga ttttgatcgt ttccatttta
     4801 tttttgatca tggccctcac tcagacattt catttatggc aattttatca tacgaatcgg
     4861 ttcactattt gatctcaatg aatcaaaaaa actaagcaag atgctgctca cagagaaata
     4921 tctgcgagtg agcaatttgc ttagttttta tattatttag ttgtaaagat tccctttgct
     4981 ttcgggttat ttgtagtgta gcggatcgac tgggcatctg cctcttctgt cattttcttt
     5041 gcccgcatct tgatttcttg tacgatttgt tctccacatg ccggatcggc tacgaaatca
     5101 tatttgtccc catcgatcac tattttggga catatatcaa actgttcata ccactcttcg
     5161 tatcgtcgat tgagttcttt atagtaggaa tagagttcag gatcttgttc tagctgttca
     5221 tattcccggc ctcttttttt gattctcgct aacatagttt caaaggatac acgaatataa
     5281 accagtagat ctggtttctt ttgtggcgaa atttcttcta actccttcag catcgtttct
     5341 aaaagacctt catactgctt tacttctact tcagaaacac gccctaaatc tgcattcaaa
     5401 tgaaacagca tactatcttc gtagatggag cggtctagta cgttgtcatc atggctgaaa
     5461 gcatctttga ttgccaaaaa gcgtttgttc aaaaagaaaa tctgtaataa aaaagtatac
     5521 ttttctggat tagaataaaa tagcggcaat acttcattat cttccacgtt ctcataaaaa
     5581 ggttttgttt ctaaatcttg ggcaagcatt tcagttagtg tgcttttccc tgcaccgatc
     5641 gttcctgcaa caatgatcac aattatcggc atccttcctg taataaaatc tgttcttcat
     5701 tttactactt catttataga aataaaagcc ttgactttat ctgctcctta aaagctactt
     5761 ctctaaaagt agccaaaagt aactcctatt catgaaaata aaggctgtga caaaagtctt
     5821 gtcgcagccc ttttttcaaa taaacagtat tctcctgcca acacctcggt cacaaggcaa
     5881 tattcataaa acatgaaaag ctgtttttta acattattcc ttgtaactca gagctaaacg
     5941 ggagactcac aacctcttta tgctctcaac attttatctg tttttattct tctattgtac
     6001 ttgatttgcg tacaactaac tgccagtgca gattatgagc ggtatcattg tcttctactg
     6061 cgcatttcat gacacgtatc atcacactat tttcatagat tttttctatt tttcctttta
     6121 ttggtgattc aaataaatca gatttaaatg catatgtttc gccgacttca aacaattgtt
     6181 cattttccat aagaatccct ccatcacctt ttataaggtc agtaattttt taacataaat
     6241 cactcgaaaa agcacaaact tgtcataaaa agtacttttc gtatcaaaac tgtcactttt
     6301 tttggtaagg ttcttggaaa acgaagattt ttttaggaga tcatgacggt tttgatgtca
     6361 aaaccacttt ttatgaaaca aaagacgttt tttatttttt ttagcataaa atttgtggtg
     6421 gaggtacgta tgagatgaaa gataaagttg tgaacaaatt attagaagcc ttattcagcg
     6481 gagacaaatc ggaagaagat atggtgtatg tccaagtaaa atttgcctta gaagttttat
     6541 tgaataatct tggaaaacta gcggtcgttc tagtcttttc tctggtgaca ggctcatggg
     6601 cggaaacagg aatcaccttt ctttcttata tctgcatccg cagatatgct tatggattgc
     6661 attccgactc tgaattcgtt tgtctgctgt ggacactact ttatttatgg ggcgttccct
     6721 tggtgatgaa acatctgcaa ttgactatct ccttcccaat gatggtcttt ttgttaatca
     6781 gctgcttttt gttactgctt cgctatggat caagaggtac agcaatcaat cccattgaac
     6841 ccgagaaacg tcccccatta ttaaagaagg cgatcagcat gtttctaatt ttttctctca
     6901 ttactttatt tttctcagct tcttactttt ctacctattt gctacttggg attgttttag
     6961 aaatagctac gttgcttcct attacaaact acttgatgaa ttttggagga atctttcatg
     7021 aaaaaaacaa tcattaaaca attaactaac ttatctgact tttttgtatc tggctgctgc
     7081 tttggtatga gttctgaatt caaaatccct gaaaaattaa aaaaataata cgctaattgt
     7141 gctataattt ggtaaaaatc aatgggagct gtactttatg tttttatggt ccatagcgtt
     7201 gcaatatttg atttacacaa tttgtatctt attgattgac catcacatcc gatacaaata
     7261 tctgtttgcg ggagtcattc tgatttcttg tattttgatt ggatgggtct attcgatttt
     7321 agggattttt actgctctaa ttgctatagg aatgctgatg cttacttttt tcttcttttc
     7381 gacttcgcgt caacaattgg tttacgcgct tccttttggg ttattgacgt cgcttttagg
     7441 agatcactta acaagcatcg acgatttttt tgttttgaaa tcttcggtta ttgaaccagg
     7501 agattcattg cagtattttc acattgtctt atccgctttt ttatctgctt tattagcttt
     7561 ttgtttaaaa aaatcgttag agacttggtt tcacactgtg gaccgccgta tgatcggtac
     7621 gatcgggaca ttgatattat ttacatacta tatcattatt ttttataccc gtttttctgg
     7681 tgaaacacct gatgtcttag ctttgaatac gagttttttc attctatatc tgtgtgttgg
     7741 cctgatcatc ttggtttatt attggaaaat ctctaataag aaattagctg atcagttatt
     7801 agagcaacaa gtcaggatgc aacaggatta tatcaaagat ctagaaaaaa actatcaaga
     7861 cttgagagaa ttcaaacatg attaccaaaa tctgcttttt tctcttcaca gctatatttc
     7921 agaaggcgac ttagaaggat tacggtctta ttatgatcaa acgatattac ctacacgcca
     7981 gatgatgaac ctttttccgg ccaatctaag tatgttggat aatatgaaag ttcctgagat
     8041 ccgtagttta ctgtcattaa aactcatgat ggcacaggaa aaagggttga tcgtccagct
     8101 tcattttcct gagccaatcg aactggatac gaaacatacg gtaaaccttg tacgtatgct
     8161 tggtattatc ttggacaatg cgattgaagg agctagtgct gcaaagaaga aaaaaatcga
     8221 attgaccata ctcaagaaga atcattccgt gatgattcgt gtgatcaata caacaacaaa
     8281 tacattaccg ttaaaccagt tgaaacaaaa agggttctca acaaaaacaa atccaaaaaa
     8341 cgaaggcctt ggcttgttta tcttggatga attgacaaag accaatcctt atcttttcct
     8401 tgaaacatca attgaaaacc agcgtttttc tcaaacgatt tatatacaaa taaaataaag
     8461 gagtaatgat atgattccca tttatatttg tgaagacgat ccaaaacaat tagaagtttt
     8521 atcaacggtg atcaaaaata gaatcatgat tgaaaactta gacagttatg tccatatttc
     8581 tacctctgat ccggaagaac ttctcaatgc agccaagatc agaacttctt cttttggtat
     8641 ttacttttta gatatcgaac tagaagacag tgaaatggat ggtgtagcga tcggtaaatt
     8701 gatccgtgaa ttagatcctt taggaaaaat aatctatgtg acttcccata cggaaatgtc
     8761 gatgaaaatc ttaaaaagca acatcgaacc gactgattat atcgtcaaag aagatcttta
     8821 tgatttaaaa gaaaatgtcg agcatatcct gagcaaaatt ttcgaagatt ttcaaatgtc
     8881 ccaaatccaa aaagatatct tcaaaataga gtttaatgat gagatcaaat ttttaccgat
     8941 caaagacatc caatattttt ctacttcgct aggagcgcca cataaactgg aagtccattt
     9001 gactcatgcc caattacaat tctatgagaa gatcaaagaa atcgagaaaa tgcatcggaa
     9061 ttttatccgt tgtcataaat cctatgttat caatacacaa aatgtgcggt cgattaataa
     9121 aaaaaccaga gaagtcacgt tggcaaacgg cgagaccatc ccagcctcta tccgtggatt
     9181 aaagaaattg atttcataaa aagaaccgaa tagtttggac cgactaaatg accagccgat
     9241 ccaaaccctt cggttctttt ttgcctatca gtgcaaaagc atttcctgtg caatttcacg
     9301 tccatccatt gtatctggat aaaatgttgg ccaattctcc atttctttca gcaatacttc
     9361 ttgactttca tttcctgcat ataaatgaaa atgttcggag tcagccggtg caatcgtgtg
     9421 atcactgaat tgaatatatc taaaagcgcc actttcctta tcttctgctt cgaatagata
     9481 ccttacgcca cggtttcctg cctcgtaggt caagatttct ttccctacat aacgataatt
     9541 tgaacgtttc gttgttccat ccttaaacgt aaatgccatc tctttctcac tgatcttgat
     9601 tgaagcaata tctgtttgat agcccttcgt ataatattct ttatattctt gtgctgtctt
     9661 atcctgattc aatttttctt tatattcaaa aacaggatca aaagtcccat cttctacata
     9721 cgtatctact gtttgccaag ttcctgccca atctgaaaga gagcgatctt gtacatcctc
     9781 gtcttcaaaa taaccgttgt agacgctctt ttcttttttg ggggtcaaag tatcttctag
     9841 cgggttttct gtctctgtcg tttttttcaa cgcttcgagg tttgcttcca tgatagaaag
     9901 atagttttct ccattttcta tttgttcatt cgtcaatcct tccaaaggat tcaatacttc
     9961 taaagataca cctgcttcgt ttgctaacgt tctggcaata ctatctttgg cgttttcttc
    10021 aaaataaata tagttgattc catgttcttt aacaaagtgt ttcagttcag caattctcgc
    10081 cgcagacggt tcttcactag aagaaagacc agcaatcgat acttgattca atccatattc
    10141 taacgctaga tagttgaatg cagcatgttg cgtgacaaaa gatctttgtt ttgcttgctt
    10201 aaaggtttct tgataaagct gatggagtgc ttgaagtttc tcggtatagg ctgctgcatt
    10261 ttttgtccat acttcctgtt tttctgggta agcttcaatc agctgatcac gtatgtttgc
    10321 tacctgcttg attgctaaac ttggagctag ccatacatgt gggtctagct catgtgtatg
    10381 gccttcttcc ccgtgttcat ggtcatgatc atgtccttct tcacttcccg gaagtaaaag
    10441 cataccttct gtcgctttta tcgtatggac attgccttct tgtaatgttt tttggattgc
    10501 ggggacccat gtttccatgt tttcatcatt ataaacaaag atatctgtat cttggatctt
    10561 tgccatgtct ttagcagaag gttcataatc gtgggaatca gtccctgctg gaatcaataa
    10621 ctctacttct ccttcatccc cgacgattgc ttttgtaaaa tcatacatgg gataaaaggt
    10681 cgtcatcacg ctcaattttt ctttactgtc ctcggctttc ccacttgccc cgcaagcagt
    10741 taacatacct gctaaaacca gcatggccaa tatacctatc aatcgtttca tttatgacac
    10801 tcctcttctc atcttctttc atttgaatgt tccgattcgc ttttcgcaaa ccgtaatagt
    10861 tacgttttag caacaggatt taatttatca tatggttaaa agaactgtca agcgttttct
    10921 tattttaatt gatgagaaag tttatatgaa tgattgacga tatttttgca atcaacctct
    10981 atcattttcc ttttataccc gcttttgtct ataatgaagt tatagataaa ggaggaaaaa
    11041 gagtgaaaaa agaaacagat acaacgaatc gtttgatccg ctttttagga ggaaaaacct
    11101 cttactatgt tttaggaata gttatcttac ttgcgctgat catttatttt ttccatcaga
    11161 tctcgtttat attcaagcct gtcggaatca ttatcgctac tatcttgccc ccattattgt
    11221 ttgcactgat cttgtattat cttttggaac cagtcatcaa atgtttgagc aagcaccttt
    11281 cgaggaactt gtcagtcatt atcgtttatg tgattatttt atttttattg gggcttggaa
    11341 gcatatggct tattcctttt cttgaagaac aggcaaagac attgatcagt agtttgccag
    11401 atttgtttca agattttcaa gagagtctcc gacaattttt ggagaaaaca ccttttgccg
    11461 attcatttaa tcaatttttt gcttctattg acgacgtgac gtctgatata gctggattta
    11521 ttggaaatta ttgggaaagt ggcgcacaac ggatcagcaa cattttttca actgttacag
    11581 ctatttttat tactttattt actggtccaa ttgtcgcttt tttccttttg agagatcctc
    11641 aaaaatttta tcagtcagtt ttagcgattg tcccaccagc atttcgtaca gattttaaaa
    11701 atttgacaaa gattgctaat caacaattgg gttcatttct aaaggggcaa atcattgctt
    11761 catttatcct tggagcagtc tactgggttt gtttcttact gatcggatta gaatttgcta
    11821 gtgttttagc tatttctgct gggttgttat gtatcattcc atatattggg ccgtttatcg
    11881 ccttctttcc aggtttgatc attgcattcc aagactctac ttttatggct gtcaaatttg
    11941 tcattgtctg gtttgccgta caacttctcc acggtgactt ggtgattcct cgagtaatgg
    12001 gtgatcgttt acagatccat ccgattacga tcttaatcgt tttacttgtc atgggtgatc
    12061 taatgggaat tgtgggcgtt atttttggta ttcctatcta tacgttagtc aaattactcg
    12121 ttacttttat tttccgtaaa ttcaaacaac gctacaataa attttatggc gataaaggag
    12181 agtatgaaaa ctcttcattt tctgaagaag attattttaa ataaaacaca aaacgtcgat
    12241 cttacgagaa tagctcgtag gaacgacgtt ttgtgttttt gttttaattt ttcagcaaag
    12301 caattgcctg gtatggtttt agctccagac tttgatgaag aacagtatcg gaataattat
    12361 cgataagaat attggcttct gtgaatcctt ctgggatggc gatccttgta tctttatcat
    12421 agaaatttgt taatacaagc agctgctgtc cttcaaattg gcgaagaaag ccataaactt
    12481 ccggatgatt tgcttcataa gcatgataat ctccttgtgc aacgattgga tatgtttttc
    12541 tcagccgaat caatttcttg taataggaca aaatagaatc ttcagaagat agttcttttt
    12601 ctacattaat ctcttctagc ttgcctccaa cttttaacca aggtttccct gtagtaaatc
    12661 ctgcttgttc tgtgtctgac cattgcatgg gtgttcgaga gttatcgcga gattttgctt
    12721 tgatccgacg aaacgcttct ttttctgtga atccttgttc aaggagtaac ttataagcat
    12781 tcaaactttc tacgtcaata tagtcggaaa tcgatgaata gtctgggtcg atcatgccga
    12841 tttcttctcc catataaata tagggcgtcc cacggttcaa atggatcatg gttgctagca
    12901 tctctgctcc ttgttttcga tactgatcga tagctacaaa tcgattgagc gcacgtggct
    12961 gatcatgatt attaaaaaat agagcattcc agccgttacc ctcactcatc ttttctcccc
    13021 atgtatgaaa tagtcgcttt aattctgcaa aatcaaatgg aggagtagac catttattcc
    13081 cattttcata atcgactttc aaatgatgga aattaaagac cattgataat tcttctctac
    13141 taggttctgt atacaagata caattttcga tcgtcgtaga gctcatttca ccgactgtga
    13201 tgatagtgga atccgtccca aacgttgctt ggttcaattc ttttagatac gtatgggtga
    13261 ttggtttgtc cgtatattca ggtttgccat cattttcttt attatttttc aattgttcgt
    13321 ctttaccgat aacattgata acatcgaaac ggaatccttt tacccctttt tccatccaaa
    13381 aacgaacaac tttgaatagt tcctctctta ctgcttggtt ccgccagttc aagtcagctt
    13441 gtgtccgatc atataagtgc aaataatatt tccccgtatc tccaaatggc gaccaagcac
    13501 ttccaccaaa ttttgatacc caatccgttg gctcttctct taaaatgaag aagtcctgat
    13561 aatgctggtc ccctgctagt gcttttttga accattcatg ttctgtcgaa acgtgattca
    13621 gcaccatatc gagcatgatt tctatccggt atttctttgc ttcggctatt agctgttcga
    13681 attcctccat cgtaccaaag ataggatcga tggctgtgta atcagagata tcataaccgt
    13741 tgtctttttg agggcttgga taaatagggt ttagccaaat catgtcgatg cctagatcat
    13801 tcagatacgg gagtttttct ttgattcctt ttaaatctcc aattccatca ccattactgt
    13861 ctttgtaaga tttgggatag atttggtaaa tgactttgtc tttaaatgtc atggatattt
    13921 acctccattc aactgattta ttacctacca caacttatat atacaagtta tcatttatta
    13981 ttataactga accgaaagcg tttgtaaaat ttatctttat acattctaag aggtctgttg
    14041 cccaataaaa aacaagagat cagacatgta tttgtctatc ctcttgttta aaatagcaaa
    14101 atattcttgt ttcttctata aatggtgttc aaaagctttt atctacggca atcagtcttc
    14161 tcaaatgtaa aacatgagga gctaatttta tttgagtatt ctttttgctc gatgcaggac
    14221 ggctttttta caacctctga ttcatggtgt tcaattagca gctgaccctc gttattagct
    14281 caaaacgctg atttcacaac ctctgttttt attcattcga tttcaacgca tcaatcatat
    14341 cgatatgctt taatttgaaa tgggtaacaa tcatcacaat aaccgtaaat acgatagtta
    14401 atcctgctga taagacataa gcccaccatg tgatcactaa tggaaagatg acattctcca
    14461 tagacgcttg ttgcaagatg aagtcagtca atatatatcc tacaccaaat cctccgatga
    14521 ttcctaaaag agtaaagatg atattttctc gaacaatgta catcgtgact tctttatcaa
    14581 aaaatccaag gacttttatt gtagataatt cgcgtacacg ctcggaaata ttgatattag
    14641 ttagattata caagacgata aaagccagca gtcctgataa tacgacaaag attaagacaa
    14701 ccgagtctaa gttcgccatt gattcttctt gcgtttcgat ttgtttggac atgaaagacg
    14761 tatttgtcac agcgtctgtg gcaagcagtt tctcggaaag catttcttct tcttttgttg
    14821 tcatcttatc tgtacgcagc aaaaatgcat tatccatcat cgattgatca gtaactgaag
    14881 cataatattc aggggacata taaataaagc tacctagata gtttttcacg atttctttta
    14941 cttcgacttt ccattctttt tgatcttcat cataaagcga aatatgatct cctgtagata
    15001 agtcaaatag ttctgctact ttttgcgtaa tagcgattcc tgttttaggc aaggagcgtg
    15061 ggttctcttc aagatcagtt aaatggataa aacgatccaa atcagctgtt ttacttggta
    15121 cgatcactgt cagactttgc gaagcatgtc ctttttttct caattctacg gtttgagtat
    15181 gaatagctag gatctcattt actttttctt gcttctgcag catatccgct gctttttgac
    15241 gactatcttc cgtggatgaa tccaatgtaa cgattgcttg atagtcaatg atcggtccaa
    15301 attgtttggc tgaaacagaa cttagagaat ctttcaatcc cacgcctgca accatcagcc
    15361 cggcacatcc agcaattccg ataatcgcca taagcattct tgacttatat cggaagagat
    15421 ttcggtaact gattttttga ttaaagctaa ggcgtgacca cagcggagtg atgtattcaa
    15481 gaaaaatgcg tttccctggc ttcggtgctt tcggttgaag caatgcagca ggccgttctc
    15541 ttaaatcttt gaataaaacg atcattgcag cgccaaatgc ggcaatgaag aatgcggcag
    15601 ctgcttgtaa aatcggcggc cagtcataaa aaataagcgc actcccaata tcataccgct
    15661 cgttagacag ctcaaaaatg atccttggca aaagattcgt tcctaatatc gttcctaaaa
    15721 caatacctat ccctgaggct agacttgcat agattgcata tttttttgcg atttcaactt
    15781 tcgtataacc taacgccttt aatgtcccga tttccctgcg gttttcttct accatacgtg
    15841 tcattgtcgt aaaggtgatc aatgcggcaa taaagaaaaa gaaaacagga aaaacattgg
    15901 caatcgcagc gatacgatca gacagatcac caaattcttg aaaaccgaca ttttctgaac
    15961 gttcattgta taggtatgat ggtttagtta aattttcaat tttttctgca tttcgattca
    16021 attcttcctc tgcagaggcc agttcacgtt ctttctcaac taattgcctt tcttgttcag
    16081 caagttgagc ttcttgttga gataatgttt gaacagcgat tgttcgttgc gcttcaggaa
    16141 gctgctggat agttgctgac tgttgcgcta actgtttttt agcatcttct aaccgtgttc
    16201 ttgcttgcgt tgtttgtgct ttgccatccg ctattttttg ctgtgccggt tctaattctt
    16261 cgttggccga ttttttcagc tcttctaagc gttcttcggg tcgatcttgt aaatactttt
    16321 ctgccttttc ttgattttgc tccattttat ctttgtaagc tttgctgtac gtattcaaat
    16381 tctttacgtt tgtaaaatca agatataaga cagaataaat atctgtttta aaaacggatt
    16441 caggtaaata gacaaaataa tcgacgctac catttccgac gttcgcgtat cctctctcca
    16501 aattgttgat gaacaatgga gaacggacaa agcccgtgat ggtatactct ttttctttca
    16561 attcatctga atcaatctga taaatatcac cgattttata cccttcatct tccgcttttt
    16621 catccaatac aagctggttt ggtttttccg ggagttttcc atcagttaat tgtagcttgt
    16681 tctgcttgtt atttttgtta taagaaaata tttgaatcac ttcgtttttg ccagtattgg
    16741 aataatagat ctgttttcct gtttctactt ttgcatctgg gatcttttct gcttgagcaa
    16801 tatctttttg tgtcaagcca ccagtagaaa cgatttgcaa atcagaaagt cctgctgtac
    16861 caacatagtg atccatcgtt ttttgcatga caggtccagc agttttgaca cctacaaata
    16921 aaagtgttcc tagcatgaca ataaggatga ttgcaatgaa tcgtccttta gattgccgaa
    16981 tttcgcgaaa acctgcttta agatatgttc ttgttttcat aaaatccccc taccatacaa
    17041 tctcatcaat ggatgaagga tgatcattta attcaacaga ccttacttta gcatcattaa
    17101 tgtgaatgac tctatcagcg atgggcgcta aagcagaatt atgtgtaatg atcaaaacgg
    17161 tattgccgtg ttgtctgcta gcgttttgca ataatttcaa tacttgcttg cccgtttcaa
    17221 aatccagcgc gcctgtcggt tcatcacata aaagaagttt gggattttta gctagtgcac
    17281 gagcaattga aaccctctgc tgttcgcctc ctgataattg ggacggaaag ttattcaatc
    17341 gatgagctag gcccacttgc ctcaatacct ctacaggatc aagcgcatca ggagaaactt
    17401 ccgttgccaa ttccacattt tcttttgctg ttagattagg aacgagattg tagaattgga
    17461 aaacgaaccc tacatctgtt cgtcggtaag cagtaagctg tttatctgaa aattgcgcaa
    17521 tatctgtgtc atcaataatg atctgacctt catcaggtgt atccatccca cctaaaatat
    17581 taagaatcgt tgactttcct gctccacttg gaccaagtat aatcactaat tcgccttttt
    17641 caattgaaaa agaaatatcg tcatttgctg taatggtcgt ttctcccatt ttgtatcgtt
    17701 tgtattcatt ttttacttcg acatacttca ctctgattcc tccattttca cgatatttct
    17761 cgctatatct atgcatcgaa tgcgttttct cacacttatt tattttacca ctgaatttct
    17821 ctagactaaa gaaaggaatc ctttaattta gttgacaaat aaacgattta tgatttactt
    17881 acataaaaaa cattgttatt tgaaagaagg aacatcatga atgatttaaa ggaaaatatg
    17941 atttttgatt tatcaaaaac tgcttttgtc gcgatcgaca tgcaaaaaga gatcgtaaat
    18001 aacggtacac tctctccaca cactgcagca tctattctga caaacaatga tttgttagta
    18061 agaacattga aacgtaccgc tgccttgaaa gttttagtga acgtagacat ctccactttc
    18121 ccttatttga gtcaacaaac tgatatgggt ggaatgactg cttctgtacc tccagaattc
    18181 actgatttac ttttgaagga ttctttgaaa gatacagata atatgttgac tatcacaaaa
    18241 tacaatccaa gtgctttttt tggtacttct cttgatcttc aacttcgccg gcgtggaatc
    18301 gaaactatca ttttaagcgg tgtagcgaca acaaacggtg tatatgctac tgcattagat
    18361 gcctttcaac acgggtacca tatcgtgctt gctgaagatg cttgcagtga tcgtgacaaa
    18421 gaatcccatc aattgttcat aaaaaagata tttccgaaaa ctgcacgagt acgctcaacg
    18481 aaacaaataa tcgaagctat ccaacaaagc aaataagtac tggcacacgt gattttcatt
    18541 tacttctttt tgatttcatg ggaaaataac cacaggattt agataggagt atgaacatgg
    18601 tgaaaaaaaa gatcacaatc aaagaagtcg ctaaactttc cggcgtttca atcacaactg
    18661 tctccttgat attaaatgga aatacggaga aattcagtcc gaaaactgtt cagaaagttt
    18721 tagctgccaa agaagaatta ggctatcagc ctgactattt tgctagacaa atgattacca
    18781 aggaaacgaa aacaattggt gtactcgttc ctgatatcac gaatccattc ttcagtacac
    18841 tgatgcgagg aattgaggat atcttgtata agcagaattt cgtcacgata ttatgtaatg
    18901 ctgattctga tcatcaaaaa gaaatcgaat atctagccga gctcacacgt cgaggtgtgg
    18961 atggattcat tatcgcgact tctgctgtct ctacagatgc aatcaacgaa aatttgaaaa
    19021 aacaaggccg gcccttcatt gttttggatc agaaaaaatc tgaaggattt agtgatgccg
    19081 tacgaacaga cgattttcgc ggtggttacc tagcgggcat gcacttgctt agtctaggac
    19141 atcaaacgat cgcccttgtc tatcctgaaa atccaccgga aaatgtccac gctcggattg
    19201 aggggtttaa atctgctctt gatgtttacc aaatccctca cgaccaactg atcctcttac
    19261 ctacacagtt ctcaaaacaa ggtggctacc agatcacagc agaacttctt gattctgctg
    19321 caactggtgt gtttgcttta aatgatgaat tagcttttgg attatatcgc ggattagagg
    19381 aagctggaaa aagcatccct gaagactaca gcattattgg ctatgataat atagatatgt
    19441 gcgagtatat caagccgaaa ctgacaacga tcgcacaacc aatcttcgaa ttgggacaaa
    19501 catctgccaa gttattgtta gatcggatcc aattcccaga aaaagaatgg gaagaaaaaa
    19561 gattacctgt acgattagaa aaaagatttt caactgcacc cttgaaatag ctttcaaggt
    19621 gtgctatatt tttatcgaac tattaaaacg ttttactaaa acgttttaaa aaataatttt
    19681 cccatcaaaa taaatgagat agttggaggt ttgtaaaatg aacacggtta cagtaattgg
    19741 aagtattaat atggacacta cccttcgttt aaccaacatg cccaagccag gcgaaacgat
    19801 gcatgcgcat gagatttttc acgctggcgg aggtaaaggt gccaaccaag cagtggcagc
    19861 caaacgttca ggcgcacgga catcttttat tggaggcgtt ggggctgaca gcgaaggaca
    19921 acagttatta gatctactga caaaagaaaa catcgataca tctgggatag ctgaaatcca
    19981 aggcgccaca accggacaag caatgattat ggtagacgca gcaggagaaa atagtatctt
    20041 gatccatgca ggggcaaata atgctttcca tgaacaagaa gtcctaaaaa acaagcaact
    20101 gattacaaac agtgacttta ttattgctca gtttgaaagt agcttagatg cgacgatcct
    20161 agctttttct attgcaaaag acgcaggaaa aacaacgatc ctgaatcctg caccagctcg
    20221 tgaaacgatt cctactgaac tacttgaaaa gacagatatc attataccaa atgaaacaga
    20281 aacagaaatc attacaggaa tcagagtgac agatcataac agtttggtcg cagcagccga
    20341 aaaactccat gaattaggta ttggaacagt aatcattaca ctaggcagtg ccggcgcttt
    20401 ctatcataca gaaaaagaac acggtatcgt ccctgctttt aaagtggatg ccgttgacac
    20461 aacagccgca ggtgatacat ttattggcgc attaagcagc acacttcagc cagaccttag
    20521 taacttgaaa gaagcgatcc tttatggaaa tctagcctct tctgttgctg tgcagtctta
    20581 tggtgcgcaa ccttctatcc cttaccgcga agctttagaa caatcagcag tctaaaatta
    20641 acaatgagag gaagaaacaa acatgaaaaa aggaaaagta atcaattcag atatttcacg
    20701 agtaatcgca caaatggggc actttgataa attaagtatc ggggatgcag gcatgcctgt
    20761 cccctttggt actgaaaaaa tagatttggc tgtctcaaac ggtcttccaa gttttttatc
    20821 tgttttagaa aacgttttag aagaacttga ggtacaatcg attgaattgg cggaagaaat
    20881 aaagacaatg aacccggaaa tattggaaaa aatcaaatta ttgttaccag atacaccgat
    20941 tagctttatc ccacatgaag aaatgaaaaa agagttgaat aactgtcatg catttattcg
    21001 tacaggcgaa atgacacctt acgccaatat tatattagtg agtggagtgg tcttctaatg
    21061 gaaacgacaa tgaatcctga acagatggta ctgtccgagc agattcatat gctgattgaa
    21121 cagttgggag gaaaagacaa tattgattct gtccttccat gtgcgactcg gcttcgtatc
    21181 aaattgaaaa aacagccagt agccaatgca gaaaaattaa aacagcaaat cccttccatt
    21241 aagtatttca ttttactcga tagatatgca gaaataatca tggatcaggg attggctcct
    21301 gcttacaagg aattgacgcg tcaaatcgat cattcagctc cgaaagaaaa gctaaatccg
    21361 atcgaaaaac tatttggttt gatatcggaa atctttacgc cgatcattcc gctgtttgcc
    21421 ggttctggta tattaaaagg tctggttgtt ttagcgacta cgattggctg gcttagtgaa
    21481 acaagcggaa cttatcatat tttgtctgct gcgtcgaatg ccgtctttta ttttctgcca
    21541 atctttcttg ctttttctgc tgccaaaacg tttaaagtga atggatatat tgcggctgtc
    21601 attgctgcag cactgattga accgaatctg acctctttac tttcagacag tggcaaagca
    21661 gctactgatt ttctggggat tcctgtagta ctgatgcagt atacgtctac tgtcatgccg
    21721 gcgattttag gtattttctt ttattctttt gttgaaaaat tcctcaaaaa atatattcgc
    21781 gaaaatatgc aattagtatt tgtaccttta ctttcattag ttatcacggt tcctttgacg
    21841 ttaatggtat tcgggcctgc gggtgtttac cttggcgaag gtttagctgc cgcaattact
    21901 tggatgatgg atattaacgg accgatcagt ggcgcaatca tcgctggtgg ttggaatatt
    21961 ttggtaatat ttggtatcca atgggctgta aaccctgtca tgatcagtaa tatctctgct
    22021 tatgggttcg accgaatcgt tccgttgaca ggagcagcca attttggaat ggctggtgca
    22081 gctttaggtg tatttctgcg aagtaaacgt tcaaaaaccc gttcgatctc cggttctgct
    22141 tttgcttcga ttttacttgc gggagtgacc gaaccaactg tttacggaat tgcgatccca
    22201 ttgaaaaaac catttgttgc tgcttgcatt ggtgcagctg ctggtggagc tgtaatggga
    22261 tttgcccaag tgaaagcgat cgcttttgta tttggtagtc tgaccacatt gcctgcattc
    22321 atttccggta cttttttctg gtacttagca ggactggctg ttagtctagt cgtagcaatg
    22381 atcactactc tcgttagcgg gtttgatgaa gacctaatgt cttatgaata gttaatgaaa
    22441 aagactgaag tatgagatgt ttatcacata tttcagtctt tttcttttta ggatcattat
    22501 ttttataaat ttttgtgata ctttccaaac tttggtataa tttacatatg cataaccatt
    22561 tttatcccca cttgtttatc cccaaacaaa ggtggtctat gcaataaaat gaacgtcaaa
    22621 gaaaaacgaa tgaatgctcg ttgcttattt acaaattgtt ttatttagaa tacacagaca
    22681 gatttatgaa aagaaaaagc ttttcgacac gattatctgt tccaaaaaat gtctaatgaa
    22741 tgctgaataa gacagaaaat caatgaaagg aaaaggacta gtttattatg aaaaataaaa
    22801 aaacgatacg tttgctagct gctacttggc tgattgctcc gtctgttttg tcgaccaata
    22861 ccgtatttgc cgtagataat caaatagctg atcaaacagt cacaacagat acgactacat
    22921 ccagctcagc tactagtgaa acaacggatt cttttacaga agacccaact ggaacaacta
    22981 actcaacaac aggatcgacc gagccaactg aaattgatcc ggctgatcca tcagaaaatg
    23041 aaaagccagc cgagacatat tcccttacaa ttgctccttc tttgattggt aagatccaac
    23101 tgactgcttc gacagcggat tcccctgaac agcaaccttt ggatattacg gaaagtggaa
    23161 cggtagaagg tttattagcg gaaacgaagg taacttacac tattacgcca gctgaaggtg
    23221 aacaaattgc ttcagtcgct gttaatggtg ccaaagaagt aaattatgat gcaaagacct
    23281 ttacagtagg tacgaacaat ttatctatta ctgcagaatt taaagctgga ccggatactt
    23341 caacagagaa accaactgat cctagtgaag aagtccctag tacaccagat ccggaaacgc
    23401 caaacaaacc agatgtgggt aataacaaca ataataatgg ttcaaataat tcaaacaata
    23461 ataacaataa ttcgaacagc gggaattctt cgaatcaaac aaacccatct ggatcgaata
    23521 acaatggaaa tactcagaga gaaccaaacc atccaaacaa tggttcatcg aatggcggaa
    23581 cgaacaatgc ttcatctcgt ccaaatacag caaatcaaag cggtatagag aatccatctt
    23641 ctgcttcttc tgattttgtt gtaaaatcac caattgatgc tgtattgcct acaaatacaa
    23701 cgaatgtcca acaagcaatc gttcgagaag cattcaagca tcttggaaaa ccatatgtat
    23761 ggggagcaaa aggtccaaat acttttgact gttctggttt gacttattac gtatatatga
    23821 aagcaaccgg tcattatatc ggtggctgga caggcgaaca gcaatatgca ggtacgcaga
    23881 ttccagtaag tcaggcccaa cctggcgatc tcgtcttctg gggaccttct tccggtgtta
    23941 ctcaccatgt aggtatctat attgggaacg gacaatttat ccatgcgcct caaccaggag
    24001 ataaagttcg agtaacttct atttcagatt tcacgcctga ttttgctgtc cgagtaaact
    24061 tagcaggttt acctgctgct tcaggttcgc tgacaggcgg ctcatctatt ttagatggat
    24121 tagatggctc gttccatttc agtcaaaacc aaacaactga tcagttcttg aaaaagattg
    24181 cagatgatgc acaggaaatc gggcaaaaag aaggaatcta cgcatcagtc atgatggctc
    24241 aagctatcct tgaaagtgga tctggaaaca gcttattatc aagcgaacca aatcataacc
    24301 tttttggtat caaaggatca tataaaggaa gcagcgtgac cttcaataca cttgaacaag
    24361 atagttccgg acaaagctac caaatccgcg cacaattccg taaatatcct tcttacaagg
    24421 aaagtttgga agactatgcc gacttgatca aaaatggact gactggcaat cctgatttct
    24481 acaaaccaac ttggaagtcg gaaacaaaag actacaaaga agcaaccaaa tatttggaag
    24541 gacgttatgc tacagatcgc caatattcac aaaaattgaa cgcgatcatc gaagcgtacg
    24601 acctgacaaa atatgatgaa ccgaaaaaag aagcagcaac aactgaagaa gcaaacgctg
    24661 aaatcctcaa tatgtctaag cgattcgatg tgccaatcac aggattagaa ttttcaacga
    24721 taaaacgcgt caacaacgct tcttccatct tccgtgtcgc ttcaatctgg gatttgtgga
    24781 acaattttac ggcaagacag ataccagaaa caactactcg tacagttaaa gctgcaactg
    24841 ttcctgtact aaccttgtta agtttagaca aatcattctg gcttgatctg cctttaaaga
    24901 aatagaataa attaaaaagg ggatgtgaca ctgtcacact ccctttttaa tttattctat
    24961 ttctttatat ttattttgta ttccgttaca aactcgcgta cttggttagg ttccagagtg
    25021 acatctccaa aactttcaag tcgctccgct cctggcagtt cttgagtctc aaatgtgata
    25081 ccgccatgat ttactaattt attcccgcgc atttccggtc catcttctcc aaaatttgct
    25141 gtaaaaatca cgattcctga agcatctgtt tttacttgga tcgatacttt ttcatctggg
    25201 cttgtcaatt ccgcagcatt tagtaaacca gattgttttt tcaacaaaaa tggatgatcg
    25261 atgccgccca ctatcattga ttggcgaaaa tcactggcaa agacctcacc taatttttta
    25321 ggacgttgga agtcaaatgc tgtgccactt actgaaacta cttctcctgt aggcgttgta
    25381 tctttattga gcgtagcaaa taatcggcta ttcaaccaaa gtatatgttc gtcgattgac
    25441 tgtgctggat caccggttaa attgaaatag acatgatttg taggattgaa tactgttttg
    25501 cggtcactga tcccttggac cgtcactcgc catgtatcat cgttcgtcag cgtataccgt
    25561 acttctacat ctaaagttcc aggaaatccg ttttccatat ctgggctagt ctgatagaaa
    25621 atgaccgatg cttcgttttc accattgatg actgtataat gccactgctt ggcttcaaag
    25681 ctaggtgcac caccatgtaa acaatgacca gtgtccgggt ctgtttttac ttcgaacgtt
    25741 tcatcatcta atttgaatgt gccattcgta atccgtccag ccatacgtcc gattgaagca
    25801 ccgatataag ggtctttttc tagatattct tttgcttcat caaaacctaa gatcaactct
    25861 cgtttttcat tatcgattat tgtttgaaaa tgaacaaccc gtgccccaag atcactgaca
    25921 gacaaagatg tcccattttg attcgtgatc gtaatcaagt gatagttttc gccaaaattt
    25981 tgttcattga tttccatgtt ccttgcaccc tcttttcttt catttgcact gattccagta
    26041 tacatggaaa ccgttttctt aacagcttat tccgtgagta acgagaaaaa atttactata
    26101 ttctaataaa agaccgtgaa aaaaaggttg tgacaaaaat attgtcacaa cctctttttc
    26161 cgaacaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa acgaaaaata
    26221 tgaggagcta tttttgcttg gctgttctta ttacttgatg caggacagct ttttcacaat
    26281 ctctttttca atacgattcc agtatataac catgtcgtca gtacatagta gatgaaatac
    26341 aatacgaaga agatcgtaaa cggaacccaa atatctttgt atggattttg catgaataga
    26401 ccaaacattt gtaatccgaa taagacatgg actacaccta agattcctgg tgctaagaat
    26461 aacacgccta cttctctacg gatcgattgt ttaagcaacc cgcgtcgtgt tcctatcttt
    26521 tcaagcattt cataacgagc aatgtcactc ttgcttcctg acaagatctt gaacatcaaa
    26581 cagctagcaa gcatggtcaa gaaagctaaa cctaggaaga atcccataaa ttcaaaacca
    26641 gagaatagac cattatacac ttcatacatt tcatatttct ggttgctgta ctctactttt
    26701 tgcaaagatg gattgttcat tttattttga gcaacaagtg tttcgattgg tttcaggttt
    26761 gcttggaagt cttttacctg aactagctgt aaagtggatt gtggcaagtt cagttgatca
    26821 aattctgatt gactgactac cttgatttct ttttcttgct gttccggcaa ttcatagctt
    26881 cgaagttcct cttgtacagt cagttcattc atttttttag caggaacttt ttctttcgtg
    26941 atcttcggcg gtgttccatc ttcatgtttt atcatgatca atggttgagc cgcaaattca
    27001 cttgcattat agtaaatcgt tcccgcatct tctttttgag tataagaagc agaaagtgtt
    27061 ggtgaaagtt ctttgatttt ttgctgatct tctgcccgag gattattcat cactagatca
    27121 tatccagaat tgttttctgt caagtctgca atttcatttt taaaacctaa tcctaccgtc
    27181 aaagcgccca gtgccaaggc aaacagcata gcgaccattg agagcatttg tgtatattca
    27241 cgaatacgaa agtttaactg tgacaatgtg aaattgttca attttttcaa tgaaatctgt
    27301 tctgatttct ttagtaatga taaaaagaaa atgatcacag aatggaaaat gaaataagtt
    27361 cctaatacga tcgtaactaa ggctacccct ataccaaaga cttgataggt ccccacgtgt
    27421 ttcatcatat agtaaccgca gcctagaaaa gcaattccca gtactacttc aaggaaaaac
    27481 aagaatttat gctgcttgat tcttgttggt gtctgatccg cacgaatcaa tgctaaaatc
    27541 ggttttttga caatcgaaaa agcattgaca acagcagcta ataaaaacaa aacagcaaaa
    27601 aacaaaacag tgatcaacaa cccgtgaata ctaaaaggtg taaaatgcga aatattgaga
    27661 tctaactgat ccactaataa tcggtggacg atacttgtca atcctacacc gacagctgaa
    27721 cccactaaag tagctgcgat ccctactaag aatgtttcag aaaaaatcat ctgtgcgatt
    27781 tttctgcctt ttgctcccag catcataaac attgcatagt ctttttgccg catcgtcatc
    27841 aaaaaggaat ttgcatataa aatataaacg aatgtgatga tccctagtaa aacagaaccg
    27901 aatcggaaaa tgataactgt agccgcaacg atcgtattac ttttcaaaaa agcttcgttg
    27961 cttgccattg attcaaacat gtaaaaaata gcagatgcca taactaatcc tgagaataac
    28021 accaagtagt ctcttaaacg gctcttgatc ccagtaagtg ataatttcca aagcatagtc
    28081 aatctccccc tactgttcca agtttccaag aatagatagt atttctcgat agaaggaatc
    28141 acggctttga tctccacgtt tcacttcttg atggataccg ccatccttga tgaataaaat
    28201 acgttggcaa taacttgcag aaaacggatc atgcgtcaca agaagaatcg atacatgatc
    28261 ttttgtattc aattcgtcca tcgtatctaa aagatcccgg gcacttttgg aatccagcgc
    28321 acctgtaggc tcatccccta atagaatcgt tggttgtgtg atcaatgcac gtgcggcggc
    28381 gacccgttgt ttttgtccac cggaaatctc agaaggatag ctttccaaaa tatgagagat
    28441 ggataaacgt tctgcaattt tttggacacg ttgcttgatt tcttttggtc tgacgccttg
    28501 taaagaaaga ggaacagcga tattttcttg tgctgtcaag ctttctaata aattgaaatc
    28561 ttggaaaata aagccaatct cattcgcacg aaaatctgct agttgatttc cttttaatgt
    28621 ggtcacatct ttttgattga ttcggatatg cccatctgtt ggtttatcta atgtggataa
    28681 aatattcaaa agtgtcgact taccagaacc agaagcgccc atgatcccaa taaattcgcc
    28741 tttttctaca tcgaagctga tgccgcttaa tgcttgcgtc tttttctcat tgttttttcc
    28801 gtatactttt gttacattct gtacttcgac tattttgttc atttctttcc ctacccttta
    28861 attagttttt ccagtgctgt tttcggcacg tctttttttt cgacttcttc atacgaagtg
    28921 acacctttat ttttgttcca ggtgattttt aaatatgctc ctttacgaag cggtcgagtc
    28981 aattgtccct tgaagtccag ttctttttcc ttaccctctt tcgtatagcc aggcaggata
    29041 tatctatagt tgactgtttc ttgtccagag tctgattttg aaactgtttt tgtaccatct
    29101 gtcgtaattt gtacatagta ttcttctcca cccataacta cgtaatctgc gattttcacc
    29161 cctgcaagta ccacaacgat gaaaacaagt agaccgatca gaagtttttt cactttttcc
    29221 ccttctctct tactttatat actgattata gaaaaaagcg aacaattctg ccttcgaatc
    29281 aactatcatt cttctttcaa ttttgtcatt ttctttaaaa caactaaaaa aagagttcaa
    29341 gacaataatc cgtcctaaac tccttcttcc tatcttacaa aaagtatgtc acttacggtc
    29401 tttcatgtcg cctgtattga taacaggttg cgttcctttg tctgtaatga ttgagacaag
    29461 taaattattg atcaattgga ctttccgatc atcggtgaaa ttgatttctt gtccatcttc
    29521 gatttgctct aaggccattt gagtgattgt gacagctcct tcaacaatgg tttgtcgagc
    29581 agacaaaatg gcttttgctt gttgacgttg gagcatggca ctagcgattt ctgttgcata
    29641 ggctaaatgg ttcaagcgtg tttcgattac ttcgacacct gctactgcta aacgctcttg
    29701 cagctctttt tgcaactcgt cagacactgc cgtcgtgttt cctcgaagcg tcaaatcatt
    29761 ctcattgaat gtatcatacg ggtattggct agcgatatgc cgaatggctg tctcactttg
    29821 gatttctaca aaatcttgat aatacgctac atcgaataat gcctttgctg tatcaacgac
    29881 tttaaagact actactgctg agatttcgat ggggtttcca tccaaatcat tgacttttaa
    29941 aacagaacta ttgaaattcc gaactttcaa cgaaacagtc attttttgtg ccaaaggtat
    30001 cgtaaggaaa aagccatttt ctcgaatcgt acctaaatat tgtccaaaaa acaaaattac
    30061 ttttgcttga ttaggactga ctacagttgc cgaacttaac aacaataacg tgatcaacca
    30121 aagaaaaata cttaaaaaaa gtgcccaaac tttcgcttgc cacataccta aatagaagaa
    30181 aaacaatcca actaaagcta atacgaccaa tccgacaatc cctgcataac cattgatata
    30241 aaatgctctt ttttctctca tgctacttcc tgcgctcctt tatccttcaa taaatatcta
    30301 atgttacttt tttaattttt ttttcgaata cgcgccatta caccttctag ttctaactct
    30361 ttgtcttcag tgatgtacca accgcttgcc tgtgtccctc cggctgcctg cggtaaataa
    30421 tccaattttg ttcgaaccca gtgcttttct tctgtaaaat agtcttctac caagacctct
    30481 agatccatcc ctgctgaaag aggcaaatct tcccgaacag atttaaagat aaatcgttcc
    30541 ttcttcccat catacttcaa tattcctgta tcgataatcg gtaaagaata ataatccagc
    30601 tctttcttca ccgtcaataa atgtttgaaa acttcatggt acacttgctc taattggcga
    30661 ccttctgaat tgttcgggta attaaccatc actttttctt tgtctggatc atagaaggtt
    30721 tggcttagtt gagtgatata agtatgtaat ctttccattt ctgtttttaa cttttctctt
    30781 tgaccttctt ctaccttcat atactctttc accctgtttt ctttttagat attttctttc
    30841 ttattttact atagttatgt gcgttaagcg atgaaaaaca gcaaagattg gaaggatctt
    30901 tgctgttttt tcttaaaata tccagttgag tacaggatca atctgtttca ttgtcctctt
    30961 gatccgtcgt aatattgatc gatgtgtctt tagcagtgat ctttagcttt ttctgtcctt
    31021 gttctaacaa agtatttgtt attggctggt agtctttttc aatataagat tcaccagacg
    31081 tttggatagt atagccactg ttttccatct catgcaaaga taaattgcca ccgtttagga
    31141 tgacttttcc ttctttgctg atcaaactgt cttctagatg gaagttgctg ttttcacctg
    31201 tgacgatcaa gttatccaca ttggcctcat ctaatcgaag tgagcctaat tcatctttcg
    31261 cagttattgt tcctcctgta aattcattga acgataccca gccatcatgt gtcttagtat
    31321 tcactttttt gattgatact tcgttgatat caattgcact attattccct tcgattgtca
    31381 attcagataa ttcatgatct gcagggacaa caatagtgat ttgtgcactt gtgtcttcta
    31441 aactaatgat tgcacctgtt gattttttga ctgcattcac agtcaacacg ccatttttct
    31501 cctttatttc tggcttttct gtcttatcat aagtcgtttt tatctcaaaa tccaatccgc
    31561 gtatgaccga aactgtttga ttttctgcat ggatgacgat tttgtttgca tccttgattt
    31621 tttttgtctc agtaaccata tcgatcaacc gaggaccatt atcccaagtc agactctttt
    31681 gagcgcctaa tgtataagtt gcacctgcta ggaggccacc agtaagcatc agaccaatac
    31741 cggtaatcaa aaaatgtttc aatctcaatt tatgtgtcct ccttcaaacg cccgctgaat
    31801 gcagatctct ttttgtaata aaacgtcgtc ccatccattt aacaaagttc atcatgacat
    31861 aaaaaagccc ttttgttcct ttcagcaaca gtggcgaaat aaatactgcg cttcctgtgg
    31921 cgaacagacc gacaccgata aagaataatc cactcagaat cgattgtcca aacacactga
    31981 ttcctgagat gatcatgaat aggccaacac ctaagatact gatacataaa acatagatac
    32041 taaaaatgag cgctagtaaa gcaaccaata gtcccccaat aaccaatata aaccccagtg
    32101 ctagtggaat gaggacagga gaagcaaata aaccgataat gattaaccaa atcaggcgaa
    32161 tttgtctttt tggatttttc actaactcgt tttctgcttg ttcttcttct gaaaagtaac
    32221 tgatttttaa ttttaaggca aactgctttg gtgtaccata ttcatcaatg atctgatcta
    32281 atgtttttcc agaatctaaa aaatattcct cataatactg gatcaaatca aaacgatcgt
    32341 cttcaggaat ttcttgaaga tgatccacaa cttcattcaa atattcattc atttgtgctc
    32401 tcctccaata accggtctaa agaatctcga aaaacaatcc attcatcaat aatttcttct
    32461 aaatgacgac ttcccattgg tgtcaacgag taatatcgac ggtttctgcc ttcaaaaggt
    32521 tcatcatagg tctcaagata tccttctttt ttcaatcttc tcaaaaccgg atatagtgta
    32581 gattcactga cagagagact ttcttgtacg atttttgtta aggcataccc atacatatct
    32641 tccttgctta atgctgctag cacagtacca tcaagaagaa cagttggaat ctggatgtgc
    32701 attacgtcac cttctattct caagtgtaat attttatttc aatctatatt atacaacata
    32761 taatatagtt tcaaaatcag tttgtcaaat acaaaaaaac tgactaaaag atttgatcct
    32821 ttttagtcag caaaaaatgg cttctctttg atttttatta taacagtggc ataatccaat
    32881 ttgtccacat ccagctcagt ggcatgataa cgatcattgc aggtatcatg tcagctactg
    32941 ggaacatttt cacgttgatc attctaaaac cggtagcgac catcaagaat ccgccgcaag
    33001 ctttaaaatc taagatcatg tctggtgtag tcaatggata aataaatttt gccaataaga
    33061 acaaaatata aaaaataatg aattggggaa ttgctatcac agaaacgaca taccctaaat
    33121 tacacgcaaa aatagctgct gtgaaaaaat caaggacaga tttagaaatc aaaatcgtac
    33181 tgtctcctgt catcccagaa tctaaacttc catagatccc cgtaccactt gcacaaaata
    33241 gaacaatgac cgtcactaat gtgctgataa attcatcgtg ggtcatacca agcttttcac
    33301 tagggaagat tttagaaata ggtacttgca tcagcgcagc ccccttattt atcccattac
    33361 ctaaatgaac aatcaaacca atcgctgttc ctatcactaa agcaaaaata acagccggca
    33421 tatttttcat cggcgcaatg gaatagattc ccatccccat cgaacacaca ccaaaaacca
    33481 tcgtcagctg tgctttaaaa tgttcactta atttatttcc tactaatccg ccaattagtc
    33541 cgccaaacaa aacggaacct acattgatca aaactccagt tggcatcaaa aaactcctct
    33601 ctttttctac gaagcgaaaa aaatcagaaa aatttctgta attattgctt tcgctgcaag
    33661 aaagattgta acagacagag aagattacac caattcattt tttgtgttaa agtattcttt
    33721 attggaataa gtaggaggaa tatgatggat atccataaat tcgaagtttt tcttgattta
    33781 gccgaaacaa tgaatttcac caaaactgct gatcgacaat tcaccaccca aggaaatatt
    33841 tctaaacaga ttctttcgtt agaaacagaa ttagacgtca aattattcga acgcgcccat
    33901 agaaaaatcg aattgaccga agctggaagc ttattgctcc catatgtaaa aaatgtcgta
    33961 gaacaatacc atgctatgca agaagtgctt gaaacctata cgaaagacaa caatctttca
    34021 ttaaatatct taacgatacc gacaatgatc aattataaag gtttttccaa aatcaccgag
    34081 tttttgaaac gccatcctga atttacagtc cagttgaaag aagtggaaag tgtcgaatta
    34141 acttacaatg acgccaatga acatagtgac acgatccact ttgctagaag tttccaggcc
    34201 ccaccaagtt cgattgagtg gttgcctact gaaacagacg attttgtagc agttctacct
    34261 aaaaatcatc ctttagctag taaaaagaaa ttggatctaa gcgaactgaa aaaggagaac
    34321 tttttgctcc ttggccccac tacgaacttg tatcaacctg tactagatct gtgtcgcgaa
    34381 gctggatttg aaccaaaaat ctcttataaa ggagcaagaa tcgacttgat catcaatatg
    34441 attgcaaatg acatggggat tgctattgtt atggaaaaaa cagtcaagaa tctattaaaa
    34501 gaaaatactg tgattctccc tatttctcca acaacagaga gttatcttgc atttactcgt
    34561 aaagtcggtg agcattcttt ggcttcagat acattttggg agtatttaaa tcaagaagaa
    34621 aactaagccc tacattttct gtccatctat ccacccaaaa aaagagagcg acgcatgact
    34681 ttcgctctct ttcttgcatc atgtccttgt ttcaatgaca cctctaacat ttctcgtagt
    34741 tcaataagaa agcagcgatt tttatgccaa aagaaccgta agaacatttc cctaatcaca
    34801 aaaaaacgac taaaaacccc taaaaacctt caatagaatt tttaggggtt ttattctttt
    34861 tcctattgtt tacttaaccg tatcattgct gtcgttccta ctccttcgat actgctataa
    34921 tcaatacgtc catcatgcag ttctagaatt tttttaacca gtgaaagtcc tagcccattc
    34981 ccagaaattt gacgacttgt atctccttga taaaattttt caaacattcg atctctggtc
    35041 tcttcattca ttcctacacc tgaatcggtc acttccagta caatctctgt ggctgtctca
    35101 aatagtttga tatgaatctg tccattgatt tgattgtact ttatggcgtt atccatgata
    35161 ttcagccaca cttggtacaa aaactcttca ttgcctgtgt aatttactgc agcaagttcg
    35221 atatccaatt ctagctgttc tttctcccat ttaggctgaa aaaaaacgat tacttctctg
    35281 atttgttcat ctaatcgata ctctttcttt tccagttgga tacgctggtt ttccagtttg
    35341 ttcaatttta agatgttctc tgttaattgt gaaagctgcg tgatactttc gataatccga
    35401 tataaaaata tttggcgttc ttcgtcagag agatttgggg cttgtaacaa ttgaacatat
    35461 ccttggattg ttgctagtgg tgttttgaat tcatgggata cactggacac aaagtcattc
    35521 ctcagtgtct cgatactatt gagttcttgg accataacgt tgaaatcttt atacagttgc
    35581 tgtacttctg ctactttctg ctgttcatcc attcgaatcg aaaaatctcc cgttgctacc
    35641 agactcatat ttgtccctag cgcactgatt ggatgcagga tacgccgccc aacaaaaatc
    35701 gatattcctg tacccacgac catactgaac cccgcaaaga cgaataacgg gtataacgtt
    35761 ggttttccag ccgattcagc aggcgagata gaatgtcgag acaataagaa aataaaaccg
    35821 agaaaacaca gcagtgtcac taagatcgaa atgaagacaa tactagcaaa atagatccat
    35881 aattgtgaaa atagcttttt tttcatcttt aaataaccgc cttatagcct agtccccgta
    35941 ctgtgatgat ttgaaaatct tccgtctttt caaaacgggt ccttaaacgt ttgatatgta
    36001 catcgatcgt tcgttcttct gtattcgtat ctaatcccca aatatcatcc atcaactgtt
    36061 ggcgagtgaa gatcttgttg ggataagaca gtaatttata cagaagcaga aattcttttt
    36121 gaggcagaat caacgaatgc tgatcttgta acactgtata agcatctagc tctaattttg
    36181 tttgtccgat tatcaagcta ctgctgtaat tgatctttga acgtcgaagc aatgcttcca
    36241 cacgtaggat catctcattg acatcgatgg gttttgtcat ataatcatct gctcctaatt
    36301 tgaacccgcg tttcttatcc tcaaaatcgg cctttgccgt aatcattaga acaggtgtgt
    36361 caaatctggt gctacgtaat gagtctagca agtcatagcc atccattttc ggcatcatga
    36421 tatctgtaat gattaattct atacgtgtcg cttctagttt tttcagcgca tcctctccat
    36481 tcaatgcggt gatgactgaa aagcccgctc gttccaacac agaacggtaa agcaactgta
    36541 attgcggatc atcttctact actaaaatac gattcattga tctccctctg ttttttgaaa
    36601 aatttctaga ttttttcttg tagttcatct tcagttaaca tttatttcgt atactacgat
    36661 cattgttctg atatacttat tagtataccg aatatttcaa tacgggagaa gggtgattat
    36721 gcttcaatta aaagatataa aaaaatatta taaagtcggc gaaaccacca ccaaagcatt
    36781 ggatggtgtg tctgtcgctt tcagacaaaa agaattcgta gcgattttag gtcctagtgg
    36841 ttctggtaaa acgactatgc tgaatgtgat tggtggacta gataattatg attccggtga
    36901 tatggtaatc aacggaaagt ctactaaaga ctttaaggac agtgactggg atgcctaccg
    36961 aaacaactct atcggtttca tttttcaaag ttacaacttg attggtcatc taggcattat
    37021 tgaaaacgtt gaacttggga tgacattaag tggtgtttct aaagacgaaa aacgaaaacg
    37081 ggcagaagat gcattgcacc gagtcggctt gacaaatcat atgcataaaa agccaaatca
    37141 gttatctggt ggacaaatgc agcgagtggc catcgctcga gcactagcca atgacccaga
    37201 tatccttttg tgcgatgaac caaccggagc gttagataca gaaacaagta tccagatcat
    37261 ggaattgatc caagagttat cgaaagaaaa attagtgatc atggtcacac ataatcctga
    37321 actggcccat caatacgcag atcgaatcat tgaattttct gatggtaata tcttaacaga
    37381 ttcccatcct cacatcgaac gaccaaaaga tgatcaattt aacttacgca gaacaaagat
    37441 gagtttttgg acagcgttaa agctttcctt caacaatatc cgaacaaaaa aaggacgtac
    37501 tttcttgacc tcctttgctt ccagtattgg gatcatcgga atcgcgatcg ttctgtccct
    37561 ttcttctgga ttccaaaaac aaatcgataa cacgcaagct gaaacgatgg cgaaatttcc
    37621 gattactatt tctaaggtaa cgactaacca gactcgtgac gatgccggat tgggtgcaag
    37681 caaagcggat tatccagaca gcaaaacgat tactgcaaaa gtcagcgaag aagataaagc
    37741 acagcacacg aataagattg atcaaacata cgtcgattac gtaacagaca tcgatcctaa
    37801 cttaagtaat aatatcggat ttacccgtac tacaggaatc aatcttttac gtgatgtaaa
    37861 tggcaaagta cagcctgtca gcttctcgaa tcaaaatcct gatacggaat ctctttcttt
    37921 atctagtacg atgtcagcaa tgacaggtgt gggagtgtca agtttcccta cacagcttga
    37981 cactagcaaa gaaaactttt taaaagacaa ttattcttta cttgccggaa gttatccagc
    38041 ctcagcaaca gatgtcgtgc tgatcgtaga cggcaataat aatactaata tcaacgcatt
    38101 gaagaatctt ggatttgatg tgaaagaaga cgaaaaactt gattttgatg atatcgttgg
    38161 tacaacattc aaattagtaa acaacaatac ttactataca aaacttccaa ctgggaattt
    38221 tatccctaat acagattatg atgcgatgta tcagaatgct tctgatgaat tgaagatctc
    38281 ggggatccta cgcgtgaaat cttcttctac aatgaatctc ttatctccag gaatcgctta
    38341 tagcgatcaa ttaacgactc aaatcgtcaa cgaaaacaaa gaatcagaaa tcgtcaaagc
    38401 tcaaagagac agtggtgtaa atgtcttaac tactgaaaaa gttgacgaaa atgccaaaca
    38461 aacattgctt agttatttag gtggagacag cttgccttca agtatcatga tttatccgaa
    38521 caattttgaa gacaaagaaa agatcttgga ttatctagat gattacaata aaggcaaatc
    38581 agatgaagat aaaatcattt atactgattt agctggaacg atgactgaac tgactggcgg
    38641 attgatggat gctatcactt atgtccttat tgcttttgca gggatctctt tagtaacaag
    38701 tatgatcatg atcagtatca ttacttatac atctgtgatt gaacggacaa aagaaatcgg
    38761 tgtactaaaa gctttaggtg cacgtaaaaa agacatcact cgcgtatttg acgcagaaac
    38821 gtgtatttta ggtatttctt ctgggatatt aggtgtgttt attgcatggc tagctacgtt
    38881 ccctatcaat agcatcttat acaacatgac ggatctaaaa aatgttgctc aactaaatcc
    38941 tgtccatgcg attattctag tgatcgtctc tactatattg acgatgcttg gcggtcatct
    39001 tcctgcacga atggcagcga aaaaagacgc cgctatcgct ttgcgtgcag aataaggttg
    39061 tgacaaccac atttagattt gagtcataag agcgctgaaa tagtagaaaa cagcaagaaa
    39121 cgaggaagta atatgttcct tgtttcgcgc tgtttttttt aattattttt tataattctg
    39181 gtatactttt ctttcctttg taccacaact cttctggcgt atacctgtct tttccattta
    39241 tgaatactgt tccatctgct tcaatcgtta cttcagtccc aatccacata ttacgagaaa
    39301 gtgcgagaag attttttctt tttcctcttc ctttttttcc aaattgcaag gcatcggtta
    39361 tctcttctgt ccaatgagat ttcacgtatt ttacttctcc gttttcaaat gtaactttca
    39421 aaatattcgc tatattttcc acttttactg ctttaggaaa tgactcctcc atttttttca
    39481 ctttccttcc tccaacacta agtatgcgct ttcttttagt gataagtata tcatagttta
    39541 ttctattatc aaaaaaacgc atccattcca ttgtagaata ctttaccgaa caagttgaat
    39601 tgttttcctc tttttcacct tttactatag ataggtaaag acaactaagg aggaatctct
    39661 catgacagat caaccgtatg atttaagaaa agtcatcgaa gaactaaaaa cacttccagg
    39721 tcaatatcac gaaacagata ttgaaatcga tcctgaagca gaattatctg gtgtttaccg
    39781 ttatatcggt gctggcggta cagtaaaacg tccgactcaa gaaggaccag ctatgacttt
    39841 caacaatatc aaaggtttcc ccggcacacg tgtgaacatc ggaacaatgg ctagtcgtaa
    39901 acgtgtaggt gaaattttgc accatgatta tcgaacactt ggtcgtttct tgaaagacgc
    39961 tgtagaaaat ccaatcaaac ctgtgaaagt agacaaagca gacgctccag cacaagaagt
    40021 cgttcatcta gctaccgatg cagacttcga tattcgcaac attttgcctg cacctacaaa
    40081 tactcctgaa gatgctggtc catacatcac aatgggtgta gtcttaggat cagatccaga
    40141 aaaaacaatg agcgatgtaa cgatccatcg tatggttcta gaagataaag atacgatcgg
    40201 tatgtacatc atgcctggcg gacgccacat tggtcacttc caaaaacaat tcgaagcctt
    40261 gaataaacca atgcctatca ctatcaatat tggtttagat ccagcaattg ccattggcac
    40321 gactttcgaa ccacctacca ctccacttgg ctacaatgaa ctatggtgtg caggagcttt
    40381 gcgtaacgaa cctattcaaa tcgttgatgg tatagcagtc gatgaagtgg gtattgctcg
    40441 tgctgaattc atcattgaag cagaaatcat gccaaatgaa acaatgcaag aagatatcaa
    40501 cacgaactca ggaaaagcaa tgccagaatt tcctggctat actggggatg ctaatccagc
    40561 tgtacaagta gtgaaagtca aagcagttac tcaccgaaaa gacaatccaa tcatgcaaac
    40621 aacgattggt cccagtgaag aacacgtatc tatggccggt attcctacag aagcaagtat
    40681 tcttgccttg gtcgataaag ctattccagg aaaagtagta aatgtctaca atcctcctgc
    40741 aggtggtggt aaattgatga ccatcatgca aatccgtaaa gaaaacgaag cggatgaagg
    40801 tattcagcgt caagcagcta ttcttgcttt atcagccttc aaagaattga agacagtcat
    40861 tttagtcgat gaagatgttg atattttcga catgaacgat gtagtttgga ctttcaacac
    40921 tcgtttccaa ggagataaag atatcatcgt gatcccagga atgaggaacc atccattgga
    40981 cccatcagaa ttaccagaat ataatcctgg ctctattcgt ttccgtggga taagtgcaaa
    41041 aactatcttt gacggtacag ttcctttcga tatgaaagaa cattttgtac gtgcaaaatt
    41101 ccaagaagta gaagattggg aaaaatattt gaaataatga atgatctgca actgttttga
    41161 catcataaaa aggagtgcgg aaaatggaac aattggcaca acggaagaaa aaaatcgtga
    41221 ttggtgtaag tggcgcttca ggcaccattt atgccatcaa cctagtgaag aaactaaaag
    41281 aatatccgat gatagaaaca cacggaatca tcagtccatg ggcaaaacag aacttaaagc
    41341 tggaatccga cttatcacta actgaattcg aaagcctgtt tgattaccat tattcaaaca
    41401 aagatttagg tgctgctgtc gccagcggat catttttgac cgatggaatg atcatcgtac
    41461 cggcaagtat gaaaacagtc gcaggtatct ctgttggttt aggcgacaac ctgattgcac
    41521 gtgctgctga tgtaacaatg aaggagcaac gaaagttagt cattgttcct cgtgaaacgc
    41581 cattaaatgc cattcacttg gaaaacttga ctaaattagc acgactaggt gtgcaaatca
    41641 taccaccaat tcctgctttt tataatcagc ctcaaacgat ccaagatctt gttaatcacc
    41701 atacaatgaa acttttggat caatttgaaa ttgtaaatga gcattcaaaa cgttggcagg
    41761 gtgattaata tggaaaaaga aacgatagtt gaagtaacca aactaggttt cacgatccaa
    41821 gctgctgtta aagtaatcgg taaagactta ttgatcactt taacaggcgg agatacacca
    41881 catatcggaa cagtcactac attttctcgt gatacagaga tgcaaactgt acgtttccct
    41941 agccacgatg gtcggttcca taaagacgat gtcttagccg aaaaaatagc tgtgatcatc
    42001 aaaactgccg ttccgggaag ttgtacgatc acttccggta tccatgttga ccatatctcc
    42061 aatgcacaaa tcaacgcctc ttttccgatg tcagaagagt taggttacaa gattttagat
    42121 tggttggaaa aaatagattt ccatatcaaa gaaccagttt attataaaga tggtgaaaaa
    42181 ccattatgaa agaagaggtt gtgaaatcag cgttatgacc tgagtatgag agaaaaaaat
    42241 ggaaaaatag cttctcatat tttgccgttt ttgagctaat accgagggac agctgattga
    42301 acgccgttta tcaagaggtt gtgataaagc cgcttcgttt cgagtagtaa gaaaaatttt
    42361 gtaaacatgg gtttccacat ttttgcataa atttgactta ctaccgagaa actggctttt
    42421 gaacaccgtt tatcaagagg ttgtgacacg tcttttgtca caacctcttt tatttttgct
    42481 tcactgtaac tgctacaata tcaccgattt cttttccgat gtttttacga atatcttttc
    42541 gtagtccaag gatgtagcag actgaaccat ctggattttt cacgcccata ttcacgatac
    42601 tgccttcata cggcacattg tcaaaagttg cttggacttt aactcttcca gcaccgaatt
    42661 cttttcttat atcatacgga aaaataatat atgccccgcc cttgccgact tcggaagcat
    42721 agatttttga ttcaaaagaa taaatttccc tcattttatt tctcctattt tttacatcta
    42781 gtatagcata gcaaaaaaaa gaaggaactg tgacaaaagt cttgtcgcag ttcctttttt
    42841 cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatagt caacaaacat
    42901 gagaagctgt tttttgaaat tgctccttgt gactcagagt taaacggcag gctcacaacc
    42961 tcttccttaa acggtgttca aaagcttgca tctacggcac taagcccaat cagatgaaaa
    43021 atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca gctttttcac
    43081 aacctcctac atggtgttca aaatattatt cgccttttgc ttctttcact aattttgcga
    43141 atgcttgttg ttggaattca gcagctaagg ctttcgtacg ttgttcttgc tctgctttgc
    43201 tcatttttgg attttcagtc attgctttta ccataccgtc ttgaacataa gcttttgctt
    43261 cactttccat cgtactttgc tgtttcttca ctaattcagc taacttggct gcttggtccg
    43321 tacttaattc tacccatgtc tctgggattt tgaatgtatt cactcggctg tcatattcgt
    43381 gattgttatc agcaatattg aaccagaatt tatacatgtc gtttttgtat tcccaataag
    43441 tagttttcgt cactttttca gcatcgccgt ctgtcttttc tactttattt gtttcgttat
    43501 ccgtaccagt tgcttttggt ttttcttgtt tttcatttgt tcggtaaaga tacacttttt
    43561 cttttccatt ccctaaagct ttatacaaaa gcatatctgc tccttgggaa tcagcactgg
    43621 aaacaatttt ttctgttttt gtttccgtta ctttttccat tccaaagtga ttggacaaat
    43681 tagcgacgat caaacttagt gaagcgacga agattagtcc gaaaatcaaa gataaaaagg
    43741 tttgccatgt ttttttagcg aacacgttcg ttaaagcaaa agcaagcaag ctgacaatca
    43801 aaactccaat aatcatgcat tatcccccgc tttcattaga acaggttctt ttttcttcaa
    43861 taaaaaggca ataataaagc caagaatccc aaagatgatc gctacgataa acgcggcatg
    43921 gtaacctgac agtgtggcat tgattgcctg atctttataa gctaatggcg tattatccaa
    43981 tacagatttg cttgggatat ttgcttttgt cacattcgtc aacacactga tcaaaatagc
    44041 tgtaccaatc gagcttgcga tttgacggaa tgtattattt accgctgtcc catgactgat
    44101 caagttattt ggcaatgcat tcataccaga agtcgttacc ggcatcatga ccatggagat
    44161 cccaaacata cggatcgcat aaaggatcac gatatataaa actggagtcg tttttgtcaa
    44221 gaaagcaaat ggtgcagttg ccgctgtcaa aatgaacatc cctgtgatgg ctaaacgttt
    44281 cgcgccgtat ttatcgaaaa tcgcacccgt cacaggcatc atagccccca tgatcaatgc
    44341 acccggcaac aacatcaagc cagaatggaa tgctgattct ccacgaatat tttgaatata
    44401 caatggtacg accatttctg cccctaccat cgccatattt gttacaccag ctaaaacagc
    44461 tgcaatcgtg aatgtagatg atttgaatac acgaagttcc aagaatggat gttccaaatg
    44521 taattgacgc catgagaaca aggcgatgaa aatcacacca acaactaaga aaccaattac
    44581 tttcaagctt ccccagccgt catctcctac actagaaaat ccgtaaagta aactaccgaa
    44641 tccaatcgtt gataaaatag cggataaaat atcaatgcta ggatttgaca atggaataac
    44701 acttttcatt aggaagaatg ctaaaaccaa gacaagaaca acgattggca gtaccatacc
    44761 aaacaagtca cgccaatggt agttatcaat gatataacca gataatgtag ggcctaaagc
    44821 aggagcaagt ccaataacga tccctgacat ccccattgct gatcctcttt tttctggcgg
    44881 gaaaatagat agcatgatat tttgcaataa tggcatcgat actcccacac cgcaagcttg
    44941 gatcaaacga ccgattagta atgtactaaa attaggtgct gtaaagcaag taatcgttcc
    45001 gatcaaaaag atcgtcatgg cagaaagata taaatttctt gaactgaatt tattgatcag
    45061 ccatgctgtt attgggatca tgatcccgtt gactagcaag aaaccagtcg ttaaccattg
    45121 cacatcagac gctgaaatat cgaaagcttt catcaatgta ggaaaagctg tcgtcaataa
    45181 ggtctgattc aaaacggtac aaaatgtacc aattaataag acaacaacta aaagtgtccg
    45241 attatatggt tttccgtaaa tatctacggg ttggttgtta ctcatatttc tccttctttc
    45301 tgaatttttt ctgaaactac tagaatactt tagtcctaaa ttcgctattt gtcaacctac
    45361 ttgacacata agttaatacg gatataatta agggaagcaa gaggacaaaa ggagtgacaa
    45421 aaatggttgg ggcttccctt aaacgtttgt tggaaaatga tcaattggtt gttggcatca
    45481 gtgaattaag tgatatcgta ggtgtgtctc cacgtcaatt aaggtattgg gaacaaaagg
    45541 gattcattcg ttcgatcgaa gctgatgcga attgtcctag aaaatatcgt ttacctacag
    45601 tcataaaagt agaactgatc aaaaaatatt tagatgacgg atttactttg tcaaaagctg
    45661 cagaaaaggc agaaatccgc caaaagaaaa tgcaccatgt ccgtaaagtt ttttcaaaag
    45721 caatcaaaga tatcgaattg atcgacgaac gctatacgat tttacaaatc ggtaacttca
    45781 aagaagagaa tgctaagatg tacatcattc atgacaacga gacagacgaa ttaacttaca
    45841 agctccttcc tgtcgatgaa aaagctgact acgaacagtt gttaaaagaa ctctaaacgc
    45901 cactaatctc ctaagtacca agctttttaa taagtagatg ctacataaat acttgaaaaa
    45961 aggcctgttt gaacataaga atatccgaac ggtgatctgc gtttctttca tatagaaagg
    46021 aattctccat cactgttcgg atattttttt atttttttcg gttactttct tctaatacat
    46081 ctgttagcca atttgcatat tctgtttccc gattgatcgc atgcgctaaa atcaggtagt
    46141 gtccgtaatt ttcatgtttt tctttttcag taggaaagat cgtttttttt cgctctaata
    46201 gatgaagtcg tttttcttca tgaaggtcac gctgctgctg aataatttct actaatgccg
    46261 gatcattcac gtctttgacg aaatacaatt tcagaacaaa ttcgtctcga ttaaccggta
    46321 gttcattggt gggtgtatga atccactctg ccaattgttc cttcccttct tctgttaatt
    46381 cataaacttt cttttccaat ttttcacctg tgatctctac ttgatgctga atgattcctt
    46441 gctcttccat ttttgcaagt tcaggataaa tctgactatg ttttgcttgc caaaattctc
    46501 ctatttcatt tttgaacgat tgattcaatt catatcctgt catcttcttc ttacttaata
    46561 accctaacaa aatatatcga agtgtatttt ttcttgccat cagttttccc tccttgacaa
    46621 atctttaaat ccattctatc ataaatgagt aatcgaaaac atgtagttta ttacatgttt
    46681 taatttataa ggagatgctt aaacatgaaa gaatttaaag aattggaaga ttttattgga
    46741 actcacttta tctatacgta tgacaatggt tgggaatatg aatggtatgc caaaaatgac
    46801 catactgttg attaccgtat ccatggcgga atggtcaaag gccgttgggt aaaagaccaa
    46861 gaagctgata tcgttaaatt gacggacggc gtattcaaaa tcacttggac agagccaaca
    46921 gggacagacg tggcattgga ctttatgcca aatgaaaaga aacttcacgg aatgatcttt
    46981 ttccctaaat gggttgaaga acatccagaa atcactgtta ctttccaaaa cgaacatatc
    47041 gcagaaatgg aagcagctcg tgaaaaatat gaaacttatc caaaattact tgttcctgaa
    47101 tttgctacga tcacttatat gggtgatgca ggtatagaca atgaagatgt tattagcgaa
    47161 gaaccatacg atggaatgcc agatgatatc cgtagcggaa aatacttcga tgaaaattat
    47221 aaacgagtaa aataaggttg tgaaagagag gttgtgaaat cagcgttctg acctgagtat
    47281 tagagaaaga aacggaaaaa tagttcctca tgttttgccg ttttttgagc taataccgag
    47341 ggtcagctga ttgaacaccg tttatcaaga ggttgtgaaa tcagcgttat gctttgagta
    47401 ataagaacgc tagttagaaa atagcttttc atattttcta ggctagtggg cttattaccg
    47461 agaagcagcc gtttgaacac cgtttatcaa gaggttgtga aatcagcgtt ttgacctgag
    47521 tattagagaa acaaatggaa aaatagttcc tcatattttg ccgttttttg agctaatacc
    47581 gagggtcagc tgattgaaca ccgtttatca aagattgtaa caactgcata ttcacacagt
    47641 taaattagtg acttcaaaat gaaataatgc tcccagcaaa aaattaggct gggagcatta
    47701 tttcgttatt ctgttcatca aaaatcagcg ttttgattcc atttcatcac aagttcctcc
    47761 tcatttactg cttcatcttt ttgctgtgtt tgaattgtaa aaccttgtga aagatagaaa
    47821 tgaatagctg attgattttt ttgatagact gtcaaagtca gtgttttatt ggtttctttt
    47881 atttttttca ttagttgcgt accgattctt tgttgccggt attccctttt cacaaagatt
    47941 cctgcgataa actgatcaat caccccagca aaaccaataa tttcttgatc tatttctgct
    48001 aagtaaatca ctgcatctgg taaagctttc ctaacagatt ctacttgatt ctcccaataa
    48061 gttgatggaa tgaattcatg agaatccaag ttagcatgca gccaaatcga tagaacagat
    48121 tccagttctt tggcagacaa ttttgttgtt gtgcggatca ttttttctct ccttacttat
    48181 tcattgattt taaaataaaa accatttttt tgaagctctt ttacaagttt tcgcccctca
    48241 aaaaagtata tcttctcttg ttttactttt ctaataccaa aagaaacagc aactttagga
    48301 tcatcagctg aaacgattcg ataagttcca ccaggcaatt gatccagctt gactttccca
    48361 tgatggtcgc tttgtactga aaacggcaca ccatttctag taagtgtttt tttcttatgt
    48421 ttcaaaatga atgaactacc atttaccggt ttccctgacg aatcactaat aataaatgaa
    48481 aaatcaagca cttttttact taatctatac tgatagatta gtcgtccgat aaacactaac
    48541 agtatgacta cagctattat cgttcctgca agaataaacc attgccgcat ggttctttcc
    48601 tgatttcctt ttccttcttc tttttttacc gattcgttat aaggaatccg atgtcctgtc
    48661 actagcaaac gatgcgtgtt gatcatatac ggcgtacatg taagcaaggt aactaagtct
    48721 tctccttctg aaatcgttag aacagacgtg tcatctggca gtaccacttg tatattttta
    48781 acttgataag ccaatttctc ccccaaaaca gtcaaaataa acgtgtctcc gttttctaaa
    48841 cgatctaatt cggtaaataa ccgtcgatta ggtaaaccgc tgtgagcaga gatcactgaa
    48901 tgcgtcgaat ttccaccaag cgggaaagag gttccttgca agacgcctgc tccattttca
    48961 aggatcctac cattgagccg atcataaagt gaaatattta attggatctt aggtatattg
    49021 atacttccga tcagccattc ctctaattgg ctggatgttg tactttcatt tctgttcttt
    49081 tcattgaaag gatcagtacc aggattgaat ccttggtcag ttaatcgttg gtttttctcc
    49141 tccattttct ttcgctgcac ttcaatttca gctgcggtcc gtttttgtac ttgttcgata
    49201 cgtttttgat caataaaact atttaaagaa tccacataaa atggatataa agcgatcagt
    49261 aaccctacga cgatacatat taataaaaac aaacgaaaca aaagtcgacg agactgtttt
    49321 tgcttactct gcttcacatg tgtcctccct tgcaagtttt tttgttcttt cttttattat
    49381 atgggttcag gaaaaaaata gaaaatgaaa gccgatgagg ttgtgaaaaa gctgtcctgc
    49441 atcaagtaat aagtacagcc caaaaaaata gctactcata ttttttgttg ggttgggctt
    49501 attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa aactgcgtta
    49561 tgctttgagt aataagagcg ctagtcagaa aatagcttct catattttct aggctagtga
    49621 gcttattacc gagaagcagc tgattgaaca ccgtttaccg agaggtcatg aaaaagctac
    49681 tttgtttcga gtattaagaa aaattctgtt tcaaaacaag gagatgcaac aaaacttatg
    49741 tcgcatcctc tctccttaga actttcactt atgtttttct tgtaaaattc ataaatattt
    49801 tcatgaattt tctaatttct tgcctacctc tcgcatagga tatatgatga atttatctaa
    49861 agtttgcaaa ggagttcgac aattgaaaaa atatggccca tttttagcaa tttttttcat
    49921 tgctttggca tttctcatgc ctcatttatt cacacataaa ctaattttgg gtgctgatgc
    49981 cccattccat tacaatcggt tctatgatgc cgcgcaacaa attaaatatg gggatttcca
    50041 ttattttcct tccctttatg gattccagca aagtggcagg atcgtgaatg ccgtttacgg
    50101 cccatttttc gcttatttac aaggattgat cgtgttgctt gcaggtagct ggtttcatta
    50161 tcagattctt tcaaatttca ttctttatct tatttcagga ttttctatgc tgattttact
    50221 gagatatgtc aatgttcgaa attgggtaag tgtaccagta gccgttttat tcatgagtac
    50281 gtattctatt caatactggt ctatcaacca aggattcact agctggggga cttgttttta
    50341 tcctttatgt atgatcccta ttgctgattt cgtccttcat aaaaagttcc ctgttatcaa
    50401 agtcgctgtc tcagtgggac ttatgacaca gatccattta ctgtcttcga tcatgttagc
    50461 tttgatgtat atcccttttt atctttatta cttttttaac cagactgaaa aaggaaaagc
    50521 actgcttcaa ctgattaagt cggtcttgct ttattttgtt ctgacagcaa atatttgggc
    50581 aggattagct attgtttaca gtgggaataa gattcttcct ccctttgtta acagagaaat
    50641 gagtcaaaag gcgatagatc tggatggcag ttattggctc cattacccta aggcctttcc
    50701 tatattgata ttatcaggtt ttttgctttt tattttcttt caacaaaaga acaccttatt
    50761 ccaaaaaatc gtgttgtaca caagtcttgt tttctttttt ctttctacta gcttgattcc
    50821 atggacgaca ttgatcgaaa aaaatgtgcc ttttattagt ttgatccagt ttccatttcg
    50881 atttttcgtg ccatttacgc tattgcttct actttacctt gccctttcac tgaaccagtg
    50941 gtctgaacaa aagtggttcc gtcttttaag tatatttggc attcttattt tttcagcaca
    51001 gacgatccag aacttatacc agcatctgga aaagtgggaa aatgaaacat ttgtcagtcg
    51061 acatacttat ctattcgata cgccggaaga agcaaggaaa acattcttca gcaaagatat
    51121 gtctaccagc ttgttcgttt tccaaaaaac cacacctgac tatctgccaa tctataagga
    51181 gacaaaagac aataaatacg accggtataa cgaagaaata ctagaaaacg aaacaacttt
    51241 tataaaaaca catccaggcg gtcttttgac aatcaagtgg aaaaacactg gggacaaaac
    51301 tgttcatatc cctgttatcg tctacgatcg gactgtgcta cagcaaaatg gaaaaacatt
    51361 gactgattat gaagtaacag atatcgggac cccaatcgtg aagcagcaaa aaggaatcaa
    51421 cgagctcacg cttcattatc aaacgccaat ctatttctat ttcattcttt ctcttacttt
    51481 gattggctgg tttaccttgc tatgtttatt tatttatcat agatacaagc ttttacgtgc
    51541 ttaggcaaaa gggataaata catccgatca aaaaactctc caaacggtat aatcgtttgg
    51601 agagttttat tttaatcagt ttttttatgt tatatgttat attttttatt cgttcacatc
    51661 taattcgatt tccaccccgt agtgatcact gatgattgga ctatcgatcc catcaaaaat
    51721 ccgttgatat tttttaacct gcattccttc agggacgaaa ataaaatcta tacgcaattt
    51781 ttctgtattt tgttcccaac catctatttt cttttccaca gtagctgctt ttcctttttc
    51841 attcgcgaca acatacgaat cttgaagaag aaggcaactt tctcttacag tataataacc
    51901 ttgagagtct actggattat tgaaatcacc taggaaaaaa agtggtacgc gagtttccaa
    51961 aaagtagttt tccaagtttt tccactcaaa ataaaagcca ctttttttat ctgtccacca
    52021 agaaaagtgg cagctcgaga caaaaatgtc ctgatcatct atctttgttt tcccaatcaa
    52081 gatcttgcgt gtctgataat cttctttctt ttgaaattgg gagacggtaa ggacctgact
    52141 ttccaatgtt tttttcgtaa gcagtgcatt tccttcttca tatttatcat aaccgatgtg
    52201 gctcatttcc caactccaaa aatactcttt tccgtgttcc tttaaaagct gaactaaacg
    52261 gtaggcaaaa ttatctttac ggataggtac ttgattcttt accggacaga acttcattag
    52321 cttcgcaggt tctacttctt cagattccag taattggttg atttcttgta aagcaatcac
    52381 ttcataatct tctaacagga tcttatctgc taattcttgc aattttttct ctggttctgg
    52441 ctcaagccag ctatgcgtgt tgagtgtcat gaatttcatt ggttttctcc ttgtcttcat
    52501 ttcgaaacaa aagggttgtg acaaaatttt tgtcacaacc tctccagcaa atagtgttct
    52561 cctgctaact cctcagtaat aagctcaatg gaccaggagc tattttttgt ttggctgttt
    52621 ttatcacttg gtgcagggca gctttttcac gacctctcca ctaaacagta ttcaaactgc
    52681 tgcttctcgg taataagccc aaccaaacga aaaataggag gagctatttt tgtttggctg
    52741 ttcttatgac ttgatgcagg acagcttttt cacaacctct ccttacattt taatggagcc
    52801 gatcacttta ccagctgtca ctgtacctaa ttgcttgatc actacttctt ctactttatc
    52861 gtttgtaaat acgatcatga tcgttggatc tttttcttcg ttttggatca catcaagatc
    52921 catctcagct aatttcgttc ctgttcctac ttcttggcct tctttgacag caatctcaaa
    52981 agctggctga ttcatttgga ctgtatcgat tcccatatgg atcaaaactt cgataccatc
    53041 tttcgtttcc agcccaattg catgtttgct agggaaaata cttgtcactt ttccagcgat
    53101 cggcgcaaca acttctcttg atgttggaat aacagcaaag ccgtcaccca tcattttttg
    53161 tgagaatacc ggatctttta cttcagataa aggaatcact tctccagaag ctgcggaaac
    53221 tagttcttgt tctataccta agtattgctt actacctgct tgtccatttc ctgtagaaac
    53281 catttctggc tctggaatct ctgctcctga ttgcagcaga tcttcgatat ctgatttcaa
    53341 gacatctgct tttggtccat aaactgcttg gactccattg tctttgacga tcaatcccat
    53401 cgcccctgct cgtttccagg cttcttcagt ccctactttt tctcgatcat ttacacttac
    53461 gcgtaaccga gtcatacaag catcgacatc tttgatattt tcttttcctc ccagcaaatg
    53521 aatgatctgg accacttgct gatctgctgt tcctgttccg ctgttagata caacggtttc
    53581 ttcactgtca ttgtcgtaat ttccgttacg tcccggtgta gcaaagttga attttttgat
    53641 caagaaattt gctaagaaat acgtaacaac accaaaagcg atcacacaca acacgaagtt
    53701 gataagatct ccacctagac ccgctttgat agctaacggt gtccgagtca gcaattcgat
    53761 attcccaaat gaatggaccc gcaatggcaa gatatctgcc atcgcaaatg cagccccttg
    53821 gatcactgaa taaatgacat ataatggcac agcagcaaac ataaacatga actccaatgg
    53881 ttctgttaca cctgttagaa agacagccaa cgctgctgaa aaatacattg atttatatct
    53941 tgctttctta tcaggatcga cattacgata catcgcaaaa gccattccca tcaagatacc
    54001 tgaagaacca atcatttgtc ctactttgaa acgagccggc gtccagttat gcaacacaaa
    54061 ctcatattgc gacatatccc ctgcaccttt aagattcact aaatcagtgg cccaagccaa
    54121 ccataacgga tcttgcccaa atacttgtgt ccccgcctgt gcgcctgata aaatctcata
    54181 cgatccgcca agctgtgtat agttgatagg aatcgtcagc atatgatgca atccaaatgg
    54241 caatagtaaa cgttctaatg ttccaaataa aaacggtgct agaatcggtg cgctttcttg
    54301 tgattctgca atccataaac caaaattatt gattccagcc tgtacgtttg gccacaccac
    54361 agcaagaatc aatgccacga ttgttgacca taagataaca acaaacggaa caaagcgctt
    54421 tccattgaaa aatgacaaag catccggcaa ttttctataa ttatagtact tattataagc
    54481 cattgccccg acaaatccag cgatgatccc tacgaatact cccatattca aagctggtgc
    54541 ttccaataca cttgtaaaaa atcctttaac catgatcttc gtaccgaaca gagtgtgggt
    54601 aaatgcatct tcattcgcaa gcatgtcact tgtgacacca aagatagccc cagtgatccg
    54661 attgattaaa ataaatgaga tcccagctgc aaatgctccg cctgctcttt cttttgccca
    54721 acttcctcct attgctaaag caaataaaat atgaagatta ccaataattg cccaaccaat
    54781 attttcaatt actccgcccg ttgtgacaag cggcgtccaa tcaggattga tcaagggaat
    54841 tgatttccct atactgatca tcaacccagc tgccggcata actgctacca caaccattaa
    54901 agcctttcca aatttttgcc aaaactcaaa actaaatatc tttttcattc cgtttagctc
    54961 ctttacgcaa tcggtttcgt taaacgtatt atagcgtgcg ttttcttttt tgtaaatgct
    55021 ttatttttta tttttctgtc tataccattc tataaaacct ataatacgtg tgttcccttc
    55081 tataattttc tcctaaaaat ttattttttt aagctttttt ctatgaaata ttttcttttg
    55141 tctattgact tgtgcaatcg gttgcgttta ttatgaacgt ataaaacagc tcattaggag
    55201 gagaaaaatg aaacagatca aacgactatt tgctattgat ccatggaaaa tcagcacgac
    55261 acaattagat aaaaaaaatc ttcgactgca agaatcttta acaagtattg gaaacggtta
    55321 tatggggatg cgcgggaact ttgaagaaac ttactcaggg gaccaccatc aaggtactta
    55381 tttggcgggt gtctggtatc ccgataagac acgtgtcggc tggtggaaaa atggttatcc
    55441 agaatacttt ggaaaagtga tcaacgcctt gaactttatc gcgatagata tttcgatcaa
    55501 cgatcagctg atcgacttgg cttctttgga accagaagac ttttactggg aattagatat
    55561 gaaaaatggt gtattatctc gtacattcac aatcactact tctactaata aagtacgcat
    55621 ttccttcgaa cgttttctga gcattgtcaa aaaagaatca gcttatatct atttaactat
    55681 agaaatgctt gaaggtagcg gaacagtcaa actcgtatca aaattggatg gaaatgttca
    55741 aaacgaagac agcaactatg atgaacattt ttggaatgag attgatcgtg gaactataga
    55801 agaaatcggc tatttgacga ctaaaactat tccaaacaac tttgggattg aacaatttac
    55861 tgttactgca gcaatgcgcc atttcgttaa tggaaaatca gtcatccctg actatcacga
    55921 agatacttta gcattacatg gttgcttgtc tgcagaatta tctcaaggtg agacatggtc
    55981 agtggaaaaa gaaattatcg ttcttactag tagagatatt ccagaagcgc agcaagcaga
    56041 aaaagctgtc tcagaattaa cgaaccagac aaacaattat atccaagcaa aagaagagca
    56101 aacagcggct tgggcagaac gatggaagct tgccgatgtg gtcatcgaag gagacgaaga
    56161 agcacaacaa ggtattcgat tcaatttgtt ccaactattc tctacttact acggcgaaga
    56221 tgaacgattg aatatcggcc ccaaaggatt cacaggagaa aaatacggcg gagccactta
    56281 ctgggataca gaagcgtatg ctgtccctct ttatcttgct ttagccaaac ctgaagtaac
    56341 taagaatctg ctgaaatatc ggcataatca actgccgcaa gcaatccaca acgctcagca
    56401 acaaggatta aaaggcgcgc tttatcctat ggtcaccttc actggtgtcg aatgccataa
    56461 cgagtgggaa atcacattcg aggagattca ccgaaatggc gcgattgcct atgcaatcta
    56521 caattatacg aattacaccg gcgatacgga atatttgaaa aatgaaggac tggaagtttt
    56581 agcagaaatc gcacgcttct gggcagaccg tgtccatttt tcaaaacgag caaataaata
    56641 catgatccac ggtgtgaccg gcccaaacga atatgagaac aatatcaata acaattggta
    56701 tacaaatacg atcgcagctt gggtcttgcg ttacacgcga gaaaattatt taacttatca
    56761 aaaagaagct gctatcacta tcagcgaaga agaactagcc aaatggaaag atatcgcaga
    56821 gaacatgtac ttcccagttg acgaagaatt aggaatcttt gtccagcatg acacattctt
    56881 agataaagat ctcatgcctg tatccgatat tcccacaagc gatcttcctc tgaatcagca
    56941 ttggtcttgg gacaaaatcc ttagatcttg cttcatcaag caggcagatg tactacaggg
    57001 gatttatttc ttcaatgata cttttacaaa agaagaaaag caaaaaaact ttgatttcta
    57061 tgaaccaatg actgtccatg agtcttccct ttcgccaagt atccattcga ttttagcagc
    57121 agaattagga atggaaagca aagctgtaga aatgtaccag cgaacagctc gcttggattt
    57181 ggataattac aataacgaca cagaagacgg cttgcacatc acttccatga ctggtagctg
    57241 gttgtcgatt gtccaaggat ttgctcaaat gaaaactgcc catgatcagt tgagattcgc
    57301 accatttctg ccaaaaactt ggaaagctta ttctttccat atcaattatc gcggacgttt
    57361 attgttcatc tcagtcacag atgaacaagt tactttcgag ttattagaag gttctcctct
    57421 agaaatttac gtctatgaac agtcttattc tttagcggat cgtctagaaa taacgctaag
    57481 aaaggaggaa gcaaatgttt aaaggggtat tgtttgattt agacggtgtc attaccgata
    57541 ctgctgaatt ccattatcgt gcttggcgaa agcttggtca ggaaatcggc attacgatcg
    57601 accacacatt caacgaacag ctaaaaggcg tcagccgaga agactcactc tctttgcttt
    57661 tagcttatgg cggaaaagaa cactcttttt caaaggaaga atttgcagaa ttagcgaaaa
    57721 gaaaaaacga ttattattta gagatgatcc agacgatcga accaaaagat gtcttccccg
    57781 gggtcgtgcc tttactggat tctttaaaag aagaaaaaat caagattgct cttgcttctg
    57841 caagtaaaaa cgggccattc ctacttgaaa aaatgggtct tacaccttac tttgatgcta
    57901 ttgccgaccc ggcagaagca acaaacggaa aacctgcgcc agatatcttc ctccttgcag
    57961 caaaagctgt cggactaaaa gcagaagaat gtatcgggat cgaagacgcg caagccggta
    58021 tccaagccat cctctcaagt ggcgctcaac caattggcgt gggacgtgct gaagaccttg
    58081 gagaggagat cccgcttgtc cctgatacga cttttcttac gatcgattac ttgaaagaaa
    58141 agtggcatga ccatggataa tccagagatc tccgtcgtac cagttccaga gactgaaata
    58201 aagaaaatca cattgaagaa cgactactta gaagtcgttc ttctcaatta cggtgcccgc
    58261 ctgcaccaaa tcttcgcacc agacaaagaa ggaaaatcag aaaatgtttt attgtcttac
    58321 gatcgtttcg aagatgttct aacagacaaa agcttttttg gagcaactgt cggacctgta
    58381 gccggaagga tccgggatgc ttcttggggt gagcatagat tggagaaaaa ctgcggatca
    58441 caccatatcc acggtggcac gaaaggctgg tctttccaat tttgggaagt agaagttttc
    58501 aaaactccat catcaatagg tgtggtcttt tacttaaaag atgaattttc tacctatcca
    58561 ggaccaatca ctgctacgat cacttaccgt ttaacaaaaa acgaattaga gatgacaacc
    58621 tctgctagta gccttgttga aaccatctgc aatccgacca accatgccta cttcaacctt
    58681 tcaggaaatg gaacaagaga catctatgaa catcagttaa cggtcttttt agatggtgtg
    58741 cttgaattag atcaagaaaa actgcctact ggaaattgga caaaaaaaga aaaactgcca
    58801 atcgacttta gaaaatcccc aacactgcaa gagatccttg catgttatcc agatggacta
    58861 gatgatgtat ttcttttgca tcatcctagg ctttccaaaa cttccttaca attattcgag
    58921 aagcattctg gtcgccaaat gactatagca acttctaata aaagtatggt attattttct
    58981 acgactggat tcgaagctga tttctctgtc aatggaaaac agatgcattc aaactacgga
    59041 ttagcaatcg aaccgcaaga aatccctgat atcgttcatt tccccaaatt tggatcgatc
    59101 aacctccatc ctggtcaaga acggatcagt cagacgattt atcggttctc tgcacaataa
    59161 caagagaaag gtgtttttat catggctatt accgtaaaag atgtggcgaa aaaagccggt
    59221 gttgccacct cgaccgtctc tcgtgtcatc aacgatcatc ctagtatttc cgagacaaca
    59281 aagaagaaag tccgaaaaat tatggatgaa ctaggttatg ttcctaacat gactgcccgc
    59341 aatctaggaa aacgcatttc cagtgccatc ggcgtaatct taccccctct tgattcaaaa
    59401 gagcggatcg gtaacccatt ttatcttgag atcatggaag caatcaacga agaagcgcgg
    59461 aattatgata tgactacagc aatcgctact gctaaaaatt ttgaattgct gttagagaac
    59521 gtccagcgta tgcatttaca aaaacaagta gatggtttta ttctagtgta ttcagatagt
    59581 caagatactg tcattgatta tttataccaa aaccacatcc ctttcacttt gatcggacag
    59641 ccttatcatc atgagaacga aatcgtttat gtagataatg ataaccagct tctaggtaaa
    59701 caagcaacag aatttttgat ccagaatgga cataagaata tcctgtttgt gacaaacacg
    59761 actcatgaaa gtttgtattt cgaacgatat ttcggttatc aaaaagcaat gatgatggct
    59821 ggtcttcctg cgttatccag tgtagtacta gagcatccgg aggattatct gtcctttgag
    59881 aaaacgttga aagaaaacga agccacagca ttggtggtca ttgacgatat cttcgccctt
    59941 cgcacgatgc agttggcgca aatgtatggc tatgtcattc cagagacact ctccgtcatt
    60001 agcttcaaca actcgatctt ctcaacatta acacaccctt accttacaag tatcgatatc
    60061 gatatctccg aattaggacg tgtcgctatg caaaagctgc gcgaattgat ccatgaagaa
    60121 ttgagcagtg gcgtacgttt ggtcatccct catcgcttga tcaaaagaga gacagtcgtt
    60181 cctttagaaa acccatacta aatagctgac tcactaaata cgctgaccat ttatcctatg
    60241 gtcagctttt tatgtaaaag aagatactac ttctaacaca ttgtttatct gtaaaaaaga
    60301 gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat aaacggcgga
    60361 agcagaagca actccttcgg aaataagctg aaattcacaa aaatttaaaa agcaattttc
    60421 ttgagtttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt ttttccttcc
    60481 ttcaattttt tacacgtcga cgattcaata aaaaaccgcc taatattgta aagacaggtg
    60541 caagaatcga gaaaaaggcg aatggcaagt aatgcaacgc gctaactttc atcgttccca
    60601 taatgaatgt gccgctgact ccccacggta cgagagaatt gacggtcgca cctgaatccg
    60661 ctagtgttcg tgtcaaatag gtttttgata agccaacgtc atcaaaagaa cgcttgaatg
    60721 tttctcctgg aagaatgatg gacagatact gttctccaac gatcagattg attccgatac
    60781 agctcaaggc agtaaacccg actaaacgag aaggtcggtc catcttttct tttaattctt
    60841 gaacgatggt atcaacgatc agatatttga tcagtaaccc acctaatgcc aatgcaagta
    60901 aaatcaaact agcagaacct aacatactca tgatcccgcc tcgagaaagc agttcatcta
    60961 gctttggatc aggtgtattt gcgacataac cattcatcaa gatgtctgct gcttttccga
    61021 ccgtcaaact tttctcatgg ataaaagaaa gaacgagagc agctgtcgat cccgctagta
    61081 atgtagggat agccggaatt tttttgattg cccccccaaa taaaataact agtggaatca
    61141 atgtccaagg agagatccaa aagctcgctt tcatgttttg gatcatttca ttgatactag
    61201 ctagatcggc tgaatgactg ttatgtccta atatcgtata aaaaatcaac gaaaggacga
    61261 atgctgggaa atccgtataa cgcataccag cgatatgttc gaatagatta actcctccaa
    61321 tacccgctgc caagttggtc gtatctgata aaggcgagca gttattccct aaaaaggcac
    61381 cggaaacgac tgcgcctgta gtcattgcat cattgaatcc aagtaaatga ccgatcccca
    61441 aaaaagcaat tccaatcgtc gaaatcgttg taaatgaact gccgacagtc actccgacaa
    61501 ttgcacaaac aagaaatgct gtcggtaaaa agaaacgcac agaaacaagt ttaaatccat
    61561 aaaccataat cgtagggatg gttccagata aaatccatga agcaactaag acaccaatca
    61621 aaagaaaaat caggattgga atgatccctg gagtgattcc tttgatgatc ccgtcatgga
    61681 tctcatccca tgtgaacttt ttaaatctgc cataaaacaa caaacacatg aaagctgcaa
    61741 ggatcgggat atgtggcgag agctggaatc cgatgatcag tactcctagg agtgccaaca
    61801 acacgatcaa tacagcgata ctttctttta aagaaatatt tttattcatc gtcgatctcc
    61861 tatttcaaat gaaaaggccc tgccatgaaa aaactattca cttggcagga cctcctacta
    61921 aatcagcgac aaaacgtcgg ctgaaagact tattttttta tttttcttct aaactttttt
    61981 ctacctcaat cccccaagaa tctttcttga agtgattgat gatcaatgga ataacgaaca
    62041 tgaccactgt gatagctgct actaataatg catcactcaa tgtcgctgta cctaatgtag
    62101 caaatacgac aacgatgata gagaagatca gcatgcagct gactacccaa gctaatccgt
    62161 ttccgttttt accaattcgg taaggacgtt cgaattctgg atgtttctta cgcattttaa
    62221 gcaatgaaac agcaatcaaa atgtacacga tacaataaat aacagttgta gcatttgtaa
    62281 gtgtcaagaa tacagcactt acgtcatcca ttacaccata aagcaaagca aataatgaaa
    62341 taacgattga ttgtgttaga acgacatttc tagatacgcc gtatttgttt tctttatgga
    62401 atccgaattt tggtggaagg aatccttcac gagctacttg gatgattgtt tttgatggtc
    62461 cagtaaccca agctgataat tgtaaaagca caccaataaa taccatgaag ctgaagatat
    62521 ttccgatcac ttctggtaag cctaggattt gacagtcaat caagataggc tgcgtgatat
    62581 ttgctaattc catttttcca tctggtacga catttgctac aagcatcgca ttgatgacat
    62641 tcaataaaac taatccgatc aaagcgataa atacgccttt tgtataattt tttgttgcgt
    62701 ctttcaaacg aggcatgtaa acagaactca tttcgatccc aacaaaaata aatgtgatac
    62761 cagctaagta tttcaatgaa tcaatatgtt ccaagtcagg gaaagctttt gaccatgaaa
    62821 agtcacctag atagccgttt gagactagtc cgactttgaa ggctgccaaa acacctaaaa
    62881 cgaacatgat gactactggg atatataccc ctagccatac accgatgttc ccgccgactt
    62941 ttgccatatc gaatttcaag ttcaagatcg tgatgatcca gtagataact aaaatacaac
    63001 ctaaaacgaa ccagtgatta ttgccaagtt ctacattacc aaatgtgttt cctaacaatg
    63061 gtcctaaagt agaagcgacc attaccatac ctgggaacat ttgcacccat aatagccatg
    63121 caacgacaaa tccccatttg ctgcccagtg ctgtttttac ccataactga ggaccgcctt
    63181 cttgaggaag catcgtggaa agctcaccgg aaaccattga tatcggaccg gcaaaaaaca
    63241 aaactgcaaa aattgaataa aaaatcaatg tccaaccgac tgcggctaac gtaggaatac
    63301 tccgaaccgt tgcacacagt gccattgtca gaccgataaa ggtcccaaga cccattttac
    63361 ttacttcaga tttactcacc gttgacactc tccttgattt tttggataaa ataacggtgt
    63421 ctttgaagta gtttcagcaa taaacgaaac aattcagcga atatttgagg ggattctttg
    63481 agtttattgc ttgaagggtt tcttcaaaga caacctcaaa acaaagggat cgaagagctt
    63541 aggtctcccc ttgctcttca gatccaaaca tgcgtcagaa acagttagta ttcaagctgt
    63601 tttctaacat gattaccttg cttattttgc ttcgcttgcc aataattggt ctgcgtagat
    63661 tttttccaat ttttcttgaa gagctgcttt gattttttca gcatattctt caaagtgttc
    63721 ttctttgttc atgtatggtg tcattacgct ggcacgcaat acagttactt ttccggcacg
    63781 gttccattct tcatctgaga atcctagttg gttcacaaat tgtaatgggc tgttgccata
    63841 gtctgggata gcaaagtcag tatgtgaagt taagaactca ttaccgtaaa tgcttccttt
    63901 cacgtatgaa gaataatcat atacgtcatg gttcaattta ttcattgcaa ctaagtcatc
    63961 gttgcctttt tctttgaata catagtctac catattgaaa tctggataag ttaatggatg
    64021 aacttcgatt tctttgtctc ctactttaaa tgacaagtca ttcaagaagt tgtagaaacg
    64081 atgtgcacct tcgattgatg cacccattaa tttaccatat cctgtgacat tcaaaggtaa
    64141 gacatggtgt gctgcccaaa cgctggctgc tgttgcccct gcttttgaac cttccaagat
    64201 ataagcccca agtaatgctg ggatatctgc gcctttttca aatacatatg tcgcaaagta
    64261 agagataacg tctctcatgc ggatatcttt gatgacgata ccaccggcag aatatggaac
    64321 atagcccatt ttatgtgggt caatcgttac agattctgct tcttcgattg ctttatatgc
    64381 actgtgtact tcttctaaga tataatcttt gttttctgta aagacattgt atttgtaatg
    64441 aacatctttt aaatcttcaa atggaatgaa gttgttgtct tcgtctaaga aaatcgcacg
    64501 gccgtatccg ccataagcag catctacgtg taagtagaag taaataccgt ctttttctaa
    64561 tacgcgacgc aatgcaacga ttttatcgat accgtcgatt gctccttctt ctgttgatcc
    64621 tacaacaccg actacaccta ggattggtgt tttttcagct gctagaccac gaacgatttt
    64681 ttcaagttcg ttgatgtcca tgcgatagtt gtggtcaact ggcactggaa tcacttggtc
    64741 taaaccgata ccgatgatgt cggcagcttt tagccatgaa tagtgtttag tttgtggaac
    64801 caaccatttt cctaattttt ctaaatgttt tccgctacgt gctgagtgag ctttgatttc
    64861 gtcgattttt tctgggacgc tgtctaacag attcatgatt tcttctgttg acaagttcat
    64921 taattcccaa tcgctttttc cagcaactag ttcaggtgtc acttctttca ttgcaagcgg
    64981 caatgatttg atgttgcggg cataccaaag tccttctaag tttgctaaag aaccatcagc
    65041 aacgatgtgg ccccagccat ctttatagct cattaatttt gcaaattcca ttcctacttc
    65101 ttcttccatt tggcttgttg caggagaaga ttcataggca acgttattac cattccatag
    65161 catagcaaaa ttgtacgcta gtaaagatgg catcaatgtt tcagagttca tatgacccca
    65221 gtaacgtccg gcattatgcc aaggaacgga atgggtacgc atccgcgctg aaatttctga
    65281 tagaacatcc tttgttttat tcacagtgtg ttcaaaacta gcactgcttt tttcttctgg
    65341 cgtaataata ggcatgtctt gaggcatgta gttttgacgc cagcctaaat gttcatccac
    65401 caattcattc aacaaagctt tatagatttg gccgttctca gctttgtccc caatgaatag
    65461 ggcatttagg tttagatctt tcgacaatga ttcactcata gtgaaaccct cctaaaaaaa
    65521 tatttaaatt ttgtcgagcc ctacattaca cctgttcttc tattctgtaa actaagtttt
    65581 ggcctgattt ttaatcatat tttaatatgt atcaattatc cgaattgttc acacttttga
    65641 aataattaaa aaaaacgagg gaatttacaa atgtaaactc cctcgcaaag attgtattaa
    65701 cgacaaccag cgttctattt taggatgggt ttcaaaattg gaatgttgta atagtatttc
    65761 ccttttttaa ggtaaaaact tactaaattc taatcaatta ctttggccaa aaagtaattt
    65821 ttcttacctt ttcgaataac cacaaactta ccatcgaatt catcggatgg atttatgaca
    65881 aaatttaaat cggtaacgcg gtcaccgtta atacttatag ctccgtttga aacatcttca
    65941 cgtgcttgac gtttagatgg ttcaattttt gtggaaacaa gcagttccac gatattttca
    66001 ggtgtgcttg aaatttcaac gttcggcatt ttaccgaatc cttgcgcgat ttcttccgca
    66061 tttagttctt taatatttcc agagaacaat gcttgcgtta ttttttgcgc ttctttcaag
    66121 tcttcttcac tatgcacaaa tcttgtaact tcttctgcca aacggcgttg tgcttcacgt
    66181 ttctcaggtt ctgtctctac ttttttcgct aagtcttcga tttcttcttg agacaagaaa
    66241 gtaaagaatt tcaagtattt cactacatcg cgatcatctt gattcaacca gaattggtag
    66301 aactcaaatg gtgaagtttt cttcggatca agccaaacgg caccgcctgc agtttttccg
    66361 aatttcgttc cgtcagcctt cagcatcaat gggatcgtta aaccgaacgc ttctgcttct
    66421 ggtccttcta attttcggat taggtctaat ccagctgtga tatttcccca ttggtctgct
    66481 ccaccgattt gcaattgcac atcgtatgtt ttatgcagat gcaaaaagtc gatagattgc
    66541 aggatttggt aagtaaactc tgtaaatgag attcctactt ccaaacggct ggcaacgata
    66601 tcttttgcta acatcgtgtt gatgttgaag tttttaccgt agtctctcaa aaattcaagt
    66661 aaagagattt ttgacaacca gtcgtagttg ttcacaaatg tgatatttgc atctttacca
    66721 aacaattttc tcatttgatt ggaaagtgcg tccacgttat gttgcacttg ttccatcgtt
    66781 tgcaacacac gttctgtttt gcggccgctt gggtcgccga tcgttcctgt tccgccgcca
    66841 atcagaatat atggatgatg gcctgccaat tggaatcttt tcatcatcat aaatggaatc
    66901 aaatgtccga tatgcatcga atcaccagtt ggatcaacgc cgcaataaag tgaaatgctc
    66961 ttttcttcaa cgagctcgcg aagtctttct tcgtttgttt gctgattaat ggcgtcgcgc
    67021 caagttaatt catcaataat attcatttta ctgacctccg tgtagttttt ttatgaaagc
    67081 aggaacctgc tagcatatgt aattaattat ttcttcagga taggatagga aataaaaaaa
    67141 gtcccttggc atataagcca agggacgaaa tctcgcggta ccacccaatt tgtatgtcta
    67201 gatatccaac atacctctcg aacatttaac gctgtcgcgt ggcaatcact gccaaagact
    67261 cctgaaatgt aattcgcttt aaagcatgta ctggtttgca gcgaccacca gctttctgaa
    67321 acattggctt taacaactac ttcgtttcga tcttcgtctt gttattatta atatgtgtct
    67381 atcatagagc gcaacttttt ctttgtcaac agaaaaagga tatttttttt aaattcctca
    67441 ttttttcttt tttgaatccg ctctaattcc gaacatttta ttttcaatta ttgttaaaag
    67501 aaataacttt ttctgtattt tttctgcaat ttatcgagaa gtcactttac tttgcctctt
    67561 attctttctt cgttacaata aggaatggga caagtaaaaa aatgaaaaag caagggacga
    67621 ggtatatgga aaaaaagcaa aaaattattt taggagccac ttctgcttta actatcgtta
    67681 ttttaattct aagtttgttt tttgtcatcc gatcatctac aaaaaaagaa ccaatcattc
    67741 ctgaaaaaaa aataacagaa aaagcaaaag aaccgacaga tgataagcaa caactagtta
    67801 ctcaagcaaa aaatctggct caaagctacg attatgacca agcgattgcg ttattggaaa
    67861 aagatagtaa gaacggatct actgaaacac aacagcttct tcaaacacta aaaaaggaga
    67921 aagaacaact gatcgactgg tctgatccta ctcagatctc tcatgtcttt ttccacagct
    67981 tgatcgtgga cccgcaaaaa gccttccact ctcaacaggc acaaggatac aaagattata
    68041 tggtgactgt tgaagaattc aaccgttcga tcgaacaact ttatcaaaat gattttgttt
    68101 tagttaattt gaatgatttg atccaaaaag acgaacaagg aaatctttcc tttaacgggc
    68161 ttgcgctacc tgaaggaaaa aaaccattgg ttctttcaca agatgatgtc agctattatg
    68221 aatatatgga taattccggt tttcctagcc gattggtcgt atcaaataac aatgaaatca
    68281 aaaatgtcta taaagagaaa gataaagaag aaatcggtga ctttgacatg gttcctctga
    68341 tcgatgcctt tgtcaagaaa catcctgatt tttcttatcg aggcgcaaaa ggtaccttgg
    68401 ccctgacagg ttacaacgga gttttaggtt atcgaacatc aaaatctcaa tatggagaca
    68461 atgaaaaaac aaaaaaagag attaaagaag ctaaaaaagt ggcggaacag ttgaagaaag
    68521 acggctggag ctttgcttcc catacatggg gtcacttgaa tatgactcaa gcttcactag
    68581 aagatgtaaa aaaagacaat gaattgtggc agcaagaagt tgcacctatc ctaggaaaaa
    68641 caaatattct gatttatcca tttggtgcag acatcagcga ctggcagcct tattcagcag
    68701 aaaatcaaaa atttgcttac ttgaagagtc aaggtttcga tattttctgt aatgtcgatg
    68761 cttctacacc agcttgggga caattaggta cggatttcta tcgaaatgct cggatcaata
    68821 tcgatggcat tcgttttcaa tctgatttag acggaaaaaa tcctattctt gatccattca
    68881 tcaatgtcca tgatgtctat gacaaagaag cacgcgaatc tgcttgattt tacgtcattt
    68941 atgaaccaaa aaagccgaac tgacatttta tcgtcagttt cggctttttt atgctttatt
    69001 tcttcttcgg tgcgattccc ttgatcagga cttctgtcca ttctgctgta tatggaagca
    69061 gttttgcgta tatctccggc ttagaaattc cttctaccat tgcttgaaaa tagaggatca
    69121 agaactcatc cgaatatttc aaatccactt ttccttcttt ccgccctcga tggaaaagat
    69181 caagcatgat actgaaactt tcttgcgtat acgcatccat cgcgtcagaa aattcttttt
    69241 tttctttttt tgtatagtat gtcatcaaat cgatataaaa ttgtttgttc actcgattga
    69301 gataacggat tttgttttca ctcatggcaa tcagtgtttc ttcaaatgga agattttccg
    69361 caatcaacgc tttcgcttct tgccctaaat cattcataaa tgcaaaaaat acttcataaa
    69421 tcagcccttc cttgctgcca aaatatttaa aaatcgtcgc tttacttacc tgagcgttct
    69481 cagctacctt atccattgtc actccagcgg ttcctatgtc tgtgttcatc aagtcaaaag
    69541 ctgcttgtaa aatctctttc tttttctctt gtgctctctt ttcaaaaccg ttcatttgta
    69601 gagcctcccg taattttaaa cttttataat atgtatagtt caaatttatt ctgaggttta
    69661 ttataactat ctattgagaa agtatcaagc tagcaattcc ttgtttatga attcattgac
    69721 agaaatttat aaaaagaata ttatgtacta aaataataaa aaaagtttta taaaaaggta
    69781 taaaaccttt ttattaaact atttaatctt gtttagtata tatttttaga aaaaggagtg
    69841 gggtttttat ggcagaaatc gtcaaagtca cacatttgaa aaagagcttt ggtcgtttcg
    69901 aagctttaaa agatgtctct ttcaccgtac attcaggtga agtcgttgga tttatcggac
    69961 caaatggttc tggaaaatcc acgacgattc ggacactttt agggattata aaaaaagacg
    70021 gcggaacggc aacgattttt gggcaagatg tatgggaaga tagtttatct atccatgaac
    70081 gtctttctta tgttccaggc gatatctctt tgtggggaaa tttgactgga ggagcgatca
    70141 tcgatctctt catgaagctt catggaaaag ggagtgctca aaaaaaagat gaactgattc
    70201 aacgtttctc tcttgatcct aagaaaaaag caaaaaacta ttcaaaagga aatcgccaaa
    70261 aagtgggctt gattgctgct ttggctgttg attcggatct ctatttattt gatgagccta
    70321 cttctggttt agatcctttg atggaaacga ttttccaaga agaagttgaa aaattgaaag
    70381 aacagggcaa atccatccta ctttcttctc atattctcag tgaagtcgag cgactagcgg
    70441 ataaagtcgt gatcatacgc gaaggaacac tcgatgaaga aggaacactc gatgaattgc
    70501 gtcatttgac tcgttcgacc atcactatag aaacagaagc agagaatagt cacttagcac
    70561 aattgaacgg tgtccatgac tttgtgcaaa aaggtcacct cgctcaattt gctgctgata
    70621 acgaagcttt cgatgtgatt ttagctgaag cgtccaaatt aggtgtgaaa aaatttgaat
    70681 ctattccacc gactcttgag gatttattca tgagacatta tgaaggatga ggaggaagcg
    70741 aaatgactga taaattttca cgctggtcca cccttttttc tcaaaacctt agccgtgact
    70801 ggaaaaagat cctgatatgg ctagtcggtg tcactgcttt ttctggtggt tttgtccctg
    70861 cgtttgaaga attgacttcc ggacaaggat tagctggatt atacgaaacc ttgcaaaacc
    70921 cggcaatgat tgcaatggtc ggaccaactc cagcttcgaa tgctgctgag tatactttag
    70981 gagcgatgta tgcaaatgaa atgttgcttt tttgcagttt gtttgctgct gtgatctctg
    71041 ttttacacgt ggtgagccaa acgagaaaaa aagaagaact tggtctgatt gaacttattc
    71101 gttcctttcg agtcggacgt caagcaaact ctttagctgt catcgctgaa agtattctga
    71161 tcaatttgct tttagcaata tcgatcagct gtacgatgat ttttttccaa gcggattcga
    71221 tcactgcaga aggtgcttgg ctttttgggc tatctgtagg tagtatgggg attttgggga
    71281 gtgccgttgc actgtttgct gcacaactga tgccaagttc agccagttcg aatggtctgt
    71341 ctttagcgtt tttaggtgga ctttaccttc tacgagcaat aacagatgta gctgattcct
    71401 ccctttcgat gtggaatcct ttaggctggt cttatttgac ctatccattt acaactaaca
    71461 actggattcc tttgctttat ttgtttttgt tcagtctaat cttgataatc agttcatttg
    71521 ttttagagga acatcgagat ttgaacaccg gctatatccc cgaacgtgaa ggaaaaccgt
    71581 atgctaaacg ttctctttta tccttgcgtg gtttattatt ctatctaaat aaagggatga
    71641 caatcagttg gcttatcact tttggtttga tgggggcggc atacggctct atctacggga
    71701 atatgcaaca gtttttagaa agtaacgact taatgaaaca gatgtttatc caatctggcg
    71761 tctcaattga aaaaaacttt acaagcacga tcatggtagt cttgatttgt ctagctgcta
    71821 ttttaccaat tgctagcatc aatcgtttat ttacagaaga aacccacgga cgtatgaatc
    71881 agctgttcgc aacaaaaatt tctaggaacc aactttattg gaccaatatg ttactagcta
    71941 ttctatttgc tatattggca atcctgatta gttctggtgc actagggctt tctgctttgg
    72001 cttcgatgaa ggatgcgaaa gacatggatc tattgatgtt tcttaaagcc gggttcaatt
    72061 tcctgcctgc cgttttagtg tttatcggaa ttagtggctt tctattcgga tggcttcctc
    72121 gaatcggtaa acttgcttat atctacttag cttactcctt tgcactcaat tactttggcg
    72181 gaattttgga tttaccagaa tggttttcca aaacagctgt ccctagctgg gttccacgga
    72241 tgccaaacga ttcttttgat ctttccgtgt tccttacgtt atcatgtatc agccttgcac
    72301 tgttcgtcct tggttctatt ggttataggc gaagagactt actagaagga taataaaaaa
    72361 gcatgtacaa tttcatgcta gcctccaaat ctgggtcaaa aatccaacat ttggaggtca
    72421 tttttttatt tacaagtctt ctaactgctc tttatcaatg atccagactt ctcgattctt
    72481 tctttttaca taaccattct cctctaataa tttaaatttt ctagataaag tttcgggggt
    72541 cgtaccgata aatgcagcaa gttctttcat tttcattggc aggaaaaagt gatccttccc
    72601 tgtcgcttta gctaagtcaa gcagatagac agcgactcgt tcttctactt tttccatcat
    72661 caaaaaagtt gtctgctgct gtacttttgt taatttctca acgttgattg ctaaaagttt
    72721 cagactaagg gcgggataat gcagtaagag ttccgagaaa tctttttgtc tcaatatgca
    72781 aatttccgtt ttttctagtg cttcgccaaa taaagactca tttggaacgc ctagcaactg
    72841 gctctctccc tcatatccgc ccggctctat tactcgtaaa agctgttcct ttccgctagc
    72901 agataattga tacactttca tactgccgga ggccacgatc actaattgag gttctccatt
    72961 cggcgcaaga atctgttctc ctttttccac atagtgatgt ttgacaagtg cgtgtacttt
    73021 ttcttgatct tgctgtacta agtgattgaa caaaggaaca aatgaaacac ataaatgatc
    73081 atgactcata atctctcctc cttctaaaca gctatttcta ataaagaaac ataacttccc
    73141 ttattccaac tgataatttt cttttatcat agcataaacg tcaagcgaac cagataccag
    73201 caaatagagt ggatttcttt ttgtcccttg ctttattttc cttcttttga gaccttaagt
    73261 aattgattcc tatcaatttt ttaaatattt actttttgaa taatcaaaaa caaaccttca
    73321 ctcgcaagcc gagagtaagg tttgtttttc gcattgctta tttcgtgttg tctattgaaa
    73381 tttttccgca cctagctttt gatatagtgg tgctagttct tgtaaggttt cagcggttat
    73441 ttgctcaacg tgttcgattt cttgcggata gaagaatagt ttacccattt gaaaggttcg
    73501 gtctctcatc gcttttttca actcttcttg tttctttgta tcggacaaat agtcagacaa
    73561 agctaagtgg tcgtcaagtt catgttctgg ttgcggccat atgtaataat ccttgatatc
    73621 gactgttttg ctccactctt tcagtatcgc aagcaactgg ttatacgctg caatgctacc
    73681 atatcgttct tcgcttttat aatgttggta catcaaatag atttggtatt gtttttgatt
    73741 caacaaaact gctagacagg cattctcgtc ttgacgagat tcgcttcggt acgcgctcca
    73801 aaaatgactg cgtaaattcc agccattcgt ccagctttcg attttaggtt tgctcatgcc
    73861 gtacatatca ggcaaaagtg cagctgtctg gagctgaatc tctttccatt tttgccagtg
    73921 ttctttatat tctttcttga tttgttcaat cttatctgtt ggatggctct ctttgagctg
    73981 tttgaatgcg tagattggtc gatcaaacaa ctgataatct gattttccaa gttccattgt
    74041 ttcgcctctt tccgttatct tttgtattct acctgaattt tgcgatatgg acaaatagag
    74101 gttgtgaaaa agctgttctg catcaagtca taagaacagc caaacaaaaa actgcgatga
    74161 gcatcaccgc agttttattg aacttaaggt tgattgatca catctaaaag cgttgaaaat
    74221 aaaatggcac tagaagcagc tcctgggtcg atatgtccga ttgcacgctc tcctagatag
    74281 gaagctcgtc cttttttagc taccaagact ttggtgtgtt cttttgcctg gtcaatgatt
    74341 tcttttgtta aattccctgc cttcaatgcc tctgtgactg gcagccaaac atctaacatc
    74401 gttttatcgt cagcttctgc ctttcctcgg ctttgaatac ccgctgttcc ctgctcgatc
    74461 acttcgccta atttttcttg agaatcaata gaatcgacct cttttgtcgc tttcatcatg
    74521 ttcatgaatg cagatccgta taacggacca gaagccccac cgactttcga tatcatcgcc
    74581 atagagaata ctttgaatgt atcgctgatt gtttcaggat gatcggcttg aaaagcttct
    74641 tcataagctg aaacccctct tcccatgtta tttccatggt ctccatcgcc aatcggggta
    74701 tctagatcac tgagatattg tttgttctga ttgattgcct ctgcaaattg cgtgagccat
    74761 tcttggattt cttgaacggt tagtttcata ttatcaccct tttatcttga gattttttac
    74821 catgaaatcg tctgtacatt gctttctaaa gaatctttcc attgtgtctc cgtcaagtcg
    74881 atcagtgtta gagaaagacc ttgcatatct agtgaggtca taaaattacc gacttttcgg
    74941 aaaactacat tgacgcctct ttcttccaac agagctaaaa catcattcat gaacacgaac
    75001 tgttccatca aaggcgtccc gcccattccg ttgactagta cacctgcttc ttttggtaaa
    75061 ttcgagtaat catctaaaat cttagtaaca agttcctttg ccagtacttt agaaggctgc
    75121 atcttttctc gacgatagcc tggctcaccg tggataccaa caccatactc gatctcatct
    75181 tctcctagtt cgaatcctgg tttccctact tctggtacag ttgctgctcg aagcgctaga
    75241 ccgatcgtct tagttgcctg tacgaccttt tctcccagtt ctttcaactc agctagggaa
    75301 gcaccgtttc ttgctgcatc gcccacgatt ttgtgtacaa ggacagtacc tgcaactccg
    75361 cgttttccag cagtatacgt actattttca acggcgatat catcatctac gatcacactt
    75421 tctacttcaa tatcgtccat agcagctaat tcctttgcca tatcgaagtt caatgcatcc
    75481 cctgtataat tcttaacgat cagcaaaact ccttttccac tatcggcttc cttaatagcc
    75541 gtctgaatct gatctggagt tggagaagta aacacatcac caagcaccgc tgcacttaac
    75601 atcccttccc ctacaaaccc ggcatgggat ggttcatgtc cgcttcctcc accagaaacc
    75661 aaaccaactt gttccgcttt ctgattcttg gctacaacac gggaagattc tacacgatga
    75721 acaagttctg gatagctttt tactaatcct tccagcattt cttcgacaat attgcttgga
    75781 tcattcatga tttttttcaa tttattccca tcctttcttt ccggcatttt gtgcttctgt
    75841 taagatttgc tgagtatcat ctgttatacc ggcagtgatg gctgctgcaa aagctccttc
    75901 cactaaaggc gcatccacca gatgataacg ttgctgctgc tcttcttcca acatgtcttt
    75961 tgccagttcc gagttcaata ccgcgctacc gatatccgca aaaacaaaat aactagctgc
    76021 atcgcttgat tcattgactg cttctacgat tttcatcggg tctgatccga tctcgccatt
    76081 tattccgccg agagaaaaaa tttgcacctc ttcggatttt tgcatctgct caatcatttc
    76141 tttaatacca tctgttactt tttgactatg agaaataagc aaaatacttt ttttcacaag
    76201 aatcttcctt tcttttacat agttgctaac gattggtttc gctttcatta atgaacaatt
    76261 caagtatata ataaatttct aattttggga aaatatgtgt agtgcttctt gaagtttgtt
    76321 atactttaat taaaagagag gagtgatcga atgattacaa caacaacgaa cagtgtagag
    76381 ggaaaagccg tagtagctta taaagggatc gtttttggtg aggtcattac tgggatccat
    76441 gcatttaaag acttggaggc ggggttgcga aatatctttg gcggccgctc aaaaagctat
    76501 gaaaacgaac taatgggtgc aagagaagaa gcacttgccg aaatggccga acgtgcaaaa
    76561 gcattaggag ctgatgcgat cattggcgta aaaatggact atgaagtatt aggcagcgac
    76621 aatggcatgc tgatggtcac atgtagtgga acagctgtct ccacgagata agtaggggac
    76681 atctgtttta ataactaaag agagatggga catgtagtgg cactcaagaa ttagattttt
    76741 cagtctaact tcgaggtact ctaccttccc atcctctctt ttttatttct ttttgcggat
    76801 gattccgtaa gctgctgcga ctaacacacc cgcccctgct aaggaataac tgacagaagc
    76861 tgttttttca tttgtttttg gcaaagtctg acttgttgat ttggatggct ctgtcgtttg
    76921 ctgaactgtt tctgtcgtgc ttccagaggt cactgatttt tctcctgccg aatgcaaaat
    76981 atcttttatt cgttggaaac tagctgctaa gtcagctttt tttgcttcaa aatcattagc
    77041 aactgtttgt gcttcgctga tctttccgtg gattgcttga ctggccgctg tgatatccgc
    77101 ttgagtttga cttagctttc cagaagctgt ttgagcaacc tgatttaatt gctgattgaa
    77161 ttcttttgct tgttggatct tcccgtcaag atctgtttgg acagcttccg cttttgcttt
    77221 cgcttcggca agctttttct caagttgtgc acggtcattt cccactttat ttttaagatc
    77281 agctaaatct tgatcgattg ctgcttggtc ttctttcaat ttgttttttg cagcttctaa
    77341 tgcttcttcg agttcttttc ttttattttc caagtcttgc aaatgatcga tttggtcaga
    77401 agaggtagaa ctatttattg atgtatcttc tgaagatgaa atcgtttctc ctgaattatc
    77461 tgtcggtaca ttggaagtca tttctgtttc cgaggcaccg ttttcagcac cagaagagct
    77521 tatggttgtt gaaggttctg tttgagcagg ttcagaagtt tccgcaaaag ctgtctgtcc
    77581 caataagaaa atactggaaa cagcaattgg ccagattaac ggcttcattc tttttttcat
    77641 cacttttccc atccttacta ctttttaact aatttcttat tataagcaaa aatgtttgga
    77701 agaaccataa acacaacagg ctgtttgtct aaaaaacggt gcatctttta tttttttgta
    77761 cgttccgggc ttttccatgt cccgcttcca taacctaaaa tcgttttgac tgctggaatg
    77821 aaagtcgtca cgatcgttat cgcgttcact atccagaaca aaagcatata tagtggtgcg
    77881 aaaagaaggt atttcaattt tcttcgtcga tcatctacaa tcagcgaagc tagcaactgg
    77941 aaaaatcctg cgaccatttc aaagcagatg aacacaaaac tgaatgtaaa agtaatataa
    78001 atctgttcgt agtttccttg atatacataa tgcccgatta atccagcaaa aagaactacg
    78061 gaaatacaaa agaatattga ccagacaata ctcaatgttt gatcaatgaa gatgatcgtt
    78121 cttccaatat gtttaaaagg atggagcatc acttttttga aattcgtcag ccagacttct
    78181 gttccacctt ttgcccaacg ttttctgtgt cgatatagca tcttcaacgt cacaggtact
    78241 tccatgaaaa agataatctc aggagcaaaa acagataacc agtcatttaa ctgatgatcc
    78301 caagcaatac tgatgtcttc tgtcgctcga tcttgtctga atccgcccac atcgatcaag
    78361 gcttcttttc gatacatcgt atttgctcca ctgtacgcat aaagaccacc gaaaacagct
    78421 gactgtgttc gtttaatgat gcccacgata cttgagaatt caaccgtttg agattttgca
    78481 atcaatttgg tacgattttg gacatccata tttgcagtga ccgcagcaat atgtcttgca
    78541 ccagggcgaa tgaaataatt cacataccta atcagtgcat ctggctccgg taccgtgtct
    78601 gcatcattgc ttaaaatcaa tttccctttt gcaaaggcaa gtccgatatt aaacgcgtgg
    78661 gcttttcctt tatttttttc gatccgaacc acacgaagat tagcgtattt tttcatcagt
    78721 ctagctaata tttctggtgt ttggtccgtt gaaccatcgt ctgtcactaa tacctcatag
    78781 ttgtgataat tcaatttcgt catcaaatac tcgattgtat attcgatcac aatttcttca
    78841 ttatgggctg gcaccatgat cgtgatgaac ggttcgacac tcaagggaac atctacccat
    78901 tctttttgct tatgtttgta taaaaacaca taacaccaga caccaataaa ccatacaaat
    78961 ccgcctaaaa tcggataaga aacgagaaaa aaatacataa tagacaaaca agtatctaag
    79021 aataaattac ctgtatgggt gattgagatc atttcagttc caccccattt tctttataca
    79081 aatctgcaat gaatgtcgta tctagatttt tctcttcgga gacagaataa aaacggaccg
    79141 tttctctctc ttcttttgtt ccaaaacgtt ccgtatacac ttcttcaagt aattcttgtc
    79201 tttttttcaa tttttcatct tcatattcaa aagatttatt tacttttttg tatagatttc
    79261 gattgttcca cctagtcaat acaaatgagg taatcaggaa actgaaaaaa atcaatacaa
    79321 aaaagccgat taagaaatac agcattttct tttctgataa aaaagcgtac aaggaaaaat
    79381 ccttggctct ttctggtctc gttaatggga aaaagaccca tataaaagga agaacaacga
    79441 tcatccatcc taaaagagcc actaacgttt gccaaatctt taaccagtaa ttaccttttg
    79501 aaaaatactt atctttcacc aaaattgatg caacttcttc tcttgttact tttttcatat
    79561 cctcacctca cgtttttttg ttacgacgta ggagtttacc atacatttca tttcttttca
    79621 cataaagtgt tcctaaattt ttcctaagaa aaagaacaaa acatttctca tcagcaaatg
    79681 aaaaatattt tgttcttttt atcagttgtt ctttttattt tttatctctt aattatgttg
    79741 ctctttccaa tacgacaatg cttttcattc ctctagatat cgccgtgtac atttgtctgc
    79801 gtcttctttg ctcatctcca tcgttttcaa atggatataa gattacatga tcaaattcca
    79861 gtccttttgc tagactcaaa ggaatgattt ggcagttttc taatccatat gaattttttt
    79921 taatcgattc ttgtagcgtt tgggtttcct cttctgtttg ggtgataact gccgcttttt
    79981 ctccttttag agaaaccaaa atctcagcta gtattttcaa caattcttca gaggatcgaa
    80041 aagagcggta ttcgggttct tttccttgcg gtcggatcga tacgatgtcg atcttctctt
    80101 gatccacagc ataggttttg aaaagctctg tgattgctcc gctagaacgg taactatttc
    80161 taagatcata ccgatggatc ggcagatggt agtcgtcgaa acaacgcata atatcagcaa
    80221 aagtagtgga agaattaaag attgcctgat tctcgtcacc tactaaagta aagtgagttt
    80281 ttggaaataa ttcgatcaat agacctaatt gtgccagggt ataatcttgt acttcatcaa
    80341 tcaatagata gcgaaatgct ggtacattta ccttttcaac aagtaaatga cgaatagcta
    80401 atataatgac tgcttcatca acagtacgtg tgggttgcca agcataagct gagccatatc
    80461 gtttttcata aattctttca aacaactgat gctcatccac ccatgccatt tcttcgattt
    80521 gtctggttat ttttcgatat tttttttgta aaagtttcct tgcataagct acaatacttt
    80581 gttccgaatc atcagaaatc aatttaccga ataattccaa ctgcctatct tctgtcaacg
    80641 agagtacctg attctgcaca gcaggacttt ttgcttgttt caaaagatga ttcttccatt
    80701 cgctcatcag tgcctttttc gttgcttgaa gcctatcgat caatttagga tgagcaggaa
    80761 ctttctcata tattttttcg atcgtttttt tagaaaggac tgtttttcca tttttagtaa
    80821 gtccacggat aaaattcgga tgatctaaaa acaaaggatc tgattgtttt agattatcga
    80881 taaacttttt gctacgcaaa cgttctgtct gctccgatac attttctccc gtgatacgtt
    80941 tgaaataagc ctcttcacct tctatttcct ctgctgaaag ctgtgaaacc agctgtatga
    81001 tggttagatt cagtggattt ctttcaccta atgatggtag gacattcgaa atatagtcga
    81061 taaaccgatg attaggtgat agaatcagca agtcatcgga tgtcatcgta tctcgatata
    81121 aatagagcag atacgcaata cgctgcatga tagcagaggt tttaccactt cctgctacgc
    81181 catttaccaa gatattttga cttttcacat cccgaatgat tgtgttttgt tccgattgga
    81241 tcgtagcagt gatagccttc atttcattgg tctcatcttg gcctaagact tctaataata
    81301 cgtcatcttg aatagctaca gacgtatcaa aaaacttgac caaacgatct ctttccaaaa
    81361 gaaattgtcg tctttcttta ataaccgtat cgattttatt ttgacgaacc gtataagaag
    81421 atggaccaat catgttattg taaaaaagtt cggcaattgg tgaacgccaa tcataaacaa
    81481 gattttcatc tgcatcatct gtgaaatttg ctgtaccgat ataaaaggat tcttttggct
    81541 cattatccag aaagtctaca acgacttttc caaagtaggg acttttcaat aaacgttcga
    81601 tctttgctag ctgattttct gcgtttttct gtttgatatt catctgatcg atttctctat
    81661 ttttcatttc aatcatcgaa aaagtatcca ggttatctaa gtaactgtca aaattcaatc
    81721 gaagctcgcc agccatttct ttcaacgact ggtttccttc tatttttgcc gtatccatcg
    81781 attcccgcag ttcgtttttt ttatcaatta gcttttgata cacgttcttc agatatgctt
    81841 cttctaactg tttttgtgtg ttgaccatac acattctcct ctttttttaa agtggtggaa
    81901 ccgattagtt tacctctttt aagagtcgaa tgcaagcttt ttaacgagta tcatttgtta
    81961 ggatatatca gttcttcagg tcggacagtt tgatataaag aaaatgcacc atcaaggttt
    82021 tttacgtggt agccagactg cttcaagatt ctttccgcta gatagctacg taatccgcta
    82081 tgacagctga cgatatattc ttgttggctg tctaattcat ttaatcggtc ccgaagttca
    82141 ttcaaaggga tatgtttggc ttccttgaat ttgccctttt ccaattcttc tgctgttcgc
    82201 acatctaata aaatttttcc ttttgctagt tctgtcggca attcatgcca ttgtacggtt
    82261 tcactaagcc cttctgcaat gttcattgct gcatatccta gcatgtttac tggatctttg
    82321 gctgagccaa atggcggtgc atacgtcaat tctaattccg gtaaatcgaa gatcgtcaaa
    82381 tgccctttga ttgctgttgc taaaatatcg atccgtttat ccacaccttt tgccccaaca
    82441 ccttgtgcac cataaatctc gccagtctct ggatggaaga tcaacttcag taaaatatct
    82501 gttgcttctg gataatagct tgcatgatct tttcctgata catgaatgac ttcaaaagac
    82561 agttcgtttt ctttggctgt tctttcactt aaaccggtgg atgcggcagt cattccaaac
    82621 gttcgtataa ttgctgttcc aataccgcct tgatttcttc gagaaattcc ggaaatcgtg
    82681 tccgcaactt gtctaccttg acgattggct ggtgaagcaa gagaaataag tgcatctttt
    82741 ccagtgattt gctgttttac aacgatagca tcccccaccg cataaatatc aggaatattc
    82801 gtttgataac gttcgtctac taggataccc ccgcgtatgc ctaattcgat tccagcggtc
    82861 actgctagct ggttatcagg acgaacacca atggataaaa taactaaatc agcagggatg
    82921 gtacgcccat catccagtcg caatcgtttc ccgtggtctt caaatccagc gacagcatga
    82981 gaagtaatca cttctactcc ttgatgagac aattcttttt ctataaaagc tgccatttct
    83041 tcatccagtg ttggaagaat atgtggtgct tgttcgatca ccatgacttc taaacctctt
    83101 ctttttaggt tttctgccat ttccaatccg atgaatcctg caccaacgat cacggcacgc
    83161 tttggctgtt tttctaatgc atgcatcact ttatcgatat ctggtacatt tctaatagaa
    83221 aaaacattgt cggcttctgt tatccctgga atcgatggaa caactggttt tgcaccagga
    83281 gataaaacca atgcatcgta ctgttcaatg tgtttttgac cctcatgtac gacttctacc
    83341 gttttgtttt cgggaaagat ggctgtcact tcatgatttg gacgcacatc taaacaaaaa
    83401 cgagcggata atgattctgg cgtttggaca agaagatttt cccgttcact gatttctccc
    83461 gaaagataat aaggcaagcc gcaatttgca aaagaaacat acggtccttt ttcaaaaaca
    83521 acgatttctg catcttccat taatcggcga agccgtgtag cagcagacat tccacctgct
    83581 acacctccga caataacgat tttcatctta tttccctcct actgttcgat caaaccattg
    83641 attcatgcca ccacgtacat tcactacatc ataaccagat ttctttaatt ctttagctgc
    83701 ttgtttactc ctcatccctg attgacaaat cacatagacg gtcttttctt tgtctccatc
    83761 atatctgttt atactttgta atggaacatt tttagctcct tttatatgcc cgccccggta
    83821 ttctgcaggt gttctaacat caagtaacgc tggattttcc aaaagctttt cctttaattc
    83881 cgggactgtt atcgatttta ttgattgaaa tagcaaattc attttctgca cctcatttac
    83941 atttttataa atacctcatg gggtatatta aacaggagct ttattaatat gtcaaccaga
    84001 atgactcttc atttttttca ctacttttta tatttgcata aaataccgat aaggggtatt
    84061 atctaattat aaaaatagag gagaatgtgt tatggagatt gataataaaa atatcctgaa
    84121 ccgtttgaaa cgtacagagg gacaaattcg tggtatccaa aaaatgatcg aagacgaaaa
    84181 agaatgtatg gatgtcatca ctcaattaag cgctgttcgt tctagtattg atcgcgtaat
    84241 gggcatgatc gttgcagata atctgaaaaa ttgctttgag aaacctgata gtaatccaga
    84301 agaacaagcg gctaaactag aacaagcgat caagatgatt attaaaaagt aaaggaatga
    84361 tttcaatgaa agtcgttttc catattgatg aattagaaaa atggtcagaa acagggaaaa
    84421 acgtaaaaaa cctgataaaa gcttctccag aaacgacgat tgtcgtatct gtaaatggca
    84481 tcgcgattac cggttatcta gactctgcca atgcagaatt cttagatttg caaggagtcg
    84541 tttttcacgc ttgcgccaat gctatgcgtg ctaatcatat ttcagaaagt tctttacctg
    84601 aacaagtaat cgttgtacca gcaggtgtcc ttgatttagt tgaactgcaa tcccaaggtt
    84661 atgcttatat caagccttag tcagtagagg ttgcgacaga agtgatcagc ttcaagaaat
    84721 ttcggcttat ttttcgaaga agctacttct ggaaaaccgt gaatcaaagg ttgtgaaaac
    84781 gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat agcttctcat atttttcgtt
    84841 tggttgggct tattaccgca gatgcaagct tttgaacacc gtttaaggaa gaggttgtga
    84901 aatcagcggt ttgacctaag tattagagaa aaaaacggga aaatagctcc tcatattttg
    84961 ccgttttttg agctaatacc gaaggtcagc tgattgaaca ccgtttaagg aagagattgt
    85021 gaaaaagtcg cttagtttcg agtattaaga aaaattttgt gaaaatggat ttctacattt
    85081 ttgcataaat ttggcttaat accgagaaac tggcttttga ataccgttta aggaagaggt
    85141 tgtaaaatca gcggtttgac ctgagtatta gagaaacaaa cggaaaaata gctgctcata
    85201 ttttgccgtt ttttgagcta ataccgaagg tcagctgatt gaacaccgtt taccaagagg
    85261 ttgtgagcct accatttaac tccgaatcac aagaaataat ttcaaaaaca acttctcctg
    85321 tttttggttt ggttggctta ttatcgagaa gtagctactg ggagtctatt tatcaaaaaa
    85381 aggaatgcga cataggtcac accccttttt tattttcttt gaaacatctt tcggtactcg
    85441 acagcataca agacaaaaga ttgtaaaaga ccgtaagtga tgacggcaaa aatgactctc
    85501 acccacagcg ttggtaattc tgggaaaagc agcaaaagga tcatgccaac atagatgaca
    85561 gcagtcgcta ctttgccata ccacttcgct ccacttaatt tgatattttt agatagcccg
    85621 attaatccag ctacaaacat aaagccttct tttatcaaga atagaccaaa caacagccaa
    85681 agccacggtc gataccatgc catagacaaa atcagaaaaa gttgtgtcaa tttatctgca
    85741 aacggatcaa gcaccttccc taagtctgtt acctgattat atctccgagc aatgaccccg
    85801 tctagataat cagacaaacc tgagacaaca atgaccgctc ctgctatata aaatgactgt
    85861 ctcagcacaa aataaagata aatcggcagc aaaaaaatac ggaatatcga taaaagattc
    85921 ggaatcgttt gtatttcttt acgccagtct gtgcttaatt tcattttatc gccctacctc
    85981 ctcagcactc ttttcttttt attttagctt acacggggaa taaaaccaat gatttcactg
    86041 tttttatttt tctttacagc cactgatatt atgagctaat ttgagataat cacttgctgt
    86101 tttcatcgtt tactcttctt tcttcctgtc gtttgcgcat ttcttttact agtactttat
    86161 gagctttttt tgatcctcgc tctaggtcac gctgccgttt acgtgtttta taatcgataa
    86221 acagactgtc taaatcgtaa cctgtcgggt acagatcttc tgctgaacgc accagacgaa
    86281 cacgctgacg aggcacgtgg attatttttt tgtctaaata tacatcaatc gtatcttcac
    86341 ctatatcttc ataaactaac gcttcttttt ggtattcatt gacaaatatc cgatcacctt
    86401 ttgcaaagag ctggcgttct tttttattct ccttcctatt tgtaactgct gtcttaggaa
    86461 gaaattcttt tttctgtgtc cgataggctt tttccgccaa gtaacgctct gcttgatgga
    86521 tcaatttcat cgacatatcc attttatgag caatccacaa agcttggctt tctcctgttt
    86581 ctccaacccg caactgatat ttaggacgaa gtgcttcccg atcgaaagcc atagcagccg
    86641 gcacaaaatc ttcatgatct ccggcgaatt tcttgatttc tccataatgg gtcgtggcaa
    86701 tgatcaatgc cccttgctca tacatagatt ccatgattgc gatcgctagt gcagcccctt
    86761 catttggctc tgttccactg ccaatctcat ctaaaagtac caacgtattc ctttttatct
    86821 ttttcaaaat agcagcgatg ttttgcatat gaccagaaaa tgtactcaac gcattttcta
    86881 aattttggtg gtcaccgaca tcgacgaaaa tctcatcaaa gacagcaagc tctgtccctt
    86941 cttttgccgg aacttgcata ccgaacatcg ccatcaaagt caataaaccg actgtcttca
    87001 acacgactgt tttgcctccg gcatttgctc cggtgataat caagcctcga tagtctttac
    87061 cgatttcaac atccaatggt accgcatgct caggtaaaaa tggatgtctg ccttgcttga
    87121 taatgatatg ttccgattta ttgattctag gtgtgcttcc attgatttct cggctaaatt
    87181 ttgcccgagc aaagatgata tctaacaccg tgatcgtttc gatcaataaa tcgatcactt
    87241 cttcgttttc tgcgatagca ccagtcaatg ccgccaggac ttgatattct tcactgattt
    87301 cttcagcttt taacaactga tagtgttcat tcaatttaga aatcacagtt ggttcaataa
    87361 aaaccgtcgt tcttttattt gattcatcga taatcgttcc atctatttta tttttatagc
    87421 tggctttgat aggaatcgta tagtattcgc ctttttgaac aatcattgct tcttggatca
    87481 tttctttgtt tttcggatga cgcaagaatt tcagtaattt tgactggatt tctttttcga
    87541 ttagttgtaa ttgtttgcgt actttacgca aattacgtga cgcgtcgtca ctgactttat
    87601 tattttttat tttctggtca atcaactctt ctacatttat caaatcagga agatgtttgc
    87661 tataagcaaa caacaagggt gtctggtatt gattcttatc aaaaaacttt gtgatcatcc
    87721 gactgcttct aagaaaatcc gcatattcga tcaaatcggt tggctgcaga atcaaccctt
    87781 ttttgacttg ttctgtcagt gcatcgatcc tcggcaaccc cataaatgga acatgctgac
    87841 tgctttctaa gatcaagcgt gcttctcttg tttcttcttg acgatccaag acagtttgta
    87901 ggtttgtcga gaccgtcata tcgctgattc gttttttgct atagtttcca attgctcttg
    87961 cttggatttc ctttttgatc cgatcaaatt gtaattgact aatcatttcg ttgtccatat
    88021 tctctccttt tcttgaagag atacctgtcc caaaataaat aagggcctag agataaataa
    88081 aaggatagaa cacagcttaa aaaagtcatg tcctatcctt attagaatac aaagatattg
    88141 ttttcctgac aggtaccgtt tcataagcac accaaataaa ctttgtttat tggctattcg
    88201 acttactgat tagataccag ctacactcac aaaacatacc ccattcttct aggtcttatt
    88261 tattttccac ttaactatag caagacacat tttggttgtc aaactttagt atgttttata
    88321 gatttacacc attaatgaat agacacctac cgcgataatt gctccgatca ttggacctgc
    88381 gattggtacc catgaatatg cccaatctga agttccttta ttagctattg gcaataccgc
    88441 atgtgctaat cgaggaccaa agtcccgagc agggttgatt gcatatcctg ttggccctcc
    88501 taatgaaaga ccgatggcta agatcaatcc acctacagcg aatacgttcg ttccaggtgc
    88561 aaattcgttt tgtccaaaag ctagcaaccc aagaactaat acaaatgtac caattaattc
    88621 tgtgatcaca tttgctggat aattccgtat agcaggacct gttgcaaacg ttcctaaaat
    88681 cgcgctttca tctttcgttg cattccaatg tggcaaataa gaaagccaaa caagaatggc
    88741 ccctgcaaaa gctcctaaaa cttgcgcaat gatgaaagga acgacaagat tccaactaat
    88801 tgcccctgtc atcgccatag ctagagagac tgcaggattc aaatgagctg ggcccatgaa
    88861 tccagaaata tagacagcca ttgttacagc gagtccccaa ccaaatgcga tgacaatcca
    88921 gccagaagcc tgtgctttac ttttgtttaa attcacagcc gcacacacac catctccaag
    88981 caaaactagg atcagtgtcc caataaactc tcccatgatt tgagtcatat cactgttcat
    89041 ttatttctcc ccctttagtt cccgcagatc tgattctgca atcaatgctt ctaaacgttt
    89101 agtctgttgc gccttctcct cttctgtcca ttcaaaatat ccagcaattg catcgatcac
    89161 aggttgcttg atctcatcaa gctgatctcg ttcaaacaac agatgatttg tacgacgaat
    89221 gagataatcg cctggggcca aaaccatttc ttcttctaaa ccataacgta accgagctga
    89281 ctcagcgaga ctcaaaccag gataaggtgc catttctttt gctaattcaa agatacgtcg
    89341 ggcattagtc ccgtaaaaat cagcgatata agtagcatct tcttcctcta aaccggctgc
    89401 tactccttct tttgctaatt ctgtcactgt ttcttctaat ttagttggat cgaagtttcc
    89461 acctgaaatc tgatattttt tagagtcgat ttctttcgtc tctattccgt attcttcttt
    89521 taataattgg cgtatcaatc gcaaagctcc ttctgccatc ttacggtaat cggtgatttt
    89581 cccaccagat aaagtcagca aaccatctgg ttctctttct aaagaactac ctctggaaat
    89641 cgtagaaggt gctttttcat cacggctgtt ttccaaatgg ttcaacacgt cttctacttc
    89701 agcacgagaa actttgtttt ccttatattc acttactgta tcgacaactt tattgaagct
    89761 cttgtctgaa atcgatccat tatctccacc attgtaatca gaaccagaat taccaatcaa
    89821 cagtggacga agtcctgccc agcttgcttc gatatcattc aatgtgatat ttgcttctgg
    89881 atagcgatga ttgatcacat ccaatagata atctacatct tcttgtgtga ctttcggatc
    89941 cgtaaaatct ccttggtaat ccgtatctgt cgtaccaaag taagtcttgt tttctcttgg
    90001 aatagcaaaa accatccgcc catcttgctt tcctgtatcg aagtatgtgg gttgcggtac
    90061 aggcagtttt ttcgcatcta caactaaatg gatcccttta gttggacgca ttttaggaga
    90121 gactggacgc gtaaaattca agtttcgtac tttatctacc caaggaccac tcgtattgat
    90181 cactaatttt gctttgattt cgatcacttc atctgtcagc aggtcacgag ctttgacgcc
    90241 aacgatttga tcgccatcat ataaaaagcc aaccgctttc attttactta ctagataagc
    90301 cccatcttcg gcagcctttt tgatattatc aatcactaaa cgggcatcat tgttgcggaa
    90361 atctagatag acacccgcgc cttttagccc ttcttttttc aaaaacggtt ctctttccaa
    90421 tacttcttct ggtgtcagcg tataattctc atatttggtt cctgtcacat ttgccagttt
    90481 gtcataaagg tccattgcta ctttgaccga gaacatattg aaggttgttg ctccttcgcc
    90541 ctcatagatt ggcaaaagca ttggatctgg ttttgggatg tgtggggcaa tactttgtac
    90601 gactgcacgt tcaccaactg tgtcagctac tacttccaca tcaaatgtct tcagataacg
    90661 aataccgcca tgaacaagtt tggtcgagcg agaggaagtc ccttctgcaa aatcctgcat
    90721 ttcgatcaat cctgttttga ttcctgatgc tgctgactgg actgccacac cggcaccagt
    90781 gattccgcca ccgatgatca acagatccaa ttcttcttgc tgcatttttt gaatgctatc
    90841 ttgtcttgtc ttgtttgaaa acatatggat ttcctcctta ttcaaatgct tgggtagctt
    90901 ttactgcttt tttccaaccg ttgtaaagtt tttctcggcg ctcttcctcc atttgaactt
    90961 caaatagctt gccagcttca taaaattcgc ggatctcgtc tgtatctttc caataaccga
    91021 cagctaatcc tgctaaaaat gctgccccta gcgccgttgt ttccaagttt tctgctcgtt
    91081 ggatcggtac attcaagata tctgtttgga attgcatcag atattcattg tttgctgcac
    91141 cgccatctac tttcaataca ggaatcttga ttccagtgtc atcttgcatc gtatcgatga
    91201 tatctctcac ttgataagca atcgactgta aagtagcttt aatgatatct tcttttgtcg
    91261 taccacgtgt caagccaaac atagcccctc gtgctgcctg atcccaataa ggagcaccta
    91321 gcccaacgaa tgcaggaaca acataaacct catcttcgct tgtagagcgt ttcgctgcgt
    91381 cttctgaatc gctagctttt tgcaacattt gaagtccatc tctcagccat tgaatcgaag
    91441 aaccagccac aaaaatactt ccttctaaag catagtaaac ctttccattt atgccgtaac
    91501 caattgtcgt caataaatta tttttggata attgcggttt ttcaccggta ttcatgacaa
    91561 taaatgaacc tgtaccataa gtattcttga ccatgcctgg ttcgaatgcc atttgtccga
    91621 ataatgcggc ttgctggtct cctgccatcc ctgaaattgg tgcttcgctt ccataaaaat
    91681 gataaccttg cgtataacca taaacttctg agttagaagt gacttttggt aacatcactc
    91741 gaggaatatt tagtaaatct aaaatctcct gatcccaatc tagctgatgg atattgaaca
    91801 acatcgtccg actggcattc gagtaatcag taacatgtgc ttgtccatcg gtcagtttcc
    91861 atactaacca agaatcgatc gttccaaaca tcaattcgcc tttttcagct cgttcttgcg
    91921 cgccttctac atgatcaaga atccagcgta tcttcgttgc tgagaaatag gcatcgatca
    91981 ctaaccctgt tttttcgtgg aaaaattctt gatagccttc attttttaag ttatctgcaa
    92041 ttccagctga ctgtctggat tgccaaacaa ctgcatgata gattggacgt cctgtatctt
    92101 tttcccatat tactgttgtt tcacgctggt tcgttatgcc aatcccagca atctgatttg
    92161 gtttgatccc tgattcgatg aacgctccgg caataactga ctgtacagaa ttccagattt
    92221 catttgcatc atgttccacc caactttctt gcgggaaata ttgcgtgaac tctttttgag
    92281 aactgccgat gtgtcttcct ttcttgtcaa agataattgc tcgtgagcta gttgtacctt
    92341 ggtcaatcgc catgatatac gttgattctg tcattgtgat tccctccatt atatgcaaac
    92401 gctttcttta actactctta tcatactact gttttaaaaa aagaaactgt caaaatttta
    92461 ttcttttttt ctttttttga cagtttttta ttatgaagct attttttaat caattctttt
    92521 cttttttcag cgtctctatt cttcttttca atcgaataat gtcgtaaggt gacgcaaatt
    92581 ctgacagata atattcacac tgttttttgt ttctctggca aaatgtgatc accaaatcat
    92641 attctttacc ttcttcataa cttccaatct caataccgcc gattcccctc agttctctga
    92701 tcagatattg ttgaaaagca atacgataaa tcggcagact ttctaaatcg attccaatca
    92761 ctactggttt tgcaatataa aaatcgatca tcaaaagaac attggcatac tggatcatca
    92821 ggtaactaag ctcactatct tcagctcttt tcctcggcaa ttgctgcacc gctgtttcct
    92881 gtaatttcgc cagtaactgt gttaaggaat gcccgagcat tttctgctgc tgttccaaaa
    92941 gccgatcacg ctcatagact tcgatctttc caacaaagaa aaaccattca ttgttgatct
    93001 gggaaatttg gtagcctact gccttttctt ctttgatttt caaacgacga ggacgataat
    93061 gccataacat cgtttcccga atgtaagtat ctaaaacagc ggttggtaac tttttgctcc
    93121 gtgtcaaatc atactgataa aatatttctt cgtctactat cgaaaaagaa acaaaaaagc
    93181 tgtaaaacat cattgtttcg tatacattca gttctgctgc tgttcgacta agatgcatca
    93241 cgatgattgg atcgattgct tgataaagcc ggtccgactg atagagtaat ttcttttctt
    93301 tcaaagttgc gtacgtactt ttttcattca atagtctttt tttcgtaatc ccgagccagc
    93361 aagcaatctg tgcttctgct gatgccgtaa acgttgtttc taatacacgg tccaatccta
    93421 gaatgaacgg tcttttttct ggtgtatttt gcaaaaaaag cggtcgctga tcttccggga
    93481 tatattggaa tagttcataa taaaaatatc gtatctgtag ctcttctccg tataattgac
    93541 cgtttttgat ttgtaatcca aattcctcta agagctggtt caattctttg atctttcgaa
    93601 aaacagaaga ctcactgata ttaaacgctg tcgctaatcg aacgatggat acttgctgat
    93661 gttctaataa atagacgagc atcttaaact tcaagctttc ctgcaataaa aacaatagaa
    93721 ttttttccaa acttacatct tcgtccaaca caagcagcgc ctgatactca tttcgctgta
    93781 cttctacttt tttttccatc attcggccaa tcaattcaat atcttccaaa tattgatcaa
    93841 acgaggcttt tgacagttgg acatgctctc tcaaaccatt gatagaggct gttccccctt
    93901 gcagaacaag gtttcgcaaa atcaggatct gtaactgctc ttttctttct aataatgctt
    93961 caatcttcat aggcagtatt cgccaccttt gaccacacca tatctggcac acggtattct
    94021 ggctggaaaa atagctcatg ttcttctaac tgttcaaacg tccatccttc ttctactgca
    94081 attgtcagtt ggtcaagaag gtggaacatc tcttttttgc tttggagctg tgctccaaga
    94141 atcttttttt cagaatcaaa tagaaatttg agtttaactt tgctttgatt tattgctgta
    94201 agcttgtagt ctacgtatcc tgtttcaatt ttttggggat aaaaaatgct ctcttcttct
    94261 gtcaaaccta cgcttcctaa gaaatagcca aaccactgat tgccgactgg acgataggtt
    94321 ccctgatcct ttgtctgtga gccactaatt gtcttgctga catttcgtgc tgtacggaca
    94381 gcgttattta ctagagaaac atacattttt tcatttgttg gacgaaaagt tacttggatc
    94441 gcatcgccaa tcgcataaac gtctttttga gacgtttgca agtagtcatc tacccagatc
    94501 gttccatcat cattcaatgt taattgttct tcccacatct gattgtcggg gcgagaatga
    94561 ttagccagca aaacaagatc atagttctcc tttctttttt ccgtctctac aatagcggtt
    94621 tcattttcgg tcaaagacac aacttgttct tctaaaaaaa gtgaaagccc ttgatttttc
    94681 atttccttta ctaacggttc tgtcatttca ggatctagat aacgaaaaag aattgacttt
    94741 cggctttcgt aaagatggat ttgttttttt cttgatgcca acccttcagc tatttcgatt
    94801 cctgcttgtc ctgcaccaat aattgcaatt ttctttgcat tcatgatctt ttgctgaaga
    94861 gccgttacgt tttgtatctt tttaactggg tgaataagcg ttgattctgc agaaatattt
    94921 cgaaactttt gatttgaacc agtcgctaga atcaaacgat caaaatccaa tttttttcct
    94981 cctgctaaaa cgactttttt ttctttcaat cctattactg tcttttctgt gtaaacatgg
    95041 attcgatagc ggtcagtcag ttcttctttc gtgatccaag aaagttgttt ttcactggta
    95101 acctttcctt ttaatatcaa attgaacccg ctgggaatcc atcctaactt cgattgcttt
    95161 tcaatcaaat atatttcact tgttggcatc aattggcgaa gataaatagc cgcatgaatc
    95221 cctgcaaacg acccgccgac aattacaatg ttcatctgca ttcctctttc ttataaggtg
    95281 ttctactgct aacttctcaa tcagaaaaca cagtattcat aactcgcttt caaccttatg
    95341 atacatagcg atgcagcaga atcaaccctc tccatcatac cgtgttctag gtctgcttct
    95401 caataatgta ctagtggacc taattgaaga gtatcatttc tgaagagatg tttcaaaaaa
    95461 tgtactctta taaaagaaaa aaactcgcct tttacgtacc tctgtccaaa taggaaacag
    95521 aaaagaacga aaagacgagt tttagcgtgt gtaatgtaat agttagaata gacacatcat
    95581 ttatttttaa ttctttaatg cttcaacaac tgcttcgcct aattgatgag cagattgagg
    95641 attttgccct gtaagaagcc gcccatcaat ttgaaccact tctgaaaaag ctgctccaga
    95701 ctgatagttt gctccttttt ctttcaaagc atcttctgct aagaatggta cttcattcgt
    95761 tgtaccattt gcttcctctt cttcattact gaacccagtc actgtacgtc cagctaaaat
    95821 agattttcca gactcatctg taactggcaa caatcctaca acaccgtgac aaacagctga
    95881 gaccagccct tcatttcgat agatttgtga agcgactttc ccaagatcta ttgcttctgg
    95941 aaaatcccac attgttccgt ggccaccagc ataataaacc gctgcataat catttgcatc
    96001 cacatcatca ggacgtaagg tatttcccaa ggcccgattt ctgaaatctt catcttcata
    96061 ataggcccaa tcgatttcat ccatattcaa aacactttgc ggttctaaag gtacatatcc
    96121 gcctttagga ctgacaaaat ctgcatcgat cccatttttt actaatacat cataaaaatg
    96181 agtcagttca cttaaccaca aacctgtagg acgctcgatc ttttcgtact tttctacatt
    96241 tgttaatacg atcaatactt tcattttccc atcctctttc tataaaaatt gcgttattga
    96301 taacgtgctt tcaagaaatt atataatggg cttggcaaat aaagctcttt atatgcttct
    96361 tcaaaaggtt tcgttacaaa ttcatcctca cggttttcca ataatttctc tgcccagtca
    96421 ggatcgacga tcatttgctg tccaattgct gcaagttctg catatttcaa tacctgctct
    96481 acatcttcgc gattgcggat cccaccgaca ccaactaaag ggatctttcc ttgaatcgtg
    96541 ttatggacca cttcaagaat cgatttatcg ttgtatttat cagaacgagc tattcgatca
    96601 tacatattta atgaaacatg cagataatct agttttgact cacgtaatcg ttcaagaagc
    96661 cagatcgtgt cttcaaaacg gatacctggt gtttcatatt cttctggtga gaaacgatac
    96721 cctacgatga atggtttttt agcatattgt tcaaccgctg agaaaaccgt tgttaataat
    96781 tcttcaataa aacgataacg attttccaag ctaccgcccc attgatcctc tcgtctattc
    96841 gaatgcggag agaaaaattg ttggatcaaa tacgtatttg ctccatgcaa ctcgattcca
    96901 tcaaatcctg attcaatcgc tctacgtgtg gctttgccaa atgcttggat tgtttcaacg
    96961 atttcttctt cactcattgc tcgtggtgtt tccgcatcag gacgttttgc tgcaactgca
    97021 cttgcagata caggctgttc tccagataaa gttgctcgac ttgtcattcg tccaccgtgg
    97081 aaaatctgaa taatcgcttt tgcgccttct ccttggattg ctttcgctag ctcacttaat
    97141 cgaggaatca tttcatcatg agcgatgctg agttcacctg gccagccttt tccgatatct
    97201 tggacatttg cagcaccagt aataacagca ccaagacctt tagaacgttg tttataataa
    97261 gtgatctcat cttcggtgat cactccattg aaaaagctga gttgtgtcgt cataggcgac
    97321 agcatcaatc ggttcttcaa ttgaactcct gaaggtaaag taattgattg atcaaattgt
    97381 gacattgtca ttcctcccat aattcgctct gattaaccta tcgagaatca tctatcatta
    97441 aaccactcga ttagttgtac atgcaactaa tttgcttaaa aaattttatt ttttttgtaa
    97501 cttatctgtt gcatcattca ttgtctgggc taattcttta cttgcttcat ttcccaaaac
    97561 tgctttgtat gactgatgaa gttcttccca gctaccatcg acttccttag caaattggcg
    97621 tccttgttct gtaagataaa ctaatgacaa gcgaccttcg ctaactgttt tgacgtatcc
    97681 tttatattct aatttttcaa caaaacgagc aatcgttgat ggggcggaat agagcatttg
    97741 tgcaatatct ttttgtggta ttccatcttt ttcttttaat atcaataaaa taaaaccata
    97801 agttggtgcc agacctgtcc ttctaaacgc ttcatcagct aatttattca acatgcgatc
    97861 tagctttttc acgctaaaga aaagacattc atctaacgct ttttcaattg gcttttccat
    97921 ttcatcccct ccattggcgt acatatcata gtatgatttt tattttttgt caaaaaacac
    97981 ggaataatgt ccgcctcctc tgtctagatt tgaaaaaaag aagtagccat cttcaaagaa
    98041 tttcggcttg ttttcgacaa aactccttct ggaacaccgt gaatcagagg ttgtgaaaaa
    98101 gtcgtttagt tttgagtatg aagaaaaatt ttgtgaaaat ggctttccac atttttgcat
    98161 aaatttggct taataccgaa aaactggctt ttgaacaccg tttatatcag aggttgtgaa
    98221 aaagccgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct catattttgt
    98281 cgttttttga gctaatgccg atggtcagct gattgtacac cgtttattga gaggtcgtga
    98341 gcctgccgtt gaactccgag tcacaaggag caatttcaaa aaacagcttc tcatgtttta
    98401 tgactatggt gacttgtgat cgaggagttg gcaggagaat actgtttatt cgtaaagaag
    98461 gggctgtgac aagacttttg tcacagtccc ttatttcctt ttattatagg atttcgccat
    98521 ttgattggat gacttgtctg taccaatcaa atgatttttt ctttaaccgt ttctttgtgc
    98581 cattcccttc gtcatcttga tcaacataga tatatccata acgttttgac atttgtgaag
    98641 tagaagcgct gacgatatca atgcagcccc acgacgtata accaaataaa tctactccat
    98701 ctttgatagc ttccttcatc tgctcaatat gctgtttcaa atagtcaatg cggtaatcgt
    98761 cttgaacggt tccatcttct tctactttgt ctatagctcc gagtccgttt tcagctatga
    98821 acagtggttt ttgataccta tcatataatt gattcaaagc aattctcaaa cccttaggat
    98881 caatctgcca gccccattca cttgatggaa gatacggatt tttaattcct ccagttatta
    98941 agtttccttg gaccccttcc caattttctg gatgagcact gactgcacta gacatgtagt
    99001 agctaaaaga aacaaaatca acagggtagg ctgctaagat ttcttcatca cctacttcta
    99061 tttttaagtt gacacctttt tcttcaaaca ttcgttgcgt atacgctgga taatatcctc
    99121 ttgcttgaac atcactaaag aacaaagaag ttctttgctg ctcaattgtt ttttgaatat
    99181 catccggatc acaagaatac ggaacaagaa gctgatagct gatcatacaa ccgacttgtg
    99241 aacctttgat aatttcatga caagcttttg tagctaaagc acttgctata aactgatggt
    99301 gtgcaccttg gtatttcgct tgttccaaat ggggatggtt ttcttcaatt actcctacgc
    99361 taacaaaagg gtgatgtttt acacagttga tctcattgaa agtcatccaa tattttactt
    99421 tatttttata gcgtttgaat actgtttcag cgaaacgaac gtaaaactga ataagctttc
    99481 gattaggcca tccaccatat tctgtaacca aatataacgg catttcataa tgggaaagag
    99541 aaacgactgg ctcaatccca tattttagac attcgtcaaa cacttgatca taaaaaaata
    99601 atcccgcttc attgggtgtt tcttcatcac catgaggaaa aattcttgtc catgcgatag
    99661 acatccgata gcatttaaaa cccatttcag caaataaacg gatgtcttct ttaaagtgat
    99721 gatagaaatc atttccgtgt cttttaggat aataatactt gctgttggga tcctgcgccg
    99781 cttttacttg ctgatgcgtc atcatatgcc attgatctgt ccatttttct ttaggtaaat
    99841 cgctgtcgaa tgtatacaca tcagacacag ataatccttt ttcatctaca tcccatgcac
    99901 cttcacattg attggccgct gtcgctccgc cccataaaaa tccttctgga aattttttca
    99961 tatcgatccc cctaaacttg ctagttttct tgtttgatta tttttcttat gactagtaat
   100021 gatatttttg aatctactac tatttgtaac aataaccatt gtggtcggat catatccctc
   100081 tttgattaat tcttgcaaat ctacttccat caataaatcg cctttacgta ctttttttcc
   100141 atcagatact gcactttgaa agtatttgcc atttaaattc actgtatcaa taccgatatg
   100201 gatcagtatc tctgctcctt cttcactgac taccccaatt gcatgtttcg ttggaaaaac
   100261 tgttgaaact tccccgttta caggactgta gatttttccg tccgttggaa tgatcgcaaa
   100321 cccttctcct aaagatccac tagcaaatgt tggatcgttt acttcagata aatcaattat
   100381 ttcacctgct gcaggagtac ttaacactat atcctttgag gattcttctt cgatttcatc
   100441 cttgtagccg aaaagaaaag ttagcataca ggaactgatc atcgctaaga aataactagc
   100501 aactagccaa acaaatcctt caccaatata aatcgggaaa gacaaaatac ttcttgtagc
   100561 aactgccaaa gcaccggctc ctgcggcacc agctactcca ccaaatatag ctccacatat
   100621 acaagcaatg taaaatggtc gtttatattt aagagtcaca ccataaacag caggttcagt
   100681 gattccaaat aatgctgtga taaatgcaga taaagataaa ctttttactt tcttattttt
   100741 cgttttcaaa aataccccaa gtactgctcc tgcttgcgca gctacggcag tcatacatgc
   100801 tggacctagc gtatcacgac cgtagaaaga aagattgttg gtcattactg gtacaagtcc
   100861 ccaatgtaac ccaaaaatca ctaacacttg ccaaaatccg ccgataacag caccagataa
   100921 aacagggcta aagttataaa cagctaaata tccagctgca atcagctgac ttgcataatc
   100981 tgcaattgga cctacaacta ataaagtaat tgggaccatc acgatgatac aaatgaatgg
   101041 tgtcaataaa tttcgaatac tttcgtgcaa gcttttgcga atcttaggct caatataaga
   101101 aagaacccat acagctaaaa ttgctggaat aaccgtactt gtataccttg ctagaatcac
   101161 gggcatccct agaaatcgca aggttatgct atcactatat gcactgacaa tcgtaggata
   101221 aacgagagct gctgcaataa ccatagcgat gaaacgatct gtattgaact tttttgcagc
   101281 agtatatgcc aaaaatatag gcaagaaata gaaaaatgca tcagctgctg cattcaaaat
   101341 gatataagtt cctgattcag tagttaataa attcaaactc gtacataagg cgagtactcc
   101401 tttgatcaaa ccacttcctg ccaagacgcc taaagtcggg gcaaaaacag cggcgatcat
   101461 gtctataaaa gagtttaaaa tgcctttttt ctcagttcct ctctttccac tagatgcatc
   101521 atcttctagt ttcatttgtt tggataatac gtcatacact tcatttactg catttcctac
   101581 tacgacttga aacattcctc cagcttcgac tacagtgatc acaccttcta gttgctctac
   101641 cttctcctta tctgccttct tattatcttt tagggtaaag cgtaaccttg ttgcacaatg
   101701 gtaaacattg ctgacatttt cttcgcctcc gacaaatcgg acaatatctt tcgctaattg
   101761 tgaatagtcc atttttactt cccccttgtt aattaatgcc gtagcaatcc ttcttacaag
   101821 ataagtatag ttcttagaaa acggtttctc cattcaatta gtacccctta taatagcgaa
   101881 cgaaactgta caatagtgtg tatggtttac ttagccgagc aaatcactca ctgcttgaaa
   101941 gaaaacaagt atatcgtttc tcttatagtc aaaggagaca aaaaaaacac gcttgatctt
   102001 cctttgctga tgtataagtg gtatctcaag ctgatactct gtgatttttg tttctacttg
   102061 gcttttgata agacagcaca acaaagtctc ggcatttaac ggaagcataa ctaggtcaga
   102121 actaggagag attagttgct gaatggaatc tatcttttta tggattttta tttgattttc
   102181 ttctttgtag taatgatcaa gcagttttgt tttctttttt tcttcaacta tggtcagctt
   102241 ttcacgcaaa atctgataaa tctcccaaga ttcaaactca tctagaacac taggtagata
   102301 aatcaaatca tcccactcaa aaataagctc ttgctcagta gtcaaaattg catcatattc
   102361 cgttattttg tttgattgat ttttgaaaag atacactggt tctatccggc attctttgat
   102421 ccacatgttc atactttgtt gaatctgttt ttttagtgta ttcaaaatac taattggctg
   102481 gttactaaca atcagtatct ccccttgttt tcgaaaagga ttcaacgctt cggcaagata
   102541 caaaacaaaa taagcgcttt cagctaaatt gggacgtaat tgaaaatgta ttgggaagaa
   102601 acgccgaatt aaataaattt ctaaaggata acgggagatg atcttttgta caaaatgatt
   102661 catggtatta tgtccggcat ccatcctcgt tttcatttgt ttgatatggg ttagcaaaaa
   102721 ttctgtttca tggaacaata acgaaacagg aagatctaaa aaacgaataa tcgtttgctc
   102781 aaatgctctt agattcttct ctatttcttt gtttttttca tcattaatca tcaaataatt
   102841 taactctaat agtcgatttt caattgcttg tttttcttga ttggaaaaag aatagggtag
   102901 ttctgatgat aacttttttg tgagggtctc taccatcgga ataaataatg tatacctgtt
   102961 tgttttttct aaaagttctt ctcttcgaag aatggtcaaa acgagatacc gagaaaatcg
   103021 tatgaagtcc tctcctgaaa caatataatt atatgatata agaacctctt ttaatgtctt
   103081 ttggacagtc ggcatgatat tggaaaaaat tgtactgatt tgttgaggca accttgcgtg
   103141 ttggatcacc gtttctgttc caacaagaga taagaaaatt ctttttgaat tttcttcagc
   103201 aatgattttt agtccttttg tcgacgaaac ttccagttct aatttaggat tgcgctcgat
   103261 ccaacgcaaa atcgttttta tttgtaatga tacggctgtt tttgaaagaa agaaaatgtc
   103321 tgctatcttt tgcatactga catacccttt ttgaaacaat aagtacgtca caatagttga
   103381 agctcggaaa tccatgctgg aatagatttt atgtttcgtc acctcttgta tgatttgctg
   103441 tttagtttca gattcaattg attctaaaaa ataacctgaa cgtttatttg actggataat
   103501 gattttcttc tcgtagatag caagcatatt tttgatttca gtttggatcg ttttcttgtt
   103561 ggtacctaaa gcttcagcta attcttcgct tgttatccaa tgattcttat ttcttaatac
   103621 tacaagaatt tggagttgtc gtttatttaa gtccatttgc ttacacctcc tctttgttat
   103681 aaacatacag gaaaaacctc tcaaatgaca atagtcactt gcagaggttt aaaaatcatt
   103741 tgaagaaaga tctatattca aggtttttac cattagactg gataatttgt ttataatatt
   103801 caaaactatc ttttttcaat ctcctaaagc ttccgctccc atcattttgt ctatccacat
   103861 aaataaagcc atatcgcttc ctcatttccc ctgttcctgc cgatacgata tcgattggtc
   103921 cccaccataa gtaaccaatg atttggcaac catcttttat tgcctcagca atttcctgaa
   103981 gatgtatttt tagatactca caacgttttg gatcgtggat tcgattcaat tcatctggtg
   104041 cttcatctag tccaatgcca ttttcaacta tgaataatgg taattcataa cggtctgtca
   104101 attcattcat gacataacga aaccctagag gatcaattgg ccaactccaa ggttcgggag
   104161 atttttcctt caaaaaggga ttatcgaaac catttgctcc gccagtatct acgcgcattt
   104221 ctgcttcttt tttatagaca gcgctacgat aataactaaa tgcaatataa tctgccgtgt
   104281 tagcttgcaa gcactgttcg tctccatcta acattactgg tgcacagtct tcttccttcc
   104341 atgtcctgta aatataagct ggataatgcc ctcggcacat gacatcggag aaaaaccatg
   104401 aattgcgttt aaattgcagt gaaccaaata cattctctgg atcacagtca tatgcataag
   104461 tcgctaatgc agaaaagcta cacataatac ccatcttagc atttggatcg attttcttta
   104521 ataattgagt agcacgggcg ttcgctagaa acatatgatg acaggcttga taaatcactt
   104581 tttttgttag tgtagcaatg ggttcaagtt tatctctagc agggaatgta accaatccag
   104641 cagctgcata aggcatacga aaagcattat tgatctcatt aaaggtcatc caatatttca
   104701 ctttcccttg gtagcgttca aagacggtct gcgcataaaa ttcaaaaaag tcgattaatt
   104761 ttcgattttg ccacccccca tactctccta ccaatgctaa gggggtttca taatgactaa
   104821 gagtaattac tggctcgata tcatatcgat ttaatgtatt gattagtctg tcataaaata
   104881 cgagaccagc ctcatttggc ttctgttcgt ctcctcttgg aaatattcgt gcccatgaga
   104941 tgcttgttcg aaaagctttt aaacctaact ccttcagtaa acgaatatcc tcttcaaatc
   105001 gatgataaaa atcaatcgct tcatgagaaa gataatggag tgattcgtct aaagcaatct
   105061 cccaaatctt tttttcttca ttccactgaa tactaggatg ctggttcatg atcccgataa
   105121 gcgtatctgt cacattaggt aatttgccgt cttccaacca tgcaccttca cattgattag
   105181 cagctactgc tccgccccat aaaaaatttt ctggaaatat ttcctgatcc ataactatct
   105241 ctcctcgcta tcaacaattt cttacttgaa gtatagctcg attcatcaaa acaagatatt
   105301 ctaactattt cggcagaaga gaagaaaaaa attgaatgct actcttcatt tttaatattt
   105361 atatataaca aatacataag acgatccccg aaagaaaaca ctcgttaacc atctaagcaa
   105421 acgatttatt ttacttccaa cacaaatgct tcataaggtc gtaaatcaac tttgccgtct
   105481 ggtaactgtt tcagaggata atttgacaca ataaccctta aagcttcacg tggaacagtg
   105541 aacgtctgct tttcactgga catattacaa ataacgagcc attgctggtc ttcatatatg
   105601 cgagaatagg caaagacatg ctcaggcgtc ttttccacta attcatagtc tccccagacg
   105661 atgatgtcat gttttctacg caaatcgatc aatttcttgt acatataaaa aacagaatct
   105721 tgatctgcta acgcttcttc tgcattgatt tctttataat ttggattgac tcgaagccat
   105781 ggaattccag tagtaaaccc agcatgttca cgggcgttcc attgcatagg cgtcctagca
   105841 ttgtctcttc ctttcgcatt gattgattcc aagatttcct cttttgtaaa tccagaagaa
   105901 atcctttcgt tatacatatt gattgtctca atatcttttg cttccgcgat atcatcgatt
   105961 gggcaattcg tcatgcctaa ctcttctcct tgatagatat aaggggtgcc tttcatcata
   106021 tggagcaaga tagcaaacat cttggcactt tcttttcgat acacatcgtc atttccccaa
   106081 cgagaaacaa ttcgcggaag atcgtgatta ttccaaaata aactattcca tccttcatca
   106141 cctaaagatg tttgccattt ggataatact tttttcagtt cagagatact caagggtttc
   106201 aaatcccatt tgtctttgcc ttcttgctga tctaatccta tgtgttcgaa ttggaagacc
   106261 atggataatt catttctttt aggattggaa taaagtttag cgatttcggt agttgctccc
   106321 catgtttcgc caacagtcat cagatttttg tcaccaaaag ttgcttgatt catttcctga
   106381 agatactcat gcagtttagg tccattactt gtgatttctt tatccggttc tttgccaatc
   106441 atatcgatca cgtccatacg aaagccccca atacctttat cgatccagaa attcatcatt
   106501 tgataaatct cttggcgaac ctgtttattt ttccaattca aatcaggttg acgcttgcta
   106561 tacaagtgca gaaaatattg ccccgttttt tcatccaact gccaagcaga accactgaat
   106621 attgagcgca atctattagg ctcttttcca ttgaccggat cacgccaaat ataatagtcc
   106681 cgataaggat tttctttatt tttacgggcc tcaatgaacc acggatgttc atcagaagta
   106741 tgattcacca ccagatccat gatgatacgg atatttcttt tctttgcttc ttcaatcagt
   106801 tcttccattt cttccatcgt accgaattct gccatgattg cttgataatt actgatatca
   106861 tacccattgt catcattcgg agactcgtat actggagaaa gccaaattgc atcaatacct
   106921 aaatacgcca gataatccaa cctggaaata atcccccgca aatctccaat accatcttga
   106981 tttgaatctt gaaaacttct tggatagatt tgataaacaa ctgaatggtg ccaccatttt
   107041 tgttccatga ttttcctcct ataaatggat gtttctattt tttgcttaat catttatttt
   107101 agtctctctc actaaaaatt gtaacgcttt caaatagcta ttgcaccttg ttatcggtaa
   107161 cagaataaaa caaaaaaaca gctgaggaaa ttccctcaac tgttttttga aaggattgat
   107221 ttatgcaggg aagaatgctg atttcacaac ttctttataa acggtgttca ctcagctgtc
   107281 ccttggtatt agctcaaaaa acggcaaaat atgaggaact attttttcgt ttttttctct
   107341 aatactcagg ccagaacact gatttcacaa cctctttata aacggtgttc actcagctgt
   107401 ccctcggtat tagctcaaaa aacggcaaaa tataagaaac tattttttcg tttttttctc
   107461 taatactcag gccagaacgc tgatttcaca acctctttat ttcacattca atatccacat
   107521 gaatcccaca aatacaaaga acagaccata catgattgga gatacttctt ttcctctttt
   107581 agctgctaac atggtaattg gatagaagat aaaacctaat gcaatcccat ctgaaatact
   107641 ataagtcaaa ggcatgccga tcaagatcaa gaatgaaggg atcgcaatct ctaagtttcc
   107701 ccattcgatt tgtcgcaaag attgtgccat caatactcct accataatca atgctggtgc
   107761 agttacttgt gaagtaacaa ctgatagtaa tggagaaaag aacaagccta acaagaagaa
   107821 aatccctgta gtaatagctg tgatacctga acgtcctcca acggcgatac cagctgatga
   107881 ttctacaaat gcaccaacag gagaagtacc aaataaggaa cccgcaagca tagctgttga
   107941 atcagaagct aaagctttcc ctacacgagg catcttgttg tctttcatga aacctgcttg
   108001 gtttgctaat ccaactaaag ttcctgctgt atcaaagaaa gtcacaagca agaatgtcag
   108061 cactacgacc cacatttgaa gagaattgat gtcccctaca tgttttaatg caactaaaaa
   108121 tgttggtttc aaacttggtg ctgcggcaac aatttcagaa ggcattggaa tcaaacctgt
   108181 gaccagacct aaaatcgttg tagctgccat cccgatgaag atgccacctg gtacacgccg
   108241 aacaagcaag atagcagtta cgactaatcc aaaaatcgtc aaccaagtag aaccaacgtt
   108301 gagtgatcct aatcctacta atgtagattc attcgaaacg atgatcccac cttcgctcag
   108361 accaaggaaa gcgataaata gaccgatacc gccagaaatc gcatatttca aatctgctgg
   108421 gatcgcatca atgatcattt cccgcagctt gaagattgtg atcaaaataa aaatcaaaga
   108481 agcaacgaat acgccggcta atgctgtttc ccatggaatc cccattccta aacaaacaga
   108541 ataagcaaaa aaagcattga tcccaagagc aggtgccgtt ccaattggat aacgggctaa
   108601 tacccccatt aaaacacagc cgatcgcact ggctaatgca gtcgcagtaa acactgctcc
   108661 ttcatccatt ccagaagcac ctaatacagt tggatttaca aataagatat aagccattga
   108721 gataaatgtt gtaaatcctg ccagtacctc acgttttacc gtggtgttca atttttctat
   108781 ttcgaagtag gaaagtattt tttccttcat cttccatccc cctaaatccg aacattataa
   108841 ataaagtccg ttaaaatttc gatttattaa taaaacaacg tgttttatca tatactaaaa
   108901 caaaacataa gtaaacaaaa aattcggatt ttaagcaaaa tccgaacgtt ccatctgatt
   108961 aaatcccttt atctcaaagt tcatgaacat tcactttgga ttacaagaat ttctttattg
   109021 tgttaataaa gtttcttcat tctagtctca aaaatcaata gtgtgatttg attttaccat
   109081 ttcgtttctg ttcttctttg atgaattctt ttgtatcgac aggattcaga ttatttagtc
   109141 cgccaaaacc tgtatcgacc atttttcccc agccttttgt cttctttttt cctgatttga
   109201 gattctttgc tcgaagcttc tcactatgtt ttgctcgccg atacttcaaa taaccttcta
   109261 atcttcccat tagatccact gtttttcctc ctttttattc aatcagacga ctcagctttt
   109321 cctataacaa aataattgcc ttcagggtca gaaaaattga aagtcaagat gccattgtta
   109381 tctgtgattt ctcctggttc ttccatgttc tcgtagatag tttcaaattc atccgtaaaa
   109441 aatactaaag aaggcggtgg tcctaatacc tctggtgaaa acttctgcac aaatactttt
   109501 gggaatacgc ctaattctaa ttcttttgag atgctgagta caatactctt aaattcttct
   109561 ggcaattcga ttgtttcaga aatttctgca ccaatttttt ctacaaagaa tcggcaaatc
   109621 atctctacgt cattgacata aagcatcaca cgtgtttttt gtatcatcat ttgacccttc
   109681 tttctatttg attttctttc ctgtctataa actgattcca ttatatcacg aaagcgcaaa
   109741 caccttgtgt ttgatactat tcaagaaaaa taaagcaata gtttctagga ttgctttcct
   109801 ccacaaactt tttttacaaa aaaaaaaacg gcaatgaatg ccgctcttct tatttcgatt
   109861 cttttataat tctgcttctg attcatcttt ggttagtgtg tgtctaacac tagttacgcc
   109921 aaatgtttga tcgatttttt ccttcaacgc ttcgattgcc tgtgcgccac gtgccgcttg
   109981 ttcagcattg atggattcaa tgaccagcac cgctgcttgt gtttcttccg tcagcattgt
   110041 tctttgggct tcacgccagt tgagacagcg acagatcgct ccttgttcat catagtagat
   110101 gatttcttcc ggaagtgccg gagcatcgga atctgcacct aaaggcagga atgattcgcc
   110161 accttgggct tttcctaaat gcagatttcc tgcgatcttt tcgatatctt ctccgccgca
   110221 aggtacgcca aaagacaagg aaatactatt atagatatct acaagcggat tgattgggaa
   110281 aaattcccgt tcttgatttg cacgtttcag taaagcttca atagaagaac gtgcaccttt
   110341 tttagtcttg aatgtggtaa atgcctgacg ccactgagca atgacttcgt tttggctgaa
   110401 tgtttcttct gtaaggtaat ttcttgcttc ttttgctgcc tcattcaata attgatgata
   110461 cgtttcttca ctttcttttg ttacgtggtt atcgatacca tttacggata agatttcgat
   110521 ggttgcttca ggaaatactt cccagaaatt ggtatccact attacttttt tcatctgttc
   110581 acacctcttg attagaataa ttgcttgatg aatagatagt ctgtttgggg atcatctccc
   110641 atataaaaaa catgttcgct gtatttgata aatccttttt tacgataaaa agcgagcgca
   110701 acatcgttgt gttcccaaac accgagccaa atggctgttt tttgcttttc ttttgctttt
   110761 tcaatagctg tatctagtaa aaatgtaccg ataccttgat gtttaaattt ttttcgtaca
   110821 tagattcgct cgatttctaa aaaatgatca gccatttcct cagattgact cgttcctgta
   110881 tttagtttca ggtaacccgc aggctcttga tcgatactca ctaggaaaaa atcggtttct
   110941 ttatctttta gctcttctgt aagtttttct agtgcataag cttgtgtcaa gtaatcagct
   111001 aaatgctccg gtgaattatc ttttccaaat gtatctgtaa atgtttcgat actaatttgt
   111061 tgtagtaagt ctacatctga aggcgtcatt tttcgaattt cgtatttatt cacggcttct
   111121 tcctttcaat agatcctttt attgcctttt ttgacaaaat tccaatcttc gctaacattc
   111181 ttgcatactt tatttaaaag atattccaat tgttttgctt cttcgtcaga aagaccattt
   111241 aatgcgactg cattggagta gttgttttct cgaatgatga aaggatagat ttctgctcct
   111301 tttttagtcg gatagagttt cttgattttt ttattttctt tatcttccaa acgttcgatc
   111361 atcccattgg attctaattt cttgattgct cgggcagtcg ttgtacgatc tactttgatc
   111421 atttctgcta gtttttcttg gatgattcca ggattttcgc aaatacgtac taaataaaga
   111481 tactgtcctc ttgttaaatc gacttctttg aattcgatat tgctaataga atctagcgct
   111541 cgggcaatca ttccgatgtc tcgaaggatt tcagccattt catcccacct ttattttgtt
   111601 tgtaagacta gtttacctct ttttgttgca aatgcaacta ttttcaataa aaaaatcaag
   111661 ttctttttta ataactagta aagtatattc ttttcttctt ttacgaaaaa gctcaatcac
   111721 aatagatctg gtttttgttt tctaaaataa gtaactgttt cttcgttgtt cgcttttctg
   111781 atagctttgg caactcttct atactaaaaa atcccatgtt ggaagtttca ttattctcaa
   111841 taaacttggc ctcttcaccg tggatcgcac aagcaaaaac aagtttataa tactgaaaaa
   111901 gctgtggaat atctttttgt ttattcgtat caaaaactgc tcgcaactga gtagtttcta
   111961 ctgttaatcc tgtttcttca tagacttctt tacgcacatt ttcttcagga gacaatccta
   112021 tttcagcaaa tccaccagga agtgaccatt ctttcgtccg tttatcttcg attagtagga
   112081 ccttgccatc ttttttgata taggcacgaa catctacttt tggtgttgga tgtccttctc
   112141 caatcgttgt gagcgctttt atttcttcga aagtttcttt tcctatttca ctgatcagtt
   112201 ccaatgaaat cgtccggatc tcctcaaaac gctctttatc aaatacatct tttccataat
   112261 atagaccagc ttgagaaagt gcgagtagtc ttttgtattg agccaacttc ttgttcattc
   112321 tcttcccttc ttttttagca aatgtatgac tatgtaatca agtatagaag actggtttaa
   112381 ttacggcaaa taaatataat gaaatctgca ataaataaaa gaatgttaaa aaactcgaga
   112441 acaaaagaca ttcgacttct ctggggctgt gaataaatgg cgttcaaaag cttgcatctg
   112501 cggtaataag ccccaccaaa cgaaaaatat gacgagctat tcttgtctgg ctgttcttat
   112561 taattgaggc aggacagctt tttcacaacc tccccactaa acggtgttct cctaccaact
   112621 cctcggtcac aagtcacaat attcatcaaa catgagaagc tgtttttttg aaattgctcc
   112681 ttgtgactcg gagctgaacg acaggctcac gacctctcgg taaacagcgt tcaaaagcca
   112741 atttttcgat attaagccat ttctttgtta cttattgtca ttgatttctt tttctaattt
   112801 atccaatagt ggagtgtaat ccacttgagt atagtctgtt acatcaaatt catatagtgt
   112861 tcctaactgg aatttgtcat attttcggtc ggcgatgatc tttttgaatg cttctttcgt
   112921 aatatgattg gtccctttcg ttcgatcttc caatacgtca ccatagtgat aatgatccat
   112981 gatatagctc aaatgacgat ctgattcacg atcacgtttg taccgacgtt gacataacac
   113041 ttcaaagtct gctgttaggc gaatcgtgat cacctgatat ccgtatctat ctgctaattc
   113101 ttggagtcgt gctttttgct tatcactaaa aggatactcg gacacaatca ctttctttcc
   113161 tgcttccata tacaaatcaa gtgctttgta atagaaagga taaactttcg tcctttcttg
   113221 ttccgcccgt tcttccaaat tgttaaaccc gatggattct gcatagtatt ccttgatttc
   113281 atcgagcgtg atggcataca tatctggaaa tcgttcccgt agtttattca taagatatgt
   113341 ttttcctgta ccaggactac cagccagaag aatgaaatac tttttcatct catttatttc
   113401 ctaagttaat tggactttcc aagttcataa acagtccaat cttatgcttg acaatttctt
   113461 ttactgcttc atttgctgga atcacattat ggcgaagaga cttgttgatt tctgtttcac
   113521 taaaggtttc tttagctact ttcgtccagt tgactaaaag ctccgttcca acattgaatt
   113581 tgcggatccc gttattgatc aattcaggat aatcttcttc tttcacacct gttcctccat
   113641 ggataactag ggggacatca actactgcat cgatttcttt cagcaaggga atgtttactt
   113701 ctgtttttga tttgaattgt ccatgattcg taccgatagc aatagctaat gcatccacgc
   113761 ctgtagcttc tacaaatttc actgcgtctt ccggtcttgt atataccttg tcattttcag
   113821 cgacatggat tccttcttct gtaccgccga ttgttccaag ttctccttct acggaaacac
   113881 cgtgcgcatg ggcatattct actactgctt ttgtcttcag gatattttct ttgaaaggca
   113941 gatgggaccc atcatacatg acagaagtat atccgctgtc gattgcttct ttgatatcat
   114001 caaagtctcg ggcgtggtct aagtgcaaga ctgcatcaac catttcttct tctgcaatag
   114061 ctgtcattac attgactaac acatgcatgc cgatatattt agcggtatcg acacttgttt
   114121 gaataatgat cggcgaaccc atttcttttg ctgcgcctag catagcagga agcatttcta
   114181 ggttatgcgt attaaaagct ccaactgtaa aattcaactc ttctgctgtt tttgttactt
   114241 ctttcaatgt tgtatacatc ttttattgcc accttatctt tttatttgtt tcctttgatc
   114301 gttacttgtt ttgcaatttg tcgcatctca tccattttgc ccatgtagta atcgatccct
   114361 tgatcatcac cagcacgagc agcatctgca cgcttctggt tatagaggct catcagtttt
   114421 ttatacccat ctccgatcgt ctgtttattt tccaaactct tttctaaata ttggatggct
   114481 tgatcgttca catacaattc agaaaattta agtccaactt gttcataaag tttcgctaat
   114541 ttatgcttat cttgggtagt ggaaatctct tgtttcaatt gtgtaatctc tgattgaatc
   114601 ttttcggtcg cttctggcga tagggctttt tcttcttttg ccagaacttt tttcggttct
   114661 ttttcttttt tcttaaaaaa tccaaacatc attttcccta ctttcaatta aaatgctttc
   114721 aaaactgggc ttaagatcca ggcatataag agtgctgcgg ctactgtatc tacatcggtt
   114781 gctgtagcat tgatgaatcc catcgaatta aagatcgtta ctagcaaagc gggcaataat
   114841 gtaataaaga aaccatgagc aataccgcca atgatcgctc ctcggcgtcc gccaactgcg
   114901 ttgccaaata tccctgctgt ccctccggca aagaaattgg ttagcatacc aggtaaaatc
   114961 atcgctaagc cgaacatggg caaagtaaac atggcaataa tcgtaccaat cgttgtcgta
   115021 atgaaaccta aaatgacagc atttgggcta taaggaaaga aaactggaca atctaaggca
   115081 gggatcgcat caggaactaa tttcatagct atccctcgaa atgccggcac gatttcacct
   115141 aacagtagac gtacaccagc tagcaataca tacacaccca ctacgaattg gatggcttgt
   115201 aaaaaggcaa acatgatata gttttgatct cctgataatt cagaagcgaa tggttcgcct
   115261 gcaaataaaa cagtgatgat atatagcggg accatcacga ccatgacaga caaataagta
   115321 tcttgcagaa attcgaaatg tgtcggcaac ttgatatctt cgatcgactt tttattttcg
   115381 cctttctcac caaaaagctt agcaacaccg gcttcaaata agtacccgat cgtacaaaaa
   115441 tgacctaaag caatatcatt tgatccagtg attttacgaa tgatcggttg cgctactgcc
   115501 ggcatagcaa tggcgaaaca agctccgata aaaccaccga ccaagatcaa tacgatccct
   115561 cgcagaccgg caaaatagcc aaagactgtc gtcatcgttg ccatccataa aattgcttga
   115621 ccagttaaaa aaatgtactt ccattttgtg aaacgagcca aaataatatt gaatacaaaa
   115681 atggctaaga acgtcagtgc aatatctcta cctaaaccca aatcattcat tgcttgacca
   115741 ttgatagatt caatagaagg gatgatccct tgcatatgaa atccttcagt aaagattttc
   115801 ccgaaataag ttaaacttcc aacgatgatg ctcgaaccgg cactcaacac ttgaaatcct
   115861 aggagtgtct ttaacgtgcc agagatgact tgtcctgctg atttcttctg cagtagtaga
   115921 cccaacatag caatcaatgc aatggtgatc gatgcctgag tcaaaatatt ttcgatgata
   115981 aaattgataa cgcccatgtt tttaccctct tttctttttg tatttagata actcccttat
   116041 cttgcagaac tggggtgatt ttttcttcaa tttcattttt tgaaacaata tttttcaaat
   116101 agatcatcgc cgttttagga tcaactgcga acttttcgaa ctgcgaacga aaattttctg
   116161 ctgtgatgat cacatctggt ttcatggaaa ctgcggaaga aatatcacaa tggtccagtt
   116221 tggcttctac tcctaatttg ttcaaaacat cctcagcaga catctgtgct gcaaaactac
   116281 ttcctaaacc tgcaccgcat acgaataaaa tgttcaattt gttcatcttt gttctccccc
   116341 gtatcattta ttatttctca actactgaaa aaagcagttg tttgaaatct tcgatatttg
   116401 tgcattcaat caatcgatcc aacttctctt cgttgttgat caattcaatt agttctttca
   116461 tgatgttcag atgcgagtag gagtctactg ctgccaaaca aaaaataatc tttactggat
   116521 ccatttcagg attaccgaaa tcgattggtt gctcaatcgt agctacgctg actcctaatt
   116581 tattgacccc atcttctggt cgggcatggg ctagtgccat gtgtttgcca atcacgatat
   116641 acggtccgta atcattcact gcacgaatca tcgcttcgac atagcgctct tcgacaatgt
   116701 tttctttaac caatggtcta gcagctagtt caatcgattc ctcccaagta gtggcttttt
   116761 ctttgatcat gatattgctg tccgtcaaaa tatcttttaa cataggctgg atctcccttt
   116821 tgttgatttc taaattgttt gtttcgaata atttttctaa cttgccatag atgattcgat
   116881 ctactcggcc accactttct ttcatgacat ctattatttg agtgaataac tccgtcgatt
   116941 catgactgtt aggctctaac cgctgtagtt cttcatttct tttcaaaaag tcttctataa
   117001 ccaagatatt cgtctcagtt aaaatcggat cgacaactaa aagcggctta tttcgataat
   117061 tcaattgaaa ggtgctgaaa accagattga catccaattt atctactaat tcgatttctt
   117121 ttgaacttaa aactgcagta atctcgatct gaggaaactt ttctttcaga ttctctgcta
   117181 acagatttcc agtagccatc ccatggttac agacaacgac tgatttataa acataattca
   117241 agtctcgctt gattacactg gcgatcgtcg aaaagtggat gacaagaaaa gctatctcgt
   117301 cctccagtat cttgccacca attacttctt cgatcgtagg aacaaaatga gtgactgcgc
   117361 ggtagattgg gaaataattt gttcggatgt tttctttcaa tggattcatg acttggatat
   117421 gattcttgat tcttacaatc agtgcagcca tgtgcttata gagattttcg aataaagctt
   117481 cttcctttag atggaaaggt acgtgtgtct cgttctctac aaattggatc aattggatcg
   117541 ttagtaattg agcttgtacc cactcgattg aattggagat atctctagca ttgaacgtct
   117601 caatagtgaa acgaagataa tttctttcta cttgcgggac attgtccaat tgaaattcct
   117661 cgatcatgtg gtcaataaat gcagaaaagc tactgtcttt tatttctgta tttttccaat
   117721 taatggcaga gataaattca tgtttcctga ttcgctggat ccagatcaat gtaaaaagca
   117781 ggatttgttt tttataaata tcatcttctt gtttcaacaa cttattggaa tagatctcct
   117841 gtattttttt gatttccacc aaaggaaaat aatggagaaa gattttttga gccggtgtta
   117901 attgtgcatc ttgattcata aagaagtcgg tcattcccag attctgattg ataaaatcat
   117961 agatcatcgt acggatagat cgttctgaac ctttataaac cagtccttgc tttccataac
   118021 tgacgatctc tatcccataa ctgaccagtg ttgctcgtaa ccgtctcata tcttcatcta
   118081 atgtactttt agagatttga aattcttctt cttttttgtt tagatggatc gggattcggc
   118141 tgtaagcaac ggctaaaact agatcaaaaa tacgttcgcg ccgattcaaa aagccgtctt
   118201 tcacatcttt tatttcagtt tctaatcgag cttgcttttc cttttccagc ttcaacaaaa
   118261 aaactttgtt gcgaattgta cggatctccg gaagcttttt actgctcaac cagatattcg
   118321 cttcaagaac ttcctgacgg atcgtccgct gactgacatt gaactcactt gctagatgtt
   118381 ttgcttttac tggatattgg gctgtcatga ttctgtgaag tatttcaatt gttcttgttt
   118441 tcatagaaat gatctccttc tgatttcaag tatatggttc cgcttacata ataaaaagta
   118501 gatatttctg ccacttgaag tggaaagaaa gtaagcaaag caaacaaaaa ataagccata
   118561 tgctttttca tctcctcaac gaaaaagaca tacgcctatt attacaaaaa ttttgcccat
   118621 ctatacaaaa aagaggttgt gaaaaagctg tcctgcatca agtaataaga acaactaaac
   118681 taaaatagtg cttcctattt ttcgtttggt tgttcttatt atcgcagatg caagctttta
   118741 aacaccattt agtggagagg ttgtgacaga gatgttcagc cttgagcgat aagaagcgat
   118801 tttgaaaaat cgcttcccat attttacaaa atcgtgcctt attgccgata ggctatctct
   118861 ggcacactgt ttattcagaa aaaggagctg cgacaaggct tttgtcacaa ctccgttcat
   118921 aaatatttcc tgtcaatcca gcataatcgg ctgaataaaa tcggcttgcc atcttttttc
   118981 tttctcagaa aaatcaaaat accatatctg agaaaaatac tgtcgttgtg tcttacttcc
   119041 tgagatcaat cgcccatctt ttcgatcgac tgaataatca cagcatctga aatcaattcg
   119101 gatggcaaaa ctattttcgt ggatctgtcg ataatttcct acttcctgaa tagttatttt
   119161 tttagtatga tttcttacac cgtctgcttt gtttttttgc agaactttct gatgttcggc
   119221 aaacagcttg tctgtataac agcttctggc tttttcagga gtttcttctt cccaagcgga
   119281 ttggatcttc atgaatatct tttggatttc cagaaactgt gctttctttt ctttagccgt
   119341 tcctgacatg cgggacaata tctccgcttt cagccttttc ttttgtcttt tattcttgac
   119401 gatagaaaaa gcagaaaccc ctcctactga caaaacagct aatgcaagaa atcccgtttg
   119461 ctcataacta gaaggacctg tacctggacc ataataccca taagaatctc tgccgtaaga
   119521 acgactgcca aaataactgc ccccatatga acgaccacca aatgaacctc tgcccgaact
   119581 tcgaccgcta gatgtacctc caccatgacc acccgcgaca gcgaatcctt gggtcggcag
   119641 agcaaacaaa cataggccca ctaacaaaat gattgctaaa taattgattt ttttcattgt
   119701 cttcctcgtt atcttttatt acaaaaaact atttttatga tagccttgct aaaaggaaga
   119761 gtcaataacc agatgcataa actgtttttt tagcaatttt gtcttattta gtcattgagt
   119821 aacatcggac aaatgtatgt gtcggcagtc cataattcct ctatcttcag catgaacaag
   119881 cttgcagaca agagatctgc gcttcctcca ggagagattt ggttttcctg acaaaagtgc
   119941 aacagtgctg taaatggacg atttggtaaa gcactttgac tagcagcatg agcaagtttt
   120001 tgcatttcca ctaacgtttt ctgatcactg cgatgcaata cgcatgtgtc atctagcgaa
   120061 ctcatcaaaa ctaacaacat atgcatttgt ttctgtacat atgaatcatt atctagctcc
   120121 ttagctgctt ggattgcaag cctgatatgc ggatacccta atatcgcttc ttcatatgca
   120181 ccatttacgc catatttttg cttcacccta tgaccatgag tggttttttt atgacgatat
   120241 tttgtatcag gaaaagcagc aagcttgctg acatcatcaa gtattttcga taaagaaaaa
   120301 ggctgttcca tactgatttt ttgtgcaatg acactgatca atagccccat tgcccaaatc
   120361 gctcctttat gggtattgat gccatttgta gcaatcagca ttgccttttc cgcttttcgc
   120421 ccaatctcag caattctttc tcttaactcc tgatcaatag catgatgcca tgctatttga
   120481 gccatttctt caaaaaaagg agttagcgtc ttagcagact taagaaataa agcatagtcc
   120541 atatcggaat gtgcacccgc atccagtgca tccactaatc caggttttgg acttaatgtt
   120601 acttctgcga tcaatgcttc ctctaccaac gcagcgagtt gctgagtcac tgcttttttt
   120661 cgttcgtcat ttatcatgac actcactcct tcttcctact attacttgct gaaacctttt
   120721 ttgatagcta cccaaatgat atgaagtaac cagcttaaag caaatgcaca acttgccgtc
   120781 attagataag ctacaagaat catgactcct gaaggtaact gcagccaact atctttaaac
   120841 aataataaca gtacttggaa aacaaataca ttggctaaat acgtacgata agcataaaca
   120901 gacaaccatt tgaacaatgg caattttggc gaatcgttta caatcatttt ggaagcgcct
   120961 atgaagacag caaagatgat gactaaactg tataaacttt ggatcgtatt caagtaaggt
   121021 gcattcgcaa agctcaagtc tcctggatgt gcgagcaatg aacggacaga aaagaacgcg
   121081 ataactaagg caacaggtaa gaaaagataa cgtactctgt ccaaatattg atagactttt
   121141 tcgtgataaa caaatgcggc taccccataa atcatgaaac tagcaacaaa ccgatctaat
   121201 aagtaccagc tgtcatgata aggcccagtc aatacttgtg tttgatagaa cagataccat
   121261 gcgacatata aaatcgttga accaatcaat actggccata catatttttt attagcaaat
   121321 actttgtaac cgagccaaac aaaaaacggc atgaacaatt ggatctgcag catcataacg
   121381 gtataccata aatgaggcgc tccatcacct gtgacatatt tcagtaaaaa gcttgcagta
   121441 tccgtatacg gagtttgttg ttggactgct ggaaatagcc aaagataagc cgtcgtccat
   121501 agaagatacg gaacgactag ttcaaaaaac ttttctttca aaaattccca ataagacaaa
   121561 tgctgacttg tttttaccat attataaaca attgcaaaaa taaacattgg tgctgtgtat
   121621 ttaacgatga cgtaaaaggt accaagaaaa gctgtatttg ttgcgctgtg gctgtttgcc
   121681 aaagcaaagc tcaatatcgt ttgcatcata acagccgtta cagctgatac tttctggaag
   121741 tcacttacgt tgattttgat ttttttgttt tcaaacatct ctttctctct ctttttcttt
   121801 attaccaaag ttctttggtt aatttaggtc cttcgactgt tttcaataga atttttttct
   121861 ctggctctct caaatactct tttagtgaaa aaccgccttg ccgagtcact acttgggtat
   121921 cgaattgctc aaaaaaagca ggatgcgatt gaattacctg aattggcaat tcaatcggtg
   121981 aatttgcgta cagcagcaaa tcaaaatcac tggtttcttt tactgccgga atcccagtcg
   122041 ataattcaaa tcctaagctg ccgccgattc cccatttaca tcctgataat atgtttctag
   122101 cagcatgaaa ttgctgataa acaggataat ccatgatttt ctcttgtcta tacgattctt
   122161 gttcgatgat gtcccacgga tgaatgactt gtttgacggc atgttttgag atttctgttg
   122221 accatctctg attgcgggca ggtccacgta aaccaatcgg tagtatttct gcgtgatccg
   122281 tcgacgcacg acgaactgtt ccgaacatct tttcagttag aacgacccat tcaggcaagg
   122341 tgaacccttc tatttcatgt ttatcaaaaa ggacaatatc atgtgcttga atcatttttt
   122401 atgcatccca ttcttcgtcc attttggcac ggactttatt tgtttcgaca cgtccgaatg
   122461 ttttggctgt ttcgtttgtg tatcgatttg tcagcatcgt gtcatttgta ttgcggacat
   122521 cttgaatggc ttcattgatt gcttcgatga ctttgctgac attttgttct gaagcagctt
   122581 ggtcggtgat atcttctagg aaatgataga tcgctcctag ttttgtataa ttattgatat
   122641 cgtaagcgat agctggaact ttttcagcag ctgcttcgat ttcagcaacc gaacgttttg
   122701 tcacacgtgc agcactttct ttagacatcg cttggacggt aatttcttta ttgtttaaag
   122761 caatcaagcg gcttgattgt aatccatgtg ctaaaaatgc acctgaaata gcatttcctg
   122821 caatcaatcc aataactgga tgtccagctt gacggagttt tgcataagca gcagcactat
   122881 tagccaaagc cacatggata ccgatcaatt cttcgttata gccgtatgct tggcttggta
   122941 catcgatcac aacgatgatt ggccgtttca cttttttgtc tttatcttct tcgtaaacgt
   123001 gatccaaaat ttgtcccatg cggaaacctt caactaaacc aacttcaccg tttcgtacac
   123061 ggtacatcgg gtttttagga tcaggtacga ttgatgtcac gatggcttct tctatgaatt
   123121 tacttgattt accataatac acagtactaa tagaagaagt tggatttttc atgccagtta
   123181 gtgccgcaaa ccaatcgtaa ccaacacttg ctgcagctgg ttcatttcct tctgctactt
   123241 ctggtaagtc aattgatttt gcttgatggt tagcaattgc ttcattgtaa tcgtcaatgg
   123301 ccattggttt tgaaagatct aaagaatcaa gtaatgataa ataaaatgct gagctttcac
   123361 tgcgatgcgt gtcttttcgt tcgtccattg ctgcgatgac agcagctttg attgcttcta
   123421 cactatcagt cactaattca tcaatgattt ttgattctac ccgttgacga gatcctaatg
   123481 tattccaaat caagtctttg tcactagaat caaattctcg aacgcctgct tcttgttcaa
   123541 taacttctgg tccattcaaa ccgactctcg ctttttttgt tgcgattaaa taactcatta
   123601 atgctgaagt aatcgacata ccgccaaatg atcctacacg tcctggaatc aaaccgatga
   123661 ctggtacata ttgttttaga gcaatgatta catttccgat ttctgaaata gataacagtc
   123721 cgtagtttgc ttcttgcaaa cgaacaccac ctgtatctag tacaatgact ggaaagatct
   123781 catttccttt tttattttct tctaacacat gttcaagtgc agcaacgatt tttgcaccgg
   123841 aaacttcacc gataccgcca ccttggaatt ttccttccat agcgattact actgcttgtt
   123901 tgccattgat cgtgccacgg ctgacaacga tcccgtcatc actttcaggg acgattcctt
   123961 gtgctggtaa acttggcgcg atgatttgat cgaatggatc aatcaattca cgtccattgt
   124021 caagtagagc gttcactcgt tctctggcgt ttaattctac aaaactattt ttcatctgaa
   124081 agttcctcca ttgcttgcgt caatcgtagg tacacaactc ctggtgtagc accgaagtca
   124141 ttgatcacga tatctgctga aattggataa cgatcaaaaa atctcgttaa tacattgttc
   124201 catacttcgc cgaagccatc gcttcctgta caaattttta cagtagattc tgttttttct
   124261 gttggaaaca tcaagatttc caaatctcct gaaccgacca ctcctacatg gattttctta
   124321 ttgatcggct gtttcgttgg atatttataa gctaattgtt ccatggatct ctcccctttt
   124381 tgtaaaacag agcatacacg ttctgcttat tcgtctttct tcttccatct gggtaaatgg
   124441 cgacaaaagg tttttctcgc caattttatg accaagttct gaatttcatt ggtggttcat
   124501 acaggccatc tgaccattcg atcaaatcat cgatggtttg tgcagctaat aatgaacgtt
   124561 tcgcttcact acgtttgatg cctagatctt ctggtaattt cacgatgccg gctgcacgca
   124621 agctatctag tgattctttc gtgctagtca ttccaagagg tgtaacaccg ccaattgctg
   124681 caattgctgc ttgtcgttct tcttttgttt ttgctttgta caaataagca attccttctt
   124741 ctgtcacgat atgtgtagta tcttcgctat aaatcatcac tggtgcattg gctaagcctg
   124801 cttgtttttg aacagctagt gcatctaatt cttcgacaaa gactggtttt ttatttgaac
   124861 catatgtttc aaccatttga acaactaatt tttggccttt ccctaatgga tcatcattgt
   124921 cgcgaagtga ttgccatgct ggtgtagaat gacgacgacc gccaggatta tgtcccatgt
   124981 taggcgcacc gccaaaacca gataaacgtc cttttgttac ggtagaagag tttccttcgt
   125041 aatctaattg caaggttgaa ccgataaaca tatcgatggc atattgtcct gcagcttgtg
   125101 ataatgttcg gttactgcgc atcgtgccgt ctttaccgac aaaaaagaca tctgggcgag
   125161 cagcaatata tttttccata ccaacttcgc caccaaaact atggatgctt tcaacccaac
   125221 ctgattcgat ggctggaatc atcgttggat gtgggttcaa tacccagttc ttcgcgattt
   125281 tcccttttaa ccctagtgat tcaccataag ttggcagtaa caattcgata gctgctgtat
   125341 tgaatccaat accgtgattc aaagattgta cgccgtgttc tgcgtagatt cctttgattg
   125401 ccatcattgc cattagaatt tgcaattctg tgatgttttg aggatctcgt gtgaataaag
   125461 cttctaattg ataaggttta tctgctttca cgacaacgtc tacccagtca ccagggatat
   125521 ctacacgtgg taaagaatcg acaacttcgt tgacttgaac gatcacgata ccatctttga
   125581 aagctgcagc ttcgatgatt gttggtgttt cttccgtatt gaaacctgtg tacaagttgc
   125641 cgtctttatc tgctttatct gcagctacta aaacaacatt tggaatcaaa tcgatgaata
   125701 agcgtccata aagttccaaa taagtatgga tatcacctaa tttcattgtg ccgtcttcaa
   125761 tcatttgggc gacacgcaag ctttgtgcac cagcataaga gaaatcgatc ttgcttgcga
   125821 ttcctttttc aaaaatattc aaatgttctg gtcgtgagat actagacatg atcaaatgca
   125881 gatcattcac tttttctgca tctacttttt caagactttc tgaaagaaaa cttgcttgtt
   125941 tttgattgtc gccttctaat acaactttgt cgccgggagc aatcagtttt tccattactt
   126001 ctacgatttt ttcagtttct acatacttgc cattgagcaa gttgctgatt ttttccaatc
   126061 gctgcgcttt tttttcgcgt ttagaagccc agttgcggtt tggtttttcc ataattagtc
   126121 atctccattt ttgatataga aacgtggcgt catcaatacc atgttggtct agattgatcg
   126181 tacggatccc tttatctaca ccaaattttg tatgaatcag ctttgtcaaa gtattgccgg
   126241 ggggaaattc aatcgtcaga tccattccta attcttttgc caagtccatc atttggttcc
   126301 actgcacagg gaaagctagg ttttccaaca aatctttacg aatctcttct gcgtcctgag
   126361 tggctcttcc atccgtattt tttatataca aacatctagc atcagataga tggacagact
   126421 gcatatacgg cttgaatttc ttgaccgtat cattcatcaa gagacagtga gatggcacag
   126481 ggactttcag cagcttcgct gtttgcgctt ggtgccgttt ggcttctgcc aacgtaacgg
   126541 ataaaccttc gatactccca gataacgtat gttgcatcgg acaattttca tttgacagat
   126601 aaactggatg gtcaggatca aatgtttttg ctacaatttc ctcagcttcg cttctcgtca
   126661 cacctactac tacgcccatt gcataaccgc ttggataagc ttctttcatt aagactgcgc
   126721 ggtgatagac tagttccgct gcattctcaa atctcaaaga tcgacaagca acagctgcac
   126781 aaaaagcacc aatcgaatgt cctgctgcaa tatcaggctg aataccgttt ttttccattt
   126841 catctaaata aaatactgct tttgtcagta atgctagctg gataaaaacc gtatcttgat
   126901 agttttcttc tttatcaact aaccgatacc ccgtcacatt ttctaaacgt tccatgtaca
   126961 tttcagggat atccgctaac atccctaact tctgactgcc ttgtccagga aataagaaac
   127021 ctgtactcat gagcctcgcc tcctcagcac ttactttttc cactgctatc ttagtcaatt
   127081 tctgctataa taaaaaatgc caaaaaaaga aaaaaccata acaaaaaggt atggatggga
   127141 tggataatta tgaaattgct tcatctgcag tattttaaaa aagtattcga aacaaaaaat
   127201 gttacccaag cagccaaaga attatttatt tctcaacctg ccttgagccg tgcgatcaaa
   127261 catttggaaa acgaattagg tgtccctttg ttttaccata gcggcagaaa cattgagacg
   127321 accgtttatg cagaagaatt ttatccatat gcagtcaaga cattagacac attagaagaa
   127381 ggaatcaaat tattgtcttc ggtcaatgag aagatcgtta cttccgtcat attgtattta
   127441 gaggtagcct ctgtttctat tccgaacttg gtccgaattt ttatggaaaa gcatcctgat
   127501 atccaattaa cgatcatgca acatcattta ccagatgata ttaccgatcc tgtactgtac
   127561 atcacttcgg agatgaagcc tggcatgacc aatattccta tcatgaaaga acctgtttat
   127621 gtcgccattc ctaagaaaca tcctcttgct aaaaaagaac atctgcaatt agaagatatc
   127681 caacgcaccc ccatcttgat gctgtcaaag agcaacgcct tccgtcatac actggatgca
   127741 gcacttgaag cgaaaagtat cgaattgacg atcagttcga cgacagatga tcctgcgaca
   127801 ttacgttcta ttgtcaagca aggattagct gttagctttt tcccaaaagt ttcttggtct
   127861 tacgataaaa atgatccttt cgtcttgcgg gaaatcaaag atttgccctt gacacgaaca
   127921 atttatttat cttctagctt cacagaagat catcctttga tcaaaatgat tgcaaaaacg
   127981 atgcgggaat tttatttagg aaaataaaaa aaaaagagct gggacaaaat tcttgtcgca
   128041 gctctttttt cgaacaaacg gtgttccaga agtaatttct tagaaaataa gccgaaattt
   128101 cccaaaaatt tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag ctaaacattt
   128161 ctgtcacaac ctcttctcta ttgtcctaga cattcattgc gttgatttaa aacttccatc
   128221 aatcaaatct gctagttcat ctagcttttc tattttacgt agatcaatag agatccaatg
   128281 ttctttattc atgtgatagg ctggaaaata cccctctttt cctcgcaaaa ttccgccaag
   128341 ttcaggatcg accttcaaat caagaatatt gatgatccta tggtcacttg catcatcata
   128401 gattttttct tctggaacag acatcaggac cccaaaccat tttttgcttt tttgatgtct
   128461 gaatacgcaa taatcaggaa attttttaaa aatatattct ggagacaaat tgtatgtttc
   128521 attgatgtga tcgattagtt cttttttcgt tagcatctgc ttccatctct ttcttattga
   128581 ttcgctttat tcaaagatac cacgtaagaa tagaaaaatg aatacaagta gttgctaatc
   128641 aaatacttcg atatttttct tttttatcaa ctcttaaatg ttcaatccgc ctgtgttggt
   128701 cttgctacca tctactatta aaatgtttat tattgtttgc ccacctattt tttgatttta
   128761 gtggtatcat ttttcaagtt catgatgaaa ttcttatcat cagtaaatgc tccagaaaaa
   128821 taaacaatga ttgaaaaatc agttgaaact tgaccaccgt ttattttctc cgtattctta
   128881 gaatagaagg gagtctgatt gatgtattca ctaatgaaaa aaatcattac cgaaaaagat
   128941 attcgacgtc atgtttcttt agtagaacaa ttgctgaatc atacaaagat aaccgtaaat
   129001 gaattagctg aaatcatagg gacaaccgaa cgaacgattt tttctgacct tcaatctatt
   129061 cgctctcagc tgcccgaagg ctgggacatc atcagcgatc aagcaggaat tagtttgcaa
   129121 aaccagcaaa atttattaac aaatgacctt tgggagattt ttttcaaaca atcagtcagt
   129181 gtcgaactgc tgaaaaatct gctttttaca aaaaaagttg ctgtccctga ttttctagcc
   129241 gaccatgggc tttcttatgg gacattgaaa agacatgtga ctaaaatcaa tcagcgttta
   129301 gcaagttacg acttgcagat cgatttgacg aaatatacag cttgtattct tggaaaagag
   129361 cgtgcgattc gtacttttta tcatcgattg ctgattcctt tcactcataa caactatttt
   129421 ttcgaggatt attcgatcca tgaatcccat tattttcaat ttttaagaaa cctcagtcaa
   129481 actgaactag cggttgaaac agaagagatt ttcggaacat gttggttctt tatcaatacg
   129541 atccgtataa aagcgaattg ccgattagat tcttctatcc ataccaacag cacactctcc
   129601 tccctgtatg actctgcatt gaaaaaattg tatttaacgg aaggtattta tcttaaagac
   129661 actgagcttt cttttgcttc tttctgcttt ctggaaagtt ggaattacaa caataactat
   129721 ggacaagaaa ccgctcgctg cctgcatcat tctccatttt tagaggtcct cgaaacgttt
   129781 gtagaggaac tagcttcaga gctgtctctt gatcaactaa aaaaaacatc tctgactgat
   129841 aacttggctc tcctcatatt aaaatatcat gaatcgccga tattgattga agcattggac
   129901 cgtcaatacc atcgtttttt agaaacatat gatcagcaat atgcacactt gtatcaacag
   129961 aaagagaatt tactgaacga tctaaaaaaa gaaatagaaa tcaatgaacc aaattacttt
   130021 ctccaactct tgtctctact gatccaacaa acgatttttt ctatcaaacc gaatttgttc
   130081 aatgtctatt ttattttcca aggagaacct tcatggaagg cgttcttgca gcaggaatta
   130141 aaagattact taggaaaacg tgtctgcttc ttacctatcg aactaaccga tctttccact
   130201 atttcttttg aaaaaacaga tatcttgatt tccaatttcc ctctagattc tctagatatc
   130261 cctattgtat acatttcttc tattccaacg aaaaatgagc tcaatcgttt gactgaactc
   130321 actttcaaaa cttttttata gtaacggcta gcgactgtat tctctagctg ttttttcttt
   130381 tttgcgaaac agtttgttca tgatcccttt ttctatttct acaactaaga atagaagaat
   130441 cacatttgct ccaatcaata actgttgtgt catcgttaaa cttactgtac cgatcagctg
   130501 ctggccgact ggtagaaaaa taaccatccc ttgcaataaa gcaaggatac ctaatgaaag
   130561 aaaaagcgct ttgttttgca aaagaccttg gttcaatgac ggcttggaca actctctgca
   130621 attgatcatg taaacagctt gagctaagac gatattttgc agaagaatgg tttgctgtaa
   130681 gctttgccct tcaaactgca tagccaataa ataggcagga accatgatca gtagggagac
   130741 atagatgatc cgaaacaaat gataaccaga taggattcct tgctttggat ctcttggtgg
   130801 gcgcttcatg atttctggat ctggcgactc gaaccctagt tcataagata aagtgattgt
   130861 cgttaccata ttgacccata aaatctgtac tggggacaaa ggtagcgggc gattcagcat
   130921 aagtgcccaa atcaagatca atccttgagc taatgcagtt ggcaagaaga aattgatggt
   130981 tttttgcaaa ttatccaaaa tccgccggcc ctcttttacg gcttttgcaa tcgtatggaa
   131041 attatcatct cctaaaacca tatcagctgc ctgtttgctt acttcactac ctttgattcc
   131101 catagcaatt cctacatcgg atcttttcaa agcaggtgca tcatttacgc cgtctccggt
   131161 cattccgacg atttctccat ttttctgcaa agctttaaca attcgcagtt tatgttctgg
   131221 tgtcgttcga gcaaagacat cgactattgg cacttgctga atcaactctt catcagacat
   131281 aagatccaaa tcgatccctt ctaaaacttt ggcagtatgt tttaagccaa tttgttccgc
   131341 aatcgctttt gctgtgtctt tgtgatcccc tgtaatcatt ttcactgaga tccctgcttg
   131401 ttggcattcc tttacagctt tgactgcact ttcttttggc ggatcgatca atccggcgat
   131461 ccctgcaaaa gttaattggg ttaaagtctc atgcgtgagt tcctgtttcg aatctacttt
   131521 tttataagca aatcctaaaa cacgctgacc ttttctcgca aattctgcag ctgtctgact
   131581 ccatgcttct tgttctgcag aagaaagagt tgatttagca aataatactt ctggtgcacc
   131641 tttgacgtaa atcaccgctc ctttttcctc cgcatgcatc gttgccatat atttgtattc
   131701 agaactgaac ggaatctttg cttctatttt tttcaaaggt agattggctg tttctgtgtg
   131761 tcgaatcagc gcaacttctg tcggattgcc acttaaattt tctgttttct gttcttcttt
   131821 tgtttttaat tcctgacagt ttttcataat ggacaacacg caggcttctt cttttgcagc
   131881 tacatccaca actgtcattt cattttttgt caatgttcct gtcttatctg aacagatcac
   131941 tgtcatagaa cctaatgttt ctaccgaagg aagtcctttg atgatcgcct tttcttttgc
   132001 catttcatgt actccaacgg atagaatcat ggtcaaaaca gctggcaatc ctttgggtac
   132061 cattgacacc accaaagcaa tcgtagccga aagaaggata tgccaatcca taccatatcg
   132121 aaatgtcgta aaaataacta agaataggct caaacaaaga atgccttgaa aaatttgttt
   132181 gtttaatcgg ttgatttttt taatcagagg cgtcgtttgt tgttcgaccg attgcaatgc
   132241 ttgattgatt ttaccgattt ctgtcgtgtt tcctgtttct acaacgacac cgactgcaga
   132301 accagattgt acaagcgttc ctgaaaaagc catatttttc tggtcgccga tcgattcttc
   132361 ttctgtcaaa acggcatgac ttttttcaga tgcttctgat tctcccgtca aaatcgattc
   132421 ttccactaat aaatcatgtg cttcgattaa tcggatatct gctggaacga catctccggc
   132481 acgtaagaca atgacatctc cttttaccag ttctttcgca gggatttttt cttgcatccc
   132541 gtttgatagt actatcgctt cttgtcccat cagctgcttc aaaccattca atgattcttc
   132601 cgcttttctt tcttgccagt aactaacgag tccattgacg atcactacga ataaaataat
   132661 acttccttca atatattcac tagaagcaaa tttcaaaaaa gaggcaatga tcaacacgat
   132721 catcagcaga tcagtaaaat gctttgctat ttttttccat cgttttgtct gctgccttgc
   132781 ttttatttca ttatatcctt gatcagtcag gcgtttctgt cgttcttctt gcgacaagcc
   132841 ttctgttgat gtttgatagt ttttcaatac atcttctacc gtcaatctgt accagttcat
   132901 cctatcccct cctgtttaat tgatactgtt attatacgca gggaagtttt ttcaggcgta
   132961 tcgattattt caggggggtc tgaaataatt cgttttgcaa aatgtatctg ggcctaactg
   133021 atatttctat tgcttattct tttatataca aaatttaaga atacaaacca gataaccaat
   133081 aaaaaaggaa taaagcgtgt cgtgctttcg aataacaagc tgaaagcaat tgaagtaaaa
   133141 aacaccaaca aagcaagatc tttatatctt tgtgtagtgt tccgtttctt ttctttaata
   133201 aattgaacat gtgcacacaa gatcgaagac cacacaaata gaaaacaact cgttgtcatg
   133261 cctgccaaaa gctgaaaaaa caatcgacta tcagctacaa aaaaatgtag acaaagaccg
   133321 gcaaaaaata acattcctgt aattaaaata ccgtgtaaag gtacgccttt cttagaaagc
   133381 gcacaaaacc aatggggcaa acttttttct tttgctgttt gatataaaat cctgctagta
   133441 ctgtagactg cactatttgc tgaggacaaa gaagcaacca aaatcattag attgacaatt
   133501 gctgctccgc cttggcaatg gatcacttgc agcatagtca ctaaaggact ttcttttaaa
   133561 tctaaacaat gccatggaac aacaaataaa atcgacaaaa tcgttcctat atagaaaaag
   133621 agaatacgcc aaggcagaca gttgattgct tttttcaaat tcttttgcgg atcacttgtc
   133681 tcgccaacgg ttaagccgat catttcaatt ccagcaaaac tgaataaaac cataggaaat
   133741 gagactacca ttccagcaaa gccaaacggt agtaattcat cgatctgtcc aaaattttct
   133801 ttgattccga tagactgggt atgaaaatct gagacactca atcctatacc caataagata
   133861 aaaaggaaaa tccccaagat cttgatgatt gagaaaaaag attccaattc accaaaccac
   133921 ttgactgcaa caaggttgac tgccattaag atcattagtg cgctacctcc gcttatcaaa
   133981 gctggtacat gtacaaacca gaaatggata tacaatccta ttgctgttaa ttccgcaagt
   134041 ccagctccaa gccagcaaag ccaataagtc caattgacaa aaaatgccca ctttttgcct
   134101 aaataatagt caacgaaatc actgatagag ttaaatgtac tatttgctaa aaataaatca
   134161 cccatggccc gcatcaaacc ataaatagcc aaaccgcata tcaaataaac gagaataaca
   134221 gctggccctg ctgatgcaat tgcctttgct gaacctaaaa ataatcctgt tccaattgcg
   134281 ccacctaatg cgattaactg gatatttcga ttagttagag tccgtttcat tgccggtttt
   134341 tccatcaagt tcaccttttt ctttttcaag cagcatggcg acttcctgtt cccaagaagg
   134401 tttctttatt ttttcaatac agagtggtac tacaaacaaa atgacactga tggctgtgac
   134461 tgcaattaga ttgatccatg ttccagcttt catcgtcagt ccgacagaaa atccgattgc
   134521 cgcaaataaa acaataacga cagtccaaac tccaagattg ctctttttct ctttttttcc
   134581 aattctaaat ggtcgattca actttgcatg tgtataacgc aatctcaaaa ctgcgatccc
   134641 catgatgaca tagacgatac agtagataac agtagttgaa tttacgagca tcaaaaatgc
   134701 ttgattgacg ccaggaatca gcaaataaaa aagtgcaaag attgagataa tgacagactg
   134761 agtcaaaata acggatttgg atacgtcaaa ttgattcgtc ttccaaaaat tgaattttgg
   134821 tggatacgca ccttttctag cactttctgt cactgtcttt gctggtcctg aggcccaagc
   134881 agataattgg actgctacac caatgaagac aagtaaacta aacagattca caataagatg
   134941 tggcaaacca agaatttggc aataaatcga aatagattgc gcgatattat tcaattccat
   135001 ttgtcctttt ggtacgacat ttgctacaac taaagcattc aaaatattga cgaaaaatat
   135061 aaaaattaac gcggcaaata ctccgcgaat atacgtttta actggatttt ccaatcgagt
   135121 gatatacaca gaggacattt cgataccagt aaaaataaac atgatcggcg caaaataaac
   135181 aaatgaggat gcagtcgtag tatctggtat taatttgtcc catgaaaaac tgcctaatgt
   135241 tccggttggt acgatcccaa ctttgatcca cgcagcaaac cctaataaga gcatcatcgt
   135301 caaaggcacg tacaatccta accaaacacc gacttttccg ccgatttttg ccatatcaaa
   135361 tttcaaattc aaaaaagtga tgatccaata aacgactaaa atgacaacta atgtaaattt
   135421 gctgttcaaa ccaagcggca cattatcgat aatattacca aacaacggag cgagtgctga
   135481 agcgaccatc accatcccag gaaacatttg gacccataac agccatgaga caacgaaccc
   135541 ccattttttt cctagagcat tcgataccca aagttctgga ccaccttttt ggggaaacat
   135601 tccagcaaat tcaccagaga tcaatgagat tggaaaagcg tacaataata ctgcaaccag
   135661 catgtaaaaa atcatcgtcc agctcgttgc tgctacatct ggaatatttc gtacacttgc
   135721 tacaagggcg caggtcattc cgataaaggt catggtcgtg atttgctttg atgaaccagt
   135781 atctttcatc tttattcctt ctttcaatag cgacatatgt ttttgttttc ttttattttt
   135841 gttcaagaac agaaatgaca aggtttcatc gattcagtac aaaacccagt atcaccgaaa
   135901 catccattga acgatcttag tgaactgggt ttgatacatg aaacgaacga tccaacttat
   135961 agaacgtttt ataaaaaaga atatcttggc gaggaatata tgaaaaacag ggggaaatac
   136021 aattgttttg atgaagcaaa taattgtctg ttctccatat gtctcttgtt attctttcac
   136081 acaaaaatca gttctattgg atgatttcgt ttagtttctt ttccattgct ttggcgattt
   136141 tttttgtata aaaattgaaa attcgatcat cattcaaata aggagtcatg atagccgcac
   136201 gtagcagagt gattttctgt tcttttttcc attcttcttt cgttagaccc attctttcaa
   136261 caaaacgaat cggagaatca ccgtaatctt cttgtgtaaa tgtagtatga gaagtaataa
   136321 accgttcacc ataaacatcg ccatccatat aagaagaccg atcgaacatt ttttcattta
   136381 attcattgat agcgttcaaa tctgtacagc cttgttcttt gaatacccaa ttgaccatat
   136441 taaaatcagg atgattcaat ggatagactt cgattgtttt tcctttaaca gtaaaagtca
   136501 gatgatcgag aaattctctg aatctttgag ctgcctcgat tgaagcccca atcaattgac
   136561 catatccggt cacattcaat ggcaatacac gatgtgctgt ccagactgcg gcggcggttg
   136621 ctccagcttt tgatccttcc aaaatataag ctcctaacat atctggagcc tttactgatt
   136681 tttcaaacac atatggcgcg aaataagaaa tgatatttct catattttta tgtttgatta
   136741 cgattcctcc agcagcataa gggacatagc ccatcttatg aggatctatc gtgacagaat
   136801 ccgcttctga aatagctctg aacccttcat atacctcttt gtcaatcgtt acgcagtggt
   136861 ggaaaacatg atgttcttca aagaattcag ctaaggaagc atacggcaca aattctcctg
   136921 cttcgtttag aaacagtgaa cgcgcatagc cgccatatgc ggcatcaaca tgtaaataga
   136981 aatagatccc ttcatctttt aacctttctc ttaattggac gattttatct acactgtcta
   137041 cttgaccttc ttctgtcgtt cctacgacag caaccacacc tagaattggg attttttgtc
   137101 cagctaattc acgaattgtt ttttccaaag cattaatatc cattcgataa tcctcttgaa
   137161 ctgggatagc aaccatctga tctaagccga ctccgcaaat atctaacgcc ttcatccaag
   137221 aatagtgttt tgtttgaggg accagccatt tccctaatct ttggatattt tttccgctac
   137281 gggaagaagc tgctttgacc tcatccattt cttcgtccgt aaagctttcg gtcatttcca
   137341 aaatttcttc aacagataaa ttcaaaagtt cccactcaga catttttttc actttttcag
   137401 gcagcacttc cttgacagct aaaggaatcg atttgatgca acgggcgtac cataatcctt
   137461 caagattagc aatagaacca tctgctgcaa tgtgtcccca tccgtctgtc atattgaata
   137521 atgaagcaaa atcctgtcct acttctgctt ccatttgcga agttgccata gaagattcca
   137581 atgcaacatt atttgggttc cacagcatcg catagttgta agctaataaa gcaggcatca
   137641 atgtttcagc attcatttgt ccccagtaac ggcctgctga atgccatggg atcgatcctg
   137701 cgcgtaatcg ctgactgact tcatccaaga cctctatcat atggtcgcgg gtggctagat
   137761 aatctggagt gagcttatct ccttcgctaa ttgcaggcat atctgaagga atataatttt
   137821 ctctccatcc aagatgttca tctaccatct tgttcaacaa cattttataa actggtccat
   137881 tttcagcttt gtcgcctata aagacggcct tgatatccat atctttcatt tttcttccct
   137941 ccatattttg ttggtcaatg tcttgcagat acatgtgttt atctgtaaca cctcttcact
   138001 atagcgccaa aaatgagaaa ttttttttta taacatcaaa tccacgtaga tttttacaaa
   138061 caaaaataaa taaagtcgga tttttctgat aattcaacgc aaaaaagcga acaatttttc
   138121 aataaatcag tcttctaaaa tagataacct aaaaaaagcg aacaaacaaa taaaatgact
   138181 gtccttcatt cactaaacat atttttattt tt
//
LOCUS       DACDEG010000002        84259 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.002, whole genome shotgun sequence.
ACCESSION   DACDEG010000002 DACDEG010000000
VERSION     DACDEG010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 84259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.002"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            205..1197
                     /locus_tag="IV142_000119"
     CDS             205..1197
                     /locus_tag="IV142_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8483810.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            1213..2097
                     /locus_tag="IV142_000120"
     CDS             1213..2097
                     /locus_tag="IV142_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8483811.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            2146..3753
                     /locus_tag="IV142_000121"
     CDS             2146..3753
                     /locus_tag="IV142_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8483812.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            3754..5238
                     /locus_tag="IV142_000122"
     CDS             3754..5238
                     /locus_tag="IV142_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP8483813.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            5415..6407
                     /locus_tag="IV142_000123"
     CDS             5415..6407
                     /locus_tag="IV142_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8483814.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETIEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            6459..7328
                     /locus_tag="IV142_000124"
     CDS             6459..7328
                     /locus_tag="IV142_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8483815.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(7748..9313)
                     /gene="guaA"
                     /locus_tag="IV142_000125"
     CDS             complement(7748..9313)
                     /gene="guaA"
                     /locus_tag="IV142_000125"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP8483816.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9372..10295)
                     /locus_tag="IV142_000126"
     CDS             complement(9372..10295)
                     /locus_tag="IV142_000126"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP8483817.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            10491..11096
                     /locus_tag="IV142_000127"
     CDS             10491..11096
                     /locus_tag="IV142_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8483818.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11404..12420
                     /locus_tag="IV142_000128"
     CDS             11404..12420
                     /locus_tag="IV142_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8483819.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            12561..13862
                     /locus_tag="IV142_000129"
     CDS             12561..13862
                     /locus_tag="IV142_000129"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP8483820.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            13908..14570
                     /gene="deoC"
                     /locus_tag="IV142_000130"
     CDS             13908..14570
                     /gene="deoC"
                     /locus_tag="IV142_000130"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP8483821.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            14591..14989
                     /locus_tag="IV142_000131"
     CDS             14591..14989
                     /locus_tag="IV142_000131"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP8483822.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15055..16137
                     /locus_tag="IV142_000132"
     CDS             15055..16137
                     /locus_tag="IV142_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8483823.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            16349..17911
                     /locus_tag="IV142_000133"
     CDS             16349..17911
                     /locus_tag="IV142_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8483824.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            17904..19037
                     /locus_tag="IV142_000134"
     CDS             17904..19037
                     /locus_tag="IV142_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8483825.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19037..19993
                     /locus_tag="IV142_000135"
     CDS             19037..19993
                     /locus_tag="IV142_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8483826.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20092..21258
                     /gene="deoB"
                     /locus_tag="IV142_000136"
     CDS             20092..21258
                     /gene="deoB"
                     /locus_tag="IV142_000136"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP8483827.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21282..22100
                     /locus_tag="IV142_000137"
     CDS             21282..22100
                     /locus_tag="IV142_000137"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP8483828.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22117..22824
                     /gene="deoD"
                     /locus_tag="IV142_000138"
     CDS             22117..22824
                     /gene="deoD"
                     /locus_tag="IV142_000138"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP8483829.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23122..23808)
                     /gene="gpmA"
                     /locus_tag="IV142_000139"
     CDS             complement(23122..23808)
                     /gene="gpmA"
                     /locus_tag="IV142_000139"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP8483830.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(23980..24561)
                     /locus_tag="IV142_000140"
     CDS             complement(23980..24561)
                     /locus_tag="IV142_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8483831.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(24694..25377)
                     /gene="rpiA"
                     /locus_tag="IV142_000141"
     CDS             complement(24694..25377)
                     /gene="rpiA"
                     /locus_tag="IV142_000141"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP8483832.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            25696..26109
                     /gene="rpsL"
                     /locus_tag="IV142_000142"
     CDS             25696..26109
                     /gene="rpsL"
                     /locus_tag="IV142_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP8483833.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26193..26663
                     /gene="rpsG"
                     /locus_tag="IV142_000143"
     CDS             26193..26663
                     /gene="rpsG"
                     /locus_tag="IV142_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP8483834.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            26757..28844
                     /gene="fusA"
                     /locus_tag="IV142_000144"
     CDS             26757..28844
                     /gene="fusA"
                     /locus_tag="IV142_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP8483835.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29016..30203
                     /gene="tuf"
                     /locus_tag="IV142_000145"
     CDS             29016..30203
                     /gene="tuf"
                     /locus_tag="IV142_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP8483836.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            30610..30918
                     /gene="rpsJ"
                     /locus_tag="IV142_000146"
     CDS             30610..30918
                     /gene="rpsJ"
                     /locus_tag="IV142_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP8483837.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            30949..31578
                     /gene="rplC"
                     /locus_tag="IV142_000147"
     CDS             30949..31578
                     /gene="rplC"
                     /locus_tag="IV142_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP8483838.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            31606..32229
                     /gene="rplD"
                     /locus_tag="IV142_000148"
     CDS             31606..32229
                     /gene="rplD"
                     /locus_tag="IV142_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP8483839.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            32229..32519
                     /gene="rplW"
                     /locus_tag="IV142_000149"
     CDS             32229..32519
                     /gene="rplW"
                     /locus_tag="IV142_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP8483840.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            32558..33391
                     /gene="rplB"
                     /locus_tag="IV142_000150"
     CDS             32558..33391
                     /gene="rplB"
                     /locus_tag="IV142_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP8483841.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            33434..33712
                     /gene="rpsS"
                     /locus_tag="IV142_000151"
     CDS             33434..33712
                     /gene="rpsS"
                     /locus_tag="IV142_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP8483842.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            33735..34082
                     /gene="rplV"
                     /locus_tag="IV142_000152"
     CDS             33735..34082
                     /gene="rplV"
                     /locus_tag="IV142_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP8483843.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            34096..34752
                     /gene="rpsC"
                     /locus_tag="IV142_000153"
     CDS             34096..34752
                     /gene="rpsC"
                     /locus_tag="IV142_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP8483844.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            34755..35189
                     /gene="rplP"
                     /locus_tag="IV142_000154"
     CDS             34755..35189
                     /gene="rplP"
                     /locus_tag="IV142_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP8483845.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            35179..35367
                     /gene="rpmC"
                     /locus_tag="IV142_000155"
     CDS             35179..35367
                     /gene="rpmC"
                     /locus_tag="IV142_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP8483846.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            35392..35658
                     /gene="rpsQ"
                     /locus_tag="IV142_000156"
     CDS             35392..35658
                     /gene="rpsQ"
                     /locus_tag="IV142_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP8483847.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            35716..36084
                     /gene="rplN"
                     /locus_tag="IV142_000157"
     CDS             35716..36084
                     /gene="rplN"
                     /locus_tag="IV142_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP8483848.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            36121..36429
                     /gene="rplX"
                     /locus_tag="IV142_000158"
     CDS             36121..36429
                     /gene="rplX"
                     /locus_tag="IV142_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP8483849.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            36457..36996
                     /gene="rplE"
                     /locus_tag="IV142_000159"
     CDS             36457..36996
                     /gene="rplE"
                     /locus_tag="IV142_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP8483850.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            37015..37200
                     /locus_tag="IV142_000160"
     CDS             37015..37200
                     /locus_tag="IV142_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP8483851.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            37236..37634
                     /gene="rpsH"
                     /locus_tag="IV142_000161"
     CDS             37236..37634
                     /gene="rpsH"
                     /locus_tag="IV142_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP8483852.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            37666..38202
                     /gene="rplF"
                     /locus_tag="IV142_000162"
     CDS             37666..38202
                     /gene="rplF"
                     /locus_tag="IV142_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP8483853.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            38363..38719
                     /gene="rplR"
                     /locus_tag="IV142_000163"
     CDS             38363..38719
                     /gene="rplR"
                     /locus_tag="IV142_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP8483854.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            38740..39240
                     /gene="rpsE"
                     /locus_tag="IV142_000164"
     CDS             38740..39240
                     /gene="rpsE"
                     /locus_tag="IV142_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP8483855.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            39255..39434
                     /gene="rpmD"
                     /locus_tag="IV142_000165"
     CDS             39255..39434
                     /gene="rpmD"
                     /locus_tag="IV142_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP8483856.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            39478..39918
                     /gene="rplO"
                     /locus_tag="IV142_000166"
     CDS             39478..39918
                     /gene="rplO"
                     /locus_tag="IV142_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP8483857.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            39918..41213
                     /gene="secY"
                     /locus_tag="IV142_000167"
     CDS             39918..41213
                     /gene="secY"
                     /locus_tag="IV142_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP8483858.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            41275..41922
                     /locus_tag="IV142_000168"
     CDS             41275..41922
                     /locus_tag="IV142_000168"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP8483859.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            42113..42331
                     /gene="infA"
                     /locus_tag="IV142_000169"
     CDS             42113..42331
                     /gene="infA"
                     /locus_tag="IV142_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP8483860.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            42364..42480
                     /gene="rpmJ"
                     /locus_tag="IV142_000170"
     CDS             42364..42480
                     /gene="rpmJ"
                     /locus_tag="IV142_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP8483861.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            42498..42863
                     /gene="rpsM"
                     /locus_tag="IV142_000171"
     CDS             42498..42863
                     /gene="rpsM"
                     /locus_tag="IV142_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP8483862.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            42891..43280
                     /gene="rpsK"
                     /locus_tag="IV142_000172"
     CDS             42891..43280
                     /gene="rpsK"
                     /locus_tag="IV142_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP8483863.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            43360..44298
                     /locus_tag="IV142_000173"
     CDS             43360..44298
                     /locus_tag="IV142_000173"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP8483864.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            44326..44706
                     /gene="rplQ"
                     /locus_tag="IV142_000174"
     CDS             44326..44706
                     /gene="rplQ"
                     /locus_tag="IV142_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP8483865.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            44978..45817
                     /locus_tag="IV142_000175"
     CDS             44978..45817
                     /locus_tag="IV142_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8483866.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            45793..46662
                     /locus_tag="IV142_000176"
     CDS             45793..46662
                     /locus_tag="IV142_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8483867.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            46652..47449
                     /locus_tag="IV142_000177"
     CDS             46652..47449
                     /locus_tag="IV142_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP8483868.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            47652..48398
                     /gene="truA"
                     /locus_tag="IV142_000178"
     CDS             47652..48398
                     /gene="truA"
                     /locus_tag="IV142_000178"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP8483869.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(48853..51522)
                     /locus_tag="IV142_000179"
     CDS             complement(48853..51522)
                     /locus_tag="IV142_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP8483870.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(51760..51948)
                     /locus_tag="IV142_000180"
     CDS             complement(51760..51948)
                     /locus_tag="IV142_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP8483871.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            52218..52580
                     /locus_tag="IV142_000181"
     CDS             52218..52580
                     /locus_tag="IV142_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8483872.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            52632..54314
                     /locus_tag="IV142_000182"
     CDS             52632..54314
                     /locus_tag="IV142_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP8483873.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            54431..56467
                     /gene="recG"
                     /locus_tag="IV142_000183"
     CDS             54431..56467
                     /gene="recG"
                     /locus_tag="IV142_000183"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP8483874.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            56516..57517
                     /gene="plsX"
                     /locus_tag="IV142_000184"
     CDS             56516..57517
                     /gene="plsX"
                     /locus_tag="IV142_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP8483875.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            57639..57881
                     /gene="acpP"
                     /locus_tag="IV142_000185"
     CDS             57639..57881
                     /gene="acpP"
                     /locus_tag="IV142_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP8483876.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            58149..59153
                     /locus_tag="IV142_000186"
     CDS             58149..59153
                     /locus_tag="IV142_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8483877.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            59154..60098
                     /locus_tag="IV142_000187"
     CDS             59154..60098
                     /locus_tag="IV142_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8483878.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            60098..61060
                     /locus_tag="IV142_000188"
     CDS             60098..61060
                     /locus_tag="IV142_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8483879.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            61087..62001
                     /locus_tag="IV142_000189"
     CDS             61087..62001
                     /locus_tag="IV142_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8483880.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            62027..63808
                     /locus_tag="IV142_000190"
     CDS             62027..63808
                     /locus_tag="IV142_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8483881.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            64156..64842
                     /locus_tag="IV142_000191"
     CDS             64156..64842
                     /locus_tag="IV142_000191"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP8483882.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            64862..68443
                     /gene="smc"
                     /locus_tag="IV142_000192"
     CDS             64862..68443
                     /gene="smc"
                     /locus_tag="IV142_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP8483883.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            68452..69261
                     /locus_tag="IV142_000193"
     CDS             68452..69261
                     /locus_tag="IV142_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8483884.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            69272..70270
                     /gene="ftsY"
                     /locus_tag="IV142_000194"
     CDS             69272..70270
                     /gene="ftsY"
                     /locus_tag="IV142_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP8483885.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            70457..72724
                     /gene="gshAB"
                     /locus_tag="IV142_000195"
     CDS             70457..72724
                     /gene="gshAB"
                     /locus_tag="IV142_000195"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP8483886.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKDR
                     RLTMNVLKLLYPEVF"
     gene            complement(72813..73265)
                     /locus_tag="IV142_000196"
     CDS             complement(72813..73265)
                     /locus_tag="IV142_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP8483887.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            73415..74365
                     /locus_tag="IV142_000197"
     CDS             73415..74365
                     /locus_tag="IV142_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP8483888.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            74358..75317
                     /locus_tag="IV142_000198"
     CDS             74358..75317
                     /locus_tag="IV142_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP8483889.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            75314..76069
                     /locus_tag="IV142_000199"
     CDS             75314..76069
                     /locus_tag="IV142_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8483890.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            76092..77045
                     /locus_tag="IV142_000200"
     CDS             76092..77045
                     /locus_tag="IV142_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8483891.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(77689..77985)
                     /locus_tag="IV142_000201"
     CDS             complement(77689..77985)
                     /locus_tag="IV142_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP8483892.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            78340..78696
                     /locus_tag="IV142_000202"
     CDS             78340..78696
                     /locus_tag="IV142_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8483893.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            78706..79605
                     /locus_tag="IV142_000203"
     CDS             78706..79605
                     /locus_tag="IV142_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP8483894.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            80122..80853
                     /locus_tag="IV142_000204"
     CDS             80122..80853
                     /locus_tag="IV142_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8483895.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            80998..83025
                     /locus_tag="IV142_000205"
     CDS             80998..83025
                     /locus_tag="IV142_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP8483896.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            83133..84230
                     /locus_tag="IV142_000206"
     CDS             83133..84230
                     /locus_tag="IV142_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP8483897.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT        27456 a        15038 c        18215 g        23550 t
ORIGIN      
        1 gcagaaaagg gtgcttaaaa ataatctgtt gtttaaaata ttattatcag aatacttaga
       61 tacaaaattc tggcacaaag taaaaaaaat attgacttaa aaaaatggat atggtaatct
      121 ataaacgaaa atagtggtaa cgtttccaaa atgcatcttc gttaaatttt tattttttta
      181 aacgaaaatg gaaacgtttc taaaatgtat tgggaggaga ttgaattgga aaagacattg
      241 ggaccaggtg gtgagataag ggcggtacaa cgggataaaa aaaatatttc gaagtgggaa
      301 aaattaaaga aacagttgcg gcgtgataag tggatgtact tgttgctctt accaggtatc
      361 atcatgacgt ttatctttaa gtatgtgcca atgtacggtg ctgtgattgc atttaaagac
      421 tataatccta tgctaggaat tttggaaagt ccttgggtag gactagatca ctttagggaa
      481 tttatcagtt caccaaattt tacgcttttg ctgagcaaca cattaaaaat tagtatttat
      541 ggtttattgt taggtttttt tccaccaatt attttagcac tgagcttgaa tcaaattatc
      601 aataaaaaaa taaaacagaa aatccagttg attctatatg cgccgaattt tatctcagta
      661 gttattattg tggggatgct gtttttgttc ctatccgcaa atggacctat taatgggtta
      721 gtagaaaagg ttacggggca accaattatg ttcatgtcta atccagctta tttcagaacg
      781 atttatattt tatcgggaat ttggcaagga atcggctggt catccattct ttacaccgca
      841 acgctggcgg gagtcagtac cgatttatat gaggcagcaa atatcgatgg ggcaaatatt
      901 ttacaaaaga tttggcatat cgatttgcct gcattgaggc ctgtgatggt gatcagcttc
      961 atcttggccg ccggcggtat tatgaatgta ggatatgaaa aagcctactt gatgcaaact
     1021 tcaatgaaca tccctgcctc agaaatcatt tccacttatg tatacaaagt cggcttgcaa
     1081 tcaggtgatt atgcctactc tgctgctgtt ggtctgttca atatgattat taatgtcgtt
     1141 ttattactga ttgtcaacaa tataaccaag cggctaaacg atggtcaagg cttgtaatga
     1201 aaggagaaaa ttatgaatag tgctttatca aaaatcgacc gtcaaatctt atggttgaat
     1261 cgagtgattg taactttttt agtcgtcatc accattgttc cgctgattta tgtactggta
     1321 gcttctttta tggacccagc tactttgatc aataaaggta taagcttcaa tccgaaagat
     1381 tggacaatac agggatatca acgggtattt gcagatgatt ccattttacg cggttttgta
     1441 aattcgttgt tttactcttt tagcttcagc ttattgactg tatttattac gatgatcact
     1501 gcgtatcctt tgtctcgacc taacttggcg gggaaaaaga ttattatgac tttttttgtc
     1561 attacaatgt tcgtgggtgg tggcttgata cctacctatt tattggtaaa gaacttgggc
     1621 atgttagata gtgtatgggc gattatcgta ccaggtgcta taaatgtgtg gaatattatc
     1681 ttagcccgta cctattttag tgctttgcca aaagaacttt ctgaagctgc tactatcgat
     1741 ggcgccaatg acttgcaaat actgttcaaa gtcatgcttc cattagcgaa accaattatg
     1801 ttcgtattgt ttttatatgc ctttgttggg caatggaact catactttga tgccatgatt
     1861 tacatcaaag atcccgactt acagccgttg caactcgtat taagaaatat tttgatccaa
     1921 aaccaacctt cacaagacat ggtgggagcc aatactgcca tggcagaaat gaaacaaatt
     1981 gctgagttga tcaaatacgc aacgatcgtc atttctagtc tgccattaat cataatgtat
     2041 cctttcttcc aaaaatattt tgataaaggt gttttagttg gttctgttaa agggtaagta
     2101 ttgttcacta tttgtatgaa taaaaattat aaaggagatt ttaagatgaa agcaaaatgg
     2161 atgctgccaa tcgtttttac ttccgtacta attttggggg cttgtggagg ctcaaaagaa
     2221 aagagtaaat caagccctga ttatgaacta gaaaatgtct cttttccatt gaaagaaaaa
     2281 gtatctttaa aaatgatgtc tcaaagtgcc cctttggctc ccaatgatcc taatgacaaa
     2341 ttgattttcc aacgattaga agaagagacc aatctccata ttgattggac aaactacagt
     2401 agtgattttg ccgaaaaaag aaatttggat atcgctagtg gtgatttacc agatgccatc
     2461 ttcaacgccg ctgcaggtga ctatgattta ttaaactggg cggaaagtgg cgttattgta
     2521 ccgattgaag atttgatcga agattatatg ccaaatctga agaaaatttt tgatgaaaac
     2581 cccgaatacc gacaaatgtc tactgcacct gatggtcaca tttactctat gccttggatt
     2641 gaagaattag gcgaaggaaa agagtcgatt catacagtaa atgggatggc ttggatcaac
     2701 aaggagtggt tggataattt gggtcttgaa atgccgcaaa ccactgatca gttgatgact
     2761 gtgttggagg ctttcaagac gcaagatcca aatggtaatg gtgaagcaga tgaaattcct
     2821 tttagcttta ttaacaatgg aggaaacgaa gacttaaaat ttttaatggg ggcattcggt
     2881 ctaggtgata atgatgacca cttagtcgtt gataacgatg gtcaggtaca atttactgct
     2941 aacaaagaag ggtataaaga agccatcaaa tatttcaatg aaatgaataa taaaggtctt
     3001 attgataaag aggctttcga acaagattgg aatacgtatg ttgccaaagg gaaagaacat
     3061 aagtatggcg tgtactttac ttgggataaa gcaaatatca cgggtgcaga tgatagttat
     3121 gacgtcttgc cagtcttagc gggaccagat ggaacgaaac atgttactcg tacgaatggc
     3181 atgggtttct caagagatcg tttcgtaatt actagtgcga ataaaaactt ggaacttaca
     3241 gctaggtgga tagataaaat gtatgaaccg ttacagtctg tccaaaataa ctggggaacc
     3301 tacggtgatg aagcacaggc gaatattttt gaattaaagg atggtatgtt aaaacatttg
     3361 cctttagagg ggacagcacc gggggaacta agacaaaaaa ccgaagctgg aggaccatta
     3421 gcagttcttg attcttatta cggcacagtc actactatgc cagatgatgc gaagtggcgt
     3481 ttagatttga tgcatgacac gtatttgcca tatattacag aagacaatat ctatccacgt
     3541 gtctttttag aaaaagaaga aacagatcga ttagcaaaaa tcgaaacgga tatgtgggat
     3601 tacatctatc gcaaaagagc agagtggatc accggcggta aaatcgatga agaatgggaa
     3661 gaataccttg ctgaattgga acgtgtaggt ttggaagaat ggattcaaat aaaacaaact
     3721 ggctatgata attttttgaa gggagcacaa taagtgttag tagaagaaac ggcgattttg
     3781 gataaagcaa atcgatttat tgaaatgatg cgaggaaaag ttaaccctca gtatcgacaa
     3841 aactatcatt tttccgcacc catcgggtgg ataaatgacc ccaatgggtt tatatattac
     3901 aaagggcatt accatctctt ctatcaatac tatccttacg atagtcgatg ggggcccatg
     3961 cattgggggc atgcgcgcag tagtgatttg gtccattggg aagatctacc agtagcgtta
     4021 gccccagatc aaccttatga cagcggcggg tgtttctcag gaagtgcaat tgaaagagac
     4081 ggaaaactat atttatttta tacaggctgt caagaaattg atggtattag ccatcagcat
     4141 caatgtttgg cggtctctga agatggtatt cattttagca aatacagtgg taatcctgtg
     4201 gtagcagaaa aacagctagg tgaaaatggg ctaatacaag aatttcgtga tcctaaagta
     4261 attgttcaca acgatggtta ctatatggta gtagctacta aatctacaca gtctactggt
     4321 agaatagtga tgtttcaatc gtcagatttg tatacttggc acttttactc tgtgctgcta
     4381 gaagggactt cagagcaagg aataatgtgg gaatgtcctg atttattcca tttagacgga
     4441 cgagatgtct taatcatgtc tccgatacaa atcaagaaga acggactgga atatcataat
     4501 atcagttcta ccatggcttg tattggtgag gtaaactggg aaacaggtca gatgatagtt
     4561 gataatttcc atgaaatgga ttttggaatg gatttttatg caccacaaac aacaagagat
     4621 cctaatggtg gacgggtgat gattgcttgg atgcagatgt gggatcgtac attgcctaca
     4681 catgagttag gccatctttg ggcaggctct atgagtttgc cccgagagtt aagagtgaaa
     4741 aaaaatcgat tggtacaaaa acccatctca actgtatata gtcatttagt ttatcaacaa
     4801 ggcagtgaaa atattgcagt gactgacaca tcagtcgtat ttcgaaacgc gctgtctgat
     4861 tcttcatatt tacatctagt cgcagatttg gagaaagctg aagtttttac aattgaatta
     4921 ctgcggtcta agacggattg tctcaaacta gcctatgaca ctgctacaca ggtttttgaa
     4981 ttggatcgtc agaaactagg aagagagctt acaggggcgg aagaagaccc tctgaatgta
     5041 cgaaaagttg aagtaccttt gattgataat cagctcgttc ttgagatttt tagagatacc
     5101 tcatcaattg aagtctttat taatgggtta gaagttatga cggctacttt ctatgagctg
     5161 gaaaaagctc gagatatctt gtttcattcg atcggagaaa gtcgtattca agcttttgaa
     5221 acagcgagtg tgaaataaat gtttgattgc ttaggcgatg aatcaattat ttaataggtt
     5281 ttgaaggata atgtttttag aaaagaacag atagctagat ttcccatgga tttgctatta
     5341 gtattttgaa aaaattagta atttatgaca aaattcatta tactaaactt gtgcagaaat
     5401 gtgagggatc aactatgaaa gcaacaatga aagatgtcgc aaaattagca ggtgttggag
     5461 taggaactgt ttcccgggta ttgaataacg gttctgttaa agcttctact agagaaaaag
     5521 ttgaagcagc aatgacagag ttaaactatc aaccagattt ttatgcccgg gggttaaaga
     5581 tgaagcatac gaacacggtt gctttgattc tacccacggt atggcatcca ttttttggcg
     5641 aatttgccta ccatgtagaa acagcattag aaagacaaaa ctacaagctt tatttgtgta
     5701 actccagtgg taatagtgaa aaagaacaag aatatatcca gatggtcagc caaaataaag
     5761 tagacggcat cattggaatt acttattcag atatcgatga atacatttcg agtaatctgc
     5821 cttttgttag tattgaccgt tatttcactg aagaggtgat ttatgtgacc gctgacaatg
     5881 cggccggtgg acgtttggct gcaaatgaac tttatcgccg aggatgtcag aatgtagcat
     5941 atatcggtgg gtaccaagaa acaccgaatg aaacgaagag tcgtcgaaaa tattttgaaa
     6001 tcgagtgccg tcaactgggg atttcctata aagcgttaga tatgttagaa cctttagacc
     6061 atctctcaga acgtgtaaca aattttttgt tagagaatcc cgatatcgat gggattttta
     6121 cgatcaacga tccaatggca ttagctgtca ttgatatgct tgaaaaaata gggaaaaacg
     6181 taccagagga tgtccaagtt atcggatttg atggtcaaaa gatgacagaa ggagatcctt
     6241 atctagtctc aacgattgcc caacctacaa agcaaatggc ggaagtggca gtggcaaaat
     6301 taattgctgt cattaatgga gagacaatag aaaaacgtac cgtattgccg attgaatttg
     6361 gcgaaggtga cacaacgaaa cctttagtga gaaacgattt tgcttagtgc aaatacgttt
     6421 ctttttttga aaatgagtga atgaaaggtg tagataaaat gttatatggt ggaatagaag
     6481 ccggtggaac aaagtttgtc tgtgggattg gtaatgaaca attacaagtg atagaacgga
     6541 aaagttttcc taccacaaca ccagaagaaa cgatggagca agtgtttgat ttctttgata
     6601 agtacaaagg aaatatcgca tcaattggtg taggagcatt tgggcctatt gatattcaaa
     6661 tcgaatcaag aacttacgga tatatcacca acacaccgaa gttagcttgg caaaattatc
     6721 attttgtagg agctttggaa aacgccttgt cggttcccat ctattggact accgatgtaa
     6781 atgctgcttg ttatggagaa tatatggcag ggtatggaaa aggtaaaaat agtgttctgt
     6841 attacaccgt tggtacaggt gttggtggtg gtgctgtagt ggatgggaaa atagtcgcag
     6901 gttttagtca tcctgaaatg ggacatatgc tcttacaacg tcataatgaa gatcaatttg
     6961 aaggaatctg tccttttcat catgattgtt tggaagggtt agcatcagga cctgcgattg
     7021 aaaaacgctt tggaagaaaa gcccagacta tctcatttga tcatccttat tggacgattg
     7081 aggcagatta tcttgctcag tgtgtgtata acacaacgtt actatatgca ccggaagtaa
     7141 taattttagg cgggggtgta atgaaacagc cgcaactact cgagttgatt cgccaatcgt
     7201 ttaaagaaaa aatgggttcc tacgtggata cgcctactct cgaagaatat ctactaactc
     7261 caaggcttgc tgacaatgcg ggcttgattg gctgcctagc tttagcaata aagaaactca
     7321 atcattagaa tagtatttat tagaactatt cataacatac cggaagttta aaagggtatt
     7381 ctgaaactta aatagtctca attcattctt ctttcttgag aatctgagta gagactttta
     7441 ttctgtttca aattaatata attacgaaat gatattgagt acaagaaagt tctttttacc
     7501 agatctttta aatcgaaaaa tctgatatat atatttatag tacaaaaaat ctgattagat
     7561 aaaaagctaa ttcaatagga aaataaaggt agtgaataaa agcaaagccc ttgttagtag
     7621 ttaatctaac aagggctttg gtagggaaaa tggtaggaaa ccatcttatt ttttacgaaa
     7681 atcaggaact ttagtaattg aataatgttg atttataggt attcctcatc catattatgt
     7741 tggctcttta ttcccactcc actgttgctg gtggtttaga agtgatgtca tacacgatac
     7801 ggttgacgtg ggctacttcg tttacgatac gtacagagat tttttgcaag acttcccatg
     7861 ggatacgggc aaagtcagcg gtcattccgt cgattgaagt tacagcacgg ataccgactg
     7921 tgtagtcgta agtacgtccg tcacccataa caccgacaga gcggatccct ggaagaacag
     7981 tgaaatattg ccagatatca cggtcaagtc cagcagctgc gatttcttca cgcaagatag
     8041 catcagattc gcgtacgatt tctagcttgt cttcagtgat ttcgccaaga acacgaatac
     8101 caagaccagg accagggaat ggctgacgcc aaacgattga atcaggcatg cctaactgag
     8161 tccctaattc acggacttcg tctttgaata atgtgtttaa tggttcgatc aattcaaatt
     8221 gcatatcttc tggcagtcca cctacattat ggtgagattt gatcgtctgt gctgtttctg
     8281 taccagattc gataacgtca gtatacaacg ttccttgtgc taggaaagag acgccttctt
     8341 caccagcaag ttttgttgct tcgtcatcaa aaacatatac gaattcattc ccaatgattt
     8401 tacgtttttg ttctgggtca gatacacctt tcaacttgtc taagaagcga tctcttgcat
     8461 tgactttgat gatgttcaag ccgaattttc cgcctagact ttccattact tgttccgctt
     8521 cgcctttgcg tagcagaccg tggtcaacaa agatagatgt caattggtca ccgatcgctt
     8581 tttgtaaaag aaccccaacg acactagaat caacgccacc tgaaagacca agcaagactt
     8641 ttttgtcacc aacttgttca cggatttttt ctacttgcat atcgataaag ttatccattg
     8701 tccagttacc ttcacagtga caaacatcaa aggcaaagtg gcgcagcaat tcgattccgt
     8761 attcggaatg acgtacttct gcatggaatt ggataccata aaaattacgt gttgtgtctt
     8821 ggatagaagc aatcggacaa tctttgcttg ttccgactgt ttcaaagcct tcaggaactt
     8881 tagttaccaa gtcaccatgg ctcatccata ctgtttgttg ttttggtgtt tctttaaaca
     8941 agacagcttg atcgcttgtt acttctaaca aggctttccc atattcgcgg tttttagcag
     9001 gctctacgga accgccaagt ttgtacgtga tcaattgcat accatagcaa atccctaata
     9061 caggaatacc taaattgaag atctcaggat cgatcccaaa tgcattctca tcatatacac
     9121 tgtttggtcc gccagaaaga atgattccct ttggattcat cgcttttact tcttctgcag
     9181 taatgcggtg gctcaataat tcggaaaaga ctccaagatc acgaatacgt cttgtgatca
     9241 attggttata ctgactaccg tagtctaata cgataatctt ttcaacgttc gtcatatcgg
     9301 caacgtttgt caaatttatt acccctatcc ctttaaattt tatctcaaaa tagtcaaact
     9361 attttaaaat gctaatattt tcgcatgaaa atcgaatcga tgatatgatt ttcagtttta
     9421 tggagaatta tatcggctct tcctcgtgta ggcaagatat attcctgtaa atttttcaaa
     9481 ttcacatttt tccatacatc ttttgccatt ttaaaggctt cttcacgatc tccgatagca
     9541 aaacgataat aataattctc tggattttgg aaagccgtat ccagtaacgc accaaaacga
     9601 tccaaatacc atttttcgat cagcacggga tcggcatcga catagacaga aaaatcaaaa
     9661 aagtcgctga cgtagatttg ctgattcgca ggtaattgaa gcgtattgat cccctcaacg
     9721 atcaagatat caggctgctg gatcaattcg tacttgtctg gaatgatatc atacacatca
     9781 tgtgagtaaa caggtacttt tatctcttcc tgaccagatt ttacacggtt caagaaatcg
     9841 atcagcatct ccatatcata actttcagga aatcctttac ggtccatgat tccacgctct
     9901 tttaatatac gatttggata aagaaatccg tcagtcgtga tcagctgaac gtttcttcgt
     9961 ttgaaggtac gagacaaaat cgtctgcagc agtctggctg ttgtactttt tccaacggct
    10021 acacttccgg cgatcccgat aataaaaggc ggtacttcta cgtatttatg aagaaataaa
    10081 cctttactca agaacaaaga ttcaaattct ttcatataaa gataaatcag atgagcaaga
    10141 ggtacataga tatctcgaac atcctgcatg gaaatgcggt cattcaaact tttgatatta
    10201 tctagttcat cttgtgtcaa aggtgcttgc ccattacgat aaaattctcg ccattcttct
    10261 cttgccacac ggtagtagtt cattggatca ttcatattag cctcctgcgt tctcccatca
    10321 atggaactat tttaacacaa aataaacgca aaccccacca cttaagagag attgccgatc
    10381 tttttgtata atctttataa tagaaagaaa cggagtctta ttcttgactt gcgcacggat
    10441 acataaaagg atagactgtt gtcatgaaag aaatgagaag gatggaagac atgacaaatc
    10501 attactattc tgaacgccca gatacggcgc acgattttga acaatgggct tttgagttaa
    10561 aaggcagaac ctttcaattt gtaacagatt ctggtgtatt ttcacgtgaa acagtggatt
    10621 atggttcacg tgtcctgatt gatgctttcg attgggaaga gttacctgat gaaggaaaga
    10681 tcttagatgt gggatgcggg tatggaccga ttgggttagc tttggcattt gcgacacagc
    10741 ggtttgttga aatggtcgac attaatgaac gagcggtaag tctagcgcaa ggaaacgcaa
    10801 agcgtaacca aatcgaacat gttgatatcc atcaatcgaa tatctatgaa gcagttcatg
    10861 aagaaaccta tgcagctatc gtaagcaatc cgccgatccg tgctggaaaa aaagttgtac
    10921 atgaaatttt gacaggcgca tatcctcggt tgaagaaggg cggtacactg acaattgtga
    10981 tccagaagaa acaaggagcg ccgagtgcgc aaaagaaaat ggaagaaaca tttggtaatg
    11041 cagaaatcgt cacaaaagat aaaggatatt atatcatcaa aagtgtgaaa gaataactag
    11101 ctgaaaaata gtggagcgac gaaagagtgg aactgggcgt gcatgtacgg gtacgtatca
    11161 gttctttttt tgttgagaag tttttaaaaa caaagaacta ttttttcttc aaaaagaaat
    11221 agtaggaaca gagtcgttgt tttttacgat aaaataaata agatacagaa cttgtataga
    11281 aggctgccaa gtcggtagat aaaggaggtt tttatcaaaa aacatacttt ccttttttct
    11341 ctattctttt cttgggggtt ttggtacact gatagggaat ttagatagaa gggagccaat
    11401 aggatgaagc tgaccatcag agatatcgca gaaatggcag gcgtatcagt aacaactgtc
    11461 tcacagattt tgaataacaa aggaagcaga tttagtgata agacaagaaa aaaagtgttg
    11521 tcgatcgtga atgaacatca ttataagccc gattatttcg cttcaaatgt gatcaatcga
    11581 cattcaaaga ccatcggaat gatcgtccct gatgtcactg atttcttctt ctccaaactg
    11641 atcgaaggtg tagagagcta tttgaatcct ttgggctatg tgatcatgtt atgcaattct
    11701 cgtcatagcc aagaaaatga gatcaagtat ttacaagagc tttcccaccg ctcagtggac
    11761 ggaatcttgc tggccacacc aaatatttta cctgagaaat atgcattaga cagcggtttt
    11821 tatcaaaaaa tgccaatcat tttaatcgat cgcggattga atcgtcggga taacggacgg
    11881 cttatcgtaa aggaatatga gggcgcctat caagctgttt cgctgtttat tgaaaatggc
    11941 catacgaaga tcggcatgct gaaggaaacc actggatact atcaattaga agaacgtttc
    12001 aacggatatc gtcatgcgct gaaagatcat ggcctgcctt ttaatgggaa atttgttgaa
    12061 catggagagt tgactgtcca aggtggatat actgcatcaa aaaaattact caaacataaa
    12121 gatatcacgg ctattttttg cgggaatgat gccatggcga tcggatgcta tcaggcgatc
    12181 gatgaattgg gcaaaaaaat cccagaagat atctcagtca taggattcga tggattaaag
    12241 ctgtcagaat acatgattcc gcaattgacg acagtgcagc agccaagttt tgatattgga
    12301 ttttatgctg cacgtttcct gatcgatacg atcgaatttc ctcaaagaaa agttccgaac
    12361 aaagtgttcg aaacaaaatt aattattcgt gagagcgtaa aagtattgac aaagggctaa
    12421 ctcggcggta tattgcttat gtaagcattt acaaaggcta gttagtgctt tttttatttt
    12481 tagtaaacca gtttactaaa gtggtttacc tttcctaaaa ataatgtata ataccgaatg
    12541 aagacaagga ggatcaggca atgcgaatgg tggatctgat tgaaaaaaag agggatggaa
    12601 acgagctgtc gaaagaggaa atcgagtaca tcgtcacgaa ctatacgaat ggaaagattc
    12661 cagattatca agtcagtgca ttattgatgg caatttttta tcaagacatg acaaatgaag
    12721 aaatcacgaa cttaacattg gcgattgcta attctggtga tgtcatcgat ttgagttctc
    12781 ttgaaggaat aaaggtagat aagcattcga caggtggcgt tggtgacacg accacattga
    12841 ttttagcccc cctagtagct agtgtcggag tgacagtggc taaaatgtct ggacgcggtc
    12901 taggttacac aggtggtaca ctagacaagc tagaagcaat tcccggattt cagatcgagc
    12961 tttcggatga agaatttgta cgcattgtta atgaaagtaa agtagcggta attggtcaat
    13021 caggaaactt ggcaccggcg gacaaaaaat tgtatgcctt gcgtgacgtc acagcgaccg
    13081 ttgattctct tccgctgatt gccagttcga ttatgagtaa aaaaatcgcg gcaggagcag
    13141 atgcaatcgt tttggatgtg accactggag acggtgcttt catgaagaac atcgaagatg
    13201 caagacgtct ggcaaagacc atgacgagca ttgggaaact ggctaaccga gaaacagtgg
    13261 cggttatctc agatatgtca gaacctcttg gcgaagctat cggaaatagt ctagaagttg
    13321 tcgaggcaat cgaaacatta caaggaaatg gtccagaaga tctagtagaa atgtgctacg
    13381 ccttaggaag ccaaatggtt gttcttgctg gcaaagctaa gacaaccgat gaagcaagaa
    13441 cgttgcttca agaagctctg gaatcaggta aagcgttagc aaaattcaaa gaaatgatcc
    13501 aaaatcaagg gggagaccca gcgatcgtgg aacatccaga acgaattttg actgcacgct
    13561 acacaatgga gcttcctgct aaacaaagcg gagttgtgtc aaaaatcgtg gcaaatgaat
    13621 taggaattgc tgcaatgatg ctaggtgctg ggcgtaaaac caaagaagaa gatattgatc
    13681 acgctgttgg attgaaactg cataaaaaaa ttggtgacac tgtgacaaaa ggtgagtcac
    13741 tattgacgat ctatagtaac gacgaagaaa tcacttctgt cattgaatta ttatataaaa
    13801 atattgaaat tggtgaatcg gcaatgaagc caacgttgat tcatgacatc atcacagaat
    13861 aaaggaaaaa agcggacttt accgttttaa agaaaagggg aaaagaaatg gaactaaacc
    13921 gaatgatcga ccatactatt ttgaaagcag acgcatctaa agaagctgtg atgcaaatta
    13981 tcgaagaagc aaaaaaatac catttttatt ctgtctgcat caatccagca tgggttgcgc
    14041 tagcgaaaga acaattacaa ggtgagccag tagccgtatg tacagtaatc gggtttcctc
    14101 taggagcaaa cacacccgag acaaaagcat ttgaaacaac agatgccatc aataacggtg
    14161 cagatgaagt cgatatggtc atcaatatcg gtgctttgaa atcaaaggat gatcaacaag
    14221 tccaaaaaga tatcgaagca gttgttgaag cagcaaaaga tcgtgcgctt gtcaaagtta
    14281 ttattgaaac atcgttatta gatagagaag aaatcatccg agcttgcgaa atagcaaaag
    14341 ctgcaggcgc tgattttgtc aaaacatcaa ctggtttctc aacaggcgga gcaaaggtag
    14401 aggatgtccg tttgatgcgt gaaactgtag gaccagaaat gggtgtcaaa gcttccggcg
    14461 gtatccataa cgaagaagaa gcaatggcaa tgatcgaagc tggtgctaca agaatcggaa
    14521 caagcgcagg agtagcaatc gtatctggtt caactggtga aggctattaa ttttttcaaa
    14581 ggagaagctc atggcagaac tcaaacaaga atggatcaac acagctattg aagcgttaga
    14641 taaagcctat gttccttatt cccattttcc ggtaggcgct tgtctcgtaa cggaatcagg
    14701 caagatttat caaggcatca acattgagaa tgcatcattt ggattaacga attgtgccga
    14761 gcgcacagct tttttcaaag cagtatctga aggagaaaaa acatttacgc atctagtcgt
    14821 agcggggcac acccccgatc ctatctctcc ttgtggtgca tgtcgacaag taatggcaga
    14881 attctgttcc cctgatatgc cggttacgct agtaggagac aatggtgtga cgaaggcaac
    14941 gaccgttcgt gcactgctgc cttatgcatt tactgaaaaa gatctataac tcatgttcat
    15001 tcagtcagct gactgttgaa aaataaaaca attattttag gaggctttac agaaatgaaa
    15061 aaagcaaaat tatttggttt aggtgctgtc gcactagcag cagggttatt tttaggtgca
    15121 tgcggcaaca acggctctac tgattcatcc ggtggcaaaa gctcatcaga cacaacaact
    15181 gcagcattga tcacagacac aggtggtgta gacgaccgtt cattcaacca gtcagcttgg
    15241 gaaggactag aaaaatgggg gaaagatcaa ggactttcac gaggaaatga tggcttccaa
    15301 tatttccaat cttcaaatga atcagattac atcccaaata ttgaccaagc attgaatgct
    15361 ggattcaaaa cgatttttgg tattggttat aaactaaaac cagcgatcca agaacaagca
    15421 acaaacaata caggtacgaa ctttgttatt atcgatgatg tcatcgatgg attagataac
    15481 gttgtatctg caactttcaa agacaacgaa gcttcttatc ttgcaggtgt tgcagcagct
    15541 tatacgactg aaacaaacgt agtcggcttt atcggcggtg taaaaggtga agtaatcgat
    15601 cgttttgatg ctggattcaa agccggtgtt gatgcaggtg cgaaagaatt aggaaaagaa
    15661 atcaaagtat tgaatcaata tgctggtgac ttctctgctc cagataaagg acgttcaatt
    15721 gctcaaggta tgtatgcaca aaacgcagat atcatcttcc atgcttcagg cggtacaggt
    15781 aacggtgtat tccaagaagc aaaatcattg aacgaatcag gcgacaaaaa agtttgggtt
    15841 atcggtgttg accgcgacca atcagacgaa ggcgaataca cattaaacgg cgaaaagaaa
    15901 aacttcacat tgacatctac actgaaagca gttggaacag tagtagaaga tttggcacaa
    15961 aaatcagcgg acggtaaatt ccctggcggc gaacacactg tttatggatt gaaagaagac
    16021 ggtgttggtc taacagaagg acaactttct gacgaagcga aaaaagcagt tgatgaagca
    16081 aaagaaaaaa tcatttcagg tgacgtaaaa gttccagaaa ctcctgaaga aaactaaaac
    16141 catttctata gccttgttag acacatgttt gacaaggcta tggtttgatt agaacaagaa
    16201 ttgttcattt atccaagctg ccttgtgaca gtttggataa gggaacaact ccctgaagta
    16261 atgtttgttt caaaagaaga acgctcacta agcgtgaatc ttagtcaatc gttgccctag
    16321 atagaaatcg ttaggatgtg aaaaaaaggt ggttcaagaa aatattgtca tagaaatgca
    16381 gaatattacc aaagaatttg gaacattcaa ggcaaatgac aacatcaatt tacaagtaaa
    16441 ggcaggagaa attcatgcgt tgcttggaga aaacggtgct ggcaaatcta cattgatgaa
    16501 cgtgctttcc ggattattag agccgacatc agggaaaatt ttgatgcgtg ggaaagaagt
    16561 aaagatcaca agtccgacca aagccaacca attagggatt gggatggtcc atcagcactt
    16621 tatgcttgtt gatgccttta ctgtaacaga aaacatcgtg ttgggaagcg agcctagtcg
    16681 cgcagggatg cttgaccata aaaaagcacg aaaagagatc caaaaagttt ctgaacagta
    16741 tggattgtca gtcaacccag atgcttacgt tcgtgatatt tcagttggaa tggaacaacg
    16801 ggtagaaatt ttaaaaacac tttaccgagg agcagatgta ctgatttttg atgagccgac
    16861 agctgtattg acccctcaag aaattgatga attaatcgtg atcatgaagg aattagtcaa
    16921 agaaggcaag tcgatcattt tgattacgca taagttagat gaaatcaaag cagtagctga
    16981 ccgctgtaca gttatccgcc gtggaaaagg aatcggtaca gtcaacgtta aagacgtaac
    17041 ctcacaacaa ttagccgata tgatggttgg aagaacggtt tcattcaaaa cgatgaaaaa
    17101 agaagcgaag cctcaagaag tcgttttgtc tattgaaaat ctagtggtga aagaaaatcg
    17161 tggattagaa gccgtgaaaa acttgaactt agaggttcgt gctggcgaag tacttggtat
    17221 cgctggaatc gatggaaacg ggcagtcgga gttgatccaa gctttgactg gtttgcgaaa
    17281 ggcagaaagc ggacatatca agctaaaagg ggaagacatc actaataaaa aaccccgaaa
    17341 aatcactgaa catggtgtag gacacgtgcc agaagaccgt cataaatacg ggttagtcct
    17401 agatatgaca ttatctgaaa atatcgcact acaaacctat catcaaaaac cttacagtaa
    17461 aaacggcatg ctgaattatt cagtaatgaa tgaacatgcc agagaattga tcgaagaata
    17521 tgatgttcga acaacgaatg aacttgttcc cgcaaaagct ttatcaggcg gaaatcagca
    17581 aaaagcaatt atcgctcgtg aagtcgaccg agatcctgat ctgttgattg ttgcaaatcc
    17641 aactcgtgga ctggatgtag gtgcgatcga atttattcat aaacgtctga tcgaacaaag
    17701 ggacaaatac aaagcagtgt tactgatcag tttcgaatta gaagaaattt taaatgtttc
    17761 ggatcgtatt gctgttatcc atgaaggaga aattgtcggt atcgttgatc cgaaagaaac
    17821 atctgaaaat gaattaggtc tattgatggc gggctatacc ttagaagaag cacgaaaaga
    17881 gttagaaaag ggggctggcg cacatgaatg atcgcagcga aaaattccgt aatttattag
    17941 tgcctgtact ttcagttgtt ttaggattga tcttaggcgc gatattgatg gctgcttttg
    18001 gttttcagcc catccaaggt tattcttcta tgctagatgc agcccttggt tcgcaaagag
    18061 ggataggtga aacgttaagg caagcaacgc cgttgatttt tacagcatta ggtttttctg
    18121 tagctaactc agctgggttt ttcaatattg ggctttctgg acaagcactt tgcgggtgga
    18181 tcgtcagtgt ctgggtggca cttgcgtttc cagatctgcc aaaagttgtt ttgcttccgt
    18241 tatgcgtgat cttaggtgcc ttagctggtg cgatggcagc ggctgttcca ggtttacttc
    18301 gagcattttt tggaacaagt gaagtgatcg taacaatcat gatgaactat atcttattat
    18361 acgtaagcac atttgcttta catgaagtga tgcctgaaag ctatcgctca agtttggact
    18421 cttccaatat gatcagtcaa aatgcctcac tgcggttacc atggttaaca caagcttttg
    18481 gcggctctcg cgtaaactca ggattatttt tagcaatcat cgcgcttatt ttcgtttggc
    18541 tgctgatgaa gaaaacgact ttaggatttg aaatccgttc tgttggattg aatccttttg
    18601 cttctgagta tgcaggaatg agtagtaaac gtacgatcgt tttgtcgatg gtcatttctg
    18661 gcgctttggc agggcttggc ggtgtggttg aaggattagg aacgtatcaa aacttctttg
    18721 ttcaaacgac ttcgctatct attggatttg acggaatggc tgtttccttg cttggttctg
    18781 gatcagcaat cggtattctt ttatcagcga tcttgttcag tattttgaag atcggtggac
    18841 ttggcatgca gacaggagca ggtgttccgt ttgagatcgt caatgtatcg attgctttga
    18901 ttatcttctt tgtcgggatc aacttcctga ttcgtttcgt catggctaaa gtcttccaag
    18961 ggaaaaaaca agaagagatc gttgcgacaa tagaatcgaa accaacgaat caaaaccatg
    19021 aagggggcga actataatgt cgactatcga attaattgcc ttgatcctta cttctacact
    19081 ggtttattca acaccgctaa ttctaacgtc actaggcgga actttttctg aacgaagcgg
    19141 gatcgtaaac gtagggctag agggaatcat ggtcatgggt gcttttagtt ccatcgtttt
    19201 caacttgacg atgagtccat cacttggttc tatgacacca tggatcgcta ttttagtggg
    19261 tggcgtcgta ggtatcctat tttctctgat tcacgcagta gcaacagtca gtttgcgtgc
    19321 tgaccatatt atcagcggga cagtaattaa tctgatggca cctgctttag gtgttttctt
    19381 gatcaaagca tggtatggta aaggtcaaac tgataacatc acgcagaatc tggggtattt
    19441 ttctttccca ggattagcag atattccggt cattggacca attttcttta aaaatacatt
    19501 cttacctgct tattgtgcta ttttgattgc tgtcctagct tgggtgatca tcttcaagac
    19561 gaaattcggc ttgcgcctcc gttcagttgg tgaaaatcct caagctgccg acacattagg
    19621 tatcaatgta tatgctatgc gttattcagg cgtactgatt tctggcttgt taggcggtat
    19681 gggcggtgct gtttttgctc agtcgatttc cggtaacttt tcagctggta cgatcgttgg
    19741 acaagggttc atctccatgg ctgctatgat ctttggtaag tggaatcctt taggggcaat
    19801 gggtgcctca ttgttctttg gttttgctca aagcttgagt atcattggcg cacaattacc
    19861 agttatttct tcgattccgc cagtattgct gcaaattgct ccatatttgc tgacaatcat
    19921 tgtgctggtt gttttcttag ggaaagcttc tggtccaaaa gcaaacggga agaactatat
    19981 caaatcaaaa taatctttta gcgaataaaa agggtgggtt acgggaagtg tttttcgtct
    20041 cgcccttttt gttttaaagg caataaaaat aaaaagaaag aaggaataat catgtttaaa
    20101 cgcgtacatc tgatcgtcat ggattcagta ggaattggag aagcaccaga tgccgataag
    20161 ttcggtgatg ttggttctga tacattagga cacattgcga aagaagcagg attgaacgtt
    20221 ccacatttag agcagcttgg tttaggaacg atccgtcctc ttgaaggtgt ggaatcagtg
    20281 aaagatcatc aaggctatgc gacgaaatta gaagaagttt ctgtagggaa agatacgatg
    20341 acaggccact gggaaatcat gggattgaat atcaaaactc catttcgtgt gtttcctaat
    20401 ggattccctg atgatctgat caaacaaatc gaagaatttt ctggccgtaa agtcgtatgt
    20461 aacaaacctt atagcggaac agctgttatt gacgattacg gtgaacacca aatgaaaaca
    20521 ggcgatttaa ttgtatatac ttctgccgat cctgtccttc agatcgcggc acatgaagaa
    20581 atcattcctt tagaagaatt atatcggatt tgccaattcg ttcgtgatat aacaaaagat
    20641 gatccgtata tgattggccg aatcattgca cgtccgtacg tgggtgaacc aggagacttc
    20701 acacgaacaa gcaatcgcca tgattatgcg ttgaatccat tcggacacac agtccttgat
    20761 tctttgaaag aagcaggaaa ggacgtgatt gctgttggaa agatcaacga tattttcaac
    20821 ggacaaggaa tcactgaatc tgtccgcacg aaaagcaata tggatggcgt tgaccagctg
    20881 ctgaaagtta tgaaaaacga tttttcagga atcagcttta ccaatttagt agattttgat
    20941 gcattatacg gtcatcgtcg ggatgtagtc ggctatgcgc atgcgattga agaatttgac
    21001 ttacgaattc cagaattgct tgaagcgatg gaagaagacg acttgctaat gattacagcc
    21061 gatcacggaa acgatccgac gtttactggt accgatcata cgcgagaata tgtaccgtta
    21121 ttggtttaca gtaaaaaaat gaaagaacaa ggggcattgc ctcaaggata ttattcagat
    21181 atttcagcaa cgattgctga gaactttagt gtttcttcga ctgaaaatgg cgaaagcttc
    21241 ttgaaattat taaaataatc tttttaaata ggagaaagca aatgaccaag ttaagcgaga
    21301 tgttacaaga aacgacccag tttatccttg ataaaggagt aaaagaaatt gattttggtt
    21361 tgattctagg ttcaggatta ggtgaattag cagatgaaat cgaagatgct gtaatgattt
    21421 cttatgatga gatcccgttc tttcctgttt cgacagtagt tgggcatgca ggaaagctag
    21481 tttatgggtc tctggcaggc aagaaagtgt tagccatgca agggagattc cactactatg
    21541 aaggtcattc catgcaaaca gtgacctatc cagtacgtgt gatggccgga ctaggggccc
    21601 attcagtcat tgtgactaat gcatgtggtg gtgtcaacga atcatttgta ccaggagatt
    21661 tgatgctgat taccgatcat atcaatttta ctggtgaaaa tcctttgatc ggtccgaatg
    21721 aagatgcaat gggccctaga tttccagata tgagtcaagc ttatacgcct gactatcaag
    21781 caacagcaaa agcagcagca gaaaaactag gtttggatct taaagaaggc gtatatatgg
    21841 gctattctgg accaacttat gaaacgcctg cggagatccg aatgtctcgg acaataggag
    21901 ccgatgcagt agggatgtcg actgttccag aagtcatcgt agcagctcac agcggattaa
    21961 aagtgttagg aatctcttgt attaccaact tagcagcagg tatgcaagcg aatctgagcc
    22021 atgaagaagt tgtagaaaca actcagagag tcaaacaatc ttttaaagca ttggttaaag
    22081 aaacattgac attattgtaa taggaggaac aataaaatga gtgtgcatat tgaagcgaaa
    22141 gaaggcgaga tcgccgataa gattttactt ccgggagatc ctttgcgagc taaatatatt
    22201 gcggagactt ttttggaaga tccagtttgt tacaatcagg tacgcggtat gttaggcttt
    22261 accggcaaat ataaaggaga acgtatttct gttcaaggaa caggaatggg aatgccatca
    22321 gccaccatct atgcccatga attgatccag tcgtatggtg taaagaaatt gattcgagta
    22381 ggtacatgcg gtgcattgtc aaaagacgtc catgttcgtg atttagtact agcacaaggt
    22441 gcagcgacca gttcttcgat gatcgaaaaa aatttccaag cctttcattt tcctccaatc
    22501 agtgatttca atctattact gaaagcatat gagatagcta aagaaaaggg ctatacggtt
    22561 catgtaggaa atgttctttc ggaagattct ttctacaaag atgatttgac agagacgttc
    22621 caattagctg aacttggcgt tttaggagtg gaaatggaag cagcggctct ttattactta
    22681 ggcgcgaaat accatgtgca gacattaagt ttgatgaccg ttagcgacca tttgatcacc
    22741 ggtgaagaaa caacagctgc agaacgtcaa tctacgttca atgaaatgat cgaagtagga
    22801 ttggaaacag cgatagcaga ataagaagga aatgaaaaag ctgccctgca ttaagtaata
    22861 agaacatcca agcaaaaata gctcctcatg tttttgactt ggctggactt attattgcaa
    22921 atgtaagctt ttgaacaccg tttaccaaga ggttgtgaat ctgccgttta actctgagtc
    22981 acaaggaaca atttcaaaaa acagcttttc atgtttgatg aatattgtga cttgtgaccg
    23041 agaagttggc aggagaaaac cgtttatacg aaaaaaggga ctgtgacaaa tcgtgtcaca
    23101 gtcccttttt tatagtgttt tttataggta gtatttttta gcaactgtta agtcatcatt
    23161 taattcataa acaagtggtt gacctgttgg aatttcaaga tccatgatgt cttcgtcaga
    23221 gataccttcg atatgtttag ctagggcgcg taatgagtta ccatgtgcag ctactaaaac
    23281 agttttgttg tctagtaatg ctggagcaat ttcatcttgc cagaatggca aagcgcgttc
    23341 taatgtcact ttcaagtttt ctccaccagg aatatcgcgt ttgtctaaca ttgcgtaacg
    23401 acggtcattt gctgcagaac cttcgtcagt ttcttccatt aatggaggaa gagtatcgta
    23461 agaacgacgc cagatgtgca cttgttcgtc gccgtatttt tcagctgttt cttttttgtt
    23521 caaaccttgt aatttaccat agtgacgttc gtttaagcgc catgatttga tttgtggtac
    23581 ccaaagttga tctgaatatt ctaagatcaa gttacatgtt ttgattgcac gagttaaaac
    23641 agaagtgtaa gcaacatcaa attcgattcc agcttctttg attttgcgtc cgccttcttt
    23701 tgcttcttcg attccttctg gtgctagatt tacatcagcc caaccagtaa attggttcaa
    23761 tgcattccat tcactaagac cgtgacgaga aaaaactaat tttggcatgt ttagctctcc
    23821 ttttaagata ggtttatatt tctgaaattt tcagttctgt tttattgtac aattttttgg
    23881 aagagatttc catcgaaaaa ttagataaga ttctatgaga aaacaagtag agaatataaa
    23941 aaaacgaaca gtgcccacgc aactgttcgt ttttaggtat tagatgcata attgctcgat
    24001 ttctttttca ggtaaattga tatatttata acatgttcca actgaaacac cgcattttga
    24061 agaaataaat tggatcgttt cttttttttc ataatagaga cgtcggattt ttttgatcgt
    24121 ttttgcatcg atttttggtc ttccgccgat ttttcctttt ttacgagctt catctaatcc
    24181 aactaatgta cgttctttga tcaatgcgca ctccatgttt gctaggtttt ccattaattg
    24241 gaaatagatt tttcccattt cgcgcgtatc gattccttca tcaagactaa tcaagtgcag
    24301 cccgcgatct ttgaataatt gcgtgagttc tgttaattgc cgcgtagatt tgcctaatcg
    24361 atgaagttgg caaatgacta gtgcatcgcc ttgttgcatg tcagcgatca ctgactccaa
    24421 atgaacagca gtagaatcat tttgttccac ttcatatgtc tcatggaaaa tttcttgaca
    24481 accaaagtca gataattgat caacctgagt tgttaaatca acgtcattta tggttgtgcg
    24541 tgcataacca ataattttca tggtgattcc tttctagcag tgattttcta actggatata
    24601 acagtaaaaa tgtagcataa agtacgtcaa aaagatagtt ttttcgcaaa ttttccgctg
    24661 tcttttttta ttggatgatc aagcagtttt tctttattta cgacggattg cgtcaagaat
    24721 ctcaacgcct ttttctgagc cgacaactac tttagaaacc atgtttagga aaagcccatg
    24781 ttctactacc ccaacaagtt catctaaata ggtagctaag ctttctggct gttcgatgac
    24841 ttctagatgc agatcaatga tgtaatgacc accatctgtg gttaagactt ctccgttttc
    24901 gtctgtacgc aagacgggtt ggaatctttt ttcttcaaaa atatgaagta gctgttggga
    24961 gccataaggg atcacttcaa ctgggagtgg gaacttccca agtttatcga ctaatttaga
    25021 agaatcaacg atccaaatcg tttcttttga gtacgtagcg actattttct cgaaaagcaa
    25081 agcagcgccg ccgcctttga tcccttgaaa atcagcactg atttcatcag caccgtctat
    25141 tgtcaaatcg actacgggga cttcatcaat actctttaac ggtattccta atttttccgc
    25201 ttgttctttt gtcgcatttg aagtagtaac tcctgtgata cgtaaacctt cttcacgcat
    25261 acggcgacca atctcttcta ccatataata ggcggttgaa ccggtcccga gacctacgat
    25321 catcccgtcc tttacgaatt tcgcggcttc aatgccagct aattgtttta aattcatctt
    25381 tgcgtccacc tttttttagt ttgagcatcg tgctttcctc actattttag cgtgatttac
    25441 ggtaaaaaga taccattctt ttttattcca ataaagcttt ttcaaaaaaa attgcatctc
    25501 ttgttgacat gttttttcat tcgtgatatt ctaacaaagg tgctttatct acaggtttct
    25561 gtgaagaaac gtgctgactg tttatcaaag catatttgat aaggatgcag acattgcatt
    25621 tattttttat cacaaaaaaa gaaccacctg gatgtgtgga actagacgca aatcgtggga
    25681 aggaggaaac aaggaatgcc tacaattaac caattagtac gtaaacctcg taaatcaaag
    25741 gtggaaaaat ctaattcacc tgctttgaac aaaggatata acagctttaa gaaaactcaa
    25801 actaacgtaa actcacctca aaaacgtggg gtagcaactc gtgtgggtac aatgacacct
    25861 aaaaaaccga actcagcttt acgtaaatat gcacgtgttc gtttgtcaaa cttaattgaa
    25921 gttactgctt atatcccagg gattggtcat aacttacaag aacacagtgt tgtattatta
    25981 cgcggtggtc gtgtaaaaga cttaccaggg gtacgttacc atatcgttcg cggtgcttta
    26041 gatactgctg gtgttaacga ccgtaaacaa agccgttcta aatacggaac taaaaaacct
    26101 aaagcttaat tttaattatt aaacaaatga aacttgcttc tactatatag aagtaattac
    26161 tatgaacaat atttcggaag gaggagttac ggatgccacg taaaggtcct gttgcaaaac
    26221 gtgatgtttt accagatcct atttataact caaaattagt aactcgcttg atcaaccgtg
    26281 taatggttga cggaaaacgc gggattgcag ctaacattat ctataatgcc tttgacttga
    26341 tcaaagaatc tacaggtaac gatccattag aagtgtttga acaagcaatg aaaaacgttc
    26401 aacctgttct tgaagttaaa gcacgtcgtg ttgggggttc aaactaccaa gtaccagttg
    26461 aagttcgccc tgaacgccgc gttactctag gtcttcgttg ggtagtaaac tacgctcgct
    26521 tgcgcggtga acacacaatg gaagaacgct tagcaaaaga aatcatggat gctgcaaaca
    26581 acacaggtgc ttctgttaaa aaacgtgaag acactcataa aatggctgat gccaaccgtg
    26641 cgtttgctca ctatcgctgg taagattctc tctgtttggt cggcttttat aagtcgacct
    26701 cagatagtgt ccttattttt agcaactaga tagtaaagag aggagaaaca aggagaatga
    26761 ccagagaatt ttctttacaa aacactcgta atatcggtat catggcccac atcgatgctg
    26821 gtaaaacgac tgctactgaa cgtatcttgt actatactgg taaaatccat aaaattggtg
    26881 aaactcatga aggagcttca caaatggact ggatggaaca agaacaagaa cgtggtatta
    26941 cgatcacttc tgctgctact acagcacaat ggaagggtca ccgaatcaac atcatcgaca
    27001 caccaggaca cgtagacttc acagttgaag ttgaacgttc attgcgtgta cttgatggtg
    27061 ctgtaactgt tcttgatgca caatcaggtg ttgaacctca aactgaaaca gtttggcgtc
    27121 aagcaacaac ttatggtgta cctcgtatcg tatttgcaaa caaaatggac aagatcggtg
    27181 ctgacttctt atattcagta tcaactttac atgaccgttt gcaagcaaac gctcacccaa
    27241 tccaattacc aattggtgcg gaagacgact tcacaggaat tatcgacctt gtaacaatga
    27301 aggctgaaat gtatacaaac gatcttggaa cagagatcga agaaacagaa attcctgaag
    27361 aataccgcga attagctgaa gaatggcgcg aaaaattggt tgaagctgta gctgaaactg
    27421 atgaagaact tactttgaaa tacttagaag gtgaagaaat cactgaagct gagttaaaag
    27481 aaggtatccg ccgcgcaaca gttaacgtag aattctaccc agtactatgt ggttcagcat
    27541 ttaagaacaa aggggttcaa ttactattgg acgctgttct tgattacttg ccatcaccgt
    27601 tagatatccc agctatcaaa gggattgacc ctaaaactga tgaagaagta gaacgtcctg
    27661 cagacgacag cgctcctttc tcagctttag catttaaagt tatgactgac ccattcgtag
    27721 gtcgtttaac tttcttccgt gtgtactctg gtgttttaca atcaggttca tacgtacaaa
    27781 acgcaacaaa aggcaaacgt gaacgtgttg gtcgtatcct acagatgcat gccaactctc
    27841 gttcagaaat ctctgaagtt tacgctggag atatcgctgc tgctgttggt ttgaaagaca
    27901 caactactgg ggacacacta tgtgatgaaa agagtctagt tattcttgaa tccatggaat
    27961 tcccagaacc agttatccaa gtcgctattg aacctaaatc aaaagctgac caagataaaa
    28021 tgggtgtggc tttacaaaaa ctatctgaag aagatcctac tttccgtgct gaaacaaacg
    28081 tggaaacagg cgaaacaatc atcgctggta tgggtgaact tcacttagat atcatcgttg
    28141 accgtatgcg tcgtgaattc aaagttgaag ctaacgtagg tgcgcctcaa gtatcttacc
    28201 gtgaaacttt ccgtgctggc actcaagccg aaggtaagtt tgtacgtcag tctggtggta
    28261 aaggtcaata cggtcacgta tggattgaat ttacaccaaa tgaagaaggt gctggtttcg
    28321 aatttgaaaa tgcgatcgtc ggtggggttg ttcctcgtga atacattcca gctgttgaaa
    28381 ctggtttgaa agatgctatg gaaaatggtg tcttagctgg ctatccatta gtagatatca
    28441 aagctaaact ttacgatggt tcataccatg atgtcgattc aaatgaaact gccttccgtg
    28501 ttgctgcttc tatggcatta cgtgcagcag ctaagaaagc taatccagta atccttgaac
    28561 caatcatggc tgtagaagtt gttatccctg aagattattt aggtgatgtt atgggtcacg
    28621 taactgctcg tcgtggacgt gttgaaggta tggaagctcg cgctggcggc caacaagttg
    28681 ttcgtgcgat ggttccactt gctgaaatgt ttggttatgc tacaactctt cgttctgcta
    28741 ctcaaggtcg tggtacattt acaatgacat ttgaccacta cgaagatgta ccaaaatcag
    28801 tacaagaaga aatcattaag aaaaacggcg gaaaagctga ataatttctt gaaaccggat
    28861 agtcctgcaa agagattatc caaagagact atatttttaa tatagtttcg tgtaaaataa
    28921 ttatcgatga tatgggcatg tggtgtacct tgtgtacatc atatacctat cagacaaata
    28981 aaaaatatat tgtattttta ggaggacatt ttaaaatggc aaaagaaaaa tttgaccgtt
    29041 ctaaaccaca cgttaacatt ggtactatcg gacacgttga ccatggtaaa actacattaa
    29101 cagctgcaat cacaactgta ctatctaaga aaaacggcgg ccaagctatg gcttacgatc
    29161 aaatcgatgg tgctccagaa gaacgcgaac gcggaatcac aatctcaaca gctcacgttg
    29221 agtatgaaac agatacacgc cactacgctc acgttgactg cccaggacac gcggattacg
    29281 ttaaaaacat gatcactggt gctgctcaaa tggacggagc tatcttggta gtttctgctg
    29341 ctgacggccc aatgcctcaa actcgtgaac acatcctatt gtctcgtcaa gttggtgttc
    29401 cttacatcgt tgtattcttg aacaaagtag acatggttga tgacgaagaa ttactagaat
    29461 tagttgaaat ggaagttcgt gacctattaa cagaatacga attccctggt gacgatgttc
    29521 ctgtagttgc tggatcagct ttgaaagctc tagaaggcga cgcttcatac gaagaaaaaa
    29581 ttcttgaatt aatggctgca gttgacgaat acatcccaac tccagaacgt gacaacgaca
    29641 aaccattcat gatgccagtt gaagacgtgt tctcaattac tggacgtggt actgttgcta
    29701 caggtcgtgt tgaacgtgga caagttcgcg ttggtgacga agttgaagtt gttggtattg
    29761 ctgaagaaac ttcaaaaaca acagttactg gtgttgaaat gttccgtaaa ttgttagact
    29821 acgctgaagc tggagacaac attggtgctt tactacgtgg tgttgcacgt gaagacatcc
    29881 aacgtggaca agttttagct aaaccaggta caatcacacc tcatacaaaa ttctctgcag
    29941 aagtatacgt gttgacaaaa gaagaaggtg gacgtcatac tccattcttc actaactacc
    30001 gtccacaatt ctacttccgt acaactgacg taacaggtgt tgttgaatta ccagaaggaa
    30061 ctgaaatggt tatgcctggc gacaacgtaa caatggaagt tgaattaatc cacccaatcg
    30121 ctatcgaaaa cggtactaaa ttctcaatcc gtgaaggcgg acgtacagtt ggtgccggtg
    30181 tcgttacaga aatcaaagct taattcactt aagttttaaa gtaaagcaac atttattcgg
    30241 acgtaatcgc tcggaacgaa ggtcttagga tctttgttcc gagctttttt atttattgca
    30301 ggatcaggaa aaaagacagg agtattataa atagaagaaa cacgaaataa aagagtcagt
    30361 attttctaaa tgaaacttta tgaaaacaaa atgtttttta caagaaaaag ccttgctttg
    30421 tgagtttata tatattataa tgacaaaggt gcgtttactt tagtgaattg cacagacgta
    30481 atcagaatga tttcgtcggc taagaaacga gaggttgcga cacgcccgga cgctttgcca
    30541 cgaacgagcg tgacggaaat tttcgtggag ctatgtctac ttttcaaaat aggcgaagga
    30601 gggaacaaga tggcaaaaca aaaaattcgt atccgtttaa aagcgtatga acaccgtatt
    30661 ttagatcaat cagcggataa aatcgtggaa actgcaaaaa gaactggagc tagcgtgtct
    30721 ggtccaattc cattaccaac tgaacgcagt ctttacacaa tcatccgtgc gactcataaa
    30781 tacaaagatt cacgcgaaca attcgaaatg cgtacacaca aacgtcttat cgacattgtg
    30841 aacccaacac caaaaactgt tgatgctttg atgaagctag acttaccatc tggtgtaaac
    30901 atcgaaatca aattataaaa ctaaaacaaa aataatggag gtgtactcat gaccaaagga
    30961 atcttaggga aaaaagtggg aatgacacaa atcttcactg aatctggtga attaattcca
    31021 gtaactgttg tagaagctac tccaaacgta gtactacaag tgaaaacaat ggaaacggat
    31081 ggctacgaag ctattcaagt tggttaccaa gatatgcgtg aagttttatc aaacaaacct
    31141 gcgaaaggtc atgttgcaaa agcaaacacg gctcctaagc gcttcattcg tgaattcaaa
    31201 aatgttgagc taggagaata tgaagtagga aaagaaatta cagtagatgt tttccaagca
    31261 ggagacatta ttgatgtaac aggtactacg aaaggtaaag gattccaagg ggttatcaaa
    31321 cgccacggac aaagccgcgg accaatggct cacggttctc gttaccatcg tcgtcctggg
    31381 tcaatgggtc cagttgcacc taaccgtgta tttaaaaaca aacgtcttgc tggccgtatg
    31441 ggtggcaacc gcgtaactat ccaaaacttg gaagttgtac gtgtcgatgc agaaagaaat
    31501 gttatcttga tcaaaggtaa tgttcctgga gctaaaaaat ctttaattac tatcaaatca
    31561 gctgtgaaag ctaaataatt gtaggaagaa aggaggaact aaggaatgcc gaatgtagca
    31621 ttattcaaac aagatggaag ccaaaacggt gaaatcacac ttaacgaaga aatcttcgga
    31681 attgaaccta atgaaagtgt tgtttacgat gcaatcgtaa tgcaacgtgc gtcattaaga
    31741 caaggaacac acgcggttaa aaaccgtagc gctgttcgcg gcggcggacg taaaccatgg
    31801 cgtcaaaaag gaacaggtcg tgcacgtcaa ggatctatcc gctcaccaca atggcgtgga
    31861 ggtggcgtag tctttggacc aacaccacgt tcttacagct acaaacttcc taaaaaagtt
    31921 cgtcgtttag caatgaaatc tgtattgtca gaaaaagttg ctgaaaacaa cttggtagca
    31981 atcgaaggct tgaacttcga cgcaccaaaa acaaaagaat tcaaacaagt tcttgctaac
    32041 ttatctatcg actctaaagt attggttgtt ctagaaaaag gtaacgactt tgctgcttta
    32101 tcagcacgta acttaccaaa cgtttctgta gtaacttctg ataatgtgag tgtgctagac
    32161 gttgtatcaa atacaaaagt gttagcaaca caaactgctc ttactcaaat tgaggaggtg
    32221 cttgcataat gaacttacta gacgtaatca aacgcccagt gatcactgaa aaatcaatgc
    32281 ttgccatgga tgacaagaaa tacacttttg aagtggacac tcgcgcaaac aaaactttag
    32341 taaaacaagc ggtagaagct gcttttgacg ttaaagtcaa aaacgtgaac atcgtgaacg
    32401 tacgtgcgaa attcaaacgt atgggcaaat atgcaggata tacaaaaaaa cgtcgcaaag
    32461 cgatcgttac attgactgaa gattcaaaag aaatccaatt attcgaagct gctgaataag
    32521 cactaataga taactcatta aattaggagg gaaaaacgtg gcgattaaaa agtacaaacc
    32581 tacctcaaat ggtcgtcgta acatgacagg ttctgatttt gctgaaatca caacatcaac
    32641 acctgaaaaa tcattgttac aaccattgaa aaaccatgct ggacgtaaca acaacggccg
    32701 catcactgta cgtcatcaag gtggcggaca caaacgccaa tatcgcgtga tcgacttcaa
    32761 acgtaacaaa gataacgttg ttgcaactgt tcaaacgatc gaatatgatc caaaccgttc
    32821 agctaacatc gcgttagtac actatgaaga tggagtgaaa gcttacatct tagcacctaa
    32881 aggactagaa gtgggtatga aacttgtttc aggtccagaa gcagatatca aagtcggaaa
    32941 tgctcttcca ttagaaaaca ttccagttgg tactgttatc cacaacattg aaatgaaacc
    33001 tggtaaaggt ggacaattga tccgttctgc tggtacaagt gctcaagtac ttggtaaaga
    33061 aggcaaatac gtattgatcc gcttgaactc tggtgaagtt cgtatgatct tagctgcatg
    33121 ccgtgctaca atcggttctg taggtaacga acaacacgaa ttaatcaaca tcggtaaagc
    33181 tggacgctct cgttggatgc gtaaacgccc aactgtacgt ggtagcgtaa tgaaccctaa
    33241 tgatcaccca cacggtggtg gtgaaggtaa agctcctatc ggacgtaaag caccagtttc
    33301 tccttggggt caaccagctc ttggttacaa aactcgtaac aaaaaagcta aatcagacaa
    33361 acttattgtt cgtcgtcgca aaaataaata attactttga ccatgtgtcg cacattttga
    33421 gaggaggttc accatgggtc gtagtttaaa gaaaggacct tttgtcgatg agcatttaat
    33481 gaaaaaagtt gaagctcaag caggggttga aaagaaaaaa gttatcaaaa cttggtcacg
    33541 ccgttcaaca gttttcccaa gttttgttgg atataccatc gcagtttatg atggacgtaa
    33601 acatgtacca gtttacatcc aagaagacat ggtaggacac aaattaggtg aatttgcacc
    33661 tacaagaact tatcgtggac atgctgctga cgataagaaa acgaaacgct aatttgaggg
    33721 gaggataaat ccaaatggca gaacaaatca catcagctaa agcaactgca aaaacagttc
    33781 gcacttcacc tcgaaaagcc cgtttagtaa ttgaccttat cagaggtaaa agtgtcgcag
    33841 atgcaatttc aatcttgaaa ttcacaccaa acaaatctgc tggaatcatt gaaaaagtat
    33901 tgatgtcagc agttgcgaat gcagaaaata actttgactt agatgttgaa aacttggtag
    33961 taagcgaagc ttttgttaac gaaggaccta caatgaaacg tttccgtcct cgtgcaaaag
    34021 gatcagcttc tccaatcaac aaacgtacaa gtcatatcac ggtagtcgta tcagaaaaat
    34081 aaggagggat aatcagtggg tcaaaaagta catccaattg gaatgcgtgt aggcatcatc
    34141 cgcgactggg atgctaaatg gtatgctgaa aaagagtatg ctgagttctt acacgaagat
    34201 ttaagaatcc gtaaatttat ttcaactaaa cttgctgatg ctgctgtatc aacagttgaa
    34261 atcgaacgcg caacaggtcg cgtaaacatt tcaattcaca cagctaaacc aggtatggtt
    34321 attggtaaag gcggttctga agtcgaaagc ctaagaaaag aattaaacaa attaactggt
    34381 aaaagaatcc atatcaatat cgtggaaatc aaaaaaccag atctagatgc aaaattagta
    34441 ggtgaaggaa tcgcacgtca attagaaaac cgtgttgctt tccgtcgcgc tcaaaaacaa
    34501 gccatccaac gctcaatgcg tgctggcgct caagggatca aaactcaagt atctggtcgt
    34561 ctaaacggag cagatatcgc tcgttcagaa ggatactcag aaggaactgt tcctcttcat
    34621 actttacgtg cggacattga ttacgcatgg gaagaagcag atacaacata cggaaaacta
    34681 ggagttaaag tgtggatcta tcgtggagaa attcttccaa ctaaaaaaaa cactgagaaa
    34741 ggagggaaat aatcatgtta gtacctaaac gtgtaaaaca ccgtcgtgaa ttccgcggaa
    34801 aaatgcgcgg ggaagctaaa ggcggaaaag aagtagcatt cggtgaatac ggtttgcaag
    34861 ctgttgattc acattggatc acaaaccgcc aaatcgaagc tgctcgtatc gcaatgactc
    34921 gttacatgaa acgtggtggg aaagtatgga ttaaaatttt ccctcacaaa tcttatactg
    34981 ccaaagcaat tggggtacgt atgggttctg gtaaaggggc acctgaagga tgggttgcac
    35041 cagtaaaacg tggtaaaatc atgtttgaaa tcgcaggcgt tcctgaagaa gtagctcgtg
    35101 aagcgttacg tctagcttct cacaaattac caatgaaaac taagatcgta aaacgtgagg
    35161 aaatgggtgg tgaatcgaat gaaggttaaa gaaatcagag aattaaccac tgccgaaatg
    35221 cttgatcaag aaaaacaatt aaaagaagaa ttgtttaatc ttagattcca attagcaaca
    35281 ggtcaattag aaaacactgc acgtattaaa gaagtacgta aatcgattgc acgcatcaaa
    35341 actgtgttgc gtgaacaagc taagtaatta tggaaggagg ccattaagcg tatgactgaa
    35401 ggaagaaatc aacgcaaagt ttatcaaggt cgcgtggttt cagacaaaat ggataaaaca
    35461 attaccgttg ctgtcgaaac aaagaaaaac caccctatct atggcaaacg catgaactat
    35521 tctaagaaat acaaagtgca tgatgaaaac aacacagcaa aagttggcga catcgtgaga
    35581 attatggaaa ctcgtccatt atcagctaca aaacgtttcc gtttgttgga agtagtcgaa
    35641 gaagcagtta ttatctaata acacgagatt ttcctatata gattgaatgc aaaatctgaa
    35701 aggaggatac acaacgtgat ccaagcagaa agtcgtttaa aagtcgctga taactcaggc
    35761 gcacgtgaaa ttttgacgat caaagtcctt ggtggatctg gtcgtaaaac tgctaacatc
    35821 ggtgatgtga tcgttgctac ggttaaacaa gcaacgccag gtggggttgt caaaaaaggt
    35881 gacgtcgtta aagccgttat cgttcgaaca aaatcaggag ctcgtcgtac agacggttca
    35941 tatattaaat ttgatgaaaa tgctgcagtg attattcgtg atgataaaag ccctcgtgga
    36001 acacgtatct ttgggccagt tgcacgtgaa ctacgtgaaa acaacttcat gaagatcgtt
    36061 tcactagcac cagaagtttt ataatcttga gacacttatc aaaggaggtg cgaaacagtt
    36121 atgtttgtta aaaaaggcga taaagttaaa gttatcactg gaaaagacaa aaacaaagaa
    36181 ggcgttgtac tagcagcgtt cccgaaaaaa gataaagtta ttgtcgaagg tgtaaatgtc
    36241 gttaaaaaac accaaaaacc ttctcaagct gctcctcaag gtggaattgt tgagatggaa
    36301 gcgccaattc atgtttctaa cgtgatggtg gttgactcta ctggtgtagc tggccgagtt
    36361 ggctacaaag aagtagacgg caaaaaagtc cgtgtttcta aaaaaaccgg tgaagtttta
    36421 gataaataaa agtttaagga aggagggtct tactgaatga accgcctaaa agaaaaatat
    36481 cttaaagaag taactccatc attgatggaa aaatttaact atagctctgt tatgcaaaca
    36541 cctaaagttg aaaaaatcgt catcaacatg ggtgttggtg atgcggtatc aaacgctaaa
    36601 aacttagata aagcagttga agaattaact ttgatcactg gtcaaaaacc aatgattaca
    36661 aaagctaaaa aatcaatcgc tggattccgt ttacgtgaag gaatgccaat tggtgcaaaa
    36721 gttacattgc gcggagaaag aatgtacgaa ttcttagaca aattggtttc tgtttcacta
    36781 ccacgtgtac gtgacttcca tggtgtaagt aaaaaagcct ttgacggtcg tggaaactat
    36841 actttaggta ttaaagaaca attgatcttc ccagaagtag attatgattt agtagataaa
    36901 gtacgtggta tggacatcgt tattgtaaca acagcaaact ctgatgaaga atctcgtgag
    36961 ttgttgacac aattaggcat gccattccaa aaataattaa aggaggcgaa ctacgtggct
    37021 aaaaaatcaa tgattgctaa aaacaaacgt cctgcaaaat tttcaacaca agcttatact
    37081 cgttgcgaac gttgcggacg tccacattca gtttatcgta agttccatct ttgccgtatt
    37141 tgcttccgcg aacttgccta taaaggtcaa attcccggcg tgaagaaagc tagctggtaa
    37201 aaagtaaatt cgcataagaa ggaggtaaaa gatcaatggt catgacagat ccaattgcag
    37261 attttctaac tcgtatccgt aacgcaaaca tggttaaaca tgagtcttta gaagtacctg
    37321 catcaaaaat taaacgtgac atcgctgaaa tcttgaaacg tgaaggtttc gttcgtgatg
    37381 ttgaatacat cgaagatgac aaacaaggcg tgatccgtgt tttcctaaaa tttggtaaaa
    37441 acggcgaacg tgttatcaca aacttaaaac gtatctctaa accaggtttg cgtgcttatg
    37501 tcaaagctga cgaagtacca aaagtattga acggtttagg tatcgcaatc atttcgactt
    37561 ctgaaggtgt tgtcactgat aaagaagctc gtgaaaaaaa tatcggcggc gaagttatcg
    37621 cttacgtatg gtaatcaagt agaatctaca aggaggtgtc tttaagtgag ccgtatcgga
    37681 aataaagtcg ttgttatccc tgaaggagta acagttactc aagacggaaa caacgttaca
    37741 gttaaaggac caaaaggtga attgactcgt actttttcag cagatatcaa aatgaatatc
    37801 gaaggaaacg aagtaacttt cactcgtcca aacgacagta aagaaatgaa aacaattcat
    37861 ggaacaactc gtgcgaactt caacaacatg gttgtaggtg tcagcgaagg tttccaaaaa
    37921 gcgttagaac taatcggggt tgggtaccgt gcacaattac aaggatcaaa acttgtattg
    37981 aacgttggtt actcacatcc agtagaaatc acaccaccag ctggagtaac tgttgaagta
    38041 ccagctaaca cacaagtaat tgttaaaggt gcgaacaaag aagaagttgg cgaattagct
    38101 gcaaacattc gtggaactcg tcctccagaa ccttataaag gtaaaggtat tcgttatgtt
    38161 ggcgaatatg tacgccgtaa agaaggtaaa actggtaaat aatcgtttag aaaaattgca
    38221 tagaagccag cagcaggaaa aaggcatttg gctgaagttt accttcaaaa aaatacattt
    38281 ttacttgtgc cgcagcttct atttttcgaa acgtatttac tgcttgaaat agtagtacaa
    38341 taaaaataaa gaggtgacaa ttgtgattac aaaaccagat aagaacaaaa cacgtcaaaa
    38401 gagacatcgt cgtgtacgta acaatatctc tggtactgct gagcgcccac gcttgaacgt
    38461 ttttcgttct aacaaaaaca tctacgctca aattattgat gacgtagcgg gtgtaacgct
    38521 agcaagtgcc tctaccttgg ataaagaaat ttcaggtgga acaaaaacag aagcagcaca
    38581 agctgtcggt aaactagttg ctgaacgtgc agctgaaaaa ggcattaaag tagtagtctt
    38641 tgaccgtggt ggataccttt accatggccg tgtgcaagct ttagctgaag ctgctcgcga
    38701 aaatggacta gaattttaga agaaggagga ataccattca tggtttatat cgatccaaaa
    38761 catttggaat tagaagaccg cgttgttgca attaaccgtg taacaaaagt tgttaaaggt
    38821 ggacgtcgtc tacgttttgc tgctttagtt gttgtcggtg acaaaaacgg acacgtagga
    38881 tttggtacag gtaaagctca agaagttcct gaagcaatcc gtaaagcaat tgaagatgcg
    38941 aagaaaaact tagttgaagt acctatggtt ggttcaacaa tcccacacga agtaatcggt
    39001 gcttttagtg gtggtagaat cctaatgaaa cctgctgtag aaggttctgg ggttgctgct
    39061 ggtggaccag ttcgtgccgt attagaatta gctggggtag cagatatcac ttctaaatct
    39121 ttaggctcaa atacaccaat caacgttgtt cgcgcaacag ttgaaggttt gaaacaattg
    39181 aaacgtgcag aagaagttgc agaacttcgc ggcaaatcag ttgaagaatt aatcggataa
    39241 ggaggacaac aataatggct gaattaaaag ttactttaaa acgcagtatt atcggacgtc
    39301 ctcaaaacca acgcgataca gttaaagcgt taggtcttac aaagattaac agttcagtgg
    39361 ttaagccttc taatgaagca attaaaggca tgatcaatac tgtttctcac ctagtggacg
    39421 ttgaagaaat ttaatttaga aaaatcataa ctagaacatt aaggaggtgc caaacctatg
    39481 aaacttcatg aattaaaacc tgctgaaggt tcacgccaag tacgtaaccg tgttggtcgt
    39541 ggtacttcat ctggtaacgg aaaaactgcc ggacgcggac aaaaaggaca aaaagctcgt
    39601 tcaggcggcg gtgtgcgttt aggatttgaa gggggacaaa caccattgtt ccgtcgttta
    39661 ccaaaacgag gattcacaaa cgtgaaccgt aaagactatg cagtcattaa tcttgatgta
    39721 ttgaaccgct ttgaagacgg agctgaagta actcctgttg ctttagttga agctggaatc
    39781 gtgaaaaacg aaaaagctgg aatcaaagta ttagctaatg gggaactaaa caagaaatta
    39841 actgtgaaag cagcaaaatt ctctaaagca gctcaagaag caattgaagc ggctggtgga
    39901 tcaatcgagg tgatctaatg tttagactat taaaagatgc ttttaaagta aaagacatta
    39961 gatcgaaaat cctatttact gtatttgtgt tgtttgtatt tcgcctaggt gcacatatta
    40021 ctgtaccagg cgtaaatgct gcttcattgc aaaacctttc ttctttgcca tttttcaaca
    40081 tgttgaactt ggtaagtggt agtgcgatgc aaaacttttc cattttctca atgggggttt
    40141 caccatacat tactgcttct atcgtcattc agcttcttca aatggacatc gtgcctcgat
    40201 ttgttgaatg gtcgaaacaa ggggaagttg gtcgtaaaaa actgaatcaa gcgactcgat
    40261 atctgacatt agtattggca tttatccaat ctgtcggtat cactgctgga ttcaatcaat
    40321 acgctcaatt aggtttcgtg aataatccta acgtagcaac ttacgtaacg atcggtttga
    40381 ttctaacagc cggtacgatg tttgttactt ggctaggaga acagatcaca gaaaaaggta
    40441 tcggaaatgg cgtatcaatg atcatttttg caggtatcat ttctagattg cctgaaagta
    40501 tcagagaaat ttatgaagat tactttataa atattgatcc ttcagacata tggaaatctg
    40561 ctatttttgt ggccatttta ctagtagcgg tcttgttgat cgtgacattc gtaacatttt
    40621 tccaacaagc tgaacgcaag atcccgatcc aatatacgaa acgcgtagct ggcgcgccta
    40681 caagcagcta tctgccgttg aaagtaaatg cagctggagt tattccagtt atctttgcta
    40741 gttcgtttat tgctacgcca aatgctgttt tacaagcatt gagcggtaga ttcggttctg
    40801 aaaactggta caaagtgatg atggagatct ttaactataa tacagcacct ggtgctacga
    40861 tttatacgat tctgatcgtt gccttcactt tcttctacgc ctttgttcaa gtaaaccctg
    40921 agaaattagc ggaaaacttg caaaagcaag gaagttatat accaagcgta cgccctggta
    40981 aggggactga agagtatgtc tcaagattac tgatgcgttt aagtacagtt ggtgcagtct
    41041 tccttggttt agtggcattg cttccaatca ttgctcaaat ggtttgggat cttccacaat
    41101 caatcggatt aggcggtaca agcttactga tcgttatcgg tgtagcttta gaaactgcga
    41161 aacagttaga aggcttaatg ctgaaacgta aatatgttgg attcattgag taagaaagcg
    41221 tgttgaggtt tcccctcaac acacgttctc acacatttat taggagggaa aacaatgaac
    41281 ctcattttaa tggggctgcc aggcgcaggt aaaggaacac aagctgaaaa aatcattgat
    41341 gcttatggta ttccgcacat ctcaacagga gatatgttcc gcgcagcgat gcaaaacgaa
    41401 acagctcttg gcctggaagc aaagtcttat atggataaag gtgcattagt tcctgatgaa
    41461 gtaacaaatg gaatcgtgaa agagcgacta gctgaaccag atacagagaa aggcttctta
    41521 ttagatggct ttccacgtac gctagatcaa gcaaaagctc tggacgcaat gctaaaagat
    41581 ttgaataaaa aaattgatgc tgtcatcgat atccatgttg gtgaagaaat cttggtcgaa
    41641 cgtctagccg gccgatttat ctgcagcaat tgtggagcaa cttaccataa aattttcaac
    41701 cctacaaaag ttgaagacac atgtgatcgt tgcggtgggc atgaattcta tcaaagagaa
    41761 gatgataaac ctgagacggt taaaaatcgt ctggctatca acatcaaaaa tagtgaacca
    41821 atcttggctt attataaaga acaaggtttg ctaaacacta ttgatggtgc tcgtgaaatc
    41881 gatgctgtat ttgcagatgt acaaaaaatc attgaaaaat agcacgattg cccgtagccg
    41941 acttgcatta tgcagttact cttgtcaagt tcaataaatt atgatagaat atgtcagtcc
    42001 gacttctaga gaatcctcta gatgatccat tttgagaatt ttgaggtggg aactgcagtt
    42061 tccgccattt tatcgtgtga aaatgcgaaa caagtacaag gaggtactgt gcgtggcaaa
    42121 agaagatatg attgaagtcg aaggtacagt cgtcgaaact ttgccgaatg caatgtttaa
    42181 agttgaacta gaaaacggac accaagttct tgctactgtt tcaggtaaaa ttcgtatgca
    42241 ttacatccgt atcttacctg gtgacaaagt aacggttgaa ttgtctccat acgatcttaa
    42301 ccgtggccgt attacttatc gctttaaata attgtactcc gtaaaactac aggaggtatt
    42361 atcatgaaag taagaccatc agtaaaacca atgtgcgaac attgtaaagt aattcgtcgt
    42421 aaaggacgcg ttatggtgat ttgcccagca aatccaaaac ataaacaacg tcaaggataa
    42481 ttggaggtgc tataaatatg gctcgtattg caggagtaga tattcctcgt gataaacgtg
    42541 tagtagtttc acttacttat atctatggta ttggtaacac tactgctaaa aagatcttag
    42601 ctgatgctgg tgtatctgaa gatgttcgcg ttcgtgaatt aacgaatgaa caaacagatg
    42661 ctatccgtgc agaagtagat aaattaaaag ttgaagggga tcttcgtcgt gaagtgaact
    42721 tgaacatcaa acgtttgatg gaaatcggtt cttaccgtgg aatccgtcat cgccgtggat
    42781 tgcctgttcg tggacaaaac acgaaaaaca atgcacgtac tcgtaaaggc ccagctcgcg
    42841 ctatcgctgg caagaaaaaa taattcttaa gtgaaggagg ttaaaacttc atggcagcaa
    42901 aaaaagtgaa tcgtaaacgc cgtgtgaaga agaatataga agcaggtatt gcacatatcc
    42961 attctacatt caataacact atcatcatga tcactgatac tcacggaaat gcattagcgt
    43021 ggtcatcagc tggttcatta ggtttcaaag gaagcaaaaa atcaactcct tttgccgctc
    43081 aaatggcagc agaaactgct acaaaagcag caatggaaca cggattaaag actgttgaag
    43141 taacagttaa aggacctggt tctggacgtg aagcagcaat tcgttcatta caagctacag
    43201 gtttagaagt gactgcaatt cgtgacgtga ctccagttcc tcataatggt gcccgccctc
    43261 caaaacgccg tcgtgtttaa tgagtgccgc tcattttgag ttttatcagt aacgtcattg
    43321 aacaagacac ttttcgtttt gaaaggggta aagataagaa tgattgagtt cgaaaaacca
    43381 agaatcgcaa aaattgatga agaaaaagat tatggcaagt tcatcgttga acctcttgaa
    43441 agagggtatg ggactacttt agggaattcc ctacgtcgta ttttattatc ttctttgcca
    43501 ggtgctgcca tcactaatat tcaaatcgat ggtgtactgc atgaattctc taccatcaaa
    43561 ggtgttagag aagacgtcac tcaaatcatc ttgaacatta aaggcttagc gctgaaaatg
    43621 tatggccaag aagaaaaaac ccttgaaatc gatattaccg gacctgctac agtaactgct
    43681 ggcgatatta tcgttgatag tgatgtagaa attcttaaca aagatatgta catctgtagt
    43741 gtctctgaag gagctacgtt ccatgctcgc ttaacagtga aacctggacg tggttatgtt
    43801 caagcagatg aaaataaaaa agaagatatg ccaattggcg ttcttccagt cgattcaatc
    43861 tacacacctg ttcgtcgtgt gaactatcaa gttgagaaca cacgtgttgg acaccgtgaa
    43921 gattttgata aattgacgat ggagatttgg acagatggtt caattgaacc gttggaagca
    43981 atgagtttgt ctgcgaaaat catgactgaa catttggata tttttgtgaa cttaactgat
    44041 gaagcaaaaa atgctgaaat catggtcgaa aaagaagaaa cacaaaaaga aaaaatgttg
    44101 gaaatgacga tcgaagagtt agacttgtct gttcgctctt acaattgttt gaaacgtgca
    44161 ggaatcaata ctgtacaaga acttacaaat aaatctgagc ctgaaatgat caaagtccgt
    44221 aacttaggac gtaaatcatt agaagaagtt aaagcaaaat tgcacgactt aggtttaggt
    44281 ttacgtaaag aagattaaaa ccttttacga aggagggaaa ccaacgtgag ttatcgtaaa
    44341 ctaggacgca catctagcca acgtaaagcg atgttgcgtg atttaacaac tgatttatta
    44401 atcaacgaac gtattgtgac tactgaagct cgtgcgaaag aagttcgctc aactgcagaa
    44461 aaaatgatca ctttaggtaa acgcggagat ttgcatgcac gtcgtcaagc tgcggctttc
    44521 gtacgtaatg aagttgccag cgtgcgtgaa gaaaatgagg acatcgtcat tgaatcagct
    44581 ttacaaaaac tgttcaacga tattgcacct cgttacgcag aacgtcaagg cggatacaca
    44641 cgcattttga agacagaacc aagacgcggt gatggtgcac caatggttgt cttagaactt
    44701 gtctaaaatc acactaataa catcacttta tagatgattc ttttagagtg ttatgatgtt
    44761 ggaagatatt tttccgagtc tagctcagcg ctgctcctca agcgaatatc gcttggggag
    44821 taagcactca tcataaagtg tttttttata ccttcaatta cctgtcatga cagatatgtt
    44881 tgaaggcata aaaaaacatt ttaagcgaga aataattgaa aattttgtct ttttttgata
    44941 gtgttagtag gaactttagg atgaagaagg agtaatcatg gagccaatta ttgaattaga
    45001 aaagataaat tataaatacc agccagatga tcttcgcccc gcattgaaag atgtctcctt
    45061 tacaattgat aaaggtgagt ggatcgctat tattggacac aatggttcag gaaaatcgac
    45121 acttgccaaa acgatcaatg gattgcttct gccagaatca gggatcgtga aagtgggaaa
    45181 tcaaatattg gatgaagaaa atatttggac gatccgacag atggttggaa tggtttttca
    45241 aaatccagac aatcagtttg ttggttcgac agtagaagat gacgttgctt ttggtcttga
    45301 aaatcaaggg attcccagag aagaaatgct agtgcgtgtg aaagatgcac ttgagaaagt
    45361 tagaatggct gagtttgctt cacgcgagcc tgctcgttta tcaggagggc aaaaacaacg
    45421 tgttgccatt gccggcgtgg ttgccttacg acctgatatc attattttag atgaagcaac
    45481 cagtatgcta gatccagaag gtcgtgaaga agtgatctct acgatcaaga agatcaaaga
    45541 agaaagtcaa ttgaccgtta tatctatcac tcatgatatt gatgaagcag ctaatgccaa
    45601 tcggatacta gtgatgagac aaggcgaact tgtccgtgaa ggtacaccaa aagaaatttt
    45661 ttctgctggt cctgaactga tcgacttagg tctagatcta ccatttcctg aaaaactgaa
    45721 gagcgcactg aaagaacgtg gggttgatgt gcccagtgaa tatatgacag aagaaaggat
    45781 ggtggattgg ttatggacat ccgttttgaa caagtagact tcacttatca gccgaataca
    45841 ccatttgaac aaagagcctt atttgacatc aatatgacga tcaaagaaaa cagttatact
    45901 gcattagtag ggcataccgg aagcgggaaa tcaaccttac ttcagcattt gaatgcgctt
    45961 gttaagccaa caagcggtac agtccatatt ggagaacgag atattcagcc ggatacggat
    46021 aataagaatt taaagcccat ccgaaaaaaa gttggcatcg tctttcagtt tccagaagca
    46081 cagctattcg aagaaacggt agcaaaagac attgctttcg gtcctaaaaa ctttggtgtc
    46141 agcgaagaag aagcactagt cctagcaaaa gaaacattag aacaagttgg gctggatgaa
    46201 agctatttgg aacgttcgcc atttgaactt tcaggagggc aaatgcgtag agtagcaatc
    46261 gctggtgtgc ttgccatgag gccggaagta cttgtattgg atgaaccaac ggcaggactt
    46321 gatccccagg gcagaaaaga aatgatggag atgttttggc gtcttcataa ggaacagcag
    46381 atcactattg tattagttac gcatttaatg gatgatgtag ccaattttgc agattatgtc
    46441 tatgtgttgg aaaagggacg aatcgttaat agcggggagc cgcaagaggt ctttcaaaac
    46501 attgaatggc taaaagaaaa acaactaggc gtacctactg ccaccgagtt tgcagaagaa
    46561 ttaatggcta aagggatgaa ctttgctacc ttgcctttga cagctgaaga attagcagac
    46621 gctatcgtcc attatgctgg aggaaatgcc catgatgaat aaattgatat ttggtcgtta
    46681 tatgcctgga gattccttga tccatcgatt ggatccccga gctaaattat tagccagttt
    46741 ttattttatc ggaatcattt ttttagcaaa taattgggaa acgtaccttt tgttggcatt
    46801 tttcacatta ctttctgtct tcttgtcaaa agtagatttg aaatttttta tccgaggcat
    46861 tcgcccattg atctggctaa tcctcttcac tgtagctttg caggtcttgt ttacaagtgg
    46921 cggagaaact tattggagtt ggggtgtttt ccatatcact gaatttggtg tgatgaacgg
    46981 cctctttatt ttctgtcgat tcgtgttgat catttttatg tcgactctac tcacgcttac
    47041 tacaccgcct ttggaattat cggatgcgat tgagtacttg cttcgtccat tgaaagccgt
    47101 acggttccca gtccacgaaa tttcattgat gctgtcgatt gctcttcgtt ttgtgcctac
    47161 attgatggac gaaacagaaa agatcatgaa cgctcagcgc gctcgcggtg ttgattttgg
    47221 tgaaggaaac ctagttcaga aaatgaaagc agtcattcct ttgctgattc ctttatttgt
    47281 cagcagcttc aaccgtgcgg aagatcttgc gactgctatg gaagcaagag gctacaaagg
    47341 tggggaagga agaacgaaat atcgtgtatt gcattggcat aatagagaca cgatggttat
    47401 cattgctttt gtattattaa ctgtgctact gatcttttta agaggataaa cgttcagtgg
    47461 gccgctatct attcttttct tgctgatctg tttgcaagaa aggatgcaag cagtgtatga
    47521 caagaaatat tagggtctgt ggcagactaa ggtcacaggc ctctttcttt tatctgaaaa
    47581 gaaagaacga aaaaatttta tagaaaaatc attattccag ataacctttt aggaacaaag
    47641 gagagaaaaa catggtacgt tacaaagcaa tcattgcgta cgacggaaca aattttaatg
    47701 gattccaaaa acagccaaat gggcgtactg ttcaagaaga agtagaaaaa acattacaaa
    47761 aaatggcaaa tggaaaagaa atcacggtct ttggttcggg aaggacagat gccggagtac
    47821 atgcgatcgg gcaagtaatc cattttgatt atccagagga gcgtccgtta gaacgaatgc
    47881 gtttcgcgct agatacccag tcaccagagg atattgctgt aagacaagta gaaattgttt
    47941 cagaagactt ccacgcaaga tatttggtca aagaaaagac gtatcaattt cgagtggata
    48001 tagggaaacc acgcagtcct tttcgacgcc attatgcaag ttattttcct tacccacttg
    48061 atttggaaaa aattcagcgg gcgcttcctg atttattagg gactcacgat ttcacctctt
    48121 tttgtgcatc aggaagctcg atagaggata aaatacgtac gatctacgag gctaagatgg
    48181 aggtaaatga aacaaaagac gaattgttgt ttacatttag gggaaatggc ttcttgtata
    48241 agatgatacg gatcttagta ggaacgcttt tgaaaattgg caatggccga ttgcctgaag
    48301 atagtatccc agatattatt gcgaaaaaag atcgtaatgc agcaggacct acagcacacc
    48361 ctgaaggact ttatttgtat gaagtgaaat atgaataaga ggttatgata gaagtggtca
    48421 gcttcaagaa ataagaaggt atttacgaaa attacttctc aaaattttgc aaaatttcgg
    48481 cttattttcg aagaagctac ttctggaaca ccgtttaagg aagaggtcgt gaaatcagcg
    48541 ttttgacctg agtattagag aaacaaacgg gaaaatagct cctcatattt tgccgtttgt
    48601 tgagctaata ccgaaggtca gctgattgaa caccgtttaa gaaagaggtc gtgagcctgt
    48661 catttaactc tgagtcacaa agagcaattt caaaaaacag cttctcatct ttgatgaata
    48721 tcgtgacttg tggacgaggc gttgataagt gaatcatctt tttgttacct attttttctg
    48781 taaccaaaaa acgatggaac gttgttttga ttgcccttgg tgcggacagt ctgaacaggt
    48841 tccatcgttt ctttattttc tacgtcttct tctgtgtgtg tttcgtggtt gttcttctat
    48901 tgctggatca atcgtgccat aaaaggggcg attgcggtca aggtaccaga cataaagaat
    48961 aaacaatgca atacatagga caaaaaggat tgcacctatc gtaaatcctt caggcaagta
    49021 actgaaagtg acagtatgtt tacctgctgg aacttttatc atcaagaatg cgtctttgaa
    49081 attgctgacc ttgactggtt tcccatcaat tttagctttc catcctttgt cataaggaat
    49141 agttgtcacc aacatggaat cttcagcggt atttacagtc ccagaagctt ttctgccagt
    49201 ggcttccatt tcgacacctt tttctttgat ctgattcatt gcggcttgat aggctacaac
    49261 atctagacca acgactttag gctccatgaa acttatttct ttcgtcccat aaaaacttgc
    49321 agtaaatgtc actgttgtct cttgaggata atagccaaga tcatagtatt gaccagaaat
    49381 gttgatttgc gtttttcgcg atgtaccatt gacagtgatc gtcgcactag aactttctaa
    49441 ttgcgcaaaa ttggttggga acaaacttaa gtaagcttgt gtatttgcag gcacatggac
    49501 ttcccaagtg agctcttttg gctggttatc tgctgtttct gagtaagtga cgccagtcgt
    49561 tgtctttgtg atcgtcgtat tcaactgatt cttcaaagta ggtgtataga atgtaaaata
    49621 ctgttcattt agaccagaca atgtattgat caagtttgtc tgactggtca aattatctgt
    49681 cactggctgc tcaacatcat taacggacaa cggtgcagta aatgctagtg gcaatgcatt
    49741 gacattctga tacagtgtat agtcgccgct ttgaccagct tggcggaagc catatttact
    49801 gaacgtgctt tcgtttttag aaatgttata tttgattcct gtaaacccat ccatcagcaa
    49861 agtattattg ttataacgga tattcaaatt tgtccctctt gaacggaaac ctaatttatc
    49921 caggtaagag gaagaattac gatttcttat agaagaaaac aaactgatac cactgtaacc
    49981 atagttgatc gaatcgtttg cagatactgg atccagattt tccaagcggt aaaacgagtc
    50041 ggaatctttt tttgtttcat ttacaagagc tttcaagtca ggataaggat cagtatataa
    50101 actgcgggaa gcataattcc aatcatcaag aatgccatgg atcatactgt tcgtattgac
    50161 cagtgcttca gcagacatga tcaataataa caatatactc agatgattga atgaaagttt
    50221 tttatgctga taaaatccca cagcaaaagc ataaatccct aagaaaacca ctgtcaatac
    50281 aaaggaagtc atcgtcacat aggtgtagct tccttcaggt ttgatgcccc atgccaccgc
    50341 aaagagagca gctacgacca aaatgattcc taacaacagc ccacgatcgt cttgttctat
    50401 cttttcccat ccttttcctg caagcaggag cactaagaaa gagaacagat aggcataacg
    50461 gaataaaaac atattcggtg catgcatacc atgccagaac aaattcaatg gaacaagata
    50521 aaaacttgcg ataagtatgg cgaaaagact gccgtacaaa aatttatttt tcaacggtac
    50581 tttccgactg gcaaaataaa acagacagag gactaatggt agcagacctg cgtaaacgaa
    50641 cggaatcgat ccgtattttg tcgtgtcata aacaccaatc atatttttca tgattagatc
    50701 cagataagaa gtcgcttctg ttttcaacgt agtgatctga ctcagtgcct cgccatttga
    50761 ccgaagatcc agcactgctg gcaatacaat gatcattgaa gcgccgcctg ccagtaaaga
    50821 agtgatacca taaggaacaa tcgttttttt atattctgac cagtttgtca atagacgggc
    50881 gataaaatat aagaaagaaa atacgccaat cataaaaccc atataaaaac tggataaaaa
    50941 caagagtaaa taactgataa ataaaagttt cggccgttta agatccataa cacgatgaat
    51001 ccccaaaatc actaatggaa gataaaaaaa tgcatccagc cacatgatga tctccgaatg
    51061 tgcagtgata aatgagatca atgcgtaact gacgctaagt gcaacatgcc cccagcgagg
    51121 aatcttgaac gtacgtgcaa gaaaccaaaa agccaatccg gcacagccga ccttaagcaa
    51181 agtcagaaca tacagtgcat ctggcatcat ctggtttgga aaaaagaaca ccagtggcgt
    51241 gaagatcccg cctagatagt aagaaattaa agataaataa ttcaatccta gagaagcatt
    51301 ccatgtataa aaaatacttt gctttccaag aagaacattt cggaagctgg catgaaaatt
    51361 agaaaactgt gaaaaggcat cacttgccaa aatacttctg ctgcttccag gatagattcc
    51421 gatacttaga taaacaagtg ctaaaatcaa aaacggaatc aggaaactag ctgctgcgta
    51481 gcgtccgttt tctctaaaga atgactttaa attttttttc atatttattt tccaccaaat
    51541 ttctatgaag ttgtattcag tttatcatat ccttgttttt ctctgcttaa aaagagtcta
    51601 gttttcacaa aaaaataaaa tttagaataa ataacttcat aatttgttcg tataacaaaa
    51661 ataaacgcaa aaaaagggta ggaatcaaaa gtcccaccct taaaaatttt tattaaataa
    51721 aaaaccgttc actatctgaa cggtccaagt atcattcaat taaacgcgtt caacttttcc
    51781 tgatttcaaa gcacgagttg acacccaaac ttttttaggt tttccgtcga ccataacgcg
    51841 aactttttgt aagttaggtt taacagtacg ttttgttgag ttcatagcgt gtgaacggtt
    51901 gtttccactt tttgtcttgc gacctgtaaa gtaacaaact tttgccatgg taatttcctc
    51961 ctttgccttg atttggagca atagaataat tcagctcata ccagactaac atatcatata
    52021 gaatgggaat ttgcaagtac attttcatag atacaattga caattgagaa gaataattgt
    52081 aaattaaaca aggttatacc tgaaattttg caaggttttt gagtactttg ggtaactgcc
    52141 ttttatttca agttgcgcta tgataaaata tgatagaaga aaaatgggag ttttttaccc
    52201 gttaaggagg ctcattcatg gctgtaaaaa ttcaaacgtc agctggaacc attgaaatta
    52261 ccaatgatgt aattgcaaca gttgttggtg gcgccgcgac agatgtgttc ggtgttgtag
    52321 gaatggccag caaaaatcaa attaaagata atattactga gattttgcgt aaagaaaatt
    52381 actcaagagg agtagttgtc cgccaagaag ataatggtat tgctgtagat gtttatacga
    52441 ttatcagcta tggtacgaaa atctctgaag tttcccgcaa cgtccaagaa aaagtgaaat
    52501 acaatctaga ggcattatta ggcgtaactg ccaattcagt taatgtcttt gtacaaggtg
    52561 ttcgtgtact gccagactag gcaggagact ggctggtaac tcagcagtcg atctgaagga
    52621 ggattatcta ggtgagtgta acagaaataa gtgcaagcca gttccaggaa atggtccagg
    52681 caggtgcgaa tcgtctgcaa gtaaatgcag aatacgtcaa ttcattaaat gtattccctg
    52741 tgccagatgg cgatacggga acaaatatga atctatctat gactagtgga gctaaggcag
    52801 tagtagattc aacctcagaa aaagtaggag aattagcagc aatgctttca aaaggattgc
    52861 tgatgggtgc gcgcggaaat tccggcgtta ttttgtctca attgtttcgt ggattttcta
    52921 agcaaattcc agatgttgtc tcattaaatg cttctgatct tgctgcagcc ttcactcatg
    52981 gagttgaaac tgcgtataaa gcagtaatga aaccagtaga aggaacgatt ttgacagtag
    53041 ctagagaagc agcgaaagct ggagaaaaga aagcaaaaag tactgatgat gtcgttgaag
    53101 taatgacagc tgttgtaaaa ggagcaaagc gtgctttagc taaaactcca gacttgcttc
    53161 cagtcctaaa agaagtcggt gtcgtagaca gtggagggca aggactgctg tttgtttatg
    53221 agggcttttt aagtgcattg aatggagaat tccaagcgga tgaaacatat gaaccttctc
    53281 cagcagaaat ggatgatatg gtcaatgcag aacatcatcg cagtgtccaa ggtcaaatgg
    53341 cgacagaaga tatccatttt ggttactgta cagaaatcat ggtaaaaatc ggagaaggac
    53401 ctacagtcga tagtacattc gattacgaag aattccgtaa ctatttagat ggtataggag
    53461 attctttact tgtggtcaat gatgacgaga tcatcaaagt ccacgtgcat acagaaaacc
    53521 caggagaagt cttgaattat ggacaaaaat tcggttcatt agtcaaaatc aaagtcgaca
    53581 acatgcgctt gcagcatgag acgattttgg aacacgatga agaagtgaac gagtttgcga
    53641 aaaaagaaat accacgtatc ccgtatggta tcgtagcgat cgctgctggt aaaggcgtcc
    53701 aagagctgtt tgaaagttta ggcgctaatt atgttatcag tggtggacaa acaatgaatc
    53761 caagtacgga agacattgtt aaagcgattc aagaagtaaa cgcagataaa gtgattgtct
    53821 tgccaaacaa taagaacatc ttcatggcag ctgatcaagc agcagaagta gcagatatcc
    53881 cagtagcagt tgttccttca agaacgattt cccaaggatt gacagctatg ctttcattca
    53941 atgatcaagc ttctttagaa gaaaacaaag aaacgatgac agaaatgttg tctagcgttg
    54001 tgagcggtca agtcactcac gcgatcagag atacctcaat tgacggtgta gagatcaaag
    54061 aaggagactt tttgggcatg atcgatggaa aaatcctgat ctctaaccca gatatcacag
    54121 caacagcgtt aagtacacta gaacaaatga tcaatgagga cacagagatc gtaacgatct
    54181 tggcaggtga tgaaggaaca gttgaacaag caaatgaatt tgctgagaaa ctaacagaaa
    54241 aacatcctga tttggaaatc gagatccacc aaggagatca gcctgtctat ccttatctat
    54301 tttctgctga ataatttctg tgtcagaata tctttctaac acagtattca ttcataaaag
    54361 gccttcggtt agtgatcagg tatgatcgct gaccaagtcc tttttttgtt aaaggaggga
    54421 ggaaaaattt atgcgttctt tagatgatcc aatcactatg ctggcaggag tcggagaaaa
    54481 aagagcagct agtcttgcca gcttgggcat ccaaacgatt gaagacttac tgacttatta
    54541 tccttttcgc tacgaagata ttcaggaaag aaatctaaac gagatccagg atcaagaaaa
    54601 ggttactttg aaaggaatcg tagtctcgcc tcctgtgatg aatcgatttg gttataaaaa
    54661 aagccgattg caatttcgta tgatgcaaga acatgatgtg ttcaacgttt ctttttttaa
    54721 tcaaccttat ctaaaagaaa aagtcatttt atcggaggaa atcgccgtat acggtaaatg
    54781 ggatgcaaag cgtaaagcct tgaacgggat gaagatttta ggttctcaaa caatggatga
    54841 cttttcgccg atatatcacg tcaacaaatc aattcgtcaa acgacattag ttgatctaat
    54901 caaaaaaggg tttcaagaat acggcacatt gattgaagaa aatctgccaa acaaccttgt
    54961 ggagaaatat cgactgcttg accgagcttc tgctgtaaga agtatgcatt ttccaaaaga
    55021 ccacgaagaa aaccatcaag ccaaacgacg tgtcgttttt gaagagttct ttttatttca
    55081 aatgcgtctt caaggattga aaaaagcaga aaaagcagaa acgaatggtt tggagatcct
    55141 ttatgatgtc caaaaattga aacaatttac tcaaaagcta ccttttgaac tgacaaatgc
    55201 acaaaaacgt gtaacgaatg aaatctgtcg ggatcttcgc agtccgcagc atatgcagcg
    55261 acttttgcag ggagatgtag gtagcggaaa aacagtggtt gccgcgattg cgttatacgc
    55321 taccgttaca gcaggtttcc aaggtgcgtt gatggtgcct acagaaatct tggcacaaca
    55381 acatatggaa agcttgcaac agctgtttga tcccaatgaa gtaaccatcg cattgattac
    55441 ggggtcaacg aaagcaaaag aacgacgtga gctccttgaa aaactagagc aaggggaaat
    55501 taacgtcgtg atcggcacac atgcattgat ccaagatgga gtcgaatttc agaatttagg
    55561 tctagttatt acggatgagc agcaccgttt tggcgtgaac cagcggaaag tacttcgtga
    55621 aaaaggctgg cgaccagatg tcttatttat gacagcgact ccgattccta gaacattagc
    55681 tattacagca tatggtgaga tggatgtatc tatcattgat gaacttccag ctggacgtat
    55741 cccgatcgaa acacgctggg taagaacacc tcaattggac agtgtactgg attggacgaa
    55801 aaaagaactg gcaagaggac atcaaatgta tgtcatctgt ccgctgattg aagaatcaga
    55861 agcattagat gtaaaaaatg cagtggaaat ctatgaaaaa cttcgtgaac tatttgcacc
    55921 aaaatatgaa gttggcttgt tacatggtaa aatgaagaat caggaaaaag aagtaatcat
    55981 ggaagccttc aaagacaacc agatgcagat attagtttcg actactgtaa ttgaagtagg
    56041 ggtaaacgtg ccgaacgcga ccgtcatgct gatcatggat gcggatcgat ttggattggc
    56101 acagctgcac caacttcgtg gacgtgttgg acgcggaagt gatgcttcct actgtatctt
    56161 agttgctaat cctaaaaatg aactaggtgt ggaacggatg aaaatcatga cagagaccac
    56221 caatggtttt gtactaagcg aaaaagatct ggaattacgt ggtcctggtg aagtatttgg
    56281 cttccgtcaa tctggtctgc ctcaatttgc gattgctgac ttggtgacgg atggaaatgt
    56341 tttggaggta gccagagaag aggctgcagc cctttggcaa ataaaggatt ggcagatgct
    56401 tcctgagtat cagcctttag cgcaaagcct ccaaatagaa aatcaggata atcgttattt
    56461 cgattagcag actgtgctat actttatact gatgacggaa aaaggagtgg aaacaatgaa
    56521 aattgccgta gatgcaatgg gcggagatca cgcacctcaa gcaattgtag aaggcgtggc
    56581 tttagcgcag caagattttc cagaagtaga attcttactt tacggaaaag aagcagaaat
    56641 caagaaatac ttaacaaatg aaaaaaacat cacgattatc catacagacg aaaaaatcaa
    56701 tagtgatgat gagccagtaa aagcaattcg acgaaaaaag actgcatcaa tggtccttgc
    56761 tgcacaagca gtgaaaaacg gtgaagcaga tgctgtattt tcagcaggga atacaggcgc
    56821 gctactagca gcagggttgt tcatcgtagg acgtatcaaa aatatcgaac gccctggctt
    56881 gatgtctacc ttgccagtag tagggaaaga aggtgccggt ttcgatatgc tggatcttgg
    56941 cgctaacgca gaaaacaaac cagaacattt gttgcaatac ggtattttag gttcttttta
    57001 tgcacgtaaa gtccgaggta tcggacgtcc tcgtgtcgca ctcttaaaca acggaacaga
    57061 agcaacaaaa ggtagtgaag tgacgaaaaa agcctatgaa ctccttcaga atgaaacaag
    57121 cttgaatttt attggaaacg tagaagctcg tgagctactc aatggtgtgg ctgatgtagt
    57181 ggtcacagat ggatttacag gcaacgctgt tttgaaatct attgaaggaa cagcaatgaa
    57241 tatgatgaat ctattaaaat ctgctattct aaatgaaggg atcaaaggaa aaatgggcgc
    57301 tatgctttta aaagacggct tacgttcttt gaaagcagaa atggattatt ccaaacatgg
    57361 cggtgccgtt ttgtttggct tgaaagcacc agtcattaaa actcatggag caacaggccc
    57421 cgatgctgtt cgctatacga tccgccaaat ccacacaatg cttgagacag atgtagttgg
    57481 acaattagtc gaacaatttg aaaaaagcga agaatagtat ttggatatct gaagcgatcc
    57541 gttccattga aaaaaaggtg gacattcctg aaaaatgcca gtaaaattag ccatggtgta
    57601 ggtaattgac aagtgtatga ttcttggagg tgaaaaagtt gactcgcgaa gaagtattta
    57661 acaaagtagc gaaaattatt tcaaatcatt ttgagataga taccgataaa gtgacggatg
    57721 aattgaacat caaagatgat ttaaaagctg attcaattag tattatggaa ttcgttttag
    57781 agcttgaaga tgaattcggt acggaaattt cagatgaaga cgctgaacaa attgaaacag
    57841 tcggtggcgc agtagattat atcagcagcc atcttaaata atttcaaata aaagcaaaga
    57901 aggataaaaa gctgcatgca gctttttatc cttctttttt taatcttttt gacacgtaaa
    57961 agtcattttt ctgataattc tggcgataaa cagaaattat gttgcaaagt aaagcggttt
    58021 tctatataat tattaataat tcattacaca aagataagaa taacctttgt tataaaacga
    58081 tttattctgt gtaatgtcct tttttattaa gaaaaacttc catttaggaa gcagagaggg
    58141 gtattcgatt gtccgataac caattattag aagttcagaa cctacataca ggattccgac
    58201 tcaaagatga ctattacgat gcagtagatg acgtttcatt tactttagac aaaaacgaga
    58261 ttttagcaat cgttggggaa tctgggtgcg ggaaaagtac cttggctact acgattatgg
    58321 gattacttga tcctaataac acaaaaatta caggtgagat tttatataac gatctgaatt
    58381 taatcgacct gaacgaaacg ctctataata aaatccgcgg aaatgatatc ggaatgattt
    58441 tccaagatcc gttgtctgca ttaaatcctc tcatgagaat cgaagaccag atcaaagaag
    58501 gactttccta tcacacaaag atgaatgcag aacagcgtca agctcgtgca ctagaattgc
    58561 tagaacaagt agggattcct aatcctgcga gagttggacg ccaatatccc catgaactat
    58621 caggcggtat gagacaacgt gtcatcatcg caatcgcaat cgcctgcaaa cctccaatcg
    58681 tcattgcaga tgaaccgacg acagccctag atgtaaccat ccaagcacag attcttgatt
    58741 tactgaaaga ccttcaggaa gaaacaaaaa cagggatcat cttgatcact catgacttag
    58801 gagtcgtagc agaaatggct gatcgagtgg cagtgatgta tggcggacag ttcgttgaag
    58861 tagcaggtgt aaaagaactg tttgatcatc ctaagcatcc ttatacacgc tctttactga
    58921 actctatacc acaagaagga aatcatgatg cagaactcca tgtgatcgaa ggtgtcgttc
    58981 cttctctgaa aaacatgcct cgtaaaggtt gtcggtttgc accacgtatt ccatggatac
    59041 cagcaagcga tcatgaagaa gagcctatcc ttcatgaagt cgcaccgaac cacttggtac
    59101 gttgctcctg ctataagcat ttccacttta gagacgaaaa aggagaggtg tagatgacag
    59161 aattaatcac aatcaaagac ctgaaagttc attaccctat tcgcagtgga ttcttcaatc
    59221 gagtaacgga tcatgtatat gctgtagatg gtgtggattt tattatcgaa aaaggaaaga
    59281 catatggttt agtgggagag tctggctctg gaaaatctac tacgggaaaa gccgttgtgg
    59341 gattagaaaa ggtaacagcc ggacaaatca tgtatgaagg aaaagatgta acgaagcgaa
    59401 gcaaccgcaa aaaaatgggt tataacaagg atgtgcagat gatcttccaa gattcaatgt
    59461 ccagtttgaa tcctaaaaag cgagtgttgg atatcattgc tgaacccatc cgaaattttg
    59521 aacggttaag cgatcaagaa gaaaagaaaa aggtaaaagg actgctagat atcgttggga
    59581 tgccggaaga tgcgctatat aaatatcctc atgaattttc aggcggacaa cgtcaacgtc
    59641 taggagtagc ccgtgctgtt gctaccaatc caaaactgat cgtagccgat gaacctgtat
    59701 ctgctctgga tctatctgtt caagcgcaag tattgaattt catgaaacga atccagcaag
    59761 aatacggcct cagctattta ttcatttcac atgatttagg tgtagtaaaa cacatgtgcg
    59821 acaatatcgc aattatgtac aaaggtcgat ttgtagaaat cggtacaaga gaagacattt
    59881 ataatgaccc gcgacatatc tatacgaaac gtctgctttc agctattccg caaatcgatg
    59941 tagaaaatag ggaaagccac aaagaaaatc gacgaagagt agaacaagag tatatccaaa
    60001 accaaaaaga atactatgat gcgactggtc gagtctacga tttgcgtacg atcacaacta
    60061 cacacaaagt ggcgttgaaa gatggaggtg caagctaatg tggaaaacga ttctgcgacg
    60121 tgttttattg atgatcccgc aagttattat tttaagtgtt ctgatcttct tattagccca
    60181 agcgatgccg ggggatccat ttaccggttt gatcaatcca aatcaggatc cggcagtgat
    60241 cgaacagatg cgacaagcag ctggcttgaa tgacccatgg tatgtccagt acttccgttg
    60301 gatcggaaat gctttgcaag gcgattttgg tcaaagtttc ttatacaaac gctctgttgc
    60361 tgacttgatc ggcgaacgga tcatcaacac tttatatcta tcgatcttga cagttgtcct
    60421 tacttatttg attgctgtgc ctctggggat gcttgctggc cggtatcaaa attcaatctt
    60481 ggataagatt gtcgtaatct acaacttctt tagttttgct gttcctttat ttatcttcgg
    60541 cttgatcatg ctatttgttt ttggttatcg tttaggctgg ttcccaacaa gtggatcaca
    60601 aacactaggt ttgcaaggtg gatttatcac tcaatggata gaccgtttga aatatattct
    60661 gttaccatca attacccaag catttttggc gactgccgtc acaatccaat atctgcgaag
    60721 tgaagtaatc gattcgaaat cgctcgattt tgtcagaaca gcccgttcca aaggggtacc
    60781 gacgaataaa gtattcagtc gacatatttt ccgaaatgca gcgttgccga tggcttctca
    60841 aatggggtat gaaatcactt cgttgattgc tggttctgta gttatcgaaa agatttttgg
    60901 ttatccggga gtcggaaaac tatttattga ttccatcggc caacgggact ataccgtgat
    60961 cacatcctta gtattgattt taggaatcgc gacgctagtg gggacattac tttcagatat
    61021 catcatgagt atcgttgacc cgcgtattcg gattcaataa aaccaatcag aaaggagaat
    61081 ggatcgatga gtgaaaaaaa caatacagca gttcaagaaa gtattcctcc tatggggata
    61141 cgaatgattg ctcgagaatt taaaaaagat aaaattgcta ttttttcatt aggtttgcta
    61201 gtggttttat tattagtgat ctttatcggt gcgatgctga ccgatcaaga caaagtgatg
    61261 tatgttagta tcttcgacaa atacgctgca ccaggaacag tttcgacaca aggacaaaag
    61321 ttcattctgg gagcagatga aggcggacgt gatgtattag gacaattgat cataggtgct
    61381 agaaactctg ttacgatcgg atttgcgatc acgatcctta cttcgatcat cggggttgga
    61441 ttagggatca tctctggcta ctacggaggg atcattgaca acatcttgat gcggatcgtt
    61501 gattttatca tgattctacc gatcatgctg atcattatcg tcttcgtatc gattatttcc
    61561 agatacaatg tctggtcctt tatattcatc atgagtgcat tttattgggt agctaaagcc
    61621 cgattattcc gaagcaagac attatctgaa gcaagaagag attatgtcag tgcctcaaaa
    61681 acaatgggaa ccaatgattt taaaatcatg ttccgcgaaa tcatgccgaa tctaagttca
    61741 ttaatcatta ctaatttgac gatgaacttt gcggcaaaca tcggtatcga gacaactttg
    61801 actttcttag gtttcggctt gccggcaaat gtacctagtt taggtacatt gatcggttat
    61861 gcaagtaacg gagaagtctt agtgaatcgg caatggatct ggttacccgc atcaatcctt
    61921 attttagttt tgatgttgag tatcaattat gttggacaag cattcaagcg ttctgcagat
    61981 gcgcgccaac ggttaggcta gtatagaagc gaagggggaa ataggaatga agaaaacgat
    62041 ttttggattt gcagcgctga tgtctgctgt cgttcttgca gcatgcgggg gcggaggtac
    62101 agatacctct ggaaacaact ctggaggcaa cggcaataac aaagaggaat taacttttcc
    62161 aattgcaaca aaaaataatg atgatgcaat cgaagacggc gaattagaag ctgcggtcgc
    62221 aacggacagt cagtttaaag gattgttcca gtgggagttt taccaagatt catatgatgc
    62281 agcttttatg gctccttctc atgaaccgct atttgcaaac gatgacgatt tcagaatcac
    62341 agacgacgga gctgcaacgc ttgatctaga tgaagatacg aaaaaagcga caatcaaagt
    62401 aaaagacaac gtcaaatggt cagatggtga agatgtaacg gcggacgatg tgattttccc
    62461 ttacgaaatc attgggaata aagattacac tggtatccgt tatgacgaca ctttccgtaa
    62521 catcgtaggt atggaagaat atcatgatgg aagtgccgac acaatctccg gaatcaaaaa
    62581 aatagacgac aaaacagttg aaatcgaata taaagaagta aacccaagca tgcttcaatc
    62641 gggtggtggt atctggtcat atgcagcacc aaaacatacg ttagaaggtg tagcaatcaa
    62701 agatatggaa tcaagcgatc aagtacgtaa aaatccagtg acttttggcc cttattacat
    62761 gagtaatatc gttgcaggag aatcagtaga atacttgcca aacgagcact actggaatgg
    62821 tacgcctaaa ttgaaaaaga tcacgatgaa atctgtaccg atggcaagtg caactgaagc
    62881 attgaactct aaattatatg atatgatttt ccaaatgcca acagatactt acgataccta
    62941 taaagaaatt gctggctata caaacttagg ccgtgaacaa acttcttata cttatctagg
    63001 attcaagtta ggtaaatgga atgcagatac aaagagtgtg gaatacaatc cagattcaaa
    63061 aatggctgat aaatcattac gtcaagcaat gggctatgca ttagataata atgcagtagg
    63121 tgaacgtttc tatgcaggtt tgagaagcaa tgcgacttca ttgattcctc cagtattcca
    63181 gtcataccat gatgatgaga tgaaaggatt cacacaagat ctagacaaag cgaaaaaact
    63241 attagatgat gctggctaca aagatgtgga tgacgatggt ttaagagaag acaaagatgg
    63301 aaataaacta acaatcaact ttgcttcgat gtccggtggg gaaacagcgc aaccattggc
    63361 agattactat atccaacaat ggaaagaaat tggtttggat gtccaataca caacaggtcg
    63421 tctgatcgaa ttcaattctt tctatgataa attagaaaac gatgatcctg aaattgatgt
    63481 ttaccaagct gcttggaata caggaactga tccaaatcca agtggactat ggggaccaaa
    63541 tgctgcgttc aactacactc gttatgaatc agaagaaaat acaaaattga tcaacgatat
    63601 cagttcaagc aaagcatttg atgaagagta ccaaaaagaa gcatatcaaa aatggcaaca
    63661 atatgcattt gaagaagcat ttgcgattcc aacgttgttc cgtaatgaag tattgccagt
    63721 caacgaccgt gtcaaagact gggattggtc ttatgatgta ccaaacccat gggcaaatgt
    63781 ttctgttaca gcagattcac gttcataatc actaaaaatg accgaaaaag ggcgatgtcc
    63841 tttttcggtt ttttgatatt tttctgttta tttctctaat attcaggtca aggcgctgat
    63901 ttcatgactt ctccaataaa cggtgttcaa agtccagttt ctcggtatta agtcaaattt
    63961 atgcaaaaat atggaaagcc attttcacaa aattttttct tcatactcga aactaaacga
    64021 atttttcaca acttctcgtc aatcactagg attattaaag ctttacttac cttttgaccg
    64081 aaccatttca aatttgacaa aaaaccagta taataaggaa gtatcgaaaa aatatccata
    64141 aaaggaatgt ggaaaatgga caaccagctg acaaaagaac taaaagaaaa ctataacatc
    64201 gtttttcacg atctaaattt gcttgaacaa gcttttaccc attcatccta tgtgaatgag
    64261 catcgcaatc tgcaattatc ggataatgag cgacttgaat ttttaggaga tgccgtttta
    64321 gaactgatgg tttctcagta tttattccgt ttatatcctc atttgccaga aggaaaattg
    64381 acaaaaacac gagctgcgat cgttcgcgaa gacagcttgt cgaaatttgc caaagaatgt
    64441 cactttgatc aatatgtttt gttgggcaag ggagaagaga attcaggagg acgtacgcga
    64501 ccagcattgc tttgcgacct attcgaagct tttttaggcg cgttatatct tgaccaggga
    64561 ttcgacacga cccatgcatt catcgaaaaa gtgatttttc ctaaagtcaa agcaggtgct
    64621 ttttcacatg agatggatca taaaacaaaa ctacaagaag tcttgcaaaa atcaggtgat
    64681 gtatcaatcg aataccgatt gatcaatgaa gaaggtccag ctcatgaacg agtattttgg
    64741 atcgaagttt atgtagatga tcaattgatt ggcactggac aagggaaatc gaaaaaatta
    64801 gcagaacaag ctgcggcgga aaatgcattg gcagcactgt aaaagtaagt gagggaaagc
    64861 tttgtatttg aaacgaattg aaatagccgg ttttaaatca tttgcagaca agacagtcat
    64921 tgattttgaa aatagcgtca cagcagtggt tggaccaaat ggcagcggaa aaagcaatat
    64981 tacagaggct atccgctggg tattaggaga acaatctgct aaaagtctgc gaggcggaaa
    65041 aatgccggat atcatctttg ccggttccga tacccgtaag cagctgaata ttgcagaagt
    65101 cacagtgatt ttagacaata gtgatcatta tctgccattg gagtacaatg agatcagtgt
    65161 aacccgccgt tatcgacgga ctggggaaag cgagtttttc atcaataaac aaagctgcag
    65221 attaaaagat atccaagaat tgttcttaga ttctggatta ggaaaagaat cgttctcaat
    65281 catttctcaa ggaaaagtag aagcgatttt cagtagtaaa ccggaagacc gtcgtgggat
    65341 ctttgaagaa gcagctggtg tcttgaaata caagcagcgg aaaaagaaag cagaacagaa
    65401 gctttttgaa acagaagaca acctcagccg cgtccaagac atcatccacg aattggaaga
    65461 acagttaact cctttggccg cccagagtga ggcagcaaag gagtttttac ggctaaaaga
    65521 aacactgact caaaccgatg tctctttgat ggttgctgaa atcaagacag caaaaaaaga
    65581 ttgggacaac aaacaagcac agcttgcgaa attcaatcta gaattaggca aactatctga
    65641 gtcgatccaa gaacaagaat ccattttggc gaagcagcgt aaagaaaatg cgcaagccga
    65701 tcgtctgatc gaaaaaaatc agcaagtatt attagatctt tcggaaaaac tgaagcagac
    65761 agaaggacaa aaagatgttt tgcaggagcg gacaaagcat acacaaaaaa gctcgcaaga
    65821 atatcaaact tcattagcag aagcacaaaa aaaggttaaa cattttgaaa agcttcaaga
    65881 aagcttgatg aaagcagcag cagaaaaaga aacagagatc caaaaagctg aagcaaatct
    65941 cataaaaaca cagcaagaat tagaaaaata ccaaaaatca acgaaagaat tattggcaga
    66001 attaagagat caatatgttg atttgatgca agaacaagca gctgtaggaa acgaactgaa
    66061 gtatctagaa cgtcaatata tacaagaaac agcaaaaagc aagcagacgt tagccaagca
    66121 atcggaagta gaagcatcag tagaccgatt gatcttacaa aaagaagaat tgacacaaaa
    66181 gcaggcgcag ctaaaatcat ctttaactga aacaaaagag aagttagaaa tgatccaaca
    66241 aaacgggaaa aagttccaag aagcattagc aaaagaacag cctaaaatgt atcaattaat
    66301 gaatcaagtc cagcagctac gtgctcgtaa gaaaagcctt caagaaatcc aagaaaacta
    66361 ttttggtttt taccaagggg ttcgattggt cttgcagcat aaacagcagc tttcgggtat
    66421 tgtcggagca gtggctgaac tgatcgatgt tcctgcagat ttcacgttag ctatcgaagc
    66481 agctttaggt ggggcagccc agcatgtcat cgttgaaaat gaaaaagatg ctcggcaagc
    66541 catcacgtat ttgaaacagc aaagaggagg aagagcaaca ttccttccac tgacgacaat
    66601 caaaccaaga caattaccgg ctcatatatt gacacaagca gcagctgtag aagggtttat
    66661 agggattgcc agtgaacaag tcagttatcc cgatcaaatc caaactgtgg ttcaaaacct
    66721 gctaggaacg atccttttag ccaaagactt aactagtgcc aatgcaatcg cgcaaacgat
    66781 ccgctatcaa tatcgagtgg tttccttaga aggtgatgtg atgaatgccg gaggatccat
    66841 gacaggtgga gcgaataaac gaggaaacca aggcagtttg tttgtacaaa atcaagaatt
    66901 gaaacagttg actagtgaat ttgaagaagc agataaacaa ctccaagcac aagaaaaaaa
    66961 agttcaggag ctgcaacaag aaacagctcg tttagccgaa gaacaagaag ttttgcgtac
    67021 acgaggcgaa caattacgtt ttgaagaaca agaagcgaca aatcagctac aaaacatcat
    67081 aaatgaactt gaacgttttg aaaaagaaaa gcaaatctca acttttgaga cacgtgaact
    67141 gcagcagttt atcgaagatt acgaaaagca aacgaacgaa ttgaaagaca aacaaacaga
    67201 tcttgaatcg caacgacaaa aaatcgacga agaaatcaaa tcgttaagtc aagaaagcga
    67261 ccagatggaa gccagaagag cacaagtaca aagtcaaaaa gcacaagaac aagcagatct
    67321 tgctgtcttg aaagagcagt ttaatcatct gcagatccag ctccgcggtg cccgagtcca
    67381 aaaagcagag gcaaccgaac gtcaagaagc gatagaaaag cagttggcga cattgacagc
    67441 agacttttct gatcatgaag tcacagaaga aagtttggaa aaacaaatta acgaattatc
    67501 tgctcagcga gaaacattaa aagccgagtt ggcaaaagcc aaagagcagc gagatagaag
    67561 gcaaaaggag atcgataagc ttgaagcagt cttggcagag cgtaatcgtg agcaaaaagc
    67621 acgactgtcg gaacaatcca aattagaagt ccaaaaagac cgagctgaaa tgattttaga
    67681 taatcatttg acctatctgc agtcagaata tcagctgaca tttgagaaag cttcccaaga
    67741 ctatcaagaa acaaccgata tcgaagacag tagaacaaag gtttcatcct taaaagaaca
    67801 gattgaaaaa ctgggaccag taaacttaaa ttcaatcgaa caatacgaac aagtcagtga
    67861 aagacataca ttcttagcaa cacagcgaga tgatctgtta gcagcgaaaa accaattgtt
    67921 tgaaacgatg gatgagatgg atgatgaagt gcggacaaga ttcaaagaag tatttgaagc
    67981 tatccgtcaa gaattcaaaa ttgtctttcc aaatatgttt ggcggcggta aagctgaatt
    68041 agtactgacg gaaccaactg atttgctgaa aacaggtatt gagattgaag tacaacctcc
    68101 tggcaaaaaa ctgcaaagtc taagtttgct ttctggagga gaacgagcac ttacagccat
    68161 cgcgttgctc ttttccatta tccgtgtctg tcctgttccg ttttgtatct tagatgaggt
    68221 agaagcagcg ttagatgaag ccaacgtgaa acgttttggt cgttacttga gcgatttcca
    68281 agatgacaca caatttatcg tagtcacaca ccgcaaagga acgatggtcg cagcagatgt
    68341 actgtacgga gtgacgatgc aggaatccgg tgtatcaaaa atcgtttctg tacggatgga
    68401 agatatcaat gaagaggcga aaatcaacga aacgaaagtg taggaacgaa aatgatcaaa
    68461 ttagtagcaa tcgatttgga cggtacatta ttaaatgaac aaaagctgat ttcagatgag
    68521 aataaacaag cattagcaca agcaaaacag caaggggtca agatcgttct atgtacagga
    68581 cgccccttag ctgcgatggc gcattatctg caagaattag gactggtaga tgaaggtgac
    68641 tacagcatca ctttcaacgg cggtttggta caaaaaaatg atactggtga aatcatggaa
    68701 aagaaagtga tggaagtatc agatatccaa cgcttataca aactggctca gcaattggat
    68761 ctgccactag atgttttgtc tgacaatgtg gttttacagc tgcctagtgc cccaaggaag
    68821 caatccttgt acaatgtgtt gaataagctg ctgcaattcc aaccagcgga tataagcgat
    68881 attacagatg aatttgtgct taataaggca gtaatcgctt atgaccaaga agaattagat
    68941 cctaaaatca aagaaatacc agcagaattc catgaaatgt atgagatcat caaaacaaga
    69001 agtatgcttt tggaatttat gccaaaaggt gttacaaaag cttatggtat ttctttattg
    69061 gcaaaagatc ttggtttgaa acaagaagaa atcatggcga tcggtgatga ggaaaatgat
    69121 ttaccgatga tccaatatgc tggactaggc gtagcgatgg agaatgctgt gcctttcgtc
    69181 aaagaagcag cagatttcgt gacagccagc aatgaagaaa atggcgtagc caaagtgatc
    69241 caaaaatttg tattagagta gggggagtca aatgggattt tttgataaaa tcaagcgtgc
    69301 ttttactggc gaagagaaaa cagaagaagt tgaagtacag gagaagtacg acaagggttt
    69361 agaaaagacg cgtaaaacat ttggtgaacg gatgaatgag ttgtttgcca attttcgaac
    69421 agtcgatgaa gacttttttg aagaattaga agaaacactt atcggtgcag atgtaggttt
    69481 tgaaacagcg atacgaatca ccgaagcatt acgtcaagaa gtgaaactca gaaatgcgaa
    69541 aaaatcggca gaagtccaaa atgcgatcat cgagaaactt gtggatctct atgaagcgga
    69601 aggaatcaat gaagtaaatg aactaaatat ccaagaagac ggactaacag ttattttgtt
    69661 tgtcggtgtt aatggtgtag ggaaaacaac aagtattggt aaacttgccc atcagttcaa
    69721 aaacgaaggt aaaaaagtat tgatggcagc agcagatacc ttccgcgctg gagcaatcga
    69781 ccagttagtt gtctggggcg aacgcgctgg tgttgaagtt gttcgtggca atgcaggagg
    69841 cgatccagca gctgtcgtat tcgatgcttt ggaaagagca aagacagagc aggcagatgt
    69901 tttactagtt gatactgcag gccgactgca aaataaagtg aacttgatga acgaattaga
    69961 aaaaataaaa cgagtaatcc agcgggaatt accagaagca ccccaagaaa cacttcttgt
    70021 attagatgca accactggtc aaaacgcgat ggttcaagca aaacaattca aagaaacaac
    70081 agatgtgacc ggtcttgttt tgacaaaatt agatggtaca gcaaagggcg gtatcgtact
    70141 tgctatccgt aatgaattgc atttaccagt gaaattggtc ggtcttggtg aaggaatcga
    70201 tgacttggag ccgtttgatc caaatgattt tgtcgttgga ttgttcaaag gattactaaa
    70261 agaagaataa cccatgtcta aaaagcagga gctgtgacaa aagttgcagc tccttgtttg
    70321 aatgattgtt tttaacatag ctgtgttttt gatgccttca tttttatact tatttgtaag
    70381 cggttctcaa acaatttcgt tctttttctt agactttttg attataatta gattcatatg
    70441 aattggaggt cacttaatga tgaattttaa gcaattatta ttgcatgtca atgcgcgccc
    70501 ttttatcgac caagctcgat ttggtataga acgggaagga cagcgggttg atcttgcagg
    70561 aaatctagca aaaaccgatc atccagcaat ctttggcgat cgatcctatc acccctatat
    70621 ccaaacagat tttagcgaaa cacaaacaga gatgatcacc cctgttaccg attctattcc
    70681 cgaattattt cagtatctcg ctgctgttta tgatgtgact gctcgttcta taccgaaaga
    70741 agagatgatc tggccattaa gtatgccacc tgccttaccg gaaaaagacg aagagatcat
    70801 tattgcaaaa ttaaaaaatt tcgaagatgt cttgtatcga cgttatttag caaaagaata
    70861 cgggaagcga aaacagatgg taagcggtat tcatttcaat tttgaatttg gcgatgagtt
    70921 gctaagaaca ttgttcagcc atcaggaaga atttcaagat ttttccgaat tcaaaacaga
    70981 actttatttg aaaacagcga gaaactttat gcgctatcgc tggatgatca cttatttatt
    71041 cggtgcttca ccaatgagtg agaagaacta ctttttagat gaatcacatc cgcaagaacc
    71101 tgttcgcagt attcgcaaca gtgcgctggg ctatacgaat catccaaatg tgaaagtttc
    71161 ctatgcttct atgaaacagt atttagcaga tatcgagcga atgatcgaag aagggaaact
    71221 ttcggaggag aaagaatttt atacaccgct tcgtttccga ggcggaaaga aagtcgcaga
    71281 tttagcaaca acaggtgttc gttatattga attgcggaat atcgatttga atccttatgc
    71341 aagattgggt atcaatccag agcaagttcg gttcttacaa ttattcctga tgtacatgtt
    71401 atggacagaa gaaaaagaag actgtgacca atgggtagca gaaggtacga ctcgaaataa
    71461 caaagtagca ctagaacaac catcagatca aacagaattt catcaagaag gtagagaaat
    71521 tcttgaagga atgaagcaaa tgctggttga attggattgg ttggattctc tttatttagt
    71581 agaggaagca ttgactcaaa tggatcatcc tgaacaaacg ttagcagcga aactctatca
    71641 agaagcacag ctatcgagtc agcaagaagt tgctgtggca ttgggacatc aatattataa
    71701 agaaagccat gaacgtccct atcagttggc cggttttcgc gaaatggaac tttctactca
    71761 gatatttatg ttcgatgcca tccaaaaagg tgtccaagtc aaagtattag atgagtcgga
    71821 tcaatttttg cgtctgcaat tccaagacca tgtagaatat gtgaaaaatg caaacatgac
    71881 aagtaaggac agctatatcg tcccacttat catggaaaac aaaacagtaa cgaaaaaagt
    71941 cttaaaagaa gctgggttcc gggtaccagg aggcgcagaa ttttcatcca tggaagaagc
    72001 ggtaaaagct tatccgagat ttgccgagca ggcatttgtg atcaaaccta aatcaaccaa
    72061 ttacgggtta ggtatcacga ttttcaagga aggagctagt ctggaagatt atcaagcagg
    72121 tttagccatt gcttttcgtg aagacagttc ggttttagta gaagaattta tgccgggaac
    72181 ggaatatcgt ttctttgtga tcgacggaga agtccaagcc atcatgttgc gagtccctgc
    72241 taatgttatt ggtgacagta tccgaacagt gaaagaactt gttgaagaaa aaaatagtga
    72301 ccctttgcgt ggaacgaatc atcgtgcacc tttagaattg atccaattag gtgaattaga
    72361 acaattgatg ttgaaagaac aagggttgac gatcgaatca gttccccaag ccaatcaaat
    72421 tgtttatcta agggagaact caaatatcag tacaggtggt gattcgatcg atatgactga
    72481 tgaattttct gaagcttaca aaaaaatcgc cgtttctgcg gtagaagcac taggagctaa
    72541 gattagtggt atcgatttga ttataccaga taaagaaatc gatcctacaa ctgataaaaa
    72601 agcatatggg atcatcgaag caaatttcaa tccagccatg catatgcatg tttatccttt
    72661 tgcaggaaaa gacagaagat tgacgatgaa tgtattgaaa ttgttatatc ctgaagtatt
    72721 ttaaaagaaa aagcttttga cagattgtcc tatttgatgt caatctgatc aaaagctttt
    72781 tttgcatgta aaaatttatg tgttttttcg ttttaaaata aacgatccca gaagcttttt
    72841 ttcttttttg gtttttcttt ttcagctggc gaagagtttt cttctgggta ttttttttcg
    72901 atatcaatca caggttcatt tactgcttct tttgctgtta agagcaatcc gaattcctca
    72961 ggatttgcag tgacgtaatc gttgactaca gtgaaacggc tattgttttt cgttgctagc
    73021 tggatatatt ggttttggac agaaaggggt agagccccat tcaagagtat ggaagcatct
    73081 ggatgttttt tgagttcttt taaaaagttt tctttgtttt ttggttggat catttcagca
    73141 acagtcatac taagatagca gcgttccctg aatgtcccta aatatttatg ctgttcttca
    73201 gggttcacta aaggtgtacc ataaagtccc ttatccaaat ggctttttac atcatcttgt
    73261 gccataaaaa ataccctcct tcttctcttt gtctttctta cgattatagc atattcccaa
    73321 taatacctaa aacatatgaa aaagcggttg cttttgttca acgattcact taaaataaga
    73381 atgaaaatca ttctcaatta gaaaggtgga ttggatgcgc tacggacgac tagtagcatt
    73441 attagttatt ttaagtagta tttcattgtt catcggagta tcgaatgtac aattgtcaga
    73501 actgctccag ttagatgggc aaagcttaca aatcttgcta gtgactcgtt tgccacgaac
    73561 gatttgtttg atccttgtgg gagcgaccag tagtatttgt gggttgatca tgcaacattt
    73621 gacccaaaat aaatttgttt caccaacgac tgcgggcacg atggatagtg cgcgtttagg
    73681 tattttagtg gctatgattt tcctacctgg tgcatccttg attgtccgat ccttgacagc
    73741 attttgcttt gcttttgcag gaacattgct ttttttatcc cttacgcgat tgttcctcca
    73801 gaaaaatcaa ttgatgcttc cgttgattgg agtcatgtta gggaatatca ttggttcagt
    73861 tgctactttt tttgcttatc aattccagtt ggtacaaaat atgacttcat ggcttcaagg
    73921 gaattttgcg actgtgatac gaggaaatta tgagttattg tatgctgttg ttccattgtt
    73981 gctcttggtt tggctatttg cttaccgatt tacgatcatt ggtctgggcg aatcgtttgc
    74041 agtaaatctg ggaatagatt accagaagat gcagttttta ggcattggct tagtatcatt
    74101 ggctagtgca gtcatgttga tcatggtagg aagcattcct tttatcggag tgatcattcc
    74161 gaatcttgtc tctagaatat atggagatca agtgcacaaa acaattggcg taactgcttt
    74221 agcaggtagt ttgttcttag tattttgcga tcttgtcgct cgcgtgttga tttttcctta
    74281 tgaaatacca gtcagtgtga tcgttggaat catcggtggg gctatcttcc tttatcttct
    74341 gataagggga cgacgatatg cttaaaaaaa agccatctat ttttattggt atgcttgttc
    74401 ttgttttatt tgttacagga atgtacttga cgatccatac atatggaaac tggcattttg
    74461 catgtgcatt gaggagtaag aaaatttttg catttatcct tgtctcgcta gctgtcagca
    74521 gtgccacaat cagctttcag acaattacgc ataaccagtt tttgactcct tcagtattgg
    74581 gacttgagtc gttatatgtc cttgtccaaa cggtcctttt cttctttatc ggaggagtga
    74641 cgatgcttca gcagacaggt acagtttttt tcttattgaa cgttgggctg atggtcgggc
    74701 tgagtctatt gttatttttc tttcttttga aaaaaatgag agcgaatctt tttttattac
    74761 tgatgtcagg gatgatttta ggtacgcttt ttggtagtgt cagtaccttt ttacaagtca
    74821 tcatggatcc gaatgaatat gatttgcttc aaggcagatt atttgcaagc tttggcaata
    74881 tccagagtca gtatcttttg cctgcttctt tgatcattat cggctgctgg tacctgttat
    74941 ttcgatatcg acatgtgcta gacgttttcc atctcggaaa tgaacaagca gtaaacctcg
    75001 ggatagacac agatcgtttt gaatttttga ttttatgttg tgtcagtctt ttgacaggga
    75061 catcaacggc tcttgtgggt ccagttactt ttttagggtt tattgtcgca aatatcagtt
    75121 atcgtgtctt ttctacttat cgtcatggtc agctatttat cggaagcttt ctggtagcta
    75181 tgctgtttct gacaggagga cagctcgcag tagagcagat tttcggctgg aatacaacag
    75241 tgagtgtcgt cgttgaattt gtcggaggaa tttattttgt cacaaaattg attcgcgaaa
    75301 ggaagagtat cctatgattg atttacaaaa ggtcaataaa ctgtaccatg gaaaatcagt
    75361 ggttaaagat gtacagttaa cgattcctga aaagaagttg actgctttta tcggtccaaa
    75421 tggtgctgga aagtcaacgg tgttgtcgat gatcagtcgt ttgataccaa aagatacagg
    75481 tgaaatctat ttagaccata acgaagtaaa ggcttggaaa tccaatgaat tagccaaaaa
    75541 attatcgatc ctacgacaat cgaatgaaat aaatgtccaa atcaccgtga aagaattagt
    75601 agaatttgga cgatttcctt atacgaaagg aaaattgacg acaaaagacg aggaaatcgt
    75661 tcaggaatca ctggaacacc ttgggttaga agaaatagcg gatcaatcga ttgacactct
    75721 ttcaggcggt caactccaac gtgcgtatat tgcaatggtg ctagcgcaag acacggatta
    75781 tattttactg gatgaacctt tgaacaatct agatatgaat tttgcggttc aaatcatgca
    75841 gattttaaaa aatttagtac aagaaatcgg gaaaacaatt gttattgttc ttcatgatat
    75901 caactttgct gcaagttatg cagacgaaat tgttgcaatg aaagatggac agttatttac
    75961 gcaaggaacg acccaagaaa tgataaaaaa agatgtattg gatgaattat acgaaatgga
    76021 catacggatt tgtgagattg aaggccgcag attctgtctt tatttctaaa actaacatta
    76081 ggagagaaga gatgaaaaaa ttagtagtag gtatcttgat tctagcatca tttggtttag
    76141 cagcatgtgg aaatagcaac aacaccagtc aggcagacac aaaaaaaagc agcacacaaa
    76201 ctgaaactac attgacgata acagatagta atggagacca aatcgaggtg ccaaataatc
    76261 cagaaaaagt cgtcgtattc gacaatggtt ctttagatac gatggatgct cttggtgttg
    76321 gagataaggt agttggagca gctacaagca gccttcctga gtatctttca tcctataaaa
    76381 aagtagaatc agcaggtgga ataaaagaac cagacttaga aaaaattaat cagcttcagc
    76441 cagatctaat catcatttct ggccgacaaa gagattttca aaaagatcta agtcaaattg
    76501 ctccgaccat tttcctttct ttagatgcga aaaatccatg ggaatctttc cagcaaaatg
    76561 tcactgcttt aggggagatc tttggtaagc aagaagaagc taaaactcaa ttagaggaat
    76621 tatctagtgc aatcgatcag acgaaaaaga aagcagaagc tactgacaaa aaagcactag
    76681 tcacacttgt gaatgaaggg caattatcag cctacggatc tggttctaga ttcggtttca
    76741 tccatgattt atttggcttt gaacaagcag atgatcaaat cgaagcatcc actcacggtc
    76801 aaagtgtttc ttatgaatat gtgctggaaa aaaatccgga tatcttattt gtagtagaca
    76861 gaacaaaagc tattggtgga gacgacagca aagatgatat ttctgccaat gaactggtag
    76921 cacaaacgaa tgctggtaaa aatcagcaga tcatctccct tgaaccagat gtctggtatt
    76981 taagcggggg cggtttagaa tcaatgaaac tgatgatcga agatgtgaat caagcattta
    77041 aatagcataa ataacagagg attaaactct catactatgt aacaataatg tttcttacat
    77101 tcttaaatag caagagccta tcctcaaaga agttatcaaa aaccatccgt aaaatcgtat
    77161 tgatgatttt acggatggtt tttatttatt tgagaaatct tttttcaacg tgaattcatt
    77221 atctacatca tacgggttga ttctagaatg gggagtggca aaaaatagtg tgatccattt
    77281 tttcagcatc aagatcgcct cctttgattt gaatcaaagg tacaaagcga aacttagata
    77341 aaacttaaat ttagatattt ttattttgta ccgatctctt ctttaaacga tgttccatct
    77401 gctgcttctc ggcaataagt ccactagact agaaaatatg agaagctatt ttctgtccag
    77461 cgttcttatt actcaaaaca aatcgcagtt gtcacaatct cttctaaacg atgttccatc
    77521 tgctgcttct cggcaataag cccactagac tagaaaatat gagaagctat tttctgtcca
    77581 gcgttcttat tactcaaagc aaatcgcagt tgtcacaatc tcttctaaac aatgttcaat
    77641 cagctgacct tcggtattag ctcaaagcac tgatttcaca acctctattt aggcatgtgt
    77701 ctcagcaagc ggttgatagt tgatgatctg catttttcga taaaaatcgg cgggaagctt
    77761 actccaccgt tttagtaatt gatagaaata actctcttct gctttgattt tatactcgct
    77821 taatatcaat ccaaaactat tcgttttttt tgaagctaat tttttataaa tatccaatgc
    77881 tttggtttta tcttcattga caaagagtgt ttttacttca atataagaac cctcgatatt
    77941 ttgtgtttct tcgataaaat agatgttttt tatatattcc ttcatagtga ttcctccaaa
    78001 gaagtctttt atttgataag tttactctac cgaagagttg ttaataatcc gtgaacaaaa
    78061 agtaattctt tttccaatag ttgttaaagg ttttttattt tttatagtat tttttgttat
    78121 ttaagaaagg aataaggtta aacaaataac aggttgcttt ttgggtcttt ttccgttcaa
    78181 gaaactgaac gaagaaaagg gaaagttgtc tcttttgtga taagagaaat cataataaac
    78241 gaatgactta ctttccaata aattgagaac agtcagaacc agacaaatta gttgcatctg
    78301 atgaataaag tgattattct atgacatgag gtgatacaga tgaaattaga agtgacaaaa
    78361 gaagcacaag aagtattgaa gaataaatta gaagcagacg atcaactttt attagatatc
    78421 gaaaacggtg acggtccatt tgcagaaagc caagtcagtt gccaattgga tacggcattt
    78481 cgattgatca ttgtcaaaaa agatacacaa acagatctaa gtctttattc tgtagtggct
    78541 gatacaccag ttggaccaat caaaatcaaa gacagtgcaa tactttacat ggatgatcca
    78601 tccacacttg ctttagaacc aacttataat agtttacaat taaaaggacc aagcggcttg
    78661 agaaaaggca acttgcaagt tgttcgaaaa gactagaggg ataaaatggg aaaattggcg
    78721 atcatcagcg acctacatgt ggacatcaat aaattcggcg aatcagagct gatgcagctt
    78781 tggcaagttt tgcaaaagca caagatcact cggcttcatc tagccggaga tacggctaat
    78841 aaagtagacc gttgcctagc tgttgtcgaa tttttcgtca ataaaggatt atctacgaca
    78901 tttcatttcg gcaatcacga gttggcagat gtgcccaatg aacagatgat ggaccatttt
    78961 ccagacccac attttttaaa tgaacggtat attgctttaa atgagcagac cgttctttta
    79021 gggatgaatg gctggtacga ctatcagttt tccgaattga aagattacga ggcaatcgta
    79081 cgaatgaagc gtttgtattg gtatgatcgg ttcatccagc gtgggaaaac agatctcgag
    79141 gtcaatgcag aaatgcttgc agctgcaaaa aatttgctag accagctgga agcaaaaaaa
    79201 cttgaggtga ttttatccac tcatttcgtt cctaaacggg catttatcat gtatcaatct
    79261 gctccttatg agcgttggaa caaattaaat gcatttttag gatcagaatc attcggagaa
    79321 ttacttgacc gttattcaaa tatcaaacaa gtagtttttg gccatacgca tcgtcgcttt
    79381 gaagataaat ccatcggtca cacgatttac agctgcagac cttttggtta ttattatgaa
    79441 tggcagctga cccgagattt tgtcttgaaa gaacagttgc tagacagcta tgatcctatg
    79501 aaattacgtg tcctcttgcg tgcccatcac ccagcttttc aggcctatca aacacaacat
    79561 ttgcaagagg aatttgaaca agcgatgaca atcgttccgt attgagacag cattagtaat
    79621 gtgtaaaaga aaataccttg taaaacataa ggataagtaa gagcagtaga tccatttatc
    79681 gtggatatgc tgcttttttt gcggaaaata atcagcgaat gtttatttga tcggatgatt
    79741 tgtaactcgc agagtactag atgcaagcgt gtattctttt tttgaacgat aatcacaaaa
    79801 aaaaatataa tatacatttt ttatttaatt ataattaata ttgattgaag tctaggcagt
    79861 caagtcacat tttgatagat aggaggggga cttacgaatt attttaaagg ctatgacagc
    79921 tgtatttcta gattgtgttt gcatcaagtt gcaataaaaa ctagaaatca aatcgggctg
    79981 ataatcattt ataactaatc gatacatagg accaaatata ggtctatgtt cttttatttc
    80041 ataagtatcg taaaacagaa ttttgctatg gattaatcca atcgactgat atgtttagta
    80101 aataaagaaa gaggaatgtc aatgatgaaa aaagaaatcg tattaacagc agcaatgatg
    80161 ttattcagca tggtagcaag tacgactttt gtttctgcaa ctgaagtata cccaaaggaa
    80221 tataacacag aaggaacgat tacatttgaa gcaggagatg agggtgtaac tcctcctgtt
    80281 gatccagaga atcctgatcc caataaacct gtcgatccat cagatcctcc aagccctgga
    80341 acaggagggg cattatcgat tgattatgga tcaaaattca aatttgggac tcaaaaaatt
    80401 tctactgcgg ataaaacgta ttatgcagct gctgatgtga tgaacgatgg gtctagaaaa
    80461 ccgacatatg tccaagtgac agataggaga agtaccttat ctgggtggaa attaagtgtc
    80521 tctcaaccag aacagtttaa aactgccagt ggggatgaac tagttggagc acagctgaaa
    80581 tttacgaaag ggcaggctgt ttctttggtt gatccaacat atacgccgca aacggtgaat
    80641 tctgaattaa cgttgacacc gggaggaaac aatacgttag caattaatgc taaatcagga
    80701 acaggtgtgg ggacttgggt ctatcgattt ggtgcaaatg aaaatgaaaa tcaagatgcg
    80761 gtacagcttt cggtacccgg taaatctgtc aagttagcgc aacaatattc cactaaatta
    80821 gtttggacat tagaagatac acctaacaac tagtttactg aaaagaactg ttaaaaataa
    80881 atacgtagtc atattgtctt gcgaggtaat cgagcaaaaa ttgtcaaagg ctaggctaaa
    80941 tttgtttgga tacgaatagt tcatctagga atttaacgat caggaggaaa agatatattg
    81001 aaaaagagga ggatcgtgct cattttaagc agtctcttag tgactaatat ctttccgatc
    81061 atagggtact ctttggaatt gaatcagttg gagcatggaa atagaagaga cgaagaaagc
    81121 aaactcactt cgacattcga cagtttaata gaatccaacc agacacaaga aagcagtgag
    81181 gatttaagta tttcgagtag tggagagacc actgacaacg tagaagctga gggttcggac
    81241 aaacaaggac tagaagatgc accaatttct gcggttgagc tgcctgacga tcccaatatt
    81301 atttctatcg ataaaatttt tcaaacacct atcggtacta gcacgtccat attggaaaat
    81361 gggaagttac tgcagttgaa cccagcagaa aggtctcaaa gaggagctat ttggtcaaat
    81421 aagaagatta gtttactctc ggattttaca tttaaatctt atttatactt aggaaatcaa
    81481 cgagatagag cgggagatgg catgaccttt acattgactg gcgatcctag aatggctagt
    81541 aatcccagtc aagttattgg atcgccaggt atgggaattg gctcatattc tactcgttct
    81601 ggccaacctt acattgcgaa tgcgctctcg attgagtttg atacgtataa aaatagtggt
    81661 tcttctgatc gaatggatcg agagataact gctgataata gagggcatgg acatgtcgct
    81721 tttgtcacgc ctaaaccaaa taataataat tattctggag agcatactgg cgtgacggta
    81781 gctcctacat ttctttctaa tggcacatgg cgcttgttaa taattaaatg ggacgcggag
    81841 caaagaagtc ttacttatga tttggaaggt gtcggtcgct cttctcggat tatacctgat
    81901 ctcattaggc agtttggttc tacagaagta aattgggggt ttacttcttc aacaggtact
    81961 ttttttcaag aaaatgctgt cgctgtcacg cgacttccat cgaatgtcac atcaacagct
    82021 gatgtaaaag tcaacgatag tgattatggg gccatcgctg aagctggagg aaacgataag
    82081 ataactatca gaaatacatt gaaggtcgag ggggattttc ttgaatataa gaaaatgcag
    82141 actagtatcg acctcccaca agaattaact attcccgagc atatcttgat caatggaaat
    82201 caaattgatg ccaaagatat cttgataacc ggaaatcgag tcacgctaaa tctgacagat
    82261 tattttattg ctggagatac gttaataatc gaattggaga caactttgca ggaagtaatt
    82321 cctgaaaaag tactcactat gcattttgaa taccttgaaa acgggaattt gattgagcaa
    82381 tcgaatgaaa taaacctaaa gatcgcaaag ctgaaggaga tagatatcca tgtttattat
    82441 aaagataaag acagtcagat tgatattgct gaaatgaaaa caatcactgg gaaaatagga
    82501 gagaagtata aagaaactcc tattgatatt gaagggtatg tttttgactc ggattcagcg
    82561 aatacagaag gcatcttctc cgaaaataca gaggatatct atttttatta cagaaaaggc
    82621 aaactttatc tctcaactgc tcctcaatat gtcaattttg gaaaacataa aatatccagt
    82681 agaccattaa gtgaattcgg acagttttca ggtgggttga aaattataga tgaacgggct
    82741 tttggaagtt ggaaattaca attgaaacaa gagaaagttc ttacaaatgg agattttgag
    82801 cttccagaag ctctttcctt tgttacctct gaaaatacaa cggttatagg gacatccgct
    82861 gttacgattt tcgaaagtga tcaaaagggt gaaagcgatt tgtcctctct tttagattct
    82921 acagctcatc gtggaatcag aataaatatt cctgtagaaa accaaagaat cggaaccttt
    82981 gaggggaaac tatcttggat acttgcagat gtcccaggaa attaacagga agagaagaat
    83041 ataacaaaat tattgagttt caagaagggc acaggtacca taggctcttg tgtcccacag
    83101 agacaagatt ttgttatagt cttctcttac atatgattaa aggagcgatt ttcttgagac
    83161 caaaacaaca attatttata acactcctag ttattttagg ggcacttttt aacaatatat
    83221 cagtgtcagc tagtgcattt aatttttcag tgacgccgat aacttcagag aatcaaatcg
    83281 acaagagaaa aacttacttc gatcttcaac ttgatccgga tcaggaagta gaagtaaaag
    83341 cggaattgag aaatgataca gaaaaagaag tgaaaatcga tatttctgtg aatagcgcaa
    83401 ccaccaattc aaatgtgatg gttgaatacg gaaaaaacga gattgaaaaa gacatgagcc
    83461 tgatctttga tctggtagat tatgtgagct atccacagat agtgacgcta aaacctaaaa
    83521 gtgtccaaac cgttgctttt catgtcagga tgccgaatga gcgatttgat ggtgtattgg
    83581 ctggtgggat cacattccaa gaaatccaga cggaaaagga ccaaacagaa actaaggatc
    83641 aaagcttatc gattgagaat gaatacacct atattattgc tttgttgatg caacagacac
    83701 tgaacgaagt tgctccgaac cttactcttc atgaagttaa accagatcaa atcaatgcaa
    83761 gaaatgtcat cttagcaaac gttcaaaatg atcaaaaaac ctatatcaat caagtagtta
    83821 ttgaaacaaa aattacaaaa aaaggacatt ctgaagtttt atatcaagag gaaaaagaag
    83881 gattgcagat tgcgccgaat acaaattttt cttttccaac tgctttaaat ggtcaaccgc
    83941 taaccccagg tgaatatcac cttacaatga ctgttttagg aaatgaaaat gaaaatggaa
    84001 aattttcgag aaaaaaagga aatactacga tcaactatac caatcaatgg gtatttgaaa
    84061 aagattttac aatagacgga aaagtagcca aggaattgaa cacgacggat gttacgatta
    84121 aaaaagacaa taattggatt tatatcttag tagcttcact tttcttattg tttattttat
    84181 tatgcatact tttgtttgtt ttgagaaaaa aagaagagaa agaaaagtaa ggttaagata
    84241 acaaatagta aatttttat
//
LOCUS       DACDEG010000003        76614 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.003, whole genome shotgun sequence.
ACCESSION   DACDEG010000003 DACDEG010000000
VERSION     DACDEG010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 76614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.003"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..231)
                     /locus_tag="IV142_000207"
     CDS             complement(<1..231)
                     /locus_tag="IV142_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8483898.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMK"
     gene            complement(331..840)
                     /locus_tag="IV142_000208"
     CDS             complement(331..840)
                     /locus_tag="IV142_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAP8483899.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(925..1404)
                     /gene="rlmH"
                     /locus_tag="IV142_000209"
     CDS             complement(925..1404)
                     /gene="rlmH"
                     /locus_tag="IV142_000209"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP8483900.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            1832..3121
                     /locus_tag="IV142_000210"
     CDS             1832..3121
                     /locus_tag="IV142_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP8483901.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(3160..3765)
                     /locus_tag="IV142_000211"
     CDS             complement(3160..3765)
                     /locus_tag="IV142_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP8483902.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(3970..4434)
                     /locus_tag="IV142_000212"
     CDS             complement(3970..4434)
                     /locus_tag="IV142_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8483903.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(4456..4776)
                     /locus_tag="IV142_000213"
     CDS             complement(4456..4776)
                     /locus_tag="IV142_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP8483904.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(4920..5999)
                     /gene="pepA"
                     /locus_tag="IV142_000214"
     CDS             complement(4920..5999)
                     /gene="pepA"
                     /locus_tag="IV142_000214"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP8483905.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            6199..6549
                     /locus_tag="IV142_000215"
     CDS             6199..6549
                     /locus_tag="IV142_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8483906.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            6772..7719
                     /locus_tag="IV142_000216"
     CDS             6772..7719
                     /locus_tag="IV142_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8483907.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(7776..9194)
                     /gene="pepV"
                     /locus_tag="IV142_000217"
     CDS             complement(7776..9194)
                     /gene="pepV"
                     /locus_tag="IV142_000217"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP8483908.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            9343..10188
                     /locus_tag="IV142_000218"
     CDS             9343..10188
                     /locus_tag="IV142_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP8483909.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            10192..10632
                     /locus_tag="IV142_000219"
     CDS             10192..10632
                     /locus_tag="IV142_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP8483910.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            10682..10876
                     /locus_tag="IV142_000220"
     CDS             10682..10876
                     /locus_tag="IV142_000220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8483911.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            11012..11698
                     /locus_tag="IV142_000221"
     CDS             11012..11698
                     /locus_tag="IV142_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8483912.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIRRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(12106..12795)
                     /locus_tag="IV142_000222"
     CDS             complement(12106..12795)
                     /locus_tag="IV142_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8483913.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(12770..14899)
                     /locus_tag="IV142_000223"
     CDS             complement(12770..14899)
                     /locus_tag="IV142_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP8483914.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(14947..16284)
                     /locus_tag="IV142_000224"
     CDS             complement(14947..16284)
                     /locus_tag="IV142_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP8483915.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(16536..16895)
                     /gene="rplT"
                     /locus_tag="IV142_000225"
     CDS             complement(16536..16895)
                     /gene="rplT"
                     /locus_tag="IV142_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP8483916.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(16948..17148)
                     /gene="rpmI"
                     /locus_tag="IV142_000226"
     CDS             complement(16948..17148)
                     /gene="rpmI"
                     /locus_tag="IV142_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP8483917.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(17220..17744)
                     /gene="infC"
                     /locus_tag="IV142_000227"
     CDS             complement(17220..17744)
                     /gene="infC"
                     /locus_tag="IV142_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP8483918.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            18064..18696
                     /locus_tag="IV142_000228"
     CDS             18064..18696
                     /locus_tag="IV142_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP8483919.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(18698..19603)
                     /locus_tag="IV142_000229"
     CDS             complement(18698..19603)
                     /locus_tag="IV142_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8483920.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(19688..20341)
                     /locus_tag="IV142_000230"
     CDS             complement(19688..20341)
                     /locus_tag="IV142_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8483921.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            20663..21607
                     /gene="mvk"
                     /locus_tag="IV142_000231"
     CDS             20663..21607
                     /gene="mvk"
                     /locus_tag="IV142_000231"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP8483922.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            21600..22577
                     /gene="mvaD"
                     /locus_tag="IV142_000232"
     CDS             21600..22577
                     /gene="mvaD"
                     /locus_tag="IV142_000232"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP8483923.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            22590..23675
                     /locus_tag="IV142_000233"
     CDS             22590..23675
                     /locus_tag="IV142_000233"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP8483924.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            23672..24736
                     /locus_tag="IV142_000234"
     CDS             23672..24736
                     /locus_tag="IV142_000234"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP8483925.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(24985..25788)
                     /locus_tag="IV142_000235"
     CDS             complement(24985..25788)
                     /locus_tag="IV142_000235"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP8483926.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(25808..26650)
                     /gene="kduI"
                     /locus_tag="IV142_000236"
     CDS             complement(25808..26650)
                     /gene="kduI"
                     /locus_tag="IV142_000236"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP8483927.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(26666..27673)
                     /locus_tag="IV142_000237"
     CDS             complement(26666..27673)
                     /locus_tag="IV142_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP8483928.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(27680..28324)
                     /locus_tag="IV142_000238"
     CDS             complement(27680..28324)
                     /locus_tag="IV142_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP8483929.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(28424..29182)
                     /locus_tag="IV142_000239"
     CDS             complement(28424..29182)
                     /locus_tag="IV142_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP8483930.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(29309..30460)
                     /gene="fucO"
                     /locus_tag="IV142_000240"
     CDS             complement(29309..30460)
                     /gene="fucO"
                     /locus_tag="IV142_000240"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP8483931.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(30542..30853)
                     /gene="rhaM"
                     /locus_tag="IV142_000241"
     CDS             complement(30542..30853)
                     /gene="rhaM"
                     /locus_tag="IV142_000241"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP8483932.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(30855..31700)
                     /gene="rhaD"
                     /locus_tag="IV142_000242"
     CDS             complement(30855..31700)
                     /gene="rhaD"
                     /locus_tag="IV142_000242"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP8483933.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(31714..32970)
                     /gene="rhaA"
                     /locus_tag="IV142_000243"
     CDS             complement(31714..32970)
                     /gene="rhaA"
                     /locus_tag="IV142_000243"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP8483934.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLEEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(32967..34427)
                     /gene="rhaB"
                     /locus_tag="IV142_000244"
     CDS             complement(32967..34427)
                     /gene="rhaB"
                     /locus_tag="IV142_000244"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP8483935.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            34580..35449
                     /locus_tag="IV142_000245"
     CDS             34580..35449
                     /locus_tag="IV142_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8483936.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(35575..36474)
                     /locus_tag="IV142_000246"
     CDS             complement(35575..36474)
                     /locus_tag="IV142_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8483937.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(36639..37463)
                     /locus_tag="IV142_000247"
     CDS             complement(36639..37463)
                     /locus_tag="IV142_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8483938.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(37474..38769)
                     /locus_tag="IV142_000248"
     CDS             complement(37474..38769)
                     /locus_tag="IV142_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAP8483939.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(38785..39264)
                     /locus_tag="IV142_000249"
     CDS             complement(38785..39264)
                     /locus_tag="IV142_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP8483940.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(39270..40298)
                     /locus_tag="IV142_000250"
     CDS             complement(39270..40298)
                     /locus_tag="IV142_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP8483941.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(40315..41421)
                     /locus_tag="IV142_000251"
     CDS             complement(40315..41421)
                     /locus_tag="IV142_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP8483942.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(41650..44247)
                     /gene="adhE"
                     /locus_tag="IV142_000252"
                     /gene_synonym="adhC"
     CDS             complement(41650..44247)
                     /gene="adhE"
                     /locus_tag="IV142_000252"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP8483943.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(44552..44932)
                     /gene="yajC"
                     /locus_tag="IV142_000253"
     CDS             complement(44552..44932)
                     /gene="yajC"
                     /locus_tag="IV142_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP8483944.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(45029..46174)
                     /gene="tgt"
                     /locus_tag="IV142_000254"
     CDS             complement(45029..46174)
                     /gene="tgt"
                     /locus_tag="IV142_000254"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP8483945.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            46392..47090
                     /locus_tag="IV142_000255"
     CDS             46392..47090
                     /locus_tag="IV142_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP8483946.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(47258..49897)
                     /locus_tag="IV142_000256"
     CDS             complement(47258..49897)
                     /locus_tag="IV142_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8483947.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(50516..51547)
                     /gene="queA"
                     /locus_tag="IV142_000257"
     CDS             complement(50516..51547)
                     /gene="queA"
                     /locus_tag="IV142_000257"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP8483948.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            51740..52915
                     /locus_tag="IV142_000258"
     CDS             51740..52915
                     /locus_tag="IV142_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8483949.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            52919..53560
                     /locus_tag="IV142_000259"
     CDS             52919..53560
                     /locus_tag="IV142_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8483950.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            53557..54474
                     /locus_tag="IV142_000260"
     CDS             53557..54474
                     /locus_tag="IV142_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8483951.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            54471..55142
                     /locus_tag="IV142_000261"
     CDS             54471..55142
                     /locus_tag="IV142_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8483952.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(55683..56171)
                     /locus_tag="IV142_000262"
     CDS             complement(55683..56171)
                     /locus_tag="IV142_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP8483953.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(56372..56638)
                     /locus_tag="IV142_000263"
     CDS             complement(56372..56638)
                     /locus_tag="IV142_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP8483954.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(56638..56901)
                     /locus_tag="IV142_000264"
     CDS             complement(56638..56901)
                     /locus_tag="IV142_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8483955.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(56894..57166)
                     /locus_tag="IV142_000265"
     CDS             complement(56894..57166)
                     /locus_tag="IV142_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8483956.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            57377..57562
                     /locus_tag="IV142_000266"
     CDS             57377..57562
                     /locus_tag="IV142_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8483957.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(57704..59527)
                     /locus_tag="IV142_000267"
     CDS             complement(57704..59527)
                     /locus_tag="IV142_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP8483958.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(60125..62830)
                     /locus_tag="IV142_000268"
     CDS             complement(60125..62830)
                     /locus_tag="IV142_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP8483959.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(63136..64083)
                     /locus_tag="IV142_000269"
     CDS             complement(63136..64083)
                     /locus_tag="IV142_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP8483960.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            64273..64542
                     /gene="rpsN"
                     /locus_tag="IV142_000270"
     CDS             64273..64542
                     /gene="rpsN"
                     /locus_tag="IV142_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP8483961.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(64718..65080)
                     /locus_tag="IV142_000271"
     CDS             complement(64718..65080)
                     /locus_tag="IV142_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8483962.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(65091..66212)
                     /locus_tag="IV142_000272"
     CDS             complement(65091..66212)
                     /locus_tag="IV142_000272"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP8483963.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(66228..66578)
                     /locus_tag="IV142_000273"
     CDS             complement(66228..66578)
                     /locus_tag="IV142_000273"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP8483964.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(66708..68219)
                     /locus_tag="IV142_000274"
     CDS             complement(66708..68219)
                     /locus_tag="IV142_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8483965.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(68541..69893)
                     /locus_tag="IV142_000275"
     CDS             complement(68541..69893)
                     /locus_tag="IV142_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP8483966.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(70046..71122)
                     /locus_tag="IV142_000276"
     CDS             complement(70046..71122)
                     /locus_tag="IV142_000276"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP8483967.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            71318..73063
                     /locus_tag="IV142_000277"
     CDS             71318..73063
                     /locus_tag="IV142_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP8483968.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(73343..73780)
                     /locus_tag="IV142_000278"
     CDS             complement(73343..73780)
                     /locus_tag="IV142_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP8483969.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(73764..75137)
                     /locus_tag="IV142_000279"
     CDS             complement(73764..75137)
                     /locus_tag="IV142_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8483970.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(75459..75947)
                     /locus_tag="IV142_000280"
     CDS             complement(75459..75947)
                     /locus_tag="IV142_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8483971.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(75969..76571)
                     /locus_tag="IV142_000281"
     CDS             complement(75969..76571)
                     /locus_tag="IV142_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8483972.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        21945 a        16399 c        14003 g        24267 t
ORIGIN      
        1 tttcatgtca aatttaatcg gctgttttat ttttgtaaat tcgcttctac tactaatcca
       61 atccgagatt aatttttcaa catgtcccag agtaaactct gatttattcg ctaaatactt
      121 tttttgttgt attaaagaca tctttccccg aagctcaaaa ttgagcctac atcgattctc
      181 tatctcttca aaaagcatct tatcttcatc attcttatat tcatcattca taaaaccaat
      241 ccagctcctc gattaagata atattttaat ctcatacaaa aactagctct catcaaaaca
      301 accattgttt tgatttctca tgaaatttct ttatactttt ctccgattat aaattcctaa
      361 ccccttgaga aaattacgca tatatttatc tccgcaaggc ttaaaatttc gttggccttc
      421 ttttctcaaa aatgccccga tttcgccttt tgatggatag atttctgcct cagcaaaaac
      481 gtctaaaata tcatcagtgg tcatagacaa ggcgattttt aattttttta gcaacacatt
      541 gttgatatat ttgatttcct tgcttttttg cggcgtgtct actatcggct tatctttttg
      601 cggaccacgc gccaagagaa tcaggccatt taggaatgcg tcaaaaactt ggttgttgat
      661 ggttttttca tacacattat tttctgcatt atctttgttc ttatcctgta ctttggtcag
      721 taccgcctga gcttcttctt tagtcagtgg tacaccgcct agctcaaaag ctttaatcaa
      781 atccgtatct ttcaaatcaa tcgcataacg caatcgcaat aacctatcat tgttattcat
      841 ttgttcctcc gaagtattta gtctttactt tcttacgtac tttttgtctt gctaagattt
      901 ttaggatcaa tgataaaaac cgcatcactt atgataaggc gcccccgcat tgatccggaa
      961 ggcacggtag atttgctcga caaggatcag acgcatcaat tgatgtggat acgtcatttt
     1021 tccaaaagag atctgagcat tgcttctttt catgacggct tcgctgagtc ctaaagagcc
     1081 gccgataaca aaaacaaggt ggctttttcc ttggatacct aattggtcga tttgtttagc
     1141 aaaagattca cttgtaggat ttttcccttc aattgctaag gcatagacgt attcttgttc
     1201 tttgattttc gctaagatcc gttccccttc tttttctttt acttgtctca tttgcgcttc
     1261 gcttaaattc tccggggctt tttcgtcagg aacttcgatc aattcgattt ttgcataggc
     1321 attcagccgt ttgacatact cattgatacc ttggatcagg tatttttctt ttaattttcc
     1381 tactgagata attttgatat tcatgttttc tctcctttgg tcttcatgct cactttgttt
     1441 attataatca attttttcgt ctatatgttt attttggcta tttttgttaa aaagatttcc
     1501 ccaacagtta tgcacaacat ccacaggttt atccacagaa aacagtgtta ttttacctta
     1561 tttcatcaca ttcattaggc aaacatacgt tttataagaa tttatccact ttatacacag
     1621 gctgtggata tgttttatcc cttatttttc caatcctatc cgcgtagttt tacacagtat
     1681 ctgtggaaaa agaaaatcaa atattttcat ctgaaaacaa tgatttttcc taaagttttt
     1741 cataaaaagt tcacagtctc aaagtagact aaagtcaaca aataagacta tactaacagt
     1801 agataaatga atgtaattgg aggaggaaca catggataga aaaaatgtga caccaaagat
     1861 gaaaaaaagt aaaaataata gcctctggcg taagttaggt ctggggcttg ttggagggat
     1921 cgttggagga ctactgacag ctggcatttt ttatgcagcg atgggcactg gcaacaatag
     1981 ttctactgct acaagcggca atcagaattc tgccggtgaa acagttgtgg aaaatgtcaa
     2041 agtcaatgta gattctgata tcaccaaagc ggtagataaa gttcaagacg ctgtcgtttc
     2101 tgttatcaac ttacaaagtc aaaaccaatc aagcggtttt ggcggactat tcgggcagca
     2161 agaagaaagt agcagctcag atgatagtaa tttggaagct tatagtgaag gaagcggcgt
     2221 gatctacaaa aaagacggga atactgctta tgtagtcacg aataatcacg tagtagatgg
     2281 ccagcaaggt cttgaagtct tgatgaaaga cggtacgaag gtaaaagctg aattagttgg
     2341 aacagatgct tattcagatt tagcggtgtt gaagatcaat tcagacaaag tcgaaacagt
     2401 tgcctctttt ggtgactcaa gtgctctgaa ggttggtgag cctgcgattg cgattggttc
     2461 cccactagga tctgaatatg caaattctgt cacttcagga atcatttctt ccttgaaccg
     2521 acaagtgaca agtacgaatg aatcgaacga aacagtcaat atcaatgcga tccaaacaga
     2581 tgcagcaatc aaccctggaa attctggtgg tcctttagtt aatatcgaag gacaagttat
     2641 cggtatcaac tcaagtaaga tcgcaagtac ttctgaatct acttctaatg taagcgtaga
     2701 aggaatggga tttgctattc caagtaacga tgtagtgaat atcatcaatc aactggaaaa
     2761 agacggaaaa gtgacacgtc cagctctagg tatcacaatg gtcgacttat ctgccgtttc
     2821 tactcagcaa caagaacaaa tcttgaagat tccttcatct gtaactaacg gtgtcatcgt
     2881 gacaaatgtc caaacagcta caccggctga aaaagctgga ttgaaacaat acgacgtcat
     2941 tactaagatc gatgacaaag aagtttcttc tggtgtagaa cttcaatctg ctttgtataa
     3001 gaaaaaagtg ggagattctg tcaaaatcac ttactatgaa ggctcgaaag aaaaaacaac
     3061 tactgttgaa ttgacgatcg atcaatcggc attaaaacaa aatcaaaata gtgaaaatta
     3121 ataaaaaaga ggttgtgaca aagacttgtc acaacctctt tattttggaa atgcttctcc
     3181 gactacggca tcctcaggca atactaagat tccttttttt gctggagcat ttggcaaatg
     3241 caattcacga gcggaacaga tcattccata gctatctacc cctcgcaaag ttcctggcca
     3301 gatcatcatt ccatctggca tcatagctcc tggttttgca acgacaactt tttgtccagc
     3361 ttcgatattg ggtgcaccgc aaacgatttg caatactgta ccattgtcta cttctgtctc
     3421 agtaatcgat aaatggtcag aatcaggatg tgggacgcat gtttttacgt acccgacaac
     3481 gatcttcggt tcatgatcag cttcaagctg ttcttcgaat cctgcttctt tgatcaatcg
     3541 attcaatttc gctaattcct catctgttaa gcttacttgg ccgttccctt cgattgttag
     3601 atgatcggag atatggaaaa agttccaagc aacaacttga ccttgttcat ctttgatccg
     3661 agcaacattc catttgcgtt caacagtgtt ttctgttcct ttatcgtcgg ctataataac
     3721 catcaaggtg tctccgacgt gtgctttgtt ataagcaaaa atcatgttct ttcctccatt
     3781 atcctttgct gagatttatc ttaacaaaaa attgcattca ctgcatgaaa tttttttcat
     3841 gctttatttc cgaagaagcc tcttctggaa caccatttat tcgtaacaag ggactgtgac
     3901 aagacttttg tcacagtccc tttactgttt tagttaattg attaaaaagg tcgtacaagt
     3961 tccacttgct taattacttg gctgttcttc ttcgtcagaa acgatcaaga catcacaagg
     4021 tgaatgacgt atcacgtagc tggctacact tcctgtcata aagcgttcga ctgcatttag
     4081 acctgactgc ccaaccatga ttagatcgat ctcatatttt tttggcaatt tgactgccat
     4141 ggattcacga acagaaccat atgcaataat tccttctata tttgtgtaat caacagattt
     4201 cccatattct ttacattctt cgatcatttc cttggctgtt tcagtttgct gatcgactag
     4261 attttgatca ataggtgcat agcccataaa gttataaaat tgctgatcga ttacgtttgc
     4321 tacatatact ttcccattat tacggcgagc cacttctacc gcttttttga aagcagcaag
     4381 tgcttgttca ctaccgtcga tacctactaa aatattctta tattcttgat tcatcttgac
     4441 ccctcctaac agatgtcagc cttttgttac actttctagg aacgattcga tttcctgttt
     4501 tgttttacga tctttattca ctagacgacc taattctttt ccgtcttgga tcacgacaaa
     4561 acttgggata ccaaaaatgt tccattcagc ggctacatct atgtacttgt cacggtctac
     4621 ttcaataaat tgccaatcag aaaagtcatt ttcaatttca ggcatttgcg gtttgataaa
     4681 acggcagtct ccgcaccagc cggcagtaaa gaaaaataca ctctttccag tttctacgta
     4741 gcctgctaat tcttctaaac tatttgggat aatcattctt tgtcctctac tttctgttta
     4801 tgtatttttc aattaaatta tacacctttc tttcgaacta acaaacattg atcctctttt
     4861 tataaaaaaa atctggcaaa aagcaatctt ttgccagatt ttatagtttc actatcgcat
     4921 tattttccgt atacgatcgt atttactgtt gatttatcca aacctttcag tgtttgaatc
     4981 aacatttctc gtgcagcttc aaaatcgcga atactgaaca tcgtttgatg cgtatggata
     5041 taacgtccca ctacgccaat cactgtactt gggattcctt cgttttgtgt atgagcagca
     5101 cctgcatctg taccgccttt tgaaacaaag tattgataag ggatattgtt tgtttcagcg
     5161 atatccagca agtactcgcg caaacgagga agcatgatca aacctggatc ttggatacgc
     5221 atcaatgtac cttctcctaa atgtccgaat gtgccatttt tcgtcactgt atcatctgct
     5281 gcagagcagt ctactgcaaa gaacagatct ggcttgaatt tattgacaga agctttagaa
     5341 ccacgcaagc ctacttcttc ctggacattc gcgcctgcaa tcaatgtatg acctaatgtt
     5401 tcattttgca acgcttctaa agcttctaat accattgtac agccatagcg gttgtcccat
     5461 gatttactga taatattctt tccgttggct gttttgatcg tttcagcgta aggaacgatt
     5521 gtgtctcctg gaaggacacc gtaactcatt gcctcttctc ttgattcaaa cccagcatcg
     5581 aacaaaatat cggttacttc tactgatttt tgtccgcttg ttcctcgcaa taaatgtggg
     5641 ggaacggaag aagaaataca tggataattc ttaccagtag aggttttaag tgtaaaacgc
     5701 tgagcagaaa caacgtaagg attccagccg cctagaggta cgactttaaa aagcccgttt
     5761 tcttggattt gtgtcagcat gaatccgact tcatccatat gtgctgcgac cattacacgg
     5821 ggtgcatccg cttcttcatg atggcgcaga ccaaaaatgc cgcccaaacc atctaactgt
     5881 aactcatcta ccagcggttc catatgactt tccatataag cgcgaacatc ttgttcaaaa
     5941 ccgcttgttc cttgtaattc tgttaactct ttgattcgtt gaaatgtttt ttcttccata
     6001 tgctattcgt ctaaatttca gactaaactc ctctcagact tatattgatc tgcggataaa
     6061 atatgatttt ttctgcagac cgatcgtatt tattatagct tattttttct ggtagcggtt
     6121 atttatttca aagaaaatat gagagtgtgg taaaataagc acaaggagta acaagtgaga
     6181 agaaggaggt atgtccacat gtatgaagaa agcgaactaa gctattttaa aggcgggtta
     6241 gcagttggaa tcggtctagg attattgagc ggggtagctt caacgatttg gtatcatcgc
     6301 aaaaaagtat tgtcagcaga ccaagtactt gaaaatatca agtcatcttt tctaaaagaa
     6361 ggtgcaatcg aaggttcttg gatctctttt gaaaaggaac ctattaggaa attcgccgtt
     6421 cggtcgagtg cttatcgggg aggtctttct cgaatagaag atggcgaaac tgtttattat
     6481 gaatttttag cagatgctca tacaggaacc gtattagaaa tttcaagaaa aaaaggtgca
     6541 acagattaat acgtcaaaaa agtgagagaa cgaatacgca cccactattc aaaagagagg
     6601 tatcatgtcc ttcttttgta gtgggtggtt ttttagatag agctttccaa acatatattc
     6661 cagtattttc tttttaaata aatgatattt agcgggaaaa actagcattt cctttgatta
     6721 tgaggtattt ttaatttagc ttttgttttc tatagttaag gagagtgaaa aatggaacaa
     6781 caagaaaaaa ttgctgttct gattccttgt tacaatgagg aagcaacaat ttcaacagtc
     6841 attgcagact ttaaacgaga attaccagaa gcagatattt atgtctatga taataattcg
     6901 accgatcgaa catacgaatt ggccgttcaa ggtggagcaa tcgtaaaaaa agaacctcgt
     6961 caaggaaaag gaaatgtgat ccgccaaatg ttctttgata tcgatgccga ctattattta
     7021 atggtagatg gcgatgatac ttatcctgca gaagctgtac atggtttgct cgagaaatta
     7081 cgttctggtg aagcagatat ggtcattggt gatcgcctat caaatggaac ctactttgat
     7141 gagaataaga gacctttcca cgattttgga aataaccttg ttagaaatac aattactaga
     7201 atgtataaaa caaaaattct tgatgtcatg accggctatc gaggattcaa tcgtatcttt
     7261 gtcaaaagtt ttcctattat gagttctggt tttcaaatcg aaacggaatt gacgatccat
     7321 gctttagata aaaaattcaa gttcgttgaa atcccaatcg attatcgtga tcgtcctgaa
     7381 ggcagcgaat caaaattgaa tacattctct gacggattca aagtgatcat gatgattatt
     7441 aaaatgttta aagattataa gcctttgctt ttctttggga tttggaccat cttcttcttt
     7501 ttatttggat tggtcacggg gattcctgtg attcgcgaat atatgctgac aagctttatt
     7561 acacgcatcc cttctgcgat tttaagtact ggattgatga ctttagcact tctttcatta
     7621 gtaacagggt tgattttgga tacagtcgtc acgaacgcta agaaagaata tgaattgaat
     7681 ctatatcatg tttatcatga ataccacagt agaaagtaaa aaaaggatca agactgcaaa
     7741 aataatttgc aattcttgat cctcttttta attaattatt tcgccaaacg atagatggca
     7801 tctgcataga ttgctgttgc tctgaacaag tcatcaagac tcataaattc gtttgcttga
     7861 tgcattgtat cagtatatcc tgggaacatc gctccataag caacgccacg tttcaacaaa
     7921 cgtccgtaag ttccgccacc gatgatttgt tcgtagcctt tttctccagt gtggtcttca
     7981 tagacttgca ataatgtagc caccaatgga tcatccattg gtacgtaatg tggttccatt
     8041 acgcgagcac ctcgagtaac tgttgcgcct tcttgcccca ctgtttgttc gatgtcactt
     8101 tgcaatccgt ctactgttac accttttgga aaacggaaat tcatattgat gaagttcgct
     8161 tcctctgtac cattttcttc aaaagcaaat agtccagcat tcattgtcaa tttgcccatt
     8221 ttttcatctt cataagcgac tcctagtttt tctccataaa aatcttcatg gacataagca
     8281 gctgctacat ggataaattg tttagcagat cctaggaatt catagttatc taagaacaat
     8341 gccaagaaag aacctgcatt gataccagat tgtgggcttg caccatgtgc gcctttacca
     8401 actaattcga tttttacata cgtattgtcc gcttcaatcg ttccgcttac tggatttgct
     8461 tcgatgaatt ggtaaaatgc ttcttccatc gcgatagctg catctgcact tggtacttga
     8521 agtgctgcag ttgctgttcc tggcaccata ttttcgcgaa gtccggaaac aaaagatttc
     8581 aagacataat ctcctgcatt gtcgcctttg aagcgtaaag ctaaagaaac atttcctttt
     8641 tcaccattga tgattgggaa ttcagcatct ggtgagaaac caaaatctgg ttcttcttca
     8701 tgttcgaaat aataagccat atcgccccag ccgctttctt catcgctgcc gactacgaag
     8761 cgaacttttt ttgatagttt caagtcaagt tctttaatga tcttcatcgc atagtaagct
     8821 gccatacttg gacctttgtc atcgcttgat ccgcgtgcaa agatctttcc atccttgatt
     8881 actggttcat aaggatctgt gtcccagcca tctcctgcag gaacgacatc catgtgtcca
     8941 aatatcccta gcgtttcgtc tccttcgcca agatcaatat gccctgcata gttatcgaca
     9001 ttttttactt taaatccatc acgttcgcca taagctaaca tatgtttcaa tgcgtcgcga
     9061 ggtccaggtc caaatggtgc atctggtgtt acttttgagt catcgcgctc gctgttgaca
     9121 cgtaaaagat ttttcaagtc ttcaagcaag tcttctttac gtgcttctac ttctttttgc
     9181 caatcaattg tcataaagtt aatcctccac ttatttaagt cttcgtgttc atgataccac
     9241 aaattacaga aaaaaatagt tcaaaaaatc tgacaattcc ttttcttatc tgtttttctc
     9301 taaaaaaaat atacgctata atgagtaaaa ggaggttttt agatgctgat aaacgaacaa
     9361 ctagtgaaaa gagtcatcac acctcgtcca aaaatttcac ataaaggaac atttggacgt
     9421 atcgttttga ttggcggcaa tgaacaatat ggcggagcaa tcattatgag tgctttagct
     9481 gccgtacaca gcggagcggg tctcacgact gtagtaactg atgaaaaaaa tcggagtgct
     9541 cttcacagtc atctgccaga agcaatgatt gtagtttgga cggatactga ccagtttctt
     9601 tccgtaataa agcaagcaga tgtactcttg attggtcccg gattgggtac ttcttctaag
     9661 agtttggaac gtttcaaaca agtactagaa aaccaatcaa aagaacaatg gctggtgatt
     9721 gatggctctg ctttaagttt acttgctgct aatcatcttt tgctacccta tcccgaacaa
     9781 actgtactta cacctcatca aatggagtgg caacggttga gttctctacc gatacaagaa
     9841 caacgggata aagaaaacaa aaagaagcaa caggaaatcg gtgctgtgct cgttttgaaa
     9901 agtcatgaga caaaaattta tggtcaccag cacttctaca ccaatccttt aggttctgct
     9961 gctatggcaa cgggcggcat gggtgacact ttagctggga tgatcacagg atttttagcc
    10021 cagtttcctc aaaatgacca aacgattgca gcagctattt atcttcatag tttcattgga
    10081 gaccagctgg caaaagaaca atatattgtt ttgccgacac aaatcagcga agcacttccg
    10141 aaatggatga attatttttg taaaaaatca atggaaaacg aggcatgagt catggagaaa
    10201 tttacaggtt atgtgcgaga acctttttat tatgaaacag atcaaatggg gattattcat
    10261 cacagcaatt atatccgctg gtttgaagag gctcgggtag ccttattggt acatctaggg
    10321 tttccctata aaaaaatcga agaatctggg atcatcatcc ccgttctgga agtaaattgc
    10381 agctataaag aaatgatcca ttacggagaa aaagtgagca tccatccaac tatccaaaag
    10441 tatacgggga cacggttgga ttttagctac gaaattgtgg gaactgaaga caaaaaatta
    10501 aaaactactg gcagtagtaa acactgtttt ttactttctg attcgcaacg attagtgaag
    10561 ctgagcaaag tcaatccgga gttagatcag ttattccaag cgtacgcttc tttggatgat
    10621 gtaaattctt aataaagacc gtctaagcat ttttcttgta ttgtgtcgag tagttcgtat
    10681 aatgaaaaga aaaaagcagg tgatccccat gtatgtcgtt aaagtaatgc acggttacat
    10741 cgataaaact ggctgtcgca ctcgggaaaa aaacttagac aatctgctca ttttcaaaga
    10801 caaaaaagaa tccgaagctt ttgctaaaag aatcggcggt cgtgtcaaac cgatccaaga
    10861 ggtacgacca gattgattca atatttttct tttcgaggct ctaataggca tgcctatcag
    10921 ggcttttttt gcagactgtg tttttatgcc cacacgattt gtaagcgatt tatgttataa
    10981 tagattatat ctgcatagaa aggaaggtta aatggttatc aataatttgc aacagcaagc
    11041 ttatgaatct atacgtaaac aaatcattta ttccgagtta gcacctggaa gtaaaatctc
    11101 cgataaagtg ttagaagaac aactagctat caggcgtaca ccgattcgag aagcactgat
    11161 ccaactgcgc aatcaagagc ttgtaaaaac cattccacag tccggcactt atgtttcaaa
    11221 aatcgatatc cgatctgctt cattagcccg ttatacgcgg gaaaagttgg aaaaaccgat
    11281 cctgcaagaa tgcagtgcga aaatgaccaa gcaagatcaa gctaaactag aaaaaatact
    11341 agaacaaacc gatcaggcag taatcgctca aaataaaaaa gctttttttg atttggatac
    11401 tgattttcac cgaacttgtt atgaaattgc tggaaaacga gaaatttggg attggctaga
    11461 aagttatagt acgcacttga accgtttcag atggttacgt ttgacgattt cagaattaga
    11521 ctggggtcga gtattagatg aacatcagac aatgcttcaa tcaatgattg atcataactt
    11581 tgatgaagtc ggatttcttt gcacgatgca tttacatatg attatcgagg agcaagaata
    11641 cgtgattcat aattaccccg attattttga agatattcag gacagaccca tcaaataaga
    11701 ggttttggaa tcagcgtttt gacctgagta ttagagaaag aaacggcaaa atagttcctc
    11761 atattttgcc gttttttttg agctaatacc gagggtcagc tgattgcaca ccgtttattg
    11821 agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa cagccaagta aaaatagctt
    11881 cttatgtttt taacttggct ggacttatta ccgcagatgc aagcttttga acaccgtttt
    11941 ccaagaggtc gtgagcctgc cgttcatctc cgagtcacaa ggaacaattt caaaaaacag
    12001 cttctcatgt ttgttgaata ttttgacttg tgaccgagga gttgacagga gaacaccgtt
    12061 tattcggaaa aaaaggactg tgacacgact tttgtcacag tctctttatt tgtaatctaa
    12121 atcatactcc gtacatcttt tttctatttt ttttatttct tctaagatcc cttggctacg
    12181 aatttcatta tatggccaaa gtaagactgc ctctaataca taaggcgaaa aatcgctttg
    12241 attcatctct tttgaaaaac tcatcgctgc tatctcgctc aatgcacgaa tgtttgattg
    12301 atgtgtataa aaaggcaacc gccacagcac aattttttcg ctttgcgtat aaattccctt
    12361 aacagaagct tcgatgtggt gaaatagttc atgtccaata atcaaagctt gcgctgtttc
    12421 tttagagatc gaagctagtc ctgaacattt taatttttct aacggctccg tcatcaatcg
    12481 aatcccttca tcttctgcat aagttgcaaa agtgattcgt tcgtttattt gctctgattc
    12541 acaggaaacg attggaacag ccatttgttc acaaatttct cctacatcgg caaaagggta
    12601 cttttttttg atgctacatg ccgtttcgat accacaatcg attgattgtt gtataagaat
    12661 tttctgcttg gacaaaggaa tctttttcca aaataaatct cgccgaaact ggtagaatcc
    12721 ccaaatttct tcggggatct gttgtaattc gtttagtaac tcttctaact tatgtttgtt
    12781 tgatatttcc ggcaacgacg atcacttctt cctccggttc catagtagat acttccagat
    12841 ccatcaaaag ctgtagttgt tccagatccg tcgctgtaaa atcagagact cttgctccta
    12901 agcagagata aaattctggt cgggcaatca attcagtttg ataaactgcc atatcttccc
    12961 aaagttgttc gacagctgtc atataattgg ctgcagttgt tttcatacat gaagcatgtc
    13021 cacgttggac cttgataaat cctttttgat cgatgatccg aagacttcca gcattttttg
    13081 aacgattctg ttcgccgcat acataaaaga aaggtgtatt ttccaataag caaacttcat
    13141 ttttatttaa tcgcatatcc tctgcagcaa gctctagtgc ttcttctgtt gtgatctcct
    13201 tggttagatc ggttgctttc acttccgtag aaccagtagc gatagccgtt acttttgaag
    13261 tctgtggatc gatttctacg tggacttcga tcgattctgg tgtagcgcct gattcaatcg
    13321 ctttattcat cgcttcattt ttcaacgatc gtatatcttc tttacttggc gaagggatga
    13381 tacgttctac aacatctcgg accattgcaa gtgctacacc aatagaagaa atcacttcgg
    13441 cgttttcagg aatcgaatat ttcacaccca ttttattact aaagtaagta atcaatgagg
    13501 cagcgccgcc ccctacacct acaagagaaa tctgatcttt ctccaagtga tacttgtctg
    13561 ccaattctaa aatcaccggt tcaatcttag cataggattt ttccatgatc tgttctgcga
    13621 tgtcttcaac cgtcgtatga caataatcag ctaatgcttg gatggctttt cgtgcagatg
    13681 ggacatttcc ataagaaaaa tgttcttctt gcactaatcc aaggacattg gctgcacatg
    13741 tatttgtaat agtcacttct tctccgtctt ccatcactac cttgacgtaa tcagcagggt
    13801 ctccttcttt aggagagaaa aattcaacct taggaccttt gattttttct gtctccgtaa
    13861 atactgcata atctagaccg gcaatatgtg cagaacgtgg accgacatcg atgattcctg
    13921 actgattagc ccgtaccatc gaaccgcctg ccacacctag aacccgaaca tctaatgaag
    13981 agatatatgt tgggtggcca cctacaattg aataatcgat tgctggacgt ccatttttaa
    14041 tgaccccgat attggttgtt gttccgccta cttcaaaata aacaccatta gacgctctca
    14101 agtacatcaa agagcccatg actgatgctg ctggaccgga aagcatggtt aaaacaggtc
    14161 gttttttcat ttcattgatt tccattactc cgccgtctcc gcgcataatc atcaaagaaa
    14221 cattgacccc tgcttctcgt acggaatctt ctgtagaggt tgccgtatct agcatttttg
    14281 gtaaaataga tgcattgatt gctgctgtcc gagttcttct tgtcaatcca tataattttg
    14341 tgatgtcaga agccatcgtt gtagggatcg attttaccga tgctgcttca taaacaactc
    14401 gttctggttc cccattatct actccaaatg ccatagaaga aaccaagacc tccgctcttt
    14461 ctctttctaa agaagaaatc gtttctgaaa cacggtctac atttaagtgt ttgacaggta
    14521 agaatgcatt gacaatctct atttttttct tattccctaa atcgatatcg ttcaatctag
    14581 tttgtcgttt tgctaaaaaa ccttctaatc caccttttgc catcccgatt acgccgactt
    14641 ttgctacgtc tccttcaatc agtgcattag tcgcttgtgt ggtagaatgt gcaacaaaca
    14701 caacatcttc tggactgata ttattttcca gcaaacaatt ttgaaaagac tgaacgacac
    14761 ctgcggctac tccacggaca tcatcgtggg ttgttttcac tgaagatttt ccgatgattt
    14821 cgtgcgtcgc attatcgatt gcgactgctt tcgtatgtgt accgccaaca tcaattccca
    14881 ttcgcacaaa acgtttcatt ttcttttcct ccattcatct aatcgttttt atcgacgttt
    14941 tgtttactag gcgttcccgt acaataagaa cgtgatcagg cataagatcg ctccgatgat
    15001 ccaaccagtt gggatggatt tcttcatata ttctcgagaa gttacttttg tataacctaa
    15061 tccccaagca acccaagatt gtgtaatatc taaatgctga ggagcaatcg ttgtgattgc
    15121 aaataacggg aacaaaaagg ctggcgacat cgttggatta acagctaata cgacagctaa
    15181 aatagctgag cctgaaccga ctagattcat tggaccgcgg aaaaatccta gtggtgtcaa
    15241 tatagcaaat acgattgcca agactaatgg tgattgcggt atccaatctc caaaaatagc
    15301 tgaaaaataa ggagaagcat acgcagctga attattgaac atcgctaagg tcatcaagaa
    15361 accaaccatt ggcgcaacat ctactgcgcc atctgtaaat aatttcgcaa actgacgaca
    15421 gatttcttta taacctccgc gcaattttcc agtaaacagc aatgcccaga tacctgctag
    15481 aataaaacct aagatgattg gaagatctaa aagaacgacc cctaaaactg gtaataatac
    15541 ggctaaccat gagatcattg gtgcgtcgcc accttcagct ggtacgtttg ctgccattgc
    15601 atagcgtttc tttttgttca ttgatatatt agcgaccgtt agtaccacaa ctaagcttac
    15661 aatcatccca atcataccaa tttggaaata gtcattataa gtatagcttt cagccgctgg
    15721 attgaaacca gcataaatcg tttgatattg tttgaaattg acgatattag caaagattcc
    15781 agccataatc gagcccataa atgagaacag tgcaaccggt actgaaatac ctaatgacat
    15841 caagattggc aacacaataa ctgcaataga aatcactggt ccgattcctg tcatcgacat
    15901 gaatataaca gcggttacaa tacatagtaa gctcatcgtg attcttggct tgtcccctcc
    15961 aagttcaact acctttcgaa tcagggtagc tgcgattccg ctatctacta gaacacgccc
    16021 gaaaaaggca ccaaagaaga catttaccaa gatcgatttg gcgtactcag ctggccccgt
    16081 cgtatagata tgcgtcaaca catcaattac tccttgccct tccatcgccg ggtttggtga
    16141 aaacgtgttt ccgactagcg ctaatcccat ccatacagta gccatgatgg caaagccgac
    16201 catcaagttg tagcctttta cacagtacca gaccatgaaa aagaatgaga taactaacaa
    16261 aataccaata ataatatcac tcatgttatt cctccgtcca ttttgttttt ctgataagca
    16321 aactaatata tcagtcaaac tcattctaac ttatgtagtt agcgctttca ttaaagggtg
    16381 accataaatg ttcagttttc aattaaaaaa ccttaattat gctattttag acttatacgt
    16441 tattgtcctg tttcatttga tggaggattg taaataaaaa aagaggaaag aacataactt
    16501 ttcgttatct tctttcctct ttataattgt ttagcttatt tagccaaagc ttcttttgct
    16561 tggtcagcca atgcagtaaa tgctgctgca tcgttaacag ccaagtcagc taacatttta
    16621 cggttgatgt cgatttcagc aactttcaaa ccgtgcatta attttgagta gctcaagcca
    16681 ttcatacgag ctgctgcgtt gatacgagca atccacaatt tacggaagtc gcgtttcttt
    16741 tgacgacgat cgcgatatgc gtagttgtat gaattcatta cttgttcttt tgctgtttta
    16801 aataaagtgt gttttgaacc gtagtaacct tttgctaatt ttagcactct tttacgacgt
    16861 ttacgagtta ctgttccacc tttaacacgt gccatgtata attcctccta ataaaaataa
    16921 atcttcagat tctgttattt gtacttctta tttcattttt gctagttgtt ggcgaatacg
    16981 tttgaagtcg ccactagaaa ccatgcttgc tttacgcaat tgacggcgtt gtttttttgt
    17041 tttgccgtgg aaacggtgac ttgtaaacgc gcggaagcgt ttcaatccgc ctttaccagt
    17101 acgtttgaaa cgttttgctg atccgcggtg tgttttttgt tttggcatga ctatatttcc
    17161 tcctcaaaat ctttcgttat ccgactatcg gctgtccaac agccagttag tcattcaaat
    17221 tacttactgt cgtttttcgg tgccagcgtc aagaacatgc tgcgtccgtc cattttcgct
    17281 ttttgttcca ctgttgcaat atccgcagtt tcttcagcta agcgattcaa gactttttga
    17341 ccaatttctt tatgggtaat ggcacggcct ttgaaacgga tagaagcttt cactttatcg
    17401 cccttttcca agaacttacg tgcattacga agttttgtat tgaagtcatt caagtcgatc
    17461 gtaggactta agcgaacttc ttttacattg atcacttttt gttttttacg cgcttcgcgg
    17521 tctttctttt gttgttcgaa acggtattta ccgtagtcca tgattcgcgc aactggtggt
    17581 ttcgcaccag gggcaactaa cacaagatcc aagtttgctt gttctgcaat attcaatgct
    17641 tctgcttttg tcttaactcc taattgctca ccatctgaac cgatcaatcg taactctcgt
    17701 gcacgaatgc cgtcgttaac catcatatcc tttgctatgg tcattcacct ccaaatatta
    17761 tgagagaaag aaaagcttac cgaataaacg gcaataaaaa aacgagttct cttacagaac
    17821 ccgcgcgtac ttcacctaat agaaccagtg aaaactatct agcccagtga cattatcaac
    17881 taaggcgaga agcgggtgct tctgctttta tttctcaacc atcatagtat agcaaaaact
    17941 aatctttttg tcaatgtttt tcatacagtt tgtcttcctt ttctgaaaat gattagatta
    18001 gcagactttc tagcagtgat tcccgcttct ttgctatact aaagaaaaac gaggtgaacc
    18061 cacatgaaaa aaatcgaacc tattccacct tctcttcctc ttcttacaaa gggagagttt
    18121 tatttagaag atgaggagat cttctctggt cttctgatca acgggggtga ccaaagctat
    18181 ctcgatgcca aaaatatggt attaagagag agtcatatcc aaaaacttac gatgcaaaaa
    18241 acagttttac gtcattttga atgcagtaat gtcatttttg aaaaatgtga tttttccaat
    18301 ctggagtgga ttggtggcag tttccaccaa gtcgtgtttc atcaatgtaa attaacaggt
    18361 acgaattttg ccgaaagtta tcttcgagac tgtactttca ctgattgtat tgcttcgatg
    18421 gcttccttta gtagtacaaa cttgaaagct gtctccttcg atcattgtca attagaagac
    18481 agcgaatttt atgaggtcac ttggaaaaac ctttttttgt ctgacaatca gttgaccggt
    18541 tcgaactggt ttcgaacttc tctgaaggga ttagacttta cgactaattt cttcacagca
    18601 atcgctttat ctcaagattc tttggcggga ctaatcgtca accaagagca agcacttgtg
    18661 atcgcccaag cccttggttt gcagatcaaa gactagatca acgaatccgc cgccacagtg
    18721 caataagcat ccaaatagat aagaccaaac tgatgatcaa ggcgattccc catccgaaaa
    18781 cggaatgggt aaagggtaac tggagattca ttccgaagaa tcctgtcaca atagttggaa
    18841 cggtcaagat cagcgaccag accgtcaaaa atttcatcgt gtcattcaag ttgttattca
    18901 gcaaattatt ataagtgcca gagagctggt ctaaaatctg cgaagctaag ttcgtcattt
    18961 caactgcttg tcgtgcttcg atcagcgcat cgtccaactc ttctttttcc ttttctgaca
    19021 acttgcgata aatagccaat gccttgattt gttctaaaag tactacattt tgttttgttc
    19081 ctgtaactaa gtaaaccaaa ccgatctcta aatctgaaag ttgcagcaga ttcttgttcg
    19141 ttgttttttc tcttaatttc agattaagcc gctgacgttc actattcact tcctcgatta
    19201 aaggaaaaaa ggaatcagaa atcaaaaaca atgtacgaaa caataaactg tatacactca
    19261 tgtctggcat tcgttccaaa agcgatcgaa ttagtcgaat cacgtactcg gtattcggcg
    19321 tggtaaatgt aaacaaaccg tcttctttta aaataaaagc gataggacta gtttcataat
    19381 gattatcgac tttttgctgg tgcggaacat ttgatacaag cagtaacgct tcatcaaaag
    19441 aatcgtactc tacgcgcgcc cgttcatttt tatctagaga ataagtcagc atctcgtcac
    19501 taatgccata ttcttctttt aagtttttaa cagctagcac atcgttttgc tgtacattga
    19561 tccaaaaatt acggtcttga ttaaaatagt gtttttcccg catactctat cctcctgaca
    19621 tttgtttcca gtataaagaa aaaagtcgct caaataaagg tttacccttt atttgagcaa
    19681 ctgtaaatca aatggctact ttggtttggc gaatctctgc taatttttct tcgatcattt
    19741 tcaagacttc ttcttttgct tgaggatctt ccacaaaatt atagcggtct ccatcgattt
    19801 ggatttttgg actttctttg tattcttcat accattgatc gtaacgtctg ttcaattctt
    19861 tgtaataatc gtatagactt ggatcaaagg acaattgttc atacgaacga ccacgttttt
    19921 cgatacgctc caacatcgta tcaaaagata cacgaatatg aactaataaa tccggatgtt
    19981 ttttatgggc agcataggga agttcttcca tcatgttttc cagcagttca tcatacacac
    20041 gaacttctgt ttctgttgct cttcctagat ctgcatttag atggaaaagc agtgaatctt
    20101 cataaatcga acggtccaat acattattgt catctttcat tgcttgtttt atgcttgcaa
    20161 aacgtttatt caaaaaataa atttgcagca aaaaagcata ctgttcaggg tttgcataaa
    20221 ataagggcaa gacttcatta tcatcaatcg attcataaaa tgcctgacta tcaaagtgtt
    20281 cagccatcat ttctgttaaa cttgattttc cggcaccaat tgtacctgct aaaacgatca
    20341 cgctcattta cctccagctc acttcatcac taaatattgt gtttaactcc aaccttttta
    20401 aatatacccc caatatctag tagtgtcaat tgtatttttt tctagtcaaa gaagaaaaaa
    20461 agtcaaaaaa ttcttaagaa aaaagtgttt tttctttaga aaacgtattc ttagttttac
    20521 ttctgagatg gttttagtaa aataaaggag gaatgttttt tgagaccctt agtggacaaa
    20581 aaaattcgtg aagcagcgca actcgttttg ggcgctttat tttttgctta aaaatcactt
    20641 ttacgtgaga aggagttaga ttatggcaaa ctatggccaa ggagaatcaa gcggaaagat
    20701 catattgatg ggcgagcacg cggttgttta tggagaacca gcgattgcct ttcctttcta
    20761 tgcaacaaaa gtcaccgcat tccttgaaga gctggatgca atggacgatc aactggtttc
    20821 ttcctactat tcaggaaatt tagccgaagc tcctcatgca ttaaaaaata tcaaaaaatt
    20881 attcattcac ttaaaaaaac agcatgacat ccaaaaaaac ttgcaactga ccattgaaag
    20941 cacgattcct gctgaacgtg gaatgggatc aagcgctgca gtcgccacag cagtcactcg
    21001 tgctttttat gattacttag catttccttt gtctcgtgaa atactattag aaaatgtcca
    21061 gctttcggaa aaaatcgccc acggtaatcc tagtggaatc gatgcagccg ctactagcag
    21121 cttgcagccg atttatttta caaaagggca tcctttcgac tacttttctt tgaacatcga
    21181 tgcttttttg attgtcgctg atacaggaat caaaggacaa acaagagaag ccgtcaaaga
    21241 tgtcgctcac ctctttgaaa cccagcctca tgaaactgga caaatgatcc aaaaattagg
    21301 atacttgacg aagcaagcaa aacaagcaat cattgaaaat tcaccagaaa cgttggcaca
    21361 gacaatggat gaatcacaat cacttctgga aaagctgaca atcagcaatg attttcttaa
    21421 tctattgatc caaacagcaa aagataccgg ggcattgggt gccaaattaa ctggcggtgg
    21481 acgcggtggc tgtatgattg cattagcaca aacaaaaaca aaagcccaag aaatcagtca
    21541 agcacttgaa gatgcgggtg ctgctgaaac ttggatacaa ggattaggag tacataccta
    21601 tgtttaaagg caaagcacgc gcatatacca acattgctct aatcaaatat tggggtaaga
    21661 aaaatgaaga actcatcctt ccaatgaaca acagcctttc gttaacattg gatgcatttt
    21721 atacagagac agaagtcatc ttttctgata gctatatggt agatgaattt tatctagatg
    21781 gcaccttgca agacgaaaaa gcaacaaaaa aagtcagtca gtttcttgac ctttttcgta
    21841 aagaagccgg cctctcacta aaagcttcag taatcagtca gaatttcgtt cctaccgcag
    21901 ctggattagc ctcctctgcc agcgggctag ctgctttagc aggcgcttgc aatactgctc
    21961 ttaagcttgg attagatgat ctctctcttt caagatttgc tcgacgcggg tctggttcag
    22021 cttgccgaag tattttcggt ggtttcgtcg aatgggaaaa aggccatgac gacttaagtt
    22081 cttacgctaa gccagtccct tccgattctt tcgaagacga tttagcaatg gttttcgttt
    22141 tgatcaacga ccagaaaaaa gaagtgtcca gcagaaatgg gatgcgtcgg acagtcgaaa
    22201 catccagttt ttatcaaggc tggttagatt ccgttgaagg ggatctatat caattgaaac
    22261 aagcaatcaa aacaaaagat ttccaacttc tcggagaaac gatggaaaga aacggactaa
    22321 aaatgcatgg aacgacttta gctgcccagc ctccattcac ttactggtct cctgacagct
    22381 taaaagcgat ggatgcggtg cgacaattga gaaagcaagg cattccatgc tactttacga
    22441 tggatgctgg ccctaatgtc aaagttttag tggaaaacag ccatttatct gaggtacaag
    22501 aaacatttac caaattattt agcaaagaac aagtaattac tgcacatgct ggtccaggaa
    22561 ttgcgattat tgaataaagg gagattttta tgattgaagt atctgcacca ggcaaactct
    22621 atattgccgg agaatatgca gttgttgaaa caggccatcc agcagttatc gctgcagtcg
    22681 atcaattcgt gacagtcact gtagaatctg cacgaaaagt cggaagtatc caatctgcac
    22741 aatatagtgg gatgcctgta cgttggacaa gacgcaacgg agaattagtt ttagatattc
    22801 gggaaaatcc ttttcattat atccttgctg ctattcgctt gactgaaaag tatgcacaag
    22861 aaaaaaacat tcttttatcc ttttatgatc tgaaagtaac aagtgagtta gacagttcaa
    22921 atggccggaa atatggattg ggttctagcg gtgccgtgac cgttgcaacg gtcaaagcct
    22981 tgaatgtttt ttacgcgttg aatttatctc agttggagat tttcaagatt gcagcactag
    23041 ccaatttagc agttcaagat aatggttcct gcggcgacat cgctgctagc tgttatggtg
    23101 gctggatcgc tttctcaacc ttcgaccatc cttggctcca agaacaagaa actcagcatt
    23161 ctatcagtga gttacttgcc ctggattggc caggtctatc cattgagcca ttgattgctc
    23221 ctgaagattt acgtttattg attggttgga cgggtagccc tgcctctact tctgatttgg
    23281 tcgatcaagt tcaccgttcg agagaagata aaatggtggc ttatcagctt ttcttaaaaa
    23341 atagtacaga atgtgtcaac gaaatgatca aagggtttaa agaaaataat gtaacgttga
    23401 tccaacagat gattcgaaaa aaccgacaat tactgcatga tttatctgca atcactgggg
    23461 tcgtcatcga aacgcctgct ttgaacaaat tgtgtaattt agctgaacag tatgaaggag
    23521 ccgcaaaatc ttctggtgca ggtgggggcg attgcggaat cgtaattgtt gaccagaaat
    23581 ctggcattct tcctttaatg agtgcatggg aaaaagcaga aatcactcca ctgccgttac
    23641 atgtctatag cgatcaaaga aaggaaaacc gatgaatcga aaagacgaac atgtttcact
    23701 agctaaagcc tttcatgata aacaaaaaaa cgaatttgac ttcgtacgga tcatccataa
    23761 ccctttgccg caaatagcag tttctgatgt cgatctaagt acacaggcag ttgggtttac
    23821 actaagcagc ccattttata tcaatgcgat gactggcgga agcgaaaaaa caaaaaaaat
    23881 caaccaagat cttgcaatcg tcgcaagaga agcagatttg atgattgcca ctggttcagt
    23941 cagcgctgcc ttgaaagtcc cttctttagc cgatacttat acgattatgc gacaagaata
    24001 tcctcacggg aaaatcattg ccaacatcgg tgcagggact tctgtcgaaa gagccaaaga
    24061 agctatccga ttgtttcacg cagatgcctt acagattcat ttgaatgcac ctcaagagtt
    24121 ggttatgcct gagggagacc gtgattttac caattggaaa gtacttattc aagaaactca
    24181 aacagccatt gacgtccccc tcatcgtaaa agaagtcggt tttggcatga caagagaaac
    24241 gctcaatgat ctagctgctt taggagttca tacagttgat atcagcggtc gaagcggaac
    24301 aagcttcacc cagatcgaaa acgcccgtcg ctccaagcgg gaactgagtt atttagctga
    24361 ctgggggcaa tcgacagtct cttcattgct tgaagcaaat gaagcagaca cttctatgga
    24421 aatcctagcc tccggaggta tccgcaatgc ctatgacata tttaaagccc tttgtctcgg
    24481 cgcaaatgcc gtgggtactt caggaaccgt actgacgcat ctgatgaatc acggcgtaga
    24541 agaaactatt atactgatga agcaatggca agaagagctt cgtctgcttt atactatggt
    24601 cggcgcaacg aataccgcgg ccctacacca acaatctctg atcttttccg gaccagtgaa
    24661 ggattggtgc gaagcaagag ggatcgactt agtgaagtat ggaagacgca cagaaaagag
    24721 cgtgggacaa aagtaaagaa cacttttgtc tcacgctcta aacacgtaca aacggcggaa
    24781 gtaaaagcaa ctccttcgga aataagctga aattcacaaa aatttgaaaa gcaattttcg
    24841 tgaattcctt cttatttctt ggagttaaat gcttctgtcc cagcctcttt tttcgaataa
    24901 atgatgttta atcagctgtc cttcggtatt cgttcaatcc gctgatttca caacctctcc
    24961 actaaacagt gtactaaagc tcttttaagg ttgttttccg atataggcaa gtatgcctcc
    25021 gtccacatac aagacatggc cgttaacgaa atcagatgcg ctagaagcca agaagacaga
    25081 tggtccagcc aaatcgattg gctcgcccca gcgtgctgct ggcgtacgtc caacgataaa
    25141 ttgatcgaat ggatgacgtt caccgttttc ttgtgtttcg cgtaatggag cagtttgcgg
    25201 tgtagcaata taacctggtc caattccatt acattggata ttgtattgac cgtattcaga
    25261 agcgatgttt ttcgtcagca tttttaatcc gccttttgcc gcagcataag cactgaccgt
    25321 ttcgcgtcct aattcactca tcatggagca gatattgatg attttaccgc cgcctttttc
    25381 gatcatatcc ggaataactg cttttgaaac gatgaatggg gcatttaaat ccacgtcgat
    25441 tacttggcgg aagtctgccg catccatttc gatcatcggt gtccgtttga tgatccctgc
    25501 attgttgact agaatatcaa ctgaacctac atcttctttg atctgttgaa ccatcgcttg
    25561 aaccgcttct tcatcggtca catcgcagac ataacctttt gcatcgatgc ctgcttcttt
    25621 ataatgcgct ataccttcat caactgattc ttgattcaag ttattaaata cgatttttgc
    25681 tcctgctgta gctaaagagc gtgcaatctc aaatccaata ccatagacag caccggtgac
    25741 taatgctact tttccatcta aacgaaacat atccatacta aattccatca ttttctctcc
    25801 cttatgctta ttttaattga tccatttgca ccatatccat gtctgtatag gtgatatttt
    25861 ctccgcacat tgcccagatg aatgagtaat tgcttgtacc tacacctgaa tggatcgacc
    25921 agcttggcga aataatggct tgttcattgt ctacaactaa atgttttgtt tcatctggtt
    25981 tgcccatcat gtggaacaca cgtgtatctt cattggcata atcaaagtag acataagctt
    26041 ccatacgacg ttcatgtgta tgacaaggca tcgtattcca agcgcttcct ggttctaaaa
    26101 tcgtataacc catttgtaat tgacagcttt cgcatacatt tggatggatg tattggtaga
    26161 ttttacgttc attcagtgtc aggccttcac cagtctccat tggttttact tggtcgatac
    26221 tgatttttac atttggatat ttatgatgag ctggaacaga actaatatag aatttagctg
    26281 ggttttcagc atcttgagaa gaaaatacaa catgtcgtgt ttcttttccg atatagtatc
    26341 cgtcttgttt tttcatttct tctttttgcc catcgatctc gatgaatcct ggtccaccga
    26401 tattgatcac acctagttca cgacgctcta agaagtaatc aacacctagt tctttgtcta
    26461 atgtgatttc taattctttt tcagtaggtg tcactccacc aaagatcatt cgatcgttgt
    26521 gcgtataagt caaactgatt ttccctggtt caaatacctt ttctactaaa aattcttttc
    26581 tcaattgttc cgttgaataa tgacggatat cttctggact gtgtgtataa cgtgtttcca
    26641 tttcttgcat tttcttctac tccttctatc gaatgatttt tccagagccg catgctaaaa
    26701 attgtgcgac ttcttcttga ctaaattggt tgcagtcccc atggatcgta tgcttcaagg
    26761 cagaagctgc cgtagcaaaa tcaatgattt cttgcggaga catattggat aaaatcccat
    26821 ggagcacgcc acctgcaaaa gcatctccgc cacctactcg atccacgatt ggggtgattc
    26881 tatggacttg ggattccaca tattcatggt tcatccacaa tgtccccttc aattcatttt
    26941 cacttgctga atgaacggta cgcttggtcg aataaaacac ctgaatattc gggaactttt
    27001 cctgcatttt ttgataataa taaattagag gttgttcttc tgcttctggc gctttctcga
    27061 tacctaacaa ataaagagca tccatttttc cagctgagca gatatctact aaaggtaaaa
    27121 gctgatggat tacttcgctg gcttcttgct gtgtccataa ttttccacga tagttgatat
    27181 cgaagctgat caggcagcca gctttttttg cagcttctat cacctgtttc gtcatttttt
    27241 gccatttttt agacaaagct ggggtgattc ctgaaacatg aaatatatct attccttgga
    27301 ataactcctc tagcgaccat tccaaatgat ccatttcagc aaaacttgat ccggcacgat
    27361 catagatcac tgaggcggcg cgttcgccac taccagtttc catataataa gtacctagtc
    27421 ttgatccacc agataatacc tgtttcgtat ctactccata acattgcaag tgctttttga
    27481 ctgcttttcc caatgcgtta tcaggtactt ttgtagcaaa aatcgcttca tgtccgtaat
    27541 tagctaaaga aattgctacg tttgcttccc ctccgccata atgggcagcg aatcgttcac
    27601 ttgaatcgat acgggttccc tgctctgtgg acaatcgcaa catgatttct ccgagtgtca
    27661 ctacttttcc cataagcagc tacctcttga tttctttcat ttttgctgca tactgttgcg
    27721 cgacttccgt tactttgtca aaatctcctg tagctgcggg tgctaaaagg ttcccaccta
    27781 ctccgactgt tacgacacca gcatcgaacc actctttcat attgtccaga ctcacaccgc
    27841 cagttggcat gatattgact tgaggcatcg gcgccttgaa ggcagaaatg atacttggtc
    27901 cataagcgct tccaggaaat agtttgacga tatctacccc gcttttcagc gctgttttga
    27961 tctcggtaat agtcatacat cctggtaagt aaggaatttg atacagattg cacatctctg
    28021 ccgtttcttg atcaaaggaa gggctaacaa tatactctgc gccagctaag atagccaagc
    28081 gagctgtgac agcgtctaat actgtgcctg cgccaatcac tatttctggt tggtctttgt
    28141 agaatgacaa aagttctttg attacttgat ctgcttgagg gactgtaaaa gtcaactcaa
    28201 taccagttag accacctttg atgattgcgt ggcaagcatt caaagcttct tctttgcttt
    28261 ttccgcgtac caccgcaatc actccggcgt ttttcaatcg agataaaata tcaatttttt
    28321 tcatcttctc cacctatttc ctatttagaa attttatttt ataacttgga aatctatacc
    28381 catattatac ggcagaaaag aaaaattgca aacgcttttt tcattatttt tgaattcttt
    28441 gctcgatttc gctttttgtt tgcttgaggg cactgattat ttgttctttt gtctcatctg
    28501 ttaaacgata tttcggcata cttacactaa atgcaccaat gatttgttcg tttttcacga
    28561 ttgaggctcc tatacaaaaa atatcacgtt cgatttcttc atcgtcaaat gcgatgtggg
    28621 tttcttttac cgttttgatt tcctttttca accgtaatgc attggtgatc gtatattctg
    28681 tgtaagggat caacggatgc ttttcaagat aatcagtgta ctgctcttca ctgaattctg
    28741 ctaatacggc tttgcccatc gcagaattgt ataatggtcg ggtgattccg atttttgaag
    28801 acatgcggat cgtttgatgt tttggatcta gtttgttcac gtacaagatc tcattgttgt
    28861 ccaatacccc taagtggata gtctcatcga tcttttgctg cagctgttct aaaaacggta
    28921 aagtcaactc taccaaatcg atttgttcaa tgtttttatt cgcgtagcga ataagctttg
    28981 cacctaaacg atagtttttc tctgaatttt tatttacgta gccgatcatc aacaaagtat
    29041 ctagtatttt caagactgta gaagaggtca ttcccacacc ctttgcgatc gtctgcaaag
    29101 agacatcttg gttttccgct aaatagtcta gtattttaga ggctttgatc aatacagttc
    29161 cataaggctt tttattttcc atataatatt ctcctatttc tttataagaa aaattatagc
    29221 ggaaatacca tcaatttaaa agagatatcc agatataaac taccatttct ctttacaggc
    29281 aatttaatgt tgaagatccg cttcgttttt acatcaaact ttgatataaa gcaatgattt
    29341 cttctactgt cgtttctctt ggatttcctg gtgtgcatac atctcgcaaa gcatcttctg
    29401 caattgctgg aatatctgct tcttttacac ctaattcaga aatggtttct gggattccaa
    29461 ccgctttgct caaccgatcg atctcaccac aagccgcttc tcttacatct tctaaagaca
    29521 tttcagctgc tcctttaatc cctagaacaa gagcgatctc acggtacttt tctccagtat
    29581 attctttgtt atatttcatg acagttggca aaagtgctgc acacgctacg ccatgaggaa
    29641 tgttgtacca tgcacttaac gggtgagcca tcccgtgtac cagtcctaaa ccgacatttg
    29701 agaagcccat tcccgcaata tactgtccta atgccatttt ttctcgccca ttttgatcgc
    29761 catctacaga agcttccaaa gaatggccga tgatttcgat tgcttttaaa tgcagcatat
    29821 cagtcatttc ccaagcgcct tttgtgataa acccttcaat agcatgcgtc atagcatcca
    29881 tacctgttga tgcagccaat tttttcggca ttcccatcat catatcgctg tcgatgaacg
    29941 cgacgattgg gatatcatga ggatcaacac aaacaaattt tcggtgattt acttcatctg
    30001 tgatgacgta gttgatcgtt acttctgctg ctgttccaga agtcgttggt atagctaata
    30061 taggtaaaca tggatttttc gtatcagcaa caccttctaa actaatgacg tctgaaaact
    30121 ctggattcgt caaaataatc ccaatggctt ttgccgtatc gattggcgaa cctccaccga
    30181 ttgcgatcaa acagtctgcc tctgcctctt taacaaaagc cactccatct tttacatttt
    30241 ggatcgttgg attaggtacg atcccgtcgt ataaagcata agcgattgct gctttatcta
    30301 gaagttctgt tacttttgtt gctataccgt gctcgatcaa tcctttgtca gtaatgacag
    30361 ctgctttgcc gaatcctcgc tgctgaaatt cagggacgat cgactcaatc gctccttttc
    30421 caaaatacga tgtttcattt aaaatcattc gattactcat tttctattcc tcctaatttg
    30481 tattattttt cacaataaaa acgaaagaac cccaagcaac cggttgccca gttgcttgtt
    30541 gctacagttc aaagactggc atcaattccc aactgactgg agaggcatct ggatttgtct
    30601 ccatgatatc ttccatgtac ttccaccatt tttgattgat agatgtattt gccatttttt
    30661 cccataactt ttcatcttcg atcactaagt aaccaaataa caaacttgta ctcggatcaa
    30721 gatagatcga atagtcgatg accccgtgtt cacgaagtgc tgtacgcatt tctggccaaa
    30781 gttcatcatg tctttttttg tattcttctt gtttttctgg gtaaactttc atgcaaaaag
    30841 cttttttcac catgtcagac acctgctttc gtatgaactt cctccaaata acctgctttt
    30901 ggagtgacac caaacgcatc tgctaaatgc cacaggtttt cgtccgtgat tgtttgcaag
    30961 attggtcctt gcgctttgac gtacgtatag acttctgctg ccttttctgc tgtctcgatc
    31021 aaaccaaaga cttcatccat gtcttttcct gacccgtaga ttccatgttg tggccataaa
    31081 accaatcgag tttctttcat tttttcagct gttgcttccc caatctcatt tgttcccgga
    31141 accatccacg gaatgatgga aattccttct ggaaaaacaa ctaagcattc cgtacacatt
    31201 tgccatagcg tacgactaaa ggaacgttcg tctaattcat gcgtaaaact catcgctaat
    31261 agatgactag catggcaatg cataacgatc cgattatctg ggtctacttc taaacgggcg
    31321 atatgactca tgaaatgact tggcaactcg cttgtaggaa ggcattcgtt ttccagcccc
    31381 cacaaaagtt ccagtgtttt gccatcttca gcaactcgaa cgattccaag attctcggca
    31441 ggttcgctcg ctacattttt aaaatatctt cctgaaccag ttacaataaa atagcatcct
    31501 gctaaacggc tggcatcaaa aatcatcgag atgcggcgga tcactttttc aggatccaag
    31561 aacggtgtga tctcttcttc ttttagtaaa taagaaatat tcccgccatt tctttcatcc
    31621 catcccaagc gataaagatt cgacgtcgtt tcgatcattt ctttcacata aggcgcttgt
    31681 aaaatatcta tttttttcaa tatagccaca ctcctatttt cttaattcca acacgtcttt
    31741 ttgatattcc tggacttctt ccagccaatc caaaccaact ggaacttgat ttttgtaaca
    31801 gaaataattc catacatcgg catacgggaa atctttcaat tcttccgtat aagccattct
    31861 tgaagtaaag tcaaaagcca gctccatttc ttccaatcgc tcaactggtt ccagcatagc
    31921 tttcagcagt gctttttgtg tatttctcgt accgatgacc caagcagcga tacggttgat
    31981 cgttgcatca aagaaatcca gaccgatatt tgttttttcc aacaattcgt tacggactaa
    32041 ttctttagcg atttcctgca attcatcatc catcaaaacc acatggtctg aatcccaacg
    32101 cactggacgg gaaacatgca acatgattcc tttgctgaat aaagctaatg aagataattt
    32161 attggaaatc acttctgttg gatgaaaatg tccagcatct agacaaacaa gtttgtcacg
    32221 agtcaacccg taacccatat aaaattcatg ggaaccgact gtataagctt cagcgcctaa
    32281 accaaatagt ttgctttcta ctgcatcttg tacataccgt tcatctaact cttctgtaaa
    32341 aatttcatcg agtgattcca ttaaacgttc ccgtggggta aaacgatcaa tcggattatc
    32401 tttcatccca tcaggtaccc aaaagttatt gatacttgtt tgcccagttt ctttcccaaa
    32461 gtactccgcg attttgcggc ttcttttccc gtgttcgatc cagtattcac gaacttcttt
    32521 atctggatga gccaatgtaa agccgtcttt catcattgga tgcgaaaaga aagtcggatt
    32581 gaagtcaaga ccgatttctt ctttttttgc ccactctacc catttttcaa aatgtttcgg
    32641 ctcaatttca tttagatcga cagattcttc tgtatccaaa tagatagcat gaaggttcaa
    32701 tttgtgtttg cccggtatca acgaataagc cttctccaga tcttgtctaa gctcttcagg
    32761 tgttcgtgcg gctcctggaa aattccctgt cgccatgatt ccaccggtca attcgccatc
    32821 cggtgtcaag aaacctttga catcatctcc ttgccagcaa tgcatagaaa ttttgatgtc
    32881 ctgtatcttt tctagtacag catctgtatc tacaccgata gttgcatatt tttcttttgc
    32941 ttgttcgtaa tttctttcaa tcgtgttcat acgattactc ctttgatttt attcacgtac
    33001 cattccatcg tgttttgctt attttcatct gggtaaaaag tttcacatgg aaacgactgt
    33061 ttgattacat ttcttgcttg ttctatattc gaaaactgac ctgtactgag catttgcatg
    33121 gcaacattcc cgattgctgt ggcttcacta ggtcctgctt ctacaatgat tccggcataa
    33181 tctgctgtca attgattcag tagacgatta ttcgatccgc cccctacgat gtgcagcttt
    33241 tccaatttcg ttccagttaa gtgttctact ttgatcagct cttgtgcgta acaaagagca
    33301 agattatcat acacacaacg agcgatagct cccgatgaga cagggactgg ctgctctgtt
    33361 tcaaaacagt attgctgaat ctcttctatc atatttgggg gattcaaaaa tcgagggtca
    33421 ttgatttcca tcacttgctg cagcggtttt tcttttgcag caagctctgc caattccgca
    33481 taactgtact cgtactcttg cattcgggca acttcctgga tcaaccacat ccccatgatg
    33541 tttttcaaaa aacgcaccgt gttatgggca ccccattcat tcgtataatt ttcagaaaaa
    33601 gcattgcgag aaacattggc gatctttgtt tcaatcccta aaagcgacca tgtcccactg
    33661 ctgatataac cccaacgttc tccttgacct ggcgttccta atacagctga cgccgtatca
    33721 tgacttgcta ctgtgataaa ggtcacatct ggcaaatcat attttgaaaa ttgttctttt
    33781 tgcagatctc ctagtatcgt tcccggctct gtcaatggtg gaaataattc cttcgagaca
    33841 ccgatttttt caagaagttc tatttcccat tcttttgtcc ctgcattcag cagctgcata
    33901 gtagatgcat tagttttctc cgtcaccatg ttaccagtaa agacataccc taaataatca
    33961 ggtatcagta acaatttatg tgcttttgcc aaattttctt tttcttcagc aaacaattgg
    34021 aacaaggtat tgaaaggctg gatctgaatc cctgtctgct catacaactt atctagagaa
    34081 tagatttcag aaaactgttc tactgctcca ttggtacgct tgtcccgata agcaatcgga
    34141 tcatttatcc gatccccttg ttcatcaatc agacagtaat cgactgccca tgtatcgatc
    34201 cccgcaaaaa ggcgatcaat ccctgtctgc tttaattttt caaatccaat caaaatctct
    34261 ttgataagat aatcgatctt ccagcgatcg tgaccgtctt tgttccgaaa gccgtttttg
    34321 aaccggtgta cttcttccaa gatcaaagaa ccttttgcat ccctcgtact tttcattagt
    34381 cgtccgcttg atgcaccgat atcgatggca acaaatgttg gattcatttc tccacctctt
    34441 ttactttatt gttgattagc actttattaa taaagcgtta tcatttattg acaatgctat
    34501 cataatacag aatcttatat tttgcttcac actattttgg tatataattg cattatcttg
    34561 acaagttggg agtgtttaca tggattactg ggaaaaagcc aaccactcat ggtcggaaga
    34621 ctcaaaaaga gtgatcttga cgcctacaca aagaagcagg aatttatttt tttatatcca
    34681 agaaatcggt tacttcaaag caccaatccc ttactatact gaacgagcac atttaccttc
    34741 ttacttgata aagtttactt tgtctggcaa aggttcgctt acatataatg ataatcagca
    34801 acagctaaaa gctggagact tattttttat tgactgccaa gaatatcagc gttatcaaac
    34861 gatcagcgaa gaaccatggg aaatggattg gatccatttg aatggtcaga acgtccaatc
    34921 attttatcag gagttcacaa aagacggcca taacaccttc cattcaacag gagatccatc
    34981 agaaaacaag atccaccatt tgatcacaga attgatccac atgcaacaag gcacgaatgc
    35041 ccgtaccgat ttccagtcct ctgtagctat ccacgagttg ctgaatgaac tgattttgca
    35101 aaagtatcag ctagatttcg aagaagagga tattcccgcc tacgtttaca aactgaagca
    35161 atacttagac gaacatttca aagaaacgat tacacttgat caattagaac aacaattctt
    35221 attgaataaa tatcagctga gcaaagattt ttccaaattt atcggttctc caccaatcga
    35281 ttatttgatc agcaaaaaac tttcttacgc taaagacctt cttcggtata cagaatttac
    35341 ggttcgaatg atttccgaag aaatagggat tgagaatttt gcttacttta gccgcctgtt
    35401 caaagcaaaa acaggactat cgccgagaga ctttcgtaaa ttcagctgat tcatataata
    35461 tgtacgtcat tttttcaaaa tattttgaaa tatatagata aaaataaaat aaccgcagtt
    35521 tctgcttcta tgtaaacaac agcagctact gcggttattt tttagtgatc attctcattg
    35581 ttcatcttca aaatgttcat aatgaaccaa ctgtttctgt gtatcacgtt tcaaaaattc
    35641 tttaggcgtt atgccgaact ctctcttgaa tgctttcaga aaatgcggat aagaagaaaa
    35701 accactttta ctatatacag cactagagct gcggtattct cgcaacaagt gcttggcata
    35761 gatcagcttt ttcttcatta catactgatg aaacgtaaat ccagtgtact ttttaaattc
    35821 tcgtgtgata tggtatttgc tgacaaaaaa ctgcttttcc atttcctcta aagaaagcgg
    35881 atgagataaa ttttttgaga tatattgtaa catttgttcc acacgctcat taggaactac
    35941 agcattctgt tcttgcgcac tctcttcttt ttgtacaact tgattgagaa aaatcaagta
    36001 ttctaccaga gcttgttcat attttatgtc ttcaccgtaa atttttttat caggaggatg
    36061 gtcgataaat gataattttt gcttcaatac ttcctgttct atttgcaata cacgactcct
    36121 cttctctccc attggttgaa aacaagccga tagattagac catggcgtgg aacgactttc
    36181 tagaaaagaa ggggtgacaa agatatagac acgctcgaat acatctgaag attggcggac
    36241 gatacgatgc aaatcattgg aatggatgag cagcacactt cctgctttga tcgtaaattg
    36301 tcgtccgttc aaataaaaag tcgcttctcc ctttaatgta gcatggatct catagaaatc
    36361 atgatgatga tagatcgttt tattttggct gacgacatct ttgacatgat agatttcata
    36421 ctgatcagaa accatcgtat tgacttgttc accgctgatt ttcttttgga gcatgtgccg
    36481 cacctccttt tgttcactta tgcacaattt tattatacca cgttttttgt aatcaaaaag
    36541 agctgctttt atatactctt ataaaaaaga agagacgtgg catcttctag ccccgccccc
    36601 tccattctaa atattagtta accaacgtct tctttacatt attcaagaag acttcttcca
    36661 taacgaatcc catcatcttt tgtttcttca agaaccacat acaggtacgg acgattcttc
    36721 atgatgattt ctttgatttt tgtgtactcg atcacaccgt ctcccaaatt taccggcacg
    36781 attttttcct gttcgacacg aaaatctttc aaatgaaccg cacatatctt ttcaccaaaa
    36841 cgttcaaaag cttcttctac caattggact tgttcttgat aatcactact tgtgatcaaa
    36901 ttggtcggat ctaaaatgat tcctagatag tccgaatcta ctgcttggat cagctgctct
    36961 acccttgcta aagaatacac cggatgattc aaacctggtt ctatccccac catcatttgg
    37021 tatttttctc cagcttttac caagcgacgg ataacggaca cagcttcagc aaatgcttca
    37081 tctgtgaaat tttcttccgt atattggatt tccggcaaaa cacatccagt ttccgaagca
    37141 accatcgacg caccaaagga agaagcatag cgaagatact gctcgaactt tgtcagcaac
    37201 tcttgtcgta tcgttaaatc tggatgaatc atattgatat agcaacttaa aattcctacg
    37261 gtcacttctt gcttttctaa aactcgtttg acataatttc ccatcccaga attgatctca
    37321 tttgcgccag aaggtatttc aggaaacgaa agacctaagg cgagctgcag tgtatgaatc
    37381 ccttgttctt ttgttttttc tgcaagatct tctatcgttt ggacttcgga aagatcatgt
    37441 cctctaattg taatgttcaa cataactatt ctcctaactc agcatattgg gtagccacat
    37501 gacgatttgc gggaagtaag taatcaaaaa caagacgact aagataaccc cataaaaagg
    37561 aagaagcggt ttcagcattg cctctgcttt tacgcctcct acacttgcac cgacatatag
    37621 tcctgtacct actggcggcg tgatggagcc tacacacaga ttcatgattg aaaacaaccc
    37681 gtaatgtaca ggatcaaccc ctacacttgt gacgattggt aaaaatattg gtgtaaaaat
    37741 caagatagca ggcgccacat ccataaacat acccaccagt aataacacaa gattgactaa
    37801 taacagaata ataaatttat tatccgttac gcccataatc aacgaactaa ttgcctgagg
    37861 aataccggta aacgccatgg cgaaagacat catggaagaa gtagccaata agaacatgat
    37921 cgtccctgac attgctactg attgaaacaa catattaggt atatctttta gttttaccgt
    37981 tttatagtaa aacatagcaa tcaacaggga gtaaatgaca gctatcgctg aagcttctac
    38041 tgctgtaaat atccccgtca agataccacc gataatgatg acaatcaata gaaggctagg
    38101 aatcgcctct tttattattt ttccagcatg agccatctgt cctttttcga caacagggta
    38161 tttattcttt ttagcaacga tgaatgctac tagcatgatt cctagcgccc acaaagcgcc
    38221 tacgagataa cctcccataa acaaagcaga gatcgaagca ccaccagcaa caagtgaata
    38281 catgataaaa gcagtacttg gaggaatgac catacctgtc ggagctgacg cgatattgac
    38341 cgctgtcgca aacttccggt catatccttc ttccacttgc tgagggatca acactccgcc
    38401 aatcgcggta gaagcggcaa tggccgagct agagatggaa ccaaataaag cattccctaa
    38461 cacattcgta tgagccaaag cccccggcac gcgcccgacg aacaacatcg caaaatccac
    38521 tagtttctta gctatccctc cattattcat gataatgccc gagagaacga aaaacggaat
    38581 agcgatcagc gaaaaactat tgatacttcc caccattttt tgagctgaac taaaagcaga
    38641 aacatcaaac ggtataacta acaataaagt catcatcgat gagaatgcaa tacttatagc
    38701 aatcggcatc cctaatacaa gcaacacaac aaatacaaaa aataacacta atccggcttc
    38761 tattgccatt ttattcctcc atttttatat tttttcttga attgctggct tgttgcttaa
    38821 tgtatcgatt agattgatca ggctatagat aatgatgagt acccccgata caggtactga
    38881 cagataaaca tatcccatag gaatacttaa agaaggcgag atctgcccca ttgtcaatgt
    38941 gacagcattc catccgccat acaccatgat catcgcagca aataccagaa ataaactttg
    39001 aacaacaact tctaaaattt tacttttttt gccagttaat ttttcactta aaaccgttac
    39061 ggctaaatgt gctttttgtc ccacaacata cgcggaagac aacatggcaa accagacaag
    39121 accgaatcga acaagctctt ccgtgaccgt atttggattt cctaaaaccg ttctggaaaa
    39181 tacttgccat aaaacaacag cgaccatccc aacaagcaaa acagctccca ggatctctaa
    39241 taccttgttt aaaatcattt tcatcacagc tacgctcctt gctcttggat cttttgataa
    39301 aggtctttgt aggtaggatc gtttttgaat atatcatgaa gtggggcaac tgcctctcga
    39361 aacggtgtat tatctacatc attgaattgg actccatgtt cttcttgtgc taaagctttt
    39421 tcttcttcaa ttgcttttgc aaaagcttca atttcaaaaa ccgttgattc tttcgctgct
    39481 tcttcaatgg ctttttcctg ctcatcagtt agctgttctt tgatggcatc attcatgatg
    39541 atgatatctg gcacacgtgt atgctcatca taggaatagt atttagccac accaccatgt
    39601 ccagctgtat acaagacaaa ttcattgttt tctgatccgt tgatcaaatt ggattgaaga
    39661 gacgtgtaca cttcatcact tcccataggt acgggcgaac cgcctaaaag ctctaccatt
    39721 ttgatagcgg tttcgctttg catcacacgg atcttctttc ccttcaaatc ttccggcgta
    39781 tggatcggac cgtctaccat ataaaagctg cgctgaccag aatcataata ggtcaatccg
    39841 gtaaatccta aatttttggt cgattggtag attggctcca ttatttcttc attatccatc
    39901 accttaaaga aatgttcttc atcattgaat agataaggaa ctgaaaaaat cgaataatct
    39961 ttagagaaac tttccaacgc tgaggcactt acttttgtga aatctactgc tcctgactgt
    40021 gtcaattcga tcagttcacg ttctccaccc agttgtccat ctgggaaata ctggatctgt
    40081 acggaaccat ctgttttttc ttttactaaa cgcccaaatt cattcattgc atcaattact
    40141 ggctggctgt tgcttgcata agcaaagcgc aaggtaacta tctcatttac atctgcatct
    40201 gcgacagttg ccacactaga attatttccg cagccgctta gacctagcag acctaggcta
    40261 agcgaacttg ctgcaaacag cagtgatgtt tttttcatat gggtttcctt tcttttaaca
    40321 ataagagacg agcaattctg tcaaacaaag caccgttaac gactgtccat aaggcattgc
    40381 tgtgattccg atattccgat aaaaatctaa actgtccccc attcctgtcc cgattgaaac
    40441 gttttgaact tctccttttt catcgatttg ttcaagcaat ccttggatag cacgataagc
    40501 gacttcttta tattcttgtg gtaaataacg tttatggacc gctttcaaaa taccgtaagc
    40561 aaatcctgca gttgcagaag attctacgta agaagaagga tcatccaaca acgtatgcca
    40621 taaaccgctt tcagactgat aagtttttaa tgcacgcact tgtgcttcaa gtgtttcgat
    40681 caataatttg cgcaatgcat cttctttcga taattctaag atttcaatga tctctggaat
    40741 agcaatcgtc aaccagcagt ttccacgagc ccaaaatgct tcggcatagt tatgatttcc
    40801 ttcaaacgtc cagccgtgat accacaatcc tgttcgttta tccatcaagt atttgatatg
    40861 gatcataaac tggtaacgag cttcttctaa atattctggg cgattcaaca gtttcccgat
    40921 ttttgccaaa ggaagcactg tcatcatcaa tgtatcgtcc cacaactgat tcttattttc
    40981 cggtccatat gtggcatgct gtaacccgtc ttcatttgtc cgaggcatgt cattcatgac
    41041 ccattctgcc cattgttcta aataaggcaa atagcgtgaa tctttcgtgt cttcatacaa
    41101 gtaagccata gtcaatagcg gtgccattgt attgacgttt ttgggcggag cgccttcttc
    41161 catacgatcc tcaaaccatt cattgaccac ttgataagcg cgaggatttt ttgtcatctg
    41221 gtaattttta taaataccat atagaccgac accttgtggc caattccaca ctgtccagct
    41281 tttgtcatct actttcagtc catcaaagtc caacaaaaat tcaccattat cgtctttgat
    41341 cgttgtcagg ttttcaacga gcaggtcaat tttttctttc actaattctt taggtacaaa
    41401 aatctcttct ttaataatca tctttacgcc acctttttaa acgctttcat tttatatcct
    41461 taatatagca gtaataccgt ccactataaa tatcgctttt tgaacaaaaa ctgtctaaaa
    41521 ttgacctaaa tgttaaataa caaaagtaac tttcagttac ttggaaatta aaaaaagaac
    41581 ttggagaaac aacgttttcc aagttctttt tcatctattt tcttatcttt tttctacgct
    41641 ttcttttgct tatccgttgt aagcatcaat gatgatctgt ttcaattcag agatcaatgg
    41701 ttctttcgga ttagctgtcg tacattggtc ttcgtaagct aattcagcca tacggtctac
    41761 ggttgtatct agtgtttctt gtgtgactcc ttgagctttt agactcatgt tgatgccgac
    41821 agattttcct aattcataaa cagccgttgc taaagcttcg actaattcct ctgttgtttc
    41881 gcctttcaga cctaagaatt ttgcgatatc tgcgtaatct gtatctgcac ggaaatagtc
    41941 atatttaggg aacatcgcat gtttttgtgg atcttttgca ttatagcgga taatatgcgg
    42001 taaaagaatc gcatttgtcc gtccatgagg gatcccgtat tccccgccga ttttatgtgc
    42061 gactgagtga caaataccta ggaatgcgtt agcaaacgcc ataccagcca ttgtagaagc
    42121 gttgtgcatt ttttcacgtg attccatatc aggtgttttt actgattttt ctaaatagtc
    42181 aaagactaac ttgattgctt gtaggctcaa tccacgtgta taatctgaag ccatgactga
    42241 aacataggat tcgattgcat gtgttaacac atccatcccg gtatctgctg tgacagatgc
    42301 tggaactgac atgacgaatt gaggatcgac gattgcgaca tctggcgtca atgcataatc
    42361 tgctaacgga tatttcacat gtgtttcact gtcagtgatg accgcaaatg gcgttacttc
    42421 agaaccagtc cctgatgttg tcgggataca tacgaattgt gttttttctg gtttgtcgat
    42481 cttatatgtc cgtttacgga tatccaagaa tttttgtttt gcaccaaaga aagatgtgtc
    42541 tgggtgttca tagaacatcc acattccctt agctgcgtcc attgcagaac cgcctccaag
    42601 agcgattact gtatctggct tgaagtctgc catcagtttc gttcctgcgt atacggtatt
    42661 tgtcgatggg ttcggttcaa catctgagaa gacttcgatc ttcacatcat ttttacgtcg
    42721 tgccaatact tcacgaactg tatcgcagta accgaattgg accataccag ggtcacaaac
    42781 gatcatgaca cgttcaacgt tttccatttt ctctaagtac aatagagagt tcttttcaaa
    42841 gaagattttt ggcggtaatt tgaaccattg catgttattt ctccgtttcg ctacagtttt
    42901 gacattgatt aagtttacag cagagacgtt acgagaaaca gagttcttac cataagaacc
    42961 gcatcccaaa gtcaatgacg gaatcatttc gttatagata tcaccgatgc cgccttctgc
    43021 agatggtgta ttcactaaaa tacgacaagc tttcattcga agaccgaatt tgacatgcaa
    43081 ctcttcgttt tctgaatgga taactgctgt atgtcctaat cctccaagat tcaacatacc
    43141 ttcacacaaa tcaaacgcat ggtccgtgtt gttagctttg atcatggcaa gaactggaga
    43201 aagtttttct cttgatagcg gataatctgg gcctacgcct tccaattcag caatcagcat
    43261 tttcgttcct tttggtacac tgattcctgc caattttgcg atttccatcg cagaatgtcc
    43321 aacgattgat ggattgaccg catattttcc ttcgttcatc actgcatctt ctaatttttg
    43381 caattcatct ggttttacga tataaacttg gtgtgcttgg aattctgctt ttactgctgc
    43441 atagatttct ttatccacga tcacagcttg ttcagaagca caaatcattc cattatcaaa
    43501 tgttttcgaa acgatcaaat cgttcactgc acgtttaatt tttgctgttt tttcaatata
    43561 agctggtacg ttacctggac ctacacctaa agctggtttt cctgtagagt aagcagactt
    43621 gaccattcca gctccacctg tcgctaagac gatcgcgata cctggatggt tcattagcat
    43681 agaagtcgct tcgatcgaag gatgttcgat ccattggata caattttcag gagctccggc
    43741 agcgatagca gcatcacgta ccactcgtgc tgcttctgca gaagattttt gggcacttgg
    43801 atggaaagca aaaacgattg gatttcttgt tttcaacgcg atcattgatt tgaaaatcgt
    43861 tgtggaagtc ggattcgttg ttggagttac tccgcaaacc acaccgactg gttcagcaat
    43921 ttcaatcaac cctttttgga catcttcatt gatcacacca actgttttat catgtttgat
    43981 attatgccaa atatattcgg acgcatacat atttttgatt gctttatctt cgtaaacacc
    44041 gcggcctgtt tcctctacag ccatcttcgc taatggcata tgttgatcca aagctgccat
    44101 tgccatttca tgcacgatat ggtctacttt ttcttgatca aagctttcca tttctttcaa
    44161 tgctgcattt gctttttctg ccaaatcatc aatcattgct tgtacgtcaa ttgtcttcgt
    44221 tttttctttt tctattactt tagccatcat tatcctccta atgggtcatg gttatttgtt
    44281 aaataattca catgtatatc atacaatatt tatttttcta tgtaaacact tttttgtgat
    44341 ttttttcaca gttattttta aatataaaat tgctttattt acaggtgttt gtatagaaaa
    44401 atatataaag ttatttatta ttatttatat gacacacccg attattcaat attgagcaat
    44461 atatcccatg ctcttatctc tttctttttt gaataaaaaa tgaggtagga catctttctg
    44521 ccctacctcg tctttaaata agctttgata cttatttttc ttccttctct tctgtctctt
    44581 ccagcgtttc tgttgtttca gtttttactt caactgtctc agctggttct tctgttgttt
    44641 caacagtagt cgttgcatca tttgttacga cagtccctgg tttgaccgtt ttgattgctg
    44701 cacgatcgta ctcaaggaag atcccttcgc agtctaaaac aactgtccgt ttgtcattgt
    44761 cgatttctga aacaactcca tgtaatccgc cgatcgttac gactgaatca cctggtttca
    44821 tgctatctaa caagttttga cgttcttgct gttgtttctt ttgcgaacgt gacataaaaa
    44881 accacattgc taatagcgcg ataaacatca caagcattgg taatgttccc atttttttca
    44941 cctcactaat tctgtatcta caaaaaacac tttagcagaa aaatgctgat aacactagtt
    45001 ttttacttaa tttttaaaag attttccttt agaagctttt tgcgttttct ttattgaacc
    45061 cgtattcttc aaaaaaggct tctctaaatt caagcaaatt atcatccatg attgcttgac
    45121 gtacttgttt cattagattc aacaagaagt ataaattgtg gtaagaagtc aaacgaatcc
    45181 caaatgtttc atcacatttg atcaaatggc ggatataggc acgtgtatag tttttacatg
    45241 tataacaatc acatttctca tcaattggac ggaaatcatg ggcatatttc gcatttttca
    45301 ctactagacg gccttgagaa gtcatacatg tgccatttcg agcgattcga gtaggcaaga
    45361 cacagtcaaa catgtctact ccacggataa caccatcgat caatgaatca gcagcaccga
    45421 cacccatcaa gtaacgtggt ttgttttcgg ggatcaacgg tgtagtgaat tctaatacac
    45481 gattcatttc tgatttaggt tccccgacag ataacccgcc gattgagtaa ccagggaaat
    45541 ccatcccgac tagatctttc gcgctttgac gacgaagatc ttcaaaccct gctccttgga
    45601 tgattccaaa taatccttga cggtctggat tagcatgagc tttcaatcct cgttctgccc
    45661 atcgagacgt acgttctact gattttttca cgtagtcgta actttcatcg aatggtggac
    45721 attcgtcaaa actcatcatg atatcagagc ccaatttatt ttggatgttg attgcttttt
    45781 ctggtgataa aaacatacgc gaaccgttca agtgattttt gaaatgcacg ccttcttctt
    45841 cgatattccg catatcactt aatgaaaaaa cttgaaagcc gcctgaatct gttaaaatcg
    45901 gttgatccca gttcataaat ttatgcagtc cgccagcttc ttctactaag tcttcaccag
    45961 ggcgtagcca taaatgatat gtattactta aaataacgcc agcacccatt tctttcaact
    46021 cttctgggga cattgttttt actgtagcta atgttcctac tggcatgaac attggtgtgg
    46081 ggaaagttcc gtgtggcgtg atcaattccc ctaaacgcgc gccagtatgt ttttctttct
    46141 tgattaaacg atagcgaatg gcaggttgcg tcattcattt ccctactttc tatataaaat
    46201 atcttgacaa ctcccaccat cataatcgtt tcaagtgctt tttgcaagtt ccctcggctt
    46261 ccttctactt tatttaatat tctttttttt atcctttttt tatgaataac aaaaaaaaga
    46321 aaaacattat gacagaaata ttttttgata catggtatat tggtgttatt aaagaaaagg
    46381 agggaaaaat catgcaatct tctgcagaat taaaacgcca agcaaaagag agtctaaaag
    46441 gacgttgggg acaagctgtt ttactaaatc taatccctac attgattggt attgctacta
    46501 ttcttcttat tatgattcct attatcttgt ttgccaccac catatatgat ggatcagcga
    46561 gcgtctcaga agcgacaact tcaggaggcg gtagttcagg aggcggtggc ggaattattt
    46621 ccagcatcat tagcgctttg ttcatgagcg gtatttcttg gacttatctt gatattttac
    46681 gtggacaaaa agatcagatc gaaccattca aagatgcatt tcgtggattc aaaggtttgt
    46741 ttatcggcgg agtgatcagc ttagctgtgc tgatctcgat ctttacaaca ttatggggtt
    46801 tgctattagt tattcctgga atcattaaaa gctattctta ttctcaaagc tattttattt
    46861 actacgatat cgtccaagaa acaggacaaa cacctaaagt actggatacg atcactgcaa
    46921 gtcgaaaatt gatggatggt tataaaggac aacttttctg gttagatctt tcatttatcg
    46981 gctggcatat cttagcatta gcaactgctg gtattggtta tctatggctc agcccataca
    47041 tttctgcaac aaaagcagca ttttatgaac aattgccaaa agatatttaa aagagagtgt
    47101 gacttgtgac cgagggggtg gcaggagaac accgtttatc gagaggcagt aaaagaaaca
    47161 ttcagcccat tagcaaaaag gaggtgtgac aacattcatg tcacacctcc tttttgtata
    47221 gttccagtaa aaagaaaatc aacttagaaa cagttaatca ttcctcttct tctgcttccg
    47281 gtaatcgatg gaacttgatc agttcattca ccgcgattgg tacgatagat aaaatcccta
    47341 ccaagaacca gtgattcaaa ctgatgtgta ctaggccgaa tagttcttgc gtaaatggca
    47401 gcaaagcaac agccgttgta atagctaatg ccagtaacgc catttctacc aaagctttat
    47461 tggtcgctaa attgactttg aagatcgatt ggctgctgcg tacattgaac acatgcagaa
    47521 tcgatgaata agctaagatc aagaatgcaa cagtttgacc gacatgctga cttgcgtcta
    47581 cccatgggct gatagtatcg acataactac ctaaataata accaaacaag gttacgatgg
    47641 tgaagacccc tgcatttatg gcaatttttt gcaataaacc tcgagcaaag atcccttcat
    47701 ttttcggaat gggtggctga tccatgatgc cgtgttccgc tggctctctt cctaaagcaa
    47761 atcctggaag tccatcagca actacattga caaacagtag ttgcacagct gtaaatggtg
    47821 ctccccatcc taacagcatc gcaatcaaaa caataaagat ctccgaaata ttacagctta
    47881 gcaaaaagtt gatagcttta cgaatattct gataaaccga acgtccttga ctgacagcgt
    47941 ctacgatcgt cgcaaagttg tcatccgtca aaatcatatc tgcggcactt tgcgccacat
    48001 ctgttcctgt gatccccatg gcacacccaa cgttacttgc tttcaacgct ggtgcatcat
    48061 tcacaccatc cccagtcatc gctacgactg cacccgtacg ttgccaagat ttgacgatcc
    48121 ggatcttgtc ttctggcgtt acccgagcat agacagagag agctttgaca cgcgcatcta
    48181 attcttcgtc tgataattga tgcagttctg atcctgaaag tgcttctgac ggatcattca
    48241 agatacctaa ttctttcgct atggcactgg cagtcaccac atgatctcca gtgatcatga
    48301 ctgttttgat gccagccttt ttcgctcgct tgattgctcc tttactttct ggacgtggtg
    48361 gatcgatcat tccgatcaga ccaatcaaac gtaagtcttt ttccagtgct tcagaagaaa
    48421 tatcttgcgg ttcttcgtca taagtagcat aaccaacagc aatcactctc agtgcccgtt
    48481 taccaaagcg atcattaacg atcgccgctt gttctacatc gcctgaacta aagcgcggag
    48541 caagaacatc gaatgctcct ttggtgattg agatgtattt ttcgcccatt tggtgaactg
    48601 ttgtcatcat ttttcgttca gaatcaaaag gaagctcgtt tactcgtgga tatttttctt
    48661 ctaattctgc ctttgtatga taattctctt caacagccct tacgatggct acttctgttg
    48721 gattaccttt gataacaagg tcgtcatctt cttgctcgac cgtcacatct gtacatagtg
    48781 cagccatttt caatatttcc atcgcgctgt tcgtcattga atcttcgata ttcgttactt
    48841 catcttcttt gctccatacc cggcggacgc gcatttcatt ttgtgtcagc gttcctgttt
    48901 tgtctgagca gataacatta gcggttccta gcgtttcaac tgctggcaac tgtcggatga
    48961 tcgcatgttt cctcgccatc ttctgcacac cgtatgcaag tgtcaacgta acaataacag
    49021 tcaaggtttc cggtacagct gcaacagcca aggaaatcga agtcatgaac atctcaagga
    49081 gtggttctcc ttgcagttca ccaatcacaa atactaaagc cgcagccgct aaagcgatca
    49141 tactgattcg tttgcctagc tgattcaatc ggacctgtaa aggtgttttt tgcatagtat
    49201 tttcgttcaa aagatccgcg atcttaccca tttctgtagc catgccaatg gcagtgacga
    49261 tcgctttccc acgacctgca gcaaccgtac atcctttaaa taccatatta agttggtcac
    49321 ctaatgactc atcttcttca gcaataaatg ctgcatcttt ttcaactgct tcactttctc
    49381 cagtcaacgc cgattcttct acacgtaatt gtgatgcttc gatcaaacgg gcatcggcag
    49441 aaatagcatc accggactct aaaacaagaa tatcacccac taccagttgc tctgactcaa
    49501 cagtggtcac tttgccgtct cgtatgacag ttgccgtttg tttattcata tcttgcaagg
    49561 attccagtgc tttttctgcg tttccttctt ggacaatcgc caataccgag ttgatcacaa
    49621 cgatagcgat gatcagcagt ccttcaaata aatccccatg ttctgtagca aatgcggtat
    49681 aaaaggaaat caccgctgct acaagaagga tgatcgttgt aaaatcggac aagcttcgaa
    49741 agaatttttt gatcatcgat tctttcggcg cctcatcgaa tttattcaat ccctgctttt
    49801 ttcgccgttc atccacttct tgtgaagaca acccagtatc tggatctgct tctgttacct
    49861 tctttacttc ttctattgtc tgcttgtatt cttccatcct attcctcctt taaaatcttc
    49921 gtacaaaaaa aggtacttat tttactataa catccttttt ttatttactt caaataaagg
    49981 gcttatcgat tatttgttaa gaaagcgtaa aatttaaagt gcttatattt gcaaacgcta
    50041 ccaaaggatg ctattatata gatgtaagga aggaggatac catcaaatag acagaagaat
    50101 ttctcgatag aacagtgtaa gcaaacaaag ttgttagaag tacaggtttc acccagacat
    50161 gcgtgtagag tacaaaccta taaatagtga atgcgtatgg atgtttggat cacgaagtat
    50221 ccgaaacaag aagtaatgcg atacgtaaca attgaataac gtaaacgtct gaaaattgaa
    50281 taggaacaag agatgtttca aatgttgaag atgtaacgaa aacaaaattt ttgtttaaca
    50341 tgaagaaatt caacaccgtg ctttgcatgt atgatggaaa gttacaatca tttctgttta
    50401 tttgaatcct tccgaggtac aagaaagccg agcttatcga gagctcggct ttttctttgt
    50461 cataaaatct ttgtaaagct tttttgttat tcaaaactag cggcttttca taaccttatt
    50521 tcacgaacat cgcatctcca aaactaaaga aacgatagcg ctcttcaatc gcatgctggt
    50581 aagcagataa agtcaattct cttcctgcaa aggcactaac taacatgact aaagtcgatt
    50641 ttggcaaatg gaaattagtt gagaaagctt ctaccacttt gaattcatag ccaggcgtaa
    50701 tgaaaatatc agtccatccg ctgtctgctt tgatttctcc attaaatttc gttccaatcg
    50761 tttctaatgt tcgaattgaa gttgttccga cagccacgat tttcccgcca tttttacgta
    50821 cttggttcaa tcgctcagcc gcttcttccg tcaaacggta gaattcacta tgcatatggt
    50881 gttcttcaat tttatctacg ctgacaggtc ggaatgtacc taaccctaca tgtagtgtca
    50941 gatagactaa ttcaacaccc tttgcttcga tctcatcaag taattctttc gtaaaatgca
    51001 atccagctgt tggcgctgca gcagaaccat tttctttcgc atagacagtt tgatagcgat
    51061 ctgggtcttc cagacgttct ttgatatatg gaggcaatgg catttctccc aatgattcaa
    51121 gaatctctag aaaaattcct tcatactcaa agtcaatgat ccgtccgccg tgttccaatt
    51181 cttctttcac tactgcttgc aatctgccat caccaaaaga gattcttgtc ccaactttgg
    51241 cacgtttcgc aggtttgatc aatgtctccc acgtatctcc ttgtgtattc gttagaagaa
    51301 ggacttctaa atgcgcacca gtatctggtt tttctccata taaacgagca ggcagaactc
    51361 gtgtattatt cataactagc gcatcaccag gctgtaactc ttcaatgatt tcatggaaat
    51421 gtttatcttc catctctcca gttttccgat ccaaaacaag taaacgagaa tgatcgcggt
    51481 cttttaatgg tgtttgtgcg attaattctt ccggcaagtc aaaatcaaaa tcttctgttg
    51541 ttaacatatc gtcactcact ttctatcatg tgcttcatca ttctatcatg tttttcgttc
    51601 tgttggcaaa atcagcactt tttcacttct attttttgtt ttcctaaata aaaaaactac
    51661 cctatttgta ttttttcatt gttttaggca tactagtaat agatagttct tcattgaaaa
    51721 tagaaaaaga ggtggcacca tgattgaatt tcaaaatgtg tcaaaaattt ataaaggtgg
    51781 aaaagtcgct gtagaggacg tcaatctttc atttgaaaag ggtgagttca tttgttttat
    51841 cggtacgagc ggaagcggaa aaacgacgac gatgcggatg atcaaccgaa tgactgatcc
    51901 aacacaaggg aaaattttga ttgatggaaa atccatccaa gaaattgatc cagtggaatt
    51961 gcgacgtcag atcggctatg ttatccaaag tattggattg atgcctcaca tgacgatccg
    52021 tgaaaatatc actcttgtcc aaaaattgtt gaaagtcagt caagaagaac ggaataaaac
    52081 agctgaaaaa atgattgatc ttgtggaatt gccccgtgag atgttagatc gctacccaca
    52141 tgaattatct ggtggtcagc agcagcgtat tggagtcgtt cgtgcccttg cagcgaatca
    52201 agatatcatc ttgatggacg agccattcgg tgcgcttgat ccaatcactc gtgattcctt
    52261 gcaagacctt gtcaaagatt tacaagagcg tttgggaaaa acgatcgtat ttgtaaccca
    52321 tgacatggat gaagcgatta aactagcaag ccgtatcgcc atcatgagtg aaggccgtgt
    52381 gatccaattt gatacaccag aaaatatttt acgtcatcct gcaaacgatt tcgtagaaga
    52441 gttgattgga gaagatcgcc tactacaagc caagccaaat gcgacaagag tcggcgaagt
    52501 tatgctaaat acagcaatca cgattacacc ggaaaaatca ttacaagaag caatcaaact
    52561 gatgagggaa aaacgtgtag atacgttgtt agttaccgat aattcgaatg tattaaaagg
    52621 atttatcgat gtagaaacgt tgaataaaaa acgaggaaaa gtatccagtg tcggcgacat
    52681 tttgaataaa catgtttttt acgtaaaaga atccgcctat cttcgtgata ccttgcaacg
    52741 cattttaaaa cgcgggctga aatacgtgcc ggtcgttgat gaacagaaca aagtcgtagg
    52801 tatcctgact cgtgcatcat tagtggatat cgtctacgat gtgatttggg gagaagatcc
    52861 cgcaactact gatgttgcag agtcagctac tcctgaagaa tctaaggagg tctgacctat
    52921 gcagcagttt tttcatcagc acggttcaga aattctctca aaaggttttg aacatcttta
    52981 tatttcagga cttgctttga tcattggtat catcattgct gtaccgcttg gtgtcttact
    53041 gacacgtttt ccaaaagctg ccacgattgt gattggctta accagtgcct tgcagacaat
    53101 cccttctctt gctttattgg cattgatgat cccgctattt ggcgtaggta aattacctgc
    53161 gattatcgca ctctttattt attcattatt gccgatttta cgtaatacgt acattgggat
    53221 gaaaaatgtc gattggaatt atcgggatgt ggcaaaaggc atggggatga ctgagttcca
    53281 atccattttt tccgttgaat tgcccattgc tatgccaacg atcatggctg gtattcgttt
    53341 agctgccgtt tacgtgattg catgggctac acttgcctct tatatcggcg ctggtggttt
    53401 aggcgatttg atttttagtg gactgaataa ttaccaacca gatctcattt tggcaggtac
    53461 gatccctgta acaattttag cactattggc tgattggctt ctaggtctct tggaacaccg
    53521 gctgacaccg atcgcactta gagaggagaa tgaagaatga aacgtatact aaaaatgacc
    53581 atggttcttt tctctcttct tatacttgcg agttgttcct tccctggtct tgccagcaat
    53641 acagatgaag acaccatttc gatcacaggt ggaatcactt cagaagcaca gatcttagca
    53701 agtctagttg ccggaatggt tgaacattat acagataaaa ataccgcaat catcaacaat
    53761 ctggctacta caactatcaa ccatcaagcg atgatgaacg gagatgcttc aatttcagcg
    53821 gctcgctaca caggaacaga tttaacgact acattgaacc ttcctcctga aaaagatccg
    53881 aaaaaagcgt ttgctaccgt gaaagatgaa tttgagaaaa gatacggtca aacgtggttc
    53941 ccgtcctata gttttgaaaa tacgtatgtc tttcttgttc gaaaagacac cgcacaaaaa
    54001 taccatctat ctaaagtcag cgatctgaaa aatgtagcgg atgaattagt agctggtgta
    54061 gatacttctt ggatcaatcg taaaggagac ggttatgacg gcttccaaga aacttatggc
    54121 ttctccttca attcgatatt acctatgcag attggacttg tttatgacgc agtcgaagca
    54181 ggaaaaatgg atattgttct tggctactct acggatggac ggatcgccag ttatgatcta
    54241 gtcatgttgg aagatgatcg tcaattcttc ccaccatatg atgctgctcc agttgtagac
    54301 aacaagatat tagctgatac gcctcattta aaagaagcct taaataagtt aggtcatacc
    54361 atcagtacgg aaaaaatgca ggaattgaac tacgaagcag ataataatct agtggaacca
    54421 tcagtagtag cacaacgttt cttacaagaa aaccactatt ttgaagggaa gtgaagcaat
    54481 tggataaaat gaatgtcttt caacaatttt tctattattt ccaggaaaat ggctcctata
    54541 tctttgcaca atttatccgt cacttcctga tttctatcta tggtgtcctc tttgcagcag
    54601 ttgtcgggat tccggttggg atcatgattt ctaggagacg taaattagca aactgggtga
    54661 ttcgtttggc gaatatcatc cagacgatcc cttctctagc catgatctcc attttaatca
    54721 ttggattagg cctgggagtc aatgtcgtga ttgttactgt atttttgtat tccctcttac
    54781 cgatcatcaa gaatacgtat accgggatga tccaagtaga caaaaacatc cttgatgtcg
    54841 gcaaaggcat ggggatgact gctagacaac gactattcat ggtagagtta ccactctctg
    54901 tttctgttat catggcaggc ataagaaacg cgttagttgt tgccatcggg ataaccgcga
    54961 tcggtgcatt cgtcggtgct ggtggtcttg gagatatcat tatccgagga accaatgcaa
    55021 cagagggcac atcgattatt ttagcaggtg cattgccaac tgcattaatg gcgatcatca
    55081 cggactggct tttaggtatt ttggaaagac ggctagatcc agccagccgt acatcacgct
    55141 gatgttattg taaaaaacaa aagcttctgc gaccatttgt tggtcgcaaa ggctttttta
    55201 gttctgtcat ttttaaggca ataatccact ctgattcttc cctccttatg ggcaaagagt
    55261 tgcttttctt agctatttat ccattccgta tactccaact tcatactgcc aacgggttca
    55321 tatctgcatc caccgactaa gtttttttct tatccgctaa ggcattttgc caatccattt
    55381 tcccatcact cgtcatggaa ttcataccac atgatctcta aatactcgtc tgcttgttct
    55441 ttgaattggg catcaattct tacatccttt ggcgtggctt tcatttaagc cttttccatc
    55501 agtcctcgat tgttcgatgc cttcagctgc atcatcaaaa ctggtgactg ccgtatcggt
    55561 aacttcgatc atcttttata caatcgggaa cttgccatcc tcatccttgg ctaaaaacat
    55621 atctagccat tccagcttct cgccatggga ggtttgcggt tcctaaaacc ggtacacgat
    55681 tcttatggac tgcatccgtc gcaactagac ttggcgatac gatgatcctt caccagaaga
    55741 gcacccccgt aaaccaattg atcgatatat tgccaataag aaaagacatt ggcgtaaaag
    55801 atatcgatcc ctcgtgatgc attgccgttg atactgctat tcatggccga aagcgccacg
    55861 actttaactt cttccgtttg agtgacattc atgttcggca atgtcgcttt ggatacccgc
    55921 tctgctaaag gaacgacact ggtattgtat ttcgtatctg tatcttttcc aaaagaccat
    55981 gccaacgaat cctctgggaa ccaataagac gactccggtt gattgtccat tgcttttttc
    56041 aacatacttt tggctgtttc tcgataaacg acatttccaa ttgtcttcgc ctcactttgg
    56101 cgataatcga tggtcgttgc cactgccaac actaacaacg atgctccaat tttactgatt
    56161 ttccttgtca cacttcttcc tccttgtttt acataaaccc gtcaacattc cattcctaaa
    56221 ctcaaacaat gacccttcaa aggtggagat ataggaacaa attaaagaaa tcatgcaata
    56281 aatccaagaa ggttggtttt agaacaaaaa aagagtctcc tttttgggga gactctttgt
    56341 taactatcta aaaaggttgg ctcttctcac ttcactcata atgtgtccag gcagaataaa
    56401 tttctacaac tttattttcc tcatcaactt tgtaaaccac tcgatgctgt ctattcagcc
    56461 tgcgtgagta tctgccttca tgtgtaggtc tcagcttttc aaaagactgc gtgggcatat
    56521 atggatcttc cttcaatgtc tgaatcactt cttcaaattg tttctttaaa ttcgtttgct
    56581 tgatttttct taaatccact ttcgcagagt ttttgattgc gactgtatag ttactcatca
    56641 aagagcttcc caatcaatat catcgatatt cgtaaagcta ctgtcgtctt tttcgcggtc
    56701 tcgtacttta tctaaagtac ctgtttgctc aagaaaaagg gtttcttgaa tcgctctcca
    56761 gtcttccagc ccaatcaata cagcattgtt tccactacga tcactaataa tctcgatttc
    56821 agtatgattt tcatttacct ctttcaataa ctgataaaaa tttttacgag cagtgctagg
    56881 attcatcgtt gtattagcca ttgtctctcc tcctttttct gatataatta tagcgtactt
    56941 gattaagtac gtcaaaaaaa tacaataaag cacttaaatt cagcaaactt ttcctctttt
    57001 ttacatagag aaaaagaccc ctttttaggg aggatctttg tccacgctca cttcttactc
    57061 ctttacgact agcggtagcc aaacgtaaag taattggtaa caacgtatca tctttagcaa
    57121 aggtcgaatc caaagcctgt gagtctatgc aacaacacca atacacgaag gttcctcgtt
    57181 gcagtgtgac ttgattgata tcttgaataa cctgaggata cgcacgaaag atttgtaacg
    57241 gttattcaaa actgattttg gccgtcaagt cttctactgc ctcaaaaatg attgcctctt
    57301 tcgtctcctg tcattcatgt tcactatcga tttgatactt gcttagaccg ttgggtttgt
    57361 gaatcaccaa ttaaaaatgg cactgagtta tggtaacaaa aaatggtcac ttgacgaggc
    57421 tgagattcct acttatgaag acacatggca cttaagagtc gaggtaaagg aagaagctga
    57481 aaatatagga ggctgtacat tatttggcaa aggattcgga aattatgatc aagacatcaa
    57541 aatcaaaatg ttttacagct aaataggaag aataataaca tttcatacgc tgttcccatc
    57601 tttaatggct tatagagggc acctttagct gagaataaga caaaacagac cctctagctt
    57661 caattaagag ctagagggtc tgtttttgat tttaaaactt ggtttatttc ttcaaatggt
    57721 agctataggt gctgacaacg atattttcac gccaagacaa tcgcaatctt aaccgcaaac
    57781 ttgtttggtt atggagaata ttctgccagc gtctgtttgg tacaaattga ggaatcaaca
    57841 cagtagttgt atagttttgt ttcgctgcat ttttacttac gatatctaca taacggataa
    57901 ttggattttc aatcgagcga taagaagagt ggacgattga aaaacggaca tctgggaaat
    57961 gctttttaaa ttctgcttcg atttcttttt ctttttcgac atcttcgtct agcgaaacat
    58021 gcatcgcaac gacataatca ccgatcgaac gtgcgtaatt caacgcaccg atatttaccc
    58081 gtgtcacatt cccgactaat acaagaacgg tattcccgtc ataatcatgt agttttgcat
    58141 cattttctaa acgaagctgt tctgctactt ttttataatg atcatggatc ttataaaaaa
    58201 tgaacataac gattggcata atgatgaaga aaggccaaat gtcccctaac cgatagacaa
    58261 acaaaatcgc aatgattgca tatgagatga acgcacccgt aatattggca atcgatttgc
    58321 ttaaccatct ttttccttct tttttccatt tgacgaccat ccctgtttga gataaagcaa
    58381 atggaatgaa cacgccgata gagtataacg gaatcaagcg ttctgttgag ccatggaaaa
    58441 taaacagcaa tacgatcgaa cctagcgcta gtgtgataat cccattggaa tagcctagtc
    58501 gatccccacg atcttgatac atgtgaggca tgaatttatc ttttgccaga ttgtatgcaa
    58561 gaactggaaa agcagaaaaa ccggtgttag cagcaactgc taaaatcagc gctgtagcaa
    58621 attgaaggac gtaatacaaa ataccatgtc caaagacagc tttaccaatt tgagacaaaa
    58681 cagtgacttc tttttcagga acgatcccat accaatagtt gataaatgtg atccccacga
    58741 agaagaaacc taagatcaat gccatcatcg tcaatgtagc agcggcattt tttgctcttg
    58801 gcttcttaaa gaatggtaca gcattactaa tcgcctccac cccagttaaa gaggaagaac
    58861 cagaagaaaa tgctcgtaaa atcaaagcga tagaaacacc cggcacagcc gctcctggaa
    58921 gtgcagtagc ttgcaatggc acagcacctg tcacgatatt atataaacca acaacgatca
    58981 gtaacgagat cacaaggata aatgtgtaca ccggaaataa taggaacgat gcagattccc
    59041 tcagcccacg aagattcagc atcatcagta gcaaaacgat tgtcacagaa atagcgactt
    59101 gatgaccgta gagtgctggc accgctgatg tgatcgcttc agcacctgct gagacagata
    59161 ctgctactgt caacatgtag tcgatcaata aagagccacc ggaaatcaag ccagcattct
    59221 ttccaagatt ctcactgctg acgacatatg ctcccccgcc atgaggataa gcatggatga
    59281 tttgacgata tgatagcgtt aatgaaatca ataaaataat aacaaaagcg gcaatgggta
    59341 gcgaatacca gatggctgca gcagacaaag cgactaaaac gacaacgatt tgttccgtac
    59401 catatgcaat agatgataac gcatcagacg acaataatgc caaagcagca aatcgggtta
    59461 atttgtgctc atcattttca gcagatttta aaggcttacc tactaacaac ctttttaagt
    59521 aatccaattt ctaaactcct ttacacacaa ttttacttca agttttatag tcgaaaattt
    59581 atagtaaaca agatgttata gtacgctcac tgaccttaaa agtcaataaa ctttaagaaa
    59641 ataattggaa aaaaacagaa aaatatcaag cgctttttca aacgctttaa tacagcaaaa
    59701 atctaacatt tttttatggg ctttttcttt gaattctata ataaaaggat agaacttgac
    59761 ttagaaaatc aagttctatc ctttttgata tcttaacttt tggaagagag atgtaaggct
    59821 gctttctacc catcattctt actactcgaa gcatagcgca gttgtcacaa cctcttgata
    59881 aacggtgttt aaaagcttgc atctgcggta ataagcccaa ccaaattaaa aacataagaa
    59941 gctgttttta tttggtcgtt cttattactt gatgcagagc agctttttta caacctcttg
    60001 gtacacggtg ttcaatcagc tgaccctcgg tattagctca aaaaaacggc aaaatatgag
    60061 gaactatttt tccatttttt tctctaatac tcaggtcaaa acactgattt cacaacctct
    60121 ttttttaatg aaaaatatgg ctctcatgcg ccgcttcttt gaatttcgtc attttttctt
    60181 cgatgaacgg atagattgcc gttcctacga ttccaaagac aacaaacaaa ccgatcatga
    60241 tccagattgc tttcgtagca tttggccaat aaattcctcc tgcagattcc cgtaacaagt
    60301 ttaccgcatg agtaaatggc aagaacggat tgatcatttg gaagaatttc cctgagactt
    60361 ggatcggata gttcccgcct ccgccagaga tcgacagtac taagatgata atagcgatcc
    60421 ctttaccgac attatcgaat aatgcagcca agacatagac gatcatcatg aacgctaagg
    60481 cgatcaagac agcaaatgac acgctgtaga ccggattttg gacatctact cctagtaaga
    60541 aaatattccc taatgtaacg atcaagcttt gcgcaatcgc cattgtcaaa aatgtcagca
    60601 tacgagcaac gaattgttct cgtttcgtaa aattgtttcg gtctttcttt cctaaatagt
    60661 attctgtcgt agcaacactt gaaagaagaa cagcgccgac ccacagacat agtgcagtat
    60721 aaaacggggt gctagctgaa ccgttatttg cgattggata aagcgcattg gtttgtagct
    60781 caacaggttg tgtaaagaag tcactttctt ttgttgcgtc tagtttcaat aatttgatga
    60841 cttcgcctag atcgacttct tgttcgcctt ttcggatcgc gtctgctgct ttttggatac
    60901 cagctttgat atttggccag tcattgttga tcaagtccgc agcagcgttc acagctgttt
    60961 cgacttctgg cattttttca tttactgttg tcaatgtacc ggtgatttca ttttttagtg
    61021 ctggccaatc attttggata aaatcagcgg ccaatcctaa tttttgctga atgatcggta
    61081 attcattgtt gtataggtct gccccttcat tgatgccatt gacgatggta tccatatttc
    61141 cattcagcat tgtattggca tcatggattt cttgtccaat tgcaggcatt tctttttgat
    61201 atttttctaa aattgccact gcattcgtta cagttgcttt cgttgagctg agaagcgaat
    61261 caaagtcgat ctggtccgct ttgctcagtg cgccttgtgc agtcccaatc gtatcgatga
    61321 cttggtctag tagagaagtg acagtggaac tgatttgacc tgtattgata ccagcaatga
    61381 tgccattgat attattaaca acggcatcaa tttgatctag caattcatca atagccttcg
    61441 tgtcttcatt ttgaacagct gtgttcaaag cagataggcg gttttgcaga ttgcttagca
    61501 agggttttaa ttgatttagc tgatcaataa ttggctgtaa tttcgtattt ccagcacttg
    61561 tttgcaattt agtcaggaac tcgaccagtt tatcgattgc attttgttgt tgagatattc
    61621 cgtcaataaa gccgttcaat acattgctga tgtttttctt ttcatcatca gtcagcttac
    61681 catcttcaat agctgttctt acagtatcta ttaacgaacc tgtcgttgtt gccacttgtc
    61741 caattgactt caatgtaaca tctacactgt ttgtgatgct tggtatcgca tctttcagct
    61801 gagaggcacc tgattttgtt tggtttgcta gatcacttgc ctgattttct aaatttttca
    61861 catctggcaa gatatcttgt acttgttgga tgatcgtcaa gccttgtttt gcttcatcga
    61921 ttccttggtt cattgtctct tggatcgaag caaaatcgcc gtcgatctct gctagttgct
    61981 taccggcatt ttggatctct gggatttttt cttgcaaatc aaggatgatt tttgcttgat
    62041 ctttcaactc aggcattttg tcattcaatg caacgacttt ttcacccatc tcatctactt
    62101 tagggatgta atccacgaat tcatttgctt tattcagttt ttccttgatt tcaggtagtt
    62161 gactttgtaa ttcgaggact tgttttgtgt agccgtcgat cgtgtccaaa ttttcatctg
    62221 ttgataaaat catatcttta actttattga tacttactag gttcgtatcg atgtcgtaac
    62281 ctatctcatt gaatactttc aataaagtcg aactggctgt tttgataaat tcatttgtga
    62341 tttgcgattg gatcgaactt gctcctttat cggtgatttt cggtgcaatc gcattgattt
    62401 tttcatttac cgtatattcg atttttggtt tcttgatatc accactagta aaactcagca
    62461 gatcttctga gaaatccttt ggcaagtaaa tacctgcgta atactttccg gaacggactc
    62521 cgtcttctaa ttgttctttt gaatcaacga attgccatcc taattgttta tttttatgaa
    62581 gcgattcgat gacttgctcc ccaatggcca cttctttacc ttgaaactct gctggcttgt
    62641 ctgcactgta aacagcaatt ggcagctctc cagtatttcc atatggatcc cataatgctt
    62701 tgatgttaaa ccacgcatat agcgaaggga tgatcatgat cgcgatgatc agaagtgttg
    62761 caatcgggtt tttgaaaatc cgtttccagt caagcaaata taatcgaagc gtatttttga
    62821 ttgactgcat agttctcccc cttcttcttt tttcagctga attactttac catgtatttc
    62881 atactttttt caatcggctt ttttcaaaaa agtcgaattt ttggacatat cacggcaatt
    62941 gtctcattgt aatctcttac atattgagaa ataattaagg attacagcct ttactttatc
    63001 atgaaataaa agggaaagat aattttatgc caaagttttt atctattata cgtatacgca
    63061 aaaaagctaa aacatttttt gtctttgccc ctacaaaaat gtttcagctt ttttcgtttt
    63121 ttattcctgt atttcttatt tttcgaatga caggatgatt ttcccagcag gaggatgtcc
    63181 ttcaactagc tgatgtgctt ggatgacatg cttaagtgaa gcagacagat tcattgaaat
    63241 aaaaacatga aaggctcctt tttggtaggc tttgattatg ccagcaaagg cttctgcatc
    63301 tagatattcc tttcttggaa caaacgattc ataaaagcct tccttttttt gccgcaaatc
    63361 tagatctggg aggtcattta atgcaacata cctgccgcct ggtttcatga tctggatgcc
    63421 ggtttctcct ttgatacttc ctttcgtcgc atcgattaca aggtctgcct gatcagcaaa
    63481 ttgcagacct ggatttgttt tatcataagc agcaaatgct gaagctccta atttttttac
    63541 cttttcctca tttttactgg atgcactggt taagattcgg attccctttt catggagaag
    63601 ctggatcaga cttgacccga ccgcacctga tgctcctagt atcattacag tatctgtcgg
    63661 ctggatttcc gtcaaatggt tgattagatt ataagccgta attcctggcg tgaccattcc
    63721 ggctgcttct tcccaactca ttttatctgg aatttttgct acttttgctg atggcaacac
    63781 gacttcttct ccgtaagtcc cgccgactgc atggacagct actcgatcac ctacatgaac
    63841 agttgtgaca tctgaaccaa tctctgtcac aataccagcg ccatcattac ctggtacata
    63901 agggaatttc aatgttcgta tctctttcat ttctcctaaa cgcaaggcaa catcataagg
    63961 attcacactg aatgctttta tttctacacg aacatgtcca gaaccgactt ctctgggatg
    64021 agcatcgatt tcttcaaaaa catcttgtgc ttctccgtaa ccgttaattg caaatcttcg
    64081 catggtcacc atctcccttt ttctttaagt ttacgtttat gcctttattt tgacaaaaaa
    64141 aatgtctttt cgttttggcg catgattcaa aacaaaatag ttgtcttttt tcgcgtaacg
    64201 tgttaaatta tttatctaaa agcgtaaaga ttacgattta cgctaataaa aaataattgg
    64261 aggaatttgt tgatggctaa aaaatcaaag attgccaaag caaaaaaaca gcaagaaacg
    64321 attgaacgct atgctgcttt acgccaggaa ttaaaagcgc aaaaagatta cgaaggcttg
    64381 cgtaaacttc ctttagatgc tcgtccagaa cgattgaaaa atcgtgattt aatcgatgga
    64441 cgccctcgcg gatacatgcg taaattcggg atgtcccgta taaaattccg tgaattagca
    64501 cataaaggtt tgattccagg agtaaaaaaa gccagctggt aatccaagtc tggctgtaat
    64561 gtaaaggagg agggcgcatg gaccactcct tggatcatca aaaaagatac gttcgcttct
    64621 tctagcagaa atgctggtac tagaagcaga cgtatctttt tttacgcaca gctggtcctt
    64681 ttttatatgt aaaggctgca tggttacttc gctatattta tttagtaaca ggagtcagtg
    64741 cttcgatacc cacactaatt ttcaatgctc ggtcaacttc tgccataatg ggtcgatcca
    64801 aatggcagac cttttctttc aatcttgatt tatcgatcgt tctgatctgc tccaacagga
    64861 taacggagtc tctttctatt cctgttccag tagctttgat cccaatgtga gtcggaagct
    64921 taggtttagc catttttgcc gttatggccg caacaatgat cgtcgggcta aaatggttac
    64981 ctagattatt ttgaataacc aaaactgggc gtgttccgcc ttgctccgat cccacgactg
    65041 gagaaagatc agcaaaataa atatctcctc tttttaccat aggatcctcc ttattcttga
    65101 tattctcttg gtatccgctg tcctaatccg caagccactt catagtggat cgtacctaat
    65161 tgatctgcta tatcctgcat agtgatttct ttgtctgcat ttttgccgat aagcgtcact
    65221 tttgttccga caggaaattc ttgaggcaat cggatcatca gttgatccat acatacacgc
    65281 ccaatcgttt cacagtagtt cccttctacc aatagagaaa aaccctgcat ctttcgtggc
    65341 cagccatctg cataaccgat cggtatcgtt ccgatccact ctgcttcagg agtgatatat
    65401 gtttctccat agccgattcc ttcacctgct gggagttttt tgacttgaat caattcagaa
    65461 accagctcaa gtgctggctg caatggataa acttccggta gtgcgtgccc agagggatta
    65521 agtccgtaca ttgctactcc ataacgaatc atattgccga ttgtttcatc atgccataaa
    65581 gccgttgcac tattgctgac atgtacatat cgaggaagtt cgggaagttt tttcagcact
    65641 tctataaagc gttctttttg cagattccaa tatgtatcat ctgcctgatc tgctgtagaa
    65701 aaatgggtaa ataaaccttc ccattctaat acacgacttt ctttgatgaa tgcagctgcc
    65761 tcttttactt cttcaggtgt acaaaatccg atacgaccca tcccagtgtc tgcttttaga
    65821 tgaatcgaaa gaggcgtctc tagcttactt ttctctaaaa cagcagctgc tgcttgcaac
    65881 cattcccgtg tacctgcagt tactgataaa tcatgagcaa cagctacagt cagtgcgtcg
    65941 taaggaacca tattcaagat aaggatgggc tgagtaaatc cagcttctct caactcgact
    66001 ccttcatcta aatttgaaac acaaaagccc gaagctccac ctgcaactgc tgcctcagct
    66061 gtttcaatag ctccatgacc gtatccgttc gcttttacta ctgcaaacaa ttcttttcct
    66121 tgcggaagtc ttgcaacttc atttgctaca ttttcagtta tagctttttt atgtatcact
    66181 gctttcgttg ggcggtgcca agcgacaacc atcgtttttc cttctttcta gtcttccaaa
    66241 ataatttgtg caaaagctgt ttcatttgta tgagtaatac tgacccatac attgccttca
    66301 tgaggtgaac gcgtcacaac aggctgacca ttttcatttt ttaaaatctc gacatcttga
    66361 aaggtaactt ttccgatccc agttccccaa gctttagaga aagcctcttt gcaagcgtaa
    66421 cgcccgccta aaaattctac ttggcgatga aaaggcaagg actggaatag tttcatttca
    66481 tcagaggtca agattcgtgc aatgaatttt ggtttttctt cgatcagccg agtgatccta
    66541 ggaagttcga ctgcatcgat cccaattcct tttatcatat gcttttctct tttcaataaa
    66601 tttagaacac tctcattcta ccaaattata accattttgc cacaaataag tccacaaata
    66661 acaaaaatga aagtttctct taaaaaagag aaactttcat tttatgttta atcgttattt
    66721 gaacggatta caaatccgcg ttttttatca ttgtgacgtt taccgccatc tttttggctg
    66781 ttattatttt tgctataacc gccttttgat ttgtttccac gataattacc gccgtcacga
    66841 cgattacggt tgtttccgcc accacgtttg ccatttttat tgtatccttt tttctgcatt
    66901 ggcaatgggc gttctggtgt gatcttcact ggcactgcat cagctggatc ttttgctgtc
    66961 gtttttagca aaagtgcaac taaatcttgt gcagaatatt cttctaataa tttatctgct
    67021 gtttgcaggt atttatctag accattttct gcaagttttg tttcgatttg ttcaactgca
    67081 gcacctaatt gacctttgaa tgcttctttt tcggttggtg gacgtaaagt cgtcatccgt
    67141 tttttcgtca agttttcaat cacatgcaag tagtccattt catttggtgt aacgaaagtc
    67201 acagacatac cgccttttcc ggcacggcct gtacgtccga tacggtgaac gtagctttct
    67261 ggatcttgag ggatatcata gttgtacaca tgagtgacac ctgaaatatc taatccacgt
    67321 gcagcaacgt ctgtcgctac aaggatatct aaatgaccgc tcttaaatga acgcaatacg
    67381 ctcattcgtt tttgttgaga caaatcgcca tggattcctt ctgcacggta accgcgtgct
    67441 tctaatccgc gtgccaattc gtctacacgg cgttttgtac gtccgaagac gattgttaat
    67501 tcaggtgttt gaacatcaaa caagcgagtc atgatatcga atttttcgta ttcttttgcg
    67561 cggacatagt attgatcgat caagtcagct gtcatttctt tcgctttgat cttcacatga
    67621 tgtggatttt tcatgaattt aacgccaata ttcttgatag ctggcggcat cgttgctgag
    67681 aaaagtaatg tttgtctttg atcaggaact tgtgaaatga ttttttcgat atcttccaag
    67741 aagcccatat tcaacatttc atctgcttca tccaaaacta atgtttgaac ggttccaagt
    67801 ttcaatgtgt ggcggttaat gtggtcaagc atacgtcctg gtgtacccac aacgatatgt
    67861 ggacgatctt tcaagccacg gatctgacgg ccaatatctg ctccgccgta aacggcttgt
    67921 acacgtactt ttttgtcgcg ccctaaacga tataattctt cttgtgtttg gatagctaat
    67981 tcacgtgttg gtgcgataac taaaccttgt aattcgtgac gatctggatc gattttttct
    68041 aacattggta atccaaatgc agctgttttc cctgtacctg tctgtgcttg tccaataacg
    68101 tcttttccag ctaatgctag tggaatcgtt tctgcttgga taggcgttgc ttcttcaaaa
    68161 ccagcgcgtt cgactgattt caacaattct ggtgataatt ctagttcttt aaatttcaaa
    68221 tgaatcctcc tatatggttg ttaactgagg atgtagcttt tttgctaccc cgtcgatttg
    68281 ttcggtcaaa gtgtattgtt tctttttgac agtggccacg cacgttttta tccattctta
    68341 gcccctgtgg caccaccgaa aaatcacttt gcgaagttta ggcattagaa aagccaaacg
    68401 ggtcgcgttc agctttatca ttattatgac atctggcttc aacaagttgt ttctatggca
    68461 aaaccagtcc aatttctctt catctttcgg caataggtta ttctaacata gtttttcgtt
    68521 ttctgcaagt ttacagattt ttatttcatt tgctgaagtt gttctaccac ttcaaataag
    68581 cccatgctgt tgctgccttt tagcataata ctatctgttg cttcaagatt ttgtttgata
    68641 gcatcaatca atttattctt ttctttttca aaatagaaca caggtagttg ggggtatttt
    68701 tggttcaatt cttcttttag tgcatgcatt tgttccccat aaaggaaaat catggcgaat
    68761 ttctcaggat cgatatgttc actcatttgc gcatgcatat ttaaagactc tttccctaat
    68821 tccagcatat ctgctaacac agcgattctg cgtccttttg ttggcagatt tttaaaagta
    68881 tctaatacca gtcccatggc tgttggattt gcattgtaga catcgctcaa aatagctgca
    68941 ccatttcctg ccgttagcca ttcggttctg ttttgagtga tagaaacatg ggcgagccct
    69001 tggcgtatct caggtatgga cagtccaaag aagcgcccga tcgtatacgc aatcaaagca
    69061 ttcgttacat tataacttcc aggcagcggg atcgtaaatg tttctccttc tactacaaaa
    69121 tcggtttgtg cttttccttt tgctgtcact tccgcatgaa catcgccatt tccaataccg
    69181 aatgttgtga ctgtttgtac taatgttgcc gttaaaggtg tcagcaacgg ttcatctgcg
    69241 ggcacaacga gtaatccgtc tgatacaagt ccgtcagtga tttccatttt ggctttagcg
    69301 attccttctc gcgacccaag attttctatg tgtgcttcac cgatcattgt gattgctgca
    69361 gcttttggtt gagccataag tgaaagttcc gtgatttcat tggcgtgatc catgcccatt
    69421 tccaaaacga tcttttctgt atcttggggc atgtgcaaaa tcgtgtaagg aagaccaata
    69481 ttattgttgt aatttccttg cgtcttatat gttttgtatt tttcagacaa gacagacgcg
    69541 gttagatctt tcgtggtggt tttaccattg cttcctgtaa ttgcaacgac ttctgcgcct
    69601 actttttcca agtaataggt tgccaattgc tgcatggctt tcaaaggatc tgctacagga
    69661 ataactggaa aattttttgg agcttgttca acaggcaagc tccaaaatga agcagatgct
    69721 ccgtttgtga tcgcttgctg gataaattca tgtccatctc gcgtaccagc aagaggaacg
    69781 aataaacttc cttcggttat cttccggctg tcaaattcga cattggtaat ctttgtcagt
    69841 gcagagcatt cgctgcccac ggcttgtgca atttcttgta ttgttagatt cattgagatc
    69901 tctcctaaaa aaggttgtga aaaaagcgtt tggcctaaac ggcgggttcg catgactctc
    69961 acttccaagt aaatttctct attcgtattt agaagctaga atgaacgcgg ttacgcaatc
    70021 actccagcct cttctttatt tctctttatt catttttttc aaaaaaagat tgacgctgat
    70081 ggtatcgatt cattcctaac tggatcagtt cttcaatcaa atcaccgtat ttcaagccca
    70141 tattttccca taagagtggg tacatactga actccgtaaa tcctggcata gagtttaatt
    70201 cattcaggaa taattcattt ttatttgtca aaaagaaatc gcaccggctc aatccgcttc
    70261 cacctaacat cgtgtaagct aacttcgcgt actcttgcgc tttttgataa acttcttctg
    70321 gcacttcggc tggaatctgc atttcgattt tattattgat atattttgct tcataatcat
    70381 agaatgctac gtcttttacg acttcaccag gcaaagtcgt ccgaacgtct tcatttccta
    70441 atacagcaac ttcgatttcg cgcgcttcaa ttccttgttc aacgattgct cgagaatcat
    70501 actgataggc tgttgctaaa gcattttgca gctcttctcg gttttctgct tttgtaatgc
    70561 cgacactaga acccatattc gccggtttga caaacatcgg ataaagcaaa gaaccttcac
    70621 attgatcaaa tactttttta ggattttctt tccattgatt cttaagtact ggtacataag
    70681 gaacttgcgg cacaccagca gcttgtaaaa tatacttggt catgatttta tccatggcac
    70741 atgcactggt caatacgcct gcgccgacat aaggcatatt caatgtctct aagaagcctt
    70801 ggatcgttcc atcttccccg tttggcccat gtaaaactgg aaaaacgatg gctccttctt
    70861 ctttgatttc gcccggattg atcacttttc ctgtaaagcc ttcctctgtc tgtccacttg
    70921 ggtcccatga aagatgcaag acatctttgc tggcaggttt ttctgttagt aatggacctt
    70981 tgacccattg tccttctttt gtaataaata cgagttgaac ttggtaataa ttataataaa
    71041 tggcatttaa aactgaaaat gcggaaagaa tggacacttc atgctctgcg ctgcgtccgc
    71101 catatagtaa agtaatcttc aaaagattgt cctcctgtat tctaaatcgt tcagtgattt
    71161 actctcatat tttagcacaa aacaaaaata aacgaataac ttatttataa aaaaacgagc
    71221 cagcttctaa aagaacgaac gtcatttatt ttttgtcagc tagattattt gtcccgtttt
    71281 ttataaaaag ttatgataaa gaaaaggagg gatgatgatg gcgcaaacaa atccacgcag
    71341 ccgcttggca gaaatcattc gagtgttcat caattacaat gtagtgccaa actttgtaca
    71401 gcaaaaaaat cccgaacagg taaaaaaagc ctttgaagaa ctaggcccca ccttcatcaa
    71461 gataggacag atgctttctg ttcgagaaga tttgctttct tctgccttca ctcaaacatt
    71521 caaaacatta caagatagtg tacctagcga cacattttca acggtcaaaa agacgatcga
    71581 aacagaactt tctctttcat tatcagatat attcgatgat ttttcaaaat cgccttttgc
    71641 ctctgcttct atggggcagg ctcatcgtgc caagctgaag aacggtgacg cggtagtcgt
    71701 taagatccaa caccctaaca tcgccgaaga gatccgactt gatcttcaac tatttgaacg
    71761 agcgatccct ttgatcaaat acattcctga aactagcgta gttgatctaa aaggggtact
    71821 ccaagaagta aaacgttcct tgatcaatga aatggacttc ttaaaagaaa gtcaaaatgg
    71881 cgaacaattc tatcaaaaga acaatggctg gaaagagatc cgcagcccca agatttatga
    71941 cgctttttgt tcgaaaaaag tgatcgtgat ggaagaaatg tctggtaaaa acttgaatca
    72001 tttaatgaac gccgaaaaca aaactgaaac ttttatcact ggaattcaaa acaagcagct
    72061 gaaacaagaa gttgcaaagc ttttagttga gaactttatg aaacaggtgt ttgatgatgg
    72121 ttttttccat gctgatcctc atccagggaa tctgctattc catgtactaa caaaagaaga
    72181 acagacacaa gctagccgaa aaactgaaac agtccatgaa aaagagttcg gttcctttgc
    72241 ttttcgagcc tccacttctg cagaagaccc tgttgcgcca tataccatca actacatcga
    72301 ttttggtatg atggggcatc tttctgcagg acttaggcaa aaactcacac aagccgtttt
    72361 agcgctgtat acgaaagatg cttatcgaat cgaaaaagcg gtccttcgac tttgtcagca
    72421 agaaggctcg tttgatgaaa gtcggttcca ccaagaactg acaagttttc ttgaacagta
    72481 ttatgattct cctattgatg aaatcaactt gcaagaagta ttcacacatg tcgtgacgat
    72541 ttgtcatcaa aataatctgc agttcgatcg tgatatcacg cttttgctga aagcatttgg
    72601 gacattagaa ggggtgatcc gcgtcctcga tcctgaagtt tccctcatgg aagtcgcttc
    72661 gccttttgct caacattatt ttctcactca tctggatgta gaagatacat tgaaacaaag
    72721 cggattagac ttattggaag ggatgaaggc tgcgcctaag atccctcaac agctccacca
    72781 tctattagaa atgtggactt cgggacaagg aaaagtcaat ttggaattga aaaagcaaga
    72841 caaattattg agcaggatcg aaagtatgat caatcggctt gttttcggta tgattttagc
    72901 tgcattgatc gtagggtcct ccctgcttgt ccaagcagca cctgtagaaa acgcagaggt
    72961 cgtttcctta ttagggatat ttacgtatgc tattgccgca ttcgtcatta tttttttggc
    73021 aatcgatgca ttgatccaaa tgtataagaa gaggaaaaag tagaaaaagg ttgtgacaga
    73081 aagaatcagc ttcaagaaat aagaaggggt tttacataaa ttgtttctca aatttttgtg
    73141 aaaattcggc ttatttttcg aagaggctac ttctgaaaca ccgagaataa aaaatgtgaa
    73201 aaagctgttc tgcacccagt aataagaaca atcaaaaagg gaacatggaa cacatttatc
    73261 ctgtttccct gttccctttt tgatgtattc gagcataaat aaaatacctt cgttacctac
    73321 tatcttctgt acttggtaaa ccttagtcaa ttgccttttt taacgcttga cgaaaagaca
    73381 tatgatcttt ctccgtaaag ggttttggac cctttgtcct tatgccagct tttcttaact
    73441 ttccactatg ataacgttgt tctagaagcg tgccgatatt cgcttcgtca aattcttttc
    73501 cagagtgatg gaacactcgc acacccttcg gcaatacaag tgaagctaaa ccataatctt
    73561 gcgtgatcaa aaagtcattc ttcttaacca taccgacgat tttataatcc gcactatcag
    73621 ctccgcggtc tacgtagacg atttggacat gattagcgta ttctttggtc gtataatggt
    73681 ccacactggt cacgataacc acgtcaagac cataggattc tcctaatttt atgacatctt
    73741 cttttactgg agaaccatct ccatcaatga atagcctcat tctttttcgc cttcttccat
    73801 aaatgataaa cagttactaa tccgctcagt aaaagaccaa cattgatcca ttttgccaag
    73861 ataagcagtt gtacactttc tatcggcact gattcatggt tcagcgtgat caataataga
    73921 tggattggct ctcccatttc ttttgctaaa acaaacaaaa gcgtcatcaa taaaaactgg
    73981 tggatcacac tccctttatt ttgagtagta tacttgacaa cgatccggcg atccattgga
    74041 atagctgtat cagtttgata atattcctta tttagccagc tgcctgttac tacgtgccag
    74101 aaactaagca gaaagacacc tatcgcaaaa ccatttggaa tcaagaggac caataaagaa
    74161 gaactcagtc caagcaattg catagccaat ggagaccatg ttaaacgttt caaaatccat
    74221 ggtccgacta tttttgctgc aatcaagcct gctgcataag gcaaatacat aaaaatcgaa
    74281 agtttttcta atccgtacag tatgccaaca taaaaactgc taaataaaaa aaggaaattc
    74341 cctagcattc catttagaaa agtaaacaga tttagccagc ttggcaactt ccattccttc
    74401 ttctggtgtc ctaaaacaaa cacgaacaag atgaacagca gcaagaaacc ataaatagca
    74461 agatcaaaaa gcctttcatc gaataacaat cgggcatttc tcaaaagaaa caataagcca
    74521 aagaatatca aaaataaaac aagctctcgc gtagctacca gatttctttt gatgtctttg
    74581 aaatccagtt cgtaacgcgg ataatgacta accgtatggt aggccatgac gtaaaaaagt
    74641 gtataaatag taaagcttgc tgcaatctga gtcggtgcag gcaactcaat cgcttgatac
    74701 aggagaaata gccagatagc tgcaaataag tactttttcc catccatatt gataaaacct
    74761 tgcgtctttt cgtgaaaatt gaccgtggta ttagcaggta caagccatgc tgcactcagc
    74821 cctagcaaaa cacttgaaaa ataaaataaa ggaaaataaa atacgcctaa tattccaagc
    74881 aatgaaccag caccaccaat cagcagagcg atcatcagca acgtgtaact atctagacca
    74941 aatcgtagac tgttgactaa aaatagtcca gtcatccgaa aggcataaaa cagcgtaaat
    75001 ggcaaaatcg tcagccaatt atgcgagtct gttagtccca aaaataaaag ataaggaata
    75061 aaaataaccg tgttacttaa gataaagggc gttgtttgtg taaaattacg cccaaatcgt
    75121 ttgagttttt cgaacatgac aatggctcct taaacagtct tataactaat ctgctcttcg
    75181 attttagcat taaaggggaa ataaaccaat tttacagaat atattcttgt aaaaaaaaat
    75241 acagaaagaa gcataagctt ctttctgcat ttttacggta cttgattact caagcagaca
    75301 agcccttctc cacaaaagtc ttgcccgaca cccctgcatc cggcaggtga taaagataga
    75361 aatcaattat cctgtgcctc caaaatcaat taggagctct gtaaaccaga gtccttctcg
    75421 acccgtcgca taaatttact ttaacacagt tcctatgttt atgcgagagc ttttttcatt
    75481 ttataatcga ccatttgaaa tccttgaccg atatctgaaa taagttcttc tgtaatctca
    75541 aatccttcat gcttataaac ctcgattgct tgatgattcc cctgattcac tcgcaaaaag
    75601 atctctcttt cctttgtttg cgcagcaagt tcttcatacc agcgaaaaac tgaagaagtc
    75661 aatccttgac cacgtacttt atttagcaaa tataatttac ttaaataaat ttttccattt
    75721 atctcttcat aagcagtgta accgacagtc ttttcttcgc tttttaataa aaagtaggac
    75781 acaccttctg tcagttcttt ttgaatctgt ttttttgact gataagtttt cagcatgtaa
    75841 tccacttgtt tttgaccaat gatcggcaca aaaacttccg gccatacttc ttgtatgact
    75901 gccatcagct catccagttc attttctgtt acttttattt tttccataca cccacgtcct
    75961 tttttacatt ataaatgacc ttgcgcttga tggaataaaa agagcatgat catgctatac
    76021 acacccaccg accacggttt caagaaaatc acaatctgca taaattccct aaaagataat
    76081 ttcgtcaaac cagctactag acagatcaag tcgtctggcg caaagggaag cagcaaggta
    76141 acaactagta cttttttaat atctttcgtt tttcttgtca gcaattcatc aaatttctga
    76201 tacttttttg gcgataaaat aagctggaca aaggctacgc catagtaacg gacaagcaaa
    76261 aatccgattg cttccccaat cactaggcca atacaactat ataaaagccc aaatccattg
    76321 ccaaacatca gcatgcccgc tactgaggag atccctcccg gcaaaatagg aatgatcact
    76381 tggatcactt gcaacagaac aaatatcatc actgcatact tgccaaattg ctggataaac
    76441 gcttgcaaag acgtgctcga tcgaaaaatc ccatgatgat aagcataaag aatcaacaca
    76501 aagaaaaaaa acagaccaat aatgggcatg atctgaagac atttttgtaa aactgatcct
    76561 tttttagaca tctatattcc ccacatcttt tttcatttat taaaactgcc tcct
//
LOCUS       DACDEG010000004        76315 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.004, whole genome shotgun sequence.
ACCESSION   DACDEG010000004 DACDEG010000000
VERSION     DACDEG010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 76315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.004"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            31..1089
                     /locus_tag="IV142_000282"
     CDS             31..1089
                     /locus_tag="IV142_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP8483973.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            1108..2127
                     /locus_tag="IV142_000283"
     CDS             1108..2127
                     /locus_tag="IV142_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP8483974.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(2229..2945)
                     /locus_tag="IV142_000284"
     CDS             complement(2229..2945)
                     /locus_tag="IV142_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8483975.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(2914..3699)
                     /locus_tag="IV142_000285"
     CDS             complement(2914..3699)
                     /locus_tag="IV142_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8483976.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(3690..4439)
                     /locus_tag="IV142_000286"
     CDS             complement(3690..4439)
                     /locus_tag="IV142_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP8483977.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4737..5261
                     /locus_tag="IV142_000287"
     CDS             4737..5261
                     /locus_tag="IV142_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8483978.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            5277..6776
                     /locus_tag="IV142_000288"
     CDS             5277..6776
                     /locus_tag="IV142_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP8483979.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(6864..7466)
                     /locus_tag="IV142_000289"
     CDS             complement(6864..7466)
                     /locus_tag="IV142_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8483980.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            7570..8526
                     /locus_tag="IV142_000290"
     CDS             7570..8526
                     /locus_tag="IV142_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP8483981.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(8831..9787)
                     /locus_tag="IV142_000291"
     CDS             complement(8831..9787)
                     /locus_tag="IV142_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8483982.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            9961..10521
                     /locus_tag="IV142_000292"
     CDS             9961..10521
                     /locus_tag="IV142_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8483983.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            10514..11338
                     /locus_tag="IV142_000293"
     CDS             10514..11338
                     /locus_tag="IV142_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP8483984.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            11348..11965
                     /locus_tag="IV142_000294"
     CDS             11348..11965
                     /locus_tag="IV142_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP8483985.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            11983..12666
                     /locus_tag="IV142_000295"
     CDS             11983..12666
                     /locus_tag="IV142_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP8483986.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            13349..14584
                     /locus_tag="IV142_000296"
                     /pseudo
     CDS             13349..14584
                     /locus_tag="IV142_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            14592..15479
                     /locus_tag="IV142_000297"
     CDS             14592..15479
                     /locus_tag="IV142_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8483987.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            15496..16332
                     /locus_tag="IV142_000298"
     CDS             15496..16332
                     /locus_tag="IV142_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8483988.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            16335..16958
                     /locus_tag="IV142_000299"
     CDS             16335..16958
                     /locus_tag="IV142_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8483989.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            16955..18247
                     /locus_tag="IV142_000300"
     CDS             16955..18247
                     /locus_tag="IV142_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8483990.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      18606..18703
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            18819..19400
                     /locus_tag="IV142_000301"
     CDS             18819..19400
                     /locus_tag="IV142_000301"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP8483991.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            19403..20710
                     /locus_tag="IV142_000302"
     CDS             19403..20710
                     /locus_tag="IV142_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP8483992.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            21003..21491
                     /gene="purE"
                     /locus_tag="IV142_000303"
     CDS             21003..21491
                     /gene="purE"
                     /locus_tag="IV142_000303"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP8483993.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            21475..22608
                     /gene="purK"
                     /locus_tag="IV142_000304"
     CDS             21475..22608
                     /gene="purK"
                     /locus_tag="IV142_000304"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP8483994.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            22621..23916
                     /gene="purB"
                     /locus_tag="IV142_000305"
     CDS             22621..23916
                     /gene="purB"
                     /locus_tag="IV142_000305"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP8483995.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(24096..24473)
                     /locus_tag="IV142_000306"
     CDS             complement(24096..24473)
                     /locus_tag="IV142_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8483996.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            24729..25457
                     /locus_tag="IV142_000307"
     CDS             24729..25457
                     /locus_tag="IV142_000307"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP8483997.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            25457..25711
                     /gene="purS"
                     /locus_tag="IV142_000308"
     CDS             25457..25711
                     /gene="purS"
                     /locus_tag="IV142_000308"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP8483998.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            25713..26384
                     /gene="purQ"
                     /locus_tag="IV142_000309"
     CDS             25713..26384
                     /gene="purQ"
                     /locus_tag="IV142_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP8483999.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            26385..28607
                     /gene="purL"
                     /locus_tag="IV142_000310"
     CDS             26385..28607
                     /gene="purL"
                     /locus_tag="IV142_000310"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP8484000.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            28592..30031
                     /locus_tag="IV142_000311"
     CDS             28592..30031
                     /locus_tag="IV142_000311"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP8484001.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            30063..31106
                     /locus_tag="IV142_000312"
     CDS             30063..31106
                     /locus_tag="IV142_000312"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP8484002.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            31103..31681
                     /gene="purN"
                     /locus_tag="IV142_000313"
     CDS             31103..31681
                     /gene="purN"
                     /locus_tag="IV142_000313"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP8484003.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            31678..33213
                     /gene="purH"
                     /locus_tag="IV142_000314"
     CDS             31678..33213
                     /gene="purH"
                     /locus_tag="IV142_000314"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP8484004.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            33229..34503
                     /gene="purD"
                     /locus_tag="IV142_000315"
     CDS             33229..34503
                     /gene="purD"
                     /locus_tag="IV142_000315"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP8484005.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            34625..35539
                     /locus_tag="IV142_000316"
     CDS             34625..35539
                     /locus_tag="IV142_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP8484006.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(35672..37237)
                     /locus_tag="IV142_000317"
     CDS             complement(35672..37237)
                     /locus_tag="IV142_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8484007.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(37230..38681)
                     /locus_tag="IV142_000318"
     CDS             complement(37230..38681)
                     /locus_tag="IV142_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8484008.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(38695..39711)
                     /locus_tag="IV142_000319"
     CDS             complement(38695..39711)
                     /locus_tag="IV142_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8484009.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            39931..41019
                     /locus_tag="IV142_000320"
     CDS             39931..41019
                     /locus_tag="IV142_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8484010.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            41073..42599
                     /locus_tag="IV142_000321"
     CDS             41073..42599
                     /locus_tag="IV142_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP8484011.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            42601..43806
                     /locus_tag="IV142_000322"
     CDS             42601..43806
                     /locus_tag="IV142_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8484012.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            44063..45040
                     /gene="guaC"
                     /locus_tag="IV142_000323"
     CDS             44063..45040
                     /gene="guaC"
                     /locus_tag="IV142_000323"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP8484013.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            45304..46728
                     /locus_tag="IV142_000324"
     CDS             45304..46728
                     /locus_tag="IV142_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP8484014.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            47189..48298
                     /gene="pdhA"
                     /locus_tag="IV142_000325"
     CDS             47189..48298
                     /gene="pdhA"
                     /locus_tag="IV142_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP8484015.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            48301..49278
                     /locus_tag="IV142_000326"
     CDS             48301..49278
                     /locus_tag="IV142_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP8484016.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            49303..50946
                     /locus_tag="IV142_000327"
     CDS             49303..50946
                     /locus_tag="IV142_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP8484017.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSASSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            50954..52360
                     /gene="lpdA"
                     /locus_tag="IV142_000328"
     CDS             50954..52360
                     /gene="lpdA"
                     /locus_tag="IV142_000328"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP8484018.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            52522..52797
                     /locus_tag="IV142_000329"
     CDS             52522..52797
                     /locus_tag="IV142_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP8484019.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            52803..53570
                     /locus_tag="IV142_000330"
     CDS             52803..53570
                     /locus_tag="IV142_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP8484020.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            53738..55567
                     /gene="typA"
                     /locus_tag="IV142_000331"
     CDS             53738..55567
                     /gene="typA"
                     /locus_tag="IV142_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP8484021.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            55828..57600
                     /gene="recQ"
                     /locus_tag="IV142_000332"
     CDS             55828..57600
                     /gene="recQ"
                     /locus_tag="IV142_000332"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP8484022.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            57714..58016
                     /locus_tag="IV142_000333"
     CDS             57714..58016
                     /locus_tag="IV142_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP8484023.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            58019..59182
                     /locus_tag="IV142_000334"
     CDS             58019..59182
                     /locus_tag="IV142_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8484024.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            59208..62636
                     /locus_tag="IV142_000335"
     CDS             59208..62636
                     /locus_tag="IV142_000335"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP8484025.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            62657..63781
                     /locus_tag="IV142_000336"
     CDS             62657..63781
                     /locus_tag="IV142_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484026.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            63771..64208
                     /locus_tag="IV142_000337"
     CDS             63771..64208
                     /locus_tag="IV142_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP8484027.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            64192..64530
                     /locus_tag="IV142_000338"
     CDS             64192..64530
                     /locus_tag="IV142_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP8484028.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            64649..65209
                     /gene="rsmD"
                     /locus_tag="IV142_000339"
     CDS             64649..65209
                     /gene="rsmD"
                     /locus_tag="IV142_000339"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP8484029.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            65206..65697
                     /gene="coaD"
                     /locus_tag="IV142_000340"
     CDS             65206..65697
                     /gene="coaD"
                     /locus_tag="IV142_000340"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP8484030.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            65687..66730
                     /locus_tag="IV142_000341"
     CDS             65687..66730
                     /locus_tag="IV142_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP8484031.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            66889..67617
                     /locus_tag="IV142_000342"
     CDS             66889..67617
                     /locus_tag="IV142_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP8484032.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            67733..67978
                     /locus_tag="IV142_000343"
     CDS             67733..67978
                     /locus_tag="IV142_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8484033.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            67975..68193
                     /locus_tag="IV142_000344"
     CDS             67975..68193
                     /locus_tag="IV142_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP8484034.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            68329..68868
                     /locus_tag="IV142_000345"
     CDS             68329..68868
                     /locus_tag="IV142_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP8484035.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            68861..71134
                     /locus_tag="IV142_000346"
     CDS             68861..71134
                     /locus_tag="IV142_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP8484036.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            71172..72218
                     /gene="holA"
                     /locus_tag="IV142_000347"
     CDS             71172..72218
                     /gene="holA"
                     /locus_tag="IV142_000347"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP8484037.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(72307..72561)
                     /locus_tag="IV142_000348"
     CDS             complement(72307..72561)
                     /locus_tag="IV142_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP8484038.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(72961..73230)
                     /locus_tag="IV142_000349"
     CDS             complement(72961..73230)
                     /locus_tag="IV142_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484039.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(73700..74155)
                     /locus_tag="IV142_000350"
     CDS             complement(73700..74155)
                     /locus_tag="IV142_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP8484040.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(74293..76221)
                     /locus_tag="IV142_000351"
     CDS             complement(74293..76221)
                     /locus_tag="IV142_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP8484041.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT        24722 a        13188 c        16578 g        21827 t
ORIGIN      
        1 gaataaatga acataattag gaggagaaag atgaacttat ttgacatttt tcgatcgact
       61 ttgacagata tgtcgattgt cagtgcaatc acttcaacag tttttattat attattaggg
      121 tttgtttgta gaaaaaaggg gattttttct gaatctgttg gaaaaattct atcgaaagtc
      181 gtcttaacag tggctttgcc agcactggca tttcgagctt tcatgcaaga tatcaataca
      241 gaattactga tgcaaggaat caatgtcttg atttggggat tagttattta tattattctt
      301 attctagttt caaaaccttt tttcttaagc tataaagggg ataagcaaac aacgttgcgt
      361 gtcctgacga tctttggttc tacgaccttt ttcggaacgc ctatcgtcag tgcgatttat
      421 ggtcctgtcg gcgtgatgta ttcctcaatt ttcaatattg gctaccgaat ctttttgtat
      481 tcttatggat acataaaaat gagtggcttg aagatggaag ctaaaaatat caaagcgatg
      541 ttcgtcaatc caatcattat cgctactttt gccggccttt tcatttggct gttccaaggg
      601 tatctgccac aagtgtcagt tacaggtgca gaaggagaga taagccaagt agcgtttttg
      661 cgaattgacc agactgcacc gtggctattt cagccgatgg attatttagc tggtttaagt
      721 tcaccattgg cgtggctagc aatcggcgca acattaggtg aagtaagctt caaaaaagct
      781 gcatctgaaa aaacttcatg gtattacagt ctagtcaaag tggtatttgt acctgcattg
      841 aatatcgtga ttctagcagt tttgacggca acgaatattc taccagtcaa ttttgaagca
      901 ctggcaacaa tcgttatcat gatggctaca ccagctgcaa cagtggcagc agcctatgct
      961 atcagttttg atagggaggc acttttagcc tccaatgcat ccttactgtc aactattttt
     1021 gctgtggtac tgacacctgt atggatcgtt gtattacaaa tgattcatgg cataggttta
     1081 ttttcataag aaatggagga agaaagtatg agaatcgcat gttacggtgt tcgtccgaac
     1141 gagatagatt tttttgaaaa attgaatatt taccattatg atctcagtct ttatgaagaa
     1201 ctgttgacac atgacaatat cgtgtcagca aaggaccatg atgctgtatt gttgagggga
     1261 aattgtatag cagatgagac caatcttgca aaaatgcagg aatatgggat tcgttatgtt
     1321 tttacacgga ctgtgggagt caatcatatc gatctacaag cggcagctga ttttggtatg
     1381 acagtagccc gtgtaccatc gtattcgcca aatgcgatag cagagttatc tttgactttt
     1441 gccatgatgc ttttgagaaa tactgcgtat acgacgatcc gaacatcttt caaagatttt
     1501 cgagtggatg agcaaatgtt tagtcgtgaa atcagaaatt gcacagtagg gatcatagga
     1561 acaggcagga tagggttgac ggaagctgtt ttgtttaagg gactaggagc caaagtgatc
     1621 ggctacgata ttcatcagac agaagctgct aaaaaagtat tgacgttcca ctcgcttgaa
     1681 gagttattag ccaaaagcga tattgtcagt attcatgtac ctcatatccc aggtgaaaac
     1741 gatcaaatga tcaatgaagg atttttagct caaatgaaaa aaggtagcat cttgattaat
     1801 acagcccgtg gagagcttca agataatgaa gctattttaa gagctttgaa aagcaatcat
     1861 ttagctggtt ttgcaaccga tgtttttgca aatgaaacag aggttttttt ccgatccttc
     1921 aagccatggg agacgattcc agaccctgct atccagcaat tagtagaaat gtaccctaga
     1981 gttcttgtaa ccccgcatgt aggttcaaat acagatgagg cattatcaaa tatgatttcg
     2041 acaagtttcg agaactttcg tgaaataata gaaactggaa aaacgaaaaa tgaggtatct
     2101 cttcctaaag cccgtcaatt aagataagaa aacatggata ttcgtcaata aaatgtttaa
     2161 aaatagtcca agtaaacagt tgttacttct gtatacttgg actattttgt atgggataat
     2221 tcgatgactc aacagtaatc tgtgagcttt ttccgcccat tctttgtcaa ttcataacag
     2281 gttagcggct ggccgatttc caaatatttg attctttcgt tcaatatatc ttgttctact
     2341 aaaaaattca aatatttctt gacggatata cgtgacagtg agcttttttg agccagttct
     2401 tttgttgaaa aaatagtatc ttcttttaga ataaatgcac ttattttttt taaagtcagt
     2461 tttgacagac ctttaggcaa atcgatgaat gatggtattt cgtgaatggg agtggtgtgg
     2521 ttgaatactt gatcgatcaa tttctgatcg gctttttcag tcgtttggat tgtctgagag
     2581 aaagcaagat acttttcgat cgccagctga aaacgttgca aagtgaacgg tttgatcaaa
     2641 taatctaagg caccgtagcg gatcacattc tttatggttt cgatatcatc tgcagcagtg
     2701 atcaaaatca ctgggatatc atttccttgc tgtctaagcc aagataaaaa atggatacca
     2761 ctttcgccag gcatgtatac atcaagcagc agcagatctg cgtttttttg tctgaaatat
     2821 ttttttcctt cttctacatt tctgaaactt tcgattacgt tcatattagg gatgctttcc
     2881 acaaaagtac gattcaattc tgcaaccatt ggatcatctt cgattatcag gacatttgtc
     2941 atcattttga tcttcctttt ttggtaattc tagatagacg attgtaccgc catttgtttg
     3001 tttggaaaat tctaaaaatc caccgtggcg tttgacgatt gaatggatga gattcaatcc
     3061 aaacccacga ttgattccct tcgtcgaaaa acctagcgta aatatttttt ctgagatttc
     3121 ttctgatatc ccgacaccgc catcgcagac ggtcacgatg aatacttcag cttctgtgtc
     3181 atagtttaaa tgaatccgga cctgtccgtt tctttcatgt ttttgtagca cggcttcttt
     3241 tgcattatca agtaagttcc ctaacacttg caaaagtgaa tggatggagt catccatctc
     3301 cattttaggg ataaaagaat cgctttctaa cagcatttca acagattgtt ctttggcttc
     3361 gtttattttg cctaataaaa aaccggcaat tgcaggtact ttgatcagac ttgagacttg
     3421 gccaatctct tttttataat tttgatttag ctgctggata aaatgggtga cttcttcata
     3481 cttcttcaaa tcaatcaaac cagaaatgac atgaagttta ttcatgaatt catgggtttg
     3541 agctctcatg gagtttatat attgtttcgt ccccccaagc tcctccatta atttcattac
     3601 ttctgatcga tttcgtaaag ttgcgactgc tcctaaaaaa cggtcattgc taaaaagagg
     3661 tgtaacgcta gaaatagttt ctatcccatt taaatacatg gattgagtcc gaacggtcgt
     3721 ttgatgctca atcgtctctt taaacaaaac gtcatataga ttatcactga tttttttcct
     3781 acttcgaaat ccaatagctg ctctgtttgg tagaaaatgt tttttgcagg tgtattgatc
     3841 aagaggatag ttttatcaga agagatggct aagattcctt catctacttc attttcgata
     3901 atcgtttttt cacttaactg ttctgctatt tcttgtggtt caagtccaag cagtactttt
     3961 ttcatttttt gggcaagaaa aacggcgcct atgatgccta cgctaagtcc aataatcaat
     4021 gaaaaaacaa tcattttttg tccatctttg acttgattgt caatcgtgtt gtttgttaag
     4081 ccaacacaaa ctgcaccaat aatctcttga tcataataaa tagggacgaa atagcggaag
     4141 ccgaacccta acattccttc tttttcagag aaaaattctt gtcctccaat cacttttttc
     4201 acatcttcat gacttgagaa aggaagaccg atgtaacttg ggtctgcgtg agaatatcga
     4261 atgccttcag ggtccatgac aacgataaaa tctaaattcg atgcaatcat gatttcattt
     4321 gttattcctt gaatcgcatg tgtcgggtca ccagtctgca gctttttttg gatgagttta
     4381 tcattggcaa cgattttagc aatgctagag attttatctg acacactaga tcggttcatc
     4441 gaaagaataa aattcctgat caatatggca gaaacaatca aagaaacgaa tgtggagaaa
     4501 caaacgacaa ggatgatcgt gttctggagc tttaatggtt ttttcttaaa aaaaggaaac
     4561 ataggctacc tcgaaagaat ttattgtgaa aattttaaca tgaaaacaaa aatttaacaa
     4621 attgtcaacg ataaacacaa ccttcctata aaaaacacca ctgagtcggt aaaagaagct
     4681 atacagaatt ttcaaacatt ggtatgatag acgtagaaat ggaaggaggc gtgtatatga
     4741 tagggggaat cgcacttgag acgatttatt ggtatacatt gctgatttgt gcaggattgg
     4801 cagtcctatt gattattttt ggagatgtat tcgattttga tggcccgctt gatccgatgc
     4861 ttattatccc ttggttaact ttcacaagct tatttggttt tctaggtgag cgtttaacag
     4921 atcaatcatc tttattttta tttattgcta gtggaggcat atcgatagtg ttggtatttt
     4981 tgcttaattt ctatatattg atgccgatga aacatgccga atcaacgatg tcgtcttctg
     5041 aaaaaactct ggaaggacaa gtagctgttg tgatcacacc gatccctgta cgaggtatgg
     5101 gagaaattca gttgaaaagt gttaccggtt ccattagtcg tccagcttgt ttttacagcc
     5161 cccaagaaca gacagctgag cggggaacga aagtattgat cattgagatc aaggaaagaa
     5221 tctgttatgt catgccttat gaaagcatga taaaagctta aaggagcgtg tgttaaatgg
     5281 aaataccagt tgtttttatc gcgatcgcaa tcgcagcttt gattttactg atgttgctga
     5341 tcgtctttgt agcgaagtac cagacagcaa aaccggatga agcgttgatc atcagtggaa
     5401 gttatttagg aagcaaaaat gtgcacattg atgaaagcaa taacaaaatc aaaattgtgc
     5461 gtggaggtgg cgcattcgtc ttgccagttt ttcaacgttc gaatcggatc agtctattat
     5521 cgagtaaatt ggacgtctcc acccctgaag tgtatacgga acaaggcgta ccggtgatgt
     5581 gtgatggtac ttcgatcatc aaaatcggtt catctgtaga agagattgct acagcagcag
     5641 aacagttttt aggcaagaca agagaagaac tggaaaatga agcaagagaa gtattagaag
     5701 gacacttgcg ctcgattctt ggttctatga ctgtagaaga aatctatcaa aatcgcgata
     5761 agttcagtca aagcgtacaa gaagtcgctt ctgtagacct tgcaaaaatg gggttgatca
     5821 tcgtttcttt cactatcaaa gaggtcagag acaaaaacgg ttatcttgac tctttaggga
     5881 aaccaagaat cgcccaagtc aaaagagatg cagatatagc agaagcggaa gctctaaaag
     5941 aaacgcgtat caaaaaagct caagcagaaa aagaatcaca agcagctgaa ttacagcgac
     6001 aaacagagat cgcagaatca ttaaaagaaa aagaactgaa attagcaacc tacaaacaag
     6061 aacaagatgt tgctaaagca aaagcagacc aagcctacaa tttagaaagt gcccgagcac
     6121 aacaacaagt aatcgaacag gaaatgcaga ttaaaatcgt agaacgccaa aaacaaatcg
     6181 aactggaaga aaaagaaatc acacgtcgtg aaaagcaata tgattcggaa gtgaagaaaa
     6241 aagcagatgc cgatcggtac gctaaagaac aagaagcaca agctcaaaaa gtcaaagaag
     6301 tgactgaagc cgaagcagaa cgattccgtg tggaagcact ggcagaagcg gaagccaata
     6361 agacacgttt agctggacaa gcagaagccg aagcagcact agcaaaagga aaagcagaag
     6421 ccgaagcaaa acaaaagatc gccaatgcct tcaaggaata tggcgaagca gctgtcttga
     6481 gtatggttat cgatatgcta ccgcaattga tgcgcgaagc agcacagccg ctaggaaata
     6541 tcgaaaaaat ctctgttgtc gatacaggaa gcagttcagg agaaaccagt ggagcgaacc
     6601 gagtgacaaa ttatgccaca aatttgcttt cttctactca agaaacacta aaagcgacaa
     6661 caggacttga cttgaaggaa atcgtagaga atttgtcgac acgtgggact actagccaag
     6721 taaatatgta tgaagcgcca aaagaggcag aacaaaactc gatgaaggaa gaataaagta
     6781 gtacacttca ccgttcaatc aaaaatggag gaagactttt taaagttctt ctctccattt
     6841 ttttatttta ttgcaaaaga aaattagtcc tcgctatttt tcttttgttt ttggatctta
     6901 gcttctatct cttgtaaatt tgttcttagc aatcgttctt tcttttggag ttcgaccaaa
     6961 tcttctgata gttcttcctt cttttctgtt tcaggaggac gaagaaagaa attgataccg
     7021 attgcaatga atgtccctat aaaagtatct agtacacgtt ggatagcata caaagcggat
     7081 tcgccttgag ggatgctcaa cgcaatcagc aacaacgtag ctattgcgga aatgataccg
     7141 gaattattgt taataccatc tgacacgacg atcacaataa taactagaat aggaagcaag
     7201 atcaattcta ccaaaaaatc attgtgaaaa tagcttttga caaaaaaata aatgatagct
     7261 aatgtaccac ctaatgtatt gccgatgatc ctagactttc caaaagaaac ggttgtagtc
     7321 aagtcctgac gcaaagaaaa aacagcagat aatgcagcaa tcatcgggat tcctcgttga
     7381 gtcaaatgaa aaaacaagat acataaaaaa acagctaagg cacttttgat cgtacgcata
     7441 cctagtcgaa aacggccaaa ttgcatagag attctccttt ttggtctttt tactatcata
     7501 cctaaacttt tgtctgatag ctagtttttc attattcaag tataattaaa ataaaaaagg
     7561 gggaagtaaa tgcgtatagg aatgcgaaca gtcaaaacag tgatcagtgt cttcttttcg
     7621 atggtcacag cgggtagctt atcgctgttg tattggccag ctgcagggat cattgcatta
     7681 ttaagtgtag gaaataccaa gcgctctaca ttgcgtacgg gcatgaatcg aatcgcagcc
     7741 tttatattag ctacggcaac agcactgttc agtttttttg ctgttgggta tactattttt
     7801 ggcttcagtc tttttttatt cctgttcatc ccgcttgcct ctcgcttcaa attaaccgaa
     7861 gggatagtag taaattcggt tttggtcact cattatttag tggaggaaaa catgtcttgg
     7921 cagctgatcg gcaatgaagc gggattaatg gcgattggat tagtatttgc tttgcttgca
     7981 aatgtttaca tgccagatcg tacgaagcag ctgaaggaaa accaaataca gatcgaaaaa
     8041 gaatttcgaa atattttgag acaaatggca gaatttcttt tatcagaaaa taaagaagat
     8101 gtgcagatca aatgcgaaca tttactgaca ttcattcgcg aaagccaaga agatgccaga
     8161 gaacatcaag aaaactactg gctcagacag ccgctatact acgaaactta cttctctatg
     8221 agaagagcac aggccaatgt gattaaagac atgctggaaa atctagagag aatccagcaa
     8281 ccagcttatt atggaaaaca tatctatggt ttattgattt atactgcaga aacattttca
     8341 gagtcaaatg atggcagaca aattttaaca cgtattgaag aagtttatgt attgtatcgg
     8401 caaatgcctc tgccgactag tcgccctgaa ttcgaggatc gagcagaatt gttccagttt
     8461 ttgcagtctt ttaaaagttt tattgaaatc aaggcagaat tctcccaaca aaaaatccaa
     8521 aaatagaggt tgtgaattca gctaatattc aggtcaaaaa gctgatttca caacctcttc
     8581 tttaaacggt gttctcctgt caactcctcg gtcacaagtc acaatattca acaaacatga
     8641 gaagctgttt tttgaaattg ctccttgtga ctcggagctg aacggtaggc tcacaacctc
     8701 tttcttaaac ggtgttcaaa agctttcctc tgcggtaata agtccagcca aacgaaaaat
     8761 aggaggagct atttttgttt ggctgttctt attacttgat gcagaacagc tttttcacaa
     8821 ccttttaaaa ttacatattt ttccatagaa cttgcatttg atccaagatt tcgatgacat
     8881 cttttgtcaa gaagagagag cgattgggca agtcaccttc aatcatgtgt accatattct
     8941 ttatttcgta attcattgct tcagatgtat tcccgctttc gatgaattcc tttgtaccat
     9001 ctgtaaataa gatctccgca cgatcagcac gcgggtaatc agcaatcgtc acataaccgt
     9061 tctcaaaggc aacgataccg acttttggca tttttgcctg aaaagtcaag ctgacagcag
     9121 caagttcatt ttctttgtta cgcaagatag tgacggattg ttcgtccacg cctgtttcaa
     9181 aaggaaccat tgtagaggca actacttcag gttgagagct caaaaagaaa cgagcgaaag
     9241 atacagcata cgtaccgata tccaacagag ctcctccagc taattctgga ttgaagaacc
     9301 ggtttttagg gtctggttct ttgtaactgc cgaaaggtgc ttggatcatt tttaaagccc
     9361 ccagttttcc tgtatccatt attgagcgta actgctgata aagtggcatg ttgaagattg
     9421 tcatcgcttc tgctaagatc aaattctttt cttctgctaa gcgcatagcg tcagccagtt
     9481 cttttttatt catcgtgatg gctttttcac atagaacgtg tttgcctgct tccaatgctg
     9541 ccaagatatg gtcgatatgc tgtctatttg gtacagcaat gtacacgatg tcgatttcag
     9601 catcgtctaa catttcttga tagcttccgt aagcttttgg aatattgtaa cggtgagcga
     9661 agttttccgc tttttctgaa gttctggaag caactgctgt tagttcgctc gtctcttgat
     9721 tgaagctttc ggcaaactca tgtgcgatgg taccaagacc aacgattccc cattgatatt
     9781 ttttcatctg aaaacgctcc ttcatagttg gattgagttt cttgtctgtc tattattata
     9841 acaaaaatca cgaagaataa agaggagagg acttgaaaca ttaaaaaaaa tgaaagcagc
     9901 gatttaagag gtgctttttg atcaaaactt aagtagaata gttaaaagga ggggataaac
     9961 gtgcatcaag tatatattga tcttattatg agtgtattat tagaacagtt tgaaactgaa
    10021 aaaaattttt tagatgaata cttgctgatc agtccagaac aatggcaaaa atggaaagat
    10081 ggcgagaaaa atctgccatc tgaagcgatg cagaaagtaa aaaatctttt cactgattat
    10141 gaatggatgc taacgcaaaa gattttgcgt caaacgatta tttttcccga aaaacgaaac
    10201 gttgccgttg cagagtacaa gcaaatcaaa acacgcatcg cgcaaaaatg gcttagcagc
    10261 aaagcagcaa aagtcgagct gatccctttg aaaaataatc agcaggctgg tagatacctt
    10321 gacttgaaag taagtattca atatgatgaa tggggctatg atgatatttt gaattttcgt
    10381 ctgccagcag atgttcaaaa acagatcgaa ggagaaaaaa tcgaacttct cgattgggtg
    10441 aatgaaaatc tggaagaaac ttacgttcat gaggcgaatg gggaacgtga agaacaacga
    10501 aaggattttt caagtgaata aatttttagg atttggagca gttgtgatta tgatcgcagc
    10561 aatgggctat gttattttta gcgatgatga ttcgcatgtt caagccggca atcaagcagt
    10621 cgaacgaaca agcaccaaaa caactgctag taaagaaaag aacagtcaaa caaccagcag
    10681 caaggtaaag gacaaagaaa caaaagatcg tacgtccaaa caattgccag atgttaaaac
    10741 agacgattgg aagctagttt tggttggtcc agatcacaaa ttagaaaaag aaattgatga
    10801 gtctactcaa ttggcatcat taagtaacgg atacttgatc gacaaacgca ttgctccaga
    10861 atatgaagca tttgccaagg ctgctgaagc agctggattt ccattagtga tggtctcagc
    10921 ttaccggtct gtatcatccc aacaacaagt tttcgaacaa aatgtccaag atgtgatgag
    10981 tcgtcaaggt gtatcagaag aggaggcgac aaagattact aaagaaacga ttacggagcc
    11041 aggatacagc gaacaccaca caggtctggc agtcgatgtc gtcgatcaaa actggtacaa
    11101 cagttatcct tctactgtat tggatgcaag ttacggagat cagcctggag ctaaatggat
    11161 cgcagataat gctgcgcagt atggatttat cgttcgttat ccaaaaggac acgaagatat
    11221 aactaagatc acatatgaac cttggcactt acgttatgtc ggtaaagaaa acgctgagta
    11281 tatcaccaaa caccaattga cgctagaaga atatctgaat gaattaaatg aaaagtagga
    11341 attgatcatg gcgaaaaaaa agtggaaaaa gcaaaagcgc aagaatatcc gttggccagc
    11401 agtggttgcc ggtgtagcag tgatactctt agcctttgtt ttttccttga agaatctgtc
    11461 ttctcctttt actgataatc agcaagaaaa tgaagcgcaa tcccatcagc aatttattga
    11521 acggcttgtt ccgcatgccc aagaactaca agatggttat ggagtgcttc ctagtatcat
    11581 tttaggacaa gctatattag aatcaaattg gggaaaaagt accttggcta gcaaatacaa
    11641 taatcttttc gggatcaagg cttatgggga tcagaagaaa gtaagcctgg aaacaaagga
    11701 atttgttaac gaagaatgga taactatcca aggagacttc aaagtgtatg attcatggga
    11761 acagtccatg gatgaccata cacaactgtt cgtccaagga gtagattgga atcctgcgct
    11821 gtatgaaaaa gtgattacgg ccaccaacta tcaggaagcc gcacaagcat tgcaagatgc
    11881 aggttatgcg acagaccccg gttatgcgca aaaaatcatc caggtcatcg agacgtatca
    11941 attgaatcag tatgaccgga gatagcaaag gagaagtggc tgatggcaga acaatttacg
    12001 ccagaaatca tgacggaatt gagaaaagat atcgtaagag taccggaaat cattttagaa
    12061 gcaagcggga tcaagatatt tggaaaattg atccgttcga tcatctttac tacggatatt
    12121 gcgatcatac gtaatacaaa tgcgaatgca gtaattgctg tgtatccttt tacacctcat
    12181 ccagcgatta ctaaaagcat tattgaggca gctgacattc cagttttctc cggtgtaggt
    12241 ggtggtttga cacaaggtgc tcgttcttcc tacatgagca tgttcgcaga agcgcaaggt
    12301 tcgttagggg tcgttttgaa cggtcctacg cctttagaga cagtaaaaga agtttgtcga
    12361 gtagttgata ttccagttat cagtacggta acgtcgaaat atacagaaat agatgaaaag
    12421 ttagcagctg gtatttcgat tatcaatatc agtgcaggta aagaaactgc agaagcagta
    12481 cgccattttc gtaaaaaata tcctgaactt cctatcattg cgacaggtgg tccaacggaa
    12541 gaaagtattg ctgagaccat tcaagcagga gcaaatgcca tcacatatac gccaccaagt
    12601 agcggagagc tttttagtcg taaaatggaa aaatatcggg aaactgagaa aaagaatcat
    12661 gaataacagg tgagaatcaa aagacgagga agagcagata gtggctcttc ctcgtctttt
    12721 gtactccagt actatttgtt cagaattcaa aaaaaagaga aatttttttg tttttttacc
    12781 tgtaatgtac ataagaagag ttaaaacctt ttgttattat tagcattcta acaaaaaaat
    12841 gtcttgattt attgaagcgc tttctataga atgaaaaacg aataaaaact aaaaaaggca
    12901 cccgacaatt tagtagcagc taaattgtcg gggcttgaaa agtcagcggg aactgaccaa
    12961 tcaagttgcc atagtcaatg cttgtgcatc gacgtgtata ttgtacctaa tttcatacag
    13021 aattactagt tatttttctg ttttttaggg aagagcgcac acgaagaagc atgcttattt
    13081 agctatgtca aggatattgg gcagttgtct gatatccttt tttctttgcg taattatcct
    13141 cccatcattg aagcaagaag tgtttttagc gatgaaagaa gaatcagcac tatctaattt
    13201 ttcttgaaag tattgagttg ttcatcctag gcagaacgat aaggcaagtc ttggttttta
    13261 ttccccttga aaaagatcgt ttaataaata tgttctgtat aaaaatacag tatattgaaa
    13321 aataacaatg gaagtagagg tgcagagaat gaagaaaaga atgagaatca tcttagcttt
    13381 aagtgttatg ttattcttct tgacggcttg tgggcaagaa gatgacaaaa aaatcacgct
    13441 gaacatggct tggtggggtt cgcaagtccg acacgatgca acagtaaaag taatcgagct
    13501 ttatgaagaa aaaaatcccc atgtgacgat cgattatgaa ttttatgatt atgaaggata
    13561 ttacacaaaa ttaggtacac tagcagcatc agatgatatc tgggacattt tccaactgaa
    13621 caatcaattt cctcaataca tcaatcagat tgaaccatta gatgaatatg tggagcaagg
    13681 gataattgat atttctaatg cagatgaatc ctatttagca acgacgactt acgacgggca
    13741 attagttgga ctgtctaacg gggtgaattc gtttgcgatt gcttacaata aaaatatatt
    13801 tgaacaatta aatatccctg aaccagcttc aaattggaca tgggatgaat ttgaacaaat
    13861 cagctatcaa atcactgaag aattaggaat ctatgctatt tcaagattcg aagattttat
    13921 agctggctgt attattcaaa taccacaagt tgagaaagga ttgaattttt ataatcggga
    13981 agatctatct aacagcttgg gatttgaaaa tccagactat ctaacagatt ttttccagct
    14041 gcgtaaaaat ctggtagaca aaggggccca tccagaacca ggtggtggag ctgcaagtac
    14101 gacggatctg tcttttcagg cggttaggga tagagaagcg gcaatggtgt ttctatcaag
    14161 taaccagttg acagaaatat tgtcaggggc tcctgaagat atggagattc agttgattaa
    14221 tccacctcgc agaaaagcag atggagaatc tggtatccct ttacgttctt cgcaaatgct
    14281 gagtatggca aaaagctcag cccataaaga agaagctgct aagtttattg atttctttca
    14341 aaacagtgaa gaagccaatg aaattttaaa aggagaacgt ggtgtaccaa tcatgtcgaa
    14401 tattcgagaa cagctcgctc gtgcagatga tgcaactttg caagcgacat ttgattattt
    14461 agaaatgatt tccgatatgg acaacggcga agtgaatgtc tttgatagtg ctttgaatcc
    14521 ggaaattcaa gaccaataca atcttttagt tgaaaaagtc atttatgaag aagtaacagc
    14581 agaaggaagg catgaaaaaa gaaaaactaa aacggttctt ggatcaagac aatgtggtcg
    14641 gttacgtatt tgcttctcca ttcatcctag gatttctatt gataacagtg ataccgatga
    14701 tcttgtcact ttattattcg ttcaccgatt atcgtttggg aaatccagtc tcatggatcg
    14761 gcttggataa ttatataaag atgctgagtg atgcccgttt tttgaactcc atccgagtca
    14821 cttttctcta tgtatttgca tctgtacctg tgaaactgat ctttggtcta ttgattgctt
    14881 atcttttaac gaggaatatc aaaggaacga ctttctttcg ttctttgtat tatgtgccta
    14941 gtttgatcgg tggcagtatt tctatcgctc tagtttggcg ggagttgttt tctaaaaacg
    15001 ggttagtgaa tattttcctg gaacgattag gcgcacctgc gctttcctgg tttagcgatc
    15061 aaaaactggt catgatccct cttgttttga tgtctgcttg gcaattcggt tcggcaatga
    15121 tcatctttgc tgcaggtatc aaagagatcc caggctcgta ttatgaagcg gcagagatcg
    15181 atggagcgaa caaatggcag acgttttaca agatcacgtt acctcttctt tcgccgatca
    15241 ttttgtataa cttggttatg cagacaatcg cggcgtttat gacttttacg caggcattta
    15301 tcattaccca aggtggtccg aatgatgcca cgaactttta tgctctttat gtgtataacc
    15361 aagctttttc ttttaattct atggggtatg ctagtgctat ttcgtggatc atgctgttga
    15421 ttatgggagc tgtcaccttt ctgattttca aatcatcaaa aaaatgggtc gttgtgtgaa
    15481 tgagaggagg aagaaatgaa taatacagta acgaaatggt ttcaatacgg ttttattttg
    15541 ctattaggtc tagtcatgat ttatccggtc ttatggatga tcgcaggttc ttttaaaacg
    15601 gatcaagaga ttatgagtgg ttcgctgaat ttgattccag agacttttca atgggaaaat
    15661 tacgcaaaag gatgggcggg gtttgctgga atttcatttt ttactttctt taggaattcg
    15721 tttgtcatct ctgcagtttc gacgataggg acagttctga gctcgacttg tattgcttac
    15781 gcattatcga gaatcgattt caaaggaaaa agattctggt tcgtcgtcat gttagtaacg
    15841 atgatgattc ctgcacaagt gattttgatt ccgcagttta ttatttacaa tcggctgaat
    15901 ttggttggga cgtatgtgcc gctgatttta ccacattttt ttggacaagc atttttcatt
    15961 taccagatca tgcaattcat ggtcagcatc cccaaagagt tagatgaatc agcgattatt
    16021 gacggatgca gcaagtatag tgtctttacc aaaattattt ttcctttgct gaaaccttcg
    16081 atcatcacga cgatcatcat ccagttttat tggaaatggg acgacttcat gggaccattg
    16141 atttatttaa acaagccgag atcttacacg gtatcgattg ccatcaaatt atttgcagac
    16201 gctggatcta gtacgactta cgcgagtatg tttgcgatgt ctacgttgtc gctgctaccg
    16261 gtttttctga ttttcttatt cttcaataaa tatcttgtcc aggggatcag tacaagcggt
    16321 ctgaagggct gaatatgaaa aagccaatag aagagatgtt tctttacaag ctcaactact
    16381 atgtctgtcg gattttttta accagtctta tcagtaatgc tcttttcgct ttagttctag
    16441 gatgtttgtt cactttagga ttagtgctgt ctgctcgtgt tgtctatctg atttattttt
    16501 tgatgatgta tccttcttat tatctatctg tccattactt ggcagaaaaa aaggtatgtt
    16561 ggaggaaagg gaaaatcaaa cggatgtgtt tcatcattac gttgtttcag ctgtttgtca
    16621 ttatttttgc agttgattgg tatttattag tgggaaagat tggagcttgg tatctgattc
    16681 cttttctatt gtgtctgttg ctttttgtat cccatagttt ttttagtacg ctaattgata
    16741 tctgcaaaga agataaagca gatagtcttg tttatttgat caaaaggaat atctggctgt
    16801 tgtcaaaaaa cgtcaagggg tttctgtggt atttatgcct tttgtatgga ctgttctggt
    16861 gtatacagcc tgatttttta ctcactgcat tactgtatcc agggctgcca tttgtcacag
    16921 tattttgctt aaaagaaaag gagaatgact ttaaatgaag aaaaaatatg tacaagtcgg
    16981 acttggtggg agagcgcgtt ttttttatga ggcaattgcc tctacgtatc atcaaacttg
    17041 tgaactagtc ggtttttgtg atgtatctca aacaaggatg gattatgcaa atcggatact
    17101 tgtgaacaag tatggacaga aaaaagtaaa gacatgtatg ccatcacaat ttgagcaact
    17161 actggatgac gtacggccgg ctgaagtcat tattacgtca gtagatcgaa cgcatcatga
    17221 ttatatcatt cgtgcaatga aaaaaggata tgatgttatt tgcgaaaaac ctctgactat
    17281 cgatgaggaa aaagctcaag aaataataac ggtccaaaaa cagacgggaa gaagcctgcg
    17341 agtcactttt aattatcgtt atgcaccgta tcacacgaag ataaaggaac tgatagaaag
    17401 tgatgtgatt ggtgatgtgt tttctatcca cttcgaatgg ctgttaaata cgcaacacgg
    17461 ggcagattat ttcagaagat ggcatcgaaa taagcacaat agcggcgggc ttcttgttca
    17521 taaatctacc catcattttg atctagtgaa tttttggttg aacagccgtc cccaaaaggt
    17581 atttgcattc ggtggattaa acttttatgg gatgcataat gcggagaaac gcggagtaga
    17641 aatattttac agtcgatgct atggaagcga actagctaaa gctgatccat ttgctataga
    17701 cctgtcagcc aatgaggaat tgcgagaaat gtatttagaa gcagaaaaag acagtggata
    17761 tattcgtgac cgcagtgtat tttccgatga tatctctatt gaagatacac taggcgtaac
    17821 ggtcaaatat gaaactggtg cgattttgac gtattcgcta aacgcgtata tgccgtggga
    17881 aggattcaat gtttctttca atggcagcaa aggacggatc gaaatgaaag tgatcgaaaa
    17941 gtcttacatc aatgcaggtg gagataaaaa ggatgaagga gcagccgttc ttcagtccat
    18001 ccatgtctat ccgatgttcg gtgagccata tgaggtaatc gtcgaacaag aagcgggcgg
    18061 acatggcgga ggagatccgc gtttattaga tgatctattt ggtgaagaaa aagaagaaga
    18121 tccgtggaat cgggcagcaa ctcacgtaga tggtattttg tcgattttga caggaatcgc
    18181 agcaaatcat tctattgctt caggaaaagc agtagtgatt gacgagcttg tcagcttaaa
    18241 agaataagaa tagatttacg gaaattactt cccgtagttt ataaaatctt gccttattgc
    18301 cgataggctg tcttttgaac actatttatt cggaaaaagg ggctatgaca tgctttttgt
    18361 catgccttct ttttatgtgg gctgcttttt tagataggag aaaataaaaa taactattta
    18421 aacccgaaca aaaaggaata atgcattaaa aatcattaga ttttacaaaa aaataagtaa
    18481 accacgttgt cagtttcaga aagtagtgtt agactgtttt caatatttga aacaacattt
    18541 tttattcaaa tatggcaaac tgccttcacg tgcaaaacag ttgcaagaaa atgaatttac
    18601 actattatta gttttacata cctacttata tatcgtcata atatggatga cagtttctag
    18661 ccagtaccgt aaatgctgga ctataagtaa aagattggct atttcaccct tcttttctta
    18721 tgataaagtg gagggtgttt ttctttaaca gaaacatagt aggtttttgc acaaaagcgg
    18781 ttatttcggt attaataaat gataaaggag caaataaagt gaaagaatta gttgaacgta
    18841 ttcaaaaaga cggacgtata ttaggcgagg gcgtattaaa agtcgacagc ttcgtcactc
    18901 accaagtaga ccctgtattg atggaacaaa tgggaaaacg gtttgcagaa gtatttaaag
    18961 aacaaggaat cacgaaagtt gtgaccattg aagcttctgg gatcgcacca gctttatttg
    19021 ctgcaagaga attagatgtt ccaatgatct tcgctagaaa agcaaaaagt ttgacgatgg
    19081 atgaagaatt attgacagct tctgtttatt cttttacgaa acaagtgaca agtacgattt
    19141 ctatttctcg taaattttta tcttcagaag acaaagtatt gattatcgac gatttcttgg
    19201 caaacggaca agcggcaaaa ggattgatcg aattatgcca gcaagctgga gcgcaagtag
    19261 aaggaatcgg gatcgtcatc gaaaagtctt tccaagatgg acgtcagtta cttgaagaaa
    19321 tgggattgag agtcgtttct cttgcgcgta ttgcttcctt gacgaatgga caagttgagt
    19381 ttcttgagga ggacgcataa tcgtggaaaa agaaacacaa aatggaaaag ctgcggtttt
    19441 agggctgcag catctgctag cgatgtatgc aggagcagta gcggtgcctc ttttgatcgg
    19501 tacaggacta ggctttaatc aagaacagat gacttatttg atttcaattg atatctttat
    19561 gtgtggatta gcgacattat tgcaattgac agttaaccga tttttcggaa ttggcttgcc
    19621 tgtcgtttta ggatgtgcga tccaagcagt agctccactg atcttgatcg gaaccaatga
    19681 aggagtagga gctatctacg gatctattat cgcttctggg atctttgttg tgttagtttc
    19741 tggtgtcttt tctaaaatta aaaaactatt ccctcctata gtaacaggaa cggttatcac
    19801 cgttattggt ttgactttga ttcctgtagc aatcgagaaa atgggtggag gtaatgcaac
    19861 agcagctgat tttggtgaca aaacaaatct gctacttgca tttgtgacga ttctcctaat
    19921 tgtaggcgtg caaatgctgg caaaagggtt cattcgttca atcgcggtat tgattggttt
    19981 agtcggagga agcatcttag ctgctttcat gggaatgatt gatctgagcg cgattggaga
    20041 tgcgccgttg ttccatgttc cacaaccctt ttattttggt aaaccaactt tcgacgtatg
    20101 gtcgatttta ctaatgatca tcatttccat ggtcagcatg gtagaatcaa caggtgtata
    20161 ttttgcactt ggtgacatta ctgggaaaaa tgttggagaa gaagaattga aaaagggata
    20221 tcgtgcagaa ggacttgccg ttatcttagg cggtattttc aatacattcc cttatactgg
    20281 cttttctcaa aatgtcggat tggttcaact ttcgggaatc aaaacaagaa aacccatcta
    20341 tttctctgca ttcttcttga ttgttttagg attattccct aaaatcggtg cgttagcaca
    20401 aatcatccct gagccggtac ttggtggagg aatgttagtc atgttcggga tggtggcagt
    20461 ccaaggaatc cgtatgctgt tggaagttga tttcagcaat gataaaaatc tattgattgt
    20521 tgctgtttct gttggatttg ggctgggttt caatgttacg ccgacattat tcagtcaaat
    20581 gccgcaaaca ttgcaaatgt tcactggaaa tggcatcgta atgagtagca ttacggctat
    20641 catattgaat ttgatcttta atggattgaa aaaagatgaa gaaatattgg aagaagcctt
    20701 caagaagtaa tagagcaaaa gaaaggttgt gaaatcagcg ttttgacctg attgttagag
    20761 aaacaaacgg aaaaatagtt cctcgtattt tgccgtttgt ttgagctaat accgagggtc
    20821 agctgattga acaccgttta tccataaaaa ggaatgtgac ctaacttttg tcacattcct
    20881 ttttttcatc tttaataaac acgaacaata aaatgttttt ctttgttaac attcgaaatt
    20941 tctttgacat caaaaaaagt gattgttaaa atatgaatgt agaaaaactg ggaggaaaaa
    21001 caatgacagc aaaaatctct gtgattatgg ggagtacttc agattgggaa acaatgaaag
    21061 aagcatgtca aatattggat gaattcggtg tgtcttatga aaaaaaggtc gtttctgctc
    21121 atcgaacccc agatttgatg tttgaatatg cacaaaacgc tcgaaaaaaa ggaatcaaaa
    21181 tcattattgc aggagcaggg ggagccgcgc atctgccggg tatggtcgca tcaaaaacaa
    21241 ctttgccggt tatcggtgta ccggtccaat cacgaacatt aaatggtttg gattcgcttc
    21301 tttcgattgt ccaaatgcct ggaggagtac ctgtagctac tacagcaatc ggaaaagcgg
    21361 gagcaattaa tgctggacta cttgcgatac agatgctttc catgtatgat ttagaattgg
    21421 aggctaaatt ggctgaaaga cgttcaatgt tggctaaaac tgttatagaa agtagtgatc
    21481 aacttggtta aacctttgtt gcctggttca acgatcggga tcgtcggtgg cggtcaatta
    21541 ggtcgtatga tggcgattag tgcaaaagaa atgggcttta aagtaggtgt gctggatcct
    21601 gtgagcgatt gtccagccgc acaagttgca gattggcaca ttgaaagtgt ctatgatgac
    21661 acctttgcgc tgaaagagct tgccagaaga acagatgtca ttacctatga attcgaaaac
    21721 gtcagtgtag aggcgttaaa tgcgatttta cccatgtcat ttattcctca agggacagac
    21781 ttgcttgcga ttacgcaaga ccgcctgtta gaaaaatctt ttttggaaac gaataacatc
    21841 gtgattgctc cttatgcaac gatcgtcagt ccgacggata tccaagatgc gattgacggg
    21901 atcggctatc cttgtgtttt gaaaaccact cgaggcggct atgatggcaa ggggcagtat
    21961 gttttgaaaa gtcgggcgga tctagctcct gccatggacc ttttaagaga aggaacctgt
    22021 gttttagaag catggatccc gtttgaaaaa gagatttcga tcatggtggc aggaaatggt
    22081 caaggagatt tcacgacttt tccggtcgtg gaaaacatcc accacaacaa tattcttcat
    22141 gaaacgattg ctccagccgc gattgatcag gatgtgatcg aggaggctga gcgtattgcc
    22201 cgtgtgattg cagaagcagt gagcctttca ggtgttttag gggtagaaat gtttttaaca
    22261 aaaacaggcg ggctatatgt gaatgaatta gctcctcgtc ctcacaattc tggacattat
    22321 tctattgaag cttgttctat gagtcagttc gatgcccata ttcgaggtat ttgtggttgg
    22381 ccattaggag aaaaagtacg tttgataagc aatgcagtaa tggtcaatgt tttgggagaa
    22441 caactgctgg aaagctatcg cttgattgag aataagccag actggcaatt ccatttctat
    22501 ggaaaaaaac aagcaaaaaa aggtcgcaag atgggacata tcactatctt gacagaagac
    22561 atccagcaaa cgctggaaga aatcaaacaa acgaatattt gggattagag gagaaaaaac
    22621 atgttagaac gttatacacg ccctgaaatg gggaatatct ggacggaaga gaacaaatac
    22681 cgtgcgtggc tcgaagtcga gattttagca gatgaagctt gggcagaact tggtgaaatc
    22741 ccgcaagaag atgtgaaaaa aattcgtgag catgcatcat ttgatatcga tcggatcttg
    22801 gaaatcgaaa aattaacacg ccatgatgtc gttgcgttta cacgagcggt ttctgaaaca
    22861 ttgggagaag aaagaaaatg ggtccattat ggtctaacaa gtacggatgt cgtagatact
    22921 gcttatggct atctcatcaa gcaagcaaat gatattttac gggaagactt gaaacgtttt
    22981 acggaaatca ttggcgaaaa agcaaaagaa cacaaatata cagtgatgat gggtcggaca
    23041 cacggtgtcc atgctgaacc aactactttt ggattgaaac ttgccacatg gtattcagaa
    23101 atgaaacgga acatcgaacg atttgaacat gcggcaaaag gagtagaagc aggaaaaatc
    23161 tcaggagcag tgggaacgtt cgcaaatatc ccgccattta ttgaagagta tgtatgtaaa
    23221 caattgggga tccgtccaca agaaatctct actcaagttc tccctcggga tctacatgct
    23281 gaatatttct cagcgatggc gcttattgct acaagtattg aacgttttgc aacagagata
    23341 cgcggattgc aaaaatccga aacacgtgaa gtagaagaat tttttgcgaa aggtcaaaaa
    23401 ggttcttctg ctatgccgca caaaaggaat ccgatcggtt cagaaaatat ggctggtcta
    23461 gccagagtgg tgcgcggaca catggtcact gcctatgaaa atgtcacatt atggcatgaa
    23521 agagatattt ctcattcttc agcagaacga atcatcttgc ctgataccac gattttattg
    23581 aattacatgt taaatcgttt tggcaatatc gttaaaaatc taacggtctt tcctgaaaac
    23641 atgaagcgga atatgtctgc aacatttggt ttgatttata gccagcgcgt tatgctgaaa
    23701 ttgatcgata agggaatgac aagagaagaa gcttatgatc ttgttcagcc aaaaactgcg
    23761 tacgcttggg acaaccaagt gcagttccgc ccgctgttag aagaagatcc aaccatcaca
    23821 tcggtattat ctaaagaaga aatagacgat gcctttgatt atcatcatca tttgaaaaac
    23881 gttgacgtga tattcaaacg agtaggtttg gaataaagaa gagtgagttg caaaattcaa
    23941 ttaaaataga atagcaaaaa taaccaaaaa agaagcagat ccgttagcgg ttctgcttct
    24001 tttagtttat tatattttct tatcaataat aggaacctga ttttaaaagc ggctacctct
    24061 ttaccgtgcc tctttacata caaaaaatag aggaattaag cgtaaatggc ttggctatta
    24121 taactaaaat gctggttatg gagatgttgc agcaagtttt cgagaaaagc gtattccatc
    24181 tcttgtccat tgaataagat ctggatacga taatgatttt tgaaacgctt ctgttcatga
    24241 atctgactga ttgaaagttc gcctttgtgt tctgtctgat aaagaaaagt ccttttacgg
    24301 cattcagcac agcgcttggt tgctcggcgg atttttcgga atgtataaaa actagtatct
    24361 ttattgcata aataaacgtc tttgatcgac agcaaatcat tatatagaca ggaacgttct
    24421 tcagctgtta attctgataa aaaaagctga ccttgtttta ttgcatcttc catcggcaag
    24481 tgcctcctcg aacccattat ccaaattttt agttaattct aatataccat cattaagtat
    24541 aaatgtaaac gcttgttgat ggtataagat tttcgtttct gataaaacac gaacattaaa
    24601 agaatctaat accttaaatg tacatatttt tgttgattta taaaaagatg attgctaaaa
    24661 tttaaagtga aaaaacgaac aaaaaacgat gtatttgctt ttaatgtaaa taaaaaggag
    24721 agacgtacat ggagaagaaa gaacttttgt atgaaggaaa agccaaaaaa atatatctta
    24781 cttctgatga gaacttgatt tggatagaat atttagatca agcaactgcg ttgaatggcg
    24841 cgaggaaaga ccagataagt ggaaaaggag aattgaataa tcaaatcaca agtgcaattt
    24901 tccgttatct gactcaaaga ggaataaaaa atcattttat tgaacaattg tctaaaaccg
    24961 aacaattagc aaaaaaagta caaattatcc ctctggaagt ggttatccga aatattgcag
    25021 caggaagttt ctcaaaacgt ctaggtgttc ccgaaggtaa agaattcatc aaaccaatca
    25081 ttgaattcta ctataaggat gaccagttgg atgatccatt tatcaatgaa gagcatatct
    25141 atttcttaca acttgcaaaa ccggaagaaa tcagtgagct gaaagcccaa gcgttggcta
    25201 ttaatgaaat tttgaccgaa ttatttgcta aggtcggttt ggttttagta gatttcaagc
    25261 tagaatttgg ccggtcgaat ggacaaattt tgcttgcgga tgaagtaaca ccggatactt
    25321 gccgattatg ggatgaacgt acgaatgaac atatggacaa agacgtttac cgcagagatc
    25381 taggtgattt gatcccagtc tatcaagaag tatataaccg tctatcggaa ctaactgaag
    25441 aaaaggagcg agtatgatgt atttcgtaaa agtatatgta acctataaag attcagtgtt
    25501 agatcctcaa ggggaggcag taaaaaaagc agtccatcgt atgggatatg aaacaatcga
    25561 agatatccga ataggaaaat actttgagat acaacttgct gatgcagaag aaccaatcga
    25621 tacagtgatt gaaacgattt gtgacaagct gcttgcaaat gtaaatatgg aaacataccg
    25681 atatgaaatc aaaaaaatgg aggaagtata acatgaaatt tgcggtcatc gtttttccag
    25741 gttcaaattg tgacgcagat atgttatggg cagttcgcga gatcatgggc gctgatgccg
    25801 aatttgtacg gcatgatgag tcctctttag aaggatttga tggggtttta ctgccgggag
    25861 gattctccta tggtgactat ttgagatgcg gtgcgattgc tcggttttct acaatcatgg
    25921 aagaagtgat tcgttttgct gaagaaggga aaccagtatt cggtacatgc aatggattcc
    25981 agatattgac agaagcagga cttttgccag gtgtactgag acgaaatgag tctttgcatt
    26041 ttatttgcaa aactgtacca ttgaaagtca accccaagac ggcctttact tcagagtatg
    26101 aagccgacga aataatctat cttccagttg ctcatgggga aggaaattat ttctgtgacg
    26161 aagaaacact tcaagaacta aaaaaaaata atcagattgc ctttacttat aatgacaaca
    26221 tcaacggcag cgtggaaaac attgcaggga ttacgaacaa agcagggaat gtgttaggaa
    26281 tgatgcctca tccagaacgt gcagtagaag acttgctagg atcagaagat gggaaacggt
    26341 tctttgcttc cattttaaaa aacttcggaa aggtgaccgt atagatgaaa acattgaaag
    26401 aaccgacagc tgaagagatc aagaaaaaaa gaatctatgc cgaatgggga ttgactgatg
    26461 aagaatatcg aatgatcgaa gaagatatct taggaagaat gcctaattat acggaaactg
    26521 gactgttttc tgttatgtgg agcgaacatt gttcctataa aaattctaaa ccggttctac
    26581 gcaaatttcc tactagtgga cctcatgtgt tgcaaggccc tggagaaggc gcaggcatcg
    26641 tcgacatcgg tgatggacta gcagttgtct tcaaagcaga aagtcacaat catccttcag
    26701 ccgtggagcc ttatgaagga gctgcgacag gagttggcgg aatcatcaga gatattttca
    26761 gcatgggggc tcgtccaatt gcgttgctag attcattgag gttcggcgaa ttagataatc
    26821 caagaacgaa atatttacta gaagaagtag ttgcaggtat cggcggatac ggcaattgta
    26881 tcggcatacc gactgtaggc ggcgaaattg catttgatcc ttgttatgaa ggaaacccat
    26941 tagtcaatgc aatgtgcgta ggtttgattg aacacaaaca tatccagaaa gggcaagccg
    27001 ctggcgtcgg taattcgatc atgtatgtag gtgcaaaaac aggtcgcgac ggtatccacg
    27061 gagcaacttt tgcctcagaa gaatttaatc aagaagaaga acagcaacgt tctgctgtac
    27121 aagtaggcga tccttttatg gaaaagctgc tgcttgaagc gtgtttggaa ttaatcttgc
    27181 atcattcgga ttctctaata ggtatacagg atatgggagc agcaggactt gtttcatcta
    27241 gtgcggaaat ggcttcaaaa gctggctcag gacttatatt gacattagat gaagtcccac
    27301 aaagagaaac tgggatgaca ccttatgaaa tgatgttatc ggaatcacaa gaacggatgt
    27361 tgatttgtgt taaatcagga gaggaagaaa aaatccaaga actatttcag aagtatgatc
    27421 tagatgcagt cactatcggt aaagtaacag acgacgggca atatcggcta tatcatcaag
    27481 gagaagaagt agcaaatctg ccagtcgatg cattagctga agatgcacca gtttatcata
    27541 aagaaatgaa agaaccagct cgaatcgtag aatttcagca gttagctcct tatcagccag
    27601 ttatcgaaga acctggacaa gtattgcttg atctgctgca gcaaccgacg attgcttcaa
    27661 aacgttcaat ctacgaaact tatgattcac aagtacaaac caatacagtt gtccgtccag
    27721 gaagcgatgc tgctgtgatg agggttagag ggacgaataa agcgttagct atgaccacag
    27781 actgcaacgc gcgctatctg tatttggatc caaaaatcgg aggacaaata gcagtggcag
    27841 aagctgctag aaatattatt gctagcggtg gtaagccctt ggcgatcacc gactgcctga
    27901 actatggttc tcctgataaa ccagaagttt tttgggaatt atcgacttca gctgacggaa
    27961 ttgctgctgc gtgtgaaacg ttaggaactc ctgttatttc aggaaatgtc tctttatata
    28021 atgaaacgga tggtcaagca atctatccaa caccaatgat tggtatggtc ggattgattg
    28081 aggatcataa acatatcact actcaggaat tcaaaaaatc aggggatcta atctatatat
    28141 taggtaagac gtttgcagat tttgatggca gtgaattaca aaagatgcag cttggaagaa
    28201 tcgaaggtgt gatccgaaac tttgatctat ccattgaaaa gagaaaccaa gagttagtat
    28261 tgacagccat ccaaaacgga ttgattgaaa gtgctcatga ctgttcagaa ggtggcttgg
    28321 caattgcact ggcagaatca gctttcaaac atcagctagg gatatctgta cagtttgaat
    28381 tgtcctctgc tcaattattt gcagaaactc aatcaagatt tgtgttgaca gtcgcaccgg
    28441 aaaataagac acgattcgaa gaaatgatgg gagatgctgc tgttttagca ggtaaggtga
    28501 cggatgaagc gataatcgaa atttcagcaa ctgacggtca gatcaaaata gaaactgcag
    28561 tagcaagaaa gtgctgggag gatgcaatcg tatgtcttat gaagtgaaga gtttaaatga
    28621 agaatgcggg atcttcggtg tttggggtca tccagacgcc gctagtgtgg catacttcgg
    28681 gctccatagt cttcagcatc gaggacaaga aggtgcaggt atcgtgtcca atgatcgagg
    28741 aaagcttaag gggcatcgag atttggggct gctttctgag gtattcaaag atcaaagaaa
    28801 attgcagcag ttaacaggag aagctgcaat cggacatgta cgttatgcaa cagcaggcaa
    28861 tggaagtgtg gacaatattc agccttttct attcaaattt catgatcagt cgatcggctt
    28921 agcgcataat ggtaacttga tcaatgctcg ttcccttaga aaagagttag aggaaaatgg
    28981 ggcgatcttc cattctaact ccgatacgga gatattgatg catttgatcc gacgaagtga
    29041 agaaacagat tttttaaata aattaaaaag tgctttgaat gaagtcaaag gtggctttgc
    29101 ctatttatta atgacagaaa cggcaatgat tgccgctttg gacccaaatg gttttcgtcc
    29161 attagcaatc ggacaaatga aaaacggagc atacgtgatt gcctcggaaa cttgtgcatt
    29221 agaagttatt ggggcacgtt ttataagaga tgtccagcct ggtgagatag tgatcatcaa
    29281 tgacgaagga atccagattg attgctttac gaaggacacg cagctttcta tttgttcgat
    29341 ggaatatatc tattttgcca gaccagattc aaacatcgca gggatcaatg tgcatactgc
    29401 acggaaaaat atgggacgga atttagcgaa agaatcgcca gtaaaagcag atatggtcgt
    29461 tggcgtacct aattcttctt tgtcagccgc aagcggttat gcggaagcta gtggaatccc
    29521 ttatgaaatc ggattagtaa aaaatcaata cattgcccga acatttatcc agccaacgcc
    29581 tgagctaaga gagcaaggtg ttcgaatgaa attatctgct gtacgaggag tagtggaagg
    29641 taaaaaggtg attatggtcg atgattcaat tgttcggggt acgacaagca gacggatcgt
    29701 ccatcttttg aaagaagcag gggcaaaaga agtccatgtg aggattgctt ctcctccttt
    29761 gaaatatcca tgtttttacg ggattgatat tcaaacgcgt aaagaactca ttgcggcaaa
    29821 tcacacgatt gatgaaatca aagaatgtat cggtgcagat tctttaggtt ttttaagtga
    29881 agagggtctg atcgaatcga tcggtctgga aaaagatgct ccgtattcag gattgtgtat
    29941 ggcttatttc aacggagatt atccgacgcc gttatatgac tatgaagaaa aatatcagga
    30001 atcactaaaa gaaaaagtat cgttctttta aaagcagacc aagaagggag tatatgaatt
    30061 caatggcaaa tgcatacgca aaagctggcg tagatgtcga agctggatat gaagtagtag
    30121 aacggatcaa gaagcatgtc aaaaaaacgg aacgtttagg tatgatgggc gctataggag
    30181 gattcggcgg ttgttttgat ttatcggtct ttcaattaaa agagccagtc cttgtctcgg
    30241 ggaccgatgg cgtggggacg aaattagcag tggcaatcga agcagacaaa cacgatacaa
    30301 tcgggatcga ctgcgtagcg atgtgtgtca atgatatctt agcacaaggc gcagagccgt
    30361 tatatttctt agactactta gcaacgggga aaaatgatcc gttacgtttg gaaaatgtag
    30421 taaaaggtgt agcagatgga tgtgttcaag gaaatatggc gctgattggc ggagaaacag
    30481 ctgaaatgcc tggtatgtac tcaggtaatg attatgatct agccggattt gctgtaggga
    30541 tagctgaaaa atctcaatta ttgacaggag aaacgatcaa cccaggagat gtgttgcttg
    30601 gaatagcatc tagcgggatt cattcaaatg ggtactctct tgttcgcaaa attttctttg
    30661 agcagcagca gttttcttta tcatcaaaac tgccagaatt gaaaaaaaca cttggcgaag
    30721 aattgcttga gccaacgaaa atctatgttt cttccttgct tccgctgatc aaagaaaaga
    30781 aaataaaagg agctgcacat atcactggtg gagggtttgt agaaaatatc ccgcgaatgt
    30841 taccagaatc tatggctgca caaatcaaac ttggctcatg gccgattctt ccaatattcg
    30901 gctgtttaca aaaatatggt gaaattcctg agatggagat gtatgaaatc ttcaatatgg
    30961 gcatcggtat ggtcctggca gtcgaagaat cgaaagtaaa agaaatagta cacgaaattg
    31021 agcaagctgg cgaaaaagtt tatgaaatcg ggaaagtgat cgccaaagaa aaacatcgtg
    31081 tggtgtttat aggagaagaa cgatgagaat cgctgttttt gcttctggaa acggaagcaa
    31141 ctttcaagcg ctagccgatt atctatcgaa aaaaggattg gaatcttcga ttgactggtt
    31201 gttttgtgat cagcctgaag catatgtttt gaaacgtgca actgcgttga gtgtaccggc
    31261 agattgtttt tcacctaaag aatttgattc aaaaaaagag tatgaagaag caattttgca
    31321 taaattgaaa gaaaaaaaga tcgatttgat cgtattagct ggatatatgc gaatcattgg
    31381 accggtattg ctgaaaaatt atgataaaag aatcatcaat atccatccgt ctcttttacc
    31441 tgccttccct ggtttgcatg gcatacgtga tgcctttgaa gctggagtaa aagaaacggg
    31501 tgtcacaatt cattatatcg accaaggtgt agatactgga ccaatcatcc ggcaggaaaa
    31561 agtgagaatt gaacaggagg acacgttcga ttctttagaa gaaaaaatcc atagagtaga
    31621 gcatcgaatc tatccagaag tgattagtga aattattgaa aatggaggct atctgaaatg
    31681 acaagagcat tgatcagtgt atcagataaa actgggattg tagcattcgc ccaaggtttg
    31741 attgaacaag gagtagaaat catttcaaca ggaggaacaa agcatgcgtt ggataaagct
    31801 gggatcaaaa cgctttcgat cgaagaagtg acagggtttc ctgaaatgat ggatggccgt
    31861 gtcaaaacac ttcatcctaa aatccatggt ggtcttttag gaagacgcga tctccctagc
    31921 catatggagg caatggaaac ggaaaatatc caaccaatcg actttgtttg tgtcaattta
    31981 tatcctttca aagaaacgat tatgaaacca gatgtcgaaa tagcagaagc tattgaaaac
    32041 attgatatcg gtggtcctag tatgctccgt agtgcagcaa aaaaccatga atttgtcacc
    32101 tctgttgttg atccatctga ctatgaagaa gtactttcag aattacgtca gtatgggcat
    32161 acttcacttt ctttcagaca aaaactagcg gcaaaagtct ttcgtcatac tgcggcatat
    32221 gatgcactga tcgctcaata cttaacggat tgggtggaag aaaaagaacc ggaaaaattg
    32281 acacttacgt atgaacgaaa acaaacgctt cgttacggtg aaaatagcca tcaagccgct
    32341 gccttttatc agtccgctct tctggaaaaa tattcgatcg cagcagcaaa acaactccat
    32401 gggaaagaac tttcttataa taatatccga gatgcagatg ctgcgctacg gatcatgaag
    32461 gaatatgacg agccaacggt agtcgccttg aaacacatga atccttgtgg tattgggaca
    32521 gcatctacga ttttagaagc gtgggaagca gcgtatgaag cagatcctgt ttcaatcttt
    32581 ggtgggatca tcgttttgaa tcgagaagta gatctgccaa cagcagaaca gatgcataag
    32641 ctgtttctag aaatcatcat tgccccttct tatacacaag aagctttgga attactgcaa
    32701 aaaaagaaaa acatccgatt actgacagta gactttgcgc aggaagaaaa tcaaaatcga
    32761 gaagaaacgg tctcagtctt aggtggactt cttgtacaag atcaagatct ggcaatagaa
    32821 aatgaagaag agtggaaagt cgtgacaaaa cgtaagccga ctccagcaga agcaaaagca
    32881 ttagcgtttg cttggagagc tgtcaaacat gtgaaatcta atgcgatcgt actggcaaac
    32941 gaacgtcaga cagtaggaat cggagccggg cagatgaatc gagtcggttc agtaaaaatt
    33001 gcgattgaac aagcgcaaga gaaaatggaa ggtgcagttt tagctagcga tgcttatttt
    33061 ccaatggatg attcggttga atatgcagcc aaacatggta taaaagcaat cattcagcca
    33121 ggaggaagca tcaaagacca agattctatt gatatggcga ataaatacga tctagcaatg
    33181 gtctttactg atgttcggca ttttagacat tagagaggag tcggataaat gaacattttg
    33241 gtgatcggtg caggtggtcg tgaacatgca ataagtaaaa aacttttagc cagtccgaag
    33301 gtggatacgg tgtattgtgc gccaggaaat ccggggatga caaaagatgg tatcaattgt
    33361 gtcgctatca gtgagcgaga tcattcagcg ttaatccatt ttgttaagaa agaggctatt
    33421 gattggagta tcgttggtcc tgaagttcct ttgttaaatg ggatcgtcga tgattttatg
    33481 gcggaaggat tgaaaatctt cggtccgaca aaagcagcag ctctgatcga aggttcgaaa
    33541 agttttgcta aacaattaat ggatacttat caaatcccga cagctaaatc cagaagtttt
    33601 gaatgttacg aaaaagcagc agcgtatatc aaagaaaatg gtgcgccaat cgtagtgaaa
    33661 gcggacggct tagcagctgg gaaaggtgta gtcgtggcta ccacagtgaa ggaagcgctg
    33721 gatgcggcag aacaaatgtt gaaccagcat cgatttgggg atagtagcaa aaaaatcgtc
    33781 gtagaagagt ttttggcagg agaagaattc tcccttttgg cgtttgtccg taataccaag
    33841 gtgtatccga tggtcatttc gcaagatcat aagcgggcat ttgataacga taaagggcca
    33901 aatacaggcg gaatgggtgc ttattcacca gtaccgcaaa tttcaaaaaa gatgattgaa
    33961 aaagctgtgg aagaagtttt aaaaccagcg gctttgggaa tgaaagaact aggacgctct
    34021 ttcacgggtg ttttatatgc gggtttgatt gctacgaagg aaggaccaaa agtgatcgaa
    34081 ttcaatgcgc gtttcggtga tcctgaaacg caagtcgttc tttctcggtt gaagagtgac
    34141 tttgcgcaag taatcgatga tttattagag aatcgtacag tagagttaaa gtggcagcaa
    34201 gaaggataca atgtaggcgt tgtggttgca gctgccggtt accccgaaga atatgagaca
    34261 ggaatggttc taccagattt tctggaagaa gagctttctg tttactatgc tggagtttcc
    34321 tcaaataacg gcaaactagt tggagcagga ggacgtcttt atttagtaga agcatacggt
    34381 gagacgatca aagaagcaca gaaaaaaatt tacaccgcgt taatggataa aacgacagat
    34441 ggaacatttt atcgtacaga tattggagaa aaagctgcca atagcgaact gatcgtgaag
    34501 taaatcagct gcttccattt ataaaaaata caacaaacat aatcggaagc gccgatttta
    34561 gtgaagcctt ttcataaaga gtgtggtaca cttttgttgt gtatataaat aaggaggagt
    34621 tcagatgaaa aaaattcaat taggtacttc tgatcagaaa gcagctgcag tgatcttagg
    34681 ctgtatgcgt atcaacaatg cggaagatcc agtaaaagtg attgagacag cttatgaaaa
    34741 cggcattgat ttttttgacc atgcagatat ttatggaaac ggtgaatgtg aagaaatctt
    34801 tgcagatgct ctagcaaaaa catcaatcaa acgagaagat ctttttattc aaacaaaatg
    34861 tggaatcgtt ccaggtgtga tgtttgattt ttctaaaaaa catatcattg aatctgtaga
    34921 aaaaagtttg acccgtctga aaatggatta tgtggatgct ttacttcttc atcgtccaga
    34981 tacattggta gaaccagagg aagtagctga agcgtttgat gaattggaac ggtcaggaaa
    35041 agttcgttat tttggcgtca gcaatcaaaa accaatgcaa atcgaattgc tgaaaaaata
    35101 tgttcgtcaa ccattagtag ctaatcagct gcaatttggc ttgaaacata ctggaatgat
    35161 cgatcaaggg attcatgtca atatgacaga tgaagcaagt atcgatcatg acggtagtgt
    35221 acttgattat tcccgattgc atgatatgac gatccaagca tggtcaccat accagtatgg
    35281 attttttgaa ggtgtattta tcgggaacga aaaattccct gaattaaacc aaacattaga
    35341 agaactagca gctaaatatc aaacgacacc aactggcttg gcttctgctt ggattttacg
    35401 ccatccagcc aatatgcaag tgatcgccgg tacgatgaac gtagggcgaa tcaaggaaat
    35461 cgctcaagct tcagaaatta atatgtctcg ggaagattgg tacgaattgt accgtgctgc
    35521 tggaaacatt ttaccttaaa gattgtcata agcaggtcaa aagggctata aattaaaaag
    35581 acagaataac ttgaaagctg ttttcagctt cgagatattc tgtctttttt tattgcaaat
    35641 ggaaagagta tcattgtacg gatttcgttt attagttatt tttgttttta aggaaaagct
    35701 gtgtccgata ttcttttgga gaaatccctt gttttttctt aaataaataa ctgaaatagt
    35761 tagggtcatt ataaccaatt tcaaaagcaa tatctgaaat acgatagtct gattgctgca
    35821 ataatttttt tgctaattcg attctttgtt ctgtcagata atcgatgaat gtttggttaa
    35881 acgattgaga aaaaatcgta ctgaaatgag agggacttaa tgcaacttgt tgagcgaccc
    35941 aagttaagga catatctgga tcggtaaaat tttctttgat aaaatccaat gcatggttca
    36001 aaattggctg atatttgttc cttgaaggct gtgagggcag agcagttaag aaagaaatga
    36061 acatataaac tgtcgaacgg taaagcgagt cgtcggcgct gattgctaag cgttgactgg
    36121 tggatgtgca atgaaccatt tcttgcggga ttttcttttc tttcaggcag acatgctgga
    36181 gtagcttagt caagacaaat aaacgatata atcgtgttcg agttggtgtg ccttgtggtt
    36241 tcgtcaagtg ttcgatgtac ttagatactt cgtttttcga taatgaagac aaatcaagtg
    36301 agaggtcaaa gtaaaagaca gaatcaactg gaagtttcgc ttcttgaatc aaatcctcat
    36361 agctgataat ttgttcttta agggaactgg cggtttcaag tatcatctgc gtgatctcaa
    36421 aagatgcagg taattcgctg attctttctg cgacaggacc aatggcgaca gaaaaattat
    36481 ttttttgtcc cttttctaat tcatggacca acgtttgggc tacctgataa ctggtctcga
    36541 tcacattttc ttcaagagaa tcaactatta aaaatttgat gaatcgagag gaaacagcag
    36601 agaagatcac ttgttgattc tccctaaata aaaacttcaa ataatcactg agatgagcgt
    36661 aatcttcaaa gtctgtactg acttggtttg ctgctaataa tacggtcatt tttttccctg
    36721 ctaaagagcg attgaaagat tcagcaagtt tcaatgtttc actgatcgta agtgagcctt
    36781 tgaacaaacc gttcaagaaa tgattttttt tcaattcgaa aacaagatca gaagaagtgt
    36841 gcgtttcttt ttgctgcaaa tctaaagcag ttactgcttt ttctattgct tgtttcaatt
    36901 cgctactttt aatcggttta agcagataat catctgcctg tacctggatt gctcctttca
    36961 tataatcgaa atcgtcgaat ccactaataa atatgagacg agtccaagga agtagtttcc
    37021 ttgcttcttt tgaaaaagtc aacccgtcca tgaaaggcat ttggatatca gtcagaatga
    37081 tgtcgggctg taagtcgata attgaagaca gtgccatctc accatctcca gcttcgccga
    37141 cataagtgat tggataataa gaaccaagga cagttatttg ttcccgcaag ttattgcgaa
    37201 tgaggtattc gtcttcgatg ataaaaactt tatacatgtt tgtttcctcc aattgttttg
    37261 ggagatagag ggatcatcag tgtgacagtt gttccttctc ggtatttgct gtcaatcgtg
    37321 ataggttttt gatcatttcc gtaataaaga aggagacgct tgtttacgtt gtaaagtccg
    37381 tagccgatac tgaaatctga atcgatccct ttggctaact ctttacgtat ttctgccagt
    37441 ttttctgcag ggatgccaat cccgttgtct ataactttga attggatcat ctcatcaatt
    37501 ttgcatgcgg taatacgaat aaatcctttg tgacggacgt acttcgttcc atggtataca
    37561 gcattttcaa tcaatggctg caagatcatt tttaaaatag aaacaggaaa aagtgtttct
    37621 ggtatatcga tcttatagtc aagcatatgg ccgtatctta tttttaagat gactagataa
    37681 tcattgatat ggctaatttc tttttcaaga ggaatccagt cttgtccttt gcttaaagac
    37741 gtacgaaaaa agccggaaag tgcatgggtc atagtcagag cttcttcaaa gcagtcttgt
    37801 tcgatcaaag aaataatggc atccaaactg ttataaataa aatggggagt aatctgagct
    37861 tgcaatacac gaacttcact ttgtgctaaa tggtattgtt tcaaagcgtt ctcccgtaaa
    37921 gactggttta gatcttccgc cattttattc atgctagcgg taagtgtttg taattccagt
    37981 gtttccggca gggttaaacg aaagccaagg ttccctgaag aaagttgttc tgacatctcg
    38041 atcagctgat tgatcggttc ttggaccttt ctctctaaaa agcgacgatt ctttttcgca
    38101 tagaaaaaaa tggtactaag aactccaagt tccactattg ttaatagaat gagcgagcgg
    38161 acaagtgtcg tatttttttt gcttgctaag tcgatctcca cacgaacaaa atcttgtaag
    38221 atatctgaca gcagttgagt gacagaatct acctgattga tgatctcttc attggtatcc
    38281 ataaaatctt ttgccaagat attattgatc agctcgtttt gataatcttc cagtgtatcg
    38341 atcgtacgta gcgatacatc taacgtacta atttctttaa gtgtatttgt attatttttg
    38401 atctctcgaa tatcattcct aagttcttct aggatatttt cctcattgaa ctcatctacg
    38461 gttacttggc cagaaatcaa gtcccacata ctttccaaaa ctttttgatc aaccttcaaa
    38521 gcaattgcat ctgctttttg gatatttgcg atcgtctgtt gataaatcaa aagctgttgc
    38581 gaatataaca gtatgctcag tagcaaaggg ataacagcaa atagaagcat ggctttgttt
    38641 gcttgggcaa aaatctgttt tatgcttaaa ttttttgtca tgatacttct ccagtcaata
    38701 atttctggtt gggatgttag ctatattctg ctgggaaaag acagtctcgg aaataaaaat
    38761 atctttaggt aatgtttttc ctgtttgaat atttccaaaa taacgagtaa cggtattggc
    38821 tacataccag ccgacatcag gtttacactc gactacacaa ttgacgattc ctgagcgtag
    38881 cttctcgatc atatcttctt gtccatcaat agttacaatg atcaaatctt ctccaggttt
    38941 gattgatgtt tcttttatcg cagctaaagc accgtgagtc atttcatcac tatggctgta
    39001 taatacatcg atttcggata aatcatttgt ttctaaatat cgtttcatct ggtcttttcc
    39061 tttcatccga ataaaatcac cgacaattcg gtgaacaata gtcatcggag gtttatggtc
    39121 gatggttcca tcttttttta gggttggttt acgattgatt gattccaaaa atccttcgga
    39181 gcgtagcttg gtcggtgtgg agttctctga accagcaagt tctaggattc tgatttcctg
    39241 tttctttgaa tttgtgaagt gattgctgac aaataaaccc gcccgatttc cttctgcttt
    39301 gaaactgggg ccaatgtggg tgacgaaaag ctcgggatca ttcgtttgaa tatgtctgtc
    39361 gacaagtatg acagggatcc ctgctcgctt tgcttctttt aatacagagt cccacccgga
    39421 ttcttgcaaa ggagtaaaaa tgatcatatc cactttatag gcaatgaacg tccgtatatc
    39481 ttggatttgc cgttcctgat tcatgtagcc atttcggtag agaacttggt aatcttcttt
    39541 ttcaagcgca gtgataattg attcagtatg ctttttccgc cagctactct ccgttcctga
    39601 ttgcgaaaaa ccaacgacta gtgggtcagg tgcttccttt gtattaggtg tacaccccat
    39661 catttggtaa gcgattacaa caagggctaa aaaaaataaa acacgtttca ctttaaatca
    39721 ctccatctgg atttgttagt actatcttat cagataaaag ggaaaatcaa agtcaaaagg
    39781 aatcaatcga aaaaaatcca agaaacttaa aaaaatccaa gacaaaaaaa gtacacctaa
    39841 aaaatttaaa gtaaatccca taaaaaaatc aatgttaacg ctttctttat tagctatact
    39901 cggtgtatca attgaaatgg gggataaaaa atgaaaaaca aatttagaaa gattgttggg
    39961 actatcgcta ttttgaccgc aagcattcta ttaagtgcgt gtggcaattc aggatctgca
    40021 gatgatagta cggggtatgt aggaatcgct atgccgacca aatcagcaga aagatggatc
    40081 gctgatggga acaacatggt ctcggaatta gaaaaactgg gttataaaac agatcttcaa
    40141 tatggagaag ataaagtaga aaatcaagta gcacaaatag aaaatatgat tacaaaaggg
    40201 gtagatacac ttgtgattgc ttctatcgat ggttcggctt taactgatgt gttagctaaa
    40261 gcaaaagaag cagatatcaa ggtgatcgcc tatgatcgtt tactgatgaa ttcagaaaat
    40321 gtcgattatt acgcgacttt tgataatttt ggagtaggtg tatcgcaagc agcttatatt
    40381 gaagagcact taggattgaa agaaggaaaa ggaccattca cgattgaatt atttggtggc
    40441 tcgcctgacg acaacaacgc actaatcaat tacaacggag tgatgtctgt tttacaacca
    40501 tatatggaca acggtcagtt ggtcgtgcca tctggacaga cgagtttcag tcaaatcgct
    40561 acgcttcgct gggatggttc tacggcgcaa gcaaggatgg ataatctgtt aagcgcaaat
    40621 tatactgacc aaacattgga tgctgttttg tcgccatatg atccaataag tttaggaatc
    40681 atttcgtcat taaaaggcgt ggggtatggg agtgaaagta aacctttacc tgtgattact
    40741 ggacaagatg cgacagttgc tggcgttaaa tcgatcattg ccggggaaca aacacaaacg
    40801 atctttaaag acacacgtat cttagcaaaa aatacgattg aaatgatcaa agcaatcagt
    40861 gatggagaag aggttccggt caatgatact gaaacttatg ataatggggt gaaaactgtc
    40921 cctacgtatt tagcaaatac agtatctgtc gataaagata attatcaagc agagctgatc
    40981 gacaccgatt attataaaga atccgatctg aaaaattaag aggaaccaaa caaggaacta
    41041 aagagatcta agtcagagag aggataagaa ttatggcgga ttgtatttta aaaatggcag
    41101 atatcaccaa aaaattttct ggaatccgag cgttagattg cgtgaatctg atgattgaac
    41161 gtggggaaat ccatgcattg tgcggagaaa acggagcggg aaaatcaaca ttgatgaatg
    41221 tcttgagcgg cttgtatcca tatggctcct atgacgggga aatcttttat gatggaaagc
    41281 tgtgtcaatt taaagatttg aaagatagtg aagaaaaagg catcgtcatc attcatcaag
    41341 aattagcact aagtccttat ctttcaataa aagagaatat ttttctcgga aatgaacaag
    41401 cagaaagagg cgtgattgat tgggaactga cagaaaagaa aacatatgaa ctgctgaata
    41461 aagtcggttt gtctatcgac ccgaatcttc tagtatccca gattggcgta gggcaacagc
    41521 aactagttga aatagctaaa gcattatcaa aatccgtccg tctgttgatt ttggatgagc
    41581 ctaccgctgc attgaatgaa gaagaaagcg cgaatttgct gagattgatc cgagagttga
    41641 aagagcaagg tgtaacctcc atcattattt cacataagct gaatgaaatc gtggatatcg
    41701 cagatcgaat caccatttta cgggatggac agacgattga gacactagaa aatgaaatga
    41761 tcagcgaaga gcatattatt cgcgggatgg tgggcagaga tttagttgat cgttatcctg
    41821 aaagaaaacc ggatattgga gaagtttatt ttaaagtaga agactggacg gtctatcacc
    41881 ctcttgatca cgagcgaatc ataaacgacc acatcaactt gcatttgaaa aaaggagaga
    41941 ttgtaggaat agctggactg atgggagcgg gcagaactga attcgctatg agtctatttg
    42001 gacattctta tggaagcaag atatccggac gtatattaaa ggaaggggca gagatatcag
    42061 ttaagaccgt tccttcagcc attgcaaacg gactcgctta tgtctcggag gaccgaaaat
    42121 ctttaggact gaatctgctg atggatattc gggaaaacgt gacgctgagc agtttggata
    42181 aaataagtca aaaaggagta ttagataaag aaaaagaagt aactgaggca gaaaatttcc
    42241 ggaaaaagat gcgtataaaa acaaactcca tccatcaaat tgtcagtagt cttagtggtg
    42301 ggaatcaaca aaaagtcgtt ttatccaagt ggctgatgac acaacccgat attctttttt
    42361 tggatgagcc aacccgtggg atagatgttg gggcaaaata tgagatctac acgattatcg
    42421 aagaaatggc agcaaatggc aaagctgtct gtcttatctc ttctgaactt cctgaaatat
    42481 tagggatgtg tgatcgaatc tatacgatga acgaaggacg tattacagga gaagtctcgc
    42541 gagaagatgc agatcaggaa gtcttgatga aattaatgac gaaagaagag gtgggttaag
    42601 ttggaacagg caaaaaatct tacggataaa aaagagaaaa aactaaattt gaaaagtaga
    42661 gcagctgatc tttttggtcg ctacagcatg gtgatcattt tagcgggact acttattgcg
    42721 tttcaattga tgacggatgg tattttctgg cgtccattga atataactaa ccttgttctg
    42781 caaaatagcc atatattagt actagctgca gggatgttat tagttgtgct cttaggtcat
    42841 gtggatttgt cggtagggtc agtgatggcg tttgttggtg caattgcggg catgatgatg
    42901 gtcaatcgcc aaatgaaccc gtggatgaca gttatcgcat gtcttgcagt aggcgggctg
    42961 atcggcgcat ggcaaggttt ttgggtagct tatgcaggga taccggcgtt tatcgtgact
    43021 ttagcaggat tgctgatgtt tcgtggactg acacaagttg ttctaggagg ccaatctcta
    43081 gcaccttttc cagctgtttt ccaaaaaatg tctacgggtt atttaccaga cttaagcgaa
    43141 ggaagcagcc ttcacttctt gacattgatt ttaggaatga tcatcacgat cgtgttagtt
    43201 gttggtcaat ggcgaacaag aaatcgtcgg aaaaatcatt tatttgaagt ggaatctcca
    43261 acagctttct tggtaaaggc ggtatttaca gcagtcgttg tactaggaat aacttatatc
    43321 tttgcctctt atcaagggta tccgattatt ttgatcattc ttggtttgat cgttgcagca
    43381 tatgggtttt tgacaaataa aacagtagca ggtcgacaga tttatgcgac gggcggaaat
    43441 cgaaaagcgg ctgaactatc agggatcaaa acaaagcgaa tcacgttctg ggtattcgtc
    43501 aacatgggag tcatggcagc tttagctgga ttagttttag ctgctcgatt gaatgcggca
    43561 acacctcaag caggtacatc gatggaactg gatgcaatgg cggcagtata ttttggtggt
    43621 gcttcgactt ccggcggcat tggaacaatc atgggtgcga ttgtcggtgg cttggtcatg
    43681 ggtgtattga acaatggcat gtcgatttta ggcgtcgggg tagactggca gcaggcaatc
    43741 aaaggattga tcttgctttt agccgttgtg ctagacttgt ataataagaa aaaacgaaca
    43801 acgtaggaga acgccggtta ttcagaaaaa agactgtgac aagacatttg tcacagtctt
    43861 tttttcgcta aaagcgaaca ttcattgttt cgtttgtata gactaaaaat aatatatgtt
    43921 tttttaccat tcgtataaat tctctttttg ctcttgattt ttttgcttaa ttgagttaaa
    43981 ttacataggg cgctaaaaaa agccttgatt aaattaataa tgttcgtgtt ttgctggatg
    44041 tgaacatcag gaggataatg taatgaaagt atttgattac gaagatattc aattaattcc
    44101 taataaatgt atcgtaaata gccgttctga atgtgataca acggtcacat taggtaaaca
    44161 tacattcaaa atgccagtag tgcctgccaa catgcagacg attattgatg aaacaatcgc
    44221 agaattctta gcggaaaatg gttatttcta tatcatgcat cggtttgatg aagcagctcg
    44281 aattccgttt atcaaaaaaa tgaaaaagcg tggattgatt acctcgatca gtgttggcgt
    44341 caagaaagag gaatattcct ttatagagaa gttggcagaa gaatcgctaa atcctgatta
    44401 tatcactatc gacatcgcgc atggacatgc taactcagtt attgatatga ttcagcatat
    44461 taaaaaatat ttgcctgaaa cttttgtgat tgccggtaat gttggtacac cggaagccgt
    44521 ccgtgaattg gaaaatgcgg gcgcagatgc aacaaaagtc gggattggtc caggaaaagt
    44581 atgtattaca aaaatcaaga caggctttgg gactggaggc tggcaactgg cagctcttag
    44641 atggtgtgcg aaagcagctc gcaagccgat catcgctgac ggtgggatca gaacacatgg
    44701 cgatattgca aaatctgtcc gttttggtgc gacaatggtc atgattggtt cattgtttgc
    44761 cggtcacgag gaatctcctg gcgaaacaaa agtcgaaaat ggaatcgtgt ataaagaata
    44821 ctttggttct gcttcagaat tccaaaaagg cgaaaaacga aatgttgaag gaaagaaaat
    44881 ctggatccaa cacaaaggat ctttgaaaga tacactagtg gaaatgcaac aagaccttca
    44941 atcatctatt tcttatgctg gaggaagaga tctggaagcc attcgaaaag ttgactatgt
    45001 catcgtgaaa aattcgattt ttaatggaga cacaatctaa aaaataaata aaatattagg
    45061 atgttgtgaa aaagttgtcc tgcattaagt aataagaaca gccaaacaaa aatagttctt
    45121 tatatgtttt gtttggctgg aattattatc gcagatacaa gcttttgaac atcattattc
    45181 agaataaggg actgtgataa gatttttgtc acagtccctt atgttttgca aaaaaagttt
    45241 ttcttttttc tcgtaaattc ggtaaaatca aaagaaaaaa tagacaatag aaaagagaag
    45301 cttatgtggg aaaaagtaga gaaaaaacaa ggaccgattt atcgtcaaat tatggaacag
    45361 attatgagag aaatcgaaaa cggaaggatc aatcctggaa gtcaacttga atcagaacga
    45421 aaactagggc aactttttgg tgtgaatcgt tccaccgttg tccatgcatt ggaagagctt
    45481 agggagcttg gggtactaac gagcaaacgt ggcagcggat ggtttgttaa tcagactgaa
    45541 tgggggaaat tcgctgttcc aagaatcgat tggcggcaaa tgatctctcc tcgttatgag
    45601 cagacggact ggtatgagca aaaaataaga gaagcaaagc agatccaaaa aacatcgttt
    45661 cttgatttat acgctagcga gatgccagat gagcttttgc cgaattttga gtttccttct
    45721 tattctcttg aagaaatctt agcggaagaa aaagaaatgg atcttttagg ctatactccg
    45781 ttacgtaaca aaatcaaacg gtatttggaa agtaaatttg aattggcatt ttcccccaac
    45841 cagcttcttg tgactggtgg cggtcagcaa gcaatctttt tgattttaca aacgattctt
    45901 tcagctggag aggggatagc tgtagagtca ccttcttttt tttaccgatt acctcttttc
    45961 aaagcttcag gaacgcgttt atttgggata ccgatggatg aagaagggat cgacttagaa
    46021 aaactgaaag aatcgatcca taaaaataaa ttgaaagcag tgcttatcaa cccgaatttt
    46081 caaaacccta caggaaaagt gatgagtcaa aaacgacgag aaaagctagt agaattgtgt
    46141 cgacagtatc ggcttccaat tattgaagac gatgtattta cggatctttc atttctaaac
    46201 caagaagaag tccttcctat acgaacgatt gatccggata atgtgttata cgtcggttcg
    46261 ttatctaggg ttttaggaaa aacaacgaaa atcggttggg tgataggacc tgagactttt
    46321 atctcgcaac tagctcaagc gcaagaaatg atggagtttt cgatgaatat ccttacacaa
    46381 atcacagtgg ctaatgtatt taatgaagca attgatttga aaatgcaaga tgtaagaaaa
    46441 aagttgaaag aaaaaagccg actgataagc gcttggggag attcacaatc gtttttcaag
    46501 cttcattcaa ttttaggtgg ttactatgcc tggattactt gggatgggaa aaaactagac
    46561 cccgaactag cagaaaaagt agtagatatg ggtcttggaa ttgcaccaag tctacttttt
    46621 gggcaagata taaatggaat cagaatcaat tacagtaggc tggatgaaaa ccaaataccg
    46681 ttgttttatt caaagatgaa aattctagaa aagtggttaa cagaataatt tcacaaagta
    46741 aatagattaa agaatataac agcttggatt taaatttcaa gctgtttctt ttttttcgga
    46801 aaaagaatac gatataaaga aactaagaaa aaaccattat ttgtacgtcc gttcacgatg
    46861 tgtaaataaa ataaatgttt gcaacaaaac tgtataacac caacgttttt aaatagaaac
    46921 agatggttgt aagtttgatt agaaaataga aaaaatgcag atgaaatata aaacgctttc
    46981 cttattgtga ttttattcac taatgctgtt aatatgcgga ttggcataat ttcctttcat
    47041 cgtgaaaatt tgtgctttta gcttgtattt ctcataaatc catgataatt tattaatgaa
    47101 gacggttaaa tagtgataca gttttattca ctgtttcaat cgcagaatga agagattttc
    47161 ttatagatga gagaggaatg tgtgacaaat ggcaaacaaa aaagcaccga tcgacttcca
    47221 agcactgcta gatgaagtca atgcagattt cccagtatat caagcacttg atcaagatgg
    47281 aaacgtagtt aacaaagatt tagtacctga tttatccgat gatgaactcg ttgaattaat
    47341 gactcatatg gtatggtctc gtgtattaga ccaacgttca acagcgctga accgccaagg
    47401 acgcctaggc ttttttgcac caactgctgg acaagaggca agtcagctgg ctagtgctta
    47461 cgcatttgat aaagaagatg ttcttcttcc aggttatcgg gatgtacctc aactagtaaa
    47521 acatggttta cctttatctc aagctttttt atggtctcga ggacatgctg caggtaattc
    47581 ttatccggaa gaattgaaag ctctaccacc acaaattatc atcggtgcac aatatgtaca
    47641 agcagccggt gttgcattag gtttgaagaa acgaaacaag aaaaatgtcg tcttcactta
    47701 tactggtgat ggcggttcat cacaaggtga tttttacgaa gcaatcaatt tcgctggcgc
    47761 ttaccatgca aatgccgtat tttatatcca aaacaacggg tatgcgatct ctacacctcg
    47821 tgaaaaacaa actgcagcaa aaacacttgc acaaaaagct gtcgcagctg ggattcctgg
    47881 tattcaagtc gatggaatgg atccgcttgc agtatatact gtatctaaaa tggcacggga
    47941 atgggcgatc agtggaaatg gaccagtcct aatcgaaaca ctgacttatc gttatggacc
    48001 acatacacta tctggtgacg atccaacccg ctaccgtaca aaagacacag atgacgattg
    48061 gcagaaaaaa gatccattga tccgcttccg taaatatttg acggaaaaag gactttggtc
    48121 tgaagaaaaa gaagaacaag tcatcgaagc aactaaagaa gaaatcaaag cagctattgc
    48181 tgaagccgac aaagtaccaa aacaaaaggt ttcagatttc ttgaaaaaca tgttcgaagt
    48241 atctccacaa tcaatcaaag aacaaattgc aatttatgaa gcgaaggagt cgaaataatc
    48301 atggcacaaa aaacaatgat ccaagcaatc acagatgcct tagcacttga actagaaaat
    48361 gacgagaatg tcgttgtttt tggtgaagat gttgggaaaa acggaggagt tttccgggca
    48421 actgaaggat tgcaggaaaa gttcggagaa gaccgagtat tcgatactcc tttagctgaa
    48481 tctggaatcg caggattgag tttcggtctt gcgttagaag gatttcgtcc agttcctgaa
    48541 atccaattct ttggttttat tttcgaagca atggacgaag tagtagctca aatggctcgt
    48601 acaagatatc gaatgagcgg cactagaaat ctacccatca caattcgttc gccatttggt
    48661 ggcggtgttc atacaccaga attgcattcg gataatttag aaggtttgat cgctcagtct
    48721 cctggtattc gagtagtgat tccttccaac ccttatgatg caaaaggact attgatttct
    48781 tctattagaa gtaacgatcc ggttgttttc ctagaacaca tgaaactata tcgctcattc
    48841 cgagaagaag tgccggatga agcttacgaa gttcctttgg ataaagcagc agttactcgt
    48901 gagggaacag atgtatctat tatcacatac ggagcaatgg tgcgtgaagc aatcaaagca
    48961 gcagacaatc ttgcaaaaga aaatatatct gttgaaatca ttgatcttcg tacagttgcg
    49021 ccattagatg tagaaacgat cattcaatcc gtggaaaaaa ctggtcgcgt agttgttgtc
    49081 caagaagcac aacgacaagc gggcgttgcc gcgcaagtag tttctgagat ctcagaacgc
    49141 gctatccttt cattagaagc cccaatcgga cgagtttctg cacctgatac agtcttccca
    49201 tttggacaag cagaaaatgt ctggttgcca aatgcaaaag atatcgaaga caaagtaaaa
    49261 gaaatcgcgg aattttaaga aaagaaggga cgaaacaaag tcatggctta tcaatttaaa
    49321 ttgccggata tcggtgaagg aatcgcagaa ggtgaaatcg tcaaatggtt cgtcaaacca
    49381 ggagatacga tcaatgaaga tgatacatta ctagaagttc aaaatgacaa atcagtagaa
    49441 gaaatcccgt cacctgttac aggaacggtt aaaaatgtga ttgttccaga aggtaccgta
    49501 gcaaatgttg gggatgtatt agtagaaatc gatgcaccag gacacgaaga caatgaaggg
    49561 gacagtggtg tagccgcaga atcccaaact ccagctaaac cggctgctga acccactgtt
    49621 gatacagaat cagctggatc gtcaagcgaa ggagtcttcc aatttaagtt accggatatc
    49681 ggtgaaggaa tcgcagaagg tgaaatcgtc aaatggttcg tcaaaccagg agatacgatc
    49741 aatgaagatg atacattact agaagttcaa aatgacaaat cagtagaaga aattccgtca
    49801 cctgtcacag ggacagttaa aaacgtgatt gttccagaag gcactgtagc aaatgttggg
    49861 gatgtattag tagaaatcga tgctcctgga cacaatagtg catcgtcaac tagtgcgcca
    49921 tcagctgaag caccaaaaga aaaagtggaa acatcaggtt cagcaagtgt cgttgaagct
    49981 gccgatccaa ataaacgtgt attggctatg ccatctgttc gtcaatttgc ccgtgaaaaa
    50041 gatgtagata tctcacaagt aactgcaact ggaaaaggtg gcagagtaac aaaagaggac
    50101 atcgaaaact tcttggcagg tggtccaagc agtgcaccag cgaaatcaga agcgccagaa
    50161 gcggcagcac cgaaagaagc agctcctgca gcagaaagca aaccagctgc accagcaaaa
    50221 ccattcaagt ccaacttagg agacttggaa gaacgtgtag caatgacgcc aacacgtaaa
    50281 gctatcgcta aagcaatggt caacagtaag catacagctc cacacgtgac attgcatgat
    50341 gaagtagaag tgtctaaact atgggataat cggaaacgat ttaaagaagt tgctgctgca
    50401 aatggtacga aacttacttt cttgccatat gtcgtgaaag ctttaacagc gactgttaag
    50461 aaatacccag tattgaatgc atctatcgac gatgcaaacc aagaaatcgt ttataaacat
    50521 tactacaata tcggtattgc aactgatacg gatcacggac tatatgtacc aaatgtgaaa
    50581 gatgcagacc gcaaaggaat gtttgctatt gcagacgaaa tcaatgagaa agcaaaatta
    50641 gctcatgacg gcaaactatc tgcagaagat atgcgcaatg gaacaattac gatcagcaat
    50701 atcggatctg taggtggcgg ttggtttaca ccagtcatca actatccaga agtagctatt
    50761 ttaggtgtag gaacgattgc acaacaacca atcgtaaatg ctgaaggcga aatcgttgtc
    50821 ggtcgtgtga tgaaactgtc attaagtttc gatcaccgta tcgtagatgg agcaactgcg
    50881 caacaagcaa tgaataatat caaacgtctt ttagctgatc cagagctatt aatgatggaa
    50941 ggatgataaa caaatggtag ttggagattt tgctgtagaa ttagatacag ttgtaattgg
    51001 tgcgggacct ggtggctatg tagctgccat ccgcgctgct gaaatgggac aaaaagtagc
    51061 aatcattgaa agagagtata ttggcggtgt ttgtttgaat gtcggctgta ttccttcaaa
    51121 agcactgata gctgcaggac accattatca agagtcacta gattctgaaa tgtttggagt
    51181 aactagtgaa aacgtcaaac tagattttgc aaaaacacaa gaatggaaag aaaacaaagt
    51241 tgtccatacg ttgacttctg gtgttggttt cctattaaaa aaacataagg ttgaaacaat
    51301 cgaaggagaa gcgttcttcg tagatgatca cacactacgc gtcattcatc cagattcagc
    51361 tcaaacttat tcgttcaata acgcaatcat cgcaacaggt tcccgtccaa tcgagattcc
    51421 aggatttaaa ttcggaggac gtgtgttaga ttctactggc ggattagcat tgaaagaagt
    51481 acctaagaaa tttgtgatca tcggtggcgg tgtgattggt gccgaattgg gcggagcgta
    51541 cgcaaactta ggagcagaag taacgattct tgaaggctca ccacaaattt taccaactta
    51601 cgaaaaagat ctagtaaaat tagtagaaga tgatttcaag aaaaaaggcg taacagtcgt
    51661 aacgaatgca atggcgaaag aagcagtcga taacggtgac agcgtgacag tgaaatatgc
    51721 agtggacggg aaagaagaat ccgttactgc tgattatgtg atggtcactg taggtcgtcg
    51781 tccgaataca gatgatatgg gcttagaaca agcgggtgtc gaagtaggag aacgaggatt
    51841 gatcacggtg gataaacaag gacgtacgaa cgtacctaac atctatgcca tcggtgatat
    51901 cgttccaggg gcggcattag cacacaaagc cagttatgaa gcaaaaatcg ctgctgaagc
    51961 aatctctggc aaaaaagtag ctgtagatta taaagcaatg ccggcagtag ccttcactga
    52021 tcctgaattg gcttctgtag gtatgacgat caaagaagca aaagatgctg gtatcgaagc
    52081 aaaagcatat aaattcccat tttcagggaa cggtcgggca ttatcgcttg gtaaaacaga
    52141 aggattcatc cgtctagtta cgacgatcga agataacgtg cttattggcg cgcaaatcgg
    52201 tggagtcggc gcaagtgata tggtctctga attagcttta gcaatcgaat caggaatgaa
    52261 tgctgaagat atcgccttga cgattcatcc tcatccatct cttggcgaaa tcgtcatgga
    52321 tgcttcggaa ttagcattag gtttaccaat ccatatttga tttgaataat acaggcgaaa
    52381 cgcaactgaa aaatcagttg cgttttgttt tttagaatta tcaataatat tattatgtaa
    52441 acttaaaaat atgacaactt cttggtttat taccacttcc tatgctacac tgaagaggaa
    52501 tatgaaatag gagagttgaa gatgcaagaa tatcagtatc cacttgattt agattggaca
    52561 acagacgaaa tggtcattgt aatgaagatg tgggaagatc tggaacgtgc gaatgaaaaa
    52621 gggataaaca atgaagcttt tttgaagact tatcagcagt ttaaaactgt tgtcaaatcc
    52681 attggtgagg aaaaacgatt gggacgggaa tttgaaaaag cttccggtta ttctttatat
    52741 cgtacagtgc agcaagcaaa aaaaaatcca aacaaaactt tgagaatgaa cgggtgagat
    52801 aaatggaaca gatgattgat catatcaagt cctggcttga agaagctggt gagaaaatca
    52861 aatcttctca acaaacatta acaatctcgc aaaaatcaaa tcgaacggat ctagtaacaa
    52921 atgtcgatcg aaacatccag aattttctaa tcgaaaaaat ccattccttt gatccagagg
    52981 cgaaaatatt ggcagaagaa gaagggtgta atcaattatc ggatctcgat ggaagagtct
    53041 ttatcatcga tcctattgat ggcaccttga attttgtgat tgaaggggaa aatttttgta
    53101 ttatgctcgc tgtttatgaa tccggcgttg gaaagcttgg tttcatttat gacgtgatga
    53161 gaaatgaact tttttggggt ggaaaaggaa taggcgtgta taaaaatgat gagccattgc
    53221 ctagaccagc aaataaatcc ttatcggaag gcttacttgg tgtgaatagt tatcttttcg
    53281 gtcataataa atatggaatc cgagaaattg cggaaagcag catggggaca cgtatgtacg
    53341 gatgcgctgg tcttgaacta gtggcaatct tgaaaggaaa ccacattgct tatatttcaa
    53401 accttagccc ttgggattat gcagcgggaa atgtcttatt ggaagagttt ggtatggtaa
    53461 caagcggtct ttttggtact cctttgacat ttgaaaaaag ggaatactat ttagcaggga
    53521 cttcttgtgc atatgcagaa atcgcagacc ttttgaaaaa agaaggctga aaaaaaggta
    53581 gaaatcattc acaaaaatga taaaaacgag caaaagatta attttctcga aaaaatgaaa
    53641 acattttact atatttcatg aaaaaagtct gctataatta gcagtcgagt aaaatttgcc
    53701 ctgtcataaa gaaccaataa aaaaggagtt agttacattg aattatagaa acgatatccg
    53761 taacgtcgca attattgccc acgttgacca cggaaaaaca acattagttg atgaactatt
    53821 aaaacaatca gatacattag acgcacatac gcaattgcaa gaacgtgcga tggactcaaa
    53881 tgcgctagaa aaagaacgtg gtatcacgat tttagccaaa aacacagctg tagactataa
    53941 aggtattcgc gtaaacatca tggatactcc aggacacgcg gacttcggtg gcgaagtaga
    54001 acggatcatg aaaatggttg acggcgttgt gttggtcgta gatgcgtatg aaggaacaat
    54061 gcctcaaaca cgttttgtat tgaaaaaagc actggaacaa catatcacac caatcgttgt
    54121 cgtaaacaag attgacaaac cttctgctcg tcctgagcat gtcgtagatg aagttcttga
    54181 actattcatc gaattaggag cagatgatga tcagttagac tttcctgtga tttatgcatc
    54241 tgcactaaat ggtacgtcaa gcttgtcaga tgatccagca gatcaagaac caaccatggc
    54301 acctattttt gatacaatta ttgaaaaaat ccctgctcct gtcgataaca gcgacgaacc
    54361 attacaattc caagtttcat tacttgacta caacgattat gtaggtcgta tcggaatcgg
    54421 acgtgtattt agaggaacaa tcaaagtagg agaccaagta gcattaatta aattagacgg
    54481 aacagttaag aaattccgtg taacgaaatt atttggtttc tttggactaa aacgtctaga
    54541 gattcaagaa gcaaaagctg gagacttgat tgctgtctca ggtatggaag atatctttgt
    54601 cggtgaaaca gttactcctg tggatcatca agatgcgtta cctatccttc acattgacga
    54661 accaacattg caaatgactt tcttagttaa taattcacca tttgccggtc gagaaggtaa
    54721 attcgtaacc gctcgtaaga ttgaagaacg tttgatggca gaacttcaaa cagatgtatc
    54781 cttacgagtt gaaccaacaa actctccaga tgcatggaca gtttctggtc gtggagaatt
    54841 gcatttgtct atcttgattg aaaacatgcg ccgtgaggga tacgaattgc aagtatctcg
    54901 tccagaagtt attgaaaaag aaatcgacgg tgtaaaatgc gaaccgtttg aacgcgtgca
    54961 aatcgataca ccagaagaat atatgggtag tgttatcgag tccttgagct tacgtaaagg
    55021 tgaaatgcaa gatatggtcc atacaggaaa cggacaaatc cgtttgacat tcttaacacc
    55081 tgcccgcggt ttgattggtt attcaactga attcctttca atgacacgtg gttacggaat
    55141 catgaaccat acttttgacc aatatctgcc aatgcttcct ggtcaaattg gcggacgtca
    55201 ccaaggggcg cttgtttcga tcgatacagg aaaagcaacg acttattcta tcatgagtat
    55261 cgaagaacgc ggtacagtct tcgtagaacc aggtacagaa gtatatgaag gaatgattat
    55321 cggggaaaac agccgtgata atgatttgac tgtcaacatc acgaaagcaa aacaaatgac
    55381 aaacgtacgg tctgctacta aagaccaaac atctgtcatc aaaaaaccaa aacaattgac
    55441 gttggaagaa tctttggaat tcttgaatga tgatgaatat tgcgaagtaa caccagaaag
    55501 cattcgcttg agaaaacaaa ttttagagaa aaatgctcgt gaaaaagcat ctaaaaagaa
    55561 aaaataatct gtaaaaggat gaagagagaa accagcttgt tataaaaaat agctggtttc
    55621 tttttttgtc caactctttt ctgtggagaa gtcatctttg tcaatggaca taaattcaga
    55681 aaatacgccg aaagacgttt aaaagacttt taaaaaactt atttctagag agtttttgtt
    55741 tcataataag ataatgtttc acgaaaggag atcaacttgt ccttcgaaga aaatcgaagt
    55801 aaaatggcaa gagaaaggag cgggaagatg aacgacttat taaaacaata ctttggttat
    55861 gatgagttta gacctggtca aaaagaaatt atccagaaag tcattgatca ggaaaatgta
    55921 ttaggaatca tgcctaccgg aagtgggaaa tcaatctgtt accagcttcc agctttacta
    55981 ttagatgggc ttactgtggt tgtgtcccct ttaatctctt tgatgaagga tcaggtggac
    56041 gcggcgaatc aattaggtat acccgccact tttatcaata gctcgttaga tggttacgaa
    56101 acagctagac gcttccaaga aatcgataga cagcagtacc gtttacttta tatcgctcct
    56161 gaacgtttta tcatgccaga ttttatccaa gcaatgaagc gatggaacgt ccgtatgatc
    56221 gctattgacg aggcacattg tatctctcag tgggggcatg atttccgccc aagttattta
    56281 cagatggcga accagctaga tcaactgcca aatcgtcctg taattgttgc tttgacagct
    56341 acagcgactg tgcaagtagc tgctgacata aaaagacttt taaaaattcc agaaaacaac
    56401 catattcaga ctggttttga acgagaaaat ctacgatttc aagtaattaa agatcaaaaa
    56461 aaagaacaat atctaatcga atatttaaag atcaacaaaa atcaatctgg tatcatttat
    56521 gcagcaacga gaaaagaagt agaccgtctc tatcatttgc ttaaaaaatt cgatttttcc
    56581 gtgggtagat atcatggtgg tctaaatgag aatgagcgaa cagaaatgca agaagccttt
    56641 ttatacgatc gcttgcagct gattgtagcg acaaatgcat ttggtatggg tataaataaa
    56701 agcaatgtcc gttttgttat ccattatcaa attcctggct ctctagaagc atactatcag
    56761 gaagctggtc gggcgggaag agatggtctt tccagtgaag ccatcctgct tttctctcct
    56821 caagatatcc aagtccaaaa gttttttatc cagcagtccc aaagagaaga agggcaaaag
    56881 caaaaagaat atgaaaaatt aaaagcgatg acagaatacg tgtatattga gtcctgtctt
    56941 cagcaataca tcctaaacta ttttggcgag acaagcagtc cgtgcaaccg ttgtggtaac
    57001 tgtttagatg acagagaaat agtagaagta acgacagaag cacagatggt tctttcttgc
    57061 ttaaaacgta tgggtgagaa ctatggtaaa caaatgctga tgaaagtctt agcaggatcg
    57121 aaagaacaaa agctgcaagc tttaggtttt ggacatttat ctacctatgg actgatgaaa
    57181 aaccaatcgc aaaaagaaac gatgcagtta attgaatatt tgatatcgaa tggctatctt
    57241 ctgaccatca atggtgagta tcctgttttg aaagtaacag aaagagggat acaagtattg
    57301 aaaggacaag aatctgttta tcgaaaggaa cccaagaaag tccagcagct atcggatgaa
    57361 gaaaccgata cactatttga agtgctgcgg gaactgcgaa cggatctagc atcagaagca
    57421 ggcgttcccc cgtatgtcgt attttcagat tccacattga aagaaatgag ccgaatacgt
    57481 ccatcaagca gattggagat gcttcagata aaaggagttg gccaaagcaa gcttgataaa
    57541 tatggcgaag cctttttatc tcggataaaa aatgcggact ctaacgttct ggataagtag
    57601 aaagaacgaa tacgtaaaaa tgaataaaaa caactggtta aatctgtttt ttttgctcct
    57661 tatgaaaatc aaatatgtta taattaagga attagaatgg gaggtaagca cagatggatt
    57721 ctttgtcgaa aaaacttgcg atccaagtat taaatgatga agcagaacgg atcaaacgat
    57781 taatccgaaa tcaaaacaac agtctctgta tctcacaatg caaagccttc gaagaagtgg
    57841 tagatacaca gatgtacggc ttttcccggc aagtcggttt tgctgtccgt ataggagtaa
    57901 tcggaaagaa cgagggccag ctgatgttaa gtgagctaga acgagaattg aatcagttat
    57961 atagtgaggt ttaccaagaa atttatgata aaaaagaagt tggcaaggag gaatagactt
    58021 gcctaagaaa gtgaaaaaaa ggcatctatt agactatagc atcttgattc cctatttaat
    58081 tttatgtgta ttgggattga tcatggtata tagttctacg tcgtatctat tattagaaaa
    58141 cggacaaaat ccgtccgcat cagtcattaa ccagagtatt ttttgggtgc ttagcttgat
    58201 tgctatcgca ctactttata aaatgaaaac agatgttttg aaaaaccagc gtttgatcat
    58261 ggcagccatc gcagttttga cgattttgct gctgattgtt gtgttttttg gtaaggagat
    58321 caatggggcg aaaggttggc tgcaaattgc aggattctcg atccaacctg ccgaatacct
    58381 aaaaatcatt tctatctggt acctgtctct gactttatct aaaagacaaa acagtgtaca
    58441 aaaagatttt cttgggactg tgaaacgtcc acttgcaatg gttatcggac ttactgcatt
    58501 agtcgctatt cttcccgatt ttggtaatgc ggcagttatc tttttgatta ttttggttct
    58561 gcttttagcc agcggagtca attatgtgta tactttgatt gtaggagtag gaggcttttg
    58621 tctaagtact tttacgatct ggctgatcaa tatcacaaac ggaaagatct tacccggacg
    58681 tttacagtat atctataatc gatttgctat ttaccaaaat cctttctcag acgaattaaa
    58741 taaagggcat caactagtca acggctacta tgccatgttc aacggaggac tatttggcag
    58801 gggattaggt aacagtatcc agaaaaaagg atttttacaa gaagcacaaa ccgattttat
    58861 ttatgcaatc gtagtggaag aactgggagt gattatgggg attttgatat tagcgttgct
    58921 attctttatg attgtgcgca ttgtccttgt aggtatccgt tcaaaagatc cgttcaattc
    58981 gcttttgtgc attgggatcg gcgcaatgtt tttgatccaa gtatttgtca atcttggagg
    59041 aattactggc gttattcctt tgacagggat cacgttccca tttttaagtc aaggaggctc
    59101 cagtttgctg atgctttcga tttgtgtcgg ctttgtcctc aacatcagtg cggatgaaaa
    59161 acgcaagagc ttaggtttat aataaaaaaa gatgtaggag tggttcgatg aaaaaagttt
    59221 tagttgctaa caggggtgaa atcgctgtac gtgtatttcg tgcttgtaca gaattaggaa
    59281 ttaaaactgt tggaatctat gcagaagaag atgaatattc cgtacaccgt ttcaaagcgg
    59341 atgaagcata tttagtagga caaggaaaaa aaccgattga tgcgtatcta gatattgaag
    59401 ggatcatttc tatcgcgaaa gaatgcggag ctgacgctat tcatccgggt tacggtcttt
    59461 tgtcggaaaa cttgaatttt gcgcaacggt gcgaagaaga aggcatcatt tttgttggac
    59521 ccaaactgca tcatttagat atttttggag ataaaatcaa agcaaaagct gcagctatcg
    59581 aagcggggat tgcttcgatt ccaggcacag atggaccaat cgcttctata gacgatgcct
    59641 tggagtttgc gaaacaatac ggttatccga ttatgatcaa ggcagctcta ggcggcggcg
    59701 gacgcggcat gcgtgtagct cacgatgaaa agagtgcaag agagggctac gaacgtgcga
    59761 aaagcgaagc aaaagcagcc tttggtagtg atgaagtgta tgttgaaaaa tacatcgcta
    59821 atcctaagca tatcgaagta cagattctcg gggatacgca tggaaacgtc atccatcttt
    59881 ttgaaagaga ttgttctgtt cagcgccgtc accaaaaagt agtagaagta gcgccttgtg
    59941 tctctatgaa tgaacagcaa cgtcaaaaaa tctgccaggc agctgtccaa ttgatgaaat
    60001 atgtcgggta tgtcaacgca ggtacagtag aatttcttgt tgaaggagat gatttttact
    60061 ttatcgaagt caaccctcgc gtccaagtgg aacatacgat cacagaaatg attacagata
    60121 ttgatatcgt aacaacgcaa ctgttgattg ctcaaggttt ggatctgcat aaagaaatcg
    60181 gattgcctca gcaagaagga attaaattaa atggttcagc cattcaatgc cgaatcacta
    60241 cagaagaccc gttgaataac tttttaccag acacagggaa aatcgatacg tatcgttcac
    60301 ctggtggttt tggcgtacgt ctggatgtcg gaaatgctta cgcgggctat gtagtgacac
    60361 cgtattttga ttcattgcta gtaaaagtat gtacgcatgg tgctaccttt gaaacagcca
    60421 ttcagaaaat ggaacgttgc ttgaaagaat ttagaatccg tggagtgaaa acaaacattc
    60481 catttatgct caatgtcatc acacatcctg aattccaatc aggaaatgca aaaacaacct
    60541 tcattgatag tacagcaaca ttatttgaat ttcctcgact acgagaccgc ggaaacaaaa
    60601 cgatgaaata tatcggtgaa attactgtga atggttttcc aggtatcgaa agtggcgaaa
    60661 aaccatttta tgaagaacca cgtatgccta aagatctaat cacaagaact gattacgtaa
    60721 cggctaagaa tgtattagat gcgaagggtg ccgatgcttt ggtcgaatgg atcaaaggac
    60781 aggaaaattt gttgttgacg gatacgacat ttagagacgc ccatcaaagt ctgctagcaa
    60841 ctcgtgttcg gacaaaggat ttcaagcaga ttgcacgttt gactggagaa ggtcttcctg
    60901 aactcttttc aagtgagatg tgggggggag caacatttga tgtggcttac cgctttttga
    60961 atgaagatcc ttggcaacgg ttgagaaaaa ttcgttcttt aatgcctaat acgttgctgc
    61021 aaatgctttt cagaggttca aatgccgttg ggtattctaa ttatcctgac aatgtattgg
    61081 tggaatttgt caaagaagca gcagcgcaag gaatcgatgt atttcgaatc tttgatagtt
    61141 tgaactggac tccacaaatg gaaaaaagta tccaagctgt acgggatact ggtaaaatcg
    61201 cagaggcagc tatttgctat actggcgata tcaacgatcc aagtcgggca aaatacaatg
    61261 ttcagtatta taaagacatg gcaaaggaac tagaacaact gggtgcgcat atcattgcaa
    61321 tcaaagatat ggctgggctg cttaaaccgc aggcagctta tcgtttgatt agtgaattga
    61381 aagaaacgac agatctgcca atccatctgc atactcatga taccagcggg aatggaatca
    61441 tcacttattc tgctgcttca aaagcaggag tagacatcgt tgacgtggca atgagtgcaa
    61501 tgagtgggaa tactagtcag cctagcatga gcagcttgta ttatgcactg gtcaatggtc
    61561 cacggctgcc agaaatcacg attgaaaatg cacagaaatt aaaccattat tgggaagatg
    61621 tgcgcatgta ttacaaaccg tttgaaaacg gcttgaatgc tcctgaaaca gaagtatata
    61681 tgcatgaaat gccaggagga cagtattcga atctacagca acaggcaaaa gctgtcggat
    61741 taggtcatcg ctgggatgaa atcaaacaaa tgtatcatac tgtcaacttg atgtttggcg
    61801 atatcgttaa agtaactcca tcatctaaag tagttggaga tatggcacta tttatggtcc
    61861 aaaatgatct tacagaagaa gatatttatg aaaaaggcga aacactaagt ttccctgaat
    61921 ctgttgtgac tttcttccaa ggagaactag ggcaacccgt tggcggattt cctaaaaaat
    61981 tgcaaaagat tattcttaaa ggcagacctg cattgaatga acgtccaggt cttttggctg
    62041 aatcagtcga cttcaacgaa gtaaaaaaag aattggcaga aaaaattggc tatgaaccaa
    62101 aacaagacga agtattaagc tatttgatgt atccgcaagt ctttttggac tatcaaaaag
    62161 catataatca atttggcgat gttacattac tggacacacc aacatttttc caaggtatac
    62221 gtttgggtga aacaatcaat gtacaaatcg aacgcggaaa aacgcttatc attcgtttag
    62281 atgaaatcgg agaacccgat atcgaaggga atcgcgtact gttcttcaat ttgaatggac
    62341 aaagacgtga aatcactatc aaagataatt cgatcatcag tacggtccaa acccgattga
    62401 aagctgaacc gacaaaccga gaacaaattg gagcaacgat gtctggttct gttttggatg
    62461 tattagtgaa aaaaggcgat aacgttaaaa aaggtgatac gttgatgatt actgaagcga
    62521 tgaaaatgga aacggccatc gaagcacgtt ttgacggaga agtcgcacat gtatatgtgt
    62581 cttcaggaga cactatttcg tctggtgacc ttttgattga agtgactgag aaataataag
    62641 cgaaaggtgg ggaaacatga aaagactggg tttgttttgg ctgattctgc tattagttat
    62701 tgtggcattc tacatggaac cagtcttgtt tccgccaaaa cttgaagtga aggaaaactc
    62761 tgatcaacaa atatcaaacc aaatgacaac gtggaaatac caggagctta aagcaactgg
    62821 tttttctact tatatcggaa aaccgatcga tcagctggaa aaggattttg gccaagccta
    62881 tgatcgatta actagcggat ttggttttga aacaagatat tatgaaaata aagctagtca
    62941 tctcacattt gaagcgaaca tccaagacag cagagtcaca actgttaaag tactgcaaac
    63001 agggcctgct gatatcgcgc cgttttattt agggatgacg atgcaagatc ttacgaaaat
    63061 cactactatc tatactaatt tcacttttga gtaccatgat acagaagttg gaatcgaact
    63121 aatggaagag gatatgaatt atcgtccgct aattgctttt gataacgaca cttttgctat
    63181 tttatttttt gatcaaacaa caaacggcct attttcagtt gtgtatttag ataaagatag
    63241 tcttttgaaa ctattgccat atcaagtgtt tggtgaaggc cttccacatt atcaagcaga
    63301 taaatcggct gattgggaat caatcaatcg cgttaaagaa cgaaaaagta cgactttatt
    63361 aaatatgctt agaagagaag acgaacttcc catatacaat caaacacttg cctttcaaaa
    63421 ccacagtcag ctgctactac atgatttttt gacaaggcct gaggaaatat tgtcagagga
    63481 acgtatggct gattggcaaa aatcgctagg cagtgctcaa acgacagtga aattctcact
    63541 gacgaatgat gaattggaga agttaatcag taaacaaaaa ttagaaaata ctagtgggct
    63601 atttattcat ccggcgattg acgctacttt ttcgttttta tattggtaca gcgatcctta
    63661 caaccatgac cgtttcatgg acccgggaac ggattctgta ggcattgctt tttctaaaga
    63721 aaatatgtta gtcttattgc aagaagaaaa cggagaaagt agtgatagca gtgatcgtta
    63781 acgaagagct gtttcaactg gaggatcaat gcctccaagt cgctgaaatg ataaaaaaga
    63841 gtaaaacgat ggcttcttat cttgaacata aacagtcaat ggaagaatct gcaaaagttt
    63901 gccagttgaa agaagcgttc ttgtctaaga aagaagcatt tgaaaatgta gcggcatatg
    63961 gtgagtatgc acctgattat cgtgaaaaaa aacgagctgt tcgaaaagca aaaagacagc
    64021 ttgatatgga tgagcatgtt gcagctttcc gatatagtga aacacagctg caaagtctgt
    64081 tggatcaaat tgtccaagta cttgccgaag aagtttctaa tgaaatcaaa gtagatgcag
    64141 gtagcccgtt ttttgaaacg aaaaaacact ttggatgcgg aggtaattgt catgcaatta
    64201 gacaatagtg aatttgaaat aacaaaacga cgcgggctga tcgtatgggt ctacagctta
    64261 aaacaattaa agaatctgaa aaagttcggc ttgttgcatt atgtctcaag aagaatgaaa
    64321 tatgtcgtgc tttacttaaa tgaagagaca ttggaagaaa cagaagaacg aatccgtaaa
    64381 ttacattttg ttcgaaacgt agaacgttct taccgaccgg atatcgaaat gaattttgcc
    64441 gaaaaaatag gaaacaaaac agtattaaaa gacgaaggtt tcgaaattga agagctaaat
    64501 acagaaatcc gattagctga acatctttaa aaatctctta tgagcagttt ttaaataaaa
    64561 aataacttgg ataacgatcg atttttgatc attgtccaag tatttttttg gtatactagt
    64621 accagtacca tttctaagga gtgagtagat gcggataata tcaggaaatt atggcgggcg
    64681 ccgtttaaaa agtttagcag gggcgaacac cagaccaact acagataaag taaaagaatc
    64741 catattcaat atgattggac catattttga tggagagaca gtattggatc tgtttgcagg
    64801 aagcggtggt ttagctatcg aagcagtatc tagaggctgc agtcatgcgg tatgcgtaga
    64861 caaaaattac caagcgctaa aaatcatcaa agaaaacatc gaaattacga aagaaccaga
    64921 gaaatttacc acaataaaac ttgatgcgga caaagcaatc gaacggctag cagcggagaa
    64981 acaacaattc gactacttgt ttcttgatcc gccatatgca aaacaaaaaa taatcgatca
    65041 aatccataaa atggaagaac tgggactttt ttctaaaacc gcagttatcg tatgcgaaac
    65101 ggataaaaca gtcgaattgc cagaaactgt ggctggattc catcaaataa gaaaacagat
    65161 ttatggcata acggccgtaa ctatctatag aaaagaggga aatcaatgaa acgtgcatta
    65221 ttcccaggaa gttttgatcc ttttacaaaa ggtcacttag atacggtgga acgtgcggct
    65281 aaattattcg atgaggtagt aattggtgtt tttatcaata cttcaaagaa aagtttgttt
    65341 cccccagaag aaagaatgac attgatcaca aaagctgttt ctcatcttcc taatgtaaaa
    65401 gtcatgcatc aagaaaatca attgacggta gaaacagcaa aagagattgg tgcagatgcg
    65461 ttgatcagag gcatacgaag tatcaaagac tttgaatatg aacgagagat tgctcagatg
    65521 aatcatcatc tatatgatga attggaaact gtcttcttat tagcaaaacc agaatatagt
    65581 cacgtgagtt ccagtatttt aaaagaagta ctgcactttg gcggagatgt gagcagctac
    65641 ttaccgccag tgatcaacga agctttagct ggaaagcgtg aatcaaatga aagctaaaca
    65701 atggttgaaa acagtactga ttatcatatt tggaatgttg atcattggat ttgtagtagt
    65761 tcccttgccg tattatgtag aatcaccggg agccacgatc gatctgaaag aactaattac
    65821 ggtcaatgat aaggaagata aaggttctgg gtctttctct cttacctctg ttggtatcag
    65881 gcaagctaca ggtttcttag ctttaaaagc taaaatgacc ccttttgaag aattgatcag
    65941 tgaagaagaa ttgactggtg gggcgacaaa tgaagaatat aatcaaatgc aacagtatta
    66001 tatggattct tcccaaaatg cagcaatcga acaagcgtta aaacttgcag acaagcctta
    66061 taaaatggaa ttcaaaggcg tatatgtcat gagtgtagag aaaacatcga atttctttgg
    66121 caagctttca gttggagata cagtaacaaa agtagacggg aaaagctttc aaagtacaga
    66181 agcatttatg gattatgtaa aaagccaaaa agttggacaa acaatcgaaa tcagttattt
    66241 gcataatggt gaagaaaaaa cagcttctgg tgatctgatc gagttaccaa cagataaaaa
    66301 ggcaggaata ggtatcacgc ttactgacca taccgagatc gaatcagata cagatgttcg
    66361 tattaattct ggctctatcg gtgggccatc agcgggattg atgttcactt tagaaatcta
    66421 tgaacaagtc actggtaaaa acttgcgaca tggtaagcaa attgcaggaa ctggaacgat
    66481 caacagtgaa ggagaagttg gccgaatcgg aggaatcgat aaaaaagtgg caagtgcgga
    66541 taaagctggc gtagagattt tctttgcacc agatgatacg atttcagacg atgttaagaa
    66601 agaatatcct gaaataaaaa ccaactacga agaagcaaaa gctgccgcta aaaaattgaa
    66661 ttcctcaatg aaaatcgtac cagtaaaaac agtacaagaa gcgcttgact atctagagaa
    66721 aatgaaatga aactaaataa ttaaagaggg gttgtgacac gagttctgtc acaacccctc
    66781 tttttcgtca aaaatcttca cgataaaaat aaaaacaaaa gaaaaaattg acgtaattag
    66841 atgttgaagg gacgattttg ccagagctca ctgtttcttc aattttaaat gaaaggagta
    66901 atgaatgatt tttactggtc attctttgaa aaaagaacaa tgatagaaaa aatcagcaag
    66961 aaaatactag cttcacccaa attgatcatt tggatgtcta tgatagttct tatcttttgt
    67021 atcgcaagtg gttttttatt atcaaatttg ctattttata gagaaacaga agagactgct
    67081 gcaaaagaaa tactttcttc gtttagttcc agtggagaag atccatcagc tgaaggaaac
    67141 gatgtagaag aggatgctcg tccaaaggtc atgtacaccg atatcaaagg aagtgtgaag
    67201 gagcctggta tctattcctt ttcttcagaa gaacgggtct atgatgtact aaagcgtgca
    67261 ggtggattat tagaagaagc ggatagcgat cgaatcaact tttctgcgaa gatagaagat
    67321 cagcaagtcc tatatatccc tgctgtaggt gaagagctgc cagaacatct tgatcagttg
    67381 gcatcctcgg aaggaaaaca gcctacagca gattccgagc ctcccaaaat aaatatcaat
    67441 acagccactc catcagaact tcagcagatt cctgggatcg gatcagtcaa agcgcaagaa
    67501 atcattcgat ttagagaaga aaacggatcc ttccagaaag tggaagacct gcaggagatt
    67561 tctggtatcg gtgaaaagac agttgaaaaa ctaaaaaact tcgttacaat taaatagaat
    67621 gattcaaaat cgactggcac gagcaataaa tggacgctaa tggatactaa gaataatcca
    67681 attctaagag cttggcagga cgaggaggta gtatttttgg ataggaaata gcttgattgt
    67741 tcgcaaagaa acattaaaaa aaccaatgct gaatgtctat ctccaaaata aaatatctgg
    67801 gattcatatt atgaatacgg ctgtcagcgg caataacagt caagctctac gagaacgctt
    67861 tgccaaagat gtacttagct atacagcaga taaagtattt attctcattg ggacgaatga
    67921 tctagcagag cataaacaac tttctaaaga aacgtaccaa aaaatctgca gtggatgata
    67981 gttcagctga aaaaagacta tagttctgat catgctttct ttatcacgcc accagcagtc
    68041 gatgagacaa aacaaaaata tcggaataac aagttgatca aagaatatat ctctgttctc
    68101 acagaagtag caaaacgcca tgattgtgaa gtatcgattt ttatacggaa tttcttgaac
    68161 aaatggattc gaagaatttt tataatgggc taatggatga cggacttcat ttcggcagtg
    68221 aagggtagga tcttttgtca gacaggatga ttcgttatat ttaaagaata ttttgtttaa
    68281 gcaatgagta ctttttaatc aacttgaaaa aacggaggaa tataaaacat gttagaaaaa
    68341 tcttcaaaag catgcgatac gaataaagaa aaagttactg ataagtcaca taatgaacgc
    68401 attccttggg accagtactt tatggctcag gcagtactgt tatctttacg tagtacgtgt
    68461 acacgtttag aagtaggagc gacactcgtt cgagaaaaaa ggatcattgc tggcggctat
    68521 aatggagcag tcagtggaga tgtccactgc atagatgaag ggtgttacgt cgtggatgga
    68581 cactgcttgc gaacgatcca tgcagagatg aatgcactcc tacagtgtgc aaaacttggt
    68641 atcccgacgg aaggttcaga aatctatgtc acacattttc catgtttggc ctgtacaaag
    68701 gctttgttgc aagctggtgt gaaagggatc aattatctcc atgactatcg aaatgatcct
    68761 tatgcccaag cgttgatcga acaattaggt gtatctgtac accaagttgc tttagactca
    68821 cagcattttt caaaacttca aaatgaatta acaggaaaac atgtctagcg ttttagaaag
    68881 gcatgttcga aatcggttga tttttccggc aattacgtcg atgattatct gtttgttgta
    68941 ttacactgac aattggggtt atcgtttgct tcttgccgga atttttttat ggttttcgct
    69001 aaaagaagga cgttttatcg cgtacttgtg tattttgtgt agcataacgg tatccatttc
    69061 ttataggcta caagagtatt catttttaga ggaaaaaaca acatctgcgc aaattactgt
    69121 atttacagat acgattcgtg caaatggtaa ttttttgaca atggaaggaa ggttatcaga
    69181 gaataaacaa aagctccagc tttcttatca agtaaacaca gaagcagaat tgaaaacatg
    69241 gcttaacttc cgaggaaagg gaatagaatt gactgctgaa ggcgagttca tctctccagc
    69301 aaaaaagcgg aatctttata attttgacca agaagcttac ttcaggaacc atagaatttc
    69361 tggacggttc caaattcaaa agattgaaca gatcaccatg caaaatcact ggcgaaactg
    69421 gcttcagaga aaaagaggat cttttatctc ctttgtccaa tctgaatttc cgcaaaaaac
    69481 aagtgtatat attaattctt tattatgggg ctataaagat gcaggattca gagaaccaga
    69541 agaaattttt agggagagtg gtttgctgca tctgttcagt ctatcgggtc tacacatcca
    69601 attttattta gggtggctgt attatttatt tcgacgtatc ggatggccac tacacgtcag
    69661 tattattccg ttgagtctac taactatctg ttatgtttct attgcaggaa gttctatttc
    69721 tgttttgcgt gctagtttgc tgtttttatt gcgattgcta gtcaaattgc tgaataagca
    69781 ttattccacc atggacttat ttgcagttgt cttttggctt cttttactat ttgatcctcg
    69841 atttcttttt catgcgggtg gacagcttag ttttttcatg agttttttgt ttatcctgat
    69901 ttcttttgat ggagattggt tgaaaaaaac ggcctctcac gtcctgttta ctatgatcac
    69961 gatgccgttg attgtatggc atttttatga gtggcctatt ttaggtagtc tttttacatt
    70021 actgattgca ccggtattca agtttctttt tcttcctggt gtgttatttc tcacactctt
    70081 caatcgctgg ctgccggaaa ctttactttt gctgatagaa gagggattaa tcttatttga
    70141 gcgaataatc gatttttctt cagggtcgtc tctcattata ggacgtttgc cccttgtgct
    70201 atctgggatg tatattttcg gattactgat ttgtatggat aggttgaaag aacaacctgt
    70261 gaaacacttg ttttatgttg gtcttttttc ttttattttt ttgactttgc catttttacg
    70321 cttttcttca acgatcgctt ttatcgatgt cggtcaagga gacagtatct ttttgcaaag
    70381 tccttttcga aaagaaacga ttttgatcga tactggaggt cagttacact ttgaaagaga
    70441 aaagtgggcg caaggtatga tacgtcctcc tgctgaaaga aatatcgttc catatctaaa
    70501 aggtcagggg ataagtgtaa tcgataaact catcttgaca cacgacgata cggatcatgt
    70561 gggagaatta cccacgttat ctcagcattt tactatcaaa aaaatttata tcggttgggg
    70621 cgctggacga aatgagccat tacaaaggca tttatctgaa gctcaaaaaa atggtactga
    70681 gatcattgaa atcaaacatg gggacaggat cagcggttat tatgatcttt atgtattgac
    70741 tccttttgaa aagggagagg gaagaaatga agattcgatt ggtctatgga tggaatataa
    70801 caataggcgc tttttatttt taggagattt gaatcaagca atggaaaaac aattgctttt
    70861 gatttatcct aatctgaaag ccgatgtagt caaattaggt catcacggta gccgtacgtc
    70921 aagtaacaca gattttcttt cccatattga agcaaaacat gggattatat cctgcggcgt
    70981 aaataatcgt tatggtcacc cgcacagcga agtattggag acactagaag aaaatcagat
    71041 aaaaacatgg cgaacggatc aacaaggaat gatcacctat cgctggcatc cagtttttca
    71101 tcctcatggg ctagtggaaa caatgataga ctagtaattc tcatctgctc atggtaggat
    71161 aatttttgaa agtggtgagt aatttgagta tacaagcaga gttacaaaaa atacgtaaag
    71221 aaaaacttgc atcttgttac ttgattttag gaacagaaaa gtttctccaa gatcaagttc
    71281 ggacagaaat tgtcaaaaaa atccaattag agggcgaaga tgacttaaat tttttaagtt
    71341 ttgacatgga aaatgcaagt ttagatgagg tagtagctga agcagagacg ttgccgtttt
    71401 tcggagatca acggttagta ttcgtagaga atccttattt tttaacaggt gaaaaagtaa
    71461 acaatggaat cgaacaaaat actgatttat tgacggctta tttgaaagaa ccattagaat
    71521 cgactgtatt agtattcttt gcgccatatg aaaagttgga tgaacggaaa aaagtaacga
    71581 aacaattgaa aaaaacagca attatcatca atgtccaaca gttgaatgaa aaagaagtac
    71641 gtcaatatct gatgaatacc ttagagaatt cctcaatcaa aatggaacga agtgcaattg
    71701 atttattctt gaggttgacc gacttagatt tatccaaaat gatgagagag ttacagaaat
    71761 tggtccttta tggacaaaat caacagcaaa tcacggtaag agaagtagaa gaattagttc
    71821 ctaaaacgtt agaacataat atttttgaca tgacccaata tattttaaaa gggaagacgg
    71881 aacaagcttt gcgtttatac gaagatttag ttatgcaagg ggaagaaaca atcaaaatta
    71941 atgcgatttt gctttctcaa ttacgtttat ttttacagac aaaatttctt gtaaagattg
    72001 gttatcagca ggcaaatatt gctgaaacat tgaaaatcca tccatatcgt gtaaaacttg
    72061 ccatgcagga agtaaaaaaa ttcgatgaac atttgctggt acagttgttt gatcaattag
    72121 ttgagatgga ttatcaaatc aaaacgggac aaatcgaaaa agaattaagt tttcaactat
    72181 tcgttttgcg cacagggcag ttgttaagaa atcgataaga agaaagacga gggcaaagca
    72241 tgcgggaagg tcgtttgttt ttgtcctttt tttgacaaat aaaaaaatcg gccgaagccg
    72301 attcttttat ttagcgattt ttttgcttaa acgagatttg tcgcggttag ctttgttttt
    72361 gtggattaga ccttttgatt cagccatatc aatagcttta acagcttctt tatataacgc
    72421 atctacgttg tcagcacctg aagctacagc atcttcaaat tttttgattg cagtacgcat
    72481 agcgcttgtt tgagatgaat ttttcacatt agcgttttcg cttgtacgaa cacgtttgat
    72541 tgcagattca atgtttggca ttagtttcac ctccgataac ttaagttctt acgcgtgtta
    72601 ccgcataatt caacattcat tattatacct aaaggcagcg tgctttgcaa tatgaacatg
    72661 acaagttttt ttccttaaca gtcttttatt aggatttttt ttcagtaaaa acggatagta
    72721 caagctgaaa aaaatcaaaa agacgctatt gtgggaggca atagcgtcaa accaaactag
    72781 atttttattt atctatttaa acaaagtttg aatgggaatg tttggtacta agttattata
    72841 taacaaagag aaccgtttgc aaagtttttt ttgaaaaggg atacgattcc ctgttgttct
    72901 ttgtttttct attaagaaac tgcagttatt ttataaccag tatcctttga tcagtaaagg
    72961 ctattctggt gtaccgatta ttttaatgta ttcatcgata ttttttatca acattttttt
    73021 tatgaccaac tggttccgac ggttccattt gccacttact tccagcacat atttatttag
    73081 gggcaaacgt aagatatatt ttgccaaata gcgatcttta agcaggccat tgatcacacg
    73141 gtcctcaagc tgcaagttga tcccagtcag catcattggc tgttcttgta aaacgtacgt
    73201 atcaattaaa agaccttcaa aagtttccac aagcgtcttc ctttcttttc aaagtaacct
    73261 gaaaacaggc actaattctt ccattttgat attcgttgaa gtggatgcga tcattctttc
    73321 ttttgtccac tgtaatctag tataacaaaa aagagcagaa gagccaaaaa gaaaaaagag
    73381 catcgaattc tataggaagt ctcattggca cggcctaaat tattacaaaa cattgattaa
    73441 aaaaagcccc tgcatcgcag gagcatcccc tatatcgttt taataggtca atcaaaaagc
    73501 agtatcaaac acattttgta tcaaatcagg aaataataga taagctaagc ccagaatcaa
    73561 aagtaccgct agaagaaacc agccgattgt tcctatgaaa tactttaatt tttcgaacat
    73621 tgtatcacct cgttgtcagt atatcacgga tcagtaaatt gggggtgtta agaatctcct
    73681 aaaatagaca aaatttgttc tattttccta aagcaaatac ttgtaagata cagtaagtga
    73741 taccagaagc aatgaccggt ccggcggcga ttcctcttaa gaagacaaca cctaaaatcg
    73801 tgccgaaaac aagtgcaaca gtgatttgtg gatcagaagc aatcaatcct acacccttgg
    73861 aagataagac agccactagc ccaccgcaaa aaacagctac ccagccaata ggggatttaa
    73921 aggcttctaa tagatcacgt agaccgattt ctcctgttgc gatagggatt agaatagccg
    73981 ctgtgatgat cgtcacccca atattgattc ctttgccttg aagccaaggg aaaaaggaac
    74041 gggaggctgg tatgagcttc ataatcaaaa caaaagctgc cgctatcatc agactttgat
    74101 ttttagctaa aattgctaca agtaaaataa ttcctaaaaa aagccaactt tccatgaaat
    74161 atattcctca ctttcttcga aacaatcata acatgttttt agaaagttac aaaagtagga
    74221 attcatcctt caagacctat tcataagctg tttttttcat aaaataaaca aaatcataaa
    74281 aaaacacttt gcttatttga cagaagacag ccgcgctttg tatagtggac aagacttaca
    74341 tttttttgaa gaatcagaaa gatcaggtaa aggtcttagt aaacgcaagc cattcagaat
    74401 aaccaatatt gtgcttcctt cgtgtcctac aactccaaga ggtagattta aaaattgcag
    74461 gaagttagaa gcaatcaaca agagaataac aagaatcgaa aaaataatat tttgtttgat
    74521 gatccgccgc atttttaaag atagccgatg actgtagaca aatttattta agtcgctttt
    74581 cattaagacg atgtctgcga tatccatagc ggcatctgtc ccttgcccca tagcgacacc
    74641 taaagaagcg gctgctaaag caggcgcatc attgactcca tcaccaacca tcgcatttac
    74701 ttggtaaagt tcattctctt ttttgactag ttgtgttttt tcttccggag tacagcttgc
    74761 atagtaatgt tcaatcccta cttgttcagc aatggccttt gctgtgccag aatggtcgcc
    74821 ggttatcata cttgtatgga tgttttggga tttaaagtag gaaattactt gttgggtatt
    74881 tgcttttgga atatctagca gacctaaaac agcaattaac tgctgatctt tagaaagata
    74941 aatcacggtt ttgccttgat tttccaaacg ttcgatcatt tccgcaatct ctttgctgat
    75001 tatcatcgtc tcaggatcat atgcgtgctt gccaactttc cattgcgcac ctaaataggt
    75061 agtttgtaat ccaaaaccag taatttcttc tattggtaag tattggattt cttcaggtat
    75121 tgtgcaatcg aaatattgca aaatcgcatg agcaagtgga tgcgtcgttt tttgttccat
    75181 agcaacaaga agttgttgtg ccaaatcctt ttcatcaaag aaaaagctgt cagttacaac
    75241 tggttttcct ttagtgagcg ttcctgtttt atcaaaggca atcgctttta attcagaaag
    75301 cttttctaaa tggataccgc ctttgattaa aatcccattt tttgcagaat ttgaaagagc
    75361 agctagagaa gctggtgtag cagatgcgac aagcgcacaa ggagaagcca caactaataa
    75421 taccattcct cgataaaagc tttcatccca actccaacct aggaaaaagt gggggagaag
    75481 gatcattaga ggaacagcaa gcaaaatcaa ttttacataa ttattttcga ttcgttcgat
    75541 aaatgaagct gtttgagttg gcgtattctg cgcttcttct actaattgga taatcttagc
    75601 aaagacagtt tcggaactcg tttttgttac tcgaatcgtg atgggatttc cttgattcag
    75661 cgttcctgca tacatctcat cgcctaaata tttttctaca ggcacagatt caccgcttaa
    75721 tgcagactca tcgatcgttg cctgtgtgct ttctaaaaag ccatctatcg gaacagctgc
    75781 tcctttagcg acaaatacca agttatccac ctttagttga gaaacatcta cttcttgcat
    75841 ttgtccattt tcttttaata attgggcttt ttgtggttga agattcatca aagcagtaat
    75901 ttctttttga cttttattag ttgtgtattc ctctaatgca ccgcttaaac aaaagatgaa
    75961 tgtcaacatt gctccttcaa agtaattacc gatcaaacag gcaccaatag cagctaaagc
    76021 catcaataaa tccacattca gtgtccgatc attccacaat tctttcaagc cttccgatgt
    76081 ttgtttatat ccaccgatga taatggcact gatataaaaa agtggggtga tggtaagaga
    76141 gaatcggtcg aagataaaac cgaagatcat aaataaaagg cagccgattg ttgctgcaaa
    76201 agcttttttc tgattagtca tcgttattag acctccttgt caaaacaagg atagcataat
    76261 aaaaatgtaa ttgataataa taacagctaa atgagaacga taatggttat cattt
//
LOCUS       DACDEG010000005        72683 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.005, whole genome shotgun sequence.
ACCESSION   DACDEG010000005 DACDEG010000000
VERSION     DACDEG010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 72683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.005"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..494)
                     /gene="mreD"
                     /locus_tag="IV142_000352"
     CDS             complement(<1..494)
                     /gene="mreD"
                     /locus_tag="IV142_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP8484042.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKK"
     gene            complement(498..1352)
                     /gene="mreC"
                     /locus_tag="IV142_000353"
     CDS             complement(498..1352)
                     /gene="mreC"
                     /locus_tag="IV142_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP8484043.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(1507..2145)
                     /locus_tag="IV142_000354"
     CDS             complement(1507..2145)
                     /locus_tag="IV142_000354"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP8484044.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(2146..2796)
                     /locus_tag="IV142_000355"
     CDS             complement(2146..2796)
                     /locus_tag="IV142_000355"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP8484045.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(2809..3708)
                     /gene="rsgA"
                     /locus_tag="IV142_000356"
     CDS             complement(2809..3708)
                     /gene="rsgA"
                     /locus_tag="IV142_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP8484046.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(3873..5942)
                     /gene="pknB"
                     /locus_tag="IV142_000357"
     CDS             complement(3873..5942)
                     /gene="pknB"
                     /locus_tag="IV142_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP8484047.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(5939..6679)
                     /locus_tag="IV142_000358"
     CDS             complement(5939..6679)
                     /locus_tag="IV142_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP8484048.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(6692..8050)
                     /gene="rsmB"
                     /locus_tag="IV142_000359"
     CDS             complement(6692..8050)
                     /gene="rsmB"
                     /locus_tag="IV142_000359"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP8484049.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(8050..8988)
                     /locus_tag="IV142_000360"
     CDS             complement(8050..8988)
                     /locus_tag="IV142_000360"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP8484050.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(8981..9472)
                     /gene="def"
                     /locus_tag="IV142_000361"
     CDS             complement(8981..9472)
                     /gene="def"
                     /locus_tag="IV142_000361"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP8484051.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(9487..11895)
                     /gene="priA"
                     /locus_tag="IV142_000362"
     CDS             complement(9487..11895)
                     /gene="priA"
                     /locus_tag="IV142_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP8484052.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(12148..12453)
                     /locus_tag="IV142_000363"
     CDS             complement(12148..12453)
                     /locus_tag="IV142_000363"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP8484053.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(12458..13072)
                     /gene="gmk"
                     /locus_tag="IV142_000364"
     CDS             complement(12458..13072)
                     /gene="gmk"
                     /locus_tag="IV142_000364"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP8484054.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(13559..13843)
                     /locus_tag="IV142_000365"
     CDS             complement(13559..13843)
                     /locus_tag="IV142_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484055.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(13861..14751)
                     /locus_tag="IV142_000366"
     CDS             complement(13861..14751)
                     /locus_tag="IV142_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP8484056.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(14771..14989)
                     /locus_tag="IV142_000367"
     CDS             complement(14771..14989)
                     /locus_tag="IV142_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484057.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(14959..16659)
                     /locus_tag="IV142_000368"
     CDS             complement(14959..16659)
                     /locus_tag="IV142_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP8484058.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            16926..17699
                     /locus_tag="IV142_000369"
     CDS             16926..17699
                     /locus_tag="IV142_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484059.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(17780..18349)
                     /gene="thiT"
                     /locus_tag="IV142_000370"
     CDS             complement(17780..18349)
                     /gene="thiT"
                     /locus_tag="IV142_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP8484060.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(18415..18517)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18523..19494)
                     /locus_tag="IV142_000371"
     CDS             complement(18523..19494)
                     /locus_tag="IV142_000371"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP8484061.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(19588..20961)
                     /gene="glmU"
                     /locus_tag="IV142_000372"
     CDS             complement(19588..20961)
                     /gene="glmU"
                     /locus_tag="IV142_000372"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP8484062.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(21464..22282)
                     /gene="purR"
                     /locus_tag="IV142_000373"
     CDS             complement(21464..22282)
                     /gene="purR"
                     /locus_tag="IV142_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP8484063.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(22449..23273)
                     /locus_tag="IV142_000374"
     CDS             complement(22449..23273)
                     /locus_tag="IV142_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8484064.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(23246..23926)
                     /locus_tag="IV142_000375"
     CDS             complement(23246..23926)
                     /locus_tag="IV142_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8484065.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(23949..24887)
                     /locus_tag="IV142_000376"
     CDS             complement(23949..24887)
                     /locus_tag="IV142_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP8484066.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(25132..25983)
                     /locus_tag="IV142_000377"
     CDS             complement(25132..25983)
                     /locus_tag="IV142_000377"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP8484067.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(26137..28710)
                     /gene="mprF"
                     /locus_tag="IV142_000378"
     CDS             complement(26137..28710)
                     /gene="mprF"
                     /locus_tag="IV142_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP8484068.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            28851..29657
                     /gene="proC"
                     /locus_tag="IV142_000379"
     CDS             28851..29657
                     /gene="proC"
                     /locus_tag="IV142_000379"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP8484069.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(29736..31292)
                     /gene="rny"
                     /locus_tag="IV142_000380"
     CDS             complement(29736..31292)
                     /gene="rny"
                     /locus_tag="IV142_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP8484070.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(31703..32752)
                     /gene="recA"
                     /locus_tag="IV142_000381"
     CDS             complement(31703..32752)
                     /gene="recA"
                     /locus_tag="IV142_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP8484071.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(32861..34102)
                     /locus_tag="IV142_000382"
     CDS             complement(32861..34102)
                     /locus_tag="IV142_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP8484072.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(34342..34920)
                     /gene="pgsA"
                     /locus_tag="IV142_000383"
     CDS             complement(34342..34920)
                     /gene="pgsA"
                     /locus_tag="IV142_000383"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP8484073.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(35129..36013)
                     /locus_tag="IV142_000384"
     CDS             complement(35129..36013)
                     /locus_tag="IV142_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8484074.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(36791..38077)
                     /locus_tag="IV142_000385"
     CDS             complement(36791..38077)
                     /locus_tag="IV142_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP8484075.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(38070..39338)
                     /locus_tag="IV142_000386"
     CDS             complement(38070..39338)
                     /locus_tag="IV142_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP8484076.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            39590..40051
                     /gene="mscL"
                     /locus_tag="IV142_000387"
     CDS             39590..40051
                     /gene="mscL"
                     /locus_tag="IV142_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP8484077.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(40110..40556)
                     /locus_tag="IV142_000388"
     CDS             complement(40110..40556)
                     /locus_tag="IV142_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8484078.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(40649..40894)
                     /locus_tag="IV142_000389"
     CDS             complement(40649..40894)
                     /locus_tag="IV142_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484079.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(41009..41587)
                     /locus_tag="IV142_000390"
     CDS             complement(41009..41587)
                     /locus_tag="IV142_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8484080.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(41664..42194)
                     /locus_tag="IV142_000391"
     CDS             complement(41664..42194)
                     /locus_tag="IV142_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP8484081.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(42198..43058)
                     /gene="rlmB"
                     /locus_tag="IV142_000392"
     CDS             complement(42198..43058)
                     /gene="rlmB"
                     /locus_tag="IV142_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP8484082.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(43051..43446)
                     /locus_tag="IV142_000393"
     CDS             complement(43051..43446)
                     /locus_tag="IV142_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP8484083.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(43443..44855)
                     /locus_tag="IV142_000394"
     CDS             complement(43443..44855)
                     /locus_tag="IV142_000394"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP8484084.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(44852..45388)
                     /gene="cysE"
                     /locus_tag="IV142_000395"
     CDS             complement(44852..45388)
                     /gene="cysE"
                     /locus_tag="IV142_000395"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP8484085.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(45710..47170)
                     /locus_tag="IV142_000396"
     CDS             complement(45710..47170)
                     /locus_tag="IV142_000396"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP8484086.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(47340..48497)
                     /locus_tag="IV142_000397"
     CDS             complement(47340..48497)
                     /locus_tag="IV142_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP8484087.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(48531..49901)
                     /gene="radA"
                     /locus_tag="IV142_000398"
     CDS             complement(48531..49901)
                     /gene="radA"
                     /locus_tag="IV142_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP8484088.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(49964..50491)
                     /locus_tag="IV142_000399"
     CDS             complement(49964..50491)
                     /locus_tag="IV142_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP8484089.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            50721..51527
                     /gene="proB"
                     /locus_tag="IV142_000400"
     CDS             50721..51527
                     /gene="proB"
                     /locus_tag="IV142_000400"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP8484090.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            51524..52771
                     /locus_tag="IV142_000401"
     CDS             51524..52771
                     /locus_tag="IV142_000401"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP8484091.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            52847..53191
                     /locus_tag="IV142_000402"
     CDS             52847..53191
                     /locus_tag="IV142_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484092.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(53308..53634)
                     /locus_tag="IV142_000403"
     CDS             complement(53308..53634)
                     /locus_tag="IV142_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484093.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(54250..55452)
                     /locus_tag="IV142_000404"
     CDS             complement(54250..55452)
                     /locus_tag="IV142_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8484094.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            55569..56084
                     /locus_tag="IV142_000405"
     CDS             55569..56084
                     /locus_tag="IV142_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8484095.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(56283..56729)
                     /locus_tag="IV142_000406"
     CDS             complement(56283..56729)
                     /locus_tag="IV142_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP8484096.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(56776..58254)
                     /gene="msr"
                     /locus_tag="IV142_000407"
     CDS             complement(56776..58254)
                     /gene="msr"
                     /locus_tag="IV142_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8484097.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(58608..59237)
                     /locus_tag="IV142_000408"
     CDS             complement(58608..59237)
                     /locus_tag="IV142_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8484098.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(59221..59685)
                     /locus_tag="IV142_000409"
     CDS             complement(59221..59685)
                     /locus_tag="IV142_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8484099.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(60172..61440)
                     /locus_tag="IV142_000410"
     CDS             complement(60172..61440)
                     /locus_tag="IV142_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP8484100.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(61671..62408)
                     /locus_tag="IV142_000411"
     CDS             complement(61671..62408)
                     /locus_tag="IV142_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8484101.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(62475..63044)
                     /locus_tag="IV142_000412"
     CDS             complement(62475..63044)
                     /locus_tag="IV142_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP8484102.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            63172..64044
                     /locus_tag="IV142_000413"
     CDS             63172..64044
                     /locus_tag="IV142_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8484103.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            64386..65258
                     /locus_tag="IV142_000414"
     CDS             64386..65258
                     /locus_tag="IV142_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8484104.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            65255..67213
                     /locus_tag="IV142_000415"
     CDS             65255..67213
                     /locus_tag="IV142_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8484105.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(67545..68396)
                     /locus_tag="IV142_000416"
     CDS             complement(67545..68396)
                     /locus_tag="IV142_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8484106.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            68519..68986
                     /locus_tag="IV142_000417"
     CDS             68519..68986
                     /locus_tag="IV142_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP8484107.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(69249..69731)
                     /locus_tag="IV142_000418"
     CDS             complement(69249..69731)
                     /locus_tag="IV142_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8484108.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(69831..70694)
                     /locus_tag="IV142_000419"
     CDS             complement(69831..70694)
                     /locus_tag="IV142_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8484109.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            71292..72167
                     /locus_tag="IV142_000420"
     CDS             71292..72167
                     /locus_tag="IV142_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484110.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(72312..72464)
                     /locus_tag="IV142_000421"
                     /pseudo
     CDS             complement(72312..72464)
                     /locus_tag="IV142_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
BASE COUNT        20552 a        15563 c        12958 g        23610 t
ORIGIN      
        1 aatttcttaa agggaaaaat gaataataca aatataatca tgttcaacag cagtgtaggt
       61 cctaaaaacc ttgtaataaa atacgtgtta tttactactg ctaatttaaa aatcaactgg
      121 accaccatcg taaacaattc ataacccgtg acaaagatga tcatactgaa aaattcagta
      181 aagatgttga catggataac gtctttcatt acatacatca accatacaat taaagggaga
      241 gctactgcat agataccgat aacgccaata tagtaagcgt catagatagc cccgagaaca
      301 atcgtggtaa tcagtaaata acgtttttca aacgccatac tgcaacataa taatgccaat
      361 atcagaaagt gggcatttga catataggtg cctttcgacc actcccctag cattcttgtt
      421 aaatgaccat ctatcaacat caaaaagaac aaaacgattg gcagatagta cttcattgtt
      481 tctttcttca acatggacta atcctccgct agccgttgga caatcgtcac aactgacacg
      541 tcatacattt ctgcataagg tttcacataa acttcccgat ccagcccata actatctggt
      601 ttcgttttaa taaccgtacc gatcggcaag ttagctggag aattccctcc aagaccggat
      661 gtctggacaa catccccttc tttgatatcc gtatcaccag ttaattgggt caccactaag
      721 gcatggagct tttcatcata gtttttaagc aaaccaaacg cttcgccatt agccgaagat
      781 acccgtactg gaaaatgatt ggagctttca ttagaggatg acagtaattc gattttagac
      841 gaagccgtat tgacctcgat cactcggcca atcaatcctt tttgcgacat gactgccata
      901 tttgcttcga taccgtcttt agaacccttg tctacgatca gcatgtcctg ccaactgtca
      961 ggcgaacgag taatgacatt tgccgttact ttttcgtaac tagtcagcgt ctgattcaac
     1021 tcaagttctt ttttcaacgc ttcgatttct ctctcgtaat tcttgttttg ctgtgaaacg
     1081 tcatcatagc tatcgatttt ttcttttagc cgctcatttt cggcataagt tgaaaaaaga
     1141 ttatggacag aatctactcc atttcgtacc caccgtactg gggcagagat tgttttatca
     1201 accattgcaa cactgtcatt aacgatggat tggaagaaat tgggctttcc ttcattcgct
     1261 cgctgcgcag cggtcacact tagtatcgtc accacgatga taacaataac caaggtaata
     1321 ataatatttt tattaggatt gaacttcttc acaaagacac ctcgaattaa ttagactctt
     1381 gtgttcaatt ttatcactaa atgccgtgaa acaaaaggta agttgctcct cttaagcttt
     1441 tttttagcaa ataatagatt ctgtcatttt tagcctgaaa gacaaatttt tttatttgtt
     1501 tatggatcaa tctttgcttt gaataactgc aatcatacca tctgaaaaag aaaattctgc
     1561 ggtatctgaa agaaattcgt tacttgcata agatgtcgga tattctactt ctttattttc
     1621 caatacatac ttgctgcctt ttaatgtaag attcgagacg ggagtcaagc agcaataagc
     1681 taaatacttc atatctggta tctgtcttac tatatgctct ccaggcaaat aataagacaa
     1741 ataattttga caatctctga taataagctg atgggcaaag ggtttgaagc gaggttccat
     1801 acctaaccag agattggcta aaagatgatc caatcttccg cccgtagctc caatcaaagt
     1861 gacttccgct tgcgggtaac gttcaaatgt ttttaagata cctaactgtg tatctgtatc
     1921 gtctttttct gcaggggaac gggtatactc tttggctttt gcaaatactt ccttctgctc
     1981 ttcgttcgac aatgaatcaa aatcacccac tgccagatct actgggtagc ctgcttgaag
     2041 caggtacagc gcaccacggt ctatcccgac ataggcatcg tatttggaca gcagagactg
     2101 cggccagttt tgcggattac ctccagcagc taataaaatt ttcatttagt ctaatgcctc
     2161 ttttaatcgt tcgatttgtc cgactggatt ttcggctcca tagatatatg atcctgcaac
     2221 gaagacatcc gcccctgcat ctttacagat ttttgctgtt tctggaacga ttccgccatc
     2281 tacttcgatt gcataagtcc aattgttctc tctacgtaga tcagcgagtt ccgtaatttt
     2341 ttctactgtt tcttcaataa agctttgtcc gccgaaccca ggattgacag tcatgactag
     2401 tacttgatca gccaaaggca aaacatgttt gatcatggag actggtgttc ctggattgat
     2461 aactactcca gctttaactc ctaagctttt tgtcatttga agtgcccgat gaatatgagg
     2521 agttgcttcg acgtggacag taatgatatc tgctccagct tttgcaaatg ctggaatgta
     2581 gttttctgga tcaacgatca tcagatggac gtctaacggt aatttagtaa caggacggat
     2641 tgcctgaaca atatttggcc ctaatgtgat atttgggaca aatcggccat ccattacatc
     2701 tacgtggatg taatctgcgc ctcctttttc cactaactca atgtctcttt gtaaattggc
     2761 aaaatctgca ctcaaaatgg atggtgctat cttcattctt gtttcctcct attttcgttt
     2821 accttctttt tttgcataca ttggttttct tttttcgatt tcttgtaaga attgcaaata
     2881 attgtcgtaa cgtgtctgtg cgataccacc tgtttctact ctatgtttta cttcgcagcc
     2941 aggttcttta gcatgcatgc actctctaaa tttgcaatgg acagaagctt ctacaaattc
     3001 aggaaattgt ctcggcagtt ctcctgcttc aattgtcaaa aagtcaatcg agctaaatcc
     3061 tggtgtatct gctactaacc cgccatagat aggaatcagt tccacatgac gtgttgtatg
     3121 ccgtcctctt ccaagtgcat cagaaatctc tcctgttgct aaattcagtt ccggtgatat
     3181 ttgattcagt agcgtcgatt ttcctgctcc agactgaccc ataaagaccg atagccgttc
     3241 tgggaagtaa cggatcagtt ctgtcgtatc acccgtatga ttgggtacaa tcaccggata
     3301 gcctatttta gtataggttt gcttgatctc ttcgattttt tctgggtcgt ctgacaagtc
     3361 ggtctttgtc aaaaaaataa tcggttcgat cgactcgtat tccaaggtaa ctagaaagcg
     3421 gtcaagtaga ttataagaaa atgcaggctc tacgatactt gtgacaatga ttccaagatc
     3481 aacatttgtg actggtgggc gaactaattg attttttctc ggcaagattt ccagaagata
     3541 gccgtctgtt tgattttcac tctcaaagat cacctgatct ccgacaagcg gcgtgatttt
     3601 ccgattccgg aaatttcctc ttcctcttgt ttgataggtc tctccgtttg cgtatacata
     3661 gtaaaaaccg ctgattgctt ttctgatttg tcctttgata agtgccacct ccgtttttgt
     3721 ttattttacc atacgaagag gaaagtaggt gttattccat gcaaacttct caaagtatat
     3781 ggcttgaaaa ttgatcaaaa gagatagaaa cacaaaaaag agaatgcaac aaatcaatgt
     3841 cgcattccct tctgatatat tttattcagc acttattttg aactttctga ggtgctagct
     3901 gatgaacttg tggagctgga agattcttct tttgaagagt tgcttgtgct actggagcta
     3961 cttgagctgc tagaactgtc tgaagagtct ttttcagaac tagaagacgc tggatctggt
     4021 cctttagagt aaattacgtt cacaaccatt cctttggtaa ttgtcgttcc tgcccctggt
     4081 actgtacgaa tcactttatc tttatccacc gtgtttgaat attcggaagt ctcatgcccg
     4141 atatattctg caccgacact agctaaataa ctttgtgctt cgctttttgt atagccggag
     4201 atatcattca atttaggctc tgttccttgg cttacgacaa aattgatgta agtttcttct
     4261 gctacaactt cacccgaggc tggatcctga ctgatgatcg aacctgatgg tacagtgtcg
     4321 ctatagtcat aagtagcact aatattattg atattgaatc cttgtgtaac aagccaatta
     4381 cgaacagttt cgtagttttc tcctacatga ctactcaaag tcactgtttc tggtcctttg
     4441 ctgactgtga aagtaatcgt gtctgtttta ggatcgactt ctgtatcttc tgatggagat
     4501 tgcgaaatga tcattccgct ttctacctct gaactgaatt cctcttcttt ttgaatcaag
     4561 tcagcagaaa accctaattc tttcaaactt gcttcggcat cttcataact tttaccactg
     4621 taatcatcta atttgatttt tttcgtgccg ctactaatat acagagtgac ttcgcgtttt
     4681 tgcttgactg tcgttcccgc tgccgggtct gtcttcacta cattaccttc ttcgattttg
     4741 tcatctgcaa tttcttctgt ttttgttgct acttctaatt tcgcttttgc gagtgtttct
     4801 ctagcagaag cttcagaaag atcagaaaca tcaggaacaa caacttctcc tcgcccgcca
     4861 gaagcaaaga atacagctgt tccgattcct aaaacagcaa gaatagctaa taggatcagc
     4921 caccattttt tctgtccttt ctttttagga tcaggcagaa cttcttcctc ctctggtaag
     4981 gtttcgtttt ctggcttgtc ttttggcaga ggcatagatt tgaatgaatc aggcattggt
     5041 gcatcttccg taaggggtgt gataactttt gtttcattat ccatcacatg aggctgccat
     5101 ttttcttcgt ttgcacgagc tggtgataat actgtagaaa gatcatcaga catttcctcg
     5161 gctgacttat aacggtctgc aggttcttta gccgttgccc gtaaaatcac gttttccagt
     5221 gcttgtggca catttggatc aagtgctttt atagatggaa gatcatcttg aaaatgtttt
     5281 aaagcaattg ttacagcaga ttcaccatca aacggtacgc tacccgtcaa catctcataa
     5341 aggatgatcc ctaaagcata aatatctgac tgcttcgttg ccatacttcc tctggcctgc
     5401 tcaggggaca gataatgaac agatcctagc atcgtattcg tctgcgtgat cgatgtttca
     5461 gataaagcga tagcaatccc gaaatccgtg atttttacaa caccttcatt atcgatcagt
     5521 acgttttggg gtttcaaatc tcggtggatg atttgatggt tatgagctaa agaaatagca
     5581 gaaaggatct gctgcataat atttactgct gtttcataag gaacaggata atgtgtttgg
     5641 atatatcgtt tcaagtccat tcctttgacg tattccatca ccagatactg catgttgtct
     5701 tcttcaccaa catcgtaaac actaacaatg tttggatgaa cgagttctgt tgctgctaaa
     5761 gcttctctct gaaatcgtcg aatcgcagtc tggtcatttt gaaagtcaaa tcgtaagact
     5821 ttaactgcta catcacgatc caagattaaa tcgtgcgcca aaaatacatt tgccatacca
     5881 ccgctaccga tatttccagt gatttgataa cggccattta attttttgcc caactcgatc
     5941 atacactgtc ctccttatat tcgataagaa gaacggtgat attatccgct ccgcctgctt
     6001 cattggcacg atcaatcagc tcagttactt tatgagacaa tgggccttct tgattgatga
     6061 tcgttcctat ttcttcttca gacagcatgt tcgtcaagcc atctgaacat aacagcagtc
     6121 gatcttttag ttgccaaaga tgagtagaaa catctacttc gactgttcca ggcatgccga
     6181 cagaacgtgt taaaatattt ttccttggat ggttcactgc catttcttcg ctgatttctc
     6241 ctgatttgac tagttcattg actaaagaat gatcctccgt caattgcatg attttatctt
     6301 cacgaatcaa ataagctctg ctatccccaa cattggcaat cgtgaaccag tcatctgaca
     6361 gagcggccgc tacgattgtt gttcccatac cgttatattc cggctgttct tgtccacgct
     6421 gataaatcag catattttct tcttgaatcg attgaatgaa ccattgggcg attttttcat
     6481 cgtcaacagc ttcttgtttt tcccaagctt cgcctagatt cgttaccgcc atctgactgg
     6541 caatgtctcc ggcacgatga ccacccatcc cgtctgctaa tacagcaagc ttgagacctg
     6601 aacggctggt aaatacactg gcatagtctt gatttgtgtt acgtcgtctt cctacatctg
     6661 attgatattc aatctgcatt ttttcacctc attacttttt gcgcaaacaa caaatgaaaa
     6721 atccatctgt gtaaaactga tgcggataca aagtcaacat tttctcttcg attgatgatt
     6781 gtagcatagg attggtcaaa acatcaatcc tctcaaaatc agggtgtctt tccaagaaag
     6841 cagaaacaac gtcttggttt tcttctttta agatcgtaca agtgctataa gtcagaatac
     6901 cggaagactt taaagttggt gcgcagcttt ctaaaatttc caattggatt tttggtaaat
     6961 tcgctaattc atccggctgt tttttataac gaatatctgg ttttcgccga agcagcccaa
     7021 gacctgaaca aggcgcgtct actaaaatcc gatcaaactg ctctgcttcg aactgtttac
     7081 tgacttctct agcatccatc ttttgagcgt agacagcaga agagacacct agacgtgctg
     7141 cattatcttc aatgagtttg actttgtgtt cgtgtacatc taaagcgacc acttctccac
     7201 cgcatgacgg atctaaaaaa gaagcaatat gcgtcgtctt acctccgggt gctgcacagg
     7261 catctaagac atgatgttca ggctcgattt gcatagacgg cgctacaagc atcgaacttt
     7321 catcttggat cgtcatgacg ccatcgtgga acaatgagct agaagctaaa tgcccttttt
     7381 tcgctactat accgtatgga ctgacttggc ttttttcagc tgtgatctct tcttcttgca
     7441 aaacagtgat ggcctgatct ctggaaagtc ggcgtgtatc tactcgggca ctagcatggc
     7501 taggctgata tagcgataaa ccgagctttt cagtttcttc gtatccgatt tgatccacca
     7561 gtcgtttcgt catccataga ggcaggctga ttttgacaga aagtctttcg ataggatcag
     7621 caatgccttg cagatctggt gctccttgac gctgataatt cctcaagaca ccatttacaa
     7681 atttccctgt cccaggattt ccttttactt ttgcgatttc gaccgcctcg tttaaaatag
     7741 catgtgcagg gactttatct aaaaattcta attggtatac agaaagataa agaagcatct
     7801 tgacccagtc atccactttt tttgctttag cgatcaatgg tgcaacgtaa aattccaata
     7861 gtcgctgatg gctaatcgta ccatagatca attctgtaaa taaacccaca tctttttgat
     7921 ttagctcgct ttgtttgatc atctcattca acaaaagatt cgagtaggcg ccacccttgt
     7981 tcactcgctc taatgtcagt aatgccatat atcgtactga ttttttcatt tttttaggga
     8041 tttttttcat tatttcacct gctgaccaat ctgaacttgc tgaccgcttc cgttaaggaa
     8101 atcatgtacg gcctgttttc ctttaccagc tggctgtaat tcattgatgg ctaagacggt
     8161 ctgtttaccg caagcaatcc atagattttt tttgtctttc cggatgatcg taccaggtgc
     8221 tttgtctgta gtttctgcca atacttcaac cgataataat ttccagcgta tgccctcata
     8281 ggtcgtgaat gcaattggcc aaggacgcat tccgcgtact tggttgtcaa tttcttctgc
     8341 agttttatgc caatcgattg cttcttgttc ccgtgtgatg tttggcgaaa aagttgcttc
     8401 tgactcgtct tgaggtactg gtttttgtcc atccaaaata ttgggcaatg tttctagcaa
     8461 tagctttctt cctaaaaggc taagcttttc aaacatcgtc cctacatcat cttgtttagt
     8521 gattggtatg ctttcttgcg catagattcc gcctgcatcc atttttttga tcatttccat
     8581 gatcgttaca ccagtttctt tctccccatt catgatggca taatgaacag gcgcacctcc
     8641 ccgatattta ggaagtaaag aagcatggac attgattgcc cctaattttg gtacttgcaa
     8701 taacttttca ggcaagaatt ggccaaatgc cgcagtgacg atcacatcag gcaccaattc
     8761 aatgatttct tccatttctg gcgaaccgga gattttctct ggctgcagca cacggatacc
     8821 gtgcttcaga gccgcttttt ttaccggtgt cggcgtaatt actcgtttcc gcccaactgg
     8881 acggtcgggc tgtgttacca ctgcctggat ctcgtaacct gattctatca gcccttcaag
     8941 aatcggcaca gaaaaagcag gtgtgcccat aaatactagc ttagtcatca agatgttcct
     9001 ccatatattc ttcaagttct tcttccggga tctgctgtat cattttctca atgaacaaga
     9061 taccgtctaa gtgatcgatt tcatgctgga aggcacgggc taaatagcca aaagccgtca
     9121 cttctatctc ttctccgtct cgatcataat aacgcacggt cacttcatct gctcgcttga
     9181 ccgttccata gacatgcgga atactgagac aaccttctac gtcaaggctt tcaccttttg
     9241 cttcgatgat ttctgggtta atcagttcaa atttctcgcc ttcatctact tcgatcacag
     9301 caattctctt gttttgtcct acttgaggag cagcgatccc tattccgtca ttcgcaatca
     9361 ttgtctcata taaattgtct aataaagcga ttgtctcgtc cgtgattaca tcgattggct
     9421 gagcagttct ttttaattta tcattagggt ggatcaatat aggatagcgc ataatttggc
     9481 cctttcttaa ataaagttta atggttcgtt gtcaattgcc acaaaaagac cttgtcgctg
     9541 ttctttctgg cttccattca aaatttcatc caaaacatga ggaagattcg gttcatgttt
     9601 atatttgatg attagttgat aataatatcg gtttttcact ctcaaaatca tgctgggcgt
     9661 tggccctaat agcaagcttt gagggcttaa cacttctttt agctgattgg caatctgaaa
     9721 gattttcttt gccgctactt gttcttcagg atggctggcg gttattttca cagtaaagta
     9781 ataaggagga tagcctccac gatgccgtac atgcatttcc tgctgataaa actgctcata
     9841 attttgtttt ttagccaatt ctatggcgta atgttgagga ttgaatgttt ggatcacgac
     9901 ttcgcctgct ttttccgctc gccctgctcg tcctgaaact tgggtaagca attggaatgt
     9961 atgctcacta gaacgaaaat ccggtaaatt cagggaggta tcagcattca acaccccaac
    10021 taatgtcact tctggaaagt ccaaaccttt agcaatcatt tgtgtcccta ataatatatc
    10081 tgcttctttt gcaccgaatt tttgtaaaat ctgttcgtgt gccccttttc ttcgagtggt
    10141 atctacatcc atacgcaaaa tcctagcttg cggataaagt tctcggagtt cctcttctac
    10201 tttttgagta cctgttccat agtagcggat tttattgccg ccacaattag gacagcgatt
    10261 gggaataggt tcttcatgtc cgcaataatg gcagcgcatt gaacgtgtat ccatatgcag
    10321 tgtaagagag atatcgcaat taggacatgg aagaacaaat ccacagtccc tacacatcac
    10381 aaaagacgaa tatcctcgac gattgagcag taggacagtt tgttcttttt tgtctaatcg
    10441 attttgtatt ttttcctgca aattagcaga aaaagtagag gtccgatgat tctgaaactc
    10501 ttcacgcata tctacgatct caatagctgg aagctgtgca gctgctttgg ctcggtggtt
    10561 taactgcaat aactgataaa cgcctttttg agctcgtgct cttgattcta aagagggcgt
    10621 cgcgcttcct agaactattg ggcaatgatg atatttactg cgccaaattg ctagatctct
    10681 cgcatgataa cgcggtgtct catcttgttt gtaacttgat tcatgttctt catcgataat
    10741 gatcagcccg atatttttca gcggggcaaa aatagcagat cgtgcgccta ctactacttg
    10801 cgcttcttcc cgctcaatct tgcgccattc atcatatttt tcaccttggg aaagtccgct
    10861 atgtaagacc gcaactgatt ctccgaagcg tcctttgaat cgctcgacca tttgcggagt
    10921 caatgcgatt tcaggcacta acataatagc cgttttattt tctgacaaaa catcagcgat
    10981 tgcctgaaga tatacttctg ttttcccgct gccagtgatt ccctccagca aaaagacctg
    11041 atctttttgc tgctgacctg ctgtgatgat ttgttcgaca gcattttttt gttctgcatt
    11101 cagttccaat gccgtcgttt ggtcaaatac tcggtcttta taagggtcgc gataacgttc
    11161 tgattctata aactccagcc agcccttttc ttttccttga ttcaagacag cagtactgat
    11221 tcctttattt ttgaaataag caattggttc ttcttggctc agttcagata aacacaaaag
    11281 aagttctttt tgtttcttgg cattttgccg taattttgcc cattcttctt cgatttgctt
    11341 ttcagttaac aaagaacgga ccaaacgaat cgtttttact ttatttcgtg tatggacttc
    11401 atatttgata tctactaatt gctcttggcg ccagcgaatc aattgagaaa ggctgccttc
    11461 ttcttctgct tccttccagg aaatcacttc tcgctgatca aacaatacct gaacttctgg
    11521 tgtgtctgcc aaaggataga tcaatttatc atattctgct ttcatcacac tcggaagcat
    11581 cgtttgtaga caagtgattt tgaatgcgta agtaattttt ttcatataat cagctaatga
    11641 aagtaattct tcattgacca ccggagcaag gtctagcaaa cgaataactg ccttcaaaga
    11701 tggattagct gattcttctg atttttggat agccataaca aatccttgga catgacgatt
    11761 gccattacca aaagggactt ctactcgcat cccgacttgc aaagctgttt ccatttcaga
    11821 gggaaccaaa taagtgaatg gctgatcggt ctgcatagta ggtacgtcca caatgacttc
    11881 agctgttaat gtcatggttc gtttcctcct ttttttgtct tttcttatca agcgacaaaa
    11941 agttccttgc ctattttact agagatcgat ggatttgtct tatcttctgc caaatcctaa
    12001 caaaaaatta acagcagata agaaaaaaaa gaactgtgtc aaaaagttca gacacagcct
    12061 tttctaccag acaataagtg gcgaatgttt gaaaaggaaa cagctgggtt tgcttttctt
    12121 tttagatgtt cgctctattt ttctttatta cattttttgc tcttcacgga tacgtgattc
    12181 caattcgtgt tgttcacgct ctttttgtgc ttgacgttcc tcttcttcca tcttcaaacg
    12241 ttcacgtttt aattcaggat gtggatcatt gactacattt cctgcatcga tttcttctaa
    12301 agcttgaccg acgcttttta ctgaatcaaa agaatctaga gtaggctgtg cacttgcatc
    12361 caattcatgc gcgcgtttac ttgctaaaat cactagagaa tattttgaat ttacacgatc
    12421 taatagtgag tcaattgaag gttttaacat cataagacta catctccttt aacattttaa
    12481 tatatttacc gattacgcga tctactcgaa aatgttcact ggcaataatg tttttgattc
    12541 gttcaactgc tagcggcact tcatcattta caacagcata gtcgtaaaga gccatcattt
    12601 cgatttcttc ttttgcaact ttcattcgtt tttcgattac ttcatctgca tctgttcctc
    12661 ggcctacgat ccgtgatttt aattccgcta aatctggcgg agtcaaaaag atgaatacac
    12721 catcgggcac tttttctttg acttgttttg ctccttggac ttcaatctcc aagaaaacat
    12781 cttttccttc atccaatgtc ttgttgacat atgacaaagg agttccataa taattcccga
    12841 catattctgc gtattccagc atctctccag cttcgatcat tgattcaaac tcttctttgc
    12901 ttcggaagaa ataatcaacg ccttcgacct ccccttcacg cattttacgg gtagtcattg
    12961 agatcgaata ctgaaaatca ttatcctcac tctcaaaaat tgcttttcgt actgtgcctt
    13021 ttcctacacc ggatggacca gacaacacga ttaataatcc acgctctgac atgacgacgt
    13081 cctttcaact agtctttttg tacttatcgt ttattttgca ctttttttct gcaactttca
    13141 aggttatctt taattatagc tagaaattgg taaaaaagct accctgtacc aagaggtcgt
    13201 aaaaaagctg tcctgcatca agtaataaga acagccaaac ataaacagct tctcatgttt
    13261 ttcgtttggt tggggttatt atcgcagatg caagcttttg aacgccgtgt ataagaggtc
    13321 gtgaagaagc cgattagttt cgagtatgaa gaaaaatttt gtgaaaatag tttctcacat
    13381 ttttgcataa atttggctta ataccgagaa aatggttttt gaacatcact tattcaaaat
    13441 aaaaaaagat tgtgacacga attttgtcac aacctttaaa tgtattccta gagcaaagaa
    13501 gctaagcgaa gaaatccaat tctgtttatt tattgtattt ctttgttcag catccttgct
    13561 aaaagcccat attggacaaa gtattcacta cacccatgat ctcatctttt cgagaacgtg
    13621 ctttttctgt cggatcttct acctcttctg tgtcaattcg aacaataaat ttccgattca
    13681 tcgtcacgta agcaccgtcg ttgatcaaaa taaacagttc gttctcatta tagtctgcac
    13741 aggcatcttt ccatacgtca aagccgtttt tttcagaagg gatatcccgt ttaaccacag
    13801 tttttccact taattcgtgg aacgttacgt cataatattt cattatctct acctctttcg
    13861 tcattcaata ttttgtactt gttctctgat tttttctaag atcgtcttag actggacgac
    13921 ttgattttta atttcgattg catttgattt ggaaccgatc gtgttgattt cccgattcat
    13981 ttcttggatc aggaaatcta attcccgacc tacaggacat tgtgcttcga taagttcatc
    14041 tagtttttgt acatggatcg ccagacgatc cagttcctcg tgaatatctc cgcgttccaa
    14101 caagatggct agctccgtca acaaccgttg ctcatcgact ttcgctccaa gaaattcctc
    14161 cagctttttt tgatacctta tttgaaattc cttttcatag atttctacaa aactatcgag
    14221 tttttttagt acttctttga attcttcagt attctttttc aacactaaag ctaaagcttg
    14281 accttctttt tctcgactcg cctcaataga atcaagtgcg cttttcaatg tatctagagc
    14341 taactgtgcc aattcttcca tgctctcttc cggtttttct tctaccgtta cgaagttttc
    14401 gtccagcacg aagcgttccg ccatccgact aattgacact tgattttgac taccgtatcg
    14461 ttgttgaatt ccgtctgtca attgagtgat aagtgaatca atcaattccc agttgatgac
    14521 gatctgtttt tcgtttttgc caatataagc tagattcaca aatacttcga ttcgaccgcg
    14581 gcttaactgc tgtttaataa gctgacgaaa atcgttttca acaaagttca ggattttagg
    14641 acttcgtatt tgaacatcta gaaaacgctg gttgacactt tttatttcaa tatctaactg
    14701 atacgctttt gtttcgcgga tggctttgcc aaaaccagtc atacttttca ttcgacaggg
    14761 tccttcctca ttacatattt tctattgctt tttgcagttg ttcaaactct tcgttcgtca
    14821 atgtcaggcc tttccccatt ttttcatggt ctggcgccca atctcgaaga tcgaattttg
    14881 gcgggcgacc attccagctg actaggttca gttctttacg ccaccccttg ttgttttctg
    14941 acaatatagc gatttcttct acgatttcat atgaaaattc ttgtgccatg gttgttcctc
    15001 cttgagataa tataaatata ggatcagtaa tgtgtcaact ggttgcttaa gcaattcatg
    15061 ctgcaaacga tccttgcgtt ttccttgtag cagataatca aaataaacag ttttcgtgcg
    15121 ctcattttgt attttttgct ggtattttcg ataccttcgc cattcttcag tacttaattg
    15181 accaatttgt gccaataaat catccggcat actttttata tcttgttggc agatcatttg
    15241 aatcatcatg tctacttgat agatttcatt gggcagtaaa aaatgttttt ttgctaaaag
    15301 ttctagcatc gccaatccgc atgtctctgg tacgaccctg ttcttatata aattacgcag
    15361 tttttgccaa aattttatgc ttttgaaaaa agataattct aactgaattt cttttgttaa
    15421 gtaagacaaa aatcctcgcc agtatgttct tgcaggttta acagaatgaa atgtatacca
    15481 gttcccatag taatagccta atcgtttttt catttccaga tagcctaatt gtaaatttaa
    15541 ttggttcctt tggctatcaa aaagcagaaa gctgcctagt gtccacaatg tttgacattt
    15601 ccaatggata aaaatgtctg gcatacgaat ttttttcgct gggcctggcc cttgcacatc
    15661 tacgacaaat gcctctgttg ctcctttgtt aatcgcaata tcaataggta ttttgttgcg
    15721 atacccaccg tctgcatatt tattttttcc gatctttcga taagccatag cgggataaaa
    15781 tgaagcagag gcgagtatcc aatctggtat ttcttctgtt tttgtctgtt ggatatcagt
    15841 cactacttcc gctagttctg gcagtttagt agacaccgta tataaacgta ttttgggatt
    15901 atgtttgagt aaggacaaat ccaaattgtc tttgaccatc ttttccaatg gagcagaaga
    15961 aaccccgcct tcaattagtg ctgttttcgt catttggcgt ctttctcgat agaagcgttc
    16021 acgaaggtct tcaactgcag ccatttctgg taacgcaagc acttgtcttg tcgacagctt
    16081 tttccataaa ttcacagctt ttttcatatc tccttgtaaa atcaatgcgc cgttcaacgc
    16141 acctaccgag gtacctgtga taatctcaaa gttgatatca tgttccttca gcgcctgcca
    16201 cacaccaatc tgataagcac cgtgcgctcc tcctccacct aaaacaagtg ctgtatgata
    16261 gaccagcttt ttatttaatt gtgtggttcc ttgatagccg tttttatgaa aaatacgcaa
    16321 ccattcttct gatagaggag attgaaataa aaaacaacag ttttctttaa taaaaaaacg
    16381 agcgcaagct tccatatacc gaaaaaattt tttccattca aaagtttcac cttcaatcaa
    16441 cagattatta aaaataacat ctcctctact tttagcaata gtaaaataga cggtatattt
    16501 tttcgatact gctttaaaag cacattgctt ttttgcagaa cgccaaaaag cgtctctact
    16561 ttcttgatga ctttgaaaaa atggaagggg ttgttttgct tgctggatag gcgaatacag
    16621 cgacattttc tgtgaaaagt aaactttttt tatctccatc gcgctctcct ttttcttcaa
    16681 gtataccagt acgtcataaa aaggtcatga gtctcgtatt cgactttgta gattccataa
    16741 gaaaacaatt cataaaacct cctttaaata aaaaaataca aaaatcacaa aaattaattg
    16801 aaaatgagag tgttttcaat gaaaacgtga ttttgtaatg gaattttaaa ataaaagaaa
    16861 caagcttttt taatgcaaat gttgtaaaat aaaagagatt aagaggaaat aagaggagga
    16921 acatgatgac tgagtcagtt tatgtacata ttgataccac aagtaatgct gttttaacaa
    16981 aagggctgac tgctactgac tttgcaaaca gtatcgtgca ttttcctcaa aacttgctct
    17041 tgcttgatcc atctgcttct gctggagaat atgaatccca tacagggctg aaagtaatcc
    17101 gtggtacaga aaatattcag cgtttctttt cctcttctcg taacaggcgt ggattcgatg
    17161 aattgaagtg gatcgacttt actgatttaa cgatgctgaa agaattgaca cctttagaaa
    17221 tttcagaatt attatatttt ggtcatatga aaacacatct tcattcgccg tttttttata
    17281 aactacagaa taattttgta tacttcgatc ttaatgatca gctgaatcgg atttattatc
    17341 gctatttaga tgaattttat aggatttttg ctagtaaatt aagttcgatc ttgtcagata
    17401 agatcaatgt aaaaaaatct tttttcaaaa aagaaacacc tgttgaaaaa atcagtgtgg
    17461 aattattgaa agaaatgcgt aatttgatgc aggaagggat cattttttct tttgatcaag
    17521 ttggtttgat cgatggtaaa tatcgtatcc caatctatgt gattgaagat tatatttgga
    17581 aattaaaagg cacccggtat cgagaagagg aaatcatcgc tactttaatc tactctgtca
    17641 ccgatcgaca ctggcaattg gtagaagatg ccgatctttc ttatctgcca ttgaagtaga
    17701 tctatttctt atcaaaaaat aaaaaggaaa caagaaacta ggacatgatt ctgtctcaag
    17761 tttcttgctt cttttttatt tacagctcct gtcgtttatc tggcacaaaa atcgaaggag
    17821 tcgtttgttg gatccctaat aaaacgatga tcgttacaat agctgtactc aacccgctgg
    17881 caccattcat tacgaaagaa aagagccaag gattcatccc ccatagtgca tacgctcccc
    17941 agaaaaccac tcctgcaata aaatgccaaa aatatctgga aagtgcgcca atcaatgcac
    18001 caaacacgat gctttttcta atttgtttcg tattatcgtc tacatagcct ttttggacat
    18061 ttgaagccat gagccctgct gcgcctgcaa aagtataggc aatcgggtac tcaatcaaca
    18121 cttgaacgac acttaaataa taaacttgtc ccaacggaaa atgtaggatg ccccaaatga
    18181 aacctgccag catccctgcc tttgtccctc ttcttaacgc aaaaagagtc aatggaatct
    18241 gccctaatga aatagaaaaa ctgctgccga tctggatcgg aatcaacgac aacaccatcg
    18301 ctaacgctgc aacaatcgtt ccttctaccc aaactctatt ttttcccata aaaaaacttc
    18361 ctcctttgaa taaaaaagtc acaaaggagg aagcagcttt cgaaaatagt caaacagctg
    18421 tttattcaca attcctacgc tcgcataacc gatacaggta cgaagggttt agaaatagat
    18481 tcaatctcag cccaattgga ctcccctttg tgagtttatt tattatttat tttttgtttc
    18541 aaacaatgga ctaactggtt tgttttcatg gatacgtttg attgcatctc ccattagtcc
    18601 gccaacactt acttcatcga ttttgtcgat tttacgatct tctggaagat agatcgagtc
    18661 tgtaacaact agacgctcga tagctgaatc ttctattctt tgtaaagcag ggccggataa
    18721 tactggatgt gtacatgaag catacacact agttgcacca gcttctttca aagcattggc
    18781 agccaatgtg atcgtacctg ctgtatcgat catatcatcg atcagtacac aagttttgcc
    18841 tttgacttgt ccaatgatat tcattacttc tgcaacattt gcttttggac ggcgtttgtc
    18901 tatgatcgca atcggagatt tcaaaaattc cgctaattta cgtgcccgtg tgacaccacc
    18961 atggtctggt gaaacaacga caacatcatc gcctttgatt cctttttcta agaaataatt
    19021 ggcaataagt ggcgcaccca tcaaatgatc gactggaata tcaaagaaac cttggatttg
    19081 gactgcatga aggtccaaag tcagcattct tgttgcacca gctttttgaa tcatgtttgc
    19141 tactaatttc gctgtgattg gttcccgtgc acgtgcttta cggtcttgac gcgcatagcc
    19201 ataataaggc atgactacgt tgattgtttt tgcacttgct cgtttcagtg cgtcaatcat
    19261 gatcagcaac tccatcaagt tatcattgac tgggctgctc gttgattgaa taacatagac
    19321 atgcgatcca cgaatacttt cttcaatgtt tacttgaatt tctccatcac taaattgagt
    19381 aacagatgat tttcctaact cgacgccgac tgctgctgca attttctctg ctaatgggcg
    19441 attggaattt aacgcaaaaa tttttaatct tgggtcaaag taatgttttg acatggggac
    19501 ctccactttc tttttcacaa ctctagaatt ctacctaata aatagtagtc atatttttta
    19561 aataaatcaa gtgattttgc aaaagtctta gtttagataa gggagttttt ttgcatatcc
    19621 ttctttattg acttgtcttg ctctcgcaat tgccagcgca tattctggaa tattgtctgt
    19681 aattgttgac ccggcagcaa tcgaactgtt tttagctacc tcgacaggtc cgatgatatt
    19741 cgtgctagaa ccaataaagc tgtggtcgcc gacagtcgta tgatgtttgt tctttccatc
    19801 atagttgacg aagacaacac cacatccgac attgatttct tctcccagcg tagcatcacc
    19861 aacataagtc aagtgaccga ctttagttcc tttgccgatt tgagcatttt tcacttccac
    19921 aaagtttccg atatggacgt tttcgccaat ttcagctttt ggtcgtaaat gtgcataagg
    19981 tcccacatct gcatgtttct tgacatggct gctttcaatg actgagtttt caatgacgac
    20041 gtgatcctca atcctactgt ctacgatttt tgaatgtgac ccgatgacac aatcagaacc
    20101 aatgactgta tttccttgga tttgtacacc tgcttcgatc aatgtgtctg gaccgatttc
    20161 tacgcctgca tcgatgtatg tcgttgctga atcgacaaag cttacgccat tgaccatatg
    20221 catctggtta attcgctttc tcatgatctc atttgcttta gctaacgcga tccgatcatt
    20281 gactcccatt gattcttcaa aatcatccgt ttgataagca gcaactgtat ggccttcttc
    20341 ttttaagatt tcgatgatat ctgttagata gtattcacct tgagcattgt ttgttccaac
    20401 tttgcttaac gcatcgaaca aagcttcgtt gtcaaaacag taagttcctg tattgatttc
    20461 ttgtacaaga gcttcttctg gtgttgcatc tttttgttcc acgatttttt ctacgatccc
    20521 aatatggtca cggatgattc gtccatatcc tgttgggttt tctgcttgtg ccgtcaagat
    20581 cgttgcactt gcattttttc cttggtgata ttcaaaaaga ttgttcagcg tttcagtagt
    20641 caataatggt gtatcgccac tgataactaa cgttgttcct ttttttcctt ccaagaaaga
    20701 agcagcttgc aaaacagcat gtcctgttcc caattgttct gcttgtaacg catatttact
    20761 gcgttctcct agttgtgctt taacttgttc tgctccgtgt ccaacgatcg taatcacttc
    20821 atctggtttc gtttcactga cccgattaat gatatgttct accataggtt taccagaaac
    20881 gggatgtaaa actttatata attttgactt catgcgggta cctttccctg ccgccaaaat
    20941 aattgcataa cgtgcgtcca actgcgtcac tcctaaatat agtttgctga tttcagttta
    21001 tcccagtata cccatttttt caacaaaaca agtgattttc ttttatccca gcgttttctg
    21061 gataaaaaaa tagatagacc ataacgggcc tatctatttg gtaaccggtg ctctcctgcc
    21121 aactcctcgg tcacaagtca cactactcat aaaacatgaa aagctgtttt ttgaaatcgt
    21181 ttcttgtgac ttagagttaa acggtaggct tacaacctct tcattaaacg tttttcaatc
    21241 agctgacctt cggtattagc tcaataaacg gcaaaatatg aggagcaatt tttccgttta
    21301 ttgagctaat actcaggaca ggacgctgat ttcacaacct ctagataaac ggtgttcaaa
    21361 agccagtttc tcggtattaa gccaaattta tgaaaaaatg tggaaagcca ttttcacaaa
    21421 atttttctta ctactcgaaa ctaagcggct ttttcacaac ctcttattct gaaaaataat
    21481 ttcctggcac aaccgtaata tttttcgttt gagtatctac atctttgaca taaagcagtg
    21541 acgtataatc atcgatggcg cgatgaccgt tgaattttga ttcggcaaat acagtgatgc
    21601 caacaagttc tgattcgaat tcttcaatca agctcttcat tccgttgact gttccgccgc
    21661 ctttcatgaa atcatccact acaaggacac gtgaaccacg tttcaaactt ctcttagaca
    21721 gttccatctt ttcaattctt tcagaagatc cagacacata gttgacactt actgttgatc
    21781 cttctgttat ttttgagtcc cggcgtacaa taacgaatgg cgcattcaga taataggaca
    21841 cagcttgggc aataggtacg ccttttgtgg ctacagtcat tacagcatcg attttttcac
    21901 caaggtattt agaagcaatg attcttccaa cttgacgcag cagttcaggt tctcctaaca
    21961 aatcagagag ataaacatat cctcctggaa gcagacggtc ttgttctgat agacgttctg
    22021 ctagagattc aatatatttt ttcgcctctt catatgaaat ttcagggatg aacaatacac
    22081 cacctgcagc acccgggatt gtttccaagg ttccataccc tcgttcttta aatgttttct
    22141 tgatgattcc caaatcttca ctgatagaag atttagcaga ttcataacgt tcggcaaagc
    22201 ttgttaagga aatcaattca tgcgggtgat tcaataaata ctgtgtcatg tcaactagac
    22261 gttcactgcg acgtactttc acctattcca actcctttta agaaatttta tcgttttaca
    22321 ttatataagt ttttcttcaa aaattcgagc attttatgaa aaaataagaa aatagttcgg
    22381 tatttattaa aataaaaaga agcctataaa aaatttttcg tttttataag gcttcttttc
    22441 tttataattt agttattttt tcttctgaag gcaaccaata ttcgtttgat gatatttaca
    22501 atcaggaaca aagcaataaa gactaatgtg attgttgctc ctggaggtgt atctagataa
    22561 aacgagctag ttagtcctga cagcatccct agaaatccta cacaaacact gatagcaatc
    22621 acgacattga agcttttgcc taatcgcata ccgattgctg cgggaagaac catgattgcg
    22681 gatatcaaaa gtgcaccggc aatcgggatc atcacagcaa tagccacacc agtaatcaca
    22741 ttgaacatca tcgacatcaa atgaacgggt agcccatcca cgtgggcggt atcttcatca
    22801 aaagtcagaa catacatcgg ccgtttgaat aaaaagaaca atataccgat ggcaacgaat
    22861 aagattccta aaaagatgac ttgctcccat gtaatggtca cgatcgaacc aaacagatag
    22921 gattggatac ttgttgctga attccctccg ctcaaattca tcaatactaa cgctagcgcc
    22981 aaaccaccag ccattaaaat ggcaatggag atttctgaat aggttcggta gatcgttcgc
    23041 agatactcca atatcacagc tgctaataca acgactagca tggtcgtaag tgtaggatta
    23101 aggttcaaaa agaatcctaa cgctacccct gctaaagaga catgagaaag cgtatcagcc
    23161 atcagcgatt gtcttctgat cactaaaaac acacctagca tcggggcgat tccggcaatg
    23221 aagatagcgg caagaaacgc tcttctcatg aattcatatg aaagcaacgc cattgagaat
    23281 cctcctttct caccagtcgg atctgtctgt ccgcatactg tttgatatct tcatgatcat
    23341 gggtgatcat tagaactgat ttcccatgga catgcgcact atgtcggaga agacgataaa
    23401 attcatttct ggattcttca tccatacctg ttgttggttc atcaagaaca aataagtcag
    23461 ggtcagtagc aaaaatacgc gctaaactga ttcgctgctt ttgtccgcca gacaattcac
    23521 ctattcgccg atttcgcata tcccacatcc ctacagcttc taaagcacgc tgtacatgtt
    23581 gatgatcttt ttcagagaga cgcttgaacc agcggcctct tggatatctt cctgaacgga
    23641 ctaattcgat caccgtacta ggaaaacctg cattgaaaga tgcaacttgt tgaggaatat
    23701 aaccgatact taattttttt ccttcttgat ttttctcagc aatctttatc attccttttg
    23761 aaggtttcag taatcctaac gatgctttca ccaaggttga cttggctgct ccattttctc
    23821 cagttaaaat gacaaattca cccgcatcta catgataaga gacatcttct agtacaggtt
    23881 catcatcata gtaaaaggtc aaatcagata tttcgatata acgcacctta caacctccat
    23941 tctaaaactt aattgatact ttcttttaaa gctttcagat ttttttccat gacagaaata
    24001 taattttcgc ctgctttgat ttgttcatta gtcaagcttt ctaaaggatt caacacttct
    24061 agcttcactc ctgtttcatt cgacaatgtc tcagctacct tcgaagaggc attttcttca
    24121 aaataaatca ctttgatgtt gtttttcttc acaaattctt ttagctcagc taagcgactt
    24181 ggtgtgggtt cctgatctgg tgaaacgccc gaaatcgcta cttgttttaa gtcgtattga
    24241 ttcgctaaat aagcaaaagc agcatgctga gtgacaaatg tgcggtttgt tgcatctttt
    24301 agctcatcgg agtatttttg atccagtgct tcaagctttt tgatataagc atcggcattc
    24361 ttcgtaaaag tttccttttt atctggatat ttttcgccta attcttcagc aatcgttctg
    24421 acttctttta tagctaatac cggatcgagc caaacatgtg gatcgtattc atgtgaatga
    24481 ccatgccctt cttctgattc ttcatgttcg tctgcttctt gcgattctaa acggtcgatt
    24541 cctttagctg cttcgatcat caaggtctgt ttgctatcca ctgaatcctt taacgaatcc
    24601 acaaaaaatt ccatgtctga actattatac acgaaaacat ctgcatcgga aatttctgct
    24661 ctttcttttg cacttggttc gaaatcatgc ggctccgttc cagcagggat caaaagtttc
    24721 acgtttcctt catcccctac gatctctttt gtaaaatcat acatcggata aaaagtcgta
    24781 acgatcgtca aatcctcttt tttgtctgct tccttgttcg tatttccaca tgctgcaaac
    24841 agtaaagcac ctatcattac cgtagctgtc aaaagatatt ttttcattta tatgctcctt
    24901 tcaatgaaca aatcggaatt ttacgattta ttaacgtgaa ctaatgtatc aaaataaaaa
    24961 aacacagtca atagaaatcc atcaatttct gatttaagca tgtatattct tgattaaatc
    25021 gtaaaatgga tgagttgctg ctcatatcta gccatttcac tacaataaaa aactgaagaa
    25081 taccatttta ggattcctca gtttttcatc acctatttta cttcttatat tttattgcag
    25141 ggttcgtact aaataaactt cctcacaaaa accttttaac ccattatata ctctctgcgc
    25201 tcttgaataa tgttgacaca aggcaaaaac ggttggtccg cttccactca tcaatgcagc
    25261 atctgctccg tacttgatca tacgctcttt gatttgatgg atgactggat gccgctttcc
    25321 cgttatatct tctaaactgt ttcccatttt tttgatcatc tgctgataat cttgctctcg
    25381 gatggcttca gcaagtccgt ggatgtcttg atgttggatg gcttgcgtat ctacttctct
    25441 aaacactgta cgtgtagaga cactcattct tggtttgacc aacacaaccc agcactgtgg
    25501 catagaaggt aatacttgga tttcttcccc tttgccaccg acaaatgccg tacttccgta
    25561 gacacagtaa ggaacatctg taccgacttc caaaccgatt tctgccagtt cttctaacga
    25621 acagcccaga ttccataatt ggttgatccc tcttagagca gctgcacagt ctgtacttcc
    25681 tccgcctaat ccagctgcta ctggaatatt cttcttaaca aagatcttga caccggtttt
    25741 gatattgtat ttatttttta gcaataaagc agcttgataa acattgttcc gttcatctac
    25801 cggtaaaaat gctttatttg tttctacaat gattttgtct tctgttattt cttcaaaaaa
    25861 tagatgatct gccaagtcta cgctcgacat caccatttcc aactcatgat acccatcttg
    25921 ccgtctatat aatacatcca gtcctaaatt tatcttggct ggcgcttttt caatcaattc
    25981 catcctctca cccattcgaa aagtctaagt cgcaagtatt ctatcatagg gaaaaataaa
    26041 agtatagctt atgtctgcct agtggttgat tttgtcatcg tttcaacagc tttcacagaa
    26101 ttttctcttc ataatccaaa caagttctag tgattcttat aattctgtga gcacttcttc
    26161 tgccaaactc ggaacatttc ctttttcttt tcctatcaga ctgagtagct gcatggtagc
    26221 aaatccgaaa ttacttcctt tcctataagc aaaataacgt ccttcccaat gatcgacata
    26281 ttttaatttc ttttcataag cagactggaa ggaatagaaa aaattaccat aattatatac
    26341 gatatgcatg actttttctt gaaagaaaga aaaatctgtt tctccaacat tagctaatgg
    26401 agctaagcct agattggctt ccgagtagcc cattttctcg tatacaataa acagattggc
    26461 taacagatag tcagctaatt cctcagattc tttaggtaag acccgcaaca aatcatatcc
    26521 cgcctgcttc ttcgtcatcg gctgagcggt gatgaagcca acgacttttt gatctttacg
    26581 gacaataccg acaccgctag ataatacata tgatttatca aatcgtccca cagagaagta
    26641 tttttctcgt gcaccttgaa gccatttttg tgaaacttca tttacagctt gatgtaattc
    26701 ttccggcagt tgatcgtacc aagtaaaatg gtatccttct ttcatcaaac gttgatattc
    26761 aagttgctga ctcagccgag acaagtcttc tgttgctaga tcaacaatcc ctgcttcacc
    26821 tactttcgtg aagtgaaaac ccaattcatg aagcatcact acgtagcttt ctgatatctt
    26881 ataaaacgca agctgatagc ctaaacaatc tgcttgtttc ataaactgat tcgttgctgc
    26941 ccgcatcatt ttaggattgc caactggatc tcctaaaaca aagcatttgt ttcctttgat
    27001 ctgataacca aaaacgactt gatcttggtc gtcttcttgg taataaaaat aactgtaacc
    27061 tgacaagcga agctgatggc ttgtagctgt gcctccgtat gtttccaata aacggtcaaa
    27121 acggtctttt tcgtactcac tgccaagctt cgtagtatct tcagctaaat attgataaag
    27181 cgccactaaa gctagcaatg agatgcccaa accaataagc cctgagaacc acacatcatc
    27241 tgaaggaaac aaaataaaat gatttcctgt tattcgaccg actaaaaagc gtccgctgtg
    27301 atagcctgca attgcgtaaa cgataaacaa aaagccgaac aagctagcat caaagattat
    27361 agctccccaa gaataaacga attttgttcg ataaaattct tttctggata accacacagc
    27421 taacagaatc aagacatata ccaatatcaa ctgccaagat aacgtacgtg tgattgtatt
    27481 taaaatacca aaagatagta aagcaatcgt aggcagaaat gcctttttga cttttactga
    27541 aagtgctcta gcaaggccga gtaataaaaa gccaaccaat agattcactg tctgatctaa
    27601 aaaattgaat gagaacggca ataatacttg aaatagccga ctgattgcag acagattagt
    27661 gattgttgat agaagtacca tcatgatccc cgcaaaatag acagcactta cgagcacgat
    27721 atgcgcaacc ttttggaaaa acagtcgcgg aatgttatct aaaaatcgat tgattttgac
    27781 tcctgtatgt gtgatgaaca atagaagtcc cgtcataaaa ggtagaacat aataaaacaa
    27841 ccgataatat aatagccaaa ctactgctac atcttgccga acagacaatt gactcattcc
    27901 taagatcatc aaaacgtcaa aagtccccat tccacctggg accatcgtca gcattcctat
    27961 caatgtagca atcatgaaca taggatagat cgaaagcatc gaaatatcta ttctcatcaa
    28021 cttgccgatg atcaaaaata ccgcaagggc tcccgtccat tgtccaaagg atgccagtag
    28081 cagaccaagc aagccttttg gataaaactc gaagaacaat gtctttctct ttagataagc
    28141 aaaaaagaaa atgactggtg ctaaaaaact accgccgaaa agccagatcc aatagcttcg
    28201 aaaaatactt tctgactgaa taaaaaagac atcaataaat gtaagaaacg accatacaga
    28261 caaaccagtc agcataaata gtgcgacttt tgatacagtg gctaaaacca tttttctatt
    28321 tgtctgcttt ccataaaaac tagcccgcag acttgctcct accacaccac caaaaccggc
    28381 gagattgttg atcgtgtttg ttacccacgc agcaagaaga aactcttttc tttccatttt
    28441 tggttttcct tgtttttcta agactctgat cgtcacccaa tcatacaaca acatcggagt
    28501 gacacccagc agtccagcca gaatcatgag caggatcgtc atgcggcttt gttgtcccat
    28561 cctttgccaa atatcttgcc aactcatgcc attagcaata tttgcgactt gatttgccac
    28621 aaagactaac acggagccga aaaaaatcag ttttaaaatc agactatgtt cctttatcca
    28681 ttgcagcatc aagtatcgtt tatttttcac attttcccat tcctaacttg tatacttttg
    28741 tgtcataccc caatgttatc ttatttattt taatttttcc actttagacc tgttctttgc
    28801 ttgcgatgat agaaaaagaa agatatccta atgctaaagg agaggataac atgaaagtag
    28861 gatttatcgg cacagggaat atggcacaag ctattattga tggtttatta aaaaaaggaa
    28921 cagtctcgaa agaagaggtg ttcgtttcca gtggacattt tgaaaatgca caaaaatttg
    28981 ctgctgaaac tggcgtccat gcatgcaaaa gtaatcagga agttgcatca caatcagata
    29041 tcgttctttt agccgtgaaa ccaaaaatca ttgaaaaagt attggaagaa ctaagagaat
    29101 atagccagaa agtcttattc atttctattg cttcaggaaa atcattaata gatttagaaa
    29161 cagcgcttga agatcctcag gcctccatcg tacgtgtgat gccgaatgtc aatgtcagcg
    29221 taggagcagg cgtttctgcg atttgcaaaa atgaagctgc cacggaggaa cagcttcagc
    29281 aagctacgat gatttttgaa gcaatcggca gcgtctatcc tttagcagaa tcagatttca
    29341 gtacattcat tggccttgct ggcagctcac ccgcattcgt ttacatgctg atcgatgcga
    29401 tgggacggac tggtgtactg aatggtttgt ccaaacagac agccactgag attgctgcaa
    29461 aagcaattat gggttcttgt caaaaattcc tttcaagtga taaaaatcca tgggaacttg
    29521 tagaccaagt ttcttctcct ggcggtacaa ctgttgcagg tgtagttgcc ttagaagaag
    29581 caggattcat cccagcaatc atcaaaggaa tcaatacgac gatcgagaaa gatcttgaga
    29641 tgttgaataa aaaataatga tagcaatgtt ttttcgtgcc ttacaaaaaa agaagagctg
    29701 tggcttttgc cgcggctctt cttttgtatt ttttattatt tagcataatc agttgcacga
    29761 gtttcccgaa taacgattac tttgatgtgt ccaggataat ccagttcttc ttcgattcgt
    29821 ttacgaatat cacgtactaa acgaacagcg tctaaatccg agatttcttc tggcttgacc
    29881 attacccgaa cttcgcgtcc agcttgaact gcgtaacttg attctacacc ggcgaagctg
    29941 ttcgaaatat tttccagact tcttaaacga cggatgtagt tttctaatga ttcacttcgc
    30001 gcaccaggtc ttgctgctga gagcgcatct gctgcagcta caaggacaga gataacagaa
    30061 gtagcttcca catctccatg atgagaagcg atcgcgttga ttactactgg gttttctttg
    30121 tacttggcag ctaattccgc accgatctca acgtgagatc cttcaatctc atggtcaagt
    30181 gctttcccaa tatcatggag caacccggca cgttttgcta attggatatc ttcgccaagc
    30241 tctgctgcta agataccagc taattttgca acctcgaccg aatgcttcaa gacgttttgt
    30301 ccataactag ttctgaatcg cagacgtccg atgatcttga ttagatctgg atgaagtgta
    30361 tgagcaccga cttcaaacgc tgcctcttcc ccgtattcgc gaatacgttc atccatttct
    30421 ttacgagatt tctcgaccat ttcttcgata cgcgctggat ggattcgtcc atcttgtatc
    30481 agtttttcca aggtcatccg tgcgatttct cttcggattg gatcgaatcc ggaaagaaca
    30541 actgcttccg gtgtatcatc gatgattaaa tcgataccag tcagcgtctc aagcgttcga
    30601 atattacgac cttctcgtcc aatgatacga cctttcattt catcattcgg aagggtaacg
    30661 actgaaacag tcgtttctga aacttgatct gctgcgcagc gttgaatcgc taatgataat
    30721 aagtttttcg ctttgcgatc tgcttcttct ttggcacgct gttcagattc ttttaccatt
    30781 agagttaact catgattcag ctcttcttca gtcgatttca tgatgacatc tcgtgcgtcc
    30841 tcttttgata aaccagcaat tttttctagt tctttttgtt gatcttcaat cattttttca
    30901 acgtcttttt ctcgctcttc aattaattgc tgttttgaac taagtgcttc ttctttactt
    30961 tcaagtgagt tctcgcgttt ttctaacgag tcatctttac gattcagcgt ttgttcgcgt
    31021 tgaatcaaac gattctcttg agattttaat tctaacttac tttctctcag ctcactttca
    31081 acttcagaac ggtatttctg attttcttcc ttagcttcta acaatgcttc tttttttaga
    31141 gtttctgctt ctttttttgc actatcgatg attccagcag ctgatgattg tgcaccagct
    31201 atttctttct cgtgacgtga ctttgcgacc ataaacccta aaccaagacc gacaattaaa
    31261 ccgatgatag cgaggagaat gttgaatacc ataaaatcca cctccatact atctttttca
    31321 tttttatcat ctatacagct tctatgataa attattctaa actcgtcaat atgcatcttc
    31381 gcatatatgt gtacaaaaaa catgcacaac tttattctaa tgtttgtacc ccgtagtgtc
    31441 aactttaaat aaacaaagaa acggctttct acttattcat taaacgatgg tttttaaaat
    31501 aaatgacact agtttcatat aggtaaacct ttaaattgag aaacttttct attaaattat
    31561 tctttgtaga tgaacagata tttttatttt atgtacccac tcgtataatt aaataactca
    31621 cgatttcttt attcaataaa aaaacggcag gtcaaggaga gtacctactc ttcagaccag
    31681 ccgtttttgt ttatcataat ttttattctt ctactaaagg caattcttct tgtccttttt
    31741 catcttctgg gacagcgact tcttcaccaa taccataagc tgctctaact agagcagaaa
    31801 cttctgccat catttcagga tgattagcca tatagatttt agcgttttcc cgtccttgac
    31861 caatccgatc ttctttataa gaataccaag caccgctttt atcaacaatg tctttttcta
    31921 ctgccatgtc tagaagttct ccttcttgtg agatccctaa accatacatg acatctactt
    31981 cagcgatttt gaaaggaggt gctactttgt tttttacgac tttgatcttt gtacggttac
    32041 caacaatatc tgtaccttgt ttcaattgtt ctgcacgacg tacttccaaa cggatcgttg
    32101 cgtagaattt caatgcgcgt cctccaggag tgatttctgg attcccaaac atcacgccta
    32161 ctttttcacg gatttggttg atgaaaatag cgattgtttt tgttttgttg atcgaaccag
    32221 agagtttacg caatgcttga gacatcaaac gtgcttgtaa cccgacatgt gagtcaccca
    32281 tttcgccgtc gatttctgct cgtggaacta aagcagcaac tgaatcgact actacgatat
    32341 ctacagcccc acttgatact aaagcatcag cgatctctag accttgttct cctgtgtcag
    32401 gctgtgaaag aagtagttca tcgatattca cacctaattt ttgtgcatat tgcggatcta
    32461 acgcatgctc agcatcaatg aaagcggccg ttccgccatt tttttgtact tctgcaatag
    32521 cgtgtagtgc aactgttgtt ttacctgaac tttcaggtcc atatacttcg atgatacgtc
    32581 cacgggggta accgccgaca cctaaagcga catctaacgc aagagagcca cttggaattg
    32641 tagagatttg ttggtcgatt ttttcgccca atttcataat agagccttta ccatagctct
    32701 tttcaatttt tttcagtgca gcatctaagg ctgctttacg atcatctgcc aatacgataa
    32761 tcctccttat atttacaaat cagagcaaca tttcattatc cttatcatat cttttttagt
    32821 gaaaaaaagc aagaaaaaac cgaacaaata ttcgcattta tcattttctt aaaagctgtt
    32881 ttctcaaaag atcaaatcct tgcataaccg cattatatct gatagcagca cgatctctgg
    32941 caaagtgata tttttccgaa accacgccgt ctttagatga aatagcgatc catactgttc
    33001 ctgcagattg accttctaac gtgtccggtc cagccactcc ggtgaaagct acactgtaat
    33061 ctgttcttgc tatctcacgt gcttgaatgg ccatctgctc cacacattct ttgctgactg
    33121 ttccatattc tgctaatagc tccggatcaa tctccagaaa accagctttt gtttggagcg
    33181 agtaggtaac gaatccgcct ggaaatactt cagatacacc agcaatgtcg cctaatgcgg
    33241 cttggaaggc gccggctgtc aaactttccg ctgctgttac agtctgcttg ttttctttca
    33301 gtagttccac tacgactttt gctagcgaat tatcatcgcc gtatccataa aaatattccc
    33361 ctactcgttc ttggattttt tcttctaatg ctaaaagagc ttgattccct gcctgaacat
    33421 catttgtttt cactgtcagt cgtaatgtca cttcatttgg tttggcatat ggtgcgatcg
    33481 ttggattgat ctgtgtctca atcagatctt ttagctcagt gaccagtcgc gattcaccaa
    33541 tcccataaaa gcgcaaaact ttagaaatta gtttttcttc agaaggaaaa cgttcctcta
    33601 gtaaaggacg tacttgttcc acaaacattg gtttcaactc attaggcgga ccgggaagaa
    33661 ggatatacgc atgtgtatct gtttgataaa aagtccctaa agcaagccct gtacgatttg
    33721 gtagaggaac cccgccttcg ataatctggg actgttgcag attgtttttg gtcatttttc
    33781 gatgggttgt ttcaaaatat gccaataatt ttttgtatcc ttccgtattt tgtatgagag
    33841 acttaccgag gtgagccgca gttgcttctt ttgtcaggtc atcttctgtc ggtcctaatc
    33901 cgccacataa gagtatgagt tcacttcttg tttcagctag agcaatcaac tcttctaaac
    33961 gctgctgatt atctcctaca actgtttgat aataaacttc gatacctaga tcagctagct
    34021 gttctgacaa gaaagtggca ttcgtattca cgacttgtcc tagtaaaagt tctgttccga
    34081 cagctattat ttctgctttc attacttccc gctcccttat atagtatacg ctgtattttt
    34141 atattttttt atttaaaaag agggcaagga gctgtgacaa aaatcttgtc acagctcctt
    34201 gttccaaata aacagtgttc tcctgccaaa acctcggtca taagtcacaa tatacataaa
    34261 acatgaaaag ctgttttttg aaattatccc ttgtgactca gagttagacg gcaggctcac
    34321 aacctctcca ctaaacgata cttacattga tcctttgaac acaccgctgt ttttgatgaa
    34381 gtaatccaca cctgaataga tagtaaagat cagacaagca taaagcatga tttgatctac
    34441 tggaataccg atcaggctga atgggatatt attgataaac agcaagataa tagcaaccat
    34501 ttgtgtagcc gtcttgactt ttcccggcca agctgcagcc atgacttctc catgttcgac
    34561 aagtagtaaa cgcaagcctg tgacggctaa ttcacgacag acaatgatcg ctactaccca
    34621 accaggggcc tttccttggt caaccaacat gataaaagca gtcataacta gcattttatc
    34681 tgccaacggg tccgcaaact tgccaaaatt agtgacaagg cctcgcgcac gggcaatttt
    34741 tccatctaac cagtctgtga tactagctaa agcaaaaata actgcaccta ctagctgtgt
    34801 gacagccagt gtggtgtctc ctacgttaaa ttgtccccaa tctaaaggta aacctacaac
    34861 caaaatgaaa atcgggatca tacaaattct aagtacagtt aatttatttg gtaaattcac
    34921 actatcgact cctttcttcc ctattctacg tgactatcag cgaagtttcc accgatatta
    34981 tttacttttc caaatgaatt tattcaaagg agtattttta ttgaaattga tctttttgaa
    35041 ttaacgaaga aggaccgcga cacagatttg tcacgatcct tcttttttat acctacaagt
    35101 aaacagatgc gtaaactaat ttatacgatc attctgcgta ttgaagagta aggttcacat
    35161 tttttggact ctgattgttt tcccctggat taactggtac ggattctcca ttcgctttga
    35221 tcgttgcatt gcttgctgca ccaatcgtga tggtcgcatt tgttgctgtt tccggtaaca
    35281 cggtactctg tgtttcattt gctgccagtg tcccttgata aacataggca ttgtctacta
    35341 gcacacctaa ccatacacga ttgacagcag taaattcaag ggttacaggc ttggtagcgt
    35401 ctgtgatatt tactgcgatg gcactgccag aatcttctcc tgtagctatt gccattttct
    35461 tttcttcact tgattttgtt gttgattcta ctgtagaaga ctctttggtc tgcgtgcttt
    35521 gctctgagga agaggactcc tcgactgatc cttctacttt aagcgaagaa gctgtgtctc
    35581 cgattattgg atcagcttga cgatcttgcc aagtcatata gcccacgaca acgacaatcg
    35641 ccaaagcaac caatcctaat aaaatgacag gaagacttgt ccaaaagcga cttggatgcg
    35701 attcttcctg atgcattgct tttctagaag tacttactgt ttcaggctgc ggacgttcag
    35761 ggaccgatcc tgtcaatttg cttttcccat caaaaacatc gactaaatgg tctccatctt
    35821 ctcctacagc ttcagcgtat tgccgaataa atgcccgcac gtaaaattta ccaggcaatt
    35881 ggtcaaaatc atcattgtcg attgcttcca aataacgttt ttgaattttt gtaatctgct
    35941 gtaattcatc taaagatatt tttttattca ggcgtgcttg ccgtaatttt tctccgatgg
    36001 tttcaaaagc cactcgttca tcttctccag tctgtttgat tctccgaagc atctgctgtt
    36061 gcaacgaaag acgttatgtt tgggtgctgt gacaaaagtc atgtcacagc tccttttttc
    36121 cgaataaaca ttgttctcct gccaactcct cggtcacaag tcacaatatc caataaatat
    36181 aagaagctgt tttttgaact tgttccttgt gactcggagt taaacggcag gctcacgacc
    36241 tctttcttaa acggtgttca atcagctgtt cctcggtatt agctcaaaaa ccggcaaaat
    36301 ataaggagat atttttccgt atgtttctct aatactcagg tcaaaacgct gatttcacaa
    36361 cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagtcca accaagttaa
    36421 aaacattaag aagctgtttt tacttggctg ttcttattac ttgatgcagg gcagcttttt
    36481 cacaacctct tggtacacgg tgttccagaa gtagcttctt cggcaataag ccgaaatatc
    36541 acaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc
    36601 tgtcacaacc tctttgatta cttcgtttga ttttatttta atataagttg attaaaagag
    36661 agcatgattt gttttatttt ctcactaatt tttccaacac aactaagaat accataaaag
    36721 ctgattttgg aggaggttcc tactacaagt ctaaaacgaa atttaagact tttcctcata
    36781 aaatactggt ttattttggt ctcatgtaaa actcgctgaa ggcctcttca cttataaatg
    36841 cttccccagc tgccagtacg tctttcattt ggatcgaatc gatgatttca ggaagatcaa
    36901 ataaagttcg atcgccaaat aaactttgcg taaattgatt ggcaatatat tcgatcgaat
    36961 ttaatgattg gaaatattgt ccaagcattt tcttcttcag caaatcgaga tttgtttctg
    37021 acacttccgg atcatcagcg aatccaagaa tgatttcttt gactttttct gctgctttct
    37081 ctggttcgtc cgtatctccg ctgaagtccg caaagtgaaa ttcacgatcc aagctgaatt
    37141 caaaaccaaa gctgtcatcg ataattcctt gattgtacat cgctagatag tttcgagaag
    37201 tattgcctaa aatcatttgg aacaacagat tgattgctgt tttatagcgt aacagttctg
    37261 ttccttcttg cggcaatgtg tctaatcctt taataccaag tacaaattta tctcgtgtaa
    37321 tggctgcttc caatgcactt tgtttgatga tttcttttgt gttttcaggg aaataacgta
    37381 caatttcttg ctttggaggg aaattttttg cttcttggtt ttcccgaata agtttcatca
    37441 atttctcagg ctccatctta ccaactacaa ataaaaccat attgcttggc tgataaaacg
    37501 tccgatagca cgtatacaag tcttgagctg tgattttgtc gatactttca accgtgcccg
    37561 caatatcgat atgcaatggg tgtgtaggat acaagttatt tagtatgcca aaaaacatcc
    37621 gccagttagg gtcgtcttca tacatttgga tttcctgacc gatgattcct ttttccttgt
    37681 ttacagtttc ttctgtgaag taaggtgctt gtacaaaatc gatcagagtc gtcagattct
    37741 tttctacctg atccgtcgta gaaaacagat agctggtttt cgtaaaactt gtgaaagcat
    37801 tggcggaagc tccttgtttc ccaaataatt gaaagacatc accatcttct ttttcaaaca
    37861 acttatgttc taaaaaatgg gcaattccat ctggaacttt caccttttct ttttcaccat
    37921 agggaataaa ttcgttatcg atcgaaccat aattcgtact gaacaaacca tatgtcttgt
    37981 gatagtcatt tttaggcaac aaatacacgg tcaaaccgtt tggcagtact tcatgataaa
    38041 gtgtttcatt gatttgctca tattctgttt tattcatttg catttcctcc tgctaaaaag
    38101 aagattgcct gcaattcgat ttgctcagct acttgttgta tctctttgat cgttacgctc
    38161 tccatacgtt tcaaccattc ttcatcagat aattttgttt ccggcagcca gctgtccaaa
    38221 aaagaagctt cgattgccgc ttgcggagag tctagagaaa gcagatattg gtttctcagc
    38281 attgcttttg tctgagacag ttccaactct gttatttcac catttcgcaa actttctaat
    38341 tgctcgtgaa tcagatgaag tacttgattt cggttctttt catcgatacc ggtttggaca
    38401 ctcatgaatc ctcggaacgt atccacgcta ctagaggcat aataagctag actttctttc
    38461 tctctgacat tcatgaaaag tttagagtga ggaaaaccgc caaataaacc gttaaagacc
    38521 attagcgcaa aacgttctgg ttcatcgtaa tacacatttg tctgataagc taggttcagc
    38581 tttgcttgaa cgattggctc acgtacttgt cgttccttca cgatattcac ttgaggttgg
    38641 gtataaaaca gatctggata aactgtttcc cgcgactcaa atggtaattg cccaactgct
    38701 tcagctgctt tgtccggatc aacatcgccg acaacaaaaa tgtcgatttg atcatttgcc
    38761 atcatttctt gatagtagga cgctagcgaa ctagcagtga tcttgtccaa atcttcgact
    38821 acgcccaacc ctgggatctt ttgttccggg ctgttttcaa aataaagttc ttgcaaggca
    38881 taggaagcaa aagtttgttt atcttccttc aaactttcca aatatagacg cagattgttc
    38941 ttttccaagt caaacgtcaa ctgatcaaac tgatgatttt taatatttgg ataaaataaa
    39001 acctctttta aaaaatctac cgcttgagaa aaaaggtttg gatcattgac atattcacca
    39061 ttcacaaaac tgatccctgc gtttagccaa tgtaaatttc ctttttttct aactgataat
    39121 ccaaagcttg cgccataaag ctctgcaagt tgcgcactta actttgtctg atccgaataa
    39181 ttcaaactat ttgtttccaa catgctgctg agaagtgaac gtttcgtgat gacttcttgt
    39241 tgcagacgcg cagtaaaacg gataaaaatc cggatcgttt tgtattgttt tgttggtaag
    39301 acatgaagat tcacgccttc tgccaattga tacaccatat ttatatgctc cttttcacag
    39361 ttgattgccc gcttactctt ttaatgggtc aacctgcctt ttttacttat tcctctgctt
    39421 gaaatttata agttcaggca attcgccgtt ttttctaaat gatgttacac tttatagtat
    39481 agcatagatg ttttttatca aaagaaacag acggctaaaa gcgtatattg tttttttatt
    39541 ttttattttt cgatatacta aagacaaata aaagaaggga gggtcattca tgatcaaaga
    39601 attcaaagag ttcatcatgc gtggagatgt cttggatctt gctgtcggcg ttgttattgg
    39661 tagtgcgttt acaagcatcg taaaccaagt ggtgcaaggc ttgatcacac cactaatagg
    39721 ttggtttgtc gcaatgatca caggtacttc tgatttagaa ggagcacttt caattcttga
    39781 ctggtcgccg acaaaaggcg tcacatttgc gtttggtaac gttattagtg ctatcattac
    39841 atttctgatc actggttttg tcttattctt agttgtcaaa gcggcaaacc gtgcgaagaa
    39901 tttacgctcc aaagaagagc aagtggaagt ggaagaaact cctactgcag aagattatct
    39961 tagtgacata cgcgatcttc ttgccaaaca agtggcagaa gacaatacac atccagtaga
    40021 gcaaacaaat ctttccgata cttctaaata ataaagaatc taaaaaaaag aaagccgatt
    40081 ttcagtgtcg gctttctttt tttagattct taacttgcta cttgttcctt tttcatgtcg
    40141 atatgtggaa tatcatcttc caaatacaca tctgagattg ccttgaatcc aaacgactca
    40201 taaaaagctt gtaggtattc ttgtgcgccg atcacgattg gtttttctgg aaaatttttc
    40261 tggatcatgt taatcgtttc ttgaacaatc tttcttccta aaccatcttt acggttgtcc
    40321 tgatgaacaa ggacacgtcc aaaatggata ctctccgctt cttcatatac tcgcgtgtag
    40381 gcaaggatct tcccatcagt atcttgaaaa aacgtatgcc aagcttctcc atcaatgtca
    40441 tcaacttctt gataaggaca ctgttgttcc acaacaaaaa ctgcgattcg ttctttgacg
    40501 atcgcgtaaa actctgctgt tgacaattct tcaaatttct ttacctgata ttccactaag
    40561 caaacgcccc ctgctttttt attcttagca taccgattcg cacacaaaaa agcaaacgaa
    40621 ccttacgaat ctcaggctcg tttgcttttt aaactgcttc agttaaaaat tcgatctcaa
    40681 ctgtccgagt aagtacatct gaatagctgt aagatactcg ttcaaaagaa ttttcttcag
    40741 gatccaaatc aacaacgaat actgaaggat aggtttctgt taaaacgccg cggcgttctg
    40801 tttgacgctt tcttcctgtc tgagcaacca acatgattgg gctgccgata cgaccttcta
    40861 aatctttctt gattgaagct aaagttgttg gcatacagtt cacctctact atcaactata
    40921 ccacatattt ttaaaatttt caagtttacc ataaaaaaaa aagaaatgca ataaaaataa
    40981 ataagaagct cattaagatt tttctaaatt attcctcatt ataaaatttc aagtgttttt
    41041 ttattttgag ttttactctc ccgtaagcgc tcattacttg cttgtgatcc attgctaaac
    41101 attcagccat gtctacaaaa tcaacgcctt gcatataata atgaaaaatt ttagcttcta
    41161 tttttgaaaa ctggatttcg caattttcga ttgcttcttt taataaaaga tgctgaattg
    41221 ctaaaggttc ttgcaatgtg atctctgcta aaaagtctgg tccttctgtc tctatttttt
    41281 ggtcgattga gacttcatct ttttgatcac gacgcttcag cgcgttctgc ttcctcaaca
    41341 gagaatagat ttgattgctt ataacgcttt tataataact tccaaagcta acacctttgc
    41401 ttttttcgta agttctcaat gcctgatata aagcgatcct tgcttcttgt tcccaatcct
    41461 cagaaacata atttcttaaa taataggtgt ttttcaaacg tagtaaaagt ggaaaatatt
    41521 tttgatacag ggcagcaaat gcttcttcgt caccattacg tgcagcgcgc aataactcct
    41581 cagacataaa ataattcctc cataaattcg tttatggagg aatcttatca tcaatgattc
    41641 atcgaactga ttagaacgat atgttattta ttttttctca ttaaatcgtc caatttttgg
    41701 gacaattttt ttaactgctc ttcattccat ggagaattac gtcggaaatc aataaaatgc
    41761 atatctttat tttcttgggc tattgttttt tccgtttttt ttatcgtctt atataattca
    41821 taagcagaag ttcgtaatgc tccttgggaa aagatcatcc actgttctgc taaatcgctt
    41881 gtcgccactg tcacttgtgt caaacggtca ttcagctgac cagcgattct ctcgatataa
    41941 ctgtcagccg tttcgtcttc tttcgtaaaa acaacatcca actggtattt cttataattt
    42001 tgttggatgc ccggaactag ttgggcatcg aagacaacga tgacttccat tccttcatat
    42061 ttcgcataat ttgataaacg atgtaaaaga gcttctcttg catccgccat tttgtcctgc
    42121 tttttaagct ggacaagttc cggccacgaa ccaatcatgt tgtagccgtc tacaatcaac
    42181 agctgttttt tcatggtcta ctccccctga cgttgggaaa aagcctgata cataagcaaa
    42241 ccagcagcaa cactggcatt taagctctgg acgtgcccgg tcatcgggat cgtcagcaat
    42301 tcatccattt cttttttcaa tcctggactc attcctttgc cttcattacc aatgactaaa
    42361 gcaattgatc ctttggcatt ccatttttgg aagtttgtgc cggtcatatc tgtgccaaat
    42421 acccaaaatc ctgtttcttt gagctgttgc acagcttgta ccaaattcgt cacgcgtgca
    42481 acagggacat gttcgactgc cccagtggaa gtctttgtta cgataggtgt gattccaact
    42541 gctcggtgtt ttggtatgat cacaccatcg acgccacttg cgtctgctgt ccgtaaaatc
    42601 gacccaaaat tgtgtggatc ttcgatgcta tccaaaatca aaaagaaggg gtcttctttt
    42661 ttctcttttg ttcgttcaat cagttggtca agggataaat attcataagg tgtaatcgca
    42721 agaacgactc cttgatgcac gccgtgatcg ctcaaagttt ctaatttttg tttaggaacc
    42781 catttgactg gcacagcttg ttcacgtgcg atttctttca gttcttctac tttttctcct
    42841 cgtgaatctt cttgaagaaa gagtttattc cctcgccctt gttgtagcgc ttctactgcg
    42901 gcatggtgcc caaagacgaa attatcttct aatgcttctt cattttgatc agaaactgac
    42961 tgatttcgtc cagtttgctt tttatttgaa tatctatttt gttttttgtc ttcaaatcca
    43021 cgtttcgatt gatgtttgtt tgttctttct ttacccattt ttttcaccaa ctttctgaat
    43081 acaccagcgg atcaattctt ccatccgctc ggtttgtttt gtcaaatgca gatagcccat
    43141 gagcgcttcg aaccctgttg cgacccgata cgtcgtaaca tcagcatttt ttgcgctcgt
    43201 atggctttta gcatttcttc ctctgcgata ataaagttct tcttcttctg tcaaaagatg
    43261 ttcttccagc atttgctgaa tcaaaaaggc ttgtgcattc gcagaaacat aattcgttgc
    43321 cgcacgatgc agcttattgg gttttgtttc tccttgatca atcaggtggt cacgaacata
    43381 gatttcgtaa atcgcatctc caacataagc caaggctaaa ccgttcaatt gtgtgtaatc
    43441 tctcatgctt ctcttctcca tcgggttcct tgcggtgtat cttctaataa gatccctttt
    43501 tctttcaaca aatcacgaat ttcgtcgctt cggacaaaat cacgattgct tcttgcttga
    43561 tttcgttctt cgatcaattg ttcaatctct tcatctaata actcttctgc tgtaaataaa
    43621 ataccgaaga tcgaaagcca ttgcgagaat aaagcttctc cttgtgccaa gacagcggca
    43681 gaaactttcg gctggtctag ataagtgttc agccacttag caagctcata gaccactgtg
    43741 atcccatttg ctgcattaaa atcatcgtcc atttcttcga tgaatcgctg ttctaaattt
    43801 tctaacaaag cgattttatc cgcatcatct gccaaattct ctgaggcagt ttcttggcgg
    43861 aatttcaaat tgtcaaacgt attcttcagt cgttgtaaat tggtggctgc ttctttcatc
    43921 gtcgtttcac tgtagcggat cggacgacgg tattgtgtgg tcgccatgaa gaaacgcaag
    43981 acttgtggat caacgttttt gatcatttca tgtacagtga tgaaattccc tagagattta
    44041 ctcatttttt catcgtcttc gccaatcgtt acatagccat tgtgcatcca gtaattagcg
    44101 aattttttcc ctgttttcgc ttcactttgt gcgatttcat tttcatgatg aggaaattct
    44161 aaatcttgcc cgccaccatg gatatcgatc gtttctccta aatgttttgt tgccataacg
    44221 gaacattcaa tatgccaacc gggacgtcct ttgccccatg gtgaatccca agaaatctcg
    44281 ccaggttttg cttgtttcca taaagcgaaa tctaatgggt cttctttttg ctgctgttct
    44341 tcccctgtac gctgacttgc tccgacttcc agttcatcga tggattggtc acttaatttc
    44401 ccataatttt caaatttacg ggtacggtaa tacacatcac ctgaagattc ataagcatag
    44461 cctttttcaa ccaacacttg aataaatgct aaaatatctg gcatatgatc catgactctt
    44521 ggatgcagtg tagcaggttc gacattcaac gctttcgtat cttcttcaaa tgctcggatg
    44581 aaacgatcag caacttctgg tgctgtaata cctaattcgt ttgctgtacg gatgatttta
    44641 tcgtctacat ccgtgaaatt cgatacgaaa tcgactttat agcctcgata ttcaaaatag
    44701 cgtcgaattg tatcgaaagc aatggtgcta cgagcatttc cgatatggat ataattatac
    44761 acagtcggtc cgcagacata cataccgaca tgtccctttt gtatagggat gaattcttct
    44821 ttttttctgg tcaatgtatt gtagattttt atcattctga tctcctcact ttttctcctt
    44881 tgattcgaac gacttttgct ggtactccta ccgctgtagc gtcgtctgga acatctttca
    44941 acacaacggc acctgcaccg attttggcgt tttttccgat cgttaccggt cctaatatct
    45001 gcgcattcgc agagagcatg gctccttgtt taacagttgg gtgtctttta cccgtttctt
    45061 ttcctgtgcc gcctaatgtt acgccatgga ataaaatcac atcgtcctcg atttctgcgg
    45121 tttcgccaat gacgattccc attccatggt cgatgaacac gccagttcca atcgttgctc
    45181 ctggatggat ctcaacacca gttaggaaac gccagaattg agcattgatc ttcgccaata
    45241 aaaagagccg gtgctgatat aaaaaatgag agaaacgatg ccaaaacaag gcatgaaaac
    45301 cagggtatgt caaaacgatc tcagctgtcg aacgtgcagc cggatcgttt ctctttgctg
    45361 catcaatcgc tcgggtaaac caactcatga ctttccactc ctttcgtaag aattttatgt
    45421 aggaaatcat cttcttataa agaaaagaag cgccttttaa ataagtttcc ttatcaaaag
    45481 acgctttccg cgtggttcca cttttcttca taatcaatga ttatctctga cagctaacgc
    45541 ctgttatacg ttcttggcta ctttgtttcc tataaaacag tttttcctgt tttctgtgtt
    45601 tcgccaagac cactcccaga ggcatttcgg caaccattca ttgatacctt gcaccacccg
    45661 gtatctctct tgtaatgatc agctgcttac ttttttctgt tcatcgtgtt tagttcagta
    45721 cttgatttaa atgtgctaat gatttttcac gaccaagcaa ttcgatcgtt tcaggcaatt
    45781 ctggaccatg catttgtcct gatacagcta cacggattgg catgaaaaga ttttttcctt
    45841 ttacgccagt ttctttttga acagctttga tggcagcttt gacagttgga gcatctacta
    45901 cttccatttg ttctagctgt gctttgaatg cgtggagaac agtaggaact gtttcaccag
    45961 caagtacttc ttttgctgct tcattcaata caggatgttc attgaaaaat agcgtagaca
    46021 gatcaacgat ttctgcagca tagctcattt gaggttgata caggctaacg atcttcttca
    46081 cccattcgat tgtcttttca tctgggtttt ctgctacacg tccatcagcg attagataag
    46141 gcagacacat gtcagtaaat gcatctagat ccattgcttt gatgtaatgg ttgtttaccc
    46201 attgcagttt tttcgcatcg aaagctgcgg gtgatttact taggcgtttt ggatcgaaca
    46261 ttttgatcaa ttcttggtgt tcgaagattt cgtcttcgcc cacaggtgac cagcccaata
    46321 atgcaataaa gttgaacatt gcatctggta gatagcctaa ttcgcgatat tgttcgataa
    46381 attgcaagat tgattcgtca cgtttgctta gttttttacc tgtttcactg ttgatgatca
    46441 atgtcatatg tccgaaggtt ggtggtgtcc agccaaatgc ttcatagatc atcagttgtt
    46501 ttggtgtatt ggcaatatgg tcatctccac gtaaaacgtg agtgattttc atcaaatgat
    46561 catcgaccgc aacagcaaaa ttataagttg gcataccatc gcgtttttgg atgacaaagt
    46621 cgccaccgat attatcggat tcgaacgaaa tagcgccttt caccatatct gtaaatgcgt
    46681 attctgtatt gcgaggcaca cggaaacgta caacagattc tagaccttgt gcttcttttt
    46741 ctgcttgttc ttcaggtgtc aagttcgcac atgttcctgc gtaatgaggc atttctcctc
    46801 ttgcgcgttg tgcttcccgt tctgcttcta attcttcttc tgtgcaatag catttgtatg
    46861 ctcgattgct agcaagcagc tgatcaatca gcggttgata gatttcttta cgttcagatt
    46921 gacggtaagg gccgtattct ccaggatttt caggagattc atcccaattc attcccaacc
    46981 aagcaaggtt ttccaactgg cttttttcgc catcttcgat attacgtttt tggtcagtat
    47041 cctcaatacg aataataaag tctccatcat tgtggcgggc aaacaaataa ttgaataatg
    47101 cagttcttgc atttccaata tgcaggtgcc cagttggact tggtgcatag cgtacgcgta
    47161 ctttcgtcat tttcttcctc ttctttcaaa ctagactttg actaacagca aaaattccgt
    47221 cagttacggc ttctcaaata tcatttattt aaagccactc agcacaaatt ttaccttttc
    47281 taaggtaaat agtaaagcca actggttgaa tttccttacc agttggctga atttcttttt
    47341 tattttgatt gtttagtctt tttctctgtc tcttttttat tgtcgattcc tcgtcctgca
    47401 tgtgcaggtt tagcaaagat catgcgtcca gcagctgttt gaagggcact tgttacaacg
    47461 acttcgatac gttcattcat gtaatgctgt ccatcttcta cgacaaccat cgtgccgtca
    47521 tcaaggtagg ctactccttg ttggcgttct gtcccggctt ttactactag aacattcatc
    47581 gtttcaccag ggatgacgac tggttttacg gcatttgcca acgcattgat attaagaaca
    47641 ggtacatttt ggaattcaga gactttattc aagttatagt cattggttac aatgacacca
    47701 tctaaaagct tagccaattt gatcaatttg ctgtctactt cactgatatc ttcaaagtct
    47761 ccatcgtaca tttcgacaga tataccctct tctttttgca aggcatttag gatatctaat
    47821 ccgcgacgac cacgcacacg tttcaaacta tcccctgagt ccgcgatgta ttgtagttcg
    47881 taaagaacaa aattagggat catcaaggtt ccttctaaga atccagtctt tgtaatatca
    47941 taaatccgac cgtcaatgat cacgcttgta tccaagattt tatatttatg gaaattttct
    48001 cctgattttc gttctaacag ttgactctca acttgactag ctgtttcttg ctcgattttt
    48061 ttgcttcgtg aaccaaaaac tttcttccat tcttcgattc gtgtcgttcc cattctcaat
    48121 cccagatagc ctaagatgat catcagtaaa atcggcaata cactattgac gaatggaatt
    48181 tccatattgt aaagaggggt cgagatcaca acacctaaag tcaatccgat gatcgcaccg
    48241 atcgcaccga agagaagata agtcaagctg acctcactta gtttttgttc tacttgtttg
    48301 ataatagctg agatatgttt ggccaacagc aaagataata aaaagaaaat aagtgcgcct
    48361 aacaggctgt tggtaaatgt attattcagc catccgtttg actgttgacc gattgcctgc
    48421 caagctgagg gtaaaaatga aatacccaag cttgcgccga caatgatcat cagcaaagta
    48481 atgacacgtt tttgcatcgt ttttcctccg tttcgacaat catttattag ttagcggaac
    48541 acacgcttca acgtttcagc caaagttgct actccaacta tctcgattcc ttttggcgct
    48601 tcccagttac ctaagttgtt tttaggtagg tatacttttg tgaagccgag tttttggact
    48661 tctttgactc gttgttcaat atgactgaca cgtctgattt ctccagtcag tccaatttca
    48721 ccgatgaaac attccgttgg ctgtgttcct ttttctttat agcttgaagc aatactgaca
    48781 gcgatagcta aatcaatcgc tggttcattt aattttaccc cacctgcagc ttttaagtaa
    48841 gcatcttgat tctgaagaag caacccagct cttttttcca gcactgccat gatcagagat
    48901 acccgattga aatcaagacc tgttgttgtt cgtttggcat ttccgaacat agtaggtgtg
    48961 actaatgctt ggatctctac caaaattgga cgtgtccctt ccattgccac aacgatagca
    49021 gagccagttg caccgtctag gcgttcttct aagaaaatct gtgaaggatt agcgacttct
    49081 tccaatccat gctcatgcat ttcaaaaatc ccaatctcat tcgttgagcc aaatcggttt
    49141 tttactgctc gcaaaatccg aaaactatga tgttgttcac cttcaaaata taacaccgta
    49201 tccaccatat gttctaacat tcttgggccg gcgatcgagc cttctttggt aacatgaccg
    49261 acgataaaaa tggctatccc atttgtttta gcggttttga gtagttctgc agtggtttct
    49321 cttacttggc tgacacttcc tgccacactt gtgatgtctg gctgcgtcat cgtttggatg
    49381 gaatcaatga taacataatc aggggagata tgttcgatgg cacgactgat ttcgttcata
    49441 tctgtttcag cgtatagata aaattctgtg ttgatacttc cgaggcgttc cgcgcgtaat
    49501 tttatctgct ctgcgctttc ttcgccggat acatataaga cttttccgcc tatggctgct
    49561 aattgctgag aaacttgtaa cagcaaagtg gatttcccga tacctggatc gccaccaatc
    49621 aagaccatag accctggcac cacacctccg cctaacacac ggttcagttc ttctaatttt
    49681 gttttgatcc gaggttcttt tttagggacg acgtctgcga tttttgttgg ttttgctttt
    49741 tctcctgtca aacttgtccg agttctgcgg tccgttgtat cttgttcgat ttcttcgacc
    49801 atcgtattcc attttccgca atttgggcag cgtcccaaga acttaggcga cacatacccg
    49861 caattttggc aaacaaattg tacttttgat ttttttgcca ttgtaccgcc tccttttatc
    49921 ctaatcatga gacattaaat caagcttaag tatctttaac gttttattta ctggaagaac
    49981 caaatccgcc ttgacgttga gcagtcgttt gatcttgatc tgcttttaaa aacggcaaaa
    50041 agatcccttg gccgatacgc tcgccttttt tgatcactac atccttgaaa ccaaagttgg
    50101 tgaattggaa catgatatgg ccttcatttg cctcattatt gtaataatca ctgtcaatca
    50161 cacctactcc gtttgtcaac actaaaaacc gcttcaacgg attgcttgaa cggttagcaa
    50221 gctgcaaaaa ttcatcttct cccatgtaag ctttgattcc agtaggcacg agggttggtt
    50281 tcaattgttt ttcattttct gatacatctt ttttcaatcc atctgcgact gtttccaacc
    50341 cctttttcca gcctgctttc cagatacttg gaatcacgac ctcttctgct gcttcaaaat
    50401 catagccagc agcatgatgt gtagcgcgct gcggtagatt gatattttga tctttatatt
    50461 tagaaataac ttcaaatcct ctttttctca ccggttttcc tccctaaata aactactcct
    50521 attctaacat atccgaggct gtaaaagact ttctttacaa attcttcaag aaattctgct
    50581 atcttaaagt aaaccattaa ttcgttcttt ttccaaacga ctgcaagttg tacaaaaaaa
    50641 ctatttttta catgacttta ctcgtcaaaa caagtagaat agaacgaaat taatgagtgg
    50701 atatttgatt gaggtgtcac atgcgtaaag aaattaaaaa tgcaaaacga attattgtca
    50761 aagtaggaac aagtacattg atttatccca atggaaatat caatcttgcg gcaatcgatc
    50821 agttagcttt tgtcctttct gatcttagaa atcggggaaa ggaagtgatc ttagtctcat
    50881 caggagctgt aggcgttggt atgcataaat tacatttgtc agaacgtccg actttgattc
    50941 ctgaacagca agctgcggcg gctgttggtc aagccgaact gatgaatatc tataatcaaa
    51001 gattcagtgt atataatcaa cagacggcac agatcctcgt gacaagagac gtggtagatt
    51061 atcctgaaag tcgtaaaaat gtgaggaata cattggaaca gcttttgatt atggacacga
    51121 tccctatcgt gaacgaaaac gatactgtag cagtggatga attagatcat ctgacaaaat
    51181 ttggcgataa tgatcagctg tcagccatca tggcaaaact tgtcgcggca gaccttttga
    51241 tcgtcctttc tgatatcgac gggttttatt cagataatcc gactacaaat cccgaggctc
    51301 agatgtatcg ctatatcgat aaaatcgatg aaaagattct ggcacaagca ggtggtgctg
    51361 gtagcacatt tggaacaggc ggtatgcaga gtaaattaaa agccgcttct aggatatttg
    51421 aagcaaacag cgcaatgatt ttagcaaatg gaaaaaatcc ggcaattatt tttgatattc
    51481 ttgcaggaaa agaagtaggt actttattta aggaggactt gaaatgactg acttgatgca
    51541 attaggacaa caggcaaaag atagcgcgca acaactagca cttatgccta caaaccgcaa
    51601 aaatgagcta cttgttcaaa tggcacaaac catcaaaaag aatcaacaag cgatcattga
    51661 agcaaatcat aaagacttag aaaaagcagt cgaaaacaat atcagtgaaa cgatgcaaga
    51721 ccgcttgcag ctgacacctg agcgaatcaa tgccatggct acagagatcg aaaaaattgc
    51781 ttcgttagac gatcctattg gaaaagtaga tgagatgtgg acgaacaccg acggattgag
    51841 aatcggcaaa aagcgcgtgc cactaggcgt gatcggcatc atttacgaat ctcgtccaaa
    51901 tgtcacgaca gatgcagcta gtctagcatt caaatcaggg aatgccgtta tccttcgcgg
    51961 tggcaaagaa gcattcttct cgaatcaatt attagtccaa ttattgcagc aagtactggt
    52021 cagtgctggc gaatctcctt atgccatcca gtttgtcgat gatacttctc atgaaactgc
    52081 aggacagttg atgcgattga cagaatactt agatgtgctg attcctcgag gcggagcaaa
    52141 attaatccaa cgagtcaaag aacaggcaac ggtacctatc attgaaacag gtacaggaaa
    52201 taatcacgta tatatcgata aagaagcaca gttatctatg gcagtgaaca tcatcgtcaa
    52261 tgcaaaagct tcccgtccat ctgtctgtaa cgcggcagaa acattgctga tccattctga
    52321 gatcgcacct tttttcttgc cagctattga aaaagaactg gtagaacacg gggtctcttt
    52381 acgtgcggat gctcgtgcgt tagaatatct agagacggct gctttagcag aagacacaga
    52441 ttgggatact gaatatctag attatatcct tgctgtcaaa gtggtcgatt ctttggatga
    52501 agcaattgca catatcaatc ggcataatac taaacattca gaaacgatcg tgacagatag
    52561 ttatgcggct agtcaacgtt tcttaaatga agtggacgcg gcggctgtct atgtcaatgc
    52621 ctccactcgt tttacagacg gatcggtatt tggttttggt gcagagattg gtatctctac
    52681 tcaaaagttg catgcccgtg gtccaatggg gttaaatgaa ttaacttcca caaaatatat
    52741 catttatggc gatggacaaa tccgcgaata atgtttcgtg tgaaacatta ttcccgcctt
    52801 ttgccttttt tctgtaaagg ttctatccaa ctagaaaggt gtcaatatgt ttgaaatttt
    52861 aatgattttt tttatttacc ttatttctct gaatatcgct gcttttctgg gagtcggtat
    52921 tttatccctt ttctttcaat tcaaaaaacg aagcataggg agtcaacggg aaaaatggtc
    52981 gcagtatttt gacaaaatcg ggcaaaaagg gctggttaca cgcttgcaca ttagttacat
    53041 ggttgcatta tgcttattag cgacaatcaa ttactattct tttttcgatc attcaatcgc
    53101 ttatacgatt acgctactga ttgctgggat ttttcactta tcttataaat atcagctgaa
    53161 taaaaaccac ttgaatcgga ctttccgcta aaaaatatcc gtacgataag tagagtgcct
    53221 tgaagatcgg caaaaaactg ccggactttt tagaggctgt gacaaagatt ttgtcacagc
    53281 ctctttttcc atataaacgg cgttcgccta ccagctgcct ggtaaatctg tttcctcttc
    53341 gttaagaagc gaaaatccgt caaatgtttc gccatcataa taacgaagca gctcatccat
    53401 ggacaagttc atcgtttttt ctttttcttc ttccgctgat tcagccagta gctgttcccg
    53461 atacacctga tacataccag ctattttttg ctgcatcgct tttctagttt caccttccgc
    53521 attgattatt ggcaattctt tgatatagcc acgataaatc aaccgatcat ctgaaaattc
    53581 aatgggtaat tcaacgagtg tcaacccgac ttcctgaaaa tattcttcca gcatctatta
    53641 tccccccatt cttttttctt aaatgaattt tagcttacct actgaaaatt ttccagtttc
    53701 attattttga gaaaaaaagg gaatgtgaca aaaatcgtgt cacatcccct ttttccgaat
    53761 aaatggtgtt cttctgccaa ctcctcaatc ataagtcaca atattcaaca aacatgagaa
    53821 actgtttttt gaaattgttc cttgtgattc agagttaaac gacagtctca caacctcttg
    53881 gtaaacggcg ttcaatcagc tgaccctcgg tattagctca aaaaacggta aaatatgaga
    53941 agctattttt ccgttttgtt tctctaatac ttaggataaa acgctgattt tacaacctct
    54001 gttaaacggt gttcaatcag ctgtccttcg gtattagctc aaaaaacggc aaaatatgag
    54061 gaactatttt tccgtttgtt tctctaatac tcaggacaaa acgctgattt tacaacctcc
    54121 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
    54181 gagaagctat ttttgtttgg ctgttcttat gacttgatgc aggacagctt tttcacaacc
    54241 tctgatttct tattcttctt catttgtagc aaaataatta cggtgataaa tgatacaaca
    54301 tacactgata agaacaatgg cacctgcaaa ataaaacgca tagctttcac caatatgttc
    54361 accaatcaca cctgcaataa atggtgctaa agctccaccg aaaaagcgga caaaactata
    54421 gctggacgaa gcaacgtttc gttgcagacc tggtatttcc atagcaatcg tcgtcaacag
    54481 tgtattgata attccttgga aaaatcctgc taacacaatg ctgatcggta caaggactgg
    54541 catagaagtt ccagctccta ataacagcaa acaaataatg aataagacaa atgctgctaa
    54601 aattgtccga taagtcgtaa acttccgctc aagagccggt gcaacgaaaa tcgaagcaat
    54661 agccagaagc acaccccagc caaaaaatac aaaaccgact tctaattcag aatagccaat
    54721 caataaaaat ggcgcatacg caagtaaagt aaagaatccg aaattatata acaatgcgat
    54781 tagtccaact gaacgaagtt tatgattgct taacgctttg acaccttccc aaaggcctac
    54841 ttttctcttt ggtttctcag tttcaggtaa aagtaccgct agggctataa aaccgcaaaa
    54901 catcaaaaat gctaccccaa aaaatggata acgccatgaa ccactgccca agaccccacc
    54961 gactagaggg ccaacagcca ttccgcagcc aagtgctgct tcatacatca tgattgcttt
    55021 ttctgtgtta ccaactaaaa tactgacgat cgttgctaaa gcagtagaga caaataaagc
    55081 attcccgaca ccccagccgg cacgtaaccc aatcagcatt ccgatattcc cagataatcc
    55141 gccaagacca gcgaatacaa cgatgataaa caatcctgtc atcaatgttt ttttcaaacc
    55201 gatccgacta gataagaaac ctgtaaatag catcaccaca ccagtgacta acatgtaact
    55261 agtaaataac agggtcgttt cagctggcgt agcgtttaac tgtgcagcaa tcgtttttaa
    55321 gattgggtct actagaccca ctcccataaa agcgatcata caggtagctg ctacaactag
    55381 cgctgaactt ttcccctttg tttcttcatt tgtttgttct tgtatatcta tttgattctc
    55441 caatatttcc atccctttct ctctattagc atcatacatg atttatttta catgtcaata
    55501 tgcacatgta aaacgtggaa actttactgt gatataataa agaaaataag acgagaggta
    55561 ggatttttat gaatacttta gcctatatat tattatgcgt gcttttatcc aaaccacgtt
    55621 ctgggtatga attgaagcag ctgatcacaa ttttttggga agcccatcac agtcagatct
    55681 atacaacgct tgccaagtta gaccagcagg gttatgtgac cgttttggac aaagacgagc
    55741 actctcaaaa aaagatctac cacttgactg atgaaggacg tgctcttgta gaggaatgga
    55801 tcaaagagga aactcccgct cctacgcaaa aagatgaatt tctagcaaaa atctatgctt
    55861 tttcgacgct cgataaaaca acagctactg gactgctatt cactagagaa caacagctga
    55921 acaaaatcat gcaaaaaaat caaaaaaaat tgaatgacct ctcgctagac aagaaacaag
    55981 aatttggacg ttatgttgtg atccagcgca gaattatgct ttgtcagcag gaactccaat
    56041 ggtgtcagca agtaagagaa caaatgcagc agttttttga gtaagggata aacagaacct
    56101 ttctagaaat atctattgaa gagcaaatta tcttcaataa aagagcaata cagttggaaa
    56161 agaaaaagcg atccgaacga caggccaaaa cctatcgttc ggatcactat ttttctagaa
    56221 ctgcattcaa gagactatga caaaaagcat gccaaggcct tttttcaaat aacagtgttc
    56281 catcattcat tactttgaat tgcttttagc acacttcttg aaagcgggcc aacgatgatc
    56341 aactgcaacg gtaaagcggc aatcacattc attttccaag cattcatata agcagagaat
    56401 aattgatcag gtattccttg ttccatgatc acaccgaata aagacataca tgtcaccatt
    56461 cctaaaacca tcaagcatga aatagttaag atcatttgga ccttgctttc tggatttact
    56521 ggtagtttaa aggcaatttt tttcgccagc acaccgatga tgaagacatc caaaatgaaa
    56581 gccaccacga atccaggcac tagaccaatc aacaaacttg ataaatgaag atcttgatgc
    56641 agccataaat tatacgcgct catccccaag accatcaaaa aacacatcat tgttgtaaaa
    56701 attattcctt cttttttatt tgtaggcaat ctgtaccact cctttaaatt tgtcacgaaa
    56761 atcatcgtag cacatttaaa aatttctcgt aagtactttt tttattttct gcccttttat
    56821 ttcataaact tcatcagccg tacgctcgac aaagtaagaa tcatgagaag taaacaaaat
    56881 cgttcccgga tatatttgca ttagcttctc taaagcttcg atcgttgcca tatcaataaa
    56941 attagtcggt tcatctaaca gcaacacatt acttggctta gtaaaaagca aagcaatcgt
    57001 cagacgggtc gcttctcccc cacttaatgt acataacggt ttcgtcaaag tttcctgagc
    57061 aaaccctaag tgattcagca aactacggac aagcgattct gaaaactctg tttgtctcat
    57121 gacaaaacga atgatggatt cttcagatgt catttgataa cccttttgct gatatacttg
    57181 aaagcttact ttcggagaga gaaggattcc ttctccttgt ttgcggatat gttctaataa
    57241 ggttgtctta cctgaaccat tttcaccgac aatcgcgatt tttttaccta aagaaaactg
    57301 aaaatcacca tttaccagta atgttctccc acttctttcc aattggacat tttgtcccat
    57361 gatgggatag cggctgtgga gttcaagaga ttttggtact ggaaaacgga tttgtttcat
    57421 ctgctgcggc ttggttgttt ctcctatttt ttggagtctt ctctctaatg ttttcgcatt
    57481 cttttggatg gctttttgta cactgtcttt ttgtttagag gaagacaacc gatccagtct
    57541 gatttgttgc tttttctttt tactggacac tttttccaac ttttccgctt gttttttctt
    57601 ttcttggatc gatttttcca atcgtttttt ctctttcatg atcgactctg cttctcgtag
    57661 ctgagtcttt ttctccaatt ccttttgagt gagataggca ctataattcc ccgaaaactc
    57721 tcggatttct ccgtctttta cttcccagat tttcgatgct agctcatcta gaaaatagcg
    57781 atcatgacta acaaaaacca gcgtaccata ataataacga agctcttcaa tcagtcgatc
    57841 aatacttttt ctatctagat gggttgttgg ctcgtctaga agcatccctt gttcataaga
    57901 agaaagaaga cgtatcaact cgatctttcg ttcttcccct ccgctccatt tccgctctga
    57961 agtttcttcc agtcttaact ggaaatgact ggctaattcc aaatttaggt cctttgcttc
    58021 agcgagatag gttaattgag gcaggtagtt gaaggtgatc tctgtttgga ttttcccatg
    58081 atcgggaaaa agttcaccgg ctattagttt gagcaaagtc gatttaccga caccattttc
    58141 tcctatgatc cctatacgat cttgctgata gacacgaaga gaatcaatag atagttctag
    58201 ctggttgcca aagctgactt gtagatttgt tatatttact gctaaatttt ccatatgttt
    58261 tacactccta tttcatgtaa aacaacaatt atcgatacta aaaaagagca gaaaaatctg
    58321 tttgcaaaaa aactgcatca catcttttcc tactctgaac gaaataacta tgactaaatc
    58381 accaaatctg tcatagcttt cacctattct actgtcgttc gaaaaaatgg acatactcca
    58441 tccatgaaat tgttgtttat acatgtcttt tgaatttatt tagttgttat tcaaaagttc
    58501 gaaacgtaat ttcatcgatg cagtcatttt tccgacctcc ttttttatta ggttaccaca
    58561 cacgattcaa aaaaagcaat agctactgaa tagtaactga aaacctttta ttcgaacata
    58621 gtgatctttt ctggtgctaa acgaacagac tcgtaacctt cttctaccgc ttttcctatt
    58681 gataaaataa taactggaac ataacggtct ttatccaaac caaatgcctc tgccaactga
    58741 tctgcttcga atccaccaat tggatttgtt tcatagccat gatcacgcgc aactaacatg
    58801 aattgcatag ccgctaagct agaatcgact ttgactacat cgttcatttc ttctcttgaa
    58861 aaactcttgt aataaggaat gatcgcagct aattgttgat ctctgacttc ttttggcatc
    58921 ttccctgatt cgtaagcttg gttgtagatt tcttcgccat attcgtaaca ttgcatatcg
    58981 ccaaaaatca tgaccattgc agaagaagta tcattttgtc tcgtattgaa acggatcaaa
    59041 ggacgcaaga tttcttttgc ttcctcgctt tcagcgacca caaagcgcca tggtttcata
    59101 ttgacagaag aaggtgcagt cgtcgcttct ttgatcattt ccagcatttc ttcgtgtggg
    59161 attttgtatg tttcatcata tacgcgaacg gatcttctac cagtcataat tgttgaaaaa
    59221 tcattttttt gttccattat tattaacctc tttcgtttct gttgttatat tgcaaagttg
    59281 ggtattcaat ttatctaaaa gtgttagaaa ttgtttcatt tctacatcgc tgaaagccgg
    59341 ggatagcacg gaacctgtat ctttatggtc catttcacaa tgcttgattt tcttctgggc
    59401 tttttctgtt agcgctacat acacttcccg gttgttctga ggatttctct gacgcgaaac
    59461 atagccattt gcttccaata ccttcagatg acgagtaact gctccttgat caatctgcaa
    59521 cttttcttgc aaactgcttt gtagcaatgg attttcttct ttcagtctca tcatgatctc
    59581 gtatctagta agacttatac cgatattttc ttcaaagatc tggacgattt tttgatcggc
    59641 taatttgatt tgatataaaa ggtgactaat ctcttctaaa gacataattt cctcctttct
    59701 aataattgac tcgtcaacga ttgacttgtc aataatagaa taaaaaaaga aaaaagtcaa
    59761 agaatcagct gtgtaataaa aaaaacgctc tcaatagaaa agtatagaga aaagaaattt
    59821 tttgtagaat gaacggaaaa aatgataaag aaagcacaac gctaaaacca agattattga
    59881 ttcttatcaa actatagaag atagctcttc tgcagtagat aattttacac cgccaaaaat
    59941 ttccgaaatc gccttttgca agaatcccgt cgaatattag aaattggatc agtatgctta
    60001 aacagtaata aaaaagttat gacaagatgt tgtcacaacc ttttttgagt aaacggtgtt
    60061 caaaaacttg catctgcggt aataagttca accaaacaaa aaacatgaaa atctgttttt
    60121 gtttggctgt tcttactact tgatgcaggg cagctttttc actacctctt tttaataacc
    60181 ataaaatcct attccttcat atctccagcc catcggtgtt agccaatctc tttccttagc
    60241 actcaaggta tagtgatggg taccagtttt ttcatttgga ttaaatagtc gataaattgg
    60301 cttggaacca ccagaataaa aacttagacc ttcataacgc caaccgattt ttgttaaaga
    60361 gttgatctca gattgtgcca ttgtgtaatg atggtctcca ctgtaaggat tgtataaacg
    60421 gtagacaggt tgtccgctgt ttggtgcttg ccaagcaatc ccttcatatt cccagcctac
    60481 agttggcaga taattttttt ctccggcatt caatgtatat aaatgttctc ctgtatgacg
    60541 gttatacaga cgatacatcg gagaagttga agttggtgct gtatcatact gtgtcagatt
    60601 ataggtttcg attacattgt tcaatgtaga accataatta ggcgcagtgg cataacgacc
    60661 tgttagccaa gccgtagcat ctttgtaaga gttcgtattg cttttccatg caccggaata
    60721 gtagtaaacg cctggctgaa aagaagtcgt cttcaatacc gttacgttat cttgcaatga
    60781 ttctttgtac gaaggatatt ttctaaaagc ggcattcacc gtaatccatt gcccattcac
    60841 atattccgaa gtaggcatcg taacagattg gccattatag cttcctttaa tcccaaatag
    60901 attgtaatta ggggcagaag ccaaagtact gttcccccag ccactttcaa cgatggcttg
    60961 agcaatcatg actgatgcat ataagtcatt ggcacttgcc aaactttgtg cagttgaggc
    61021 aatactatca atgaacgctt gttgagaatt agcagatgag cgagcataaa aagcatagcc
    61081 agtatagttt tcgaattcag acaaatctgc ttgatgggta tgtgtcaatg tttgcccatc
    61141 tgtcaattgg ttcgtgattt cttctgcatt ctctggattg ttctgagctt ctgctgatgt
    61201 actgtcttcg gtttgtgttt gttctgttgt ttcagttgtt ggttcttcgg ttgaaattgt
    61261 attttctgag acagtagctg actgcaaagt actttctgta tcgttttcaa ttcctgtttc
    61321 tgaagaactc gatgtctgag ttgcgacagt ttctgtcgat tcttcagcta gcactgttgt
    61381 gttggcaact gctagatttc ctaaaagaat aacgctaaat aataaattct ttgctttcat
    61441 cttttttctc ctttgaatta tgaaactttt cctaaataac agtgtaacta aaaaagtaaa
    61501 agcaagtcaa tgtccatatt taaataacat ttgtttcttt ttaataaatt tgctttttct
    61561 gcataaaaag acgtaaatga agaatattct tctccattca cgccttaaat tgatggtcgt
    61621 ttgaataact atttaacaag taattctctc tggatattat ccgttatgga ttatacttcc
    61681 tgtgtcgtcg gacggataat catgttgctg acagacatcc ggtctggcgt ttctatcgca
    61741 aatgctacag cagaagctat atcttcagat gttaatcccc attctttttg cgcttcatga
    61801 agttcaagag cagccttttt gtcgctgatg gtttgatata attcagtttg aacagctcct
    61861 ggagagatga tcgtagattt gatgttattt tgccgttgct cttgacgtaa tccttccatg
    61921 attgcacgga cagcaaattt tgttccgcaa taaacggcgg aatcaggata aacaacatgt
    61981 cccgccacag aatcagttga aatgatatgc ccagattttt gtttttccat gattggaagt
    62041 acggcggaaa ttccatttaa cacacccatg atgttgatat caagcatatt ttgccattca
    62101 tcacggtgac cttcaactaa tggagcggtt ggcatgattc ctgcattatt gtacaataca
    62161 tcgattcgac cgaacttttc catcgctaaa tcgataactg cttggacttg tgcaaaatct
    62221 cttacatctg ctgtttgaat atatagctca gcttctggta aagattcttt tattgctttt
    62281 aggcgatctt ctcggcgtgc agctataact agtttagccc ctttttctgc taatagcttc
    62341 gttgttgctt caccgattcc gcttgaagcg cccatgatga caatcacttt ttctgataaa
    62401 tcattcattc tattctcctc ctgttttgaa aaatctcttg cttatactca ctatctctta
    62461 tttcatctta ctgttcaaat aatccgttcg acaggtttga cgattttggc agggattcct
    62521 gcaacgatgg tattgtctgg gacatctttc gtaacagttg cattcgctgc aacaatggca
    62581 ttctcgccaa tcgttacgcc tggcagaata gttgctcctg ctccgatcca agcatttttc
    62641 ttgatgtgga ttggctttac tgacaagcct cttcgttctt ttggatcggt tagatggttc
    62701 accgtgatga ggttgacacg aggaccaatc aatactgaat cttctaaaca gatgccacct
    62761 aagtcaacga acatcacttg acgattgata aagacatttt tccctatact aatatgtttg
    62821 ccgaaatctg tataaaaagg cagcagaaca gtcaccgtat catctacttt gtcactaatg
    62881 atattttcta gccgtttgcg gatatctgct ggttcatggt acccagtatt cagcgctgct
    62941 aaaggctctt cgttttccgc ttggatctga tggatcactt caaagagcgg atccgttact
    63001 cgaatctctt tattgacgat ttgtttttcg atgaggcttg tcattttgct ccctcctttt
    63061 atccttactt tataacggac ggcttatgat gtctaatact tataaaacat acttaactat
    63121 tccttttaac tatacttagt ggtatgcttt tttaaaaaca ggagggaaaa aatggatatt
    63181 cgtatactga cgtatttttt agcagtagct agagaaaaaa atatcagccg agcagcaaaa
    63241 tctctgcata tcacgcaacc tactctctca aaacaactga aagatttaga agaagaactt
    63301 ggtgtgacac ttttcactcg aggctcccga gagatacagt tgacggattc tggtcgatat
    63361 ctttattcaa aaggaaaaga aatcttgtcg ttggtagaaa aaacggcgaa caatttaaca
    63421 aatgacgcaa tcatctctgg agaaatctac attggcggag gcgaaactaa agcaatctct
    63481 ttcatttctg atagccttca ttcgttgatc aaggcgcatc ctgatatcca gtttcattta
    63541 tatagcggga atgcagatga tatcatagac aaactggata gcggattact tgattttgga
    63601 ctcgtaatcg aaccgacaaa caaacaaaaa taccagtcat ttcgactgcc agcccaagat
    63661 cgttggggac ttttgataca tcggaatcat cctttagcaa agcaaacagt catccatccg
    63721 caggaactga aaactgttcc tttgatcatt tcccggcaaa cgtcagtgga cagtcaacta
    63781 gctgaatggc taggtttcag catagatcag ttatcgatcg tcggaacata taacttattg
    63841 tacaatgctt ctttgatggt cgataacggg acttttggtg cattagctct agatgggatc
    63901 atcaatactt atgggacgga aaaagtattc atcccactcg atccgccatt acaagcttca
    63961 ctgaatctca tttggaaaaa agaccagcct ctttctcctg ctgctcgtaa atttttagat
    64021 gtcctaacgg agaaatacag ttaagtagag tttttttcct tcgctttcag ctttttaaca
    64081 acgaataagt taagcaaaag atccaatgat taaataacat aagtttctta atttgttcct
    64141 tccttttatt tctgataatt ccagtaaatt actataatta taaaaaaaga attgacaaaa
    64201 cataactgtc tatataaaat tgattacgtt aacaataatt cgtttcatgt gactagatca
    64261 tactaggcat gaaaaatctc tgaggaacat ggtttcccta ggaggttttt cgtgtctatt
    64321 tttgtttggc cgcaatcaac tggtctagca aacgtgaaaa ccaaatcttc aaaaagggtg
    64381 cgcgcatgaa aatcaaaaag atctttaatc aaaatgcagt attggtagat gacggtggac
    64441 aggaaaaagt agctattgga aaagggattg gatttgacag aaaaagaaat gatttgattt
    64501 ttgatcgtga tgtcgaacgg atatttgtca tggaaccaga aggtcaaatc aagttgcaaa
    64561 atttacttaa tcagattgat gaacgctttt tatttgcagc agaaacgatc attgatcatg
    64621 cggaaactgt tttgatggaa aagttgaatg aacatgtatt gatcgctttg accgaccaca
    64681 ttgctttttc tgcggagaac attaataacg gtatcgtcat ccgcaacaaa cttttaagag
    64741 aaatcgaagt gctttacagt gatgaattca gtattgctca atgggcagta gagtatctga
    64801 caaaagagct tgggattcca tatacttatg acgaagcagg atatatcgca atccatattc
    64861 acagcggacg aagcggtcgg aacaataacc atcgcagtat ccgtgaagtg acgattgtat
    64921 ctgacatcat ccatttagtg gaatcagaat tggaaacaga tatccattca gaaccatttt
    64981 cactgaatta ttcaagactg gtgaatcatt tacgactgct tctacaacga acccatgcgc
    65041 aacaatatgc tgttttagac acggaaatcg tagaaatggt caaacgaaaa tacccagaaa
    65101 gctataagat ttctaaaaaa atccgtgtgt tgctgaccaa agaataccag ctcgcgatca
    65161 cgaaagaaga actgggttac ttggccatcc atatcgagcg tttaagaagc gcaatcgtac
    65221 aaacaaatgt taacaatcat gaggaggaaa aaaattgaaa gcatatctac aacgaatggg
    65281 acgttccctt caacttccag ttgcggtctt acctgctgct gctttactag tagggatcgg
    65341 aaactggtgg gcatcttata gtaacgacat tgtcgctcat tttttacaag ctggcggtaa
    65401 cgcagtttta ggtcaattac ctttgctgtt tgccgttggg ttagcactag ggatgtcagt
    65461 ggacaaatca ggtgctgctg ccttggcagg tttggtcgca tttgaagttc cagtaaatgt
    65521 tttaaaaact gattcagtag ctactttatt gaatatcaaa ccagaatcag ttgatccatc
    65581 ttttgctcag atcagtaacg tatttattgg tattattgcc ggattgattg cggcagctct
    65641 ttataatcgt ttccacgaag caaaattacc gatggcttta tctttcttta gcggcaagcg
    65701 tctagtgcca atcttggctg cctttgtcat gttgctgatc agtgcagtgc tactgctcgt
    65761 atggcctccg gtttactctg ctttagtttc ttttgctaaa ttcattttga gtttaggctg
    65821 gggtggtgca ggtctttatg gcttcttcaa ccgtttactg atccctacag gattgcatca
    65881 agcaatgaac tccgtgttct ggtttgacgt agcaggaatc aatgatattg gtaaattttt
    65941 ggcttctgaa ggaacaaaag gcgtaacagg aatgtatcaa gctggatttt tcccaatcat
    66001 gatgttcggg cttccagcag gagcatatgc tatttacaga aatgcacgtc cagaaaacaa
    66061 agcaaaaaca gcttccttga tgttagcagc agcttttgct tcattcttta ctggtgtaac
    66121 agaaccactt gaattctcat ttatgttcgt tgcttggcca ttatatgtct tgcatgcaat
    66181 tttcactggg atttctttag cagtgagtgc attcttccat tggacagcag gatttgcctt
    66241 tagtgccgga tttgtcgatt acttcctatc tttgaaaaat ccgattgcga atcagccatt
    66301 gatgttgatc gtccaaggat tagtaacagc agtgatttac tactttggct ttaactttgc
    66361 tatcaaaaaa ttccatctga tgactcctgg ccgcgaggaa gcagatcttt cagatgacga
    66421 tactgcaacg actaattcag acaataaata tgcggcacaa gcaagcaaaa tctacgctgc
    66481 attaggcgga gccgacaatg taacttcgat cgataactgt actacacgcc ttcgtttgca
    66541 agtcaaagat acaggcctga tcgatcaaaa taaaatcaaa gccactggcg tacctggtat
    66601 gaagatcatc gacggaaaaa atgcgcaagt catcgtaggt acagaagtac aattcgttgc
    66661 agatgagatg gcaaaactac atggtggtgc tgcaacacga cctgcacaaa caaacaccgt
    66721 ggttaaaaca gaaacatttg ccggtggaac gatcgaacaa gatatttacg ctatcgcaaa
    66781 tgggaaactg attccaatca ctgaagtttc agatgatgta ttcgcggaaa aaatgatggg
    66841 tgacggctat gctgtattac cagaaaatgg agaaatcttc tctccaatcg ctggtacgat
    66901 cacaaacatt ttcccaacga aacatgctgt cggaatccaa acagatgctg gtatcgaagt
    66961 cttgcttcat atgggcatca acactgttga cttaaaaggc gaaccattca ctttatatgt
    67021 aaaagaaggt caaaaagtcg caagaggtca attgattgct ttagttgatc ttgccgctat
    67081 tcaatctgca ggcaaaaaca cggatatggt cgttgtattc acaaatggcg acaaagtcca
    67141 aagcttagaa atcgaacccg cacgtgatgt aaaagcaaat gataaaatcg gctctgtttc
    67201 caacaaagcc tgagccatcg tcttccccag acttttttat cgataaacaa acaggagctg
    67261 agacaaaaat cttgtctcag ctcctttttc cgaataaaaa cggcgttctc ctgccaactc
    67321 cgcggtaata agtccagcca aacgtaacat atgaagagct attttttatt tgactcttct
    67381 tattacttga tgcagaacag cttttttaca acctctgata aacggtgttc aaaaaccagt
    67441 ttatcggtat gaagtcaaat ttatgcaaaa atgtggaaag ccattttcac aaaatttttc
    67501 ttcatactca aaactaatcg gctttttcac aacctcttta tacattattc ctgatccaat
    67561 atttctttta atgctgcttc aagagaagtt aatggttttc ccaatacttt ttctaaatca
    67621 tcagaggaaa tagctaactg atccttttta atatcatgtt ggatcgctaa aaacatgtca
    67681 gctgcttctt gagggaatcc ggcttctttt agattttcga caaacgctgc atcacttgct
    67741 tcaataattt ctaacttttt acctgtcgca cgctctaatg ccttggctaa ttcttcatag
    67801 tcagttagat tgccggataa ttctaagatt tctggagagt cactttctac tagtactttt
    67861 gctgcagctt ctgcatattc acgtttcaat gcccaacctg tttttccatt tcctgcagca
    67921 taaacgaatt gaccagtttt taaagcctgc ccaatgatag gtaattcatt ctctacatac
    67981 caattattcc gtaaaaatgt atgggaaata cctgaatcct taatcatttt ttcagtgaat
    68041 tggtgatctg gcgctaatac actgatcgag tgatcggcat ctgcaaaact tgtataagcg
    68101 atataagata ccccagctgc ttgtgctgcc ttcactacat tcttatgttc ggcttgacga
    68161 tttcctggaa cgccagagac aaaaagcaat cgatctattc ctttcaacgc ttctttcatt
    68221 gagtctaaat catcataatc accaatacgt atattaaatc cagcttcttt cagtgcttct
    68281 cctttttctt cactacgtac tagtacatag atttcatttt gtggaaccat cttttttaag
    68341 aattccagcg cataaccacc aaaacctcca gttgctcctg ttactaaata atgcatattt
    68401 gccactccct attcttttta tttgttaaga tttacagctg tacatttata aattacacct
    68461 ttatcataaa gagtgtggta agataagtca agcaaaaaaa atgaaaggat gacaactgat
    68521 gaagtattct ctgcaattta gtgatgcaat ccatgtactt gcctatattg aaatgtttca
    68581 agatacccga tcactttcta gtgagatgat tgccagtagt atcgaaacaa atgcagcaaa
    68641 tgtacgtaaa atcatgagtc agctcaaaaa atccggtttg atctttacaa aaaccggaaa
    68701 accgcagcct tcccttgcca aaagtccaga tcagatttct ttactagatg tctatcgcag
    68761 catcgaaggt aatacacagc ttatccaagt cgatccaaaa acaaatccaa attgcgtcgt
    68821 tggtgcgaat atccaagaag ctttagaaga aagctacgct cggctgcaga aaaaggcaga
    68881 agaagaaatg gctaagatca cacttgatag tctgatacat aaaatagccg ttttggaaaa
    68941 agaaaaaaga ccagaaaaca caggcctcgt agagagattt ttataattct ctctactgtg
    69001 cctgcatttt tctgatcgga atcgtctaca taaattcttt tattctccgg gggacaaaag
    69061 ccttgtcatg cccctttttc ctctctaatg ctcaggtcaa agcgctgaag tcatgacgtc
    69121 ttgataaacg gtgttcaagt agctgaccct cggcattagt tctgaaaaat gcaaaatatg
    69181 gaaagctatt tcccattttt cctctctaat gctcaggtca aagcgctact ttcacgacct
    69241 ctatattatc atggtttatt gactaccggt atccagattt ctcccatata tttcccgttg
    69301 gtaactccca tttcgactac agcatttgga cccccaacat aggcgtaatc ttctacttct
    69361 cttaaaacat gcccaaaggc cgtattcgtc aaatcatttc ttaattcttc aggcgattct
    69421 gctaaagcga ttacaacaag atacgttcct tttgggaatg ggatgattcg ggtagtgttt
    69481 ggaacatcct gactcgtttg aacaccgaca tagtacatca ttttcccgtt atagggttcg
    69541 ttgactgtaa agaaataatg attttcagca gcttgtttca atgcatctat tgttccatta
    69601 ccgataactt tttgctcaaa tgcttgtttc tctgcagcca aaccgacaaa atccgtattg
    69661 ctactcttca attccacacc aatacccgtc accgtaaaat ctggtttttc ttcgatccta
    69721 tactttgcca tttctcccac cctttctatt atattaagtt catcttacat aaaattaaag
    69781 ggaaatgaca cgattacgct cttttttcta gggatatttt tattttttaa ctattttttt
    69841 gctttcactg caatccaaat ctcactgtaa acattactga aatcctctcc ccattttgta
    69901 aaagagatct cgggagcttc gactaactca taatctgaag aaggaagcca ttctgcatag
    69961 attttccccc aagtatcttg taaagtttgg ggaaatggcc ctttattagg aaaaatggcc
    70021 caagtgtgtt cttctatagc aagcatttct aaatcagcgt acggattttc ttttgtcgta
    70081 gcaaagccaa tcatgtgcat caaactgccc ttttcttcca agcgggctcc atcaaatcca
    70141 taagaaacgt tgattacttg atttggatat aaatctccta acttatgcat ctcttccctt
    70201 tgagtctccg tgatcgtctg cgctaacttt tgtatttctt gattgactcc ttcaaatttg
    70261 acaggaactt ttttcgccac acctgccaca taaaactgtt ctttcttttc gatacgaaat
    70321 tccatcgatt ttcctcctct gacatcaata aaaaatgtca agcgtggaaa agatttttgt
    70381 atgccgtatc tgctgatttc tgaaggcaga tatcctgacc aatcacgaaa agctcttgaa
    70441 aaaccttcga ctgtttcata tccatatcta aacgccgttt ctgtcactct tgatccatct
    70501 aggagatcgc gattagccaa agccaatcta cggtttttga tatagtccat tagcgacatc
    70561 cctgcaataa acgtaaagat gcgttttaat tgatattcag agattcctgt attttcggct
    70621 attattttat aggaaagtcc ttctgtcaaa tgttcttcga tgtaatcgat caattggttc
    70681 agatctttaa gcatatttat cttcctcctt gcttgccttt ttatcatatt atcaaagaaa
    70741 gtcaattact tgatgaatca ggaaccaatt tagtcagatg tagaaaatgt tgtatggatc
    70801 taaagaaaat tctcaagcag cgtttcgtaa cggataattg gtattatctg cctatctatg
    70861 ttttaccttt agaaaaataa attttgggga agacacattc taagtaaaca aagcggtaaa
    70921 aatatctttt attcccttag ttaatccgct tactacctac tgtacaaagc aaattgttgt
    70981 tcaaactgct gatcagaacg ctgcttggat ttttctttgc gatacatcat acctctttta
    71041 tcaattggaa caattattta tttctaacaa aatcacttca tttttgtcag ataaataaaa
    71101 aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca cattctctaa
    71161 gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc aaatatgtaa
    71221 aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt gtttcatgaa
    71281 aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg gaacgcggtt
    71341 acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag tacttgtccg
    71401 caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa aagaccaaca
    71461 tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg gctgctgtct
    71521 tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc gaactctcgt
    71581 cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag attcttatag
    71641 aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca ataaattatt
    71701 cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa aacaaaaaca
    71761 ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca gttgaatcgc
    71821 tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg attgctactc
    71881 tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata gaggactgtt
    71941 attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg caaatgttct
    72001 cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc tactccataa
    72061 aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa aaaatagata
    72121 aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa gtaaacgata
    72181 acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca cgaatcatgc
    72241 aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga ctattaggta
    72301 cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat tttccggcat
    72361 tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac tgcatctgta
    72421 tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa tctttaaaga
    72481 taaatattaa aataacgatt aataatatta acagtcttta tttttcacca ccaaaaacct
    72541 atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt gaaaaataat
    72601 gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca aaaaatatga
    72661 tccttattga aattactggc tat
//
LOCUS       DACDEG010000006        66054 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.006, whole genome shotgun sequence.
ACCESSION   DACDEG010000006 DACDEG010000000
VERSION     DACDEG010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 66054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.006"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(65..364)
                     /locus_tag="IV142_000422"
     CDS             complement(65..364)
                     /locus_tag="IV142_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP8484111.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            715..1347
                     /locus_tag="IV142_000423"
     CDS             715..1347
                     /locus_tag="IV142_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP8484112.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            1470..2108
                     /locus_tag="IV142_000424"
     CDS             1470..2108
                     /locus_tag="IV142_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8484113.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(2227..2299)
                     /locus_tag="IV142_000425"
     tRNA            complement(2227..2299)
                     /locus_tag="IV142_000425"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2264..2266),aa:Thr,seq:cgt)
     gene            2420..2662
                     /locus_tag="IV142_000426"
     CDS             2420..2662
                     /locus_tag="IV142_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484114.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            complement(2801..3430)
                     /gene="upp"
                     /locus_tag="IV142_000427"
     CDS             complement(2801..3430)
                     /gene="upp"
                     /locus_tag="IV142_000427"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP8484115.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(3564..4511)
                     /locus_tag="IV142_000428"
     CDS             complement(3564..4511)
                     /locus_tag="IV142_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8484116.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            complement(4664..5908)
                     /locus_tag="IV142_000429"
     CDS             complement(4664..5908)
                     /locus_tag="IV142_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP8484117.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            complement(6083..7108)
                     /locus_tag="IV142_000430"
     CDS             complement(6083..7108)
                     /locus_tag="IV142_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP8484118.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            complement(7130..7309)
                     /locus_tag="IV142_000431"
     CDS             complement(7130..7309)
                     /locus_tag="IV142_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484119.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            complement(7302..8141)
                     /gene="prmC"
                     /locus_tag="IV142_000432"
     CDS             complement(7302..8141)
                     /gene="prmC"
                     /locus_tag="IV142_000432"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP8484120.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            complement(8134..9207)
                     /gene="prfA"
                     /locus_tag="IV142_000433"
     CDS             complement(8134..9207)
                     /gene="prfA"
                     /locus_tag="IV142_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP8484121.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            complement(9210..9794)
                     /locus_tag="IV142_000434"
     CDS             complement(9210..9794)
                     /locus_tag="IV142_000434"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP8484122.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            complement(9900..10369)
                     /locus_tag="IV142_000435"
                     /pseudo
     CDS             complement(9900..10369)
                     /locus_tag="IV142_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            10475..11077
                     /locus_tag="IV142_000436"
     CDS             10475..11077
                     /locus_tag="IV142_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8484123.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            complement(11181..11750)
                     /gene="lepB"
                     /locus_tag="IV142_000437"
     CDS             complement(11181..11750)
                     /gene="lepB"
                     /locus_tag="IV142_000437"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8484124.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(11980..12747)
                     /locus_tag="IV142_000438"
     CDS             complement(11980..12747)
                     /locus_tag="IV142_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484125.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            complement(12744..13223)
                     /locus_tag="IV142_000439"
     CDS             complement(12744..13223)
                     /locus_tag="IV142_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP8484126.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            complement(13348..14085)
                     /locus_tag="IV142_000440"
     CDS             complement(13348..14085)
                     /locus_tag="IV142_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP8484127.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            14246..15646
                     /locus_tag="IV142_000441"
     CDS             14246..15646
                     /locus_tag="IV142_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP8484128.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            15869..17221
                     /locus_tag="IV142_000442"
     CDS             15869..17221
                     /locus_tag="IV142_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP8484129.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            17214..17912
                     /locus_tag="IV142_000443"
     CDS             17214..17912
                     /locus_tag="IV142_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP8484130.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(17974..19125)
                     /locus_tag="IV142_000444"
     CDS             complement(17974..19125)
                     /locus_tag="IV142_000444"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP8484131.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(19138..19575)
                     /locus_tag="IV142_000445"
     CDS             complement(19138..19575)
                     /locus_tag="IV142_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8484132.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(19590..21356)
                     /locus_tag="IV142_000446"
     CDS             complement(19590..21356)
                     /locus_tag="IV142_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP8484133.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            21620..22297
                     /locus_tag="IV142_000447"
     CDS             21620..22297
                     /locus_tag="IV142_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8484134.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            22300..23427
                     /locus_tag="IV142_000448"
     CDS             22300..23427
                     /locus_tag="IV142_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8484135.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(23627..24067)
                     /locus_tag="IV142_000449"
     CDS             complement(23627..24067)
                     /locus_tag="IV142_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8484136.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(24078..26111)
                     /locus_tag="IV142_000450"
     CDS             complement(24078..26111)
                     /locus_tag="IV142_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8484137.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(26113..27816)
                     /locus_tag="IV142_000451"
     CDS             complement(26113..27816)
                     /locus_tag="IV142_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8484138.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(27910..28860)
                     /gene="manA"
                     /locus_tag="IV142_000452"
     CDS             complement(27910..28860)
                     /gene="manA"
                     /locus_tag="IV142_000452"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP8484139.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            complement(28899..29924)
                     /locus_tag="IV142_000453"
     CDS             complement(28899..29924)
                     /locus_tag="IV142_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP8484140.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(30133..31002)
                     /locus_tag="IV142_000454"
     CDS             complement(30133..31002)
                     /locus_tag="IV142_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP8484141.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(31706..33598)
                     /locus_tag="IV142_000455"
     CDS             complement(31706..33598)
                     /locus_tag="IV142_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8484142.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(33595..35322)
                     /locus_tag="IV142_000456"
     CDS             complement(33595..35322)
                     /locus_tag="IV142_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8484143.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(35338..35988)
                     /locus_tag="IV142_000457"
     CDS             complement(35338..35988)
                     /locus_tag="IV142_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8484144.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            36192..36488
                     /locus_tag="IV142_000458"
     CDS             36192..36488
                     /locus_tag="IV142_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8484145.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            36680..37300
                     /locus_tag="IV142_000459"
     CDS             36680..37300
                     /locus_tag="IV142_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP8484146.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(37392..38558)
                     /locus_tag="IV142_000460"
     CDS             complement(37392..38558)
                     /locus_tag="IV142_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8484147.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(38570..38923)
                     /locus_tag="IV142_000461"
     CDS             complement(38570..38923)
                     /locus_tag="IV142_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP8484148.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            39051..40073
                     /locus_tag="IV142_000462"
     CDS             39051..40073
                     /locus_tag="IV142_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP8484149.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(40512..40946)
                     /locus_tag="IV142_000463"
     CDS             complement(40512..40946)
                     /locus_tag="IV142_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484150.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(41056..42915)
                     /locus_tag="IV142_000464"
     CDS             complement(41056..42915)
                     /locus_tag="IV142_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8484151.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDHSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(43033..44193)
                     /locus_tag="IV142_000465"
     CDS             complement(43033..44193)
                     /locus_tag="IV142_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP8484152.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(44500..45924)
                     /gene="araA"
                     /locus_tag="IV142_000466"
     CDS             complement(44500..45924)
                     /gene="araA"
                     /locus_tag="IV142_000466"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP8484153.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(45940..46638)
                     /locus_tag="IV142_000467"
     CDS             complement(45940..46638)
                     /locus_tag="IV142_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP8484154.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(46595..48256)
                     /locus_tag="IV142_000468"
     CDS             complement(46595..48256)
                     /locus_tag="IV142_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP8484155.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            48474..49874
                     /locus_tag="IV142_000469"
     CDS             48474..49874
                     /locus_tag="IV142_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP8484156.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(50126..51205)
                     /locus_tag="IV142_000470"
     CDS             complement(50126..51205)
                     /locus_tag="IV142_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8484157.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(51291..52250)
                     /locus_tag="IV142_000471"
     CDS             complement(51291..52250)
                     /locus_tag="IV142_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP8484158.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(52262..53785)
                     /locus_tag="IV142_000472"
     CDS             complement(52262..53785)
                     /locus_tag="IV142_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP8484159.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(53795..54358)
                     /locus_tag="IV142_000473"
     CDS             complement(53795..54358)
                     /locus_tag="IV142_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP8484160.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(54364..55227)
                     /locus_tag="IV142_000474"
     CDS             complement(54364..55227)
                     /locus_tag="IV142_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8484161.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(55241..56137)
                     /locus_tag="IV142_000475"
     CDS             complement(55241..56137)
                     /locus_tag="IV142_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8484162.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(56312..57583)
                     /locus_tag="IV142_000476"
     CDS             complement(56312..57583)
                     /locus_tag="IV142_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8484163.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(57573..59090)
                     /locus_tag="IV142_000477"
     CDS             complement(57573..59090)
                     /locus_tag="IV142_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP8484164.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            59288..60178
                     /locus_tag="IV142_000478"
     CDS             59288..60178
                     /locus_tag="IV142_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP8484165.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(60251..61075)
                     /gene="nadE"
                     /locus_tag="IV142_000479"
     CDS             complement(60251..61075)
                     /gene="nadE"
                     /locus_tag="IV142_000479"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP8484166.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(61087..62559)
                     /locus_tag="IV142_000480"
     CDS             complement(61087..62559)
                     /locus_tag="IV142_000480"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP8484167.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(63196..63558)
                     /locus_tag="IV142_000481"
     CDS             complement(63196..63558)
                     /locus_tag="IV142_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP8484168.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            63781..64533
                     /locus_tag="IV142_000482"
     CDS             63781..64533
                     /locus_tag="IV142_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8484169.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(64673..65596)
                     /locus_tag="IV142_000483"
     CDS             complement(64673..65596)
                     /locus_tag="IV142_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8484170.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(65611..65883)
                     /locus_tag="IV142_000484"
     CDS             complement(65611..65883)
                     /locus_tag="IV142_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484171.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        19104 a        13534 c        11473 g        21943 t
ORIGIN      
        1 tgaacctagc ctgcattatg agattgacta tacttcttaa ttcattgaga tgaataactc
       61 gtttttataa atgcccaaaa gtaatcggtg ccgaagccat tcctcgacct aattcttttt
      121 caaattttcc tattgttgat atctccatat aaaacggtga tactggttta gacaattctc
      181 ccctgattgc taacggacgt aaactacgtt ccaaattatt tatgacttta ggataatact
      241 gacctttttc taagcctgtc tttgcgtcta tcaaaagtat acgttccttt tctttgatct
      301 ctggatttaa aacgagatca tataatttta gtaccatgtt ttctatttct tcacttttag
      361 acatcacgat ttcctcccaa aaatatattt cttcccacta cattctataa acaaagtata
      421 cgctacatct agattttttt aaatcattac tggattgtta aaacctttca atacactgtt
      481 atggcaacga tttttatttt aaaaacattc taaattaaat cagtaaaaat ttttattcta
      541 agatttttat tagaattacg cctaatttgt gtctctcagg gcaattctaa gaacgtttca
      601 agagatagcg acactatcgt tatcgaaaaa ggcgaaaacc tatctcattt tctgctaatg
      661 taagttggtt tagcttttgt taatatgact ttatgaaaat ggaggtaatt aaatatgatt
      721 tttcctaaag ttaatgaagc aagcttgaat aaaggatcct tttttcatat ctatgaaaac
      781 gaagattttg aactgtataa tatcctttca caaaaagagg tcatcgaaaa ccaaataatc
      841 tgccatccaa ttatttacaa atctattttc aagtatgcat tttttgcttc caatgatttt
      901 caaagaatgg agattaccga ctgtatattc gaaaactgtg atttttcagg ctgtatattg
      961 acaaaaagca tgttgcatcg cgtgaaattt gtcaattgta aattaacagg tactaaattg
     1021 atggaaagtt atattgggaa cacgttattc gagaattgta aaatggacta tgtaaattta
     1081 tctggttcaa acataaaaga aagtaccttt gagcactctg tcttatcaag cgcagatttt
     1141 gtagactgtt ccctcactaa aataatgttt tctactaatg atttagaaaa tgctagcttt
     1201 tttgatactg atttaaaaaa tatcgattta agtagcaact cctttgaaat gattgaagta
     1261 cgctcggaac atctaaaaaa ttgtacgctt aatcagttgc aagcacttgg ttttgctaga
     1321 aaatttttac aaataaaagt agtataatca aaatgaatat ataagataaa tttttagcaa
     1381 tacaaatatc cataaaaaaa cattgcctat tttaactctt catgtatact gactctatct
     1441 tcaaaaactg aatagtagga gttattatca tgaataacat tttaggaaaa aaaatagaac
     1501 ttaaaaaagg gatattgggc actattgtcg ttgtactcgg tttgttaggg ttgctatttg
     1561 cgccatccat tattagcatt ttttctttaa ctatcactgg atttatagca tttacacttg
     1621 tttttggtaa aaaagatacg aaaaaaatgt tctcgaaacc tgtcgctcct gtcaaaaata
     1681 tagccaaata tttttttatc aatgtagtaa tcagtgccgc agtctcggta tttttgcaac
     1741 aaattttgaa atggggttta acaggcaatc caataaatga ggctttcagc ccgttattat
     1801 ttattatttt gcctatcatg atacttggag aagaattgtt ctcagtgtat tttctcgcta
     1861 ttttttctag taagttcagc attcctgtcg ctagtttctt gtcagcaatc atctttggat
     1921 ttatccatta cagtacttat gataatggaa atattttaca cactgtagca cacatccttt
     1981 tgatacaagg ggtggcaaga ttgttattca accaagctgc cattaaaagc aactcgatca
     2041 ttacaagttg ggcagttcat gtgatatttg atttcacagc tatattgcta gtcgttttat
     2101 tctcctaaat ttttatagaa ataatacatg ctctattgct aagaaagcat actttatttc
     2161 ctttaaacaa aaagacagat gtatcaatgg attaaaaatc cttgatatat ctgtcttctc
     2221 ttttaatgcc agctataggg atcgaaccta cgacctacgc gttacgagtg cgttgctcta
     2281 ccaactgagc taagctggcc ttatccaaaa gaaattatat actctatttg gctgattgta
     2341 aagagtccga tggaaaatta gtttaatttc atgatatttt tcataaaacg tggtataatt
     2401 gatgtgaaag gcaggtttta tgaaaatggc aacttttgga aaatttgata caactatcgt
     2461 accatcggaa attggtgaaa aattcaagat caatactcac gtaatgttca caattcacgg
     2521 caagtcgcaa actggaacga tttcaaagca attgaaaaat tccgctgttg ttgagattga
     2581 tgaaactgca gaaaatgcga acttgatgac gcaaagtaag ggtgtagtaa tcgttaacta
     2641 caaagaacta aaaaagattt gaatttgacc atctctgcat ttttgggatg gtttttttat
     2701 atgcaaacga aagctgtgaa aaagctgttc tgcatcaaac gttaaaaatg accaaaaaag
     2761 gatgtgacaa aagttgtgtc acaccctttt tcgtatttac ttattttgta ccaaacaaac
     2821 ggtcacccgc atcccctagt ccaggaacga tataaccatg ttcattcaat ttttcatcta
     2881 gagaagctgt atagatgtca atatcaggat gcgcttcttg caaagctttt actccctcag
     2941 gtgctgctac caaacaaaca aatttaatgt tcgacgcgcc acgttctttc aatgcatcga
     3001 ttgccatgat tgctgatccg cctgttgcca acattggatc tactacaaac aactgacgtg
     3061 catcaatgtc ttctggaagt ttgacgaaat attcatgtgg ttccaatgtt tcttcatctc
     3121 gataaagacc gatatgtccc actttagcag ctggaatcaa ttccaagata ccgtcaacca
     3181 ttccaatacc tgcgcgcaag attggaataa tcgctacttt tttaccagac aacgtttttt
     3241 gtgttgtttc agtgatcggt gtttcgatca cgatgtcttc caatggcata tcccgtgata
     3301 cttcatatgc catcaacata gcgatttcat cgactacttc acgaaaaacc tttgttccgc
     3361 agtttttgtc ccgaataata gttagtttgt gttgaatcaa tggatgatcg atcacttgaa
     3421 atttgcccat tttttaagct ctcctttgtt ctgtcgtttc tttttaattg taaaacaaaa
     3481 tcaagtgatt gactagtcaa actcgccatt tttcctaaga aaatccgttt ttgaaaagaa
     3541 gttttcctgc ggacaagcaa agcttattct tttttcttga tccaagaagt agataatgcc
     3601 attgctgcac gcgttgcctt tgtctgatcc atagcattta ccatgacaaa atcatggatg
     3661 attccttgga agcgtacttg tgtaacagga acccctgcat cacgaagttt gcgcgcataa
     3721 gcctcacctt catctcttaa tacatccgct tctcccgtta aaatcattgc tgaaggaaga
     3781 tcatttagtt catttatatt tgctctcaaa ggcgaagctg tgatttcagc acgctcttct
     3841 tcattcgtcg tatattggtc ccaaaaccac atcattcctt ctcttgtcaa aaaatagttt
     3901 tctccgaatt catgatagga atctgtatca aaatcagcgt ttgtgactgg ataatacagc
     3961 aattgcttgg atattgggag gccctttcgt tcttttgtta agatcgtcat gactgtagcc
     4021 atgttaccac ctactgaatc tcctgcaacg attagattct cgagatccag attttttgac
     4081 ttggctaatt tattcagctg ttgcaacaca gcataatttt gttcaattgc tgttggatat
     4141 tttgcttctg gtgatcgact atattctgga aagacaacga cggaattcgt acgaaccgca
     4201 agttctctga tcaatttatc atgggtttga gcactgccaa aaacccagcc tgctccatga
     4261 atataaaaaa taactggtaa tttatccata ttcccttctg gtcgtacgag acgaatattg
     4321 agttttcccc attcgccagt atccaccatc aagtcttcaa tatcgacgga atatttgttc
     4381 acaggtgatt cttgtacttc ttctagtaaa actcttcctt cttccggtgc tagttcatag
     4441 atacgcggat gaggcgcatt tgcatcactg aatgctactg cttccttttc taacgagata
     4501 cgatcagtca tgacttttcc tccctgagat ttagtgtgaa gactaaaatt acacctcata
     4561 tactagagag catcaccgat attgtcaaag aatccgtttt cccaaaaaat aaacagaaaa
     4621 gcccaacatt gaacttttct gtttactaat ggaatttaaa tttttatttt ttatgcaacg
     4681 gaaatttttc agttaattcg cgtacacctg ttttgacttc gtctagttga gcgttatcat
     4741 cttttgcttg taatgctttg acaactagtt cagccacttt tacagcatct tcttctttga
     4801 acccgcgagt agtgattgct ggcgtgccga ttcgaatacc gcttgttttg aatggactta
     4861 gtgtttcaaa aggaatcgag tttttattta cagtgatatt cacactgtcc aaaatgctct
     4921 ctgcttcttt tccatttata cctaactcgc gaacatcgat caacatcaga tgattatctg
     4981 tcgcccctga aatgactctg gttccaattg cttgattgaa aactttgacc attgcttttg
     5041 cattggcgat gatttgttca ctatattctt taaatgcagg atctaatgct tctttgaatg
     5101 ctactgcttt acccgcaatc acatgctcta gagggccgcc ttgaatacca ggaaataccg
     5161 cactatttat tttttttgct aatgcttcat cgtttgtcaa aatcatgccg cctcgagggc
     5221 cacgcaatgt tttatgagtg gtagtcgttg tgatatctgc ataaggaact ggattaggat
     5281 gaagtcctgc tgctactaat cctgcgatat gtgccatatc taccatcagt tttgcgccta
     5341 cttcatctgc gatttctctg aatttcgcaa agtcgatcgt tcgtccgtaa gcactggcac
     5401 ctgctacgat caattttggt tgatgttttc tagctaaaat acgtaccaca ttataatcaa
     5461 taacttctgt tgtcggatca acaccgtaag caacaaagtg ataggttttg ccactgaagt
     5521 tgactggtga tccatgtgtt agatgtccgc ctgcggaaag atccattcct aagatcgtgt
     5581 cccctggttc taccaaagcg agataagcag ccgtgttggc ttgagagccg gagtgaggct
     5641 ggacgttcgc aaatttcgct ccgaataatt cttttgcacg gtcaatcgct aaattttcaa
     5701 cgatatcgac aaattcacac ccaccataat agcgatgccc tggataacct tctgcgtatt
     5761 tatttgtcaa gatacttcct tgtgcagcca tcactgcttc ggagacaaag ttttcagagg
     5821 caatcagctc caaattatgt tcttgtcttt cttcttcttt ggcgatcgca gcccaaagat
     5881 cagggtcaaa cgttttgtaa tctaccacaa acaacagctc ctttttattt tcctacaatt
     5941 ttatttttcc catcaaaaac aaattgccaa atattcgtat ctcttctatt acgattagtt
     6001 acgcttagtt tttcgttcac tgatagaaga tttacttgat agcattgtat catactaaaa
     6061 gaatgcgaca ataaccaatt gatcattttt caaaataatt ttgtcctgct gcttttttca
     6121 agcgattcat gtaagcattc cccaatccag tttctggata cacttgtaca aaaatcaaat
     6181 ctaatccaag tgttggttca tctaatcctc ttagacctgc aaacaatcct ttagccgccg
     6241 cttctactga gttgtcgtta tacatataaa cagctgcagt atctgtccgc acttgatccg
     6301 caatttcggg gcttgctatg acacctgcac ggatcttctt attttttgcc cattgaactg
     6361 cagttgacca atcgccatct ctgaccatca gaacacgtgt atcgggtgag tagtgtttgt
     6421 atttcatccc aggggctttt ggtgtttcat tttcttttac aagatgctga tcaatagcaa
     6481 caggagattc gatgactgct tcgatttgtt ctttcgttac tgcacctggt cgtaaaatca
     6541 taggcatggc tgttggatca cttaaatcaa gaacagtgga ttctacacca attcgcgtag
     6601 ctccatcatc aataatcccg gtaatttttc cttgtagatc atgatataca tgatctgcag
     6661 tcgttggact tggttttccc gacgtatttg cacttggacc gacaagcgga acaccggaaa
     6721 gttcgattac ttctagtgtt tttttgttat ccggtatacg aaatgcagct gtggataatc
     6781 ctcccgtcac aactgagggc aaagtgtctt ttttgatctt aaatatcaaa gttaatggtc
     6841 caggccagaa gttcttcaca atcttctctg ctgccggatg aaagttgtcc acatactctt
     6901 ttacttgttc aaaagaggcg acatggacga ttaatggatt atcttgcggt ctccctttta
     6961 cagaaaacac tttcttgaca gcgtttggta aaagagcgtt ggcgcccaat ccgtatactg
     7021 tttcggtagg aaaagcaaca agttctcctt ctttcagtgc ttcggctgcc tcttgcaatt
     7081 ctgtttcatc atatcgtttc gtttccacag tttatccaca accccttttt catacattct
     7141 tattttgtct gagtttatcc tcaagttttc cactgtttgt ggataatcta tttgtcaata
     7201 gtggataaac ttgttgataa accaaaaagt cgtttacatc cttgtcaaat aagtgttttt
     7261 ttataataaa aaaacaaata aaaaattttt catccacaac ttcaaccata aacttggatc
     7321 attcgttcat tgccaaaaag atcctttttt atcgccactt ttttatctgg aaatgcttgc
     7381 tgaaatattt tttttactgc ttcaccttgg cgaaatccga tttctaaaaa aatctttcca
     7441 tttgatttta gtacgcttgg tgcttcaact gctatttttt cataaatagc taatccatcg
     7501 ttctcagcaa aaagggccat tttaggttcg aatgaccgga cactttcatc catcagttcc
     7561 cattcattcc gactgatgta cggcggatta gaaataatca catcaaattg ctgatccatt
     7621 actggtccta atgtatctcc atgataaaaa ctgattttcg ttccttcttt ctgagcattt
     7681 tctttcgcta cttctaatgc ttcttctgaa agatccacag cagatatatg ccaatttttt
     7741 cttgctgctt tcaaactaat agcaattgct ccagtccctg tcccaatatc tatcacttct
     7801 aacggtacac caggcgtctc gtctaaacac cattcgacta actcctctgt ttctggtcga
     7861 gggatcagtg ttgcttcagt tacctttaac cgatgatcat aaaaatctgc atatcctagc
     7921 aaatactgag gtggatgatc tgcaagcaga agtcgcagat cggtttcgat caaccgctgt
     7981 tcttcttctg aaatctcttc gttcatatga aggagccaat caagtttttt ccattgtttt
     8041 cgttccaaaa ataaaaattg gatactatat gcttctttcc catgatcctc taaaaaagaa
     8101 gaagcccgtg acaggacttc tcgataggtc attttaccca ttttgcatag cttccagttt
     8161 tgatgtttga tcatacatga tcaatgcatc aacgatctca tctaatttcc ctgctaaaat
     8221 ctgatctaat ttttggatag ttaatccgat acgatgatca gtcacacgat tttgcgggaa
     8281 attatacgtc cggatccgtt ctgaacgatc cccagttccg actgctgatt ttcgattggc
     8341 atcatactcg ctttgtgctt cttgctggat cttatcgtac acacgagcac gcaagatttt
     8401 cattgctttt tcacggttct tgatttgaga acgttcatct tgcatagcaa caacgatccc
     8461 tgttggtaag tgagtcaaac gtacagcaga tgctgtttta ttgacgtgct gtccgccagc
     8521 acctgaagca tgatagatat ctgtacggat atctttgtct tcaatatcga tctctacttc
     8581 ttcagcttct ggcatcacga caactgttgc ggtggaagta tggatacgcc cttgcgattc
     8641 agttgaagga acacgctgga cgcggtgcgc accactttca tattttaatt tcgaatatac
     8701 gttgtctccg gtgatcatca tgatcacttc tttatatcca ccgattccgg taacgttagc
     8761 ttccatcact tctgttttcc agccttgtgc ttcagcatac ttttggtaca tattgaaaag
     8821 atcgccagcg aataatgctg cttcgtctcc gcctgctgct ccacggattt ccatgatgat
     8881 atttttatcg tcatttggat cttttggtaa aagcaagatc ttgatttttt cttctaagac
     8941 ttctttttct ttttttaatt cagataattc ttcttttgct agttcagcca tttcatcatc
     9001 cagtttttca ccaagcagtt cttctgtatc ggtgattcct tgtactactt ctttataacg
     9061 acgataaact tcgactgttt cgcgcgtgtt tgcttcttct tttgaaagct gcataaaacg
     9121 ctttgtatca gaaatcactt ctgggtcact taaaagttct ccaagctctt cataacggtc
     9181 ttcaattgct tgtaattgat cgtacatgct tattctcctt ctgtaatcat tgatgggtga
     9241 aagtaatgtt ttcgacaaac ggggtaataa gcttcatttc cgccaatttg cacttgatcg
     9301 ccttcataaa cagggtttcc atcaatataa tgtaaattca tgattgcttt tttgtggcag
     9361 aaccaacaga ttgttttcat ttcttcgatt ttgtctgcat aaagcaataa atattttgat
     9421 ccttcaaata actcgtttcg aaaatcattt ttcaaaccaa atgccataac cggaatatcc
     9481 aattcgtcca caattctcgt aaaagcaatg acatgtttct tttttaagaa ttgcgcttca
     9541 tcgatcagca cacaataggg tttgctttcc attttctgaa ttacgtcaaa tacgtctgtt
     9601 tcttcaaata tcggcatcgc attacgtttc agtccgattc ggcttgaaac aacgcctaaa
     9661 ccatctcttg tatcaatacc gcttgtcatt aagacaactg gcttattttg ttcttcataa
     9721 ttatgtgcga cttttaggat ctcgatcgtc ttgccgctat tcattgcgcc atacttaaaa
     9781 aatagttgtg ccatgctctt tttcaccttc cagtttgttc ctatggtatt atacttgatt
     9841 ttcagcattt tttacagtga gaattttcat caagcacaga ttagtattaa atctattgct
     9901 cacttttgtt ccaatttttt gacaagaact aaacatgggt tccattcatc ccataaattt
     9961 ggaaaaactt ccaaccttga aaatcctaat gattcgtaga aacaaatagt ttgatcatat
    10021 atagagtagt gcccctcgtc tacagtcttc acttgcaaat atttgtaatg ttttgctgcg
    10081 taaccttctg cttctttaaa aagttttgtt cctaatcctt ggtgatgata gtcttttttt
    10141 attcccatac aatagaatag atttcggcag tatcttcact tgtctgtgaa agagtgataa
    10201 agcctaaata ttcttctttt cttttttcac accataaagg tagtttactt gattcctcga
    10261 tatatttttc cgttgattca ggaagcccaa accactcagg caagtctagt aatatttctc
    10321 ggacacactt tcctttttcc tctctatctt ttacaaacga tatcggcata ttctttctag
    10381 attctccctt tttgaattga gtatagcatg ctttctttct tttcacgtgc tataataagt
    10441 taaaatttca aagttttacg taaggagcta tttgatgaca aaaaatattt ttgattccat
    10501 cgctaaccat tatgattccc ctgaccgtca agcattagcc aagattattc gcgaagaact
    10561 aactacttat ctaccgcgcg acagccacca aaaagtcctg ctggattacg gaggcggaac
    10621 tgggcttgtt agtttaccgc ttgcggaaag attcaaagaa ctgatcattg ccgatgcttc
    10681 agaaactatg ctgaaaatgg cagaagaaaa gatccaagca gcagatctga aaaatgtccg
    10741 aacgatccac gctgatgctt ctgtcgaatt tcctgctgtc caagctaatc tcatccttct
    10801 atcacttgta cttttacaca ttcctgatac ggagaatatt cttacaaaac tttatgaaat
    10861 tttagctcct ggcggtcaat tgatcatcgt agactttgac aagaatgaac aaattagtca
    10921 tccgaaagtc cataatggct ttagccaaga agaattgaat aatcgattgg aaaaaacggg
    10981 ctttgtctct gctactagtc ataccttcca tcgtggggaa aagctattta tgaacaaaaa
    11041 cgcttcgctt ttcttatcca tcagccaaaa agcttaaaat gagattgcaa aaaaagtgag
    11101 caactccaag aaataagaag gaattttcgg aaaaagaagc tgtgacaaaa aacatgtcac
    11161 agcttctttt tattttttca tcacatgatt ttaatttctg aaagaggcca ataacggaaa
    11221 acaacttttc cttggatcat atctttgtcg actaacccaa acattcggct gtcttttgaa
    11281 atcaagcggt tatcgcctag aacaagatac tttccttttg gcacagtata ttcaaagtca
    11341 ctcgtaaagg tggatgaact ttctgcttgt gcttggaatc ctgagctata agcatattct
    11401 ccttgtaact tatcttctga aaattctttt ttgaactcat ccagataagg ttcatcatac
    11461 tttttgccat taatggtcaa ctggtcattt tccatcttga ctgtatctcc aggcataccg
    11521 atgatccgtt taacaatcat ccgttcttca tcccccggtt cctttgtagt aataatatcc
    11581 attcgttcat agtttgagat cttagatgaa atcaatcgtt gtccatcact taatgtaggg
    11641 tccatcgaat gcccgtttac agtaactggt gtaaacacgt acactctagc taataaaata
    11701 gccgctaata caaggatcaa aatcccccaa tttttcaaaa attctttcac agcttacctc
    11761 ctacttgtat tttcctcgtt atttattacg ttttttcaca atacttcgtt gtactttctt
    11821 tattctacaa tgtttataaa tgaaaggaaa gaataagtga tgtgttcgtc ttatctttct
    11881 cgttatataa aaaaggaacg tgacaaaaga cgtgtcacat cccccttatt ccgaataaac
    11941 gtttttatcg caaccactga tacacggcgt ttcatcacat tatgaatgtc ttcttctttg
    12001 gctacgacta taaaccactg gttcttcctg cgactgtgcc ttttttgcaa tcaatttcag
    12061 acgataaggc tcatgatagc gtaacccgtc tttgacaaaa tcaccgccga cgatgaacaa
    12121 tccgacaggt tccaatgtgt cttctgttga gtcaagtcga atggtcccta ctttttccat
    12181 gtccgagtag cttcgtccaa tatttagaac atactctttc tctcctgttt tcttgacata
    12241 gtaataagga tacaatgtat ttctatcgac cagaaatcca tccatgcgac cgagttcatt
    12301 tgttttgatc tcaccatttc caatcaaata ccgcatattc tcggctaatt tcaattgata
    12361 atcttcgtca taagaaacca tgacctcttg tgtattcact ttatcgatcg aatttccatt
    12421 atctatcgat tcttctgtct ggatgttgaa cgcttcatca ggtaaatact tcacaaaaag
    12481 ctgagtgaca tcggctttcg tgtttttatc taaaaagtaa taaaatacat ttaaaacagt
    12541 aaaataaaca agcactagaa atgcaagtcc atataagaaa aattgaagat agttttggtt
    12601 ttgccaaagt ccataaacaa ttctcaataa ataaagcgtg ggaatcacac ttaaaatcgt
    12661 atatccacgg ttaagatatt taggactgat gtctaaatag ccaaggaatt tatgcacccc
    12721 gttgataagg tctagtaaaa atgtcattga gaatcacctg cttgctggtt atacgtttgg
    12781 gtatttcctg aatcttggtt ttgctgtgct gtacgtgctg catcatcatt ttgcgtgtta
    12841 tcatctacag tcgtactgct tggtatcgtt tcggagctag cacttgtatc cggacttgta
    12901 cttggattgg ttgaatccga tggagtgcca tctggtaccg tattacttgt ttcgcttgag
    12961 ctgtcagttg tatatgtctc atcatacgac gaactagttg ttgtatcgtc ccatgtactt
    13021 gtcgtagtgg tttcattcaa actttccgtt gttgttggtt cagatgtcga gactgaagta
    13081 gttggtgttg tttcttctac agtactagaa cttgaacttg gtgcacggta gctggaacta
    13141 ttcacaatac ctgtagtagt cgcgacaatc gtcgagattg ttaatacagc gattggcttc
    13201 aaaataggtg ctacttttcg catcttatct cttccttcat tgaaatttat tcttgtttcc
    13261 ttactttact acggacaaat tatacagtct accttatttc taccataaat tctttatttt
    13321 tttcttacgt ttttccgaaa gatttcttta attttcctca aaaactacgg ttgatgtttt
    13381 taagtaagct tttgcttccc gattgatttc tttcagatcg catccttgct gcttggcagc
    13441 gaacacgcaa ccacaataac attggcgata aatatcatac tctttgcaca tttgcaaaga
    13501 gcgttcatag cctttgtttt tcttgaaatc acttggcaag tattgtgtcc cgtaaatctt
    13561 ttggatatcc attccgatct gattgatcaa agctgcattt ttctttggcg agatggtcag
    13621 ggcacttcca aagtaatcgt atcctagttc ttgcgctttt tgcgcaacaa gatctaatcg
    13681 catattgaag catgcactgc agcgagcacc accttctttt tcgttttcaa gatggtttgt
    13741 taaaaccatt ttggtgaatt catttggacg gtattcatca acgattagcg caacatgatg
    13801 gtcagttttc tcattaaatt ggtctacaaa tcgtttttgt tcatgtaatc ttcgcaaata
    13861 ctcactttta ggatgaatat tcgaatttga aaaaaataac gtgatctctg catattgaga
    13921 catgaattcc aatgtgtgcg tactgcaagg agcacagcat gtatgtatta atatcttagg
    13981 ctttacttct tctttctccc attgcatgat catttttttc agaactgtat cataatttat
    14041 tttttgattt ttcatttttt ctacgatttg ttgagcatta atcaatttgt tattcctcct
    14101 ccttcttttt tctaggattt gccttattat aagccatttc tattttaatc gaaagctgta
    14161 ttgttatctg tttgataatt tctttgagta ttcctaattc cccttgtata ctggtagaaa
    14221 cgactattgt caggaggata gtactgtgca tgttgattta ttctttcgaa ggctgcagcg
    14281 gcgaggacag aagtttgctg ccaatcattt ctcatcgatc caaatcatcg ttttttatta
    14341 tatattgatg acggtcttat cactcgttct attttatatg ccgatttttc gcgaaccaga
    14401 tagccatgtt tcttttgtgg acatgctgtt catggcaatc agtacagtga gtgtgactgg
    14461 actatcgact tttgatatca attctgtgtt caatgataac ggtattatac tgctggaaat
    14521 attatttcaa gttggtggtt tggggatcat gatgatttct actgcttttg tcattttttc
    14581 taaacgacgg atcacactga aacagcgaca gctgatcatg acggatatga atcaaccgcg
    14641 tttgtctggt atcgtccgac tgattcggat cacttttgct attttaatct ggtttcaatt
    14701 actttttggg acattctttt ctatttattt ttattacaga ggctattttg atcgttggcg
    14761 tgatgccgtt ttttacggtt tttatcaggc gatatcggct gtcacgaact ctgggtttga
    14821 cgtcactggg gattccatca agccctttgc acatgactat tttttcttga tcctgatcat
    14881 gtttttgatt tttgtcggag gaattggttt tcctgtattg atggagtgcc gggaatggct
    14941 attatacaaa cgttccaatg cgaagcttcc gtttcgtttt tccttgttta ccaaattagc
    15001 tgtgttagct tttgttatct tgtttgtttc gggaactgtc ttgatttatc tattggaaaa
    15061 ggaccattta ttccaagatt ccaatatgag tgtcaaatgg atcaattcga tgttttattc
    15121 gattacgact cgtaatgctg gtctgcagat tcacgatttg ggtgattttc aaatcacaac
    15181 actgatcatt ttttctttac tgatgtttat cggatgcagt cctagttctg taggcggcgg
    15241 gattcgaaca actactgtag caatcatcgg tttgtatcta tattccttcc tcaaaagtga
    15301 agataacatc aatatttttg gacggagaat cgatcaagat gatgtgcgaa aatcagtcgt
    15361 tgtctttatg ctttctcttg gtatgtgttt cttttgtatc gtttttttat ctgctacaga
    15421 agaacaaacg ctgatctcga tcatcatcga agtaacttct gcgtttggaa caactggttt
    15481 atctttaggg attaccggag acttgtctgt tgtgggcaaa atcacgattg ctacactgat
    15541 gtttattggg cggatcggaa tgctatatac attgatgtta ttcgtgccaa aagaaacacg
    15601 agatctaggc tatgaatatc caagcgagaa aattattatc ggttaaaatg ggaatatagg
    15661 taagagggaa taataaaatc acagcagctt gaaacaatca ttttgtttca agctgttttt
    15721 ttgttaaatc atgcgatttt gtccgatttc atgcactttc agccgtaacc ttttcttaag
    15781 aataagaaaa gcaatttaac ctttttttca acatatggta tgattagttg aatttattcg
    15841 tcagcaaatc gtttttggag gttacataat gggaatacgc agtcattttg cgattgcagt
    15901 tggaaaaacg tcgcaatggg cattaaaaac tttttttaaa ggcggatcaa gtttaccagg
    15961 taaactagcg ctgaaagtcg atccacatat actagatagt ttggcaaaag actaccaagt
    16021 cgtcgttgtc accggaacaa acggtaaaac attgactacc gctttaacag tcaatatttt
    16081 gcgccagcaa ttcgatgaag ttctaaccaa ccctactggt gcgaatatgg tacagggaat
    16141 tgtatcgact ttcttgcagg caaaaccaaa aaaaggacag aaaaagtttg ctgtcctaga
    16201 aatagatgaa gcaagtttga gcaaagtcac agaatatatg aaaccagaac tgttcttatt
    16261 tacaaatatt ttccgtgatc aaatggatcg gtatggagaa atctatacga cctataaaat
    16321 gattgtagac ggtgccgcaa agtcaccaaa tgcaacgatt atcagtaacg gtgattcacc
    16381 aatcttcaat tctatcgaaa cagtcaatcc tagaaaatat tatggctttg accatgaaga
    16441 agatcgtgaa caaatggctc attacaatac agacggtgtc ttatgtccaa attgtcatca
    16501 catcttgcat tataaaatga tcacttatgc aaacttaggg aaatattact gtccaaattg
    16561 tgacttcaaa cgtcctgaat tggattatcg attaactgat atggtacgga tggacaatgt
    16621 gtcagcagat ttcgtgatcg atggtcatga atacggaatc gaagttggcg gtatgtataa
    16681 tgtctacaat gctttagcag ctactgcagt ggcagagtac ttcaatgtcg atcctgataa
    16741 aatcaaacaa ggtttaggat atgacgagaa agtattcggc cgacaagaag tcatcaatat
    16801 caatgggaaa aaatgtacat tagtacttgt caaaaatcct gtaggtatca atcaagtaat
    16861 cgatatgatt ggtctagctc cttatccatt ttctttagtg gctttattaa atgccaatta
    16921 cgcagacgga atcgatgtca gctggatttg ggatggtaat tttgaaaact ttgccgatat
    16981 ggatatccca gctgttattt ctggaggaga ccgtcataca gatatgactt tacgtctaaa
    17041 agttgccggg attccagaaa ataaattgac agaaacaaaa gaattaccag aagtaatcga
    17101 aaaaatcaaa gaattgccaa ctgagcatgt ctatatttta gctacttaca ctgctgtatt
    17161 gcagcttcgt aaagaactag ctgctcaagg atttatcaaa ggaggaatga accgtggcta
    17221 attatgaact caatatcgcc cacctttatg gaaatttgat gaacacatat ggtgataacg
    17281 gaaacctttt gatgttgaaa tatatagcag aaaaaatggg ggtttcttgc catacggaga
    17341 tcgttagtat acatgaacct tttgatgctg ataaatatga cttggttttc tttggcggtg
    17401 gtcaagactt tgagcaatta atcatttcaa aagacatcca gaaaaagaaa gattctttga
    17461 tcaactatat cgaaaatgat ggcgtcatgt tagccatttg tggtggctac caattgctag
    17521 gtcactacta catgggagct aatggagaaa agatccaagg aatcagtgca ctggatcact
    17581 acacgttaag tcaggaaaat aaccggttta tcggtgatat cgttatccat aacgaagaat
    17641 tcaatgaaac ttattatgga tttgaaaacc acaatggtcg gactttctta ggtgaaggtg
    17701 aacgaccttt ggggaaagtg gtacaaggtc aaggaaataa tggtgaagac caatctgaag
    17761 gtgtcattta ccgaaatgtc ttcggttctt atttccatgg acctatcctt gcaagaaatg
    17821 agaacctggc agctcgtttg atccgcctag ctttagaaca acgttacggt aaagaattgg
    17881 atctgcccta tcttgaagga caaacagcct agttattcga aaaaggggaa tgtgacaaaa
    17941 atttcgtcac attccccttt ttcgtatcct attttaagcg acttgcttgg cataacggtt
    18001 cacgttttgc ttgatattcc cgattgtttt cggatcattg attcctgtga cttcttgaat
    18061 caattgttcc aagttttcat ggatcttcat ttcatgcaat tgacggcttt gttcatcttc
    18121 aggatcatag taattaaaaa taatccccat tgttgccgtc aaatgtgggc aagtcaagtt
    18181 atgttcttgt agttctctca ctggacgtgt aaatctttct tgataaccta acttacgtaa
    18241 tggtgtccga gcgacacgtg tgatcgcatc agagatattt ttgttttgga agcgatgaat
    18301 gattttttca atatacgcat gatgctgctc tgcatcaaac ccccatttgg caatcagcaa
    18361 acttcctgtt tcctgcaaaa ctgattttaa ttgtgccaca actaacgcat cttgcattgc
    18421 ttcatcaatg gtctggtaac ctaacaaggc acctgtataa gcgacagtcg catgaccagt
    18481 attgacacta aatagtttgc gttcgatata tggttctaga tcatctacat ataaaacccc
    18541 ttttaattgg atttttttat tttttctttg gctatcgtca atgacccatt ctttgaaagg
    18601 ttccacttgg acgaataaag gatcttcatg gtgttgtaat gggacgattc gatcgactgc
    18661 tgcatttgga aagccgatat atttgtcagt aaatgatgtt tccggaagat atttatacac
    18721 ttctttttct agaaatgctg aaccgccaat catattttca caggcaatga tatctaaaga
    18781 tgttgtgctt ccttcctttt ctctttcttg gatacctttt gcaatcaact cagcaataaa
    18841 aggtaagata ttcgggccaa tggcggtagt aactaaatct gcttcagcga tttcttggat
    18901 tactttttct ggatttgttt gattgttgat tcctttgaca ttttttacag tgattttttc
    18961 ttttgattca tctgctaatt cgatttcgta ttcttttctt tcatttaaag catcaatgat
    19021 cgtgtgattc acatctacga aagtaatctc aaatccatta gctgccaaaa cctctccaat
    19081 aaatcctcga ccgatatttc ctgcaccaaa atgtactgct ctcatttttc tctctcctta
    19141 agcaattgct agattttccg ttacttcttc tttgctgagc gcgtctgcta gctggacaac
    19201 attgtccata tcactgcaat aaagtgcgat ttgctgtaca agttgcaaat gctcatctcc
    19261 gactcctgca ataccgaaca aaacagtcgc aatcttttct tcttcttcgg taccaaaatc
    19321 tacaccatct ggtacttgaa cgacacagat gccggatttt ttcacatatt tttttgcttc
    19381 atctgttcca tgaggaatcg cgataaaatt ccccatataa acagataaca ttttgtctct
    19441 atctaccatg gcatcgatat attcctcatc aacgcaccct gcttctacaa gcatctgacc
    19501 gcaatagcgg atcgcttctt ctttggtttt aaaagatcga ttgagctcga tcatgtcgaa
    19561 tggcaaactt tgcatgctgt tttcctccct taagctgtct gtttcaaata ttgtgcaatc
    19621 gattcgtatt tttgcggatt gaccatgctg ttgactgata aatgaatgga ttgaggtgca
    19681 gccatctgta ctttttccgc taaactttct ttggctacga tcagtgaatc aggttcatct
    19741 tttaaatctt ggatattgac ttttcttaac gggatagtta aatggttctt gtctaatata
    19801 tctttgagca cggtcatccc cattgttgct gatccggaac gatcatcgtg gacaagtaga
    19861 atctctttga tttgtttgat gtttgctgaa agaaactctt ctgctggctc attctcaatt
    19921 tcagaaactg atgttttctg cttgctgctt aattcttgaa tgatttcatc atatcgaggt
    19981 gaattcaaaa agttttctac tgagacaaaa cgcgcgctcg gtgctttttg ttttgctctt
    20041 tcttgtagtt cgacctgagt aacgatcaat gtattcgagt catctgtcag ttggctgatt
    20101 gcttgattgg tcaccgtttg aggcaagtct gcttcctgta ctttttttct caagatcgaa
    20161 gcgcccatcg cacttgatcc cattcctgca tcacaagcaa aaatgatttt ttgtacgcca
    20221 gagaatgtag ctccttgagg aataccttgg gcacctttgg aagcttgctt agctgccttt
    20281 gtctcttgaa cagatttttc gaagtcttca tttgtatctt tcgcatccgc tttcaaaatt
    20341 actgcagaaa taccaaacga aacagctgcg gcaacaatca cgcccaaaat aacaggaagg
    20401 taagaattga acggtgtcat cgctagaatc gcaatgatcg accctggaga agctggcgct
    20461 cttagtccag catccatcaa ttggaaaatg aaacttcctg acattcctcc gcctataact
    20521 gataagaata ataacggttt catcataacg taaggaaaat agatttcatg gatcccgcct
    20581 aaaaagtgaa tgatcatggc tcctggtgct gaagattttg ctgctccttt gccaaacaaa
    20641 gtaaaagcaa gtaatacgcc aagtcctgga ccaggatttg cttcaagcaa gaatagaata
    20701 gattttccag cctctgctac ttgttcagtt cccaatggtg tcaaaatccc atgattgatt
    20761 gcattattca aaaataagat ttttgctggt tcaataaaaa tacttgtcaa tggaatcaaa
    20821 tgggcagata aaatgatctc tacacctttt gccattgctt gagtcaatgc atcgacaact
    20881 ggaccaatcg cagaaaaacc taataacgct aatccaaaac cgataagacc tgctgaaaaa
    20941 ttattgacta gcatttcaaa accggatttg atccgttttt ggaatagctg atcaaatttc
    21001 ttgatggcat atccaccaag aggtcccatg atcatcgcgc ctagcatcat tggaatatct
    21061 gttccgacaa tcacacccat cgttgcaatt gccccaacaa ctgatccgcg gtcaccagca
    21121 attacttttc cgccagtata accgatcaat aaaggaatca gataagttag cattgggctt
    21181 accatggtag cgaaagcctc atttggtaaa taaccatctg ggataaatag agccgttaat
    21241 acaccccagg caatcagtgc tccaatattt ggcatgacca tgcttgataa agtactgcct
    21301 aatttttgta ccctcgcttt tagcgaaatg ttgtttttgg gaatcgtccg ttccattttg
    21361 tctctccctc tctccttttg atactctaat tgtatccgct tactaaaaag agaaatagtc
    21421 gtactttggc acgaaacttt gtgccaaagg tttgagtatg tcagcaaaat aaaaagattt
    21481 cggtatcaga aaacttcaat tttaaataaa actgttattt taattttttt ataatatgca
    21541 aacaataact tttgttagca tttaatttag atgttttcca ttctattatg ttattaaatt
    21601 ttatattgga ggcctatcta tgacattact gatcaaaaac atgaacatga aacgtggtaa
    21661 aaaacgtgtg attcgacaat tgaatctttt agtagaatca ggagaagtag ctgctttgat
    21721 tgccccgaac ggatttggca aaacaacttt gctgaatggc attgcccaac tgctgccaac
    21781 aacttatgat caattgtccc ttcacaacat ctctcctact gcttatacag attatcgccg
    21841 gtctttcttt tttctagaat caacgaatca gttatccgac tatctatcgg ctgttgatta
    21901 tttgactttg atcaaatact attggcagag caaagtagaa tgccaaactg ttttccaacg
    21961 attgaagatg actgagtacc ttactttgcc gattggaaaa atgtctctag gaatgaaaca
    22021 acatgtcttg cttgctgctt acttgatcag tgattcatct gtcatgttat tcgatgaacc
    22081 gttgaacgga ttagacccag gaagtattga tatcttgaat agcctcatca gacaatggca
    22141 gaaagagggg aagacgattc ttctttcttc ccacgctatc gcgaatatcc aatcgatttg
    22201 ttctaaagca tgttttctaa aaaatggaga aatcgtcaca gaatcctcaa atatggaaga
    22261 gattttagct atttatcgta aattatatga ggtgtaacta tgtctttact tacatttgaa
    22321 cgcaatctct tactaaaaaa caaagtaaac tttttatttc ctctattgtt ggtcgtgctc
    22381 tttgcattcc cacttttttt tgatcataaa cttgcttata cagaattcga tgaactgaat
    22441 cataactatg aagagatgca acgtttgatt gaaacgttga aagaagatga gaatgagaaa
    22501 gaatttgtgg aaagtttaga gaaaagcaac aaactgatcg aagcaatctt acatgcaaaa
    22561 aacacaggga atgtccagca aatggtcgaa gcaacttatc attatgaaaa agatatactg
    22621 gatcgactga tttctggtca aagacaagga attccaatca ttgaacaaca aaaacgtgtc
    22681 gagttgctcc gttatatgaa agaacatcag atccaaagat attctatttt tgacttgcca
    22741 gcgcatctct ctttagcaaa ttactacgaa aatatttttt caggaatgat ttctagtttt
    22801 ttgattttat gtataaccgc cttgtttctt tcttccatca tctcttatga aaaaagaaag
    22861 caggtaatct ctctagtcaa tctattaccg gatagtatgg taaagaaaca tagcatccgt
    22921 tttaccattt attatggtgc agctatgcta agtctcgtga tgccattttt gatcgtctct
    22981 atactagtaa taatcaaaaa tggtttggga gacttccgtt atcctgtagg aacgatcatt
    23041 ggtcaggaga tacgaatcct gccgatgtat gagtatcttt tccagagctt tctgttcctg
    23101 ttgttgtggg tgctcttttt aagtacaatc agttttcttc tatctgcttt atttgaacat
    23161 tctctagtca atcttttagg tacacttctt tgtcttttct tagcagagta tcgcttgttt
    23221 tcttcgattg gatggataga atcaatcagt cattacttgc caacttctta tgttgatttt
    23281 caaaatgtga tcataggagg agatattttc tcgccactag cttcagaaca ggttactttc
    23341 atgaatggta tcttgacact tggtatatgg agtatcgttt tattattcat aggtatgggc
    23401 actatctata taaaaaagag ctattaaaac acaaaaataa taggggcggt gacaaaaacc
    23461 ttgtcaccgt cccctatttc aaataaacgg ttttctagaa taaatagttt tcaaaagctt
    23521 gcatccgcag taataagaac ctcccaacaa aaatagctcc tcttattttt atcttggttg
    23581 aagttattac ttgatgcaag gcagcttttt ttactgtgaa cattggttat atatgtccat
    23641 gttcaaaaat catttggact tcttcaatgg tttccgcatc actgagcgcc attacttggt
    23701 cgatgtctga acagaaaaag gctaaatctt ggagcaccgc taactgatgt tcttttttta
    23761 aagccactac aaataagagt gtcgcgattt ttggttcctc ttttgttcca aagtccacac
    23821 catcaggcac ttggactaaa tagattcctt cttctactac ttcttcatct ttgtcagtcc
    23881 catgtggcaa ggctacgaaa ttcccgatat agacactcgc tttttcttgt ctttcgatca
    23941 ttgattggat atatgccggt ttgattcccc cttgttgggt catccactgg ccgatctcgg
    24001 tcaacgcttc tttccaattt tgataggatt gatcgagcaa tattttttct gctggtagct
    24061 gtatcatctt ttcaccttca tttctttatt tcttcaatca ggattttcgc taacaactga
    24121 taaacgattt ctttatttcc tgagcggaag atctcggtgt tcaagtcatt catgatgatt
    24181 gctccactga tctttcctaa aagaatcccg tctttttctt ggattggcga aggcgccagc
    24241 ataactaaca tacgggacaa atcgactggc tgtctatcca tcccttcaat ggtcaacgga
    24301 tgtttcaaat tgaaaataca aaagatcggt ttagaaatca atgagctgga tgtatggaat
    24361 aaccccatat ttgtatgagg aataccgata ggtgcttgtt catatcgctt aagcagccgg
    24421 cgatgaacca cctcttcatc cgttactagt ccttcaggaa aataaggaaa taagctgcga
    24481 atcgtttcgg aaatcgtctc aggattatcc agttcttgga taaaaaaacg accgagtaat
    24541 tgattgattt gatccatgaa atgcattgtt tcctcataag tatcttctat catccactcg
    24601 ttattgctgt tatacgtaat tttttgcctt tttttcttct caatatgcga aaaagcttct
    24661 tttagctgct tgacttcatc ttctagcagt agcggggaga ctaaaatata ttttccaccg
    24721 tatcctggta aaatagatgt agaaataaca acatcatatt cttcttgtaa atcgattttt
    24781 tttaaatcag caatccgata gaaatgaatt tgttggatga agggaaaata ccttttcaat
    24841 cgtagttcca acatgctagt agaagctaat ccacttggcg aaaatcctgc aatactgata
    24901 ttgctttctt tcggacttcg ttccaatgaa ttcgcaaaat gaaggaccat ataagcgatc
    24961 tcttcttcgg ataatttttt ttcttgaaaa atccgctcta ctgctttacg gattgcattc
    25021 gcaacttcct cgtattgaac catgattttt tctaaaatcg gattggtcag tgtctcttct
    25081 tgtaaaacgg ctcttgaaaa cacaccagat aaatgagtca acagcatttt gtacaaagtt
    25141 tcatcttcat aaaaatggac attcgtctct tcacttactt cttgaatcag ttgttttact
    25201 aaataagcaa gctgtgtatc gaacgtctct tcaaagaaat tctgatcaaa tgagttcgtg
    25261 aagtcattta ataatcctgc aaaaaagacg atctcattta ctggataaag ttgttttgtc
    25321 tcagaagcga tccgtgaaaa caatcgttta gctgtctgta gtaattcttt gtttgccaga
    25381 ctattatatg tctcatttgt cagtaaatga tttttcgtca cacgctccat cgtcaatgtc
    25441 agcatcaaaa ccaaatgttc aagctttcta tcacttagat tggtcttttt ttggatcaat
    25501 tcttccccga ttactttgcg tgcaagagat agatgttgcg gatcaacgaa tcgaaaaaaa
    25561 taattcagtg atgcatcgaa agaaatacta tgaaaaagtt catattcatt gatttctaat
    25621 tctaatacat tcgctgtcag aagccggcgg tatttttccg gtccttcgat ctcgtatccc
    25681 tggttccgaa tgatttccaa aggcagttgg cgaatacttt cttccaactg tttgatatct
    25741 gcataaaaag tggtattact gatgcgatat gattccatga aatgagcaag actcaacggc
    25801 tctgtcgtca gtaacaactc ggctaaaatc gcgtgttgtc gtttagcagt ggataagtca
    25861 ttacttttcc catctgtatt ttcttttaat tgctctaata cttcttctga tgcctcaatg
    25921 acaaatgagc ctctagctgc ttttttcaaa gaagcattta ccgatttcaa tgagacttca
    25981 agattatcta attcgcggta aatagtccgt ttactgacct tcaataaatt catcattttt
    26041 tggatagata caggtgacgg gctactgaga aattctgcta ataattgttt ttctctgggc
    26101 gataaataca tcttatattt ctcccttcac gagtttttcg tattcattcg tagctaccca
    26161 atgttcaact agaactagcc gagggatctt ttcttctagc tgttcagtta cagctagttc
    26221 tctttgtgcg atataaatcg tttcttcttg gattttgatt ttttctattg attcttttga
    26281 gataacaaac tgttttttcg ctttttttgc taattgactt aaaatcgtca tgcccatagt
    26341 ttgtgagccg cgtctgttgt ttttatacaa aaatacaata ttagcaggcg acgttttttg
    26401 ttctgtgata tctgctatct cttttttttc ttcccgcttt ggaaattgga tcgcttcttt
    26461 tattcgtgac agcaagatat ctgtttccaa aaagtgatcc agtacataga tttgactttg
    26521 aggtactttt tctcttgcaa gacccgctag ttcatgttgc acgataacca gctgatttac
    26581 ttggttattc aattcataga tagagctgta tccgacagaa agatcgattt tttctgctgc
    26641 taatttcttt ttcagcaggg cggctcccat agcacttgat cccatacctg agtcgcaagc
    26701 aaaaatgacg gaatgaatga tagatggttc caagatttct cccctttctt tgaccgtttt
    26761 gttctctttt tctttatttg gtaatttcaa tagtatcgct gcacaaatga aggtcacaaa
    26821 tgtactgatt gctatcccta tacttacccc agctagttgt ggataaggcg cattggcaaa
    26881 aatcacgagt agtgacccag gcgatacagg tgcagctaat cccgtatcta accattgaaa
    26941 tatcaacgtg ccgcttgccc caccagccat tacagctaaa aatagatacg gtcgcagcaa
    27001 gacaaatgga aaatagattt cgtgaatccc gccaatcgca tggatcatca atgctccact
    27061 cgcatttact ttttcctgtt tttctgcaaa acaaaggata gaaagtaaga tccctaaacc
    27121 aggtccagga tttgcctcca tcaaaaataa ggaagagtac ccgactgttt gtgagaattc
    27181 gattcctaaa ggtgtcaata caccatgatt gattgcatta ttgaaaaata gtacttttag
    27241 aggttctaag atcagactga caagtggtaa cagattcatt gtgactagcc aagacacaac
    27301 ggaggcgagc aacaagtgaa cactatttaa aactggttca ataaaaacga ttccaattat
    27361 acaaataacg ctaccagtca gtcctactag taggttgctg acaagcattt catatccagc
    27421 cttgaatttc acatggaatc tagaaacaaa ttgttcatat acgatgccgg caagcggccc
    27481 gataatcatc gctcctacga tttgtggagc attggaacga gcgatcaacc ctaaagaagc
    27541 aattgcaccg gcgactgccc ctttttgtcc ccctaccgat ttcccgcctg aataactgat
    27601 caagatgggt aaaagcagct ggatcatcca atggcatacc tcttctaatg tggtgtgcaa
    27661 atcgccagac acaccttttg ttgctaatga caagattccc caagcgataa acagacttag
    27721 atttggcatg atcatttcat ttaagtgatg tccagctttt ctcagttgtt tcatagacac
    27781 gcgactgctg ttgtttgctt tccatttgta ttccatcttt ttcaccagac ttctatttat
    27841 tgatggacgg attttatgta gaaaaagcag caaacgaaaa accgtctgct gctttcagct
    27901 atctgggaac tatttgcctg gttcagacgc aatgattgct agttcacctt ggaccgccca
    27961 ctcagaaacg ccatttggaa taatacagct tgttcctttt tctaatggat atgtttttcc
    28021 gtcaacgaca aggtctcctg ctccttcgat taccgtcatc aatgtataag gggcttgttt
    28081 tttgaaagat gtgattcctt taatatccca ctcatataca ttgaaaaact cagtttctaa
    28141 ataagtgacg atcgatgaat tcccttttcg gacttctttg atttgtaatt ctggtgtttt
    28201 cgcagggaca gttgttacat cgatcgattg ttggatatgc agctctcttg tttgtccttg
    28261 cgcatctttt ctgtcgtagt catacacgcg gtaagtcgtg tcagaacttt gctgtgtttc
    28321 taaaatcatg attcctttac caatagcatg gatcgttccg cttggtacat aaaagaaatc
    28381 acctttttta actggtactt tcttcaaaag gtcatcccag cgcccatctt tgatcatctc
    28441 agctagttct tctcttgtct ttgcatgatg accatagatg atttctgcac caggttcagc
    28501 atcgatgata taccagcatt cagtttttcc taattcacct tcatgtttca tcccataggc
    28561 atcatctgga tgtacttgta cagatagatc atcttctgcg tctaagatct tgatcaataa
    28621 tggaaacact ggttctgaag gatggccaaa caattccttg tgttctgacc atagttcatc
    28681 taatcttttc cctttgaatt cgccattttc tactacactg acaccatgag gatgcgcact
    28741 gatcgcccaa tcttctccga ttttatcgtt agggatatca aaaccaaaga ctgaacgtaa
    28801 acgacttcct ccccaaattt tttcttgaaa aactggtttt aaaaacatag gttcctgcat
    28861 aattcaaact cctctcattt ttcatagcta ttatagcatt atttcggtaa taccgctttc
    28921 tctttcaaaa agttttcaac aatttcatct cgacatatga gatactccgc acgatgatcg
    28981 acaggatgta cgcgatttga caaaaatatc aacgcttctt gtgtttttat atcaatcaag
    29041 aaaaacgtac ctgtataacc agtatgaaaa agaatcgcat gaccgtcttc tacatccttt
    29101 tttaagtccc atcctaaaga acgatagcca ttttttttcg gtgtttgatc ttgcagtaaa
    29161 gaatcaatcg tctcttcttc taaaaaacga ccattacgga tttttccctt gttcaaatac
    29221 atttggacaa aagtaatgct gtctttcaaa tcaataaaga gacccgcgtt acctgcatgt
    29281 tcagccaaaa ctcttgcttt cggatcatga gtttgtccac gcaaaacctc acccgaaggc
    29341 agttgttctg tgggaacgat ttttttggaa ggaaaaggaa gaaataagct gttcatcatt
    29401 cctaatggtt ccaatacttg ttcttgaaag atttccactg cagtttcttg gtaaatttcc
    29461 tctagcatca acccaagaag aatcgtaccg gcatctgtat attttacttc ttttcctagt
    29521 tgctcacctg gttctagctt caaataagcg gcgatcaatt cttcctgaga tagcttatct
    29581 ctgtttggta tccatgtttt gatatcagaa gtatgtgtca gtaaatgacg caaagtgatt
    29641 gtctgatttt caaattcagg aagatattta tgaagcggtt gatcgagttc tatctttcca
    29701 tcttctaata gacgtaaaac taatgaagtg gttgcaacga ctttcgtcag tgaggctgca
    29761 tcaaacaaca gatcttctgt catcagctgc ttttcaggaa cgacttgggc aaagccggct
    29821 gtaaatgttt ctacttgatt ttcttttatg aaggcgactg ctacacctgg ataaaatcct
    29881 tcttccatct ttttcaaaat aagctgtttc gtgtgtggat acatcttttt ctcctgcttt
    29941 tctatcagat tcttacagtc caagcttaaa gcttaatcta tgaggaatca acctttttta
    30001 cacaaaaaaa ctgcgcgaaa aaccgcgcag tttcccccgg tatactattt tctctttcta
    30061 tctctagaat aagcgatagt atgaccgttc ataaaatgac gagacatgcg acaatcgtta
    30121 agaaagaaaa agttaagcgt cgcatcgaat gtcagtttta ttcttttctt ttggctcact
    30181 gacgaaccac cattcgattc ctatagcatc atagattgtg agtactgtgt gctttttgtc
    30241 taagaagaaa tcaactgata gttgctgcaa gtgagcaatc agtccttcca tgctttcagt
    30301 atcgatcatg aatttcagaa aatcaagtcc aatcacttca tctgtaggca aatcaattgc
    30361 gcctccgcta gcttctgtca aaccaacatg gaaatctcct tcattcaata catggatgcc
    30421 ctcttcttca ttatgtacct tcaaaccaag tacatgatta agaaattggc gttcttttgt
    30481 taagttagta acatttaaat ggattttttc aaaataagaa cctgttgata gtttactgaa
    30541 tttttcttca gattttctta ataatgcttc ttgatccatt gtttctggaa tttctctttg
    30601 gtcgttctta ttctcttcat ggtaaatttc caattcattc tcttctgggt ctacgagcaa
    30661 aatccccata cgtccatgat cttctaaagc ttctttgatt ggataattca ttttacggat
    30721 acgacaaagg atatctgcca tttcttctgt gctagggata atcaatgaaa aacggttcag
    30781 ctttttgatc acccccatgt gatcattcgc tctaggactt tcttccagca gcaacatttc
    30841 acttccagct tcttctgttc ctaaaatggc taattcgttt tcttctcttt tcaaatcaaa
    30901 gccaacaacg ttcttgtaaa aatcaatcat tttatctcgg tctttcaccc gaatggctac
    30961 actttttaaa taggtggcag cccccaattg aaaatctttc atatttcttg ctcccctcta
    31021 cgtcaagctt tattttaacg atggattctt cttctttcaa gcaataactt tgattttgtt
    31081 aaaaaaaatg tgagaaaatt cgcattttgc ttgttttttg taaattaatg agtaaaaact
    31141 cttataaaaa gaggttgtga aaaagctgtt ctgcatcaag taataagaac agccaaacaa
    31201 aaatagctcc tcctattttt cgtttggttg ggcttattac cgcagatgca agcttttgaa
    31261 caccgtttaa ggaagaggtt gtgaaaaagt cgcttagttt cgagtagtaa gaaaaatttt
    31321 gtgaaaatgg ctttctacat ttttgcataa atttggctta ataccgagaa actggctttt
    31381 gaacaccgtt taccaagagg ttgtgagcct gccgtttaac tccgagtcac aaggagcaat
    31441 ttcaaaaaac agcttctcag gtttgttgac tattgtgact tgtgaccgag gagttggcag
    31501 gagaacaccg tttaaggaag aggttgtaaa atcagcggtt tgacctgagt attagagata
    31561 caaaacggaa aaatagcttc tcatattttg ccgttttttg agctaatacc gaaggacagc
    31621 tgattgaaca ccgtttattc ggaaaaaggg actgtgacac gacttttgtc acagccccta
    31681 aaaaaaataa taaatttgta ctattctatg aagcttcttc ttcagaaaac tgactgttat
    31741 aaagatctgc atagaatccg tctttttcca tcaatgtatc gtggtttcct gtttctacga
    31801 tcgaaccatg attcatcaca ataatgttgt ctgcatcgcg tattgtagac aagcggtgag
    31861 caacaacgaa acttgtccgg ttttccaata ggcggttcat cgctttttga atcagtattt
    31921 ctgttcgtgt atctacacta gatgttgctt catcaaggat caggacatct ggatttgcta
    31981 agaatgcccg cgcaattgtg attagctgac gctgcccttg cgaaatattg cttgcatcct
    32041 cgtttaatac agtgttatat ccttctggca atttacgaac aaaatcgtct acgtgagcag
    32101 ctttagcagc ctcaatgatt tgttcgtctg ttgcatcgtc gttcccatat ttgatattgt
    32161 catagatact tcctgtaaac agccaagtat cttgcagtac cattgaaaac tgtgcgcgca
    32221 attcatcacg tgaaagatca cgggtatcta caccatcgta acgaatacta cctccagaaa
    32281 catcgtagaa ccgttcaaga aggttgatca gtgtcgtctt ccctgcacca gtcggaccta
    32341 caatagcgac catctcacca ggtttgacat ccaagttaaa atcagtcatt agcaagttat
    32401 ctggtgtgta gccgaattga acattttcaa aacgaacttt gtatggtgta tctttttctt
    32461 ctggaaggcc agatttttca ttggacatct cttcttcatc caacacttca aatacccgtt
    32521 cggctgaagc aactgttgat tggatagtgt tcatcaaact tgcaagttga gtgattggtt
    32581 gagagaactg tgttgtatat tgcaagaacg cttgaacatc ccctaatgtc atgttcccat
    32641 ttgcaacttt tactccgcct agtacagcga cgaacacata gcctagattt ttaacgaaat
    32701 tcatcaacgg catgatgatt gccgaaataa attgagcttt ccaaccagcg gcatacagat
    32761 tctgattttc tttttcaaat acttcctggt ctttttcagt atgattgaat gttttaacaa
    32821 tggtatgacc accatatgtt tcttctactt ggttattcaa taaacctaaa cttttttgct
    32881 gatctgcgaa aaacacttgt gatttcggcg ctacgatcat cacgatgatc aagcttaaag
    32941 ggatcatcaa taacgcgact aacgtcaatt gccagctgat tgaaagcatc atcacgatga
    33001 ccccaatcaa tgtgactgca ctagttacaa attgagtcaa attttgctgc agcgtgcttg
    33061 cgatgttatc catatcattg attgctcgag acatgatatc cccatttgta tgtgtatcat
    33121 aataggaaat cggtacacga ttcattttgg cttccaaatc atgacgtaat tcataaactg
    33181 ttcgttggga aacacgtgtc ataataaatt gctgtaaaaa gttgaataca gcagaaatca
    33241 aatacatcac gattactgtc gcaatgatct gcgcgatttt atcaaagtct atcggaaatt
    33301 ttgttacttg ttggccttgt tgcatctgtt gagcgccagc cattacacct ttgaatattt
    33361 ctgtggttgc tttccccaag acctttggtg tttggatttg gaagatcacg gctgctattg
    33421 ctagtacaaa tacagcgatg atcgcaacca tacgtttaga catatagcca aacaatcggc
    33481 gtaccgttcc ccagaaattt ttaggctttt caccttttac accgagattt ctgccaggtc
    33541 caccatgtcc tggaccgcct gctggtccat gagagggacg tgtgtttgtt ttactcatgc
    33601 taagtcctcc tctcttagtt gtgaatgaac gatttcttga taagtttcgt ttgttgctaa
    33661 tagttcttca tgtgttcctt tgccaaccag ttttccttca tctagcacaa ggatcatatc
    33721 tgcatccatt actgtactta ctcgttgagc aactaaaacg gtgatacttt ctttcatatt
    33781 cgttttcaat gcttggcgaa gattggcatc cgttttgaaa tccaacgctg agaaggaatc
    33841 atcaaacaca tagatatctg ctgttttgac taatgcccgt gcaattgcca gacgctgtct
    33901 ttgtccacca gagaagtttc ctcctccttg ttccacatgg ctatctaagc catcttccat
    33961 ctctgaaaca aaatctttag cttgtgcgat ttctaatgct ttccaaatca tttcatctgt
    34021 tgcatccggc ttaccatact gcatattgtc tcggatcgtt ccagagaaca gaactgcttt
    34081 ttgaggaaca aagcccatta attctcgcaa attatattga gtcatgtcac taatgtccgt
    34141 tccatttatt tggatcgaac cagattcgat gtcatataat cgagggatca aattgactaa
    34201 ggtcgtttta cctgaaccag tcccccctat gattgcgacg atctcgcccg acttggcttg
    34261 gaaatcaata tcttccaatg caagtttttc tgctccatga tatcgataat tgacatgatt
    34321 gaaggccaat gttgcgtttt ttccagcaaa agaaactgtt ttaggatttt cagggtcatg
    34381 gataccgatt ttttcatcca acacttcatt aatacggtca gctgaagctt gtgcacgagg
    34441 caccatgata aaaatagcgg aaagcatcat aaagctgatc aagatctgca tcgcataagt
    34501 cataaatgca atcagattac cgacttccaa agtcatatct gctatgtagt gtccaccaaa
    34561 ccaagtgatt gctacgtttg taccgctcat gatcaatgtc atcattggca tcatcaatgc
    34621 gacgatcgta ttaactttga ttgccgtatt tgtataatct ttattcgcta aatcaaaacg
    34681 attttcttca aagcgtgtct tatcgaatgc tcgaatcact ctgacccctg tcaatccttc
    34741 tcgaaatacc aggtttaaac ggtcagtttt cttttgcata cttttgaata atggaacagc
    34801 aaagtacatg attccgccga tcaaaataat cattactggg atgacaacta agaagatttt
    34861 tgtcaactga tgatcttttt gataagcaag tatgctcgcg ccaatcaacg tgattggtgc
    34921 attgatcatc atacgtaaaa acatttgtgt aaccatttgg atttgagtca catcattggt
    34981 cgttcttgtg atcaaagagg ccgttccata tttatcaaac gcatcttttg tcagactctc
    35041 tgatttttta tagacatccg tacgcaattg cttacccagt ttctgtgatt cacgagtagc
    35101 aaaaaatgta tttgcgatag cagctaagat actgatcaaa gaaaagccga tcatcacaaa
    35161 acctgtatgc caaatataat cgacatctcc ttgcgctacc cctttatcaa taatattgga
    35221 ggtaagtgtc ggcaaatata aatctgccag tacttggatc accatgaaaa ctgcagctgc
    35281 aattgctgaa gtcaaggaga tccgttttac gatttttagc atctagttcc cttctttcta
    35341 tttatatact ccgtttttca gccaagaaag tttcaaattg aattcactga ttgcttgctc
    35401 tactgagtaa tcttcagatg ttgaaaataa gcgatacgaa tgcgcaattg ttgtaaatac
    35461 agtattcatc actaattgga tcagtaattc caattcatga tcatttttta ctttcagctt
    35521 cgtccgatcg atcaattcaa gcaactcttt tgcatcttgt ttgtgttctt tatgtggatc
    35581 gtgaggcgtt ttttggcgac tcgtatgagg agcgactttt cttgaggcac ggtaatccat
    35641 gttcataaac aagttacgat aaaaagaagc attctcgcct tccaaaatct cataaatcat
    35701 tttagaaaaa taggattcga atcctttgaa tagatcacca tcggattctt tcattagttg
    35761 taacatatct ttagagctgt cacgtctcat tgtctcaaag taataaaaat aaagatcctc
    35821 tttatcttca aaatactgat agaaactgcc tctagggata tccgctaatt tgacgatatt
    35881 agcaatcgaa gcttctttta agggtactct agaaaactcg attctcgcag cctcgattag
    35941 tcttttttgt ttttctttag gcaaatggaa aaatgtctgt gtcggcatat ggattctcct
    36001 ttctttcaag atgacacatt gtcatatgac gctatgtcat tataacacct cctacctgtt
    36061 ttgcaagaca tttgcatagt ttttttcatt atcatccgat acattaaaaa agtgataaga
    36121 ggaatccttc catttgaatt aatgagagtt gcccattaaa atagccacat actaaacaaa
    36181 ggatgagata aatggcccaa tcaattagtg tccaagactt caataaattg cctctcgatg
    36241 ctgacagcat cgttttagat acaagaaata ccttagatta cacaaaaaac cacttaagcc
    36301 actcgctttc tatccccgct gctatcttgc caagacaatt aaaaaatcta gatccaaaga
    36361 aactttatta tattctaagc tatacaggca ggcgatcaga agcattagcc gatcaactta
    36421 ctgctaatgg ttttcgtgct gtttatatca ttggtgggat ggaagctctt cagtcgagtg
    36481 ttgcttgaat attgcttgtt agattcaaaa gaggctgtga caaaaaatcg tgtcacagcc
    36541 tcttttatac cctgtctttt tttagttatt tattcttcaa ccttatcaat atgacttgat
    36601 agccttttct ttttcgagta aactgatttt gttagtcata gttataacaa tcatagcaat
    36661 aaaaggagaa ggtaagcata tggcatctgt tttagtagtc aaaggtcatc ccctaactgc
    36721 agaagaatcg cgcacagtta aggcattgac gtctttttta acaagttata aagaaaatca
    36781 tccagatgat gaagtgactg ttttggaact ttatcgcgat gatattccgg aaatcgatga
    36841 agaattatta tcaggttggg aagcattacg tgctggtgca gaattcactt ccttatcaga
    36901 aagtcaacaa caaaaaattg ctcgtttcaa tgaattgact gatcagttcc ttgctgctga
    36961 taaaatcgtt atcgcaaacg ccttatggaa tctaaatatc ccaacaaaat taaaagcttg
    37021 gttcgatacc gtcaatgtag ctggaaaaac gttccgctat acagaaaacg ggccagaagg
    37081 attagtgaca ggtaaaaaag cactgcacat ccaatctaat ggcggtgtat acaacggaca
    37141 agactttgcc tctcaatatg taaaaggtat tttaaacttt gtaggcatcg atcaagtaga
    37201 tcagctattc atcgaaggaa tcgattatga ccctgaccgt gcagatgagt tgatgcaaaa
    37261 tgcattagat aaagctgctg ctttaggtaa atcattttaa aaattgagat aaatgcatac
    37321 aaaagagaca gaggaaaaaa tcttcaaagc agcatttgtg ccttggatat tttcccatct
    37381 gtctcttttt gttattcttt ctgctttttc aacggatttt cttcataaag agaagagatt
    37441 gttgtgtacc attcaaaaac atgagtaatc actactttag ctgctgcata aacaggaatc
    37501 cccaagatca caccgatgat tccgaacaat ttcccagaag tcagcagaac taataaaatc
    37561 gttactggat ggattgctaa ctgattccct agtactaatg gagagatcaa gcgtccttct
    37621 attgtctgct ctactgcaaa aacgatcagt actttgatga tcatgaatgg acctgctaca
    37681 atggctaaaa agatagccgg aatcatcgct aaaaatgatc ctaagtaagg gatcaggttt
    37741 aaaaaacccg caataatccc caacgtgaca gcatactcta aaccaatgac cgcaaaacca
    37801 atcataaaca tgattgccac agcaaaagcc acagttagtt gtcctcttat ataagaagaa
    37861 acttgactat tcatttcact cagcacattc aacgtcggtt tttgcatgcg tgtcggtaaa
    37921 aatttgacaa aataaggtgc taattgttta ccgtctttca atagataaaa taagataaac
    37981 ggcatcgtca gcagagccac taaaatagtc gctaccgcgc cgacaaaact tccaagactc
    38041 tgaactgtac tcttagatat atcttggatc atatcgcctg ccgaattcat gaacttatca
    38101 cctgctgttt ccaactgttc gcgaaattgg ttgaacagcg ggtcccgcaa aatctcggtt
    38161 aatttattat caatcgtatc tacatattga gggaagttac tgatgaagct cactgtttgt
    38221 tcctgaatct tagggatgat cactacgctc ccccatgcga ttagtgcaac aaccaaaaca
    38281 aacagcccaa tgatactgta aagccttggt acttttcgct tttcaaagta atcaactacc
    38341 ggattcatca agtaatacaa gattcctgct aaaatgatag gcagacctac gattgctaaa
    38401 aattgccaga ttggtgcaaa caaatatgaa acttttgtaa ataaaaagac gatcagcaat
    38461 attaacaata caacaagtaa ggctgtgact acttgattat ttaaaaacca gcgccaaaac
    38521 cacgataccg gtctgttctt cttatttttt ccttccataa ttcctcctgt tagttataaa
    38581 aaatttggct tcccaaacca atagcaggca gtctatgcgg atgaagatag attccatttg
    38641 caatttgtcc ttcttctgga caagcatcaa aaatcaatga tacgtgagct actgtcttta
    38701 aattcccatt ggccatcgga ccgacataat cgatcgtata tacctgatca tcaaaagcaa
    38761 ttgttccgcc tgctttcaaa gaaaaaggtt cgtcttttga tacttcttga atcaccgaat
    38821 attttcgtaa ggcagctgtt gctgttttat cgaacaaaat cagcatgggt tcttccttgt
    38881 ttattgcttc tgtcccaatt tctgtaatct ttgcctgaat cattgcactc tctcctttaa
    38941 ttgctttcaa aaacagtata acacattctc attctttagc cgtgtggaat aattatgtga
    39001 tagaataaaa acgaaaacta tttcattaca attaggagga atcagctcat atgatcgaaa
    39061 agataatttt aggaacctat acgcgtcgcg aaagtaaagg aatctatacg atcgaccttg
    39121 atacagaaaa agaagaatta gccaaccttt cattagctgt tgaagaaaat agccctacct
    39181 atgtagcaaa aagtaaagct ggcaatcttt acacagttac tagtgtggat ggattaggtg
    39241 gtgccggtgc gtacgataag gattttcatt ttttgaatgc ggtaacagag tctggcgctc
    39301 ctttgtgtta tgtagcggtt gatgaagcac gtcaattact atacggggcg aactatcata
    39361 aaggtgaagt aaatgtctac catatattag gagatggcga actgaaagca gcagattcaa
    39421 tattccatca agaagcgaca ggtcctcata aaaaccaaga tcatgcgcat gtccactata
    39481 cggatctgac tcctgaccaa cgattagtcg tatgtgatct tggtacagat cgtgtgtata
    39541 cttatgatgt gtccgaaaac ggcaaattga atcttgtaac taccttcact gcagagccag
    39601 gaacaggccc tcgccatctt gtctttcatc caaatggaca attcgcttat ttatttggtg
    39661 aattagacag cacagtccgc gtgctttctt ataataaaga aacaggtgct tttgaagaat
    39721 tacagaaaat atcgaccttg ccagaagaat tcgacggtga aaatggaggg gcagctatcc
    39781 gcatttcaaa tgatggcaag ttcctttatg cttctaaccg aggacataac tcgattgctg
    39841 tattcgcagt ttttgaagat ggttcccaca ttcaaaacaa gcaaatcatc tcaacagaag
    39901 gggattttcc ccgcgatttc gcactagatc caagcaataa ttttgtcgtg tgtgccaatc
    39961 agaacaccga taatctgaca ttatatcgcc gtcatcctga aacaggatta ctggaattac
    40021 tgaaaaaaga tgtctatgta ccagaatgtg tctgtgttta ttttgatcgt tagtaagaaa
    40081 aggagaatga aatgaatcag caagacgtat tagacagact acgggaagaa ctaaatattc
    40141 ctttctttga tgggaaaatc gaagataaag agtatagcga agaggaatat cagaaactca
    40201 aagccgattt acagaactac tttgaacaat atgtacagaa tgtagaaaac taatcagctt
    40261 tccatactat ctgaaaagaa catcaggaca agacttttcc gtctatcctg atgttctttt
    40321 ctacaaataa aaaaccgccg agatggttag actcgacgga aaaggagtat ttactcgatt
    40381 ttgagtaagt acaaggttgt tgttggttat aggtatgtca ttatcataaa ggattttgat
    40441 cactctatca taaaggtaat attacattta tattagataa ctctagtttt tattgttatc
    40501 aaagatttct tttatttttt caacaacaaa ttagtcattt tttggatggc agctgtcaat
    40561 tggtctgtcg cttggtcttg ttttgcttca gatgtattga atagatcttc atctacccct
    40621 gattcaagat cgatactttg gatcatttct tcacagcctg ctacatatga aagataagct
    40681 ttttcaaaat ttttatgaat gcccattgct tttgctggtg gacgcaaagt actgactttc
    40741 ataagcattg cccgatattt ttttgttcct tcttcaaata acaggatgat ctcttcaaga
    40801 gtcgatttgt ccagttcact tgtttttcct tcatcaattg cgtttctgat cacttcatat
    40861 ttcggatgca ttccttctcc gattgcttct gtttccttga cgatttgatc gatggactgc
    40921 acataatagc caatattcgg taacatgttc gttttcctac tttctccatt gttgttatta
    40981 tttattctac ttgtttttat cctcaaatga caagtacttg aaaaaattta atggtttcta
    41041 tacagctcgc cattcttatt cgtgctcaaa gaaaccatct gctaaactat attgtttccc
    41101 tgcgacaatg tcatattgga tcaattgata ttcgtcgtaa atctcttgtt gcgagcgatt
    41161 gaattgcgct tctttatacc actgtccgtt ttgatagtac agttctcgtt caaacgccgg
    41221 aagttcctgt tctaatgata aaagcagctg ataaaagcca tttgttgtct gattcgtctg
    41281 ttccattaga ttcgctgcaa aataaaacgg actggtgatt gcatcgtgag tcgtttgctt
    41341 ttctaattct cctttactgt cgaacataag aaattgtgtt tcatgcaatg tttgtttgtc
    41401 attttttgct tggatcgtat ctgaatagat ccctggaaga tgatcccccc aaaaaacaac
    41461 taaagtcctt ctagaaagat tcttcaattc ttccgtgaac tgcttcagtg ccgtgctgct
    41521 ataggaaata tcttgcagat aattctctaa gtcaagtgtc ccacttccat ctgaagtttt
    41581 tgcaggatag tctagattat catactttcc atcgtagggc atatgtgtct gcatcgtgac
    41641 taaatgaatg aactgaggtg ttttttcttc cttgagaagt gtcattactt ctttgtaagc
    41701 tgactcatca gaaatatatg gattattctc tatcgtatcc gtataggtca tcgtacgctc
    41761 gctgatgaac tggtcaaatc ctaatgtctg ataaacatcc tctcttttat acatcgaagt
    41821 attgtatgga tggatcgctg tcgtctggta tgattgtgcg tttaacgctg aaacgataga
    41881 aggaagctga tccatttttg gtacaagcat cgtgtaaggg gtggtgagtt gcgcattaaa
    41941 taacgccata gaaaaaccag tcaatgcttc aaattcgata tttgctgttc caccgccata
    42001 gttttgagaa agcatattgc cactatatgt ttgatcagct acttcataat aatctgccaa
    42061 tggctcacca gatacttcta cgccgtttaa tcgactatga tctgagaaac tttcactcat
    42121 cacgaacacg atgttcggcg actcttcctc tacagtttta tttttttcat cggctagctt
    42181 ctggtatttt tcagtgattt ctttgatctt ttctttcgaa tatccttcag gttcatccat
    42241 tggttctacc ttcagattgt acaaaaaccc accgataaaa ccagtgttat agtagttcat
    42301 tttctggctg taaggtatcc atagtgccgt tttattatat gcttttcgca acaaattatc
    42361 gggattgttg aaattactga tataccctag cagtccaatt gccacaagca gtgtcgttaa
    42421 tcggatcaat tggaatcgcc ttcctttttt caggcttcga aacatgtacc agcaaaacaa
    42481 tcctatcacg gttcctgcgg cagctaagat cactgtaaac agcatagtgc cggtcatatc
    42541 tttcagcaat ccgatttcag tgatcatctt caaatcgtct ggataaatcg gttcttgccg
    42601 ataactcatt ttcatataat ttgcaaaacc taatacaccg atggctacga catacgtaag
    42661 ggaacctaaa gggacagatc ctgctagtgc aatcagaaaa ataagaataa tcagcaagac
    42721 gagacaggct aagaagaatt tttccgtatg ccaagaaaaa gcaaatttca acgcaaggtc
    42781 tacggataga tcattttgac accactgcaa aaataaatta ctaaaaacaa caagaaaaaa
    42841 gatactgatg attgctgctg ttccttttaa aatctttttc tgtttggtcg aaagtctgct
    42901 tatatatctt ttcaaatgct tgctccttca tttctatcaa taaaagaatg ttgcttctct
    42961 tattttagtt gtacgaaatt cgaaagtaaa gaacggaaag aagaattaat gaaatccaaa
    43021 tattctcttt ctttatggcc tttctggttt ctttaataag cggacctttc gtactccaga
    43081 aattttttct agctgttgtt tcacttgatt gagctgttca aaatcagtac tgtctatgtc
    43141 aatcaatatg tagacatgct cattcttatg gttacgcgat atatctgcga tcgctagctc
    43201 gttctcattc agcagagaaa atatctctgc taatatattg gttttctttt gataaaatat
    43261 agtgaatcga aacggcgctt ggaaaaacat tcgtgcggat ggataattga ccgcttctct
    43321 aaccgtaccg aacaaaagat aatcttttaa ggcttcaact gccaaacgtc cgctgtctgc
    43381 caaagcttcc tgagtcgatc cccctagatg aggcaacata agtatacggt cattttctaa
    43441 gaactcttct tctggaaaat cagtcaagta tttagccaga tgttgttttt ccaatgcttg
    43501 cataacagca tatttatcaa caatttcact tcttcctaca ttgataagta cagcatcttt
    43561 tttcattttt tctatattcg cctgatcgat caatcctttt gtatcttctg tcaacggcag
    43621 catgactacg atgtaatccg aggtagaaag aactgtttct aaatctgctt gccgaacata
    43681 atccaactgg gcatcgcgga tagaataacc caagacatcc atccctaagc tatagcaact
    43741 tagcgctact tttgtgccta tcgctccaag accaagaaga ccgatggttt tgccttgcaa
    43801 ttctcgtccc acataagcac ttcttttgtt ttctgcctgt tctaaaatat taggacctgt
    43861 tagtgtttgt accatacgac ttgcttcgat tatcggacgg gaagataaga gcaaacagca
    43921 caaaacaagc tcttttactg cattagcatt tacacctggt gtattcatga cgatcgtccc
    43981 gttttcagaa gctttttcaa cgttgatcgt gttcacccca acaccagctc ttgaaattgc
    44041 tagcaaatct ggtgtaagcc attcatcttt cactttcgta ctacgaataa ctattccttc
    44101 ggctggcaaa tcatctatta ttttgaaata ttccccttgt aacttttcta tctcttgttc
    44161 tggaatagca tcaatcaaat gaacgtgata cataaaaccc cctccttact aacaagtatc
    44221 tcgaaatttc tgcaattaga caactgttta ccatttgact tcctagaagc aaaaagagca
    44281 gttagagcag agtaggctga gtgataaaaa taaggctatg acaagttact gccataagcc
    44341 ttattttcga gagcaaaata ttgagggtga gatatcttga taaatggtgt tccgttagct
    44401 gacattgata ataagactaa atttcaaaaa agttgaaaag caaatttcgt caatctcttc
    44461 ttatttctta aaactaagtg gctttttcac aacctctttt tatctaataa ccactgtttc
    44521 caactcaact aattttgccc acgtttcaat ttgatctgtt gttagattta gcgagacaac
    44581 ggtatgatga ccgccacctg cctgaatcca tgaatggaca ccttcatgga aattcggttt
    44641 agttttccat aacacacgtg caacaggtaa attaggtgct tcagtcgttg gttcaaaggc
    44701 ttctacttca ttgatcagca gtttataatg cgtaccgaaa tctgccatcg atacgactac
    44761 cccttctcct gctttcccat caaatacaag acgcgccgga tcttctctat ctcccattga
    44821 taaaggagaa acaacgattc ttggtttcgt cgcagcaagc gtcggatcta cttccatcat
    44881 atgggattcc aagattgctt cttggccact agctagctcg taagtataat cttccatgaa
    44941 tcctgtatct ttccctcgag ccatgatttt cagcaaccga tcaatcgctg cagttttcca
    45001 gtcgccttcc ccagcaaaac cgtatccttc tgccatcaaa cgttgtacag ctagtccagg
    45061 tagttgtttc attccataca ggtcttcaaa attagtcgta aaagcgttat agcctccttc
    45121 ttctaagaaa cggcgaatac cgatttcctg ctgagcttgt acttttacat gttcctccca
    45181 tgtcttttct tcataatcac catagtcgaa ggtatacaat tccttgtact cttcaaataa
    45241 ttgatcgact tcttcgtctg atacgcgatc gattacttgt acaagatctc cgataccaaa
    45301 ataatcaaca gtccaaccaa attgaatctg tgcttccact ttgtctcctt ctgtgacacc
    45361 cacgtttctc atgttgtcac caaaacgagc cacttttatt ccaaaacttt cattataggc
    45421 tactgcaaca tccatccaat cagcaatctc tttttggatc tcaggtcgtt tccagtagcc
    45481 aacgacgatt ttattttgtt tatttaatcg agcattgatg aacccatatt cgcggtcacc
    45541 atgagcggat tgatttaaat tcatgaaatc catatcgatc gttttccaag gaatgctttc
    45601 gttatattgc gtagctaaat gaagcaatgg tttttgtaga agctttgtcc cgcggatcca
    45661 catttttgct ggtgagaaag tatgcatcca agtgatcaca cctgctactt cttctcgata
    45721 attgacctct ttcatgattt ttgtgatcgt atcagcagta actgctaatt cttgaaggcg
    45781 gattggataa ggcaattggc cattttcatt cagttcatcg accatttctt gtgcatgttt
    45841 ctttacttct cgcagtgctt cttccccata taaatgctgt gatcctacaa caaaccaaaa
    45901 ttctttttct ccaatattca acataattag ccactccttt tatttgtttt gtccgtaata
    45961 cgcatttttt ccgtgttttc gtaaataatg tttatctaaa atccgttgag gcagctcttc
    46021 tgaaaatgaa ttgagctgtc tggtaaataa attcatcttg catacttcat ctagtacgac
    46081 tgcgttcatt accgcacttt gcgcatcttt cccccaagta aatggaccgt gtccgtgtaa
    46141 aagtacccct ggaattgcta agggatcaat tttgcgttct ttgaatgttt ctatgataac
    46201 ttttcctgtt tcatattcgt acccgctgtc gatttcctgt tgtgtcaaaa atcgagcaca
    46261 aggtaccgat ccataaaaag tatctgcatg cgtcgtcccc atcgcaggaa catccaatcc
    46321 tgcttgtgcc cagatcgtcg cccatgtcga gtgagtatgg acgattcctc cgatttgagg
    46381 aaatgaacga tataaaacag catgagtagg cgtatccgaa gagggattga gtttaccttc
    46441 aatgatatga ttgtccaagt ccacaacgac catgtcgtct gctgtcatct cttcataact
    46501 gactccgcta ggtttgataa cgaaatatcg tgtttcagga tcaaaagcgc tcacatttcc
    46561 ccatgtatat ttgacgagtc cgtgtttagg caagtcaaga tttgcttggt atacttcttc
    46621 tttcagttgt tccagcattt tctatcactc ctttttcttg caattgatga atcgctgttt
    46681 tttcaatgtc gatcccctca cggtaacgtc tcataaatat ctgatagcct gctatttctt
    46741 ctgctgaagg tgtgatggtg attccttggt cttgtccaaa cacgtcttct gccaagaagt
    46801 tttctaaaga cttcccatgt cgatccatca tataggctgc taataacgcg attccccacg
    46861 ctccaccttc tccggctgtg tccatcactg taactggtgc ctccatagca gatgctagaa
    46921 tccgctgtcc aacttctggt gttttaaaaa tccccccatg ccccactaaa cgatcaattt
    46981 ggacattctc agatttcaat atatccattc cgattctcat tgcaccaaaa gcactggcta
    47041 aatggatccg catgaaattc gctaaatcaa aacggctacc tggtgtacgt acaaataatg
    47101 gccttccttc atttactcct gtaatgtttt caccggaaaa ataaccataa gaaagtaatc
    47161 ccccgcaatc cgtatcgcct tctagcgctt tgttgaataa taccgtgaat aatttatctc
    47221 tgcttatctc catacccaaa gaattggtaa attcttcaaa aatactgacc caagcattga
    47281 tatccgaaga gcagttattc gtatgaacca tgcctactag ttctcctgaa ggtgtagtga
    47341 ccatgtcgat ttccggatgg acattttcta gttctttttc taatacgatc atagcgaagg
    47401 cggaagtacc agccgaaatg ttccctgtcc ctttggctac actgttcgta gcgaccatac
    47461 cggttcctgc atccccttct ggcggacaaa ccgggattcc tggtctcaaa tttcccgtag
    47521 ggtcgatcag ataagcccct gattcgctta acttgccagc ttcttctcct gctactagaa
    47581 ctttaggcaa aatgtgatcc aagtcaatcg ctacgtgcgc tttttcgatt aactgaccaa
    47641 aaatattttt catttcttga tggtaggttt tcgttgaagg atcgatagga aacatgcctg
    47701 atgcatctcc tacgcctagt actttctcac cagtaagctg ccaatggaca taacctgcaa
    47761 gcgttgtcaa aaaattaatt tctttcacat gttcttcttt attcaaaata gattgataca
    47821 aatgagcgat actccatctc tgcggaatat tgtattgaaa tttttccgtt aatattcgtt
    47881 ctgcttcttc agtgattgta tttcgccaag tacgaaatgg tactagtaac tgttccttgc
    47941 tatcaaatgc tagataaccg tgcatcattg cactgaaccc aatcgacccg attgtcgtaa
    48001 gattctcgcc atagatttcg aatacttctg ctgccatttt tcggtaactg acgcgcagtc
    48061 ctttccaaat atcttctaat gaataggtcc agataccatt ttctagtttg ttttcccact
    48121 cataactacc agcggcaata ggagtaaaag atgaatcaat caatacagct ttgatcctag
    48181 ttgatccaaa ctcaatccct aaagatgtac gaccatctat gacatctaac atactttgcg
    48241 catttacttc cttcataatg atttaactcc ctccttctcg tcaaacctga tcaaaccgtc
    48301 tttttagtag cgcttacaat gattattgta tacttttgta cgtacatgtc aagcagttat
    48361 ttacaagaat acaataaaaa acacttgttt tgtacgtaca taagttataa aatgaaattg
    48421 cttacaaaat ctgatcaacc gccttctcga aattctagaa ggagagaata cttatgaaaa
    48481 aagaaaaaaa agtttcctca tcgtttattt atttttttgg cgcatttggc ggcattctat
    48541 ttggatatga tattggtgtt atgaccggcg ccttaccttt tttacagcat gattggggtt
    48601 tggcaggtaa agcttctttg ataggatgga tcacgtcttc ggtcatgtta ggtgcgatcc
    48661 ttggcggctc tttatctggt cttctttctg ataagcttgg acgacgaaaa atgattcttc
    48721 tttctgcact tatttttatg gctggttctg tcctttctgc atctgcccct cacaatggtt
    48781 cttattttct gattgcagca aggattcttc taggtttagc tgttggcgct gcatctgccc
    48841 tagttcctgc ttatatgtct gaaatggctc ctgctagact aaggggaaga ttgtcaggga
    48901 tcaatcaagt gatgattgct tctggcatgc tcctttccta tgttgcagat tatttgctaa
    48961 aagggttgcc tgaaacgatg gcttggcgcg tgatgttagg gctcgctgct gtccccgcgc
    49021 tgattttatt tttcggtgtt ttagctctac cagagtcacc gcgttttctt atgcaatctg
    49081 gccgcttgga agaggcaaaa agagtattga actatattcg gacaccaaaa gaagctgaac
    49141 aggaattcga acaaatccag ctgaatgtca aacaagaaaa gacaaccgga acttcttggc
    49201 atactttgtt tttagaaaaa tatcgttcct tagtcattgc cggtattggt gtagcagtat
    49261 tccagcaatt tcaaggagct aatgctattt tttactatat tccgctgatc gtagaaaaag
    49321 cgactgggca tgcagcgagc gatgcattga tgtggccgat catccaagga atcattttag
    49381 ttgctggttc tttgctcttt ttggttattg cagacaagtt caatcggcga actttattga
    49441 aaataggcgg tagcgtgatg ggactttctt tcatcctacc agctgtttta ggaactgtac
    49501 tggatgctca tacaaattcg ttgttgattc tcttgttttt atgtatttat gtcgcatttt
    49561 attcttgcac atgggctcca ctgacatggg taatcgtagg cgaaatattc ccactagctg
    49621 tccgcgggcg agcatctggc ctagcctctt cttttaattg gattggttct ttcctagttg
    49681 gcttgctttt tcctgtgatg actgcttcta tgtctcaaga aatcgttttc ggcatttttg
    49741 ggatcatctg ctttttaggg gttttattta ttcaagagat cgtaccagaa actcgtggga
    49801 aatctttaga ggaaatcgaa caaagtgcat ctaaaaaaac ttatcctaaa cgtatttcta
    49861 tccatcattc ttaaagaggt tgtgacagaa gtggtcagct tcaagaaata agaaggaacc
    49921 gcttagtttc cagtcacaag gaacaatttc aaaaaacagc ttctcatgtt tgatgaatat
    49981 tgtgacttgt gaccagttgg taggagaaca ccgtttagtg gagaggtggt gaaagaaacg
    50041 ttcagccttg agcgatgaca aaagctctca caaacattgc gacgagtaac cgtaggagca
    50101 ataagaggcg gtaaaaaccc gtctcttatt tttcgttttc tttcactgtt tcccgttcaa
    50161 ctagtacagg ttcatagaga atatttggct gtgcctgtcc tttttcaatc gttgtgacga
    50221 tccaccgtgc ggcatcttgt cccaactttt cttttggatg cgtcaatgtt gtcaattttt
    50281 tattccctaa atggcttaaa gaagaatcgt ctgttcctac gatcgatagg tcatctggaa
    50341 tatgatagcc attttctgtc aacttatcta gcattaaact agctatttga tcgttatagc
    50401 aaatgatgcc atctaaagct tgttcttctt tcaaccgttg tactgttttt tcagcgattg
    50461 tttctcgtgt ttccgtggtg aaagtaatga tatcttccgt caaaaaacgc acattatact
    50521 gttcacacgc gctgataaaa cctttcatcc tgtattttcc ttgaagatcg tcgatttttg
    50581 tgatgaacaa cagacgctga tgtccttttt ttatcaaata ttctgctgcc ataaaaccga
    50641 ctttcacatc atctaaacaa atataagagg catttaactc ttcataagca gcattgatca
    50701 tcaccatcgg aatgccttgt tctctcaaag caacataaag tgctaagttt gggttatact
    50761 gattactttt cgtaggctca acgatcaaac catctacccc ataagcaatc attttcttca
    50821 aacactcttt ctccaattcg tgatcgttat tcgtactggc taaaagcaat gaatacccag
    50881 attctcgtaa ttcattttcg attcctcgga taatcgacgg aaaaatataa tcagagagat
    50941 atgtggtgat gacaccgatc gtttttgttc tgttcaatgg tgttttttct ttcttatagc
    51001 tgtcatctac atatgtgccc gacccttttt ctttacgcag atagccctca tttaccaaca
    51061 cagcgattgc ttgccgaatc gtatgtctgc tgacgtcata tatttcttgc agcttgaatt
    51121 ctgagggaat ggttgtattt acttcgtatt cctcacgaag gatcttttgt tttatctctt
    51181 ctgcaatatc ttgatatttt gccatttgcc ttgccccttt ttatttgtac gtacatatat
    51241 cataacgagt ctgcaaggtt tattcaagca aaaatcaagg tttcttgtgc tcataaggca
    51301 tacggaacag gttcaccaaa ttctggaaat ccttgctcat tccacgtaaa gcattgcgcg
    51361 ttcgcgtgcc gattaggatt gtctaacgga tccccttttt ggttcttctc tggtcgagca
    51421 tgatagatca aaatatcaaa gcacccgtct tctgaaaccg taaacgagtt gtgaccagga
    51481 ccaaattgct tatttttttc agaagaaacg aatactggct ccgataattt atgccatgat
    51541 tcttttaata aaagattctt gtgctcatca gcccacagca ggcccatggc atagttttca
    51601 tctgttgcac taccagaata cgtaatgaat attttcccat tgcgagtaag gactgctgga
    51661 ccttcattta ctgaaaaacc ccgtttttcc cattcatact caggtataga aaggaggatc
    51721 tgcttcccct ttaacgtcca aggattatcc atttcggata gataaagatt agaattccca
    51781 gtaatttcag gatcttgttg ggcccacaca taatatagct ttccttgaga agagaaagtt
    51841 gttccgtcta aactgaaagt ttcccttttc gtttttattt gtcccttttc tatccaatct
    51901 gtattcattg gatctatatg ctgattttca agtacgaaca tgcggtgatg atgagattca
    51961 tcacgtatag ttttatgatt gcttgcagca aagtaaatat accattttcc attgatataa
    52021 tggacttcag gtgcccagat caattcactc atcggtcctt cttcatgagc ctgccataca
    52081 atcaggcttg cagcatgttc taattcattc agtgactttg cccttcttaa ttcaatcact
    52141 tggtagcctg gaaccgatcc tgtaaaataa taataaccat ctgtatgttt atatacccaa
    52201 ggatctgccc gttgccgaac gataggatta ttataaagtg tcttcatcat aacaaatttc
    52261 ctcacatttt ctttattcta attacattcc aagagtattt ttctatccct acatttaatt
    52321 cgtcatgttt ttctattgaa aaatagttta tttcaggttt tattttttct ggttcttcac
    52381 gtgtattttt gtccaacaga tcgctactgg ttaatgtgta gtgctctcct tgatctagca
    52441 aattgaaatc atgttcaatc gttatctttt gttgttcatt tgtgcgattg atcaagaaaa
    52501 tagtcagctg attgccgtta tctacgatca cactgtccac ataaggaaca ttttttatct
    52561 tttcacaatc ataaacaggg caaatgattt gttgtttcaa tacttgacct ttagcatatt
    52621 tcgataagtg ttgcatcaca taataagttg gattcctcca tgcttgtcct ttagcatctg
    52681 tcagtattaa aggtattgtg tttactaaca atgattgaca actaatcttt acgcgatctg
    52741 cgtgtcgtaa aatagccagc cctaatgaac caaataataa tgtatcttcc atagtaaagt
    52801 aacaccaatc gatgggacta ccgacttcaa aatccctatg tcttttttcc ggttgggcat
    52861 gtacattcca ttcatcaaac gctagataca ttgttttctc acttctcaaa tatcctttta
    52921 cataatcaca agtagcaacg atttcttcaa tttgacgatc gaatcgataa gcttttgcaa
    52981 ggtatgtcgc tgtatcatca cgctcatttt taggaggttt agtcaagtct tcttcatcat
    53041 acttatcaat atagttatgc aaagctaaat aatctacatt ctcataagct tcttctaatg
    53101 cgatacgatc ccattcaggg tacgatggta aacgtggcgt agagcttcca actaagattg
    53161 tttcaattga atcatccact aacttcatga cttttgaggc ttcatttgct aacctaccat
    53221 attcataagc actcttagcc cccacttgcc atggaccgtc tagctcattc cctaaacacc
    53281 ataatttaac gccaaaaggc tttttttgcc cattttttat tcttaaatca ctccagaagg
    53341 ttccttttgg aaaattacaa tattctacta aattttttgc ttcatcaatt ccttttgttc
    53401 ctaaattcac cgtgaatatc ggtttcgctt gtacatgact gatccattta aaaaattcgt
    53461 ggagtccaaa agaatttggt tctatacctc gccatgctaa atcaattttt tttggtcgtt
    53521 gttctttcct acctacagta tccacccaat cataaccaga tgtaaagttt cctccaggat
    53581 agcgaatgac acctaattct aattccttta cataagagag gacatcttgt ctaaacccgt
    53641 caccatcagc tgtttcatgg cccggttcat aaatccctgt atagacgaca cttcccatat
    53701 gctcaacaaa tgagccaaac agccgagaac tgatctctcc taccgtaaaa tcctctctga
    53761 tttttacttt cacttcttcg tccaacagct ttccctacct ttcatttcct atagtcaaga
    53821 taatagatac aagtttccat acgccaccag caaagataaa aataaaagga gggaaaagaa
    53881 atatgagcag tacaaatcct aataacaata cgcaacaaag aatatttctc aaaaaatcaa
    53941 aaaatacttt ccatgcactt tgttcaaaaa tttgtaaaaa tgtgatatct ttgttttcag
    54001 aaatcacata tatccaggat aaaagaaaca tggaataaat aagtaaaact acatatccac
    54061 taattgaaag tacgattgtc tgttcttcta ataattgatt aacaagaaaa taaataatga
    54121 tcgttaacag actaaggaac aagttcttac caaaatgttg ctttattcct tgaaagaaaa
    54181 aataaatgat atttcctgat ccctcacctt tccattgcgc cattacataa aaaacagcac
    54241 atgtagcagg cacgatagta ataacaggca aacacacgat tacccagaga atacctaata
    54301 agatgtaatc agaaaccaca atcaactgtc cataaagttt agtctcaact attttcatct
    54361 tcctcactct ttcattccag acatagctgt tccttgaaca aaatatttct gtgcaaaaat
    54421 ataaaccact agtaatggca gcattgaaat aaccgttcca gccattacag gtccataaaa
    54481 agaaatgttc tgccctgtaa ataaagctaa ccctgctggt aaagttctca tttttgaact
    54541 acttgtcaat atcaatggat aaagcaagtc attccaacta ttcatcagcg tgaaaatccc
    54601 taatgataaa agagtaggtt tgctcaatgg caacatgatt ttcaaaaaaa tattaaactc
    54661 acttaatcca tcaatgcgtg ctgcctcttc caaagaatct ggtaaagtaa taaaaaagga
    54721 acgcatcatg aagataccaa atgcagttgt catacgagga atgatcaaac ctgcataagt
    54781 atcaagcatg ccaagcaaat tgacttcaat aaatagagga atcatgaaga cttggaatgg
    54841 gatcatcatt gtcagcaaaa cgaaatagaa caaaactgat tttcctttga aacgcatacg
    54901 agcaaatgca taccccgcaa gagaatcaaa gaatacagaa gtgatcactg tccctccagc
    54961 aaaaatcact gtatttttga tgtaatctaa taaagggatc gttgtccaaa ccttaatata
    55021 gttatctagg gtatagctat ctgcaaatat tctaggcgga aatgagataa tgtctttttc
    55081 gtgtttaaaa gaagaaataa tcagccacaa aaaaggaaac gcaataacca acaatataac
    55141 tagcatacta atcgttcgaa tgattttttt accactaact gcttttcgac tattcacagt
    55201 aactgtaatc gagctttctg gtgccataca ggtttccctc ctatttcatt tgtttttctt
    55261 tccgtgacat gtacacatta gtggagatag ctatagttgc aacaatgata aacaaaaccg
    55321 aagacagtgc tgatgcatag cccaattcat aaggtgctgt aaatcctcga ctgtaaatat
    55381 attgaacagc tgtttctgtc ttaaattgtg ggccaccagc ggttgcaaca tatacttggt
    55441 caaacacttg catcgaacca atcagattcg ttaaaataca aaaacctaag gagggaacga
    55501 tctgaggtag cgtaatgtta aaaaactgct gtcgatttgt tgcaccatcc atttgagcag
    55561 attcatacaa agagggagag attccctgaa tagcagtcaa gagaatcgtc atagtaacac
    55621 caaaattctt ccaaacagtc atgatggcta ccgtcggcat agcaagggtt ggatcgcgaa
    55681 aaaattgagg gatcggcaag ccgacttgtg aaaacaagta aggaatcgcc cctacattcg
    55741 gatcaagcaa catagaaaaa ataatcgcaa ttgacgtcaa tgaacaaata actggaatgt
    55801 aaaatgtcga acgacaaaaa cgattgaatc tggtattttt agataatgct actgctgcta
    55861 ataaaccaac gataatttgt gttggcgttt cgagtacggt gaaatataaa gaatgaatca
    55921 acgaattaac tgctcgttca tcactaaata tacgtgtgaa attttcaaaa cttacaaaat
    55981 ttcttgatgt aaagaaaata ttcaaatctg taaaactaat agcaaatgtt cctaaaagcg
    56041 gtatgacagt aaataataat aaaactaaaa aagatggagt taaaaacaaa tacgcagcac
    56101 gatcaggacg attccaatat ttggtgaatc cgttcatata gtccacctca tctttcattt
    56161 tgataaaata tacaatcttg gtttaaacga atctgaataa gatatccgaa caatcgctgt
    56221 gatttttgca ataacaccta tctgcaaata ctaggtcgat cattcagata tctgtatctt
    56281 gccaacagtc tatcctgtat aaagttctgt actatttttg cagtaattga ttgatggcat
    56341 catctgcatc gttcatgagt gtctcaggat cttctcctgc taataatttt tcaatcagtg
    56401 gattgatgat atcgttattg atcgcagaga tttgggtaaa acctggtaaa tatggctctg
    56461 catagtccat ttgtttcgat aattcagata caataggatc attttttact tcttcatctt
    56521 ttgctactga atccaagtag gcaggaaaac cattttcgat actccatttt ttaccaattt
    56581 cagttgtatt ccaatatttc aaaaactcat agacagcttc ttttttatta ttatctgtac
    56641 ttgccggaat ggcatatcct acgccatcta aaatagctgt ttttttgtct gaatcactcc
    56701 cttgtggtaa ggtagtcaca ttataatcaa tctcgtttgc tttcaatccg ttatttaacc
    56761 atggcccatt gatttcaatg gctagttgac cagcattcat taaattgtcc atttcggcgc
    56821 cagaaatcga ttctggacca gtcttgtcta catggatcat tttttggatc gcttttaacg
    56881 ttttaaggtt ctcaggagac gcaaattcag acttagtata atcatcgttc actaatttac
    56941 cgccatttgc taacatccag ttatataacg gtgcagttcc atccttattg aagacaaaac
    57001 ctgatacatt tttactacta tcagttaatt ttggtgccat ttctgtcaat tcttcccaag
    57061 tagtaggtgg ttgttcagta tctaatccag cttgtttaaa caaatcatta ttccaataca
    57121 tatacatgcc ttgtacctgc atggggacaa aatactgttt accttctact tcacctaatt
    57181 caatagccgt ttctttaaaa tcagatttat ctactccatc atattcccag aaatcgtcta
    57241 atggttgtac cgctccgttt tctacatact gtgcaaaatc tggataattt aatgcaataa
    57301 agtcaggtgc ttttttagaa gaaatcgctg taggaagttt ttcattcaaa ttggcccaag
    57361 tcatgatatc catttcaatt ttgatgtttt tatcatttgt tttattaaat tcattaacga
    57421 tttcttgcaa tatttcacca tcagatgcag taaacccatt ccaaaaagtc aaagtcactt
    57481 ctttatcaga atcagattct gctgaacttc cgccaccgca agctcctaaa atacctgttg
    57541 cagctaccaa caaagagcca attaaccatt ttttaccttt cattttttgt tcctccttca
    57601 tctgataaac gacatctgac cacattccaa gatagcggtt tcaataaaca attgattttc
    57661 ccatcttcag cttttacatt tgttgaatga cttggtttta cttgttcact tttaggaaga
    57721 ttagttgctt ttgtatcaaa tccactcatt tcagaaaaat caatcactcc ctctaatgat
    57781 aattcagaca agtgaacagt tagattcatt tcttcttctc cacggttaac tgcaaatatg
    57841 acaagttctt tgtcatttat tacggctata ctttctaaat acggcacttc ttcaaaatct
    57901 tccgtcgcat agtttgctga acgaatttgt gggcttaaca cctctccttg tccatacaca
    57961 gcagcttgca taaatggata atagatcgtc tgtctccaag cttctccgtt tgtttctgtc
    58021 ctgattggtg caataacatt taccaattga gcaagacaag caatctttac tctatctgca
    58081 tgtttcaata aagtgattaa tagacagcct aatagtaatg catcttcaaa attatagtta
    58141 tcttctagta aaggaggagc aatctgccaa ggcgctgctt gctcatcttg ttccgctgaa
    58201 tgataccaaa tattccattc atcaaaggac aggttgatct gcttcttact atgtttcttc
    58261 gccttgactg tatcgcacat cgctgtcact tcagaaatga accgatccat gtctaaggaa
    58321 cgtgcaagat aatgagaaag atcatttttt tggttgccat aatattgatg cagagacaaa
    58381 taatctattt gatcgtacgc ttgatcaaga actgttaact cccaatcacc aaatgtaggc
    58441 atttgccgat tcgaacttcc gcataaaacc acctcaattg aatcatctac gagtttcatc
    58501 gccttagccg tttcctctgc taaccgtcca tattcttccg ctgttttatg tccaatctgc
    58561 caaggaccgt ccatttcatt ccctaaacac catgtcttaa ttccaaaagg gctctcttga
    58621 ccattcgaac gccttaaatc actccaataa gttcctttag gaaaattaca atattcgact
    58681 aaattacgag ctgcatctac tccacgagta cccagattga cagccatatt gatttcggcc
    58741 cccacatgct gacaccagtt ataaaattca tgaatcccaa cttcattcgt ttcaattgtc
    58801 cgccaagcta aatccaacct tctaggtcta tttgctatgg gcccaatact gtcttcccaa
    58861 cgaaatcctg acacaaaatt tccaccagga tatctaatca tgggaatgtt caattctttt
    58921 accaagtgaa tgacgtcttg gcgaaatcca cttttattag ctgatggatg atctggctga
    58981 taaatccctt catatactgc tcgtcccata tgttcaataa atgaactgta caaccgcgga
    59041 tcaactttgc ctacacgctt gacactacta atcaacattg tggcttccat aactgacctc
    59101 ctttaattta tacatgaata atacacctcg ttgaaagcgt tgtcaataat ataaatgtta
    59161 aacttactta ttttttatcg attttaatta accaaaatat taataatgat taaaacaaat
    59221 aatttaatat aacttttatt aacaaatatg ttaatatata agaaaaacct tttaagaggt
    59281 gatatatatg caactacaaa taaacgaaga atccttaccg gtatatgaag cattagctag
    59341 taaaacaagg atcaagatca tccagctact ttccaaaaaa aagatgaatg tcaaagattt
    59401 ggcaaaagaa cttggagtga gcagtgctat cacgacgatg cacgtgaaaa agctagagga
    59461 agcaaacatc atcaaaacag aaaaagtagg acaacaaaaa atttcgagcc ttcgtgtcga
    59521 taaaatcgat atcagttttc cagaaaaaat attcaatgct tttgatacga aagaaacatc
    59581 gatacctatt ggtcactata caaattatgc aattgaacca acatgcggtt tagctacgat
    59641 ccatgatttt atcgggaaag tggatgaacc acgctatttt atggatcctc gaaggatgga
    59701 tgcccgcatt ctctggttta ccagcggatt tgtcgagtat caggcaccta attttttaaa
    59761 tacagaggat acattggaga tgttggaagt ttctgtggaa atttcttctg aatttccttt
    59821 ttcaaatgac aattggcctt cagatatcac tttcagccta aatggtgtgg agctaggaac
    59881 atggacgagc cctggagatt ttgctgatat ccgcggtaaa tatacgcctg attggtatcc
    59941 agataatctt aatcaatacg gcttgctgaa gacgatccga attaccaaac acttgacgaa
    60001 tatgaatgga gaacccttgt caaacatcac gatcaacgac atccctaaag agcaagacac
    60061 ttggcatttg cgaatcgaag tgaaagacga tgccaaacat gtggggggct gtactttatt
    60121 tggtaaagga ttcggtaatt acgatcagga tatcaaatta aaagtttact acagctgata
    60181 tttttctttt ctattttata aaacaaaaac agccaatccc tatgaaggga cggctgtttt
    60241 tgctttattg ttatttccag aaatcatcca aaacggaaat tggcaaatgt cgtttatgtt
    60301 gtgtcttgtt ccaccatgct tcgatttttt cttgtgcttg agcgggaact tcttttcctt
    60361 ctagataatc atcaatttca ttatatgtga cacctagtgc gacttcatct gcaatcattg
    60421 gtttcccatc ttcaagatct gcagtcggaa tttttgtgta taatttttcc ggtgcattca
    60481 atgcttgaag caattgtttc ccttgtcgtt tgtccaaacg gaataatggc aaaatgtcgg
    60541 ctccaccatc accaaatttc gtgaagaatc cagtaatgtt ttctgcagca tgatctgtcc
    60601 ctaatacggc tcctgctctt tctccagcta cggcatattg cgtaatcata cgttgacgtg
    60661 ctttgatatt tcctttgtta aaatcgctga ttcccacacc tgcttcgctt aatacttgta
    60721 cttgtgcatc aacagctggt ttgatattta ctcgcagact aacatcgggc tggataaagt
    60781 caagagcagc ttttgcatct tcttcatctg cttgttcacc ataaggaaga cgaacagcga
    60841 taaattgata gttttcatct tttgtttctt cacgcatttc ttccatcgtt agttgcgcca
    60901 aacggcctgc taaagtagaa tcttggccgc cgctgatacc tagtacaaaa gttttaagaa
    60961 acggatattt tttcaaatat gctttcataa agtcaatact tttacgtatt tcttcttttg
    61021 gatcaattgt tggtttgaca cctaattcat tgataatttc tgtctgcagg ctcatttatt
    61081 ctcctcctat gcttctgtat gaagttctgc tacgcttttt ttcattcgtt ccatgatagc
    61141 cattttatgg ttccagcatt cagtagacag atcgactggg tatttttgcg gattcaagtc
    61201 tcgtttatac tcttcccata gtgaatctag attttctttt gtatattctt tgatttcgtt
    61261 tagtgcaggt aattcgtaaa ctctttttcc ttcgataaag atatcttgta aaactgggcg
    61321 agctgtaaag tctcgaacag ttttattgat aaacgtatgc actggatgga acatgaagat
    61381 agcttcttct tctcgagggt cttcatccca taatgtcacg tagtctcctt cagatttccc
    61441 atcaaaattt cgagtgatgc gccacacttg ttttttccct ggagtagtta ctttttctgc
    61501 attgcttgat aacttgatcg tatccatcat ttttccttca tcatcttcga tcgatactag
    61561 cttgaatact gcaccaaggg ctggctgatc ataggctgtg atcaattttg tcccaacacc
    61621 ccatacatca attttagctt tttgcatctt taaattcaaa atcgttgctt catccaagtc
    61681 attcgaagca tagatttttg cttccgtaaa tccggctgca tctaattgtt cccgaacacg
    61741 cttagatata taggccatat ctccactgtc gatgcgtacg ccttggaagt tgattttgtc
    61801 acctaattct ttcgccacgc gaatcgcact tggcacacct gatttcaacg tatcataagt
    61861 gtcgactaag aacacgcagt ctttatgggt tttagcgtag gccataaatg cttcataatc
    61921 atttccataa gattggacta acgaatgagc atgggtcccg ctagctggta tcccaaatat
    61981 cttcccagca cgtacattgc ttgttgcatc tgctccgccg atatatgccg ctctagttcc
    62041 ccagactgct gcatccagtt cttgtgcccg tctagttcca aattccaata gtggatcttc
    62101 tccgatgaca gatttgatac gagctgcttt tgtcgcgatc aacgtttgga aattgaccat
    62161 attcaacagt gctgtttcta ctaattggca gtgcgctaaa ggcccttcca cttggattaa
    62221 tggttcattt gcaaaaacta gttctccttc acgagcagaa cgaactgttg ctttaaattc
    62281 aaaattttgt aaatactcca aaaaaccttc aggatagact tctaatccac gcaagtaagc
    62341 aatatcggaa tcattgaacg tcagattctc taaataattg actaaccgtt ccaatcctgc
    62401 aaaaaccgcg tatccgtggt taaaaggcat ttctcgaaag taacattcaa aaacagcatg
    62461 taaatcagct cgacctaatt cccagtatgt ctgcatcata ttgatttgat atagatccgt
    62521 atgcagcgtc aaactgtcgt ctgggtaagt attattcatc ttgtattact cctcttggca
    62581 agttttcatt tcctacccat tataacaaaa aaaattaata ggaaaagaaa tcttccattt
    62641 tttctgaaaa agcacatgtt ttacggatat ttcttgctaa aaatgaactc catctgctaa
    62701 aactatcctt tcttattggt atttaaccta aacgagaaag aatacttatt ttattcaatg
    62761 tatccgagac acgaagaaat tagcgttttc tatttttctt acttgataaa ataagtagat
    62821 tttcctacta caatcgtagc cgttaagata ccgatgattg aacaaatata accaaatgtc
    62881 tctacaacat aatcaatggc aaaagttttt gaagcaataa tacgtctttc cttttttgct
    62941 tcttatcccc aaagaaacgg atataactta ttatgtattg aatttttttg gaaaggcgaa
    63001 ttatgacaag atataccttg cgttgaaaaa actcgcggaa aaaatatcac acgtttttaa
    63061 aaagatacaa agtaagaaag cagctgtcaa tagaatggaa acaaaaatgg cgagacatca
    63121 atcagtctcg ccttgttttt tattgtatac attagatatg gagcaggtta ccataaagat
    63181 gattattagt cgttattaaa tagaatcctt cgtatttttt tgttctatca ctacaatatc
    63241 tttatttgga cgtttgaatt gtattccggc acgttctagc tcctgtttca ctcctacatg
    63301 gactttgaca tcttctcgtg tcatttttaa aacgtgatga atcgcatagt cgatttcttg
    63361 ccttgtgatt tttttcttcg tattgatcat cacgacttgc tttttatcat atgtgtctac
    63421 caatagcagt tgtgttcgat ctaacgaaaa taatttgtaa taattaatag ccgtattccc
    63481 aaaaaagaat tccactactt taggataaaa tgtcttaata tttaaattac tattcacagg
    63541 tgtttcgatt agcttcatct cattcctctt ttctatatag tcgatttaga tccaatctta
    63601 agttatgtta ccatctttcc tagcattaag gaatcggtta caattgaacc tttaaagaca
    63661 aaaggattta ccttttatca cttaatagta taacataatt tcgtgagaaa aaaatacaat
    63721 tttttcttga ccatctcgtt acgtaaagga ttatgcttac atttgtcagg aggaaaaata
    63781 atggagtaca caataaagaa aatgtcagag atatcaggag tcagtccgag aaccttgcgc
    63841 ttttatgatg agattggact gctaaaacca gctaggatca attcttcagg ttatcggatt
    63901 tatggcaaaa aagaagtcga tcgtctgcaa cacatcttat tttatcgaac aatggcattc
    63961 aagttggatg atatccaaga ggtcttagac aatccatctt ttgaccacca gaaagcttta
    64021 atcaagcatc agcaaatgtt gctggaaaaa cgggcacaaa tcgataccct cttgacaact
    64081 gtccaacaga cacttgatat gtacgaagga ggaagaaaaa tgagtgacaa agaaaaattt
    64141 gaagggttta aacaacaaaa gttgaaagaa aatgaagaat cctatggaca agagattcga
    64201 caaaaatacg gaaaaaaaaa ggttacagca tccaatgaaa aatggatgaa tatggataaa
    64261 gaaacgtatg aagaaatgca aactgttgaa aagcagatgt tatttgatct cactctttac
    64321 ttaaaagaac cagaagatga aagcttagca gaccgtatct tccaagctca taagaagtgg
    64381 ttctcttaca gctggcctga atatcagcca aaagcgcata aaggactagg aatgatgtac
    64441 gaagccgatg aacggttcac tgcatattat gacgagcgaa gtggtcatgg tgcagcaaaa
    64501 gctttaaatg aaattatcca aaaaaaggcg taatctttta catgatccaa caaaaaagta
    64561 cacgtaaaat gacctaataa caaaacagca gacacccaag ttagattttt atgctaactt
    64621 ttggtgtctg ctccattctt tcacaagttt tttcatccta ctttaaatat attcatcgtt
    64681 gaataaacgt ataaggtatt ttcactttat cctttttttc tgtaagaaac aattgaaatg
    64741 ccatttctcc gcaagcttcc agatggtggt cgatggttgg gaaaccaaga agctcgctga
    64801 tcagcaaatt atcgcgtcca atcaccactg gtgtcttatc ttgcagatag ctttgcaaga
    64861 tgcctgctgc aaccatatcc gaatttgtaa agacaccatc aactccaagc tctttaaaaa
    64921 agaagcctgc ttgttttcct ttctctgcat ctatacaatc ccaaaaaaca agtgattcat
    64981 caaaagatgg aaaaagttga tgacatagct gcaaagtgat ttttgagtta taacttagcc
    65041 tgccacttcg ccccaacgtg atgcccaatt gctttatacc ttgctgcttc aaataagtaa
    65101 gaccttctgt aattgattgt tctcgatcaa taaagacaca gcttgcctgt tttcccttga
    65161 tttcttcaca aaaaactaga ggtccatatt tttgatatgc caataaatca tctagtgtat
    65221 tggcacgaga cgtaatgatc aatccatcga aagctttagc tgcaaattct tcaagatacc
    65281 ttcgctcttt ttctttatga tagcccgtcg gtaaaagtgt cactttgtaa tcataatcaa
    65341 acgctgcttc aatgatccca ctgatcaact gatcaaaata cggatgattg gtataaggca
    65401 gaatcactcc aatgcttttc gtttttccat aacttaactg tctggcattt gcattaggga
    65461 tataatcgac ttctttcatg accgcaagta tttctttcct tttttcctca tctacatatg
    65521 gatgtccatt gattacacgt gaaatagtag aaactgaata acctgtgagc tttgcgatat
    65581 ctcgaatgtt tgccactgct gcttcgccac ctatacaaaa agattttctt gtaaatagtc
    65641 taccacagct ggaccgcatt caaaaaactg atacattttt ccttcatgcg aaaaactgat
    65701 ccgctgcccg ccccaatata tatcttttgt caaattttcg atttcattca ttggtatttc
    65761 ttttactata ttcctattca acccttgaaa actgtacaac gttaatacgg atcccttgaa
    65821 ctcgacaaac gcataaaaat aattgttcgt tgtgccatct gttatctcta cataagtatt
    65881 cattgcaagc cctccttttg atactttgag tatagtgtat gaaacgcgtc tcatagcaag
    65941 gagtttgttt tgccaaagaa aagttttatt cctctttcat tattaccaaa gtctcttatt
    66001 ttaaattgaa tacattataa tgatactgta aatatatctt tattattagt gaat
//
LOCUS       DACDEG010000007        61356 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.007, whole genome shotgun sequence.
ACCESSION   DACDEG010000007 DACDEG010000000
VERSION     DACDEG010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.007"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            255..686
                     /locus_tag="IV142_000485"
     CDS             255..686
                     /locus_tag="IV142_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8484172.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
     gene            693..926
                     /locus_tag="IV142_000486"
     CDS             693..926
                     /locus_tag="IV142_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8484173.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(848..1090)
                     /locus_tag="IV142_000487"
     CDS             complement(848..1090)
                     /locus_tag="IV142_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484174.1"
                     /translation="MAHQPSGSAQTECVRMPYDAPPLSCGHIYQGAIRPPGKHRNGER
                     VSRFMLPGPVRQFVTIAWMSLISRRRFMASSRHTCG"
     gene            1347..1550
                     /locus_tag="IV142_000488"
     CDS             1347..1550
                     /locus_tag="IV142_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP8484175.1"
                     /translation="MMNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVI
                     MNGNRIQIKRKQFEKIIDTLDTI"
     gene            1634..2827
                     /locus_tag="IV142_000489"
     CDS             1634..2827
                     /locus_tag="IV142_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8484176.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            3005..3235
                     /locus_tag="IV142_000490"
                     /pseudo
     CDS             3005..3235
                     /locus_tag="IV142_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
     gene            3363..4046
                     /locus_tag="IV142_000491"
     CDS             3363..4046
                     /locus_tag="IV142_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP8484177.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            4355..4428
                     /locus_tag="IV142_000492"
     tRNA            4355..4428
                     /locus_tag="IV142_000492"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4389..4391,aa:Arg,seq:tct)
     gene            4587..4946
                     /locus_tag="IV142_000493"
     CDS             4587..4946
                     /locus_tag="IV142_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP8484178.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            5069..6700
                     /locus_tag="IV142_000494"
     CDS             5069..6700
                     /locus_tag="IV142_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP8484179.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(6762..7403)
                     /locus_tag="IV142_000495"
     CDS             complement(6762..7403)
                     /locus_tag="IV142_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP8484180.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            7685..7993
                     /gene="rplU"
                     /locus_tag="IV142_000496"
     CDS             7685..7993
                     /gene="rplU"
                     /locus_tag="IV142_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP8484181.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            8020..8364
                     /locus_tag="IV142_000497"
     CDS             8020..8364
                     /locus_tag="IV142_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP8484182.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            8377..8670
                     /gene="rpmA"
                     /locus_tag="IV142_000498"
     CDS             8377..8670
                     /gene="rpmA"
                     /locus_tag="IV142_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP8484183.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            9077..11911
                     /locus_tag="IV142_000499"
     CDS             9077..11911
                     /locus_tag="IV142_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8484184.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            12102..12593
                     /locus_tag="IV142_000500"
     CDS             12102..12593
                     /locus_tag="IV142_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP8484185.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            12696..13685
                     /gene="manX"
                     /locus_tag="IV142_000501"
     CDS             12696..13685
                     /gene="manX"
                     /locus_tag="IV142_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP8484186.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            13718..14521
                     /locus_tag="IV142_000502"
     CDS             13718..14521
                     /locus_tag="IV142_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP8484187.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            14542..15456
                     /locus_tag="IV142_000503"
     CDS             14542..15456
                     /locus_tag="IV142_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP8484188.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFSPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            15539..15913
                     /locus_tag="IV142_000504"
     CDS             15539..15913
                     /locus_tag="IV142_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP8484189.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            16052..16297
                     /locus_tag="IV142_000505"
     CDS             16052..16297
                     /locus_tag="IV142_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8484190.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            16469..17533
                     /locus_tag="IV142_000506"
     CDS             16469..17533
                     /locus_tag="IV142_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP8484191.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            17779..18204
                     /locus_tag="IV142_000507"
     CDS             17779..18204
                     /locus_tag="IV142_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8484192.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            18197..18646
                     /gene="nusB"
                     /locus_tag="IV142_000508"
     CDS             18197..18646
                     /gene="nusB"
                     /locus_tag="IV142_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP8484193.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            18743..19597
                     /locus_tag="IV142_000509"
     CDS             18743..19597
                     /locus_tag="IV142_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP8484194.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            19598..20935
                     /locus_tag="IV142_000510"
     CDS             19598..20935
                     /locus_tag="IV142_000510"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP8484195.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            20940..21164
                     /locus_tag="IV142_000511"
     CDS             20940..21164
                     /locus_tag="IV142_000511"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP8484196.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            21166..22056
                     /locus_tag="IV142_000512"
     CDS             21166..22056
                     /locus_tag="IV142_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP8484197.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            22065..22883
                     /locus_tag="IV142_000513"
     CDS             22065..22883
                     /locus_tag="IV142_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP8484198.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            22990..23439
                     /locus_tag="IV142_000514"
     CDS             22990..23439
                     /locus_tag="IV142_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP8484199.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            23457..25139
                     /gene="recN"
                     /locus_tag="IV142_000515"
     CDS             23457..25139
                     /gene="recN"
                     /locus_tag="IV142_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP8484200.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(25194..25310)
                     /locus_tag="IV142_000516"
     CDS             complement(25194..25310)
                     /locus_tag="IV142_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP8484201.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(25383..26339)
                     /locus_tag="IV142_000517"
     CDS             complement(25383..26339)
                     /locus_tag="IV142_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8484202.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            26510..26878
                     /locus_tag="IV142_000518"
     CDS             26510..26878
                     /locus_tag="IV142_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP8484203.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            27048..27479
                     /gene="mraZ"
                     /locus_tag="IV142_000519"
     CDS             27048..27479
                     /gene="mraZ"
                     /locus_tag="IV142_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP8484204.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            27500..28459
                     /gene="rsmH"
                     /locus_tag="IV142_000520"
     CDS             27500..28459
                     /gene="rsmH"
                     /locus_tag="IV142_000520"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP8484205.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            28464..28865
                     /gene="ftsL"
                     /locus_tag="IV142_000521"
     CDS             28464..28865
                     /gene="ftsL"
                     /locus_tag="IV142_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP8484206.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            28866..31058
                     /locus_tag="IV142_000522"
     CDS             28866..31058
                     /locus_tag="IV142_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP8484207.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            31084..32046
                     /locus_tag="IV142_000523"
     CDS             31084..32046
                     /locus_tag="IV142_000523"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP8484208.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            32060..33421
                     /locus_tag="IV142_000524"
     CDS             32060..33421
                     /locus_tag="IV142_000524"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP8484209.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            33447..34535
                     /gene="murG"
                     /locus_tag="IV142_000525"
     CDS             33447..34535
                     /gene="murG"
                     /locus_tag="IV142_000525"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP8484210.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            34550..35770
                     /locus_tag="IV142_000526"
     CDS             34550..35770
                     /locus_tag="IV142_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP8484211.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            35926..37251
                     /gene="ftsA"
                     /locus_tag="IV142_000527"
     CDS             35926..37251
                     /gene="ftsA"
                     /locus_tag="IV142_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP8484212.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            37274..38515
                     /gene="ftsZ"
                     /locus_tag="IV142_000528"
     CDS             37274..38515
                     /gene="ftsZ"
                     /locus_tag="IV142_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP8484213.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            38532..39209
                     /locus_tag="IV142_000529"
     CDS             38532..39209
                     /locus_tag="IV142_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP8484214.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            39223..39822
                     /locus_tag="IV142_000530"
     CDS             39223..39822
                     /locus_tag="IV142_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP8484215.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            39837..40124
                     /locus_tag="IV142_000531"
     CDS             39837..40124
                     /locus_tag="IV142_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP8484216.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            40173..40955
                     /locus_tag="IV142_000532"
     CDS             40173..40955
                     /locus_tag="IV142_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP8484217.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            40976..41683
                     /locus_tag="IV142_000533"
     CDS             40976..41683
                     /locus_tag="IV142_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP8484218.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            41945..44743
                     /gene="ileS"
                     /locus_tag="IV142_000534"
     CDS             41945..44743
                     /gene="ileS"
                     /locus_tag="IV142_000534"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP8484219.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(44792..46318)
                     /locus_tag="IV142_000535"
     CDS             complement(44792..46318)
                     /locus_tag="IV142_000535"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP8484220.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(46333..46980)
                     /locus_tag="IV142_000536"
     CDS             complement(46333..46980)
                     /locus_tag="IV142_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP8484221.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(47164..47493)
                     /locus_tag="IV142_000537"
     CDS             complement(47164..47493)
                     /locus_tag="IV142_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP8484222.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            47670..48398
                     /gene="sfsA"
                     /locus_tag="IV142_000538"
     CDS             47670..48398
                     /gene="sfsA"
                     /locus_tag="IV142_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP8484223.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            48414..49427
                     /locus_tag="IV142_000539"
     CDS             48414..49427
                     /locus_tag="IV142_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8484224.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            49427..50704
                     /locus_tag="IV142_000540"
     CDS             49427..50704
                     /locus_tag="IV142_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8484225.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            50767..53469
                     /locus_tag="IV142_000541"
     CDS             50767..53469
                     /locus_tag="IV142_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8484226.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            53621..53938
                     /locus_tag="IV142_000542"
     CDS             53621..53938
                     /locus_tag="IV142_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8484227.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            53968..54288
                     /locus_tag="IV142_000543"
     CDS             53968..54288
                     /locus_tag="IV142_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8484228.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            54396..55856
                     /locus_tag="IV142_000544"
     CDS             54396..55856
                     /locus_tag="IV142_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8484229.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            55924..56145
                     /locus_tag="IV142_000545"
     CDS             55924..56145
                     /locus_tag="IV142_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484230.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            56176..56358
                     /locus_tag="IV142_000546"
     CDS             56176..56358
                     /locus_tag="IV142_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP8484231.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            56358..56771
                     /locus_tag="IV142_000547"
     CDS             56358..56771
                     /locus_tag="IV142_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP8484232.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            56894..58075
                     /locus_tag="IV142_000548"
     CDS             56894..58075
                     /locus_tag="IV142_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8484233.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            58464..58537
                     /locus_tag="IV142_000549"
     tRNA            58464..58537
                     /locus_tag="IV142_000549"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58498..58500,aa:Arg,seq:cct)
     gene            complement(58606..59745)
                     /locus_tag="IV142_000550"
     CDS             complement(58606..59745)
                     /locus_tag="IV142_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8484234.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(60044..60679)
                     /locus_tag="IV142_000551"
     CDS             complement(60044..60679)
                     /locus_tag="IV142_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484235.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINSASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(60792..>61356)
                     /locus_tag="IV142_000552"
     CDS             complement(60792..>61356)
                     /locus_tag="IV142_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAP8484236.1"
                     /translation="IKKTNKKTPMILLIIGIIFTTVGFSLSSSNEANDNNKQESSSSD
                     TQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSYYSITPATEQNTAFTKYISGKTYTF
                     EGTVIESMTTRIAIIADKEYDDKSWNDISATPKVSYVIFAKDLNNADTFTKGDKVTFT
                     GEISSRGSNIEKSYAQWDMINSKVSKK"
BASE COUNT        19983 a        10568 c        13230 g        17575 t
ORIGIN      
        1 cacagggcta ttccggttat gagtatgtac tataccatgt aaatcagcag catagcaaag
       61 cattttctcg cccggtaggt ttgcgacctg ccgggcgatt tttgtcgtct tttgcggccc
      121 ggctgtcccc aaaaaaattt tttgagaaat tttcaaaaag gaggtattta gcgggtagct
      181 ggcggccttg tcaaagtctt gttagcggaa agggagagaa ccacctaagt cccccacaga
      241 aagggggtga gaagatggat gctatccccc gcgactatga agcgcggtgc atgttcgatg
      301 ctttttgcaa gacggtactg cgtaacgagg ccaaaagcta cttggctgaa atgaagcgcc
      361 gccgtgaccg tgaagtgtca ctaagctctt tgtcacaggc agatttggac aagctctgta
      421 cagtagatca ctaccccagc gacagcttta cgttttcgtc gcatggctat gacctacata
      481 tcaacaatga gcttgttgcc gaagcctttg ccgcgctgcc cagcatggag cagagtattt
      541 taatcctgca ctgtgttctg gatatggcag atggtgagat cggcggcctt gtgggaatgt
      601 cccggagtgc cgttcagcgg cacaggacaa ataccttatc ggagctgcgg aaacagctaa
      661 aggcgttgat gccgaaggga ggttagacgc ggatgaagaa cccgaactac aagggcattg
      721 agcttttgcc cctttcggtg atcgaggccg cccgcgctgg ggatgccgga gccgtagagc
      781 gcgttttgca gtattacagc ggttacatga acaagctgtg tacccggacg ctctatgacg
      841 aaaacggcta tccgcatgtg tgtctggacg aggccatgaa gcgccgtctg gaaatcaagc
      901 tcatccacgc tatcgtcacg aactgacgaa ccggccccgg cagcatgaaa cggcttaccc
      961 tttccccgtt tcggtgtttt cccggtggcc tgatcgcacc ttgataaata tgcccgcaag
     1021 ataacggcgg cgcatcatag ggcatacgga cacattctgt ctgtgccgag ccggacggct
     1081 ggtgcgccat gatacttttc cctacgggaa aagcggagcg aggaacacca cgccgcaatg
     1141 tgagcgtggc ggcttgcggg cgacgacgta caggcaggac tatgatactc ccttatagcc
     1201 acagtccgag cgttaaaagc gtcgcaggca atgggtaggg ccgcgtgaga accacgcggg
     1261 ggtgaaagtc ccgtggagct gtgccagcag ccgtccgtct atgcccctaa atcacacaaa
     1321 cctttgaatt tgtgaaaggg ggctgcatta tgaaccatga tgtgcctatt tgggaaaaat
     1381 acacgctgac gattgaggaa gcgtctaaat atttccgtat cggagaaaac aaattgcgta
     1441 agctggccga ggaaaatccg gcctctggct gggtcattat gaatggcaac cgcatccaga
     1501 tcaaacgcaa gcagtttgag aaaataattg acactttgga cacaatctaa tagtgaaaaa
     1561 gggccgcaga tatgttataa taatttataa gcatatatgc ggctctttcc gtcatggaaa
     1621 ggagcatgac gagatgtcag aaaaaagacg agacagcaaa aatcgcgttt tgcggtctgg
     1681 agagagccag agaaaagacg gacgatatgc ttacaaatat gttgatacct ttggaaaacc
     1741 gcagtttgtt tactcatgga agttagtgcc tacggataag accccggcag gtaaacggga
     1801 ggatatttca ctccgggaaa aggagaaaga aatccagaaa gaccttgacg acgggataga
     1861 caccatcggc aagaaaatga ccgtctgcca gctctacgcc aagcagatca ggcaccgggg
     1921 caatgtgaag cacaacacca aactgggccg ggaacggctg atgcgtattc tggaacaaga
     1981 tcggctgggg agctgcccga ttgacagtgt gaagctgtcc gacgcaaagg aatgggcgct
     2041 tcggatgaaa gaaaagggcc tttcttataa gaccatcaat aacgacaagc gttccctgaa
     2101 agcggctttt tacaccgcca tacaggacga ttgcatccga aaaaacccgt ttgacttcca
     2161 gcttagtgac gttctggacg acgatacgga accaaaggtg cctcttacac cagcacagga
     2221 agaaagtttt ctgtccttta ttcaaggtga taaggtttac caaaagcatt atgacgcaat
     2281 cgttattctg ctggggacag ggcttcgcat ttcggaactg tgcggactga cggacaaaga
     2341 tttggatttt gaaaaccggg taatcatcgt gagccaccag cttttgagaa ataccggcgt
     2401 cggttactat attgacgaac ccaagaccca aagcggcgtc agaaaaatcc ccatgaatga
     2461 ggaagtgtat caggcgttcc agcgagtgat aaaaaaccgc aaaggtgcaa agccgttcat
     2521 tattgacggg tatgccaatt tcctgtttct caaacagaac ggctatccca tgacggcggt
     2581 agattatggc gggatgtttg gcaggcttgt gaagaagtac aacaagagcc atgaagaagc
     2641 gttgccgaaa acgacgacgc cccatgctat gcgtcataca ttctgcaccc ggttagccaa
     2701 tgcgggaatg aaccccaaag ccttgcagta cattatggga cattccaata tcaccatgac
     2761 gctgaactat tatgcacacg ccacattcga ttccgcaagg gcagaaatgg aacggctggc
     2821 agcctgacac aaggccggtt ttactactcc atttactact tttgaggggg aaatggtgag
     2881 aggtaataag aacatttgta agtatctaac aatatcaaaa atgccggaaa agcccggaaa
     2941 cacgggctat accgacattc aagaacttct aaaaagataa tctaaaatcc catataattt
     3001 ttcctcaaaa gctgatacaa agacttatat tgagcctgga gtagtaaaaa agagaaatat
     3061 tatcggtagt cccatctttc agattccata tattggacta gtcctttcaa aaacggcatt
     3121 tgcatggatt caggggttat tctttacgat tgctttttgc ctaacaggaa tcacagcatg
     3181 gaataccttt gtagagttga aaaagaagcg gagacgaaca cagaaatttt tgtaagaatg
     3241 ttaaaaacat aaatgtattc cagagatcca acgactcttc tgaacatgaa acacgagaaa
     3301 acactcatgc agcttgtaga tttagtcagt tgttcgtata atgtgtgaag aggaggagta
     3361 gtatgcgcaa tgaaatgatg catcgtcctg tagtcaaaga agaactattg gattttatgc
     3421 gtacccagca aaaaaaatta ccgggtgagc taggcaaact ggaagaagaa gctcatgtag
     3481 cagaagtacc agttattccc catgaaacag ttgtattttt gaaattttta cttggacagc
     3541 ttcagcctga acgaatcttg gagatcggag cagctatcgg tttttcatca agtgtcatgg
     3601 ccacgacaat cggagaaaac gctcatgtta cgacaattga tcgtttcgat gtcatgatcg
     3661 agaaagctaa aaaaaactat gaacgtctag gactgacaga taaagtgact ttgcttgaag
     3721 gtcaagcagc agatatcttg ccggagttgt ctggtccgta tgactttatt tttatggata
     3781 gtgcaaaatc aaaatatatt gaatttttac ctgaatgctt acgtttgtta agaaaaggtg
     3841 gcgttttgat ggtagatgac atcttccaag gaggaacgat cttgctccca gatgaagaga
     3901 ttccccgtgg caaacgagct atccatcgta aattaaatga atttcttcgt gttgtgatgg
     3961 atcatcccga tttgacatcg acaatcttgc cattaggtga tggtgtgatc ttaatgacaa
     4021 aggaatcaga aacaatcgag ttgtgataca tgattatggc agcctgtaga taatcgattg
     4081 aatcgagggc tcaattcgaa cttgatgtaa aagtttctat atttgtaagt caattaaagg
     4141 atacaggaaa tctaaataaa caggagtagc atgaagtcaa tgtagctcct gttttcaatt
     4201 gataacctta gaacagctga aggagaatgt ctcgttttaa aagattgatt tagtagttgg
     4261 gccaaaataa ttcattattt gctaattaaa taactatttc gtattgacag aagcaattta
     4321 attatctata attattgatg ttccgctgat tcttgtcata gtagctcagc tggatagagc
     4381 atccgccttc taagcggacg gtcgggggtt cgaatccctc ctgtgacgct aaaaaaccgt
     4441 tagaaactga ggtttctaac ggtttttctt tgttttttgt gccaatattg tttgttttca
     4501 aaggcaaata aaataaaaaa acatccttat cgagtacaat tataaggagc aaaaaaataa
     4561 aactcatcag aaaagaggaa gaagatatga cgatactatc tagaatttta gtgactctag
     4621 ttgcaattga atttttttat atcatgtata tcgaaacggt gagaactgat tctgacacga
     4681 caagtagggt ttttaaaatg agtaaagaag agttaagcag aaaatctgtt caaacattgt
     4741 ttaagaacca aggagtgtac aatggactat taggagtggg actactttat ggagcttatc
     4801 tttcttccgc atctaaagaa atcacaagta tgctattaat atctattttt tttgttgcat
     4861 tatatggcag tttgactagc gatcgttgga ttttactcaa acaaggtggg ctagctctgt
     4921 tggcattgct ttctattttc ttataaatat agaaaaaata agaatttatc taacaggaaa
     4981 ctttgtgaaa aatattactt tatctcatga aatatgatat tctaatgaat ggtattttag
     5041 aaaatttcat gaaaaaggag ttagataggt gttcaaaaat tatgtttcct cactaaaaaa
     5101 agaattttca ggttatgatc tttcaagatt tcagaaagat cttttggctg ggatcacagt
     5161 agctgctgta gcattgcctt tagccttggc ttttggggta tcaagcggag ctgatgcggc
     5221 cgcaggtttg attacggcta tcattgccgg tatcgttatt ggcggtttat caggcggttt
     5281 ttatcaaata tcaggtccga caggtgcgat ggcagctatc ttgatgtcta ttgcggcaac
     5341 aaagggaatg caaggtgtat tgcttgctac ttttttagcg ggtctatttt tacttgcagc
     5401 aggacttttc cgattaggaa cactgacttc ttttatccca gcacccgtaa ttacggggtt
     5461 tacttcaggt attgctatca ttattgcatt aggtcagatc gataatttat ttggtgttca
     5521 ctcggaaggt acaaacgtta ttgaaaaact aagtagttat caaacgatag gatttccgat
     5581 aaacacagcc tctttgatca tgggagggct agtcatttta gggatgttta tctatccaaa
     5641 aaaatgggcg aaacgaatgc ctagttcgct gctggctatc attttggcaa cagcagttat
     5701 gctattgttc catttaccag tagcaactgt gggtaagatt ccacaaacat tgatcagtag
     5761 taatcgtttg aatatgggag attttagctt ttctgccttg caacaagtgg cagttccagc
     5821 tattagcatt gctttattag gaatgattga aagcttgcta tgtggtgctt cggctggccg
     5881 aatggcaaat aagcccttag atagcaatca agaattaatg gcgcaaggaa ttggaaatct
     5941 gttgcttcct ttctttggcg ggattcctgc tactgcggcc atcgcccgta caagcgtggc
     6001 catcaaatct ggtgcacaaa ctagattagc tggtatgata catgcagttg tcttactgct
     6061 ctccatgctt gtttttgcgc cagtgatgtc acagattcca atgccagcac ttgcaggtgt
     6121 tttgattgtg acggcttggc gtatgaatga atgggaaacg atcaaggagc tatttactaa
     6181 taaatattgg tcagcagttc tattgtttat ccttacgatg atttgtacag ttatctttga
     6241 tttaagtatt gctatcgtga tcggggtgat cggcggctgt gtattttttg ttgtcaaaag
     6301 cgctgctatc acgatttcat tagaaacgat cgattggcaa agaatggggc tgccagaaac
     6361 ggaaaagctg aataactgga cagtagtgta tattagtggt ccactgtttt ttatgtcttc
     6421 agaaaaactc aaaaataaat tggaagaact aaatcatcac gatggtgtca ttttctccat
     6481 gcgaggcgta ccaagtattg atttgacggc acgtgcagtt tttgatgaat ttcaagaaaa
     6541 agcagagcaa agaggacaaa cgattatcta tacttccttg cagccagaag tagaaaaaca
     6601 actttctcac ttgtgggaac atacattcac gcatcaacat caaacagttg ctcatgcgtt
     6661 gacagcttta catgcgaaat atactgcaat tagccattaa aacaatgaaa aagaacagac
     6721 aagactgtaa actgaagatt ttatggtctt gtctgttctt tttaatattg ctgtaaaagt
     6781 gaagctagta cttgccagtc ttgatggata acaggtacaa gggagcgatt gtagaaaata
     6841 gctgctggat gaaaaagtgg aagaattcga tagttttttt ctgaccattc gtaggcatcc
     6901 ttttgttctg ttaattgtaa gacaggccct gtatagagtt gaccatgatt tttggtaacc
     6961 gtatattctt tgccaagtaa acgttgaaga ccaatgtttc caagtgtcgc aattaatttt
     7021 ggctggacct tcatcagttc gtaatccaat attggtgcat gcgcaaatac ttcggaacga
     7081 gtaggtgtcc gatttggata aacagtttct gactgttgtg tccgtttatt gatccgatga
     7141 gtcacgcgat acgggcggct ccttacagca ctggtgatat acacctcttc tcgtgttaag
     7201 ccagttgaag aaagtgcctg catcaattct tttccagctt gaccgctaaa aggaacatgc
     7261 gtatcaatct ctgttttccc tggggcttct cccacgagca tcagtttagg atgaagaggt
     7321 ccttgcccag cgataaatcc ttctacagga cggccgttca ttcttaaaga agcttcttgt
     7381 atcagttctt ttgggtattc catttatgct cctccttttt cttttattat aagaataaaa
     7441 gaaacacaat gcgactattt tatttggtag aactgtgagg atagaaaagc agaagtattg
     7501 cttgaatttt tgttttactt tttgatttta aatttagttg actaacggtt gctgttctgg
     7561 taagctagta ctgttgtaat tgtggactcc acagctacaa ccgctcagaa caagtaataa
     7621 gcacagcata tgctgtcact tcggatggcg agtctaagta tatcagaagg aggtgcttta
     7681 tagcatgtac gcaattatta aaactggtgg taaacaagta aaagttgaag tgggacaagc
     7741 aatctatgtt gaaaaacttg acgttgaagc aggagaaaaa gttgtttttg acgaagttat
     7801 cttagtaggc ggagaatcta caaaagtagg cgctccaact gttgcaggtg caactgttga
     7861 aggtactgtt gaaaaacacg gcaaacaaaa gaaagtcgtt actttcaaat acaaacctaa
     7921 aaaacattca caccgtaaac aaggtcatcg tcaaccatat acaaaagtgg ttatcgacgc
     7981 gatcaacgct taatagcgtt tgagaaagaa ggcctatata tgatcaaagg aacatttaag
     8041 cgaaatgacg caggtcagat cgtttcattc actttgaccg ggcatgctga tgctggtcct
     8101 tatggaagtg atatcgtttg tgcgggtgtt tctgcgttag cgatcagcac tgtcaacggg
     8161 attgcctcat tagccggttt tgagccgatt gttgaaatga atgaagaaga aggcggttat
     8221 ctttataccg aagtcacttc aggaatgaca caagaacaaa acaatattgc tgagattcta
     8281 ttagaaaatc ttttattagg attacaatcg atagaagctg aaaactcaga atatatccaa
     8341 attaaaacaa ttaatgataa ataggaggtg cagaccatgt tgttatctat gaatctacaa
     8401 ttatttgccc acaaaaaagg tggcggttct acatctaacg gccgtgactc agaatcaaaa
     8461 cgtctaggtg ctaaacgtgc tgatggacaa acagttacag gtggatcaat tctttaccgt
     8521 caacgcggta caaaaatcta tccaggtgta aacgtgggta tcggtggaga cgacacttta
     8581 tttgcaaaag ttgatggcgt agtacgtttc gaacgtaaag gccgtaacaa aaaacaagtg
     8641 tctgtttatc cagtagctca agaagcttaa ttgaatctta tgattaaaga ggttgtgaca
     8701 agacttttgt cacagccttt tttgtttata aaaataaaag ttgtagtgga ggctacgaag
     8761 tttactttgt ctttttacac ttaatatttt tttgccaatt tggaatcagg atgttttgat
     8821 aactatcgtt ttcaattgat ggaagctata ttgtttgcta tcagtgaaat aattatatgg
     8881 taaacagtgt tctttttctt gtttccatta taaaaatcgt atcgggattt ttcgttcttt
     8941 taaattgaac atttctatct atccgcgtgt tatcaacaaa acaaaatcag gtaaatagtt
     9001 gacgaaacag aaataaatac atagtattat gttttgaagc ctaagtacaa tagtctatag
     9061 aaaaggagcg ataccattgg caaaacgcat tgaccgaatt tatatgtatg tcaaagaaaa
     9121 aaccgctcat ttaacaaagg cagaatatga tcaaggagta acaacacagg aaattgctga
     9181 agtgttaggg attcagcgaa caaatagcag taaagatctg aatcagctag tgcgcgaagg
     9241 aaaactgata aagacagacg gacgaccagt acgttacata tatcaaacac tcgttacgca
     9301 cgtaaagcca gttacaaaac atgtagtcag ttacaaagaa acgccggtct ctgaagaaaa
     9361 aaatacactc caaatagata ctaaggatat ctttgcaaaa atcattggag ctaacggcag
     9421 catgaaaaat tcagtggaac aggcaaaagc tgcgattttg tatccgccaa gaggattaaa
     9481 ttgtttgatt acaggaccta ccggatcagg aaagacttat tttgcacacg cgatgtatca
     9541 ttttgcaaaa gcgcatcatg taatagatga ggaaacggaa ctgattgttt tcaattgtgc
     9601 tgattatgcc aataaccctg agcttttgat gagtcattta tttgggtacg caaaaggtgc
     9661 ttttacaggc gcagatgaag aaaaaatggg gattatcgat caagcggatg gcggtatgct
     9721 gtttttggat gagattcacc ggttgccgcc tgaaggtcaa gaaatgatct tttactttat
     9781 ggatcatgga acttacagtc gtcttgggga aacaacgaaa tcccatgaag caaatgtacg
     9841 gatcgtaggt gcgaccacag aagacccagg ttcttctcta ttagaaactt ttgttcggcg
     9901 tatcccaatc aatatcaaac ttccggcttt tgaaaaacgt cctgcaaatg agaaagtcga
     9961 tttagtaaaa atcatgattg cccacgaagc aaaccgcacg cagcgaaaga tttctttgac
    10021 agaagatgta gcaaaagcat tgatcggcag tgtgacctat ggaaatatag gacaattgaa
    10081 gtcaaatatc cagttagtat gtgcacgagg atttctaaat catatgcaat cacccgaaat
    10141 ctcgatcaca atcgatgatt tgacagaagg gattcgtagc ggattgattc aattagcaag
    10201 caaccgcagt gcaatggcag agctttctaa aatattggag ccaaagataa ctgtagcacc
    10261 aaatgatacg atcatgaaaa tccaatcgga ctcctatgag ttaccttata atctatatga
    10321 tatcatcgga gataaagcag ctttactgaa gtcggatggt ttggatcaag aagcaatcaa
    10381 tcatttcatt tctacagata tcaacatcca tctgaagtct ttctataaag accatggttt
    10441 ttcttttaat gcagacagca aattagcaga atttgtagat ccgaaaatca tagaagtgac
    10501 caatcagatt tatgtaatgg tcaaaagatc tttaccatat gagtttcagc aaaactttat
    10561 ttatgctatg agcttgcata tcagttcttt tttgaaaaaa atcaacttag gagaagaacg
    10621 gcatacgaat gataacatcc gtgagatggc ttttgatttt ccagatgaat atgctgttgc
    10681 gaaagaagtg cgtagttata tcgaaacgta ttttcaagtg gagattccag aaagtgaaga
    10741 ctattacttg accgttctac tcgtctcgtt acgggcaaat caagcaagcg gcagaatcgg
    10801 tgtagtcgtt gcagctcacg gaaacagcac agcaagcagt atggtccaag ttgtccaaca
    10861 gttgttagat gcagagaacg tacgagcagt agacatgcct ttggatatgg atccgaaaac
    10921 agcattggac cgaattgaac gcaatgtaca agaagtagat gaaggaagcg gggtcatact
    10981 cttagtagat atggggtccc ttgcgtcgtt taattcgcaa attcaaagag acactggaat
    11041 tccagtacgt actgttgata tggtaacaac ttctctagtt ttggaaactg tacgaaaggt
    11101 ctcagtatta gggacagatc tagatatgtt gcatgaatca ttgaaaaatt ttcgaggtta
    11161 cgcagaaata tcttctgtag aatctgttca ggaagcaaaa cctattcata agaaaaaagc
    11221 tgttttggct atctgcgcat ctggcgaagg tacagcacaa agaatcaaag aattgatcga
    11281 acgagcggta aacaagcgtc aagaaacaga attagaagtt ctagctttgt cgattgtgga
    11341 agtgaaagaa gaactgccag ttatccaaaa agaatatcag atcatagcta ctactgggat
    11401 tactgatttg aatatccaag ccccatttat tcctttagaa cgttttattg atcaaaatat
    11461 cgaagtcatt ttggatcagt tgctgatgga atcagaattg gaggaaactg aattcatctc
    11521 cctagatgaa gagtcagcta aaaatacgtg tgttgagttt atctctgata acttcatctt
    11581 tattaatggt agtaagttaa tcgatccaat gtggcaattt tctacacaaa tctctcagac
    11641 gacgggaatc ggcgatgaag aatatggatt caaaatcaac ctagtgatgc acacagcagg
    11701 aatgatcgaa cgtattattc ggaatgaacc attgactgtg gaagaaaacg aattgacaaa
    11761 tacaacgaat gatcctcttt atagccaatt ggcagcctca gttgttctgt tggaagatca
    11821 gataaaagta aaagtgccga tagaagaaat gtattatctg ctacgtctag tccataatca
    11881 gttagataaa aaagaataca ctgtgccatg aatacacaaa aacgatacac ttttatagtg
    11941 tatcgttttt gtgtattggt taagaatcct ttaaattcaa ttagtgtatc gtaaaaaagt
    12001 tggcacgctt tttgcttata gtagtgtgtg ctaggaggtg gttgttcaca cagaaatgat
    12061 aggggtttct gtgaagtagt gaggaagaag gagttttgca gatggatatt cgattggcac
    12121 gtatagatga tcgtttaatc cacgggcaag tcgcaacagt ttggtcgaag caaacaggtg
    12181 ttgaacgtat cattgtgatc agcgatgtag tcgctcaaga taaattgaga aagtttctac
    12241 taaaagaagc tgcaccgccg gggatcacag caaatgtaat aaccgtgcag aaaatgattc
    12301 aaatctatca tagtggatta ttatcacgag caaaagtgat gcttttattc accaatcccc
    12361 aagatgttga agcggttgtc cgctgcggtg tacagttgaa gtcgttgaat atcggcggta
    12421 tgagttttac tgaaggcaaa tcaatgatta ccaattttgt ttcagtaaat caacaagata
    12481 ttgattcctt tcgttacttg aacagtcaag aagtggagtt agagattcga aaagtacctg
    12541 ctgacaggaa aatccttttg atggaactac tagcgaaaat caaaaaggtg tagcaagaat
    12601 ggttgttgga aaaggtcaaa taattctgtt gtaatggtta gcaattatta atatttttga
    12661 atttgttaca acaaaagtaa ataggaggta taaatatggt agggattatc ctagcaagtc
    12721 atggacaatt tgctgaagga atcttacaat ccggttcaat gatctttggc gaacaagaaa
    12781 acgtcaaagc cgtgattttg aaaccaagtg aaggtccaga tgacttgaga gccaaattgg
    12841 aagaagcggt tgcttctttt gataatcaag acgaagtgtt attcttagtt gatttatggg
    12901 gcggaacacc gttcaatcaa tcaaacacat tattcgaaga gcacaaagat aaatgggcaa
    12961 tcgtcagcgg tttgaattta ccaatgttga ttgaagccta cgcatcacgc ttttcaatgg
    13021 aatctgctca tgaaattgca gcacacatca ttgaaacagc aaaagacggt gtaaaagtaa
    13081 aaccggaaga attagaaccg gcagaagcac cgaaagcagc agttgaagac gcacaaccta
    13141 aaggagcact tccagaagga actgtggtcg gagacggaaa aatcaaatat gtattagcac
    13201 gtgttgactc acgtctgtta catggacaag ttgcgacagc ttggacaaaa gcagtgcaac
    13261 caaaccgtat tatcgttgtt tcagatgcag tatcaaagga tgatctgcgt aagagactaa
    13321 tcgaacaagc tgctcctcca ggtgtaaaag caaatgttat tcctattagc aaaatgatcg
    13381 aagtagcaaa agaccctcgt tttggaaata ccaaagcatt gcttttattt gaaaatcctg
    13441 aagacgtatt gacagctgtt gaaggtggcg tagacatcaa agaattaaat gttgggtcaa
    13501 tggcgcattc ggtcggtaaa gtcgttgtaa gtaaagtatt gtctatggga caagaagacg
    13561 ttgaagcgtt tgagaagctt gaacaaaaag gtgtgaaatt cgatgtacgt aaagtaccta
    13621 atgattcacg cgataacatg gatgatattt tgaaaaaagc aaaagctgaa ttagcaaaag
    13681 cgtaataaat aatattaaaa tttataggag gcttatcatg tctattattt caataatttt
    13741 agtcgtatta gttgcctttc tagctggtat ggaaggtatt cttgatgaat ttcaatttca
    13801 tcaaccacta gtggcatgta cattgatcgg tttagttacc ggtaacctcg aagctggtat
    13861 cgtacttggc ggtacgcttc agatgatcgc tttaggttgg gcaaatatcg gtgccgctgt
    13921 agcaccagat gctgcattag catcagtagc atcagcaatt attttagttt taggtggaca
    13981 aggtgttaaa ggtgttcctt ctgcgatcgc gattgccgtt cctttggctg ttgcagggtt
    14041 gttcttaaca atgatcgtac gtacaatcgc tgttccaatc gttcatttga tggatgcggc
    14101 tgctgaagaa ggaaacatcc gtaaggttga aatgtggcac atcattgctg tatgtttaca
    14161 aggggttcgt atcgcggtcc cagcagcagc gttgctattt atcccagcac aaactgtgca
    14221 atcattcctt gaatcaatgc cagcatggtt aacagatggt atggctatcg gtgggggaat
    14281 ggtcgtagct gtaggttacg ctttggtaat caacatgatg gctacaaaag aagtatggcc
    14341 attcttcgtt attggtttcg ttgtagctgc aatttctcaa ttaacattga ttgctctagg
    14401 tgctttaggt gttgcattag ctcttattta cttgaacctt tctaaaatgg gtggttcatc
    14461 aaataatgga ggcggaagca actctggtga ccctttaggc gacattttga atgactatta
    14521 atcttgaagg aggaaaataa aatggcagaa gaaaaaatca aattatcaaa aaaagatcgt
    14581 ttagctgttg cgtggcgctc taccttcatt caaggttcat ggaactacga acgtatgcaa
    14641 aacggtggct gggcattttc tatgattcca gcaatcaaaa aattatacaa aacaaaagaa
    14701 gaacgttctg cagcattaaa acgtcacttg gaattcttta atacacaccc atacattgct
    14761 tctccaattt taggggttac attggctctg gaagaagaac gtgcgaatgg cgcgcctgtt
    14821 gatgatgtag cgattcaagg ggttaaagta ggtatgatgg gtcctttagc tggtgttggt
    14881 gacccagtgt tctggtttac agtacgtcca atgctaggtg cactaggtgc ttcattagct
    14941 atgggaggaa atatccttgg accaatcatc ttcttcttag gttggaacat tattcgttgg
    15001 gcattcatgt ggtatacaca agaatttggc tacaaagctg gttctaaaat tactgatgac
    15061 ctttctggcg gattgttaca agacgtaaca aaaggtgctt caattttagg gatgttcgta
    15121 ttagctgccc ttgtacaaag atgggtatca atcaaattct caccagttgt atcaactgta
    15181 aaattagata aaggtgctta catcgaatgg gataaattgc cttcaggtgg tgaaggaatc
    15241 aagagtgcat ttgaacaagt taataatggc ttagcccttt ctcctgaaaa ggtaacaaca
    15301 ttacaagaca acttagacca attgattcct ggtttagctg cattaggatt aacattgtta
    15361 tgtatgtggt tgcttaagaa aaaggtttct ccaatcgtta tcatccttgg tttattcgta
    15421 gtcggtgttg ttggacacgt catcggactt ttataatcca taaaaatata gctcgggcgt
    15481 tgtcccgagc tttttttata tagtatactg ttcataaaca aatatctgga gggagaaaat
    15541 ggttcaatca atcaatacaa aagtagactt ggtaattgat gctacttcgt ttacgggtct
    15601 gacagattac gggcaaatca tgataggtga taatggtttt gagttttacc atgcgcgaga
    15661 tagacgcaaa tatatccaaa ttccatggga agaagtggat tatgtactag catcagtcat
    15721 gctaaaggga aaatggattc ctcgatatgc cattcaaacg aaaaaaaatg gtacatacac
    15781 attttcttcc aaagaaccta aaaaagtgct tcgtgcgatc agagaatatg tagatgaaga
    15841 tagaatggta caatcattga gtttctttga tgtagtgaaa cgtggtattc gtagtatgtt
    15901 taaacgcaag taaaaaataa agaaaacttc taagaggagg ttttctttat ttttttactt
    15961 tgcagaagaa aatatgaaat agcaaaacca attatttgtc ccctttaaat cccatatgct
    16021 aaaattgatt agcaaagaag gaggttatat tatgagttgg ttatggacat tgattgttgg
    16081 agccgtgatc ggagcaattg caggtgcgat caccagtaaa ggaaattcga tgggctgtgt
    16141 aacgaatatc attgctggac tagtcggttc aagtattgga caagcgattt tcggcgaatg
    16201 gggaccaaaa gctgcaggta tggcacttat tccatctatt ttaggcgcag tgattttggt
    16261 agcagttgtc tcatttttct ttggaaaacg atcataagtt tttacaaaat agtaaactaa
    16321 aaggagttgc agcgagactg tgactccttt ttggctttat ttatttaaaa taaaaagaag
    16381 ttttccggaa atttttattt aactgtcgtg tacgaagaaa agtttgctat aatggaaaaa
    16441 agcgtgcaaa gaaagaggga gtttcattat gatgctacgt gtagaaaaat tgcgcaaaaa
    16501 aatgcaagaa gaaaatttgg attcattttt ggttaccagt ccgtacaatc ttcgttactt
    16561 aacgaacttt acaggtacga caggattagc tgttattact ttggaaaaag cattttttat
    16621 tactgatttt cgttataccg agcaggcggc agcgcaggct caagggtttg aaattattaa
    16681 aaatgtcgga cctatttttg atgaggtagc aaacttagta cgcaaagaag aactgcactc
    16741 attagctttt gaggaaacaa cagtctcatt tttagaatat tcggttttag aagaaatcat
    16801 cgatgcggaa ttagtacctg tttcacagat gatcgaagaa ctcagagaag tgaaagatga
    16861 agaagaaatc gctattatcg aaaaagcatg tcatattgcg gatatggcat atgatcacat
    16921 cctgaaaatg atccaaccgg gcatgactga gattgaagtc gcaaatcaac tagactttta
    16981 tatgcgttct cttggcgctt caggtgtttc ttttgatacg atcgttgcta gcggattgcg
    17041 ttcggctatg ccgcatggtg tagcaagcaa aaaaatcatc gaacaaggtg acttgatcac
    17101 attggacttt ggttgctatt acgaagggta cgtttccgat atgacacgta cattcgctat
    17161 tggtgatcct ggggaaaaat taaaagaaat ttatcaaata gtcttagatg ctcaattagc
    17221 agttattgat gctgcaaaac caggaatgac tggtgtccaa ttagatgcag ttgctagaga
    17281 ttatatttca aaacatggtt acggggaagc atttggacat tctacaggac atgggatcgg
    17341 tttagagatc cacgaaggac caaacgtttc ttctcgtgca gaaaagcaat ttgtaccagg
    17401 aaatgtgatc actgatgaac cagggatcta cttaccagga atcggtggag tacgtattga
    17461 ggatgacttg ttgatcacag aagatggaaa tcgtgtgttg actcgttcac caaaagaatt
    17521 gatcatctta tagtttcatc aatagaagaa gtaagcgtaa aaatcagtgt tcacagactt
    17581 tgttatagaa aatcactcga tttaatgata cactatagaa gaacttttaa gctgtaagag
    17641 ataataaagt tgggtgtttt ctccaattca tagcagtttg cagctgatcg gcggcagttt
    17701 tcttaccacg tgatcactat gccgcatcat agagaattcg gatgtatctc gtgcttaggc
    17761 aaagggagga ataaataaat ggcagatgaa aaaaatttga ttttaaacgc aaaccaagat
    17821 ttaggagaga tcgtgatcgc tcctgaagtc atcgaagtaa ttatcgggat tgcagcttct
    17881 aaagtagaag gcgtatacgg aatgcgcgga acttttgcca gcaatgtcac tgaattatta
    17941 ggacgtgcag ctcacggaaa aggtgtttac ttagcaaacg aggaagatgg cttgaaagta
    18001 gatctttact gttatctaga atatggtgtt tcagttccta aagtagcaat ggaaatgcag
    18061 gaacgggtca gccagcaagt ccttttcatg acggatatcg atttatctga agtcaatatc
    18121 catgtagtag ctgttgttcc tgaaaaatta gaacaaccaa caatcgaaga actttttgaa
    18181 gacgaagagg aagagaatga ataaagaatt atcaagacac gagattcggg aaatggcatt
    18241 gcaggcttta tttccattag acttcaatgc agacctaaca aaagaagatg cgattttcaa
    18301 cgcaatcgaa ttagaccacc gcgacatgat caacgaagat gaatcagagt ttgttcctgt
    18361 ttatcttgat acgctagttg gcggtgtctg tgcaaaaaaa gatgaattgg ataaagtgat
    18421 tgaaaaacat ttaaaaaaca attggcacat caatcggatc tcaaaaatgg atttggtgat
    18481 cttgcgtatc gcgatttttg aaatgactta tgtgacagat gtaccagcgg ctgttgcttt
    18541 aaatgaagca atcgagttgg caaaaacatt tagtgatgac cgttcacgta aattcgtcaa
    18601 cggtgtttta tccaatgtat tgaaagaatt agaagctggg gcgtaagtcc tagctttttt
    18661 ctatgaatca tcataaacct gttataaatc gggaaaattc tatggtagaa tgaaaacaca
    18721 aaaaatatct gggagtggtt agatggcaga attgattaac ggaaaagaat tagctgaaaa
    18781 aatgcaggca gagattgctg aaaaaatcca aaaattgaaa gataatggta tccatcccgg
    18841 attagtcgtt ttattggtag gggaaaatcc agccagccaa atctatgtaa gaaataaaga
    18901 acgtgtagca aaagaaatcg gaatccattc attggtagag cgttatcctg agacgatttc
    18961 tgaagacgaa ctacttgcag aaattgaaaa atacaatcaa gatcctagtt ttcatggtat
    19021 attggtccaa ttaccattgc caaagcatat agatgaagaa aagatcctgt tagctatccg
    19081 ccctgaaaaa gacgtagacg gttttcatcc gatgaattta ggcagactcc ttgcgggtaa
    19141 accggacaag attccttgta ctccatacgg catcatgaaa atgtttgaag cttattctat
    19201 cgatccggcg ggtaaacgag cagtagttat cggacgaagc aatatcgtcg gtaaaccgat
    19261 ggctcagcta ctcttgatgg cagatgcaac tgttactgtt gctcattcta aaaccgaaaa
    19321 tctggctgaa ctggcacaag aagcagatat tttagtcgta gcaataggac gtggacattt
    19381 tgtcacaaaa gaattcgtaa aaccaggtgc tgtagtgata gatgtaggga tgaatcgtga
    19441 tgctaacggt aagctgatcg gtgatgtgaa atttgatgaa gtggagccta ttgcaggctg
    19501 gatcactcct gtgccaaagg gtgtagggcc aatgactatc acgatgctga tgtatcaaac
    19561 cgtaaaatct gccgaagctt ttggagcagg tgaatagatg gcacaagaat atttaactgt
    19621 cactacatta acgaagtatt tgaaaagaaa atttgatgcc gatccttatt tagaacgcgt
    19681 atatttaaca ggcgaaatat cgaatttccg tttacgtgcc aatgcccatc aatatttcag
    19741 cttaaaagat gaccatgcaa aaatatctgc tattatgttc aaaggagcat tccaaaaatt
    19801 acgattccag ccaaaagaag gaatgaaagt ccttgtcgtt gggcgtattt cattatatga
    19861 agcgggaggt tcttatcaaa tctatgtaga gcacatggag cctgatggaa taggtgcttt
    19921 atatcaagaa ttggaagaac gaaaagaaaa gctgatgaag gaaggccttt ttcaagggcc
    19981 taaaaaaatg ctgccacgtt tcccaaagcg aatcgcggtc cttaccagtc caagcggagc
    20041 ggttatccgc gatatcatca cgacggtgaa aagaagatat ccaattgtcc aattggtttt
    20101 gttcccaaca gttgtacaag gaaaccaagc agcggacaat gttgtcagga atatccagag
    20161 agtagaagag atgggcaatt tcgatacaat gatcatcggt cgtgggggtg gttcgattga
    20221 ggatttatgg ccgtttaatg aagagcgggt tgctcgggcg atttatcaag ctaatacgcc
    20281 ggtgatttca tctgtaggac atgaaacaga taccacgatc gctgatttag tagcagatgt
    20341 tcgggctgcc actccaacag cagctgcaga attagctgtt cctgttttgt cagaagagtt
    20401 gatgcgcatt caagaacggc aagctagatt gcagcaggcg tttgtacgcc agatccagcg
    20461 caaacaagaa cggttcgagc gtgctcgacg ttcctatgta tttcggcagc ctgaaagatt
    20521 gtatgaaggt cagaccatca aattagacca gttgaaacag cgattatatc aagctacaca
    20581 gcaaatttat catgcgaaag aaaaacaaac gatcgcatta agccaccaat tggaacaagt
    20641 ggctccgctt tatcgagtta aagcagctcg ccaagaaact gcttatctag aaaaacgctt
    20701 agaagagaaa atccaacaat atatgaaaca acagcagcag cgttttgctc aagctgttca
    20761 atctcttgat ttgttgagtc cattgaaaat catgggaaga ggctacagct atacgacgtt
    20821 aaatgatcaa gtagtaaaaa gtgtcaccga gattcatcaa ggggatttgc tgacgatcca
    20881 ttatcaagat ggaatcgtta aaggacaagt acaagagata gacaaaggag aataaaaata
    20941 tggaaaatcc aacttttgaa gagtcactgc aagaattaga aaaaattgtc atgcagttag
    21001 aacaaggaga cgttccatta gaatcagctc tagattcgtt taaacgagga atggaattaa
    21061 gcaaacattg ccaagataca ttagctaaag cagaagagac cttgacgaaa atgatgacag
    21121 aatcaaatga agaagttgat tttgacggaa acgaggaaag ctgagatgag attaactgac
    21181 ttttcagcta ttcatctacc tgctgtagaa aaagaaatct tgtctttttt agatgagcat
    21241 acaacggata gaggactcta tgatgcgatg agttattcag tgaaagcagg cgggaaaaga
    21301 atacgtccct tattactttt aacggccgtt gcttcctttg atgagcctat tgatgttccc
    21361 gtctatcaag tagcagctgc tttagaaatg gtgcatacgt attccttgat ccacgatgat
    21421 ttgcctgcga tggacaatga tgatcttcgt agaggaaaac ctaccaatca taaagtattc
    21481 ggcgaagcat tagctatatt ggccggagat ggtttgctta caggagcatt ccagctaatc
    21541 agtatggcac acttaggtaa ttctcctaag cttctgctgc ttcagcaatt agctgtttgt
    21601 gcgggatcac aaggtatggt tgctgggcaa gctgcagata tcgagggcga atcgaaaaag
    21661 ctttctttag aagaattagc atttattcat gaaagaaaga cagggcattt gattcgttat
    21721 gccttgctgg ctggtggcat attagcgaag caacctgaag aaatcttatt gctacttcaa
    21781 cgattagccg aacatttagg tttagctttt cagataagag atgatctact cgatgtgatt
    21841 ggtacaacga aaacattagg gaaaacagct ggtaaagatg aacgtatgga aaaaaatact
    21901 tacccgcgtt tattaggcct agaaaagaca cgtgaggcgt tggagattga gctgttatcc
    21961 gccaacaaga tcatagacaa attagaagag gacgtttttt cttttgatgg cgggctgatg
    22021 cgccagatga tcaagcaatt tgatgtggag aaatgaggga aagaatggaa aaagaacgcg
    22081 tagatgtatt agcggtcaaa caaggattat ttgaaacaag agaacaagcc aaacgttcag
    22141 tgatggctgg tttgatttac aacgaaaaaa acgagcggtt tgataaaccg ggagaaaaaa
    22201 ttccagtgag tagtgaatta agagtcaaag gtaaaaaact tccttatgtt tctcgagggg
    22261 gattaaaatt agaaaaagca ctcaaagcat ttgatctatc tgttgaagga aaaacattgt
    22321 tagatattgg tgcatccact ggcggattta ctgatgcagc tttgcaaaat ggcgcaaaaa
    22381 tgagttatgc tttagacgtt ggctacaatc agctagcttg gaaaataaga caagatccta
    22441 gagtcgtcgt aatggaaaga gtgaattttc gttatgcaaa acccgaagac tttaccttag
    22501 gaattccaga agttgctgtt atcgatgttt cttttatttc actaaaactg atgctgcctc
    22561 cattacatgc tattttaaaa gaagagggag aagtgattgc tttaatcaaa ccccaattcg
    22621 aagcaggcag agaagctgta gggaaaaacg gaattgttcg tgaccctcag acccatcaaa
    22681 aagttttaga agacattttg tcatttgcgg cacaaggtgg atatgatgtg ctggaattaa
    22741 gttattctcc gatcacgggg ggagaaggaa atatcgagtt cttggctcac ttgaggaaag
    22801 tacccgaaag cggaacaatc aattcagcta ttaatatggc agaagtggtt tcaaatgcac
    22861 acgaacagtt tgaccataaa taaattgtaa ctgtgaaaat caagtgagaa gtatgcggat
    22921 tcttctttat tataccaagg aaataagcta tcattgatta agaagaatat gctaaggagc
    22981 gaagacgtaa tgagaaaaaa agatcggcat cgtttgatta ctcgattgtt gagtgagcaa
    23041 gatatccgaa agcaggaaga atttgttgaa atcctgaaaa gcaaaggaat atctgttact
    23101 caagctacga tttccagaga tataaaggaa ctgaaactga tcaaggtacc ggcttcagat
    23161 ggcggctacc gttatagcat accagctgaa acaaacgaag atgtaaatac taaattggaa
    23221 aaactattaa aagacgcttt cgtggcagtt gaccaaatgg aaaaatttgt tattttgaaa
    23281 acattgccgg gaaatgcttc ggcagcagca aatttgattg ataaacgtta caagaaagaa
    23341 ttattttcga ttatcaatga tgacgataat gttttgatga ttacacgaac tgaagaccag
    23401 gcagttttat tgaagaaaga cttcctgcat tacctttaag ttgaggagcg aatgtgatgc
    23461 tacaagaaat cagtattact aactttgcga ttattccgga attgcggctt tcttttcacg
    23521 aaggaatgac cgcgctgacc ggagagactg gtgcgggtaa atccattatt atcgatgctt
    23581 taggtctgtt agcaggagga agaggttcaa gcgattatat acgtcaagga gcagaaaaat
    23641 gtgtgcttga agggttgttt gaactaccaa aacaagaagg attctctgaa ctgatggttg
    23701 aattagggat cgaaacagat gaggacaatc tgattgttcg acgggatatg tccttaactg
    23761 gaaaaaatgt ttgtcgagtg aatggtcata ttatcacatt agctaatctg agaagaatcg
    23821 gcagttattt agtagatatc caaggacaaa acgaacatca ggaactgctc cagcctgaat
    23881 cacatcttgc tttattggat cgttttggag atgtagcttt ccaacagaag aaaaagagtt
    23941 atcagcaaga atatatctct tatcgtgagc tggaaaaaag ggtacgaaaa gtgcagcaaa
    24001 atgaaaaatc ctatgttcag agaatagata tgctccattt ccagcaagaa gaaatagctg
    24061 ctgctgattt gcaagtcgga gaagaagaga aattaaaaga agaaagagaa aaacttagca
    24121 attatcaaaa aattgctgat ggattagctg caggttacgg agcactgacg aataatgaac
    24181 aaaacagtgt agatggtgtt ggcttggccg tttcagaact gcaagggatc gcccatctag
    24241 atgtggagta tgaagcaatt tatgaaaata tccaaagtgc ttactatttg ctgcaagatg
    24301 caatcggcga tatgagtcgt cagattgatt tattggagtt agatgagagt cgtttagaag
    24361 aagttacgca acgtttagaa ttaatccgac agttgaaacg taaatacggc gaatccattg
    24421 agtcaatatt ggcttactat gacgaaatca ccgaagagct agcttcttct gatttttcag
    24481 aaggtcaatt ggacaaaatg aaagaggaac tagagcaaaa agagcttcta cttcagcaac
    24541 aagccgcaga tcttcatgaa gcgcgtaaaa aaatcgcaaa agaattggaa aaatccattc
    24601 tgcatgaatt gaaaagtctg tatatggaaa acactgagtt tgaggtacgt ttcttaaaag
    24661 aagaaaatag acaattgaat catgatggat ttgaccaaat cgaattttac attacgacca
    24721 atccaggaga gccactgaag cctttggtga aagtcgcttc tggtggcgaa ctgtctcgga
    24781 tgttgcttgc tttgaaaact attttttctt ctgagcaagg tgtgacaagt attatttttg
    24841 atgaagtgga tacaggtgtg agcggaagag ttgcacaggc catcgcagat aaaatttctc
    24901 aaatatcgaa atactctcaa gttttgtgca tcactcatct gcctcaagtt gctgcagtcg
    24961 ccgactatca atattatatt gtgaaagcag tgattgatgg acgtacgcaa acttctgttt
    25021 cagagttgaa aacaaaagaa agagaagaag aaattgcacg tatgctggct ggaagtgaga
    25081 ttacaaaact cacactcgaa catgcaaaag aactattgaa actcgcaaaa aggacctgag
    25141 ccacctcgca gtggttcagg tccttttcag cagctgatag ctatatttta tttttacatg
    25201 acactcatta cagcagtcaa tacaacagat ccaatcgtta aaatcagcat tgtccaaatg
    25261 acaaattttg ttactttagc aaaagtactt gtttgttttt tttcacgcat tgttttcaac
    25321 ctcgtcatta attagttgat tcaagtgtac aatctttttt ccatttccac aagagggctt
    25381 ttttataaaa acttcccttt ttttaaataa taggtagtta cgatacaaat aaataatgtt
    25441 aatagaacta gccatataaa ggcatcatcc ctttcagcaa taggtaactt aatgttcatt
    25501 ccatagatac tgccgacgat tgtcggtata gttaaaataa tcgttaaaga agttaaaatt
    25561 ttcataatat tattcagatt attcgatacg attgctgaaa aagtctgatt catttgttct
    25621 actagtttta attgaatgcg agaagtagtc attgcctgct ctgtttccac caatacatca
    25681 cgtaaatgat ttttcaaagc atgattttct tggaaaagtt tattttgcgc tagttctttc
    25741 aatgcatcta aattagcaga agtcgcttct ctgaaataaa cgaggctttt ttggatatcc
    25801 atgatttgat aaagttgttt gttttcagtg gatacttgaa gtttggcttc caaagtatcg
    25861 cattgagatt gaagcgtagc taaataactg ttgtaagaga gtgtgacctg ccaaatgaat
    25921 tgaaagaaga tattgagatt aggcgaaata tcactttttg gaaaggtctg gctaaagact
    25981 ttctttaaaa acagtggttc atgattcgta attgtgataa gcttcttttt gggcgtcaaa
    26041 ataatagaga gcgggtatgt atccacttga aaataaccgc taggacttgt tttagcaaaa
    26101 ggatattgta acaataataa cgctggcttt gtcaactttg attgattgaa ttcttctgta
    26161 cgggaatttt ctgcatcgtc caatatggaa gtgatgtaat ctgacggaat ttcaaattga
    26221 tgtgtcagct gatgaatctc ttcttgatta ggacgttcta cacaatacca gatcgtttgg
    26281 tcagtttcct ttccggtatt acgcagtttc tcattatcta atgtaaaata tttgatcatg
    26341 attttcctcc tgttgcctgt ttcttttatt ataacggata gggaattgga atacatggaa
    26401 tgtgtttgta agatatagaa tataactgca taaatttaaa taaaatgaaa cagttgtgta
    26461 aagtatatac agtatacttg aagcaacgat aaaaaaggag tttacgttca tgggcacatt
    26521 cacaccactt atcttgtttt ggtatattta cccggtaatc gttttgtttg gttgccaatt
    26581 tcttgtaaaa ttattttcgt taaatcgtcg ttttaaaata aaagcccctg atttggcgat
    26641 tccttttttg tggggcggta tgcatgcatt gtctagaaat acagggacaa tttctatctt
    26701 accgtttttc tttatttctg ttttgctaat gggaatttgt attgctgttt tccaagcata
    26761 ttattatgaa gaaattattt atccgcgtta tttcaagatg acttggcgtt tggtttttct
    26821 gttgacaatc gtcttgtacg ctgtcttgat cattgtgaac atactttctt acctatgaaa
    26881 aaaatagaaa agctgtaaaa agtcttttaa aaggcttttt gcagcttgat ttttaaaaaa
    26941 atgtttatga ataaaaaaag ttgttcccgg atttcccaga aaaggtggta gaaagtgggg
    27001 gattgtggta gactaatttt agttagtgga ggaatggggg gctttcaatg ttgatgggtg
    27061 aattccaaca taatattgat gcaaaaggac gtttaatcgt gccctcaaaa cttcgtgaag
    27121 aacttggcga aaagtttgtt ctgacacgcg gattggatgg ctgtcttttc ggctatccga
    27181 tgagcgaatg ggaaaatctg gaagctaaac tgaacgaaat gccgttggct aaaaaagacg
    27241 cacgtacttt tgtgcgattc ttctattcgg ctgccactga atgcgaactt gataaacaag
    27301 gacgaatcaa tatacccagc acgttaagaa attatgcagc tttgacaaaa ggttgcgtag
    27361 tcatcggtgt atctaaccga attgaaattt gggatgagac acgatggcaa gatttttctg
    27421 cagcagcaga agagaacttt gacgaaattg ctgaaagtat gattgatttc ggactataag
    27481 aataggaaga ggctgactaa tgactgaaac atttcaacac tatacagtca tgttgaaaga
    27541 aacggtagat ggattgaaca tcaaaccgga tggcgtttat gtagactgta cactcggcgg
    27601 tgccggacat agtgaatatt tattaagcca actaagttct aaagggcatc tgtatgcgtt
    27661 tgaccaagat caaaaagcaa tcgatcacgc caagctgcgt ttagcttctt atgtcgaaca
    27721 aggtatggtc acatttatca aagcgaactt cagagaatta gaagaacgct tgagtgagca
    27781 tgtagataag gtagacggca ttttgtatga tctaggggtt tcctctcctc agctagatga
    27841 agcagaacgt ggatttagtt atcatcaaga tgcaccgttg gatatgcgca tggatcagtc
    27901 agctccttta tccgcttatc acgtaattaa tgaatattcc taccatgaat tagtaaagat
    27961 tttcttccgc tatggcgagg agaaattttc caaacaaatt gctcgtgaaa tcgaacgagt
    28021 acgaaaaaca aaaccaatcc aaacaactgg tgagcttgtc gaaatcatta aacaagtcat
    28081 cccagctccc gcaagaagaa aaggcgggca tcctgcaaaa agaatttttc aagctgtgcg
    28141 gattgcagta aatgacgaac tcggtgccga agaggcctct ttagaacagg caatccggtt
    28201 attgaaagtc aatggaagaa tcagcgtaat cacattccat tcattggaag acagaattgt
    28261 caaaagtatg tttaaagaat atagtacggt acaagatcta ccaccgggac ttcctgtcat
    28321 acctgacgaa ttccaaccag aactgaaagt cattactcgt aaaccaatcc ttccttcaga
    28381 ggaagagcta acagaaaaca atcgatcacg cagtgcaaag ttacgtattg cagaaaaaat
    28441 aagaatgaaa gaggagtaga atgatggcag aattaaagaa aatgcaagat tacgaatatg
    28501 atttacctga attggtggat cagccggaac aacaaatgcc tgattcttct tcgaatcaag
    28561 aaatcattct tccacaatca ccggcaaaac gattaaagca gatttctgct ttggaaaaaa
    28621 ttgctgtggc aagcgtggcg attgctattg ttgctttatg tgttttaaca gttatgctgc
    28681 gcacaaatat cagcggagct gaaaaagaaa tcacgtcaat acaaacagaa atcaatgata
    28741 aaaagcaaga aaaaacgagt ttagaacaag aaaaaaacga actttctcgt actgaacgga
    28801 ttaaacaaat cgcagaagaa aaaggattgt ctattaatga cgacaattta aggaaagtga
    28861 agtaaatgag tcttaaaaat aaaattcggc ggtttatgga aaagaaaaat ctaaatccga
    28921 tgaacaatcg caaaaaagta ggtatcattt tatttgctac gagtattgga ttgttctttt
    28981 tatttgccgt ccgtttctcg tatatcgtta ttggaggtca tgttgctggt acctcattag
    29041 ctgaaaaaac aaaacagttg taccaaggaa gtgaagtagt aaaagcaaaa agaggaacga
    29101 tttatgatcg taacggtgtg gcgctcgcag aagatgcctc ctcttattca attaaagcga
    29161 tactctcaaa aacatatacc tctggggata aaaaactgta tgttgaagaa aagaattttg
    29221 ataaaatagc agaaatcctg cataagaatc tttctataga caaaaaagat gcactgaata
    29281 ttttagagga cggcgcaaaa aaagagctgt atcaagttga atttggtagc tatgggaaaa
    29341 atatctccca agaaaccaag caaaacatcg aagcggacat gaaaaaagaa ggtgtcgctg
    29401 gtctttattt tgtcgaccat caggctcgga tgtatccaaa tggcgtcttt tcttctcatt
    29461 ttattggata cgcagttcca gacaaagatg agaatggctt agttggtaaa ctaggtttgg
    29521 agtctgctta caatgatatc ttgagcggta aagatggtaa gattatttat caaaaagata
    29581 attttcaaaa tcctctgcca ggtacagtgg cagaagaaga aaaagctgtc gacggacaag
    29641 atatctacac gactttagac agcagactac aaagttattt agaaacactg atggatcaag
    29701 taaatgaaga atatcagcca gaagaattga cagcagtcct gatgaaagca aaaaccggag
    29761 aaatcttggc aatgggacaa cgacctactt tcaatcctga aacaatggaa ggattgactg
    29821 gaaaagatgc tatctggcgt aatttcttgg tacaagatag ttatgaacca ggatcaacca
    29881 tgaaagtttt cactactgca gctgctatcg aagaaggaga attcaacgag aatgaaacct
    29941 tccaatctgg aaaaatccaa gtagaagatg ctacgatcaa tgaccatgac tttggtgaaa
    30001 aaggtgtgct gaccatgcgt caagctcttt catggtcaag taacgtcggg atggtcatac
    30061 tggaacagcg attaggcgga cgatggtata actatttaca aaaactggga tttggacaaa
    30121 gtactcattc tggattagat gatgaagtaa acggtgcttt acctacatta aatattgtcg
    30181 accgagcaat gagtgcttat ggacaagcgg taggtgtgac gaatttccaa atgatgaaag
    30241 ctttcacttc tattgcgaat aacggtacaa tgattcaacc tcgatatatc agcaaagtgg
    30301 tggatcccca aactggagaa gagcgaacaa ctcaaacaga agttctaggg caaccttttt
    30361 caaaagaaac aacagaaaaa gtccgtgaat atatgagaga tgttgtcgaa agcgaaaact
    30421 acggtagtgc ttatggtgta tatagtgtac ctggatacaa tgtatcggca aaaaacggaa
    30481 cagcccaaat cgcttctgat acagggggat atcaaacagg agatacagcc tacctgtatt
    30541 cgattgtgga gatggttcct tctgaagatc cagactatgt cttgtatcta acaatgaaac
    30601 accctaagac atatgatcga atggcattgg ctaaaatcgc aaatccattg atgaaacggg
    30661 caatggattt taaagaaact gaagaagatt cagatacaga aacaaaaaca gaaaaagtat
    30721 ccgtagccga ttatcgtaat cttgaagcgg acgttgcagc agctgatgct caaaaaagtg
    30781 gactgcaacc tgtagtgatc ggtaatggta aaaaagtaca aaaacaatct actgccaacg
    30841 gtgatcagtt gatctctgga gaaaaactga tcctatatac tggtggtgat aagctgatgc
    30901 ctgacgtaac aggatggtca aaagcagata tcatgaagct ggggaaaatc ctcggtattg
    30961 aagttagttt tgatggagat ggttattgtg tcaaacaaga acttgctcca tatgaaaaaa
    31021 tcactgaaga caaacttagt tttactttag aagaataaga agagaaatag gagagaaaag
    31081 agcatggaat ggacacaagc attgattccc attgtcagca gttgtgcaat gacaattgcc
    31141 gcaatgccgc tatttattgg ttattttcaa atgaaaaagc aaggtcaggc gattcgtgag
    31201 gagggaccaa agtggcacaa tagcaaagcc ggaactccta caatgggcgg cttagtgttt
    31261 ctgatcggct ctattttgac aggaatttgg gttggagctt ggcaaaaaca gctaactcct
    31321 actcttttca tcttgctttt tgtattagcg ttatacggag taatcggctt tcttgatgat
    31381 tttataaaaa tattcaagaa aagaaacatg ggactgaact cgaagcagaa attgattgga
    31441 cagattctag ggggaatcat cttttatctt gtttaccgtt ctgaaggtta tccaggtgta
    31501 ttgaatttct tcggactaga tctaccttta ggaatcattt atggtatttt cgctctcttc
    31561 tggcttgttg gattttcaaa tgcagtaaat ctgacagatg gcatcgatgg attagttgct
    31621 ggcttaggaa gtatctcttt tgcagcttac ggtctaattg cttggcatca gcatcagtat
    31681 gatgtattaa tcatttgctt aagtgtttta ggaggtttac tgggtttctt cgtctacaac
    31741 agaaaacctg ccaagatttt tatgggagat gtcggttcat tagcgttggg cggactttta
    31801 gcggcaattt ctataatgct aaaccaagaa tggacattgc ttttgattgg tttgatctat
    31861 gttatggaaa cagcaagtgt gatgcttcaa gttacttcct ttaagcttac aggaaaacga
    31921 atttttaaaa tgtctccaat tcatcatcac tttgaaatgt gcggatggtc ggagtggaag
    31981 attgatacag ttttttggtt gatcagcatt gtgacttcgt tgatcacatt atggatcgtt
    32041 tggtaggaga tgacgggaaa tgaaaaaaat atcaacatat gaaaataaga aagtgcttgt
    32101 cttaggactc gcaaaaagcg gagtaagtgc ggccaaatta ttacatgaat tgggcgcact
    32161 tgtcaccgtt aatgatggaa aaccttttga cgaaaatccg gaagcacaag agctgctttc
    32221 tttagggata aaagtaataa caggtagtca tccaatcgaa ttgttggatg aagaattttc
    32281 attgatggtc aaaaatccag ggattcccta cagtcatcct ttagtagcaa aagcccaaga
    32341 aatgggtatt ccggtaatta cggaagttga gctagcttac gaagtagcgg aatgcccaat
    32401 tattgggatt acaggaacga acggaaaaac aacgacaaca acgatgacag gactactact
    32461 gaatgcagga gcagatcaag gaattgcacg tttagcgggt aatattgggt atccagcaag
    32521 tggtgtagcg caagaagcaa aatcagaaga taaaattgtg atggaattgt ctagcttcca
    32581 actcatggga atcacagatt tccgccctca tatagcggtg attaccaata tctatgaagc
    32641 acatattgat tatcatggta caagaaaaga atatgtcaaa gctaaatgga accttcaaaa
    32701 aaacatgaca gaaaaagatt atctgatcct taactggaat cagtcagaac tacaagaatt
    32761 agcgcaaaga acaaaagcac gcgtactgcc tttctcgaca aaagaagtgt tggaagacgg
    32821 tgtgtatgct gacgattact caatatacta taaaaaagaa aaaataatgg aaatatctga
    32881 acttggcgta ccaggtaagc ataatgtaga aaatgcacta gctgctattt ctgtagcaaa
    32941 attgtatggt atatcaaatg aagctatacg agaaacgttg cattttttcc atggcgtacc
    33001 acatcgtacg caatatgtag gagaaatcca aggcagaaaa ttttataatg attcaaaagc
    33061 aaccaatatt ttagcgacta aaatggctct aagtggattt gaaacgtcta aggtcgtgtt
    33121 attggctggc ggattagaca gaggaaatac atttgatgaa ttaattcctt cattaaaagg
    33181 aatcaaagca atggtcgtat ttggccaaac aaaagaaaag ttgatggatg ctggaaaaaa
    33241 agcaggcatc gagacaatag taacagccga ttctgtagaa caagcagttc ctttagcatt
    33301 agaaaacagt acagatggtg atgtagtttt attatcacca gcgaatgcca gctgggatca
    33361 atacccaaat tttgaaacta gaggaaacag attcatggaa gctgtgaacc gattaaaata
    33421 gaaaaagtag aagcgagcga taaaaaatga aaattttggt tacaggcgga ggaacagggg
    33481 gacatattta tccagctctt gcctttgtca attatgtaaa gactcaagaa ccgaatgctg
    33541 aattcatgta tgtgggagca aaacgaggtc tagaaaacaa aatcgtccct gatacaggaa
    33601 tgcctttcca taccttggag attcaaggat ttaaacgaaa aatttccatg cataacatca
    33661 agacggtccg attgtttctt aagagtatcc gtcaagcaaa aaaaatcctc agtgattttc
    33721 aaccagatat cgttatcggt actggaggat atgtttccgg agcggtagtg tacgctgcag
    33781 ccaaaatggg tattccaaca gttatccatg aacaaaacag tgtaccaggt gttaccaata
    33841 aatttttaag ccgatatgtg gatcggatcg ctctttcatt taaagatgcg gcttcctttt
    33901 ttcctgaaaa caaagcagtt cttgtaggta atcctcgagc acaagaagta gcagatacta
    33961 aaaaatcaga tattttgaaa acatttgatt tggatccaga aaagaaaaca gtcttgattt
    34021 ttggcggtag tcaaggagca cttaaaatca atcaagcagt atcagttttt ttaagtgaat
    34081 atattccttc ttcttaccaa gttttgtatg cttcaggaga tagatattat gaagagatcc
    34141 taaataaaat aaatcctgaa atacaaaata ttagtgtcca gccttatatc aaaaatatgg
    34201 cagaggtcat ggcttccagt gatctgctgg tggggcgtgc tggtgcaact tcgatcgcag
    34261 aattcacagc tctggggtta ccggcagtgt tggttcccag cccatatgtt acgaatgatc
    34321 atcagactaa aaatgcaatg agtcttgttc acgcaggagc agcaaaaatg attgcagata
    34381 atgaactaac aggtgaatca ctaagtcaga cagtcaacga aatcatgggt gatgaagaat
    34441 tgcaaaagca aatgtgccga gcttctaaag aacaaggaat ccctgatgct tcaaaacgtc
    34501 tttatgactt agtaaaacaa ataatcaaaa aataaaggcg gtggtcttag tgagtaagaa
    34561 aaaagaacca aaaaaattgt ctcatccgtc ttctgatgaa aacaatttga ctccttggca
    34621 aaaggctaac cgaaaatatc tggcagaaca agagatgaaa aaccaagagc caaaaaaaga
    34681 agacataaca ttagatacac ccctttcaga ttctgaagaa gaaaaaacgg ttcaagagga
    34741 acagcaagaa gaaagccagt ccgagaaaaa aatcgaattt tttgaagaga tcaagcaaaa
    34801 gaaaaacggt ggaccatata acggttcttt tttgaaccgg ctgcccaatt taaaaagtca
    34861 acggcacaaa gtactatttc gtcggttagc tttgatcatc actgtattgg gtataccgct
    34921 gatttttttg atctattatg tttctcctta tagcaaatta caagcagttg cagtatcagg
    34981 caataagaca gtcaacagcc aagaaatcat ctctgataca aaattgtcat taggagaaaa
    35041 tgtttgggga cagtattttc atcgatctac ttatatcgat cgccttaaaa aagcacagcc
    35101 aagaatcgaa acagcaaata tacatttcaa agggatgaat gaattcgatt tggatgtaac
    35161 agaatataaa gagattgctt tgatcgcaaa aaacaatcaa tactacccag tgattgaaaa
    35221 tggaacagta ttagatgaaa aagtagcaaa tcctacaaaa aatctcccta tcttggaaga
    35281 gttcaaagac tctgcaaaaa tcaaagaatt aacaaagcaa tacaatcagt tatcatcaga
    35341 attacaaaaa gcaatttctg aaatcaagta cacgccaaga gcaagcaata aaaatctgat
    35401 ccaattgaat atgaatgacg gcaatcaagt tattgtaaat atcaataatc ttgcgaacca
    35461 aatgaaatat tattcgcaag tagccaaaga tatggatgaa aaaggtgtga tcgacatgga
    35521 agttggtatc ttctcgcatc cttacggtag cactcaagaa acggaagaat cgggccaatc
    35581 ggaagaaaca actgaaagtg aagaaaatgc ggatcaaaca aacgaatcaa gtgaagaggc
    35641 agaaacgcaa aaagcacaaa cacctggatc gtctagcaca aacggatcat ctgattcaaa
    35701 tgattcaagt gaaacaagtc aaatagaaga taatccaagt acaactccgt caagtttaaa
    35761 tgaaaattga taaaacaact ttaaaaagta agaatgtact ttctaaaaac cagtaattta
    35821 tggtagaatt ggaagcagtg aatatatcta ttaataagta tattttaaaa cggacgctag
    35881 ttgttcgctt gtatatggaa tagtaagtag gagggaatcc cattcatggc aaaaacagga
    35941 atgtatgtag gccttgatat tggaacgaca tctgttaaag ttgtcgtggc tgaatatatc
    36001 gacagccaaa tgaatattat tggagtagga aacgcaaaat cagaagggat taaccgaggc
    36061 attatcgttg atatcgataa aacggtccaa gcgatacaac gagcagtacg acaagcagaa
    36121 gaaaaagcag gtatccagat caaaggcgta agtgtcggtt tgccagcaaa catgcttgaa
    36181 gtagaaaatt gccagggaat gattgcagtt aacggagatt caaaggaaat caccgatgaa
    36241 gatgtcagaa atgtggcttc agctgccctt gttcgttcaa tacctcctga acgtcaaatc
    36301 gtgtcaatat tgccacaaga ttttactgtc gatgggttcg agggaatcaa agatcctcgt
    36361 ggaatgatcg gcgtaagatt agaaatgtac ggtctgttgt ttacaggacc taaaacgatc
    36421 atccataaca tacgtaaatg cgtggaaaat gccggattaa tcgtcaacga aatggtgatt
    36481 actcctttag cgctgactga atcaattctt tctgacggag aaaaagattt tggtacgatc
    36541 gtcattgata tgggtggcgg ccaaacaaca acggctgtta tgcatgataa gcaattgaag
    36601 tttaccaatc ttgaccaaga aggtggcgaa tttgtcacta aagatatctc tattgtatta
    36661 aatacgtcat ttaacaatgc agaagcgttg aaaataaact acggggatgc atacccagaa
    36721 cggacatcac cagatgaaga atttccagta gatgtgattg gtcagtcaga accagtgaaa
    36781 gtcgatgaac gctacttgtc agaaattatt tcagctcgaa tggaacagat tttcaacaaa
    36841 gcaaaagatg cattggacca aatcgaagca ttagagttgc caggtggcgt cgtattgaca
    36901 ggcggagctg ctagtcttcc tggtgttgtg gacttggcac aagaaatctt cggcgtaaat
    36961 gtaaaattgt atgttccaaa tcacatgggg ctgagaaacc cggtatttac aaatgtaata
    37021 agtatcgtgg attattcagc aaacttgagt gaagtgtatc aattggcaaa aattgctgtt
    37081 acgggtgaaa cctcagcagc tcgtcaaatg gtagttgaac aagaaacaac aaacacatat
    37141 gagagctatg aagcacctga agaaacaatc tacgatgagc cggaaccaaa agagtccgga
    37201 gaaaatgtaa aaaataaaat caagggtttc tttacaaata tttttgatta attcatcctg
    37261 ggaggaataa gatatggaat tctcaattga taataatatc aatgacggcg cagtaatcaa
    37321 agtcatcgga gtcggtggtg gcggtggaaa tgctgtaaac cgaatgattg aagaaaacgt
    37381 taaaggtgtt gaattcatta cagccaacac agacgtgcaa gcattaaaaa attcaaaagc
    37441 tgaaacagtt atccaattag gccccaaata tactcgtggt ttaggtgctg gttctcagcc
    37501 agaagttgga caaaaagcgg cagaagaaag tgaacaatcc ttacgcgaag ctttagacgg
    37561 tgcagatatg attttcatca ctgcaggaat gggtggagga accggtacag gtgctgcacc
    37621 aatcgttgca ggtatcgcga aagaattggg cgcattgact gttggtgtcg tgacacgtcc
    37681 attcactttt gaaggaccga aacgtggccg ttttgctgca gaaggtatcg ctcgtttgaa
    37741 agaaaatgtg gatacattat taattatctc aaataaccgt ttattagaag ttgtagataa
    37801 aaagacaccg atgcttgaag ctttccgtga agcagataat gttttacgac aaggtgtaca
    37861 aggtatctct gatttgatca cagcgccagg ttacgttaac cttgactttg ctgacgtgaa
    37921 aacagtgatg gaaaaccaag gtacagcatt gatggggatc ggtgtggcta gtggagaaga
    37981 tcgcgtagta gaagcaacga aaaaagcaat ctcctctcca ttgcttgaaa catctattga
    38041 tggagctgaa caagtacttc ttaacatcac aggcggtttg gatatgacat tgttcgaagc
    38101 acaagatgct tcagatatcg tagcaaatgc agcaactggc gatgtaaata tcattctagg
    38161 tacatctatc aatgaagaaa tgggcgatga aatccgtgtg acggtcatcg caacaggtat
    38221 cgatgaatca aaaaaggaac gcaagtctag ccgaccagct agacaagcac aaatgcaatc
    38281 acctgctcaa aagactgtat tagatatgga tcaagctaaa ccaacttctt cagaagaaga
    38341 aaacagtttt ggcgattggg atatcagacg tgaacaaaat gttcgtccta gagttgatga
    38401 ttccaatttt gacaatatcg aaaaaaaaga atttgataca ttcaaccgcg aagaaacaaa
    38461 atctcatggc gatgatgaat taaatacgcc accgtttttc cgtcgtaaaa aataaaggag
    38521 aggaagagca catgattgct gacaacttgc agaaaatcaa gcaagaaatt caggattcgt
    38581 gtgctcttgt tcatcgtgat ccgaaagaag ttacattagt agctgtaaca aaatctgttg
    38641 aatccgctca agctgaagaa ttggttagag ctggcgttat gaattgtgca gaaaaccgag
    38701 tagataaatt attagaaaag aaacaagatt tatttgattt tcctgaaata aagtggcatt
    38761 taattggtaa tttacagcgc agaaaggtaa aattgattgt aaatgaaatt gattactttc
    38821 atgcgttgga cagtttaaga ttagcagaag aaatccagaa gcggttagag aaagaattat
    38881 cctgttttat tgaagtaaat gtttctggtg aggaatcaaa acatggaatt tcaccagatg
    38941 agcttttacc gtttgtggaa tctttagctg cctgcacaaa aatcaaggtt gttgggttga
    39001 tgactatggc gcctaaagaa gcttctgatg agcaaatcca taaagtgttt ggtacgctga
    39061 aagaattgcg tgatcttgtt caagcaaaag gttatgccca tgcaccatgt agtgaattaa
    39121 gtatggggat gagtcaagac tttcaaattg cgattgaaga aggtgcaaca tttatccgtg
    39181 taggaacagc gctattcaaa gacgaataaa ggaggaacaa aaatgccgct tttaaacaga
    39241 ggagcaattg ctaatttttt cggattagct gatgaggaag agtacgaata ccaagattat
    39301 cctgaaacaa caaaaaaggc tgtacagcca actaaaaaaa atacaacaca gaatcaatca
    39361 actcattctg tatctgctgc gaatactcat gaaaaacaaa aaacggcgac ttcacgtcct
    39421 gtaacgcatg aaagtattca gccggctgaa aagaaaaaag taattgaact acatcaatca
    39481 cctacaaaaa ctgtagcgaa agaaagaaca gcttcaagaa cctctacaca acctaaagca
    39541 aatcttgctg gaaaaatcac tgtaatggaa ccaagagctt attctgaagc tatggcgatt
    39601 gctaaacgaa tcattgctgg agaagcaacc ttagtgaatt ttcatttagt agaagaaaaa
    39661 caagcgagaa gaatcgttga ttttctaaca ggaacagttt atgcactaga tggagatatc
    39721 aaacgtgttg gagatgagat ttttctttgt acaccgactg ggatggagat tgacagttca
    39781 accgctcaat cttttgcaga tgcgaatttg tttgatctgt aagaggagga actacgattt
    39841 atactattgc actattactt catttacttc aacgggcagc ctatttctat agcattatct
    39901 tgatggtata tgcattgctc tcttggtttc caggcgggta tcaatcaggg ctaggacgat
    39961 tattagctaa gatctgcgaa ccgtatatca gtttgttcga tcgtctgcct ttgaggattg
    40021 gacccttgga tttcacaatt ttaggggcaa tcctgatctt gaacttagcc gtacaaggtt
    40081 tagcacagat cgttatatgg ataactgtcc atttagttta ttgattgaag caacaaaatc
    40141 tttattctca gaatagatag gaggggaaaa ttttggatgc aaatgtatac cagcactttc
    40201 gggcagatga acgtacattt atcgattcgg taggcgattg gattgaacag gttcaaggac
    40261 aatatgcacc ctatctgacc gaatttcttg acccaagaca atcctatatt ttagaaacac
    40321 tgattcgaca aagcagtgat ttgcgtttca tgttttatgg cggatatgaa caggcagaaa
    40381 gaaaaagatg tttgattttc cccgattatt acgaacctgt agaggaagat tttgaaacag
    40441 cattgtatga aatccattat cctagcaaat ttgccagcct aagtcatggg aaaatccttg
    40501 gaactttagt gggaacgggg atcaaacgct cttactttgg cgatatcatc tcggacggtg
    40561 atcgctggca agtatttatt gcaaatgagg tggatcattt tgttgctgca caagtaacaa
    40621 agattggaaa ggttgctgta cgcttggaac caatcaaata cacagaaatg attatgccga
    40681 aagatagctg gacacaagaa agaggaacag ttagttccat gcgactagac agtgtgatct
    40741 ctgagattta taatatttcc agacagcggt ccaagcagct gatcgaagct ggtaaagtaa
    40801 aagtgaactg gacagaaaat acaagacctg attttgtact tgaactatta gatattattt
    40861 cagtcagagg ttatggcaga gtccagatcc aagaacttga ggggaagacg aaaaaagaaa
    40921 agtacagggt actgttcggt gtattacgta aataaaataa aaaggtggtt tactcatggc
    40981 tttaactcca ttagatatcc aaaataagac attcccgaca aaaatgcggg gatataatca
    41041 agacgaagta gatgatttct tagatttagt tgttcgtgac tatgaggaat tgactcaaag
    41101 aaatcgagaa ttagaaaaag cagtcaaaca ttctgaagaa aaacttgagt acttcaatga
    41161 attaaaagat gctttgaacc aatcaatcat cgtagctcaa gatacggctg ataaagtaaa
    41221 aacaagtgca agcaaggaat ccgaagtgat cgttacttct gcacaaaaca aagcagatga
    41281 gttagttgcg aacgctgaaa aacgtgcaca tcaattaaca actgatgcag aagaaaaagc
    41341 aagaaaaatc ttgacagatg caacagaaaa agcaagacag cttgcgacag aaactgaaga
    41401 tcttaagaaa aaaacacgag tattccatca acgtatcagc ttgatgcttg agtctcaatt
    41461 agaacaagtg aaaagtccgg aatgggatga aattttacaa ccattctcca gctacgtgac
    41521 tgacagccat gaagtgatca aagaagtttt agcaaaacag cttgacaatg aaaatgatac
    41581 tgatgtaaac tcagacacag gagtcataga agcacctatg acggcatttg atacacaggc
    41641 tattgatatc tcgatcgtgc ctggtgaaaa tgaaaacgag tagaacagaa aagcactttg
    41701 ttattcttaa agcgagccga tgagagtgga agttcggtaa gacactgcat ggtgaaagat
    41761 cctctacttg agacctgctt ttccgaaatt agagtaagaa gcagcggctg acctcgttat
    41821 tggtcacaga gagtttgcac tagatagatg caactaaatt tgggtggtac cacggcatgt
    41881 tcgtcccttg cggctgaaca tgcttttttg tgtccggaaa acaaataaag gagtgtatgg
    41941 ttgaatgaaa atgaaagaaa ccttgcaatt aggaaaaaca aaattcccta tgcgtggtaa
    42001 tttgccaaac cgagaaggcg agtggcaaaa agaatgggaa gaaaatcgaa tctaccaaaa
    42061 aagacaagag ttgaacgaag gaaaacctag ctttgttctt catgatggac ctccatatgc
    42121 caatggtaat atccatttag gacattcatt gaataaaatc agtaaagaca ttatcattcg
    42181 ttcaaaatca atgtctggct atcgtgcacc atatgttcca ggatgggata cacatggatt
    42241 gccgattgaa caagtattgg ctaataaagg cgtgaaacgt aaagaaatgt ccatggcaga
    42301 atacttggaa aaatgtcgtg aatatgcact ttcccaagta gataagcaac gagcagattt
    42361 caaacgccta ggagttgcag gagattggga acatccttat gttacattag atccttctta
    42421 tgaagctgca caagtacgcg tatttggtaa aatggctgaa aaaggctata tttacaaagg
    42481 attgaaacca atttactggt ctccgtcaag tgagtcttct ttagcggaag ctgaaattga
    42541 atataaagat gtaaaatctc catctatttt tgtagctttc aatgtcaaag acggcaaaga
    42601 tattcttgat gaagatacag cttttgtcat ttggacgaca acaccatgga cattgccagc
    42661 aaaccaaggt attgctgtaa atccaaaatt tacgtatgta ttggtggaag ccgatggccg
    42721 taagtttgtc gtagcgaaag atttgctaga aacagtccaa tcagaaatcg gttgggaaaa
    42781 tgtaacagtt ctaaaagaat ttgctggaca agaaatggaa tatatgactg caagtcatcc
    42841 attctatgaa cgtgaatcac ttgtgatttt aggcgatcat gttactttag acgctggtac
    42901 tggattagtc catactgctc caggacatgg ggaagatgac tatatcgctg gtagcaaata
    42961 taaactatct gtagtgtcac ctgtagactc aaaaggattg tttacagaag aagcacctgg
    43021 atttgaaggt atcttttatg acaaagcaaa tcctatgatc actgatctat taaaagaaaa
    43081 aggagctttg ctgaagctag atttctttac tcatagttat cctcacgatt ggcggacgaa
    43141 aaagcctgtg atctatcggg caactccaca atggtttgca tctatcgata aattccgtca
    43201 gaatattttg gatgaagtag aaaaagtaga gtgggttatc ccatggggta aaacacgttt
    43261 gtataatatg atccgtgacc gcggcgattg ggtaatttcc cgtcagcgtg catggggagt
    43321 accacttccg attttctatg ctgaaaatgg agaagcaatc atcacaccag aaacaatcga
    43381 acatgtggca aaattatttg ctgaacatgg atcaattatc tggtttgaaa aagaagcgaa
    43441 agaacttctt cctgaaggat ttacccaccc aggttctcct aatggcgaat ttacaaaaga
    43501 aaaagatatc atggatgtct ggtttgattc tggttcttct catgaagctg ttttacgtca
    43561 gcgaccagaa ttatcattcc cagcagatat gtatttagaa ggatcagacc aatatcgtgg
    43621 ctggttcaac tcaagtatca cgactagtgt ggctatcaat ggtgtagcac catacaaatc
    43681 agttatttct caaggatttg ctttagatgg tgaaggccgt aagatgagta aatctttagg
    43741 aaacatcatt gcaccagaaa aagtgatcaa acaatttggt gcagatatcc ttcgcttatg
    43801 ggtatctagt gtagattatg aagcagatgt tcgtgtttct atggatatct taagtcaagt
    43861 ggcagaagta tatcgtaaaa tcagaaatac gatgcgtttc ttattagcga atacagaaga
    43921 tttcgatcct aaaaaagata ctgtttcgta caatgattta cgttctgtag acaaatatat
    43981 gaccgttcga ttgaaccaag taatcaaaga aatccgagaa gaagggtacg acaaatataa
    44041 cttcatgcat atttaccgta cagttatgaa cttcttgacg gttgatctat cgtcattcta
    44101 cctggatttt gctaaagatg tagtttatat tgaagcagaa gatgattatc aacgtcgttg
    44161 catgcaaact gttttctatc aaacagctgt agctttgaca aaattattga ctcctatcat
    44221 tccgcataca gcagaagaaa tctggtcatt cctgaaagaa gaagaggagt acgttcagtt
    44281 aagcgagttt cctggttatg aagagtttgc aaaccaagaa gaattgatgg atacctggac
    44341 tgctttcatg gacttccgtg ataatgtctt aaaagcattg gaagaagcaa gaaacagcaa
    44401 attgattggt aaatctttgg aagcaaaact aactgtttat ccaaaacaac aagttcgtga
    44461 aatgttaaaa gctttggatg cagatattgc acaattattg atcgtttcac cagactactt
    44521 tgaagttatg gcagcagatg aacaagtgcc agataacgct atggtatttg atgatgttgc
    44581 tattacagtg gaaaaagcag acggggaaac ttgcgatcgc tgtcgccaag ttcgtaaaga
    44641 tgtaggagta gatgaaaaac ttcctcatct atgcggtcgt tgtgcaaaaa tcgttgaaga
    44701 cttctatcct gaagctgtag ctgaaggatt cgaagaaaag taaaagggaa aagggggtgt
    44761 gacatgttct ttgtcacacc cccttttgtt atcaatcgtt taattttcca cgttcacgat
    44821 accagttatc tggttgccag atccattcga atccgtcttt ttcaagtagt tcgaaagctg
    44881 ctttaggtcc cattgatcca gctttgtagt tgggaaaatc cggttgtgtt tggtcccaag
    44941 cttttcgaat gacatccacg atatgccaag attgagcgac ttcttcccaa tgagagaagt
    45001 tcgttccatc accattcaac gcatctaata gtaatttttc gtaagcttct gggctatttt
    45061 caaccatttc tgcactattt ctaaattcca atttgattgg ttccgtttca aatccttgcc
    45121 ctacttcttt cccattcaag gataaagaaa atccttcagt tggctgaata tagatcgtta
    45181 gaacattagg tggaagtgtc gtatcgtcac atggttcatc gacagatgtt ttgaatacat
    45241 taacaggaac ttgtttgaat acgatgttga tccgagtccc tttttcagtc aaacgtttgc
    45301 ctgtacgtac atagaaagga acacctgacc aacggaagtt atcgatagtg aatttgcctg
    45361 cgacgaatgt ttcagtctct gattgagcat ccactttgtc ttcttcgcga tagcccttga
    45421 attgtacacc atccaattca ccttgagcat attgcccgcg aacgaagttt tctaacactt
    45481 cttcttcgtt ataaatgcgg atagctttca atgctttgat cttttctgta cggatttctt
    45541 tttcggaaaa tgctactggc ggttccattg caagtaatgc tacaacctgt aaaatatgat
    45601 tttgcaccat gtctttaagt gcgccactat gatcgtagta gcccccgcga tcttctacac
    45661 cgaggttttc gccaaaagta atttggatat tgtcgatata gcgattgttc cattgagcct
    45721 caaaaatatt attggcaaaa cggatagctg agatgttttg gatcatttct tttcctaaat
    45781 aatgatcaat cctgaaaata tcttgttctg ggaatacttc tctgatttgc tggttcaatt
    45841 caaaagcaga ctggtaatca gaaccgaacg gtttttctat gatcaaacga ttgaagccat
    45901 tttttgtcat aatattttgt gatttcaaat gagaaacgat cgttccaaaa aattgaggtg
    45961 ccatagccaa ataatagaca cggttaccgt ttaattgata gtgtccatct aatcggtcag
    46021 acaattcttt taatgtctga taatgcgttg aatcatttac atcatgggat tgatagtaaa
    46081 aatggcttgc aaaggtagtt gcttcttcat ctgaaggatt caatgcacgg attgtttctt
    46141 tgacaacttc acggtagtgc tcatcgctcc agggacggcg agcggttccg attacagcaa
    46201 aatgctcgcc taaatttcct tttctgtaaa ggcggaacaa agacgggtac aatttacgct
    46261 gggctaggtc acctgtaccg ccgaatatcg taaacaatac atttttttca ttagacatgt
    46321 ggatacactc ctttatattt tatcgacaaa aatcgtgctg gccgccttaa agctgacggg
    46381 gattttcttt ctatcatttt ggatagtgat cggtccttca taagctgcga tgtcaacgat
    46441 ctcatattct tcgttgatat tcaggtcaat agtgactaag taatccaaca attctttttc
    46501 atcaagcact cgtgcgatac gcacttttgt accgacagga taggactcca atgtttgtcg
    46561 gcgttcttca tggacaactt cctctttttt aggaatcgat cctccatgag gacaatgttc
    46621 aggatgattc aaatagtttt ctaaatgatc tgcaagatgt tccgaagtca catgttccaa
    46681 tacttcagca tcatcatgga cttcattcca gctatagtct aaatgttcaa ctaaaaaaac
    46741 ttcccataag cgatgtttgc gaatcagact gctagctttt tgcagtccgc ttgcagtcaa
    46801 ttgtacacct tgataagggg aatgttctac aagctcttct ttcacaagtt tagaaatcat
    46861 ttcactgaca gatgcagctg agacatctag actagatacg atttgtttat tgttgacttt
    46921 cgttgtatct ccacctagtt ctaaaatgat tttcaaataa tcttctcgat ttggtgtcat
    46981 attcgatcat cctttcatgg taaaaaacca tcattagtaa gtgtaacaaa aaaaacgtta
    47041 attgactatt tttctaaata aaaaacaaag gatttttaga cgtttttaca acaaaaaacg
    47101 aaagatgata gagaagactc attctttcgt ttataaagga tatatatata cattgggagg
    47161 gatctatttt tcagataaca acttatatga agtgtctgca gattcgtcat aaagagtttg
    47221 ttcgggataa gaaaagataa gataaaaacc atactgatca cgtacgacta aacgatattc
    47281 atctatttct accacagatg tgccaagaga ctgaccgttg atcaaaaacg tgtaatcaga
    47341 tgtaatctgt aattgttgtt tttgctggct atcactaaag aaccatgtac cttgatactt
    47401 tgagagtcct tctgactttt ccgcttgttt tttctttttc aagtaaataa aacttgagag
    47461 aacggtaatg agtgaaccaa gaaaatagat catttttttt atcaccaaat cactccttta
    47521 tgagaaaata atcgcataga catattcttt cttctttatt tcattgtacc gaataaaagc
    47581 taaaatttct ctaagacttt cgactggttt ttttactgtt tttctacaaa ttatgatagg
    47641 atacatagta ggaagaaagg tgacataatt tgaagtatca gcaagtggaa atagcttttt
    47701 ttattgaccg cccgaatcgg tttatagcac attgtatgaa aaaagacgga gaaataatta
    47761 ttgcgcatgt caaaaataca ggtagaggaa aagaggtcct gataccaggc gctgaagtgg
    47821 ctattgtatt tgctcctgga cctaaacgga aaaccgccta tgatctgatt gctgtaaaaa
    47881 agaagggtga ctggtttaat atcgatagtc aattgcctaa tcggctggca attgatggta
    47941 ttttagacgg cacgatcctt ttaccaggat taactggtcc atttacggta tacaagcgtg
    48001 aagtaactta tcttcactcc aaatttgata tttatttaga gacggtggtc ggacaaaaag
    48061 catttgttga agtcaaaggt atgacacttg aaaataaagc gattggtgct tttcctgatg
    48121 cgcccacact cagaggattg aaacatatag gagaattaac gtatgccgca caagatggtt
    48181 atgccgctta tgtcttgttt attgctcagt tcgaacatct tcatctggca acgatccatg
    48241 aagaaatgca accagcacta gctgatatgg ttcgacatgc gcagcaaagc ggagttcaaa
    48301 tcctcgccta taattgtcaa gtatcggcag atgaggtaac tgtcaagcag gccattccat
    48361 ttgatgtaga tatccctttt gaagatccta tacaatagaa agaaggtaaa ataatgatca
    48421 acttaggtat tatcggaaca aattggatta ctcatcaatt tgtccaagca gctttaagca
    48481 caaaaaaata tgaattgaca gcggtatatt ctagacattt ggaaaaagca caggaatttg
    48541 gttctcatta tgaaggggat atcgaattcg caacagattt gaagactttt ttcaatatta
    48601 ctcatatgga caccatttat attgcttctc cgaatagtct tcactttagt caagctaagc
    48661 aagcaatttt ggcaggaaaa aatgtgatag ttgaaaagcc agctttttcg acgccaaaag
    48721 aaatggatga aatcatccat ttggcaaatg aacacaatgt gtattttttt gaagcagcaa
    48781 gaaatatcca tgaatcaggt tttaaaaaga ttgccgatta cttaccatta aaagacgaga
    48841 taatcggtgc aaatttcagc tatatgaaat attcttcgcg ctatgaccaa gtattagatg
    48901 gagaagaacc gaatatcttc tcgactcatt tctcaggtgg tgcattgatg gatttaggtg
    48961 tttaccttgt atatgcagct gttgcttggt ttggaatgcc aaaagaagtc cattatttcc
    49021 caagaaagat ttctactggt gtagatggtc ttggatgggg gatcttacgt tatgagaatt
    49081 ttgatgtatc gatccagcca ggtaaaatcg gtgattctta tttaccgtca gagatttatt
    49141 ttgactcagg aacgctagtg atgaatggag tcaatgcaat tgaacaagca caatttcatg
    49201 atcgcgcgca taaagaaatc attgatttgg cggtaacagc tgcagaaaat ccaatggcag
    49261 atgaagcaga ggattttgcc aatgtcatgg ctcatccgaa agatccagct tggggattgt
    49321 tgtatgaaga gtggatcgag ctagctagaa atgtgaacca agtgatttat gatttaagaa
    49381 aaaatggcgg catcaaattc gatgcagata atgaggaaga tttttaatga acgaatttgt
    49441 acaagtattt cccgaaaatt ggcagaaaaa atatgaggat aaagggttta tctcaccttc
    49501 tcttattcaa cgagcagttt ttcagccact aagtaataaa caaagtatag tagcggtttc
    49561 tcctacaggt tcaggtaaaa cactggctta tctttggcct ctactactaa acgtggaacc
    49621 aggtgaagca agtgcgttgg tcatttttgc ttcttctcaa gaactagcta ttcaagtagc
    49681 agatgttgca cgtgaatggg gaaaagacaa agagttgaaa gtccaatctt tggtgggcgg
    49741 agccaacgtc aaacgtcaga ttgagggttt gaaggaaaaa ccagaaatct tgattggtac
    49801 accaggaaga atattggaat tgatgaaagc aaaaaaaata aaagcacatc aagtgaaaac
    49861 aatggtattt gatgaagcag accagctatt tgacgctggg aacagccaaa tcattgatca
    49921 aattcttcat caagctccaa cagagtatca gctagctttt ttttcagcga cagcagatcg
    49981 ttcccttgaa agcattgaaa aaatcactgg gaaaacgttt gagacaattg acgtgacagc
    50041 agaggacgat tctcgaaaag gactgagaca ttacttttta cgtgtaccga tacgaaagaa
    50101 agatgactac ttgagacgtc tcacgcatat agaagatttt catggtctga ttttctttaa
    50161 tcaactgact gaattaggcg taatggaaga aaaattgctg tatagaggtg taccggtagg
    50221 aagtctggcc tctgatcaga acaagctact ccgaaaagca gcgctagaac aattcaaaaa
    50281 gaaaatggtc agagcacttt tgaccacaga tgtggcggct agaggtttag atattacagg
    50341 tcttccttat gtagtgaatg cagaagttcc gttaagtgaa gaagcttatt tgcatcgttc
    50401 gggaagaact ggtcgaatgg gtaatgaagg aatagtaatt acactcgtac aagataataa
    50461 tctaagagat ttaaaacggc ttttacgtcc atcaaatata acacttgaag agattttttt
    50521 atacggaggt aagctgcaaa ctgagcagcc aaccaaagaa gatgcagctc ctgcagtaaa
    50581 taaaaagtat gcgaaaactt tttctggaaa ggagcagaac ccagaatata acaaagaaaa
    50641 aataagaaaa aaagttaaaa ataaatcaaa aaaagagaga aataaaggag caaggcgcaa
    50701 ataagcgcct ttttatgcta tgattatgaa aacgggaaca aaattggttg ccagaggagg
    50761 ggttatgtga aagaacgtca agaagagatc ttgcagctat tgacagaaaa ccaacaagga
    50821 ttgacagcaa gtgatgtagc tgagcgctta actatcgatc gaagcaatgc aagccgatat
    50881 ttaagtgaac tatacaaggc ccatcatatt gtaaaaaccg ctggccgacc agtcgtttac
    50941 tctttgccaa cggaaaagtc aaaatctgat gaagtgcatg tcgactcttc tacgcaagtg
    51001 acttttgaaa cactcgttgg agaaaatgat tctttaaaag tcagcataca gcaggcaaaa
    51061 gctgcgattc tttatccacc gcgggggttg catacgatta tttttggaga aactggtaca
    51121 gggaagtcga tgtttgctga atgtatgtat cattttgcaa tcgattcaga gatgctttct
    51181 gcagatgcgc catttgtttc tttcaattgt gcggattatg ctcaaaatcc gcaactgctc
    51241 tttggacaca tctttgggat caaaaaaggg gcttacacag gagcagctca agatagtccc
    51301 ggtctaatag ccaaagcaga cggcggtata ttgtttttag atgagatcca ccgtttaccg
    51361 ccggaagggc aggaaatgct gtttactttc attgataaag gtatttaccg tccgcttgga
    51421 gaaagtgcac aagtacatga agcatctgtt cagatcattg gggcgacgac tgaatcttca
    51481 gaaagtttct tatctacgtt caatcgacgg attcctatgg caatcacctt gccgaatctt
    51541 gcttcacgtt cactggatga gcgttatgaa atcatctctc tttttatcaa acaagaagcg
    51601 aatcgtttga atcaacgtat cgatatcgaa aaagaagcga ttcttgcctt catgctttat
    51661 gatgcggaag gaaatatcgg tcaagtgaaa cgagacttga aactagtctg tgcaaaatct
    51721 tttttacatt accgtactca tcatgagaaa aagctaatca ttcgaaaaga agaactttcc
    51781 cttcaagtac aaaaaggatt actgaaaatc aaggaaatac cggaacgtct cgaccgattc
    51841 atggacagca aaagtcagta tttgacattt gaaccaggat ttgctgatgt tgtctggtca
    51901 caagatccag aacgaaacat gcaggtctat aacgatattg aggaaaaagt cacttctctt
    51961 tccgaaaccg gtgtggagaa cattgatcta gagaccctca tttcaaaaga tgtggatgct
    52021 tatttccaga catatgtcaa agaattgact aaaagttcga ttcccaaaga tcttttgcca
    52081 gatgaaatct ggcggttaac caatcaattg tatgatgtgg ctgaaaaacg gttagaccgt
    52141 atttataatg aaaaagctcg ttttgctttt gctttgcatc tgcaaagtac attggatcgt
    52201 gtgagagaag gtcatatgat cgtccaccca gatttgaata acgtacggaa aaattttaag
    52261 agggaattcc aggtagcaat cgatctttca acgatcattg aagaagaaga acaagttgag
    52321 attccttttg atgaaatcgg ctttattagt atgtttcttt ctatcaatct aggagaaagt
    52381 gagcctgtta aagaaaacaa agtagatgtt ctcatattga tgcacggcag acagactgct
    52441 tcaagcatgt tggagatggc acaagaacta ttaggaacaa aagtagggaa agcttttaat
    52501 atgccactgg ttatggaagt tcaaacaatg tatgaagaag tgttacaata tgtcaaaaac
    52561 tatcaagagc atttgacgaa tggcctactc ttgttaacag atatgggatc attaaacacc
    52621 ttcagtgatt tgatttatga agagactggc attcggacga aagctatcac gatgacaagt
    52681 accatgattg tgatagaagc gatacggatg gcaagtgttg gaagaagctt agaagatatt
    52741 tatcaaaata tccagctttc ctttgaatcg atcgtacgtg atcagttcaa agcggaaaag
    52801 tctgaaactt tacagcgtaa aaaagccgta atcgtgacat gctttactgg tgaaggagta
    52861 gctgccaaat tatatcagcg tattatgcca gtgattaatc aaaataaggt agaactgatc
    52921 cagatgcagt ttttagaacg tgaatcattc aaaaaacaca tcgatgcctt attggaagaa
    52981 tatgagatca aagcaattgc gggaacagtt gatgttgatt atcaaaatat cccatttttt
    53041 tcagcctatg acgtttttga tgatgaaaaa ctaaatattt taaagcgtat agctgatgat
    53101 gagatcccga cagctaaaat tgtcgattca cttgctggat cattaagcga tgtcttttct
    53161 attgatgaac tgatccgttc attgcaaaag acagttcagc agatccagac cgatttgtcg
    53221 attatcgtgg aacctggagt agatactgga atcgttatcc atttagcctt catgttagac
    53281 ggattgatca aacaagaaag ccatcggcaa tttgagaaac tgtctatttt ctcaaaaatt
    53341 tatcgtttgg aaatggatat cgttcgggca aacttgatgg cactagaacg aaaatatcag
    53401 gtaaaaattc cagaagatga tatcgcattt cttacacaaa tgtttcttga aaataaaata
    53461 aaaaaatgaa cacactaatc atactgttta cacactaaac atctgtgtgc gcactataaa
    53521 aaagagctaa aaagcatgaa aacgctattt cagctctttt ttttattggc atgcttcttg
    53581 cgttatataa aagtgtaaaa aaataaaagg agtgttaaaa atggcaaaaa aaacaatcat
    53641 gttagtttgt tcagcaggga tgagtacgag tttattggtc acaaaaatgc aaaaagcagc
    53701 agaagaaaaa gggatggaag ccgatatttt tgcggtctct gcctcagatg cagataataa
    53761 tttagaatct aagaatgtcg atgtattatt attaggtcct caagtacgtt tcatgaaagc
    53821 tcaatttgaa caaaaacttg cgcctaaagg tattccatta gatgtcatca atatgcaaga
    53881 ctatggcatg atgaacggag aaaaagttct tgcacaagca gaaaatctaa tgaaataaat
    53941 caagtatgca gttgggggta taacaacgtg gaagatcaac aaaatctaga agccatcatg
    54001 gggttaatca tgtttggcgg taatgcaaag agcgatgcaa tggaagcgat tgcggctgct
    54061 aaaatcggtg attttgagtt agcggatagg aaaatcgctg atgcagaaga atcacttata
    54121 caagcgcatc atgcacaaac agacatgttg acacaagaag cacaaggaaa tcatatgcaa
    54181 gtgacattat tgactgttca cagtcaagat cacttaatga catctattgc gtttactgat
    54241 ttagcaaaag aaatcatcga actttatcgt cgcgtataca gcgaataaat acaacttctt
    54301 ttttgaacgg aagaagaaaa atttttgaac ggatttttta ttttgttata aggagaactc
    54361 tctttatata aaaaacttat aggaggaagt aaaaaatgaa cggactatca agttggatgg
    54421 agaaatacat cctgccggtt gccgcaaaaa tcggagcgca aaaacattta gttgctttgc
    54481 gtgatgcttt tatcggcaca ctgcccgcaa caatggcggg atcagtcgct gtaatgatca
    54541 atgcaattat tcgtgattta ccgcaacagt ttaacagtac ctatgctgac agtttagcag
    54601 cagacaaagg aatctttgcc gtattgaatg tcatcatcgg aatcaatggt tttgtctgga
    54661 acggtacact agcaatcgcc ggattgatct ttgccttttc ttgggggtat aatcttgccc
    54721 gagcttatgg agtaaatgat ctagctgggg ggatcgttgg tttagctacc ttgattcaag
    54781 gtatttcctt tggtccagca ttatcagcag cattagatgt gaatgttcct tcaaatgtcg
    54841 cagatgcaat caatagttct gagattggtg caacgattgc agatggcaca ctgtcagttg
    54901 gattatgggg ttggctaaaa ctagatcatt tgaatggaaa cgcttatttt acagttatga
    54961 tcatgggagc tatttcagtc attatttttt gtaaattgat gcttgccaac attacgatca
    55021 aaatgcctga ttcagtacca ccagcagtat ctaaagcatt tgcagccatt ttaccagcga
    55081 cgatcgcttt atacgtcatt gctattatca attatatcgt aggtcgtgta gctgatgggc
    55141 aactgatcat tgatttggtg caaaaatata ttgcagaacc attcctagga ttatctcaag
    55201 ggttgggagc agtattgatc gtcacagtat ttgttcaagt attctggttc tttggtattc
    55261 atggaccaaa tgtattggca ccagtgctag agggaatctg gggacaagca caactaatca
    55321 atattgatat tttccaaaaa ggttataacg gaaaaactgg tacaccagcc gtgttagatg
    55381 caatcgaaga tggaaaagct tatatgtggg tccgcggatc gttcgatgct tttgcatggt
    55441 ttggcggatc aggcggaacg atcgtcttac tgatagctat cttgattttc tctaaacgtg
    55501 cagattatct aacagtagct aaattatcca ttgggcctgg tatcttcaat atcaacgaac
    55561 cagtgatgtt tggtctgcca atcgtattga atgcaatcat gtttatccca ttcttgattg
    55621 ctccagtagt agcaacagca attggatgga cagctactta tcttggccta gtcgctccgg
    55681 tatcgcagca agtcacatgg gtagtaccgc cgttcttatt gtcattctta gcaacaggag
    55741 cggattggcg ggcaccgatc gtaacattag tatgtatggt cgtgactttc ttgatttggg
    55801 caccatttgt catctctgca aacaaaatgg accctgccgc aggcgaacaa caataagatg
    55861 aacgtgagga gcgggacgct ttaagtttcg ttcctcataa tgaattttct aggaggaact
    55921 gaaatgatta caattgccag tgcaatgcaa caagatgaaa tcaaagaatt gttagaagga
    55981 tacaatgaag aaggaatgac ttttacattt aaagaaaaac aaggaatcaa gctgttcttt
    56041 gaaacaagtg cagaagatcc ggaagttgcg gctaaaaaag caaaggaact aatcaaagca
    56101 cagccttggg gaacagtcct ttattttcaa tcaacagctg tttagaaaat ggtttaaaaa
    56161 gataggatgg ttgagatgat aaaaaacggt ttatttttat gtagtattgg ttctttgatc
    56221 gtgttgtata gtttgaatcc acattcgcaa aggtatgatt tgtatagtat ggtcacaggt
    56281 ctttttctaa caggattagg aatctatttc ttcatgaagg ggaaaaaaag agaagaagca
    56341 aaggagaaaa ataactaatg gaaatcacga aaaaattaaa tattccagca tcgttttttt
    56401 acgataaagt tatggactct gtccttttcg atgtacgtaa agcgaccggc aaatcagtaa
    56461 ctagaaaaca gctgaaaaat ttggagtacg tcaaacaatt ctcgcaaaat aaccgggctc
    56521 gcataaaaat cgaagaagtc attgaaaacc agtcttataa gtttcgtaca tcgacaacga
    56581 agaatgaatt cgtcgttcat tatcaaatca aaccattgga cgattcgacc tgtgaggttc
    56641 attacacaga aaaaatggag tcttatggca tgcttcaaaa gttgaatgat atgcttttag
    56701 gaacaatctt gatgtatttt aaacggagac gtttcaaaaa aatgctagat atgatcgaag
    56761 caacgtatta aatcaatgaa acatggtact atttagttat tgaaaaatga agtaagctag
    56821 aaggaatttt tcataggaag atagatcttt cctgcgaatc ctcactagct tttttgtagg
    56881 aaggggcagc aaaatgacaa acaaagtaag agagctaggt gtatcttcga ttgaggaaat
    56941 gtttgattta gccacttatg catttaatgg ggcagataca cctgaaaggc gagaacgttt
    57001 tcgtacactt gcggagaatt cttggaatta cggatttttt gatgaggagg atcggctaac
    57061 aagtcaagtc atggcgaccc cttttccagt gaatttttac ggacaagagt acctaatggc
    57121 cggcattggc tatgttgctt cttatccaga agcaagggga caaggcggga tcaatcagat
    57181 catggaaaag atccttgaag attgccgtga tcgtaaagta agcttatctt atctggcgcc
    57241 attctcctat cccttctatc ggcgctatgg ctatgaacaa tcatttgata aaatcagtta
    57301 tcaactaaac agtcgtgatg tgccgtttat caaaaaaaaa tctggaaaaa taaagcgtat
    57361 ggattttgaa gatgcaaaag ctgcaatcag aagaatttat gaacagatgc cggaaaacca
    57421 acgagctggt ctgaagcgcg aagactggtg gtatacgtat aaattcaagc aaaaaggcaa
    57481 ttaccagttt gctttgcatt ttgatgataa tgaggaagca acgggatata tcgtttatca
    57541 gcttgcaacg tcttccttta ttattgcgga gtggggattt ttggatcatg atgctttttg
    57601 cagccttgtt cgcttcgtaa gttctcacaa tggtgctttt gaaacatttg aatattcatg
    57661 cggctacggc ggtaatgatc aaaattatct attagaaaat ccatttgctt ctgtaatgat
    57721 cacaccttat atgatggcga ggatcgtaga tatttctttg tttttggaaa attatccatt
    57781 taaaaagaga caagctgcat ttgccctaca aatagaagaa gatcattatg cgaaatggaa
    57841 tgaaggaatc gtggaagtaa caatcgaaaa tggaaaaaat catctctcaa aagtagaaca
    57901 aacaaaactc ccaatcgttc gaggaagtat ccaacatatc acgcagctat tattagggta
    57961 tcgtaaagtg gaagagttgg tattccaaga aagaatacag gtgacaaaag aatatgaaga
    58021 aacgttaagc tcaatcttgc cacagcaaca gccaattctt aatgactatt tttgataaaa
    58081 aacgggtgat gacaatattt ttgtcaccac ccttttgttt ttcatttggc atttgaagga
    58141 aaatgttaaa gtaaatcctc ttttttcgta aatttgaatt ttttgttttc ttttgtttta
    58201 gggttttctt ttttgtggtc ttggattgct tctttcgtaa tcttttcccg atttttttta
    58261 gatgaatcta agcgttctgc gtatactttt tcttttggct tatcattcat tggaaaggac
    58321 ctccctacat tatttcattc tacctctagt ttatcatctg ttaaggacta gtggaaacgt
    58381 tttgcctaac ttataagctg atttttttag cagataagct tgcaagtaaa aaacaaacaa
    58441 gctataattg atttattgaa attgttcctg tagctcagta ggatagagca atcgcctcct
    58501 aagcgatagg tcgctggttc aattccagtc aggaacgtat aaataacccc tgtatccttt
    58561 gcggtacagg ggtttgtttt gtatttgtgc caaaaatgtg ccaaactagt tatcattttt
    58621 atataaacta tcgaacacag tattgatttt ttctttatcc tgatcttcaa gctctcgaac
    58681 aatatgaaga taagtagaca ttgtagtttc taaactgcta tgtcctaaac gtttcgatac
    58741 gctaagtata tttactcctt gataaagtaa aatagatgca tgtgtatgcc ttagtccatg
    58801 caaagtaaat tgcttttcaa tccctaattc ttttaatttt ttgcgcaaat atttgctgac
    58861 ggcgtttgat gaaactaatc cattttttaa gttgaaaaaa acaaaattat gagggttttt
    58921 gatttcaaaa ttctcgaata attctttttg attgatttta aactttttta agagatcaat
    58981 cgtatgttta tcaatggaaa ttttcctatt tgaagtttcg ttttttgttt ttccccattc
    59041 atttaattta taattccatg ttttattaat agtgatgatt tgatcttcga aatcgatatc
    59101 ttcccatgtt aatcctagaa gttcagcaaa tcgcattcca gtggcaccag caactaacac
    59161 gagcatagga gaagagtact gcgcagaaag gttttcttct acgagtttca ttaaagattt
    59221 aaattgatca taatctaaat atttatcttc ttttggcttc aatgaatctt ttcctttgat
    59281 tactgctttt ctagttggat caaacggaat taatccttct tctacggcat ctttcaacga
    59341 cgctctaata tggttattaa acttaataac ggtagatttt acatggtctt tagcatactt
    59401 atttaaaaag cgttgatatc ccactctatc taaatcagaa atcaataccg ctggcatata
    59461 tttctttatg ttcattaacg tatcttcgta ttttctataa gtgataggag atactgttcc
    59521 ttctttataa agttgcatcc aatcctcaaa gtaatctgat aataatagat ttttgcgatc
    59581 ccctttaagg cctttgttca agttatattc taattcgttg gcggcatctt ttgcttctcc
    59641 ttttgtctta aatcctgatt ttcttatctt cgtgtatttt ccgtcatctt ttttgtatga
    59701 aatttcgtat tgccaactat ttccacgttt gactaatctt gccataattg aatcagctct
    59761 ctttctctga tacaatagga actataaaga agcccattgt ttgggtttta ttattttttg
    59821 cacatccttt ttgcttgccg gcgcaaggat gtgctatttg tcgttttaat ggtataattg
    59881 gatgtgtatt agatcatcca atcagaggtg tttattatga ttaaagtgat ttttaaaaga
    59941 ataactgaac ttccagagtt tagattattt gttgattatt ggtatgtctg gttttttatt
    60001 gttgcattgc tgataggtct agatgtcata ttagaaagaa aacttaagta agagttgagt
    60061 taataaattg aattcctaaa tgagatattt tgtagcccat attttgtttg ccgacatcac
    60121 ctaatactat ggagctaatc gttttttcta acagaccaaa atcagataat ctatttaagc
    60181 taccatctat tagctcttgt gaatagccag acatgctgat atttccagtg aaattttcgt
    60241 ttccacgact cattaagcat gctaacaatt gaatctcaat cgatgttaat tgattcagta
    60301 caagaagata atactcctcg ttatcccatg aagcgctgtt aattcgaagt aaacaattct
    60361 tataaagatt tttatacata tctattttat tttgtgcctt tgatttttct acctcgtcat
    60421 ttatcccttc gaatattcca atgagttggt ccttgttctc taagttgaaa acatcttctg
    60481 gaatttgatc gcgaatgttt tctaatgatt cgtttaaatt tttgtagaat ttctcgatgc
    60541 gtttgaaacg cttctcgttt ttagacccaa aataaagtga ctgcacaacg ccaccaatat
    60601 aaggaattgc tccaataccg ccttcaacta ttaattcaag atattctttt ttggttagtt
    60661 tttcattgct attatccata gttacctcct attttgtcat ttcctttaca agcattgtga
    60721 ctcctgatgg cactcttttg tataggtttt attattgtgc acaatctaac tcttagtggg
    60781 gcgtggttca tttatttttt acttacttta ctattaatca tatcccattg agcataggat
    60841 ttttctatgt tcgatcctct tgaactaatt tctcctgtaa aagttacttt atcgcccttt
    60901 gtaaaggtat cagcattgtt taagtccttc gcaaaaatta catatgaaac tttaggtgtt
    60961 gcagaaatat cattccaact tttatcatcg tactctttgt ctgctataat ggctattcta
    61021 gtagtcattg attcgataac agttccttca aaagtatatg ttttaccact aatatattta
    61081 gtaaaagctg tattttgctc agtagctgga gtaatagaat aataactatc taagaaagca
    61141 ccccagtcat gattatttgt ctctaaaaat tcttcaaatt gagttaggct tttattgctg
    61201 ctgttagatg tagtagtttg agtatcagat gatgagcttt cctgtttgtt attgtcattt
    61261 gcctcgtttg atgaggaaag cgagaaacct acggtagtaa aaataatccc gataataagg
    61321 agaatcatag gtgttttctt atttgttttt ttgata
//
LOCUS       DACDEG010000008        60449 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.008, whole genome shotgun sequence.
ACCESSION   DACDEG010000008 DACDEG010000000
VERSION     DACDEG010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.008"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            292..1431
                     /locus_tag="IV142_000553"
     CDS             292..1431
                     /locus_tag="IV142_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP8484237.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1498..2073)
                     /locus_tag="IV142_000554"
     CDS             complement(1498..2073)
                     /locus_tag="IV142_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8484238.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2078..3052)
                     /locus_tag="IV142_000555"
     CDS             complement(2078..3052)
                     /locus_tag="IV142_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAP8484239.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3076..3120)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3335..4303
                     /locus_tag="IV142_000556"
     CDS             3335..4303
                     /locus_tag="IV142_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8484240.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4416..4694
                     /locus_tag="IV142_000557"
     CDS             4416..4694
                     /locus_tag="IV142_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP8484241.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4812..5048
                     /gene="secG"
                     /locus_tag="IV142_000558"
     CDS             4812..5048
                     /gene="secG"
                     /locus_tag="IV142_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP8484242.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5160..5906
                     /locus_tag="IV142_000559"
     CDS             5160..5906
                     /locus_tag="IV142_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8484243.1"
                     /translation="MKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSLAEH
                     QLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5909..8266
                     /gene="rnr"
                     /locus_tag="IV142_000560"
     CDS             5909..8266
                     /gene="rnr"
                     /locus_tag="IV142_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP8484244.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8288..8752
                     /gene="smpB"
                     /locus_tag="IV142_000561"
     CDS             8288..8752
                     /gene="smpB"
                     /locus_tag="IV142_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP8484245.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9054..9773
                     /gene="atpB"
                     /locus_tag="IV142_000562"
     CDS             9054..9773
                     /gene="atpB"
                     /locus_tag="IV142_000562"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP8484246.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9826..10041
                     /gene="atpE"
                     /locus_tag="IV142_000563"
     CDS             9826..10041
                     /gene="atpE"
                     /locus_tag="IV142_000563"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP8484247.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10132..10656
                     /gene="atpF"
                     /locus_tag="IV142_000564"
     CDS             10132..10656
                     /gene="atpF"
                     /locus_tag="IV142_000564"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP8484248.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10643..11185
                     /locus_tag="IV142_000565"
     CDS             10643..11185
                     /locus_tag="IV142_000565"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP8484249.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11208..12764
                     /locus_tag="IV142_000566"
     CDS             11208..12764
                     /locus_tag="IV142_000566"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP8484250.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            12778..13680
                     /locus_tag="IV142_000567"
     CDS             12778..13680
                     /locus_tag="IV142_000567"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP8484251.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13703..15109
                     /gene="atpD"
                     /locus_tag="IV142_000568"
     CDS             13703..15109
                     /gene="atpD"
                     /locus_tag="IV142_000568"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP8484252.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15123..15545
                     /locus_tag="IV142_000569"
     CDS             15123..15545
                     /locus_tag="IV142_000569"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP8484253.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16014..16244
                     /locus_tag="IV142_000570"
     CDS             16014..16244
                     /locus_tag="IV142_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP8484254.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16341..17642
                     /gene="murA"
                     /locus_tag="IV142_000571"
     CDS             16341..17642
                     /gene="murA"
                     /locus_tag="IV142_000571"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP8484255.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17655..17831
                     /locus_tag="IV142_000572"
     CDS             17655..17831
                     /locus_tag="IV142_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP8484256.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17966..18262
                     /gene="yidD"
                     /locus_tag="IV142_000573"
     CDS             17966..18262
                     /gene="yidD"
                     /locus_tag="IV142_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP8484257.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18326..18793
                     /locus_tag="IV142_000574"
     CDS             18326..18793
                     /locus_tag="IV142_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8484258.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            18878..19762
                     /locus_tag="IV142_000575"
     CDS             18878..19762
                     /locus_tag="IV142_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8484259.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19879..20112
                     /locus_tag="IV142_000576"
     CDS             19879..20112
                     /locus_tag="IV142_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP8484260.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20200..20280
                     /locus_tag="IV142_000577"
     tRNA            20200..20280
                     /locus_tag="IV142_000577"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20234..20236,aa:Tyr,seq:gta)
     gene            20287..20358
                     /locus_tag="IV142_000578"
     tRNA            20287..20358
                     /locus_tag="IV142_000578"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20319..20321,aa:Gln,seq:ttg)
     gene            20873..21580
                     /locus_tag="IV142_000579"
     CDS             20873..21580
                     /locus_tag="IV142_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8484261.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(21639..22273)
                     /locus_tag="IV142_000580"
                     /pseudo
     CDS             complement(21639..22273)
                     /locus_tag="IV142_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
     gene            22400..22687
                     /locus_tag="IV142_000581"
     CDS             22400..22687
                     /locus_tag="IV142_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484262.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            22795..23115
                     /locus_tag="IV142_000582"
     CDS             22795..23115
                     /locus_tag="IV142_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8484263.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            23196..24509
                     /locus_tag="IV142_000583"
     CDS             23196..24509
                     /locus_tag="IV142_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8484264.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKVCQEIKEMNEEGKKML"
     gene            24506..25225
                     /locus_tag="IV142_000584"
     CDS             24506..25225
                     /locus_tag="IV142_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP8484265.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYRWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            25353..25904
                     /gene="raiA"
                     /locus_tag="IV142_000585"
     CDS             25353..25904
                     /gene="raiA"
                     /locus_tag="IV142_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP8484266.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            26126..28660
                     /gene="secA"
                     /locus_tag="IV142_000586"
     CDS             26126..28660
                     /gene="secA"
                     /locus_tag="IV142_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP8484267.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            28786..29887
                     /gene="prfB"
                     /locus_tag="IV142_000587"
     CDS             join(28786..28848,28850..29887)
                     /gene="prfB"
                     /locus_tag="IV142_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP8484268.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            29992..30678
                     /gene="ftsE"
                     /locus_tag="IV142_000588"
     CDS             29992..30678
                     /gene="ftsE"
                     /locus_tag="IV142_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP8484269.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            30662..31555
                     /locus_tag="IV142_000589"
     CDS             30662..31555
                     /locus_tag="IV142_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8484270.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            31707..33155
                     /gene="cls"
                     /locus_tag="IV142_000590"
     CDS             31707..33155
                     /gene="cls"
                     /locus_tag="IV142_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP8484271.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            33492..34349
                     /locus_tag="IV142_000591"
     CDS             33492..34349
                     /locus_tag="IV142_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP8484272.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            34464..35384
                     /gene="pstC"
                     /locus_tag="IV142_000592"
     CDS             34464..35384
                     /gene="pstC"
                     /locus_tag="IV142_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP8484273.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            35384..36268
                     /gene="pstA"
                     /locus_tag="IV142_000593"
     CDS             35384..36268
                     /gene="pstA"
                     /locus_tag="IV142_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP8484274.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            36283..37089
                     /gene="pstB"
                     /locus_tag="IV142_000594"
     CDS             36283..37089
                     /gene="pstB"
                     /locus_tag="IV142_000594"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP8484275.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            37105..37863
                     /locus_tag="IV142_000595"
     CDS             37105..37863
                     /locus_tag="IV142_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP8484276.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            37876..38553
                     /gene="phoU"
                     /locus_tag="IV142_000596"
     CDS             37876..38553
                     /gene="phoU"
                     /locus_tag="IV142_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP8484277.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            38804..39822
                     /locus_tag="IV142_000597"
                     /pseudo
     CDS             38804..39822
                     /locus_tag="IV142_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723340.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
     gene            39819..40586
                     /locus_tag="IV142_000598"
     CDS             39819..40586
                     /locus_tag="IV142_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP8484278.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            40686..42254
                     /gene="liaX"
                     /locus_tag="IV142_000599"
     CDS             40686..42254
                     /gene="liaX"
                     /locus_tag="IV142_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP8484279.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            42554..42871
                     /locus_tag="IV142_000600"
     CDS             42554..42871
                     /locus_tag="IV142_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP8484280.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            42871..43221
                     /locus_tag="IV142_000601"
     CDS             42871..43221
                     /locus_tag="IV142_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP8484281.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            43388..44326
                     /locus_tag="IV142_000602"
     CDS             43388..44326
                     /locus_tag="IV142_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP8484282.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            44341..45171
                     /locus_tag="IV142_000603"
     CDS             44341..45171
                     /locus_tag="IV142_000603"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP8484283.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            45215..46240
                     /locus_tag="IV142_000604"
     CDS             45215..46240
                     /locus_tag="IV142_000604"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP8484284.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            46255..47193
                     /gene="galU"
                     /locus_tag="IV142_000605"
     CDS             46255..47193
                     /gene="galU"
                     /locus_tag="IV142_000605"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP8484285.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            47318..47758
                     /locus_tag="IV142_000606"
     CDS             47318..47758
                     /locus_tag="IV142_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP8484286.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKATAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            47761..48363
                     /locus_tag="IV142_000607"
     CDS             47761..48363
                     /locus_tag="IV142_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP8484287.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(48422..49528)
                     /locus_tag="IV142_000608"
     CDS             complement(48422..49528)
                     /locus_tag="IV142_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP8484288.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            49738..50739
                     /gene="ccpA"
                     /locus_tag="IV142_000609"
     CDS             49738..50739
                     /gene="ccpA"
                     /locus_tag="IV142_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP8484289.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(51076..53421)
                     /locus_tag="IV142_000610"
     CDS             complement(51076..53421)
                     /locus_tag="IV142_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP8484290.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSISDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLSKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            53700..54602
                     /locus_tag="IV142_000611"
     CDS             53700..54602
                     /locus_tag="IV142_000611"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP8484291.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTAAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            54815..56590
                     /locus_tag="IV142_000612"
     CDS             54815..56590
                     /locus_tag="IV142_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP8484292.1"
                     /translation="MLNTLKPLINQLTKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHFSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(56656..58242)
                     /locus_tag="IV142_000613"
     CDS             complement(56656..58242)
                     /locus_tag="IV142_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP8484293.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            58493..58717
                     /locus_tag="IV142_000614"
     CDS             58493..58717
                     /locus_tag="IV142_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP8484294.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            58859..>60449
                     /locus_tag="IV142_000615"
     CDS             58859..>60449
                     /locus_tag="IV142_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8484295.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIAMIPLPLLAITSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYLKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVKHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLMLDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAH"
BASE COUNT        19755 a        10637 c        12908 g        17149 t
ORIGIN      
        1 tttttttgca actagcttct gtatatgatg tttacaaaga atggtacaag aaaagatcat
       61 ccttgaaaaa ggaaagctaa caataactag catcatataa gagcttttaa ggatagaaca
      121 gtagaatagg aatctatggg caagtcctct cgtataaata aaataggagg gaagtagcag
      181 catatttttc ataaagtggc tctctttctt catttttact ttggttgctc ctatttgaaa
      241 attcgagcac actcaaatac aataaaaaga aatggcaagg gagaggatta gatgaaaaaa
      301 gaagaaaaaa tcgcgatttt acaagagatt atccgaatta aatcagtgaa tggaaacgaa
      361 ggtgaagtag cagcctattt gaataagctt ttggcaaggc atgacataac aggagagatt
      421 gtttcttaca gagatggtag agataatttg attgctcgtt accaaaaggg ccaatcagga
      481 aaagtgttag gcctatctgg acatatggat gtcgtggcgg ctggtgatga atcgtcttgg
      541 acctatgcac cctttgcagc agaaatccat gggaaccgtt tgtacggacg tggtgctaca
      601 gatatgaaga gtggcttggc agcaatggtg attgccatga tcgaactgaa agagtcagga
      661 aaaccattta acggaacagt caaattatta gccactgtcg gagaagaagt gggtgaacta
      721 ggtggggaac agctcacgaa agcaggctat gtagatgact tagatgcctt gatcatcgga
      781 gagccaacca attacagttt gatgtatacg cacatgggat caatcaacta tacagtcacc
      841 tcgcacggaa aagaagcgca tagctctatg ccagaccaag gttataatgc aatcaatcac
      901 ctaaatgaat tcattacgaa agcaaatgct gaaatgaatc acctcgctga aacaatcgag
      961 aatcctgtcc taggaaaaac aatccataat gttacattga tcagtggcgg gaatcaagtc
     1021 aacagcatcc caagccatgc gcagctccaa gggaacattc gatcgattcc agaataccca
     1081 aatgataaaa tcattgcatt gctgcaatca atcgtcaatg aattgaatca agagacagac
     1141 tatcatttgg aattgatgat cgattacaac aaaatacctg tcaaagctga tccagattct
     1201 cctttgattc attccattca gcaacaattc agccagccat tacctttagt tggagcggca
     1261 gcaacaacgg atgcagccga atttacgaaa gctaaccatt ccttcgactt tgtcgtcttt
     1321 gggcctggtg tcgttacttt gcctcatcaa gtagatgagt atgttgaaat cgataattat
     1381 ttggatatga tcgaaaaata tcaaggaatt attttatcct acttagcgta agattttttt
     1441 aataaatgaa aaaacagagc cagattagca ttattttgct aatccagctc tgtttattca
     1501 gcctattttt ctttttgaat atatcaagcg ttggggcaac atagtgatga ctaatgaaac
     1561 aacgacgata gacaacaagc gatcaaggta gtctgtaccc acctgtacca agatcacact
     1621 tgttgtctga cttaaaccta acccatgtaa tagctgaacg atcatactgg atcctgagga
     1681 ggtgatcccg ccaaataact gaacggtaat gatcgaagaa acgatcgttc caggtaccga
     1741 caaaatcaaa gcgagtaata acagtttcaa gctgttttta tctttgccga gatatcttgg
     1801 caaaatcaat ccagcaagca agcctgtgag aatttggatt ggcgaataat acagagaaaa
     1861 aatatctgta gtcactccac ttaaaatacc gctggacaat cctgtcagca ttccaggtac
     1921 aggcccaagc aatgcaccag caaaaatcgt tccgatagaa tcgagataga ttggcaaacg
     1981 aagccataaa gcaatcgttc ctccaatata attgatccca atacacaaag ccatcactgt
     2041 aagtacgcga gttgccattt tgtttttatt cattttgtta gcccatcttt aacctttcca
     2101 aatctttcat aatcgtttct tcttgtgcat ttaaaagaac ggttaaaaac tttcggaaaa
     2161 aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc caaaattcat
     2221 aacgatcaac caatgtttgt ccgcgactta ttccttgagt ttctacagca gtgaagcttt
     2281 taaagccttc acagatggac tcatcgataa aataagcaac agctaatgga tcattgatga
     2341 cacatcctaa gattctttct tgtttccagt gaaagtcaaa ataaaaacga gtgatcgctt
     2401 ttagatactc tccctcttca ggattcattt ggcagcaata ttcaaggata gtcggtgtca
     2461 agacgatttc tcttgtcaca tctaacccaa ccatttctat cggttgtttg agcttttcga
     2521 atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact ggtgaacagt
     2581 taccatggct tttgtaagtt ccgcccattg agacaaaacg atccatatgt ttcccaacag
     2641 atgggctgtt tttcaaagca gaagcgatat tggtcaaagg ccccaaagca atgactgatg
     2701 tatctgtttt ttctttgaaa taatcagtca gaaaatcaac tgcatggagc gattctgcct
     2761 gtttggctaa gaccatcggg aaatttgtgt cccctaatcc atccaaccca tgagtgtctt
     2821 gcgcactaat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca gggatatcta
     2881 aacgatcaca gcgctccaaa catttcagca cattctgcgt accgatatct acagggacat
     2941 tcccgcaaac gacggtaata gcaactactt cgatatcagg ggatttgaca gctagtgata
     3001 aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tactttacgc attatttcca
     3061 atctcctttc tttagagata cttagttttt tattctggga agtttccgaa ccagtccaag
     3121 acgagtgtct tgtttataat gcacgaaatt agtatactgg cttttattgt gaatgtaaat
     3181 gctctcttac aagtaaatct attgagaaga actttaagta aatgatacaa tgaaaaagaa
     3241 atggaattta cgtgtgccga agaaaaagaa tcctattgat cggtatattg tataataaat
     3301 agattatagt ttggcagaaa gcggcgggtg tacgatggtt attttttatt tcattcttat
     3361 tctgatattt ataggaatca gttaccattt tctttcttat ttttatgcaa agaaattagt
     3421 aagagcgggg gtacagagag gctatgactg gtatgaagaa actgggcacc gcttgatgga
     3481 tcctgctgca gtcacgtcag tagaaaaaaa gcgacaaaaa ttagaagaag agtgtgaaag
     3541 cttctgggaa caaggaatac cgaaaacgat caaaagttat gacgggttga aactagctgg
     3601 acagatgttc ctccaaccaa ctcagcagaa taaatgggtc atttgtgtac atgattatcg
     3661 aagtacaggg aaaagagata tgtctcatat aggtaaaaga tatgcagaga aaggattcaa
     3721 cgttcttata cccgatttac gtgcgcatgg agaaagtgaa ggagaaatca tcggaatggg
     3781 atggctagac cggctagatt taattgcatg gatccaactc atcttagatg aacaaccaga
     3841 tgcatccatt attttgcacg gcagttcgat gggcgcttct actattatga tggcaagtgg
     3901 tgaaaagctt ccatctgcag tcaaaggatt tatcttagac agcggatatg tttctgttta
     3961 tgcggaattt cggtatatgt taagtaaaat cacggtcttt cctaaaaaaa tgatcatgag
     4021 atatgcgaat cactatgctc agaaatacgc aggttattct ttaaagcaag catcagctac
     4081 aagacagctt ggtagtaacc atttgccttt gttagtcatc catggtgaaa gagatcattt
     4141 tgttcctaca gaagcagcat acacgattca aaacgctacg gcaggggata aagcattact
     4201 gcttgttcca gaagcagaac atttggaagc atcgatgaaa gatccgaaaa cctattggac
     4261 agtgattttt tcatttattg aacaaagaat aacgatggat tgattttctc ttcctttttg
     4321 taagcaacac gaggataaat aattgtttat agaccatgag actgttaaaa taaggtagga
     4381 ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagag gaaaaaaatc
     4441 gtcttgcttt attcaatgat gagcaacaag aaatcggaga aatgacatgg tcggatgcgg
     4501 ggccagacat catgatcatt gaccatacat ttgtcgatcc agcatatcgt ggtcaaaaat
     4561 tagctgaaaa gctagtgtat actggagtag aactagcaag acgagagggg aaaaagatta
     4621 tcccttcatg cccttacgca aaaaaagagt ttgagaagaa accagagtat catgacgtct
     4681 tacgccaatc ataataataa cagctgaaag atttgcactt tcagctgttt ttccatttat
     4741 atagagttgt cacggagttt tatctatgat aaagttaaag agacgatatc ttttgggaag
     4801 gaggctgcta tatgtataat ttaattttgg gaatcgtgat cgttttatcg attgttatgg
     4861 tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca tttaccggcg
     4921 gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc atgcagcgat
     4981 caactgcagt catgggagct gtctggatga tcttattatt tgtcctagca tttttatctt
     5041 cgaagtagtc gaataagcct ctcgcacaac ggcgggcggc tttttttatt gcaaagagaa
     5101 cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag gtgaaacgag
     5161 tgaaacaatt accaaaaccc ctttatgcaa aacatggaaa acgagcagtt ttgctgcttc
     5221 atgcgtactc aggaagtcca aatgatgtcc ggatgctcgc acggttttta gaaaaagcag
     5281 attatacagt ctatgcgccg ctatttaaag gacatggaac gatggagcca tatgatatct
     5341 tgcaagaaaa agcagaaagc tggtgggcag atactaaaaa agctatccat tttttacagt
     5401 ctgaacagtt ttctgatatt gccgtattgg gattatcgat gggtggcatc tttgctgtac
     5461 gggcactaga agaagaatca gtaattggcg gcggtttttt ctgttcgccc ctgtctcctg
     5521 tagaaacaaa cgtgcctgaa aattttgaaa aatatgtacg tcaagtgctg aagatagcag
     5581 gcaaatcaga aaaagaaatc aatgaaaaag ctgtgtctta ccgctcgtta gctgaacatc
     5641 aattaatgga tatacaagac caagcagcaa tcgcagaaag tagactatca gatatccaac
     5701 agccaatatt tttagctcaa gctgggaaag atgaaatgat cgacccaaat ggtgtatttg
     5761 aaacggcacg caaattgagc cgccaacgag tgacacttca atggtatcct gaaagcggtc
     5821 atgtgattac agttggcaca gcaagacgcg aattagaaaa agacgtgttg gaatttttag
     5881 aaaaattacc ttggaatgag gaataaatat gacaaaagaa acaataaaag agaaaattct
     5941 ctttttcatg gaaagcagtc gcaaacgtag tttttctatg gaagaaatcg ctgaaggtct
     6001 aggctttcaa aaaagcgatg attttaaatt actagtacaa acgattgctc aaatggaaag
     6061 agaacagagt atcgttttca ataaaaaagg aaaagtcaaa cttcctttaa aaccgatctt
     6121 ggtcgaaggg acatttcggg caaatgaacg aggatttgga tttgtaacga ttgatcccga
     6181 agaagatgat atctatatcc cgaaagaagc gactggcttt gcaatggatg gggataccgt
     6241 agcaattgat atcatgaagc gagcaaatac agatatggat cgcggagcag agggaaaagt
     6301 agtggagatt cgagaccgag cgactactca gttagccgga gaatttgttg cttattcaga
     6361 cgaagagata caggaaacgg atctttatgg tgtggtcatc ccgaaagata gaaagcttaa
     6421 ccaattcaaa gtgtatgcag cggcagaggg agttcgtcca gttgacggaa gtattgtatt
     6481 gatcgaactg acccattacc ctgaaaaagg gtatgcaact agtttagaag ggataatcaa
     6541 gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcgattg tcgtggctaa
     6601 cggtattcct acaaaatttc ctgatgatgt gttatctgag gcagatgaag ttccagacca
     6661 gatcagcgaa aaggatattg ttggacgcag agatttaaga gatcagctca tcgttacgat
     6721 tgatggagaa gatgcaaaag atctagatga tgcagtgacg gtcaaaaaat tagaaaatgg
     6781 taattatttt cttggagtac acattgcaga tgtatcttac tatgtgacgc aaggcagtca
     6841 actggacaga gaagcatacg aaagaggaac aagtgtctat ttgaccgatc gagtgattcc
     6901 gatgatccca caacgtttat ccaatggtat ctgttcattg aatccccatg ttccacgact
     6961 caccatgagt tgtgaaatgg agatcgatcc aaatggacaa gttgtctctc atgagatttt
     7021 tccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc aaatcttaga
     7081 agatcaagat gaagaaacga tgcaacgtta tgctcagctc gtgcctatgt tccaagaaat
     7141 gggtgaattg catcgaatcc ttgaaatgat gcgaatcaaa cgtggagcga tttcttttga
     7201 ggatcgtgaa gcgaagatcc ttgttgatgc tgaaggacac ccaaatgata tccagctaag
     7261 aagcagaggg ataggtgaac gcttgatcga atcctttatg cttgctgcca acgaaacggt
     7321 agctgaacat tataataaat taaatcttcc gtttatttat cgaatccatg aacagccaaa
     7381 agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc tggtccgagg
     7441 aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag tagaagacaa
     7501 accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag cacgctattc
     7561 agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact ttacatctcc
     7621 tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca gtgaagataa
     7681 aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag atcatagttc
     7741 taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga agaaagcaga
     7801 attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg ttgtgaaatt
     7861 cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca atgaattgaa
     7921 acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa gaactggtct
     7981 gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc cagatgaacg
     8041 agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac gtccaaaagc
     8101 acgcaatgga aaatctggaa aacgagaagg aaaacaagca ggcaaacgct cagaaaaaaa
     8161 tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga aaaaagggaa
     8221 aaagccattt tacaaaggtg tcaaaaagaa gaaaaagaaa aaataaaatg agttggagga
     8281 aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag ctcgccatga
     8341 ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg agatcaagtc
     8401 gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca acggggaagc
     8461 tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta atcatgatcc
     8521 gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg aaaacgaatt
     8581 gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg gctatgccaa
     8641 ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag atttgaaacg
     8701 aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat aatttcatta
     8761 tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg ttagcattta
     8821 atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg tgcatgggga
     8881 aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga aaatgttttg
     8941 aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg aaaaaaccag
     9001 atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat ggattggatg
     9061 aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt tgtataatgg
     9121 tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga aatcttaagt
     9181 tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt cgaggaatcg
     9241 taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca tttacattgt
     9301 ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc ttacctaacg
     9361 aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact ttggcattta
     9421 taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa ggatatttcg
     9481 taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt gaagaattta
     9541 caaacttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg gaagttttgc
     9601 taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca ttagcaattc
     9661 cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa gcatttgtct
     9721 ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa taatactcgg
     9781 ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa ttatatcgca
     9841 gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg tcaagttatc
     9901 tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt ccgtacaaca
     9961 atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt tatcgccttg
    10021 attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat cgtttgttac
    10081 tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc tatgctgaat
    10141 caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt tgtcagcggc
    10201 tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat cagcgatatt
    10261 ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca atctcgcatc
    10321 aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg ttctgatgca
    10381 gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa tattttgaag
    10441 gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat cactgtagaa
    10501 cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct tcaaatcgcg
    10561 gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat caatcaatac
    10621 attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa gacgttacgg
    10681 gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct atcaagatct
    10741 cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt taagtgatgt
    10801 tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg gttatgatgg
    10861 aatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg accttttgtt
    10921 catgattgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat tgggaagtgt
    10981 cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga atgttgcaaa
    11041 aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta ttattggtgg
    11101 cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc aattagaaaa
    11161 aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg gccatcaaag
    11221 cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac gtgctttcag
    11281 tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca catggtttag
    11341 aaaacgcgat gagtggtgag ttgcttgaat tttcaaatgg ctcatacgga atggcgcaaa
    11401 acttagaatc aaatgatgta ggtatcatta tccttggcga tttcgagtcc attcgtgaag
    11461 gagataaagt aaaacgaaca ggaaagatca tggaagttcc agttggagag gccttgatcg
    11521 gacgggtagt taatccgcta ggtcagccaa tcgacggact aggtgaaatc gttactgaca
    11581 aagctcgtcc agtagaagca atggcaccag gtgttatgca acgtaaatct gttaacgaac
    11641 caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc ggacaacgtg
    11701 aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgatcgat acgatcatca
    11761 accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa gattctacag
    11821 ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca atcgttgtga
    11881 atgccggtgc gtctcaacca gcgccgttgc tttatatcgc accatatgct ggtactgcaa
    11941 tgggtgaaga gttcatgtac aacggtaaac atgtcttgat catttttgat gatctttcaa
    12001 aacaagctgt tgcttatcgt gaactttcct tgctacttcg ccgtccacca ggtcgtgaag
    12061 cttatccagg ggatgttttc tacttacact cacgcttatt ggaacgtgca gcaaaattaa
    12121 gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca caagctggag
    12181 atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa atcttcttag
    12241 aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta tctgtatctc
    12301 gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact ttacgcctag
    12361 acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac ttggatgcag
    12421 caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa caaaaattgc
    12481 atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca catggattct
    12541 tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc gaatatctgg
    12601 ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt ccagaagaag
    12661 aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca aaaggaagta
    12721 attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt gagatgaatg
    12781 ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac gagccaaatc
    12841 acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc ttcttcacag
    12901 aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac tgctactcag
    12961 ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag catgctgctc
    13021 agccgtcctg ttaagaaaac tggttatatc gtcatcactg cagatggcgg tttagtcggt
    13081 ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga ccaccaatca
    13141 cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt taaagcacgc
    13201 ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt tgatgaagtc
    13261 cgcaaaatcg taaatatggc aacaactatg ttccaaaacg aagtattcga tgaattatac
    13321 gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga aaaaatgctg
    13381 ccaatctcag acttagatcc agaagaagca actactttcg aacaagaata tatttttgaa
    13441 ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag tttgatctac
    13501 ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc aatgaaaaca
    13561 gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttataa ccgtgcaaga
    13621 caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc actagaatag
    13681 tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa tcggtcccgt
    13741 tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg cattagttgt
    13801 ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag aattagggga
    13861 cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag gaatggaagt
    13921 cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag gtcgcgtatt
    13981 caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg cggaaagaag
    14041 cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag aaattttgga
    14101 aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga aagtcggact
    14161 attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc ataatatcgc
    14221 ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc gtgaaggtaa
    14281 tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca tggtgttcgg
    14341 acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt tgacgattgc
    14401 ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca acattttccg
    14461 tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt ctgcggttgg
    14521 ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca catctacgaa
    14581 aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact acactgaccc
    14641 ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac gtaaattaac
    14701 agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg ctttagcacc
    14761 tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg tattgcaacg
    14821 atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat ccgatcaaga
    14881 aaaagtgtta gtttctcgtg ctcgcagagt ccaattcttc ttgtcacaaa actttaacgt
    14941 ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa cagtgaaagg
    15001 attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt tccgaagtgt
    15061 tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga aaggggtgcc
    15121 ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc tatgatcacc
    15181 atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg cctaaacatg
    15241 caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca gattcagata
    15301 cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat aatgttgttt
    15361 caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt gcggaacgtg
    15421 caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat acgaacgaat
    15481 tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta tctaagcact
    15541 cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag gttatgacag
    15601 aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    15661 aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatca gaggttgtga
    15721 aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct catattttgc
    15781 cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg gaaaaaggga
    15841 ctgcgacaag acttttgtct cagtcccttt ttttgcatat ttttagcatt tatttcctaa
    15901 aaaatgtaag gggattccta tatttttaag tatacttgtt tttcctttta tacattttat
    15961 ggtattatca ttttattagt tattataaaa caatcgttag aaaggacgtc attatgcaag
    16021 tatacggaat tgatgcggtg cttcgaattg tgagtcattt ggcgtttatc tacttagcct
    16081 tctggtcttt acgttcgtta cgcattgaaa cttttttcag accaatgcat aatacgcaaa
    16141 ttagaatggc aatcgtactt ttttcaattt ttttagggta cactgcaagt aattttttct
    16201 tagaagttat cgctttatgc cgaaatcttt tcgttagcct gtaaatttta aaaaaaggta
    16261 ctattttaaa agcaaggatg ttataataga aaagattttg agtaagtgta tgctcattgg
    16321 aattttttgg agggaacaac atggaagaga tcatcgtaag aggtggcaat caattaaatg
    16381 gaacagtaag aattgaagga gctaaaaatg ctgttttgcc tattttagct gctagtttgt
    16441 tagcagaaga aggtattacg acattagaca acgttccgat tctttctgat gtcttcacta
    16501 tgaatcaagt gattcgtcac ttaaacgtag atgtagattt tgatgaacaa aagaatcaag
    16561 taacaatcga cgcttcaaga caattagaaa tcgaagcgcc ttatgagtat gttagtcaaa
    16621 tgcgtgcttc aatcgttgtg atgggtccgc tacttgccag aaatggacat gcaaaagtag
    16681 ctatgcctgg tggatgtgcg attggtaaac gaccaatcga tttgcattta aaaggatttc
    16741 aagctttagg agcaaaaatt attcaaaaaa acggctatat cgaagcaatt gcggatgaat
    16801 tgatcggtaa cacgatttat ttagatttcc ctagtgtagg tgctactcaa aatatcatga
    16861 tggctgccgt aaaagcaaaa ggaacaacca tcattgaaaa cgtagctcgc gaaccagaaa
    16921 tcgttgattt agcaaacatt ttgaataaaa tgggcgctca agtctatgga gctggtactg
    16981 aaacaatgcg tattgaaggc gtggatcatt tacatgcagt caaccactca atcgtacaag
    17041 atcgtatcga agcaggtacc tttatggttg ctgctgcaat gacacaaggc aatgtgctga
    17101 ttgcagatgc tatctctgaa cataaccgcc cattgatttc taaattgatc gaaatgggtg
    17161 cagaaatcat tgaagaagaa ggcggagtcc gtgtcatcgg accaaaacat atcttaccta
    17221 cagatgtaaa aacgatgcct catcctggtt tccctacaga tatgcaagct caaatgacag
    17281 ctatccaatt agttgcagaa ggtacaagtg tggtgactga aacagtgttt gaaaatcgtt
    17341 tccaacattt agaagagatg cgccgtatga acgctcatgt caaaatcgac ggaaatgtag
    17401 caatcatgga tggaaaccat gaattgcaag gtgcagaagt atatgcaaca gacttgcggg
    17461 cagcagcagc attagtttta gctggcttga aagcaaatgg aatcacacgt gtgcgtaact
    17521 tgaattatct agaccgtgga tattataatt tccatatcaa actacagcaa ttaggtgcag
    17581 acgtcgaacg tgtagacatg gatcaaactt ctgctgaaaa aacagcacaa acgattgctt
    17641 aaggaagtgg ctgaatgagt aaccaacgat atatcttatt aactttgata aagattttag
    17701 tggtgatctt attgcttatc cttctttttg tggccggaac gatgatcgga tatggtgtca
    17761 tcggtggcgg caatccattt aaagtattcc agccaagttt gtggattcat attcgagact
    17821 tttttcattg ataaataaga atcacagtgg ttctagtcga tatgaagtga gccatgaagc
    17881 agaacatgat aaaagaaaac agaagggaac gttgacgtta gtcgcgatcc ctttttttag
    17941 taaaagaaaa gaaggaatct aattgatgaa gaagatattg atttatttag ttagaggata
    18001 tcagcgcttt atttcgcctg catttccacc tagctgccgt tattacccaa cctgttccaa
    18061 ttatatgata aaagcaattc aaacccatgg tgcgctaaaa gggacggtaa tggggactgc
    18121 acggatctta cgctgtcatc cttttgtcaa aggcggtatc gattatgtac cattgacatt
    18181 ccgattaacg cgaaataaga aagataaaaa agaaccagat tataaaaagg ttgtgaaaaa
    18241 tacgggtagc actgagcgat aaagaaaaag aggaaattta ttgtcaaagt aacaactccg
    18301 caactactta tatgggggac aaaaaatgat cgaagaacta aacaagaaag atttaccatg
    18361 gtccttgtta ttagatgcag atcctgataa agaaaaagtt caagtatatg tttccagagg
    18421 gagtggctgg atatggaaag aaaaagaaaa aacaataggt gtactgatct atgtggcaag
    18481 ggaaaaagaa tacgaaatcg tcaatgtagc agtaacccca acgtatcaag gaaaggggat
    18541 tggcggaaaa ttacttgaga cagcttttca gaagctttca cagctagctt caaaccaaac
    18601 tcggatcatt atacgtactg gcactactag tagcgctgcg ttacacctct atcaaaaaat
    18661 gggctatgtg gagattggtc gagtcaaaga ctattttatc catcattact ctgagcccat
    18721 ttttgaaaat ggagatcaat taagggatca agtgatttta gcaattcact tacctaaaaa
    18781 tagctttaac taataaactt tcaattcgag aaaatgcttt caaatgctaa atagatacat
    18841 tacaatagct ttagcaaaca aaaaggagaa tatttttatg ggaatattag tttttgattt
    18901 tggtggatcg gctgtcaaat atggttgttg ggatggcaaa gagattaaag gtaagggaca
    18961 atttgcaacg cctgaatctt gggaagaaat gaaaacgcaa ttatttcaag tttataaaaa
    19021 aatactcgtt tcttttgaag tcgaaggaat tggaatcagc tctcctggag tagtgaataa
    19081 tgagaaacag gtgattgagg gaatcagtgc gattccttat attcatggat ttaatatttt
    19141 ccgagatttg gaaactctgt ttcaactccc agtaacgatt gaaaatgacg ccaactgtgc
    19201 cggaatggct gaattttatg aaggtgctgc ccgtgattat caagatgttg cttttgttgt
    19261 ggcaggaact gggattggcg gggcgttgtt cacgaaagga caaataaata aaggggcaca
    19321 tctttacggt ggagaatttg ggttgatgtt cttagatggc gataagacat tcagtaaact
    19381 tggtacagcg gtacagatgg cttggcgtta ttgtgaacgc aaaggtctcg ataaaaatgc
    19441 ttataccgga aaagatgtct ttgaattagc agaaactgga gatgcaatag caaaagaaga
    19501 agttgagagt ttttatacgt atcttacaaa gggattattc agtattcaat tttcgtttga
    19561 tccagaagtg atcgtattag gcggcggggt ttctgcaaaa gaagggctca ttgatgagat
    19621 caaaagccga atgaagaagc tgacagaaca attcgatctg catgatttcg aaccgcagat
    19681 tttattatgt gagtacagaa acgatgctaa tcttgtaggt gcagcagcaa actatctcgc
    19741 tgtacgtcaa ggagagaatt aagagcttta gagaaggtgc agcttgcata aaagaagaga
    19801 atgaaatgaa taggagattt ttacctaagg aatgttttat ttgctgcagt atgataaaat
    19861 atctgagagg tgaaaatgat gtcaaaaaaa aataaagata ttgaagtgcg tatagaagaa
    19921 gtgaaaaaag atatcaaagg taagaattat gaagtcactc agttatttat agggaaaaaa
    19981 atgattggtg agatcttagc ctatgggcca aaagaatatg aaattttctt tggtgaagaa
    20041 gattttggaa aagaaaaaag tcttgaaaat gcgatagaaa cagtgattcg acactggaat
    20101 ctgcacgaat aaaaaaatat caaaaaaact ttcaataaag ggttgcaaaa atctggaaaa
    20161 ttaggtatta taactaagtc aggttgatac aaacctttcg gaggagtagc gaagtggcta
    20221 aacgcgacgg actgtaaatc cgttccttag ggttcagtgg ttcgaatcca ctctcctcca
    20281 tttcattggg gtatagccaa gcggtaaggc aagggacttt gactccctca tgcgttggtt
    20341 cgaatccagc taccccagta taccacaatc gtaagctctc gttagttcga gagctctttt
    20401 ttttgctttg ctaccttgtt ccttgaagat tctgctgaat taagatagag ttaagttaac
    20461 tacaagaatt ggagggatcc acatgaaatg gaaagaaaaa gtagacgaag cagctgaaaa
    20521 gttatatgat ttggttaaat cagaaaaata caatattgaa gtgaatattc cgaaaaaagg
    20581 cggaaaagcg gtccaggtaa aatcaaaacg cccaacgaat catacaaaaa aatggatggc
    20641 taaaaaccgc taataataac aaaagatcca taagtcggca ttttcatcga ttttagggtc
    20701 ttttgttatt ttctaggttg aacatatcta aaaaattgtt tctgacaata ggagtaggga
    20761 gaacgatgtg tgtctaaatc ctctgttgat ggatagaaac ctttatcttt ttcagttgtg
    20821 aatcaggtat ataccagtta taatagaaga aacgaaagga cgggtgtacg ttatgtcgaa
    20881 tcaattacct gtatatatac agattcatga ccaattaaaa aatgaaatcg aacaaggtat
    20941 atggaaagtc ggagaccgtc ttccttcaga aagagaatta gcagtgaaat tcaatgtcag
    21001 ccgtatgacg ttgagacaag caattcaaaa cttggcagat gaaggaatct tagaaagaaa
    21061 gatcggttcg ggtacgtatg ttgcgcgaga aaaagtacaa gaaaaaatgt ctggtgcaac
    21121 aagttttaca gaaattatgg aatcacaaaa tcgagtaccg tctagtcgga caatctctta
    21181 tttcttgact tcgccaagct caagtgaaat ggaaaaacta aacttgagta aagatgacac
    21241 gatcttgcga atggaacgta ttcgtttcgc agatgatgtc cctatttgtt ttgaagttgc
    21301 cagtgttcca caaaagctga tcgatggcta cagcaaagca gagattactg aatcgttgta
    21361 tcgcacgcta gaagaaaaag gcgggaaaaa gatcggtcca gcgaatcaaa cgattactgc
    21421 tgtgttggct tctgagaaaa ttgctgacta tttagaggta aaacgaggag atgcagttct
    21481 tcgtttgcgc cagatttctt attttgaaga cggtacgccg tttgagtatg tgcgtacgca
    21541 atacgtagga aatcgttttg agttttattt ggaaaaataa aaaacaggtg atccctgatt
    21601 ccttattaga aataaaagag aatcagaatc acctgttttt atatatgatc agttatcagt
    21661 tgctcgtgat aagaaacaga accctcttca aattggactt gtccgttcag cagctcaatg
    21721 atggattctt taaagtggtc agcacttttt tcatctacag cacagatgac gctaacttga
    21781 tcagtatatt ggatttcttt tataaaaaaa tgttggagtt caagataatt ctgtaatttt
    21841 cctagttgcg gatacgtgag atctcctgtt gcaaggtacc ttctacgatt ccaatgtcag
    21901 acaatgcttg agagaccgca tgagtgtatg ctcgtattaa tccgcctgtg ccgagtttcg
    21961 ttcctccaaa atatcgagtg actactgcag ctacattgat cagttgattt tttttgagga
    22021 cttccaacat cggtacgccc gccgttccac ttggttctcc atcatcgctg cttcgctgga
    22081 tttcattttt ttctccaatg atgaaggcgc tgcagttatg gttcgctttc caatgttctt
    22141 tttttatttc ttgaatgaaa ctttttgctt ggtcctctga ttctattcgt ctaagagaac
    22201 agataaagcg tgattttttg atctcaatct cactttgtcc attttctttt attgtacgat
    22261 agcgctcgat catcttgtca cctctttttt tcattttctt tattcagtat aaataaagtt
    22321 ctaggttgtt tcaacactgt aacggttgcg caattgctgt agtatgcgat aataaagaca
    22381 aaggcaggtg gatctgggga tgcaggaacg aatgaaaaag tacgatgcaa ttacgcatta
    22441 tttgaagaac aatggcggct ctcaagtaac gttgaccttt acccaatttg acgaactgct
    22501 gtttcccagt aacgggcttc ccaaaacagc aagggaaagt acagattggt gggcaaatga
    22561 ttataaacac cctgaaaaag gtgcctatgg ctggataaat gcaggctatg aagtagttgt
    22621 catcaatttg gacaaagaat atgtcgtgtt caataaatta gtgaaatcta gttggctatt
    22681 tgattaaata aaccgcgttt agaaaacgtt gacaaatttc tttcttttta tataatagag
    22741 taacagacgc gaagaaaacg tttgcatagt ttgttttaaa aaggagagaa aagtatggct
    22801 aaaaaaacaa ttatgcttgt atgttcggct ggtatgagca caagtttact agtaacaaaa
    22861 atgcaaaaag ctgctgaagc aaaaggaatc gactcggata ttttcgctgt gtccgcttct
    22921 gacgcagata acaatcttgc aaataaagac gtggatgtgt tgttattagg cccacaagtt
    22981 cgctttatga aagctgattt tgagaaacgt ctggaaccaa aaggcattcc tttagatgtc
    23041 atcaatatgg ctgattatgg tatgatgaat ggcgaaaaag tgttagatca agcaatcacg
    23101 ctcataggtg aataaataga acagtataac tatcgaacaa gcgatccaaa aaatagatcg
    23161 cttgtttttt cgtatatttt tttagggcga agaatatgaa tcatttttgg ggtagaaggc
    23221 ttctgacgag agatattgaa gcaatagact gtgaggaggt aaatttacca aattatgaga
    23281 agatcgcagc agtggaacgt ttgggcaact ggattctttg tcatcgctgt ggaatgagga
    23341 cgtccgtcat tgatggacag cttgcagatt acggttattt ttgtacacat tgtctatatt
    23401 tagggcgatg cgacactcaa caagatttat atttgtttga tcagcctgaa gcaaaacctc
    23461 gagaagtcgt ctttttttgg acaggacagc ttactgaaaa acaaacggaa atctcaaaga
    23521 gaatactttg ccattcagaa aaaagacatc atcttatttg ggctgtaaca ggtgcaggga
    23581 aaacagagat gctttatcct attttagtgg aaactttaaa agctggagga agagtagcga
    23641 tttgtacacc gcgagtcgat gtttgtaatg aattgttttt acgctatcgt tatgtttttc
    23701 cagaagaaaa aatcacgttg ctccatggga atacagcaac ggcttatacc tttacttctt
    23761 ttgttatctg tacgattcat cagctatacc gtttttatcg gtcctttgat ttaatagtga
    23821 tcgatgagat cgatgcgttt ccttataacg gggatgcagg attagctcat gctgtgcaaa
    23881 cagcgattaa accagacgga cgattcatct acttaagtgc cacacctgat aaaaagttac
    23941 taaaggaaat caagcagacg tttgacatcc acaaacttgc tttacgcttt catcggcgtt
    24001 tgcttcctga acccgtcttg gttttcatga ataattggcg acaaaaatgc aagaacaaga
    24061 agaagataaa atctttggtg cggatcattc atcaattaat taaagaaaac catatccttt
    24121 tattttgtcc aagtatcacg atgatgcatc gtttgaaagc cacacttcaa gaggctcttc
    24181 cgagatatga gatcacagat gtttcttcaa gagacaatca gcgggcggaa aaagtccaga
    24241 aaatgcgaga agcaaaatat gacatactgt taaccacgat gatccttgaa cgtggagtca
    24301 catttgaaag aatatccgtt atcgttttag gagcggatca tccggttttt actaaatctt
    24361 ctttggtaca aatcgcagga agagccgatc gtaaagggcc ttatacgaac agtcaagtat
    24421 actttttcta tgaagaaaaa actgcagcta ttcgaaaagt ttgtcaggaa atcaaagaaa
    24481 tgaatgaaga agggaagaag atgctgtgag gtgcagttgc tgcagtaaag atatcttcca
    24541 aaatttaaca ttcagcgaga ttattggtgt ttcgaaatac agattacctc agacttgttc
    24601 agtttgtcgc gcacaatttc atttattgga aacaagaggc tgttctggct gtcaaaaacc
    24661 aatccatttc aatctagaga gtacgaaaaa gaaagtaaag tctattcccg tttactgcag
    24721 tgactgtcaa gaatggaaga agaaatatcc gacctatgat tttcagcacc gtgccttttt
    24781 tgtgtacgat gaaaatatga aggcctggct caatcaatac aagtttatgg gagatatccg
    24841 actagcaggc acctttgcat atagatggac gcagataaaa aaagaatacc cgacatacat
    24901 ctattgtcca atacctttat ctgaaaaaag gcgaacagaa agaggattca atcaagtaag
    24961 cgagatgttg aaagcagcaa gaattccttt tcagatgcta ctcaaaagag agaagcatct
    25021 gctggctcaa gcaaaaaaca cgcgaaagga aagattggag atgcctcaac cttttgaact
    25081 tgcagtcacc ttttctaaga tacaaaataa aaaaatattg attattgatg atgtctatac
    25141 aacaggacag actcttttcc acgcagcaga ttgtttgcag gtcgcacagc cgaaaatcat
    25201 ccgaaccatt tctttggcga gatgaattag atgtggaaat ttgatagcga tttaatttgc
    25261 aaataaccta gagttcgata taatagaatt aagaagaggg aaagagtggt ccttctcctt
    25321 cttgattgtg gtagacgaaa ggggtaattt ttatgtttag atataatgta cgcggcgaaa
    25381 atatcgaagt aactgaagcg attcgcgact acgttgagaa aaaagtaggc aaactagaac
    25441 gctatttcag tgacgcaccc gatgcaacgg cgcatgtgaa cttaaaagtt tatacagaaa
    25501 aaacagcaaa agtggaagta acgatcccat taccttatct tgtattacgt gccgaagaaa
    25561 cttctccaga tttatacgca agtatcgatt tggttgttga taaattggaa cgccaaatcc
    25621 gcaaattcaa aacaaaaatt aatcgtaaat cccgtgaaac gggcatgaat actgcagatg
    25681 ctgctgcttt attcaatgaa gaggacgaaa acgaaaatga ttctgaatta gatatcgtac
    25741 gtacgaaacg cctttcattg aaaccaatgg atagtgaaga agcagtctta caaatgaaca
    25801 tgctgggaca taacttcttt atttttgaag atgcagaaac aaatggtaca agtatcgttt
    25861 accgccgtaa agatggaaaa tacggactga ttgaaacaga ctgatcagcc ttacttaaat
    25921 aacaacgaaa acttgcagca gctactgaca gctgccgcaa gtttttttga gtttcctcct
    25981 cttttagatg aaatatgaat aaattatgaa gttcttactt taatacgctt aagatatttc
    26041 aattatctat aactaatgat aaaatgaaaa gtaaagtcta catagtctgg ataatacaca
    26101 gaaggaaaag gagattacac aacgcatggc caatttgatt cgttcactta tcgaaaacga
    26161 taagaaagaa ctaaaacgtt tagatagcat tgcaaaaaaa gttgaagcat acgctgacca
    26221 aatggcagca ttaactgatg aacaattaca ggcaaaaact gctgaattca agggcagata
    26281 ccaaaaagga gaaacattag atcaattact tccggaagct tttgcagtcg tccgagaagc
    26341 agcaaaacgt gtattaggtt tatatccata ccacgtacaa ttgatgggtg gaatcgtgct
    26401 tcacgatgga aatatccctg aaatgcgtac tggtgaaggt aaaaccttga ccgcaacgat
    26461 gccagtatat ctaaatgctc tgactggcga aggggtacac gtagttaccg tcaatgaata
    26521 tttagcaacc cgtgactcga cggaaatggg cgaactttat aatttcttag gattaacagt
    26581 ggggttgaat atcaactcaa aatcttcgga tgaaaaacgc gaggcttata attgtgatat
    26641 cacttatagt acgaataatg aattaggatt tgactacttg agagacaata tggtcgttta
    26701 ccgccatcaa atggtacaac gtccgctgaa ttatgccatt gtcgatgaag ttgactcaat
    26761 cttgattgat gaagcgagaa cgccgctgat catttcaggg caagcagaaa aatccacagc
    26821 tttatataca agagcagaca acttcgtgaa acgtttgaaa gaagaagaag attataaaat
    26881 cgatgtacaa tcaaaaacaa tcagtttaac cgaagcaggg atcgaaaaag cagaagaaac
    26941 attcggtttg gataacttat acgacattga aaatactgca ttgacccatc acttggatca
    27001 agcattgaga gccaactata tcatgctttt agatatcgat tatgttgtcc aagacggaaa
    27061 agtactgatt gttgaccagt tcactggtcg gatcatggat ggacgccgtt attcagatgg
    27121 acttcaccaa gcaatcgaag ctaaagaagg cgtagagatc gaagacgaaa cgaaaaccat
    27181 ggcgacgatc actttccaga actatttccg tatgtacaag aaacttgcgg gaatgactgg
    27241 tacagctaaa acagaagaag aagaattcag agaaatttat aacatccaag tataccaaat
    27301 tccgacaaat cgtccggtta tccgtgatga tcgtccagat ttgctttacc caacattaca
    27361 aagcaagttc cacgctgtcg tgcaagatat caaagatcgc taccgcaaag gtcaacctgt
    27421 gttagtcgga actgttgcgg ttgaaacatc tgagttatta tcagatatgc tgaatcaaga
    27481 acgtattcct catgaagtgc tgaatgcgaa aaatcacttt aaagaagcag agatcatcat
    27541 gaatgctggt caaaaaggtg ctgtgacgat tgctaccaac atggctggtc ggggaacaga
    27601 tatcaagtta ggacttgggg taagagaact tggtggtcta gcagttatcg ggacagaacg
    27661 acatgaatct cgtcgtatcg ataaccagct tcgaggacgt gcaggacgtc aaggagaccc
    27721 tggtatgtca cagttctact tgtcattgga agatgatttg atgaaacgat tcggttcaga
    27781 acgaatcaaa gctttcttag atcggatgaa gatcggtgag gaagatgctg ttatccaaag
    27841 caagatgctg acgaaacaag tagaatctgc acaaaaacgt gtagaaggaa ataactatga
    27901 cacacgtaaa aacgtcttac aatacgatga tgtgatgcgt gaacaacgtg aagtaatcta
    27961 taaacagcga caagaagtaa tcatggaaga aaaaagctta tccaagccac taatgaacat
    28021 ggtcaaacgg acaatcagtc gtgtagtaga tgcgcataca caattagaga aagaaaattg
    28081 gaatttggaa agtatcatcg attttgccgg cagtactctt gtccatgaag atagcattac
    28141 tcttgcagat atcgaaggaa aaacagctca ggagatcaaa gagtatctaa acgaacgagc
    28201 acaagaagta tttaatacca aagcggctca actaaatggt ccagagcaac tgttggaatt
    28261 tgaaaaagtc gtgatcttgc gtgttgtgga ctctaaatgg acagaccata ttgatgcaat
    28321 ggatcaatta cgtcagtcaa tcggcttacg tgcttatgga cagaacaatc cattagtcga
    28381 atatcaaaca gaaggctata agatgtttga agatatggta ggcgccatcg agtatgaagt
    28441 cactcgaatc tttatgaaag ctgagatccg tcaaaacgta cagcgtgaac aggtttctca
    28501 aggaacagct tctcactcag aagacggaga tgccaaagaa aatagtaata ctagcgcgaa
    28561 aaaacagcca atccatgtga ataaggtcgg aagaaatgat ccttgtcctt gtggaagcgg
    28621 gaaaaaatat aagaactgtc atggaaaaaa tgcaaactaa tttctgacag caggattggt
    28681 gagacaaatt ctttttttaa gaattcgtct caccttcttt ctgttatttg ctgattctgt
    28741 tagactagat ataatgaaaa aagtatagtg aaagaaggaa aaaacatgga aatatccgaa
    28801 attcgaaatc tcctgacaga aatgcaggaa aaggtcacaa gtttcaggag gtctctttga
    28861 cttagatcag ctggaagaag acatcgcgga agcagaagca aaaatggctg aacctggttt
    28921 ttgggatgac agcgaagccg cacaaaaagt gatcagcgaa aataacgcta ataaagaaac
    28981 ctatgaccaa ttccattctc ttgccgaaga actagaagaa atcgaattga tggtggagat
    29041 gctgcaagaa gaacctgatg cagcaatgca agaagaagca gaaacacgta tcaatgcatt
    29101 agatgaaaaa atgaagacgt atgaattatc tatgctgtta gacggacctt atgatcgaaa
    29161 caatgcgatc gtcgaattgc atccaggggc tggcggaacg gagtcccaag actggggcag
    29221 catgctttta cgtatgtata cacgttgggc agagcagcat ggctatcaag tagaaacact
    29281 agattatcaa gcaggtgatg aagcagggat caagagtgtg accctgctga ttaaaggaca
    29341 taacgctttt ggttatctaa aatcagaaaa aggtgttcat cgccttgtac ggatttcgcc
    29401 ttttgactca gcgaaacgac gtcatacttc tttttgttcg gtagatgtga tgccagagct
    29461 tgatgaaacg atcgatatag aaatcaatcc agatgatttg aaaatcgata cgtaccgagc
    29521 aagtggtgca ggtggtcagc acatcaataa aacagaatct gctgtacgga tcactcactt
    29581 gcctacagga acagtagtag ctagtcaggc acaacgctca caattgaaaa accgtgagca
    29641 agcgatgggg atgttaaaag ctaagcttta tcaattagaa gtggaaaaga aagaacaaga
    29701 agcagctgca cttcgaggag aacaaaaaga aatcggctgg ggttcacaga tccgttctta
    29761 tgttttccat ccctattcta tggtaaaaga ccatcggaca aacgtagaga caggaaatgt
    29821 ccaagcagtt atggacggtg accttgattt atttattgac ggctatttaa aaatgcatct
    29881 acaataagaa cccattgtta aattgaaata aaattgtaag attttgcaag catgttgaaa
    29941 cattgccatg ctataatagc taaagactga aaaagatatg gagagaacgc catgattgaa
    30001 atgaaggatg taatgaagaa gtactcaaat ggtacgaccg ctatccgcaa tctctcgatt
    30061 gtcatcgaac aaggagagtt tgtctatgtg gttggtccgt ctggagccgg taaatctaca
    30121 ttcatcaaat tgatgtatcg agaagaaaaa gcttcaaaag gaactttgaa cgtcgcaggt
    30181 catgatctaa tagcaatcaa aaaccgcgaa gttccttatc tacgcagaga aatcggcgtg
    30241 gtgttccaag actataaatt attaccaaga aaaacagtat atgaaaatgt tgcgtacgct
    30301 atgcaagtta tcggcagaaa acctcgagat atcaaaaaac gagtaatgga agtgctggac
    30361 cttgttggtt tgaaacacaa agttcgagtc tttcctagcg aattatccgg tggggaacag
    30421 caacgtgtct ctattgctcg ggcaatcgtg aatacgccta aagtacttat cgcggatgaa
    30481 ccgacaggaa atctggatcc agaaaactca tgggaaatca tgaaattatt agatcggatc
    30541 aatgcccaag gaactactgt cgtgatggct acacataaca gtacgatcgt aaatacgatt
    30601 cggcaccgag taattgctat cgaaaacggc cgcattattc gagatcaagc ggaaggggaa
    30661 tatggatacg atgattagaa cctttttccg ccatttatta gaaagtatca agagtttgaa
    30721 acgtaacggc tggatgacag ttgcttcagt cagtgcggta acgatcacgt tgactttagt
    30781 cggtgtattc atggcagtta tcatgaatgc aactaaatta gctcaggata tagaaggaaa
    30841 cgttgatgta tctgtattcg tagatatcgg tacaaaagac gaagagatga aaaagctaga
    30901 aaaagaattg aatgacttag atcatgttaa aaaagttgaa ttttcaagca aagaacagga
    30961 attgaaaaaa atccaaaacg aaatgggtga ttcctggaaa ttgtttgaag gtgacaataa
    31021 cccgttatat gatgtgtatg tagtaagtgc tactgatcct tcttatacaa aacaagtagc
    31081 agaagaagca gcagatttga agaatgtttc tcgttctgat tacggtggag cttcctcaga
    31141 tcggattttc caaatcgctg gtgcagttcg gacatgggga ttagttgcag cggcattgct
    31201 gctgttcgta gcgatgtttc tcatttcgaa cacgattcgg atcacgattt tgtctagaca
    31261 gcgtgaaatt caaatcatgc gtttagtcgg tgcaaaaaat ggatatattc gctggccgtt
    31321 tttccttgaa ggtggctgga tcggtctgat cggtgcgatt cttccgattt tgatcatcac
    31381 atttggttat gcatggtttt tccaattgat caatccatca ttattacgtt cccattattc
    31441 attgattcat ccacaaaata tcgtttggaa aatcaatctt ttaatggtcg gcattggtgt
    31501 gatcattggt tcacttggct cgatcatttc tatgagacgt ttcttgaaat tctaatacca
    31561 caatataaat aaaatgagaa agttggacag tttcgttcca gctttctttt tttattggct
    31621 aggagaatag aaggaagcgg cttttttccc tttactttaa aaaacaactt gggtattctt
    31681 aagttggtag aagaagaggt gaagccgtga aaattatcgt tgacaatttt tttacgatcc
    31741 ttttgattat gaatattttg ctttccttta tcatcgtttt tcgagaaaga aaggaaactg
    31801 ctcaaacatg ggcatggctg ctcgttttga tgttcattcc agttgtagga tttattttat
    31861 atattttttt aggaagaggg atttccaaag agaaaatctt tgatttgaag atccaaggga
    31921 aaatcggtaa gaacctggaa attgaagaag acaaacaggc attgatgcga gggctttatc
    31981 ctcatccgcc tactggacaa gtagatgtga aacaattgat ctatatgctc acggtatttg
    32041 aaagtacgct ctatacgact aaaaatgaaa tcactttatt tacagatggt cgtgaaaaat
    32101 tcgatgcgct gattgaagat atcaagcagg cagaagacca tatccacttc cagtattata
    32161 tttatcgcag tgatgcatta ggtgaagaag ttcgagatgc attgacagag gcggcaagac
    32221 gaggggtaaa agtcagagtc cttcttgatg catggggttc cacacaagtt tcttccagct
    32281 tttttcagaa tttgaaaaaa gcaggcggag aagtcgcatt tttctttcct ctatttgttc
    32341 cgtatattaa tcctcggatc aattatcgca atcatcgtaa aatcgtagtt attgatggga
    32401 aaatcggata tacaggtggg ttcaatgttg gaaatgaata tctaggattg gtaaagaaat
    32461 ttggttattg gcgagacaat catttgcgta tttacggaga agcggtgtat accttgcaaa
    32521 accgtttttt gatggactgg aattctcagc atacaaaaga agtaggctat tctcccaaat
    32581 tttttccgtc cattcacact actggtgata tagctgttca aatcgtgaca agcggaccag
    32641 ataccgaaca tgaacaaatc aaaatgactt atttgaaaat gatttcgtta gccaaacggg
    32701 agatattgat ccaaacgcct tattacatac cggatggatc gattcatgaa gcactgaagt
    32761 tggctcttct ttcaggcgta aaagttcata tccaaatacc taacaaacca gatcatcttc
    32821 tcgtttactg ggcaacgtat tctttcgctg cagagctgat cgagtatgga gcaaggatcg
    32881 aaacatatga gaatggattt attcatgcaa agacgatgat tatcgatggt ggcattgttt
    32941 cagttggctc agcaaatatc gatgttcgtt catttcgctt ggactttgag gtcaatacac
    33001 tggtttatga tgaacgaatg gcttcacgtg taagaaaagc tttttttgaa gactcgaaaa
    33061 tttctacgca tctcacaaaa gaaatgtatg aaaaccgagg aataatcatc aagatgaaag
    33121 aaggattagc aagattgatt tcgccgctcc tttaaaacat aaaaaggaca tgagaaccat
    33181 caacagatgg agatctcgtg tccttttcta acggggccat ttctgagagt acgtcagaaa
    33241 tggagaaagc gtctcgcatg agcgaaatta actttggggt atctcttgtt ttttggggaa
    33301 agcaagaaat aagctaatat atgctattaa aagagacaca tttcaaagcc agtatagcat
    33361 agaaactttt aatgagatag ttttttttac aattatgaga atttattttg ttatttaatg
    33421 attttgattc gttttcatca agtaatgaaa atgataaaat aaacatactt taggattaaa
    33481 ggagatttct aatgaaaaaa cttaaattat tcttaccaat aataggatta gccattcttt
    33541 tatcaggctg ttcaaagtgg attgatcgag gagaatccat tacggctgtt ggctcttctg
    33601 ctttacagcc tttagtagaa acagttgctg aaacttacca gacagaaaat cctggaaaat
    33661 tcatcaatgt acaagggggc ggaagcggaa ctggtttgtc tcaagtacag tcaggagcag
    33721 tcgacatcgg aaactctgat ttgtttgcgg aagaaaaatc ggggatcgat gcctcagcat
    33781 tagtcgatca tcgagtagca gttgttggga tcactcctat tgtgaacaag gatattggcg
    33841 taaaagacat cagtatggaa aacctgaaaa aactatttac aggcgagatc aaaaactgga
    33901 gtgaagtcgg tggaaaagat cagccagtag ttatattgaa ccgcgcttca ggaagcggaa
    33961 cccgtagcac gtttgaaaaa tgggtattgg acggagaaac agctgttcag gcacaagaac
    34021 aagattcaag cggaatggtg cgccagatcg ttgccgacac acccggtgca atcagctatg
    34081 ttgctttttc ttatgtttcc gatgatgtag atacgttgaa tgttaacggt gtagaaccga
    34141 cagatgaaaa tgtgacaacg aacaaatgga cgatttgggc atatgagcat atgtacacaa
    34201 gagatcatcc aaaaaaactg acaaaagaat ttttagacta tatcttatct gatgaagtac
    34261 aagataacat cgttggagaa ctaggttata tccctgtttc tcatatgaaa gtcgaacgtg
    34321 attggcaagg aaatatcgtt aaagagtaat ctttacacaa aatttacaaa cttagctaaa
    34381 cttttacatg ttattaaaac caaattgatg ttcaatttgt acactgagtg agtatgttaa
    34441 acctgattaa gggggagtca tctgtggaag atgtgaaaaa agtattatta acaaaatcaa
    34501 aacgttcaaa aatggaacag cgtggaaaat tcatcagttt tttatgtatc gcgctgatcg
    34561 tgttggttgt attatctatt ttttattttg tagctagtaa aggattggcc acctttttta
    34621 ctgataagat caatgtattt gattttctgt ttggtacaga ttggaatcca agccaaatcg
    34681 gagcaaatgg gaaaccaatg gtaggggcat tgcctatgat cacaggttcg tttattgtga
    34741 cctttttatc tgctatcgtt gccacgccat ttgccattgg tgcagcagta ttcatgacgg
    34801 agatctctcc taaaaacgga acgaagatct tacagccggt catcgagctg cttgtaggta
    34861 ttccttctgt cgtttatggt tttatcggtt tatcagttat cgtgcctttt gtcagaagta
    34921 tttttggcgg gacagggttt ggtatcttag ctggtacatt tgtgttgttc gttatgatct
    34981 tgccgactgt cacaacgatg actgttgatg cattaaaggc agtgccacgg cactatcggg
    35041 aagcttcatt agcgcttggt gccacaagat ggcaaacaat ctacaaagtc gtgctacgag
    35101 cagccgtgcc cgggatttta acagcagtcg tttttggtat ggctcgtgcg tttggtgaag
    35161 ccttagctat ccaaatggtc atcggtaatg cagcattgat gccaacagga cttactactc
    35221 ctgcttctac attgacgtcc atcttaacaa tgggaatcgg aaatacaatc atgggaacag
    35281 ttgaaaataa tgtgttatgg tcattagcgc tgatcttgct attgatgtca ttattcttta
    35341 atatcgtgat ccgtatgatt gggaagaaag gagagctgaa ataatgaacg caaaacgctc
    35401 agataaaatt gctacaggtg ttttatatat agtttcgggg atcatcgtat tgattttagc
    35461 agctttacta ctttatatct tagttcgggg aataccgcat atttcatggt ccttcttgac
    35521 cgaaccttcc aaagcctacc aagaaggcgg tggaatcggg atccagctat ttaactcgat
    35581 ttatttgctt ttgatcacga tgatcatcag ttttcctatt tctttaggtg cgggtatcta
    35641 tctttcagaa tatgctggaa aaaactggct aacagatgtg attcgcacgt cgattgaaat
    35701 cttgagctcg ttgccttctg tcgttgtcgg tttattcggt ttcttggttt tcgttattca
    35761 attccagtat ggtttctcga ttatttctgg tgcattggct ttgactgtat tcaacttgcc
    35821 tcttttgact agaacagttg aggagtcttt acaagcggtc cattacacac aacgagaagc
    35881 aggattggcc ttaggtttgt cacgctggga aacagtgata aaagtggtgg ttccagaagc
    35941 gacaccaggg attttgactg gtgtgatcct aagttctgga agaatttttg gtgaagcagc
    36001 tgcattgatc tatacggctg gacaaagtgc acctgcgtta gattttacga actggaatcc
    36061 attaagtgtt tcaagcccga tcagcatttt ccgtcaagct gaaacattag ctgtccatat
    36121 ttggaagatc aatacagaag gcacgatgcc tgatggtgtt tctgtttcgg ctggcgcttc
    36181 tgccgtgttg atcattgctg ttttactgtt caacttcggc gctcgtgcga ttggcaaacg
    36241 attataccga aaaatgactt cagcctaagg agaagaagca aaatgaaaaa atataactgg
    36301 aatgaaacaa atgtcatcac catgccaaaa gagaaagaag tagcactata tacggatgat
    36361 ctacatgtct ggtatggcga caatgaagca atcaaaggag tggatctgga atttgaaaaa
    36421 aataagatca ctgcactgat tggtccttcc ggctgcggaa aatctacgta tcttcgttcg
    36481 ttgaatcgaa tgaatgatgg gatcgctaat acgaaaatca ctgggaaaat catgtatcaa
    36541 ggcgtagata ttaatgcgcc tcaagtggat gtttatgaga tgcgcaaacg gattggcatg
    36601 gtttttcaac gtcctaatcc gttcagcaag tcgatttatg acaatattac atttgctttg
    36661 aaacagcacg gcgaaaaaaa caaaaagaaa ttagatgaaa tcgttgaaac aagcttgaaa
    36721 caagctgcct tgtgggacca agtaaaagac agcttggaca aaagtgcctt ggctttatct
    36781 ggcgggcaac agcaacgttt atgtattgct cgtgcgatcg cgatgaaacc tgatatcttg
    36841 ctgctagacg agccggcaag tgctttggac cctatttcga caggaacggt tgaagaaaca
    36901 cttgtaaact tgaaagaaga ctatacgatc atcatcgtga cgcataacat gcaacaagca
    36961 gctcgtatca gcgactatac tgcctttttc tatctaggaa aagtaatgga atatgacaaa
    37021 actaacaaga tatttaccag accaaaaatt caggcaaccg aagattatgt gtccggtcat
    37081 tttggttaag acggaggcct caacatgaca aaagaaatta tttcatctaa agatcttcat
    37141 ctgtattatg gaaagaaaga agcactaaaa gggatcaatc taagtttcaa tcaaggggaa
    37201 atcactgcga tgatcggtcc ttctggttgc gggaaatcca cttatctgcg ttctttgaac
    37261 cggatgaatg acctgattcc gaatgtaacg atcaccggaa gtgttttata taaagacaaa
    37321 gacatctatg gtccaaaaac agatgttgtt gaactaagaa aagaaatcgg catggtattc
    37381 cagcagccaa acccatttcc tttttctatc tatgaaaatg tcatctatgg attgaaatta
    37441 aaaggggaaa aagataaaaa agtccttgat caagtagttg aagaaagcct gaaggcagcg
    37501 tctgtttggg atgatgtgaa agacaaattg cataaaagtg ctttatcttt atccggtggg
    37561 cagcagcagc gtgtatgtat cgcacgtgtt ttggcagtta atccggaaat catcttattg
    37621 gatgaaccaa caagtgcgct tgatcctgtt tctactggga aaatcgaaag tatgctgtta
    37681 gaattaagag agcggtatac gatgatcatc gtgacccata atatgtctca agcttcacgt
    37741 atttcagata agacggcatt ctttttagat ggtcacttga tcgaatacaa cgatacgaag
    37801 aagatattca tgaatccaga aaaacaagag acagaagatt atatttcagg tcgatttgga
    37861 taaggaggaa acggaatgct acgtacacaa tttgaagaag aattgctgaa tctccataat
    37921 cagttctacg aaatggggat gatggtgagc agcgccgtgc acaagtcagt tcgtgcatac
    37981 atcaaacacg ataaaatctt agcccaagaa gttatcgaaa acgatataaa tatcaataac
    38041 atggaaacac gcttggagaa gaaaagtttt gaaatgatcg cattgcaaca gccagttaca
    38101 actgatttgc gtatgatcat tacggtcatg aaagctagct cggatttaga acgtatggcc
    38161 gatcatgcgg tgtctgttgc taaatccacg attcgcgtaa aagggcagac gagaataccg
    38221 gaaatcgaaa aagagatctc cgatatgtcc gattatgtga aaaaaatggt ggacaacgtc
    38281 ctagtagctt atgtcaaaac agacgaaaaa gacgcacgta cgatcgccaa tatggatcaa
    38341 agagtcaatg actatttcaa tcgaatctat aatgcaacaa tcaaaaatat gcaggaaaat
    38401 cctgagacag tcatcagtgg aacagactac ttgaatgttg ccagttatct agaacggatc
    38461 ggagactatg tgacaaacat ctgtgaatgg atcgtctact tggctacagg gaaaatcact
    38521 gagttaaata cgaaccacaa tgagattttc taaactgaag ctgtgattat gccgtttgtt
    38581 taggaaaaga ggttgtgaaa tcagcggttg tgaaaaagct gttctgcatc aagtaataag
    38641 aacagtcaaa caaaaaatag ctcctcatat ttttcgtttg gctggactta ttaccgcaga
    38701 tgcaagcttt tgaacaccgt ttattcggga aagggtttgt gacaaaacat gtcacaaacc
    38761 cttttcatca tttcatagaa aggataaaga atgcaaaaaa aaaattagca tcatcactat
    38821 attttttatt tgtgtgctta cttcatgggt tattcagcat caagctgctt tatatgaagt
    38881 acctgtcatc gagatcgaaa aagtacgaat aaaagaggat catcaattga ttacaggaag
    38941 actgatgaac acggatcaaa aaggagaatc gatttattta tcggttcctt atcaaaaaaa
    39001 tcaaatagaa acagcaaata taaaagcgag acaaatgtat ctagtccaaa tgcatggaga
    39061 tgaatggcag ctagtagcaa aaaaaaatga tggctggctt ttcttcttca caagtatctt
    39121 cattggcgcc atgttgctta ttggcggaaa acagggtcta tttgctttag gaagtctcat
    39181 catcaatatc gttttacttt acttttgttg ctcatcttta ctaaaacaga agggatttct
    39241 ctattaaata tatctatcct gttcgtcgtt atagggatga ttattagcat attggcttta
    39301 aatggatgga atagaagcag tataatcaaa atttcagctg cagttacgag tgttttttta
    39361 gcatttttta tttgtctttt gacaatgaat cattggaaag accaaggact gcgctatgag
    39421 gaactgaatt atctgacacg accgtatcgt tctgtttttc tttctagcgt actgattgga
    39481 acagctggag gaacactcga taccgttatc acagtcattg cgacattaga agaattgact
    39541 aagcgacaac cagaaataac agcgaaacag ttatggcttt caggaaggca agtgggtcga
    39601 gatgtgattg gctcgatgac caatatcctt ttgttcgtct atatcagtgg gtcagttcca
    39661 tcgcttcttt tatatttagg aaatcaatgg tcgttcaaag aaacgataga acagcattta
    39721 tcattggaat ttttacgcgt cattgctggc agcttaggaa tagtattatc gatccctatc
    39781 tcggtcctct tttttctgac tgtaaggagg ctgaaacgat gaatgtactt attatacttg
    39841 ttgggatttt agcaatcagc gtgctactta ttggcggtac gcaaggtatg tatattcttt
    39901 taggcttatt catcaattta gggatctttt tcctgttgtt attcggttat catcagaagt
    39961 ggccgatact ggcattgtcg gtcataggat ttttattgat tgctgtggtt atcttatttt
    40021 ttatcaacgg ttataatcta aaaatgcgtg cagcttttgt gagtatactg atctttttat
    40081 tttgtttcct actattgctt cctatcactg attttttagc gatccaaggt tttacatcgg
    40141 tagaactaga agaattatct ggcttagata agactttagc gattgatttt agattactta
    40201 cgagatcgtt gcttctgatt agtcttagtg gtgctgtttt agatgctagt gtagcaattt
    40261 ctagtgggac attcgaagta tatcaggcaa atccgcaatt atctttttac caattgcgcc
    40321 attctagttt cgctattgct aaaaaaatat tggcgtcaac agtaatgaca cttctttttg
    40381 cattcatggg aagttcttta gcgttgatcc tctggtttat tgatttagac attccttttc
    40441 agcaagtgat caatgaaaaa tcatttgttc tagaatatac gatggctatt cttaccacat
    40501 tgtctgcact acttgttctt cctttaacct gcgtgactac agcatatctc tttacaaaaa
    40561 agaaagaaca actcaagatg gagtaaaagt tacaacttaa gtcctatgac atttttactg
    40621 aatcagtgca taatatggat gtaaggaaaa gcttgcaaac aattttgctt aaggaggcaa
    40681 tttccatgaa agaaagagaa cgtattttag atttagtgaa aaaaggaatc ctttcgactg
    40741 aagaaggctt agatttatta gaaagcatgg caactgaaaa agacgaaaag caaataaaaa
    40801 aagaagcaga caaggtaact gctagtcata aagaaaaaga tcaggcaagt caattgatcg
    40861 ataaacttga aaacggcgaa gaagaaattt ctgagccagt tgatccaaag gaaagagaaa
    40921 gacaagatca agaaaactta gaaaagatct tagatgaact agcaactgaa gcgaataaaa
    40981 cttcggcgcg tctagatgaa gtaaatgctc aattcgctga caataaggca gcacgcaatg
    41041 agaaacagga agcattgatg cagctgaata caaaagaaga attaggcgag ttgacagaag
    41101 aagaattagc acagcgtcag acgttagaag cagaaatcaa agaattagaa gctagtggcg
    41161 atacactgtt agaagaacaa ttgaaattag aagcagaatt aaaagatatc cgtaagaatc
    41221 aatggtctga gaaaaaagaa acgtttactg ataagtttga attgccagat gattggaaag
    41281 atcaagcaac agatacactg aatcaagtag gcgaaaaaat gtcagaagct ggatcacagt
    41341 taggcaaatt cttgaagaaa acattccaaa ctgtttcaga aacagtgaat gacaacatgg
    41401 aatggaaaga tgtcagctta cgtgtccctg ggattgcgac aacgaaattt gagcatgaat
    41461 tttactatga agctcctgca gcaagtatcc ttgatatcaa ggcagcaaac ggaaacgtga
    41521 cgttaaaaac gtgggacagc aacgatgtga aagttgaagc taagatcaaa ctttacggaa
    41581 aaatgggtgc tgaaccattt gaagcattct ctgaacggag ccagattgag gttaatgaag
    41641 atcatatctc cttccaaatt ccaaataaac gtgtgcgtgc agaccttgtt ttctacttac
    41701 ctaaacgagt atacgaccac gcagctatta aacttctaaa tggcaacatc atgattgaaa
    41761 cattggaagc taaagatatt tatacaaaat ctacgaatgg taacatcatc gtggaccaat
    41821 taactgcaac gatgctggaa gtagaaggtg taaacggcaa tatcgatatt cgaaacggga
    41881 acatcttaga ttctattatc gaaacagtaa atggtacagt gacatttggt gcgacagcag
    41941 aaaacctttc tgtgtctttg gtaaatggtg atgtacgtct tacgattaaa gaagataatt
    42001 tgaaaaaagt agaagcaagt tcagtgaacg gaaatgtcaa agtcgcttta ccagatggca
    42061 tcggtttgga aggacatgct aaaacaagtc ttggaagtat caacagccgt ctgtccaatt
    42121 acgaagtagt tcgtgagaaa aaagaacgga caaaccaaat gttgcaattc cgtcgtgttt
    42181 cagatggtga aatcacgcaa gtgcaacttt caacgacaac aggaagtatc tatttaaaag
    42241 atacagataa ataaagaggt tgtgaaagag atgttcagcc ttgagcaata agaagcgatt
    42301 ttgtaaaata gcttccccta ttttacaaaa ttgtgcctta ttgccgatag gctatctctt
    42361 gaacaccgtt taccaagaga ttatgaacct gccaattatc gccgagtcac aaggaataat
    42421 ttcagaaaaa gctcgtcacg ttttatgaat atggtaattt gtgaccgaga attttgcaga
    42481 aagacaccga ttaagaagaa ggttgtcaca acgattttct tcatttaaaa tagtagaaca
    42541 gaggtgagac atgatgaaaa aaaagttgac aaaatccaat aaaaatgttg ttttgacagg
    42601 gactttagca ggaataggag aatacatcgg gattgatccg actgttatcc gtgtgcttta
    42661 tgtattcctg agcttagttg cttttggaag tccaatcatt ttatacattt tattagcttt
    42721 gattatccca tctgcgcgtt ctgcacgaac aaactatgga cataacaatg catattatga
    42781 aaaaaacaat tatcgtgaac aaactcgtga acaaacaaaa aaacgcaaag aagcggaaaa
    42841 aatcgatgat gacgaatgga gtgactttta atgtcgtact ttcaacgtat tttagttaat
    42901 acgttaacat tcatatcatt ggctgttctt tttcctgcga tggtccatgt gagcagtatt
    42961 tggacggccg ttcttgccag cttcgtattg tctgtgttga atatgtttgt caaaccgatt
    43021 ttgacgatac tgtccctgcc ctttacatta gtcacattgg gattgtttac gtttgttatc
    43081 aatgcagcga ttcttaaaat gacctccttt tttgtgggag cagaacattt tagtttttct
    43141 tcattcggtg cagcgttgtt agtagctatc ttgatgtcgc ttgtcaatgt aattgtcagc
    43201 gaacataaca gtagaaaata aaagaaagct gtgccgaact atcggcgcag tttttttatt
    43261 tagaatcagg aatgcgtttt ctattacatt ggtaattaaa taaaaacgtt ttttttaatt
    43321 ggtagtactt ttggctagac aatgataaaa tgatagagaa cactctaata gaaatgaggt
    43381 agcatgaatg tcagaagttg taaaagttag ccaattagtg aaaaaattat ctttggaaat
    43441 cgtgacaggc gacgagaaaa gcttagaccg tgtcatcgta actggggaca tctcacgccc
    43501 aggactagag ttgactggtt attttaatta ttattcccat aatcgtttac agctatttgg
    43561 cagtaaagaa atcagttttg cggaacggat gatcccagaa gagcgtttga tcatcatgag
    43621 aagattatgt agtgaagata tgcccgcatt tattatttca agaggattac cggctccaga
    43681 agaaatgatc caggcagcaa atgaaaatgg gatagctgta ctgcgttctc ccatctcaac
    43741 ttctcgttta ttaggagaat tatccagtta tttagatggt cgccttgccc ctcgaacaag
    43801 tgtccacggt gttttagtgg atgtttacgg attaggtgta ctgattcaag gtgacagtgg
    43861 gataggaaaa agtgaaacag cgttggagtt gatcaaacgc ggtcatcgat tgatcgcaga
    43921 cgatcgagta gatgtttatc agcaagatga attaacggtg atcggagaac cgcctaaaat
    43981 cctggaacat ctaattgaaa tccgaggaat cgggatcatt gatgtaatga atctttttgg
    44041 tgcgagtgcg gtacgtggct ctatgcaagt ccagttggct gtttatctgg aagcatgggc
    44101 aaaagataag aaatacgacc gtctgggctc tgaagacacg accgttgaaa tcgcggaagt
    44161 gggcattcca caaataaaaa tccctgtcaa aacaggacga aacgttgcaa tcatcattga
    44221 agtagccgcg atgaatttcc gtgcacgtac aatgggcttt gacgcaacga aaacatttga
    44281 agaaagatta agtcgcttga ttgaagaaaa ctcaggcaac gactagcaag gagtgacaat
    44341 atgttagcac aaatcaatcg agttgctttt gagcttgtag ggattcctat ctactggtat
    44401 gcattgatca tcgtatcagg tatcattatc gccatgtggc ttagcagcag agaggctgtt
    44461 cgggtaggat taaaagcaga tgatgtgaca gattttatgt tgtggggatt gcctatctcc
    44521 attattggcg ctcgcttata ttatgtttta tttgatcttc cccagtatat agctgatcca
    44581 atccaaatat tcaatatccg ttcaggaggc ttagctattt atggcggact gattgcaggc
    44641 gcgatcacgc tgtatttttt tactaaatat cattttattt ctttgtggac ttttttagat
    44701 atcgctgcac caagtgtatt attagcacag gcaatcggtc gttggggaaa tttcatgaac
    44761 cacgaagcat atggaccaga cacgacgcga agcttcttag aaagtctgca tttaccgcaa
    44821 ttcatcattg ataatatgta tatcgaaggg atctatcgtc aaccaacatt tctttatgaa
    44881 tcactttgga gtttgattgg atttattttg cttttgtttt tacgaaagaa accacacctt
    44941 ttgaaaaaag gcgaagtcct tttatgttat ctaatgtggt atagtttcgg gcgtttcttt
    45001 attgaaggat tacgtacaga cagtctttac tttttaggtg tgatccgtat ttctcaatta
    45061 ttatctgctg tgttatttgt cggtacattg attctattta tttggcgcag aaaaaaagga
    45121 aatttaccgg attatgaccg ctcattagga aaaaatcaac aaattatctg atttttagag
    45181 cgttaaaaat gaaaaaaatt tcaggagtgg taaaatgaat tatagagtag cggttttagg
    45241 accaggctct tggggtactg ctttggcaca agtcctcgcc gaaaacggac atgacgttcg
    45301 gatttgggga catcgtgctt cacaagtaga agaaatcaac acccaacaca cgaatcaacg
    45361 ttatttacca gatcattatc tacccgattc catccaagca ttcaccaata tggaagatgc
    45421 agtaaaagac gcagatgcgg tgctattcgt cattccaacg aaagcaatac gttctgttgc
    45481 aaaagaacta gtgcctaaat tgaatacaaa gccagtgatc atccatgcaa gtaaaggatt
    45541 agaacaggga acacataaaa ggatctctga catcttgtta gaggaaatcc cagcagaaaa
    45601 aagaaaagct gttgttgttc tttctggacc aagccatgca gaagaagtag cgaaacatga
    45661 catcactacg atcacagctg caagtacaga tgcaaaagca gcaaaatatg tacaagagct
    45721 gttcatgaat aattattttc ggatctatac caatcctgat gtgatcggtg tagagatggg
    45781 ggcagctttg aaaaatatta ttgctattgg tgctggcgct attcacggct tagggtttgg
    45841 cgataatgca aaggcagcaa tcatgacgcg cggattagct gagatcagcc gtttaggtgt
    45901 agcaatgggt gccaacccgc tgacctttat cggattaagc ggagtaggcg atctagtcgt
    45961 tacttgcaca agtgttcatt ctaggaactg gcgcgcaggc aacttgttag gacaagggca
    46021 gcccctagaa gaggtgctag ccaatatggg aatggtcgtt gaaggtgtga atactaccca
    46081 agcagcgatg gaactagccc aaataatgga aatcgaaatg cccatcactc aaacaatcta
    46141 tgaagtgctg tatgaacaaa aagaaatcaa aacagccgca aaagaaatca tgttacgtga
    46201 tgggaaaaaa gaaaatgaat tctcattaac aattttttga aggagcgaat gtatatgaaa
    46261 gtcaaaaaag cagttattcc agcagcagga ttaggaacaa gatttttacc agcaacaaaa
    46321 gctatggcaa aggaaatgtt gccaattgtt gataaaccaa caatccagtt catcgttgaa
    46381 gaagcgttga aatcaggaat cgaagacatc ttgatcgtta caggaaaagc taaacgacca
    46441 atcgaagatc actttgattc aaatattgaa ttagaaacaa acctaagtga aaaaggaaag
    46501 accgaattat tgaaattagt agaagaaacg acagatgtca acttgcattt catccgtcaa
    46561 tctcatccaa agggtttagg acatgccgtt cttcaagcaa gagcctttat tggaaatgaa
    46621 ccgtttgtcg ttatgctagg cgatgatatc atggaagatg aagtaccatt gacacagcaa
    46681 ttaatcgatg attacgaacg tactcatgct tctacgatcg ccgttatgca agttcctcat
    46741 gatgaaactt caaaatacgg aattatcaat ccaggagaag tattggaaga tgggttatac
    46801 aatgtcaaaa actttgttga aaaaccagat ccaagcaaag caccaagtga tttagctatt
    46861 attggacgtt acttattgac tccagaaatt tttgatgtat tgtccgagca acaaccagga
    46921 gccggaaatg aaatccaact aactgacgca atcgatacat tgaataaaac acaacgcgta
    46981 tttgcccgta aattcacagg taaacgttac gatgtcggtg acaaatttgg ttttatgaaa
    47041 acaagtattg aatatggtct aacacatcca gaagtaggtg cacctttacg tgagtatatc
    47101 ctgtctttag gtaaaacatt agaagcagaa ggaagcaaga tcgatcataa tccaaaagga
    47161 ataacagaaa tcaaagaaga aaagaaaaaa taaccctttt ttctaacgag cttcagctgc
    47221 taattgaata aaaagtagtt gaagctcgtt tttatttgtt taaaaaacac gaaaatgcta
    47281 tccttataga aaagagagac aagaagggag tttgatcatg tcaggaggag aaattgcagg
    47341 attgattgca gcaatcgcct ttgcgatact tgtcgttttt atcgttcgtg tgttgttgca
    47401 agtttctaaa actttagaaa aagtagatca gactgtagca gaagcaaata cgacgatcga
    47461 aatcgtcaca aaagacgtag atcttttgtc tagacaagta gaaggcttgc tggtcaaaag
    47521 taatgaactg ctcttggata tcaatggaaa agtcgcaacg atcgatccat tgttcacagc
    47581 agttgctgat ttaagtgaaa gtgtatcaga acttaatcat tctagtaaaa acatcgcgac
    47641 gaaagtcggc ggaatcggta aagcgacagc taaagcgacg gttgccggaa aagtcggtgg
    47701 gacagcgatg aaatttttta aaaacaaaaa acatactgaa acgacaggag gaaaataaaa
    47761 atggctaaaa aaggtggatt tttattaggt gcagtagtcg gaggaacagc cgcagcaatc
    47821 gccgcgttat tgttagcacc aaaatctggt aaagaacttc gggatgaatt agctgcccaa
    47881 gcggatgatt tgatggatat ggcttcagat tacacagata tggcgaaaga aaaatcgact
    47941 gagttaacag gtattgcaaa agaaaaagcg caagatcttt ctcaacaagc aggtgatcta
    48001 gcaggtagtg tcaaacaaaa agtaagtgaa tcagcagatg aagcaggaag tgaatctgac
    48061 gatatcttgt taaatcttaa agaacaatca gctgacttat ctaatcgttt caaaaaaacg
    48121 gcagaagata tgaaaaaacc tgcagatgaa ttaggaagta ttgctaaaga tacttcagaa
    48181 gatatcatga ttgatgtaaa agacgctgct ggtaaagcag gagatactgt caatgaagga
    48241 atgtcagaag caaaaccagt tgcagatgac acaaaagaaa tgatgaaaat ggcaaaagaa
    48301 gatgcgaaag aagctaaaaa agaaataaaa gatacaatga cagattcatc tacaaatcac
    48361 taaaatcaca aatttataga ataaaaaggt gggagcggac tagctctcac cttttttact
    48421 cttatttttc taaaacaagt aattcttttg gtgtagtcgt gaatggcaca cagccatcgc
    48481 ttgtcacata tacacagtct tcgatacgta cccctacttt ttcaggaata tagattcctg
    48541 gttcaagaga gaagcacatc ccttcttcga ttactaagtc atttccttct acaagggaag
    48601 ggaattcgtg gacggttgtt ccgatcccgt gacctaaacg gtgattgaaa tattcgccgt
    48661 agccatagct gttgatcacg tttcgtgcga tttgatcaag ttcacctgct gtcacacctg
    48721 gacggacggc ttcttgtgcg gcaagctgtg cttcaagagt gatgttgtat attttttctt
    48781 ggaagtcggt tggtttttga taagcaattg tacgagtagc atcactgcaa tagccattcc
    48841 aaacaacgcc aagatcgaat aacacaagtt cattcggtga aatcttcgtg tttcctggtg
    48901 taccatgagg gcttgcagca tttgctcctg caaggactaa tgtgtcgaaa gacatttgtg
    48961 aaacaccttg ttttttcaat tggtattcga tttctgcaat gatcgcttgt tcagtcacgc
    49021 cttctttcac tgctttaaag ccaatctcaa atgcaacatc ggcccattta ccagcttcga
    49081 tcagtttgtc tttttcctct agagttttga tcagttgtag acgttggatg atggaagtta
    49141 gatcagtgta agaatccgtt cccgtcaaga tatcagagat agcatcgaag cgctcgacag
    49201 tcaaggcgtt cttttcaatc cctacattgc caggctgacc atagcgagca cgaagttctt
    49261 ttccgatgat ctcccaaggg ttttcgctgt ctaaatagcc acggacatca tacgcccaac
    49321 tactgttttg agcatcttca acttccaaag caggagtgaa aaggaaagga tcttgttcaa
    49381 gagggataaa aagagctagt acacgctcat gaggatcact ttcgtatccg gaaaagtaag
    49441 cgatgtggcc agggtcgctg atataagcca attcgacttg gtgttgtgcc atccattgac
    49501 gtagttcatt gatttttacg ttattcattt ggcaacctac tttctaaatt aattttgtat
    49561 tcaataattg taccactgat ttattaaaag accaacgttc taaaaactga aaaaagtaaa
    49621 aattttcatg aaaacagaga taaaacggtt gcaattcttg agattatttg atattctatt
    49681 ttagaaatgt aagacgtatt ttcagaaaac atataaaata tgaataggag aacacaaatg
    49741 gaaaagcaaa caatcactat ctatgatgta gcgagagagg caaatgtttc gatggctact
    49801 gtttcacgtg tcgtaaatgg caatccaaac gtcaaaccag caacaagaaa aaaagtcttg
    49861 gaagttatcg atcgcttgga ttatcgtcca aacgcagttg cgcgtggctt ggcaagtaag
    49921 aaaacaacaa cagttggtgt aatcatccct gatgtcagca atatgttctt tgcttcttta
    49981 gcacgtggta tcgatgatgt ggcaacaatg tacaaataca atattatctt agcaaattca
    50041 gatggcaacg accaaaaaga agtcaatgta ttgaataact tactggctaa gcaagtggac
    50101 ggcgtgatct tcatgggtca ccatatcaca gacgaaatcc gtggagaatt ttcacgttca
    50161 aaaactcctg ttgtattagc tggctctatt gatccagatg aacaagtagg tagcgtaaac
    50221 attgattatg tatccgcaac aaaagatgca gtaaacaaat tggcaaaaag cggcaataaa
    50281 aaaatcgctt ttgtcagtgg tgcgttgatc gatcccatca atggacaaaa tcgtctgaaa
    50341 ggctataaag aagcattaag cgaaaataac ctaccatata gcgaaggatt gatctttgaa
    50401 gcacaataca acttcaaaga tggtctatct ctagtcgatc ggatccataa tagcggtgca
    50461 acagctgtct atgtttcaga tgatgaatta gccatcggta ttttggatgg tttattagac
    50521 cgtggcgtga aagtaccgga agaattcgaa atcatcacaa gtaacaattc actactgaca
    50581 gaagtagctc gtcctcgttt aacaagtatc acacaaccat tatacgattt aggggcggta
    50641 tctatgcgcc tattgacgaa aatgatgaat aaagaagaag tcgaagaaaa aacgattatc
    50701 ttgccttatg gaatcgaaga aaaaggatct acaaaataat aaaaggacaa ccattcagag
    50761 gttgtgtgcc tgccgtttaa ctctgcgtta caaggagcaa tttcaaaaaa cagcatctca
    50821 tgtttgatga atattgtgac tcgtgattcg gagttggcag gagaacaccg tttatttggg
    50881 aaaaggggtt atgacaaaat tttgttataa tccttttttt gtgtcttaaa aaagagagaa
    50941 agtggttgaa attatattta tcgtatataa aacaatccgt gtatagatgc tgtattttgt
    51001 tcaccatcat caaaaaaata agggaatgtg acaatactat tgtcacattc ccttattact
    51061 gtgtattcta ttaaatcaat catctttctc atctgattct ttggttgctt gattcgctct
    51121 tgcataagct gaagcggtgt tccagtaatc tgtatagttt tcatcggtac cgccaacgcc
    51181 aaacttaaag gcgcttttct tcggtccatt tttcgcccat agcgattcaa ctgtttttcc
    51241 tggtgtttgg atggattttt tattgacagt aacttttcca ggtgtttgac cagtaaatcc
    51301 agatactttt ttcttcatca catcagatga taattcgaat ttctcatcag tgccaaaaat
    51361 gtcaggtttt gcttggtaag cttgattgat cagattggca agataattag ccgaacgtat
    51421 ccttgctttg ctgtccattc cagtattgtc atcgtgccct gcccagctac tgatggtaat
    51481 agacggagta gagacaatta gccatgaatc tcggtattca ttggtagaac cagtttttcc
    51541 gacccaatct gcttttccta gatttccgtt taagcttgag atggcatctt tgaatggtgt
    51601 agtgattttt gcattgatca cactgcgcat catgtcattc atgatcgatg ccgttgcttc
    51661 cgaatagatc cggacagagt tgctttcgtg tttataaatt acatttcctg cgttatctgt
    51721 gatggaatcg atgatatagc cttcttcata gacgccgccg tttgctaaag cctgaaatcc
    51781 gttggtctgt tgaagcgtcg tgacatctac tgtacctaaa ggagcagatt caacgcccca
    51841 attgtcattt gccgggtagt tcatctttga caagtagtgc tcgtaagcat attgtttcga
    51901 tccaccggaa ttcaacacat cttgatataa gtgataagcc gggatattgt tggaccattc
    51961 tagtgactca cggacggttt ggaaggtgtt cgatccttcg ttagtagcat tgacgatttt
    52021 ctcgccagca ttttctcctt cttgccaagt tgttgcataa tctgatacac gggattctga
    52081 tccgataagc cccatgtcga ttgcaggtcc gtaaacaaga actggtttga ttgatgagcc
    52141 agcttgacgt tctgtatcga aggcatggtt attttgattc tgactgtaat tccgtccgcc
    52201 aacgaatccg tagatgcggc cagttctgtt atccatcaat acatttccgg tttctacttg
    52261 actgccagca ccatcatcta acatgtatcc ataattttca acgccatttt gcaatgcagc
    52321 atagacatct ttatcgattg tcgaatggat tgtatatcct ttattttgga tcgtttgctg
    52381 tgccttttga taataagcgt cactgatcga tgaatcagac atatcagcgt tgtctttttc
    52441 agccaattgt tgtgcaatga ttcttgttgc ttcattcatc acagtataat acaagaattc
    52501 tctgtcagtc tgttctgcga tttgttgtgg tagaaagtct tttgtaaggt cataggcttt
    52561 tgcttcttcg tattgttctt tcgtaatttg gccttcacga tacatactga ataaaacaaa
    52621 atcttttctg gcaaggccgg cagaaagatc ttcttttagc gcacctgtgt tcgtgtatgg
    52681 actatacgtg atcgggcttt ggggtaatcc agcgatataa gcagcttgcg gcagagtaac
    52741 atcttttgca tctacaccaa agattcctct tgcagcttcc tggactccag caatgttttg
    52801 tcccttatta ttacgaccaa atggtgagac attcaaatac gtagcaatga tctcatcttt
    52861 tgaaaagtat ttttctactt gtgcagaaag aatgatctcg tttgctttac gtttgaatgt
    52921 tgtttcatct gtaaggatct gctgtttgac taattgctgt gtcaaagttg atccaccaga
    52981 agagccaata ccagtagctt cagatactaa tgctcgtaaa acagctttag gaacgtaacc
    53041 ttggtgttta tcgaagtatt catcctctgt gctgataatt gcagttttca ataaaggtga
    53101 aattttatca gatgaaatag tcgtgcgcat cagatctgtc tggatttttg atatctctgt
    53161 attatctgca taaacaatct ttgacgtttc tgtaatgttt cctagttctg tctgcagtgc
    53221 ttttttagtc ggcaattgtg tgtcttcgac gagataggca aaatagccag cgcctattcc
    53281 aatacctaga gctgcgaaca tcaaaagaag aacagtaata aaaacagtca atgattgaaa
    53341 aactctaaaa ataatcttag gaacaaacca tttttctttt tttcttctat tctgtttttt
    53401 tcgagtattt ttgcgtggca aaagggtcac ctcaactaat ggttttattt cgctaattat
    53461 agcaaataat agaacaaaaa aacagacaag gctgtttttg gcaggaattt cttttttaaa
    53521 ggagttaaaa cgtgagaaaa tgatcttttt tataaaagag ttcttaagct tttctttaga
    53581 gaaaatttca gttactatgg agaacagggc aaagaaaaat caatcatttt ctttgtatat
    53641 catcttacca atatatagga attgttagta acaattgcct ttcaaggagg aatccatcca
    53701 tgttattagg ctcacacgtc agcatgagcg gaaagaaaat gctgttagga gcagcagaag
    53761 aagcagccag ttatggatct tcgactttta tgatctatac aggcgcccct caaaacacta
    53821 gaagaaaagc catcgaagag atgaatattg atgccggaaa agcgtacatg gcagaaaacg
    53881 gtctaacaaa tatcgtcgtt catgcccctt atatcattaa tttggggaat acgatcaaac
    53941 cagaaatgtt tccttttgcc gtccaatttc tgagagaaga gattgttcgt gcggaagctt
    54001 taggcgctac tcaaattaca cttcacccag gtgctcatgt aggggcagga acagcagccg
    54061 gaattgctag aattattgaa ggcttgaatg aagttttgac aaaagaacag acggcacaaa
    54121 tcgctttgga gacgatggca ggtaaaggta cggaaatcgg tcgaacattt gaggaattag
    54181 cgcaaatcat cgatggagta aaattgaatg aaaaattatc cgttactttt gacacctgtc
    54241 atacgaatga tgccggttat aatattaaag aagacttttc aggtgtaatg gaagagtttg
    54301 ataagatcat cgggttagat cggcttaaag tactccatat caatgattct aaaaatccgc
    54361 aaggttcgca taaagaccgg cacgccaata ttggttttgg aacaatcgga ttcgacacgt
    54421 tgaataagat cgtccatgat ccaatgtttg acaatatttc gaaaatcttg gaaactccct
    54481 atgtcggcaa agataaaaag aaagcttacg caccatatgg aaaagaaatc gccatgttca
    54541 aagcgcaaac attcgatcca gaagtgtttc ctgagttatt agcagaagaa gtgatactct
    54601 aagattttct atttgacagc agcaaaaaag gaccaggcac attgccttgt ccttttttgt
    54661 ttaagaaaca aaggagtact ttttgcagtc tttttcatca tgcatcttct cccccctctt
    54721 tttaaattta gacaaacagg gtatactggt agaaaagtaa aaaaacgaaa agtttcacaa
    54781 gcaagtggca aaacgtgatt ggaaggacga aaagatgcta aacactttaa aacccttgat
    54841 caaccaactg actaagaaac atacatactt tattcttggc atagccctat tatttacaat
    54901 tattcgtttg tatttatata gtaaagcaat ctatggaaca gacatgtcgg caacctatga
    54961 tgatcagttg ctgattcgct atgctcaaaa tattttggac ggtaaatggc taggggagta
    55021 tacgactaca acactttcca aaggaatctc ctacagctta tttttagtac ttgcacataa
    55081 actgcatttt tcttatgcag ttttattcgg catgttcagt atttttgcca gtatattggt
    55141 tgcactcgcg tttaaaccga tcgtgaagaa caagtatctt cttttaagta tctattttta
    55201 ttttttgttt tctccgatta gttttacaca tgaatattcc actcgaacgt atcgtaatgc
    55261 cctcgtgatt ccggctgttg agatcattat cgcttgttta ttagctattt attaccgcaa
    55321 atacggttct agcagaaaat tgctgccatg gtttttaggg ctttctgtcg cttttccttt
    55381 tttttggttc attcgcgaag attctatctg gcttcttcct tttactgtag tagctttggg
    55441 tatcagtttc gtccagatct tgctagggca agacttttct ttcaaagaag taaagaagtt
    55501 aaaaaaacga ctctttcttc tctcgaaacg ggaatggctg aaactttcat tgtttttact
    55561 tcctatagtt ggcttttcca tcgtctatca gtggatcgaa tataaaaatg agacaacata
    55621 cggtattgca accatcaatg accgttcgag tggagaattt ggacgtttga tgaaacaact
    55681 gatccgaata gatgatgaaa cgaacttgaa taaagaaaac agtgagatat gggtctcaca
    55741 tgctgcacta aataaagcat tagccgcatc tcctacattc catactgttt ctacccgtat
    55801 tgacgaatta tatacgactc acgcgtggac tcaaggtgga aaagtaaaag aattacccgg
    55861 agatatcatc ttttgggcat tgcgagatgt agtaagcgaa tctggttatt accaaaacaa
    55921 tgcaaaagaa accaatgatt tttggaaaaa agtcaaccaa gaattagaag aagcctatca
    55981 caagggaacc atcaaaaaga aaaaagagtt ctatctgatg ggatcaggag atggaaagac
    56041 gatgagtgat ctgccaatcg tattcgattt ccttcaatca ggatacgctt atgcatggcg
    56101 atataaagat ttccatcaag gaggaaatta tagtttcgga acgcaagaac aagtcaaaca
    56161 ggctcaagaa ttattaaacc ttccgctaat ggagaattgg atcgatagta atgaaccgag
    56221 aacacctaac ttccaattaa caaaatcagc aaaagtcgcg aatgtcatca taaaaattta
    56281 ccaagttctg gctattcccg tgctgattgc ttcaatagca gggatattac ttatgatact
    56341 aggaaccgta tttgcaaaag ctgatcgtgc ctattatagt tcctctttga ttgtggtcac
    56401 cggattaata ctgacacaat tggtttttct tttcggtgtc tcatggtttt gttcttatgc
    56461 gccagaaaaa aaggactatt ttttaagtgt ctacactggt gcaggcgtgc cattaacgca
    56521 attcatcgta gtaacaagca tgctagtttt cagtaaatta aatctgaaaa aagctttatc
    56581 ttctaaataa aaagaccctt ggtagctaga tttttaacct aactatcaag ggtctttgtg
    56641 tggaaaaatc agtttttatt cgatttcttc taccgtttcc tgcaaaagct gttcatattt
    56701 tttgtttcct ttatataaat caccaatcgt ccatgccaac aggagaagga caagaacaat
    56761 gattacataa gcgatgtgat cagccaatgc cagttgagcg gaagtggctt gctcgccgaa
    56821 gaaactttcg atctgtcctg gtaatcctcg taagttcaag taagttagac taatcacaga
    56881 gaaccagcca agaagtttta cgatgaatga atttttaaat cggctaccca tctccgcttg
    56941 gctgttggtc atcatcagta aaggaatcat cgaaaatggc aacgcaaaag ccaagaacac
    57001 ctgggattca ttcattaagt tattcaaagc ggtatgttct tcgatcgttc cttttgtact
    57061 tgtaaacaaa acacaaatca atacaggaat cacagaaagc aatcgtgtga ccagtcttct
    57121 tagccaaatc gggatacgca tatggatgaa tccttccatg atgacttgac cggtcaacgt
    57181 accagtaata gtagagttct ggccagaagc aagaagtgct acagcaaata aagtggacaa
    57241 gataccagat tttgctacag caatcaaaat accattgctc atagctgatg gatcagataa
    57301 agcttcatac agaccaaaaa aggaaggatc ttggacagaa ccggttttga agacagcgac
    57361 acccatgatc aatagcagtg cattgactag aaaagccatt gacaactgga tattagaatc
    57421 ccaagtagaa aaacgtacag cttgtgctac atcttcttcg ttttggtgat cgatcttccg
    57481 agtttgtgaa actgctgaat gaagatataa gttatgcggc atgactgttg caccaataat
    57541 ccctaaagct ccagttagcg gagtttcgcc accaatgaca ggtgagtctg agaatgtatg
    57601 gctgttaggg ataaagccag caaataaact gtgccaatct gggttagaca agccaacttg
    57661 atagacaaaa acgaataaaa tgacgaaaat taagcagaca accagtgctt cgatcttacg
    57721 aaacccgact tttgtcagta gtaagagaac gaaaacatct aatacagtaa taaagacggc
    57781 aatcaccaat ggaatatcaa aaagtaagta gagggcgatt gctgcaccaa ttacttctgc
    57841 aatatctgtt gccataatgg ctaattcagt caagatccat aaaatgacac ctaatgtctt
    57901 acttgtgcgt gctcggattg cctgtgccaa atccatttgg ctcacgattc ctaattttgc
    57961 tgccatatac tgtaaaagca tagcaataag actagacatc aaaataactg acatcaataa
    58021 atattggaag ttttgtcctc cggtgatcga agtagaccag ttacccggat ccatataccc
    58081 aactgctact aacgcaccag gtccagagta agcaagaagt gttttaaaaa aacttttccc
    58141 ctttggtaca tcaactgttc catttacttc agctaaagat aaaccatttg tatgttcaat
    58201 aagtcgatgc ttatgaaatt gtttattgga ttgattattc aagaaaaaac cacctttcct
    58261 atttatctac gattacattc tacacctcaa aaagaaaaaa ttaaagaaaa agattcggta
    58321 tgcctaattt attttcgtgg cgtgtcttgc tagatgacga aagaaatttt gttctcctct
    58381 aaaaaaaccg ctattttttg catgaaacta aagaatacgc ctttcttttt tcaataaact
    58441 ttagtaaaat aagtcattga gagcaattcg gaaatagaaa ggagagattt atatgttatc
    58501 aacaggtatt gtcgtactta ttgctattat tgctttgcta atcggcgcag taggtggctt
    58561 tttcctagcg cgtaaatata tgaaagacta tttagaaaaa aatcctcccg ttaatgagga
    58621 catgttgcga tcaatgatga tgtcaatggg gcaaaaacca tctgagaaga aaattcgcca
    58681 gatgatgcag caaatgaaga accaagggaa aaaataggtt ctgacccaac gactttttac
    58741 gggctgattt tttcagcccg ttttttattt cgtaatttgt tagactattc agattattca
    58801 gcaaaaaaga caagcacaga caatcctagg ctcttgataa agacaaagga ggagtaaaat
    58861 gtcgattttt agaaaattag gctggttttt tcgtcaggaa aagaaacatt atttaattgg
    58921 agttttctcg ctattcgcag tggccttggt acagttagtt cctccaaaag tgattgggat
    58981 catcattgat gagattgccg ataaaaattt atcaataaag atcattttcg ggtggatctt
    59041 cctattgatt atcgctgctt ttttacaata tctttttcgt tatatctggc ggacgaatat
    59101 ttggggaagt gcagctcggt tggagaaaga acttcggcag cagttattca aacattttac
    59161 agaaatggac aatctctttt accaaaaata tcggactggg gatttgatgg ctcatgcaac
    59221 gaatgatttg aatgcgatcc agaatgtagc aggagctggt atcttgacct ttgcagattc
    59281 agtgattacg ggcggaacaa cgatcattgc gatgattttg tttattgatt ggcgcttaac
    59341 actgattgcg atgattcctc tgcctttgct ggccatcacc tcacgagtgc tgggatcgaa
    59401 actccatgat gcttttcgtg attcgcaaga agctttttct agtatcaatg ataagacaca
    59461 agaaagtatc actgggatca aagtcattaa aacatttgga caagaaaaag aagatctagc
    59521 ggattttgaa gaaaagattg aagatgcaat tattaaaaac aaacgagtca attttttaga
    59581 ctccttattt gatccattca ttaccttgat tatcggtctg tcttatgttg caacaattat
    59641 tttaggtgga aagtatgtct tggaaggcac gatctcgatc gggcagatgg tctcatttat
    59701 tagttatatc ggtatgcttg tttggccgat gtttgcgatt ggtcgattgt tcaacgtatt
    59761 ggaaagagga aatgcttctt atgatcgcgt caatgaactt ttgcatgaaa aaacgcatat
    59821 cgtggaacaa ctgggtgcag tgcagacccc ggcaaaaggc gagctgaatg tagctatttc
    59881 ctcttttaat tatctgaaag aagaatcacc agcattagaa caaatcaagt ttgcaatcca
    59941 agaaggagaa acattaggta ttgttgggaa aactggtgcg ggaaaaacga cgatcatcaa
    60001 attactgatg cgtgaatacg atgattatca agggaaaatc caatttggcg gacgttcgat
    60061 caaagaatat tcattgaatg ctttattagg atcaatcggc tatgtgcctc aggatcactt
    60121 cttgttttcg atgaccattc gagataatat ccgtttttct aatccaaaac tttcagaaga
    60181 agaagtaaag catgcggctt ctttagcatt tatcaacaat gaaatcaagg aattaccaaa
    60241 aggttatgat accttagttg gagaacgtgg agtctcttta tctggcggac agaaacaaag
    60301 gatttcgatt gctcgagcat tgatcaaaaa tcctgaatta ttaatgttag atgatgcact
    60361 ttctgcagta gatgccaaaa cagaagaagc tatcttgcat aatttaaaag aaataagaaa
    60421 agagaaaaca actatcattg cagctcacc
//
LOCUS       DACDEG010000009        58883 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.009, whole genome shotgun sequence.
ACCESSION   DACDEG010000009 DACDEG010000000
VERSION     DACDEG010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.009"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..196)
                     /locus_tag="IV142_000616"
     rRNA            complement(<1..196)
                     /locus_tag="IV142_000616"
                     /product="16S ribosomal RNA"
     gene            complement(719..2029)
                     /locus_tag="IV142_000617"
     CDS             complement(719..2029)
                     /locus_tag="IV142_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP8484296.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(2041..3459)
                     /locus_tag="IV142_000618"
     CDS             complement(2041..3459)
                     /locus_tag="IV142_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8484297.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3699..5267)
                     /locus_tag="IV142_000619"
     CDS             complement(3699..5267)
                     /locus_tag="IV142_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP8484298.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5271..5681)
                     /locus_tag="IV142_000620"
     CDS             complement(5271..5681)
                     /locus_tag="IV142_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP8484299.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5678..6601)
                     /locus_tag="IV142_000621"
     CDS             complement(5678..6601)
                     /locus_tag="IV142_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP8484300.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6598..7476)
                     /locus_tag="IV142_000622"
     CDS             complement(6598..7476)
                     /locus_tag="IV142_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP8484301.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7463..8800)
                     /locus_tag="IV142_000623"
     CDS             complement(7463..8800)
                     /locus_tag="IV142_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP8484302.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8803..9906)
                     /locus_tag="IV142_000624"
     CDS             complement(8803..9906)
                     /locus_tag="IV142_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP8484303.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9980..11488)
                     /locus_tag="IV142_000625"
     CDS             complement(9980..11488)
                     /locus_tag="IV142_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8484304.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11454..12146)
                     /locus_tag="IV142_000626"
     CDS             complement(11454..12146)
                     /locus_tag="IV142_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8484305.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(12168..13142)
                     /locus_tag="IV142_000627"
     CDS             complement(12168..13142)
                     /locus_tag="IV142_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8484306.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13442..14314
                     /locus_tag="IV142_000628"
     CDS             13442..14314
                     /locus_tag="IV142_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8484307.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14772..16970)
                     /locus_tag="IV142_000629"
     CDS             complement(14772..16970)
                     /locus_tag="IV142_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8484308.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            17202..18194
                     /locus_tag="IV142_000630"
     CDS             17202..18194
                     /locus_tag="IV142_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8484309.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            18254..19675
                     /locus_tag="IV142_000631"
     CDS             18254..19675
                     /locus_tag="IV142_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP8484310.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19668..21167
                     /locus_tag="IV142_000632"
     CDS             19668..21167
                     /locus_tag="IV142_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP8484311.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            21206..22600
                     /gene="uxaC"
                     /locus_tag="IV142_000633"
     CDS             21206..22600
                     /gene="uxaC"
                     /locus_tag="IV142_000633"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP8484312.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22759..23442
                     /locus_tag="IV142_000634"
     CDS             22759..23442
                     /locus_tag="IV142_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP8484313.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23444..24280
                     /locus_tag="IV142_000635"
     CDS             23444..24280
                     /locus_tag="IV142_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8484314.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            24291..26045
                     /locus_tag="IV142_000636"
     CDS             24291..26045
                     /locus_tag="IV142_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8484315.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(26321..26662)
                     /locus_tag="IV142_000637"
     CDS             complement(26321..26662)
                     /locus_tag="IV142_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP8484316.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26730..28901)
                     /locus_tag="IV142_000638"
     CDS             complement(26730..28901)
                     /locus_tag="IV142_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP8484317.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            29112..29969
                     /locus_tag="IV142_000639"
     CDS             29112..29969
                     /locus_tag="IV142_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8484318.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(30040..30897)
                     /locus_tag="IV142_000640"
     CDS             complement(30040..30897)
                     /locus_tag="IV142_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8484319.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30882..33584)
                     /locus_tag="IV142_000641"
     CDS             complement(30882..33584)
                     /locus_tag="IV142_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8484320.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33597..34886)
                     /locus_tag="IV142_000642"
     CDS             complement(33597..34886)
                     /locus_tag="IV142_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP8484321.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            35036..36082
                     /locus_tag="IV142_000643"
     CDS             35036..36082
                     /locus_tag="IV142_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8484322.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            36084..38237
                     /locus_tag="IV142_000644"
     CDS             36084..38237
                     /locus_tag="IV142_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8484323.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(38710..40161)
                     /locus_tag="IV142_000645"
     CDS             complement(38710..40161)
                     /locus_tag="IV142_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8484324.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(40158..41882)
                     /locus_tag="IV142_000646"
     CDS             complement(40158..41882)
                     /locus_tag="IV142_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8484325.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(41875..42489)
                     /locus_tag="IV142_000647"
     CDS             complement(41875..42489)
                     /locus_tag="IV142_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8484326.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(42834..44291)
                     /locus_tag="IV142_000648"
     CDS             complement(42834..44291)
                     /locus_tag="IV142_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8484327.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(44332..45252)
                     /locus_tag="IV142_000649"
     CDS             complement(44332..45252)
                     /locus_tag="IV142_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8484328.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(45264..46211)
                     /locus_tag="IV142_000650"
     CDS             complement(45264..46211)
                     /locus_tag="IV142_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8484329.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            complement(46690..47409)
                     /locus_tag="IV142_000651"
     CDS             complement(46690..47409)
                     /locus_tag="IV142_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP8484330.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(47458..49104)
                     /locus_tag="IV142_000652"
     CDS             complement(47458..49104)
                     /locus_tag="IV142_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8484331.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            49283..49873
                     /locus_tag="IV142_000653"
     CDS             49283..49873
                     /locus_tag="IV142_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8484332.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            49965..51470
                     /locus_tag="IV142_000654"
     CDS             49965..51470
                     /locus_tag="IV142_000654"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP8484333.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(51554..52906)
                     /gene="celB"
                     /locus_tag="IV142_000655"
     CDS             complement(51554..52906)
                     /gene="celB"
                     /locus_tag="IV142_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP8484334.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(52906..53424)
                     /locus_tag="IV142_000656"
     CDS             complement(52906..53424)
                     /locus_tag="IV142_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484335.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(53440..53766)
                     /locus_tag="IV142_000657"
     CDS             complement(53440..53766)
                     /locus_tag="IV142_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP8484336.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(53779..55758)
                     /locus_tag="IV142_000658"
     CDS             complement(53779..55758)
                     /locus_tag="IV142_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8484337.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(55779..56093)
                     /locus_tag="IV142_000659"
     CDS             complement(55779..56093)
                     /locus_tag="IV142_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP8484338.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            56261..56554
                     /locus_tag="IV142_000660"
     CDS             56261..56554
                     /locus_tag="IV142_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP8484339.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(56632..57744)
                     /locus_tag="IV142_000661"
     CDS             complement(56632..57744)
                     /locus_tag="IV142_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8484340.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            58131..58310
                     /locus_tag="IV142_000662"
     CDS             58131..58310
                     /locus_tag="IV142_000662"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484341.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            58504..58674
                     /locus_tag="IV142_000663"
     CDS             58504..58674
                     /locus_tag="IV142_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8484342.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
BASE COUNT        17659 a        12002 c        10313 g        18909 t
ORIGIN      
        1 gattgttata cggtattagc acctgtttcc aagtgttatc cccttctgat gggcaggtta
       61 cccacgtgtt actcacccgt tcgccactcc tctttttccg gtggagcaag ctccggtgga
      121 aaaagaagcg ttcgacttgc atgtattagg cacgccgcca gcgttcgtcc tgagccagga
      181 tcaaactctc ataaaaagtt cgaacaatct tgcgattgtt aagctcaatt tgtttgctag
      241 catattgctt gcttgttaaa aatgtttatt gtcttgaatt gaatcaagac gccctacaca
      301 tttggtttgt cttactttgt tcagttttca aaggtctact tcatcgagag tgtttcccga
      361 ttcatttttg gctgccgtag caacttttat atcatatcag agagacaaac gattgtcaac
      421 tcttttttaa aagttttttt cgatttcgtt tttcgaaatc tttcagcaac tcatttatca
      481 taacttatcg tttgttattt gtcaagaact tttttgaagt ttttttcgat agaatcaagc
      541 ttcgcaagtg gttcttttta acgacttagt tatcttatca cgtctaaaaa catgtgtcaa
      601 ctcttttttg gaattttcat aaaatgtttc aatgaagcgc gacaacgttg tttaatatat
      661 cattaaaaaa aataaaggtc aagctaaaat attatttaag cttgacctta ttataatttc
      721 aacgattgtc aaaagtattg tttgcagctg tgttgccagt ttctatagat aaatcacagc
      781 ttttgaaaca taagttttgt actgcttcga ttcgcccgaa ttcttttgca gttatttggc
      841 aatgagaaaa gcagacatct tctataggct tttcaggaaa tgctactaaa tcaaaagctc
      901 gggaaggaag tgagtaacct ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt
      961 tgactttcgt catttgcttt gattcaggta tttcttcagc cactgcttgc caactttctg
     1021 gcatttgagc aaggtcattg ctattttttc tattgtattc attatgccag tccattatcc
     1081 aagaaaaagg aaattgtaca tcaatcatat tcagattttc taccctgata ttttcgatca
     1141 ctccccctct ttcttttgaa gatttcattc gaataccaca gtctgactga aaaaaatcga
     1201 tatcgtgaat atatacgtca ctaactccag cactaacttc gctaccaata gttacaccgt
     1261 atcctgaacg gattttacat ctagcaacct cgattcttga tgattttcga ttgactcttg
     1321 caccattgcc atctctgcca gacttaatag caatacaatc atcaccacag gccaaatcac
     1381 attcatagac acgcacattt gttgaagaat caatatcaat cccatctgta cttggtccat
     1441 cattgttgcg gatgattact ttttctacta atacatcgtt agaataagtg atttgcaagt
     1501 tccaaaaacc tgatttctga aaaatcaaat cacgaacaac aacatgttct gcatgataca
     1561 gtaaacaagc gcgtggacgt ttaatagcat aatccgcaat ccaacgaagt cccttttgat
     1621 cgtattcagc tcttgtcccg cttttttgat cttttcccca atacagctcc caccaatggt
     1681 ctccttgacc gtcgattatt ccttttcctt ctataagaat atctttcgcc gagagaacat
     1741 tcaaaatcgc tgctggccat ttcatttcta cgccggctac tcgtgtatcg atttctggaa
     1801 atgcttcgat atctgttctc cccttgagga ctgcaccttc ttcaaagata agatgactct
     1861 tatcttttaa aaaaagggct ccgactaaat aaatgccttt tggaataaat acaggtaagt
     1921 gttctttcgc accttgatcg attgcttttt gcagtattat tgtatcatct tgttgagtgt
     1981 cgtttgtttt aaagttcaaa gcattgataa tccctttttt tgtccgcaat acgtttctcc
     2041 ctatctttca ttgatttttt tcaattcttc accaataaat acagtttttg aacgattcag
     2101 tgggtaacct actagcaaaa tgattgcgga tacaaataag cagaatgctg gtaacaagtt
     2161 tgccatcgta taaatccttt cttgtactaa aactgattgg atggctccac ctgttgtaga
     2221 cgattggtaa ccaatgagtg ccagcataaa tccgcctacc gctcctgcac atgcttgtcc
     2281 tacttttctc gcaaaagaat ttactccata tactgttcca tcttctctta agcctgtctt
     2341 atattgatga taatcaatca cgtcagtaat gaatgcccag accatcaaat tgaataaacc
     2401 agatcctaaa gtagcgataa ataaaagaat cagatatacc catgcatttg ttatatgaag
     2461 gaaatacatc agcaagtaca tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt
     2521 tttaccaaat aattttgtca gctttgtagc aaatggtgct aaaactacta ctgttccgta
     2581 agtaaataaa agagcaacag acattgcttg tttattatgg aagtaatcat tgaataaata
     2641 agtcatattc gtcccagcta aaatctgatt gatcacaatg aaaatgtcaa caatgactaa
     2701 aataatcagt gctcgattag ctaacaaacc ttttaccaac tgagaagcag atactttttc
     2761 gtttttgaca actcttactc tttcagtagt caatttacaa gttaacatat atgcgacaaa
     2821 tgctattgca gcacatccga ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg
     2881 tccactcttg ttatgggtat acattaaaat cggaatgaag aatcctgtag ttgatccgcc
     2941 aatcgcagaa ccaatgcttc ggaacgtgga caacgaggtg cggtcattag gatttgagct
     3001 aattgctgct gccattgctc catacgggat attgattgtt gagagaaaaa tcccataaac
     3061 aatgtatgaa acaaaaatat aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg
     3121 caaaaacaaa atgacagtca ctaagcaaaa tggatacttc atttttctga tccaaggaat
     3181 aaatcggcca gtttctgtta actctgagac atcacataat cttccaacca tcacatcagc
     3241 aaacgcatcc aaaattctcg aaatcaagaa taacgtacct acgattgccc ctgaaacgcc
     3301 aagaacattc gtataataaa tcatcaagaa actattgact aaacctaaaa taaaacagtt
     3361 acctagatca ccgaacatgt agcctatctt atcggaaatc ccaaattcct ttttttcttt
     3421 ttttattgta tttgctgtaa cagcattttg tgaaagcatt tgaattcctc ctacctcttc
     3481 tagtaaacgt tttcaaaatt ataactcttt ttttcttgtt tttatattca caatgttggc
     3541 tttttattgc attatcttga cagataaaaa acaaaaaata atctaaaaac attgatgcat
     3601 aagattgata acttttttta cataacaata tatacaaaca aaaaaattca ctaactagtg
     3661 aaaataagtt agtgaacctc atttattaaa gaattaaatt acagatgatc ttttccatca
     3721 tacttgaaaa actcaaaatc agcataactt cctctttttt tatttagatc atgcactgct
     3781 atcccgataa aatttccagt aaaccctcct gagagtatac acatgttttt ttgcggacct
     3841 actttcttcc attcttcagt tgatacgtct ttcaaatgaa actgtgctgt ctctttcttt
     3901 acagtgactt tcaaaaaagt cttcttttct gtgagtggaa tcgtccaatc ttctatttgt
     3961 gctactccgt tttctgattc tatcatacgt aggcattttc ccttgtcctc attccaagta
     4021 acataacaat aaatataatt ggattcattt aagtacagta ccagtccagc catctgatta
     4081 aaattattag gcatgtattc aaatgctgtc tccgcatcaa aagtaaaatc cgtttgccga
     4141 atagctaaca aatgatgttc gaaggtagat tgaagagatt caccggaaat cagacgtaaa
     4201 aaacctggtc tacattttgt atcacaccaa tcagcatttg gcaaaatgcg tctggcattc
     4261 cagttcttct ttagctctcc ttcaaatgta tccataaact gatgatcttt ttcttgttgt
     4321 acctcttcat ttgtctcaat cattatttct tccatcggag ttgtctggct atctttaagt
     4381 cttaaccaat cgtcggacgt ccaatacact tcttgaatag cggtttctct tcccaaaata
     4441 gccgcaccgt ttaatggtct ggtacaaaga tatgtcatgt accatcttcc tgacggggtt
     4501 tgtactaagc tcccatgtcc tgtacattgc aatgtagaag caggtttgtc actagctgtc
     4561 agcataggaa aaccaggatc aagctcgtaa ggaccagtaa tattctttgc tcggcaaact
     4621 gtaactgaat gtcctcttcc tgtaccgcct tctgccgtca tcaaataata atacccgtta
     4681 cgaaaataca aatgtggtgc ctccgttttc gctaattctg tacctgcaaa tattttatag
     4741 atgggaccat caagcttctt cttctctgca tcatattctt gtaatacgat accgctggat
     4801 ttattcggtg tgtccagacg ataatcccat agttcattga caagccattt ttttcccgtt
     4861 tcaagatcat ggaacaaaga aggatcaaag ccgttactat ttaaataaat tggttcggac
     4921 caaggacctg tgatctcaga tgcagtgatc aaataattgt ggacatcttt aaacggacgg
     4981 cctgcacttt tgacatctgt gtaaacaaga taaaacaatc catcagcata actgatctgt
     5041 ggcgcccata tacttccatt tgtaggattt ccttgcaaag atacttgagt ggtcaaaata
     5101 tccgttgtat gtgaccagtt gactaaattt ttcgactcat atactcggat tcctggaagc
     5161 cattcaaaag aggagacaat aatgtaatac cgctctttca ctcttaatat attaggatct
     5221 ggattgaatc ctcttaaaat aggatttttc actttgatag tctgcatgat tcaggctcct
     5281 tttctcattc ttactcgata taaactgaat gccccagctg cgaaataaaa aaatgcaaaa
     5341 ggaaacgcaa taaactgagt gccaatcaac aatatgaatc ctgttataaa atacaaacca
     5401 gtggcagttg ttaagtattt tcctttagca gtcaaaaaca atgctactgc taaaaaagca
     5461 agtgaaataa acaacacaac ggcaaacaga ccagtcatgt cctgtaataa tccaaatgta
     5521 ttttctaaag acaaagttga atgatctaca gcaagcgact gataactttt tttaaactct
     5581 gacgcagaca tattacttaa agtaactgct aacccgccaa agaaaatcaa agatagactg
     5641 ccaccgacca ttcctagcaa tagttcccat tttcttttca tttctgaacc ccctgctttt
     5701 ctaaatgtat ccattcaggt cttttgatcg atgtttcata attgttatta cgttcagaga
     5761 atttgacagt ctgttcattt ttcttgtcat cccaattatc ccaaccagtt gggtgaacat
     5821 gcccacctat cttacacgct tcaaataaga catttgcata cggacgccac ggtcttccta
     5881 aatagacgtt tctagtatca ggttctgcag tcaaggtaca gctttcaaaa cgataccctg
     5941 ttttttgatt tttcggcgta gatgctgctg tgatgtatcc aggattttta ctggctaagc
     6001 tatggatatg acactgatga aaaacggcat ctgccccacc aaatatgaaa tccaccgttc
     6061 cttcaatcca gcaatattca taggtctgac gacaatactc aggattggca gaagccggtg
     6121 tacaaaaagg agtaccatct ttttgcaaat caggcaacgg acccgtgcat aaggtatcct
     6181 gataaccaga tagtcgacag ttttttaaag atacgcgatc tcctgaattg aataaagcaa
     6241 cagcctgtcc taccttttct cctggacctg ctgtattttc gatgtgaaga ttttctaata
     6301 cgacattcga tccttctaag aaaaaagtag cacttcggaa agtccctaac tggctaccgt
     6361 caagatactc ctgttttgca aaacgattac cggagataac tactgttcct gctcccacaa
     6421 gtttaaaatt ggataatctg ctttctatat attcatcata tcttccttct tttactatca
     6481 agacctttgg atggctatcc ggcaggcctg ccaaataatc gattccttct tggatagttt
     6541 caaaatccgt cggggtatct atagcattct ttccaattat cagtttgtct ttcatcctca
     6601 taaatgctcc cttatgaatt gtttccaaat atttctcatt tcttgtgttt ccatatgacc
     6661 gcctgttaaa gaaaaagaag aaaagttttc tggactaccc attgattgat actttttgtc
     6721 cagccctttt ctcagcttca gtacgccttc gtccaagcac atcgtatcgt tttttccaac
     6781 taagctaaga cgagggcgcg gagcgatttt ttcttggatt gctaacgttg tataagctgt
     6841 caatagattt ggcacatagt aataaaaccc atgatgatct aggcggcgat gtagcaaaag
     6901 tatttcgatg tccacctgtg ccgctagatc aatacaaact ttgacacgag aatccaatgc
     6961 tgtcacccac cagcttaata agcctcccat agaaagacca atcgttgcga gccgttgagt
     7021 atctgtgtct tcccttgtct ccaaataatc gatcaagctg atcacatcat aaatacgcat
     7081 tccccataac gtttttcctg ttaataaaaa ttgtttgaac aactcgcttt cagaaatacc
     7141 tccgcgttct tcaaatcccc aagcatcaat cgaccaaaca gcatatccca ttcctgtcag
     7201 tacttctgca aaagatggag aagctaagta aggagcacca tggatcaatt cacttttccc
     7261 gactgaaaaa tctccgccgt gagaatgcag gtaaaggacc ggcggaaacg gtccttcttt
     7321 ttctggataa gcatagaggg ctggtactgt ttccaattca tttaagtaca attgatagta
     7381 ttcaagcata aaaccgtttt gtttttctcg gtataatcgt tttccactag gggtatgtct
     7441 gctagatgga gcacctaaaa aatcattcaa ctgcataagc aaagttttta cgaacaacca
     7501 gttcatccat attttctgat tgatttgatt ggaatttctc aatactgatt tctttagtat
     7561 tttctaatag aaatgctggt ccctcgtgat tttcaatcga catctggtta aaggaaacct
     7621 tacccgcaaa gccaatataa aatccttgtc tgctcatttc ctctattcct gtcatcatag
     7681 caggaacacc gggaactgca tcttgggcca tcgagacatc gatattgtca aatgtgattt
     7741 cttgtatgaa ttgttccgct aatccataaa taaaacctgc agaagcatgg acattccttg
     7801 ccgtgatatt cgaaaaatgg atccttctga atgctggtgt ccgttcatct atcggataag
     7861 cctttttttc ccatacatac ggttctttcc ctcgcggacc acagaaataa taaagattca
     7921 gtataaatgg acacatcaca ttatccatga ctatattgga tactcgaata tcttcaacga
     7981 tcccgcctcg acctcgtcta gatttcaatc gtattcctcg atcagtttct tggaagatac
     8041 aatttgaaat ggtaatatta cgaatactac cactcatttc acttcctaat acgacaccgc
     8101 catgtccatg gaccatcgtg caatttgtga tcgtgatatt ttcgcaagcg atcctttcgt
     8161 acgtatcttc agtccctgcc ttgattgcaa tacagtcatc tcccacgtca atatggcaat
     8221 tactaatacg cacatttttg catgattctg gatcaattcc gtctgtattt ggtgaatccg
     8281 ctgggttcaa gatcgtcaag ttatcaaacg ttgcattact gcaaagaatc gggttgatcg
     8341 tccaacttgg tgactggatc aatttaatat ctttcacagt aattcgatga cagttatgga
     8401 aactcatcaa tttaggacga ggataagcga gattatctgg ttcatttcta aaagtatgcc
     8461 accatttttt tccatttcca tctaatgtac cgaaaccagt gacagagatg ttctctacat
     8521 tttgagcata gatacaggaa gcataaactt ttcgatggac tccttcccaa cgtgaatgaa
     8581 caacaggata atcttttgga tcgtcagaga atttcagaac cgctcctgcg cttaaatgaa
     8641 gttcgacatt gctttttaaa aataaagctc ctgttagaaa ttcacctgct ggaacaacta
     8701 cggtcccgcc tccgtcactt gctgcagcat caatggcttg ttgtattgct tcagtattca
     8761 attcatcaat tgatgcgcca aatgtcaaaa tatcatacat atttataatg cctccacttc
     8821 aatcaatgct tgcaagaatg caccaacgcc tttttgatca ttacaaataa ttggttcact
     8881 gatgtagtaa gcatatgaac catctcgctg atctgctccg cctaaccctg ctacttgaca
     8941 gtttttattc aagttcagcc agccttcttt tgtttccaga acaaattcat caagtaatcc
     9001 ttgatgtgat ttcttcactt gtggaagcca ttcttctttg ttcagcagtc ccaaacgtat
     9061 tcctttagcc attgcgtaaa caaacatact actgccggaa gcttccagat aatttccttt
     9121 ccgttttgtt tctgttgtta cttgatacca tgtcccgctg ttttcttctt gatatggaag
     9181 taatgcagcc agtgtctttt ctagaatttc tttcagcaaa gaacaatctg tttcttcatg
     9241 aagtaattca taggtatctg ctgcagccat caaataccag ccaatcgatc ggctccaaaa
     9301 atgatgggat agacctgttt ttggatcagc ccatggttgg accttctttt catcccatgc
     9361 atgaatcagc aaatttgttt ttggatcgaa tagatgttta taactgattt caaactgtct
     9421 caaaacatct tggtaatctt tttcttcact aaaattctgg ataaattcag cataaaaagg
     9481 agaacccata tataagccat ccaaccaaat ttgataagga taaatttctt tatgccaaaa
     9541 agctccttcg gatgtgcgtg gatggtttgc caactgctgg gaaaggagtt ctgctgcttt
     9601 tttatatttt tcttcgcctg tttctttgta caataaaaag aaaagctttc ctgtatttac
     9661 atggtcgata ttgtattctt caacgctgta gccacggatc gttccatctt cttgcacgaa
     9721 atagtcgata ttgtctttta tgaaatcaaa ataaattttt tcttgggttc gttcccaaag
     9781 taattggaac cctttaagta cgacgccata gtcataagac cattttcctt tataccactt
     9841 ttcttcatat aagcgaggtg ttctttccat gatacttttt gctaatttct ttgaccagtt
     9901 ttccattatt tgctctcctt taaaaattaa aataggattt gggacaaata ccaagaagta
     9961 tttgtcccaa tcgtctatat tactgtgccg ctgctttgta tgcttcctcg tactcttgtg
    10021 tgattcgatc gcctccttgt gctttccagt cttctacagc ttttttgaat ccatcttcgt
    10081 caagctgtcc catgatgtat tgcaccgtag catcaatgac gattttttca atttctgatc
    10141 ctacttcatt gttcgtcggt gaatccaatg gcacagaagg atccattaca gcaaattctt
    10201 cattttcttt gatcaattga ttagagaatt ctttttcagg gtctgtatct ttgaaagaga
    10261 acgtcacttc actaggacga gagcttgata acggttgcac ctcttgttgc caaagatcca
    10321 tatcgataaa ttcgatagaa ccatcatcat tcaattcata atgcgttcct tcgataccgc
    10381 cagtcatcaa tttatacaca tcttcttcta aaagatcatc aacaaattga agtaatttct
    10441 tcaattgtgc ttcgtctttt acttgagatt tgggaaatgc tagcaagccg ccaataccat
    10501 ttccttctga ccaaacatga taaccgccgc cttcaccatc tgagatcttg tttacaggca
    10561 ctaattccat atcatcttga ataccttgac ccatcgtctt cagattcttg atatccacca
    10621 ttccagtgta gattccagtt cttccttgtg caaattgttc ttgctgatca gtcttttggg
    10681 taactgcaaa gtcttgtgcc aaatagccat tctcaaatag atctcttgaa aaattcaaag
    10741 ctttcatata ctcttctgta tcaaattctg gagtgaattt gccattatcg tctacagccc
    10801 agccatttgg tgctccaaaa taagaagtga aaagtttaaa gctggtataa cgaatatcac
    10861 taggcgcacg atcgccaaaa ccaactgtgt cgtccacacc atttccatca ggatcgtttt
    10921 ctgtaaattg tttagctact tcataaagct catccatggt agttggaaca tctagtccta
    10981 gattatccaa ccagtctttt cgaatgacta agccagcacg agcgtagtct ttttgtatcg
    11041 gtacgccgta caaggcaccg tcgatactcg cagcttcgat tctatcttca gaaatttgac
    11101 ttaaattttt atagtctttt aaatattctg atacatccca gaatacgcct gattttaacg
    11161 cattgcggac agaagagttt ttcaacatcg caatttgaag tgtaacgata tctcctagtt
    11221 catcagaagc caatgcagtt gttaatcttt cttcttttga agcatcagga atccagttga
    11281 atgtcagttc cgtatcggtt ttttcttcga tcaagttcac aatcgtgtct gtaggcggtg
    11341 aagcatgatg caaaatattt aaccaagtga ttttagcttt tccatcttct gtctctgaag
    11401 aactgttact tgaactgctg ccgcatgcgg caagtgctag aattcctgca aaactaagac
    11461 ctaattttac gagcgttgct tttttcataa attttctcct tcactgtttt aataatattt
    11521 ttgttttctt ctgttttttc tgtatatgag ttcttccatt ccaaatggca aaatatagaa
    11581 tttgctgtcc acgtgactgc ggaaaagaag agactgccac cgataaaggt taaaacacct
    11641 ggcattgttt ggatgatcat cagataaatc cctaccatac ttaatatcaa cactaatgag
    11701 taatgaagat gtgagaatcc gaataaaaat gccattttca ttttgaaaaa atatcctttc
    11761 caatcataat gagccatgat tgggaaaata tagagtgctg agagcacata tatgaacgca
    11821 gcaatataca aagcgatcct aagataccaa ttgtgtatga aagaaagatc cacaagtaaa
    11881 acaatcccga tggcgccata tatccagctc atcagcaagg tttcacgata gttttcttta
    11941 aaatatgtga agtacgtttt cgtcaaagga aacgtttctt ttgagcgcag ccattttcta
    12001 atcactgcaa ccatcgcata cgatgcagga ccgaaagtaa aaactcccaa acctgcgatg
    12061 actaaaccgc cccacagaaa attcaaataa accaactgta aaatacgatt tagccttccg
    12121 tttattttat ctaatgactg cagcatatct ttccctctct ttctatctta gtaaacgccg
    12181 tcttctccta gctttttggc aatcttatta gctaaaatta ccataaacaa tcctacgacc
    12241 cctttaaaca atcccattgc tgtactgaaa cttaactgtc cattcttcaa tccagctgta
    12301 taaatatagg tgtcgaaaat ttctgcaacg cttcgattca atgaattcaa taacaagtac
    12361 atatgctcga accctaagtc taatgtctga ccgattttta gaatcagcaa ggtaatgatt
    12421 actggacgaa ttgccggcaa tgtaacatgc catgttttgc gtaagcgtcc tgctccatcc
    12481 atttctgcag cttcatacaa ctgtgtatcg actgcagtta tcgctgaaag gtagatgatc
    12541 gtagaccagc ccaactcttt ccagatcact tgtccgatat atactgtgcg tgtccattca
    12601 ggggatgtta gaaaactgat tttttcaaat cccattgttg caaggaattc attcaatacc
    12661 cctccgtcaa cattcaaaaa tacataagta atcgaaacaa tgattaccca agacatgaag
    12721 tgagggatgt aaatgatcgt ttgaatcccg tttttcaatc gtttgttcgt tacttcattt
    12781 aatagtaaag acaaaatgat tggcatcgga aaaaacacga agatatttaa tccgaacaag
    12841 atcaacgtat ttcttaataa tgtaaagaat gtcggttctg taaacaaacg aatgaaatgt
    12901 ttgaaaccga cccaaggact tcccattatt cctaaaaatg gttgataatc ttgaaaagca
    12961 ataacgagtc cccccatagg aagataccta aaaatcaaga aataaaggac tcccggcaga
    13021 atcatcacat ataaaaattt attcgttttg attcctgcaa ttattttttt tcgtcttctg
    13081 agcttttctt cttttttcga tagcgtcagt tgaaatggtt cttgtttctt tacactttcc
    13141 ataagcccct ccttagtttt gtgaatgttt gctgcttatg acttcatgat agataactat
    13201 ctgaattacg tatataatca tttttaccgt aagtaaaaac gcttacatag caatgatttt
    13261 tgtgctatac aaaacgataa agtgtatttt ttcacaaata aactacgcat agcaaacctt
    13321 ctattttttt ctatctgaca cttagcatag gaaatgatta tatacgtaat ctttcaccga
    13381 tgataaattg aacatataga aacagaaaca tttaatttat tttgaggagg agcctatcta
    13441 tatgaaaaag aaagtctcta taggagaaag aagttttaca cttttcaaca atacgtttct
    13501 aattttgcta gctttgatct gtatcgttcc ttttttaaat atcatcgcta catcttttgc
    13561 ttctacacaa gaagtcgtcg cgaaaaagtt tatccttttt cctactacct tttctttaga
    13621 cgcgtatcgc tacattcttt ctacgccgac catttttaga gcacttgctg tttcgattgg
    13681 tgttacaggt gtcggaacga tcgtcagtat gtgtgcaact tcactgatgg cctacggtct
    13741 ttccagaaag tatttgtttg gtcgaggttt cgtcaacttt cttgttgtat tttctatgtt
    13801 gttcagcggg gggatgattc caacgttctt agttgttcgt tctctagggc tggtcaattc
    13861 ttactgggcc atgatcctgc ctgtggctgt taatgcaatg aatatgatca ttatgagaaa
    13921 ctttttccaa gccttgccgg atagtttgga agaatcagca aaaatggatg gttgcacaga
    13981 ttttggtgta tttttcaaaa ttatgctgcc attagcgtta ccgtctattg ctactatctc
    14041 gcttttttat gctgttactt actggaacac ttatatgact gctattttgt atatcaacga
    14101 ttcatctaaa tggccgatac agattctttt acgccaaatc gtcattgtat caagtgggat
    14161 gcaagcagaa agtagtgccg ttgatgtgat tccaccagca caaacagtta aaatggctgt
    14221 tatcgttatt gcaacagtgc ctatgctgat tgtttatcct tttgttcaga aatattttgt
    14281 caaaggagca ctcgtaggtt ctgtcaaagg gtgattatgt actgaacgca aaatggtagt
    14341 tgtgtcagaa gtggtcagct tcaagaaata agaaggcatt tacgaaaatt gcttcttaaa
    14401 tttttgtgaa atttcggctt atttttcgaa gaatctactc ctggaacacc gtgtatcaga
    14461 ggttgtgaaa gagacgttca gccttgagcg atgataaaaa ctctcacaaa cattgcgacg
    14521 agtaacccac aggagcaata agaagcgatt ttgaaaaata gcttcccata tttcacaaaa
    14581 ttatgcctta ttgtcgatag cctgtctctt gaacgccatt tattcgtaaa aaagaactgt
    14641 gacaagactt ttgtcacagt tctttttgcg tgcttagcct tgaaatttaa ctatccataa
    14701 atacaaatac tagggttatt ctaacttctt ttattgcttg aatcagtcca caagaaaaac
    14761 agcctcttga ttcacaaata tttttttcga tattctcccg gcgtaatcga ttccttcttt
    14821 ttaaagaaac gaataaaatt ttgcggattt cgatattgca atctttctgc aatctctttt
    14881 acggagaggt tagtctcttt caaccatttt tttgctacct ctaaacgatg attttgaaca
    14941 aaatcaccaa atttttctcc tgtttccttt ttaaatacat tacttaaata gttagggtta
    15001 taatgcaacc tatcagcgat cacatctaaa gacagatcac gatcatattc tgtgtagacg
    15061 atatgcataa tattctccga caaattttta aattcatgat cagtgattgc ttgagagcat
    15121 tctgtgattg gcagaactaa ttgttctaaa acaagttttt ctagttcttt gggatgataa
    15181 ttgttcagtg cttttacata aatttgtttc atgttatcaa agttttttga atctactccc
    15241 aataactgtc ctaattgaac gagttcattg atcaagcgga ccgaaactac ttcccgactc
    15301 aaaggatttt tattttgtgc aaacaatgca tcaatcaatt catgtactaa attcttcact
    15361 tcctgatttc cagaacgaat ggcttcaaac aatcgatttt gttcttctat cggatatcta
    15421 ataagtgttt tttcatgagc agcaggaacg atttcctgat aaaaaataat tgaacttgga
    15481 ccagtattta cacgataata cagggcttct tttgcccgat caaccgattg cttgctttca
    15541 ttcagcgttt cgaaccggtt actgatgcct atactgatag ttaaattcaa gtattctttt
    15601 acacgttttt gaatgagtcg gcaatactcc atgacttttt tgcttgcttc tgcatcatct
    15661 ttatccattc gataaatagt ggcttgcatt tcttcattca aaacgatcgg cagcattcga
    15721 ttttcctttg gcacaatttc cgtgatcatg ttgttgatcg ctaaaagtag caaatcacgc
    15781 tccccaccgt gactatcttc taacagatcg atttgaacca aagctgtata atagcagtca
    15841 ttttgagaac tataaccaaa ttgctgcaac cgctgatcta gttctcgcct ttctactcga
    15901 tttctaaata aactaagtac aaatagcgtt tccagctgtg gcttttgggt aactaagtgt
    15961 gcacttaacg cttctttttc tcctatgatc cgactgactg attgagcgac caaatctagt
    16021 tctttcccac tgaatccttt tgatttaaga ttcagacgtt cctgtatttg agaaatcgga
    16081 cgggcaaatc tgtccgaaaa taagtaggaa agaagaccaa tgagtcctac cagtgttaaa
    16141 gacactgcaa ctagcccgat tctcagattg cggatcattg cgccaattgc actagagtca
    16201 acttctaatc tgtaaatcca attattataa tctgatttca gtactatata cgttttatct
    16261 ttttctctta tcactttcag ctgattgtaa tctagatcca cgtccgttaa tacagttggt
    16321 tgctctttat tttttactgt ctgagtagta aaaaggttgc caacttcgtt ttcaatagaa
    16381 agtatgtctt ctccactttc accaacaact tgttctattg tatgtttctc tatcgtagca
    16441 attcccaatg catatttttc tttttcaaac attggcaaag aaatcaccat ttcgattcct
    16501 ttgtttttct tttgccaaaa taaattattt ttgttattta tgtacttctg ttgatacttt
    16561 tcaatttcct cttcggtcag ttgactcaac gatccattga ttactcccca tttaccagcc
    16621 aaactgacta aatcataatt attatcatcc ataacaatcg tttctataaa atttagttct
    16681 ttcctgattt cagaataagt ggagtaatct gtatatgtca gcggatgctt gaatatgtcc
    16741 gagaaatttt tggtcgaacc atacgtagaa aacgaatatt caatcgttct aattttccat
    16801 tcaacgttag ttttaacttg cctgagcaaa ttctttttgt ctgttaaata actcgattcg
    16861 atcgattcat aggtattctg gtaaatgaat aaactaaatc ctatgacaag tacggctcct
    16921 aataaaaaca tcggtaaaaa tattttataa aatactgtac ttttcttcat tttcttcctc
    16981 tcctcctttt aaaataaaag gctttcattt ttctgtaaaa ttgatactca tctaattaaa
    17041 tgagcattcc ccgctttcaa gaatagcata attaaaaata aaaatccaag taatttttaa
    17101 cgtgtgcaat cttttcatat caaagatcta ttgacttatc gtagttatcg taatatgatg
    17161 aatgcgttaa caaaaagtga aaaagtaagg aaaatgatgg aatggtaacg atcaaagatg
    17221 tcgctaaagc agctggcgtt tctcatacga cagtttctcg tgctttgaat aacagcagtt
    17281 taataaaaaa tgaaacaaaa caaaaaatac gacaactagc taaggaaatg aattattcgc
    17341 caaactatag tgcaaaaggt ttagtgaatc aaaaaaaata tctaatcgga ctatttttct
    17401 caagtttgca taaaggaact tcatcaaatt tcctagcaga ttccattgca ggcatccatt
    17461 cagtattaga cacaaattac agtttatctg ttgaaagtat cgatgttgtt gatttaacag
    17521 aaatcaacct gcaacgctac gatggactga tcgttatgag tcaaagtgat gatgatcagc
    17581 ctttcatcga ctatctcaaa gaaaaagaat ttcctttcgt tgttgtcaat cgtcacatca
    17641 atgatccagc ggttatcaac gtcactgccg atgatgcggc cggtgttatg caggcagtag
    17701 attacggtat tggatgcgga catacgaaga tcgcttatat tggcggaaag aaaaattttc
    17761 gttcgacaaa agaacgggaa aaaggggtat tgaaaagtct aaaaaaagcg agcatccctg
    17821 tcaatccagc ctatttttta acaggagatt acagcttaga aagcggcctc caaaacatgg
    17881 aatacctatt atcactttct gacctgccta ctctggtttt ttgcggcaac gacgacatag
    17941 cgattggtgc attgagagct gtacatttag ctggcctccg tgttccccag aacatttctc
    18001 tgatcgggtt cgatgattct ccagtcgtct cctatctcaa tccgccgctt accaccgtac
    18061 gtaagccttt gaaagaaatg tgtcaagaag gaacaaagct tttactagat ttgatcgaag
    18121 gcaggacgat cgaacaatct aaaattgagc tctccacctt gttgatcctt cgtgagtcga
    18181 tcaattactt gtaaactaca agtaatttta tataaaaatg ttaacgtgtg cataacacag
    18241 aaaggaagtt tatatgaaaa aactgacaaa agaattagtg aaaaaaaacg aagcacctgt
    18301 aaaagttctt cagtttggtg aaggcaattt tatgcgtgga tttattgatt ggcagattca
    18361 gcagctgaat aaccaacaat tgtttaaagg taatgttgca atcattcaac ctttagaaca
    18421 aggtcttgga aatatgatga accagcaaga taatttatat acggtcattt tacaaggact
    18481 cagcaatggc gaaatcgttg atacttccga gattatcacc gtagtagaac aaatgatcaa
    18541 tccttatgat gaatgggatg cttatctatc tctagcagaa aatgacgact tggcctatat
    18601 cgtctccaat acaacagagg caggaatcca attcgtggag actgataatt taacagatac
    18661 tcccccacag agcctcccgg gcaaactgac agccttttta taccgacgtt tcaaattaaa
    18721 caaagctggc tttaccatca ttccttgcga actgattgac agaaacggcg aacgtctaaa
    18781 agaaatcatt ttacgctacg cagaaaaatg ggatttagaa cctgcttttg ttgagtggtt
    18841 agaaaaagaa aatgttttct gttgtagttt agttgatcgc atcgtcccag gttatcctcg
    18901 cgaagatgca ggtgttttca atcaacggca taactatgaa gatcaattga tggtcaaagc
    18961 agaaccattc atgctttggg tgatcgaagg gcctaaagaa ttggagaatc aactaccatt
    19021 caaacaggct ggcttgaatg tcatcatcac agatgatatg actccttatc gtgaacgtaa
    19081 ggtgcatttg cttaatggac cgcatacagc gatggtccct cttgctttgt tggcggaatt
    19141 ggaaactgta gaagaagtaa tgaaagatcc tttattctcc gcatatgttg atcagttatt
    19201 caatctagaa ttgattccta tgctttcttt acctaaagat gaattagcca tttatgctga
    19261 ccaaatcaaa gaacgtttct tgaatccttt tgcccatcac aaattagaag cgatttcatt
    19321 aaatagtgtt tcaaagttca gcacccgtct gcttccagtg ttcaagaagt atatcgaaga
    19381 acaaaatcag gtaccaccgt taatcactgt ttcactagct gcgctacttc ttatgtatcg
    19441 aggcgatcaa gtaaagccac atgatgatga aaagacgata agcgaattta ctgatgcttg
    19501 gtctgataat ggaacagcta ttcctcgatt attgcaaaac gctgcgcttt ggggagaaga
    19561 tttgtctcaa ataccgaatg tgacagatac agttcaagaa atagctgcaa agatcgatca
    19621 aaaaggtgtc cggtcagtat tacaagaaat gaaaggaacg atggtccatg cttgaaaccc
    19681 tgcaaccaac aatgaaatta aatcgacgag acagtgtggt cgtcgcatta acccctattc
    19741 caaaacaaac acaactgacg attgatcagc aaacgatcac cactcttgaa gatatcccgc
    19801 aaggtcataa gattgcattg ggtgatttga aagctggaga caacgtgatc aaatatggct
    19861 atccaatcgg tcatgtgacg acagacgtaa ctgccggtca atggctgcac actcataatg
    19921 taaaaaccaa tctttctggt gaattggatt atcgatatga aaaagatgtc catccaagtc
    19981 attacctttt tgagaaccga acatttcaag gttacttgcg aaaaaatgga aaagtaggga
    20041 ttcgtaatga tttgttttta gtacctaccg ttgggtgtgt gaatggcatt gctgaactga
    20101 tcgtcaaaca gttcaaggaa aaacatccag atctgggaca gtttgaccat attacgattt
    20161 taaagcatcc ttatggctgt tcgcagcttg gaaaagatca tcaaaatact cgagaaattt
    20221 tagcggacgc agtaaatcat cccaatgccg gaggagtttt agtatttgga ttaggttgtg
    20281 agaacaatac agttcctgaa ttcaaaaaaa ttttaggtga atatgatgaa gaacgagtga
    20341 aatttctagt ggctcaagat gtttacgatg agatcgaaca aggtgtggct ctattagaag
    20401 agcttttaac agcggcggaa aatgaccagc gcatatcagt cccgctctct aagttgaatg
    20461 tcggtttgaa atgcggcggt tctgatggtc tatcgggaat aactgctaat cctttacttg
    20521 gtgcattttc tgactacttg attgctcaag gaggcagcac gatcttaaca gaagtgccag
    20581 aaatgtttgg cgcagaacaa gtattgatgg cacgagcgga aaatgaagaa gtttttgact
    20641 caatcgttga cttgatcaat gacttcaaac agtatttctt gtcttacggg gaaccagttt
    20701 acgaaaaccc atctcctggt aataaagcag gtgggatcac tacattagaa gataagtctt
    20761 taggctgcac gcaaaaatca ggaagctctc ctgtcgttga tgtgctacaa tatggcgaaa
    20821 aaatcagaaa accagggctt agtttattgc aagctcccgg gaatgatctt gtagctgcct
    20881 ctgctcttgc ttcatccgat tgccagttgg tcttgtttac cacaggaaga gggactccgt
    20941 ttggctcata tgtcccaacg gtcaaggtat ctacgaatac aactcttttt gaacgcaaag
    21001 gtcattggat ggattttaat gcaggcgtct tattagaaca accaatggaa gtaatcttag
    21061 aatcgtttgt tcaaaaaatc atcgctgtag ctagtggaga agaaacgaaa aatgaacaaa
    21121 atgacgttcg agaaattgcc atttttaaaa acggagtaac actttgattt tcataaaaca
    21181 gtataaaaag aaagaaggaa gatttatgtt tttatctgat gattttttat taaaagacga
    21241 ttgggcgaaa aagctttacc attcctacgc aaaaaacatg cccattattg attatcactg
    21301 tcacttagat ccaaaagaaa tttacgaaaa caacaatttc agcaatctga cagaagcttg
    21361 gttaggaggc gatcattaca aatggcgctt gatgcgagct tgcggcgtgc ctgaagaaaa
    21421 aatcacagga aatgcttctg attttgaaaa atttttagct tggtgtcaaa ccgtaccaaa
    21481 aattatcgga aatccgcttt acagttggtc acatcttgaa ttgaagcgtt tcttcggtat
    21541 tgacttgttg ataaatgaag aaaatgcaga gaaaatttgg acacaagcaa atgaaatttt
    21601 ggcagctcct gattttcgcc gtcgggaaat cgcaaaaaaa tcgaatgtag aagtgatttg
    21661 tacaacagat gatcccgcag acgacttgca ctatcatcag ttgcttgctc atgaagaacc
    21721 agatttgaag gttctacctt cattccgtcc agataaagca ctcaatattg aaaaagaagg
    21781 atttaatgca tgggtcacac tactggaaga agcagctgac caaacgatca ccacctatca
    21841 agaactgata gaagtcttag ggcagcggat agaatacttc catcaacatg gatgtcgtgt
    21901 ttccgaccac gctttagaca tcttacgcta taaagaagcg gatgaaacag tattagaaga
    21961 gatttttcaa aaagcacgag ctaacaaact attatcttca gatgaaatcg atgcttatcg
    22021 tacagaaaca ctgatacatt tgacccactt ctatcattct cataattgga cgatgcagct
    22081 gcacatccat gcttatcgta actgtaatac acaaatgttg gaaaaattag ggcccgatac
    22141 cggctatgat ggaatgaatg atctttcttt gactattcct ctgcaaaaat tattgaaccg
    22201 tgcagaagaa acggatcagt tgccaaagac gatcctttat tcattgaatc cgaatgacta
    22261 tcctgcgatt ttagctttga tgggctgttt ccaaaaagaa cagaatggaa aactgcagtt
    22321 aggttctgga tggtggtaca acgatactcg agctggtatg cgcgatcaat tgacacaatt
    22381 tgctgatggt agtgctttgg ggaattttgt cggcatgctg acagattcaa gaagtttcct
    22441 gtcctacact agacatgaat atttccgtcg tgttctttgt gaatttttag gcgaaatggt
    22501 catgcgagga gaagcacctg aagatgaaca acttttagga agtctagttc aagcgattag
    22561 ttatacaaat gctaaaaatt actttggctt ttttgcctaa ttgattaata gtaacagtaa
    22621 cgaatcaaaa acaggatagt gaaataacgg aatccgttta tccacgtttt agagaagaag
    22681 acggaccaaa tttgctccgt cttcttctct tgctgttctg cttcattgcc tataaacctt
    22741 tatgaaagaa gagataacat gcctactatc catattgccg gtgattctac tgcggcagaa
    22801 aaagatgaaa acaagcgtcc cgagacaggt tggggagaaa aaatctctgc ttatttttca
    22861 cccgagatcc agatcaataa ccaagcaaag aatggacgca gcaccaaatc atttatccag
    22921 gaaggaaggt tagcccgtct agcttcacag tttcagccga atgatttctt atttgtacaa
    22981 tttggacata acgaccagaa aatccaagaa gaaagaggta cgcttccctt cggtagctat
    23041 ttaaaaaatc tgcagatatt ttttgaatgc gcaaaaaaag cgaacgtcca aatggtctta
    23101 ctgacatctg tgacgcgacg agattactta gaaaacggaa cattgaatcc agatatactt
    23161 ggagactatc caaaagcgat gcgtgcattt gctgagaaac accatattcc tcttttagat
    23221 gtgttctcaa gatcacaaga attgttccag accttctcaa aagaagagac aaaaaaattc
    23281 tacctgcatc ttatacctga tacttgtaaa aattacccag aaggactaaa agacaatacc
    23341 cactttagtc cagaaggcgc tgataaagtt gcccgaataa ttgttgaatt gataaaagaa
    23401 tcccgtctgc ctcttgctaa ttatttacaa aaaggagttt gagatgtttc cattaaattt
    23461 aggcatccgt gcccacgatt tagatgttcg aaatcgtgaa gaaatcgttc aaaaagtatc
    23521 acagcttcac ttgtcccata tccaatttgc accacaaaaa gcatttcctg aacaacttaa
    23581 attaactgac atgacacttg ggagtgctgc ctattttggt gaatacttcc aaaaaaacaa
    23641 tatcgaactt tccattttag gatgctatat caacctatcc agcaaaaatc aagaagttcg
    23701 tgcacaagca ttggcaactt tcaaacagca aattgctctg tgctctgcct atcaagccaa
    23761 gatgattgct acggaaaccg gtagtgtctc tgtcggttac acaaaagaaa attttactga
    23821 agaagcttat cagatcgcca gaaattctat cattgaatta gtcgctcatg cagaaaattt
    23881 tggcgtaact gttgctgttg aagcagggat caatcatcca atctataact atcagttagc
    23941 taaacgttta atcgatgaag tagcttcacc aaatttaaaa atcattttag attgtgcgaa
    24001 tttgatgcat aaagaaaatc atgctgatca ggaaaaagtg atccgaggtg ccttggacaa
    24061 tttacatggt tatattacag cgcttcatct caaagactat atcatggaaa atggaaagat
    24121 ccgcatggta cctgttggca agggctgttt agatttccgc ccggtactgc attatatcaa
    24181 atatgagcgt cctctaatgt acgccactct tgaagctact cctgagattt atgttcctga
    24241 agcaatagaa cacttgcagt ttatttacca agattgttaa ggagaagatt atggaggaac
    24301 gaatacagaa aagttatcaa aaatatcatg caaatgcttt tcagacattg ctgcatcttt
    24361 atcagggaaa ttattggcgt tttgccatgt caggactctt ttttattatc aaacatcttc
    24421 catcttggtt aatgccgatc gctattgcca atgtaatcaa tgcagcttca tcgaaagatt
    24481 cttctcagtt gcaggccatt tatttcaacg cttttttgat ggcttgtctg attggtcaaa
    24541 atatattgac gaattatttt catgtaaaat accatagcac ttcgatccgt caagtagaag
    24601 ctggactaag agcgaccctt atcaagaaaa tccaagaact atctattcct tatcaaaaag
    24661 aattgcaatc tggacgaatc cagtcaaaga tcatccgtga tgtagaagct atccaaacgt
    24721 tgtcctctca agtatttgtc agcggattaa atatcgctgt caatttattc gtagctttag
    24781 gtatcacgct ttataaaagt cctgttgttt tcatgttttt cttattgact gttcctctcg
    24841 ccgcattcgt tactctcttt ttccgtaaaa ggatcagaca gtccaactca gacttcaggc
    24901 aagaaatgga agagacttct gcaaaagtga tcgaagtgat cgaattagca ccgattgctc
    24961 gagctcatgg gttagaaaaa cacgaaacaa ctcgcttaaa taagcatttt actcaaatct
    25021 atgaaaaagg attccactta gatatcctgc aatctgtttt cggatcagtc agctgggcta
    25081 gttttcagat atttcagctg atttgtttga ttttcactgg gatattagct ttacaagggc
    25141 gcatccaacc cggcgatgtc gtgatgtatc agacgtattt cacgacgatc gtcaattccg
    25201 tttctggaat gatcacgctg attcctacga tttcaaaagg catggaatct atcacttcta
    25261 ttggcgaact gttgattgct ggcgatgtag aaacttatca cggtaagaaa aaaatccaac
    25321 atttggatgg aaattttcgt tttgaggatg tttcttttct ttatccggac tcactcaaac
    25381 cgattatcga tcatttttct ctagaggtga aagctggaga aacaattgct tttgttgggc
    25441 catctggctc tggaaaatct acactcctta acttattgat tggatttatc cagccgactt
    25501 caggaaaaat ttttctagat gatcagcctt tggatgaact ggatatgcga agtgtgcgaa
    25561 attatttagc cgttgttcct caaaccactt tgttgttctc cgcttcgatc aaagagaaca
    25621 tcacttatgg cttgaaaaac gtatcaaaag aacgtttaga tgaagtcatc gaagctgcgc
    25681 aactttcttc actgattgac cagcttcctg atggtctgga taccttagta ggcgaacatg
    25741 gcaacaagct gtctggtggc caaaagcaac gtatctccat tgcacgagcg ttgatacgtc
    25801 agccaaaaat cattttgcta gatgaagcaa cctcagcttt agataaccag tcagagaaaa
    25861 aaatccagca agccctgaat tacctgacag aaacacccac tacttttatt gtggctcatc
    25921 ggctttcaac gatcaaagag gcagataaaa tcgttgtgat cgatgaagga aaaatcgttg
    25981 aaataggtac ttacgaaaaa ctgttagctc aaaaaggcta tttctaccgc ttgaaaaatg
    26041 aatgacaaaa aataggagct atgacaagac ttttgtcgta gctcctattt ctgaataaac
    26101 ggtgttctcc tgccaactcc tcggtcacaa gtcacaatag tcatcaaaca tgagaagctg
    26161 ttttttgaaa ttgttccttg taactcagag ttaaacggta ggctcacgac ctcttggtaa
    26221 atggtgttca agagacagcc tatcggcaat aagaagcaat tttgtcatcg ctcaaggccg
    26281 aacgtctctt tcacaccctc tgttgtttca taaaatagtt ttattcgctg taaaggtcgc
    26341 gaactggtgt catgatgata cggttcaagt cattgatgat tgtactgaag ccttgctctt
    26401 tttgcatcaa gtcattgatt acttcttctg attggatttt cacagccatt tcttgtgctt
    26461 tcattgcatc ttcgtcgctg aactcttgtc cactcatttg tttttcttgt aattcgattt
    26521 gcatcgcttg gaagtctttg aacagtttgt gtgcttcttc gtttgcttgt actttctcgt
    26581 acgcttcttt caacgctttg aattctggta attcacggat ctcacgttcg atttgattcg
    26641 cgctatcgta gatgtttgac atgttatgcc ctcttttctt ttagtagtta ctctctattg
    26701 taacatgggg ttattcgttt tcaaaggggc tactctcctt gatatttccg ccagaggtct
    26761 ttagccgctt caccaattcc ctgaacgcct tctttcacca tatcgccgaa gtttttcagc
    26821 ccttcttttg cttgttcacc atattgattc aagttatctt gccaatttcc agactctcca
    26881 ttagaatttc ctgtgtttcc aacttgatct gcagctacca cttgtcctcc tgtggcatag
    26941 gcatctgcta cactaaatcc tgtttttgaa gtatacggaa ggatgccttc tgccgctgat
    27001 ttgaagactt tcccgacaac atttccactt gtcccttcca aatagtgcag cttgctggat
    27061 tcttcgtaac ccatccatgt cgaaatcact acgtctggcg tgtaaccgat catccattgg
    27121 tcatttgctt ttgtggcatc gaaattagtt tctgtggttc ctgttttccc tgctatcgta
    27181 taacctgcag gattcgcagc agccgctgtt ccatttgaaa atgtaccaag aagcatgctg
    27241 gtcatttcat cggctgtttc ctttgatatg acctgttttg gttttttgtt tgtgttatcg
    27301 acgatcaccg ctccagtcga atcaacgatt ttcgtgataa gatgcggcgt atacatctga
    27361 ccatcattgg caaatgcact gtaagcaccc gccatgatca atggagatac tcctttttcc
    27421 agtccaccaa gggctaagcc ataatactta tcagattttg ctaaaggcag gccgaattct
    27481 tctgctttat catatccttt ttgtaaaccg atctcatgca aaagccatac agtaggcaaa
    27541 ttcaaacttt gtgcaacagc ttgatacatc ggtacttctc cttggtatgt cccgtcaaaa
    27601 ttatgcgcat cgtaataact ttgcggttcg tctttcaaga tactggaagg cttgtatcca
    27661 gcttccagtg ctggtgtata tacgcttaat ggtttgatcg ttgatcctgg tgaacgacgc
    27721 atctgtgtag caaagctgaa tcctctaaag acatgatctc ctcttcgtcc tactagtgct
    27781 tgtacaccac ctgtattcgg atctaaagct actgaagcag attggaccat ggcaccatct
    27841 gaagcatttg ctgggaataa tgcactgttt tgataagtag cttctaattg cttttgataa
    27901 tcctgatcta aagctgtata gattttatag cctttattca tgacatcttc ttcatcaaga
    27961 ccatactcgt taactgcttc atcgatcact gcatcaaaat aataaggata acgataacca
    28021 gaatctgaat tatcgtatgt gtcattaaga tacgcgctga tatctgtctg gctttctgct
    28081 tgttcttgtt cttttgtgat ttttccgttt tcttccatta cacttagtac tgtgtttttc
    28141 cgatcattcg cattttttgg gtgatcgatc ggattataaa taccaggacc tttcagcatt
    28201 cctgccaaag tagcagcctc agacagcgta acattcgttg catctactcc aaaatacttt
    28261 ctggaagcgt cttggacacc ccataccccg ttgccaaaat aggcattgtt aagatacatg
    28321 gtcaagattt cttctttgct gtacttcttt tcgatctcga ttgccaaaaa taattccttc
    28381 gcttttcgat caaacgtttg gtctaatgtc agatacgcat ttttggcaag ctgctgagtg
    28441 atcgtacttc ctccgccccc tgatgttccg ccagacaaga ccattcggac tgctgcacgg
    28501 gcaatccctt tgatatcaaa cccgtgatgc tcgtaaaaat tcctatcttc tgtcgagatt
    28561 actgcatctt ggatataggg agaaatcgcg tttaattcta catatgttcc tttttgggcg
    28621 tacagtgtgc cagcttcttc gtttttctta tcataaatga ctgtcgattg gcttaatcct
    28681 gattttaacg tttcgacatt tgcttgtttt gctaaaataa agaggtagat acttgtcact
    28741 aagacacaga tcaaaccaat cagcaaaatc aatttgttca catgatattt tttccagatg
    28801 cgctttcgcc atcgatggaa ttgtccgaga tatggtctga tccaacgcca aaaactgatc
    28861 aaagcagctt ttatcttttc taaaatgctt tgaaaatcca tttgcttctt ctcctgtcta
    28921 tttgattcaa tcgtcttatt ttaccatagt gaaagagtgt tttcttcctg cttaagttgt
    28981 agttcaatat gtttttttac gttagtgcaa gtagagtaac ataagaatga acagaaaaat
    29041 gtaagttttt tcttaaatat gttttaaaaa tggtacaatt agtctgcgat tgcactgaaa
    29101 ggagtacact tgtgaaaatc acgatcactt tacctatgaa ccatcctgaa acaacaattc
    29161 gcgacttgct tgaaaaggaa tggttagttc ctagaaaggt tcgacacttt ttgcgtatac
    29221 gtaaaaacgt ttggatcaat caacaacctg ctttgttcca tcatgaagta aagggcaatg
    29281 accagatcac attgttattc gaagaaacag attataatta tcaagaagtt caattaggag
    29341 atgcgaagca catccgtgtt ttatatgaag atgagcattt gattatagtg gataagcctg
    29401 caggaatgaa gactcatcca aatgaaccga cagaaaacag taccttactc aatcatttag
    29461 ctgcttatct cgacactaaa cagcaacgac cttacgttgt ccaccgcttg gacaaagaaa
    29521 caagcggtgc cgttttattt gcaaaaaatc catttgtctt gccgatactt ggaagaatgt
    29581 tggaacaaaa aatgatctat cggagatatc aagccatcgt atggggagaa attgccaaag
    29641 atatgaccat caccaataaa attggacgag atcgccatga tcggagaaaa agggtcgtag
    29701 atgataaaaa tggtcagagc gccttgacac atgtcactgt caacaaagta ctttctgaac
    29761 aaacacaagt ctattgtgtt ttggaaaccg gacgtacaca ccagatcaga gttcatttag
    29821 ctcataaggg acatccgatc gttggagacc ctctctacca aaatagaaca gctgaacgac
    29881 tgatgctcca tgcattagaa ctgcacatga tccatccatt tactcaagaa aaaatagtcg
    29941 gtaaagcttt acctggatta tgggaataga aataaaaaag aacagagtct tatcagcaga
    30001 taatgataag actctgttct ttttgtgtaa tttgtctgct tacttttgat gttcgtgttc
    30061 tgctaacagc aaactaccga taatcccact ttcattcggc agtccccaac gaacgatata
    30121 ttcacttact tgaggtgttt ccatataacc acccatcaat tcaacaaatt gctgacggat
    30181 tttttgaagg agatgatcct gattcattac cccaccgcct aaaataatct tttcaggtgc
    30241 taacgtcaac gtatagttga tcagtgcttg agcaatataa taagcctgaa tagcccaaac
    30301 aggatgatct tgcggcagat cttgcccttt gatccctgtg cgtgcctcaa tagacggtcc
    30361 tgctgctaat ccttctaaac aatctttatg ataaggacaa gtaccttcat aatggtcatc
    30421 tggatggcgt ttcacccaaa tatgtcccat ttcaggatgt gcgatccctg aaaaaatttc
    30481 ttcattgatc acgacacctg caccgattcc tgttcccacg gtaaggtaaa tacaactatt
    30541 ttttccttgt gctgctcctt gcttcaattc cccatatgca gcagcattga catccgttgt
    30601 ccaagcagca ggaatattgt agcgttcctt gattgtgcca agaaaatcga attgtgccca
    30661 cccttttttg ggagttgcca aaacgtatcc gtaattatct tttgttttgt cgataccaat
    30721 cgggccgaat gagccaatcc cgattccctt caattcataa cgatcaaaaa attcaaatac
    30781 ctgatccaat gtttcttctg gtgttgtggt agggatactt aatttttcta aaatttctcc
    30841 accatctgaa accgcacaca caaattttgt tccgcctgct tctattccgc catacattcg
    30901 ttttcctcct cgaatccgat tgtccgaatc tcataaggtt ggattattgg gacaaccgct
    30961 tgcttcgttg gttcttcaag cagattcaat atgacagttt ttccattgtc tttagttatt
    31021 tccaattttt ctaaatgact agacatattg aaacctctta ctactacttt atcactaaac
    31081 ttgctgcgtt tcaaagctgt aattgcaaac gtttcacttt ttatcgttaa atattgttgt
    31141 tttgaaatca atgttccttc atgatgtttg atttgttgag tggaaaaagg aatctgtgct
    31201 gcgtatgcat gcaaataagt agagaatttt tcttctggtc catgtagttc aatgctgtaa
    31261 ttgaaccgat gctcacctag acactgtgct tctggtgttg gaaaatatcc ccaatcgcct
    31321 agttctccca cactgcgaag tagcgtcact gcgatttgtc cgtcttctgt tacttcatat
    31381 tcattcaaac caaagtttcc gactgttaca ccgtactctt catcatgaat attgacaaat
    31441 gcttgctgat gttgcggatt cgtggggttt tcccaacttt tagacacatg gtttgggcgc
    31501 ttcaccactt caaaaatact atctgcttcg tgatgttcca catgtaactt tgtaggaaac
    31561 aagacacgta agcgatgatc tttcatctga ttgtcgagag tcgtttcaaa gtcgattttt
    31621 ttactatctt ttctgattgt catgattgtt tttatttgaa ggattcgttt ttctttggat
    31681 cgttctgctt ttcgataacg aaattccaca accatctgct gttctttttc taaacgctcg
    31741 tctgcagaaa cagggatttc catttcctgt agaatactta tttttgctat ttccggtgta
    31801 tctactaata cttttacttc agagttttct ttgttactgg ataggattgg ctgatcacag
    31861 aaaggttgtt taaagatata ctcatttcct atatcgccga tatcttcaaa aatatgcaga
    31921 ttattcaact ttcgtttgtt tttgcgatct tccattgtaa tggtaccatt tttttcaatc
    31981 gtcaaatgta gagggccgtt ttcgatgatt cgtccactct gatgaatcat tgtttccttt
    32041 tcttctgctt tcgcttcccc ttctacaagc gcgaatgttt cccaagaaaa tgctggcatg
    32101 ttttctaatg gtaaagtcac tttgatcatt cttgccatat atggcacacg gaaacggtct
    32161 ttcggcaaat cataaccaaa ttggacagtt tcttcagata aaatagctgg tatagtggcc
    32221 ccactttttg tttctacatg atacttacgt ttaggcaaat tttctaactc ttgatatagt
    32281 tgttcaggta ttccttcagc gaatcttttt ctttctaatt cgatggttat ttctgcttca
    32341 ccagttttat ccattcctgc agtatttaca atgacaaatg gaaagctgtc ttttgggaat
    32401 ccggtcgtat cgattgctgc ggtcagctgt tctaaggaat cttgtgcaag atactttcca
    32461 acttcatctg ctttttcaaa acgaggtatc atttcccgat ggacagaatc gactgaacaa
    32521 ccgcaaatac tgtcgtgagg atgattttgc attaatgtct tccaagcata atctaattga
    32581 tcatggggat agtttccact aacttcataa gccatcgtcg caagtggttc tgtaatattt
    32641 tctagctgtc gctggacctt ggtattccat tgttttaagt aaacccgagc agatgctgta
    32701 ttggctaaag tataccagcc atccgtttct tgacttgtca attctccttc tactgagcct
    32761 aagtcttctg gcacatcttt ttgaactgct tccagatagt cagtaaaatt agaatgaata
    32821 aactcgtaat ctggaaacag ttcatttgcg agatggattg cttttgatat atctttttgt
    32881 accggttgat gatcaacacc attcatcatc aataagtggt tcgtagaggc atattgttct
    32941 gcatccgcta atttttgctt ccaaaaggca agagctgctt ctttttctac tggaatctca
    33001 ttcccgttgc tataccaatt ggcaaatagc agaccaaaga ttgctgtttg atcaggtcct
    33061 ttccaccaca tctctgagta ttgggaagaa tagttttctg cttccaaaac ttgattatca
    33121 aagccaattg gttttacacc acggccaaac gctgctgctg aaataccagc ttgtttcatt
    33181 aattgaggcg tctgtcccat atttccaaat gtatcgggaa aatagcctaa catgacaggg
    33241 gttccccatt tacggctttc ttccatgcca atcagcatat ttctcacgtt cgattccgag
    33301 ctgataagaa aatcatcttg caagatataa aatggtccaa ttctcaattt tccttcattg
    33361 attgcttgtt ggattgcctg tcttttttct ggccttactt gaagatagtc atctaaaata
    33421 atcgtttgcc catccaaatg aaaactgttg aaggacggat caacttcaaa tagctctagt
    33481 agatcgtcta tcaattcaat cagtcgcatg tgatgctgtt cgtaagccat gtaccattca
    33541 cgatcccagt ggctatgaga aatgatatat actttctttt tcatttacga tacctcctac
    33601 ttttctactc taatatcaaa gtaatccatg actaattcgc aaaacatcat atttgcccaa
    33661 gaaaaccatt ctctcgtata attttcagga ttgttcacat cgaatccttc gtgcataagg
    33721 tgtgttcctg cgtcagttgc tacaagtaaa ttcaatatcc gctctttttc agatttgtct
    33781 gacgttgtca ttccttccat agctaatgca atcggccaaa cgtatttttc tggcgtatgg
    33841 gaggatccaa ttccttttgc gtactttcct tcatagaaat atggattttc tttactcaac
    33901 aatgttcttc ttgtttgcag ataatcttcc tcttgttcag aacaatatcc caagtaaggt
    33961 gcggcgatca gattgggcac attactgtca tccatgactg tggcgtttcc cattccatct
    34021 acttcatatg cataaatcgt ttgccctttt tgattaaccg ttcgtccgtg attctgaatc
    34081 ccctcttgga tctctttctt caatttctgt gtttgctgta caatttcttt atcatcgagt
    34141 atcgtttgaa aaatttcttc cacataacct aacacgacca ccgcaaacat attggatggt
    34201 accagatatc cataacggca agtatcatcg ctaggacgga aaccagacca tgtcattcct
    34261 gtaaaagctg tttttgcacc tcgcccgtca ttgatcagcg tatcttcttt tcttgtcgtg
    34321 tctcgtacaa atgtataagg agagtgttca tgatcttgct ctgttttgaa cacgtccaag
    34381 atttttttga taccttcaat aaaagattga ttgaattgat cagtttttcc tgtatttttg
    34441 taaagaagat aggccaactg tattgggtag cataaagaat caatttcata ttttctttcc
    34501 cagatccaat cgttcatttt tgtatgatct gtttggtggc cagcaccgtt agcttcttcg
    34561 ttgaaggcat tcgcatatgg atcgatattg atataaaaaa attgcttttt gactaacccg
    34621 ctgatcatgt cagctaattc ctcatcttct tttgcaatca ctaaataagg acgaacttgt
    34681 gcagtagagt ctcttaacca catcgctgga atgtctcccg tcaataaaaa ggtggttccg
    34741 tcattttgct ttttgactgt tgtcaacaga gtattggcaa atgctgctgt aaaattcttt
    34801 gcccagtccg catggatatc cccacatttt tgtgtgatat cttccataaa tttctgcacc
    34861 gattttggta tatctttata agccactaat aaaaccttct ttctatttga tatatcttta
    34921 aagattatac ttcatacagc caattatgtc tacgctttca aaaaatatct ttttatttac
    34981 tttttcatcc attatggtat tatttgaata tgatatatca ttaagaggtg tttatatgaa
    35041 tcaaccattg taccagacaa tatttaatga tttgcaaacg gctattttaa ctggtgaatt
    35101 acctgtagac agccaactac caactgaaaa agaactttca cgtacctata aagtaagcag
    35161 aatcacttcc aaacgtgcat tgactgaatt agagcaatta ggacttattt atcgcgttcg
    35221 cgggaaaggc agtttcgtta aatccgcttc tccggaacag attcttgctt ctcctacaaa
    35281 aacacgacgg attctgtttc ttcttccata tgtcgctgat ttgtctgttg gtgattttag
    35341 caagggattg aaccctgtga tgcaagataa acagattgat gtcatgatga cgacgcttga
    35401 ctttctacaa aataaaacag caaaagagct aatcaatgaa tttgacggat tgatttatta
    35461 tgctcttgat acagaacaac atcttgactt actttttgag ctttcattga aagaattccc
    35521 tgtcgtcata ttagataaaa aaatctatga gttacctttt cctaccgttc tttccgataa
    35581 tttccaaggc ggtgcgcttg ctgcctctta tttgatcgaa cagggacatt caaaaatcgc
    35641 ttatttgttt ggtacgcaga ctcatccaca atccgttcga cagcgttacc ttggttacct
    35701 tgaagcattg gacaaagctg gtttgacttt ccacacttca ctggatgaca aaagcgcgac
    35761 attagatgaa ttgctttctt atatcactaa gtatgaagta actgcttttg tttgtgaaaa
    35821 cgatgttgtc gctatccaga ccatgaatct cttgagacag catggttatc atatccctga
    35881 agatttttca attatcggat ttgatgatat ccaagcagca gctcttgttg acccgccact
    35941 gacaaccatc gcacaagatt ttgaacagtt aggtaagctt gcgggagatg ctttggtcac
    36001 ttggttagag acaaaagatc gaccatccga tagtaaattt ccagtttcat tcatcaaaag
    36061 acaatcaaca aaggagaaat aaaatgaata tcggtcaaat agatactaga catgcaacag
    36121 cgaatcagcc aaatttctct aatgggaact gccagcctta tactggcgtt ccttttggga
    36181 tgaattattt tgcaccgcaa actacggatc aaaacggcag ctggtggttt catcctgatg
    36241 accgcgtttt tcaaggctac cgtcttaccc atcaaccaag tccttggatg ggtgatttca
    36301 gtcatatgct gtttacgccg atcaatggaa aactacagga gaacaccctt ttccatgctc
    36361 aaagttctta ccgcccagaa gaaagtatat tttgcccgac tcatctgtcg atctgtcaat
    36421 tacgttatgg catctgttct acattgatac ctagcatgta tggcggaatc ttatctattg
    36481 attacagcaa aaatgacagc ggtttacttt tatcctttcc gggacgccat cagctctttg
    36541 ttatagatcc ttacacagtg gaaggaaaaa tcacacattt tgctggttca gaagatcctg
    36601 atttttcttt ttattttata ttgagatttg aatatccttt aacaattgat gagttagctg
    36661 tttccaacaa aaatagcgac tcgatcccgt tgtattttga acagacaaaa aaacaaacta
    36721 tccgatttgg gacatctttt atcagctcgg aacaagcagc ttataacctg gaactagaac
    36781 aaaaccaaag caaagagact taccttgctc acagtcaaga acaatggcag tcctattttg
    36841 atcgtataca gctcacacac catgatcaac aacaattgcg gacgttttat cataatttat
    36901 atcgggtgtt tttatttcca caaacatttt atgaaattga tcctagtggg caaaaaatcc
    36961 actatgatac gactagtcga tctataaaac ctggtgttct ctatacaaat aatgggtttt
    37021 gggatacgta taaaacggtt tatcctcttt attctttgat tgctgtagaa aaatacgaag
    37081 aaatgctgga aggttttttg aacagctata gagaaagtgg ctatttgcca aaatggctgt
    37141 ctcctgatga acgtggactt atgcctggga cattgatcga tgcggtcatt gcagatgctg
    37201 ctgtaaaaga tatccgacga gacttgatgc ctgaatttct aacggcaatg gaaaaaggtg
    37261 ctacggttca aagcaaacat ccaaattatg gacgccaagg aacagaagat tacttgacat
    37321 atggctatgt tcctaatcac taccacgaat cagtcaatca cacattagac tatgcttata
    37381 gcgatttttg tattagccaa gttgccaagg tactagaaga agaggaaatg acgaagaaat
    37441 atcagcagca atcattgaac tatcaaaata tctttgacta tgagtcagga tttatgcgtt
    37501 caaaagataa agatggcaag ttcagagaac cattttcagc taatcgttgg ggacaagatt
    37561 atgcagaagg aagtgcttgg caaagcagct ttgctgttta tcacgatttt tcaggactga
    37621 tcggaaaata tggtggcagt gagagatata aagaaaaact cattgagctg tgcaatcagc
    37681 ccccaacgtt tgacccagga ggatatggtt ttgagatcca cgaaatgagt gagatggccg
    37741 cgatagaatt tggacaatta gctatttcaa accagccaag ttttcactat ccttacttgt
    37801 tcagttatat aggacaacct gaaatggcac agccactgct taaacagttg atgactcaga
    37861 ccttcgacga ttctccaatg ggttaccctg gagatgagga caatggaagt atggccgctt
    37921 ggtatatctt caatagtctg ggattttatc ctgtcacgcc tggaaccggt gaatatgtta
    37981 tcggtatgcc attaattgac aaggctgttt tgcttttgtc aaatggaaag gaattgacga
    38041 ttgaagcaac gcctaatcgg ccgcagcaac aatttatcca taaaatcaaa cggatggaca
    38101 aagaatatac gaaattatat tttacccatc aagatttgct gaaaggcggc acgatctctt
    38161 accaattagg gatcatccct caccctaaga aatatcaagc aaaagatttg cctttttcct
    38221 tatcaaacta taattaacaa gaggttgtga aaaagctgtc ctgcatcaag taataagaac
    38281 agccaagcaa aaatagatcc tcatattttt cgtttggttg gtcttattac cgcagatgca
    38341 agcttttgaa caccgtttat tgagaggttg tgaaatcagc gttctgacct gagtattaga
    38401 gagacaaatg gcaaaatagt tcctcatatt ttgccatttt ttgagctaat accgagggtc
    38461 agctgattga acaccgttta gtggagaggt cgtgaaaaag ctgtttagtt tcgagtatta
    38521 agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt aataccgaga
    38581 aactggcttt tgaactccgt ttatcgagag gtcatgaaaa agctgccttg cctcaagtaa
    38641 taagaacagc ccaaaattag atttctagac aattttttat aagttatttc attacattgg
    38701 ctttttcttt tatgattctc cactgttttc tcggtactct ttaggggatc tgccgacgat
    38761 tttcttgaat aatttagaga aatacgtggt gttgttgtag ccaatctcac ttgctatctc
    38821 attgatgttt tgattactgt gcaaaagcag ttgtttcgct ttttccatac gtaaatggtt
    38881 caaataagct gaaaaacttt ttttcgtttc ttttttgaac agctgtccta gatacatgac
    38941 atttaaatgc aggttttctg acacttcttt gagtgtaagt ggttcttgat aatgctggtg
    39001 caggattacg attacttttt ccacattgtc actgtacttt ttctgtctct gatgcttttt
    39061 gatgaatgac agaagtagct gctgaagttc ttgcaccgtt tttgcctgat ggattttttc
    39121 gattccttgt aaatattcct catcttccag atcaatcaat tcccgttgaa tctccataaa
    39181 cagtaaaaaa ctggaatatc gtatatcttc cggcatcatg gctgctaact gaaactgttc
    39241 aaaaaaatca gtgatcattt tttgagcgac ttccaattgt ttattttgca gcacgcgtct
    39301 gaaacttgaa aaatcgatgg tttgatcttt tgtttctgct tgtactgcgt aaaaaacatg
    39361 ctcgtcttta tctccataaa attgatgcaa ctgtagattg tctttcgctt gttggaatgt
    39421 ttcagggatt ttgtccaaag aatctgtctc ttccccgata ctgatcagcc attcctgatc
    39481 aggaaagttt tcttgaacaa aacaacagaa gccatcaact tcatctgcct ccaacaacaa
    39541 cgtgatcaat acacggtctc cgtaatttcg ttgatagtaa aagatttgtt gccgttcttg
    39601 caaccatcta ttgataagag tatcatgagt tcttgccagt tgaatcaata ctatcctatg
    39661 ctgcttctca cttatttgtc ctattatttc ttcttcactt atttcatcca attcttcatt
    39721 caaccattga ctgaataaca gttcttgata gatttcttgt tgattttttt gttcattttg
    39781 ctgatctaac cgtgttttga ttttcttgag gctttcaacc aattcgaact tattcacggg
    39841 tttcatcaaa taattcacgg cgcctaattg catacctcct ttcaaataat cgaattcttg
    39901 gtatcctgat aaaatcataa attcaaaatt atgacgttct ttttgagccg cttcaataaa
    39961 ttccagtcca tttatttctg gcattgtcac atccgttact actaaatcaa tctgtcgctg
    40021 ttcaagtaca gctagtcctt ctttggcatt ctctgctgtt gctactattt gaaagcctaa
    40081 actttgccaa tcaattatct tttttaaccc attcaaaatc atgtattcat catctaccaa
    40141 taacacttga tacacgctca tccctccttg aatgtaatag aaacggtcag tccacctgtc
    40201 tcattattct caagcgacat ataataactg ctgccgaagc tggcacgtaa acgttcattc
    40261 acgttttgca gaccgataga accgtgaagt tccactttag gatgagacag tttttcttca
    40321 atctgtttta gccgtttttc agtcatccct tttccattat ctttgatgat gatccttacc
    40381 cgtttgcctt cctgcaagac tttgacactt agcgcattat cgaatcgagt aaaatcaata
    40441 ccgtgtttaa aataattttc aactaatggc tggatcacaa atttaggtac ttcgatcttt
    40501 tccaaatcag gatcgatcat aaaatgatag gctactcgat taggataacg catttgatac
    40561 aaatagacat atttttcgca aaagtccagt tcttctttta aagtgatcgt tttctcttga
    40621 ttcgtattgt ttcgaagcaa agcggaaaac gcatacacaa catctgctaa ttcttcactt
    40681 ccttcgctga ttgcatacat tcggatatat tctaatgtat tatataaaaa gtgtggattg
    40741 atctgtgctt gaagcgcccg catatgtgcg tcttgctgtt tgatttctaa tttgtagatg
    40801 tcttctacaa actgattgat gctatccaac atttcgttga taccgattga aagttccttc
    40861 agttcatatt ccgtgtttgt tatgtcgatc cgcgtctcta aattccctgc tgctgtttga
    40921 tccatggcct gcatgacgat agatacttgc tgtgaatatc gtttgaatgt tttataaaga
    40981 aagataagca gtatattgtc taaaataatg ataccaatga tcagcggggc aagatttttt
    41041 cccgtttcga tttttacact ttttttatct agtacagcta atatacgata atcatcagac
    41101 agttcttggt gttgaacaaa attgtttttt tccagatcgt caatcggcaa ttgtgaattt
    41161 ttcttcagac tctctgtgat gatcatttct tgtctttcaa acgtttcttt agctatttta
    41221 ttatcatgga atgtgtatag tcgatttgtc gttccagaaa tcatgtataa agagagtcca
    41281 ttgaatgacg ttagattttc taaaatcata tctagttctt tcttttgaaa accaataaaa
    41341 atacttcctg actcagctcc tccttgtgtg ataggcaact tgatataaaa ggcgtctagt
    41401 aattttggtg tccccttttt tatttcccca cctttgtttt ctctagaaga ttcataatac
    41461 tccggtagtt gattcaacac gatatatatc gcacttagtt cttcataatt tgcgtagaga
    41521 tctttgattt gatttgggaa agaaactaat tcatcggtac tgttttgctg ttcgtataca
    41581 tacatcagat actgatctat gggttttgtt aagtaggtcg tcacattttc gatcttttcg
    41641 gaattcccta caagctggtt tttgatgatc ctactcaagg ttgttttatc attcagcatt
    41701 ctgcttgtgc tttgaacggc atcacttgtc atgttttctg cctgtttttg attttgttcc
    41761 caagtatgga tactaaaaat aacggtagca gtcatcgtta caccgatcat gataaccgcg
    41821 tatttccgca acagacgatt tagtaaggtt ccttttgaaa atagaatccg aaacttattc
    41881 ataactttcc aactgtttat ctaattcttc acgccaatgt gttgtcatcg taccattcca
    41941 aaccgtcaaa agaccaatga caccaaacag gatcaatcct ttgaattgcc aagtcacgcc
    42001 taaaattata ccgctaccaa taattatttt tagaaaagac gaaaagctgg aaaaaaccga
    42061 aataaaactt agctttagca gattaggaac agagatcgta tattcactat ctaaaatcat
    42121 actgtattga taagtagcaa ataggcagag caaggcaaaa agtaaaataa aatcaatagc
    42181 taaaaaaatg agccctttga tttgaacaga taaatataaa ttgtaactca ataaagcagt
    42241 accagataaa aataaactaa atagcagatt gcctcttaaa aaatttcttt tatatatatt
    42301 ccagcctctt tttattgtga tttctttata ctcgaatccg tacaaataaa atagttcatt
    42361 gactgttttc catgccgcac ctaatccaaa gatgatcccg ccggcaaatg tatatatcca
    42421 gaaatacaag gttagtttta taatgaccca gatccggata aataactctt ccaaaacctt
    42481 ccctaacata tcttctcctc caaacattaa gtttatactt tctcattata caaaaaaagg
    42541 ttgggcaatc agcaccttga cctgattatt agagaaacaa acggaaaaat agttcctcat
    42601 attttgtcgt tttgtgagcg aataccgagg gttagctgat tgaacaccgt ttaccaagag
    42661 tttgtgaaat agccgcttag tttcgagtag taagaaaaat tttgtgaata tggctttcca
    42721 catttttaca tatatttagc ttaataccga gaaactggct tttgaacacc gtttattcgg
    42781 aacaagaggt tgtgacacgt cttttgtcac aacccctttg tctttattca ttattattct
    42841 tgtcggaact catcatattg tttttgcatt tctttcaata ccttatcata gccagccttg
    42901 tccaaagcgt ctttcaattt cggtaatgtt tcatctggat caacagtccc tgtattcaat
    42961 ccatctaaat attgattcat gacattagaa atattactta attctgtttt gacactgtca
    43021 gtgttgaagc tgaatcctaa aatcggtgaa gtttctgcat cagcaatcga ttgatcacgt
    43081 ttggcaatca tgtcatccgt aatagactct tgtgtatata gaatcttgtt gtttcctgta
    43141 ttccaagcag acatatgtgt atttggctga taaccatcta gcaatttgat tttatcatct
    43201 gaaccatcga ctttttccca tgcttctccc tcgatccccc aaacaagccc attcaataat
    43261 tctggatcac tgttaagcag ccctagaagt tctactgatt tttctttatt ttttgaagta
    43321 tttgatacga caaaatttgc catttgtgct tgagctgtag attttagtgg aacagtaatt
    43381 gcttttgaga caagttcttg ttttgctgca tttgtcaaaa ttgtatcgcc ataatcatac
    43441 ggtccttgtg tttctccacg cattagccaa gtatttcctt ctagtggata atccttattg
    43501 ctcgttgcag cgtcacttgg aatgagtcct tgtttgtacc agctatgcat tgtacgtaac
    43561 agtccgatat acgtttcatt atcgtattgg ttgatgattt tcgaatcatc tccatccaga
    43621 tcaatagcaa atggtaacgt attccctaaa ggataatcaa agttcccttg gactttgaag
    43681 ccttgtccaa tggcaaaagc tgccgtgttt ggttcttttt catggaattc cttcaagact
    43741 ttttctgcat cagcgtaaga ttggattcca tcaattgaaa gatcgtattt atctagtaaa
    43801 gctttgttaa aagtaagtac ttgttgagca aagacatttc cattgactgg gaaagcatat
    43861 aattttccat tgaccagatt tcctttaata tatgcttcat ctaaatcatt ataagctttt
    43921 tcagcgtact taggcgctaa ttcagtcaaa tcagcaaagg ctcctttttg ggcattggaa
    43981 acatagttgt tcgcaaaggc gatatcataa ttttccccgg aagaaatgat gacgttcatt
    44041 ttcttttcgt aatcgcccca gccaatgtaa ttgatattga cttttacacc gatctctttt
    44101 tcgattcgtt tgtttgctac ttccatcaat tgatcgaaat tatctggttt atctccgatt
    44161 tgatacatct gcaaagtgtc cgaattgcta gcttctgcct ggtctttaga accagaacct
    44221 ccgcatgctg ctaatgttcc caacaacaac gctgaaacac ctgtaaaggc tagtttcttc
    44281 cataacttca tttttcttcc tcctaatttt atgatttata tttttgtttc tttattcttt
    44341 tactccgccg attgttagac ctttgacaaa atgcttctgg aaaaatggat agctgatcgc
    44401 aataggcaac gtcgagatga ctacgatcgc cattctggca gattctcctg gaactgaggc
    44461 taatccgcct gtcaattggc ttcctgctcc tgaattttgt gtcagatatt gaatattact
    44521 ttggattttc atcaaaagat actgtagtgg aactagctta tcatcttgga tgtatagtaa
    44581 agcattgaac cagtcattcc agtaacccaa tgcggcaaat aaactgattg tcgcaatacc
    44641 tggaacagct aacggtaaaa cgatctgaac aaatattcgc agttcgctag ctccatcgat
    44701 ccgtgccgat tcaataatcg aatcagagac cgaacgttta aagaatgtgc gcatcacaat
    44761 aatattgaag ggactgacag ccattggcaa gatcaatgcc caaatcgtat cttttaactg
    44821 aagaagattt gtcataacaa gataattggc caccatccct ggtgagaaca acatcgtaat
    44881 caaacaaaaa acggtaaaaa aacgacggaa tggaaagttt gaccgtgaaa tcgcataagc
    44941 atataaagaa gtagccgtgc tattgatcag tgtacctaaa acagtaacaa gtacagtgac
    45001 gaaaagcgct tggaaaattt tatcctgcat ctggtcgaac aagtacgtat aaccaaatat
    45061 actgaacttt tctggccaaa aactgtaccc gtgagtcgac aatatggact catcggtaaa
    45121 agagatggcg ataacaaata taaatggcaa aatacaggaa atagcaaaga gtgcaatcat
    45181 tatattagag aaaaaattag ccgtcttatt gaaagagcgg acttggactc tctccacttt
    45241 cttttttttc atttttttcc tccttagaat aatgcagaat cgctatcaaa acgtcgggcg
    45301 atcgtatttg tgatcatcaa caagacaaag ccgacaactg attggtaaag tcctgcagca
    45361 gccgtcatac caaaatctcc tgtggatgtc aggccattgt agatatacgt atccaaaact
    45421 tgagtcacat catataatgc accggaattc ctaggaatgt tataaaacag gccaaaatca
    45481 gctcggaaaa tgtttcctac ggctaaaatc gttaaaatcg tcattagtgg aataagttgt
    45541 ggtatcgtca catgtttgat ctgctgccat ttgctagctc catcaaccat agcagcttca
    45601 taataagtcg gatcgattcc cataacagat gcaaagtaaa taatgctgtt ataaccaatc
    45661 cctttccatg taccaatgaa caccaagata aacggccaat atttcggttc attataccaa
    45721 ttgacagctt ctcctccatg tccagtgatc cactgattga atattccttt gtcaggactt
    45781 aaaaaagcgt aaacaaagta actgataatg acccaagata gaaaataagg tagtaaggac
    45841 atcgtttgat aaactttaac gagtcgttta tttctcaact cgctcataat gattgcaaat
    45901 acgactgaga tcagcagatt caaaaggata aatgctacat tatataaaat tgtattttta
    45961 gtgatgagaa atgcttgatc cgaagaaaat aaaaatttga aattttccca acccacccaa
    46021 ggactttccc tcaagctagc caagaaccca tctgctgaaa tatgaaaatt tttgaatgct
    46081 acgacattcg ccattacagg tatgtaaaag aaaaaaatca tccaaagcat acccggtaca
    46141 gccatcagga caagtgcttt ataacgccat agattcataa aaaaatgatt gaaccccttt
    46201 cgttttttca tctaaaaacc cctttcgatt cattcgcaac gtaattatag cgctttcaat
    46261 aatgagaata aataaacaaa gtatagggaa aatgaaacaa agtataggag aggttgtgaa
    46321 atcagcgttt tggcctgagt attagagaaa aaaacgggaa aatagttcct catattttgc
    46381 cgttttttga gctaataccg agggtcagct gattgaacac cgtttatcag aggttgtgaa
    46441 ttcagcgttt tggcctgagt attagggaga tcgtgtcctt actatctaac tctgagtcac
    46501 aaggaacaat ggggactgta acaaaagtct tgtcacagtc ccttattccg aacaaacggt
    46561 gttccagaag tagcttctac ggaaataagc cgaaatttca caaaaaagcc atccgaaagt
    46621 cagacggaca agtctgcttt tcagatggcg ctacaactac tctgcttatt cttttagcta
    46681 tccattcaat tatttttctc tcacttgttc tagctcttct ttggtcatgg gtcgatattg
    46741 ccctttattc aaatctttag gcagccaaaa ttttcccata cggattcttt ttaaatataa
    46801 gacttctttt cctactgctt tgaccattcg cttaacttgg tgaaactttc cttcatgcaa
    46861 aatcagacgg ataattgaag tttctgtctc ttgatctact tgatcaattt ggagttttgc
    46921 cggcaatgtc tgctcatcgc catcaataat cagtccttct gcaaataaac ggatatcttc
    46981 ttctgtcatt ataccgctaa cctcagctag atattctttc tccacatgtt ttttaggtga
    47041 tagtaactga tgggcaaaca ccccgtcatt cgttaaaatc aagaggccct ctgtgtcttt
    47101 atctaatcgg ccgactggaa aaagatcttc ccgataatcc tggtcttcca ataaatcgat
    47161 tacggtttga tcatgattat cctgagtagc agaaatgact cctgcaggtt tatgcaaaat
    47221 gtagtaaaaa tctttttgat acgtaatgat ttctcctaaa taagtaaccg catcttcgaa
    47281 ttcatttact tgcgctttat cgcttttcac cacttgtcca ttgacttcga tcgttccttt
    47341 tttgattaat cctttgactt ctttgcggct gccaagtccc atatctgcta aaaacttatc
    47401 taaacgcatg cgtttccccc atttcaaaag agcatcaatc tagctcttct gattcactta
    47461 ttttattttt aatttatgtc gtaaacgcgc tattcctggt cctagtagtc tttctgccaa
    47521 gcggattttc aaagctaaat aaccatacac taaagcccct atacctgcta ctacaagaat
    47581 caacaataga gactggaatt tgctatcttc gtttaaaaaa gcaccaaaaa tcatctttgt
    47641 gatccacgaa gccaaaagca taatcaaggt aagaataaag atcagcaatg tcctgcgcca
    47701 gacaaaactt ggtttaaagc gggctacttc atggattctc tttaagatca aggtacaaga
    47761 cacagcaaat ccgatcattg tcgccaataa agggccgtat acttcaaata aacgaatggc
    47821 tgggtattga agaaggagtt tcactagaaa cccaataacc aaataattaa ttgccgcctt
    47881 gttttcaaac attccttgca gcatgttgga aactagcata tatagtccta ggaagagtcc
    47941 tacaaaactt gcttggatca gtacattact tcctagagaa tccggtgtat aaaataatgt
    48001 atttagcgga taggcaagta acataacacc aaatgtcgcc ggaaacataa aaaatgaaaa
    48061 caactgtaaa tttccgcttg tcaattttgc cagacctact cgatctttca acgtaacagc
    48121 ttctgtaatc aacggtaacc cagtagacgc gatcgatgtt gccaatgcaa taactaccat
    48181 cgtcaactta tctggattgg cactaaaaat cgaaaaaaga tccagtaact gtgcattgga
    48241 atactcggtg gatgcagaca tgatcttcat aaaagtgaac tgatcgacta atttgaagat
    48301 cgttacccct gaacccacaa taataaacgg aatcgcttct ttaacggtat ctaacaacaa
    48361 ttccttcgtt gcaatattta ttttgttttc gctgtagtga ataaacgccg atgtatacgc
    48421 ttgatgtttc ttcaaaaaat acaaaaggac aagaatactt gctagcatac cgataaatgc
    48481 cgcgaaagtt gattgcgtga ccgccgtcac atagtcacca tccatgactt tcatgatgat
    48541 gaatgtagcc aatagcatat aaaaaatacg tgccacctgt tctacaatct gcgacagcgc
    48601 ataaggcatc atttcttgat tgccttgcaa ataccctcgt atcacactca tacaaggaaa
    48661 aaccaataca gcgatactca aagaacgcat cgttggaatc agttcttctc cgccaccaga
    48721 agcatgagct agccaaggag aggcgatgta catgaacaaa gcaaacaaca ggcctaaccc
    48781 gcccatcatc tgtaatgcac ggataaataa tcgtcgtgaa gtgccatatt cattcaatga
    48841 attatagcgt gcagtttgtt ttgcaatcgc agcaggtatt cctgctgtag agatcagtag
    48901 aaacaatgca tatatattgt atcccatatt gaataatcca ttggcagcct tagcattttc
    48961 gcccatccaa gcataccagg gaatgatata gacagcgcct agcaatcttg aaatgatatt
    49021 actggcggtc atccaagcag agcctcgtgc cattttttct tgcggtgtta attgatctaa
    49081 tgctggtttt ctttgattat ccatcggtca gattccaact ttctacaaaa cattactatc
    49141 attttaattg gtaaaacaga aactggcaat cgattttata taacttttat aagaacaatt
    49201 gtttttttga attcggtttc gtttttttgg taaagtaaag aaatatacag ccttttatgg
    49261 ctaacattta taggagatat caatgacaca agaaataaaa aaactacccg ttatattttt
    49321 aggtcttttc tttatcacag ttggattgaa ttggttcttg ctccctcacg atatagcttc
    49381 cgccggagtg ggcgccatcg gacatttagt tgaaacggcc ttttctatcc aacgctcagt
    49441 tactgtttgg agcatcaatc ttatgatgct gcttctagcc gctgtcctat taggaaaatc
    49501 agtatttttt aagactgtag cgggaagtct gctttttcct gttgttttag agattgttcc
    49561 aaaagtggaa atgatctcct cacatttctt ttcactgatt tcaggaagtc ttttgttcag
    49621 tcttggggtt tacacattgt acacagtcgg ggcctcaaat ggcggtataa cgataccgcc
    49681 gattattttc cacaaatttt ttcggcttcc aatgccgcaa ggacttttac ttaccaatag
    49741 tcttatcgtc tttttgaatt attatgtttt tggattgttg gagacactat tcgttctcct
    49801 atctattaca gtcagttcat tgtttatgaa aatactcatc cgcttgtccc ctgtgtcaag
    49861 aaaatttgaa taaagctgga ttttgtcata gcggaaacaa acgatatatt aaaaagagtg
    49921 tgctataata aaccaagaaa ttgaataaag cgagggatca atcaatgagc ttctctttag
    49981 aagacatccg tcatttacta ttaaaagaaa atctattaaa agaatttgtt tcaacaagag
    50041 gctggcatct tgatgtacca gaaaaagctg aatttcttca gttaagttat gattcaagaa
    50101 aagctgatgc ttctactttg tttttctgta aaggaatgaa tttcaaagaa gaatatttgg
    50161 attcagcaat cgaacagggt attcaatatt atgtatcaga acaaccatac gaaaattcag
    50221 cagtaggaat catcgtaacg gatatccgta aagcaatggc gcttcttgct atggcttttt
    50281 acgactatcc gcaaaataaa ttgaaagtga tcggattcac tggaacaaaa ggtaaaacaa
    50341 ctgctgcgta ctttacaaaa gcaatcttgg atcacacaac aaataaaaaa acagcattgc
    50401 tttctactat gaatacaacg ttagatggca aaacatattt caaatcccat ttgacaacgc
    50461 ctgagtcatt agatttatat cgtatgatgt cagaagcagt tgaaaatggt atgactcatc
    50521 tagtgatgga agtttcttca caagcttaca aaacacaacg cgtatacggc ttgactttag
    50581 atgtcggaat cttcttgaat atctcgcctg accatatcag tccgatcgaa catccgacat
    50641 ttgatgacta tttctattgc aagcgacaat tgatcttgaa ttcaaaaaca gttgttttaa
    50701 atcatgaaag tgactatttt gatttactta gggaaacagc cgatctattc cagattccaa
    50761 cgatcactta tggtcgttcg gaagaaagca attatcaagt gatccgttca gataaaggta
    50821 ctcacggttt tactttatct tctcatgaag atcgcttagc tatctgcgat actgcttatg
    50881 atattttgct ggctggagga ttcaaccaag aaaatgcagc cagtgcgatc attgccgcag
    50941 cactaacagg tgcctccagt caagatgcac aagacggatt aaaagaagct cgtgtaccag
    51001 gccgaatgga tcaattgatc caatccaatg gagcgcatgt ctatgtggac tatgcacata
    51061 attatctaag cttgaaaaca ttgctgaaat ttgccaaaaa tgaacatcca gatggccgtg
    51121 taattgttgt attaggcagc ccaggaaaca aagcaatctc ccgtcgacat gattttggaa
    51181 aagtgctatc agaaacagct gacatcgcct ttttgacggc agatgaccca gcatttgaag
    51241 atccgaaaaa aatcgcggaa gaaatcaatg aagcaattac taatccagat ttgatcgttc
    51301 attatgaaat ggatcgtcct gaagcgattc gacgcgcttt agcagaaagc gctccgcaag
    51361 attcagtcgt tattgcggga aaaggcgtgg atccctatca aaaaatcaat ggtgtagatg
    51421 aaccttatga aggggattat gcaatcgtga agcggttgat tgaagaataa tggtatttat
    51481 atactagtgg tttcccattt ctaagaaaaa aagggactgt gattttttgt cacagttcct
    51541 tttttcttag aaatcatttt aacttttcac tagctaattc tgctgccttt tctctttctt
    51601 catttaataa tttattatca tagaatttga taaatgggaa ccagataatg aatgcgatga
    51661 ttgcacaaat gactgctaac acagcagctt tccaatcacc accactacca acaaatgctc
    51721 cgatcccgac tggtgaaggc cacggcatct gtgcaagcat cggtttgacg atttccagcc
    51781 gaatggcaaa ataagcgagt gtagcagaag ccatcggagc taagaaaaac ggaaccgcta
    51841 aatagggatt gtagacgatt ggcatcccga aaatgattgg ttcattgata ttgaataatc
    51901 ctggtacaag tgaggctttt cctaacactt tcagctgatc agatttcgcg aggaatgcaa
    51961 taaagatgat caatcctaaa gtcgcacctg atccaccaac ggttacataa gaattgttga
    52021 attctcctgc caacggataa tttgcgcctt gtgcgttagc tgccatatta gctaatacga
    52081 tcggggtcaa gaaagaagtg atgatattcg ctccatggat ccctacgatc cacaatgcat
    52141 ggatcaagaa ataaatcacc atgacaccaa gccatgtatt cgtcaactgc gtaacaaacc
    52201 caaatgggat agcaatcatt ttgaagatat ccgttcctag tgcaactaat aatccattga
    52261 tgatcatcac cacaaacgca atgacaaatg ttgggatcag agcagtgaaa gaacgagaaa
    52321 ctcctggcgg aacaacatct ggcattttta tgacccaatt tctcttgaca cacatacaat
    52381 acaattcgac cgcaataatc gacataataa tagctgtaaa gattcctgaa gttcctaaac
    52441 gttcgacaaa agagcccatc ctaataccat ttactacagt ttctgtctca ttgatagaag
    52501 caatcaagct gattttacca tcagtcatga tcaattcagg gatacacata aagaatgcaa
    52561 aaacagatag taatgccccc gtgagcggat taacatttaa tttttgttca tctcgttcaa
    52621 ttgacgttaa ttcataagca aacacaatgg caaagtaaat agataaaatc cccatcgtca
    52681 ttgtattgac gatcatgtac aagtcactaa ctttgaaaaa agttgcatta aaaaatcctt
    52741 ctaaaaatgg gaacggtaat ggcaaaatat ttaaaaccaa aaacatcgag cctacaatcg
    52801 tgaaaggaat agaagccatc cccgctgcca tgacagcccg aacgatttta agttgcgcaa
    52861 ttttccccat aggtcccatc aggtatttct gcaaaaaatc aaacattagt tattctcctt
    52921 tcctaaatat aacgcttttt catattgttt aatacggata tactgttggt cttggttgag
    52981 tgaaattttc tttaaacgat actgtataaa tgcactggca aaaataccgc ttacaacaag
    53041 aatgaaaaca aataactgtc ctccattgtt atttgccata aaaggataca agaatgtaaa
    53101 tcctgaggta aacaacactg gaatcactaa taaattaaag acactctgga acaaaaaata
    53161 ccactttgtc catggaacgt tcgtttggtg ttttcgataa agctttactt gttctcctac
    53221 tgcaggcaaa atagctaaaa tcagaatcat cggtaaacta cttcctaaac ttttagctga
    53281 tagaagcaga atcatccatt gcatgttcat gaaaaagaaa aaggcagtca ggtaacgtac
    53341 gattagataa cgattgaaat acatgttttt caaactaagt tgcttttttt ctaattcgat
    53401 tttccttttt actgtatcct gcacaatgat ccttcctttc tatttatttt tttgatacaa
    53461 ataattcatt tcctgtgcaa cttccaataa tgtcatcgtc gtcatcagat gatcttgtgc
    53521 atggaccatg ataatttcca tttctatttt tgtcccgctg gcatattctt tcagaagttg
    53581 agtttgtgac tgatgcgcat gaagaatctc ttcatttgct tgtcgcaact tctcttctac
    53641 ttcttcaaac atctctttac gcatcgagcg aaatgcttca tgtatcagtg tccgtgcatt
    53701 tccgctatgg aaaatgatat caaaagaagt cacttgtagt gtttcactgt ttattttttc
    53761 agccatctgt tttcctcctt attcgatcag tttgataaat ttactgctga aattctcaaa
    53821 aataggttca gctaagattt cctgctgtac ttttagctgg tcaactaacc taacgattgc
    53881 tttagtcacg accgtaattc cttcattttc gacataagaa ggcgaaacta aaaaaacaaa
    53941 atgaatttct tgatattgat cccactgcat cccagaagga atcaatgcaa cggctacctt
    54001 tgtttcgatt ccaactggaa cagcgggatg tgggactgcc actgtatcac tgaatatgat
    54061 ttgtcccata ttttcacgtt gctggatctg ctttcgcatt tctcttatat atccttcttc
    54121 ttcatttatg ctcagctttt gcagcaaatc atgaagaatt tcttcctttt tcggttcgta
    54181 cgtataagtc ttgaaaaatt tttcagacaa ctgttcggtg taaatctgct ttttttgctg
    54241 tagagaaaat gctggtatcg gtctatctga ggaatgatag ggtatagatt ctccaactac
    54301 ctttcgtatt ttacgaacat catcttcatt caaaaaaata ctgacatgta aaactggaat
    54361 tttgaaaaac attgtcgaaa gatcaataga agaaataatc aggtcgattc cttttaaagt
    54421 tgtttcattg atctcataat agccttttac acttaccact gtaatatttt taccgaattc
    54481 actgactacg cgattcttta atagttgggc actgccgtaa cccgttgcac agataatcaa
    54541 tgcatgtagt ttatgcgcag ctctttcttt ttccaatgcc gccatgagat gcaaagccaa
    54601 ataggcccat tcatcttcat tgatggtata gtttttcaaa gccggcatgt tgctgaaatg
    54661 cctttttact gcgtcaaaga ctttgggatt ttctttcttt atctcctcag tcaatgggtt
    54721 ctcaatgcga atacctcggc tcaatcgaat catcatcggt gacaaatgat cgagtagtcc
    54781 attgaaaagc tggagatcct ctactaatga aaaatgaaat tcctgagaca atcttttcag
    54841 aactttcgct aattcctttg atagttcctg atcattttga ttttctttgt gattcgactt
    54901 agccatgaga tgtaatgtca aataagcaat ttcttcttcg ggaaacgata cacctgcgat
    54961 tacctcaata cgttgaacga tccgctgcgc cacttgatac tctactcgct tagaaacttc
    55021 ttgttcgatg cctaattcct tgatttcaaa cccttctcgg agccgtttga tccccaaagc
    55081 caagtgtaaa gtcagattct gtatgataaa atccgaaact tttaacttag cttctcttgt
    55141 ttcatcgaga atgataatga ctagctcttc aaaactgatg tctgggaaaa aacggctttg
    55201 ccctaaatat tctttcaatg aatttccata atggtttcca aaaaaagtat ccatgataaa
    55261 atgccgtcga tttctttctt ccccttcgat ccatgtccct tgaccatgct tagaaacgat
    55321 ttttagtgaa tatggaatca atcgctcctt gatttcttgc atgtcctttg tcaaactgga
    55381 acgactgata taaagctctt ctgccaaatc atccataaaa agaatggcat cttccaaaag
    55441 caattggttc aatatatact tttgtcgatc ttcggactca taaaatcctt gcggttctct
    55501 agcatatcca ggtactattt cttgccgact caaaaacaag tcaaatgtca gtctttgtaa
    55561 aatatgcagt tggtagccat aacctggttt agcaatgatc ttacctccgt ttttttctac
    55621 taactctttg atccgctgca agtaattacg gattgtccga tcagataata acaattcaga
    55681 agctaaacgt tgactggtga catactcctt cttgtgatca atcaaatatc tcaacagcaa
    55741 tttttccttc tgtttcatta tcatccactc ctattccatc atatgttctc ttcgatcacc
    55801 tctgctaact tctggatgcc tgtagggatt gggacatacg cttggaaagg gatactaaca
    55861 acaggctttc caacagattc tcctaatttt ttgaatttat ccaaaaacat cgtcgtttgc
    55921 gggctgatca gaaataaatc aaaatcacta ttcttaatca tattatcgcc ttctgtagct
    55981 gaaactgcat ctacgagtac ctcttctctt ttttgcttaa agaaatccgt tgtctttgag
    56041 gccatcatcg aggatgacat tcctgccgca caaataatca atgctttttt cataaatcaa
    56101 tcactccttt tatttttata aaccaagcat atcttgtaag cgttttctat tcaaaatata
    56161 tatttccgta gcattgcgga aaaaatgtgt atcatatctt gaataataat ttctggcacc
    56221 gtgatatact agtgatagaa ttttttcagg aggggattta gtgagtatca cagtgaatat
    56281 tttgtataca agtacaaatg gacaagccag aaaatttgca gaagaaatgg tcgaaagaaa
    56341 cattgtcaaa gcgattcgtg cagaagaagg aaacgagaaa tatgactatt actttccact
    56401 agaagatcca gaatctcttt tactgatcga ccgttggaaa gatgaagaag caatcgaaaa
    56461 gcatcacaaa tccgaaatga tggctcaaat aaacgaactc cgtaaaaagt ataaattaaa
    56521 aatgaaagtc gaacgtttca ctgatttacc gtaaaaagat acacatggaa tgatagcaat
    56581 ttatcatcca tgtgtatctt cactttttcc ttatttttta ttcgatctca tctaatgatt
    56641 caaaccgtgt ttttctctta tttttcggat aagtatttca gtcaccccaa tatacatgat
    56701 tcctaaacat accccaagga tattctcaaa aatgcggatc gttactgctc ctggaacccc
    56761 gaataaactg gcagtgattg ccaaagcacc aaaagaatta aaaactgttt tatatctata
    56821 ggtagaacaa acacctaaag ctagaccacc taatataccg acccagttaa aaggaataaa
    56881 ttggctgata ccaataaata aaacacatcc aatcaatgag ccaatgattc ggtctactgc
    56941 tcgttctttg aaaccaatcg acatcaatcc atagctagat acaatagaag aaaatgcaaa
    57001 ccctgcccac atcaatcgct gaaaagggac atactctcct gcaacgatca aaagactaat
    57061 gccaaatgca tagatgagca tccaaatttt ttcctgcgaa tacatgcctt tcaaaaaaat
    57121 ctctttaaac aagcttttgt ctttgttctt ttctcgatgt tttctgtaaa ggagaactga
    57181 aaaccaacaa aaaaacaaaa ataataagct acttgtttgt gtaaagaaat tcttgttcaa
    57241 cgaatcttta ggtaacgaat aaacaataaa gagatatgaa aagccgtaca aagaagcatt
    57301 ccccattttg ggatcactag cggttgccat taatatgctg cttagtaaaa taaaatgaat
    57361 caacatgccc ataactgacc atttgatcag ctggacatac ggtgctacta atagtgagaa
    57421 catgacgatt gctaaaccta tgatcgaatg ggaaatctta taaccaaagt caacaaaacg
    57481 aatactcatc aacatacaga ataaaaccac agcgtatggt ttatgcttcg attcaaataa
    57541 tgttgtaatt ataatgataa aacaaatggc aaacaatacg atgaataatg atcgtaaaaa
    57601 caatgaacaa atatacatgc acttttcttt tttagaagct gcttctcgta ttttttgttt
    57661 taaaataaac ggatctagtt gtagagactg ataaaaatcc acctgttgtc gtcctttctt
    57721 tttactttta ttttttacag tcatctatga caggttgtaa atactatgga acatcatatg
    57781 gcgttgtcat tttcttgtca actatttttc ctattcatgt ttttataaaa aaacatatcg
    57841 ttttcttgta tgattaaatc accacaaaat aaacaactga gagaacgata tgctttatga
    57901 atggttctat caaaaaaatt ctgagatcaa tagacaaaaa tttgatgatt acagaggtct
    57961 cttacgaaac cttacagaag aaaaaaacat tgatcgttgc tgcagttctc ttccctgctc
    58021 catgtgtctg tagaaattgt ggttctgctg tggtagatgg aaataggaaa ttaatggtgg
    58081 tgaaaaacaa gaagaaggaa accattgtcc actttgaata atataatcat atgccaatgg
    58141 ttatacgcct aaaaaagcag cgctatacct gtaaaaactg tcgaagccat tggaacgctc
    58201 aaagttattt tatccgtcca aggcactcga tttcaaatca tgtcagacat aaaatcactt
    58261 ctttactgac tgaaaaagtg tcgttatttt ttatttcgaa aagttgttag gtatctttga
    58321 caaccgttat tcgcacattg aaagagttga gaagctattt accaaagtct tccaaaagaa
    58381 ttctgtcttg agtgttgagg gttgtgaatt tcgttctcac gtttccgttg aggataaact
    58441 gagttttatt tccgcggatg gcgagacaga aaaattaatc gagaaaattt acctacacaa
    58501 aaaattcctc gattaacaaa ctatttcctt agctgcacta atcctaaaga aatagaattc
    58561 ttagttacag acatgaacgc cacctacttt cagctcacca aacatgttct accaaatgcg
    58621 aaattggtga ttgatcgatt acttggtttt tccacgcctt tcaaggaggc gtaaccttta
    58681 ttcacgagtt ggttgaagct ttccgagaca aaagacccga cttgtttttc tccttattga
    58741 caaagagcca ttaaaactag agaaaaaatt acaactgatc gctgattatt tgtagaaaga
    58801 aaatcaaagt attacccaat aaacttgagt cattaggcaa aaaacactgc gaaactcaag
    58861 aatgattttc gcagtgtttt tgt
//
LOCUS       DACDEG010000010        53255 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.010, whole genome shotgun sequence.
ACCESSION   DACDEG010000010 DACDEG010000000
VERSION     DACDEG010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.010"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            39..203
                     /locus_tag="IV142_000664"
                     /pseudo
     CDS             39..203
                     /locus_tag="IV142_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
     gene            451..669
                     /locus_tag="IV142_000665"
                     /pseudo
     CDS             451..669
                     /locus_tag="IV142_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296656.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(798..2006)
                     /locus_tag="IV142_000666"
     CDS             complement(798..2006)
                     /locus_tag="IV142_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEnfa110 family transposase"
                     /protein_id="HAP8484343.1"
                     /translation="MNCVGIDVSKGKSMIAVMRPFGEVVVSPFEVRHTANELSELAGL
                     LKSLDGETRVVMESTGNYHAPVAWLLHDAGLYVSVVNAMLVHDYGNNSLRRAKTDKKD
                     AVKLANYGLDHWLTLPRYIPEEDTRLMLKICYRQYQQYSKVQTMLKNNLISLLDTTFP
                     DANRLFTSPPRADGSEKWVDFVATFWHCECVCGRSEKAFTTQYQKWCRKHGYNFSEDK
                     ALDIYASACRHFGVIPKTDTAKLLVEQAISQLQTTSSALAALKQEMQSLAASLPEYPV
                     VMGMFGVGPTLGPQLLAEIGDVRRFHSKKALVAFAGIDAPPYQSGQIDVRSRSISKRG
                     SASLRRTLFLVMSVILQCAPIDEPVYQFMDKKRSEGKPYRVYMMASANKFLRIYYASV
                     KAYLESLEHD"
     gene            2296..3261
                     /locus_tag="IV142_000667"
     CDS             2296..3261
                     /locus_tag="IV142_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8484344.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            3283..3495
                     /locus_tag="IV142_000668"
     CDS             3283..3495
                     /locus_tag="IV142_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP8484345.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            3570..4046
                     /locus_tag="IV142_000669"
     CDS             3570..4046
                     /locus_tag="IV142_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HAP8484346.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            4043..4543
                     /locus_tag="IV142_000670"
     CDS             4043..4543
                     /locus_tag="IV142_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
                     /protein_id="HAP8484347.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPS
                     APVRAV"
     gene            5103..6920
                     /locus_tag="IV142_000671"
     CDS             5103..6920
                     /locus_tag="IV142_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8484348.1"
                     /translation="MRNPIHVLKSLEEKASVSNYKYERLYRNLYNPEFYLLAYANIAK
                     SQGSMTQGVDGQTLDNMSLPRINRIIESIRNRTYQPKPAKRKYIPKKNGKLRPLGITS
                     TDDKLVQEVVRMILEAIYEPTFSNNSHGFRPKRSCHTALTQVKKNFTGVTWIVEGDIK
                     ACFDNFDHHVLVELLRKRISDEAFIGLIWKFLKAGYMEQWQYNCTYSGVPQGSGISPI
                     CANIYLSELDNYMQEYKEKYDCEPERRRTTREYERASRRYRKARKALMGAEKSTPELV
                     KEFKDSRRKKMNQHYYNPFEEGFKKIQYNRYADDFVIGVIGSKKDAEKIKEDVKIFLQ
                     EKLHLEMSEEKTKVTHSSKPVRYLGYDFKVIHSKNMKRCKNGDMKRVWYGKVFLYMPK
                     EKWIKKAMERGAIQVKRNNDTGKEMWRPMPRKDLMNRSDAEIVSTFNSEIRGLYNFYR
                     IAENVGALHKYYYMVRYSMLKTLAGKHRTNVSVIKKRHMVNGVLRIPYDTTKGRKYCE
                     FYHDGFRKHSDGYDNVADVMPSYRKYDSRHTIVNRIKAGVCEICGEHADYLCMHHVRT
                     LKSLKGRDIFEQKMLKMRRKSLALCPDCFELLHETKESR"
     gene            7029..8354
                     /locus_tag="IV142_000672"
     CDS             7029..8354
                     /locus_tag="IV142_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006771541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP8484349.1"
                     /translation="MQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTIN
                     FSKSMKYNPFVYIHSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIW
                     YEAPAEEMNFSTLLEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLL
                     SAGKTRSSILISCGARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFN
                     FILAMLQSQLINLLCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREI
                     SASIILQSQSQLKAIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTS
                     ENRGSQTSHGLNYQKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYK
                     YLADADKKNTFDVDRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            8431..8592
                     /locus_tag="IV142_000673"
     CDS             8431..8592
                     /locus_tag="IV142_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003509345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484350.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKHLMSSM"
     gene            8633..9013
                     /locus_tag="IV142_000674"
     CDS             8633..9013
                     /locus_tag="IV142_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HAP8484351.1"
                     /translation="MTISCKAKGDTMKIEYTKVGDYYLPNLYYPEETRPIGLWGMLRK
                     EYLKEHKSGTYTYLLLTARLDSYLADLNEQAQERFELIETQMRSAEGVSEELKRQNPM
                     EWLCHCNNIRNRAAEIIKQELIYV"
     gene            9028..9309
                     /locus_tag="IV142_000675"
     CDS             9028..9309
                     /locus_tag="IV142_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484352.1"
                     /translation="MRRELIESYYEEYQRELERFLESDPEMFTLEAEIHHYIEILEPV
                     LRDGGQEMWQTLDRIISAKNAMEGIVAKEMYIRGFLDYERLVCEKDSKI"
     gene            9568..10350
                     /locus_tag="IV142_000676"
     CDS             9568..10350
                     /locus_tag="IV142_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAP8484353.1"
                     /translation="MKVKNADEFYKILERKAIYPVFQPIVNLQTGDVAGYEALSRIDR
                     HDTTLMISDLFVIAEQVGCVWKLEKLCRNKALKAAANKPEHAKLFLNVDGNIIQDKSF
                     IQGFTNRKAAKAGVPSCDIVFEITERSDIENYQILQQIMKHYADQGYEIALDDVGAGY
                     SGLNRVVNTSPNYLKVDIELVRDIQKHKKKEIMMEFLLHYCNETGAILIAEGIETEAE
                     LECLCRLGVHYGQGYFLGRPDRTFKNVRNEAMLVLRRLNNKS"
     gene            10523..12241
                     /locus_tag="IV142_000677"
     CDS             10523..12241
                     /locus_tag="IV142_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HAP8484354.1"
                     /translation="MNENVKVVFGLIGGLALFLYGMNSMSDALQKAAGERMKKILGFL
                     TRNPIMGALAGALVTAVLQSSSATTVMVIGFVSAGLMSLPQAISVIFGANIGTTMTAQ
                     LMAFKISNYIYPIIFVGFILNFVSKKEKVKNIGMVIFSFGLLFEGIEIMGEVMKPLAG
                     SPVFVDLMGKVSSIPVLGVVLGAVMTLVVQSSSATIAVLQNFASQAGPDGVSSVIGLT
                     GAIPILLGDNIGTTITALLASIGQSKNAKRTAIAHSIFNISGSCVFIFLVPWFAKFVQ
                     FISPKGNEIDVISRQIANAHTTFNIVCTLVWLPLIPLMVKIVTTIIRGNDKTEKAAFE
                     PKYLDMKVIEQPAAAMVLVSKELNRLGELAESLLSDLKTAIVADGDSKTHGSFIENLE
                     IVHQLQDSVTEYITRLFASGNLTEQQSEQTAGLLYVNNSIQRIADRCEDIDQICEKVN
                     NNGKRLTSEASSEMEDCIDIIQQLLKKAMEAIRKGDAVVAETVFKNKKKMHKAEKKYS
                     KAHLNRVKNEVCDASMTRYFSGIMYNFDRMADNCVSIAEEACDNVAFINLDEETGKSM
                     MERGAV"
     gene            12243..13097
                     /locus_tag="IV142_000678"
     CDS             12243..13097
                     /locus_tag="IV142_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484355.1"
                     /translation="MVKKKNIRKSALLLIMIMLATGITACGTEEEPLPDLSAMGAVTA
                     VSREEGSGTRAEFENLLKLPESDTGIVVDSTEKVLKKVEEDKNAVGYVAYSSATDTNG
                     KILQINGVLPSEKTIDNNSYPLCRDYYLAYNGELTDVEQDFLTYVKSKGQDIVKQYCI
                     QADSTTTFLSDKSEGKILIEGSTSMEPMVKALADDYQKQNPNAEIEVKATDSSRGITA
                     VISGECDFAMSSRELKDYEAELLETKVIGKDAIAIVINEENPLENLTIKQLTGLYNGT
                     YKNWDDLS"
     gene            13773..14093
                     /locus_tag="IV142_000679"
     CDS             13773..14093
                     /locus_tag="IV142_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484356.1"
                     /translation="MPGKEQFMTELKMKEMQLINWFGCPNLIYTRERIHLVAMLTTDE
                     EMKKNLYRVCRFLAREEIAYRYPIMYEMIRKIFSPDDTNPPEACAMFMPDDETDDEEY
                     AMAA"
     gene            14117..14527
                     /locus_tag="IV142_000680"
     CDS             14117..14527
                     /locus_tag="IV142_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8484357.1"
                     /translation="MNKVVLMGRLTKNPEIKYAGKDNDMAVARYTLAVNRRYKRDGEQ
                     EADFISCVTFGKSAEFAQKYLHKGMRIVIGGRISTGNYKDKDGKTIYTTDVIVEEHEF
                     AQNKDNGVGADSSETPKTDKDGFMEAPEGEVPFD"
     gene            14674..14880
                     /locus_tag="IV142_000681"
     CDS             14674..14880
                     /locus_tag="IV142_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8484358.1"
                     /translation="MGVSYNRLWKMLIDKNMKRIEMQYLTGISGNILARMGKNQYVSM
                     ETIEKICKKLDCTVDEMLEFTDDE"
     gene            15227..15748
                     /locus_tag="IV142_000682"
     CDS             15227..15748
                     /locus_tag="IV142_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8484359.1"
                     /translation="MIRKAILKDVNSIEDTYNEHFQYELNHTAFTVFKKGVYPTKDDA
                     ERAIYAGALFVYEENGTIVGSIIIDKVQPIEYATIPWKEKLSEDEVMVIHLLMVRPSM
                     SGKGIASSLIKFATELAQKNSCRALRLDTGSQNIPALSLYQKNGFEIIASASKKVGDV
                     IAHKNHLFLEKIL"
     gene            15806..16231
                     /locus_tag="IV142_000683"
     CDS             15806..16231
                     /locus_tag="IV142_000683"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP8484360.1"
                     /translation="MIRELRKVDINKVAEIWLDTNIKTHYFISAQYWKSNFELVKELL
                     LQATVYVYEDKQEIQGFIGLSNEYIEGIFVSAEMQSQGIGKILLNYVKGKRNKLILNV
                     YQKNTRAISFYQREGFEIQYSGLDEATGEKDYVMAWQQK"
     gene            16410..16640
                     /locus_tag="IV142_000684"
     CDS             16410..16640
                     /locus_tag="IV142_000684"
                     /inference="COORDINATES: protein motif:HMM:NF025559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HAP8484361.1"
                     /translation="MQDDDSGFTYEISRCLYFDTCKEHGYAEFCKVFCNHDWYAYGVL
                     KHHSRFIRKSTIAEDGTVCHDTIEKVTEKHFL"
     gene            16674..16856
                     /locus_tag="IV142_000685"
     CDS             16674..16856
                     /locus_tag="IV142_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylan 1,4-beta-xylosidase"
                     /protein_id="HAP8484362.1"
                     /translation="MAKEERFEKIYSQGALTTTEIWVDKETGVNYLYHACGYSGGLTP
                     LLDRDGKPVISQAINR"
     gene            16969..18480
                     /locus_tag="IV142_000686"
     CDS             16969..18480
                     /locus_tag="IV142_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484363.1"
                     /translation="MRRSGWTKWLIVIPAMLGIVLVTYVRYTTDIWGFGAFICQLIAI
                     LELAYGLRIAMLAQNRKKSYRLTPEERHEYARYLYEKQYHRYPAVANQMLLVMARMSV
                     LLNNYERAAQELADIRIDKFNPAQLKLYYYLKVVTAMAAGDATGIQESQMCYAGIPDT
                     KCTYPSKAELAMWIEQRAAAQMAEALKKAVPDKKEHPIRISIITLVLAYSTFFYGLWY
                     GIKRDMGYEVRYRFAEISITVITISLAVLLIWGIVSLYRHNCQALSIQFGKNKVALII
                     VYGAAAIFALLIIGMSGLSVVFGMDGTETVTGCDSKYTYITVQEDYGGERRYRTNNPF
                     VMQSMDGLWWSSDTIDEEDNQEEDTTELPDLDTENSTDSEDIQSGNTIEDYETEGQHD
                     GLTIQNAMLAVYNYLQEQNTLQNMAFSYTANAKGEVYAVVSETQETKNGNTVNVRYCL
                     YDNGSKTDADGNSCEELVLEKVYPDGNYETELVDFYLVNADTMQVTDEQKNTW"
     gene            18803..19027
                     /locus_tag="IV142_000687"
     CDS             18803..19027
                     /locus_tag="IV142_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HAP8484364.1"
                     /translation="MSEKTEITFMQTRLIRLASEEWHLPVEQIIHLFKEADVLGYIEK
                     CYGIFHCEGDEAVLEDITEFLQRKGIEISA"
     gene            19020..19586
                     /locus_tag="IV142_000688"
     CDS             19020..19586
                     /locus_tag="IV142_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HAP8484365.1"
                     /translation="MLELKDGFVLYHGSYCEVKEPDLAKCAKRKDFGQGFYLTTSKEQ
                     AESFLRTSIAKAIATGTIEEGQKFGYISTFEFKLSENLETHIFENADVDWLHCIAAHR
                     KKKMFIEVEREMAKYDIIAGKIADDATNATLTAYLAGAFGTAGDKVADDFCIRQLLPN
                     KLKDQYCFKTEAAIECLKFVKGEKIWLK"
     gene            19574..19804
                     /locus_tag="IV142_000689"
     CDS             19574..19804
                     /locus_tag="IV142_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484366.1"
                     /translation="MAEVTIPKEKMNYTIDLLITMVTDEIAEETGKDRNEVLTDFLCS
                     KTGKALYDEKTKLWCTGPAYIAELYMEELKKS"
     gene            20005..20370
                     /locus_tag="IV142_000690"
     CDS             20005..20370
                     /locus_tag="IV142_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="HAP8484367.1"
                     /translation="MSRAKNPKYESMSLEELKANMEKSRQELEAAIHHKNILEQRKKL
                     VERRERSHRLIVKGAEFEKVFPLSRDLEQEDVQDMMNQLQNSSYNKSIVRQAHIAALH
                     KDQQQIAEAVKKTEKGDDS"
     gene            20370..22097
                     /locus_tag="IV142_000691"
     CDS             20370..22097
                     /locus_tag="IV142_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HAP8484368.1"
                     /translation="MALYHFSVRNVSRGKGQMVVASAAYISGQRIFDSYYNKIHDYTS
                     KSGVIFTEILTPEYVPERLTDRETLWNEVEHVERNNKAQLAYSFDIALQNELTLEENI
                     RLAKEFCQEQFVARGMIVDLAVHEGKAENEEEPDNPHFHVLVPIRPFTEEGIWGNKQR
                     REYILDEDGNRVKDAKGKDMFNAVSTTGWNDPELLKEWRRAWTEKVNGKFRECHMASR
                     IDHRSYKEQGIDLIPTIHEGYEVRAMEKKGIKTVIGELNRAIRQFNQMFISLKESIQW
                     MKTAYEEMKAELDRRQNPTLLESLQDYYDKKTQGRPPLPNFYAEMKQRGKNLSNLQEF
                     AKSISYLQTHQIETMDDLQERIEELNGVVSVSKKEISEKRKQLKELENLEKMAEVIKT
                     NQPLIDEYNHFYFQKRRERYYQQHKKEINYYRKCERELKQHLDKNGKVPTARWKREKE
                     ELQAGIEELKADNQPYQEELTFVKKVQSCADIARRDREMAEADTSGRSEEKREKQVKF
                     PTFYTVQAKEILEENGKAEQQPLNQSEQNPEKKTSLLRKLDEKKKECAERDAKQQAVK
                     KKRNYDMSL"
     gene            22127..24043
                     /locus_tag="IV142_000692"
     CDS             22127..24043
                     /locus_tag="IV142_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8484369.1"
                     /translation="MSKMQYTEEQIIRANQTDLVSFLSAQGEQLGKSGKEYRWKKHDS
                     VTVSGNRWYRHSQGRGGYPVDFVMEFYNATFPEAVKMLTGEEGEGRNSTCPAPSPDFR
                     LPEKEENNDRIIRYLTENRGIEKNMVEEWIGSGDIYEEKKHHNVVFVGRDADGIPRYA
                     HCRGTGETKYRGDVAESDKSYGFCHRGTDNQLFVFEAAIDLLSFIQLFPKDWKKRSYL
                     SLGGISSAALMAFLSERPQITSVFLCLDNDHAGNEASEKLAIEIPDGYSVIRLKPSRK
                     DWNEILCDKNADRKKSIIETVTMKVPEKEELVPMLCYEDIEQTSVEWLWFPYLPFGKL
                     TIIQGNPGEGKTYFAMMLTAACTNRKTFPNMEEIEPFNVIYQTAEDGMGDTIKPRLVE
                     AGADLSRVMVIDDTEEALTLSDDRIEKAIRQNQVRLLIIDPVQAFIGADVDMNRANEV
                     RPVFRKLGMIAEKTGCAIVLIGHLNKSSGTQSTYRGLGSIDIMAAVRSLLFIGKVKKD
                     PTTRVLIHEKSSLAPPGETMAFKLGDEEGFRWVGAYEISADDLLDGKEGKPTETKLQR
                     GTKLIYELLADGNAVTIRELDEKAKAQGISQRTMREARSRMKEELDYRMNEKQENTIR
                     LKKQGRMGDGRILE"
     gene            24728..24910
                     /locus_tag="IV142_000693"
     CDS             24728..24910
                     /locus_tag="IV142_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484370.1"
                     /translation="MAVKKKIINIRVYFDGDQDAKEVFAEVIAQKIRDTKTKDKLAIM
                     KDKQYNDYEFSNLKSA"
     gene            24937..26634
                     /locus_tag="IV142_000694"
     CDS             24937..26634
                     /locus_tag="IV142_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8484371.1"
                     /translation="MNNRYMSNNIERNATWRVGVYCRLSKDDELTGESASISNQRDIL
                     VNYCQSQGWQVVDVFQDDGYTGLNTDRPDLQRLLKACEKGLVNLVITKDQSRLGRNHV
                     QTGFLMEEFFPKHGVRYIALYDNVDTFAGDNEIAPFKNVLNEMYSRDISKKVHASYHL
                     QATKGKFTGVVPPLGYQKDPEEKGHLLIDDETAPIVRKIFQWAVDGHGINFISRELER
                     QQIPCPSWWHRKRGLRTYYTKWEKKDPENGKYVWDESYLKSLLMNPVYCGDMASQKRY
                     YRFKIGNQGDKAPEDWITVRDTHEAIIPQDVFDLVQAKMKGRKRQTEQGEYSMFAGLL
                     RCAECGGALTLKKTHTKDQHEVYTCSTYIHKGKAHCTQHRVDADDLYDAVLTRIQECA
                     KAVTGDGTELENRVKELCEEDTQGHRESLEKLVSKQKDRLETLDRLIAKLYDDLINDK
                     ITESVFDKMLEKTQKEQTDIKKELFQNESVLNTEEKLDAQSQQWIDDISEYADIKELD
                     ANLLNRLISKIVISEPQEKDGTENYCRTPETVTMEIHFNLKPIPELGTIERGSGSHK"
     gene            26659..26790
                     /locus_tag="IV142_000695"
     CDS             26659..26790
                     /locus_tag="IV142_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484372.1"
                     /translation="MLIFSRLISSKYFLIKDIKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            26882..27745
                     /locus_tag="IV142_000696"
     CDS             26882..27745
                     /locus_tag="IV142_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484373.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            27776..28318
                     /locus_tag="IV142_000697"
     CDS             27776..28318
                     /locus_tag="IV142_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HAP8484374.1"
                     /translation="MFISTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVE
                     RLASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTR
                     GNAEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFAR
                     QKTPGWDVWGNEVECDLILT"
     gene            28332..28754
                     /locus_tag="IV142_000698"
     CDS             28332..28754
                     /locus_tag="IV142_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP8484375.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            28684..31083
                     /locus_tag="IV142_000699"
     CDS             28684..31083
                     /locus_tag="IV142_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP8484376.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            31115..33106
                     /locus_tag="IV142_000700"
     CDS             31115..33106
                     /locus_tag="IV142_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAP8484377.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            33129..33380
                     /locus_tag="IV142_000701"
     CDS             33129..33380
                     /locus_tag="IV142_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HAP8484378.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            33370..34599
                     /locus_tag="IV142_000702"
     CDS             33370..34599
                     /locus_tag="IV142_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HAP8484379.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            34596..36677
                     /gene="topB"
                     /locus_tag="IV142_000703"
     CDS             34596..36677
                     /gene="topB"
                     /locus_tag="IV142_000703"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8484380.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            36826..40746
                     /locus_tag="IV142_000704"
     CDS             36826..40746
                     /locus_tag="IV142_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HAP8484381.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            40747..41691
                     /locus_tag="IV142_000705"
     CDS             40747..41691
                     /locus_tag="IV142_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484382.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            41695..41898
                     /locus_tag="IV142_000706"
     CDS             41695..41898
                     /locus_tag="IV142_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484383.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(42198..42434)
                     /locus_tag="IV142_000707"
     CDS             complement(42198..42434)
                     /locus_tag="IV142_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8484384.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(42617..42988)
                     /locus_tag="IV142_000708"
     CDS             complement(42617..42988)
                     /locus_tag="IV142_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484385.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(42985..44313)
                     /locus_tag="IV142_000709"
     CDS             complement(42985..44313)
                     /locus_tag="IV142_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP8484386.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(44274..44603)
                     /locus_tag="IV142_000710"
     CDS             complement(44274..44603)
                     /locus_tag="IV142_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP8484387.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(44861..45232)
                     /locus_tag="IV142_000711"
     CDS             complement(44861..45232)
                     /locus_tag="IV142_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8484388.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            45377..45562
                     /locus_tag="IV142_000712"
     CDS             45377..45562
                     /locus_tag="IV142_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8484389.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            46074..46292
                     /locus_tag="IV142_000713"
     CDS             46074..46292
                     /locus_tag="IV142_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484390.1"
                     /translation="MRQERPEGVHTGPACVKSHTARCIRKKEAAHFAEAWGVPNKQNE
                     NAAGGQVVDQNIRKGYSDPLLASIIGMF"
     gene            46505..47164
                     /locus_tag="IV142_000714"
     CDS             46505..47164
                     /locus_tag="IV142_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HAP8484391.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            47167..48510
                     /gene="vanS"
                     /locus_tag="IV142_000715"
     CDS             47167..48510
                     /gene="vanS"
                     /locus_tag="IV142_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP8484392.1"
                     /translation="MERKGIFIKVFSYTIIVLLLVVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQNKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLGNIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            48681..49487
                     /locus_tag="IV142_000716"
     CDS             48681..49487
                     /locus_tag="IV142_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HAP8484393.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            49505..50332
                     /gene="vanW"
                     /locus_tag="IV142_000717"
     CDS             49505..50332
                     /gene="vanW"
                     /locus_tag="IV142_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HAP8484394.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATIPEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            50329..51300
                     /locus_tag="IV142_000718"
     CDS             50329..51300
                     /locus_tag="IV142_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAP8484395.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            51293..52321
                     /gene="vanB"
                     /locus_tag="IV142_000719"
     CDS             51293..52321
                     /gene="vanB"
                     /locus_tag="IV142_000719"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAP8484396.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKFG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            52327..52935
                     /locus_tag="IV142_000720"
     CDS             52327..52935
                     /locus_tag="IV142_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAP8484397.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
BASE COUNT        15260 a        11845 c        13950 g        12200 t
ORIGIN      
        1 tttttggttt gactccttat attattgagg acaaagaaat gagtccatat tatgcacaag
       61 gaaatctgat atatgtacga ccgaaaaata caaatgagat tttagtcgga gatattatta
      121 catattatga aaattcagga aaaaaaatct cgacgcgtcg cgtaatagcc gttgaaaaca
      181 accacgaaga tttttcctca aaattttagg ttatcgctga aagccccgga aatacgggca
      241 tttcgcagga tatgagaact caatttacta ccaatttact actttcggat tgagccgcat
      301 atatgctgaa ccatactgct aatacccgcc accccaaata cggcacatca taaattgaat
      361 acgacaccgt aaacgcggcg ttcctctttc ccgtcatggg agaacaggac gccgcttttt
      421 tatgcccaaa tagagaaagg aggcgacgcc atgctattta cttccggccc tgaccgggcg
      481 tttgaacggc tgatgcagga aaagcccggt ttcgatcact acgacggcgg cggggcggac
      541 gcgccggagg attgcggcac ctgccgattt taccgcccgg attggaaata tcaattctgc
      601 cagtttgcag agtgtcccta tcagccgggc aagctgaccg actatgcagc ggtcaaattc
      661 acagtgaaag acctgctaat aaaagtcaag tagaaatttc agatttttag ggaggcgaaa
      721 aaatgcaata caaaatcaag ccgctgagca tctcaaaatt aaaacggcgg ccagccagat
      781 ggtatagagt gtaaaaatca gtcgtgctcc agggattcca agtaggcttt cacagaagcg
      841 tagtagatgc gcaggaactt gttggcagag gccatcatgt agacacgata tggcttgcct
      901 tcagagcgtt tcttgtccat gaactggtac accggctcgt ctattggagc acattgcagg
      961 atgacgctca tcaccagaaa gagcgtcctg cgcagtgagg cagatcctcg cttggaaatg
     1021 ctacggctac ggacatctat ttgaccggat tggtagggcg gggcgtcaat acccgcaaag
     1081 gcaaccaacg cttttttgga atgaaaacgg cgtacatcgc caatttcagc caggagttga
     1141 gggccgaggg ttgggccaac accaaacatc cccatcacta caggatactc cggcagagaa
     1201 gctgccaggg attgcatctc ctgtttgaga gcagctaacg cggaagaagt tgtctggagt
     1261 tgagaaatgg cctgttctac caaaagtttt gccgtatccg ttttcggtat gacaccgaag
     1321 tgtctgcaag cagaggcata aatatccagc gccttatcct cgctgaaatt gtagccgtgc
     1381 tttctgcacc acttctggta ttgggtagta aaggccttct cagaccggcc acaaacgcac
     1441 tcgcaatgcc aaaaagtagc gacaaagtcc acccacttct cgctgccatc ggcgcggggc
     1501 ggactggtaa acaagcgatt tgcgtctggg aaggtggtgt ccagcaggga gatcaggtta
     1561 ttcttcagca tggtctgaac tttggaatac tgttggtact gccggtagca gattttcagc
     1621 atgagtcggg tatcctcctc cgggatatat ctcggcaatg tgagccagtg gtcaagaccg
     1681 tagttggcca gctttacggc atcctttttg tcggttttgg cccgtcttaa actgttgttt
     1741 ccgtagtcat gcaccagcat tgcattgact accgagacat aaaggcccgc gtcgtggagc
     1801 agccaggcca ccggagcatg gtaattaccc gtggattcca tcaccacacg ggtctcgccg
     1861 tccaggcttt tgagcagtcc tgccagctcg ctcagttcat tggcggtgtg acgtacttca
     1921 aagggtgaaa ccactacctc tccgaagggc cgcatgactg caatcatgct cttacccttg
     1981 gaaacatcga tgccaacgca gttcattcac attcctccaa tacaaagaat ctgcaaccgg
     2041 aatccatctt ttctcgctgc cgattcaatc tattgtgtga cacgaactct ccgacatccg
     2101 gtggttcaac ctgcttaaat cgaacgctgc aacgagagga tggctaacag tctttcacac
     2161 ggacatacaa gcccaaggag tgattggtca gccagttgct cctctcattg tagcttaggc
     2221 acgagatgga gggaaagccg gactggctgt ccggctttcc tgtccaaatt tattgtaata
     2281 ggagcgaaca acgctatggc agttttccgt gtggaaaaga acaaaggcta tacggtgatg
     2341 tcaaatcacc acctgcggaa taagaacctg accttaaagg ccaagggcct gctgtcgcag
     2401 atgttgtcct tgccggagga ttgggactat accctaaaag gactgtccta tatcaaccgg
     2461 gaaagcatcg acgcgatcag gacagcggta tgggagcttg aaaaagctgg atatatccgg
     2521 cgtgagcagg gccgtgacgc aaaaggcaaa atggccgata tggtctatac catctacgaa
     2581 cagccggtat tggaaaaccc ggtattggaa aatccaacat cggataaccc ggtgttggaa
     2641 aatccgacat cggataatcc ggcgtcggaa aatccaacgc aattaaataa agaagtacaa
     2701 agaactgact tatcaaaaaa agaaaaaata attacagatg gacaaagtac ccattccatt
     2761 cctatcccat ccccgacccc tgcccctatg gagggcgagg cagcggctac gccgccggaa
     2821 cggaaaagga caggaaagga tgaggctgta cggatttatc gggaaatcat tttggagaac
     2881 atcgagtatg cctatctcat tcaggacagg agcattgacc gtgaacagct tgacgaaatc
     2941 gtagacctga tgctggaaac cgtctgcacc gcccgaaaga caatccgtgt cgccggggac
     3001 gactaccctg ccgagctggt gaaatccaag tacatgaagc tgaccggcga acatatccgc
     3061 tttgtccttg actgtctgcg ggagaacaca acgaaagtcc gcaatatcaa acaatacctg
     3121 cgggctatgc tgttcaacgc ccccagcacc atttccaact actacacggc ccttgtcgcg
     3181 cacgacatgg cacagcccga ttggggaaag cctaaatccg gcctgccgga ctattcctgc
     3241 ggcgagggcg aaagcctgta acgagaaaag gaggacgacc ctatgaagca gggcgcattg
     3301 atttttgacg agcgaacaga ccgttacgac atccgctttg atctggccga ctattacggc
     3361 ggcctgcatt gcggggagtg tttcgacgtg atgatcggcg gcaaatggag gcccacccgc
     3421 attgaaatgg ccgaaaactg gtatcttgtc ggcatccggg ccgacgacct ttccggcctg
     3481 cgggtgcgga tttgacctat atctacctgc ggcgtctggt ggttccgcct tagacgccgc
     3541 ttttgacttt ctgacaagga gggatgcgat tgcaggaaga agttactcaa aagaccattg
     3601 ccctgtccat gaaaacgggc aagctcacgg ctcaagtgct gcaagcggcg ctgaaaaagt
     3661 acctgcaaca ccgggccaag ggcaaaaaca cgctgcacca cggccagcag agcctaaagc
     3721 agcttaaaaa gcatggggct gatctctcca acattgagat caccgaggcc aacattggag
     3781 cgttcaagcc atgcgccaag aaatacggcg tggattttac cttacgaaaa gataagacca
     3841 cccagccgcc ccactacgtt gtgattttca aggccaagga tgcagataac cttgatctgg
     3901 cgtttcggga gtttaccgcc aagacgcttt ccaaggagca gcggccctct atccgcaagg
     3961 ttttgtccgc tgtgaagcaa aaggcggcgg cccagaccaa gcagcgggcc aaggagaaaa
     4021 tcaaggaaag ggggctggaa ctgtgaagcc tgacgtaaag aaattgctga tcctgaacct
     4081 cccgtatctg atttttgtgt acctgtttgc gaaatgcggg gaggcgtacc ggctggccgc
     4141 tggtgctgac ctgtcgggga agctcctgca ttttgcggat ggttttgcgg cggcttttgc
     4201 caacccgctg ccaagcctgc acctgtttga cctgtgtatc ggtatcgccg gtgccgtgat
     4261 cgtgcggctg gtggtgtact gcaagagcaa gaacgtgaag aaataccgca aaggcatgga
     4321 gtatggcagc gcccgctggg ggacggccaa agacatagcg ccttacatcg accccaagtt
     4381 tgaaaataat atcctgctca cccaaacgga acggcttacc atgaccggca ggcccaaaga
     4441 ccccaagaca gcgcggaata aaaacgtgct ggtgatcggt ggcagcggtt cagggaaaac
     4501 gagatttttc gtgaaaccca gtgcgcccgt aagggcggta taaatcctac cttgagcaag
     4561 tagtaggtaa aagtatcaag cggcattgtg ccgcaaccta tcattccccg tcttacccca
     4621 aaagggaaac cggagggcga ccatagcatg acggaggaca cggggcgctg aagcctcaga
     4681 agcgccggcg tgacggaatg aaaatccacg tatgaggatg gaagctagac tgcttgaatg
     4741 acagcctgaa atcgggacca agagcgtacc cctgacgtag agaggttgta ctcgtcagtg
     4801 ttcctgacag tcgcattgtt tggctatgcg gctgctgata ctcggagcag gttcagccac
     4861 gggaaagtgg aaaaaggcga acgtcacata ccgacaacgt ggaacgcttg tttataccgt
     4921 actaaacggg gattgcctaa agtgaaatgc cgaaaggcta tgaaaacgct gaaatgcggg
     4981 gttctgaata ttcaccatgg caacagagtt cccatagtag tctgcggacg ggaaagccgt
     5041 ctacatggca aaggggaaca gtgaatcatt ttcaaaacag aaaggatggt gtgtgagaca
     5101 ctatgagaaa tccgattcat gtcttgaaaa gccttgaaga aaaggcaagt gtaagtaact
     5161 acaaatatga aagattatat cgcaacctat acaatccaga gttctatctc cttgcatatg
     5221 cgaacattgc gaaatcgcag gggagcatga cgcagggggt agacgggcag acgctggaca
     5281 acatgagcct gccgagaatt aaccggatta ttgaatccat acgcaacagg acatatcagc
     5341 caaagcctgc aaaaagaaag tacattccta aaaagaacgg gaaacttcgc ccgttgggaa
     5401 tcacttctac tgatgataag ttagtgcagg aagtggtcag aatgattctt gaagcaatct
     5461 atgagccgac attcagcaac aattcacacg gtttcagacc aaaaagaagc tgtcacacgg
     5521 cacttacgca agtgaagaaa aacttcacag gtgttacatg gattgtcgag ggagacatta
     5581 aggcgtgctt tgataacttc gaccatcatg tgttggttga attactgcgg aaaaggattt
     5641 cagacgaggc ttttatcggt ctaatctgga agttcctgaa agccggatat atggaacaat
     5701 ggcagtacaa ctgcacctac tccggtgttc cgcagggcag cggtatcagt ccgatatgtg
     5761 caaacatcta cctcagcgaa ctggacaact atatgcagga atacaaagaa aagtatgatt
     5821 gcgagccaga acgcagaagg acgaccagag aatacgagcg ggcgtcccgg agatacagaa
     5881 aggcacgtaa agcgttaatg ggcgcagaaa aatcaactcc tgaactggta aaagaattta
     5941 aggattccag aaggaagaag atgaatcagc actattacaa tccgtttgag gaaggcttca
     6001 agaaaatcca gtacaaccgt tacgccgatg attttgtaat cggcgttatc ggctccaaaa
     6061 aggacgcaga aaaaatcaag gaagatgtaa aaatctttct ccaagaaaaa ctgcatttgg
     6121 aaatgtccga ggaaaagacg aaagtcaccc attccagtaa gccggtacgc tatctggggt
     6181 acgatttcaa agtaatccat tccaagaaca tgaagcgttg taaaaacggc gacatgaaac
     6241 gggtgtggta tggaaaagta ttcctgtata tgccgaaaga aaaatggatt aaaaaagcga
     6301 tggaacgggg agcgatacag gtgaaacgca acaatgacac aggcaaagaa atgtggcggc
     6361 ctatgccaag gaaagacctg atgaaccgca gcgacgcaga gattgtgtct actttcaatt
     6421 ccgaaattcg agggttatat aacttctatc ggatagcaga aaacgtaggc gcattgcaca
     6481 agtattacta catggtgcgg tacagcatgt taaaaactct ggcaggaaag cacagaacga
     6541 atgtcagtgt tattaagaag cggcacatgg taaacggagt actgagaatc ccatacgata
     6601 caactaaggg acgtaaatac tgcgaatttt accatgatgg attcagaaag cacagcgacg
     6661 gctatgacaa tgtggcagac gttatgccga gttataggaa atatgacagc aggcacacga
     6721 tagtaaaccg cataaaagcc ggagtatgcg agatttgcgg ggaacatgca gactatttat
     6781 gtatgcacca cgtaagaacg ctgaaatcgc tgaaaggcag ggatatattt gagcagaaaa
     6841 tgctgaaaat gagaagaaaa tctctggctc tctgccctga ctgctttgaa ctcttacacg
     6901 aaaccaaaga atcaaggtga ttcatggaaa gccggatacg ctgagaggtg tacgtccggt
     6961 ttgggaggcg gctccctgaa acctactgat gaaagtcagc aaggcgcagg gtgcctacct
     7021 catacctgat gcaatgcttt ccgaccacgg actatccgac ttcgtttgtg gtgacagatc
     7081 cgaaaggcac tttggttctg gaaaccggca ggatgttcca gcaggcgggc taccgcatta
     7141 aaatcctgaa taccattaac ttttccaagt ccatgaagta caaccccttt gtctacatcc
     7201 attcggaaaa ggacgtgcta aagctggtaa ataccctgat ttccaacacc aagggcgagg
     7261 gggaaaagtc agcggaggat ttttgggtga agtcggaacg gttgttttat tcggccctga
     7321 tcggctatat ctggtacgag gccccagcgg aggaaatgaa cttctcaacg ctgctggaaa
     7381 tgataaacgc cagtgaagcc cgcgaggacg acccggagtt tcaaagcccc gtcgatctca
     7441 tgtttgagcg tttggagcag aaagaccccg accattttgc ggtgcgccag tacaagaaat
     7501 tcctgctgtc cgcaggcaag acccgttctt ccatcctgat ttcatgcggt gccagacttg
     7561 cgccctttga catccgggag gtacgcgagc tgatggagga cgacgaaatg gagctggaca
     7621 ccatcgggga cgaaaagacg gtgctgtatc tcattatgag cgatacggac acgaccttta
     7681 atttcattct ggctatgctc caaagccagc taatcaacct gctttgtgac cgggctgatg
     7741 atgtgtacgg cgggcggctg cctgtccatg tacgcctgat tttggatgaa tttgccaaca
     7801 ttggccaaat acccaatttc gataaactca ttgccaccat tcgaagccgt gaaatatcgg
     7861 cctctattat tttgcagagc cagtcgcagc taaaagcgat ttacaaggac gccgccgaga
     7921 tcatttcgga taactgcgat agtgtgcttt tccttagtgg gcgcggcaaa aatgccaagg
     7981 agatttccga tgcgctgggg aaagaaacga ttgacagttt caacaccagc gagaaccggg
     8041 gaagccaaac gtcccacgga ttgaactatc aaaaattagg aaaggagttg atgtcccaag
     8101 acgaaattgc cgtgatggac ggcggcaagt gtatcttgca gctacggggt gtgcgcccgt
     8161 ttttatcgga gaaattcgat attaccaagc atccccggta caaatacctt gccgacgccg
     8221 acaagaagaa tacctttgac gtggacagat ttttgactat cacgcgccgg aaacggcagc
     8281 aagtggtaac gcaggatgaa gtattcgacc tgtatgaaat cgacctgtcg gacgaggacg
     8341 ccgccgctga ataaacagcg gcttttttca ttccaacaac aaaatcaaaa aatcattatg
     8401 agccggaaac cggcagaaag aggtaaattt atggcatttt ttaattccgc agttactact
     8461 ttgcagacta tcgtgatcgg actgggcggc gctctgtgcg tatggggcgg catcaatctg
     8521 ttggagggat acgggcagga caatccgggg gcaaaatcgc agggtgtcaa gcacttaatg
     8581 tcttctatgt gaaaagattt taaagagaaa atgcacatcc tacgcagata acatgacaat
     8641 ttcatgcaaa gcgaaaggag acaccatgaa aatcgaatac acaaaagtgg gagactatta
     8701 tttaccgaat ctttactacc cggaggaaac cagaccgatt ggcttatggg gaatgcttcg
     8761 gaaggaatat ctgaaagaac ataaatctgg aacttataca tatcttcttc tgaccgccag
     8821 gctggacagt tatttggcag atctgaatga acaggcacag gaacgctttg aattgatcga
     8881 aactcagatg cgaagtgcgg agggagtttc cgaggaactg aagagacaga atcctatgga
     8941 atggctatgc cactgtaaca atatccgcaa ccgggctgct gagatcatca agcaggaact
     9001 gatttatgta taagggggat tctagatatg agaagagaac tgattgaaag ttattatgaa
     9061 gaataccagc gggagctgga aagatttctt gaatctgacc cggagatgtt cactctggaa
     9121 gcagaaattc atcattacat agaaatactg gaaccggtcc tcagagatgg cggacaggag
     9181 atgtggcaga cactggacag gattatttca gcgaaaaatg caatggaggg aattgtggca
     9241 aaagaaatgt atatccgggg atttctggat tatgaaagac tggtgtgtga aaaagacagt
     9301 aagatctgaa taatcagaac atagactata tataacgagg cttttattgt atagacagta
     9361 agagcctcat ttaatatagt tttcataata aaaaacaggt ggtagtacgt atttgctttg
     9421 tgttatataa gaattggtct ggctgaacaa tggtgaaaat ttactatgat tttacttaaa
     9481 tctgattgaa ggatgaaaaa gtgatgatat catatagtgg gttgataggg ttacagaaaa
     9541 tgtattcaca gaggcagaga agaaataatg aaagtaaaaa atgcagatga attttataaa
     9601 atattagaaa gaaaggctat atatccggta tttcagccga ttgtaaattt acagactgga
     9661 gatgtggcag gatatgaagc tcttagtcgt atcgacaggc atgatacgac tcttatgatt
     9721 tcagatttgt ttgtgattgc tgaacaggta ggctgtgttt ggaaattgga aaaattgtgc
     9781 agaaataaag cattgaaggc agcggcgaat aaaccagaac atgcaaaatt atttcttaat
     9841 gtagatggta atatcattca ggataaatcg ttcatacagg gatttacaaa ccgtaaggct
     9901 gcgaaagccg gagtaccgtc ctgtgacatt gtatttgaaa taacggaacg gtcggatata
     9961 gaaaactatc agattctgca acagattatg aagcattatg cagatcaggg atatgaaata
    10021 gctttggatg atgttggagc tggatattct ggtctgaacc gtgtagtaaa tacatcgcca
    10081 aactacttaa aagttgatat agaactggtc agagatattc aaaaacataa gaaaaaggaa
    10141 attatgatgg aatttctgtt gcattactgt aatgagacag gagcaattct gattgcagaa
    10201 gggatagaaa cagaagcaga gttggagtgt ctgtgtagat taggtgttca ttacggacaa
    10261 ggatattttc ttggaagacc ggacagaacg ttcaaaaatg taaggaatga ggcgatgttg
    10321 gtattacgta gactgaataa taaatcgtaa tgaaaactat atgtcagcag gtgaaaaatg
    10381 aataagtcaa ttataacatt ataaagattt ccattgattt ttacacctat tttacacaag
    10441 cactcacata gatttgattt ctgtcttcta taattgtata ggaaaatgta gatcttatat
    10501 aataacagga gaggaaagag aaatgaacga gaatgtgaaa gtggtttttg gtctgatcgg
    10561 tggccttgcc ttgttccttt atggaatgaa cagcatgagt gatgcacttc agaaagccgc
    10621 aggagaacga atgaaaaaaa tccttggatt tcttacaaga aatccaatta tgggagcact
    10681 tgccggagca cttgtaacag ctgtattgca gagcagttca gcgacgacag ttatggttat
    10741 cggttttgtc agtgcgggac ttatgagtct tccgcaggca atttctgtta tctttggtgc
    10801 caacatagga accactatga cggcacagct gatggcattt aaaatcagca attatattta
    10861 tccaattatc tttgttggat ttattctgaa ctttgttagc aaaaaagaaa aggttaaaaa
    10921 tatcggtatg gtaatttttt cattcggact tttatttgaa ggaattgaaa tcatgggtga
    10981 agtcatgaaa cctttggcgg gaagtccggt ctttgttgat ctgatgggaa aagtttcatc
    11041 cattccggtt cttggtgttg tactgggagc ggtcatgaca ttggttgtgc agagtagttc
    11101 agctacgatt gcagttctgc agaattttgc atcacaggca ggaccggacg gagtaagcag
    11161 tgtaataggg cttaccggtg cgattccgat tcttcttgga gataatatag gtacaacgat
    11221 cacagcactt cttgcatcaa ttggacaatc taaaaatgca aaacgtacag caattgcgca
    11281 tagtattttt aatatcagtg gaagctgcgt ttttattttc ctggttccgt ggtttgcaaa
    11341 atttgtccag tttatttcac caaagggaaa tgagattgat gtgatttcaa gacagattgc
    11401 aaatgcacat acaacgttta atatcgtctg tacactggtc tggctgccac tgattccgct
    11461 gatggtaaaa attgttacaa caatcatccg tggaaatgac aaaacagaga aagcagcttt
    11521 tgaacccaaa tatctggata tgaaagtgat tgaacagccg gcggctgcta tggttctggt
    11581 atctaaggaa ctgaaccgac ttggagaact ggcagaatct ctgttaagtg atttaaaaac
    11641 ggcgattgta gcagatggag actcaaaaac tcatggttcg tttatagaaa atctggagat
    11701 tgtacatcag ttacaggaca gtgtgacaga atatattacc agattattcg caagcggaaa
    11761 ccttacagaa caacagtctg agcagacggc agggttactg tatgtaaaca atagtattca
    11821 gaggattgca gacagatgtg aagatattga ccagatatgt gaaaaagtaa ataacaatgg
    11881 aaaaagactg accagtgaag cctcctcaga aatggaagac tgcattgata ttatccagca
    11941 gcttttaaag aaagcaatgg aggcaatccg taagggtgat gctgtagtgg ctgaaacagt
    12001 ttttaaaaat aagaaaaaaa tgcataaagc agagaaaaaa tacagtaagg cacatttaaa
    12061 ccgtgtaaaa aatgaggttt gtgatgcgtc tatgacaaga tatttttctg ggatcatgta
    12121 taattttgac cgtatggcag ataattgtgt cagcattgca gaagaagcct gtgataatgt
    12181 ggcttttata aaccttgatg aagagacggg aaaatcaatg atggaaagag gtgcagtata
    12241 aaatggtaaa gaaaaaaaac atccgaaaat ctgcacttct gctgataatg ataatgctgg
    12301 caacgggaat aacagcatgt ggaaccgagg aagagccatt gccggatctg tctgctatgg
    12361 gagctgtcac agctgtatca cgagaagaag ggtcaggaac cagagcagaa tttgagaatc
    12421 ttttgaaact gcctgagagt gatacaggaa tagttgttga ttctacagag aaagttctga
    12481 aaaaggtgga agaagataaa aacgcagttg gttatgtcgc atatagttcc gcaacagata
    12541 cgaatggaaa aatacttcaa atcaacggag ttcttccttc tgagaaaaca atagacaata
    12601 acagttatcc tctttgcagg gattattatc tggcttataa tggagaactg acagatgtgg
    12661 aacaggattt cctgacttat gtaaagagta aaggacagga tattgtgaaa caatattgta
    12721 ttcaggctga cagtacaaca acattccttt ctgacaagtc agaaggaaag attctgattg
    12781 aaggatccac ttcaatggaa ccaatggtaa aagcactggc agatgactat cagaaacaaa
    12841 atccaaatgc tgaaatagaa gtaaaagcaa cagattcttc aagagggatt acggcggtga
    12901 taagtggaga gtgtgatttc gcaatgtcat ccagagaatt aaaggattat gaagcagagc
    12961 ttctggaaac aaaagtcatt ggaaaagatg ctatagctat tgtgataaat gaagaaaatc
    13021 cactggaaaa tcttacaata aaacaactta caggtctgta caatggaaca tataaaaact
    13081 gggatgatct ttcctgagaa aaacgaatat aatatcttac aagcactgac tataacggtc
    13141 ggtgcttctt ttttggattt tttcaaaatt atctgacatt cttcaaattc ccacgtgtga
    13201 ttagtggaaa aataattttt tctgcaaaag taccgtccaa atgccctctg aaacttgcta
    13261 ttagtgaggg aatatttcat ttatgcgaaa aaaacttgca aaagggggtt ggtaaatacc
    13321 gcttcccagg ctttataggt agaggaacaa ttattttaaa aattttaccc caaagatgtc
    13381 cgcttttgcc tgtctcagta cagagtatgt gaagggacta acaaaagtaa aagaaaaaat
    13441 gataaaaatg caaaaaaata tcacgaatct tttgcatctt gcgaagtcat ttgactctag
    13501 taatcagagg acgttttgga atgttccttc gatgaacctt gacaaccgat aagtgcaccc
    13561 tagaagccta acaggagtta tgccatgacc tttctttttc tgagagcgag cgaaattata
    13621 atgacactcc cgatgatggg cggctgaccg aagccttatg gtcagtgacc tgctgatgca
    13681 ggcagaggca gtctgagacg gctgcaggtg acagggaaac gcacgccaga ccgccggacg
    13741 aatgaaactg atgacaatgt ttacagggca aaatgcccgg aaaggagcag tttatgacag
    13801 aactgaaaat gaaagaaatg cagttgatta actggtttgg atgcccgaat ctgatttata
    13861 caagggagag aatccatctg gttgccatgc ttacaacaga tgaagaaatg aaaaagaatc
    13921 tttacagggt atgcagattc cttgcaagag aagaaatcgc atatcggtat ccgatcatgt
    13981 atgaaatgat ccggaaaata ttttcaccgg atgataccaa tccaccggaa gcatgtgcaa
    14041 tgtttatgcc ggacgatgaa acagatgatg aagaatatgc aatggcagcg tagctgcgga
    14101 aaggacagta acgattatga ataaagtggt tttaatggga agactgacaa agaatccgga
    14161 gatcaaatat gccggaaaag ataatgatat ggcagtagca agatatacac tggcggtaaa
    14221 cagaagatat aaacgtgacg gtgaacagga agcggatttt atttcctgtg tcacattcgg
    14281 aaagagtgca gagtttgcac agaaatatct tcacaaggga atgaggattg tgattggcgg
    14341 tcgtatcagc actggcaatt ataaggacaa ggacgggaaa acaatttata ctacagatgt
    14401 gatcgtggaa gaacatgaat ttgcccagaa caaggataat ggtgttggag ctgattcatc
    14461 ggaaacaccg aaaacagata aggacggatt tatggaagca ccggaaggtg aggttccatt
    14521 cgactaaacc gaagtaaatg gttggagagg ttgccctttg tcggggcatc ctcttttcca
    14581 ttttacagaa gacatcaaaa attacaggta ttcaacctaa cgaaatcgtg atataatcaa
    14641 aatatgttca gatgaaaaat atggaggttg cttatgggcg taagttataa cagattatgg
    14701 aaaatgctca tagataaaaa tatgaagcgg atagagatgc agtacctcac cgggatcagt
    14761 ggaaatattc ttgccaggat gggaaaaaac cagtatgtat caatggagac gattgagaag
    14821 atctgcaaga agctggactg caccgttgat gagatgctgg aatttacaga tgatgagtaa
    14881 caagagcaga catgtcaaat tattaaaact ggaagaatgc ttcagaagtc gaaaaaagaa
    14941 attgaaaaaa gataacacgc cggatacggc ataaatctct tagcaacgct aagagcgcac
    15001 ttctatacag gcaattccat ctgcctgtat cgtgcgacct gcggtgctgt tcccgatttc
    15061 aagaaatctc aaaccgggaa atacagcgaa atcagagatt tcgtacaagg ggaagatccc
    15121 cttgcggcac agatgcctat ccctgataaa gtttgtgact gaatatttca gatatagaca
    15181 tataagggga aagaaagtgt gaaatcaaaa tttgtggaga gattttatga ttagaaaagc
    15241 aatactaaaa gatgttaatt cgattgaaga tacttacaat gagcatttcc agtatgaact
    15301 gaatcataca gcctttactg tatttaagaa aggggtttat ccaacgaaag atgatgcaga
    15361 acgggctatt tatgctggtg ctttgtttgt atatgaagaa aacggaacaa ttgttggaag
    15421 tatcattatt gataaagttc aaccaataga atatgcaact attccttgga aagaaaaatt
    15481 atctgaagat gaggtaatgg taattcacct tttaatggta cgtccgagta tgtctggtaa
    15541 aggaattgca tcatcactaa taaaattcgc tacagaatta gcacaaaaaa atagttgcag
    15601 ggcactacgt ttggatacag gaagtcaaaa tattcctgcg ctatctttgt atcaaaaaaa
    15661 tggatttgaa ataattgcta gtgcttctaa aaaagttgga gatgtaattg cacacaaaaa
    15721 ccatcttttt ctcgaaaaga tattgtagtt cgtaaattcc agtttgaaaa attgagaaaa
    15781 tcggagtttg aggagcatca ccgttatgat tagagaatta cggaaagtgg atataaataa
    15841 agttgcagaa atatggttag atacaaatat aaagacacat tattttatct ctgctcaata
    15901 ttggaaaagt aattttgagt tagtaaaaga acttctatta caggcaactg tctatgttta
    15961 cgaggataaa caggaaatac agggttttat agggttaagt aacgaataca ttgaaggtat
    16021 ttttgtttct gccgaaatgc aatctcaagg tataggtaaa attttgctaa actatgtaaa
    16081 gggaaaaaga aacaaattaa tcttaaatgt atatcaaaaa aatacacggg caatatcctt
    16141 ttatcaaaga gaaggatttg agattcagta tagcggtttg gatgaagcta ctggagaaaa
    16201 agactatgta atggcatggc agcagaaata gaaattcaag ttttagtgtt tggaaaatta
    16261 tgacttagtg tgcaagttgg gagaaaatat ggtaacggaa aaagagaaaa aagcatgtcg
    16321 atttattgcc gaatggctat aagattgtga gacagtctct ggtagatgat ctggagggcg
    16381 aaaaaaaggt atgttgggag acagaaattt tgcaggatga tgatagtgga tttacttatg
    16441 aaatcagcag gtgtctgtat ttcgatacct gtaaggaaca tggctatgca gagttctgca
    16501 aagtattttg caaccatgac tggtatgctt atggagtatt aaagcatcat tcgagattta
    16561 tcagaaaatc gacgattgcc gaagatggaa ctgtatgtca tgatacaatc gagaaagtaa
    16621 cagagaaaca ttttttataa aaccgaaaaa ttgattatgg gaggaaattg aaaatggcga
    16681 aagaagagag atttgaaaaa atatattctc agggagcatt aactactact gagatatggg
    16741 tggataagga aactggtgtg aattatttat atcatgcatg tgggtattca ggtggactga
    16801 ctccattgtt ggacagagat gggaaaccag ttatttctca ggctataaat agatgacctt
    16861 agaaacagca actgttatgg taagtaacag catcgtcaca atatgtatat ggtcgattgg
    16921 tgtgtgcggt aatatactat attcagaaaa acaattcagg agatttttat gagaagatca
    16981 ggttggacaa aatggttgat cgtgattccg gcaatgctag gcattgtgtt ggtgacctat
    17041 gtgcggtata caacggatat ctggggattt ggtgcattta tctgccagct gattgcaatt
    17101 ttggaattgg cttatggtct gcgaattgcg atgctggcac agaatagaaa gaagtcctat
    17161 cggttaacac cggaagaacg acatgagtat gcacggtatt tatatgagaa gcaatatcac
    17221 cgttaccctg ctgtagcgaa tcagatgctt ttggttatgg cacggatgag tgtactcctt
    17281 aacaattatg aacgggctgc acaggagctg gcagatattc gtatagataa attcaatcca
    17341 gcacagttga agttgtatta ttacctgaaa gtggttacag caatggcagc aggagatgcg
    17401 acagggattc aagagagcca gatgtgttac gcagggattc cggatactaa atgcacatat
    17461 ccgtcaaaag cagagcttgc tatgtggata gagcagagag cggcagcgca gatggcagag
    17521 gcactgaaga aggcagtgcc ggataaaaaa gagcatccga tacggatcag tatcattact
    17581 ttggttcttg cgtatagtac cttcttctat ggattgtggt atggtataaa acgtgatatg
    17641 ggatatgaag tgagatatcg tttcgcagaa atatcgatta ctgttattac aatcagtctg
    17701 gcggttcttt tgatctgggg gatcgtgagt ttatacagac acaattgcca ggcattatcc
    17761 atacaattcg gtaaaaacaa agtggcattg ataattgtct atggagctgc tgcgatattt
    17821 gcacttttga ttattggaat gagtggtctg agtgtagtgt tcggaatgga tggaacagaa
    17881 acggtgactg gttgtgacag caaatatacg tatataactg tgcaggaaga ttatggtggt
    17941 gaacggagat acagaaccaa taatccattt gttatgcaga gtatggatgg tctgtggtgg
    18001 tcttcggata caatagatga agaagataat caagaagagg atactacaga attaccggat
    18061 ttggatacag agaattcaac agattcagag gatattcaga gtggaaatac catagaagat
    18121 tacgagacag aagggcagca tgatggtctg acaattcaaa atgcaatgct ggcagtttat
    18181 aattaccttc aggagcagaa tacacttcaa aatatggcgt tcagttacac ggcgaatgca
    18241 aagggagaag tctatgctgt cgtcagcgaa acgcaggaaa caaagaacgg taatacggta
    18301 aatgtgagat actgtctgta cgataatggt tccaagacag atgcagatgg taattcctgc
    18361 gaagaactgg tgttagaaaa agtatatccg gatggcaatt acgagaccga attggtagat
    18421 ttttatctgg taaatgcaga tacgatgcag gtgacagacg aacagaagaa tacgtggtaa
    18481 aataaattct ggggaagtag gtgcattgta tgacaatgag agaaaaaata aaactattat
    18541 cgtaaatgtt tcaagcggac tgggttcttt tggaagtatg caaaaggact ccaagacata
    18601 agaggtgaac tgcgctgatc caggtgccac ggcaacagat ttgaatgtcg acggaggaaa
    18661 gcaaacagta caggcgcaat cgtaaaactt gcaatgattg ataaaaccgg actaacagta
    18721 acttttatta gtcgatttgg aaatatgcct tggtaatcaa caattccggt tttactgact
    18781 aagaaaatga ggaggattca taatgagtga gaaaacagag attactttta tgcaaacaag
    18841 actaatccgg cttgcttcag aagagtggca tttacctgtt gaacagatta tccacttgtt
    18901 taaagaagcc gatgttcttg gatatataga aaaatgctat gggatttttc attgtgaagg
    18961 cgatgaagct gtgcttgaag atattactga atttttgcaa agaaagggga tagagattag
    19021 tgcttgagtt aaaagatgga tttgttctct atcatggaag ttattgtgaa gtaaaagagc
    19081 cagatcttgc caaatgtgca aagagaaaag attttggaca gggattttat ttaaccacat
    19141 caaaggaaca ggcagaaagt tttctgagaa cctcaatagc caaagccatc gcaaccggaa
    19201 caattgaaga ggggcagaag tttggatata tttcaacttt tgaattcaaa ctttcagaaa
    19261 atcttgaaac gcatattttt gaaaatgcag atgtggactg gttacattgt atcgccgctc
    19321 accgaaagaa aaaaatgttt attgaggttg aacgtgaaat ggcaaaatat gatatcattg
    19381 caggaaagat tgcggatgat gcaacaaacg ctactcttac cgcttatctt gcgggtgctt
    19441 ttgggactgc aggtgataaa gtagcagacg atttctgtat cagacagttg ctgccgaata
    19501 aattgaaaga tcaatactgc tttaaaactg aggcagctat tgagtgtctg aaatttgtga
    19561 aaggtgagaa gatatggctg aagtaacaat tccaaaggaa aaaatgaatt atacaatcga
    19621 ccttctgatc actatggtga cagatgaaat cgcagaagag acaggcaagg acagaaacga
    19681 ggtattgaca gattttttgt gttcaaagac aggaaaagca ctgtatgatg agaagacaaa
    19741 gctgtggtgc actggtccgg catatatagc tgaactgtat atggaagaat taaagaaatc
    19801 ctgatatggg atgtgagttt aaatttatac gacaatggtg acaaagactt tcattccaag
    19861 gagcaacaat taaatatttc aaaaacgctg aggtcagcat ttcctaatca caggacaacg
    19921 acctcagttt tttataccca ggaatcggca ttcatgaata agccggttct ttttgtttac
    19981 agaaaaaaga aggagtacaa gaaaatgagc agagcaaaaa atccaaagta tgaatccatg
    20041 tcattggagg aactgaaggc caacatggaa aagagcagac aggaactgga ggcagcaatt
    20101 caccacaaaa acattctgga gcagcggaag aagctggtag aacgcaggga acgttcccac
    20161 cggctgattg tcaaaggggc agagtttgag aaagtatttc cgctgtccag agatctggaa
    20221 caggaagacg tccaggacat gatgaatcaa ctgcagaaca gcagttacaa caagagtatt
    20281 gtgcggcagg ctcatatagc cgccttacac aaagatcaac agcagattgc agaagcagta
    20341 aaaaagacag agaagggaga tgacagttaa tggcactgta tcatttttca gtaaggaatg
    20401 tatccagagg gaaagggcag atggtggtcg cttccgcagc ctatatttcc gggcagagga
    20461 tttttgacag ttattacaac aagatacacg attatacgag caagtcaggt gtgatattta
    20521 cagaaatcct gacaccggag tatgtgccag agcgtctgac tgacagggaa acactctgga
    20581 acgaggtgga gcatgtggaa cggaacaaca aggcacagct tgcctattcg tttgacattg
    20641 cactgcagaa tgaattgaca cttgaggaaa atatcagact ggcaaaagag ttctgccagg
    20701 agcagtttgt ggcacgtggc atgatcgtgg atctggcagt ccatgaggga aaagcggaaa
    20761 atgaagaaga accggataat ccccatttcc atgtgcttgt cccaatccgt ccttttacag
    20821 aggaaggaat ctggggcaat aagcagagaa gggaatatat tcttgatgag gatggaaacc
    20881 gggtgaagga tgccaaagga aaagatatgt tcaatgctgt ttctactacc ggatggaatg
    20941 atccggagct tttaaaagaa tggcggcggg catggacaga gaaagtaaat gggaaattca
    21001 gggagtgcca catggcatcc agaatcgacc accgttccta caaagaacag gggattgacc
    21061 tgatcccgac catccatgag ggatatgaag tccgggcaat ggagaaaaag ggaatcaaaa
    21121 cggtgatcgg agaattgaac cgggcaatcc ggcagtttaa ccagatgttt atttctctga
    21181 aagaatccat tcagtggatg aagacagctt atgaggaaat gaaagcagag ctggatcgca
    21241 ggcagaaccc gacactgctg gaaagcctgc aggattatta tgataaaaag acacagggca
    21301 gaccaccact tcccaatttt tatgcggaga tgaaacagag aggtaagaat ctctccaatt
    21361 tacaggagtt tgcaaaatcc atcagttatc ttcagacaca tcagatagaa acgatggatg
    21421 acttgcagga acggattgag gaactgaacg gtgttgtttc tgtcagtaaa aaagaaattt
    21481 ctgaaaagcg gaaacagtta aaggaacttg agaatctgga aaagatggca gaggtaataa
    21541 aaacaaatca gccattgatt gatgagtata accatttcta ttttcagaaa agacgggaga
    21601 gatattatca gcagcataaa aaagaaatca attattaccg caaatgtgaa cgggaactga
    21661 aacaacatct ggataaaaat ggaaaagtgc caactgccag atggaaacgg gagaaagaag
    21721 aacttcaggc ggggatcgaa gagctgaaag cagataatca gccgtaccag gaggaactaa
    21781 cttttgtgaa aaaggtacag agctgtgcgg atattgccag acgtgaccgg gaaatggcag
    21841 aagcagatac ttccgggcgt tcagaagaaa aaagggagaa gcaggtgaag ttcccgacat
    21901 tttacacagt tcaggcgaaa gagattcttg aagaaaacgg gaaagcagag caacagccgt
    21961 tgaaccagtc ggaacagaat ccagaaaaga agacctctct tctgagaaaa ctggatgaaa
    22021 aaaagaaaga gtgtgcagag cgtgatgcaa aacaacaggc agtgaagaaa aagcgaaact
    22081 atgacatgag tttataacag tctgtcagca ggacagacgg gaaaggatga gcaaaatgca
    22141 gtatacagaa gaacaaatta taagagcaaa tcagacagat cttgtttctt ttctgagcgc
    22201 acagggagaa caactaggga aaagcggaaa ggaataccgt tggaaaaagc atgacagtgt
    22261 aacggtatcc ggaaaccggt ggtacaggca cagtcagggg aggggtggct atccggtgga
    22321 ttttgtgatg gaattttaca atgccacatt tccggaagct gtcaagatgc taaccgggga
    22381 ggaaggcgaa ggcaggaaca gtacctgccc cgccccgtcc cctgacttcc gattaccaga
    22441 gaaagaggaa aataatgata gaatcatcag atatctgaca gaaaacagag ggattgaaaa
    22501 gaatatggtg gaggaatgga tcggcagtgg ggatatttat gaagaaaaga agcaccataa
    22561 tgtggtgttt gttggcagag atgcagacgg tattccaaga tatgcccatt gcaggggaac
    22621 tggtgaaaca aaataccggg gagatgtggc tgaatctgat aaatcttacg gattctgtca
    22681 cagagggacg gataatcagt tgtttgtgtt tgaagcagcc attgatctgc tttcttttat
    22741 ccaattattt cccaaagact ggaaaaagcg gagctacctg agtctgggtg gcatttccag
    22801 tgcggcactg atggcttttc tttctgaacg ccctcagatt acttcggttt tcctgtgcct
    22861 ggataatgac catgcaggga atgaagccag tgagaaattg gcaatagaaa tcccggacgg
    22921 atacagtgtc atccgtctga aaccgtcccg aaaggactgg aatgaaatcc tctgtgataa
    22981 aaatgcagac aggaagaaat caatcattga aacagttaca atgaaagtac cggagaagga
    23041 agaactggtg ccgatgttgt gttatgaaga cattgaacag accagtgtag aatggctgtg
    23101 gtttccctat ctgccatttg gaaaactgac gattattcag ggcaatccag gagagggaaa
    23161 aacttatttt gccatgatgc tgaccgcagc ctgcacgaac cgtaagactt tcccgaacat
    23221 ggaagagatt gaacctttta atgtgatcta tcagactgca gaagacggta tgggagatac
    23281 gattaagccg aggctggtgg aagcaggagc agacctgagt cgggtgatgg tcattgatga
    23341 tacagaggaa gcattgacgt tatccgatga ccgtattgaa aaagcaatcc gtcagaatca
    23401 ggtcagactt ttaatcattg atccggtaca ggcttttatc ggtgccgatg tggatatgaa
    23461 ccgggcaaat gaagtccgtc cggtattccg gaaactggga atgatcgctg aaaagaccgg
    23521 ttgtgcgatt gtgctgatcg gacatctgaa taaatcttca gggacacaga gtacttaccg
    23581 gggacttggt tctattgata tcatggcggc ggtgagaagc ctgcttttta tcgggaaagt
    23641 aaagaaagac ccgacaacaa gagtactgat ccatgagaaa agttctcttg cacctccagg
    23701 tgagacgatg gcatttaaac ttggagatga agaaggattc cgctgggtgg gagcttatga
    23761 gatttccgca gatgatcttc tggacggaaa ggaaggaaag ccgacagaaa caaaactgca
    23821 acggggaacg aaactgatct atgaactgct tgcagacgga aatgcagtca caatccggga
    23881 actggatgaa aaggcaaaag cacagggaat ctcccagaga accatgcgag aagccagaag
    23941 ccggatgaaa gaagaactgg attatcggat gaatgaaaag caggaaaata ccattcgatt
    24001 aaaaaaacaa gggagaatgg gtgacggcag aattttggag tgaacggcag gctttataga
    24061 taccctatct gccgtctgcc gtctgccggt gcaaggcaat caaacggaaa tagcaggtat
    24121 ccatacagaa taaggattgc tgtgaatctg gaacaaaaat gaattgtaat atggcggata
    24181 taatggtatg attatccggt aacagcaatg ttacggggaa gcacctgtga cagggtgtgg
    24241 ctgcctcctg atatttacag accggagcct ttcggggttc tgagatgaaa gaaaggaggt
    24301 gcgtgatatg acagcttatg agattatctc aatattctta ggcgtactca gtttactgat
    24361 cgcttttggt agcctgttaa ttgcgattat tgcctttctt gatcggaaga acaagaggaa
    24421 ataaaaatgc ctatcctgtc tgccaacagg ataggcggtg gtcgtaagac catttagtca
    24481 actcaggcgg catccacttt ggagtgggtg cttttcctat aacaaatgta ccacacattc
    24541 ctcttgtttt caatgggaaa attaaaatat gcagttcatc tgacagggat ttatccctgt
    24601 ctttattata tccccaattt ctgaaatgtt aatacaaaaa ttttaatata tgtaaccaac
    24661 agtctgggca gttaatctgt aaaaatggat tagctgctct ttttttgcaa aaaatggagg
    24721 ttttacaatg gcagtgaaga agaaaattat caacatcaga gtatattttg acggtgatca
    24781 ggacgcaaaa gaagtgtttg cagaggtgat cgcacagaaa atacgtgaca cgaaaacaaa
    24841 agataaactt gcaataatga aagataagca gtataatgat tatgagttca gtaatctgaa
    24901 atctgcgtag gatgtgcaaa gtaacggagg tatcagatga acaacagata tatgagtaac
    24961 aatatagaaa gaaatgcaac ctggagagtt ggtgtatact gccgactttc caaagatgac
    25021 gaattgaccg gagagagtgc cagcatctcc aaccagagag acatactggt gaattactgt
    25081 cagtcacagg gctggcaggt ggtggatgtg tttcaggacg atggttatac aggattgaac
    25141 acagaccgac ctgatttgca gagacttctc aaagcctgtg aaaaaggact ggtgaatctt
    25201 gtaattacca aagaccagag ccgactggga cgaaaccatg tacagacagg atttctgatg
    25261 gaggagtttt tcccaaagca tggagtaagg tatatcgcat tatatgataa tgtggatacc
    25321 tttgccggag acaatgagat tgccccattt aagaacgtgc tgaacgagat gtattccaga
    25381 gatatttcca agaaagtcca tgcgtcctat cacctgcagg caaccaaggg aaagtttaca
    25441 ggtgttgttc caccactggg atatcagaaa gacccggagg aaaagggaca tctcctgatt
    25501 gacgacgaga cagcaccgat tgtccggaaa attttccagt gggcggtgga cggtcatggg
    25561 attaacttta tatccagaga actggaacgc cagcagattc cctgcccgtc atggtggcac
    25621 cggaaacggg gactccgcac ctattacaca aaatgggaaa agaaagaccc ggaaaatggg
    25681 aaatatgtat gggatgaatc ctatctgaaa tccctgctga tgaatcccgt ttactgtggt
    25741 gacatggctt cccagaaaag gtactaccgt ttcaagattg gcaatcaggg agataaagct
    25801 ccggaggact ggatcacagt cagggatacc cacgaagcaa tcattccgca ggatgtattt
    25861 gatctggtac aggcaaagat gaaaggcagg aaacgccaga cagaacaggg agaatacagc
    25921 atgtttgccg gattactccg ctgtgcagag tgtggcggtg cgttgacgtt gaagaagacg
    25981 cacaccaaag accagcatga agtttatacc tgttccactt atatccataa ggggaaagca
    26041 cattgtaccc agcaccgggt ggacgctgat gacctgtatg atgcagtcct taccagaatc
    26101 caggaatgtg caaaagcagt taccggagac ggaacagaac tggaaaaccg ggtaaaggag
    26161 ttatgcgagg aagatacaca gggacacagg gaaagtctgg agaagctggt atcaaagcag
    26221 aaagaccgcc tggaaacgtt agaccggctg attgcaaaac tgtatgatga ccttatcaat
    26281 gacaagatta cagaatctgt ttttgataag atgctggaga agacacagaa ggagcagaca
    26341 gatatcaaaa aggaactttt ccagaatgag agtgtgctga atacggaaga aaaactggat
    26401 gcacagtccc agcagtggat tgacgatatt agcgagtatg cggacatcaa agaactggat
    26461 gccaatcttc tgaaccggct gatcagtaaa atcgttatct cagaaccgca ggagaaagac
    26521 ggaacagaaa attactgccg tactcctgaa acggtcacaa tggaaattca tttcaactta
    26581 aaaccaatac cggagcttgg aaccatcgaa agaggttctg gctcccataa ataagaaaaa
    26641 tctatcaaaa tttaatctat tttaatcttt tcaaggctca tttcgagcaa atattttctc
    26701 ataaaagaca taaaacagct tgtcgctggc ggcggcgtgg ccctgatcgg tgtcactctg
    26761 gttcccctgc tgtccgggct gctgggctga tcctggcctt tgtccctgtc caaaccctta
    26821 actaatcgcc tccgcgtcct cccggccaga ccgggagggc gcgggaaagg aggtgctacc
    26881 cgtgattagt gaaatcattg aagaatggat aaaaggcatc ctcatggacg gtatcacggg
    26941 caacctgtcc gggctgttcg atacggtaaa tgccaaagtc ggagaaatcg ccgccgacgt
    27001 gggacgaacc ccgcaggcat ggaacagcgg tattttcaat atgctgcaca gcctgtcgga
    27061 aaccgtgatc gtccccatag ccggggccat actcgccctt gtcatgtgct atgagcttat
    27121 ccagatgata acggaaaaga acaatatgca cgactttgac agctccatgt tcttccgctg
    27181 gatattcaaa tccgcgtttg caatcctgat cgtaacgaac acatggaaca tcgtcatggg
    27241 cgtattcgac gccacccaaa gcgtcgtcaa tcaaagcgcc ggtgtgatta tcgggcagac
    27301 atccattgat tttaacaccc tgataccaga ccttgaaagc caattggagg ctatgagcgt
    27361 gggcgctctt ttggggctgt ggttccaaac ccttgttgtg ggcctcacca tgaatatcct
    27421 ttccatctgc attttccttg tgacctatgg ccgcatggtg gaaatctatg ttgtcacgtc
    27481 gctggggcct atcccccttg ccacaatggg caacgcggaa tggcgcggca tgggccaagg
    27541 gtatctgaaa tccctgctgg cgctgggctt ccaagccttt ttaatcatgg ttgtggtggg
    27601 tatctacgcg gtgctgatac agaatatcgg ttccgcttcc gacatatccg gcgcaatctg
    27661 gggctgtatg ggctatacgg tgctgctgtg cttctgcctg ttcaagaccg gcagtatatc
    27721 aaaggccgta ttcggggcgc attgatccca aaatccaagc atacacagga ggaacttgtt
    27781 tatttcaacg tacaacatca tttacgctga cccgccgtgg cggtatgact gcaaaaacgt
    27841 acagggcgca gcggaaaatc actattccac catgagcatt gacgagctgt gcgccctgcc
    27901 ggtggaacgg ctggcgtcca aagactgcct gctgtttttg tgggccacat tccccatgct
    27961 gccggaagct ctgcggttaa tcaaagcgtg gggcttttca ttcaagaccg tggcttttgt
    28021 atggctgaaa cagaaccgta aaagccttac atggttttac gggctgggcc gctggacaag
    28081 gggaaacgcg gaaatctgcc tgctggcaac acacggcaag ccgaagcgtc gttctgcctc
    28141 tgtccatcag tttattatca gcccgattga acaacacagc aaaaagccgg atgtgacccg
    28201 tgaaaaaatc gtagagctgg cgggcgattt gccccgcgtg gagctgtttg ccagacagaa
    28261 aacccccggc tgggacgtgt gggggaacga ggtggaatgt gaccttatcc tgacgtgaaa
    28321 ggaggtgcca aatggcttat gtaaccgtcc ccaaggactt aacccatgtg aaaagcaagg
    28381 ttgtgtttgg gcttaccaaa cggcaactga tctgtttttc cggggcgctg gtgatcggcg
    28441 tccccttata ttttctcacc cgtgactatc ttaccaacag cgcggcggcc ctgctcatgg
    28501 tatttgccat gctgccgggc tttttgcttg ccatgtacga gcgtcacggc cagccccttg
    28561 aagtggtgat tatgcagatc atccagtgct gcttcatcca gccaaaggag cgtccctatc
    28621 agaccgacaa cacctacacc gcccttgtgc ggcaatacca gatggaacag gaggtaaagg
    28681 ccattgtcca aaaaggaaat cccaagagaa accgaaaaaa caaagctgac ccgcgcccag
    28741 cgaaaagaaa ttgacgcggt aatccgtaaa tataagggcg acggcaggcc ccacacggcg
    28801 cagcagagca tcccctacga ggtcatgtac ccggacggcg tgtgccgggt atcgcccggc
    28861 gtcttttcca agtgcattga gtttgcggac atcagctatc agcttgccca gccggacacg
    28921 cagacggcta tctttgaaaa actgtgcgac ctgtataact atgtggacgc ctccattcat
    28981 atccagtttt ctttcctcaa tcggaaagtt gacccggtgc agtacgccaa gagctttgag
    29041 atcgcgccgc agggggacga cttcgacgac atccgggccg agtacaccgg cattctgcaa
    29101 aagcagcttg caaacggcaa caacggcatg gtaaagacca aatacctgac attcaccatc
    29161 gaggccgaaa gcgtgaaagc ggcgcgagcc agattgaagc gcatcggctt tgacctgtta
    29221 ggctatttca agagcatggg ggccgtagcc catgtgatgg acggctggga acggctgaac
    29281 ctgctgcatg gcgtgtacca cccggacggc gagatattca attttgactg gaagtggctg
    29341 gccccgtccg gcctctccac caaggatttt attgccccgt cctccctgtg cttcggcaat
    29401 gcaaagacat tcggcatggg cggcaaatac ggcgcggtaa gtttcctgca aatcctgtcc
    29461 ccggagctgt cggacgatat gctggccgac ttcctcaata cggaaagcgg tgttttagtc
    29521 aatctccatg tgcaggccat tgaacagacc aaggcgatca agaccatcaa gcgcaaaatt
    29581 accgaccttg acgctatgaa gattgcggag cagaaaaagg ccgtccgcag cggttacgat
    29641 atggacattc ttcccagcga ccttgccacc tacggcgagg acgccaagaa gctgctgaca
    29701 aagttgcaga cccgcaatga acggctgttc cagttgacgt tccttgtcct caacgtggcc
    29761 gacaccaagc aaaagctgaa taacgacgtg ttccaagccg caggcgtggc ccaaaagcat
    29821 aactgtcccc ttgtccggct ggactaccag caggaacagg gccttgcaag cagcctgccg
    29881 ctgggcgtca atcagatcaa gatacagcgc agccttacga cttccagcgt ggccgtgttc
    29941 gtcccctttg tgacgcagga gctttttcag ggcggcgcgg ccatgtatta cggcatcaac
    30001 gccaagtccc gcaacatgat tatgctggac aggaagcagg cccgttgccc caatgccttg
    30061 aagctcggca cccccggcag cggcaaatcc atgagctgta aatccgagat cgtcagtgtg
    30121 ttcttaacga cgcctgacga tatttttatt tcagacccgg aggccgaata ttaccccctt
    30181 gtcaaacggc tccacggaca ggtaatccgg ctgtcgccta ccagcaagga ctttgtgaac
    30241 ccgctggaca tcaacctgaa ttattcggag gacgataacc cgctggccct gaaatccgac
    30301 tttgtgctgt cgttctgtga gcttgtcatg ggcggcaaga acgggctgga agccattgaa
    30361 aagaccgtga tagaccgggc tgtgcgcgtg atctaccggc cctatctggc agacccccgc
    30421 ccggagaata tgccgatcct gtccgacctc cacaaagcgt tgttagatca gcatgtcccg
    30481 gaggctgacc gggtggcgca ggcccttgac ctgtatgtgt ccggctccct gaacgtattt
    30541 aaccaccgta ccaacgtgga catcggcaac cggcttgtgt cctttgacat taaggagctg
    30601 ggcaagcagc tcaaaaagct ggggatgctc attgtccaag accagatatg ggggcgcgtc
    30661 acggccaacc gaagccaagg aaaggccaca tggtattttg ccgacgagtt ccatttgctt
    30721 ttgaaagagg aacagacggc ggcctattct gcggagattt ggaagcggtt taggaaatgg
    30781 ggcggcattc caacaggtgc cacccagaac gtcaaagacc tgctttcctc cccggaaatt
    30841 gaaaatatcc ttgaaaacag cgacttcatt acgctgctca atcaggcgtc cggggataga
    30901 aaaatccttg cggaacgctt gaatttgtcc accgaacagc agaagtacat cgacaattcc
    30961 gaacccggcg agggcctttt gatctttgaa aacgtggtgc tgccctttac caaccccatc
    31021 ccgcacaaca cccagcttta caagattatg acgacccgct taaacgaggt ggcgggagta
    31081 tgacccggcg acgccacaaa ggaggtgaac caccatgaaa gagctgaaag ccaaggcgaa
    31141 agtgacgcag gccatgaccc gcgacgggct gacgctggaa aatcaggcca ccggcgaggt
    31201 ggagaacatt tccagccggg aggccgaaca ggatttgtcc gcgccggggc cgggcggcac
    31261 cgccgataag ctgctggaac gggccgacgc catccatgac ggccataagg ccaagaaagc
    31321 cgcgaagcag gccggggctg tggccgccga gggggacgct gccctgcacc ggccatccgc
    31381 ccggctccaa ctgtcggagg aagaacgggc cgacccggat ttacagccct atatccgaaa
    31441 atcggacaaa cgggccgata agctggataa ggcccgcgcc gccatcccca agaagcgcgt
    31501 cccggtcaag gaaaccgtct ttgacgaggc aagcggcaaa gcaaagaccg tcctgcactt
    31561 tgacaaacag gacaaggggc ctccctccct gaaacccaat ccggcaggcc gtcctgtttc
    31621 cgaggttttt ctattcggcc acgggaaaat ccatgaagtc gagcatgaaa acgtgggtgt
    31681 ggagggcggc cataaggggg aggaacttgc cgagcggcag gccggaaaag ccatccgcag
    31741 caccatccgg caccacaggc tgaaacccta ctgggacgcg gaaaaggccg agcgcaggct
    31801 attgtctgcc aacgcggagt atttctacca gaaatctttg cgggataacc cggagatcgc
    31861 acaggcggcc agcaacccca tttcccggtt ctggcagaaa cagcagatca agcgccagta
    31921 cgccaaggcc gcccggacag cgggccagac cgcaggcgcg gcggcacagg gcgcggccaa
    31981 gacagccggg gcctccacga aagcggccaa ggccgcagcc aaagaaagtg aacgggcggc
    32041 ctcctttgtg gcccgccact ggaaaggggc gctggccgtc cttgtgttgg ggcttttgat
    32101 gctgcttatc atcggcggct tacagtcctg caccgccatg ttcggcagcg ccggaagcgg
    32161 catcacggcg tcctcttact tttccgagga tagcgatatg ttggccgccg aggacgccta
    32221 cgcggagctg gaagccgagc tgcaagacta tctggacaac tacgaggcca cccatgacta
    32281 tgacgaatac cattttgacc ttgacgagat cagccatgac ccctatgtgc tgatttccat
    32341 tctgtcggcg ctccatgacg ggatatttac catcggggag gtacagggtg atttgtccat
    32401 gctctttgac aagcaatata tcctgacaga aaacgtcgtg gtagaaaccc gttaccgcac
    32461 cgaaaccgat acatggacgg acgcggaggg caacacccat acagacacct accaagtgcc
    32521 gtatgactat tacatctgct acgtcacgct ggaaaacttc gacctgtccc atgtgccagt
    32581 ctatatcatg gacgaggaaa cgctaagttt ctattccctg tatatgtcca cgctaggaaa
    32641 ccggcctgat ctgttttccg gcagccagta ccccaacgcc tccaccctga cgccgccgac
    32701 ctactacgac gtacccccgg aggctttgga ggatgccacc tttgccgcca tgctcaaaga
    32761 ggcggaaaaa tatatcggtt atccctatgt gtggggcggc agctctccca gcaccagctt
    32821 tgattgcagc ggcttcattt cgtgggtaat caatcatagt ggatggaacg tgggacggct
    32881 gggggcgcag ggcctatata acatctgcac ccctgtttcc agttcgcagg ccaagcccgg
    32941 cgaccttgta tttttcaagg gaacctacga cacccccggc gtgtcccacg tcggcctgta
    33001 tgtgggcaac aatgtgatgc tgcattgcgg gaacccgatt tcctacacga accttacttc
    33061 aaactactgg caatcccatt tttacagtta cgggcgttta ccttaacgcc agaaaggagc
    33121 tttcaattat ggcaaccaaa cttgaccgca tcgaaaagga catccagaaa accaagtcta
    33181 agatcgcgga gtttcagaaa cagcttcggg agctggaaac gcagaaaacc gaacaggaga
    33241 atttgcagat catccagctt gtgcgcggca tgaacatgaa gccggaggaa tttgccgcgt
    33301 tcttacgcag cggcgcaatg caggccgccc ctgccgcaac cccttaccat gaacaggagg
    33361 acaatgccca tgaagaataa gaccagacgg atttcccgta ttctccccgc acttctggcc
    33421 gtgatgctct gcgtcacggc ctttcccgtt accgcccttg caggcggcaa tgacccggca
    33481 ccggccccgc tgccggaggc tacaaccgaa cccgctacgg gcggtttgga gcctgaaaca
    33541 gacgaaaccg gcaccgccac ggacgggaca gaaaccgagg ccgaaaaaga taccggccct
    33601 ctgaccggca aggacatcac cgacctgttt tccgcgctgt tcggttccaa ggtgagcatt
    33661 gccgccacgg acgacggcat ccagatcacc accggcaccg acaaaaaaga acctgaacag
    33721 accggcaccg tcaccaccaa cggcggcagg ctgaatgtcc gcaccggcgc ggggcttgac
    33781 aagaccgcgt tcacccagct tcccaatggg acgacggttg aggtagtcgg cacagacggc
    33841 gactggatta aaattctctt gccggagcgc atcggctatg tccattccga ctatatgacc
    33901 gtcagcgaaa aggaagtagc ggctaccggc gagggcggtt tttccctctc cattgacccg
    33961 gaggaaattg cttctctgct ggaactgttc aatggcggcg gggccagcgc cgccctgtcc
    34021 ccggacggga acctgtcact gattgacgac atcggcagcg ccgcgaaaaa tggcaagcag
    34081 tttatcaccg tggaaaccaa aaacggcaat gtgttttatc tgattattga ccgggacgac
    34141 gagggaaaag aaactgtcca tttcttaaat caggtggatg aagccgatct gctgacgctg
    34201 atggaggacg gggaaaccgt cacccccgct gctgtctgct cctgtaccac gaaatgcaag
    34261 gccggggctg tgaacacgaa ttgcccggta tgtaaaacca acctgaccga gtgcagcggc
    34321 ccggagccgc aggaacccca gccggaggaa cccgaagccc cggaggaaga acccaagggc
    34381 ggcgcgggcg gcctgatcgt gttcctgctg gtggcgctgg cgggcggcgg tgcggccctt
    34441 tactatttca agttcaaaaa gcccaaggct gaaaccaccg gcagcgacga tttgggcgag
    34501 tatgattttg gggaggacga ggatttggag gacgaggaac ccgaaaccga gtttgacccg
    34561 gaagctgatt ttgtgagtga acaggaggac gaggaatgac atataaactt gtaatcgcgg
    34621 aaaaacccag cgtggcccgc agcattgcgg gcgtgatcgg ggccacggag aaacacgacg
    34681 gctatttgga ggggaacggc tatctggtga gctggtgcat cggacatctg gtgtcctttg
    34741 ccgacgccgg acgctatgac gagcgtttca aaaaatggcg ctatgaggat ttgcccattc
    34801 tcccggaaac atggcagtac atcgtccccg acgacaagaa acagcagttt gacgttctct
    34861 gttcgctgat gcagcgcccc gacgtgaccg gccttgtgtg cgccaccgac gccgggcgcg
    34921 agggggaatt gattttccgt ttcgtctacc agatggcggg atgccaaaag ccctttgagc
    34981 gcctttggat ttcctctatg gaggacagtg ctattaagga cggctttgcc cacctgaaac
    35041 cgggcggcga ctatgatccg ctgtatcaat cggccctgtg ccgggccaag gccgactggc
    35101 tgatcggcat taacgccacc cggctttttt ccgtcctgta ccacaagaac ctgactgtgg
    35161 gccgcgtcca gacgccgacc ttaaaaatgc tggtggacag ggacgccaag attaccgatt
    35221 tcaagaaaga gaaatatcac atcgtccata tcacggcggg cggcgcggac gcggtaagca
    35281 gccgcttttc cgacgctgcc gaggcaaaca ccgtcaaggc ggcttgtgca aaagcgcagg
    35341 ccgtttgcgt ttccgtgacg cgggagaaaa agacggaaca gccgcccaag ctctatgacc
    35401 ttaccacctt gcagcgggag gctaaccggc ttttcggcta taccgccaag cagacccttg
    35461 actatgccca ggcactgtat ctaaagaagc tgctgaccta tccgagaacg gatagtcaat
    35521 atcttaccga cgatatgctg cccgccgtgg aaagccttgt gtccggcctg tgggagctgg
    35581 tgccgtttgc caagggcctg aacctttccc cgcagtttga ccgcatcctg aacagcaaaa
    35641 aggtgtccga ccaccacgcc attatcccca ccgctgaatt tgtgaagcag ggctttggca
    35701 ctctggccga gagtgaaaaa aagctgctgt ccctgatctg ctgcaagctc ttatgtgccg
    35761 tggccgggcc gcatatctat gaggccgtca ccgccacatt tacctgtgcc gggcaggagt
    35821 ttaccgccaa gggcaagacc gtcctttccc ccggctggaa agagattgaa cgccggttcc
    35881 gttcttcctt aaagaccgac gccgacgagg acgccgaggc ggtgcgggag ctgccggagc
    35941 tttgcgaggg ccagaccttt gcagacgtgg ccgcctctgt caccgagcat ttcaccacgc
    36001 cgcccaagcc ctacaccgaa gatacgcttt tgtccgctat ggaacgggcc ggggccgagg
    36061 atatgccgga ggatgccgag cgcaagggat taggcacacc ggccacccgc gccgccattc
    36121 tggaaaagct ggtgcagatg ggctttgtcc agagaaaagg caagcagctc attcccacca
    36181 aggacggcat caatctggct gtggtgctgc cggaggcgct tacttctccc cagcttactg
    36241 ccgaatggga aaaccggctg accgagatcg ccaaggggca ggccgatcct gacgaattta
    36301 tggacggcat cgagacgcag gcgcgggagc tggtgcagac ctattcctgt atcagtgagg
    36361 aaaagcagaa attgtttcag gccgagcgtg ttcctatcgg gacgtgtccc cgctgtggtg
    36421 aggctgtcta tgagggcaag aaaaactact actgcggaaa ccgggcctgc cagttcgtca
    36481 tgtggaagaa tgaccgattt tttgaggaac gcaagaaagc gttcaccccg aagatcgccg
    36541 ctgcgctgct caaaaccggc aaagtcaagg taaagggcct ctattccgta aagaccggca
    36601 aaacctacga cgggacggtg cttttggctg ataccggggg caagtacgtc aactaccgca
    36661 ttgaacacag gagctaaagg acagtaacac agcaagcata gggagtgtgg ctacactccc
    36721 ggtaaaatcc cccggccgcg tccctgccgg gccggggatt tttgcttgtt ggggaagtcc
    36781 ccaaccccct attttttgaa acagaacaga gaaaggagtg accgcgtgaa cgaagtattt
    36841 tctatccaag tccataaccg gcagtttttg gaacagggca aacaaggcgt ctggctggat
    36901 ttgcccacca caaccgaaac gctgcaagcc gccctgcggg atgttggcat tactgccgac
    36961 aatccgcagg atttttttat caacgggtat tcctgcccgg aggacagaca ccttgccatt
    37021 ccctatgaca tggtgctggc cgccgacgtg gacgagctga atttccttgc cgcccggctg
    37081 ggccagcttg acgccgccga gatcgcagag ctgaacgccg ccctgcaaaa tccccgtggc
    37141 ggctttgaga atatcggcca gatcatcgac tacaccgaaa atgtggatta ctatgtgcat
    37201 cttcccgacg ttagggggga tggacagttg ggcgactact acctgaaccg ttccggcatg
    37261 gtggatatgc cggaggaatg gaaagccggg attgatacgg cgcagtttgg acgccacatt
    37321 gcccagcagg aacaaggcgt ttttacccag tacggctatc tggtgagaag cggcgacgaa
    37381 tggcagcgcg tccatgaggg ccagccggtg ccggaggaat accgggtgat gcactacccc
    37441 cagccggaag ctgaccggga ggaaccgcag gcccagcagc cggaggcatc cgaaagcccc
    37501 gacaaagcgc cgcccccggt tatcccgatc atgctggacg gcatgaacag caaagaacgc
    37561 atgaaagtga ttactgaccg tctggaaacc ggcgtacagg agctttttga aagtagccgc
    37621 tatatggctt acttatccgc tatgtccaag atgcacgatt acagtctgaa caatacgctg
    37681 ctgatcgcca tgcagggcgg ttcgattgtg gcgggctata acaggtggca ggatgaattt
    37741 gaccgccatg tgaagcgggg cgagaaaggc attaagatac tggccccggc cccgttcaag
    37801 gttaagcagc agatggagaa aatcgacccg gccacccagc agcccgtgat cggcgcggac
    37861 ggcaagcccg tgaccgaaga acaggaagtg acggttccgg cctttaaggt ggtgacagtc
    37921 tttgacatca gccagaccga gggcaaggat tttcccatgc cctatatcag tgaacttacc
    37981 ggcgacgtgc agcagtatca ggatttcttt gccgcgctgg aacggacatc accctatccc
    38041 ataagcattg agccgatttc agacggggcc aagggccgct gcttctacgc aaaacagcgc
    38101 attgccatca acgagggcat gagcgaattg cagaccctaa agaccgccat ccatgagatt
    38161 gcccatgcca agctccacgc cattgaccgg gaggcccccg ccgaggaaca ggcggcccgg
    38221 ccagaccgca gcacccgcga ggtacaggcc gaaagcgtgg cctataccgt ctgccagtat
    38281 tacgggctgg acacgtcgga ctattctttt ggctatgtgg ccgattggag cagcggcagg
    38341 gagttatccg agctgaaagc ctcccttgaa accatccggg ccacggccag cgaattgata
    38401 accgagatcg acggccattt tgcccagctt cagcaggagc gcgaggcccg gcaggaacag
    38461 gaggccgtca cggttgacgt taccccggag gcagcggccc cggcgcaggc cgaggaagaa
    38521 caggccccgg cccttgatcc ggcagccgag ccgcttgtga ctgtgctatg gagcgaaagc
    38581 ccccagctaa aggacggcca gcaaatgccg ttgcatgagg ccgacgcgct gtttgcccgt
    38641 ctggatgcag catatccggc agaggcaggc tatgacaaaa ccgcgttccg cattgactgt
    38701 atctttcagg gcgagccgga cagctacgag gggcggcagg attttggaga tagggacggt
    38761 tccctgatcg aacatatcca gagctaccac caattttacg cgcaggacga aaactggaaa
    38821 aagcatgtaa tcgctacaac cggcatagag ggctgggagc aggacaaggc ccatcgggat
    38881 atgctgttag gcgagtttgt cccctatatg aagctgcatt gcagccttgc ccaaatggaa
    38941 cagacggcgc agggccgctt acagtccggg gaaaccctga cgccggagga aacggcctat
    39001 ttgggggcgc ttctctctta tgtgcaggag tgccgcccca tgctcaatca gggccagtat
    39061 cagttgccgg agccgccgaa actggctgac ttcgaccaga gcttgcagga ctacaaagca
    39121 caggtacagg ccgagatcgc gcaggaggcc gccgcagccg gtatgaccgt ggaggaatac
    39181 gcggcggctg gctatgaagc accccaacag gacacctttt ccatctacca gcttcgaggc
    39241 ggggaggaaa cccgcgacta tcggtttgag ccatacgagc gtttacaggc cgcagggctg
    39301 gccgtggaca gtgccaacta tgaccttgtt tatacggcac cgctggataa aagcacgtct
    39361 ttggaggata tttaccgcac attcaacatc gaccacccgg cagattttag gggacacagc
    39421 ctttccgttt ccgacgtggt ggtgctgcac cagaatggac aggacaccgc ccactatgta
    39481 gacagcttcg gctatatgga agtgccggag tttttgcggg aaaatcccct gcgtaccgcc
    39541 gaactgtcta cggagcagaa cgaaaacatg attgacggca ttatcaacaa tacgccgccc
    39601 gaaccgcagc cggaggaacc cgaacagcta tcttttgagg atgtggcagt tgcttccggc
    39661 cagacacagg aggaaccccc ggaggctgcg cccccgcaaa cggctgtccg ctattatccc
    39721 atcaatgagg gagccgcccg ccgcgccaag gaagccatga gcttttctga ctaccggccc
    39781 ggcagcgcca cggcggaata ccggcactat gtcgatcagg ccgtggaaat tgcggagcgc
    39841 cagaaaaagc gtgtggagcc agagtatcac gaaaagatag acagcctcct tgacacctac
    39901 gcccgcaagc tggccgagaa catgaataag ggctatgaga ttaccgcccg cgtcccctct
    39961 gtcctgattg ccgggccgtc caatttcccg gtacgggcga aagaaaagca gaacgccgcc
    40021 agtgaccgca acatggagga atttcagtac attcagggct tgcttgacaa aatccgcagc
    40081 accggcatgg gtggtatcag cgccgacgat ccgcaggccg tttccaagct ggaaaagaaa
    40141 ctggaaaagc tggaagcgtc gcaggagttg atgaaggccg tcaatgccta ctaccgcaag
    40201 catgggacgc tggacggatg cccccatctg accgaaagag gcattgaaaa cctgaaagcg
    40261 gatatggcgt cgggctggca ctatgagaaa aaaccgttcc agtcctggca gctctccaac
    40321 aataacgccg agatacgccg gttaaagggc cgcattgagg aattgacccg ccaaaaggag
    40381 gcggcctatg tgggatggga gtttgacggc ggcactgtgg agatcaaccg ggtggccaac
    40441 cgcctgcaaa tcttctttga ggggaagccg gatgctgctg tacgcgacga gctgaaaagc
    40501 aacggtttcc gctggtcgcc caaggccggg gcatggcagc ggcagttaaa cgacaccaca
    40561 atccgcgtcg ctgaccgtat caagtgcatc cagccccttt ccggcgaaaa gcccagcgcc
    40621 ttacagcttg cggcccgcag ggagaaagaa aagccctcta tccgggcgca gctcaacgcg
    40681 gccaagaccg aacaggccaa gcagccaccg gcgcgggaaa aatcaaaggg attggaggta
    40741 gagtaaatgc agaagtttga aaatgtggac gttctggccg cgctggaaca gatcatgcgg
    40801 cagaacaccg cgttttatca aagtgacttt ggcattgaca aagacattat caggcgggat
    40861 gccgccagcg atcaggccgc agacaagacc ctgctctgga tgtcccggcc ctccgggacg
    40921 tactgtttcc gggaacgtga tgttttcctg aaagacaccc gacagtacaa cacatggaaa
    40981 ttttacgggg agcagacccg tgacaagata ctggcctacg cggtggagct gaccggcaca
    41041 cagggcggca ctatccgggg caatctctat gagctggact atccgcagca tttccggcac
    41101 gtcattgaaa ccgcccagcc ggtgagtgtg aaccggctct tttatgagca tggcacccgt
    41161 gacatcccgg agagccagcg ttttgacgga agccctgacc gggtattggg aaactttttg
    41221 tactatgagg cccagcccca tgacccggct gtgctgcagg aggcgctacg gacggagcag
    41281 cacagccggg ggcgggccgt ccccggcgat ttcaaagtcc acgtcaccgc tttgcatgac
    41341 agccggattg aaaccgaggc ccggcgtatt gtggacagcc tgaaagatct gtccgcgcct
    41401 aacagcccca ataaaacgca tttcatggtg gagatctccc cgcactttgc ggcgattgct
    41461 tccaccaagg ataatgaccg gctctttgcc atgctgccct ataaatccct gtgttttacc
    41521 gggatgaaag gcaggcatgg cgtgtatgcc gtgattaacc gggacgagcg ccgggatgtg
    41581 agtattcgca ggccccggcc ctctatccgc aagcagctat ccgacagcaa acaggccgcc
    41641 agcccaaaaa aggcggcggc cagaaccaaa aagaatgaat tggaggtata aggaatgatg
    41701 cactttaccg tggaggaaga aaacctgatc tgtatgtacc acaatgcaga ccggcgcaga
    41761 acgataaaca ggctcaccgc cgtcatgccg gttatggatg gggatatgcg ggcgcaggcc
    41821 cagcagaccc tttccaagct ggaacgcatg acggatgccg actatgacgg ccagaagttc
    41881 cacttcacag acgaataacg aacagcagac gccgggcgcg gcggccatgt ggtcgcttgc
    41941 gtccggcgtc ttttcttttt gcctattcct acgcatttag aacccggtat tgtcggggat
    42001 aggatgaagt attcgcttgc ccctgcaaac atgcgggaga agccttgcag cgggcggttt
    42061 tcaatccctt aaatgcgtag gttgcagcta taattagacc cctgaaacag atatagccgc
    42121 gcagcgggtg tatctgtttc aggggtgtgc aggtgatagc cgcaaagcgg cgtaagaggg
    42181 tgcagccctc ttgccgtcta tctctgttcc agcgggaagc tcggcaatgc ttccagcagt
    42241 ttcagggaaa tgcgctgccg tatatcttcg tcaatgactt tcttcttttt gccgtttgcc
    42301 tgtctgatct ccaccgtgga aagctcgttg atgtagtcag cataatgggc caacactttt
    42361 tccgtggccc acctctcacc ggcaacggcg gctttgatcg tttcatagtc cagcagcttt
    42421 tcttgtccgt ccatcctgat acccccgtca gactgatttt ttctatgata ccatattcgg
    42481 aagcatggaa ccagagaaaa cggcgctttt ctgctggtaa attctacgca tttagaacag
    42541 cacttcggcg gggataggat aaaacaccca cataccccgc tgaccatgcc ggaaaagcct
    42601 tgaaatataa gggcttttac agctctaagt gcgtaggttt ccagtatctt ttccgctggc
    42661 gctcggcctc tttgcggcgg cgcatccgtt tggagcaggc cgcgcagtat ttggcccggt
    42721 tagacttcgg cgtgaacgtg gccccgcact cacagcagcg tttcacttcg tcccggtctt
    42781 tggtgatctc ggcatacagg gccgcatcca gcgggaggac agcggcctta aaccagcggc
    42841 acagaagcga ataggaaatg ctctgtacgc aaatacattc ttcgccgtcg tccagcaaca
    42901 ggcagttgcc gtcacaatag ttgcagcact catgcgtcag cttccgggcc ttgcgatatt
    42961 gagggtaatt cattcgtgga atactcatag ctcttgcgtc cattcctttc ccggcgtcat
    43021 acgcaaaatg ccgtccacat tctgcttgat gatgccgtat tcctgcatct gctttttcgc
    43081 ttcgccgtac ttctcataca gccgttcctt ttcctcggcc aatcgcttgt actcggcttt
    43141 cagggcgtac agatcgggga gctttttcac accctgtccc tttagggcct ttgccgcagc
    43201 ttcatacaaa atcaacgtgc tttcatgctc acgccggaat tttgccttat ccgctgcatt
    43261 gcggtattcc atagccacgg gccagagctg cttgtaggta gaaatattct ttatcagcac
    43321 cgccatatcc gcaagccggt gttccatagc tttgagggta gcggcggcct cgtcgctggc
    43381 ggcggtgatc tcggccactt tgctttccaa atccgggtag gtgtctatcc catgttcagt
    43441 caggaaattc aaagtctttg cggcctgctt caaattgtgg agcttcgccc acctctcata
    43501 gcctgccgac tgctgggcct taatgttgtt ttccagatca atccgcaaag aaatcccctt
    43561 gcgctctttg ggcgctctgg tgataacgcg gcctttgata cgctctttga tggcttcctc
    43621 ggtgtaggct tcccccaatg tcttgcagcg ggtaaagcgt tcctgtccgg gagcgcggaa
    43681 agaaataaac tttcccggct tgatctcata gccctgcgcc tgcatgagcc gcaggaaatc
    43741 atcaaaatct tttgcctgcg ggatagcggc gtcaatggct gctttgagct tcgctttcca
    43801 gctcgtccct ttccgctggg cgtcccactc ggcatagctc ttgcccttac tctggccggg
    43861 cttcaccacg gacaatccgt tttccttgca cagcctgtcg ctggtgcgcc ggatataggc
    43921 atagctctgt ttgttggaaa tgtacttgcg ctgatttacc atatccacgg cacagaaaat
    43981 aatgtgattg tggacgtggc ccttatcaat atgggtagtc agcacatagg gatatttccc
    44041 ttgcagcact tcctcggcaa gctgcttccc gatctcatgg gcctgttccg gcgtggcttc
    44101 gccgggggca aaggattgta tcaggtggtg gcccagatta ttgccctttt ggtatgcctg
    44161 cgccagcagc atttcaaatt caatgtccgc tgtttcataa gagcaggcaa aggaggccac
    44221 cagcagcttg ccgtcagttt tgtccgggtt ctcgatgtag tcaagggctt ttttcagcgt
    44281 tgacttgata ggatgtatct ttgtaactgc catatctgcg ccagcgcccc ctttatatcc
    44341 tcgatgtcct gtgcgtacac cggccccata ccgttcagcc tctttgcaat ctgattgacg
    44401 ttgacgccga tcttctgtat ctcggcggtc tgcgcccgga tgtcgctggt gtccatgttg
    44461 acgatatagc cgtcaatgag catcttccgg gcgtaggctg aaaagttctt cgtatgcagc
    44521 agcgccattt tctgttcaat catagcccgt tcctccggcg tcacagggac gcgcagcacg
    44581 atttttcgct ttctgtttgc catgcttcac cgtcctttcc attcaggggt ttggggattt
    44641 ccccaacaag caaaacggac gcggcccggc agggacgggg caggccgttt ttccgggagt
    44701 ggctacactc cctatgcttg ctacgttact gtttctccac taacaagacg tttccaaagc
    44761 cccaaactac ccgcaaaata gccctctgga aagaaaaaag tgcaaaaaaa tagaagctac
    44821 gctgtgcagc tcccgctaaa agtaaaacag aggatgccag ttacacatcc tccgtttcat
    44881 tcttcttcgg cttttcaatt agtatggcgc tgtcaatcgc gccctcgatg atcggcaggt
    44941 attcttcggg gcagagtttc agcttgtggc tgaccctttg ccgcagttcg ctttcctccc
    45001 gcatgatctc cgggttaaag tagcgttcca caggaagccc gcaggcttta ataagctgta
    45061 tgacaaccgg cagactggga atggccccgt cgttttcgat gttggcaaga tagcgccagt
    45121 caatatttat tttctctgcc attgctttgc gcgaaacgcg ctttgcttgc cgggccgctt
    45181 tcacatccgc gccgaaagtt tcaaagccgg gacaatcttc aatattcgcc atatcgcacc
    45241 tcccacttac attgtatatt tcatactctg cccgtggaat gttggtatat gcacctttct
    45301 taaagtttat atttcacatt ttgtttcttt tattcttcga gcaaaagaat tataatgtac
    45361 cctgtggagg tgtactatgg actacatgac gttgaaagag gcaggcgaga aatggggtgt
    45421 gcccccacga tggataaact actattgtgc tgcgggacgc atccccggcg ctgtaaagat
    45481 ggcgacgatc tggctcattc ccaaaactgc ggaaaagccg attgacaggc gtacaaagaa
    45541 aggaaaggcg gttactgaat agtataatga aaccggcgca ttttggatat ggttttgaag
    45601 agagagaaag catggtattt tgtcacggaa aaactataag atgatagatg gcaggctgct
    45661 ccagaccgat aaaaagtatt ctcatctgaa acagaaagaa aagatttaca catggatgta
    45721 tgaagaagcg aaactttatc atgacagaaa tggaaaatgc ccgaaacaaa gacgaggaag
    45781 ctgctgctgt atcagccgta tatggtagaa ttgaaaaggc cggcatctgg attctttatg
    45841 gtgaagtctc cagacattgg ataactgctg gtctaatgcg cctttgagta tggggtgatt
    45901 gattttaata ccggggaggt acggcttttt gaggaaaata ttctgggatt tacatttggc
    45961 gattattaca tttcctgtaa ctgcaaggac acggatgatg aagatatgag gaatatagac
    46021 ttgctgattt tctactgtct tgaacggtat taggactgaa tctgctgcgc ggagtgcggc
    46081 aagagcgacc ggagggagta cacacaggcc ctgcctgtgt gaaaagccat accgcaaggt
    46141 gcattcgcaa aaaagaggcc gcgcattttg ctgaggcttg gggtgtcccc aacaagcaaa
    46201 acgagaacgc cgcaggcggg caagtggtgg atcagaatat ccgtaaagga tattctgatc
    46261 cattgcttgc cagtattatc gggatgtttt gacaaccgct gtaatcgaaa catcccaaac
    46321 attaagaagg tgttaattat tattatcttg atttaattcg tttaaaccaa taatatagat
    46381 acatacacca tgaaaagaag cgagtatccc aatgacggat gattaaaatc cgaaaagaaa
    46441 tcgctttgaa actacaggga aactacagac tgttatgtta tcttcttaaa atggagggat
    46501 ttttatgtcg atacgaattc tacttgtcga ggatgatgat catatctgta atacagtaag
    46561 ggcgtttctg gctgaggcag gatatcaggt ggatgcctgc acagatggaa atgaggcata
    46621 caccaagttt tacgaaaaca cttatcaact ggttattctt gatattatgc tgcccggtat
    46681 gaacgggcat gaacttttgc gtgaatttcg tgcgaaaaat gatactccca ttctgatgat
    46741 gacagccctg tcggatgacg aaaaccaaat ccgggcgttt gatgcagagg cagacgacta
    46801 tgtaacaaag ccattcaaga tgcagatttt actaaagcgg gtggaagccc tgctgcggcg
    46861 cagcggtgcg ctggcaaagg aaatccgtgt cggcaggctg acacttctgc cggaggattt
    46921 cacggtactt tgtgacggta cagagctgcc cctgacacga aaagaatttg aaatcctttt
    46981 gctgctggtg cagaacaaag gcagaacctt aacgcatgaa atcattttgt cacgtatatg
    47041 gggatatgac tttgagggtg atggcagcac agtccacact catatcaaaa atttgcgggc
    47101 caagctgccg gaaaatatca tcaaaaccat tcgcggtgta ggataccgat tggaggaatc
    47161 ataataatgg aaagaaaagg gattttcatt aaggtttttt cctatacgat cattgtcctg
    47221 ctactggttg tcggtgtaac agcaacactg tttgcacagc aatttgtgtc ttatttcaga
    47281 gtgatggaat tacagcaaac agtaaaatcc tatcagccat tggtggaact gattcagaat
    47341 agcgataggc ttgatattca agaggtggca gggctgtttc actacaataa ccaatccttt
    47401 gagttttata ttgaagataa agagggaagc gtactctatg ccacaccaaa tgccaataca
    47461 tcaaatagtt ttagacccga ctttctttat gtggtacata gagatgataa tatttcgatt
    47521 gttgctcaaa acaaggcagg tgtgggattg ctttatcaag ggctgacaat tcggggaatt
    47581 gttatgattg cgataatggt tgtattcagc cttttatgcg cgtatatctt tgcgcggcaa
    47641 atgacaacgc cgatcaaagc cttagcggac agtgcgaata aaatggcaaa cctgaaagat
    47701 gtaccgccgc cgctggagcg aaaggatgag cttggcgcac tggctcatga tatgcattcc
    47761 atgtatgtca ggctgaaaga aaccatcgca aggctggagg atgaaatcgc aagggaacat
    47821 gagttggagg aaacacagcg atatttcttt gcggcagctt ctcatgagct aaaaacgccc
    47881 atcgcggcta caagcgttct gttggaggga atgcttggaa atatcggtga ctacaaagat
    47941 cattctaagt atctgcgcga atgcatcaaa atgatggata ggcagggcaa aatcatttcc
    48001 gaaatactgg agcttgtcag cctgaatgat gggagaatcg tacccatagc tgaaccgttg
    48061 gacatagggc gcacggttgc cgagttgctg cccgattttc aaaccttggc agaggcaaac
    48121 aaccagcggt tcgtcacaga tattccagcc gggcaaattg tcctgtccga tccgaggctg
    48181 ctccaaaagg cactatccaa tgtcatattg aatgcagttc agaacacgcc gcagggaggc
    48241 gaggtacgga tatggagtga gcctggtgct gaaaaatgcc gcctttttgt tttgaacatg
    48301 ggcgttcaca ttgatgatac tgcgcttcca aggctgttca ccccattcta tcgcattgat
    48361 caggcgcgaa gcagaaaaag tgggcgaagc ggtttaggac ttgccatcgt acaaaaaacg
    48421 ctggatgcca tgagcctcca gtatgcgctg gaaaacacct cggatggcgt tttgttctgg
    48481 ctggatttac cgctcacatc aacattgtaa atatttaaaa cttaaatgat tttgaccgac
    48541 aggtataacc ctgccggtct ttttttgctg ctacaggaaa actacagact ggctacaggg
    48601 aaagtacaga tgcgcttgcc ataataacaa tcgtaccagc cacaaatcgt agttttattg
    48661 caaaggaggc attcgatcaa atggaaaaaa gcaactatca ttccaatgcg gatcatcaca
    48721 aacggcatat gaaacaatcc gtggaaaagc gggcttttct atgtgcgttc attatttcgt
    48781 tcacagtctg cacgctgttt ttggggtgga gactggcttc cgtattggag gcaacacaga
    48841 taccgcccat ccctgcaact catacaggca gcagcactga cgtagtggag aatttggagg
    48901 aaaacgctct tgccaccgcc aaagaacagg gagatgaaca ggaatggagc ctgattttag
    48961 tgaacaggca gaaccccatc cccgcacagt acgatgtgga gcttgagcaa ctatcaaatg
    49021 gtgagcggat agatattcgg atttctccct atcttcaaga tttgtttgat gccgcaagaa
    49081 ctgatggagt ttacccgatt gtcgcatccg gataccgaac aacagaaaaa cagcaagaaa
    49141 ttatggatga aaaaattgcc gaatataagg cgaaaggcta cacctctgca caggctaaag
    49201 cggaagcaga aacttgggtg gccgtgccgg gaacgagcga gcatcagctt ggtcttgctg
    49261 tggatatcaa tgcggacgga attcattcaa caggcaacga ggtttataga tggctggatg
    49321 aaaacagcta tcgttttggt tttattcgtc gctacccacc agacaagaca gagataaccg
    49381 gtgtgagcaa cgagccgtgg cattaccgat atgttggcat cgaagctgcc acagagatgt
    49441 acaaccaagg ggtttgcctt gaggaatatt taaaaccaga aaaatgagaa aaggatgtta
    49501 tgttatggac agaaaaagat tgacacagcg tttcccgttc ctgcttccaa tgagacgggc
    49561 gcagagaaaa atgtgctttt atgcgggaat gagatttgac ggctgtcgct atgcgcagac
    49621 gataggggaa aaatcgcttt cccatttgct ctttgaaacg gattgcgcat tatacaacca
    49681 caataccgga tttgacatga tataccagga aaacaaggtg ttcaacttaa agctggcagc
    49741 aaagacctta aacggcttat tgataagacc gggggaaacc ttttctttct ggtggctggt
    49801 acgccatgcg gacaaagata ccccctataa agacggcctt acggtgacca atggtaaact
    49861 caccaccatg tcgggcggcg gtatgtgcca gatgagcaat ttactatttt ggatgttcct
    49921 gcatacgcca ttgacaatta tccagcgcag aggtcacgaa gtaaaggagt ttccagagcc
    49981 aaacagtgac gagattaaag gggtggatgc aaccattcca gagggctgga ttgatttaaa
    50041 agtgcgaaac gataccgact gcacctacca aatatgggtg accttagatg atgagaaaat
    50101 catcggtcag gtgtccgccg acaaagagcc ccaagcatta tacaaaatta caaatggcag
    50161 tatccagtat gtccgtgaaa gtggcgggat ttatgaatat gcccaggtta aacggatgca
    50221 agttgcctta ggtaccgggg aaataataga ttgcaagctg ctttatacaa acaaatgcaa
    50281 aatctgttat cccctcccgg aaagtgtgga tattcaggag gagaaccaat gagaaaaagt
    50341 gtgggcatta ctgtttttgg atgcgaacag gatgaggcaa atgctttccg cgctttatcg
    50401 ccggattttc atattatccc tacgctgatt agcgatgcga tatcggcaga caacgcaaaa
    50461 ttggccgctg gcaatcaatg cgttagcgta ggccataagt ccgaggtttc cgaggcgaca
    50521 attcttgcgc tgagaaaggt cggggtaaaa tacatttcta cccgcagcat cggctgcgat
    50581 cacattgata cgactgccgc cgagagaatg ggaatctcgg ttggcacggt tgcgtattcg
    50641 ccggacagcg ttgcggatta tgctttgatg ctgatgctga tggccatacg gggtgcaaaa
    50701 cccaccatgc acgccgtggc gcaacaagat ttcagattgg atcgtatccg ggggaaagaa
    50761 ctgggggata tgactgtggg agttattgga accggccata tcgggcaagc ggtcgtcaaa
    50821 aggctgcggg gatttggatg ccatgtgctg gcctatgata acagccgaaa aatggatgca
    50881 gattatgtcc agcttgatga gcttctaaaa aacagcgata ttgttacgct ccatgtgccg
    50941 ctttgtgcgg atacccgcca tctgatcggt cagaagcaaa ttggagagat gaagcaaggc
    51001 gcatttttaa tcaacaccgc gcgcggagcg cttgtcgata ccggggcgct ggtggaggcg
    51061 ttggaaagcg gaaagctggg tggtgcggca ctggatgtgt tggagggcga ggatcagttt
    51121 gtttataccg attgctcgca gaaagtgctt gaccatccct ttttgtcgca gcttttaagg
    51181 atgccgaatg tgatcatcac accccatacg gcgtactaca ctgagcgtgt gctgcaggat
    51241 actacagaaa aaacaatcag gaattgtctc aattttgaaa ggagtttaca gcatgaatag
    51301 aataaaagtc gcaatcatct tcggcggttg ctcggaggaa catgatgtgt cggtaaaatc
    51361 cgcaatagaa attgctgcga acattgatac ggaaaaattc gatccgcact acatcggaat
    51421 tacaaaaaac ggtgtatgga agctatgcaa gaagccatgt acggaatggg aagccgacag
    51481 tctccccgcc atactctccc cggataggaa aacgcatggg ctgcttgtca tgaaagaaag
    51541 cgaatacgaa acacggcgta ttgatgtggc tttcccggtt ttgcatggca aattcgggga
    51601 ggatggtgcg atacaggggc tgtttgtatt gtctggtatc ccctatgtgg gctgtgatat
    51661 tcaaagctcc gcagcttgca tggacaaatc actggcctac attcttacaa aaaatgcggg
    51721 catcgccgtt cccgaatttc aaattattga taaaggtgac aagccggagg cgggtgcgct
    51781 tacctaccct gtctttgtga agccggcacg gtcaggttcg tcctttggcg taaccaaagt
    51841 aaacggtacg gaagaactta acgctgcgat agaagcggca ggacaatatg atggaaaaat
    51901 cttaattgag caagcgattt cgggctgtga ggtcgggtgt gcggtcatgg ggaacgagga
    51961 tgatttgatt gtcggcgaag tggatcaaat ccggctgagc cacggtatct tccgcatcca
    52021 tcaggaaaac gagccggaaa aaggctcaga aaatgcgatg attacagttc ccgcagacat
    52081 tccggtcgag gaacgaaatc gggtgcagga aacggcaaag aaagtatatc gggtgcttgg
    52141 atgcagaggg cttgcccgtg ttgatctttt tttgcaggag gatggcggca tcgttctaaa
    52201 tgaggtcaat accatgcccg gttttacatc gtacagccgc tacccacgta tggtggccgc
    52261 cgcaggaatc acgcttcctg cactgattga cagcctgatt acattggcgt taaagaggtg
    52321 ataagtatgg aaaatggttt tttgttttta gatgaaatgt tgcatggcgt ccgttgggat
    52381 gccaagtacg ccacatggga taacttcaca ggaaaaccgg tggatgggta tgaggtgaat
    52441 cgcatcatcg gcacaaaggc cgtggcgttt gctctgcgcg aggcacaaat ccatgcggct
    52501 gcgcttggct atggcttgct tttatgggat ggatatcggc caagaactgc ggtggactgc
    52561 ttcctgcgtt gggcagcgca accggaggac aatctcacaa aagaaaaatt ttaccccaat
    52621 atagagcgag ccgagttgat tacaaagggt tatgtggcct cacaatccag ccatagccgt
    52681 ggaagcgcaa ttgatcttac gctctaccac ctggatacag gggaacttgt ttcaatggga
    52741 agtaacttcg attttatgga cgaacggtcg catcatacag caaaagggat aggggatgca
    52801 gaggcacaaa atcgaagatg cttgcgtaaa atcatggaaa gcagcggatt tcagtcttat
    52861 cgctttgaat ggtggcacta taagttgatt gatgagccat accccgatac ctattttaat
    52921 tttgccgttt cataatgaaa gtattggttt tgtatcagat tttggatttg ataaaatcta
    52981 tgctggtcat ggacaatctt ttactggaaa gtggttaaag catcttttgt aatacattgc
    53041 aaattatagt attcaatctg cccagcggca gaaaagaaaa aaaccgctgt tgggcagacc
    53101 tttcccacgc ccattttgta ttttgctggc ggcggttttg agaaatcaag gccgccgttc
    53161 ttttctggca gcaaaaggtg tggcggccca taggaggatg ccgctatgct gttttgctat
    53221 tgccatgctc taatccactt ccaccactca aaaaa
//
LOCUS       DACDEG010000011        49819 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.011, whole genome shotgun sequence.
ACCESSION   DACDEG010000011 DACDEG010000000
VERSION     DACDEG010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.011"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1197
                     /locus_tag="IV142_000721"
     CDS             <1..1197
                     /locus_tag="IV142_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP8484398.1"
                     /translation="DLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQAIINNVDF
                     VLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERYWFSFPKNV
                     HLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYHIGLYHGEP
                     GVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGHTKKEQAST
                     SILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSKVEDGFSLI
                     EIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTFYTKTPLAA
                     SAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAKQLLNQDFL
                     FEEDQE"
     gene            1194..3914
                     /locus_tag="IV142_000722"
     CDS             1194..3914
                     /locus_tag="IV142_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8484399.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            3898..4842
                     /locus_tag="IV142_000723"
     CDS             3898..4842
                     /locus_tag="IV142_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8484400.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(4911..5921)
                     /locus_tag="IV142_000724"
     CDS             complement(4911..5921)
                     /locus_tag="IV142_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8484401.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(6101..6445)
                     /locus_tag="IV142_000725"
     CDS             complement(6101..6445)
                     /locus_tag="IV142_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP8484402.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(6438..6872)
                     /locus_tag="IV142_000726"
     CDS             complement(6438..6872)
                     /locus_tag="IV142_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP8484403.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            7010..7747
                     /locus_tag="IV142_000727"
     CDS             7010..7747
                     /locus_tag="IV142_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8484404.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            7740..8951
                     /locus_tag="IV142_000728"
     CDS             7740..8951
                     /locus_tag="IV142_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8484405.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            9158..9937
                     /locus_tag="IV142_000729"
     CDS             9158..9937
                     /locus_tag="IV142_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP8484406.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            10007..10669
                     /gene="trmB"
                     /locus_tag="IV142_000730"
     CDS             10007..10669
                     /gene="trmB"
                     /locus_tag="IV142_000730"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP8484407.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(10767..12521)
                     /gene="ade"
                     /locus_tag="IV142_000731"
     CDS             complement(10767..12521)
                     /gene="ade"
                     /locus_tag="IV142_000731"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP8484408.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            12653..13582
                     /gene="cpaF"
                     /locus_tag="IV142_000732"
     CDS             12653..13582
                     /gene="cpaF"
                     /locus_tag="IV142_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP8484409.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            13539..14588
                     /locus_tag="IV142_000733"
     CDS             13539..14588
                     /locus_tag="IV142_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP8484410.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            14585..14863
                     /locus_tag="IV142_000734"
     CDS             14585..14863
                     /locus_tag="IV142_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP8484411.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            14838..15308
                     /locus_tag="IV142_000735"
     CDS             14838..15308
                     /locus_tag="IV142_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP8484412.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            15325..15627
                     /locus_tag="IV142_000736"
     CDS             15325..15627
                     /locus_tag="IV142_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484413.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            15644..16015
                     /locus_tag="IV142_000737"
     CDS             15644..16015
                     /locus_tag="IV142_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP8484414.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            15987..16346
                     /locus_tag="IV142_000738"
     CDS             15987..16346
                     /locus_tag="IV142_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484415.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            16518..17525
                     /locus_tag="IV142_000739"
     CDS             16518..17525
                     /locus_tag="IV142_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8484416.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            17550..18734
                     /locus_tag="IV142_000740"
     CDS             17550..18734
                     /locus_tag="IV142_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP8484417.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19171..20754)
                     /locus_tag="IV142_000741"
     CDS             complement(19171..20754)
                     /locus_tag="IV142_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8484418.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(21332..21475)
                     /locus_tag="IV142_000742"
     CDS             complement(21332..21475)
                     /locus_tag="IV142_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP8484419.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(21698..22177)
                     /locus_tag="IV142_000743"
     CDS             complement(21698..22177)
                     /locus_tag="IV142_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8484420.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(22250..22534)
                     /locus_tag="IV142_000744"
     CDS             complement(22250..22534)
                     /locus_tag="IV142_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484421.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(22612..22806)
                     /gene="ssrS"
                     /locus_tag="IV142_000745"
     ncRNA           complement(22612..22806)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IV142_000745"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(22878..24164)
                     /locus_tag="IV142_000746"
     CDS             complement(22878..24164)
                     /locus_tag="IV142_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP8484422.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            24302..24973
                     /locus_tag="IV142_000747"
     CDS             24302..24973
                     /locus_tag="IV142_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP8484423.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            24939..25427
                     /locus_tag="IV142_000748"
     CDS             24939..25427
                     /locus_tag="IV142_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP8484424.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            25440..26387
                     /gene="prmA"
                     /locus_tag="IV142_000749"
     CDS             25440..26387
                     /gene="prmA"
                     /locus_tag="IV142_000749"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP8484425.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            26388..27152
                     /locus_tag="IV142_000750"
     CDS             26388..27152
                     /locus_tag="IV142_000750"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP8484426.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            27385..29598
                     /locus_tag="IV142_000751"
     CDS             27385..29598
                     /locus_tag="IV142_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP8484427.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            29609..30055
                     /locus_tag="IV142_000752"
     CDS             29609..30055
                     /locus_tag="IV142_000752"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP8484428.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            30226..31485
                     /locus_tag="IV142_000753"
     CDS             30226..31485
                     /locus_tag="IV142_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP8484429.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(31574..32521)
                     /locus_tag="IV142_000754"
     CDS             complement(31574..32521)
                     /locus_tag="IV142_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP8484430.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            32915..34207
                     /locus_tag="IV142_000755"
     CDS             32915..34207
                     /locus_tag="IV142_000755"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP8484431.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            34222..35991
                     /gene="aspS"
                     /locus_tag="IV142_000756"
     CDS             34222..35991
                     /gene="aspS"
                     /locus_tag="IV142_000756"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP8484432.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            36100..36975
                     /locus_tag="IV142_000757"
     CDS             36100..36975
                     /locus_tag="IV142_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8484433.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            37241..39124
                     /locus_tag="IV142_000758"
     CDS             37241..39124
                     /locus_tag="IV142_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP8484434.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(39199..39483)
                     /locus_tag="IV142_000759"
     CDS             complement(39199..39483)
                     /locus_tag="IV142_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP8484435.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(39473..40207)
                     /locus_tag="IV142_000760"
     CDS             complement(39473..40207)
                     /locus_tag="IV142_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP8484436.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            40336..40959
                     /locus_tag="IV142_000761"
     CDS             40336..40959
                     /locus_tag="IV142_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP8484437.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            41151..42272
                     /locus_tag="IV142_000762"
     CDS             41151..42272
                     /locus_tag="IV142_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP8484438.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            42279..45407
                     /locus_tag="IV142_000763"
     CDS             42279..45407
                     /locus_tag="IV142_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP8484439.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            45426..48620
                     /locus_tag="IV142_000764"
     CDS             45426..48620
                     /locus_tag="IV142_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8484440.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(48766..49473)
                     /locus_tag="IV142_000765"
     CDS             complement(48766..49473)
                     /locus_tag="IV142_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8484441.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16228 a         8781 c        10399 g        14411 t
ORIGIN      
        1 gatttacata tggatcggtc atttgaagga ctgacaactc ttgataaaac tgtacaggaa
       61 aaactattga agacgaatct gaccgtttta tcaaatatca ttgaacaggc aataataaat
      121 aacgttgatt ttgttttact tgcaggagat aattttcatc aaaatcgtcc ttcattaaaa
      181 atacagaaac atttttcaga acagatgaaa cggttagaaa agaaccatat acctgtattt
      241 ataatattcg gaaatcatga tttctatcaa aaagagcggt attggttctc ctttcctaaa
      301 aatgtccatt tatttacatc cgaaacggta gagacaaaaa aaataacgat aaaaagtgga
      361 gaaactgtct ctctcagtgg atttagttat cgtcaaccgt ggattcaaaa agacaaagtg
      421 atggaatttc cttctagaga actcacagat taccatattg ggttgtacca cggggaacct
      481 ggcgtctctc aaaaaggaaa ctatgctcct ttccaaccct caaagatgca agaaaaggga
      541 tacgattatt gggctttagg gcatatccat gtgccaacgg tcttaaatga aaagcaaaca
      601 attgtttatc caggtgcgcc tcaaggtcat actaaaaagg aacaagcttc tacatctatt
      661 ctactagttg aattaagtaa aggaagttgc caaatacatc cgattaaagt agcagaggtc
      721 tattggaaga caaaggaaat atcattgagg aaagcaagaa cgacaaaaga ggtcattgtt
      781 cacattcgtc agcagctttc aaaagtagag gatggattta gtttaattga gataaaatta
      841 aaagattatg accatctcaa tacagatgtt ttggaacgga tacaatcagg agagctatta
      901 gactatttgt tagaggaatt ctcagatagg ataaatgatt tctttatttg gcgtatttct
      961 ctcatagaga atacttttta tacgaagacc ccacttgctg cttctgctaa attgatggaa
     1021 cagttatttc aatactatca gacacctcaa gatttccaac aaattttaaa tgaagtatat
     1081 agtcatcaag aagcggctag aatattgagc gaactgccgg aatatcaaga agaaacactt
     1141 gaaaaagcca agcagctgct taatcaagat tttttatttg aggaggatca ggaatgaaga
     1201 ttttaaggat cgagattgct gcttttggaa aatggcgtca aaaaagtttt gacttttatt
     1261 caggcaatca gctgatctat gggggaaacg aagcaggaaa atcaacgatt tatcaattta
     1321 ttcaagcaat attatttggt ttcccttcaa aaggccgtaa aaaaaaggat tatacaccaa
     1381 aagacggctc agcctatggc ggcaaaatat ggcttaaaca tcctgtatat ggtgaatttg
     1441 ctgtcgagcg ctacaagcag caaaatcgcg gaaaatcgaa ggtgtggctg ggggatcaag
     1501 tgggaagtga tgagttacta gaaaaactga tcgcaccttt gacgaaagag ttattccagc
     1561 aggtatttac tttccagcaa gagcagttga tggaactaga tcatttgaga gaaaaagagc
     1621 tacatgatgc gctgatttct ttaggcatca cgggcagttc tcaagtattc caaaagcggc
     1681 aagaatatta tcagaaagca caacaattat tcaagaaaaa aggccaaaaa ttactaatca
     1741 atcaaaaatt aaatgaatgg aaagagctgc aagaaaaaat tcatcaaaaa caagaacagg
     1801 aagcttattt tggcaagttg atccaacaag aacaggaata cgagtcgcaa cagcagcagt
     1861 tattggcaca attaaaagag gcgaacaaac aattttcatt agccagacag caagaactta
     1921 atttttcttt atatgaagaa tggcaatctc tcaaaaaaaa cagcactaca ggtcttcctg
     1981 atgagcaact tcttttagat ctggagtctt tctccaaacg gtatcagcaa acaaatgagc
     2041 gtttggaaga attagaagca acactggaaa agcatagcgg gatggatcag cagtcagcca
     2101 gatattattt ttatttagag caggagcagg aactaaaaga tctccagcaa cttcaattta
     2161 cagcggaaca attaaacgaa gaatggcaaa gattacaaat tgagactaga gaaacaaatt
     2221 cagaattgaa gattttggaa caaaggtggc attggcagaa aaatccgtta ccggaagaat
     2281 ttttatatga agaagaatgg aagcatcttc tggctgaaga aagaaggatt gctgatctag
     2341 aaagccagat ttctgtgcgt ctccaaatag ctaaagaaca gcaagagatg attgaagcag
     2401 aagtgaatga cttggaagag acctatccag cattattaca aaatcagtca gagatagcac
     2461 cagtctcttc atctaaaccg tatatgctga tggctggcgg agcaataatc attgcaggta
     2521 tatttgtacc aatgcccttg aaagtgatcc ttttactaat tggtgcaggc atgattggct
     2581 atttcttcta caaaaggcaa ccaaaaatta aaactaaaaa tgatttctct gagataaaag
     2641 gaatgtggca agaaaaacta ggacaacttg atctagcctt agctcaatta gctgaagcag
     2701 aagaaaaaca aaagagaact ggagaagaaa aacagattct tcagcaagaa gtatatcgtt
     2761 ttgcggatca tcggcactta ggaaagatgg atacgctcaa actcatgaaa gaatatggac
     2821 aggaagtcat agattatcag aaattatatg gcgtatttga gcggaaacat aaaagagaac
     2881 aggaaataca agaaaaacta cgacaaatcg accaaaaatt tgagtttgtt aaagaatggt
     2941 taccgataca agataagaca atttcagaaa aaatggtatt gctttcccgt tttatacaag
     3001 agatggaaca gatgaaattt gccagaagct atcagcaaaa tactttattg aagcaagaaa
     3061 tcaatcaatt gaaaaagaaa caaaaagtat ggatacaaga atacagcgaa caattgactc
     3121 aagcaggctt atcttatcct tcggaagtac ctgtcttttt acaaaaagcc aaacaagccg
     3181 aacagcagat gaaaagatac aatgaattat cggaaatgct tactccacta tttcctgaag
     3241 agctgacaaa agagaaatta tctgtaagac tgcgccaact gaaagaaaag caatcccaac
     3301 tccagcaaga agaaaaagaa gcaagtgaaa atcggcaacg actccaactt caagtagaag
     3361 agttgcaaag agacggcaca ttggacgaac tttatcaaca agctagccgg caaaaagcaa
     3421 aattacagga attactggag gaatggggag cacttgaaat cgccgggaca ttcttaggtg
     3481 atctttcaac agagatgagt gaaagacaat taccccagtt gttaagagca gcaggtgatt
     3541 atttaggtat tttaacaaaa ggaaattatc agaagatatt actgaaaaat gatacactga
     3601 ctttaacaga cggatataca gaatttccca tctatgagtt atctacaggt acgaaagatc
     3661 agctgattat ggcgatgaga tttgcttacc tctcattaga agctaaccgt tcgatatgcc
     3721 cgatcatcat tgatgatggc tggcttcatt atgatcatca gcgaaagtat caattagctg
     3781 aacttttagc taaatttgga gaaaagtatc aaataatttg tttttcttct gataaagaaa
     3841 tggtaagcta ttaccatgag atgaaccaaa tcgtaaaaca attagaagga gcaaataatg
     3901 aaaaaaattc gtgatttagt aatcgatgag cagtttgaac aatttgtcct gatcaaaagt
     3961 gcagatgtaa gaatcgctaa aaacggaaaa aaatttattg cttttacttt ccaagatact
     4021 tcaggtacta tcgatggtaa atactgggat gcttcggagg aggaaatcaa taaatttaca
     4081 gccggcacag tcgtttttct aaacggtaaa agagaggttt atcaaggaaa ccctcaagta
     4141 aagattcttc atatgcgctt aacaagggta gatgagccag gagagcctag tttatacatg
     4201 gaaagagccc ctttgaagaa agaagaaatg gaagaagaaa tcaatcaatt gttgtttgag
     4261 atcaccaatg cgcattggaa tcgtattgtt cgctatctac ttaataaata tcaaaaacaa
     4321 ttttttgagt ttccggcagc aaaaagaaac catcatgcat ttgccagtgg attagcttat
     4381 catactttga caatgcttca cttagggaaa tcaatcgccg atgaatatcc tgagctgaat
     4441 cgagcattgt tgtatgctgg tatcattttg catgatttag gaaaggtgat tgaactttcc
     4501 ggtcctatgt cgacagaata tacgcttgca ggaaatttga tcggtcatct ggtattgatt
     4561 gacgaagaaa taactaaagc ctgtcttgcg ttgaaaatca gcgaagctga cgaagatgtt
     4621 ctcgtattac gtcatatggt tttagcacac catggcttat tagaatatgg ctcacctgta
     4681 cgtccaagaa taatggaagc agaaatatta catcaaatcg acaatatcga tgcttcgatg
     4741 cagatgatgt tgacttcgat tcgtcaaaca gaaccaggaa aatacaccga tcggattttc
     4801 ggtatggaca accgaagttt ctatgtacca aaagatttat agcaaaaatt tatttttttc
     4861 attcaaaaca aaaaaagcga gacataagtc ttcgcttttt ttgttttctt ttattttgat
     4921 gaatcagttg tttcttcagt tgaagattct tttgttgaat cagttgattt tgtgtcagaa
     4981 ctctttgttg aagctgtagt ttcacttgag tctttcgtac ttgatgaagt tgttgtgaat
     5041 gcactcaaca cattttcaaa ggcatcatct ttgattttta catttgcatc ttttaattct
     5101 tcaccgatta ctttagttgt gaatgcattg tcacttagtt tagtttcagt agcgatatct
     5161 tttagttgat ctttgtattt atccatgtca ttgcctttat tttggttttt caccattttg
     5221 acaacgtagt attctgtcgc ataagaagta ggatttgttg tagtaataac atcggagatc
     5281 tcaccgtctt tcaatttgaa agctgcttct tttacttctg caggaatagt tgttgtcgtt
     5341 gagtcgaatt taactttacc gccatcttct tttgtctctg tatcagttga tttgtctttg
     5401 gctaatttag agaaatcatc tccatcatcc gctgattttt tcacatcttt tgcttcatct
     5461 tcacttgata atttgatgat ttgtgcttct acttctggat ggaaagattt ccaagcagtt
     5521 ttcaaatcgt catctgtaat atctacgtga gctttcaagc cagcttcaaa agctaaattg
     5581 ttacgaatat attctttata agtatctttc gtatatccag cagcttctaa ttgtgattca
     5641 aaagtatcac ctaatgattt cgcttgtttg tcgtattctg catccacttg cttatcggtt
     5701 actttatcgc catatttgtt attgaatact ttataaatga tcatccgttg tactaaggat
     5761 tggttcgatg attcaagttt tgcttcatca tagaaatcag atacagtgat cgttccgcct
     5821 ttcatcgttg cgatatcttt attagtatcg cctgaacaag ctgctagagt taaaacagcc
     5881 aacgcactag tagctgccaa aataattgat ttttttttca ttaaaatttg cactcctgta
     5941 attagttatt atttttatat attaactata ccatatccca tttcctttaa aaacgttgtc
     6001 gtggtagttt caaacaaaat tcaaaatttc atcacattcc taggaaatac gcaaactttt
     6061 ctcacttata tagttaaatc tgttgagaaa ataatcatta ttatatttgt tcaggatact
     6121 ctggcagatc agcttttagt ttttctactt gttcattgat ttgtgtgata cgtggttcgg
     6181 cttggaattt aaaatcttcg atatcttttt tgattgcttc ttggaaagca ggaatcacag
     6241 attcagctgt ttcttttgtt tcgatcaatg cgcttttaaa atgattcagg ctgttattta
     6301 aatcatttgt cagagttgtt gcttcttcca attcttcgat cagtttcttt cttgtttcat
     6361 tcccgctcct gggcgccaat aacagacctc cgacagtgcc agcagcagct ccaaataaca
     6421 gtcctttgaa aaagttttta atcatcgcag gacacctgct ttacgattgt ttcttgaata
     6481 gcttttagtt cttccgatcc ataactatct tgatgattga caaagtgaat agaaaaatca
     6541 tcctctttgc tgtagcgagg gactaaatgg atatgtgaat gaaaaacaga ttgataggct
     6601 agttcacggt tgttattcaa gatattaaga cctttcattt ctggaaaagc tttttccaat
     6661 gcttgagcaa tcttaggcac acgcgaaaaa acctctcctg caagttcggg atcatattca
     6721 aaaatatctg ctacatgacg tttaggcaca actaatgtgt gtcctttcgt tgcttgagag
     6781 atgtctaaaa aagcatagac cttgtcgtct tcatatattt tatagcttgg gatttcttga
     6841 ttgatgattt tgcaaaaaat acagttttcc atataaaaca tcctttctat aaagaataaa
     6901 tacttcagct taactttacc ataagttaaa gctattgtaa ggaaaaaaac aaaataccgt
     6961 cattttcttc gtggtataat aaataaaaat aaatggcgga ggaaaaagaa tgagtttaat
     7021 tatcgatcat gtcacagggg ggtacggaca tctccctgtg ttaaaagata tcagtttcga
     7081 tgtacagcct ggagagatag tagggttgat tggactgaac ggagcaggga aaagtacgac
     7141 catcaagcac attatcggtc tgctggaagc ttcaaaagga acgatagaaa tcaatcatct
     7201 atccttaaag aaaaatacgg aagaatatcg caaacaaatt gggtatattc cagagactcc
     7261 ttctttatat gaagaattga cattgagaga acatatcgaa gtcactgcga tggcctacga
     7321 tatacctatg gatatagcaa tgaaaagagc agaaaagttg ttggagactt ttcggttatc
     7381 taatcgtctt gactggttcc cagctaattt ttcaaaggga atgaaacaga aagtgatgat
     7441 cttatgcgcc tttttgatcg agccttcttt atatatcatc gacgaaccat ttttaggctt
     7501 ggatccacta gctatcaatg cattgcttga attgatggta gaaatgaaaa aagaaggagc
     7561 cggtattttg atgtctacgc atattttagc tactgcagaa aaatattgtg atcgttttgt
     7621 ggtcttacat aacggagaga tacgagcaga cggttctctt gagatgttga gaaaagaatt
     7681 tcagctgccg gattcttctt tggacgaaat ctatattgct ttaacaaagg agcaggcaca
     7741 tgagtagttt ttttcaaaaa cgtttagcaa gacatcaaaa gaaaatgatg aaatatatgc
     7801 gttatgtatt gaacgatcat tttgttttag tttgtctttt cttgattggc ggtgtgggat
     7861 tatattattc taattggttg aaaaccctgc agccaccgtt tgcaattgga gggctcataa
     7921 tctctgtatt ttggatgctc tgtttattcg tgggtaagat ggctactttt gcagaggcag
     7981 cagatattgt ttttttactt cctaaagaaa aagaaatgca aacctatttg gaacgagctt
     8041 ttcgctattc ttgcattttt ccgttgatct ttttgatatt ggcatgcgga ttggccatgc
     8101 cattagtggt ggttagtacg gagcaatcct tttcgatgtt tttcgtctat ctgatcatat
     8161 tatggagttt gaaattcagt catctgcaaa tcaagagagc agaattgttt cgactagatc
     8221 aacaagttat tcgttattgg aaattaggct ggtttttttc ttctctgatc tttttgtttc
     8281 tatcatttta ttggctaata tgggcaggaa tagcaggtgc attagtacag gcaagtctat
     8341 attactggtt tttgtggaag caacaagaaa aaggattgga ttgggagaaa atggtgcatg
     8401 tagaagaaca taggttgcat cagatctatc aatttatcaa tttattcaca gatgttcctg
     8461 aaattacggc aaagatcaaa agaagacgct atttggatcc tatattgact catattaaaa
     8521 gagaatcaaa aaacacttat ctttacttgt ttgcaagaag attggcaaga ggttctgatt
     8581 ttagtggtct ttatttccga ttagtaatta taggtgggtt attgattgcc ggagtagtcg
     8641 acttttattt tagtctgatt atcggtttct tatttttata tttgatcggt ttccaactgt
     8701 tacctttagc gaaccagttc cgctatatgg ttctcgttca gctttatcca atccataaga
     8761 atcagaagaa gcaggcgatt cagtatctac tcacatgggt attaattatc gcgtcggtta
     8821 tttttggttt gattgctgca gtcgtattag atgggcagca gcgatgggtt ccgctagcgg
     8881 cttatttgat tgttactatg ctctttgtta aactatatgt accaactcgt ttgaagaaga
     8941 tgtcagactg aaaacaaagc tgtgtagggt aaaaatgaga atgaaaaata gagcgttgac
     9001 agtaagaatg ctgttcgttc tatttttttt gagaaaagct ggttttttga atggcaatta
     9061 aatgaaaaag aacgtatatg tgcttgacgt gtagtgttca ggcgactaga ataataagta
     9121 ctgcaaaaca aagagtaaga ggacgggaga atgcatgatg gagtttcagt tagaccaaga
     9181 gtggcggtta cgtcccatca aaggcgacac aggaaaaaca tacataggca tgcgtgatga
     9241 ggacaaagtc tttattaaac gaaacacgac tccgatgttg gctgctcttt caagggaagg
     9301 aattacacca aaacttgttt ggacaaaacg aacggggaat ggcgatactt tgacagcaca
     9361 agagtggctg gaaggtcgct tgctgactcc taatgagatt ggtcaaagaa acgacgtgat
     9421 tgatgttttg taccatttac atcattcaaa ttctcttaaa accatgctga cccgtatcgg
     9481 tggtcgtgtc atgacgccgg agaagatgct gcaaaaatat gaaaaagaat taccggaaga
     9541 actccagaaa aatcaatttc tgcaaattgt ccatcgtttt cttcgtgagc agatcccgtc
     9601 aatagataag gaaaacttat cagtagtaca cggagatgta aaccatcgta attggttagt
     9661 atgtaaaaat tatttatatc tagtcgattg ggattcaatc atgtttgcag accctgcggt
     9721 ggatattgca acgattttag gaaattatgt gccactgtct agctggaatc agtggctgat
     9781 cagttatggc atacgcccga ctgacgaaat gcttgaaaaa tttcattggt atgcggccat
     9841 gaatttgctc caagaaatca ccagatattg caaacgtgaa gatcatcgac ggatgaacga
     9901 agaaatttta caattaaaac gaattttcag tggctgaggt ttggcccaaa ctctgcggag
     9961 atttggccag acctttttaa ttagaggaaa ggaatggaga taaataatgc gtgtccgaaa
    10021 tcgtccggga gcagcggaaa tgttagcggc tcacccgaat tttgtaatta gtgatccaac
    10081 attgtggaag ggaaaatgga atgaattgtt tgaaaacgat catccaatcc acattgagat
    10141 tggtatgggg aaaggccagt tcatcactgg aatggcaaaa gcccatcctg aaatcaatta
    10201 tattggagta gaaatgcagg tcagtgttgt ttcgattgca ctagacaagt tgatcgaaca
    10261 accgcttcct aacctgaaac ttctacatgt ggatggatca gcgttgacag agtatttcgc
    10321 tgacagcgaa gtagatcaaa tctatctgaa tttttccgat ccatggccaa aaaaacgtca
    10381 tgaaaaacgg cgattgacat ataagacttt tcttgctgta gatgaacaga ttcttcgtcc
    10441 aaatggagaa atccatttca aaacagataa tcaaggattg tttgagtatt ctctggcaag
    10501 tttttctcaa tatggcatga tcctcaaaca ggtatggtta gatttacatc aaagtcaatt
    10561 tgaaggcaat attatgacgg agtatgaaga aaaattttct tcaaaaggtc aacgtattta
    10621 tcgagtagaa gctcaatttc aagataagac caaaaagaaa agtctgtgaa actaactttc
    10681 tgctttcata aaaacattaa tatccataaa taaatcccag aaaatgaaca ttacagatgt
    10741 aatgtatttt ctgggattta tttgatttaa aatgtattta tttcatcggt ttttccacaa
    10801 ggtggaatca attggaagcg agaaaatgaa aataatcctt tgtcggtgat cttcaattcg
    10861 ggtatgacag ataatgttaa gaaagaaagt gtcaaaaaag gatcaaaggt tttagctgct
    10921 cctagttgat gtgctttagt tattaattca ttcaactgtt catagacgat cgaaaaaggt
    10981 tcctgtgtca tgattccgcc tataggaaga gaaacacaag ctatttcctg ctgtttgttg
    11041 acaacggtca tcccgccacc tttttgaatc aaaagattag cagcatacag catatcctcg
    11101 tcgtttgtcc caacgacaac tagattatgc gagtcatggg caatcgttgt cgctatcgct
    11161 ccttgtttca gctgaaatcc tttcactatg cctaatccaa tgtttcccgt ttgatggtga
    11221 cgctcgatca ccgcaatctt taattggtct ttttcgattg aaggaatgaa tcgttcgcct
    11281 tgtcggtcca ctttttctac caaatcttta gtcactagac tattcgggat aatttcgatc
    11341 acatgggctt gtgaagaaga aagaagcagc tctaacgaat gtggatttaa tggttgagcg
    11401 tttagttttg gcagacaaca cgtatatgaa tttgaatgat cagcaaataa atggtcttgg
    11461 agttgcccag attgaacaac taatgttccc ttatagaaaa cttcatgaat tggcagcttg
    11521 gtaagatcgt ctgttaagaa gaaatcagct tgatacccag gggcaatcgc acctagtgtt
    11581 ttcaaatcaa aacattctgc tgcattcaat gttgccatct gaatggcttg taatggatcg
    11641 attccaagtg caatagcttg acggacgata tgatcgattg atccttcttt tactaaatca
    11701 ttgatcagtt tatcatcggt gacaaagaag catcttcttg aattttcagg tgtgatcgcc
    11761 ggtagtagcg atgccagatc ttttgcaacg gtcccttctc gaaccattaa atacatacca
    11821 agatccaaac gttctttagc ttcttttgca gttgtagctt catgatccgt ccgaatacct
    11881 gctgcaggat aaacatttaa ctcttccatg ccgataccag ctgcatgccc gtcaattttt
    11941 ttcttatgct ggtgcatcaa ttggatcttt tctaggatgt ctgtttcatt atttgctact
    12001 gcttgatagt tcatgacttc agctaagcct aaaacttcgg gtttttgtaa aaaaggggct
    12061 aatttttttg catttaaatt tgcaccattg gtctcaaaag gggttacagg tacacaagaa
    12121 ggtagcataa aaaagatatt catgagtgtt tgtcttgcat cttctatcat aaactcaaga
    12181 cctgtactcc cagcaatgtt tcctatttca tggggatctg tcactacagt agtaactcca
    12241 tgaagcaagg atacttttga aaattcgctt ggtgataaaa gtgagctttc gatatgcaca
    12301 tggccatcga tgaatcctgg cactagatat tgcccattag cattatgtac gctttttcct
    12361 tcgtattcac cgattcctac gatcattcct tcgcagattg ctacatcttt ttccatgatt
    12421 tctttcgtaa agacgttgac gatcttagca tgcttgatca ctaggtcagc aggctttttt
    12481 ttacttgcta cttcgatata tttccttaat gttgtttcca atggagtgcc tcccaagata
    12541 aatttttata agtataacat aaattcggtt tttgccaata tttttttgta aattcatttt
    12601 atttgaaaca aaaagatcaa aaagacgtat gttttttgtg gaggggagaa taatggaaat
    12661 aaaacgatta tcggatcaaa tgataagtga aagttttgcc aaacatgccc aagacgtata
    12721 tatattgcca gcttcagaag tattttacat ctattttcgc tatggaaaaa aacgaacatt
    12781 ctggaagaaa gtctcttcgg cagatggaca gcaactgatc tcaagattta agtatttggg
    12841 acagatggat atcggagaaa aaagaaaagc tcagttaggt gcatttactt attctttaga
    12901 aaaacaggaa gttcgtctgc ggttgtcttc tgtaggagat taccttcaac gagagagttt
    12961 agtgattcgg ctattatacg gtacttttga aacaccccat cgttgcttta gttctcgaga
    13021 ttttgcaact attttacaga aaaccgtcag acgtggatta taccttttca gcggaccagt
    13081 cggatcaggc aagacttctc taatgtatcg gttggctttg gaagaaaaat tgcaagtgat
    13141 tactatcgaa gatcctgtag agatagaaga accgtttttt ctgcagctac agatcaatcc
    13201 gaaaatagca cagacttatg atacactttt gagactcgca ttacggcatc gtccagactt
    13261 gctaatcatt ggcgagataa gagatgaatc aactgcacat gctgcggtaa gagcagcact
    13321 aacagggcac cgggtttttg caaccgtaca tgcacgcaat cttgaaggta cattggtcag
    13381 aatccatgaa ctgacaaaga aaaaagaaga gctatctgaa tgtttagctg gtgtcattta
    13441 tcaagaactt ttggaagatg ccaatcacca tccttctgtt ttatggagtt atcaatttat
    13501 ggaggaagaa tcagtaaaaa aatcatggga ggaaagttat gaagaagctc aggaaaatat
    13561 ttggcagtca gaactattct aaaggattaa caagaagcca gcaaaaccag tttgcacaaa
    13621 ccatgggaga actgctttta tctggtttta gtctgcaaga atccctttat ttattactca
    13681 cgatccgttc gtttccacct gttattttgg caaatatcca acatcagcta aaggaagggc
    13741 aagcgtttag tgaggtactt tcgcaaatag gatatggagc agagcaactg gtacagatcg
    13801 agttagctga acgtcatggg aatctgccga caactttgtt ggaaattgcc aaacagtttc
    13861 ggcttgttga aggatttcgc aatgatttga aaaagctgct tgcatatccg atgctattgc
    13921 tcaccttttt gctaggtatc ttgtttagta tgcggttgtt cgttttgcct cagcttattg
    13981 gttcaggaat ggtcatgcaa gagcattggg gtattcagct gatacaagtc tttcattggt
    14041 atgttctact attcatccta gcggttactc tattggcttt actagtaagg tatcgtctga
    14101 tgaagttgtc tcctgttgat cagagtgaat ggataagcag acaattattg attggaccga
    14161 tatattcctt gtatcaatcg gcttatttgt cactagagat tggaaagctt tttcatgaag
    14221 gtttcgaatt aaggcaaatc atagtctgct taaaggaaac gaaacaagaa agtctgattc
    14281 agcatcttgc ctctaagttg attcaaggat tggaggcagg tatgtcattg gctgagcagt
    14341 tccagaatta tcgttttcta tcagaagaat tcagtaaaat tgttttacag ggagaggcaa
    14401 agggaagttt aggtaaagaa ctgatttttt acagcgaatg gacaagaaaa cagcttttca
    14461 aacgtcttca tcactggatg cattttattc agccaatcat ttttttgggc gtagcggctt
    14521 tgattttatt agtttatgcc gctgttttat tacctgttta cggaagcata gaggaggttt
    14581 tgccatgaaa aagagaaaaa tatcttatta tagcggtttt acattgatcg aaatgctaat
    14641 cgtcttactg atcatcagtg tattagtgtt gttgttcgtc cctaatttgt cgcgttatag
    14701 gaatcatgta gaccaagaaa gtcgtgaagc gatcatccag ttggtagaca cacaaaaaga
    14761 gctctatgcg cttcaaaata atggaagagt tcctactgtg gaggaattgt tgaatgaagg
    14821 ctatatcaaa cgagaacatg cagaaatcta tcagcggcct tgaaggtaag gttttagcat
    14881 caggatatac gttgtttgaa tcactcttac tgattttatt acttacaacg atcctttctt
    14941 ttccagttct cgcattttct gcatggaaga atgaattggc agtctatcaa tttttcggcc
    15001 aatttgaaaa acgaatctat tctacgcaaa aaatcgctat tgtgaatcag atccaaacag
    15061 gcttttattg gaatagtgaa gagaatcaaa tcatttttca tgttccgaat ccagataaaa
    15121 taaattggga agtattagat attcctgatg agattacctt gaaacgccat gcttctatta
    15181 cttttgctgc tagaaccggg aatgaaagtt cactaaaagc ttatcaattt tattgggaag
    15241 aaaaagagcg gacgattacg tatcaattcc agatagggag tggtcgctat acaaaaagaa
    15301 ttgaataaaa caggatttgt tttgttggag tcccttacag cgcttatgat cgcttctgtc
    15361 tgtgtctact tagtgagctt tggccagctg tttttgcttc aggcagaaaa gaaaagtggg
    15421 gaggaactgc tatttattcg aatgatttat gaagatatca agacgcagcg gatgtatcac
    15481 aaaagaccag agcgaaggga agaaatgatc ccatacgata tccaaattgt cgataataat
    15541 ggagtaatcg aaaaagtgtt tgtcaatatg gagaaagaag cgatcagtat tgaaaaaaca
    15601 agtaattcca gcatttacgc tgattgaatg tctaattgga ctgatgatat tgagcagtct
    15661 attgctcaca ttcaatcttt tgattcagca aaccatccaa gtaagtgaat ttttgcagcg
    15721 taaagatcaa aaagagtggc taatattttt agcacaatta gaagaagaac tcaagagcag
    15781 tcacaatgtc tctgtgaaaa atcagcaact gcactacacg atcgaggata aacagtatat
    15841 catcgaaaga tatcaggcaa tgatacgtaa aagaagaact tccggcggac atcaacctat
    15901 gttaacaagt atcactgagc ttcaattatt ggaaaaagaa cacacaattt ctttttatgt
    15961 acactttgaa aatggagaag atggatatgc catatggaca aaaaacgatc agtagaaaat
    16021 gttataaggg ctctatttta ttttctgtct tgcttttatt tcttttatgt acgtttttat
    16081 tgatggcagc attagaaagc taccgtttgt caatggatct taatatacga atcaaaaatt
    16141 attatatagc aaaaataatg aagcagatgg ctttatcgga acagtctacg cttgccgaaa
    16201 gttcggaagg agttttttac tacacaactg gatctgtaac atatcagtgg gtacaacagt
    16261 ccctattctt agaggtggaa gtctcaccgt ttatctttcg gttcacagaa gaagtaaaaa
    16321 atgatacaga tactagtaca gaatgatgga atgttcgtga aaacgccctc ttttctgtta
    16381 taacactgtt tttaataaaa aaatgtttgc cgtttcacaa ataaaaaaca gtgaaaacga
    16441 ttgaaatttt gtcaattgtt cgatagaatt aaacaggtga aaaaattgtt ataatatatc
    16501 tagaaatgag gtgcaccatg ttaccagaaa aaatggaaca agggtttaat caaaacctgg
    16561 aagctattca gcttttacag aatgcattgg gaacttcctt tttagaagcc tatgtcgaaa
    16621 atgcagaaaa tttaatagat gattatcaag tacgtgtggt ggatggagtg ccgacaaaag
    16681 agacaacgca acggatcaca gctctttatg aagagttgaa gaaactttct ttcgagccag
    16741 aagaatggcg cagactgtct caattacttt tattgaaggg cagccagaca gaacatttgc
    16801 aggcgaacca tcaactcact cctgatagta tcggtttttt atttgttttt cttattgaac
    16861 aactatacac aaataaaaaa gcaccggtta agattctgga tattgcggca ggaatgggca
    16921 acttgttatt aacagttctt ttaaacctat cgaatgcggg gtaccaaaca gaaggaattg
    16981 gtgtggatat tgatgataca ttgttggctg tcgcagcttc tacaagtgat ttgacacaag
    17041 caaatgttca atactttcat caagacgggt tgcaggaatt attaatagat cctgtcgatt
    17101 ttgcaatcag cgatttgcca atcggttatt atccgaatga cgaaaaagca aaagagtttt
    17161 taacaagtac cgaagaaggc catagttatg cacatcattt attgctagag caatcaatga
    17221 aatatgtgaa gccggatggt tttggtttgt ttttgatgcc gagtggattt ttagaaacgg
    17281 atcaaagtga agaaatcaaa aaatggttta aagaagaagg ttatcttcaa ggaatgattc
    17341 aattgccaga tgaattgttc cgtaataaac aatcgcaaaa aagtattttg atcttacaga
    17401 aaaaaggacc acaagcacaa caagtaaaag aagtcttgct tgtgaaactt gcttcattaa
    17461 aagaaccaga gaaggtgacg gaatttttca acgagtttaa aaactggaaa tcatctgctc
    17521 tgtaatcatt caacgagaag gagagtcata tgtctaaaac aattgcaatc aatgctggga
    17581 gttcaagttt gaaatggcaa ttatatcaaa tgccagaaga aaccgtagtt gcgaaaggga
    17641 tcgtcgaacg tatcggttta caagattcta tttttaccat caagtacggc gataatcaaa
    17701 aattcgaaca aatcatggat atcgaaaacc acgaagtagc agtaaaaatg ctgttagatc
    17761 agttgattga actgcaaatt ttaagctctt atgatgaaat caccggagta gggcaccgtg
    17821 ttgttcacgg aggagaaact tatggggatt cagtagtgat cgatgagaaa gtaatggacc
    17881 gcattgctga attagcagaa tttgctcctt tgcataatcc tgcaaactta atggggatca
    17941 aagcgtttaa aaagatttta cctgacattt tgagtgtggc tgtattcgat acatctttcc
    18001 actcaacaat gccaaagcaa aattatttat atagcttacc ggtgtcttat tatgaagatt
    18061 tcaaagtaag aaaatatggc ttccatggca caagtcatcg ttatgtgtcg gaacgtgcgg
    18121 caaaattatt gaacaaaccg atcgaagaac taaaaatcat cacttgccac ttaggaaatg
    18181 gtgtatcgat cacagcagtc gatggaggta aatccatgga tacttctatg ggattcacac
    18241 cattagctgg ggtgacaatg ggtacacgtt caggtgatat cgatccagct gttctcccat
    18301 atttaatgga aaaattagag attgatattg atgaaatgat caatgttttg aacaagaaat
    18361 ctggtctgct aggcctaaca ggattgtcta gtgatatgcg tgatcttgaa aagaactatg
    18421 aaaaagaaca tattcgttta gcttatgaca ttttcgtgga ccgcatccga aaatacatcg
    18481 gcggttatgt gacaacgatg aatggtgttg atgcaattgt ctttactgct ggtatcggcg
    18541 agaatgacgg acatgtacgt agtgaaatca tcaaaggtat gtcttggttt ggttgtgaaa
    18601 tcgatccagc gttgaatgaa ttgcgtggtg aagaagtaga tatctctaca aaagaatcaa
    18661 aagtaagagt attagtgatc ccaacagatg aggaactaat gattgcccgt gatgtggagc
    18721 gtctgcgcgg ctaagccaaa gcattcgtta ggataaaaga tgaaacgcat gcaagaggga
    18781 attcttgcat gtgttttttt tgcggagaag accgcaaaat ggtaatttaa cttcaatttc
    18841 ccttcttttt tacaggatct tcttagggaa ttttgcttgg aaaagtggtc aaaagggaaa
    18901 agactggcat tttatttatt gattttcagg aaaatcccaa tattttattt tcattcatct
    18961 ttataaggga cattacatat gtaaaaagaa aaaacatttt tagatcagcc tcgtgggctg
    19021 tcgactttat tcttcgcttt atgtttagct agaaaaccga tccgaaacat tcattgatcg
    19081 aataataaaa acttgatttt ccacctgtac tttgcctgaa tcgtgaaagc acaggtatga
    19141 aaatcaagtt ttttgttgcg aaaaaacgtc ctagtcaatc agttggccga gcggctggcg
    19201 gctgatctgc tcctgatcaa taacgagtgc ttcttggaag tgttgctgca aaaagtagcg
    19261 gtcatggctg actgctagga gcgttccttg atagtcttgc agcatttctt caatttcttc
    19321 tcgagcatag ctgtcgaggt gattggttgg ttcatccaaa atcaggacgt tgattttttc
    19381 taaaaagagt ttcagcaacg ccaaacggat tttttcacca ccggataaat ctttgatccg
    19441 tttacccaca tcgctttgat aaaagccact tttggctaat tgccgtcgag cgacttcttc
    19501 gtttccagtc aatgtgatgg cgtaagccag caaacggcta tcgggatcgg caaactgcag
    19561 ctgctgcggc aaataaccga tttgcaagct actacctaat ttgatggtgc caccgtaagg
    19621 cttttcctca ccgagtaata atttgagtaa ggttgatttt cctgcaccat tgtcgccgat
    19681 gatggctaaa tgatctccac gatagactgc aaaactgctg tctttgaata gtacttgatc
    19741 agcgtactgc tggtgcaagt tttgaatgat gaaaacttct ttgccagatt ttccaccatt
    19801 agacaagctt tgcagccgat ttttcggggg aggtggcggt ttgacgagtg tcagtttcgc
    19861 caggcggcgt tccagttctt tggctttttt gaagaaggac tcattgtctc cttcatgtgc
    19921 ccactgacga aaacgacgaa tcatgacttt caaccgctgg acttcttttt gctgcagttc
    19981 gtaatttttt gtcagctcag ccaagcgagc ttctttcaag actcgaaaac ggctgtagtt
    20041 tccgggatac tcaatcaatg caccttcttc cagttcccat gtttttgtgg tgacttgatc
    20101 gagaaattgc cgatcatgag aaacgaccag aaccgatttt tttgtattct gtaaatagtt
    20161 ttccaaccag cgaatactgt taagatctaa atgattggtc ggttcatcga gtaaaagtaa
    20221 atcggcatcg gctgctaaaa ttttggctaa agcgacttta actcgttcgc ctccgctaag
    20281 ttctgttaac cgtgcttcct gacggtgtgg aatccctaag ccttgcaaga tcgctgcaat
    20341 ccggtcttct aatacgtaac cgcctagttc ttcaaagcga tgctgcagct caccgtaacg
    20401 ggccagacac ttctctaagt ttgtatctgg ttctgtcatc aatgcttcgt agcgactcat
    20461 ttgctgtttg aggtcctgta tctcagcggc agaatggagc aagtagtggt tgacggtcgc
    20521 ttcggaaact gtaagttctt gttcgaccgt cccgatcttt aagccttttt tgcgactgat
    20581 aacaccgctg tcgacacctt ctctgccggt caagatttgt aaaaaggttg atttgccagt
    20641 tccgttgata ccgactaagg caattttttc accagcttca atttgacagg ataaatcctc
    20701 aaataatgga ttcccgccga ttgttttttt aatagaaatt gcttgtatga tcatgatctc
    20761 tctccttgag accactcaaa aaggccatgg ttatccatgg cctgtgttat ttaaactcag
    20821 attatcatga gaaaaatacc ttgagatggc cattgatttt acgtgaattc aactgttttg
    20881 gacaaacttg tcccgtcaac agcaactgta aaaccaattg catggctaaa tgcgatatgg
    20941 tatcgttggt aattttcatt acttgttatt ttagtcatgc gcatcatagt attttccctc
    21001 cttcgtgtag ttgtcttttc cagtgtacct gaaagtgtca gaaagttcaa ctagcgctga
    21061 catttactta atatttatcg atgtgtaatt ctgtcagaga gcttttttta tctgactgag
    21121 ggtaatgtct cgtggaccac atcattgtat tctgagctaa accagcgttc aggcgaaatc
    21181 atatggtgtt tgataaaaaa ctttgtctac atgacttcct tttgccattt gcttaatacc
    21241 gttgcattaa acagctcttc tctttggaaa tctcaccaac atcgcgttca ggagtatcgt
    21301 tgtggttgca atggcaagcg gtagcctttc atcaattctt gaatgattta ccgaatgtag
    21361 cacttgcctt ctcgcggtac ccgaacccga aataaaattt tagtttggcg ataaaatcgg
    21421 tatccgccat cgatacttca tattttttcg tatcagattc atcggaaact tccacattga
    21481 cgaaccaata agctttggca tgcttaggtc gttttaaacc acaccagggc gactaatcgc
    21541 cctggtgttc actttttttt attttacttg aactcattga ctgttttccc tttattttaa
    21601 acattataaa gaccgatgag tgcttgccgc gtcttttgtt tttatcacgg ttttttgtgc
    21661 aaaaaaacct gccgatagtt ggcaggtcgt ctacgtgtta agctttggtg tggtctttag
    21721 ctggcaattg gttttctaaa tcagttcgga caacaagcac atcgcaagct gcgtttctaa
    21781 taacatattc agaaacagaa ccaataaata aacgttctac agcattcaat cctgtggctc
    21841 ctaacataat caaatctact tgattatctt ctgggatttc tctggcaata atttgttttg
    21901 gtgagccata ttctacgaca gaggttacat tattcaagcc agctttcttc gcattgcttt
    21961 cataatctgc caatgtttgc tttgccattt cagtagcttg ttctgcaagc attccatcaa
    22021 aggatgaaac ggtttggaaa gaacgtgtgt caatgacatg tgctaataga agttctccgt
    22081 tatttcggat agctacattg accgcctttt taaaagctaa ttcagcttca tcagaaccat
    22141 ctacggcaac catgattttt ttgtattgtt gtaacataag cattcactcc tcaagtcaat
    22201 cgtattgtac tcttttattg tagaacactt tagggaaaaa gagaattatt tactgctttg
    22261 gttcagcaat tttttttgca aatcggatac tgaaggtggc agaaacaata attacgatca
    22321 gtaaaaagac caaggcaata aagcgttgat tgaaaaaagc tgttaaaata cccattacgc
    22381 ctaaaacagc atatgcagct ccgtaaaatt gtaggaatcc ttgattttta tcatttttgt
    22441 tgatcaatac aaaaaagata tcctgtttat taaataaata atagctaacg aataataata
    22501 ctaaagtggc gataatcatt aaaatcctaa tcatgaacaa atcctccaaa ataagcaaca
    22561 aatagaaaaa gcaatttgcc aagagcaaat tgctttattc tttaaaagat ggtaatctcc
    22621 gttaatgccg ttgtttgtcc tgtagtattg atcccgcgaa ttcaagcagg tgggtcccat
    22681 ggtcgtagct tcgaactacc aaatgataag gcatgttacc agcagtgaca caagacgggt
    22741 cccaagatat caataaaaat gttcggtcaa cacacaaaga ggacaattcg aacatcacag
    22801 atttcccacc tctatagtag catatgaatt taaaaagagc aatgagagag ctcgcagaaa
    22861 cagaaccgtt ttcttgtcta gttttttgga ggatgctttt tccactcttt tatacggtaa
    22921 tactgttgac ccagagcctg ttcatatttc cctgtatctt ttggctgata atactgagca
    22981 ttcttcagtt tatctggtaa atattgctga ttgacccaag cctgatcaaa atgatgagga
    23041 tactgataac ccacgccacg gttgagtgat ttcgcacctt tgtaatggct atctctcaaa
    23101 tgatcaggaa catctccagc tttgccctct cgaatatcag caagtgctgc atctaatgcc
    23161 atgtaggcag aattggattt tggcgacaag caaagatcga cgacgacgtc tgctagtggg
    23221 atacgtgcct caggcaatcc caatttttca gcagctaata cagcgttcac tgtacgagcc
    23281 gcagcagcag gattacctaa acctatatct tcataaccga taaccatcag acgccggcaa
    23341 atacttgcca gatcacctgc ctcaactaaa cgggcgagat aatgaagtgc agcatccaca
    23401 tcacttccac gaatcgattt ttgaaaagcc gaaataacgt cataatgagc atctccattt
    23461 ttgtcatggg taagagcttt tctttggaca cattcttcaa tgatcgacag tgtaagatga
    23521 atccttcctt ctttatctga aggagtcgat agtgtagcca gttccagccc attcaatgca
    23581 ctccgcaaat cgccgttcgt tgcacgcgaa aggtgaagaa gagcatcttc gtctaactgg
    23641 attgcttgtt gtccaaggcc gcgttcttta tcccttaaag catgttcgac tgccaactgg
    23701 atgtcttgtt ctgttaatgg tttgacttca aagatctgtg tacggctgcg gatcgctgga
    23761 ttgatggtaa tataaggatt ctcagtagtc gctccgatca agatgattcg gccactttct
    23821 aaatgaggaa gcagaaaatc ctgttttgtt ttatctaatc ggtgtacttc atccaataag
    23881 aggatcactg taccactcat ttttgcttct tccgctacaa cttgtagatc cttttttgaa
    23941 tctgtagcgg cgtttagcat gcgaaatgca taattagtag agccggcgat cgcacttgcg
    24001 atactcgttt ttccagtacc aggaggtcca tatagaatca tggaagagag catgcgagca
    24061 tcgaccatgc ggcgtatgat ttttccttcc ccgactagat gctgctgacc aacgacttca
    24121 tcgatcgttc gaggtctcat gcgataagcc aatggctgtt gcataggatt ttcctccttc
    24181 ttgtttttac gtcccttaat tatatcatat ttagcaagat gcctagaaag aacagccttc
    24241 tgttgttttg ttttcatagc ttatccaact aatattgtgt atgataaagt aggtgagaaa
    24301 aatgacggat aaacaaaaga tagatgaatt tttttggaat aaaacgacgc cagaagtggc
    24361 acgggattta ttggggatgt atttggagca tgacacacca gaaggaagat tagctggata
    24421 tattgtagac gcggaagcat acctaggtcc agaagacgag gccgcccata gctatggttt
    24481 aagaagaaca ccgcgtgtgc gggcaatgta tgaaaaacca ggtacgatct acctttacac
    24541 gatgcatact catcggattt tgaacatcat tactcagcca gaagggattc ctcaaggagt
    24601 aatgatacgt gcaatcgaac ccgccgcaat gattgatcaa atgagtaaaa atcgtggcgg
    24661 aaaaactggt ccagatatca gcaacggtcc gggaaaatta gtggaagcat tagcaattcc
    24721 tcaagaactt tatggtcagt ctatagcaga tagttcgtta cgcttagttt ttgagaaaaa
    24781 aaagacacca aaaaagatca atgctttgcc tagaatcggt atcccaaaca aaggtgtatg
    24841 gacagaaaag ccgttgcgtt ttgttgtgtc gggaaatcct tatatctctt tacaaagaaa
    24901 aaaccaaatc gagaaagatt ggggttggag gaaagaaaat gaaaaagaag gatctattaa
    24961 catatttaga tgaaataatt gaaaaacaag tgacagacta tgatgtagct ttagattggg
    25021 atacaaaaaa tcatacgatc gagatcgttg ttcgcttgtt tgcagaaaat aaagcacaat
    25081 ttgagattga tgatgcagaa gggatcgttt cgcaagaacc aattatcgaa tttgaagacg
    25141 gcgtacttct ttttaatcct cagaaatctg tttttgatga acgcaattat ctagcagtaa
    25201 tcccttatga aggaaaaaaa gggttggcaa aatcagtagc tgattcttta gtggaatatc
    25261 taaatgaagt attagctcaa gggcaaagcg atttgcttga ttttttggat gaagacgatg
    25321 acgaagctgt attcgagctt cactgggata atcagcaact agcagaatta gtagaagtaa
    25381 aacagcaaaa tgaagcagat acttatttac cgtacccaag ttattaagga gtgacagata
    25441 tgaaatggaa tgaagtaaaa gtagaaactg aaagtgaagc agtcgaagca gttgctaata
    25501 ttttgatgga ggcaggggcg agtggtgtcg cgattgaaga ttctttagat gttgaacatt
    25561 ttcagtcgga tcaatatggt gagattttag ataaaaatga ttttacatcg ctgaaagaag
    25621 gcgcactagt catggcttat tttccggaaa cgactttctt accggaaatc ttgcctttta
    25681 tcaaagatcg tatcacacaa ttacctgagt ttggtctagc tattggaaaa aataaagtaa
    25741 cagttagtga agtagaggaa agcaactggg caacagcctg gaagaaatac tatcatccgg
    25801 tgcgtattac gcgaatgctg acggttgcgc caagctggga aaactaccag acagaagatc
    25861 ctctagaaaa aattatcaca ctagatcctg gtatggcatt tggcacaggc actcatccca
    25921 cgacttcctt gacattgaca gcactagaaa cagtcttgcg tggtggtgaa acattgctag
    25981 atgtgggaac tgggtcgggc gtgctaagta ttgcagcaaa atatcttggt gcaaaagatg
    26041 tctatgcctt tgatttagac gaagtagccg ttcgttcggc gaaagaaaat atggatatga
    26101 atgaaatagc caaagatgtc catgtatcag ctaacgatct tttgacagga atcgagattg
    26161 aaagtgatgt aatcgttgcg aacattttag cagatatcat cttattgatg atcccagatg
    26221 cttggcgttt actgaagcaa acaggcacgc tgatcgtttc aggaatcatt gaagagaaga
    26281 agcaaatggt tctggatgcc atgttagaac aagggtttat cgtggatcaa attttccagc
    26341 aaaaagactg gtatgcaatt attttgaaaa aaccagaggt ggaatagatg cagcggtatt
    26401 ttttaaaaga atcatatgaa tcaaaagact attttgtttt agaaggggaa aactatcacc
    26461 acattgtacg ggttatgagg atggaacccg aggatgaagt ttttttggca ttcaatgatc
    26521 aaatagccat tgttgcagaa atcacggaga ttactgaaga ggctgtttat ctgaaagaaa
    26581 ttaaaaaaga agaaaatcaa aaggaactgc caagttcgat taccatagcg agtggctatc
    26641 ctaaagggga aaagctggat tggatcgttc agaaatccac tgaattagga gcgcaagctt
    26701 ttcttggttt cccggcatca gcctctgtcg ttaaatggga tgcaaaaaaa cgtatgaaac
    26761 gacaacaacg gcttgaaaag attgctcaag aggcagcgga acaatcccat cggcagatcg
    26821 tgccatcagt agagttattg gaaacgaaac aagatttatt tgagcggcta gatgattatg
    26881 acgtggtctt ggttgcttat gaggaatcag caaagcaagg cgaaaaatca aatgcagcca
    26941 aaattcttag tgaaacaaaa ccagatagtc gaatcttagc tgtctttgga ccagaaggtg
    27001 gattatcccg cgatgaagtc gattattttg tagaacacaa agggatactt tgtggattag
    27061 gaccaagaat ccttcgtaca gaaacagcac ctctttattt attaagtgct gctagctatc
    27121 aatgggaatt aatgaatcct tctttcagct aacgttcttc attaataaaa gagtatcgac
    27181 agaaagaatt gaaaaaatag tcaacaattt gtataataga aacaattatt tatacaatgg
    27241 atacgaaaaa tcgtttgaaa aagcttttaa aaagcgaaca tttccgtaaa aaaacgtata
    27301 actgcacgga ttttgggaca attatcaaac agatatttgt cccgattccc gttttaccga
    27361 aaaaagaaag ggggatcgat tcctgtgaca aaagaagaaa ttttaacggg acccggtgtc
    27421 ataaagctcg ttagtcaata catgggtccc gaacatgtag catttgttga gaaagcatgc
    27481 gaatacgcga ctgctgcaca tgacggacag tttagaaaat caggcgaacc ctacattatt
    27541 catcctatcc aagtcgcagg tatattagca gatttaaaaa tggatcccca tacagtggct
    27601 acaggcttct tacatgatgt tgttgaagat acagaaatca ctttagaaga tctgagagaa
    27661 gaatttggcg atgacgttgc tatgttagtg gacggcgtaa ccaaattagg gaaaataaaa
    27721 tataaatccc acgaagagca gctagcagaa aaccaccgaa agatgctgct agcaatggct
    27781 caagatttac gagttatcat ggtcaaatta gctgatagac tacataacat gcgtacgttg
    27841 aagcacctgc gagaagataa gcaaagaagg atcgctcagg aaactttaga aatctatgcg
    27901 cctcttgcac atcgtctagg gatcagccgg atcaaatggg aattagaaga tacagcactt
    27961 cgttatctaa atccaaaaca gtattaccgt atcgtccact tgatgcagac gaagagagaa
    28021 gaacgtgaaa aatacgtaag tggtactgtt gaagatattc gaatagcgac agaagagctg
    28081 gggatttttg cagaaatcta tggacggcca aaacacattt attcaatcta tcgtaaaatg
    28141 aaagatcaga aaaagcagtt caacgaaatt tatgacttgc tagctattcg agtgatcgta
    28201 gattcaatca aggattgtta tgctgtatta ggagcaatcc acacaaaatg gaaaccaatg
    28261 cccggcagat ttaaggatta tattgccatg ccaaaagcta atatgtacca atctttgcat
    28321 actaccgtga tcggtccggc aggaaatccg gtagaaattc aaatccgaac acaggaaatg
    28381 catgaaatcg ctgaattcgg ggttgctgca cactgggcct ataaagaagg aaaaaatgaa
    28441 aaagtagaac cagatggtat gacgaaacaa ttaagctggt tccatgagat actcgaactt
    28501 caagacgaaa gctatgatgc ttctgaattt atggaaggcg taaaaggaga tatctttagt
    28561 gataaagtct acgtcttcac accaaaagga gacgttactg agttaccaaa aggatccgga
    28621 ccattggact ttgcatacag tatccataca gatatcggta acaaaaccac tggtgcaaaa
    28681 gtaaatggca aaatggtgca gcttgattac aaattgaaaa acggagatat cattgagatc
    28741 atgacttctc caaattcatt tggcccaagt cgcgactggt tgaaattagt tgctactagc
    28801 aaagcaagaa ataagatcaa acgtttcttc aaagcccaag atcgagaaga aaatgtgatc
    28861 aaaggccacg aatccgtggt caaatgtatt acagatctag gatttacgcc taaagatatt
    28921 ttgacgaaga acaaactgca agaagcactc gatcgtttta attatcaaac agaagatgat
    28981 ctctatgcag ctgtagggta tggagaagtt agccccttga cgatggccaa tcgtctgact
    29041 gaaaaagaac gtaaagaaca aaaaatcgag cagcaaaagc aagaagcaga agaaatcatg
    29101 aatcagccga aaaaagaacc tgacaaaatg aaagtacgtc atgaaggtgg cgttgtcatt
    29161 caaggggtag aaaacttact aattcgtatc agtcgctgct gtaatccgat tcctggtgat
    29221 gatatcgttg gttatatcac taaaggcaga gggatatcca ttcatcgtcg agattgtccg
    29281 aatgttcagc ctgacaaacc aaatgtagca gaacgtttga ttgaagtcga atgggaagat
    29341 acatcgaata cacgaaaaga gtatgatgca gatttggaaa tttacggcta taatcgttca
    29401 ggcttattga atgatgtact tcaaacagtc aatgcgctaa cgaaaaatct caacagcgtt
    29461 gaagcacgga cgaataaaga taaaatggcg acgatccatt tgacggttgg tatccagaat
    29521 ttatcccacc taaagagtat cgtggataaa atcaaagcag tacctgatgt ctacagtgta
    29581 cgccggacga atggatagga ggacagatat gagagcagtt gttcaacggg taagtaaagc
    29641 tgctgtgtca gtcgatcagc aagaaattgg aaaaattggc tacggcctac ttgttttatt
    29701 aggcattcat gaaaccgata cgcaaaaaga tgtggactat ctggtaaaaa aaattgcaca
    29761 aatgcgtatt tttgaagatg aacaaggaaa aatgaatatt agtgtagaag atgtgggagg
    29821 acaaatttta agtatctctc aattcacact gtttgcggat acgaaaaaag gaaatcgacc
    29881 aagctttgtg gaagccgcac gaccagaaac agctattcct ttatacgaat cattcaatga
    29941 aggaatcaga agtcgcggca tcacagtcgc taccggacaa ttcggagcag atatgtcggt
    30001 ttctctcgtc aatgatggac ctgtaacgat cattattgat tctaaaaaca aataaagtaa
    30061 tctggttttc actagtaaaa actatgaaac acgtcgctta agttagactg ataaactcta
    30121 acttaagtgg cttttttgtc tgcaaaaagc gcttcttttg tcagcagtcg gaaaaaaagt
    30181 taagcttaaa ttatacaagt tattttttat aggaggttgc tgaagatgaa agaagtacca
    30241 gtagtagata tcaaaatcac agatttaaaa aaattgtatc aaaaaggata caacatgttg
    30301 gaggagttac gccatgaagc ggacgctccc gtcgtcaaag caaagatttt caataaagaa
    30361 gctatcacga tctacggatc atcagcagca aaagtatttt atgatccaag gaatttcaaa
    30421 cgaaaaggtg ctatgccaaa attagtattg aagacacttt ttggtcaagg tggagtacag
    30481 actttggatg gagctgctca tcaccatcgg aaaaatattt ttatggatct aatgacaccg
    30541 gaaagaatgg aagattatca tcgtatctta gataagaatc tgacacaagc acttgaagca
    30601 cagcatggtc aatttgaact atttgattta agtaagatgg ttttctttac atctatttgc
    30661 gagtgggcag gtatcaattt atcagcgatt tctaaagatg aggtcgaaaa attagcagaa
    30721 tatcaaatct ctatgatcag cggaacgttt acttctccga ttgatcacat aaaaggtgtc
    30781 gaaaatcgca aaaaatcaga aaaatgggcg caaggcttga ttgaagaagc tcgacaaaat
    30841 cctgtggctg gaaaagaaaa tgtggcatta tatgcttttg ccaatgccac agatttagat
    30901 ggtcagcttt tacctctgga agtggcagct gttgaattat tgaacatcat ccgtccaacg
    30961 gttgctctaa ctgtgtgggc ggcattgatg ggacatgcac ttttcagtag accagatttg
    31021 tatcagcaat taaaaaatga cttttcgacg ttacaagatc cgtttattca agaaatgaga
    31081 cgctattatc ctttcttccc aatgctacct gcgatttcgc tgaaagaagt agaagtagat
    31141 ggttatcgta taccagaagg cagttgggtt attttagatt tatatggaac agaccatgat
    31201 gaacgaacag ttgaagcgcc agattccttt atgatcaaac ggtatgtagg gaaagcaaaa
    31261 gatatttcct ataaagaaga atatgagatg attgcacaag gcgggggaaa ttttcgacaa
    31321 atgcatcgct gtgcaggaga atggatcact cttcatagtc tacgtgtgtt ttccgatcag
    31381 ctagtaaata agtttgagtt ctctgtacct gaacaagatt ggacgattcc attcaatcag
    31441 tttccgactt atccgaatag ccgagcgcta ctttacaaga attagattag cataaactta
    31501 tcgaaaaaaa ggaagatcaa acagtgctga tcttcctttt ttcatattac tataaattga
    31561 taaggtcaat tgatcaaaat ccacgaaatg cttcatcaat ggtttgataa tcttcattgg
    31621 ataaatgcaa atctaaagct tggacattgt cagatacttg ctctggacgc ttagctccag
    31681 gtataactac agacactcgt ggattcttca tgtaccaagc taaaacaagt tgcgcaggag
    31741 tggcttggta tcttttagca atggggcgaa gtacatcgac agcggttaaa atttctctga
    31801 aacgttctcc ttggaaatct ggatttcttt ttcgaggatc tccttcacca aattgttttt
    31861 cctcaccaag ttcatatttt cctgtcagca aaccggaagc aagaggaaaa tagggaacaa
    31921 aactgatttt gtttttttca agataaggga ataattcttt ttcagcctgc cgatggatca
    31981 aactgtattt atcttctact acatcgacat atccatctgc attcgcttct ttcaattgtt
    32041 ctaacgtgaa gtttgacaca ccgactgctc ggatttttcc tgcttctttt aattcgtgca
    32101 atgtagcaac tgattcattt tttggtgtgc tttcatccgg aaagtggata taaaaaatgt
    32161 cgatgtaatc agtttgcaga cgttttagtg catcttccac cgattgtttg agaaactcag
    32221 gagaattatc aaatgttcgc cctttatttg gtacatgagc agcttttgta gcgatgacga
    32281 cacgtgagcg atcatattct ttttccttca gtacctcgcc aatcagttct tcggaacggc
    32341 cgtttccgta agcataagcg gtatcgatca aattgatccc gctattcaaa gctgtacgaa
    32401 ctacttgttt tcctgtctca tcttccaaac cgtcaaaaag attatgtcct ccaacggcat
    32461 ttgctcctaa tccaagctgt tctgcgtaga cttgtgaatg tccgattctt atttctgcca
    32521 tagctgattc ctcctttttg tctagtctaa aacaaaatac caaaaagctg aactaaaacg
    32581 tttttgtgag gggaaatagt cagatatctc ttgactttga gactggtttt tagtaggtta
    32641 taagtaatat cgaatgtcaa tgaaagagcg agtaagtttt tgaaacttta caagagagga
    32701 tcatcgaggc tgaaaatgat ctaaagtttc attagcctaa agacactctg gagatggacg
    32761 agtgaaaagc cgtcccgcgt acgagcgtta ttcgtaagag gaaagagaaa tctttcaaaa
    32821 ttaggtggta ccgtgtatga atttcattca cccttgttca tgatgaacaa gggtgatttt
    32881 tttatttaca tagctaagaa agggagagaa aactatgagt ttccaaagac caaaaggaac
    32941 aaatgatatt ttgccaggcg aatcagaaaa atggcagttt gtcgaagaaa cagcacgttt
    33001 attattcaga gattatcagt ataatgaaat gcgtacaccg atttttgaac actatgaagt
    33061 gatatcccga agtgtagggg atacaacgga tatcgtttca aaagagatgt atgattttta
    33121 tgacaaaggg gaacgccatg tgacgcttcg accagaaggt acagcaccaa tcgttcgttc
    33181 ttttgttgag cataaattgt tcggcccaga gtatgcgaaa ccgttcaaaa ctttttatat
    33241 gggaccaatg tttcgctacg aacgccctca aaaaggacgg ttacgtcaat tccatcaaat
    33301 cggagtagaa gcatttggat cagaaaaccc tgcaactgat gtggagagca tggtgatggc
    33361 acttgatttc ttcaaacagt tggggatcac gcaaatccgg ctagtcatca attcattagg
    33421 tgataaagaa acgcgtcaag cctatcgtca agcattgatc gactatcttt taccttttga
    33481 atcagagctg agtgaagact ctaaacgccg ccttcatgaa aatccattac gcgtattgga
    33541 cagtaaagac aaacgagatc aaaaatttgt ggcagaagct ccatctatct tagattatct
    33601 aagcgagcct gctcaaaaac attttgatac cgttgtttcc atgctggatg cattggctgt
    33661 tccttatgaa atcgacagta atatggttcg cggactagat tattacacac atacgatttt
    33721 tgaaatcatg agtgacgcac caaaaatggg ggcacaagca acgatttgcg caggcggacg
    33781 ttatgacaat ctagtggaag aactaggtgg tccttcaaca cctggcttcg gatttgcaat
    33841 gggtatcgag cgattactct tgactatgga ggcagaggaa gtagttatcc ctgcatttaa
    33901 tgagttggat gcttacgtgg tcgcactcgg agacgaaaca aatatcgaag cattgaaagt
    33961 ggtccaagcg attcgaaact ttggtttttc agctgaccgc gacttcatga accgtaaagc
    34021 aaaagcgcaa tttaagacag cagataaagc gaatgccaaa ttagtattgg tgatcggtga
    34081 agatgagctt gctagtcaaa cgattaatgt caaagcaatg gcaagccgaa aagaaaaaag
    34141 ttttccatta gaagagatat atgaacattt tgatgaagtc tatgatgaaa tgactttatt
    34201 cgagtaggag gaaaaaaata aatggcacaa agaacgatct attgcggatt agtttccgaa
    34261 caacaattag aacaaacagt tacattaaaa ggatgggtac aaaaaagacg agaccttggg
    34321 ggagtaatct ttatcgacct acgtgatcgt gaaggaatcg ttcaaatcgt ctttaatcct
    34381 gaaatcaaca aagaagcatg ggaaatcgcg gataaatgtc gcagtgaata cgtgatcgaa
    34441 gtgactgggg aagttagaag acgcggagaa aatgcagtaa atcctaaaat ggccactggt
    34501 gaattcgaag tgattgcaag tgaaatcgtg attttgaatc aagcaaaaac cccgccattt
    34561 caaattgaag atgatcaagc aatcagtgac gaaatacgtt tgaaatatcg ttatttggat
    34621 ctgcgtcgtc caaaaatgac taaaaacatc aaattaagaa atgcgacaac aaaagcgatt
    34681 cgtcattatt tagatgataa tgactttctc gatatcgaaa caccttacct agggaaatct
    34741 acaccagaag gcgcaagaga ctacctagtt ccttcacgtg tccatgctgg acatttctat
    34801 gcgttgccgc aatctcctca attgtttaaa caattgctga tgaatgctgg attcgaccgt
    34861 tattatcaaa tcgttcgttg tttccgcgat gaagatttac gtggagaccg tcagcctgaa
    34921 tttacgcaag tagatattga aacaacgttc ttaacagcag aagaaatcca aacttacaca
    34981 gagggattga ttgcgaaggt aatgaaagaa gttcgcggta tcgaagtcac attgccattt
    35041 cctcgtatga cctatgatga agcaatggct cgctacggca gcgacaaacc ggatacacgt
    35101 tttgatatgg aattgatcga tctaagcgat acagtcaaag aagtagaatt caaagtcttc
    35161 caaatggctc ttgaaaacgg cggtgtcgtg aaagcattga atgctaaagg cgctgcagat
    35221 cgctactcac gaaaagacat ggatcagtta ggtcaatatg taggacaatt cggagctaag
    35281 gggcttgcat ggctgaaagt agaagaagac ggattgaaag gtccaattgc taaattcatg
    35341 ggagaagcaa cagaagcaat cattaaagca acagatgcaa aaccaggtga cttgctgatg
    35401 tttggcgcag ataaaagtga aatcgtcgct gctgcattag gtgctatccg gacacgttta
    35461 ggaaaagaat taggactaat tgatgaatcg aaattcaatt tcttatgggt aactgattgg
    35521 ccacaatttg aattttcaga agaagaaggt cgatatgtct ctgctcacca tccattcacg
    35581 atgccaaaag aagaagatat cccgttattg gctgaagacc ctgcaaaagt ctatgcagaa
    35641 gcttatgata tcgtcttgaa cggttatgaa ctaggcggag gttctatccg tattcatact
    35701 cgtgagcttc aagaaaaagt actagaaact ctaggcttta caaaagaaca aatggaagaa
    35761 caatttggtt tcttgttaac agctttagat tacggattcc ctccacatgg cggtattgca
    35821 ttaggattgg accgttttgc aatgttatta gccggagaag ataatattcg tgaagtcatt
    35881 gcctttccta aaaacggaaa agcagcagat gtgatgagtg atgcaccaag tatggtttcg
    35941 cctttacaat tgttcgaatt gaatattgat gtaacgaata tagaagaata gccgataaaa
    36001 aggtaaaaag gatttgtcga atacgcttct tttgacaacc ttgatttaat tgaaaaacac
    36061 tttataatga agccataaca tataaagcgg ggcttttcca tgaaaaaaac attcgaaaat
    36121 tgggcttatc atgattacac gaccaagctt tcggtatcta tcgtttatgc gttgatcgca
    36181 tcagtcgcat tgaatttctt ttatcagcca ggacatattt attcaagtgg tattactggt
    36241 ctagctcaga ttttgacgac tttgtcaaaa tcactgatag gattcgaagt accggtatca
    36301 acaactttgt atttactgaa tatacctttg tttgtattgg cgtggttaaa aatcgggaag
    36361 aagtttacaa ttttcacttt tatcacagtc actttgacct ccctttgtat ccatatttta
    36421 ccggaaacag tcctatctac cgatccaatc atctgtgcgt tgtttggggg ggctgtgatg
    36481 ggactaggta ttgggctagc gttgagaaac ggcctatcat cgggcggtct agatatcgtg
    36541 agtatcacga ttcgtaaaaa aacaggaaag tcgattggtt ctatttcgat ctacttcaat
    36601 gcattgatcg tttttgttgc cggaattcta tttggctggc aatatatgtt ttacagtacg
    36661 ttgtctattt tcgtcagtgg aaaagtaact gatgtcgttt atactaaaca aaagaagatg
    36721 caggtaatga tcgtaacgaa acatccagaa aaagtagtaa atgaaatcca aaactgtatg
    36781 cgtagaggga tcacgatcat caatgaagca gaaggtgctt ataagcatga taagcagaca
    36841 gtattgatga cgatcgttac acgatacgaa ctgcctctac ttagagaagc aatgaaagct
    36901 tctgatccac aagcttttgt aagtattgca gaaaacgtcc agatacttgg tcgtttctat
    36961 gaggatggca tttagatcga gtttgacaaa aattcatgaa atactgctgt agacagacgt
    37021 tcgtttgtct gcagtttttt tctaccatag tagaatcagc aaagaaaaac tgctttttta
    37081 tttgaaattt tcttaaagtt gaaaaaaagc atgtttattt ttttaaattt atgggatgat
    37141 agaaaaaaat agtagagaaa ggacacgatt ctacttgcta ttttcactta atacccgtat
    37201 attacgaatg aaaaaagttt atgaaaagtt ggaatattga atggaaaaac aaaaaaggtt
    37261 aaaaaacagc cgctacatca ctgggtttga tgggctccgt tcattagctg tggtgggtgt
    37321 tatcttctat caccttctgc caactagtat gaaaggcggc tatttgggcg ttccgatttt
    37381 tttcgtcgta tctggttatt tgatcacgga tctgctgcgg caggaatggg aacaaaatgg
    37441 aaaaatcaat atttggcaat tttatgtcag aagaatgaag agactctatc cggcacttgt
    37501 ttttatgctt gtgacagctt cggcttatat cactttgttt cagcgagggc tgttaaacaa
    37561 tcttcgagga gttgtggtca gcagcttact gtatgtcaac aactggtggc aaatcaataa
    37621 cggtttatct tatttcgatc gatttgctaa tgagtcaccg tttacgcata tctggtcttt
    37681 agcagtagaa ggtcaaaact atttggtttg gccgatcttg tttgtattgt tgatggtatt
    37741 tgttcgtaaa agaaaatgga tcttttatac tgtgttaggt acatccttcc tctccgccat
    37801 cttgatgatg atcttgtata caccaggagg cgatccaacc cgtgtttatt acggtaccga
    37861 cactcgtctg ttttctatat ggttgggaag tgcgcttgcg tttgtttggc caagtacgcg
    37921 tttaaagaaa aacatcccga aacaagcgaa aaatattctg aatatcgctg gaggaatctc
    37981 gcttttggca ctgattcttt gcttcttctt tttagatgat cattacaatt tcatctatta
    38041 cggaggaatg ctcctagtga gtctgttatg cgtagtgcta gttgcagtga cagcacaccc
    38101 cggagcaagt ttgaatcgat ggctgacaaa tccagtattt acttggattg gcaaacgaag
    38161 ctatggcatt tatctgtatc agtttccagt tatgattttt tatgaagcaa aggtgactga
    38221 tattgccgat catgttttgt tgcatacttt agtggaaatc acattgattc taatcatcag
    38281 tgaactttct tatcgatatg tggagcgtcc gctagcacgt tttgattatg gtcgtacatt
    38341 ccaagtgatg aaaggatggt tcagcaaacc gatcatcagt aagagcaaac catgggttgt
    38401 tccaggcaca ttacttgtag caattgcact cgttggtttc gttacggcac cgaaaaacgc
    38461 tgtaacagcc gatcaaaaac aactgcaggc aaaaatcgct gaaagtaaaa aattagcaga
    38521 agaaaccaga aacaattcag aaaacaccac tagttcggtt gaccaagcga tccttgataa
    38581 gtacgagttg tccaaggcac aagggaataa ggcacaaaaa ttagaactaa cggcatttgg
    38641 tgattcagtt atgttggatg ctgcaacgga tctacaagaa ctttatccaa aagtagtggt
    38701 agatggagat gtcggacgac aattatatgc cagtgtaccg tacattgaaa aattaaagga
    38761 acaagatttg ttgaaagaca ctgttctgat cggactagga acgaatggct cattctcgga
    38821 agctcagttt gatagtgtga tggatgcaat cggtgatcga aaagtatact gggtcaatgt
    38881 ccgagtaccg acgaagcgct ggcaaaatga cgtgaatgcg atgcttgaac agatgacgaa
    38941 aaaatacgac aatatgacgc tgattgattg gtacagctac agtaatgacc atagtgactg
    39001 gttttatgat gatcaagtcc atccaaatcc tgaagggatg acacattaca ttcacttagt
    39061 cagcgaagcc ttgctaggag gaagttcaag tacgaacagc agtgagacaa acgtgacaga
    39121 atgatagcta caaaaaactt caggcaaaaa catttgtttt tgcctgaagt ttttttatat
    39181 ttaccgtgtt tttaacattc atatagattc aggaagcaaa acggttgagt gtgtctgtaa
    39241 ataacgttct ttttgttttc gggtgagttt tttgaatgca gcttctgctt ttgttgcttc
    39301 gcttcttgaa gaaaatactt ctgcatggat catcttaaca ggtcttcttt tttgaagacg
    39361 agtatattta gcaccagtac cattgttatg ttcctctaat cgtctagaaa gatcggttgt
    39421 atatccacca taaaatgtct gatctttaca gaataatacg taaaaataat gttcatttgc
    39481 catagagcat ctcacgaatc tctgcagtgt attctccatc catattgtaa gtgatcaatg
    39541 gtggagcgac tttgaatccg tctaatttgc cttctttgat tccttcaact aacaaaatat
    39601 ttgcctcttt tccaggtttt gggtaaacaa attggatcct tttaggtgca attcgatagt
    39661 cttccatgac ctgaatgatt tctaagaacc gatcaggacg atggaccata gtaaagcgtc
    39721 catttgtttt tagtagctta cttgaaatat cgatcacttc ttctaacgtc gtatgaatct
    39781 catgacgggc gattgccagg tgtttattag ggttcgtttt atttgttggc agccctttga
    39841 aataaggtgg attacagacg actaagtcac aagaattatg tcgaacgaca gataagctgt
    39901 ctttcagatc gatggtgtgg acagtcattt gttcatttaa atggttaagc gcgatactac
    39961 gtcttgccat gtctgctaga cgaggttgaa gttcgatagc gtcgatagcc gcctgggttc
    40021 gtttacttaa aaaaagtcca actgcgccat ttcctgcaca aaggtcaaca atcttccctt
    40081 ttttaggtac acgggaaaag tgagcaagca gcacagcatc taaagaaaaa gaaaacactt
    40141 tggaactttg gataatcttg atatcttctg cgtataattg gtcgattcgt tcgtcttcgt
    40201 gaagcatgga ttccctccaa aatgaaaaaa atagtcgtct gtattatact atttttatta
    40261 aaaagaacca aggtggcttg tcaaacctct attttttatg catactatga ccataagaag
    40321 ggaaaggaag agactatgtt ttatcgattt atgcgcgggg tagttcgagt actattagcc
    40381 ttagttaatg gaaatgcccg ctatgagaat aaagaagttt taccgcaaga tgagaattac
    40441 atactagttg ctccccatcg tacttggtgg gacccattat atctggcggt agctgcagca
    40501 cccaaggaat tcagctttat ggcaaaagaa gaactgttca aaaatccagt tctgcgattt
    40561 atattaactc gatccaatgc ttttccagtt aaaagagaaa atccaggacc aagcgtcatt
    40621 aaaacacctg taaagatttt gaaatcgaca aatttgagtt tgatcatgtt tcccagtggg
    40681 acaagacatg ctacagaact taaaggcgga atggcattga tttccagaat gtctaaagcg
    40741 aaaatcgtgc cagctgtata tcaaggtccg ttgacacttg gggatctgtt caagcgtcag
    40801 agagtgacag tgcgtttcgg cgaaccaatc gacttatcag atatcaagaa aacggataaa
    40861 gaaggtatgg cggagatcga acgacgaaca caagcagctt ttgacaaatt ggacaaagaa
    40921 gtgaaccctg atttcaaata tgaagtcaaa aaaaaataaa atgataagtt tatgcgtggg
    40981 cggtcttttg aatctactgt cctgttcagt tcagactttt tcaatgaaaa cgagggagag
    41041 acgtcaagtc tgccctcttt ttgcgttcca atatgtaaaa tagaagacag gtgtcatgta
    41101 atggatcaaa gtgttataat aattagaagg acattttttg aggtgattga atgcgttttt
    41161 tacatacagc cgattggcat atcggaaaaa aactacaagg ttatgacttg attgaagacc
    41221 agcgtcatgt actatcttct atactagata tcgcgaaaga agaaaaagta gaggcaatcg
    41281 tgattgccgg agacttatat gataggagtg tcccagctgt tgatgccgta gaattataca
    41341 atgaattgat ggtcgattgg aatttaaagg aaaagatccc gatttttgct atatcaggaa
    41401 atcacgatag cagtacgaga ctttcagcgg gtacgccatg gttcagttat tcgaactatt
    41461 ttttgcgaac acagctagca gaagcatttg aaccagtcga gtttaaaaac gtacaatttt
    41521 tctttcttcc ttactttgaa ccaattgcag cgagattata ttttgaagat gacaatatcc
    41581 gaacgatcaa gcaagcggta gccaaggtga tagataaaat gcaggagcta tttgattcag
    41641 aaaaaaaaca agtattgatt agccattttt ttgtaatggg aagtgtaaaa tctgattcgg
    41701 aaacgaaaat cgaagtaggc gggttggatt cgatccctgg tgaactactt acaccgtttg
    41761 attatgtagc actaggacat ttgcataatc aagctgcttt gagacaggat aatgctcgct
    41821 atagcggttc cccattgaaa ttctctttat ctgagatcaa ccaaaaaaaa ggcgtatgga
    41881 tcgttgacct gacaacaggc ctggagttga attttaggga gatcgaaccc ctgcatgata
    41941 ttgaagaaat aacagcgagt ttcaaagaat tgctggatcc tgccttttat cagtccgtca
    42001 atcgggagaa ttacatctat atctatctca ctgatagaac gatcatcccg aatatgatga
    42061 accagcttcg gcaaatatat cctcgtattt tggggattga acgggcaaac ggtcgagcag
    42121 aacaaacaaa gcgcaaacga aaagtactag aaatgaaaga tcctcaaaaa ttagcagaag
    42181 cattttttag tgaaatgacg gaagagcagc taactgcaaa ccaagaaaaa tggctcggag
    42241 aaacgttgca agaaatcatg gaaatggaat gatcaagaat gaaaccgaaa aaattacaac
    42301 ttaaaaattt tggtcctttt atcaatgaga ccgtagattt ttcacgtttg acagaagcac
    42361 cgcttttttt gatcagcggg aagacaggag ctggcaaaac gacgattttt gatgggatca
    42421 ccttcgcact ttttggtgaa acttccggaa gacttcgctc tggaaaagag atgcgttctt
    42481 tgtttgctac accagacgaa gaaacaagcg tgactttttc ttttgaacat cagcagatga
    42541 gctatgaaat cgaacgaaaa ccagaacaag ttttagcaaa acgaaaagga aacggaacaa
    42601 aaaaacaggc agccaaagta acgttgacca tttttgatgg gaaaggaaaa gaaataaaac
    42661 aaatcacaaa acgtacagaa gcagatcagt tgatcagaga actgatgaat ctcgatgcga
    42721 aacagttttc tcaaatcgtt ttactacccc aaggagaatt ccgtaatttt ttggtttcat
    42781 caagcagtga taaagagact gttttacgtc atttattcgg tactcaattt ttccaacagt
    42841 tcaatgagcg attaaaagaa aaagcaaaac aacagcaaca aaaattggat catttagagc
    42901 aagagctgca tcttctgcaa aaaaggtttg ttttagtcaa agaagaggaa ccagcagcag
    42961 ggtttgaagc cgttttagcc caatggacaa accatcaggc aatactcgaa aaacaaatca
    43021 cggaagaaaa agaagctcta acggaacttc aggaaaaaca aaagcaacta gaaaattcct
    43081 actatcgttt tgaaagcttg aaaaaaagtt atgaagaaaa aacgatatta ataaacaagc
    43141 aaaaggaatt agctgctcaa aaagacgaaa tagaggaaaa gaaacgatgg attgggtatt
    43201 atgaatttac gcaacgatta gtcgaaccaa tccagcgcct acaagaaata aaagttgaaa
    43261 aaatagaact gtcacaaaaa agtagtgagc aaacaaagaa actagcgatg actacgcaga
    43321 attatgaaga gtggcaaaaa aatgaagcaa accgcgtacg ccgacaaaca aaaatcaaag
    43381 aagctgctca aaaactgcaa aacaaacaga tgatggtgcc aattgtcgaa tccttacgta
    43441 gtaaaaaaca agacgcagaa caattaacaa tttcattgga taaagaagaa ttgaatgccg
    43501 agaagctgta tcaaaatctc gctatctatc aggaaagaac tcgaattctt caagaaaatt
    43561 tactggatca ggaaaaactg caagaagatc gtcttgcttt tggacagtta aaatatctag
    43621 atgaaaagca gcaagaaata gaaaaacaaa aagcaaaaga cactgggtcg ctcgcacaga
    43681 taagagaaga actcaaaaaa gcaactgaag aaataacaaa aaatagtcag ctattgaaag
    43741 aacagacaga acaagcagaa cagctgaaaa gccaatgggc aaagcagcaa attgctcgtc
    43801 ttcagctttt tttgcgtcca ggggagccat gtcttgtttg tggatcaacg gaacacccca
    43861 tacaacaatt aaaacacgaa cagccaaata aggaagatat cttaaaagta gaacaacaac
    43921 tggcagaagc tgagtctgct gtagaaagaa ctcagcaaat cattgccaaa agtgaagcac
    43981 atgaaaataa tcttcaacaa caattatcag atttagaaca aaaaatcatg gcaacgaata
    44041 tacaattggc tgaacaagaa gaacacttgc aacaggcttt gaaaaaggca tttcctgaga
    44101 taacagaaca aaaaacaaaa acaactcttg tagtaatcga acaatcttta gatagacgaa
    44161 aaagcaagat ggccaatgca agaatagagc tggaagaaat aagacaacaa gcagaaaagc
    44221 tcgaacaaca attagctgtc tacaaacagc agcagacaga aaaaaataat cagctccagc
    44281 aactgcaagg agaaatcaaa agtttagaac aacaattgca agatacggat agtaactgtt
    44341 tagaagagga gatacgacga cttgaaaaac agttagaaga agactcgaat caactagctg
    44401 ttgagcagaa agcaggagtg caattgttgc aagatcttca tacgcttcgg acacagcttg
    44461 atcttcaaca tgaacaaata gaaaaaatag atgaaaaaat agaaaaggaa caagaaagaa
    44521 tagctaaaaa gttagaaagc cagtcgtatt tttcaacaat tgaagaaatc tgtgcatttg
    44581 cacaacagca agcacaatat aaagaaaacc aacgtacagt tacttcttat catgaagagc
    44641 aattagtgac agctgaccgg ctgcgtcaat tagaaaatat ccagctaact gagacattcc
    44701 cagatacaga agagatcagc caacagttag aagaaataag acgtatgatc gaaataaagc
    44761 aagaaaagat atacggttta caagaacaaa aaattagtaa tcaaaaaatc tacgatgaat
    44821 gtctttcgat ttatcacaca agccagacga aattagatga gcttacgcaa ttacagcagc
    44881 tttcgcaaac attcaacgga gaaaatccta agaagactag tttggaacgg tatgtgcttc
    44941 aggtttatct tcaagaagtg ttacaagtag caaatgatca tctccaacgt ttaacgaaaa
    45001 atcgttatca atttgagcta gcggatacca ttggcagtta tcgagggaaa accggattag
    45061 aaatcaatat ctatgatgat caggcaggta tgtctcgaag tgcgcatacc ctgtctggcg
    45121 gtgagagttt tattgcagca ctttctttag cattagctct tgccgatgtg atccagacac
    45181 aagctggtgg tgtagcaatc gatgcattgt ttattgatga aggatttggg tcattagatg
    45241 aagaagcttt ggaaatggca atggaagctt tagaaacgat cgaaagtgaa ggaagaatga
    45301 tcggcatcat tagtcatgta agagaattga aagaacgagt attacaacaa ataagaattg
    45361 atacagaagg tagcggacaa agcaaaatac gctatcaact tggataaaaa gggagattgg
    45421 taactatgaa atggagtatt ccagaaaaag tcattgcacg tggacgtcaa tacttgcaag
    45481 aaggccgagt gttatcggtt gtccctgacc aagaagaaaa cgtgtggcat gcagaagtct
    45541 tgggcagtga attgtatatg gttgatttgg atggtacagc aaaagagata gattactgcc
    45601 aatgtcccta ttgggaggaa cataaatatt gtaagcatac agttgctgtg gaactttatt
    45661 tacggtcgaa aaatctatct cgtatcatga aaaaagacca gccaatgcta gaaccggtaa
    45721 aagcaacaag tgagggagaa atcattgcaa aaggtttttc tcgtctaaaa acacaaaaca
    45781 atcctcaagc ggtgcaacct ttgattattg aataccaagt agaaacgatt gagactaatc
    45841 agtatcaccc agagctttct attttggcac tttacttgcg tatcggttca ttggcatcac
    45901 cgaaaaaaac atatatcgta aaaaacatct atgatttttt acaagcttac acagaaaaag
    45961 aaacttacac tgttaataaa cagtaccagt ttcgtctaga taaacgggcg tttcaaaaag
    46021 aagatcaaaa aatactcacg catcttgcag gaagtgctca gacgcatcaa ttattaggta
    46081 cgaacggctt acaagtcaaa ggaaaactag ataaaagata tctgcttctc ccagtagaac
    46141 aagcccgctt cctgttagag cagatgaatc agactacacg tttgcatctg cagataaatg
    46201 aacaaaacga tcaaggaata caattttccg atcaaaacaa acctttatca ttcaaagtgg
    46261 aaaaatttgg agaagattat ttgctgcaag cattgaatga ttttgatttt ttctttatgc
    46321 attatcagtg gggagttatc caagggacat tttattctct gacgaaatat caacaaacgg
    46381 tttatcaaac ttggaaacaa ttgattcgcc gattggagaa accagaagta gtgttcaaga
    46441 aaaaagaatt accgtctttg tttaaagaga ttatccctct gctgtcagag atcggcgacg
    46501 tagctattgc ccctgcagta gaagaggaag tagcagatat ccccgtcgaa tttattttct
    46561 ttttccgaaa agcaaaagga aaaatccaag ctcgagtcga tttcgtctat ggcgaagtca
    46621 tctattcgac tgacccgaag catgaggtct cgacagaaca ggcttttcag gtgttacgtg
    46681 acacagtaga agagaaacga gtacttgatc tattcagaat gtatcgctac cagaaaaatg
    46741 aaactggtta tgaacgtcca ttgccaagcg gggaagaact atatgccttt ttccgcacag
    46801 aactatctgt cttccgtcag tttggtgaag tcagacttgg aagaaaattg cgagagttgt
    46861 atttagatgc gcataaacat caaccgaaaa tcgaagtaac cgagaccggt tcatggctcg
    46921 atattcgatt cgatgtgaca gggatagagg agcaagaaat cgatcatgtc ctgcaaagct
    46981 tgctgagaaa tgatgctttt tatacattag aaaatggtca ggttctttct tttgattcag
    47041 aagaatttca acaaacaagc caagtgttgc aacaattacg ggaatcgata cgtacagaag
    47101 aaggaacgat ccatgtacct aaaaatcaag gattgatcat tcaaaatcaa ttggaaaaaa
    47161 gcaatgctac ttttagtgaa tcgttccaaa cgatggtaca agatctcatt catccagaaa
    47221 gataccaggc acagcttccc aaaggattga atgcaacgat gagagattat caaaagcaag
    47281 gttttcgatg gttgaagatg ttaggtcatt atcagtttgg cggtatttta gccgatgaaa
    47341 tgggacttgg gaaaacacta cagacaattg cctttttact ttcggaaaaa gaagaacgga
    47401 aaagcttctc tgctttgatc gtagcgcctg cgagcttaat ttacaactgg caggcagagg
    47461 tgagaaaatt cgccccttct ttaagtatcc aagtaatcaa tgggaataaa aaagaacgtg
    47521 aagaattact ggctaaagac acagatatcc gagtgacttc ttatgccagc ttaagacagg
    47581 atctagctaa ttatcagtcg caaaaaattg attatttgat tcttgatgag gcacaaatgg
    47641 tcaaaaatag cagtacgaaa actgcacaag cattgagaga attagctgta cctcagcggt
    47701 ttgcactgag tgggacacca atcgagaata atttagaaga attgtggtca ttgtttgcaa
    47761 cgattatgcc aggatttttc ccgactaaaa caaaattcag ggaacttgcc gcagaagaaa
    47821 ttgcgcaaat gattcgaccg tttattttac ggcgagataa acaaactgtg ttgaaagact
    47881 tgccggaaaa aacagagatg aatctatatt ctgcacttac agaagaacaa aaaactgttt
    47941 atttagctta cttgcgtcaa atgcgtgaag aaatcacatc aatggattct gaagcattca
    48001 agaaaaaccg gatcgggata cttgctggtc tgacaagact gcgacaaatt tgctgcgatc
    48061 ctcgtctgtt tattgaagat taccaaggcg gttccggcaa gctcgaacaa gtaaaagatc
    48121 tactggtagc tgccaaagag aacaaacgaa gagttttgct gttttctcaa tttacaggga
    48181 tgctgactat cttgcaagaa gagttagccg agctagggat ctcgacattt tatttaagag
    48241 gcagtacgaa accacaagat cggttaagta tggtagacgc atttaatgct ggcgaaaaag
    48301 atgtttttct gatttcctta aaggctgggg gaactggatt gaatctcacg ggagcagata
    48361 cggtcatctt gtatgacctt tggtggaatc ctgccattga agaacaagct gctggacgtg
    48421 cacatcggat cggacaaaaa aatgtggtag aagtgtggcg tatgatctct gaaggaacga
    48481 ttgaggaacg catggattca ctccagcaag aaaaacgcga actgttccaa aaagtgatcc
    48541 aaggtaatga agaacagttg acgaaactga ctgaagaaga catccgcatg attttaagtg
    48601 taggagaaat cgaggagtaa gagactcgaa aaagcgttca gtaatgggta agaaagcctt
    48661 tctaaagaag cgattgttta tttgatttaa aaaatagcaa ggaggctggg attccatttt
    48721 atcccagctt ccttgctatc atttttttat ttctattttt aaaggttata caaagattgt
    48781 cttcattgtc tctaacagct gatcaaaaga agaaatctcg atatcgcaaa ttggagtctg
    48841 ttcaattttg cgttctcgat ggttgaacca aagcgcttgc cagttagcac tttttgcacc
    48901 tagaacatca ttgtcgtaat tatcgccaac gtataaggtc tcttctggaa gcatatgaaa
    48961 ttctttctca gctagctgaa aaatctcttt ttctggtttt tggaaacctg ttgcttgaga
    49021 aatgagcata ttgcgggaag gaatccaatt gttcaattga agttgcttca gtttttttgt
    49081 ctgatggtca gtcggcccat ttgtgataat tcctaatggg acattctttt ccttcaagaa
    49141 atcgagggtt tttttgacct cctcatgcaa gcagatatta tcaagttctt cctcgtaaat
    49201 tttttgaaat aagaggccat cttcttccgt aatatgaggg tagtctaaat cttttagtga
    49261 ttggctgatc cgatgtgctc gcatatattc tagtgtccaa tctcctgcca ttacttttgg
    49321 gaagttttca tcactatgat ggcggaaccg gatatatagt ggatgcatat ctgcatctga
    49381 aacaagcgga acgactcttt tgaccgcgtt tcgaaatggc tgctgctgat catacatcgt
    49441 atcatcaaca tcaaacacaa ctgatttaat caaaatttta cacgcctttc ggttttcatt
    49501 gattttttca caaatccttg actattgtaa cagaaaaaat tgtctaaaaa agctgataaa
    49561 taaaggtttt tcttactata tttctatttc cttgacagaa taaggttttt atatttcagt
    49621 ttttttcact aaaaatcgtg aaacacgtca ataagtttga tgaacaaatt aatcattatg
    49681 gtagaagtcg attgatttat tttaatgcaa tctctgtgga attttcgaaa gatttatttt
    49741 tttaatacag tctcaatata actattcaaa cataaaataa cgttatttga tttatttgtt
    49801 tatagtaaat tttatttag
//
LOCUS       DACDEG010000012        49502 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.012, whole genome shotgun sequence.
ACCESSION   DACDEG010000012 DACDEG010000000
VERSION     DACDEG010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.012"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            273..593
                     /locus_tag="IV142_000766"
     CDS             273..593
                     /locus_tag="IV142_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP8484442.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            747..1652
                     /locus_tag="IV142_000767"
     CDS             747..1652
                     /locus_tag="IV142_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8484443.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1649..2422
                     /locus_tag="IV142_000768"
     CDS             1649..2422
                     /locus_tag="IV142_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8484444.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2603..4027)
                     /locus_tag="IV142_000769"
     CDS             complement(2603..4027)
                     /locus_tag="IV142_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8484445.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4238..4684
                     /gene="nrdI"
                     /locus_tag="IV142_000770"
     CDS             4238..4684
                     /gene="nrdI"
                     /locus_tag="IV142_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP8484446.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(4749..6731)
                     /locus_tag="IV142_000771"
     CDS             complement(4749..6731)
                     /locus_tag="IV142_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8484447.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6745..7641)
                     /gene="murQ"
                     /locus_tag="IV142_000772"
     CDS             complement(6745..7641)
                     /gene="murQ"
                     /locus_tag="IV142_000772"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP8484448.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(7644..8705)
                     /locus_tag="IV142_000773"
     CDS             complement(7644..8705)
                     /locus_tag="IV142_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP8484449.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(8916..9443)
                     /locus_tag="IV142_000774"
     CDS             complement(8916..9443)
                     /locus_tag="IV142_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8484450.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(9510..11012)
                     /locus_tag="IV142_000775"
     CDS             complement(9510..11012)
                     /locus_tag="IV142_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP8484451.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(11270..12391)
                     /locus_tag="IV142_000776"
     CDS             complement(11270..12391)
                     /locus_tag="IV142_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8484452.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            12517..13458
                     /locus_tag="IV142_000777"
     CDS             12517..13458
                     /locus_tag="IV142_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP8484453.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13608..14504)
                     /locus_tag="IV142_000778"
     CDS             complement(13608..14504)
                     /locus_tag="IV142_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP8484454.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            14773..16113
                     /locus_tag="IV142_000779"
     CDS             14773..16113
                     /locus_tag="IV142_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8484455.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16331..16774
                     /locus_tag="IV142_000780"
     CDS             16331..16774
                     /locus_tag="IV142_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP8484456.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17278..18696)
                     /gene="nhaC"
                     /locus_tag="IV142_000781"
     CDS             complement(17278..18696)
                     /gene="nhaC"
                     /locus_tag="IV142_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP8484457.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(19099..20322)
                     /locus_tag="IV142_000782"
     CDS             complement(19099..20322)
                     /locus_tag="IV142_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP8484458.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20353..21192)
                     /locus_tag="IV142_000783"
     CDS             complement(20353..21192)
                     /locus_tag="IV142_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP8484459.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(21209..21889)
                     /locus_tag="IV142_000784"
     CDS             complement(21209..21889)
                     /locus_tag="IV142_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8484460.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(21891..22964)
                     /locus_tag="IV142_000785"
     CDS             complement(21891..22964)
                     /locus_tag="IV142_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8484461.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(23611..24282)
                     /locus_tag="IV142_000786"
     CDS             complement(23611..24282)
                     /locus_tag="IV142_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8484462.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(24369..27047)
                     /gene="mgtA"
                     /locus_tag="IV142_000787"
     CDS             complement(24369..27047)
                     /gene="mgtA"
                     /locus_tag="IV142_000787"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP8484463.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTRFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            27706..28143
                     /gene="ndk"
                     /locus_tag="IV142_000788"
     CDS             27706..28143
                     /gene="ndk"
                     /locus_tag="IV142_000788"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP8484464.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(28165..29031)
                     /locus_tag="IV142_000789"
     CDS             complement(28165..29031)
                     /locus_tag="IV142_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8484465.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(29191..31284)
                     /locus_tag="IV142_000790"
     CDS             complement(29191..31284)
                     /locus_tag="IV142_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8484466.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(32033..32656)
                     /locus_tag="IV142_000791"
     CDS             complement(32033..32656)
                     /locus_tag="IV142_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484467.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(32815..34212)
                     /locus_tag="IV142_000792"
     CDS             complement(32815..34212)
                     /locus_tag="IV142_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8484468.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(34244..35215)
                     /locus_tag="IV142_000793"
     CDS             complement(34244..35215)
                     /locus_tag="IV142_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8484469.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            35589..36014
                     /locus_tag="IV142_000794"
     CDS             35589..36014
                     /locus_tag="IV142_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484470.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(36502..37203)
                     /locus_tag="IV142_000795"
     CDS             complement(36502..37203)
                     /locus_tag="IV142_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8484471.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(37219..37659)
                     /locus_tag="IV142_000796"
     CDS             complement(37219..37659)
                     /locus_tag="IV142_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP8484472.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(37659..38234)
                     /locus_tag="IV142_000797"
     CDS             complement(37659..38234)
                     /locus_tag="IV142_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP8484473.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(39082..39561)
                     /locus_tag="IV142_000798"
                     /pseudo
     CDS             complement(39082..39561)
                     /locus_tag="IV142_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(39720..40421)
                     /locus_tag="IV142_000799"
     CDS             complement(39720..40421)
                     /locus_tag="IV142_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8484474.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(40529..41938)
                     /locus_tag="IV142_000800"
     CDS             complement(40529..41938)
                     /locus_tag="IV142_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAP8484475.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(41986..43851)
                     /locus_tag="IV142_000801"
     CDS             complement(41986..43851)
                     /locus_tag="IV142_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8484476.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(43972..44823)
                     /locus_tag="IV142_000802"
     CDS             complement(43972..44823)
                     /locus_tag="IV142_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8484477.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(45133..45846)
                     /locus_tag="IV142_000803"
     CDS             complement(45133..45846)
                     /locus_tag="IV142_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP8484478.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(45914..46240)
                     /locus_tag="IV142_000804"
     CDS             complement(45914..46240)
                     /locus_tag="IV142_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP8484479.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(46472..47689)
                     /locus_tag="IV142_000805"
     CDS             complement(46472..47689)
                     /locus_tag="IV142_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8484480.1"
                     /translation="MSEKRRDNRGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     gene            complement(47771..47974)
                     /locus_tag="IV142_000806"
     CDS             complement(47771..47974)
                     /locus_tag="IV142_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP8484481.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            complement(48435..48665)
                     /locus_tag="IV142_000807"
     CDS             complement(48435..48665)
                     /locus_tag="IV142_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8484482.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            complement(48662..49084)
                     /locus_tag="IV142_000808"
     CDS             complement(48662..49084)
                     /locus_tag="IV142_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8484483.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
BASE COUNT        14201 a        10416 c         8523 g        16362 t
ORIGIN      
        1 gtttttttta aatgattaat aaataacaac ttctttctat gaaattgcaa agcattcatc
       61 ttcttttccg ctaacgatat atccgtcagt gcttggtaca ctttactaaa aagtatgaga
      121 ttgccgatat ctggataggt gtgctatact acagaaaaag atatacaatc tgccaccggg
      181 taggataaag aggatgatca ttccattagg tctttgaagg gatgattttc ctctttttgt
      241 gtattttttt atagttaagg aggaatgtaa gtatggcatg gatggaatta atcatagcag
      301 gaattttgga agtattctgg tcaacgatga tgaaatggag tgatggtttt tctaaaatca
      361 attattcttt ttacacagtg attggcatga ttgctagttt ttactttttg tcaaaggcaa
      421 tcaaaagttt gccgatgagt ctctcttatc ctatctggac agggattggc gcagttggtt
      481 cggtcataat tggtgtggtc ttgtttcatg ataagctgaa tctttccact tggttctttg
      541 tggggatgct actcatcagt atcattggca tcaagattac tagcggccac tagacacttg
      601 gaataagtac tgatcagaaa aaataagcct ttttatttaa tattcaattt ttttatgaaa
      661 aaataagaaa taagtccaat gacctagcga cttctccact catcaggtgt atgcttgtac
      721 tatctataga aaaggagtga atatttttga aaagtctttc ggtccaagaa ttatccaaga
      781 actttggtca caaacaagcc ctcagcgatg tcagttttag cttatcccct ggcaaaatcg
      841 ttggattagt aggtcctaat ggcgctggaa aaacaacgat catgaaagct attttaggac
      901 tgatccatta ctcagatgga acaatctgta ttggtgaaaa tgagatttct ccctctagtc
      961 ataaagggtt ggaacaagta ggagcattga tcgaataccc gggtatctat ccattcttaa
     1021 caggctatga tcatctgaag ttattctctg aaaccaacga tgtatctgct atcgacacta
     1081 tcgtgaatca gctcaagatg gaaaagtaca ttcacaaaaa agcgaaatcc tactcgttgg
     1141 gaatgaaaca aaaattggga attgctcttg ccttactgaa ccatccgtct atcgttattt
     1201 tagatgagcc gatgaacggt ttggatccac aagcaacaag agatgtgcga aaaatcatta
     1261 ccgacttggc tactcagggc acctctttct tgatctccag tcacttactc agcgagcttg
     1321 aaaaaatggt cgatgagctg atcctgatca atcaaggaaa gatcatcaaa caatgtacaa
     1381 tggctgaatt gaaccaaaca gcgcaagatt tcatcgttat ctctacaaca aatgatctag
     1441 ctgctcgaac gatcttgaca gaagctggat atactttgga agatcctgat aaaatcaagc
     1501 ttttaaaaga aaacgaagaa ttattaggaa ccgtgatcca gctattgtct gcacaaaata
     1561 tccatgtgac agacgtccag catctgacga atgatttgga atcttctcta ttggaaattc
     1621 tggattcagt aaagggggaa gaagaaaaat gacattgctt gtaaaacaag aattatttaa
     1681 attgatcaag aaaaagtcaa ctgctgtttt atctgttcta ttagttgttt tgctcatagg
     1741 aacagcactt ctagccaaaa aatacacgac gatcattgat ccagttgaaa tgactgctca
     1801 gctttttagt gccacatctt ggattgtctt tatcatgatt gcagctgcaa gcacgatcat
     1861 ttcgatggaa gcacaatatg gtacattgaa aaatctcctt tatcgaaagt attctcgagg
     1921 agaaatatta gtaagcaaat ggatcacatt agttatttat tctgtttatt tatacctttt
     1981 agctattatt gtcactgtgt tgatgaaact gattcttttc ccttcgattt ccttcacgga
     2041 gaaggtttct actggtcaaa cactgatcca aagcttgttc acttatactt tgggtagtta
     2101 tatcggttta tggctgattt taagtttagt gttgatgatt gcttgcttta tcaatagttc
     2161 cggcgcatcc atctcagcag gaatcgtctt ttattttgct tcgtcaatca tatctggtat
     2221 ccttattgca ctcatccaaa aatgggagtg gatcaaatgg aatccgatca gtatgttgaa
     2281 tctgcaaaac caaattggaa acgaagagat catgaagcaa ttgactcatc tttctactaa
     2341 tcaaatgcta ttcggtaatt tagcttacat cgttttgttc ttagcattag gatatctcgt
     2401 atttaagcga aaaaatgtat gatgactcat caaaaaagag gctgtgaaag agacgttcag
     2461 ccttattgcc gattggctgt ctcttaaaca ccgtttattc tgaaaaagga gctgtgacaa
     2521 catttgtgtc acagctcctt tcctagtatg tttttgaata aacgacaggc tcacaacctc
     2581 tctttatact tcttttatac ttctatacgt gaagcatccc agtggcaaat aaaaccattg
     2641 caatcactcc gaccatcgtc acacctataa gtaaaatctt atctaagtgg atatgttgta
     2701 caggctcgtt ttgctcctta cgtgctttga ggtaaaaata aatgcctggc aagaaaagaa
     2761 caagaacaag cattacttca ttaaaggcta cgactagcat agcccatact tgaaaggcac
     2821 ttgctaaaag ccctactaaa aattggaccc attctttccg ctgataagaa agtttcattt
     2881 ggtacaatcc taagaaaagc cacgtgaaga aaatagctgc tgtaccaagt gtgtaagcaa
     2941 agttataagc actgtcagtg aataataatg aaagtaaaaa gacattcgta cataacgtcg
     3001 tcaatacgat agcataagtt ggtgccccat ttttgttgag ttttccccat gctttaggca
     3061 aaagtccgtc ttcagccatg tttcccattg tttcaatcgg taagatcgtc catgagagcc
     3121 aaacaccaac taaagaaatg atcaaaccgc cgttgatcac gactgctccc catttaccaa
     3181 ccatcatttc aaacacatag cccatagcag gctgcgcctt gatcgtttgc agctgattca
     3241 tcgtcattgt cccatatgga agcatggaaa ccaagacgta aataataatc aagccgaata
     3301 tccctagaat cgttgctttc ccaacatctg attgtttttt tgctcgtgaa cctacgacag
     3361 aggctccttc aataccaaca aatacccaaa gcatcaccat gatgcaactc aatacttgac
     3421 tggaaaacgg gatcggctgt ccattcgcat tcaatataat gtcgctagaa aagttatcaa
     3481 tgaattgttg gatcgaaaac gtgctgatag ctactacaat aaataaaaat aatggcacta
     3541 atttacagaa tgtgacgata gcattgacga aactagcatt ttcgactccc cgattcacta
     3601 gccatgcata tccccataaa aagactgtac tcatcacgat cgatgccaga ttttgcccac
     3661 cttcaaatac tgggaagaaa tatcctaatg cgctcatcaa caatgttgca aaagctacgt
     3721 tgcctaacca gcatgtgagc cagtaacccc aaccgctgat aaaaccagcg aaacggccaa
     3781 aaccttcttc tgcgtaacta aagatcccgc cttttaaatc tggacgtttt ttattgatat
     3841 tattgatcga caaggagagc atcaacattg ctgttcctac gagtacccaa ctgaaaatag
     3901 ctgggccgcc agccgatccg gagatgtccg tcataatacc aaatacgcca cctccaatcg
     3961 ctgaaccaat tactaaggca gcaagtggaa ttaaaccaat tccttttttc tgttgctctg
     4021 tatccatgta agcatcctcc aaaataaaac cgcttttatc aaaaagtata acaaaccttt
     4081 tttattttta cttaattaat aaaaacttta atagaaatat aattctaccg gcttttttct
     4141 cattatacaa gcaaagcgcc tagtcccttt ccaaattaca ccaccaaaaa acttaaaaaa
     4201 gatgctatac taaaaggaaa gaattggagg atgatccatg aacattttat atatatcgat
     4261 ttcaggaaat acgcgtgcct ttgccaaaca tctagcagaa tatgctgaaa aaatgcatgc
     4321 agaagatcct gtcaatcctg aagttacttt aaaagaaatc catgaaaata gtgattttac
     4381 gaaagaaaca gaaccatttt ttactttcgt tcctacttat ttagacggcg gaaatggttt
     4441 agataacggt gatacagaaa tcttgaccga aacgatgcgt gaatatctgg aacatgaaga
     4501 caatcataaa ctttgtcttg gtgtagtcgg tagcggaaat aaaaacttca ataatcagta
     4561 ttgtctaaca gccaaacaat atgcacaaac atttggcttt cctttcttgg cagattatga
     4621 attacgggga acaccatctg atgtgacacg aatttatcag attttagcag aaaacaataa
     4681 ataataaaaa agaagaagct gaaaaataga tttcagtttc ttctttcgtt tctattgttt
     4741 ctatcatatt aagctttgac atccatcaca ggtgcttttg cttttgcttt acctgttttt
     4801 tccaagacaa agttttttac acgatccata ttggttacca agacgatcat ggatgtttct
     4861 ttgcctgctg ctttgatcgc ttctaagtca gcttctgcta caagcgtatc aggtgtcact
     4921 tgctgccctt ctgttacttt cacatcaaat ggcgtaccgc ctaagtcaac tgtgttgatt
     4981 cccatatgaa gcaagatttc taaaccattt ggcgttgtga tcccaatagc gtgtttcgtt
     5041 gggaagacag agctgactac cccagtaact ggagaataga tatgtccgtt ttctggttct
     5101 acaaagtagc cttcacccat catcttttgt gcaaaaactg gatcacttgc tttatctaaa
     5161 gaaacaactg ttccgtcagc aactgatgag aggatagtca cactttcagc tgaagtttga
     5221 ggtactactt tttcaggcac ttcatttact gtggctgttt cagtcgctaa ttcttctttt
     5281 tctaaacggt ctttaggaat accgaagaag aatgtagcaa cgaagccacc agcataagct
     5341 gccaataatc caaggatgta gcctaaccat ttaccgtcag agatcaatgg gatcaaagcg
     5401 gctcctgaag gtccaatagc gatagccccg acatttccga tcattccgat cactgccccg
     5461 ccgatacctc caccgataca agcagtaata aatgggcgtc ctagaggtaa tgttactccg
     5521 taaatcaatg gttcgccgat tcctaggatc ccgactggta aagctccttt gatcatttct
     5581 gttaattctt tatctttttt gcagcgaatc cataatgcca atgctgcacc cacttgtcct
     5641 gcaccagcca tcgcaagaat tggaagcaat agcgtacgac cagtttcatc aatcatttgc
     5701 acatggattg gagtcaaaat ttgatgcagt ccgaacataa ccatcggcaa gaataatgtg
     5761 cctagaacga agccggcaaa tgctccacca acgccaagta cccagttgat cccaccgacc
     5821 atgccatcag aaatgaaacc tgctaaaggc atgatgaaga aaatttcaat cagaccgatg
     5881 gcaatcaatg aaagagttgg tgtaacgatg atatctaccg catcaggtac tactttatgc
     5941 agttttttct caacgattga caatagccaa acagcgaaga tgacacctag gatcccacct
     6001 tgtccagctg caagagcttc accagtaaat aagtttttca gtggtgcttc cggattcatc
     6061 cctgtcagca tcacaacggc cccgataact ccaccaagag ttggtgttgc accaaaaact
     6121 tgtgcagcat tgatacctgt atagataact aggtagctaa acatcccgtt tttcaagatg
     6181 ttcatcacat cgatatattg ttgccatgtt gctgcatcaa gtgttccggc tgtgactaag
     6241 tttgctaaaa ctgcggcaat ccctgccacg atcccagtac cgacaaatgc aggaatcatt
     6301 ggcacaaaga tattagaaat gtcttttaat accgatttga ttttagatgg tttttgtttc
     6361 gatttttgtg ccgctttcat ttcttgtgcc cgttcgttta cacggtcttt tccagatgct
     6421 gatgaatttg ctgtagctgt acttacagaa gaaagttttt ctcctaattt cacgcctgcc
     6481 aaatcaacca tttgctgtgc tactttgttc actttcccag gtccgatgat gacttgaagc
     6541 tgttcatctt ctactacacc aagcactcct ggaatcgctt ttaattcaga gacatttaca
     6601 agatcatcat tgatgatctt catacgcaca cgagtcatgc agtggacaat gctttctaca
     6661 ttgcccatgc cgcctgcccc tttataaatt tgttttgcca gttcttccaa agtcagttcg
     6721 ttttttgcca tggtcttttc cccctcataa ggtccctttt acaaatccgt ttgcttcagc
     6781 taattttgct tgagcttcct ctttatcact atttgtcaaa atcatgacga tagccagctt
     6841 aacattttga tctgcttctt caaagtactt ttcagctgtt tcgtaatcag cttctgtcgc
     6901 ttgcatgatg attcgttttg agcgttcgat tagtttttca tttgttggtt tgacatcgac
     6961 catcaagtta ttgaatactt tacctatacc aatcatactg atagtagaaa tcatattcaa
     7021 gataagtttt tgtgctgttc ctgacttcaa tcgagtagag cctgtcaaaa attctggacc
     7081 tgcatcgact tcgattggga aatccgcgtg tttactgatt tcggcttgtt tattgcaaga
     7141 aatcgttcct gtgacagcac cgatcgattt tgcgtaatct aatccaccga tcacatatgg
     7201 tgtacgaccg ctagctgcga taccaataac tatatcattt tttgtcaaat tcagcttgat
     7261 tagatcttct tgccctagct cataagaatc ttcggcccct tcgacagcta cagtcattgc
     7321 ttcttctcca ccagcaatta gacctatgac catttccggt tctacaccga atgtcggtac
     7381 gcattcagca gcatctaaca cacctagtct tccacttgtt ccggctccca tataaatcag
     7441 acgtccacct tttttaaagg actcaatcgt gttagcaatc actggttcga tacttgtcag
     7501 ttctttttca accgcagcgg ccactttctg atcttctttg ttcattagtt gaacggcttg
     7561 agaaatactc atctcatcta atccaaaagt ttcttggttt ctgcgttccg ttgttaggtt
     7621 ctctaaattg attttgctca tttttatctt ccttcttttc ttataaattg atagttcata
     7681 ccagctttga tttggttgat caatggtagc tcttctttga taactttagc tactacattc
     7741 actttttcat ccgctggcaa atcatatttg gttaattgga tctcacccat atagcgcaaa
     7801 tacttttcgt tatctaaagt aatagaccct ttcatacgcg tagctgtatt tcttgctggg
     7861 ataggcggaa tctctcgaaa acgtgcatct acgcttcgta tgacatctct tgcatcgtct
     7921 tgccgattcg tatgcttccc taatatatat tcgtaaaatt cttcatctac aggttccgta
     7981 tggagcaaga ctgtctgttc tttttggaaa gaagcaaatt gctcctgaac gttttctgac
     8041 agacctgcat ctccgatgta aacaatatcc gtgttacttg ctaatagatc tagagcgcct
     8101 gcaagaggat ggatccctcg atgttcttcc aaagttggca atccttgata tagtgggcct
     8161 ctcaactctg tatctcctgg tacaaaacct tggacaagga gaccttgaat tttgagccat
     8221 tgattttttt tctgatacca ttctttgtcc agccctgttt cgggacgtgg ataataatta
     8281 tgccaagctt caatctgtga gaaatcagct tctgcttctt tcaattcatc gatatcttta
     8341 ggcgtgatcg tgctagcatt caaactgatt ttcaattgat gtgaccagtc ggcaatctgc
     8401 tgattggaaa tatgataatc cattcttaaa cctgttacac caattgcttt caaaggcgtt
     8461 agatcttcta atgaaaaacc cgctcgttcc aatgcctctc ctgaaatatc gaccatcaat
     8521 tctagtttat tctttttcgc ttcgcttcct agttcgatca atcgcttttg atatgccgaa
     8581 acatcatctt ctggtatatg catggaagtg aaaataccga caaagccatt ggatgccatt
     8641 tggcggatat actgtttctt ctcttctgac aaagaatcgt tcataaatat tgaaaagcca
     8701 aacatattat ccctcttttt ctgtatccgt cttcatttct atgactagag tatatatcta
     8761 aagaaacaaa atttcaatac ttttattaaa ataattaaat ttcatttcta aaggttgagt
     8821 ttatattatc aaaagaattg tcataaccta aaaaaggcac tgctaagcaa aagcagtgcc
     8881 ttgaaatatt ttatttaaat aacacaaaaa cataattact cataagaaac caattctgta
     8941 ttatctacca tgaatgtaaa gaacccctca ttaggcatcg gatgaaaata agcattgatc
     9001 actcttatca aggcattatg gcaaactaac agatagacat tttcttcgtc ttcgatgcat
     9061 tctttcaata aaggaacgat ccgtgcatac acatctaaga ctgactcccc atttgggaat
     9121 cgtacagcga acgctaaacg tgctttttga aagttctcat ctttgcttgg cagtccatca
     9181 taatccccaa aatccatttc taccaatcgt tcatcaacgg tcagcggcaa gctgagacga
     9241 tcagcaactg cttgtgctgt atctcttgct cgttgaagag gggaatggat gattttcgtg
     9301 attggaacag gcaaatctac taccttttct gctagtttct gtgcctgcat ctgtcctttt
     9361 tcagtcagcg gcacatctgc ccgtccgcaa actaatcctt gtgcgttcca agttgtttct
     9421 ccgtgtctcg ttacataaag catgtgattc tctccttcgt tggcaaagtt ccccttaacc
     9481 ataccttgtt gttcttgaga ttacaagggt tagattctct caagagaaat aatctcagta
     9541 gatactttat ccaggaaggt ttgatcatgt tccactagta acatggcagg tttcacgttc
     9601 aagatcagtt gttctaattg ttcttgattg aagacatcca aataattcaa tggttcatcc
     9661 catgtatata gttcagctgg ctgtgataaa gatttagcca attccacttt tttccgttgg
     9721 cccatactca tctgctcgat cttgttatga aaaacatctc tttccatccc aagctttcgg
     9781 aggttgttca aaaatgcttg gtagtctact tggtttttct ctgcaaattc cgccaacgtt
     9841 cctcgattgt cttcataatt ttggcttgca taactaatgc tcagatgttt tggctggtat
     9901 ttttctccta taaccttgcc gttgaatgcc cccagaagat aatggataat ggatgacttt
     9961 cctgcaccgt taggacctga aatcgccacc cgctgatgag gctcgattgt aaaatgaatt
    10021 ggctcgaata acagattttc ataccctaat tgaagatctt ctacgcttaa aagtcgctta
    10081 tggtgagacg cttggctatt catcgtcaaa gaatcgatat attcgatatc ttttaatagt
    10141 ttttctttct cgctgatctg ggtctccatc cgattcacga ttgctttgga tcgtttcatc
    10201 gtccgtgcag catcagcacc cactgctcct ttattcactc gtctagattc agtatcgatg
    10261 aatccgactt gtttctttgt tttatctcct tctcgggaac gagaccattc ggctttttca
    10321 gctgctgttt tctttagcct actgacttct tttttcaatt tttcattttg agccatttca
    10381 aattcatcac gaagtttttt ctgttcttca tagatagaga aattcccttg ataaagttcc
    10441 agttgacttt tttcgattgc taaaacatgg tccactactt catcgataaa tccccggtca
    10501 tggctgacca cgatgaagcc ttgttttttc tttttcaaat aagccgctac ttgttttcta
    10561 ccagagatat ccaaatgatt cgttggttca tcgattaacg ggaaatgagt gtcatccaca
    10621 aataaaagtg ccagtaggac ttttgtcttc tctccccccg atagtgtgct gaattctctc
    10681 cataagattt caggatctgt ttgcatcaat tggagctctc tttcgatttc ccatatctca
    10741 aaatccgtaa tatcatttaa cacgtaatag gttaaacgtt ctttatcttt tgtctgttgc
    10801 gggaaatagg caaattcttg ctgatggatt acttgccctt gataaggtag tttgttttgt
    10861 agaatattca gtaaagttgt ctttcctcga ccgtttcgtc cgataagtcc tagtttccat
    10921 tgtgtgtcaa aatttagatt tgcttgttca aataataatg tgccttggct gtcgtagccg
    10981 aatgtcagat tttttatttc gattttagac attttccatc acctcgtaaa aaaagaccag
    11041 tcctattttc agaactggct aaagaggatg caagggttgg atacacacaa aaagacccat
    11101 acatcgaaaa aaccaagcca atcccgaaaa tctgcacact gcttccttgt ctccacaagg
    11161 tacagtctga atgcagcttt catcaatcga cttaattttt caatgcacgg atccctcact
    11221 ttgtcctata tttgaaatta cgatagcata aagaaaatga ttttgtcaac tacttcggga
    11281 ggatggtgaa aaatctgcta agtgtctgtc ctaagatttt tgttttttct aatgtttgcc
    11341 tgtgttccat tgcttcttct aaacctattg gacccaaatg aatacttaag acaattggga
    11401 gaaaatcaga aagtccttct agctcttggt ctctacttcc gcaaatagcg attattggtt
    11461 ttcgatattt ttttgcaagc actgctacac cgtatggaac ttttccgtgg atcgtttgct
    11521 gatcgatccg cccttctcct gtaatgatga catctgcttg ttcgatcttt ttttccaatc
    11581 cagtgatttg gctgactaaa tcgaaaccag aaaccatctt cgctcccaac aaaaaaaggg
    11641 ctccaccaat accgcctgct gctccacttc ctggaatatc atctaaatcc acctcccaga
    11701 aatcttttat ttgctgagca atctttcctg cttgtttatc taagaaagct agcgtcgtat
    11761 ctattcctcc tttttgtctt ccaaattgcc aagcagctcc ttgttgacct gtatatggat
    11821 tggtaacatc tgcagcgata atcaatttta ctttagaaac aagaacgttt cttcgatcaa
    11881 aatgaagttt tttagtagat aataaaggat ttccttctgt gtacccttct aaagaagcac
    11941 ccaaggattg gagtaatcct agaccaccat cgctacaccc gcttcctcct aaagtgacaa
    12001 taatttgttc tacttcttga agcagtgcat ctttaataag ttctcctaag gcataactgg
    12061 aagcaagtcg gatagtttga tccgttggtt tatcaagtag atctaacccc aatgcttgtg
    12121 cagattcgat gactgcggtg ttttttcctt gccaagttgt caataaatag ctgatttttc
    12181 gctgacggaa taagagatcc atcccatcaa tggtacgcca tgttcccctg agcgcgtaat
    12241 agattgcttc tgcagtattt tctccgccat ctgcaatcgg cacattgatt tttcttcctt
    12301 tacgatcttc ccagccattt agaactgcct gatttagttc aaatgaagaa gcacttcctt
    12361 taaatgaatc aattgccgcc aaaatatcca tctgtatccc tcactttctt cttaagtaag
    12421 acactttttt tcattttttt caaacaaaac aatgctattt tcttccattt ttctatatac
    12481 taaaagggaa ctcattgaat aaaggagaga aaaaacatga aaatcgcaat agctggggcc
    12541 ggagcaatgg gcagccgaat cggattgatg ctacatcaaa gcggaaatga ggtcctcttg
    12601 atcgatcgtt ggcctgctca cattgaagcc attcgtacaa atgggttgat tgctgacttt
    12661 aacggtaaag aagtggtcgc aaaactgcca atctattcac cagaagaaat catcgaaagc
    12721 aacgaacacg tggacttgat cgtagcatta acaaaagcca atcaattaga tgatatgttt
    12781 tgttcgatcc aatccattat tactgacaat acttatgttt tgtgtttact aaatggatta
    12841 ggtcatgaag atgtactgga aaaatatgtg ccaaagaaaa atatcttatt tgggatcacg
    12901 atgtggacag ctggtctagc tggtcccgga aaagtcacat tgttaggtga tggagaaatc
    12961 gaactagaga acttggaacc agaaggcgaa gcatttacaa aaaaagttgt agaagtcttt
    13021 caagaagcaa atttaaatcc gatctatagc cataacgttc gttattccat ttggcgaaaa
    13081 gcttgtgtaa atggaacatt gaatggttta tgcacgattt tggattgtaa tattgctgaa
    13141 ctaggcgcgc aaaaagcggc agagagcatg gttcgaacaa tcgtatctga atttgcttct
    13201 atcgcagcaa aagaaggaat catactagat caagaagaag tgtaccaaca tattgcttct
    13261 acttatgatc ctgacaacat cgggttgcat tacccatcta tgtaccaaga cctgatcaaa
    13321 aaccatcgtt tgactgaaat cgactacatc aatggtgcca tttggcgaaa aggacaaaaa
    13381 tatgatattg ctacaccata ttgtgccttt ttgacccaat tagtccatgc aaaagaagga
    13441 atccttggcg cagaataaaa ggctctctga tgagaatcag ctgaatcagc agctggttct
    13501 ctttttttaa gcccaataaa agatcaaaaa gaaaacacaa ctctcatgat gccataagaa
    13561 ttgtgcttgt ttgtttcagt acataatctc ttcaaaatgt aggtttttta tttgttttca
    13621 acagaggaga ggtcatattc gtattgctca taaatagtgg aatcgttagt gataaatcgt
    13681 tttgcccaat tttctgcatc ataaaattcc aatagctctg ataggattgg cttaaatggt
    13741 gtatgcagaa attccggtaa ttcatctaaa tcacgttctc gaagcaaaaa aatattttca
    13801 ggacgataaa aatgataatg agcaatcgct tgattatccg tcactagttt cttttgaaaa
    13861 tacatggatt caaatgtccg caaagtcact ccttgctgac cctcccgcaa cagttccaga
    13921 atcccgcgcg attcccctgt ttttttcagg tactcttcat aagtaagata tccaggttgt
    13981 tcattccctc gatgtggaag aatgaaatag ttggtcgtaa catctctttt tataaattcc
    14041 tgcttatagc tttctgctcg ttcttttctc ccattattcg ttgcaccaaa aaatagatcc
    14101 gtctttggtt cgctagaaga aaaattcata tgtttcgaat aaaatgaact attatgtttc
    14161 agcccaagtt cttttgcttc caaaaaatca aaatgataaa tctcatcgat tgccggttca
    14221 tcttgtgcct gcttcagcat cgcctcgtgt tctgctttga agtgattcca aaaaaataac
    14281 acaatttttg cattggtaaa tgggcgcacg cgtcgagcca tctccatatc aaatccataa
    14341 tctaaaaaaa cgatcagctg ataatttgcc aactgttgtc tagctgattt aatgaattcc
    14401 gactgtagta aatcttttcc ggaccaaaca tctaaagagc ggatggaagg caaaaaataa
    14461 agtgatttat tttttgcaac tagcagtacg tcatttttgt tcatcttttc cgcctcactt
    14521 tctctgtcac tttttcctat gatagcctgt ttaaatagta aaaagccagt aaaacgtttc
    14581 ctatatttgt atgaaaattt aacatttgta tgaaaatatg atgaaataaa tcattcttct
    14641 attaaaattc gcctaaaagt tttctgaata gttgtaattg tctgaaaatt tgttatcatt
    14701 agtcattgtt aaatattgct atttattctt aatataaatt ttgattgtga atttaaaaga
    14761 aagaggtcat ttatggaaga taaaaaattg aaaaaacaac ttagctccag acatatcacc
    14821 atgttagctt taggtggagc aatcggtgca ggtctattta aaggaagtgg cgaagcgatt
    14881 gggatcgccg gcccttctgt attgattgct tttttgattg gtgggctgat tttatatatc
    14941 gtgatgaaag gtctgggaaa aatcgtttta agcggtggcg acacgcatca cggattatct
    15001 ggtttgatac gcccttatct aggtgctcat tctgctgatt ttactgattg ggtctactgg
    15061 tctatgtgga ttatcaatat catcgctgaa gctgttgcag ctgcttcctt tttgcagttg
    15121 tggttcccgc acattccagc ctgggtattt gtttttatgt tggctgtttt aactacgatc
    15181 atcaatcttt attcggtacg gttattcgca gaaacagaat attggctggc ttttgctaaa
    15241 atcagtgtca ttatcttact gatcattttt gctacttatc ttgtcggtca gcaaatgttg
    15301 ggcacaggcg ttttccctac ccttcagcaa ttgactgacc atggtgggtt tactcctcat
    15361 ggtatgaagg gaatcatcag ttctttgctt gtagttatct attcttacgg cggttctgaa
    15421 ctgattgcta tcacggtaag cgaagcagat gatcctaaac gtgcgattcc taaagcaatc
    15481 aaaggtgtca tgggaagaat catttccttt tacatcattc ctttgttttt gcttttgatt
    15541 ctttttcctt ggaatacttt agcaggcacg aatgtcagtc cttttgtgat ggtatttgaa
    15601 aaaatgaata tcccatttgc agcggatatc gtcaattttg tgattgtcct tgctttgttc
    15661 tcatctatca attcgggtgt gtatgcatct tcaagaattt tgttcttccg tttgaaggat
    15721 cgaaaaggtt cctctagaaa actagctgtt ttaaacaagc atcaagtacc gcaacgtgct
    15781 gttttatttt gtaccagtgt tctttatctt ggtgtagctc tttcttattt tgtcggtgac
    15841 aaattatttg gctatctggc aggttccctt tcgtatacag tattattgat ctggatcatt
    15901 atcagtgcgg catcctttgt actggcacta agaaaaggaa cattatggga taaaggaatc
    15961 agcttttttg cattagctgt cttaggattg attttccttg gtatcctgtt cacgaactcc
    16021 attggggtaa ctgtattgac tggcctgctt tacctgttca tttatttcag ttatcaaaaa
    16081 aagaatgatg cgttcgtatt gacgaatgaa tgaaatcgat agaaaatttt attttattta
    16141 tgtggaatag atggagctat tgaagaaagg agttgtgaca aaagtcttgt tgcaactcct
    16201 ttttccgaat aaacagtctc atgacctctt ggtaaacggt gttcaaaaac cctctgtgca
    16261 aaataattga ttgttctcca tgctcatgac atgatagaag aaaggaatga aaaaaaggag
    16321 tttgttttaa atgaaaaagt attggaagtt tttgttgatg atcggtgttt caacggtagt
    16381 tatgtttggt atgatgtact tgaatgtcta tgcgctcgat catatcttct tcagtcgtac
    16441 acgagtattc atggcgttga tgatgggtgc agtcatggca gtcatcatgc tgttgttcat
    16501 gtggaaaatg tatgaaaaca aaaagataaa tgttatgatc ttagccgcta gtcttcttgt
    16561 ttttgccggt tccttgttta tggtacgaag ccaaactact gtcaacgaca cagcttggat
    16621 gaaggccatg attcctcatc attctatcgc tatcctggca agtaaacgag ctgatttaaa
    16681 agatcctgaa gtaaaaaaat tagcggaaga aatcattcag gcacaagaag aagagatcga
    16741 caaaatgaaa aaaatgatca aagaaatgga atgaccatta tccgtccaaa taaaaaaaga
    16801 ggatgttcca gaagttatca gcttcaagaa ataagaagaa gctacttctg gaacgccgtg
    16861 aataaggggt tgtgaaaaag ctgccctgca tcaagtaata agaacagcca aacaaaaata
    16921 gcttctccta tttttcgttt ggttaggctt attaccgcag atgcaagctt ttgaacaccg
    16981 tttattaaga ggttgtaaaa tcagcgttct gacctgagta ttagagagac aaatggaaaa
    17041 atagttcctc atattttgcc attttttgag ctaataccga gggtcagctg attgaacacc
    17101 gtttatcgag aggttgtgaa aaagtcgctt agtttcgcgt attaagaaaa attttgtgaa
    17161 atggctttcc acatttttgt ataaatttgg cttaataccg agaaactggc ttttgaatac
    17221 cgtttattcg taaaagggat tgtgacaaaa gtcttgtcac aatccctttt tatgttttta
    17281 ttgttctaat tgcgctgtct tgcttactgc ttcttgcggt ttcacaaatt tttttgagat
    17341 tccccaacct gtgaaggcac tgaccatcga aaacacagga gaaagcaaac tgaaaaatac
    17401 gaatggcaag taagtgagtg taggaatacc aaatgtactt gctacgaaac ttccggcaat
    17461 tccccaaggc actaagtagt tgattactgt tccaccatct tctagcgttc tacctaatac
    17521 agttggatca aatcctgctt tttcataagt ttctttaaat gcattaccag gcagaataac
    17581 agataggaat tgttcaccaa taaaaatatt tactccgata cttgtcatca atgttgtaaa
    17641 aatcaaattt ggtgtgttcg tcatctttct tgatactcgt tccatcacgg ttgagatcaa
    17701 gccaaattcg atcaacaatc cacctaatga caaagttaga ataatcaatg acacagtagg
    17761 catcatactt tcgattcctc cgcggctaag cagtgaatcg atttgttggt ttccagtatt
    17821 tgaaacaaac cctgattcga tgaccccagc caacttcgtt aaggaagttt gtgggttttg
    17881 aataaaaatc attcctactg ctacagcgat attcaacaaa atcgttaaaa ttgccggcac
    17941 ctttttccat gcacacagta acattaaaaa taaaggaatc agtgaccaga ttgaaatagt
    18001 aaagtttgct tctagaacat cagtcacttc acgaatagat gaaagacttg cggctttact
    18061 tgtgtgtcct aaaatcatga ataaaaagaa agaaacaaca aatgccggga cagttgacca
    18121 catcatatgc ttgatgtgtt tgaacaaatc ggcatcaaca atagctgctg ctagatttgt
    18181 tgattcggat aatggagaca tcttatctcc aaaaacggca cctgaaacaa tagctccaac
    18241 aaccagcgct ggatttaagc ctagtgttgt cccaataccg aaaaatgcaa tcccaatcgt
    18301 tgacataaca gtaaaggcac tccctacagc gctacctacg attgcacaca caacaaatac
    18361 agaagggaca aaccactgga cactgatcaa tttgaaccca atgaccatta gagttggaat
    18421 gatccctgct tgtatccata cagcaatcaa tgaaccgact aaaataaaaa taaagattgg
    18481 gatgattcct ggtttgatgc cattgatgat tccttcgtgc agccaatctg tcgggtatct
    18541 ttttatcact gcatacaaca tgaccattcc gatcgctgct aagatcgtga tattaggtga
    18601 gacctttaat ccgacaacgc caatcgcaat gcctaaaaca ataaccatta atacaagtgt
    18661 tgcttctaaa aaacttggtg ttcctttttt tcccatgata atctctcctt ttttagttgt
    18721 tcttatgttc taaaaaagtt aagatatcgg attactccgc cgcaattcat ccgcgattcc
    18781 tcctttataa aaataaaaag acctgtttta aactaaggac gaatgactcc gcggtaccac
    18841 cttaattaaa aacagatctg tttttcactt tcaaactttt attcaaatgt atggactttc
    18901 catgtaattc gtcaattagg cttgatcggt tcacagcgac caccgacttt ctgaacaaaa
    18961 cacctaaccg ctactgcgtg aaaagttgac ttgtacgaag tttaccttcc ataagatcat
    19021 ttgtcaatga aaaagaaaat attttttcat ttttctgaaa attattttta taactttcac
    19081 acacaagttt attttttact agaaagtagc gccctcttgt ttttttcttg ctactatttc
    19141 ttgatgttct tgcttgattc tttctaatag ctttggctct ttcaacagat caagagctgt
    19201 attggcgagt agttctgctg caatcgcaat agaagcaagg cctttgtcac ttttagcagc
    19261 agctttgaat tcttctgtgt gtccggcaat atatgtctca gagattgcta ccgtaggctg
    19321 gattgttggt atgacttggc tgacatttcc tacatcagaa gaaccgagac ttgttctttg
    19381 ttccgtttct atttcttcat ctggaacacc tgcttctttt gcatgctgga agaataagtc
    19441 atcaaaagaa ggagtaacga tgacatcatc aactgcattt tggaacaatc caaactcaaa
    19501 agtcgtgccc gtacttatcg cagcacctcg tacgatattt tccacttttt catatacttt
    19561 atctaatgtt tgccgatttc ctgcacgtaa atagaaacgt ccagcagcgt aatctggaat
    19621 gacgttggca gctactcctc cgtctgtaat gatcccatgg atattgacat cttttggcaa
    19681 ttgcaagcgt agagcgttga ttccattaaa cacttggatc aatgcttcca aagcatttac
    19741 tcctttttca ggtgctgctg ctgcatggga agcgacaccg taaaatttga tgtcgactgg
    19801 atcattcgct aaagattctg tcgttttccc ataacgaaaa gctggatgga cgcataacgc
    19861 tacatcgaca tcctcaaaaa aaccttctcg aacaaagctg cctttagctg atccgttctc
    19921 gcccccttct tcccctgggg tgccatatac acggatttct ccaccagttt catcaatgaa
    19981 ttgcttcact atgctggcgg ctaatacgga gtaagtacca aacaaattat gcccgcatgc
    20041 atgaccaata cctggtaaag catcgaattc tgccaaaaag gcaattgtgg gaccgggttt
    20101 attggaaata taacgtgcat caaaggctgt tcggtgtcca gctacttctt tttctaccca
    20161 aaacctttct ttttctaatt gtttaatcaa cgctgctgag gaataaaact catagttact
    20221 tacttcagga tgttcgtgga tatctagagc aagtgcttga tattctggta aacgatattg
    20281 gattagatcc ttcaattggt ttgttgagat tgttgttgtc ataaaaaatt cctcctatgc
    20341 tagtcatttt tcttattttc tcccgaatgt ttcccatgca ggcacgcttg atccttttga
    20401 tgttttctcg attactttga ctgtgtcttc tgtttgataa gcgtctacta ttttttgata
    20461 cgttttattg ttttcatctt cttttcttgc aacgatgata ttcacataag gacgtgcatg
    20521 gtcatccact ggttctaaga agatggcatc agaagcagga ttaaatccag catctacagc
    20581 tacaccgcta ttgatcactg aaatgtctac atctgacaaa gcacgtgctg tttgggaagc
    20641 atctaattca gaaatgtcta gattttttct attctcggta atatcagaaa cagtgggcgc
    20701 ttgtcctttt gccttatcca ctttgatcaa tccagcagtt tgcaataaca gaagagctct
    20761 gccgccattt gtaacatcat tagggattgc tacttttgct ccgtctttga tatcagctac
    20821 atccttcaaa gtgttagaat aaattcccag cggtgcattg acagtatttc cgatcgatac
    20881 taaatcagtt ccgtgttctt tattgtaatt gtctaaaaag aattgatgtt ggaaagaatt
    20941 caaatcaatc gatccatctg ctaaagccac atttggttga ttgtaatctg taaatttcac
    21001 gtattccaaa ttaatgttct cctttttcaa acggtctttc acgttgtccc atacatctgt
    21061 atcttctcca ataatcccta gttttacgat agtttctttt ttgtcagttg tatctgcttt
    21121 tccttggcta caccctgcca accaaataat tgctacaaat gtaatgacta caaaacgaat
    21181 aatttttttc attttttctc ctccactttc aatgtgtaat ttttttaatc agttgattac
    21241 tgataaattg acttaaaaaa actaagatta gaatcaataa tgtagccaca atcgttacat
    21301 ctgtttggaa tcgattatac cctctcgata tagcaagatt ccctaatccg cctgcaccta
    21361 ctgctccagc catggtagtc agtccgatca cgttgatgat agtcaacgcc gagacccgaa
    21421 tgataccagg caaaccttct aacaaataca cacgtaaaat gataccgatt ggactggttc
    21481 ccatcgcttc tgccgcttcg atcaccccag gatcaacttc tagcagtgcg ttctcgattt
    21541 gacgagcaaa aaacggaaca actccaaaaa tcaatggcac taaagctgct ttcgcaccaa
    21601 tcgtcgttcc aactaagaaa cgagtagtca atgctaaaag agaaagcaag ataatgaaag
    21661 ggatggaacg aatgatattg atcagcttat caagtatttc aaatagcaga cggttagcta
    21721 gtaatccatt agggcgtgtc actactaacg tcacacctaa gatgattcca atcatcccgc
    21781 caatgaatgc tgtccaaaaa gacatatata gtgtttgctt cgtcgcttcg acaaattcat
    21841 ccggaatcaa ataaacattg ggcaaatatg ttttaaagaa atcagtcatt ttatcttcct
    21901 ccttcgatca ctttgatacg actatctaaa tcgttttttt gtaaaatatt tatttttaca
    21961 ccagatttga tcaaataatc caatgctttt ttccgctgtt tggtttctcc agctaaagta
    22021 acgatcaaac tccctaacgg cgtttcttgt aataactcta cattgccata taaaatattc
    22081 gctgttactt gatatttgct gtacagatga gcgattagag gttcgttcgt ctgatttcca
    22141 atataactta attccaccag ccatttattg gcgatctgat cggcaaaagc agaatgggca
    22201 atgatcgttt ctaatgcttg gtcaagatga gtagctgttc gaataaaatc tttggtcaat
    22261 tcttcttcag gattgctgaa gatctggatg ctgctacctt tttcaacgat tcttccatct
    22321 tccataaccg ctaccttgtt gcagatttct ttgacaactt gcatctcatg agtgatcagg
    22381 acgattgtta aacctaactg gcgattcaat ttttttaaca atgcaaggat ctgaagggtc
    22441 gttttcggat caagtgcact ggttgcttca tcacataata ataccttagg ttcattcgcc
    22501 aaagctcgtg cgattgctac tcgttgtttt tgtccccccg acagttgttt aggataagca
    22561 gatgcttttt cttccaaacc gacaagttcc agtagttcat tcactttttg acgacgttcc
    22621 tgtttcgatt ttccagaata ctttaaagaa aaatcaacgt tgtcaaaaat agttcggctt
    22681 tccattagat tgaaatgctg aaagatcata ccgatggcct ttctttcttt tcgcaattct
    22741 ttgggagaga gctgactcag attcgtctga ttgacaatga cttctcctgc tgttggtttc
    22801 tgcagcaaat tcatcacacg aactaacgta cttttccctg cacctgagta accgacgatt
    22861 ccataaacat cgcctttttc aatcgttaaa tctacagaat ctactgcctg tatctctttt
    22921 tctttttgtt tgaaacatac agaaacatct cgcaattcaa tcattaaaat tcctccttat
    22981 ctaaaaaaag cgtcctcaag aaaatgaaaa tgtcattttc ttgaggacgc ttttttgcgt
    23041 gttaccacct catgttcaag ctgtgttcac acacaactcc tcttcaagta cgatcatact
    23101 ctagcaagat atcggtcgca gccgtcatcg ccttacatct atgctcggga tgaattgctc
    23161 gatggccatt ttccaatagt cgttccttgc ttcttctcag ctaccgaagc tctctgtcaa
    23221 aggttcttct atcgtactta ccatttcaat gcaaataata ttcaaaaaaa aagtcctttt
    23281 actaacataa actatgtcag taaaaggacg acgtatcgcg gtaccacctt ttttcggaag
    23341 tttccttccc tcaagcccaa catctcaaaa agagagattg gcgttcgttt taacggtcga
    23401 acagttccga atttgcctac ttatcgacaa attattctcc aaggccattt tcagcttttc
    23461 ttacttgact cttctcacca gccagagcct ctctgcaaag tttccaaact tactttcctt
    23521 ttcaacgaaa tcgatatttt gttgttggga ttaatctaca tgaggaaaac tgaaaagtca
    23581 agtacgaaat tcaaattatt ttttctatat ttatttttct gtttttttat ttaacatttc
    23641 aataacagtt cgaagatctt ttccttccgc aagaagtttc aatttttcct tttcttcgac
    23701 ggtataattt cggacaaaac tggaatcagg tatcaaaaca acgcctaatc ctgctcgtga
    23761 agcagctgtt gctcctacta aagaatcttc cactgcaaag gctgaactag cttctaatcc
    23821 tgttcgttcc aaagctgtca gataaggaat cggtgaaggc ttgttctccg acacttcgtc
    23881 tccgaatgta aacgtgtcca agtaatcagc taatttaaag tgatccaaga tatctgttgc
    23941 tcttttcgta acagtggtcg tagcaatgcc tagtttcata tgacgttctt ttaagtctgc
    24001 caatatttct tttgcatatg gttttaagtg gatggctccg cttgtcagct gttgataaaa
    24061 atattcttca cgcttcgctc ggacttcctg tactttttca gggctacctg ctgctttcac
    24121 taaaatatca aaactttgtt tccagttttt tccgccccaa gcgtcaacga tttcttttgg
    24181 gatatggata tcaaattgct gaaatcccca aagccagcca tcacgatacg ttttttctgt
    24241 gtcaactaac aaaccatcta aatcaaatat tgcacctttc atctatacat taatcctttc
    24301 caaaacaata cacatagttt agcacgactg gtccaaaaag aacagaagca aagaaccagc
    24361 cttctcgttt ataagagttc ttggaatcga cggatataga ttttcttcac agctgtcaca
    24421 agcgccagat aagcgatgac tgtagcaaat aaccataacc agtaattgcc aggaagtggc
    24481 atcaagccta attcagcacc aaaccttgta aaaggaagga cagaaccgac aataattcct
    24541 aatgtagtga taacaaacat ggcaaatgtt gcgttgcttt ggataaatgg tactttcggt
    24601 gtacgcagtg catggagcac taaggtttgt gtccatagtg attcgacaaa ccatcctgca
    24661 tggaataatg cgatgaaaat cactcgctgt gaactatcca atgtatggaa acttccgccc
    24721 actacagcag gacaaataac aaaatacatc aacaagtaag tagtaatatc aaatatcgaa
    24781 cttgttggac caaagtaaac catgaattcc ccaatactag atgcattcca tttttttggc
    24841 tcgtccaaat attctttatc catccgatcc catggcaagg aaatgcagga aacatcatag
    24901 atcagattca aaaacaaaag ttgtaaaggc agcattggta aaaatggcag gaacgtactt
    24961 gcaatcagca cggaaaacat attgccaaaa ttagaacttg ctgtagcctt gatatatttc
    25021 atgatattcc caaaagtttc tcgtccagaa agaaggcctc tttccaaaat cgtcaaatct
    25081 ttttctaaaa ggatcacgtc tgcagattct ttcgcaatat ccacagctgt atctacagaa
    25141 ataccgatat cagccacttt cattgcagga gcgtcattga taccatctcc taaaaatccg
    25201 acagtatgac catttttacg taaagctgtc gtcaaacgtg ctttttgctg aggattcaat
    25261 tttacgaata cttcatggtc ttcagctact tgtgctaatt catgatcact catttccgag
    25321 attttctcac cggtaaccaa ttcattgatc tctagaccta cttgacggca cactgaacgt
    25381 gtaactaatt cgttgtcccc agtcagaact tttacagcta caccatgttc tttcagagct
    25441 ttcaacgctt gtttcgttgt ttcttttggt ggatcaagga aagcaagata accaatcaaa
    25501 accatgtctg actcatcttt gactgaaaat tctccaacga cgcttggatt ggtcttttgt
    25561 gcgacaccaa tcactcgtaa gccatcttca ttaagtgact gtacttttgc aagtacggct
    25621 tcttttcgtt cttttgttaa tggtgtaaca gtaccgccaa gatcaataaa tttagaaatg
    25681 ttcagcattt cttcaatcgc gccttttgtg atcatttgtg ttttaccagc tggatcagca
    25741 accacaacac tcatacgacg acgttcaaaa tcaaaaggaa tttcatccac tttttgataa
    25801 ttgatcttat ctgtatctaa agtttctgtt gcttcatcaa tgatagccac atctaacaaa
    25861 tttttcagcc ctgtttgata gtagctattt agatacgcat ggcgcaaaac acgatcatct
    25921 tcatttcctt cacaatccat atgatattca agaatgattt tatcttgtgt caatgtcccc
    25981 gttttatccg tacataacac atcgattgca ccaaagttct ggatggcatt caaatttttg
    26041 atcactgttc cttttttggc catcacactt gctcctttga ccaaattcgt cgtaacgatc
    26101 attggaagca tttcaggtgt tagaccaact gctacagata gtccaaaaag aaaagcttct
    26161 agccaatccc cttttgtaag cccattgatg acgataactg ctggagccat caatgacata
    26221 aatttgatca gtagcatgga tgttttatgg atgcctaatt caaagcttga ttgaattggt
    26281 ttttcagata gcgtttctgc tacatgacca aatagtgttt catttccaac agaaacaatg
    26341 attccttccg cacttccact gatgacatta cttcccataa agacaagatt ttcgtaactt
    26401 gtttcgcttt gctgatcgtc ttcacatact ttcgcatgct tttctactgg atagctttcc
    26461 cctgtcaatg ctgcttggga aatgaataaa tcctttgcgg acaataaacg catatccgct
    26521 ggtatcatat cccctgctga aagccgaaca agatctccac agacgatttc ttccatagga
    26581 atctctgtgt actttccatt acgttttaca gctgccgtta ctttcacaag tgatttcagt
    26641 ttttcagccg cttgattgga acgtacggac tgaatcaacg tcattgtccc gctgacgata
    26701 accatagtga agataatggc tacaccgaaa agatctttat ctccaggagc tgctaataca
    26761 tattccgtga tgaatgaaat aattcctaat gcgatcaaca ctaatgtaaa tggcgtgatg
    26821 tatgctttca atatttctac aatgaatggt gtttttttgc catatgaaat cgcattttca
    26881 ccgtaatttt ctcttgaacg ttcgatttgt tcttctgtca atccatttgc tgatgcatgg
    26941 aatagttcaa ataattcttg tttcgttttt ccagcaaaag ctgcatagat tttttgtgta
    27001 tctttctttt tttccatctc aatctctcct ttgtgtccaa aagacagctg ttctcgctgc
    27061 ttttggtctt tagctattca tcggatcgat agagaaaaaa ctgaaagggg ctgagaacag
    27121 atagataata gccctggcct ttcctctaga tccttgtttt tcgaatgact gtcatccatt
    27181 tgttctcatc tcctttacta agtaatttta attcatgact tccaatcatt tttcgaatag
    27241 ttatttttcc aattagacca cctccttttt atttttcacc gatggggatt tgcggcaaca
    27301 aaaaaacctc tcgccaaata ctcagcgaaa ggtttgaaaa agcatattaa aaaaagcttc
    27361 tcagatctca tcgttgagtt ttgacactgt acaacgtaaa aagcagatac ctgcttttag
    27421 ctaccttatg aaaagcctta tctcgacaat tcctgttcta cccattggcg tctctcgacg
    27481 tttttgggca gtagcctatg tttgtatagg agcctcacct aacaaatctt attcggtttt
    27541 ccttcgttaa tataacaact aaaaaagagg atgtcaaatc aattttccat gtttttcata
    27601 ctttttttaa aatacacacg ttatattcct gtctcacgcg gttttatttg tctcattttc
    27661 cacctcccgc tataatagaa atatagtagg aaacgaggtg ctgttatgga aagaacactt
    27721 gttattatca agccagacgg cgtgagacgc catttagtcg gaagcatcat ccaacgtttt
    27781 gaagcaaagg gactggccat tgctgaaatg aagtttgaaa ctatgactcc tgaattagca
    27841 aaagaacatt atcagcactt gactgagcgc agtttttttg atgaactgat cgattacatg
    27901 acttccggtc ctgtcgttta tcttgtgctg gtcggcgagg aagtgatcga tatcgtccgt
    27961 aagatggttg gagcaacaaa agctgccgat gctgttccag gtacgatccg aggagactac
    28021 gcgctgccgg ggactgaaaa catcattcac gcttctgatt cccgtgatgc tgcggtaaaa
    28081 gagatcgccc gctttttccc gccattcgat ataaacaaac cgtctgaaaa agcggcgaag
    28141 tgatacaaat tctaaactgc tggtctagtt gctgggttca gcaatggcca gcagtttttt
    28201 ctctaaggac ctccgttgat tctttcctaa catatattcg agcactgtga agaattcttg
    28261 gtagtctttc gtcttttctt cccctaaagc agcagaaatc aatctttgat aataatctgc
    28321 catcaatgtt tcatagatca ctgtacttct ttctttatgt gctaagctcc gtttcatatc
    28381 ttcattcaca ctacgtgcct ttttcaaccg accattttct atataaaacg aagtcaggtt
    28441 cattaaaaaa gtaaagagat actgctcatg attgttcaac tgtgctaatc gtcgcattct
    28501 ttttttgatt ttcaaataat tgttctcaat gaaccgatca ctgaatatcc aaggaaaatc
    28561 agctagaata gatagttcaa attgaccata gtctggtatc tgcatcaagt attcttcaat
    28621 gattcggcat tcataacgta aaaattcaaa ttctgtctca gatacttctg ttttttcgac
    28681 catgtgaaga ttcaaaacca aataatacag atcttctgtt ttcttaaaat gcctacggtt
    28741 attgctgatc tctttataag gatcaaagct gtcttcttgt acccgatcta gcattcgtct
    28801 ttttttcttt aactttattg gttcatccag ctggtttcgc atccattcat attctgcaag
    28861 tgtcatgttc attcgctcta aaaatgatct caatgttaca aatgacattt ttttcactga
    28921 atttgtctca atcccgacta gagaagtctt aaaacagatt ccttccgcta attgggactg
    28981 tgtcaacttc ttgttgactc gaagtttctt gatcaattca ccatcataca taatagttct
    29041 cctttttaat ttaaattttt ccacattcaa taaatagata cgttaaaaag gaaaaataca
    29101 cgaaaaaaat aaaaaaatgt ttttttcata aaaaatacca accttcaagc attagaatgt
    29161 tgtctaacta ctgaaggttg gtttgattgt ttattgatta tttttttgct gttcaacgtc
    29221 tgttcttgtt actggatata agttggttgt tgttttatta ccattttttg aaaagacact
    29281 tgtcgaatgt tcgccttttt gcttttcgat tgcttcatta cgttccgctt catttttata
    29341 gtcatatttt ttcggatcaa tcgtctcaaa tccgtttggc acataaaacc gcaagagatt
    29401 ttcctgattc agcttatctg aaagacttaa cgcagtcttg actttttcct gatctttgga
    29461 gatttcctca tcgattcctg ctgctttcgt atcgatgatt tctcctgtct gattattgta
    29521 agcttttcct cctaggacgg tgtatttggg tgtcacaaag ttcccatttc gaaaagcaac
    29581 tacttgatca tgctgagaag ataataagtc tgtaccaaaa tgaagataat ctttatcatc
    29641 gattcccaat agatgtagta atgtcggcaa aacatcgatt tctccaccgt attcatgatt
    29701 gactgtccct ttagtataac caggcatatg gatcatcaat ggcactcgtt gcatctgtgc
    29761 gttgtcaaat tcatcccatg tatctggatc tttaccaaga gcgcttgcta aatctttatt
    29821 atctgtattc gagatcccaa aatgatcccc gtaaatcaca aagattgaat tttggtaaat
    29881 accagatttt ttcaaatacg taaaaaattg ttccaatgat tgatccagat aatgtgccgt
    29941 tcgaacataa ttatctactg tgctattgcc agtattcaaa gatggaaagt cgaaattctt
    30001 gtcatccgta taataaggcg tgtggttggt tacggaaaga aatttcgtgt aaaacggctg
    30061 ttgtagatgc tcaagatatt gggcagattc tctgaataaa tctttatcta atatcccata
    30121 acctaacgtt tcgtcggatt catcaaaata agatcgatca aagaaattgt cataacctaa
    30181 gtttttatat acatgatctc tattccaaaa actagctaca ttcccatgga aaacagcgct
    30241 tgtatagcct tgcttttgtc caaggatcgc tggtgcggct tgaaagacat tgtctgaccc
    30301 aagttctgtg aataatgagc cttgaggtaa accaaatgtg ccagtttcca acatattttc
    30361 agcatcactt gtctttccct gtcctacttc atgaaagaaa ttgtcaaagc tgatcgttgc
    30421 ttgattctgg aaaatactat ttaaaaacgg tgtgacttct tgtccgtcta ccttcatatt
    30481 aatcaaaaac tgctggaaac tttctaaatg gagaacgatc acatttttcc ctttagcgat
    30541 cccaaatgtt tcgggatttg gctctgcata atgtttcttt gtataatcta aaacttcatc
    30601 aatcccatta ctgcttgcat gcgcgcgaac actgctagtc attcctgttt tgattccgtc
    30661 ataaatagta tacgcatcga ttcctagata tttgacaata tatgaacggt caaatgttcg
    30721 ctgcagcaat tgtggacggt tggcttcgct cagcgctaga tttcctgaaa aaataagccc
    30781 tgaaagacag gtaacagcga tagccatcgg tttgccgaca gggttgcttt tgataggtat
    30841 tttcttgacc ttcaaccaaa ccagtagccc aatgaacaca gcgatatcca gccagtagat
    30901 gacatcatgg ggcttcatca aagaaaagga gcttcctgac aatccttgcg acactttcga
    30961 aaagcctaat acagatttga ctgtaatgaa atctgtgaat tcccgataaa aaataatatt
    31021 caagtacaac aaaagcgtat ttaaaatatc aatgatcaaa agaatagcta acgctggctt
    31081 tggcttttta atatacaaag aaaggccaaa aaacaataag gtagtcgcaa ttgggttgat
    31141 ccacaagata aagtactgga tcgtaccttc cacacctaat gaaaaatctg catagtaggc
    31201 aatgatcgtc ttcagccaga aaaagaaaac agctgtcaaa acgattgcta aacgattttg
    31261 gcacaatttt ttaagttgtg tcacgtatat cacttctctc tattcacatt ttttcttatt
    31321 ttgctcctga aaaatgaaaa tttcctaaaa aagttgttat cttaagattt ttttattctt
    31381 tctaaagatt attccgtcca cttttcacag cataaaaccg ctgcgatgta agcgattaaa
    31441 ctaatgaaga gataaaagtc ttgtggtgac cgcttcactc cacggtattc aaaagccagt
    31501 ttctcggtat tcgtacaaaa aaagaggctg tgacaaaatt cttgtcgcag ctccttattt
    31561 cttgatgcag gacagctttt tcacaacttc ttccttaaac ggtgttctcc tgccaactcc
    31621 tcggccacaa gtcacaatat ttataaaaca tgagaagctg ttttttgaaa ttgttccttg
    31681 tggctcagag ttaaacggca ggctcaagac ctcttgatac acggtgttca atcagctgac
    31741 cctcggtatt agattatgtc aggacgctaa ttttacaacc tcttggtaaa cggtgttcaa
    31801 aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa tataaggagc tattttttga
    31861 aattgctcct tgtggctcag agttaaacgg caggctcaag acctcttgat acacggtgtt
    31921 caatcagctg accttcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttgcc
    31981 gtttgtttct ctaatactca ggtcaaaacg ctgatttcac aacctctgtt tttcattgtc
    32041 cataggattg atattgtcct tgtgatgact cattcgtaat aattgaggct tgattttctt
    32101 cacttcccgt tatcgtttgt ccactgccag aatctgaagg acttgttcct gtcgactcgt
    32161 ttttttgaga agagacagtt gttttcttga cttgttcatt tgtctttttc cattccgcta
    32221 aacgatcggc gaccactttt ttgtctactt tttcagaata agcggtgatc acttttccat
    32281 ttttcaaacg ggctactttg tcgccatcat gagtcaaaag caaaacggtc aaatgcttgt
    32341 ctttaaattg atattgtctg agagaaatcg cactaagtac gtcatcgata ttaataaatt
    32401 gattttcttc ttttcctttt ggcaaataaa tgtccaattc gcctttttcg taattggcag
    32461 ttattttata ctccgcccac caataagttg agaattcttt tttgaaatta tctactgatt
    32521 ctttgcttgc tacgattttt tctgtttctt tctgagcaag atctgctgtc atttttcctg
    32581 aaaatgctgc ggataaaata agcagtaaga aaaaaccgaa taaccaaaag agaagaaacg
    32641 gtcttttttt cctcatgctt tcctccactc ctatttaatt ttgactattc tattatatac
    32701 aaaaaatgaa atagaaagac aaaaaagttg aaaaagatcg taaataattg aacttctttt
    32761 tcacaaaatg gtaagaatgg agaatttcac gcatttgctc tcaactattc tttcttaagc
    32821 ctcaaatccg tttttatctg atacgtcttt gaaccaatga cccgattttt tgattgttct
    32881 ttttccatct tgtgctaagt ctactgaaat aaagccatat cgattcttat atgcattggt
    32941 ccacgaccag ttatccatac atgtccacat atgatagcct aaacaattgg caccttcttg
    33001 aatggcttga tggacatatt tcagatgaga ttggacaaat tcgatccgat aatcatcgtg
    33061 gatcatgcca tcttctttga taaatcgtgc ttcgttttct acacccatac cgttttcaga
    33121 aataaagcag tcgatatttc cataatgatc tcgtacattg atcaaaatat catagattcc
    33181 ttgttcatag atctcccatc cgcgataagg attcatttta cgaccaggca tttcataatt
    33241 gtcgaaataa tcctcaggca ttggaccgtt tttagtatcg attggtgttt cttttgcttt
    33301 tacccggcgt ggctggtaat aattgatacc taaaagatct actgtatttt ctttgataac
    33361 cgtcaaatcc tctgcatgga tcacaggcat gagatcaagt tcttttacta tctcaatcaa
    33421 ttcacttgga aactcgcctt ttacagctgg atcaaggaat gaacgattga agaacgcatc
    33481 tgctatttgc gctgctttaa catcttcagg gtttgattcg tctcttggat agcttggtgt
    33541 cagattcaag atgataccaa taacaccacc ttgctccatt tcatggtaag atttgatggc
    33601 taacgctgaa gccaacgctt catgatagcc aacttggatc gcttttttca tatctgactc
    33661 ttctggataa tgccatcgat ataaataacc tgcttcgact ggaacgattg gttcattatg
    33721 agtaaaccat ttttttactt tcgaaccaaa caattcaaaa caacttttgg catatttttc
    33781 ataggcttca accgtttctc ggttcaacca tccgcctttt tcttgcagat acatcggcat
    33841 atcgaaatga tacagattca caaagggttc gataccgttt tcaatcaact catcaaaata
    33901 acgatgatag aaacgaacgg cttcttcgtt gattgttcca gattcatcag gtatcaaacg
    33961 gctccattgg atagatgttc gaaatgaatt atgcccagtc tgtttcatca actgtacatc
    34021 ctcttgatac ttcgtataaa cttgcgaggt tttttcaggt cctacttgat caaagaactt
    34081 ttgcggttct tttttgtacc aataatcaaa aatgttctct ccttttccat cgcctgaaat
    34141 ccttccttct gtttgtgggc cacttgctgc agaaccccac caaaaatttg ctggaaattg
    34201 atattttttc atcaaagcac ctctttttct tttttagttc gttttagttg attccatgta
    34261 tgatattttc catcacaatc actttttcat tttcttcctc ttcttttttg attcgtttta
    34321 acagctgttc tgctgctcgt tccccgattt tatctgttgg tggcgtgatc gtgattttcg
    34381 gaaggatact cagatcgttt ttatctaaat aatcatagtg actgaacgca atatccttcc
    34441 cgattctgac accttcttga ttgaaacatt cgatggcacc aatcgacata ttgtaattgc
    34501 tgacaaaaat agcagttgcc cctttttcta ataatgcttt tgccccgtaa tagccgcttt
    34561 ttctagaata gtctcctgta aagacctcta attggatcga tggatcattt tggaccgctt
    34621 ctttggctcc tgctaaacgt tctcttgcta cgtagtctgt ttgagtggca gcaataaatc
    34681 cgattttttt atgtccttgt tcaccatgat gagcgatcac ttttgctaca ctttctcgat
    34741 cattcactaa tattgtatca atacctgctg gtccttctgg acaattcaat gcgataactg
    34801 gaatactcat ttttgtgagc atttcaaatc cagaccactc ttcttcggat aagaagacaa
    34861 tcagcccatc gatcacatgc ttcatcaggt attcaatttt ttgttcaatt ttttctggct
    34921 gatcggcaaa cccgcttaat aataagctat acccattttc ttcagctact ctttcgatcc
    34981 ctgagacaac ttccgaccca aaacggctgg aaatcccgtt tatcaaaagt ccaatagaga
    35041 agcttcgatt attcttcaac cctttagcaa tgatgttttc tttatagtcc aacgctgaaa
    35101 tcgcttctgc tatttttttc atgttctttt ccttcagttg ctgaccattc aagtaacggg
    35161 aaactgttcc cactgaaaca ccagccatct tagccacatc acggatcgat gccatacatt
    35221 ctcactcctg ccagttacat ttgatttctc tttcttgttg ttcgattatg atgaccgcat
    35281 tctttgcata gtgttgcttg tcatcaatat ccatataact aaagtcttca gtagagcaac
    35341 ttcctctcac tattaattat acttaaacaa gtatttgaac cggttcattt ttaatatagc
    35401 atttttttaa aagattgcaa cactttttta aaacgctttc tgaaagtggc ggattttttg
    35461 cctttttaaa attttattct aaaatttact ctatttgtgg gtgttttgtg cgtttttctc
    35521 ttccatccgt ttgaagtttt acattccaat ggtataattt ctttatccta tcaaatgggg
    35581 gaagtatttt gaagaaatta atcgtactat ttattttgtc tgcgggactt ttgacattga
    35641 ctggctgtga tttgtttggc ggctctgacc aatcctcgga cgaaacaacc gattcttctg
    35701 tcttaaaagc ggaaaaagca gataccacca acagtgtata tacgctaaaa gtacgtaaag
    35761 atatggattt atccgcggga cagttgacat ttacagataa aaaaatggaa tggacacgca
    35821 cgtatcagac acaaggaact gattcttctg aatcatctga agaaaaaaca gtgctgacag
    35881 atatcaagat cactaccaaa gatgacgaat atattatttc aggaaaagaa aataaaaaag
    35941 aaacaacagt catatttaca aaaatcggta attaccgttt gaaggatgag gatggcaaca
    36001 tctacagtct atagtgccgt ctgttggatc ttcttttgtt tctccccttc ttttctgcta
    36061 tagtaaagaa gcaagatgat tgtaaaggag acgaaatgga tgatcgatag tcctgattat
    36121 tgtacatgta ataacaacaa aaatatccat acagaattta atgaccaagg aagcttgagc
    36181 atatgcgaca actgtcacaa actagtcaaa gattcattta aaacatttga atcttatcct
    36241 gttcaaaatt atgtaaaaag tagaagaaga taaacaaatc caagcaaaac tcactatggc
    36301 tgatttttat gaacttcaaa ctcagcttct tcctttgatc aagcttcatc agtaaaacaa
    36361 acagtggatc atttcagttt acaaatcaaa gagtaaagat ttatttattt caaatatttg
    36421 aatataaaat aaccaaacga tagaaaggct tattctacat cacgtagtaa tttttctttc
    36481 ccgtcgtttg gttttttatt tttagcctaa ttcttgacct tttgcatgct ggttatccgt
    36541 aaaatatttt ccagcaggct ttccttccaa ttcagctaat ttttcttgga tctgacggat
    36601 cacctttttt gcatcttctg gattttctac aaaatcgaat ttatccccat ccacttgtat
    36661 tttaggacat acatcaaaat cttcatacca ttgattatag cgggtgttca acgtttcata
    36721 ataggtatat aacgtctcat cgctttctag ctgttcatag tcacgcccac gttttttgat
    36781 acgttctagc atcgtgtcca aagaaacttt gatatggacc atcaaatcag gccgtttttt
    36841 tggagcaaca tcatctaact cgtttagcat cgtatctaat aagctatcgt actgctgtac
    36901 ttcgatatcc gttacccgac ctaaatcagc gtttagatgg aacaacaggc tatcttcata
    36961 gatggatcgg tctagcacat tgtcatcgtt cactaaagca tttttcattg ctaaaaatcg
    37021 cttgttcaaa aagaagattt gcaataaaaa agcgtactgc tcaggatttg aataaaagag
    37081 cggcaatact tcgttatctt cgacattttc ataaaatggt ttagaatcaa tctcttttgc
    37141 aagcatatcc gtcaaactgc tcttacctgc accaatcgtt cctgaaatca aaatcactga
    37201 catacaaccc ttcctcttct ataagtactc gcctacgtaa gtttcaacag gcaatgtttc
    37261 aaaatcatat tcacgtacag cttggtctga ttttaaatac gtatggagac tttgtgtaat
    37321 catcaagttc gtaggtaccg tctcttcttg tacaatgatc atcggttttt tcaacatagt
    37381 tgccacacct aattcgtaag ccgttcctgg atcgattgtt tgatgctcaa agtcaaggat
    37441 agcaacaaca aattctgcat ttgtcagttc ttccatatct ttttcgtata cttcctgcgc
    37501 ccatccagcg ctgaatagtt cataattgct ttcttggtga tgacgtggag agtagaaact
    37561 tgtgactgtt ttgttttcct ccaacgcttt ttcgatacga cttactcgat cgatttgctc
    37621 ctctgaaaaa aatggtcctg ctaaatatat ttgactcatt attttgcctc cttcaataat
    37681 aactgatttt taatagatac tgctcgtaat tcattgacca gttccaatgg attgttggaa
    37741 aagattactt gatccaacca ttttttatcc ataaaatcct cttttgcaaa agtttctaat
    37801 aattgttgca agccgctgta ataaccgcca atattcaata ttccgattgg cttgtcgggc
    37861 gtcagaccga ttgctatcca agaaagaagt tgtgagaatt cctccaatgt acctagacct
    37921 ccagggaaaa cgaggacagc atctcccttt tcaaataata atacctttct ttcatcgatc
    37981 gtttctgtag gaataaatgt agtaacatct tttccaggca attcagtttc aaaaagccca
    38041 ttcggataga tacctgtgac ttttccgtta ttttccagta ctgttttact aatcactccc
    38101 ataatcccgg agtatgaacc accgtaaacc agttcgattt cttcttgtgc catgtatttt
    38161 cctaacgttt gcgcaataaa tttgtaactt tctttattac caaaacggga accgcaaaaa
    38221 acagtaagtt tcataaaaaa acactcccta actcaaaagt ctatcttcat tctacacgtg
    38281 tttttttaaa ataaaagact agattttgta gttttttagt ggtatatagg atagacgcca
    38341 ctatatatag tatttagaaa aaaatcattt ttcagccatt ttatagataa aacaaaaata
    38401 aataacgtgt tttcatcgat atgaaatgac ttacttatag aagatactgg gatttcgtcc
    38461 tatatgccaa taacgtgtat tattttaaac aacaaatact tgtttctttt attaacgaaa
    38521 aaatcatttt gttctcgtgt tatgtttcac tgataaatga tgtttctcta aataaaaaag
    38581 ccgacgaact aaacgatcaa aatttgatca tttttgttca tcagcttctc ctaaaaatct
    38641 ttttgttaac gataaatagc tggatttgta aaaatatcac ttgaatcgtc actgctggaa
    38701 gaaaattcag tgataaccgc acctgttgcg cctgcctgga actagtgttt gatattgggc
    38761 tcgaacgtgt actgatctct agcatgaaga ataatttctt tttttactgt ataataactt
    38821 tctttccttt ttggaatagt acaggaaagt ttttttgttt cctttccttc gacataaggt
    38881 ataccagacc ctttcgacaa cgaaaggttc tcatttttcc ttcatcgtca tttcaaggag
    38941 ggtgccgatg ctctggacag tttcgatcgc tcctaaacca aaatctgtat attcgattct
    39001 ttctaactct gattctttca taccattcct gtctcaaggt acatctattg tacttttttc
    39061 ttgtaatcca ttctgcctct cctaactaat tgtcaaatca tcccaagata tgtcttcttc
    39121 ctctttttct tcttggatga tttctgcatt cggatctact ggttttttca ataataaaat
    39181 ggttattcct gtgacaaaag aaccagctaa caatgcacca atgaatgccc atggtctggt
    39241 catcgtagcg actgcaaaca ttcccgatgc cggaatacgg ctatctgcgc cccaaaacat
    39301 gcttaatccg ccacctactg tcccaccgac tcccgttgct atgacattgc gtgccaagtc
    39361 gttcaatacg atagggaaac atccctctgt gatctgacag atccccatag gaaaagcggt
    39421 cttcaatgta tcgatttctt gacgattgag tatattttta cgaatgattt tccctaagaa
    39481 ataagcgatc gtcaagccga atggggttac catcgaagct aaaatcaata cagtgattgg
    39541 ttcatagatt ccttctgaaa atcgtgccac acccccttat ccagaataaa tggtaatcca
    39601 gaaacagccg tgtatctgcg gtaataagcc taaccaaacg aaaaatatga gaagctattt
    39661 ttgtttggct gttcttatta cttgatgcag ggcagctttt tcacaacctc tttttttatt
    39721 tatgtttcat aatcaatatg gtaaggatgc agctccgtat ggtagaccgc ctccgaatat
    39781 tcaatcattt ccccaatttg cgtagacgat ttccgtatcc gtttgatgcc gatctgtgcg
    39841 tctgttttca gaattccttg ttcttcttct gttaaaaata ctgccgagaa atcatcagaa
    39901 aatgagtcaa tagtatatcc ttttttcgtc agcaaacgat acaaagattc tttgccaaat
    39961 tcttctctgc tgaattgcat catttcttgt ggaagataat gaacaaaata aatataaggc
    40021 tgattgttca aataatataa gcgcttaaaa caaacagctt gctcaccgaa aaaggaaaac
    40081 aaaggatctt ccttctttat atggaccaat tgcaaatcca gcgtttcttt ccggacttca
    40141 tatcgggagt tctccaagat ttgcgtaaag cttgccgctt tggataatcg attgtaagga
    40201 cgattgctta gaaccgtcgt cccacgacca cttttctttg cgacatattc atctgatgcc
    40261 agcagttcga ttgcctttcg gacagtgatt ttgctgactt gaaacagttc ttctaattca
    40321 ctttctgtcg gcaacatcga accgactgga tattttcctg agaaaatatc ttctttaatt
    40381 ttatctgcga cttccaagta tagtacacgt gtccttttca taacgtcacc ctttgcttta
    40441 ctattactaa aagaatagca cataaaaaat aaaaaaagaa cacaaatcac atgtgagaag
    40501 tgtgttcttt ttcattcagc tgctttaatt acaagtttac gccatttgag gcaattactt
    40561 ctttatacca atcaaaagat tttttctttg atcgattcag tgtcccattt ccttcatcgt
    40621 ctagatccac atagataaag ccgtaacgtt tactcatttc tcctgttgat gcactgacaa
    40681 gatcgataca tccccaaggc gtatagccca tcaaatctac accgtctgct actgcattag
    40741 ccatcgcttc gatatgacga cgtaaataat cgatccgata atcatcaata atcgtgccat
    40801 cctcttccat atgatcaatg gctccaagac cgttttcaac aatgaataat ggtttctggt
    40861 aacgattgta taattcattc aatgcgattc tcagcccttc tggatcgatc tgccagcccc
    40921 attcacttgc ctcaagaaac ggatttttta ctcctcccag caagttccct tctgaagttg
    40981 ctgcttgtgg atcagtctca ttgattgccg tagacatata ataactaaat ccgatgtagt
    41041 ccactggata ttcttttagc aactggaagt ctttttccgt ttgctctaat ttttccggat
    41101 cgactccata ttttttatgg gtacgcgctg tgtatgcagg gtattctcca cgtacttgga
    41161 catccgtgca gaaaaaattg aattcttggt tttggatcat tgttgctgcc tgattgactg
    41221 gattggcatc gatgctgtaa gttgttgcat agataatcat gcagccgatt tggatatccg
    41281 aacgcaattc gtggccgatt tttacggctt tgctacttgc aacgaattga ttatgccatg
    41341 cttggaaaat attttggtac tctcctgctc cattgctttt tactaatcct tgacttaaag
    41401 ctggaaagtg aaaggccgaa ttgatttcat tgaaagtcat ccaatatttg actttactgc
    41461 tgtatctctc taatactgtc tgagcaaatc gttcatagaa gtcgatcagt tttcgatttt
    41521 tccagccacc gtattctttt gccaaatgaa gaggcatttc gtaatgagaa atcgtgatca
    41581 caggttcaat atcatatttt aagcactcat caatcagctg atcataaaat tctaatcccg
    41641 cttcgttagg agtaccttcg tctccatttg gaaaaattcg tgtccaggca atcgaaaaac
    41701 gataacattt gaatcccatt tccgcaaaca aggcaagatc ttctttgaaa cgatcgtaat
    41761 gatcgattcc tcgatgattc gggtaaatat atttttcttg atcaatcgtc cagtcaaatt
    41821 cttcactgcc gataatcgca aagcgctgct tacctccagg catagcatct gcaacggata
    41881 gtccttttcc gtctcgatca taagcccctt ctaattgatt tggcagcagt tgctccaccc
    41941 cataaaaatt tttctgaaaa acgtgattgc atcctgattt cctccttatt ttacaattgc
    42001 cagaacatct tcgtttgcaa taatctcttt ttggtctaat tcaagtatat cggcaaatgc
    42061 atcagtattc gtaatgacaa ctggagtgat tacgctatag ccagctgctc taattgcttc
    42121 gatgtcaaaa cgaatcaata aatctcctgc tttgacgaga tccccttgtt tgactaagat
    42181 ctcaaagcca ttcccattta attctactgt atccatcccg atatgaatca aaatctccgc
    42241 tccgtcagtc gttgtgatcc ctacagcatg tccagttgga aaaatagtcg tgatctctcc
    42301 gtctgcaggt gcaactagtt ctcctacttc tggatcaata gcgatacctt ttcccatcac
    42361 gccagaagaa aaaacttggt ctggtacttc actcaatggc agtactttac ccgttaaagg
    42421 actttttaag gtatgccgtg ctgaagtaat cggttctcct gcattcgcat gtttgttttc
    42481 taattgattg tcttgtgttt gttcgtcaaa tcctaaaatc aatgtaccta cgaatgcaag
    42541 aacaaaagca attcctgtag ctattactgc tactgtcaca tttgactcta caccatccac
    42601 cgtattaatg aaagtcggaa tcgtcaataa gctgaccaaa ctgctggaga aagcttttac
    42661 tccggaaaat ccgataatag ctccaccaat acctccagaa atacaagcgg caatgaatgg
    42721 tttcttcaaa ggtaatgtca caccatacac agttggttca gtgattccaa ataacgaagt
    42781 cactgtagaa gaaatcccta atgctcgaag tttcgtatct tttgttcgca atgctacagc
    42841 taaagcagcc ccaccttgtg ccaagatggc tggaagaagc attggcatca atacatcaaa
    42901 cccatattgt tcgatattca aaaacatgat cggtacgaat ccccaatgca tgccgaacat
    42961 cacgaatact tgccataaac ttcccatgat tagacctgct acgattggac taaatccata
    43021 aatcgagttg aagagaccgc ctaaagcatt accggcaacc gtcccgattg gtcctaaagc
    43081 aagaaatgtt aatggaacca tcactactaa aacgacaagc ggaactaaaa tcatttgcag
    43141 aaattgcgga atcacttttt ttacgaatgg ctctaattta ctttgaaccc atacggctag
    43201 aatgattggt atgacagaag aagtataacc agatggtgaa agaatcactg ggatcccaaa
    43261 gaaactgata gcttctcccg caccagcaac tgctgtgata gctggataca caagtgccat
    43321 agcaattgct actgctaaaa actggttggc tttgaacttt ttagctgctg taaaagcaag
    43381 catgacaggt aagaaggtaa agacaccatc tgctgcagca aacaatattt gataagtacc
    43441 gctatcagct gtcagccagc ccatcaccgt tgccaatgat aagaatcctt tcaatacccc
    43501 ggcggctgcc atcgccccta gaaaaggtgt aaatatacta gagataatat cgatcagacg
    43561 attcaaaata tttttggact cttcttttcc atcctccgaa actgcaagac cttcctcatc
    43621 cacgatcgat tgataaacat ctgctacatt actgccaatc actacttgat actgaccgcc
    43681 actttgtacg acttggatca cgtccgggtc ttcttttaac ttttgcgtat ctgctaaaga
    43741 ttcatctttt aatctgaatc gcaaccgcgt agcacaatgt actaaacttt tgacattctt
    43801 ttgtcctcct accgcttccc atacacgacg accaactgct tgattatcca ttgttttttc
    43861 cttctttctt ttttatttaa aaaataaaaa cccagattga aaaaaacagt cacaaacatt
    43921 cgtgaaccta ttttttcaat ccaggttttg cctgccatac agtaacaatc cttatgattg
    43981 gatgcttttt ttctcactta ctactctttg aatatgaatc gttaaataaa tctgctctgc
    44041 gatccccatt tcctgttttt gcgtttgtcg aagatactca ttgatccgtt cgacacaatg
    44101 aaacgctttt ggatactttc cttgaactaa tgcgtagaga aattcatctt gctcgtcaga
    44161 tccttcccct ttgagataac gctgggcaaa aaactgcaga tgagtaatga tacgctgata
    44221 attcaaagat tcttcatcga acacgatgcc gaagaattta ctgatgatac tcagtatatc
    44281 tcttaccatt gtggtagcgg tcatcgtcac ttgcatgtct tcgtttcctt gttggctatt
    44341 cacaaaatga aaggcaataa aaccggcttc atcctcagaa agttccacac ccgtcttttg
    44401 tttcacgaac aaaagagcct ctttcgctgc ttgatattct ttcggataaa atttttttgt
    44461 ttcaaataat aatggattag gcagttggat attttctttt gctcgtgtga tcgcataatg
    44521 aagatgatcg gtcaatgtca gataaatatt aggcgaaaat tcaactttta gtttatcttc
    44581 agccagttcg actacttgtt tgacccattc gatctcctct gccggaatat cagccatcag
    44641 ctgctggaat tggtttttag caaaagaatc tcttaaaaca aattcttttt ggaccgcttc
    44701 tggcgaaatc tcgtccccaa tctttttttg aaaagctagt ccttttccca tacaaacgac
    44761 ttcttccccg aatccatttt tcgaaatgac gacattgtta ttcaaaattt tatcaatttt
    44821 cattttgctt cgccctctca agaaaaataa aaaaacctag aattcgccta ctgtttctta
    44881 gctgaattct ggttttgcct gccgtacagt aacaatccat ttcttattca aaatcatcat
    44941 accatcgaat ttttatttat gcaagccttt tcatataaaa aataaaaaaa ctggaacaca
    45001 agtctatcta gaaaacaacc aaatcagaga tgcacctctt tgcgtttctc tttttttcag
    45061 caaattggtt tttccttatc gattcatatc ctttggctgt ttttaatctg taacgactta
    45121 tgtcccagta tcctaaaatg tttttgtctc tagattgaaa gcagatctca tatcatctgt
    45181 cgcatctttt atcgatgtaa cactttccag ttctccttgt acaactagtc gcgtgacggc
    45241 gtccatgtcg ccacaagtaa ttagtgtgac aatttctttt ccctcgactt cattcagtaa
    45301 ttgggtatct gtaggagcaa ctttttcttt aaaaaatgtt ttgtatgtat acacattttc
    45361 taaatctgtc aaatagatct tggcacccat ctctaaattt tctagcggtg taaacagcaa
    45421 ttcagggttc gttgcacgat ggcttgctaa ggcataattt ccttttccca tttcttgatc
    45481 tggagataat gttcctgctc cgtaaagtag tgacgtatta tccaagcctt tgaaaaccgg
    45541 taaattgatt gaaactgaag ggactgcgat actagcaatc actggtagat ctgttcctct
    45601 catttgcgac ctcatgaccc cttctgtcgt cattggttct accgcatcaa agtcaaaact
    45661 gacatcttta tcgttattct ttttcagatc ttcttttgtt acattcgcta tagcatattt
    45721 atctcctgtc tctctgacaa aataatcctt gatctgttca ttgaaaatca aagctagtcc
    45781 tactaataac aagaggaaca agaggatatt gactagccat ctttttgctt ttcctttttt
    45841 tttcattttc tctccctcca gactcatttt actgtttttc ttttgctctt tccaacagcc
    45901 gaatgacaca tttctacaac tctgtgtatt tttttcgtac acctgcgctt ttttctacag
    45961 gatatttcaa attatttttg atcaattttt cttcaatgat ctcatctaga tccaggtgaa
    46021 ggttgtctgc catcatgtat gaatagatca atacatctgc cagttcttct ttgattcgtt
    46081 ctaattgtgt ttcttttact tcttccggcg ttttccattg aaaaagttct aacaattcag
    46141 atgcttccaa agaaatagag atagccaaat ctttttcgtt atgaaattgt cgccaattac
    46201 gttcatctct aaactggttg actttttcca tactattcat ttgcttgcct tcttccaatc
    46261 acttcttact tgattaatca taccattttt ttacataaaa caaagtataa atttctaatt
    46321 atctttttat attttcttaa atgctcgtaa agccttattc tatgtgcttt cgagtatttt
    46381 tactgtagga agatacttca cgtttctttg catatttcct catgtcttag ctgtcagaag
    46441 tggtaaataa gtagtaaatt catttgtact actaagcaac aagacgctcc tgttgcttct
    46501 ctttattcaa gcgtttcatt tctgccattg cagaatcgaa tgttgcatgt gcgtaatagt
    46561 tcagcgtcat ggctatatta gcatgtccca taatgtactg taatgccttt ggattcattc
    46621 ctgcatttgc atagttggta cagaatgtat gtcgcaaact atgtggagtg atgtgtggca
    46681 atttatcctc gttatactta ttgtatttct taacaagacc tttcatcatg ccgttgtaat
    46741 cacttgccac ttttggatag ttctttctat taagaaagag gaaatcacta tatccatcaa
    46801 tctcaacacg cttatcattc tttcgattcg ctaacactcg cttaaatgct tgataggctt
    46861 cttcaaccat aggaacttga cgttcgccac ttttggtctt tggtgtttca atgtagtacc
    46921 caatttcagt atctctcaat agctgatggt ctatattgac aagacgattc tcaaaatcta
    46981 aatctggaag tgtcaaacca ccaaactctg aaatacgaag acctgttttt aagagtatca
    47041 gaatttcatc ataatttttg ctgtaggttt tatcagcttt tgcaaaggct aacagttttt
    47101 cttcctgttc ttctgttagt acggtcttag ggacagtatc atcatcaaga actgctttca
    47161 gttgaaagtc aaatggattc ttccgaacac aatcatcttg tatagcaata tagaatgaag
    47221 cctttaaaga acgtttgtag ttattgatgg tttgataagc ataaccattt tcactcattc
    47281 taatagccca ttctttagcg tctgatggct taatactgtc aatgcttctt acacctaact
    47341 tgtctttctt caaaatatcc ataagatatt tgcgtccagt ttcagtgttt tttctaacct
    47401 ttggtctttg agcgttctgt tttgcgtaaa gctggcagag tgtcattttc tttcctacaa
    47461 catcaatacc atcatgaatg tctttctgta actctgcgat tttctctcta agtgagatac
    47521 aatcacgctt tcctgctggt actcggtctg tagccacaag tttccacgag taaacaaatt
    47581 gcggttctcc aaatgaatct atatatttgt ataagtatct tccgtctttt cgttggctct
    47641 ctccagtctt taagattcga cctctattgt cacgtctttt ttctgacatg gcatttgctc
    47701 ctttccttta tggaaagagc cttgatacga cttaatacta ttttatcata tacaagaccc
    47761 tttggcgacg ctagattgcg tccaatgtat ctataatttt ttcaaattgt tttcgtttaa
    47821 tctgaatacg attgccattc ataatcagcc aatttgcatt tttattttcc tctgccaagc
    47881 gtcgtagctt gttttcgcca atacgaaaat attttgacgc ttcttcaatg gttagggtat
    47941 aacgttccca aataggaatg tcagtctgct tcataaaatc ctcctttcca aatcacttat
    48001 ttggatttca taaaagttgt tttaccagca atcgaacagc tttagcaaag ctcacgggag
    48061 ttccacccct gcatggttct catgtagcca tactcattgc ctgcgacggt tttatcacgc
    48121 tcggactatt gactgtatgg gagtatcatt atcacgataa gaatgtcgtt gcaggcaatc
    48181 ctgctaaaga ttgcttctcg gatcactaac atgaatcgct cgctatcttt ataagatagg
    48241 tcatggcggt tagttccgtt ggctcttttc ttatcgaaac gtattcgatt acttttattc
    48301 agttttcaaa gaacaatggc tcgttagcct atcaaaacac attgaaagct caatatgctt
    48361 tggtggaata acaaacctcc ctgttcggga agcgtggaat ggtttagcac gcttccacga
    48421 aaggagagag gatattactt aatttcaaat gacaaaatct ttgtaatcag tctggtttcc
    48481 attcttccac gtaagacttc atcaacgacc atactttgat tgccatattc atctttcata
    48541 agtcgtaggg aacgcttcgt tatgtaccct ctgtaatgat gtagaatctg gttaatcgct
    48601 tcggtatcgc catctgttgc ctttacaatg agaggaaagg gaatcatagg atattgtgtt
    48661 ttcattcttc aaattcctcc ataaactttt taattaaggc tagtccactg gttctatgcc
    48721 gatagacagt agaacggttc aatttcaaca ggtctgcaat ttctgaatcg ctcatgtcca
    48781 taaagtaaaa cagcagtaga atttcacgtt tcttgtctgg caactcacgt aatgcttcac
    48841 tcaacaaatc attttcaacg cctactgata acccattgag tgtaaaaatc tgaaagtcag
    48901 ttgaatagtt atctgttgtc gcaaactggc taacaagata atcgccaaca tccgaaaagg
    48961 acacctcacg ctttgcaatc cttgaaagat aaagcatata attctttcgc tcgtcttcca
    49021 tagcacgttt acagatatag tcaaactgat tttctattgt ggtctgaaaa gaagatggtt
    49081 tcatgtttct cacccccttt ctgtctagga aaggaagtga gccttgctcg tttatctcct
    49141 ttcactctta gtcccaatgt gaaaggggga tttgttgcat tactgataaa taaactttgt
    49201 aaaaaagttc tgaatagcca aaaaagcata taaacagatt tatttctctg tttacatgct
    49261 tctgttattc tatctatatg atttataaaa ccacattggt ggacgtactt atctattgca
    49321 gatagacgac tttttttgac aagaacccaa tgtaaggaaa tttattgtat atgatgtact
    49381 tcatggcgac gttgacctcc aacaaaccgc catttggaag taatatacaa tattttaaca
    49441 gcgtaaatag cactaccata taacggtttt ttttattggc gtttagtagt gctttttatt
    49501 aa
//
LOCUS       DACDEG010000013        44591 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.013, whole genome shotgun sequence.
ACCESSION   DACDEG010000013 DACDEG010000000
VERSION     DACDEG010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.013"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            32..991
                     /locus_tag="IV142_000809"
     CDS             32..991
                     /locus_tag="IV142_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8484484.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            1161..1841
                     /locus_tag="IV142_000810"
     CDS             1161..1841
                     /locus_tag="IV142_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8484485.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            1931..2965
                     /locus_tag="IV142_000811"
     CDS             1931..2965
                     /locus_tag="IV142_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP8484486.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            2979..3314
                     /locus_tag="IV142_000812"
     CDS             2979..3314
                     /locus_tag="IV142_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484487.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            3298..5778
                     /locus_tag="IV142_000813"
     CDS             3298..5778
                     /locus_tag="IV142_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484488.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            5834..7393
                     /locus_tag="IV142_000814"
     CDS             5834..7393
                     /locus_tag="IV142_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8484489.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            7390..8754
                     /locus_tag="IV142_000815"
     CDS             7390..8754
                     /locus_tag="IV142_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8484490.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            8925..9995
                     /locus_tag="IV142_000816"
     CDS             8925..9995
                     /locus_tag="IV142_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8484491.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            10010..10678
                     /locus_tag="IV142_000817"
     CDS             10010..10678
                     /locus_tag="IV142_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8484492.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            10698..11276
                     /locus_tag="IV142_000818"
     CDS             10698..11276
                     /locus_tag="IV142_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8484493.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            11677..12588
                     /locus_tag="IV142_000819"
     CDS             11677..12588
                     /locus_tag="IV142_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8484494.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(12966..13883)
                     /locus_tag="IV142_000820"
     CDS             complement(12966..13883)
                     /locus_tag="IV142_000820"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP8484495.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            14085..14522
                     /gene="zapA"
                     /locus_tag="IV142_000821"
     CDS             14085..14522
                     /gene="zapA"
                     /locus_tag="IV142_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP8484496.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            14580..15128
                     /locus_tag="IV142_000822"
     CDS             14580..15128
                     /locus_tag="IV142_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAP8484497.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            15243..17603
                     /locus_tag="IV142_000823"
     CDS             15243..17603
                     /locus_tag="IV142_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP8484498.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            17690..18004
                     /gene="trxA"
                     /locus_tag="IV142_000824"
     CDS             17690..18004
                     /gene="trxA"
                     /locus_tag="IV142_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP8484499.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            18186..19964
                     /gene="uvrC"
                     /locus_tag="IV142_000825"
     CDS             18186..19964
                     /gene="uvrC"
                     /locus_tag="IV142_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP8484500.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            20109..21008
                     /locus_tag="IV142_000826"
     CDS             20109..21008
                     /locus_tag="IV142_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8484501.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            21001..22263
                     /locus_tag="IV142_000827"
     CDS             21001..22263
                     /locus_tag="IV142_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8484502.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            22424..24322
                     /locus_tag="IV142_000828"
     CDS             22424..24322
                     /locus_tag="IV142_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP8484503.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(24652..25371)
                     /locus_tag="IV142_000829"
     CDS             complement(24652..25371)
                     /locus_tag="IV142_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP8484504.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            25708..26040
                     /locus_tag="IV142_000830"
     CDS             25708..26040
                     /locus_tag="IV142_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484505.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            26027..28030
                     /locus_tag="IV142_000831"
     CDS             26027..28030
                     /locus_tag="IV142_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP8484506.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            28049..28519
                     /locus_tag="IV142_000832"
     CDS             28049..28519
                     /locus_tag="IV142_000832"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP8484507.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            28540..29124
                     /locus_tag="IV142_000833"
     CDS             28540..29124
                     /locus_tag="IV142_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP8484508.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            29139..30125
                     /locus_tag="IV142_000834"
     CDS             29139..30125
                     /locus_tag="IV142_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP8484509.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            30115..30426
                     /locus_tag="IV142_000835"
     CDS             30115..30426
                     /locus_tag="IV142_000835"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP8484510.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            30448..32229
                     /locus_tag="IV142_000836"
     CDS             30448..32229
                     /locus_tag="IV142_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP8484511.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            32222..33598
                     /locus_tag="IV142_000837"
     CDS             32222..33598
                     /locus_tag="IV142_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP8484512.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            33605..34240
                     /locus_tag="IV142_000838"
     CDS             33605..34240
                     /locus_tag="IV142_000838"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP8484513.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            34270..35625
                     /locus_tag="IV142_000839"
     CDS             34270..35625
                     /locus_tag="IV142_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP8484514.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(35817..37211)
                     /locus_tag="IV142_000840"
     CDS             complement(35817..37211)
                     /locus_tag="IV142_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP8484515.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRSNQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            37303..38274
                     /locus_tag="IV142_000841"
     CDS             37303..38274
                     /locus_tag="IV142_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP8484516.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            38271..39053
                     /locus_tag="IV142_000842"
     CDS             38271..39053
                     /locus_tag="IV142_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP8484517.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(39222..39593)
                     /locus_tag="IV142_000843"
     CDS             complement(39222..39593)
                     /locus_tag="IV142_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP8484518.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            39727..40170
                     /locus_tag="IV142_000844"
     CDS             39727..40170
                     /locus_tag="IV142_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP8484519.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(40213..41022)
                     /gene="yidA"
                     /locus_tag="IV142_000845"
     CDS             complement(40213..41022)
                     /gene="yidA"
                     /locus_tag="IV142_000845"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP8484520.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(41041..42408)
                     /locus_tag="IV142_000846"
     CDS             complement(41041..42408)
                     /locus_tag="IV142_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8484521.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(42392..43222)
                     /locus_tag="IV142_000847"
     CDS             complement(42392..43222)
                     /locus_tag="IV142_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP8484522.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            43385..43822
                     /locus_tag="IV142_000848"
     CDS             43385..43822
                     /locus_tag="IV142_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP8484523.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            43898..44518
                     /locus_tag="IV142_000849"
     CDS             43898..44518
                     /locus_tag="IV142_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP8484524.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        14639 a         7668 c         9441 g        12843 t
ORIGIN      
        1 ccgacgaaag aattataagg agtgagtaca aatgacctat aaccatctta caccaactga
       61 actcgtaatg atagaagctt atttcaatca atcacaacct gtttcaaaag tagccaatct
      121 gttacaacgt tctcgacaaa ctatttataa ggtctatcgt tttcttaaat ctggtggaag
      181 cgcaatgagc tactataaac aatataagaa aaacaaacgt aactgcggaa gacatccgat
      241 tgttttacct gatgagcaac aggaatacat tcaaaaaaag gttgttcaag ggtgggctcc
      301 tgatgtcctt attggtcgag cagagtttcc tattgcttgt tcggtacgca cactgtatcg
      361 aatgtttaaa gataaaacct ttgacactca cgatttacca atgaagggaa aaagaaaacc
      421 aaacggtcat aaagaaaaaa gaggaaaaca agggtttcgc cggtcaatcc gagacagaaa
      481 gacagactat acccagtttg atcatgaatt tggtcacctg gaaggtgaca ctatcgtggg
      541 tggaaaacat aaaagcgcgg tcatcacatt agttgaacgg ctatctaaag tcatcattac
      601 cttgaaaccc gaaggcagac aggctaaaga cattgaaaat aggctgaatc aatggtttcg
      661 ttgtgtccca aaaaacttat ttaaatccat cacatttgat tgtggaaaag aattctcaaa
      721 ttggaaaaag attagtaata ccaacgatat ttcaatttat tttgcggatc caggaacacc
      781 ttctcaacga ggattgaatg aacattcaaa tggcttatta cgtaaagatg gtttgtataa
      841 agctatggat tttaatggtg tatctgaaac ctttatccaa tcggtggcat cgaaacgaaa
      901 tcatattcct agaaaatcat tacactatcg gacaccaatg gaagtatttt tgagttacgt
      961 cacaaaagat gatttgtcta acttaatttg acaaacgaaa cttaataaaa aaggaagaat
     1021 attcatgtaa acgattttat tgtttttttc tcttttatac aaaaagggtc ataatttcaa
     1081 gtaagcggat gaattcatga ggattttttg catactgtct ctactatact taggtcacta
     1141 agaaaaagga gtgatttatt atgaaaaaga acatgattgg gacattatta ctttcaacac
     1201 ttcttctagg cggcttggca acaccagctt ttgctgaggg tcaagcgact tcaaaaggag
     1261 atattacctt tactgaacca acaaataccg tggagcctct taaccctact gatccttcaa
     1321 aaccagtaga gccagcagat ccggaaaatc cagcaactgg ccagacaggt cctttaacac
     1381 tggatgtagt tcccgaattg ccatttggta cacacgagat tgaaagcgga acaaaaacgt
     1441 atcaagtaga cgcttcaaaa aacgatacac cttatctcca agtatcagat cgtcgaggtg
     1501 taggtgcaga tggccaagca caagggtgga acgtgacagt ttcggtttca gattttgtaa
     1561 acggttcaca agttttgcaa ggcgctgaat tagattttgg aacatccact gttaaaagta
     1621 cgtcagataa cgaatccact ggaccaacta gtcaaactgt aactgggttg agtaaagcaa
     1681 gtgcagcgac accaatattt acagcagcaa aagatcaagg tctgggaaca tggctttctg
     1741 tttatgatcc agcaaatatc acactgaaag tgccgaaagc agcagctgga acattcacgg
     1801 ctgatttgac ttggaacctc gtggctggcc cagtagcata gagtatgaaa taaattcaac
     1861 aaactgcggt gaaaatacta taggtgaagg attttttacc gcagttgttg ttgaaaggat
     1921 gtggtggaca atgaatcaaa gtaaacgact gtttgtttcg tttttttcta ttttaagtct
     1981 atttttcatt tttccgtcaa tcagccaagc aaaggattca gcaggagact ttggaatcaa
     2041 accggtattt ccagagaacc aaatagataa agcaatcggt tattttgatt tattagtggc
     2101 accggaacaa aatcagacac tagaagtaat tatcagcaat tcttcagatg aagaacgaac
     2161 atttgaggtc tctgtcaatc cggcggtaac gagtgatggt ggaacgatcg attatagtca
     2221 aaaaaaccca acgttagatg agacattgcc gtttgatgtc cgtgacgttt tgctcattgc
     2281 gaaaaaggaa atcaatgtat cagcacatgc agaaacaact gtacctatag aagtgaaaat
     2341 cccagcaaaa agctttaagg gacgtgtatt agcgggtatt catgtttcac ctaaagaaga
     2401 agctgaaaca gaaaatgcta cagaaggagc acaaatcaaa aatcgtattg cttataatct
     2461 cgcagtcgtt ttacaagaaa gtcaagagac gatagaaccg gatctgaaac ttttatcagg
     2521 agatttggac gaggtcaacg caaagccaac tgttcagctt cgtttccaaa atcctcagcc
     2581 taggattatc agcaatttga tatttacgag caagatcttt tatgaaaatc agctttatat
     2641 tgaaaatacg tctaatgcat ttttagttgc accaaattcg aattttcact tgaatcttga
     2701 tttagctggt gataaagcaa aagccggcga ttaccgcgca gaaatcatag caaaatcagg
     2761 cgacagtaat gaatggcgat ttacacaaaa ctttacgatc aaaaaagaaa aagcacaaaa
     2821 agtaaatgaa aattccgttt ttgcagtaca agaacaatct ttcccatggc tttatgcagg
     2881 attggttgct gcagttctag tgttatggtt gattttgctg tttttgtggg tccacaagaa
     2941 aaagaaaaaa ggagaaacaa catgatgagg tgatttcgtt gaaaacatcg atcttattgg
     3001 gagggattat tgcaagtttg ctgttcttca gctggcaaga gccagtccaa gcagatacca
     3061 aaagttcagg tacgattaca ttttatgatc taagtccaat tccgcatcca agtggttcag
     3121 tcaatggcgc atcaaaacag cagacacagc taccgattta taaagaatct aatcaacatg
     3181 gcgtattccc ggagacgaat gaacaaaaga tccgttttct ttatttgatc attggtatag
     3241 aattatggct tcttgcgtgg ttgttgttat ggagaagaaa ggaaaaggag gatgaacatg
     3301 ttgctacaaa ataaattttc attttttcgc tccttctttt ttacttcgct ttttgtttta
     3361 agtatattta tcttttcacc ttcggcagaa gctgttggag caacgcctcc tgccatttct
     3421 tatgatccat acacgtatga aaatatcgta cctccaaatg gtggacgtag tgcagtcaat
     3481 ggcgggaagc ttgtccaact aactggggct aaccaattgc ctaaattgac tgctacttat
     3541 ttgaacgatg gttcaaatga tccttggaaa ggtggcacgg tccgttttca ttgggaaagc
     3601 catgtgaaac taacaaatgt acttgatttg gtgaatatca ctgcacaagg atttgcattt
     3661 gtggcgatca tcaatttgcc ggaacatgta tcaagcagtg atgtattaaa agcaattgac
     3721 tggaacaacg cctacttgta tattgccggt gcccccatca aggttactag cggaagttta
     3781 ttatcagcag gaagccatac cttgcgtttt gggatggggt cgtgggatac aaatactcta
     3841 ttgacagata ttattgctat tattacgcga ggcgaattca acgtggacga tatcccgctt
     3901 caatttgatg ttacagctaa tgtcgcgaag atgacagaaa acggagataa atatgatacg
     3961 agtccaaaca aaattttgac caagggtaaa ttacaacctt ctatgtctaa aaaggccgtt
     4021 ttttctgttg atttttatga ttcgaataat attatccaag atcgtatgtt caattcgatt
     4081 ttagctccag ctgcaggtag attttatcct aaattaactg gcggaaactt gaatccagga
     4141 gcatatgcat tttctgcaag tgcagagata aatacgtgga atagttacat ctcaccaagt
     4201 gattcaacag gaaaatataa tacatttgct gataccacag aatacgtgga tggaaataac
     4261 cagaaccttc caggctctac caatagtcgg acattgacga tggggctgac tgaaggacgt
     4321 tttgatcagt tgccatttag tcgctttgat cgcgtagtca actattttac aggaaaaaac
     4381 gttacacaag gagcaatact gactcatact ccagcaagca caagtggatt gaatcagaca
     4441 gccaatatcg tgtacagtgg gaaagatgcg caaggcggat tgctttctcc tgttgcttta
     4501 aaggtattgg agcagtacaa gctagacgga acggttttac caacaaactt aacagccgat
     4561 agtcagtggg gaaagctcct ttcttctgca tcgtaccaag tcacggccaa ttggtcagca
     4621 actgaactaa aaaagggttc gatccattac aggctttatt cgaaaggaac ccaaaaatta
     4681 gcgacaggat ggacagatca acttttccaa acgatccaat caaataacgg gggtgtaaat
     4741 acagctactt ctacactacc tgcattggct tcagggcaat attattttga ttatcgcttg
     4801 gtggacgatg tacttactaa catgtatccg tcttttgcgt acaaatggca gtctgaacaa
     4861 gctggaatca ccactcttcc tcaaataaca gtggctaatt ttccatctgt gagtgcagat
     4921 agtcagctaa aaaaccttag cagacaacct gagacaggag atcccctgtc tgctctatca
     4981 ggagatacga ttcaagaaaa tgtgacattc acgttaacaa aatctgggga tgcgataacc
     5041 gacaaaaaaa tcacgatctc acttcctgaa aatactactt atatcaatgg ttccttaaaa
     5101 ttaaatggta caactatcgc agataccggc attcaacaag gagtttctgt cccagcagat
     5161 ctattgagca aaataggaga taccattcat cttacttaca actatcaatt aaatactgta
     5221 gatacttctt tacaatccgt cagcatactg acaaaggcag ctgtactttc cagcaatatt
     5281 acattagcag atggtgctaa gcttcctaac ccagttgtcc aaacatctgc taaaacaatt
     5341 cttgtcccca aacaagaatt aacattagtt aatgtaccag atgattttac atttggtaac
     5401 gatctcccca aaccgttgaa gacaagttat tatgaatcaa aaggcgattt ttcttttgat
     5461 gtgcgggata ctcggcttcc aagcacgtct ccttggcaac tgacaggtac attgacttct
     5521 ttatttaaga atgatcaagg tcaagaatta agcggaacaa aattatactt caatcattct
     5581 ggatcaaagc aattgattca gcaaggtcaa aacacgttga tttatgagag tgatggaaca
     5641 gctaaaggag aggttctcgt tgactttccc gataccgatg gtttactctt agaggtaaat
     5701 agcagtacga atgctcaacc tggcgcaacg tatcaaggca tggtcacatg ggaactgaca
     5761 gctgggccaa ccagctaagt gattagaatt tgtatggatc tgtgataact gtcacagatc
     5821 ctctttttca aggatgagaa ggaaacttct gctgttaaaa tcagaaatgc ttgaaggagt
     5881 ggacttggaa cttttcagtt tgcttgaaaa aaatgaacaa cggcaaatca aacttttcca
     5941 acttttatta gaggaaaaag gagaggtttc cgttaataac gtacttaccc gcttatcagt
     6001 tgatcgggct accttgaaag aagatctgtt ttatttacaa gctaacttgg aaaatatcaa
     6061 acaattggaa attgttatcc aagaagggaa tattttatta atcagacatg gaaatatagc
     6121 agcagtcgat gtttattatc attgttactt gcgctattct acaaaatatc agatacttct
     6181 ttatctgttg gaaaatggct catttgatcg gcagaaactt gtcatggaac ttaatatcag
     6241 ttcggctact ttgtctcgca gagtgaagga attaaatgat tctttaaatg agtttcatct
     6301 gaaaataaaa agcgggcggc tgaccggtcc agaaagtcaa attcgttatt tttatttcca
     6361 attattttgg tttggacgtc cctatcttga aaacaaaaaa gaatacagtg atttatcagg
     6421 aaacactttt ttagatctgc tgcaaaaaga gtttagtttt gctattacag aagaaggacg
     6481 attgaaattt gaaatatggc tttccattat gaagaagcga atgcgctgta cgaaaaaatc
     6541 caaaccagat tatagcaatc ttcctgttgg attgtttcaa acgacacaat ttttattgga
     6601 cctccataca tttttgaccc gttacttctt tcaatatgcc tttccttgga atcaaaatga
     6661 aacgttggtt ttttatcttt tcatgatcag taactttaca ttagatacac ataactcgca
     6721 tgtccaggag tttttgataa gcgaaaaata caaagacgac gtagtagctc aattgaatcg
     6781 aacgtttaaa caatcgatag aagaatattt ctctttttat gtttttccag aagagatttc
     6841 gcaaaagatt gcgttgacag tgatgcaaaa tcacgctcat tttgcctatt ttagaggatg
     6901 gatgaatatt tttggccaag aaggattaaa acagcggtta actgaagtag aagataaaca
     6961 attcctggtt ttatgtgaaa gattggtcag acaaacattt gctattgttg attttactga
     7021 agtagacgag gaaaatgccc gctttgaatt atttgctcgc tatgcatcta ttttacggac
     7081 gctgatcaag cagttagaat tacgtctgca aattgcttat gatcttcctt atgaaaggct
     7141 aatggcggat atcgtgaaag aaatcatcca ggaacaattg ctgaatacga tcaaatatga
     7201 tttagtcgaa tacgagaaag aaaagcagta tgatatcatt ttgactagcc aattgaaaga
     7261 gtacccgtca cagaagtccg ctaaagtttt tgtattcact agcaacgaat ataattatga
     7321 tttcccctat ttgaaccaat ttttaaaaga atgttattta gaaaaattga atttaaggat
     7381 gaataaaaca tgaaaatcgt gatcattggt gcttcccatg aaggcatcac agctgcccta
     7441 aatctaagaa aacttcaacc gaaagcagaa gttttactga tcgatgaaga tcataaagat
     7501 ggactaggct atgtttcaaa tggaatcaac ctttatttaa aaggaaagat acgtagttta
     7561 gctgaagttg cccataacat gcggactctt caagaaagtg gggcaaaact gatcaccgaa
     7621 tggagagtaa ctgaacttga tccagacaaa catcagctca tcctcgcatc caaagaagga
     7681 aaaaacgaga ccataaccta tgataaatta atcgtagcga caggttcctc tcctgtcacc
     7741 ttgtataaac aaattgaagc tgaaaatgtc tacacctata aaaatctagt gcaatctaag
     7801 caagtacttg ccgctttaaa agaagcaaaa gaagtagtga tcttcggtgc tggttacatt
     7861 ggattggaat tagcagatgc tttgagaaat aaaggataca ttattcatct agttgattat
     7921 atgccgaatg ttttatcaag atatttcgat aaggatatga tcaacagttt tcaaaatcag
     7981 ctgcaaacta agcaaataaa tttttatcca aatgaattct tgatcgattg gaaaaaatca
     8041 gaagagaaag tagtatccgt acaacttctg tctcaaacaa taaaggcaga tatggtcatt
     8101 ttttctgccc aaactcgtcc taatacgaca ttgttaaaag aaaaagtggc tttgtatgag
     8161 gatgagactg tgatagtcaa tgaatatctg cagacaagtg atccggacat ctatgcgatt
     8221 ggagatatcg tcccagtcag ttttgacaaa aataaacggc atctcttttt acctttagtt
     8281 actcgagctg tacatatggc aagagcagta gcgctaactt tgagtggtca gccaaccgct
     8341 tacgacctca gacaaaagat aacagcagca gtcattatag actacttttt aggaactgta
     8401 gggctgacag aagatgaagc gccattcttg gaacaaaata ccggttcgtg ttcaggagaa
     8461 tttgatctat ttccacaata cgacgaagaa aacaaaacag taaatgcaaa gctgatctac
     8521 catccggata cattagaaat aattggcgga cagctgataa gccaagaatt cttattatca
     8581 gatttgaacc tgcttgcgga tatcgtgaaa cacaaaacta cgattccaca gctagcagtt
     8641 gaggattttg gatttctgtc tgaatacacc tctcgttttc actacctcaa tgagttagct
     8701 tttaaagtac tagctaagag agcaaatagc aatagagtag ataaaagact gtgagaaata
     8761 tcacggtctt ttattgtgtg gggatataat aaatattaat caatcgagaa taaaaaattc
     8821 atataatgaa atagcggaag aattttttga gcataatatt cgccaaaaat ttatcaagtg
     8881 ataaaattgt tgtataaaat gattatttca ggaggaggag ttaaatgttt ttagcattaa
     8941 atgaaattat gcattcaaag ttacgttatg cgttagttgc aggggtgatg tttttgatcg
     9001 cctacctggt attttttttg actgggttag cttatggctt agctcaagat aatcgaacag
     9061 cagtcgacaa gtgggaggca gattcgattg tgctttcgaa agatgcaaat tcaaatctag
     9121 ggatgtccat gattactaag aagatagcag aagaagtcga aggtgggaaa gtggcgtatc
     9181 ttgcccaaac acctggagtc gtgactagta aagacagtac agaagaagga aaaatcaatg
     9241 ttagcttctt cggtatcgat aaaaatcaat tcatcatgcc caatcttgtt gaaggaaaag
     9301 cttttgataa cgatgatgag gcagtaggcg atattagttt gaaagaagaa tatggtctag
     9361 ccataggaga tacagttaag ttatctggta gtgacaagac gtttaaacta actggcttta
     9421 cggatcatgc caaattcaat gtctcaccag ttctctatac aaccatcaat gcttaccaag
     9481 aaatccgctt cgaaaaagaa gatacaagtg aaaatgcacg gattaacgca atagttgtca
     9541 gaggtaaaat cagtaattta cctgaagatt tagaacagat caaaatttct aaattcatca
     9601 atgaattacc tggatataat gcgcaagtat tgacttttgg atttatgatc ggatttttga
     9661 tcgttattgc ggctattgtg atcgggattt tcatttatgt actgacgatg caaaagatta
     9721 atatctttgg tgtaatgaaa gcacaaggga tcacaggagg atttattgcg cgttctgtag
     9781 tggcacaaac attcatttta tcatttgttg gtattcttct tggtctatta ggaacagtgg
     9841 gaacatccct tgttctacct gatgcggttc cattccaaag caattggctg ttttttggcg
     9901 tcattagctt gttgatgtta gtagtagctg tgttaggcgc actattttct gttcggacaa
     9961 tcgtaaaaat cgatccgttg aaggcaattg gataagaagg aggaagaaca tgaaagcaat
    10021 agaatttaaa gaagtagaaa aaagtttcca agatggcgac caagtagtta aagcattgaa
    10081 agaaacaacc atgagtatcg ataaagggga atttgttgca atcatcggtc cttctggttc
    10141 aggtaaaagt acttttttga ctttggcagg cggattgcag acgcctagca gtggctctgt
    10201 atttatcaat gatcaagaat tcagtaatca aaaagaaagc gaacgcgtta aactacgttt
    10261 taaagaaatt ggctttattt tgcaagcttc caatctggtt ccttttctga cagtaaaaaa
    10321 gcagttatta ttagtagata agattaaaca tgaacatcgt caaaaagaag caaacgaatt
    10381 atttgagcag cttggcgtgg ataagttgat caacaagtat ccagaagaac tatctggtgg
    10441 agaacggcaa agggtcgcaa tcgctcgtgc attgtacaat gatccgtcca ttattctagc
    10501 agatgaacca actgctagtt tggattcaga aaaggcacga gaagtagtcg atatcttggc
    10561 aaaagaatca aaagacaaaa ataaagcaac gatcatggtt actcacgata cacggctgat
    10621 tgacaaatgc gatagaatat ttatgatgga agatggtgta ttgaaagaaa aagaataacc
    10681 aaaaagcagg tggaaaaatg gtccatcgat acaacaaaga tacacagcaa aaaattttga
    10741 aagaagcgac tgacttgttt atgtccaagg gatatctcgg cacttcaaca agagaaatcg
    10801 cacaaaaagt tgggatcacc cagcccaatc tctatcatta tttcggtgat aaagaaaaac
    10861 tatatacggc agttttagaa aaccacttaa aagatgttgg aaaagctttg agagaaatcg
    10921 ttcaaacaag cgaaacggga tttcaagtaa ccttgacaaa aatggcgcag tttttgattg
    10981 agacacatct ggtcgatttg tttctgatgc ttcacgatct tgaaagcaat ctttcaaagg
    11041 aaacaagaga tcatcttttc tttctatgga agaaaaatta tcgtgaacct tttgaagaaa
    11101 tcttttcagc aaaccaatct gtgctaagaa atgggatttc aaaagaaatc gcagcaaggc
    11161 attttttctt agtgttagca ccttacatca ctcggtcgaa aagacctgtg gaagaaccgc
    11221 taaatgtggc gcaattgatc gatttatatt tgaatggtgt attaggagaa gcgtaaagct
    11281 gttatatagg gagaaaatag ttaggtgaag aagcaagata tgacgaagat cgttatatct
    11341 tgcttcttta tataggtagt ttttcataaa tacaaatttg ttattctctc atgattatca
    11401 gggtatattt ttatttatta tgtcggtgtt ccaggaagtg ttttaattta ataacgaaga
    11461 aattaattgt ttgtagaatg aaggaaacta tttattgggt agatgaatca ccacgaatag
    11521 tcaaaaggag atttcaggat atgccaaaaa tgaaaaaaat cattttagca gtaatgttat
    11581 tttctacggt tatatctttt acagcccctt ttttgcttac ttaagctgat tttaagtttt
    11641 ggcccttagt ctggatctac gaggtgagag tcaagaatga aaaaaataat tggagctttt
    11701 gtatttgcaa tcattatcat tattatctgt tttcaaatag atgtttttgg tcaaaaaaag
    11761 atagttggac aaacggtcag cagtacgaac agtacggtta caaagacaaa ggaatcattt
    11821 gatgatacag aacaagagac attaatcaaa atcggaaaaa atgaccgtac actatataca
    11881 gatgactcgc taactacccc acttgcaact ataaacggcg gagagctggc ggagtttctt
    11941 tctgaaacaa aaaatgctta tcagatcaag acaaatgatg gctatactgg atatttagcc
    12001 ctcgaagatg gaaccaaagt cacaaagaat atacaaacaa aaccggaaga gctatctgat
    12061 gcagtgattg tattagatcc tgggcatggc ggaaatgata ctggggcatt aagcaatgat
    12121 gaacaaacag aagaaaaaga tataacatta agtacagcca taaaagtaaa aaaggcgcta
    12181 gaggatgctg gagcaacggt ttatttgacc catactacag atgaattagt acagcttgga
    12241 gatatatgcg actacagtga agaaaaaaag gcagatgtct ttatcagttt acacgcagac
    12301 tctacggaat atgcgaatga agcaactgga atcacgacat attattacta tggacaagag
    12361 gaaacattag cacagacaat tgctgactcc ttcactgatc tgcctctgga ttcaagagga
    12421 atctccactg ggaactatca agttcttcga gaaaatcttc aaccttctat tttaatcgag
    12481 atgggttata tgaataatga tagtgattta aaggaattag taacagacag ttatcagcag
    12541 cagattgctg caagtttgac acaagcattg accacttatt ttcagtaata aataagccca
    12601 gccaaacaaa aatagctcct catatttttc gtttggccgg gcttattatc gcagatgcaa
    12661 gcttttaaac accgtttaag gaagaggtcg tgagcctgcc gttcaactct gagtcacaag
    12721 gagcaatttc aaaaaacagc ttctcatgtt tgatgactat tgtgacttgt gaccgaggag
    12781 ttggcatgag aacaccgttt aagaaaaagg ttgtgaaatc aacgttttgt tctgagtatt
    12841 agagaaacaa acggaaaaat agttcctcat attttgccgt ttgttgagct aataccgagg
    12901 atcagatgat tgaacaccgt ttattcggaa caagggactg tgacatgact tttgtcacag
    12961 ttccattatt tttttagtag cttcaaagct ttttctgtat tggcaaaatg aagtttggca
    13021 tatttttcaa gcataggcat gccgcctttc tccaatagtt ttgccgctac ttggtcagat
    13081 ttcgttccag ctcctgacgg aagggtgatt ccagcttcag ctgatgcttt gtctaactct
    13141 tccaaaaagc tggcacgact gatgatagaa gcagcagcaa cagctaaatg atattgttct
    13201 cctttagtaa cgaagtacag tttttcttgt aattgatttt tttctaaacg cgcatatttt
    13261 ttaaaatttg cttccggcgt aaactgatcg atcaaaatcg cttctggttt ttctggctcg
    13321 atttttttta gcagcaaatg gatcgcttga ttgtgcaaag cgactttcat ccgaacggca
    13381 ttgtattttg gttggatctc attgtatttt tctggagata agacaagcag tttatagggg
    13441 atggtttttt ttaagatgtc agcaagctgg atgatttggc tatcttttaa ctcttttgaa
    13501 tctctaacac caagtttacg taattctgca agctgatcac gtttcacata agcagcacat
    13561 actgttagcg gaccgaaata gctgccattt ccgacctcat cactacctaa aacggataat
    13621 tgactgatat tttttggcaa agaagaggct gtttttgctg gcttggcttg tttatcctgt
    13681 gcctcccccc atttatcagc ttctattgct gcattcgtac cttgaaacat cacttttccg
    13741 gatgtataag cggtaatggt cgtcccgtct ttttttgcag caaagctagt ataaggaatc
    13801 tgtttattta acaaataaga gcgatagtat gctttcattt gactaagttg gtcgtttgtt
    13861 gctttgataa tgacgttgct cataaaattc tcctcgtttt tatacataaa actataccgc
    13921 aactcacgac ttttcgtttt ctaagagatt gcgagacata gcattttttt aaagtttatc
    13981 atataatagt taagtatgtg aaaaatattt gtggagaacg gtgaaagaac caacttcatg
    14041 gtacactaaa taaaactttc atcaaatagg aggaaatgat tcgtatggcg cacgaaaaaa
    14101 cacgctacaa agcggtgatt gccaaccaaa catatactat tatcggacgg gaaaccaagc
    14161 atcatatgga tatcgtaacg aagctgatca atgaacagtt agcagaattg aaacaattat
    14221 caccacaaat ggataatgaa caagcagcaa tcttgatggc tgtcaatgcg ctttcggatc
    14281 aattgaagaa acaagaaaga atattggagc tggaagaaga aacagcagaa ttaaagaaaa
    14341 aaatgatcaa atttacagag cttgaaaatc gtgtgaaacg cattgaagca atcgagaacg
    14401 aagctcggga agtattgaaa gaaaatggtc aagcagatca taccattaaa aatcatgtgg
    14461 aagcacaaca gattttaaac gagaaacgaa aaggacaaat taaacaaaaa tcttctaatt
    14521 aagatccttt tatcaaaata agaaaaattt aaaaaagtta gaagagaaag gaacgtagca
    14581 tgctgagttt gctgatttta tttttattgc taattgcgtt tttttctgga gcacgtcgcg
    14641 ggtttgcttt gcaaatcata ttcgcgattg gatatgtatt gtcatttatt gctgcccagc
    14701 atttttacaa accattagca agtcatttgg aattatacat tccataccca gctgtcaccc
    14761 caacatcaaa gttagcattt tttgatcagg tatttgcctt tcacctagat gaagcttttt
    14821 atgcaggaac tgcttttctg attattttgc taattggctg gctgcttacc cgatttgtgg
    14881 gggtttttgt ccatggattg acgtatgtgc cgatcttgag acaggtagac tggattgcag
    14941 gaggcatcct gtcactaatc atggcttacg ttactatatt tttgattttg cagttacttg
    15001 cctttgttcc attagatgtg gtccaaaatc aattcggcga gcatagtttc gctcgtttta
    15061 tcgttgagag aacgccattt cttaccaaca agatgtatga gctttgggta acgaatattt
    15121 tgaattaagg aaaatagtgc tggaaaatat ttcgtttttt gtacagagta gtttatttaa
    15181 gaaggctgcg tagcgtatgt ccggcacagc tcttttttca tagtgaaaga taaggtgata
    15241 aaatgaataa ccgaatcctt gaaacattgg aatttgaaaa agtcaaacag atggtcagac
    15301 aatttgtagt gactgcacaa ggaaaagaag aactggcaga attggtacca gtgagtgaga
    15361 aacaaacgat cacaaactgg cttcaagaaa ctgaggatgg attgaaagtg caacgtctgc
    15421 gaggcgggat ccctatacct aaaattgaaa atatccgtcc ccatatgaaa cggattgaga
    15481 tcggagcaga cttgaatgga gtggagcttg cacaagtttc cagagtattg tctacgacaa
    15541 gcgaattgaa tcgtttcatt gatgatcttt ctgacagtga aatcgaattt gcacgtttgt
    15601 atatgtgggc agatcagtta gtcacgatcc ctgttttgag ccgccgttta aaggaagcaa
    15661 tcgatgaaga cggacgcgtg actgatgacg cttctcctga gttaaagagc ataagacaga
    15721 acattcggcg aagtgaacaa gctgttcgtg aacagttaga tgggatcgtc cgtgggaaaa
    15781 atgcaaagta tttaagtgat gcgatcatca ccatgcgaaa tgaccgctac gttatcccag
    15841 tcaaacaaga ataccgcggt gtatttggcg gtgtagtcca cgatcaaagt gcttcaggtc
    15901 agactttatt tattgaacca aaacaagtgg tggatttgaa taatcgtttg cgtcaatacc
    15961 aaatcgctga acgaaatgag attcaacgta ttttaagtga actttctgct gaacttgttc
    16021 cccatcgtca agaaatcatc cacaatactt atgtgattgg taaaatggat ctgatgaatg
    16081 caaaagcacg atttggtaaa gaagtaaaag ccatcgtgcc gggaatcagt gaagacaatc
    16141 atgtcgtctt gaaacaagca agacatccac tgatcgatca ggaaaaagta gtttctaatg
    16201 acatcacaat cggaaaagac tatcaagcca tcgtcattac aggacctaac acaggtggga
    16261 aaacgatcac gttgaagact ttaggtctgt tacaactgat ggggcaagca ggattaccga
    16321 ttcctgcagg tgaagaaagt caaatcggta tttttgagga agtatttgcg gatataggag
    16381 acgaacagtc gattgagcaa agtctatcga ccttttcttc tcatatgacg aacactgtag
    16441 atattttatc aaaagtcaat gaaaaaagtc tggtattatt tgacgagctc ggtgcaggaa
    16501 cagatccgca agaaggtgct gctctagcaa tcgcaatctt agatgatctt ggtaaaaagt
    16561 ctgcttatgt gatggcaaca acgcattatc ctgaattgaa agtttatgga tataatcggg
    16621 caaatacgat taatgccagc atggagtttg atgtggatac attaagtcca acgtatcgtt
    16681 tgctgattgg cgttcctgga cgaagcaatg cgtttgaaat ctctagccga ttaggtcttg
    16741 atacagaagt tattgatgaa gcgaagcagt tgatgaatga tgagagccaa gatttaaacg
    16801 aaatgatcac agatttagag aatcgccgga aaatggcaga gaccgaatat ctggaaatgc
    16861 gtcattttgt ttctgaggca caagaacttc acgatgattt gaaagaagct tacagctatt
    16921 tctttgaaga acgtgaaaaa gaaatggaaa aagcaaagaa aaaagcaaat gaagtcgtgt
    16981 cagaggcgga agaaaaagca gaaaaaatca ttgctgatat tcgtaaaatg caacagcaga
    17041 ttgggcaagg aaatgtgaaa gagcatcagt tgatcgatgc aaagacccaa ttagcaaatc
    17101 tacatcaaga agaaactttg aagaaaaata aagtattgaa gaaggcgaaa gaacaaaaaa
    17161 cgttgaaacc gggagacgaa gtgttggtta cgacttacgg ccaaagaggg acgcttttac
    17221 gtaaaaacgg gaatcagtgg caagtagaaa tcggtatatt aaaaatgaat gtgtccgaag
    17281 acgagctgac gcctgttgct cctcaaaaag agccgacaca acgagttatc catgctgtac
    17341 gatctgaatc tagtagtcac gtgccgaatc aattggacct tcgaggaaaa cgatacgaag
    17401 aagcgctaag tgaggtagat caatatttgg attctgccat tctagctggt tatcctcaag
    17461 tgactattgt ccacggaaaa ggaacaggcg cacttcgcaa gggaattact gattatctta
    17521 aaaaccatcg gagcgtcaaa agctttgagt ttgcaccggc aaaccaaggc ggaaatggtg
    17581 cgacaattgt taaatttaag taaagcttac taaaggtaag cagatggttc gctatccaag
    17641 tatagaaatg ctatactagt catagatttt aaaaatggag gtctttgata tgtcacaagt
    17701 aattacagat aagacattta atgaagaaac agataaagga cttgtattga ttgatttttg
    17761 ggcaacttgg tgtggtccat gtcgtatgca agcaccgatt ttggatcagt tggaacaaga
    17821 atatgatgaa gaagaattca gaattgctaa aatggatgtg gatgaaaatc cagaaactcc
    17881 tcaacaattc ggtatcatga gcattccaac attaatgttg aaaaaagatg gtcaagtagt
    17941 agaaaaagct gttggtgttc attcaaaaga acaactacgt caaatgatcg atcaatattt
    18001 ataaaaactt acagcaaaaa taagctgagg gtgggactga atccagttcc gtcctcggtt
    18061 tttttgcggt tgagttaagg ttattcaaat taatattttt tgagtataat tacgttgtca
    18121 ggctggattg tttttttgac agacatgcta taataacttt aattgaatat gaaggggagt
    18181 accagatgaa cgaacgaatc aaaaacaaat tagctttact tcctgatcag cccggctgtt
    18241 acttgatgaa agataaaaat ggaacgatca tttatgtcgg caaagccaaa atattaaaaa
    18301 atcgtgtccg ctcatatttt actggtagcc ataatacgaa aacggaacgg cttgttagtg
    18361 aaatcgaaga ctttgaatac atcgttacag aatcgaatat cgaagcattg ctcttagaga
    18421 tcaatctgat aaagaaaaat gatcctaaat acaacattat gttaaaggat gataaaacct
    18481 atcctttcct taagataacg aatgaaaaat atccccgttt ggtgattact cgtaaggtac
    18541 taaaagacaa agcactgtat tttggaccat atcccgatgt cggtgcggcg aatgagacaa
    18601 agaagatcct cgatcgatta tttcctctga gaaaatgcaa acctagtcag acgaaggagc
    18661 cttgtcttta ctatcatttg ggacaatgcc tgtgtcctta ttatttcgac gtagatccga
    18721 aagtctatac gaaaatcgtt gatgaagtga aaaacttttt aaacggagga cacgaaaaaa
    18781 tcgagtcaga gatccatcag aaaatggaaa aagctgctga aaacatggag tttgaaaaag
    18841 ctgcagaata ccgagaccag atccgtgcca ttgagacaat catgactcgc caaaaaatga
    18901 caaatactga tttactggat cgagatgtat ttggttatgc gattgataaa ggatggatgt
    18961 gtgtccaagt gtttttcgta agacaaggaa agctaattga acgagatgtt tcaatgtttc
    19021 ctttctataa cgaagcagaa gaagatttct tgacttacct cggtcagttt tatcaggaaa
    19081 atgagcattt cattcctaaa gaagtcttga ttcctgatac gatcgacaag cccagtgtag
    19141 aagctttgtt agctacgaaa gtgctgcaac caaaacgagg agaaaagaaa aaattagttg
    19201 aattggcgag taagaatgcc tcagttgctt tgaatgaacg gtttgatctg attgccagaa
    19261 aacaggaacg gacgatcgga gcagttgaac gtttaggaaa cgcgatgaat atcccgaccc
    19321 caatacgtat cgagtctttt gataactcta atatcatggg gacgaatcct gtggctgcga
    19381 tggtcgtatt cattgatgga aaaccggcaa gaaaagaata tcggaaatat aaaataaaaa
    19441 cagtcaacgg accggatgat tatgcatcaa tgagagaagt catctatcgc agatattcgc
    19501 gagtgattcg cgaagggttg cctttacctg atttgatctt gatcgatgga ggaaaaggac
    19561 aagtagatgt ggcaaaagat gtcttagaaa atcaattagg aatcgatatt cctattgctg
    19621 gcatggtaaa aaacgataaa cataaaacaa atgagttgct attcggtaca gatttggctg
    19681 tagtaccgct tgagcgaaat tcaccagagt ttttcttact ccagcgaatc caagatgaag
    19741 tccatcgatt cgcaatcact ttccatcggc agctacgcag taaaaacagt tttgcttccc
    19801 gattagatga aattgaaggg ttaggaccaa aacgaaaaaa aatgctgctt aaagagttca
    19861 aatcgttgaa aaatatcaca gcagccacga cagaagaatt acaagaaatc ggactgccga
    19921 aaaatgtcgc acaaaacgta tttgataaat tacatcagga atgaggaaag ggcactttac
    19981 aaagaaagta aagtgtccta tcattttatg tacaacaaaa agagcgttta cgttttgttg
    20041 gtaaataata aatcgcttac gtctggtgtc taaggatata tgtgatttct tgaagaagga
    20101 gtgaagaaat gttagaagtc aaggatcttg ttaaaacttt tgggaactac acagctgtcg
    20161 atcatgtctc tttccaaatc cccgatggaa aaatcatggg gctaattggc caaaacgggg
    20221 ctgggaagac aacgactttt cgtttgatct tggatttttt gacacaagac caaggagaag
    20281 tgttatggaa tggccatcat cttggggaga aagattatga tgtcatcggt tatcttccgg
    20341 aggaacgggg actttatccg aaagtaacga ttcaagacca actgctttat tttgcagaac
    20401 ttagagggaa atcaagaaaa gagattgaac caaaaatcga cttttggatg gaaaaatttc
    20461 aggtaaaagg gaaaaagacg gataaagtaa aatctttgtc taaaggaaat cagcaaaaag
    20521 tccagctgat cgccacgtta atccatgaac ctaaattgat cattttggat gagccattca
    20581 gcgggttaga tccggtaaat gctgaattgc taaaagatgg aatattggaa ttgaaaaaac
    20641 atggttcatg tgtcattttt tctagccaca atatggataa tgtagaaaaa atctgcgatc
    20701 atttgatcat gttgcgaaac ggaaaaatgg ttttgaatgg aaaagtacat gagattcgtg
    20761 aatcatttgg ccgtacgaag ttattcttgg aatctggttt aagccaacag gaagtagaag
    20821 aaatcgaagg cgtgaaaaaa gtgatccttc atgaagacaa tacgttagag attaccttag
    20881 aagatcctga agctgggaaa gaaatcttta ctcgtgcgac tcagttcggc tacattccta
    20941 tgtttaacca gcaaccgcca actttggaag agattttcaa attgaaagca ggtgaaatcg
    21001 atgcgtaagt tttggattat taccaaagat gtctatttaa aaaatgttaa gtcgatttct
    21061 ttcttgatca tgatattagt cccttttatc atgctgggta tcatttatgt agcaggaaac
    21121 tttgctcaac aaaatagtga tgtcaataaa atcggcgtat tggctgataa tgagcaaatc
    21181 agtcagcaaa tgagccaagt caaaacagat gattttgagt tcaaagcagt tgattctgaa
    21241 gaaaaagcac aaaaagagct ttcggaagaa aaaatcgatg cgtatttagt agtctcaact
    21301 gagaatgata agataaatgg gatgctttat agcgaaaatt cattaggaca aagtacacaa
    21361 ctactgatac aacaacaact gactggcttg caatcgatgc ttcgagcgag tcaattaggc
    21421 atttcaccag aagaagtagc tagcttgagc caacaagcaa gtttgactcg acaaaaagtc
    21481 aattttgatt caaaaggaaa aatgacagtc ggagaagata actcgacagt ccaatacgtc
    21541 gtaagttatg tagcaacgat tgtcttgttc attattattt tgacgtatgc gcagatcatt
    21601 gcacaagaga tcgcttctga aaaaggcacg cggatcatgg aagtgatcct gtccagtacg
    21661 actgcacaaa agcattttta cggaaaatta acaggtgttt tacttgttgc attaacgcaa
    21721 atggcattat acggtattat ttttggtata ggatacagtc aattcaaaga tatggaaatg
    21781 gtcaaaaata tattagacgg tatctcattg gataacttga ttggaccatt cttgtggtat
    21841 tctcttctat tcatgatctt tggcattttg attttttctg ttttagcagc tttatgtggc
    21901 tctttggtca acaaagcaga agatacagca aaagcaattt tgccagtgac ttatctatct
    21961 ctaggcggat acatgcttgg attgatacta ggagcttcag atccaaacaa tatcgttatt
    22021 cggatcactt cgtatattcc attcttatct tcatatatta tgccgattcg cttagcaaat
    22081 gagacagtcg gtatgggtgg agcattgatt tccttgttgg tacttgtagc cgtaacattc
    22141 ttattgatga tcttgtctgc aaacatgtac aaatcgaatg tactcgttta tagcgaaggc
    22201 ggattatgga gttcattgaa acaatcgatt tcaattatga ggaatgaacg aaaaaaagca
    22261 taaaacaatc ctaaaataca gaaggaagct ggacatctat tgttcccagc ttctttttgt
    22321 atagacctat tttatttatt ttactatatt ggatatatat tcattgaaaa tagtgtataa
    22381 caactttata atattgagta taccaatttt tggaggcgtt ttaatggacc gttatttaga
    22441 tttactttca gaaaaattcc cctcaacgga ggatgtcatc acggaaatca tcaatctcga
    22501 agcaattttg cagcttccaa aaggaacaga gttatttttg agtgatatac atggcgaatt
    22561 tcctgctttt gatcatattc tgcgtatcgg ttcaggaaat cttaaagaaa agatcaaaga
    22621 ccttttttct gaacaaatga cggaaacgga tatcagccaa ttcactattt ttacggctta
    22681 tccagaatat gcattaacga ctgattggta caaagaacaa gataaatcgc agctgatcca
    22741 tcatttgatc gatcttttac ggtttacgac agtcaaatat acccggtcaa aagttcgaaa
    22801 aggattgccg aaagaatata gctatatcat tgaggaactc ttgtatatcg atgaccgtat
    22861 caatggaaaa aaagactatg taaaaaaaat cattgaacaa cttgttttga tgaaggaaga
    22921 agaaaaattt ttaacgaaac ttgcacagac gatccaaaaa tctgtcatcg atcatttgca
    22981 tatcgttgga gatatttttg accgaggaac tcaagcagcc aaagtgatgg atcgaatcat
    23041 ggatttttct tctgtagata tccaatgggg aaatcatgat attttgtgga ttggtgcgta
    23101 ttgcggctct gaggcatgtt tactgacact tttacggatt gcagcacgct ataattattt
    23161 atttgaatta gagaaagaat atgggttgaa tttacgtcca ttgttttctt ttgctcatca
    23221 aacttatcag aagaatccag tatttgcgcc taaaaacagt aaaaatctat ccgaaagaga
    23281 gcaagaagaa ttagaaaaaa tccatcaggc actttctatc cttcaattca aatcagaaca
    23341 tcagttattg gatcgtcgac ctgaatttaa aatggacgat cgtaaattgc tggaaaaaat
    23401 cgactatgaa gcatcaacga ttgatttaga aggtcagctc tatgagttga aaggaacttg
    23461 ttttcaaacg atccagccag atgatccaaa aaaattcttg gaagaggaac aaaaagtgat
    23521 tgatagtttg atgtattcat tccagcactc tttgagaatg caaaaacata tgacgttttt
    23581 gatcgaaaaa gggggaatgt atctcacgaa caatcagcat gtgctgtttc atggctgtat
    23641 acctgtagac gagaagggac agctcttagc atttgaatta gaccaatctt atcgcggaaa
    23701 agaactgctg ggcttttttg aaaaacagat aactgaaagc gcaaaagatc tgactgcaaa
    23761 agaagattta gcaaccgatt tgatttggta tgcgtggagt gggccatttt cgcctttatt
    23821 cggcaagagt aaaatggcga cttttgaacg ttattttctc acagagcctc atacgcacgt
    23881 cgagaaaagc aatccttatt atcacttgcg agatgaagag tggttcagtg aaaaaatttt
    23941 agcagaattc gacacaaaag aagaagggtc agcaatcatt aatgggcaca cgccagtaaa
    24001 agtaaaaaaa ggggagtcac cgatcaaagc aaatggaaaa gtatttgtca ttgacggagg
    24061 tttatccaaa gcttatcaaa aaacgacagg aattgccggt tattcactat tatgcaattc
    24121 ttatggtttc caaatcgtga cacattatcc gtttttgcct attgaacaac aatttgcaaa
    24181 aaaatccgac caaacaaatg tgaaaaaagt aatcgaacag caactaccac gcaaactcaa
    24241 tcgtgataca acaaaaggta tggaactgca gcagcagatt acccagctca aaaggctgtt
    24301 ggactatcga aaggacgatt gagaagaatt ccagtgaaca ttgttccata gactggagac
    24361 tgagacaaaa gtcgtgtcgc agtctctttt tccggataaa cggtgttctt ctgccaactc
    24421 ctcggtcaca agtcacacta ttcaacaaac atgaaaagct gttttttgaa attgctcctt
    24481 gtgactcaga gttgaacggc aggctcacga cctcttggta taaacggtgt tcaaaagcca
    24541 gtttctcggt attaagccaa atttatgcaa aaatgtgaaa agccattttc acaaaatttt
    24601 tcttaatact cgaaactaaa cagctttttc acaaccccct cagaaagtct attaaaattc
    24661 attcacaaag aaatcgaata agcgctgttc agatggatgt acacggtggg taagttccgg
    24721 atgccattga acaccgagga ttttatgcat atcatctttg ctttgaaccg cttcaacgag
    24781 tccatcgtta gaaaaagcga tggcttttaa taaaggtgaa agatctttga ctgcctgatg
    24841 atggtaagaa ttcacaaaat atggatcggg gagaaggttg cttaacaaac tgtctgaaac
    24901 aatcttgact tcgtgagtcg taaattgcgg ggcagtgggc tgttgttcat gttttactga
    24961 ccactttgga tataatgaaa gatcttgata aagtgtacct tccaaaacga cattcaatag
    25021 ttggagtccc cggcagactg caaaaattgg ttttctttgt ttcaatgctt ccaagatcag
    25081 tgcctgttca aattggtctc gttgaatatt tgtttccgtt agctttggat gaggatcttc
    25141 catgtaaagt tgtggactga catcttgacc acctgcaaga ataagtttgt cgatttgtcc
    25201 aatcagttga ggagctgaat ctggagacat gactggcaga ataagaggca atccttgagc
    25261 atctaaaact gcatccacga atccttgcgg tgtatagctc acttgatttc cttggaaagt
    25321 atctgttgct cgaatcagtt gatttccggc aatgccgatg attggtttca tttgtattcc
    25381 ccctaaagta gacttattgt aacaaggatt taaaaaaaat taaactatgt aagacttgcc
    25441 cctatactta tctgttcttg ctgtgttact atctgtaaag agaaatttcg tgtgaaattc
    25501 atgacgatag ttgaaaatat tgcttaattt gttataataa aaagtaagca gaaataaaaa
    25561 ggttgggcgt aagcttcaag gatctagttc ttcaaggggt gagaaggaca ttttattgaa
    25621 ggggcctgcc tatactaagt tatctttaga ggtacatact ttcaatgaaa aaagaaagca
    25681 tgtacctctt ttttaaatcg aaaggaagtg aagcaagtgt tagaagcttt ggaacaaatc
    25741 cgaaaagcag aagaagaaaa tgaaaagcgt gctcaaacac tgcggcagca gctgaagatc
    25801 tatgcaacag aaaaagagca ggaattggaa acgatccaaa caaactatcg acaacaacta
    25861 aaacagttgg tggaagaaaa agaacaattt gaaatacaag aattagaaaa agaaaaaatg
    25921 aaattgaaag cagaagcaga caaggtgcaa gcaactttga aagatcaata tcaaaagcat
    25981 cagagtcaag cgatcggtgc gatcattgaa agggtgaaag aaacttatgg ccgtcactaa
    26041 gatggaaaaa gtgaccctca tttcagataa aagaaatcag gagaggattt tacaagcagt
    26101 acaaggaatc caaaacgttg aaatccggga tctgtatcaa aatagaacaa acaaccagtg
    26161 ggtggagaag tattttccag ataccacgat tattgataaa gaagcaaaat taagcgcttt
    26221 aggtaatcgg ttgaaagaca tccaagaagc tatccaattc atacaacacc acggcgatag
    26281 ccgtcaaaag aaactccatt tgaaacgtca agaactttcc ttacaagatt tggaagccaa
    26341 ttattccgaa gaaaatttca taaataaatt gaaagaagtc cttgatttaa aagaaaagtg
    26401 ggaacaacta actgaagaac gtgatcgttt agaagaagag gaagaatggc ttcttcattg
    26461 gcaacagctg gatattgctc caagtatgta tgcaagtaag acgaccattt ttgagatggg
    26521 gactgtgagt gcagcaaatt ttgaaagttt caaggaagaa ctgagtaagc ttgatgacgt
    26581 ttatatggaa gaaatagatt atacgccaaa acaagtttat ctttcgtatg ttgctttaaa
    26641 aaaatcagtt gctgttatag aagaaattgc tgggaaatac gggttcaata aagaaacgta
    26701 tccttatgaa caaaatccta aagaaaaact gcaagagacc aagcagcaat tatccattgt
    26761 tcacgagcag caaaagaaga taacgacagc gttaggtcag tgcagtggct atatccaaga
    26821 atttgaatgg gtagaagaag tcacacttgc cgttgcagaa cgagaaaaaa taaaagaacg
    26881 gtttatccat gcgacttatc tgatcgtttt gcaaggttgg gtagatgcag aagaaaaaca
    26941 gaatttgctg catgtgttaa acgaaaccgt atcagaagaa gatatatacg tcagtttcga
    27001 aacaccaaac tcagaagaga ttcagcagga agtccctact aaattgaaaa accatccgct
    27061 tgtcgaacct tttgaattgc tgactgaaat gtacagtctg ccaaaatatg aagaagtaga
    27121 tccaacacct tggatgacac cattttatct tgtcttcttc gggatgatgg tcgcagatgt
    27181 tggttatggt ttgctgatgc tcattgggac aatactagct caaaaactac ttgttctacc
    27241 acgcgggatg aaacgtttcg taaaattctt tgagattctc tcgataccat cgatcatctg
    27301 gggattgatc tacagttcat ttttcggaca gtcattacca aaagaactgt ttggcatccg
    27361 cttgcctttc ccaatcctat ctacgacgga tgacgtcaat acgattttga ttttatccgt
    27421 gattttcggt ctgatacaga ttttggttgg attatttgtg gcagcaaagg aaaatatcag
    27481 gagaaaagat tacttgagtg ctgtcagtga tggatttgcc tggcaaggga tcctaattgg
    27541 gatcgttatt gcgttggttg gtgccatgtt gataaaaaat acagcatttg tttaccttgg
    27601 cgctgggcta tcgatcattg ctgccttatg tatcttgatc gttccgatta tccaaacgcc
    27661 gtctaaagtc aaaggtgctg ccaaaggtgc gtataatctt tacggactga ccggctatat
    27721 cggtgattta gtcagttata cacgtttgat ggcattgggt atttcaggcg gaagtattgg
    27781 agcggcattc aatatgctag ttgctttcat gccgccagct gctcgattca gtgtagggat
    27841 ccttttgatc atcgctttac acgcactgaa tatgtttttg acactgctaa gtgcctatgt
    27901 ccatggcgca cgattgcagt atgtcgaatt ttttgggaag ttttatacag gaggcggacg
    27961 tgcctttgat ccgctgaaaa cggcggaaaa atacgtcaac atcaatcaca agaaaaaaaa
    28021 caataaataa ttggaggatt tttatataat gatggattac ttaattagtc aaaatggtgg
    28081 aatggtattt gcggtattag cgatggcaac agcgacgatt ttttcaggga tcggttcagc
    28141 taaaggtgtt ggaatgacag gtgaggcagc agcagcattg acaacaagtc aacccgaaaa
    28201 attcgggcag gctctgattc tgcaattgct tccaggcacg caaggtttgt atggattcgt
    28261 tattgccttt ttgattttta tcaacttagg aaatgacatg tcagttgtac aaggactgaa
    28321 ctttctaggt gcttctctgc cgattgcttt tacaggcttg ttttcaggta ttgcccaagg
    28381 aaaagtcgca gcggcaggta ttcaaatttt agcgaaaaaa cctgaacatg ctacaaaagg
    28441 aatcattttt gctgcgatgg ttgaaacata cgcgatttta ggtttcgtta tttccttcct
    28501 attagtgttg aatgcctaag cataaaggag gaaaagcaga tgaacgccat tgaaaaaatc
    28561 atctctcaga tgaacgaagc agcagagcag gaaagagcgg cgttagaaca agaagagcga
    28621 atgaagatcg atcaaaactt tgaacaaaaa cgtacgcaag ttgaaacaga acatcaaaaa
    28681 caaaaagaaa aacaaatcga attactcgaa aaaaaatacc gtcagctaag gaatcgccag
    28741 caagtagaag tacgacaaga aaatttgaat gcgaagcagg aatttttaag acgattgttt
    28801 gcagatgcgg taactgaaat ggaaaattgg gatgaatctg aacaaatcca gtttatcaaa
    28861 aatgcattgt attcattacc tttgacagga aaagtagcat tcattgctgg agaaaaatca
    28921 gcagcctatc tttctcagac gttgttggat gaatggaaca atgaacttcc ctttatgatg
    28981 gttttaagtg atgaaactgt tgctgatcaa gcgggttttc tgataaacga tcaaggagtt
    29041 cagtataatt ttcttttcag cagccttgta caagatatcc aaggaacgat gagttttgag
    29101 attgccaatc aattattcga ataaggaggg atcagtgtat ggaataccac gaactaaatc
    29161 ccttgattcg cggaagagag cttgaacttt tgtccaatga gacatttgaa cggatgattc
    29221 aaacaaattc tgtcgaagca cttggtgaaa tattgaaatc cacgatttac agtccatata
    29281 tttatgacgg atatgaatat gacttcgaac agaatctttt aaaggagcag agccgtctat
    29341 ttcgctggtt aaaggaatca gcaccggagc ctgaaatcgt ttggatctat acgatgcgtt
    29401 atactttcca taatctgaag gttttgacaa aagctgagat gacaaatcaa aatctggacc
    29461 atttgtatat cgacgatgga ttttattcat tagaaacctt gaaggatgcc attcatacgc
    29521 aagtttcaaa tgaacttcct gaaagcatta tggacagtat acgagaagta cgtgattatt
    29581 ttgaagagtc taccattctt cagggaattg acgtgattta tgatcgacag tttttgacag
    29641 agcagcgacg gcttgccgat aagctaggat atcaagaatt gctgacagaa gtcattgctt
    29701 ttattgactt gaccaatatc acaacgatgg ctcgaggaat attgcagaaa agaacatatg
    29761 ggttcatgac gacggtactt tccagctcgg gaagtatcga aaaagaagta tttttgaact
    29821 ttgtagataa agatatggaa gattacacga attttctatt agagacagat tatgctgatt
    29881 ttctgcgccc tgtcatccgt gagggaaata ttgatctcgt aaagcttgaa cagttgaaag
    29941 atgattatct aagttcattg tatcaagaag cacaaaccca ggctttcgga ccactgccac
    30001 tactagcctt tttgaacgca aaagaggtag aaagcaaaaa ccttcgttta ctggttatcg
    30061 gcaaaagaag ccatttcgaa tcagaaacaa tcagaaagcg ggtgagacaa gtctatgacg
    30121 cataagatcg gtgtagttgg cgacaaagat tctgttttgc cattcaaatt gtttggcttt
    30181 gatgttcgtt atggtacaac caagcaaacc gttcgtcagg caattgaaga aatggcaaag
    30241 aatgagtatg gcgtgattta tgtcacagag caatgtgcag cattgagccc agaaacgatc
    30301 gaacgttaca aggaacaaat gatacctgct atcgtattga ttcccaatca tcaaggatca
    30361 ttagggatag ggctggaaga aattcaaaaa aacgtagaaa aagcagtagg tcaaaatatc
    30421 ttatagtatg aaaaggagat agaaattttg caaattggaa aaatcataaa agtctccgga
    30481 cctcttgtaa tggctgagaa catgtctgat gccagtatcc aagacatgtg tctagtggga
    30541 gatctaggag ttatcggaga aattatcgag atgcgtggcg atgtggcgtc tattcaagta
    30601 tatgaagaga cttctggaat tggtccaggc gaaccggttc gctctactgg tgaagcttta
    30661 tctgttgaat taggaccagg gatcatttca caaatgtttg acggaatcca acggccatta
    30721 gataccttta tggaaatcac acaaagtaat ttcttagggc gcggagttca attgcccgca
    30781 ttggaccatg agaaaaaatg gtggttcgaa ccaacagtag aagccggaga aacggtgagt
    30841 gctggagatg ttatcgggat cgtagaagaa acaaaagtta taaaacataa aatcatggtg
    30901 ccaaatggga taaaaggaac gatcaaaaca atcgaaaaag gttcattcac tattgatgaa
    30961 acagtatgtg tcattgaaac cgaaaatggt gacaaaaatt tgacgatgct tcaaaaatgg
    31021 ccagtaagac gtagtcggcc aatcaaagaa aaattaaacc cggatgcacc catgatcact
    31081 ggacagcgag tgattgatac attcttccca gtgacaaaag gaggagcagc agctgtacct
    31141 ggtccttttg gtgcagggaa aacagtcgtt cagcatcaaa ttgcaaaatg ggccgatgtg
    31201 gacatggttg tctatgtggg atgtggcgag cgtggaaatg agatgacgga tgttttaaat
    31261 gaattccctg aattgattga ccctaataca ggagaatcgt tgatggaacg tacggtattg
    31321 attgctaata catcaaacat gccagtagca gctcgagaag cttctatcta tacgggaatc
    31381 acaattgcgg aatatttccg tgacatgggt tatgacgttg ctatcatggc agactccact
    31441 tcacgatggg cagaagcact tcgtgagatg agcggacgtt tggaagaaat gcctggtgat
    31501 gaaggttatc cagcttatct tggttcacgg cttgcggaat actatgaacg atctggaaga
    31561 gtcattgcat taggatcaga aggtcgcgaa ggaagtataa ccgcaatcag tgctgtatcc
    31621 ccgtctggag gggatatttc ggaaccagtc acacaaaata cattgcgggt cgtaaaagta
    31681 ttctggggtc ttgattccag tttagcccaa aaaagacatt ttccttcgat cgattggata
    31741 caaagctatt ctctttattc aacagaagta gggaagtata tggatcatat ccttcaagct
    31801 gactgggcat ccatggtcac cgagggcatg cgtattttgc aggaagaaca gcagctgaat
    31861 gaaatcgtgc gtttggtagg gattgattcc ctttcagata atgaccgtct gacattagaa
    31921 gtggcaaaat ccattcgtga agattatttg cagcaaaatg cttttgatga tgtcgataca
    31981 ttcacttcaa gagagaaaca attcaaaatg ttgaagatga tcttgacctt tggtgaagaa
    32041 ggacgccgtg cgttgtcgtt aggtgcttat ttaagtgaga tcatggacgg taccgtatcg
    32101 attcgtgaaa ggatcggacg aagcaaatat atcccggaaa cagaattaga aaaactagat
    32161 caaattattg gtgaaatcaa gcaaacgatg caaacgatcg tttcagaagg aggcatgagc
    32221 aatgattaaa gaatatcgta cgatcggtga agtcgtcggt ccattgatgg cggttgaaaa
    32281 agttgctggt gtcaaatatg aagaattaat cgaagtccgt atgcaaaatg gcgagattcg
    32341 tcgtggacaa gtattggaag tccaagaaga taaagcaatg gtccaaatat tcgaaggaac
    32401 aagtggtatc aatttacgtg actcttctgt acgattttta ggccatcctt tagaactagg
    32461 tgtatcggaa gacatgatcg gtcgagtatt cgatggtttg ggacgaccga aagataacgg
    32521 accagagatc ctgcctgaaa agcatttgga tattaacgga gaagtaatca atccagtagc
    32581 tcgggattat ccagatgagt ttatccaaac aggtatctct gcaatcgatc atttgaatac
    32641 attagttcgt gggcaaaaat taccagtatt ttctggctct ggtcttcctc ataaagaatt
    32701 agctgctcag atcgcgcgac aagcaacggt actcgattca tcagatgatt tcgccgtagt
    32761 ctttgctgct attggaatca cttttgaaga agctgaattt ttcatggaag attttcgtca
    32821 aacgggcgca atcgaccgtt ctgtcatgtt tatgaattta gcaaacgacc ctgcaatcga
    32881 acgtatcgct actcctcgta tggctttgac tgctgcagaa tatcttgctt atgaaaaggg
    32941 aatgcatgtt ttggttatca tgacagacat gaccaactat gcagaggcat tgcgagagat
    33001 ttctgcagca cggcgagaag ttccaggaag aagaggatat cctgggtatc tttacacgaa
    33061 cttagctact ttattcgaac gagcaggaag aatccgcgga ctaaaaggat cggttactca
    33121 aattccgatt ctaacaatgc cagaagacga taaaactcat ccaatccctg acttgactgg
    33181 ttatattaca gaaggacaga tcatcttgac tagagagctt tacaagagtg ggattcagcc
    33241 accaattgat gtactcccgt ctctttcgcg gttaaaagat aagggaactg gacccggaaa
    33301 gacaagagaa gaccatgcag ctacgatgaa tcagcttttt gcagcctatg cacaaggaaa
    33361 acaggcaaaa gaattagccg tagttttagg tgaatctgct ttatctgatg tagacaagat
    33421 ctatgcgaag tttgctgagc ggtttgaaaa agaatatgtc aatcaaggat tctacacgaa
    33481 ccgttcgatt actgaaacac ttgatctagg gtgggaactg ctatcgatgt tgccaagaac
    33541 cgaattaaaa cgaatcaaag acgacatgct ggacaaatat ctgccagaag ggaagtgagt
    33601 tctgatggca cgattgaatg tgaatccgac ccgcatggaa ttgactcgat tgaaaaaaca
    33661 gttgacaact gccacaagag ggcataaatt attaaaagat aagcaagatg aactgatgcg
    33721 tcaattcatc ctgttgattc gaaagaacaa ccagctgcgt caagaaatgg aagagtctat
    33781 gacagaagca atgtctgatt ttgtattggc aaatgcttcg atcacggaac ctttcatcga
    33841 agaattattt gttttgccgg cggaaaacgt tgatttgtct atcgtagaca aaaatatcat
    33901 gagtgtaaaa gtacctgtga tgaattttga atatgatgaa cagctgacag acacaccttt
    33961 ggaatacggg tatttgaatt ccagtgccga actggatcgg tcaatcgatc ggtttactac
    34021 cctgcttcca aaacttttgg aactgacaga aatcgaaaaa acttgtcaac tgatggcgag
    34081 tgaaatcgaa aagacacgtc ggcgagtcaa tgcattagaa tatatgacga tccctcaatt
    34141 agaagaaacg atctattata ttcgtatgaa gctggaagaa aacgagcgcg cagaagtaac
    34201 aaggttgatc aaagtaaaaa atatgggtac cactgactaa gtggtatctt tttttaaaag
    34261 aggaaaacga tgattaaaca aagcaaacaa ataaagaaac gtttgccggc tgttcagctt
    34321 ctcgcattag gattttttct cctgatttta gttggcggcg gcctacttac cctaccattt
    34381 tttagtaata gtgggcatgg gacaaatttt atcgatgcgc ttttcactgc aacttctgct
    34441 gtctgtgtaa cgggattgac aacattgaat acagcagagc attggaacag tgctggtcaa
    34501 tttttgatca tgcttttgat tgagatcggc ggtcttggtt ttatgatggt tccgattcta
    34561 tgctttgcat tgataaagaa gaaagtcagt ctcagcacac gtattatatt aaaagaagcg
    34621 ctgaatctag aagaagtatc aggtgtgatg aagctcatgc tttacatcct aaaattggcc
    34681 tttgtgatcc aattgattgg tacggttgct ttatcttttg tatttgtgcc tgaatttggc
    34741 tggttgaaag gtatctggta cagtttgttc catgcaatct ctagtttttg taatgcaggg
    34801 tttgatttgt taggagacag tttagccaat caccagcaaa atgtttattt gatcatggtg
    34861 gtatctgctt tgatcatttc tggtgggtta ggcttcatcg tatggcgtga tttactggaa
    34921 tatcatcaga aaaagaagat gacgattcat tcaaaaatcg cattaaccgt tacaggtacc
    34981 cttttattag gcggattcgt tgtgttcttt ttcacagagc gcaatggggc aacgctagtg
    35041 gaagggaata tgtttcaacg attggcaaac acctttttta tgagtgtgac acctagaacg
    35101 gctggatatt attcagttga ttacctccat atgagccatg caggtctgat tttaacgatg
    35161 tttttgatgt acatcggcgg aacatctggg tcgacggccg gcggattgaa aacaactacg
    35221 ttaggtgtgt tgctgatcca gatgcgttcg atgttgaaag gacgtacgag agcggaagca
    35281 tttggccgta ccatccgaca aggcgcagtg ttgcgggcac ttactttatt ttttgttacg
    35341 ttgacgttgt gtgtagtcgc catcatgatt ttatcggtga ctgaaacgat acctaaaact
    35401 tctggcatcg aatatattgc ttttgaggtc ttctctgcgt ttggtacagt aggtcttacg
    35461 atgggattga cgccagactt gactgcaata gggaaattga tcattatagc gcttatgtac
    35521 attggacgag ttggtgtatt gactgtggtc ttctctctga tgacaaaagc acaaagaaca
    35581 caatcaaaat acaaatatcc agaagaaagt gtcatgattg gataatttca tcttctgaca
    35641 gaaacctagt gatttagtaa aataagaaaa taaaataatt tttcttgctt cttggatact
    35701 ttaagtgcaa aatgttcgtt tattttttga ggcactaccc gttgttgaac ctcgggtagt
    35761 gcctcttttt tattgaaagg aatcaataag atttgattcc tttatttatc aataggttat
    35821 aattcatatt tttttatttt ttcttttacg atacttagtc cagaagctaa aatttcaagg
    35881 ttattagagg atagggaaat tcgaacgaat gcatctggtt gcgcacgtgt agtaaagcgt
    35941 ctggaatgat aaagtcggat accgttagca agaaagtcat tctcaattat cttagggtca
    36001 atctcaggat caactggaag aattcgatag taagggacac gattcgttgg tttttctact
    36061 ttgaaaagag aatcaaaaag ctgattggat ctttttgtaa gacttaattt agtttctaag
    36121 atcctatttg cttcatttga atggaggatt tgagtgatga cttccacatc taatccagaa
    36181 gttttcacat ttatattaaa aattgcttgt tctatcttgc tttggacatg tactggaact
    36241 acaagataag caactcgcaa tccagaacaa ataaattttg tcattccagc taagtagatt
    36301 gtttggttag gtaataaatt ttggaaagga ggaatgatcg tatgttgata gtaagttgtt
    36361 aaaaatgaat ggatatcatc ttcaataacc catagacctt ctttttcaat cacttcggct
    36421 aattgctctc gtcttttgtg agagatttga aaccctatag gattattaca agaaggcata
    36481 agaaagacac catgaatctt cttttttcta cattcctgaa tcaatatatc aggtcgcatt
    36541 ccttgctcgt caaaatcaat tggaacgatc tctaaatgat ataactgagc caattcaatg
    36601 aaattagcgt atgtatatcg gtctgttgct attctatcac ctggagagaa aagtgcagaa
    36661 agtgtgatag ccaatccatt ttgaacaccg gaaacaatag atagagattc taaggacgga
    36721 atgactcctt gagtttttaa ccaatttgct gcagtagtca attgaaaatt tgtgcctttg
    36781 ggatcttggt aattaagcaa accaacaaga tttgggttat tactagttga ttgaataaac
    36841 gggacaacga atttattaca ttcttcaaaa gaactaacca gaccaaaatc aatgatatgt
    36901 tcggttactt cattggtaga gatggtgatg gactcaaaga cattgggaga aataaatgtg
    36961 ccacttccaa tattcgtata cagcaaacct ttctcaattc catatttata agccttgcca
    37021 acggtagtaa aatttaaatc taaataatca gctagctctc tttgggaggg taactgagtg
    37081 tttttctgta atttattctt taaaatatct tgttctaatt gttgaatcaa cgatcgataa
    37141 taaggacgct tcaatttttg tttgtctggt cgccaagtta acgggtaatt gtcataagaa
    37201 ttaataggca ttttctccac tccttaaaat tgtataccat acaattttac cattgattgt
    37261 attgatttta gaagttaatc ttttgtgagg agggaattgt ctatgaaaat tattaatttt
    37321 gaggaagtag tagcttcttt tagttttgaa gaagcgattc aagtaatgaa aaaatgtttt
    37381 tttgattatg aaagtggtaa aatcagccaa aatcctcgat tggtggaaat tttacctgac
    37441 ggaaaaggca aaaatatgtt tgcgatgatg cctgcttatt tgggggagaa cagatatttt
    37501 ggcacaaaag tcattaccgc ttttccggat aatcatcagt atcatttacc ttctcattta
    37561 ggagagattt tcttgtttga tgctcaaaac ggactaccca aagcattgat aaacgcaaat
    37621 gcagtcacct ggattcgaac agctgctgta tctgcgctgg caacagatta cttagcaaat
    37681 ccgcatgcag aaacacttgc tttaattgga gctggtcaac aagcattgtc gcatttgact
    37741 gcgatttcta ccattcgtcc catcactaca gtgtttgttt acgatatttt agaagaaaga
    37801 cgaaatactt ttatcgctaa agctaaaaaa cagtatccta aaataacgtt tataaatgca
    37861 atatcagtag aagatgcagt acgtccagcg gaaatcattt gtacgttgac ctctagtaaa
    37921 gaggcatttc ttcaaaaaga gtgggtaagg gaagacgcac atatcaatgc tgtgggcaca
    37981 tttactcctg atacaagaga gataagtaca tcgttgatgg aaacttctca aatctatgta
    38041 gatgattact cagctgcctt aacagaaagc ggggacctgc ttatagccat ggcagaagga
    38101 gatttagagg aatcttgtat tgttggtaca ttaggcgaat tagtttctga gaaaaaaaca
    38161 ttttataaag gagaatcgag gccgacgatt ttcgacgcag ttggtctagc tattgaagat
    38221 ctttgttgtg cagaatatat ttataacaaa ttagagaaag gggctttaaa atgaacttag
    38281 attattacgt agttgatgca tttgcagatg aggtgttcaa aggaaatcca gcagcggttt
    38341 atgtgctaga agaatggctg ccagaaggta cgatgcaaaa aattgccatt gaaaacaatt
    38401 tatctgaaac agcatttaca gtgaagaaaa accaagaatt tgagcttaga tggtttacgc
    38461 cagatcgaga aatcgatctt tgtggtcacg cgacattagc aacagcattt gttttattta
    38521 actattacaa aatacctgat gaaacgatta aattttctac tcaaagcggt aatctatttg
    38581 tgacaagaat taaagattat tattacatgg attttcctag tattatgccg aaaaaagtgc
    38641 cgatattgtc tgaatatgaa gaagcgattg gggctaagat taaagaagcc tacttagcac
    38701 gtgatttatt tttcgtatta gaagatgaaa aaacggtggc taaattgcag ccagatttta
    38761 cagctatcaa aaattttgaa ttaggaatag gagtcatcgt gacagcagaa agtgtgcaaa
    38821 aagactttgt ttctagaacc ttctttccaa aattaacaat caatgaagat ccagtatgtg
    38881 gatcagcaca ttctaatctt attccttatt gggcagaaag aatgaataaa acaaaattgt
    38941 cagcttatca aatgtcatca aggggcgggg atctagaatg tgaattaaaa gaagatcgtg
    39001 tgataatagg aggaaaagca gttttgtttt ctaaaggtac ctcgtttatt tgatttcttt
    39061 caaatgaatt aaaaataaat tggtggagag tggcaacctt atagctacaa tggttgttaa
    39121 aaaaagacgc aaactttttc aatttaatga aaagtttgcg tctcatttta attattggtg
    39181 tttgtttttt attcaatcaa gagtaaaatg ccccatacta actatcctcg gatgacttcg
    39241 attttataac catcagggtc aatgacaaag tagtaagaag gcgcagtgcc aggtaatcct
    39301 tttaagtcag taatctcaag accagcagct ttatgttgct cgtgtaattt ttctagatca
    39361 tcagctgcta tagcgatatg accatagccg tctcctagat catacgcacc atgatcatag
    39421 ttgtaagtca actctaattc atagtcatcc ccaggaagtg tcaaataaac aagtgtaaat
    39481 ttgttttctg gaaaatcacg gcggcggctt tcttcaaaac caaatgcctt ttgataaaat
    39541 tcgattgatg cttcaagatc tttcactcga acacatgtgt gagccatttt catagattca
    39601 tctctccttt tccttttact caatcataac aaaaagaagt aaggaaatag ttgatttgtg
    39661 cttgttcatg agaaatttct catgtaaaat ggatagaaaa taaaactgtt aaggtggaaa
    39721 ctattcatgg ataaaccaat ttttggtgaa aaaaagcaat ttgctgatta tcaaacccgt
    39781 tatgcagcat atattgtcat agaaagagat cagcagattg cagtaatcga ggctccaaat
    39841 ggtgcttttt ttcttcctgg cggggaaatt gaaggagaag agacgaaaga agaagcgatt
    39901 gtccgtgagc tgatagaaga aatgggaatt gtggcagaaa tttcttctta tctgggagaa
    39961 gctgatgagt atttttactc gaattatcgt gcaacttatt attataatcc aggatatttc
    40021 tttgttgctg attcttggaa gcgtatagga gaaccaactg aaaagaccaa taaaatttgg
    40081 tgggtgagcc ctcaagaagc gcttgtgaaa ttgaaacgtg gtagccaccg atgggcagtc
    40141 gagagatgga tcgaaaaaaa taaaaactga aagaaagcga gtagatcagc tttctctcag
    40201 tttttttatt tattacagta catatttttt gattgcttca gctacaccgt gctcgtcgtt
    40261 tgatgaagtg atgacatttg ctgcttcttt gacaagagaa acggcatttc ccattgctac
    40321 tcctagacca gcatattcaa tcatggataa gtcgttctcg ttatctccga tagccatgat
    40381 ttcatcttga tcaataccaa ggtgctgtgc gagattcgca actgccgcac ctttacttgc
    40441 ttctttattt agtacttcga agtaataggg cgcacttttg accgttgtgt atttatcacg
    40501 gaaagtttgc ggtaaacgcg caattgctgc atctaaaatt tctggttcgt cgatcatcat
    40561 cattttgacg atgttcatgt ctggtgtcat ttcttcaggt gttcgatatt ttagcggcat
    40621 cttgactaaa cttgcttcgt ggatcgtata agcgctgata tctcgattag gcgtgtagat
    40681 cgctttgtca tcgatcgaat ggagatgact gccgacacgg cgtgccatta cttcgatctc
    40741 taggaaatct tcatgtgtca gtccgtgacg ggcgatgatt ttacctgatt tcgtttgttg
    40801 tacgagtgcc ccgttataag taataacgta atcattatct tgaaaaaggt tcaactcgtt
    40861 taactgttcc tggacacctg gtaagggacg gccagtgcat aaaacgatcg aaacaccttt
    40921 tgcaacagca gcggcaatcg cttcttttac ttcttttgtt acttctcttt tctcatttaa
    40981 taaagtacca tcgatgtcga tagcgactag tttgatagac ataatttcct ccaatatatt
    41041 ttaatttatt tcaatcaatg cgccattacg gatatggctg gcaaattctt gataagtttc
    41101 gtcaaataaa tcataatgat cacgcaagga cggatcaacc atttcttttg gaaagaaaaa
    41161 tcgctcatct ccttgttctt gtcccgctaa tgcagcaaca agctgactaa cctgtgataa
    41221 ttctatcaaa gttccgtcat ttcgcaaaat ctcgatttgt gtgcgatgtc tgccttgttc
    41281 tgggcgataa aaatcataag gtagatcgta gctagaattc acagcagtgt aataaacagg
    41341 attatatccg accttttcta ctaacaaagt gagttcttgg actaattttg aatcccggtt
    41401 gccagaaaaa gtagcagatt tcagtggttt acgattcaaa aaacgtttag ctaaatcatt
    41461 taaaatagga tcagcagatt cagaccattg agtaaagtaa gtgcccaaga ccccatcatc
    41521 aagtttgagg tattcttcta aagtaaagct tccgtcaaga aatggcagaa gtaattgaga
    41581 atggagttca aaactgctgg catcttcttg atacaactcg tgtgcgcgat gaagcagatg
    41641 ctccaaaatt acttccatac cacgggaaac aggatggaag tacacttgca catacatttg
    41701 atatcggcta acgatataat cttccactgc atgcatgcca ctcatggaaa aagcgatccc
    41761 gccttcataa ggacgaatca cacgtaatat tcgggtaaga tcaaatgttc catattctgt
    41821 tccagtgaaa taagcatccc gaagcaaata gtccattcga tctgcatcga tctgactaga
    41881 aatcatttgg acaacttgtg ggttggggta agttttttgg atcacactag ctaccttttc
    41941 aggaaaacct tcttctactc ggttaagaat ctgatacact tctgtttctg gagaagtgat
    42001 gatctctaca gtgatggctt catgattcgt atggaagata tgttcaaatg tatgagagta
    42061 aggaccatga ccgacatcat gcaacagtgc cgcacataaa gtcaccagcc gttcctgatc
    42121 atcccagcct ccgttgccta ttttttcttt tgaaaaattt cgagaaaaga tatcgcagat
    42181 tcgtcttgaa atctcataaa cgcctaatga atgagtaaaa cggctatgtt ctgctccgtg
    42241 aaaagtgaaa gaagaagtgc ctaattgttt aatgcgtcgt agacgttgga cttctttcga
    42301 attgatcaaa tctaagatta cttgatgctg gacatggata tagttatgga ctggatcacg
    42361 aaaaactttt tccataggca gcatttgata tttataaggg gttgtcataa tgtagctcct
    42421 ttatgttatt acgttgttcc attctctgga tttggttgtt ccattgctcc agctggattt
    42481 ctttttttag ccagtcggta gaatcctgta aatcacttgc agaatacagc ttttcaaaag
    42541 cttttgcaaa tgcttctttg acttctttta caaaaagggg ttggtcaagc agttcttcaa
    42601 gtgttgccat tgtagcagga tccactgcag gatagccatt tgtaccaaag tcttctttta
    42661 aagcagattg ataaaatcgc ttcatgactt gcccacgttt ttcctgatta ccgttgacac
    42721 ttaagtacat cataacagaa atcccatttt tgatccgacg ctgagcgata ccggcaaatt
    42781 ttttgccatg gatgcttaaa tcataagttc caggacaata agaatgggtg atttcgaatg
    42841 cttgtatcgg tgcctttgga aaagcgtatt tagtcaatag gagcatttgc tcgtaagcgt
    42901 catctatggt tatttttttt gtgtcacttt gggggaaaat caaagaaata ttcaaaattc
    42961 cttcatctga tacgatccct aatccacctg aatttctgat cactggagcg taacctgcat
    43021 cttccagtgt ttggatcccg tcacttagaa aaggagttcg aacatccttc atacctaaga
    43081 tgactgtgca gtcatattgc cagaaatgta aaaagatttg ctggtgttct ttagaatatt
    43141 ccgttaatat atctgttaat gcaaaagggg aaaagtcgtt tttttgataa atgtgctgat
    43201 ctagtcgaat cgcagggttc attcaaccgg ctcctttgta taaattcata ttttctacaa
    43261 tattatactc taaaatcaaa gagttgtttt ggaaaaaagg ttttctgtaa aaatggacaa
    43321 gttggttgac aaaacaaaag aaactaggaa caatgtagct atgagaagta aaggagaatg
    43381 aataatggaa ttatcacaag gaacaccgat ttcaatcaaa ttgcgtacaa aagtaaaaca
    43441 gcaaggggaa gtgcaagatt tttattttga tttgaaaggg caaatggtga caattggcga
    43501 tacgctgtac atacgttata aagagattca ggaggagacg ggcgatgaga ttcctgtaac
    43561 gatcaaatta gttccagatg gtcatgtaca gctgatccgt gcaggagaaa tgcggatgcg
    43621 tttgaaattt ggctataaag aacggttaga aacatcctat cgaacaccat acggtatgat
    43681 ccagattgct acttttacga aagaactgca tgttagtttg aaagaccgtc ccacagcagg
    43741 gaaggttcga atcgattatg atctgttcat gggaccggaa aaaatcggcg aatattattt
    43801 gaccttggat tttacagcgt aaatataaaa gaaaagtttg attttttgtg tctgattttg
    43861 tatgataagg agtagactgt gaaaggacgt gtcctgattg gaaattagtg tatttgaagg
    43921 tgcaaacaaa agcgaattat caatgatcga agtagcacac gcgatcttag aacaacgtgg
    43981 cgatgtcatg gatttttctg atttagctaa ccagatccaa aactatcttg ggaaatcaga
    44041 cagtgaaatt cgtgattcac tcgcacaatt ttatactgac ttgaatattg acggcagctt
    44101 tatctcatta ggggataatc gttggggctt gcgttcatgg tatgctatcg attcgatcga
    44161 tgaagaagta aaccatggaa tggacgaaga ggatgaagat actccacgtc gccgtaaacg
    44221 caagaaagtc aacgccttta tcaatgatga tgaagacgcg atcgattaca atgacgatga
    44281 cccagaagat acagatctga cagaagaaga tgacgatgat ctgtttgatg acgatgatga
    44341 tgaagatgaa gaaatcgctg cttataattc tgacttgcaa gaaatcggag cagatgaccc
    44401 gacagatgat gaagaagact tgccaggaaa tatcgaagaa gatttaagta tcatagacga
    44461 agacgatgat gatgaagatt atgaagagga agaattttct gactctgaaa aagaataaac
    44521 attattttct tacgagaggt tgtgacagag acgatcagcc tatcagaata aggaggtgtg
    44581 acacgatttt t
//
LOCUS       DACDEG010000014        43238 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.014, whole genome shotgun sequence.
ACCESSION   DACDEG010000014 DACDEG010000000
VERSION     DACDEG010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.014"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            616..1305
                     /locus_tag="IV142_000850"
     CDS             616..1305
                     /locus_tag="IV142_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8484525.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1292..2500
                     /locus_tag="IV142_000851"
     CDS             1292..2500
                     /locus_tag="IV142_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8484526.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2794..4065
                     /gene="serS"
                     /locus_tag="IV142_000852"
     CDS             2794..4065
                     /gene="serS"
                     /locus_tag="IV142_000852"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP8484527.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4614..6098)
                     /gene="guaB"
                     /locus_tag="IV142_000853"
     CDS             complement(4614..6098)
                     /gene="guaB"
                     /locus_tag="IV142_000853"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP8484528.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6603..7298)
                     /locus_tag="IV142_000854"
     CDS             complement(6603..7298)
                     /locus_tag="IV142_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP8484529.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7388..8488)
                     /gene="ychF"
                     /locus_tag="IV142_000855"
     CDS             complement(7388..8488)
                     /gene="ychF"
                     /locus_tag="IV142_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP8484530.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8497..8688)
                     /locus_tag="IV142_000856"
     CDS             complement(8497..8688)
                     /locus_tag="IV142_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP8484531.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8709..9599)
                     /locus_tag="IV142_000857"
     CDS             complement(8709..9599)
                     /locus_tag="IV142_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP8484532.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9586..10353)
                     /locus_tag="IV142_000858"
     CDS             complement(9586..10353)
                     /locus_tag="IV142_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8484533.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10365..11078)
                     /gene="rsmG"
                     /locus_tag="IV142_000859"
     CDS             complement(10365..11078)
                     /gene="rsmG"
                     /locus_tag="IV142_000859"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP8484534.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11310..13211)
                     /gene="mnmG"
                     /locus_tag="IV142_000860"
     CDS             complement(11310..13211)
                     /gene="mnmG"
                     /locus_tag="IV142_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP8484535.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13228..14625)
                     /gene="mnmE"
                     /locus_tag="IV142_000861"
     CDS             complement(13228..14625)
                     /gene="mnmE"
                     /locus_tag="IV142_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP8484536.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14969..15307
                     /locus_tag="IV142_000862"
     CDS             14969..15307
                     /locus_tag="IV142_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484537.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15475..16233)
                     /locus_tag="IV142_000863"
     CDS             complement(15475..16233)
                     /locus_tag="IV142_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP8484538.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16251..17069)
                     /locus_tag="IV142_000864"
     CDS             complement(16251..17069)
                     /locus_tag="IV142_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP8484539.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17066..17422)
                     /gene="rnpA"
                     /locus_tag="IV142_000865"
     CDS             complement(17066..17422)
                     /gene="rnpA"
                     /locus_tag="IV142_000865"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP8484540.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17540..17674)
                     /gene="rpmH"
                     /locus_tag="IV142_000866"
     CDS             complement(17540..17674)
                     /gene="rpmH"
                     /locus_tag="IV142_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP8484541.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18197..19531
                     /gene="dnaA"
                     /locus_tag="IV142_000867"
     CDS             18197..19531
                     /gene="dnaA"
                     /locus_tag="IV142_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP8484542.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19729..20859
                     /gene="dnaN"
                     /locus_tag="IV142_000868"
     CDS             19729..20859
                     /gene="dnaN"
                     /locus_tag="IV142_000868"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP8484543.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21088..21330
                     /gene="yaaA"
                     /locus_tag="IV142_000869"
     CDS             21088..21330
                     /gene="yaaA"
                     /locus_tag="IV142_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP8484544.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21317..22441
                     /gene="recF"
                     /locus_tag="IV142_000870"
     CDS             21317..22441
                     /gene="recF"
                     /locus_tag="IV142_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP8484545.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22438..24384
                     /gene="gyrB"
                     /locus_tag="IV142_000871"
     CDS             22438..24384
                     /gene="gyrB"
                     /locus_tag="IV142_000871"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP8484546.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24404..26875
                     /gene="gyrA"
                     /locus_tag="IV142_000872"
     CDS             24404..26875
                     /gene="gyrA"
                     /locus_tag="IV142_000872"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP8484547.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27111..27410
                     /locus_tag="IV142_000873"
     CDS             27111..27410
                     /locus_tag="IV142_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP8484548.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27460..27972
                     /gene="ssb"
                     /locus_tag="IV142_000874"
     CDS             27460..27972
                     /gene="ssb"
                     /locus_tag="IV142_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8484549.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27998..28234
                     /gene="rpsR"
                     /locus_tag="IV142_000875"
     CDS             27998..28234
                     /gene="rpsR"
                     /locus_tag="IV142_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP8484550.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28377..30350
                     /locus_tag="IV142_000876"
     CDS             28377..30350
                     /locus_tag="IV142_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP8484551.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30356..30808
                     /gene="rplI"
                     /locus_tag="IV142_000877"
     CDS             30356..30808
                     /gene="rplI"
                     /locus_tag="IV142_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP8484552.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31082..32449
                     /gene="dnaB"
                     /locus_tag="IV142_000878"
     CDS             31082..32449
                     /gene="dnaB"
                     /locus_tag="IV142_000878"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP8484553.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33229..34521
                     /locus_tag="IV142_000879"
     CDS             33229..34521
                     /locus_tag="IV142_000879"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP8484554.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35308..36171
                     /locus_tag="IV142_000880"
     CDS             35308..36171
                     /locus_tag="IV142_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8484555.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36300..37922)
                     /locus_tag="IV142_000881"
     CDS             complement(36300..37922)
                     /locus_tag="IV142_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8484556.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            38171..38692
                     /locus_tag="IV142_000882"
     CDS             38171..38692
                     /locus_tag="IV142_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484557.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38878..40608)
                     /gene="spxB"
                     /locus_tag="IV142_000883"
     CDS             complement(38878..40608)
                     /gene="spxB"
                     /locus_tag="IV142_000883"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP8484558.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40773..41591
                     /locus_tag="IV142_000884"
     CDS             40773..41591
                     /locus_tag="IV142_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP8484559.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41694..41891)
                     /locus_tag="IV142_000885"
     CDS             complement(41694..41891)
                     /locus_tag="IV142_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484560.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            42060..42638
                     /locus_tag="IV142_000886"
     CDS             42060..42638
                     /locus_tag="IV142_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP8484561.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
BASE COUNT        13150 a         8278 c         8531 g        13279 t
ORIGIN      
        1 cagttttcaa aggtctactt catcaggagt gtttcccgat tcgtttttgg ctgccgtagc
       61 aacttttata tcatatcaga gagacaagta attgtcaact cttttttaaa agttttttcg
      121 atttcgtttt tcgaaatctt ccagcaactc atttatcata acttatcgtt tgttatttgt
      181 caagaacttt tttgaagttt ttttcgatag aatcaagctt cgcaagtggt tcttttttaa
      241 cgactcagtt atcttatcat gtcatttcca acatgtcaac aacttatttt ttattattta
      301 ataagttatt ggtgttcttt tgaacaaata ataatatatc aacatcacat gtgaaagtca
      361 ataagaaaat gcttatttat caacatttct agcttacatg atcattggtt agttctggtc
      421 ctttttcgtt cgtctttata actccgagag acgtaaaatc cttttgtttt cagtttaatg
      481 ttcatgttct tgtcttattc acaccaacta gatgtctcac cttctaaaat tttccttaac
      541 aaagtgctgc ttttatctat ttctgctttt ttttgctaga ataatagtaa caatatgact
      601 ctggaggatt tatttatgaa aattttgatt gctgacgatg ataaagaaat cgttgaatta
      661 ttaagtatct atgttcacaa tgaggggtat gaagcagtaa aggcttatga tgggaaagaa
      721 gcattatcta aaatcagaac aatccctgat attgaattac ttattttaga tatcatgatg
      781 cctgagatgg atgggatgca agtagtgaaa gagctacgaa aagaatccca aataccaatc
      841 attatgctga cagcaaaaac tactgatatg gataagatca aaggactggt cgctggtgca
      901 gatgattatg tgaccaagcc atttaatccg ttagaagtaa tggctagagt aaagtcttta
      961 ttacggcgta caaaaatgca gattgcccca gatcagcctg atgtattaga agtaaatgca
     1021 ttaatcatca ataaagactc tcatgaagtt aaaacaatcg atgggaaaga aatccagtta
     1081 acagctttgg aattcggtat cctctacttg cttgctagtc atcctaatcg agtatttagc
     1141 gcggatgaaa tttttgagcg tgtttggaaa caagaaagta ttgtctctgc taaaactgtc
     1201 atggttcacg ttagtcattt aagagataag attgaagaag caactggtgg tgaaaaagtc
     1261 attcaaaccg tttggggagt gggctataaa attgacgcaa gatagtaaac gagcaactga
     1321 aaaacgccgt cggatcaatt tgacttcaaa agaaatcagc gaattgttag cggaaggaat
     1381 catcacgatt atccttttgc ttctattaaa tgtttctatt ctggttgtta tcagttcggt
     1441 gatcaacagt tcaccttctt tgacgaatgc gatttgggat tcaaagaata tttttgcaga
     1501 acgattgaac actgatttgt tctggaacgg ccggaatttt attattccct ttttctttct
     1561 attagatatt ggtgttttgt attggcgctt gattcgaaga tatcgccaaa tgcaattgcg
     1621 gcatatcatc agcgaacttc actacatcgc aaacggaaat tatgatcatc gtatcccttt
     1681 tgaactgagt ggtgacttaa gccgagtggt aacaagtatc aatggattgg tggatagtac
     1741 cgtagcagcg attgaagacg aacgtaaaat cgaaaaatca aaagatgaat tgatcacgaa
     1801 tgtcagtcat gatatccgta ccccactcac gtccatcatt ggatacctag gactgataga
     1861 agatggccag tatcactcag aagaagatct tttaaagtat acgcatactg cttatatcaa
     1921 agcaaagcag atgaaatctt tagtggacga cttatttgaa tacactaagg tacgccagcc
     1981 tgctgtcccg gtgaattttt ctgcctttga catgatccag ttgatcgaac agttagcagc
     2041 tgattttgag ttggaagctt ctaaaaaaaa tatccaaata cttgttcaat caaaagtaga
     2101 ctcccttatc atggatggcg ataccgaaaa acttgtacgt gtcttcaata atcttttgac
     2161 gaatgcgttg aaatacggaa aaggagcaac gaagatcgta atcgaagtag aacgtatcgg
     2221 ttcagaagtt gttgcaacag tcaaaaataa cggagcaatg atcccacaac aggcaatcga
     2281 taatctattc gaccgttttt atcgtgtaga agaatctcgt tcacaagcaa ctggaggaac
     2341 tggacttggt ttagcaatcg ctcaaagcat tgtggctttg cacggcggat acatttatgc
     2401 aaaatcagat aagcaatgga cttctttcat catgcacctt ccaatcaaga aaaatgaaaa
     2461 attacctatt gatacacagg aagttattga cgaaagctga aatattcgct attcttatta
     2521 caattattag aataaatgaa aaacacagaa cccagtacac tttattcttt ttcagagagc
     2581 tgatggcagg tgtaaatcag caaaggataa tttggaagtt ccagttcgtt tacgtgccct
     2641 ttcgaaataa gaaaggtcgg ttcaacagcc gttatgtgtt caacaagtgc agctgacgct
     2701 gaacttgggt ggtaccgcga atttcagact gattcgtccc aagaataatg ggatggatca
     2761 gtcttttttt attttcaaaa aggaggaaat catatgttag atgtaaaaat gatgcgacaa
     2821 aatattgaag aagtacaaga aaaattgatg actcggggcg taaaaaaaga aacattagct
     2881 gaattcatgg aactagatga aaatcgtcgt aagctactag tcaaaagtga agaattaaaa
     2941 aaatatcgaa atgatgtttc tgctgagatc gcccaattga agcgtgcaaa agaagatgct
     3001 tctgctaaaa tcgctgaaat gaaagaagtc ggtggcaaca tcaaagcatt ggatactgaa
     3061 attgctgaag tggatgaaaa attgaaacaa attgcaacta ctttgccaaa ccttccccat
     3121 gattctgtgc cagttggaac agatgaagag gacaatgtag aaatccgtcg ttggagcgaa
     3181 ccacgtacat ttgcttttga accaaaacca cactgggaaa ttgcagaaaa tcttgatatt
     3241 ttagattttg aacgtggcgc aaaagtagcc ggtagccgtt ttgtctatta caaaggatta
     3301 ggtgctcgat tagaacgtgc tgtctacaac ttcatgcttg atcagcatat ttacgaacac
     3361 ggctatactg aaatgatcac tccttatatc gtcaacagta aagcgatgtt tggtacggga
     3421 caatttccaa aattcaaaga agatgttttc caattggaga atactgatct aacattaatc
     3481 ccaacagctg aagtaccatt aactaactat tataatgatg aaattttaga tggtgcagat
     3541 ttacctattt atttcactgc actaagccct gcttttcgtt ctgaagccgg aagtgctggt
     3601 cgggatacac gaggactgat tcgtcttcac caattcaata aagtagaaat ggtcaaattt
     3661 tcggatgtag agcattctta cgaagaattg gaaaaaatga cagctgatgc agaagatatc
     3721 ttgaaaaaat tgcatttgcc ttatcgtgtg atggccttat caactggaga catgggcttt
     3781 tctgcagcta aaacttatga cttagaagta tggatacctg cacaagatac ttatcgtgaa
     3841 atcagttctt gttctaactg tgaagatttc caagcacgcc gcgcaatgat ccgttatcgc
     3901 gataatgatg gaaaaatcca ctatactcat actctgaatg gttcaggact tgcagttggc
     3961 cgtacagtag ctgccatttt ggaaaactac caaaatgaag atggttctgt cacagttcca
     4021 gaagcgctcg ttccttatat ggggaatctg aaagttatta aataaagagg ctgtgaaaaa
     4081 gccgcttagt ttcgagtatt aagaaaaatt ttgtgaaaat ggctttccac atttttgcat
     4141 aaattggact taataccgag aaactggctt ttgaacacca tttatcgaga ggttgtgaaa
     4201 tcagcgaatt aacctgagta ttagagaaaa aaatggaaaa atagtccctc atattttacc
     4261 gtttgttgaa ctaataccga gggtcagctg attgaacacc gtttagtgga gaggtcgtaa
     4321 gcctaccccc cagctcctag ttacaaggac aaattcaaaa aacagctctt catattttat
     4381 gaatgttgta cttgtgattc ggaggtggca agggaacact gtttattcaa aaagaggctg
     4441 ggacagaagc gtttaactcc aagaaataag aagaaattca cgaaaattgc ttttcaaatt
     4501 tttgtgaatt tcttcttatt tccgaaggag ttgcttctac ttccgccgtt tatacgtgtt
     4561 tagagcgtga gacaaaagtg ttctttactt ttgtcccacg ctctttaatg tttttaagat
     4621 tcaactgaat agtttggtgc ttcttttgtg atttgtacat catgagggtg tgattctttt
     4681 aatccactgc cgctcatttg gataaattgc gcttcatcac gtaattgctg tagattggct
     4741 gccccaacat aacccattcc tgaacgcaaa ccgccgatca tttggaaaat aatatctgca
     4801 acacttcctt tataagcaac ccgaccttcg attccttctg gtactaattt atttgcttca
     4861 ttgacactac cttggaagta acggtctttt gaaccttttt ccatcgcgcc taatgagccc
     4921 atacctcggt atgttttgaa tcgacgtcct tggaagattt cgaattcacc tggtgattca
     4981 tctgtccctg ccaacatact tcctagcatc actgcatgtc cgccggcagc taatgctttg
     5041 acgatgtcac cagaatattt gatccctccg tctgcaataa tcgctttgcc atattgacga
     5101 gcaactgatg ctgcatcata aatagctgtg agctgaggta cacctacacc ggctaccaca
     5161 cgtgtcgtac aaatcgatcc tggtccgata ccaactttta cgacatctac acctgcatca
     5221 tataaagctt ttgtcgcttc agccgtcgca acgtttcctg caatcaatgt tgcatctgca
     5281 aaagttgaac ggatttcttg aatttttcgg atcacacctg cactatgtcc atgagctgta
     5341 tcgatgatga ttgcatctgc ccctgcttct aataaagcat tcgcacgctc aaatgtatcg
     5401 cttgttacac caacggctgc tgctacaagc aatcgaccat gttcatcttt tgctgcattt
     5461 ggaaattcga tcactttctc gatgtctttg atcgtgatca atccgcttaa acgaccttcg
     5521 ttatctacga ttggtaattt ttcgatttta tgtttttgca aaatcttttc tgcatctttt
     5581 agagaggtac ctactggggc agtcactaag ttgtcttttg tcatcacatc agcaatcggc
     5641 atgtgataat ctgttacaaa acgcatgtcg cggtttgtaa ttatccctac caattttctg
     5701 ttttcaagtg tttcaacgat tggcacacca ctgatacgat atttgctcat caattcttct
     5761 gcgtctgcta caagatgctg tggcgttaag aagaacggat caatgattac gccactttca
     5821 gaacgtttga cttttcttac ttcatccgct tgctggctga tcgtcatgtt tttatggatc
     5881 acacctagtc cgccttgtct tgccatagca atggccatct tgctgtcggt aacagtatcc
     5941 atacttgcac tgatgattgg aatattcaac ttaatatttt tagctaattg tacgctcata
     6001 tccacttcgt ttggtaacac gtgactttct gctggtatca ataagacatc atcaaaagta
     6061 aatccttttt tcgtgaactt tgtttcccag ttggacattt ttagtaccac tcctctaatt
     6121 tagtttatct attaaaataa ttgatttttt catttaagtc aatgatctac ttggaatcaa
     6181 gttagctttc tcatagaata ccaatataaa gcgtttacat ctgtgtgtat taaaagagat
     6241 tgtaaaaaag ctgtcctgca tcaagtaata agaacagcca atcaaaaaca gcttctcatg
     6301 tttttgaatt ggttgggttt attaccgcag atgcaaactt ttgaacaccg tttagtggag
     6361 aggctgtgaa aaagctgctt agtttcgagt agtaagaaaa attttgtgaa aatggctttc
     6421 cacatttttg cataaattgg acttaatacc gagaaactgg cttttgaaca ccgtttatcg
     6481 taaaaaggga ctgtgacaag acttttgtca cagtccctta ctattattta actatggata
     6541 aacaaaatga ggttctttac tacccctaga tatctagcaa aatagctgat ctttttgcta
     6601 gtttatcttc ttgggacgga taaacttcca ataatcccgt attttttctt caagtagtaa
     6661 cggattgcta aagcagttgc cccgataatg ataactacta ctggatctaa tactggattg
     6721 ataactggtg gcaatagtgt tgtcgcagaa aacagtacga cccaaagaag tgtcgttcca
     6781 cctaggataa gcaatgtctt acccattcct ggtttcttgc ttcgatctgc tccgggatat
     6841 tcatattgat agatgtattt gtacatcata taaaagaccc agccaccgat tgcagaacct
     6901 agaacgagtg tagaaaagcc ataagctggc gttgttccac gtgaaaacaa tccggtaatc
     6961 gcaatcatga tacttaatag accaagcaac agcaacgtat tatctaacca catcaataaa
     7021 ggggtggatg aattttttac ttctgctggc ttattgatga ttgcttccgt tctttcagag
     7081 actgtcccaa acaattgacg ggctgttctt ccgccttttt gttctgcaac taaagtaggc
     7141 agaatgtcat gaagggcttt tactttttct tcttcagaca aattcgctgc ttctaaagat
     7201 tttttcaaat caaaaatgta ctgttgattt cttttggtta attttgtttc taaactattg
     7261 ttttctttta cgattgctcg taattgttct ggttccatta tctaatagtc ctccttagcc
     7321 gttccttttc tcaacggtcg tcacaaaaga cattctacca tattctacag ctagtttgat
     7381 agccctttta cacattaaag cggaataaca ttacgtcgcc gtcttgtacg acataatctt
     7441 ttccttctaa tcgaacacgt ccagcttctt ttgctgcatg catgtttccg taatgatcca
     7501 agtcttcaaa agagacagtt tctgcacgga taaatccgcg ttcgaaatct gaatggatga
     7561 ttcctgctgc ttgtggtgct ttgattcctt ttttgaatgt ccaagcacgt acttcttgtt
     7621 ccccagcagt aaagtaagta gctaagccta acaaatcata agcagcacgg atcaactgat
     7681 ccaaacctga ttcttcgatt cctaaagctt ctaaaaattc cgctttgtct tcttcatcta
     7741 attctgcgat ttcttcttct gcacgagcac tgaccacgat cacttctgca ttttcttctg
     7801 cagcaaagtt gcgcacttct tgtacataat gattcgcgtc tgcatctgct acgtcatctt
     7861 ctgagacatt ggctacataa agaattggtt ttgtcgtcaa taaaaatagt gatttaacga
     7921 ttttttgttc ttcttcagta aattcgatcg ttcgggcaga ttttccttct tccagtacag
     7981 gtttgatttt atccagtaca gctagttctg ccactgcatc tttatctttg gttttagcaa
     8041 cttttgctac acgagtatag cgtttgttga tagattctaa gtctgctaac actagttcca
     8101 agttgatcgt atcaatatct tctagtggat caacacgtcc ttctacgtga gtaatattat
     8161 catcatcaaa acagcgaaca acgtgacaaa tagcgtctac ttggcggata tggcttaaga
     8221 attgatttcc caaaccttct cctttacttg cgccttttac gattccagca atatctgtaa
     8281 attcaaaagt cgtcggaaca gtttttttcg gatgtacaag ttctgttaag cgttgcaaac
     8341 gccagtcagg tacttctacc atccctacgt tggggtcgat cgtcgcaaag ggatagtttg
     8401 ctgcttctgc tcctgctttt gtgatcgcat taaacaaagt tgattttcct acgttgggca
     8461 acccaacaat tccagcagtc aatgccataa atttattcac tctttctctg catttttttc
     8521 aattactttt tttaatttct tttcaaaatc tcgacgggtc atcatgacga tatgtccgca
     8581 attcatacat ctgattttga tatctgcgcc cattcgaatg acttcccagc gattcgtctg
     8641 gcaagcgtga ggttttttca tttcaacgat atcgtgcaag tcgtacataa aaatagcctc
     8701 ctttttgttt attcttcatc aaattgaata ttcaagatat caagaattcg agtcaaatct
     8761 ttttgagaaa gatattcaat ctcgatctta cctttcccat ttttttcttg gatagctaca
     8821 cttgtaccaa atttatccat caaacgatct tcgctttctc ggatataata tggtttttcc
     8881 tttgctagcc ttgggatttt tttcttttct tttccatcat tttgatttaa ctcagaaaca
     8941 agctgttcca gttggcggac agttaaattt tcagttactg cacgattggc taatttcaag
     9001 atcaatgttt tattttttaa acctaacagc gtacgggctt gtcccataga taaacgctga
     9061 tcttgtacca tttccttgac aagatccggt aaagaaagca agcgcaaata attggcgata
     9121 taaggtctgc tctttcctaa acgactagct acttctgctt gtgttagttt caggtttttc
     9181 atcagcatct catatgcttc tgcttcttct aacggattca gatcttctcg ctgcaagttt
     9241 tccagcactg ctacttgcat catcgcttct tcgtcaaagt ctctaacgat agcagggatc
     9301 gtttcttttt cagctagttt ggacgctcga aaacgtcgct cccctgcaat aatctcatat
     9361 ccttttactg cggaacggcg tacgatgatg ggttgaaaga cacctgattg ttcaatagaa
     9421 ttagctagtt cctgcaaaga tacttcttca aatgttttac ggggctgata cggatttggt
     9481 cgcagctcat tcaatggtag ctggacaacg gtatcatctt ttacatccac cgtctctaag
     9541 ttttctaaat cttgaaacag tgcatcgatt cctcggccta atcctttact tttcttcacg
     9601 acttaccact tcctttgcca atgcttggta gacttcagct cctcgagaac gtggatcgta
     9661 gtcgatgatc ggttttccat gactaggtgc ttctgataaa cgaatatttc ttggaatgat
     9721 cgtttcgtaa actttttcgc ggaagtattt acgtacttct tctacgactt catttcccag
     9781 atttgttcgt gcatcataca ttgtcagtaa gactccctcg atctctaatt ctggattaaa
     9841 gtgtttttgg actaaacgaa ccgtattcag taattggctt aatccttcca atgcgtaata
     9901 ctcacactga acaggtatca aaatcgaatc acttgctgta aatgaattga ttgtcagatg
     9961 gccaagagaa ggcgggcaat cgatcaaaat ataatcatac tgactgctta cttctgctaa
    10021 agatcctttt aatcttgatt ctcttgccat cattgacgtc aattcgatct ctgctcctgc
    10081 taattggaga gtcgcaggca caatactcaa attttcgtgt tcagtgatca aagttgcctc
    10141 atcaattggc aattcattga ctagaacatc atagatgtct cttgtcacat caggttttct
    10201 gatcccgaca ccactggtag catttccttg tgcatccata tccactagca atactttttt
    10261 gccaaggcta gcaagacaag cacctagatt caccgtcgtc gttgttttgc cgacgccacc
    10321 tttttggttc gcaacagata ttatttgtgc catctcttct cctcctattt gattggctgc
    10381 ttattaggca gtccgggctt tcttgggtat ttctttgggg ttggtttctt tttttgaata
    10441 acgatgatat gccgttgatc agagacattc ggcaaggtaa ccgcctcttc tttgaggaat
    10501 ttaccaccta ataaagcaat cgcttgtttt gcttctgtca attcctcttc actttttgct
    10561 gctttcaatg cataaaaata gccttcttgt ttagccaagg gcaaacacaa ttccgctaac
    10621 acattcaaac gagcaaccgc acgagcagtg acaaggtcaa atgatgcacg aaagtgagga
    10681 ttttgcccaa atgtttctgc ccggtcatga tataaggaag catcgattcc taataatcgg
    10741 gttaactcag ataagaaggt tattcgttta ttcagagaat caacgatcgt tatctgcagt
    10801 tctggaaagg caatttttaa aggtatgcta ggaaaccctg cccccgaacc aacatcgcat
    10861 aaagataaag gttgcgtaaa gtctacttca aatgctaatg taagtgaatc gtaaaaatgc
    10921 ttcagatata cgtctttttc atccgtaata gctgtaaggt tcatcttttc gttccattcg
    10981 actaatgtcc gataataaat agcgaattga ttcatttgat gatcggtaag ctctatccct
    11041 ttttctgcca aggattggcg aaattcttct ggtatcataa atattccttt cattgtgaaa
    11101 cagcaattgt ttcacagatt ctctccctac tttaacgcaa aatctctaga catgcaatcc
    11161 ttccatatgg attcttaaaa atttgaaaag taagaaaagc cgatagactt tttctttcta
    11221 gtatgactca taaaatatac acagaagaga gaactagctt tgagcaattt ctgcaaaaac
    11281 tagttctctc ttctatttat tattacgtat tacgctggtt cactgacttt cgcaatcttc
    11341 ccttgttcga tatagaccat taggatactt aggtctgcag ggtttacgcc actgattcgg
    11401 ctagcttggg caattgtttc tggttggatt ttctgcaatt tttgttttgc ttctgttgct
    11461 aatccgttga ttgctgcgta gtcgatgttt tctggaatct ttttcgcttc catgcgtttt
    11521 aatttttcta ctttttccat ggcttttttg atgtagcctt catatttgat ttggatttct
    11581 acttgttcga tttcttttcg tgtcaattct tcattttctg gaataaaccg tagaagttct
    11641 gcataagtaa tctcaggacg tcttaaaaag tcgcttgcta gtacaccatc ttttaatggt
    11701 gccgcattaa tcgttgctaa aaagtcttgg acttcttctg ttggtttgat tcgtacattc
    11761 atcaaacgtt tgatttcttg ttcaactgct tcttttttct tcaaataatt cttgtattga
    11821 tcttctttta ctaagcctac acggtgaccc atttctgtta atcgcaaatc tgcattatca
    11881 tgacgtaaaa gcagacggta ttcagcacgt gaagtcagca aacgataagg ttcgttcgtt
    11941 ccttttgtta ccaaatcatc gatcatgaca ccgatgtaac cgtcacttcg tttgatcact
    12001 aaaggttctt ttccctgaac tttcaaagct gcattgatac cggcaatcag cccttgtcca
    12061 gccgcttctt cataaccgct tgtaccattt gtttgtccag ctgtataaag attttcaacg
    12121 actttagttt ctaatgtcgg acggagctgg tgtggctcta ctacgtcata ttcgatagca
    12181 tatcctgtac gcatcatttc tactttttct aatccagcaa tagaatgaag catgttgact
    12241 tgaacatcct ctggtaaaga agtagataaa ccttgaacat agacttcttc cgtatttaag
    12301 ccttctggtt ccaaaaataa ttgatgacgt ggtttgtctg caaaacgcac gatcttatct
    12361 tcgatggatg gacaatagcg agcgcctacc ccttcaacga tccctgtaaa cattggggca
    12421 cgatgtaagt tgtctcgaat aatttggtga gtcgtctcac ctgtataggt cagccagcaa
    12481 gattcctgat ctagtttata ggcactatct ggtgtgctga agctaaaatg attcggctct
    12541 ttgtcaccag gctgctcttc tgtaacagaa tagtcgatcg tacttgattt tactcgaggc
    12601 ggtgttcctg ttttgaaccg ttcgatagca aagccaagtt cttttagatg attcgctagt
    12661 ccaatagaag gttgagagtt attcggacct gatgaatatt tcaattcacc gataatgatt
    12721 tctccgcgaa gtgctgttcc ggctgtaata acaactgctt tggcccggta gctcgctcct
    12781 gtggacgtca ctactccatg acaaacacca tcttcgacga tcagttcttc gacgattcct
    12841 tgtcttagcg tcaaatttgg ttctttttcg atcgtatgtt tcatttctgt tgaataagca
    12901 tgtttgtctg cttgtgctcg caatgctcgg acagctgggc cttttcctgt gttcaacata
    12961 cgcatttgga tataggtttt atcgatattc ttacccattt ctccgcctaa cgcatcgatt
    13021 tctcgaacga caacgccttt agctggtccg cctacagaag gattacatgg catgaatgct
    13081 accatgtcca aatttattgt cagtaataat gtttttgatc ccatacgtgc agcagcaagt
    13141 gcagcttctg aaccggcgtg tcctgcgcca acaacgatca cgtcatacgc atctgcttca
    13201 taatgttcca atcgtttgtt cctcctttta ttttcctaaa caaaattgac tgaataattg
    13261 agtgattagc tcatcttgta cactgtcgcc gacaacttct cctagatagt cccagcaacg
    13321 cgtcatatct atttgaacga gatccacagg cattcctgaa tcaatacctg caatgacttc
    13381 ttcaagtgag agggaagctt tttctaacaa cgcgatatgg cgggtattag aaacataagt
    13441 agcgtctctt tctcctgttt ctccgctgaa gaataaatca gaaatagctg attccaaacg
    13501 atctagtccg tcgtttttag ctacggaaat tgaaaaaact ggttcatttt cgatcaattt
    13561 cttcaatttt tcttgttcca gctgtgctgg caaatctgtt ttatttagaa gaatgatccg
    13621 tttcaatcca cttgtcgctt ccaataattg ttcatcttct gctgtcagtg gttcgctttg
    13681 gtttagaaca agtaagatca agtctgcttc tgctaatgct tttcggcttc tttcgacccc
    13741 gatccgttcc accacatctt ctgtttcccg aattccagct gtatcgatta gcttgagcgg
    13801 taccccccga acattgacat actcttcgat tacatctcga gtcgttcctg caatatccgt
    13861 aacgattgct ttttcttctc ttaacaaatg gtttaacaaa cttgatttgc cgacatttgg
    13921 ccggccgatg attgccgtac ttaacccttc ccgcagaacc ttcccttgct tggatgtagc
    13981 aagtagcgct tggattcttt gttggatcat ctgcgctttt tcaagcaata acttcgtagt
    14041 caattcttcg acatcatcat attctggata atcaatattt acttctactt gtgctaatgt
    14101 ctctaaaatc tcttgtctca atgagcggat caaagcagac agattgccgt ctaactgatt
    14161 aagagctagt cccattgctt tgtctgtttt tgcccgaatc aaatccatca cagcttctgc
    14221 ctgtgaaagg tcaacacgac cattcaagaa tgctcgtttt gtgaattcgc ctggctcagc
    14281 taatcttgca ccttctctta acaatagctg caaaatttga ttgactacaa cgatcccacc
    14341 gtgacaattg atttctacta catcttcccg cgtaaatgtc tttggtgctc tcatcacgga
    14401 gaccatgact tcatcgacaa gctgattgct cttgggatca acgatatgtc cataatggat
    14461 cgtatgactt ggtacttctg ataatcgttt gtttccagat tgataaactt tatctgctag
    14521 ttgcacagct tgatcgccac ttaaacgaac gatactgatt gcaccttctc caggtggggt
    14581 agaaattgcc gcaatcgtat caaattctaa agtaatttca gccatttttt cacttccttt
    14641 ttcattttgc gcacaaaaaa agtgcccact ccacccttta tgcacaataa acggtgaact
    14701 tcgcactttg actgcttgtt cttgattaaa ttctggtcat agattatcac acttgctata
    14761 aggaatcaag tgtttaaaac aattagatga agttgagcct gttctgcttc cattacctta
    14821 tatttaattg tccttggatg tctctttgcg ttctttggtt tattcgaaga gaagatactg
    14881 tataaacaag gctttatctt tcttaaaaaa aacggtacac taaatggtag cgctatctaa
    14941 actatacact tttgaaagga gaaaatatgt gagcccgttc agatctgctg cattggaaga
    15001 agaaagtgac cctcggatca acgatgtcgt tttatctaca gggaatatca accagaaata
    15061 tatcgtccgc gatatcgttt ttgttgtgga atgcttagaa gctgatttgt ttgacccatc
    15121 ttttcccaaa gatgaccttt tcttaacaac gaagaggaaa ttaaagacga aggtacttga
    15181 atatggtgcg aatgcagtaa tcaactgtca ttttgagtat cagcaaaaaa aacgcgacgg
    15241 aaagtatttt ctggaacttt ttgcttatgg aacagtgatc caatttatcc aaacaacgat
    15301 tggttaaatg aaacagacaa aaaaagcgga tcaaaactga agacagtttc gatccgcttt
    15361 tttctatcaa agttagtgac tacttcatcg tatactctga taagaatgat ttctagtaaa
    15421 atatacttgg tttgaaaaac tttgtcaacg tgatttattc aaaaagtaat ggaatcaata
    15481 atatttttta gcaggttcta ctactaaata acgataaggt tcctcacctt ccgaatgtgt
    15541 ttttacatgt tcatctttgc ttaatgcagc atggatttgt ttacgttcaa atgcaggcat
    15601 tggttctaag aaaacaggct gccctgtccg ttttactttt tcagccgtgc gatcagctaa
    15661 acgctgaatg atctcttggc gcttttcacg gtaattgcct acattgacta ctacagacag
    15721 cttatttctt gctaaacgat ggatgaagac ttgtgccaga tattgcaatg cattcagtgt
    15781 ttttccatgt ttcccaatca gtatgccttg cttttgtgtt tctaaatgga aaacaaccat
    15841 attatttgat tctctttcca ttttgacaag agctggagca tcaagctgtt ttgaaatttc
    15901 agtaagataa actgccaatt ccgttaaagc tgcttcatca cctagctcac ttacttcttc
    15961 tgttttttct tcaagaatcg tttcgttgac tacctcgatc tcagctgttg gttcttcttc
    16021 caaaatttct tctacagtct gttctactgt ttcagaaact ttttcgctga tacttggctg
    16081 gatcgaaagc cgtgcattct ttcgacccat tcctaggaaa cctttttttc cttcttccag
    16141 tatttcgatt tcgacttggt ctttggccaa acccaaatca gccagaccta gttggacagc
    16201 ttcgtctatt gttgcagctt cataaatcgg cattagcatt acctccccta ttatttccgt
    16261 tttttcttag gattcattgc tttcttaaga gcacgttctc tttcgcggat tctccgtgct
    16321 tcttcttcac gctcttttcg aattttgaaa gggttgttga tcagcagcgt ttgtacaact
    16381 tggaaggcat tggaaactac ccagtataat gaaagaccgc tggctaaatt gacacccatc
    16441 actaaaatca ttacaggcat cacgaaattc atgatcttca ttgatgcatt cgattcaatt
    16501 tgactcatgc tagataaata cgtacttgca aaagtaaaga ttgctgctaa aatcggcaaa
    16561 ataaatgttg gatccgcgtt ccctaagttc aaccataaga aatgcccttg tttcaactct
    16621 ggtacacgtg aaatagattg ccataacgcc atcaagattg gcatttgtac taataaagga
    16681 agacaaccag catatggatt gacgccattt tctgcatata aacgctgctg ctcttctctt
    16741 aacttacttt gtgtttccgg atctcttgaa ctatattgtt gctgcagtgc ttttaatttc
    16801 ggttgtaatt cttgcgtttt acgcatgctc tttgtttgga aatgcatcaa cggcaatagg
    16861 atcacgcgga tgatcagtgt aaataaaatg atccctatcc ctgcattacc aaatgacaac
    16921 gccttgatcg cctcagcaaa gtaataaaca atataacgat cccaaattcc tgtactctgt
    16981 gcgctgactt ccccagttcc gcaaccggat aagacgatga ccaaactaac gataccggcc
    17041 atcaaaagta aacgtttgta tttcttcact tactcttttc ctctctgtcg attatttttg
    17101 caagttttaa gacgtggact aaattggagc gaacctcatc ataagtcaac cccttcacac
    17161 ttggtctagc aatcacaata aaatccaact ccggatcaat ttcttctttt atagaataaa
    17221 ctgctgctcg gatttttcgt ttgacttcat tgcggtttac cgcgttgccg accttttttc
    17281 ctacagataa accaactcga aaatgaggct gtcccggttt ttccaaacgg taaacgacga
    17341 atttgcggtt agcaaaagaa tctcccgtat ggaacacggc ttgaaactct cgttcttttt
    17401 ttactcgata cgcttttctc attagttttc tttccttttc tatctctcac ttacgctcat
    17461 aatcatacca taattacgat tgtcctttca tcatttccct aaaaaagagt ataaaaaaaa
    17521 ccactgaaac cccagtggcc taagctgaaa gaacttttct tcctttgcga cgacggctag
    17581 ctaacacacg acgtccattt ttagtactca tacgtttacg gaatccgtga actttttgac
    17641 gtttacgttt atttggttgg tatgttcttt tcatatattc cacctccaaa atgttcttat
    17701 ctattttata aacacagaca tactttgata gtataacgac aaagtagtga ttttgtcaat
    17761 ataaaataga agatcgattg ttaaaactta tccacaagat tatttttttc tatgggaatc
    17821 ctatgtggaa aaagttatgc acagggatta tccacagata ataaaaggct ggttttacac
    17881 agaaaacaca cgtgtaaaaa aagtaatcca caatcaggcg atttcctgtg tattccgtct
    17941 cctaactatt ggtatatctg tattttttat ccacagacgc aagaggaaat ctattggttt
    18001 ttacgagttt tccacctgtg cattagtggt gtggaaaact ttttttgaca actgtgtttt
    18061 tctctttttt ctttttgtcg attgtgtgga taattgtaat taaaaaattt ttttcttcgg
    18121 tttctgtgga aaactattga aaaaaatgct aaattagtac tagttattca ctttttaatg
    18181 aaggaggagg ccttatatgg tatccctcga tgctttatgg aatgaattaa aagcaacata
    18241 ccaaaaggat ttatcgcctg ctagctataa tacatggatc gaaacagcga accctcgaac
    18301 ccttgatcaa aaccagctcg ttgttgaggt tcctagcaag attcataaag aatattggga
    18361 aaaaaacctg gcgacaaaaa tcgttgaagt cggttatatg ttatcaggaa atgagattat
    18421 ccctcgcttt attactggcg aagaagcaga gcaagaagaa gttatagaag aaaaaaatcc
    18481 aaaagttgtg gcacctagtc cgctaaaaaa agccatgctg aaccctaaat atacctttga
    18541 tacgtttgtc attggtaaag gaaaccagat ggctcatgca gcagcgctcg ttgtagcaga
    18601 agatccaggt tctatttata acccgctctt cttttatgga ggcgtgggac ttggtaagac
    18661 tcacttgatg catgcaattg gtcatcaaat gctgcaaagt cagcctaatg ccaaagtaaa
    18721 atatgtaagc agtgaaactt ttgctaatga tttcatcaat tcgatccaaa acaaaacagc
    18781 agaagagttc cgtcaagaat atcgaaatgt tgatttactg ctagtggatg acattcaatt
    18841 ttttgcagaa aaagaagcta cgcaagaaga gtttttccat actttcaatg ccttgtacaa
    18901 tgaagggaaa caaatcgtac taaccagtga tcggttgcca aacgagattc cgaaactgca
    18961 agaacgcctt gtttctcgct ttgcatgggg gctgtctgtt gatattacac cgccggatct
    19021 tgaaacaaga acagcgattt tacgtaaaaa agcggcggct gaacgtctag agatcccaga
    19081 tgacacacta agctacatcg caggacaaat tgattctaac attcgtgaat tagaaggtgc
    19141 gcttgttcga gtgcaagctt ttgctgcgat gaatagtgaa gatatttcga ctagtctagc
    19201 tgcagacgca ctgaaaacgt taaaatcagg aaaaggacat cctcaattgt caatcctgca
    19261 gatccaagaa gaagtggcaa agtattatca catccaattg aaagatctca aaggaaagaa
    19321 acgcgtcaaa tctattgttg ttcctagaca aatcgctatg tatttggcac gtgagctcac
    19381 agataattct ttaccgaaaa tcggagctga attcggtggc aaggatcata caacggttat
    19441 ccatgctcat gaaaaaatcc agcagctgat ggattctagt gtcagtatgc aaaatgaagt
    19501 atcagaaatc aaaaactttt tactaaatta atttgtcgat tctatcaact gaaaatagca
    19561 tgtggataaa agaaaaaaac gttcaaaagt tatacacact ttttccacag ctgttttttc
    19621 gtttctctct tacgtttttt gagttttcca cagaatcaac atgccctatt acttttatta
    19681 ttctttttaa ctaataataa ataaacaaac attcaaagga gatacatcat gaaagttact
    19741 ttaaaccgag ctagctttat gcaggaattg caaactgttc aacgagctat ttcaagcaaa
    19801 accacgatcc ctattttgac aggtgtaaaa atcacactga cacaagaagg tttgactttg
    19861 acggggagca acgctgatat atcaattgaa acttttttgt ctgttgaaaa cgaaaaagca
    19921 aatatgcaaa tcgaatctac tggttccatt gttttacaag cacgtttctt tagcgaaatc
    19981 attcggagac ttcctgaaga aacatttact ttagaagttt tagaaaataa acaagtagcg
    20041 atcacttctg gaaaagcgaa ttttatcgta aatggattag atgcagataa ctatcctcat
    20101 cttcctgttg tcgaaagcca taaccagatg aaattacctg tacacgtatt gactaaacta
    20161 atcaacgaaa cagtttttgc tgtctcacaa catgagagtc gtccaatctt gacaggtgtg
    20221 catttcattt tatctgataa ttctttatta gctgtagcta ctgattctca ccgtctaagt
    20281 caacgcgtga ttccagtaga acaagcggct gatcattttg atattgttat tcctggaaaa
    20341 agtttgatcg aattatctcg ctcattaaca aatgaagaag aaatcgtcga aatcagcatt
    20401 atggaaaacc aagtgttgtt caaaacagaa acgatgtatt tctattcccg tttgctagaa
    20461 ggaaactatc cagataccaa tcgtttgatt ccttcaagtt ttaatacaga ggttgaattt
    20521 tctgttccta gctttttagc ggcgatcgaa cgggcttctt tactttctca tgaaggccgt
    20581 aacaacatcg ttcgtttatc tattcgtcca gatgcggttg ttttatacgg aaattcgcca
    20641 gaaatcggga aagtagaaga aagcttgagt tatacagcaa gcagtggcga tccattagat
    20701 atttctttca atccggatta tatgaaagca gcgttgcgtg catttggaga tatgagcatc
    20761 aaagtgaaat ttatctctgc tattcgtcca tttacattgg aaccaacgga agatggcgtt
    20821 caatttattc agctgattac tcctgtacgt acaaattaac aatgattttc aatgttcttg
    20881 aaaatttaat tgaaaagaaa tagaaaaaat aaaattttaa acagaaggcc ctaaaatctc
    20941 ttttaaggct ttctgtttat ttttgtttaa atcacctttt ttagattaaa tcgtcttata
    21001 tggaaaaata tatggataaa tttgttttat tgcgaaaaaa aaggtataat agagtatact
    21061 tttaataatg aaaatgaggg atgggcattg aaacaacaag tcattctaaa tacggaattc
    21121 atgacgttgg gccaaatgtt gaaagaagtc acagttatcg gtagtggcgg acaagcaaaa
    21181 tggtatttgg cagaaaacac tgtgttagtc gatggcgaac cagaaaatcg tcgtggacgc
    21241 aaactttatc ccggcatgat ggttgaagtt ccagaagtag gaactttttt tatggtaaaa
    21301 aaaggaaacg acgataatga ggctgaataa gctgtattta aaaaattatc gaaattacga
    21361 agagctgaac atcgaatttt ccaagaacct cgtcatcttt ttaggagaaa atgcgcaagg
    21421 aaaaacgaat cttttggaaa gtatctacgt attagctatg acgcgaagtc atcggacaag
    21481 tagtgagcag gaattgattg gctggactga cgatcaggcg atgattcaag gagagatcac
    21541 aaaaggttca tccactcttc cattagaaat cttattatct aaaaaaggcc gaaaaaccaa
    21601 gatcaaccat atcgaacaga aaaagttaag tacttacgtg ggacaaatga atgtcatttt
    21661 atttgcacca gaagatcttt ctttagtaaa aggcagcccg cagatcaggc ggaagttttt
    21721 agatatggaa ttaggccaga tcaatccaat ctatctttat gatcttgtcc aataccaagc
    21781 aattttgaaa caaagaaacc agtacttgaa gcaattgaat gaaaaaaaac aaactgattt
    21841 gctttatttg gatattttgt ccgaacaact agcagcgttt ggcagcaaag tattgaaagc
    21901 aagagcacag ttcgtcaagc gattggagtt ttgggctaat tctttgcatc agcaaatcac
    21961 ccatcaaaaa gagcagctgg aaatcgagta tctcaccgca gtcgattctt tagaaacaca
    22021 tacgcaagag caaatccaag aacaatttct cgcacttttg aaccaaaata aaaagaaaga
    22081 cctttttcgg ggaacaacga cagttggtcc tcatcgtgac gatctttcct tttttattaa
    22141 tcagaaaaat gtgcagactt atggatcgca aggacagcaa agaacaactg cactaagtgt
    22201 caaattagca gaaatcgatt tgattaaaga agagacaggg gaatatccaa tccttttgtt
    22261 agatgatgtg atgagcgagc tggatgataa tcgtcaactc catctgctag aaacaatcga
    22321 aggaaaagtt caaacgttct taacaacaac gacacttgac catgtaaaaa ataaaatgac
    22381 tgttgaacct gagatttttt atgttcaaca agggaaacta aaaggagaag aaactgaatg
    22441 acagaagaaa gaagtttagt agaacgcgct aaagagtatg atgccagtca gattcaagta
    22501 cttgaaggac ttgaagccgt tcgtaaacga cctggcatgt atatcggttc gactagttct
    22561 gagggtctgc atcaccttgt ttgggaaatt gtcgataatt cgatcgatga agtattagca
    22621 ggttttgcaa cgaaaatcca tgtgattatt gaaaaagaca acagtatcac cgttattgac
    22681 gacggtcggg ggatacctgt agatatccag gcaaaaactg gacgtcctgc agttgaaacg
    22741 gtctttactg ttttacatgc tggaggaaaa ttcggcggtg gtgggtacaa agtttctggt
    22801 ggacttcacg gagtaggatc ttctgttgta aacgcgcttt ctacacagct tgatgtcaaa
    22861 gtatacaaag aaggaaatgt gtactatcaa gaatatcgtc gtggagctgt agtggacgac
    22921 ttaaagatta tcgaacaaac ggatcgccat ggaacaacgg ttcactttac gccagaccct
    22981 gaaattttta cagagacaac tgagtttgat ttttcaaaat tggctacccg gatccgtgaa
    23041 cttgctttct tgaaccgcgg gatgcgtata tcgattgaag acaaacgaga agaagaacca
    23101 gtcatcaatg aataccatta tgacggcggg atcaaaagtt atgtcgaata cttgaatgca
    23161 aacaaaaccg tgatttttcc agaacctgta tatttggaag gtgaacagca agatatcgca
    23221 gttgaagtct ctatgcaata tacagacggc taccattcca atatcatgag ttttgccaac
    23281 aatatccata cgtatgaagg tggaacacat gaatcaggat tcaagactgc tttaactcgt
    23341 gtgataaatg attatgcccg aaaacaaaag ctgatgaaag aaaatgacga caacttgacc
    23401 ggtgaagacg tgcgagaagg attgacagct gttatttcta tcaagcatcc tgaccctcaa
    23461 ttcgaaggac aaacaaaaac aaaattgggc aattccgaag tacggacagt cactgatcgg
    23521 ttatttagcg aacatttcat gaaattcttg ctggaaaacc caagtgtggg ccggcaaatt
    23581 gttgaaaaag gattactagc ttccagagct cgtctagcag cgaaacgtgc gcgtgaagta
    23641 acgagacgta aaggtgcttt agagattagt aatctgccag gaaaattagc ggactgctct
    23701 agcaacgatc ctgaaaaatg cgaattgttc atcgtcgaag gagattcggc cggcggatca
    23761 gctaagcaag gtcgtaatcg agagtttcaa gccatcttgc ctattcgtgg gaaaatcttg
    23821 aatgttgaaa aagcaagcat ggacaagatc ttggcaaatg aagagatccg gtctttattt
    23881 actgctatgg gtacagggtt tggagaagat tttgatgtat cgaaagctcg ctaccacaaa
    23941 ttagttatca tgactgatgc cgatgtcgac ggcgcgcata tccgtacatt gctgctgact
    24001 cttttttatc gttacatgcg tcctattgtt gaagctggat acgtctatat tgctcagcca
    24061 cctttatacg gggtaaaaca agggaagaat attacgtatg tacaaccagg aaaaaatgca
    24121 gaagaagagt tgaaacaagt cgttgcttca cttcctgcct caccaaaacc aagcgttcag
    24181 cgttataaag gacttggaga aatggatgat catcaactat gggaaacaac tatggaccca
    24241 agcaatcgga tgatggcccg cgtcagtgtg gacgatgcta ttgcagcaga tcagatattt
    24301 gaaatgttga tgggggatcg agtagaaccc cgtcgtgcat tcatcgagga aaatgcacac
    24361 tatgtgaaga acttagatat ttaatcatga aggaggaaaa agaatgagtg aagaaatcag
    24421 agaaaacatc catgatgtca atctaaccag cgaaatgaaa gactccttca tcgattatgc
    24481 gatgagtgtt attgtcgcac gtgcacttcc tgatgtcaga gatggtctga aacccgttca
    24541 tcgccgtatt ttatacggca tgaacgaatt aggtgtaaca ccggataaag cccataaaaa
    24601 atctgccaga atcgtcggag atgtcatggg taaataccat cctcatggag acattgcgat
    24661 ctatgagtca atggtacgga tggcacagcc attcagctac cgttatatgc tagtggatgg
    24721 tcatggaaac tttggttctg ttgacggtga cggtgccgcc gctatgcgtt ataccgaagc
    24781 acggatgagc aaaatcgcta cagaaatgct gcgtgatatc aataagaaca ctgttgattt
    24841 ccaaagcaac tatgatgata cagaaaaaga accagttgta ctacctgctc gcttcccgaa
    24901 tcttttagtg aacggaacaa cggggatcgc cgtaggtatg gccacgaata tcccacctca
    24961 taacttgtct gaggtagtag cggctatcga tttgctgatg gataatcctg atgtgacgac
    25021 caatgaattg atggaagtat tacctggacc agattttcca acaggtggtt tggttatggg
    25081 gaaatcagga atccgaagag cgtatgaaac aggtaagggt tctattaccg tacgtgcaaa
    25141 agttgagatt actgagatgc caaatggtaa agaacggatc atcgtgacag aattgcctta
    25201 tatggtcaat aaagcaaaat tgatcgaacg gatttctgaa ctgcacagag acaagcgtat
    25261 cgaagggatc acagatttgc gggacgaatc ttcccgtgaa ggtatgcgga tcgtcattga
    25321 catccgtcgt gacgccagcg catccgttat cttgaataac ttatataaat tgacatcctt
    25381 gcaaaattca tttggattca atatgttggc gatcgaaaaa ggtgtgccga aagttttgag
    25441 cttgaaacaa atccttgaaa actatatcga acatcaacgc gaagtcatca cacgacggac
    25501 ggtctttgaa aaagaaaaag cagaagcaag agcacacatc ttagaaggtt tgcgtattgc
    25561 tttggatcat atcgatgaaa tcattgcgat tatccgcggg tctaaatcag atgacgaggc
    25621 aaaagcaaca atgatcgaac ggttcgattt atccgatcgc cagtctcaag ctattttaga
    25681 tatgcgttta cgccgattga ccggtttgga acgggaaaaa atcgaaaatg aataccaaga
    25741 actcttaaaa ctgattgaag atttaactga cattctggca agaccagaac gtgttacaga
    25801 aatcatcaag aatgaattag cagacttgaa tcaacgattt ggtgataaac gacgcacaga
    25861 attactagtc ggtgaggtct taagtctgga agatgaagat ttaatcgaag aagaagaaat
    25921 cgttattaca ttgacaaaca atggttacat caaacgtttg gcaaataatg aattcagagc
    25981 tcaacgaagg ggtggacgtg gtgtccaagg aatgggtatc cacaatgacg acttcgtcaa
    26041 aaatctggtt tcttgttcta cccatgatac attgttattc tttacgaata acggaaaagt
    26101 gtatcgggca aaaggatacg aaattccaga atacgggcgt acagccaaag gaatcccaat
    26161 catcaattta ctaggtatcg attcgagtga aagcatccag gcaatcattg cggtgacggg
    26221 agaagcagaa gaaggccact atctcttctt tacgacgcgt caaggtacag taaaacgtac
    26281 ttctgtcact gcttttgcta acatccgaag taatggtctg atcgcaattg gtttgaaaga
    26341 agacgatgag ctcgtcaatg tgctactgac agatggtcaa tctaatatca ttattggaac
    26401 acataacggc tactctgtca ctttcaaaga agaagcagtc cgcgatatgg gacgtacagc
    26461 ttccggggtc agagggatac gcctgcgtga ggaagattat gtcgttgggg cagccttaat
    26521 gaaggaaaat caagaagtat tagtgatcac agaaaaaggt tacggaaaac ggacaaaagt
    26581 atcggaatat ccagtgaaag gccgcggagg taaagggatc aaaacggcga acatcactga
    26641 aaaaaatggt ccactagctg gattgaccac tgtttctgga gatgaagaca tcatgctgat
    26701 tacagataaa ggtgtcatta tccgtttcaa cgtatcgact gtttctcaaa ctggacgttc
    26761 aacattaggc gtacgtttga tgaagatgga agctgatacg aaagttgtca cgatggcagc
    26821 agtcgaacca gaagctgcag aacaaacagt tgaagaacaa ggatcagaag aataaccatc
    26881 aagactaagt gtgtaagcta gcaagaataa ccgattaaaa aatctggtta ttcctgctgg
    26941 cttttttgat tagttgaatt tctcttaaaa tattcgaaac tgttcattct gtattgcttt
    27001 tgaccaaaat agagtgtata attagagctt gtgagtagtc taattaatta gataactctc
    27061 cttgctctta ggaacctaag agccgaaagt ccacagggag gtgaatatca atggaaaaca
    27121 cgaaatatga aatcatgtac attattcgtc caaacattga tgaagaagca aaaactgctt
    27181 taatcgaacg tttcgatgca atcttgaaag ataacggagc agaagttatc gaatcaaaag
    27241 attgggaaaa acgccgtttc gcttatgaaa tgaacggtta ccgtgaaggt atctatcaca
    27301 tcgttaaagt atcttctcca tcatcagcaa atgctgtcaa tgaatttgac cgtcttgcta
    27361 aaatcaacga cgatatcatt cgtcacatga ttgttaaaga agaagcttaa ttgttgtttc
    27421 atgtgaaaca atagaaaacg atgagaggag atgactattt tgattaataa tgttgtatta
    27481 gttggacgtc tgacgaaaga tccagatttg cgttacacag caagtggtac ggcagtcgca
    27541 acatttactt tggctgtgaa tcgtaatttt acaaaccaaa gcggcaatcg cgaggctgat
    27601 tttatcaact gtgtgatttg gcgcaaatca gcagaaactt tagcaaacta tgctcgtaaa
    27661 ggaacacttt taggtgttac tggacgtatc caaactcgtt cttatgataa tcagcaagga
    27721 caacgtgtat acgtgacaga agtagtagct gacaacttcc aattgctgga aagtcgttct
    27781 gcatctgaaa atcgccaaca aagcggcgga ttccaaagtt ctggtcaatc agctggcgga
    27841 tttggcggaa acaataactc taatcaaaca tctcaatcat ccaacggtat gcctgatttc
    27901 gaccgtgata cttctgaccc attcggctca agttcaacga tcgacatttc agacgatgat
    27961 ttaccattct aaacgaattt gataggaggg aaatagaatg gcacaacaaa gaagaggcgg
    28021 acgtaaacgt cgtaaagtag actacattgc tgctaaccat attgaatata tcgattataa
    28081 agatatcgaa ttactaaaaa gatttatttc agaacgcggt aaaatcttgc cacgtcgtgt
    28141 aactggtaca ggcgctaaaa accaacgtaa aattactgtt gcaatcaaac gcgctcgtat
    28201 catgggcttg ctacctttcg ttagcgaaga ctaataaatc ctaggaattg aatgactgat
    28261 ccttttgatc agctattcaa ttcctttttt tctgtaaaaa taacctctat tcgtttagtc
    28321 gctcgaatga cgaaagttat ggtaaaatca aattagtcat atatgggagg aaaattatgg
    28381 aaaataaaaa caatcattta tccccgaaac ctctcttcct tatcctgttt atattaatcg
    28441 aagtattggt agtcttaatt ttaccatttc gttttgttag cgcttcggtc attctattga
    28501 tgaatttatg gggtgttcgc tattggttga aaatgaaacg ttggcttatt ttaaataaac
    28561 gtgaaaaaat ccagcaggct tcgactgtag ctgaaaccaa tctagcttat gtctcagaag
    28621 tcatgcctgt aggagtgatt gcttatcagc catcaagcca aaaaatcgaa tggctcaatc
    28681 cattagcatt gaaaatcaaa gatcaaagtg gattaacaga ggaagaattg ctagatactt
    28741 tttttactgc aaaagaaaaa aagaaaaacc aagtctatct agcagataaa acatttcagt
    28801 ttgatatgga tacagtaaag ggtgttgttt attttaatga tattacccaa aaccagttgt
    28861 taaaactcca tcagacgaac agtcagccgg tgattggtat tttatctatt gataactatg
    28921 cagatgccat tgataaatta gatgacaaag agatttctta tttgaactct ttcgtcacta
    28981 cactagtttc tgactggatg aatgaatacc atgtcttttt caaaagaatc aacgcagaac
    29041 ggtttttctt cgtagcacag atgatcgatc tgaaaaagat gatggataaa aaatttgatt
    29101 tgctggatcg ctttcgagca gaagctgaaa aacagagctc gcctatcacc cttagtatgg
    29161 ggattgctta tggctcaaga accctcgatg aaatcggtgg agaagctcag aataatttag
    29221 atgttgcttt agtccgtgga ggcgaccaag ttgtcgtgaa agaggtaaaa gaaggagcaa
    29281 aacctgttta ttatgggggg aaatctgcta agaatgtcaa acgcacgcgt gttcgttctc
    29341 gagcgatgag taccgctttg cgtcggattt ttgatgaaac aggcgatgtg tttatcatgg
    29401 ggcacaagtt tccggatatg gatgctttag gctctgcttt tggtgtcagt tgtctggcac
    29461 gcttcaacca gaaaaaagct tacatcgtta tcaatgagaa cgagatcatt ccagatgtag
    29521 aacggtgtct tgaagaaatc cataagcatc ctgaattaga aatgcaacta atctctccag
    29581 aacgtgccat agagctgaaa aaagaagagg acctcctcgt gatggtggat taccataaac
    29641 cttctctatc gatctctcag ggcctatatg agcaatttga taagattgtg atcattgatc
    29701 accatcgtcg tggggaagag tttccaagta aacctctgct gacttatatt gaatcttcag
    29761 cttcttcagc ttcagagcta gtttctgagc tgatccaata ccaaagcagc aaaacaaatc
    29821 gcttagatcg ttttgaggcc acattattgc ttgcagggat ggttgtagat acaaatagtt
    29881 tcaatgtccg tacaactgca cgaacttttg acgttgccag ctatttaagg tcttgtggag
    29941 cagacgcatc cttagtacaa tatttattga gctcggattt agcgacatac ttggagatca
    30001 gcaatttgat tgctggaagc gactatgtga cttctgatat tgttgtggcg gcagggacag
    30061 acgaaaaaga gtataatagt gttatagcag caaaaacagc agatacattg ctttcgatga
    30121 tcaatatcaa tgcagctttc gtcattacaa agcggacaga cggcttgatt gggatcagtg
    30181 ccagaagtac cggcacgatc aacgtccaaa tcatcatgga atccttaggt ggcggaggac
    30241 acttcacaaa cgcagctact caactggaaa atacgacagt tgaggaaaca aaacagaagc
    30301 tatttgctgc aatcaaagaa aatatgaatc aaatatatga taaggagtaa tccaaatgaa
    30361 agtaattttt ttacaagatg taaaaggaaa aggtaaaaaa ggggacatca aagaagttcc
    30421 agcaggttat gcacaaaatt atctaatcaa aaataacttg gcaaaagaag caacgaaaag
    30481 cagtatcagt cagttagcag gacaaaagaa agcacaagaa aaacacgaag ctgaaatctt
    30541 ggaagaagct aaaaagttaa aagaattctt ggaaaaagaa gatactgtag tcgaattgaa
    30601 ggcgaaagca ggggaagaca gccgtttgtt tggttcgatc ccttcaaaac aaattgctga
    30661 agggttaaac aaacaataca aaatcaaatt agataaacga aaaatcgaat tagaaaatcc
    30721 gatccgttca ctaggctaca caaacgtacc tgttaagctt catcatgagg tgacggctac
    30781 gatcaaagtt catgtcgtac aagaataaca aatcttgata gcgatgttca gctggtaagt
    30841 ataaccagaa ggaaaaaaga caaaccgaaa tttatctgag aaaacagctg cagcaatgaa
    30901 agcaaaagct gcaaatcgat ttcagattga tttcggtttc ttttttggga acagcaaaat
    30961 agcataaaga aaacgagtat aataaaagag aactattttt ttattacact ttcggtacga
    31021 atgatgactt ttctctcatt ttcttgtaaa atgaagcaat tgaagacaag gagcgatacg
    31081 tatgaatgaa gtgtggcagg atcgaatccc accgcaaaat attgaagctg aacaagcggt
    31141 tttaggctct gtttttcttg acgcagaaac gatcattgat gccatggagt acatagagcc
    31201 aaaagacttt tatcgtcgtg gccatcaaat tatttttgag acaatgatcg agttaaatga
    31261 tcgtaatgaa gcgattgacg tggttactgt caaagaccgg ctggaacaag ccaatttgtt
    31321 agaagatgcc ggtggtttaa gttatctctc agacttggca ttagcagttc cgacagcagc
    31381 aaatatcgtc tactatgcga aaatcgttga agaaaaatca ttgctgcgta cattgatcca
    31441 gacagcaaca ggaatcgtta cgaaaggatt cgagcaagga gaagaagttc aaacaatttt
    31501 agatgatgcc gaacgcagta tcttggaggt ttcggaaaaa agaaatcgaa gcggcttttt
    31561 atctattgct gaagtactga actccacgat tgaaaatatc gatcgactag ctcaaaacaa
    31621 tgaagaaatc acaggtctac caactggcta tcaaggtctg gataaaatga cagcaggttt
    31681 ccagcccgaa gaactaatca ttcttgctgc acgtccagcg gtaggtaaaa cagcgtttgc
    31741 attgaatatt gctcaaaatg taggaacaaa aacagatcgt tctgttgcga tatttagttt
    31801 ggaaatgggg gcagaatctc ttgtaaatcg gatgctttgt gcagaaggtt cgattgaggc
    31861 cagccattta cgtacaggtc agctttcgga agaagaatgg tcgaatctga tcgttgcgat
    31921 ggggagtctt tctcgagcaa atatttttat cgatgataca ccagggatca aaatctctga
    31981 aatccgtgcg aaatgtcgga aactagcgca agaaaaaggc aatctaggct tgatattgat
    32041 cgattatttg cagttgattg aaggtaacgg taaagagaac cgtcaacaag aagtatcaga
    32101 tatctctcgc caactgaaaa aactggcgaa agagctgaaa gtacctgtca ttgctctttc
    32161 acagttatct cgtggagtag aacagcgaca agacaaacga ccagtgctaa gtgatattcg
    32221 tgaatccgga tcaattgaac aagatgcgga catcgtagcc ttcttgtatc gagatgatta
    32281 ttatgaaaga gaaggcggag atgacgatga tggaaacgag ccaaaaaatg acaacgtcat
    32341 cgaagtcatc atcgagaaga accgaagcgg tgcacgagga acagttgaac tactgtttat
    32401 caaagaatac aataagtttt cgtcgctttc gccaagagaa gaattttaaa gaagaggttg
    32461 tgagccttga gcaatcccga gagctctcgt aagaagatgc aaggagtaac cgcaggaacg
    32521 actagagtac ttaatttaaa cagatctcca aagggattgt gacaaaagtc ttgtcacagt
    32581 ccctttttcc gaataaacgg tgttcaatca gctgaccctt ggtattagct cagcaaacgt
    32641 caaaatatga ggaactattt tgacgtttgt ttctctaata ctcaggtcaa aacgctgatc
    32701 ttacgacctc ttttccttaa acggtgttct cctgccaact cctcggtcac aagtcacaat
    32761 atcatcaaac atgagaagca gttttttgaa attgctcctt gtgactcaga gttaaacggc
    32821 aggctcacaa cctctttctt aaacggtgtt caaaagcttg catctgcggt aatacgccca
    32881 atcaaacgaa aaatatgaga agctattttt gtttggctgt tcttattact tgatgcagga
    32941 cagctttttc acaacctctg attcacggtg ttccaaaagt agcttcttcg aaaaataagt
    33001 cgaaatttca caaaaatttg agaagcaatt ttcgtacatt ccttcttatt tcttgaagct
    33061 gaccacttct gttacaacct cttttttaat aaccattcta aaaagataaa gttcgttttt
    33121 taagcgtttt tataaaataa aaataaaaat cattcgattt tatttgaaat atgtatctat
    33181 tattgataca atggttcagg attaaataat atagaacgag gtgttcacat gtcatctgta
    33241 gtagtagtag gtacgcaatg gggcgatgaa gggaaaggaa aaatcacaga ctttctcagt
    33301 gaaaatgctg aagtgattgc acggtaccaa ggtggcgaca atgcagggca cacaattaag
    33361 tttaacggtg tgacttataa actgcatttg attccttctg ggatttttta taaagacaag
    33421 atcagtgtga tcggaaacgg cgttgtagtc aatcctaaat cattggttaa agaattagct
    33481 tatttacatg aaaaaggtgt agaaacaagt aatttgcgta tttctgaccg cgcgcacgta
    33541 atcttgcctt atcatatcaa attggaccaa ttacaagaag atgctaaggg cgaaaataag
    33601 attggtacaa cgattaaagg aatcggacct gcctatatgg ataaagcagc tcgtgtaggt
    33661 atccgtattg cagacttatt agataaagaa atttttgaag aacgtcttcg tttgaatcta
    33721 gatgacaaaa accgtcaatt tgtaaaaatg tttgattcag aaccacttga ttttgacgat
    33781 atttttgaag aatattatga atacggacaa caaatcaaac aatacgtcac agatacttcc
    33841 gttatcttga atgatgcttt ggatgcaggc aaacgcgtgt tgtttgaagg cgcacaaggt
    33901 gtaatgctgg atatcgacca gggaacttat ccttttgtta cttcttctaa cccagtagcc
    33961 ggtggggtaa caatcggaag cggtgtaggg ccttcaaaaa tcaataaagt tgtcggcgtc
    34021 tgcaaagcct atacgtcacg tgtcggtgat ggaccatttc caactgaatt atttgatgaa
    34081 acaggagaac aaatccgcga agtaggacga gaatacggta cgacaactgg tcgtccgcgt
    34141 cgtgtaggct ggtttgattc tgtagttatg cgccattcta aacgagtttc aggtattacc
    34201 aacctatctt taaactcgat cgatgtatta agtggattgg aaacagtaaa aatttgtacg
    34261 gcttatgaat tagatggcga actaatctac cactatccag caagcttgaa agagttgaac
    34321 cgctgcaaac caatctatga agaattgcca ggctggtcag aagacattac aggatgcaaa
    34381 acacttgcag aactgccaga aaacgcacgt aactatgtac gtcgtatctc agagcttgtt
    34441 ggtgtacgta tctcaacatt ttctgtcgga cctgatagaa cacaaacaaa catcttagaa
    34501 agcgtctggg cgcaaatcta aggataaaaa cgacgactga atcaggacgt tcttaccaga
    34561 gcgagtcgaa gtgtttatct atacgataat aataacttga agaggtagtg aaagagacgt
    34621 ttagccttat tgccgatagg ctgtctcttg aacactgttt attcagaata agggactgtg
    34681 acaaaaaccg tgtcacagtc cctttttccg aacaaacggt gttcaaaagc ttgcatctac
    34741 ggcactaagc ccaatcagat gaaaaatatg gagtgctatt tttatctgat tgttcttact
    34801 gcttgatgca gagcagcttt ttcacaacct ctccactaaa cggtgttctc ctgccaactc
    34861 ctcggtcaca agtcacaata ttcaacaaac atgagaagct gttttttgaa attgttcctt
    34921 gtgactcgga gctaaacggc aggctcacaa cctcttggta aacggtgttc aatcagctga
    34981 ccctcggtat tagctcaaaa aacggcaaaa tatgaggaac tattttttcc gtttttttct
    35041 ctaatactca ggtcaaaacg ctgatttcac aacctctgat tcacggtgtt ccagaagtag
    35101 cttctacgga aataagccga aatttcacaa aaatttgaga agcaattttc gtaaattcct
    35161 tcttatttct tgaagctgac cacttctgtc acaacctctt attttgatta gaacttgcga
    35221 aaataaaccg gttaaagtgt gtctagcgca agagatttgc tataatagac gataagggta
    35281 aaatgtaacg agaaggggtt gaaaaatgtg tatcaaattg taacagattc ttgttgtgat
    35341 ctgccatacc atgtattagc aaaagaaaaa gttgattttt tatcaatgaa tctccaattg
    35401 aatgatcaag agttagtaga tgacctcgga aagacattca attatgaccg ctacatgaat
    35461 cagctgaaaa atggtgcgat gcctacgaca tcacaaatca atgttggccg atatttagaa
    35521 tttttccgtt cttatgcgga aaaacagatt cctgttttat atgtggcttt ttccagtgga
    35581 ttaagcggct cttacagtag tgcgcttcaa gcggttgaat tgctgaagga agaatatgaa
    35641 gaccctgaga tctatgtttt tgatacaaaa gctgcaagta ttggggaagg gcttttagta
    35701 ttggaagctg taagattgaa aaaagaaggt aaaagcatcg aagaaaccct agagtggctg
    35761 gaaagtaatt atttgaaagt ccattcatgg gtaacagtag acgatctgaa atatctagaa
    35821 cgaggcggta ggatttccaa agcaagtgct actcttggca gtctattgag tgtaaagcct
    35881 attattacgg tggataaaga aggaaggctg acgaatgttg acaaagttcg aggaagaaac
    35941 aagtcgatcc agacaatcgt caatgagacg gtaaaaacaa tcgttgcacc acttgatcaa
    36001 atcgtttatg tggcttacgc tggggatcta gagagcgcgg agaaggcaaa acggcttttg
    36061 gaagagcaga taaagatgaa agatgtgaag ctttatccct taggaccaac gatcgccagt
    36121 cacacaggct atggctgtat cgcgattttt tcgatgggaa tcagtcgata aaagcagtaa
    36181 ctcattctgt aaaaaagccc agaaattgat tgtaacaaaa acagcgactc ttaaaagata
    36241 gatgagcatc ctatctctta gaggtcgctg tttttttatt ttttaagcta aataggccat
    36301 tatgcccata actcgttcaa ttgtttttga atatctgcat tttccaaaaa ttcatcatat
    36361 gtcgtatcag ctcgatcgat cacaccttta tccgaaacgg caatgatgcg gtttgccaat
    36421 gtttggataa attgatggtc atgtgacgca aataagattg agccagtaaa tgccatcaag
    36481 ccgtcattca atgctgtgat tgattctaag tctaaatggt tcgttggatc atctaataca
    36541 agtacgttag ctttcgaaag catcattttt gaaagcatgc aacggacttt ctctccacca
    36601 gataaaacat tcactggttt caatacttct tcaccagaaa acaacattct tcctaagaag
    36661 cttcttaaga aggtgttatc atcttcttct ttacttgcat attgacgcaa ccagtctaaa
    36721 atcgttagat cagtatcgaa ttctttcgtt gtatcctttg gtaaataaga attacttgtt
    36781 gtcacacccc aacgtactgt tccagtatct ggcgtaattt cacccatgat aactttgaat
    36841 agcgttgttg ttgttatatc attttctgcg acgaaagcga ccttgtcttc tttgctcaaa
    36901 ttaaaagata tatcatctaa aattttcttt ccatcaatcg ttacacttac attttctacc
    36961 tgcaataagt cgtttccaat ctcacgttca ggtttgaatc caacgaatgg ataacgtcta
    37021 gaagaaggtt ggatatcgtc taaagtaatt ttttcaagca tttttttacg tgaagttgct
    37081 tgttttgatt ttgatgcatt cgcactaaag cgggcaataa attcttggag ttctttgatt
    37141 tgttcttctt ttttggcatt tgcgtttgct tgaagtttag ccgctagttg actagattcc
    37201 aaccaaaaat cataatttcc tacataaagt ttgattttac caaaatctag atccgccata
    37261 tgtgtacata ctttgttcaa aaagtgacgg tcatgggata caacgatcac agtattttca
    37321 aaattgatca aaaattcttc cagccaattg atcgattgga tatccagacc gtttgttggt
    37381 tcgtctagca aaagaacatc aggtttacca aatagtgttt gtgctaataa cacttttacc
    37441 ttttgtccac cagttaattc actcatttgc tgatggtgca attcttcagg gatgttcaga
    37501 ccttgcagta aaacagctgc ttctggttca gcttcccatc cattaagctc agcaaattct
    37561 ccttctaatt cagctgctcg aataccatct tcgtcagtga aatcctcttt catgtaaatc
    37621 gcatcttttt ctttcagcac ttcatacaga cgcttgtgtc ccatgatgac tgtttcaagt
    37681 acagtatagt cttcatagtc gtaatggttc tgtttcaata cggccatccg ttcatctggt
    37741 cccatagtaa cggaacctgt ggaaggctgc agatcccctg ataacacttt aaggaacgtt
    37801 gatttacctg caccatttgc accaatcaag ccatagcagt ttccgggggt aaatttaata
    37861 ttcacatcat caaatagttt gcggtctgaa aagtgcaaac tcacatcatt tacagtaatc
    37921 aatttttttc ctcactttta tcattattag ttttgacaag tcttatagaa ttatataggg
    37981 gaaaggtaaa agattcaagg caatcgaaca ttctttgtgt ttcacgtgaa acagaatgaa
    38041 aaaaataaaa ataaaagcgg agcgaaaata gctactatag gatttattag tttgcaacag
    38101 cataattttt gacgttattt ataagaggga gtactataaa actgtaaaag aaatgaaaga
    38161 aggattgaaa atggatgaaa aaagaaccat cgactggggc tcattgattt tgggaatttt
    38221 atttgtactt gtctcattgt tatcatttca agaccctgtc ggaaatttag ttgcaattgt
    38281 agtagtcttt gcgatttttg cctttattaa agggatattt gaactgtttg tacggaacag
    38341 attaaaagag ctaacaggat ataaaggaaa gatgccctta ataatcggga tcatcgatat
    38401 tctagttggc gtctttttct tattcaatat tggtgcagga gtcgcagcat tgccttttgt
    38461 ttttgcagta tggtttattg ctgattcagt tttagcgtta tttactgctg acttggcaag
    38521 aggagtaagt gaaggatatt attggtttac gatcatcgtc aatattttag gaattttatt
    38581 aggaattttc ttattgttta atccaatttc ttctgcgttg acattaagct ttttagttgg
    38641 attctatttt atgttatttg gtatcacaca tatcgtctat gcatttagat aagtaaaatc
    38701 aaacaaataa aaaagccggc ttccgattac agttttactg ttgtaattgg aagccggctt
    38761 ttttatcatg gacaaatatg cacgaggtga tttttaggtg tcgagacaaa tttatgcaaa
    38821 aatgtgagaa gctatataca caaattttat cttaatactc gaagcatagc gcagttgtca
    38881 caacctcttt tcatataatt cattcaatac tggcaaatca tggacttcat atttttcttt
    38941 gaatgcttgg atttcttctt cagaatattt tcttggatct aaatgcagat cttctactgg
    39001 caatgggcgc ttgttattga tcttcacatc gattactaca ggacgatctg attttgcagc
    39061 tgcatcaaag gcaaatcgaa gttgcgagta ttctgtgact gtaaagctat ttgccccgag
    39121 cgcttctcca actttaccga aatctgcatc ttctaaaaat acaccaaatt tcttttgttc
    39181 cgtatcttct tgttctgctt cgatgaaacc aaaagaatca ttcgaaaaga caacattaat
    39241 aattggaagc tgatatttga cttgtgtaat gatatcttgc atgaccatag caaacccgcc
    39301 atcaccactt aaggtaaata cttgtttttc tgggtagctt agttgtgcgg caattcctcc
    39361 tggcacacca ttacccatag ttgcgaacca tccagatgtc gtatgttttt gtttaccgtt
    39421 catattcaat aaacgaatag aatggatagt cgtgttaccg acatctgtga cgaaaatcgc
    39481 atcgtcttca gcaatacgat tgatttcttt atagacagcc tctggacgaa tcggttcttc
    39541 ctctcgatct tcaaaagatt tcaaccaatt tacccatttt tctttatttt cttgatttgc
    39601 taggtaccag ctatcagcag ggcgagcatc ccctaactcg acaagtcttt gcaaagctgt
    39661 gtttgcatca ccgaggatcg acaaagatgt ttcatggcgt cgtccaaatt ttgacgcatc
    39721 gatatcgatt tggataaatt cagcagttgg attaaaaaat gcacgtccaa atgggaagtc
    39781 gctaccgaca aataagatca gatcggcttc tgccaatgct tcatttccgg gtttagtagc
    39841 gacacgtcca gcaaaaccaa gaaagttttc ataatcatca ggaataattc cttttgctag
    39901 aacggaagcg acaacaggaa ctgaaaaatg ttctgagaat tctttgattt gcggaaatcc
    39961 gtttcgcgtt ccttgaccaa cataaagaac tggttttttt gctttttcaa gataaggcaa
    40021 agcagctagc aaatcttttt cttctggtaa tatcacactt gttttgtgag tgtgagcggt
    40081 ggagaaaggt tgttcgtcaa tttcttcaaa accaagatca acagggatag tcacgatagc
    40141 cacgccttta tgctcatagg cggctttaat ggcttcatct actacatgcg gaagactttg
    40201 tggtgtcatg actgtacgat tgtatacgct aacatctgcg aaaataggat tttcatttag
    40261 ttcctggaaa gagttatagt tcatggatgt agaagcaact tgtcctaaga acgctactag
    40321 aggcacatgg tccatctgtg catcatacaa accattgatc aaatgtgtcg caccaggtcc
    40381 tgcagagccg aagacagcgc cgactttccc cgttaatttt gcatctgcag cggctgcgag
    40441 tgcgccgact tcttcatgac gaacttgaat gtagttgatt tcggtttttt cttgatatag
    40501 cgcatccata attgaattga aagatcctcc gggaatgccg taaatatggt cgatacccca
    40561 gctttcaaga acttttacca ttgcgattcc tgcattgatt tttgacatgg tgtttcactc
    40621 ctttagcaat gatgatacat aaattttttg tatctagagt atagaacttt ttatcgaaaa
    40681 tcccaactaa ttcgtctttt cacaaaaatg tagtcatgtt tttgggaata tgctagagta
    40741 gtacatagat agattcaaga gaggggaatg gcatggttga atggctattg agacttgaac
    40801 cttggcagca agcacttgct gggacagctt ttacttattt tatgacagct cttggtgccg
    40861 ggttggtttt tttctttaaa gaaatcaaaa aagaagtgct aaacttgatg ttaggatttg
    40921 cttctggtgt catgattgct gcaagttttt ggtctttgtt agatccggct attacaaaag
    40981 cagaagaaaa tggagatatt gcttggctag ttgtaagtat tgggtttggt ttaggcggtg
    41041 tgtttttata tatggcagat aaaacactgc cccatatgca ttttggacca caacatgaag
    41101 cagaaggatt gcctacccat ttaaaacgga cgatcttgct tgttttttct attacattgc
    41161 ataacattcc tgaaggttta gccgtggggg tagcattcgg tgcagcagca actgcggata
    41221 atccgacagc cgctatatta gccgctgttt cggtggcgct aggtattggt atccaaaact
    41281 tcccagaagg agcagctgta tcaatcccgt tgcgacaaga aggtctgagt cgtaaaaagg
    41341 cctttgttta cggacaagct tctggaatcg ttgaaccaat tgctggagtg attggagcgc
    41401 tactagtaac gaaagtggag ctgctgcttc cttatgcact tgcttttgcc gcaggagcaa
    41461 tgatttatgt cgtggtggaa gaattgatcc ctgaagcaca gcagacattg tccagcaaac
    41521 gtcattttgc agtttttggc gtcatgagtg gatttatcat tatgatgatt ttggatgtgg
    41581 cattaggata aataaaaaaa atatatatga tatatcaaat agggccgcga acttatatcg
    41641 cgaccctatt tttttagtaa gttatatcat aatgaagtga atggtatcat acattaataa
    41701 tagttttttc tatgtgtatt tcctttgatc cagcgaacaa gccagatgat aagtgcgatt
    41761 gggattagaa ctttgaccgc aaaccataaa agcccgctta ggacactgac aaaaagactg
    41821 atgacgaagc tagctgcaat tagcactaaa agaaccatga agatatttaa gaacatattt
    41881 ttttgacgca tacaaaaccc tcctcttgtt cattctttac tatagaaaaa gtataacagg
    41941 gattgtttaa aaacacctcc aactgaagac tgatttttac ttcatctact taaatgatca
    42001 aacaattgct ttttctctgg tttatcatct aaaatagagc ggaaaaggag gagaatatca
    42061 tgcctaaaaa tcgtcttgaa gcatttacag atgctgtaat cgcgattatc atgactattt
    42121 tagttttaga acttcatgaa cctaacggag acacgtggga ggcgctcgct gcattaggtt
    42181 accgattttt tatctatatt gtcagtttta tcattttagc gatttattgg aacaatcatc
    42241 atcatttatt tcaagttgta cataagataa atggacgggt cttatgggcg aacagtttct
    42301 ttgtttttgc gctttcgctt tttccgtttg caacttcatg gatcagtgag tacatcaata
    42361 gcctggctcc agaaatgact tatggttttg ttaccttgac cgctaatatt gcgtattata
    42421 tcttggcaaa agaacttgtc aaagcaaata aaggccacga gaaagcattg aaggcgatgt
    42481 tgaataatta caagaaatcg tatctatcga ttggtttgaa tgtgttagga attgtcttag
    42541 ggtatttagt agcgccagta gccgtgctga tcgcaaacat cgtgattcta gctgcttgga
    42601 tcatcccaag tcgtaccatc gaatcacaat atcggtaaaa tgatccgtgt ttaaaattta
    42661 agagaaaaat atagaaaaaa taaataaacg atcaaatatc cgagtgatac tagtattcaa
    42721 agagggatgc agatggatca aaaaagaatt catgtaggac tgattactag ttttgtcacg
    42781 atgcttattt atttggatca aggtcatttg acttggacag tagttggtgt cgtaggattt
    42841 ttgacattat tcacagcaat aaaaaaataa acatctttat gtaatctgat gttctatcta
    42901 tttaagaaaa gcatgtttac agcagtatga ttctgttgta aacgtgtttt tatttgtgga
    42961 aaatttaatt tttagaaaag tgtatcgaaa aatagatagt tagtttttcc acagtggata
    43021 acttcgtaag agataaaaat aaaaaattag tcaaagaagg gcgtatagat ttcttttgat
    43081 aggagatata caacagaaat gaataaatat taacgtgttt tcaaacagta taaaacggtg
    43141 tggtttttgg aataaaaaaa taacatatct cattttacac ttttgttatt ttatacttaa
    43201 aaaataacga cacagaaaat accgtgtctc agatttct
//
LOCUS       DACDEG010000015        43221 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.015, whole genome shotgun sequence.
ACCESSION   DACDEG010000015 DACDEG010000000
VERSION     DACDEG010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.015"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..765
                     /locus_tag="IV142_000887"
     CDS             <1..765
                     /locus_tag="IV142_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8484562.1"
                     /translation="MFDIAEILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALS
                     SKELTNWIEAVNDDFNNHLLTFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRL
                     KHTLDKYQRKKFTKGRKDTVIQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTY
                     TKPSGEVNCPVHADYLNYRINSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISN
                     ALTHSDISTTKIYVNTPDIVDKAVFEAFQRGLNKCD"
     gene            complement(853..1245)
                     /gene="rpsI"
                     /locus_tag="IV142_000888"
     CDS             complement(853..1245)
                     /gene="rpsI"
                     /locus_tag="IV142_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP8484563.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(1261..1704)
                     /gene="rplM"
                     /locus_tag="IV142_000889"
     CDS             complement(1261..1704)
                     /gene="rplM"
                     /locus_tag="IV142_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP8484564.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(2223..2690)
                     /locus_tag="IV142_000890"
     CDS             complement(2223..2690)
                     /locus_tag="IV142_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP8484565.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(2810..4348)
                     /locus_tag="IV142_000891"
     CDS             complement(2810..4348)
                     /locus_tag="IV142_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP8484566.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVIGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            4428..5114
                     /locus_tag="IV142_000892"
     CDS             4428..5114
                     /locus_tag="IV142_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP8484567.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(5315..5842)
                     /gene="msrA"
                     /locus_tag="IV142_000893"
     CDS             complement(5315..5842)
                     /gene="msrA"
                     /locus_tag="IV142_000893"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8484568.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(5926..6603)
                     /gene="pgmB"
                     /locus_tag="IV142_000894"
     CDS             complement(5926..6603)
                     /gene="pgmB"
                     /locus_tag="IV142_000894"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP8484569.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(6600..7730)
                     /locus_tag="IV142_000895"
     CDS             complement(6600..7730)
                     /locus_tag="IV142_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8484570.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(7730..9913)
                     /locus_tag="IV142_000896"
     CDS             complement(7730..9913)
                     /locus_tag="IV142_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP8484571.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(9910..10935)
                     /locus_tag="IV142_000897"
     CDS             complement(9910..10935)
                     /locus_tag="IV142_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8484572.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(10980..11807)
                     /locus_tag="IV142_000898"
     CDS             complement(10980..11807)
                     /locus_tag="IV142_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8484573.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(11804..12871)
                     /locus_tag="IV142_000899"
     CDS             complement(11804..12871)
                     /locus_tag="IV142_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP8484574.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(12897..13733)
                     /locus_tag="IV142_000900"
     CDS             complement(12897..13733)
                     /locus_tag="IV142_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8484575.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(13730..14599)
                     /locus_tag="IV142_000901"
     CDS             complement(13730..14599)
                     /locus_tag="IV142_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8484576.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(14669..15952)
                     /locus_tag="IV142_000902"
     CDS             complement(14669..15952)
                     /locus_tag="IV142_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8484577.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(15966..17651)
                     /locus_tag="IV142_000903"
     CDS             complement(15966..17651)
                     /locus_tag="IV142_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP8484578.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            17926..18951
                     /locus_tag="IV142_000904"
     CDS             17926..18951
                     /locus_tag="IV142_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8484579.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(19546..23199)
                     /gene="rpoC"
                     /locus_tag="IV142_000905"
     CDS             complement(19546..23199)
                     /gene="rpoC"
                     /locus_tag="IV142_000905"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP8484580.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(23278..26895)
                     /gene="rpoB"
                     /locus_tag="IV142_000906"
     CDS             complement(23278..26895)
                     /gene="rpoB"
                     /locus_tag="IV142_000906"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP8484581.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLDELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            27623..28591
                     /locus_tag="IV142_000907"
     CDS             27623..28591
                     /locus_tag="IV142_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8484582.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            28609..29514
                     /locus_tag="IV142_000908"
     CDS             28609..29514
                     /locus_tag="IV142_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8484583.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            29511..30317
                     /locus_tag="IV142_000909"
     CDS             29511..30317
                     /locus_tag="IV142_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8484584.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            30474..31442
                     /locus_tag="IV142_000910"
     CDS             30474..31442
                     /locus_tag="IV142_000910"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP8484585.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(31759..32283)
                     /locus_tag="IV142_000911"
     CDS             complement(31759..32283)
                     /locus_tag="IV142_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP8484586.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            32570..34381
                     /gene="scm"
                     /locus_tag="IV142_000912"
     CDS             32570..34381
                     /gene="scm"
                     /locus_tag="IV142_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP8484587.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(34536..35522)
                     /locus_tag="IV142_000913"
     CDS             complement(34536..35522)
                     /locus_tag="IV142_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP8484588.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(35545..36111)
                     /locus_tag="IV142_000914"
     CDS             complement(35545..36111)
                     /locus_tag="IV142_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP8484589.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(36125..36541)
                     /locus_tag="IV142_000915"
     CDS             complement(36125..36541)
                     /locus_tag="IV142_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP8484590.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(36998..38932)
                     /locus_tag="IV142_000916"
     CDS             complement(36998..38932)
                     /locus_tag="IV142_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8484591.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            39234..40169
                     /locus_tag="IV142_000917"
     CDS             39234..40169
                     /locus_tag="IV142_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP8484592.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            40259..41362
                     /locus_tag="IV142_000918"
     CDS             40259..41362
                     /locus_tag="IV142_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP8484593.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            41375..42328
                     /gene="menA"
                     /locus_tag="IV142_000919"
     CDS             41375..42328
                     /gene="menA"
                     /locus_tag="IV142_000919"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP8484594.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
BASE COUNT        12501 a         9132 c         8132 g        13456 t
ORIGIN      
        1 atgttcgata ttgcagaaat actaaattat atcgaattta atcgaactac taaaattatt
       61 caaagtatca ctgcacctaa aaaaaatgct ttagaagaaa aaagaataca agaaggtaag
      121 caagcactat catcaaaaga acttactaat tggatagaag cagtaaatga tgattttaat
      181 aatcatttac ttacttttca tgattacaca ctttttatga ttaccctgta tcttggtgat
      241 agaaaaagtg agacttacgc tttgcaatgg aaatatattg attttgaaaa acaaacggtc
      301 cgtcttaaac atactttaga taaatatcaa agaaaaaagt ttacaaaagg tcgaaaagat
      361 accgtcattc aagtaccaga agttgtaatg acattgctaa gtgaatggaa atcggtacaa
      421 gctgatcaat tgttgaaatt aaaaattaag caaacgttag atcagtatct ctttacttat
      481 actaaaccat ctggagaagt aaattgtcct gttcatgccg attacctaaa ctatcgaatc
      541 aattcaatta aaagaaggca tcctgactta gttcatttat ctccacacaa actccgtcat
      601 acttatgcaa ctatagctag acaaggtgga gcagatatga atcaaatttc aaatgcttta
      661 acacactcag atatttctac aacaaagata tatgtgaaca caccagatat agttgataag
      721 gctgtttttg aagcttttca gagaggattg aataagtgtg attgatatgt gttcttaaat
      781 gtgttcttaa aaacaaaaga agcactttcc aaataattga aaagtgccag aaacgttgat
      841 atataaagga tattaacgtt ttgagaattg tgaagcttta cgtgctttct taagacctgg
      901 ttttttacgt tcgaccatac gtgcgtcacg agtaagtaag ccagcacgtt taagagctaa
      961 acggaagtca ggatcaactt ctagcaatgc acgagcgata ccatgacgga tagctcctga
     1021 ttgtccagcg tatcctccgc cgtttacgtt aacaaatacg tcatatgcgc cttttgtttc
     1081 agtaacagcg aaaggttgat tgattacttc acgcaagtca gcatgtggaa tgtattcttc
     1141 aacatctttt ttatttacag taattttgcc agttcctggt actagacgta cgcgagcaac
     1201 tgcgttttta cgacggcctg tgccgatata ttgaacttgt gccaatgaaa ttccctccta
     1261 ttagattaag tttgtgatgt ctaaaacttc tggttgttgt gctgcatgtg gatgttctgc
     1321 tccaccgtat acgttcaatt ttgtaaattg tttgcggcct aaagtgtttt taggaagcat
     1381 accttttaca gaagtttcga tcaaacgacg agaattttta gcacgcaatt cgccagcagt
     1441 gattgatttc aatcctcctg ggtagttgct gtgacggtaa taaattttgt cagtcgcttt
     1501 tttacctgtt aatttcactt tgtctgcatt gatgacgatg acgaaatcgc cagtatccaa
     1561 atgaggtgtg aaagttggtt tatttttacc acgtagtaca gatgcaacaa ctgttgaaag
     1621 acgtcccaat ggaacatcag ttgcgtctac tacataccat ttacgttcta cttcgcctgg
     1681 tttggccata tatgttgtac gcacgttatt ttcctccaat ttaagttctg atgtttgaca
     1741 tacacttcga gtttccgggg ctctttgtgg ggcaaacaat accatttaac atattacccg
     1801 ctaatctagt caatgtcaac gtttttctgc ttgattgaaa aatttatatg aaactttact
     1861 tcttttcttt cttttatata aaagggtcgt ttcctgtcaa aatgaaaaac aaaaagaggc
     1921 tgtgacaaaa gtcttgtcac aaccccttat tttgaataaa cggtgttctc ctgccaactc
     1981 ctcggtcaca agtcacaata tccataaaac ataagaagct gttttttaaa attgtttctt
     2041 gtgactcgga gctgaacggc aggcttacaa cctcttggta aacggtgttc aatcagctga
     2101 ccctcggtat tagctcaaaa aacggcaaaa tatgaggaac tattttgacg tttgtttctc
     2161 taatactcag gtcaaaacgc tgatttcaca acctcttttt cggtcaagtt caacaacagt
     2221 ttttacgcgt cgatctctgg cgctttgccc aaatgagctt ggatcatcca gatacgtttt
     2281 tctgtggctg ttttgaattc gataaagata tcttgtgttg gatcgtcgcc ttcttctcca
     2341 gaaacttcga ttcctttttg gtaaagatct gctagataac gatagcctgc aacaagtgtt
     2401 tccaaacgtt ctggaatagt acgatcccag cggccaattt catcagggat ttttgtgtta
     2461 tctgcaaatt cacgcaatgt agaataaggt gaaccgtcta atgtgatcaa gcgttcggat
     2521 acttcatcta actgagcgtt caaatcatcc atgaactcat ccatcattgg gtgcaatgta
     2581 aggaaacctg gtcctctcat gtaccaatgt gtttggtgaa caacaacaga catttggctt
     2641 agatctgcaa ctaattgatt taatacttct ttggtttgtt ggaatttcat ctttcttgcc
     2701 tcctaacatt ttcttacact taaaataata gtagactttc aatgaaaagt acaacaaaat
     2761 catttacctg agaacgattt tcagttcgct tttttatccg taaagccaat catttttcag
     2821 aatcagctaa ttcttctcgc agttgagcaa attgctgata agtctgtgct aaatgctttt
     2881 gtgtcgaaac aaataatgga tagattttct gatatttttt atgatcagtt tcttgtactc
     2941 caacttgctc tttcaattct atcaattcct gtgcttcact gatactttgg atctttccga
     3001 tgcttctcca gcctaaaatc acagctccta atgcggatgc ttctgtgaca gtagggaaaa
     3061 caagcgggta acccagtaca tcagccatca tctgacgaaa aatcggagat tgagcaaacc
     3121 cgcctgtagc acggatctct gtgagctgat tttcctggca aactcctttt aaaatccgat
     3181 aaaggttgag acagatccct tctagaatcg ccttcatcat ttctttttct gtatgttgcc
     3241 gctgtaaacc gataaaactg ccaaacgcat ctgcgtccca aagcggtgct cgctcaccaa
     3301 gtaaataggg atgaaaaatc agaccattgg cacccgctgg cacaaaagcg atagccgaaa
     3361 gcagtgcatc atatggattt tctccttttt ctttagcaga ttgaacgact tcctgtaaaa
     3421 atgtatgccc tgcccaatca aagatgccag caccattgct agtcgctcct cccactaccc
     3481 aatgtttctg atccagaaca taacaaaacg tctccatttc tggatggaac tgaggctgag
     3541 tagtaataaa acgaagtgct ccgctcgttc ctacggtcaa agcggcgatc gtatcaccaa
     3601 aagcacctaa ccctagatta gaaagcggcc cgtctgcccc tcccaaaaca aatgatatat
     3661 catttggcaa acctagttct gctgctgttt ttgatggtag acctattact tgataggtgg
     3721 aaggaaagac ttgcggcaat tgctctattc gaatgttcaa gtagtcaagc acttcttctg
     3781 cccattggaa ttcatggata tcaaaatatc cagttcctga agctgttccg tagtcgctga
     3841 tcagttcgcc aaacaatcta taaaagatat agtctttgat accgacaaaa taagcagttt
     3901 ttttgaataa ttcaggttgg ctgttttgca gccacttgat tttgttcaat ggtgtcattg
     3961 gatgaatcgg cgtcccagtc tgccgataaa attctttgcc aagcacggta tatttcagtt
     4021 tttctgcttc gtccgctgct cggttatctg cccaagtgat cattcgagat aacggtgttt
     4081 tctcttcatc caacaatatc aggccatgca tcgcagagga gaaggagaca aattgaatga
     4141 ttgtgtctga aggcaactgt ttaattactt cttttataac ggtgcagaca ccttgaaaga
     4201 tcaattcctg atcttgttct gccatccctt gtgtttcttg gatcaaaggg tagcgaaaat
     4261 aagcggatgc tgcttcttgg gcttcatctg tgaaagcaac tgctttggtt tgtgtggttc
     4321 cgatatctaa tccgattgaa acgttcattt tttcacctca aattttatgt atgtacaaaa
     4381 atttagttta agcttgcgta tcttggtttt gaaaaggggg agatggcgtg ttactttatt
     4441 ttatcattgg ttgctgtatc ggatcatttt tatgtttaac cgcccagcgg attccgttag
     4501 gtcactctat tatttatcct agatctcatt gtgtcaaatg ctgtcgttcg ctttcttggt
     4561 atgaattgat tccgattctc tcgatcatcg tccaacgatt tcgctgccgc tattgcaggt
     4621 gtcggttgcc cttctactat ttgttggcag aagccttatg tggtggtctt tttgcctggt
     4681 tctttacttt ttcttccgca agaaattttt cgacgttcat atggatgctt tcggctttgc
     4741 tattttcgtt gatggattta ttttacttca tggtccattc tcacacgcta ttctgttctt
     4801 gggcaatttt atggattttt tggttgcagg ccggcgtctt ccaatggcaa agtgtgctgt
     4861 taacgtttat cgtaggcggt gtgtgccttc gctatggtca atcctatctc ggagctggcg
     4921 atactctgtt gatgctttct tggagtggtg gcttgtctct agaagagttg cttcagatct
     4981 tgtttcttgc tagtctgttt ggaatatccg ttttttctgt ttactttatc tgtcataaaa
     5041 aaaagttgga aaagctgcct ttcattcctt ttttgagcag tgcgttgctg attgtccttc
     5101 atcttaggta ttgaaaaaag gttgtgacaa acgacttgtc acaacctctg atatatagtg
     5161 ttctcttacc aaatccttgt gacttggagc taggcgacag gttcacaacc tctccactaa
     5221 atggtgttca atcagctgac cctcagtatt agctcaaaat gctggtttta caacctctga
     5281 tatacggtgt tccataagta gctttttcgg cttcttattt cttgacgccc caatgtcgac
     5341 gaaggaaacc tgcacgtcca gaaccttctc gataaagatt gtaatggagc ttgtttttct
     5401 tgtaataatc ctgatggtaa tcttctgcag gataaaattc gcttcttggt tcgatttttg
     5461 tcacgattgg cttatcgaat cgcccgctgt cttgcaatgc ttgttttgaa cgctctgcaa
     5521 tatttcgttc ttcttcatca ttatagtaga tcactggacg ataagaatca ccgcgatcag
     5581 cgaactgtcc taatgcgtca gtcggatcgg tttgctgcca atagatttcc accaactgat
     5641 cataagaaat aacttctgga tcatattcga tctctactgc ttctgtatgc cctgttgtgc
     5701 ctgttgttac ttgttcataa gttggatttg gtacatgccc gccagtataa ccggatgtca
     5761 cagaaatgat ccctggctgc tcttcaaatg gtttgaccat acaccaaaag catccgcctg
     5821 cgaaaactgc tttttttgtc atataaaatc cccctttttt ctcatcatac cttgatttcc
     5881 aaaaggagga taacgttttg cttgtgacag aaaaaacgga aactattata tacgtgatac
     5941 ggcttctgat aaatgccaaa acgaacgtac ttgatcaaaa acggttaaat caggaatcac
     6001 atattcagca gaaggaagat ttatgttttc acctattccc aatacatgca tgttaccagc
     6061 caaagctgct tgacaaccag cttctgaatc ttcaatgacc aagcatgcat ttggcggtac
     6121 attcaattgc tgtgctcctt tgagaaaaac ttctggatca ggttttgctt ttgatacttg
     6181 cgtgccgtca ataaggacat caaaataact tgtcaaattc gttttttcta aaatgaggcg
     6241 tgcatttttg cttgctgatc ccaatccgat cttgatgttt gtttttttca agtactcaag
     6301 aacagctatt gcaccaggca aaacggatgt ttcatccatt ttttgaatcg cttgaagata
     6361 tagattgttt ttttgctcag caaaggctga tttctctttt tctgagaatt tctgttccat
     6421 ttttccaaat gacagcaatc gctctagtga ctcggttctg gatattcctt tcagttgctc
     6481 gttttctttc tcagtgaatg gaatggacag ctggttagct aaaaccagcc acgcttcgta
     6541 atgataacga gctgtgtcta caagaacgcc atctaaatcg aatagtgctg ctttgatttt
     6601 catcgattac ctccttgaaa atgaacgact tgaagtaaag atgcgatgtt tttagctgtg
     6661 cgatgaagat tatcagtggc ttgctgtaaa gcttcttgta aaggcacagc tgcaggaagg
     6721 atcggaaaaa cggccgaaat gccaacatct gctgcttttt cgcttccttt accaacactt
     6781 ccgcaaatag ctatcacagg aacgccctct ggtactcttc tagcaatccc aactggcgct
     6841 tttcctttta gactttgtcc atctagacgt ccttcgccta cgattacgag gtctgcttgc
     6901 tgtacccgtt tatcgaactc cagtaaatcc aatacgtagt cgatgccaga gactattttc
     6961 cctgaagcaa atgctaacag tcctgcaccc attccaccac cagcactact gcccgatgcc
     7021 tcgattactt gaggggcaaa tctcatataa aaatcatgca tggcttggtc agccactgct
     7081 aattcatttt ctttcaagcc tttttgtggt ccgaaaacaa aagaagcgcc ttcttttccg
     7141 cataaaagat tagtaacatc agtaatgaca gtgattttgg ctttaatcag ctcaataggc
     7201 acgttcgttt tatcaactgt agcaatttta cctagatttt ctcccagtgc ttctactttt
     7261 tgattatctt gatcgtaaaa agcataacct agaccttctg ccatgccgat gccgccatcg
     7321 ttggtactac ttccgccaac gccgatgatg atgtgtcgaa caccttgttg caacaattgt
     7381 cgtatcagtt ctcctactcc tcgcgtggat aacctaagtg gctgttgttg gtttagtggg
     7441 acatctttca atccgacgat ttctgccatt tcaaaaatcg cagtttgacc gtctgaagcg
     7501 tactggattt ctttgttttt gccaaatgca ttagtaacga ctgctgtttg ccgcttcaac
     7561 cttaaactgt ctgctaatga tgaaaccgtt ccttctcctc catcgcctat aggtagcaga
     7621 tcatattcag cctgaacaaa aacttcttga aaacctgatt gaatggcttg tgctgcctct
     7681 aaggctgtca gactttcttt gaaagaatct ggcgcaatca gaatacgcat tattttcccc
     7741 tccgttccaa gatcagttct ttttccagct ggtattggtc tccattgaca aaaataggta
     7801 atgtttcttt cgtctctttt tttattacta tttgtttttt tgtcaaaaca aatagcaacc
     7861 tgtgattttt ccaaataaaa ggaaaagaaa ttttttccca tgctgaagga agtcttggag
     7921 aaatagaaag agttccattc gttactgtaa tacctgaaaa accaaatata actgctagcc
     7981 atagtgctcc taatgaagca gcatgaattc cgtcatctga tgaatgcgga tctggtccta
     8041 aatcaatcag gcacgcttct tggaataaat gatacgccca ttccatttct tggatacggc
     8101 aagcctctat ggcgtgaatc gctttactca acgatgagtc gtggattgtt ttgttttcat
     8161 agtaatgcaa attcttagaa acgattggtt gagaaaaata ctcaggtaat aagtataaca
     8221 gcatgacaag atctgcttgc ttgctgactt ggagttcatt tacttcgcta cgggcataat
     8281 caagtaaaat agactggctg ccaggacgtt ctttgtaagt ggtcaaatca ataactggct
     8341 tactgagata cgtgtcatct tgcggaatca gctgttcttt gttaggcact ggcaaatata
     8401 acttatctaa aaaattttga cattctttca aaaaaagcgg atcgccagct tgatatttaa
     8461 cagctaaggc cacattataa tgcgccagat agttcgtata tgcattattg tcgatatatt
     8521 ctgtgtattc atctggacca atgactctgt gtatctctaa atgatcatcg acttttacag
     8581 ctctgctcag ccaaaaagct gctgtttcat gaatgagcgt ttttcccttt gtttctaaaa
     8641 acgattgatc ctctgttgct tggacatact cttgaacggc ataagcaagg tctgcactga
     8701 tatgatgttc tgcggcagct gaagcaactt cttgtcttgt tccggttttg atattcaatg
     8761 cagcaaattt gggtgtctct tcctgaccag tgtgtgcgct ttcccatggg aaaagtgcac
     8821 cttgatagtg ataatctctt gctttttgcc gtgcgccttc taatcgatca taacggtaca
     8881 gcagcaaatt ctttgctact tcaggaaact gatataaaaa gaaaggcagg ataaaaattt
     8941 ccgtatccca gaaaacatgc cctttataac cttcgccagt tagtcccttc gcccctacac
     9001 taacacttgg atcattgcgg ggagtcataa tctgtaaatg gtaagcagcg aagtccatcg
     9061 ctaactgatc gaaagaagaa tcgccttgga tcgttatccg atgctgatgc caaaaatctg
     9121 tccaataaga catcgactgc tctaaaattt ctgtataact aacgcttttc tgggcttggg
     9181 caatactttt cttaaacggg tctgtttgct gaagagaggt agagagcacg atgatcttct
     9241 ccagcgtaaa ggacgtattt tttctgatct tttctgaaaa ggaagcggtg atccggcgat
     9301 tttttgcttg gaatacacct gctttattga atttagctgc aatcccaata tcaattttac
     9361 tttctgttgt ctggtaggat gcactgatca aatcgtcacc aaatactcgc agttcttttt
     9421 caaccaactg ctgtgtacca aaattggttt gctgtccatc gatacccgtt ttcacatcta
     9481 cttggcagtc ttcggtcaac ggcttgatga ccactttcag ggctgcctga tggagattat
     9541 tttgatcagc aacacgacaa gtaatgactt ccactttttt tccttgagca gtttcaatga
     9601 gcgcatagcg tttaagctct cccatttcta aatctagtgc ccgctcgtat gcaagaactt
     9661 tcatcgtctc aagagaaaaa tgttcaccgt cgatttttat ttcccatctg atgacatctg
     9721 ggagattcac taactcggct gattctgctc ctaaaggtcg gttatataca ccagctgcaa
     9781 acattcccct tacttgattc cgatagtctt cctcctggct tcctctgata ccaaaaaatc
     9841 catttccaat ggtcatctga ctcgcatact tattgatata ctctaaatca aactgttttt
     9901 cttctacttt cataaggtaa taacttctcc tgtttctgcc gaacgatata aattttccac
     9961 cagattctgg atgacatatc cttgccagcc atctgcaatc atctgagtct ggtcaccgag
    10021 cgcccgattc acaaaatgag ccatacttag ttcatgtggg tcattaactg gcgtggtctt
    10081 ttgtaaggaa accagctttc catctacatc ttcgaatagt tcgcctggat acagattagc
    10141 acctgcctga tcgccacaaa aagccacatt cagtttgtct tctctgatat tcaaagcaaa
    10201 cgaagtattc agttggatga tcttgccgtt ttctaattcc agtgtgccaa aaattgcatc
    10261 ttctactgtg aatttgcttg gatcccattt cccgaatttc cctgaatttt ttttcgtacc
    10321 aattttttgg aagctgtacg cccgaacgct ttttacttta ggaaatccag taatatacat
    10381 cgctgtatca agcatgtgaa tgccataatc gatcaaaggc ccgcctcctt gaagttcttt
    10441 gtcgataaac gttccccagc ctggaacacc agaccgacga agcgcattcg cttctacgta
    10501 ataaatatcc ccgagaacat caagatgatt cttgatcatt gttgcttcgt cagaaaaacg
    10561 atgctggaat ccataagcca aaatctgccc gtttttatcg gctgtctctg ccatctttct
    10621 tgcttgatca gctgtcatag ctggtggttt ctcgcacatc actgaagcat tagcttcgag
    10681 ggcagccaat acatgttcga agtgaaagcg attgggtgta cagacactca cgatatcggg
    10741 agaaatctca ttcagcattt gtgtaactga tgtatacgca tgagaaaatc ctgtacgtcg
    10801 gcaaaagtct tctgctcgct ggctgactgg atcaaccaca gcggccaatt ctaagacatc
    10861 tgatcttttt tgataataag cagcatgtac tttttctgct acttgtccag cgcctatgat
    10921 tgctactttc ttcatttatt tctccctcct ttgcttattt acttattgtt attctttctc
    10981 tattctttct ctaaatcatt taaatgacgc gctgtttcca gacagttttt caagtattgc
    11041 aatgattcct gataaatttt cacttcattt tctcctcgca aacgacattc atataccata
    11101 taaccttcat actcattttg aagtagcgtt tgaaaatggc gagtaaaatc aattgcgcca
    11161 gatcctggtt ggtagcgatg attatctgct aaatggatat gacccacata ctctttttgc
    11221 gtagtcaatg cttttgaaat atcatcttct tcaatattca tatgataaaa atcagcgatg
    11281 atctgcacat gtttcaattg attagagtca atgtaatgac gcgcatccgc taacaaattg
    11341 atcatatggt cttggtatcg gttcaaaggc tccaaataaa taaccgtacc tgtttcttcg
    11401 gctactttat ctaaataacg aagtgattcg ctgactgctt tatagtctcc ttctttgctt
    11461 cggggtgaaa ccatcggcgg taatcgataa gtgaacattc cccaagctgc tgggataacg
    11521 attcctttgc ctccaacctc gcttagtgca cgcaaaatct cagcaatttg acgtacacca
    11581 ttaagacgcc gttcttcgat aaagtcaccg atccacccat cgtagccgtt acaagctgtc
    11641 actactggga tccctgtttc tttgattgcc tgtttgactg tgtcgatatt ttctattaac
    11701 aacttaccgt cgatttcgta ttcttcaaat ccacagtctt tgataaatct aaatttttct
    11761 aaaatgtctc ttgggaaaaa tgcttgatct tgcgtaccga atttcattgt tctcctcctc
    11821 ggacttgaac gcccattttg atactttctt ctgggtgctg gtctacataa cgcatgtaag
    11881 cttctgcgct ttctgcaaat ggcacaactg gatcaatgat ttcttcacaa tccaagtaac
    11941 cgttcatcaa taattcccaa catgtgtcct cgattcgttt acggttccat cttgggtaat
    12001 ctgggtttgg ttcactagat gctcgagcaa agacgatttt tgcattattg aaatgtgctt
    12061 cgcgaccaag attaaaccca gctggaaaag gtttagcaaa ggcaacataa gaaatgatcc
    12121 cgccatagcc gatccctctt aaagaagctt gtaatgcggc agcactgcca ctcgtttcaa
    12181 taactgaatc tactccacgt ttatctgtca attcctttag acgaaaaccg acatctgctt
    12241 tcataggatc tagcgtgtaa tcggcaccgt atttttctgc taattctcta cgctgcgcta
    12301 tgggatcgat cccaacgatg gtagtagcac ctgctttttt agctaattga atcgcaattt
    12361 gcccgatagc gcctaaaccg aagattgcta cagcatcacc tgctcgaaca tgtgcttccc
    12421 ggataccacc taaagcgaat tgagcaggat cgtaacatac tgcatttttc cattgatcca
    12481 accgatcaag tttacgtagt ttataattat caacaccgtt tgtaatcagc gtttcacgaa
    12541 tgggtccata agtgcagact aactctcctt tttgatactc tgtcaccaaa ctgcccgttt
    12601 cttcgatcgt accgacaatc atatttccta attgaaattc gccaaatacc acaccgcgtt
    12661 ggcttccttc ttctcttggc aagaataggc gccattcttc tgaaaattct tcatcgataa
    12721 atggacttaa tcctctaaag tcgacaattt ctgttcctgg ttttggagta gcgaaaatta
    12781 ctcggatacg tacttcgttc ttttcgatct cacggtcttc gtaatctttg aatccggcta
    12841 ttcttggttc taatgcaact aattttttca tcttttcttt cctctttctt ttctttttat
    12901 ccttttacgc cgccttcgac taaatcacct ttaataaatc gttcagataa cgcatacata
    12961 atgatgactg gtaaagcggt caccaatgaa gcggccatca ttcgtcccca aatgtagtct
    13021 ggtgtactga ataatgtgtt caaaccgatt ggtaaagtaa atttttcgga ggtcgacagg
    13081 aaaatcgaag caaataaata atcattccac gaaaccataa agcaataaac gaagacagaa
    13141 atgattccag aaatagccaa tggtataatg atccgaataa tgatctggat ccggctaaga
    13201 ccatcgatca ttgctgcttc ttcaatatca gtcggaatcg tgtcaaaata actttttagc
    13261 atgaatatcg cagtaggaag tgtttgaacg accatcgtga taatcagtgc tgtattggta
    13321 tcatacaaac ctagaccgga aatgatttta aataatggaa caacgatcaa aataccggaa
    13381 aacatataaa cggtgtaaaa gctagcattg attttcgagc gagctttgaa gcgtagtttt
    13441 gacaaagcgt atgccccgct gatcccaatc actactgcag ttacggaggc tatcaaggag
    13501 atcactaaac tatttttgaa atacatgatg aatggaaaaa tctcagcatt gaaaatatcc
    13561 tgataatgtt caaaagtcca ttctttcgga aaaatagttt gtgtcgttgc aattgcttct
    13621 tgtgaatcct tgaatgatgt catcagcatg atcaaaaatg gaaacaaatt gacagctaat
    13681 agcaacaaaa tcgtcgcgta aaataagatt ttcctgctct ttttattttt catttcaagt
    13741 ccaccttctt tcgtgagagc aagacgaccg ctgcaataat gatgaacaat agaatcgata
    13801 tagctgctgc ttttccaaga tcattgaacg caaaggctgt ctgatacaaa taaacgccta
    13861 agatattgac tttattcgtc agtagataga catctgtaaa catatagaac atccagatag
    13921 atcgtaaaac aatcacagtt gctagtactg gtttgattgc cggcagtgtc acgatcttga
    13981 acttctgcca agcatttgca ccgtccattt cagctgcttc ataaagcgtt ttgtcgactg
    14041 tttgcaaaat agccaaaaat gaaataaacg catacggata gtatctccag atagcaaaca
    14101 aaacgaccat aaagaagcta gcaactggct ggtcgaacca cagcggtgct tcgttgaaaa
    14161 gctgcaacct atctactaac agatcattga cgattccata actattattg aacatatatt
    14221 tccaagcaaa aatcaaagaa atagatggcg taacataact taataaaatc agtgaacgca
    14281 ccgttttcct aaatctgaac ttgcggttaa gcaaaatagc tacggctaat cctaaagctg
    14341 tactgcctgc tacaactaag aatgtgtaag caactgttaa acctaaagaa cgataaaatt
    14401 cactatcact taaaatttca agataattgc ttaatccgat aaaaacggcg tctgagttag
    14461 gattcagcgg catatcgaaa aaactgatct gaatattgga aagcattgga atcagcacta
    14521 aaacagctaa taaaagcatg cttggtgcta aaagtcccat ggctaatttt aagtcagaat
    14581 gtttccttgg acgattcatt gaaacctccc cctgataatc tgccaatagc tatctacttt
    14641 tttcgaacac cattggcaga acttcttgtt atttcgtatt tttttgtgcg tcagctaaag
    14701 cttgatcgac atcttcgttt ccgacagtta cttggttcac tgcttttccg atcacacctg
    14761 agcttgtgat atctcccatt ttcgtgaaat tcttttcccc gactagtcca aagacttgta
    14821 cttcatcaaa agcactagca atctcagatg gcagctcgcc aaaagacttg atgacttcat
    14881 tttctttgta accatccagt tcgactactt gtttattcac tggttgagca ccaccaggag
    14941 acatcaagac ccacttttcc atgtttttcg gttgagataa gaaagctgtg aatgcttcag
    15001 cagcctcttt ttgtttctcg tctagaccag aagaaatcgt caatccagat actgtgccat
    15061 agacagcttg atctttttgt gttgggatag caaaaccgat attttctgag ttccccgctt
    15121 cataaacaga aggcaaaatg taagttgaat agattgccat tggtgctgtt ccattcatga
    15181 aagcatcttt gatttctgtc acatcattag agcctggcat cgtgtattgg gataagtttt
    15241 gataaaacga caatgcttct ctcatctttt ccgtatcgat cgttacctcg ccttcatcat
    15301 ccaaaacatt tgctccattt gacaaagcaa attgagagaa cgcttgctca ctcattgttc
    15361 cttcagcagt aggcatagca attccatatt gtttattctc tttatctgtg aaatgttgag
    15421 caatttttaa aatatcatcc caattttctg gttctgaaaa accagcttct gataattttt
    15481 ctttgtcata ccaaatacct tgaacccagc cgctaattgg tgcagcaata tagctttttc
    15541 catcttctga acgaacaagg tttttggctc cgtcataata attatcttct ccagcttcct
    15601 cgataacgtt ttggactgcc gtctgatcaa tcagttcatc tttatccatc actttagcaa
    15661 aatcctgact gacttccatt acttctggta atttaccgga acgagctaac gtaacgattt
    15721 ttgtattgaa cgctgattct tctaccggca cttgcttgat tttgatgtct ggattttctt
    15781 tttcaaaatc agcaaccagt tcattgatca cacttaatcg ttcctgttcg acagaagagt
    15841 gcatgaattg aatcgttgtc tgttgtgatt cgttagagct ttgactttca ccacaagcag
    15901 taagaagaaa tccagtagct agcagacagg tagtaacaat aatttttttc atttttttcc
    15961 acctcttatt ttattatttt cagccacata tattcatacg gctggaatat caactttcgt
    16021 tcttgactgc aatggataat ttgtccagaa aacaggtcta ggtaatctcc ttttggtaaa
    16081 agataacctg ctgattgatt ggaaaaatta tggataacta agactttctc ttccttcgct
    16141 tcgcgaataa aactgataaa agaatctgct gagtccagta agtaaatcaa actattcgga
    16201 tggaaggctt tttctttttt tcgaatagat agcatctggg tcaattcacc atatactgct
    16261 gttcttaatg gatctgtctg caactctttc tcaatatctg ctaacaaaaa tttttgccga
    16321 ttgatttttc gattctctcc tgttttttct acgcctttgt aatcatttcg acttcccagc
    16381 acactttgaa tataaattgc cggcacgcct ggtaacgtta ccaaaaatga atgagcagct
    16441 aaaaagcgtt tgcttctcca ttccgtcgta tctgttggag aacttaacgc atctagatac
    16501 gtagcgttta tctcatatgg actggtcgtt ccatctgggt ttttcttgta gtttactcgg
    16561 gtaccctctt tcttcagctg ttccactaat tcaaggatcg ttttttccgg cagaatacct
    16621 cgtaccggat tcaatccgat accgtcatgg gaggctaaaa aattaaaaaa agttgttttt
    16681 ttcgatgcga aatcgatggt ttttaaccat tctttcaact tacttgtatt acccgaacga
    16741 atcgcttgaa gggtcaaagg tggtagcgaa aactgataga ccatctgtgc ttcattctcg
    16801 ccattcccaa aataactgat attatctttg tgtggcacat ttgtctcagt gatcaatacc
    16861 gttcctggag ctgcttcatc agcgattgca cggaataatt ggatgatttt atgcgtttct
    16921 ggcaggtgta tacaagaagt tcctagttct ttccataaaa atcccacagc gtccaagcga
    16981 ataaatgaag cgcctttttc taagtaaaac aaaagaatat cgatcatttt caataaaact
    17041 tctggatttt taaaattcaa gtctacttga tcttcgctaa aagtcgtcca tacgtgtttt
    17101 tctttcccgt ttttgtcaat gaacgttgta agtaacgggc tggtccttgg tctagtcacg
    17161 ctgcttgtat ctgtatccag tggcaaatca ataaaaaaat ctttgtacgt atcattcaag
    17221 tttaaatatt cctgaaacca agtactttcg gcagacatat gattgcagac aaagtcgaac
    17281 attaaccgtg tttcgcttct catttcttct atatcttccc atgtgcccaa acggtcgttg
    17341 attgcctgat aatttacaac ggagaaacca tcatcagatg tatatgggaa aaatggcaag
    17401 aaatgtacaa ctctaaatgt atctgccaaa tacttttgat aaaatttctt aaatgtacgc
    17461 aaagttggtt gctgctcttc aaaaaattga tccccataag taatcaagaa aatatcttct
    17521 tcgtcccaat gcagtttgcc tgcctggcct ttcttctgct gataaacact gactttttgt
    17581 tcaatcatag aggcggtttt ctcagacaaa tctgagtcgc gataaatcgc ttgtaaaagt
    17641 tttgtataca tcggattccc ctttcttttt tgggagcgtt cccaacgatt tgatcatatt
    17701 atggaaaacg cttactgtca atagcatttt tttaaataat tttgatatac acaaaaatac
    17761 aaataagaaa gcttatgtat caataattaa tagcttctta aaaaaatatg gtctctcatg
    17821 atcatgcaag ctattcctaa attttcaaaa tcatagacaa aaaagaatgt ctattgtact
    17881 atggaattat tctaaaacgc tcccaaaaag gaggtgcata taaaattgaa acctactatt
    17941 tatgacattg cgaaaaaagc taatgtctca aaatcaactg tctccagagt attaaataac
    18001 cagccaaata tctctgataa agcaaaagca agtgtacttc aagcaatcaa agaattgaat
    18061 tatgaaccga gcaaaattgc aagaggactg tcaggaaatg gttttgacgc gattctagca
    18121 ttatctcatc gtacttctca tacgacctat ggcaatcctt ttttttcaga tgttattcaa
    18181 tccatttctg ccgtttctga acaaaataac ttcgacttga ttctacaaac ggcaaaaaca
    18241 ccagaagaag agctagaaaa atgcgcgaaa aagatccaag aaaaaatgat ccgaggaatt
    18301 attattttaa gctctacgat ggatgaaaca cttctttttg aattggatca atacaatatt
    18361 cctattgtgg taatcggaaa agtcggaaac gactacacga acatctactc agtggacacg
    18421 gataatttcc aggatagtta cgatttggtg gaaacactga tcaagaatgg acaccgtcat
    18481 atcggctgtc tccatgctcc gacagaagtt tatgtatcca atgatcgtct caatggttac
    18541 cgtcaatgct tatttgatca ccacctagat atccgtaatg aatggattat agacggcggc
    18601 tttcgcttag aagatagcat ggcagcaatg aatgatttaa tgaagctacg agaaagacca
    18661 actgcgattt tttctaccga tgcattaaaa tcattgagta tttacaaagt ggcagaacaa
    18721 tctggaaagc agatacctga tgatctttcg atcgcaggat tcagtgatcc cttattgtcc
    18781 tccctatttt cgccacagtt gactcggata gacattccga caaatcagtt aggaatcaaa
    18841 gccacagaac ttttattcca actaatccac caccaatacc ctgaagaaaa aagaatcatt
    18901 ctttcaaccg agattactct cacagattcg atccaacaac aagaaatgta gaacagaatt
    18961 ataaaagaac agaaacactg aggataagtc agttactctg ttcttttttc aatcactcat
    19021 ccctcgttac cccctcccgg tattttagcg aatttttctt attttccatc tgttttagat
    19081 agctgtggat aactataatg tttgtgttaa actatttttt ccacagggaa gaaaaaacta
    19141 tttttaacca cgttgtgtat aagtttttat agttcgtttt tttaaacaca tttaccctta
    19201 tttcaatcca tgttatttgg ggatacttat ctctaaaaca taaatagaat aacagacatg
    19261 tctactggtt ttgttttatt cgaggaatta ttcaataata gctagcaaaa tattaaaaat
    19321 aaccgctcta tcttcccatc aatttatttt gcattcaaaa aatgatctat ctttactttt
    19381 gttgattgac ttctcttgga agatgatcgt tccgatacct taattggata cgaaccgatg
    19441 ttgttaagat cctttcccac ttttcttcta gttagacaaa ttattttaga caaaccaaaa
    19501 aagaggttgt gacaataatt tgtcacaacc tcttttttaa aatatctatt ttgtttgatt
    19561 gttcattgcg tcttctgcag ccatttgcgc ttcaatatct gaaatactgt agacattttc
    19621 gcttgctaca ccgacttctt ttggttccat gtcacgataa cgttgcatac cagtaccagc
    19681 tgggatgatc ttacctataa taacattttc tttcaaacca agaagaggat ctttcttacc
    19741 gcggatcgca gcatccgtca agacacgagt tgtttcttgg aatgatgcag cagataagaa
    19801 actatttgtt tctaatgatg cttttgtgat acctagtaag acaggtcgag aagttgcagg
    19861 aacgcctcct gagactagcg tattgtagtt ttgatcttta aagtctgcga tgtctaataa
    19921 tgtacctggt aagatatctg tatcacctgg atccattaca cgaactttac gcagcatttg
    19981 acgaaccatt acttcgatat gcttgtcgcc gatttctacc ccttgcatac ggtaaacacg
    20041 ttgtacttca cgaagcaagt agttttcaac agataataca tcgcgaactt gcaacaattg
    20101 ttttggatcg attgatcctt ctgtcaatgg tgcaccacgg tggatcatat cgccttctgc
    20161 tactttcata cgtgctgtat aaggaacagt atacgtacgt gtatctgttt ttcctttgat
    20221 tgttacttct ttggtacgtg ttgcaggatc ttctgagata tcgatgacat cccctgttac
    20281 ttctgtgatc actgcttgcc ctttcggatt acgtgcttca aagatttctt ggacacgagg
    20341 aagaccttga gtgatatcgt ctccggcaac cccacctgta tggaatgtac gcatcgtcaa
    20401 ctgagtacca ggttcaccga ttgattgagc ggcgatcgta ccaactgctt ctccgacttc
    20461 aacgtcagag ccagttgcaa ggttgcgtcc gtaacaatgt ttgcagacac catgttttgt
    20521 attacatgtg aagacagaac ggattgtcac tgtttcgaca cctgcatcaa cgatttgttt
    20581 tgcgatgtct tcggaaatca attggtttcc tgcaatgatc aattcatttg tttcaggatg
    20641 acgtacttct ttacgggtgt aacgtcccac taaacgatct tctaatgatt cgatgatttc
    20701 atttccctcg cggatggctt ggatttcaag acctcggtca gtaccgcaat cttcttcacg
    20761 gatgattaca tcttgggcca cgtcaaccag acgacgagtc aagtaacctg aatcggcagt
    20821 cttcaaggct gtatcggtca tccctttacg agccccgtga gttgagataa acatttccaa
    20881 gacggatagt ccttcacgga agttcgaaat gattggcaat tccatgattc gaccattagg
    20941 agcggccatc aaaccacgca ttccggcaag ctgtgtaaag ttggaaatgt taccacgagc
    21001 tccagaatca gacatcatga agattggatt tttcgcttcc aaaccttcca tcagtttttg
    21061 ttggatgctg tcttttgcag cattccaaac agcaatgaca cgttcataac gttcgtcatc
    21121 tgtgatcaga ccacgacgga attgttttgt aatagtttca acttgtttat gggcattttc
    21181 aatgatttct tgtttttcat tcagtacact gatgtcagcg atccctactg tgataccggc
    21241 atgtgtcgaa tgtttgtacc ctaagtcttt catacggtca agcatcttag aagtttctgt
    21301 tactttgaat cgtttgaaga cttctgcgat gatgtttcct aagtttttct tcttgaacgg
    21361 taatactaat tcttgctctt tgatatgtgc aggaatatca gtaccagctt caacaaagta
    21421 tttgtccggt gtttggacgg tcaagttgaa gtctgttggt tcattcagat aagggaattc
    21481 aggaggcatg atctcgttga agatgatctt accaactgtt gtgatcatcg tacgttcttt
    21541 ttgccattca gtaaatggtt tttcacctaa tgagttaggg ttcacaccga tacgtgaatg
    21601 taaatgaaca tatccgttgc gccatgcgat aacggcttcg ttttgatctc tgaagaccat
    21661 tccttcgcct tcgcgtcctt cttcttccat tgtcaagtag tagttaccta gaaccatatc
    21721 ttgggaagga gtaacaactg gtttaccatc tttagggttc aagatgtttt gagcagctaa
    21781 catcaacata cgtgcttctg cttgtgcttc ttcattcaat ggaacgtgga cagccatttg
    21841 gtctccatcg aaatcggcat tataagcttc acatacaagt ggatgcaaac ggattgcgcg
    21901 tccttgtact aaaactggtt cgaatgcttg gataccaagt cggtgaagcg taggtgcccg
    21961 gttcagcaat actgggtgtt ccttgattac gtcttctaaa acatcccata cttcgtcttc
    22021 ttgacgttcg attttacgtt tcgcattttt gatgtttgaa gctagttcgc gttgaacaag
    22081 ctcacgcatc acgaatggtt tgaataattc aatggccatt tcttttggaa gtccacattg
    22141 gtacatcttc aagaaaggtc caacaacgat aacggaacga cctgagtagt cgacacgttt
    22201 acctagcaag ttttgacgga aacgaccttg tttccctttc aacatatgag aaagagattt
    22261 caatgggcgg ttacctggtc cagtaactgg acgtccgcga cgaccgttat cgatcaatgc
    22321 atctactgct tcttgcagca tacgtttttc attttgaacg atgattccag gtgcattcaa
    22381 gtctaacaaa cgtttcaaac ggttattacg gttgattaca cgacggtaaa gatcattcaa
    22441 gtcacttgtt gcaaaacgtc caccttctaa ttgaaccatt gggcgtaaat ctggtggaat
    22501 aacagggata acgtccataa ccatccagct tggttgattt cctgaagcgc ggaatgcttc
    22561 taaaatatcc aaacggcgga ttgcacgtgt acgtttttgt ccttgtgcag atttcaattc
    22621 ttctttcaat tcggctgctt ctgcatctag atcaacatta tcaagtaatt gtttaatcgc
    22681 ttctgcaccc atagcagctt ggaattcttg tccatattgt tcacgttttt cgcgatattc
    22741 gcgttctgtc aacaattgtt ttttctctaa attcgtattt cctggttcga tgactacata
    22801 tgaagcaaaa taaatgattt cttccaaagc gcgtgggctc atgtctaata caagacccat
    22861 acgtgaagga atccctttaa agtaccagat atgtgaaaca ggagctgcta attcaatatg
    22921 tcccatgcgt tcacggcgaa ctttcgaacg agtaacttcc actccgcatc gatcacaaac
    22981 gattccttta taacggatac gtttgtattt tccgcaggca cattcccagt cttttgtagg
    23041 accaaaaatc cgctcacaga acaacccttc gcgttcaggt tttaaggtac gatagttaat
    23101 cgtctcaggt ttttttactt caccgtaaga ccagcttctg attttttcgg gagaagccaa
    23161 acctatttgc atactttcga atttatttac atcgatcaaa aggggttccc tccatttcat
    23221 ttaatggcaa accgcaagac ggctgatctt cgaccgcctt gcagctcata gaccggatta
    23281 gtctaaatta tcttctgcgg tttcaaagtt ttggatgaca tcgtcttttt catcttccac
    23341 ttgttcagca gctttctttt ctaattcttt ggcagtttgc tgttcggcaa attttgtcaa
    23401 agcatcaaca gtgatcaaat cgtcgtcttc atcgtccatg tcgcgaagtt cgatctctgt
    23461 ttcttcgata tccaagacac gcatgtccag acctagtgat tgcaattctt tcaccaatac
    23521 gcggaatgat tccggtacac ctggttttgg aattggttca cccttgacga ttgcttcgta
    23581 tgttttcaca cgtccgacta cgtcatctga tttgtatgtc aagatttctt gtaaagtata
    23641 agccgcaccg taagcttcca gtgcccatac ttccatttca ccaaaacgtt gtccaccaaa
    23701 ttgagcttta cctcccaatg gttgttgtgt aaccaatgag taaggtccga ttgaacgtgc
    23761 atgcaatttg tcatcgacca tgtgagcaag tttgatcatg tacatcacac cgacagacac
    23821 acgtccatca aatggttcac ctgtacgtcc gtcgtacaga acagttttcg catcgctggc
    23881 cataccggct tcacgaactg tttcccatac gtcttcatca cttgcaccat caaatactgg
    23941 cgttgctacg tggataccta actgacgtgc agccataccc aagtgtaatt ccaatacttg
    24001 cccgatgttc atacgtgaag gcacccctaa aggattcagc atgatatcga ttggtgttcc
    24061 atcaggtagg aatggcatat cttcttccgg cataatacgg gatacaaccc ctttatttcc
    24121 gtgacgaccg gccattttat ccccttcatg gattttacgt ttttgaacga tataaacacg
    24181 aactaacatg tttacgcctg gtgacaattc atctccagct tcacgagtaa agattttcac
    24241 atcgtgaacg ataccgccgc cgccatgtgg tacacgtaat gatgtatcgc ggacttcacg
    24301 agctttttcg ccaaagatcg catgcaatag acgttcttct gcagataatt ctgttacccc
    24361 ttttggcgtg actttcccta caagcaaatc gccatcttgg acttctgcac caatacggat
    24421 gatacccatt tcgtctaaat cttttaatgc gtcttccccg acattaggga tttcgcgtgt
    24481 gatttcttca ggtcctaatt ttgtatcgcg ggcttctgat tcatattctt caatatggat
    24541 agaagtgtat acgtcatctt ttaccaaacg acggctcatg atgatcgcat cttcatagtt
    24601 gtacccttcc caagtcatga aaccaactaa aacgttttgg cctagagcca tttcgccttg
    24661 ttccatagat ggtccgtctg ccagagtatc ccctttttcg actttctcac ccaaatgaac
    24721 gattggacgt tggttgtagc ttgttccgga gtttgaacga cggaattttg ttacatcgta
    24781 tttatctaat gcaccgttgt cgcggcgtac gcggatttca gaagcatcaa cgtattcaac
    24841 gacaccgtca tgtttacata acaaagcagc tcctgagtca tgcgctgatt tgtattccat
    24901 accagtacca acccatggtg cttgaggatt gatcaacggc actgcttgac gctgcatgtt
    24961 ggcacccatc aaggcacggt tggagtcatc gttttccaag aaaggaatac atgctgtcgc
    25021 aacggcaact acttgtttag gtgatacgtc catgtagtct actttatcaa tagatacttc
    25081 taagttttca ctttgagcac gagccataac aacgtcttga gcaaatgttc catcttcatt
    25141 caatgggctg tttgcttggg ctacgatgta atggtcttcg atatcagctg tcaagtagtc
    25201 gatttggtca gtaacacgac ctgtttcacg gtctacacga cgataaggtg tttcgataaa
    25261 tccgaattta tttactttcg cataactgga caaactattg atcaacccga tgtttgggcc
    25321 ttcaggagtt tcgatcggac acatacggcc atagtgggag tagtgaacgt cacgaacttc
    25381 ataaccggcg cggtcacgag tcaatccgcc aggtcctaag gctgaaagac gacgtttgtg
    25441 tgtcaattcg tcaagagggt ttgtttgatc catgaactgt gacaactgag aagaaccaaa
    25501 gaattctttg atacttgcca caactggacg gatattgatc aattgttgag gtgtcaatgt
    25561 ttcagtatct tggatcgaca ttctttcacg aaccacacgt tccatacgtg ctagcccgat
    25621 acggaattgg ttttgtaaaa gttcaccgac tgaacggata cgacgatttc ctaagtggtc
    25681 gatatcatcg acattgccga taccttccat caagttcaag aaatagctca ttgaagcaat
    25741 gatgtcggct ggacgaaccg tttttacagc tgcttctgga tagccgtttc cgatcacgtt
    25801 tacttcacgt tcagggtctc ttggtgaaaa tactttgatg acttggacag tcattggatc
    25861 tgtgaccaca ccgtcttctg atggatagta agtcactgaa tttaggccat tatcaatgaa
    25921 aggagctagt gtttccatga cttggtgagt cagaactgtt cctttttcaa cgatgatctc
    25981 gcctgtttct ggatcaacta atgtttcagc taatgtcaga ttcaatagac gtgttttcaa
    26041 atctaatttt ttgtttactt tgtaacgacc aacgtttgct aagtcgtaac gttttggatc
    26101 gaagaaacga gcattcagca agttacgtga gctgtctgct gtttttggtt cgcctggacg
    26161 caaacgttcg taaacgtctt tcaaaccttc ttctgttctt gagtcgcttg cgtttttatg
    26221 caagtctttt tcgactgtat tgcgtaatgt ttcgctgtcg ccgaagattt caaagatcgt
    26281 atcatcggaa ccaaatccta acgcacgaac taatacggtt aaaggaatct tacgtgtacg
    26341 gtcgatacgt acataagaga tgtcttttgc atcagtttcc atttctagcc aagctccacg
    26401 gttagggata acagtagaac cgaatccttc ttttccgttt ttgtcgactt ttccatggaa
    26461 atagacacct ggtgaacgga ctaactgaga aacgattact cgctctgccc cgttgatgat
    26521 gaatgttcct tgttctgtca ttaatgggaa atcaccgaag aatacttctt gtgctttgat
    26581 ttcgccagtc tcgcggtttg tcaaacgcaa tgttacatgt aatggtgcag agtagtttgc
    26641 atcatgcgca cgagcttctg ctactgtata ctttggttct ttcaattcat aatctacaaa
    26701 ttccaaagat agattgccat tgaagtcatc gattggcaaa atgtcttcaa acatttctct
    26761 aagtccctca tctaagaacc attggtaaga gtctgtttga atttcgatca aatttggtaa
    26821 ttccaacact tcactgattc ttgcgaaact tctacgttcg cgatgttttc cgtattttac
    26881 tacgtgtcca gccaagctct tcacccctct aaaatgtcat tcaaaaagac tacccgatca
    26941 attgttacaa taatatttca aatattattt ttaggtaaca acagacattt tttgcggctt
    27001 ttaggcaaaa aaaaaccaaa aatagaacat tctcttgaaa ccttacttcg tgagcttcta
    27061 cttttgtttc cttatattaa agccgaaacg gacaatattt cgtttctgcc ttttcttcat
    27121 cagatagttt ttgcaacttc atatactaca cttattttct ataaaagtca acacttcctg
    27181 gcgtttcttc tgctttttcg caacattttc ttaatgtttt caattttatg ttttcaatta
    27241 tctgacaatt cttaattgac agaccttttt atctcggtta tactttagaa aattcatttt
    27301 gtggaggaac ttctatgcga accaacatca accaaatcgc accaacacaa ctgaataact
    27361 tttactttag ctactttaga taggtttgta ttcatcatga tggatacaaa cgttaaacga
    27421 cagtttgtat ctatgatggc taaagaactt tgtattgcag caaaaagcca cgtagacaaa
    27481 atgctaagtg gctgagcagt gaaaatacaa agtaaaagta tcaagcattc tactttttca
    27541 atttattttc cgacttttca atccatttgg acattttgtc tgaatggatt ttttgtttcc
    27601 caaaattaag gaggaaaaag aaatgaaaaa aaatataatc atcggattag gcgctgccct
    27661 gctactagct ttaggaggtt gcgcacaaaa agacaattcc gcagaggcat cagatggaca
    27721 aaaaacaatt ggcgtattgc aacttgtaca acataactcg ttagatgctg cctataaagg
    27781 attcaaagaa ggattagcag aagaagggta taaagaaggc gataatctaa caatcgatta
    27841 tcaaaatgcc caaaacaatc aagacaacct gaaaagtatg agtgagaaac tcgtcaaaga
    27901 agatcctgat ttgctacttg gaatcgccac tccagcagca caatctttat taaatgaaac
    27961 aaccactatc ccgatgactg tcacagcggt cacagacttg gaacaagccc atttagtcac
    28021 atccaatgaa aaacctggcg gcaatgtgac tggcacgaca gatatggtgc caatcgaaaa
    28081 acaaatcggc ttactgttaa atatcgtgcc agacgccaaa acaatcggca tcatgtataa
    28141 caacggtgaa gcaaactcta agatccaagc tgatttggca gaaaaagaat tgaaaaaagc
    28201 gggcgtcaaa gtcaaagtac tgacagcaaa ttccacaaat gatgtccaac aagtgacgaa
    28261 aagtctagct aaagatgtag atggaatcta tattcctact gataacacgt tcgcaagtgc
    28321 ggcaagtgtt gtcggtgaag tcgcaaaaga aacaaaaaca cctgtcgttg ctggcgcagt
    28381 tgatcaagtc aaagacggcg gtctagcaac agtaggaatc gactacgaaa gcttgggtaa
    28441 acaaacaggg atcatggcag caaaaatctt agacggcaag aatccaagtg aacttcctgt
    28501 cgaaacagcg aatgatttgg aactgtatgt caataaagac atggccaaag cattaggcat
    28561 caatccagac agcatcaaag aagctgaata aaacacagga ggattctcat gacaaatttg
    28621 ctctctcttt tcttatcatc tgcatcgcaa ggggtcttat gggccctact tgcgattggt
    28681 gtatacgtca ctttccgtat cttggacatt gccgacttga ctgctgaagg aagctttcct
    28741 ttaggtgcag ggatcgcggc agtaagtatt acaaatggct atcatccatt gacagcttgt
    28801 cttctaggct ttttaggggg tgcggcagca gggcttgttt ccggactgct tcacacaaaa
    28861 ttgaaaattc ccgcccttct tgcagggatc atcaccatga ctggtttata ttctgtcaca
    28921 tcccgtgtga tgggtgcgcc gaacatctct ttattgggtc agccaacgat ctttcattgg
    28981 gtcgagtcat tcggcataac aaaagacaac gctgtactga tcgtcggtct gctttttgct
    29041 ttgatcgttg tatgtttact tgttttgttc ttacgtacag aaacaggact tgctattcgt
    29101 gcaactggcg ataatctggc aatgagcgaa gcaaacggga tcaacacaga taatatgaag
    29161 atcatcggtt atatgatttc taatggatgc attgctcttt ctggctcctt gcttgcgcaa
    29221 aacaacggct ttgctgattt aaattctggt attggaacga tcgtaattgg acttgcttct
    29281 attatcatcg cagaagtttt gttacgtaac cagccactat ttctccgtct cttgactgtc
    29341 gttttaggtg cggtcattta tcgttttatt ttagcgttag tcttcgaact gaatgttgaa
    29401 ccaagtgatt cgaaacttgc ttctgctttc gtgctagtga tctgtctttc tttcccgcag
    29461 atccagcaaa aattccattt acctcaaaaa aagaaaggcg gtagtgtcaa atgaatgacg
    29521 tcttaacatt ggaaaatctc catcatacct ttgaaaaagg aacgatcaac gaaaatcatg
    29581 tcctgaaagg cattaatctt tccttagatc ccggcgactt cgtgacgatc atcggtggaa
    29641 atggggctgg taaatctact ttgctgaaca gcatcgccgg tacattttct gtcgatcagg
    29701 gtcaaatttt attgaatgga aaaaatatca cgaaaaaatc tgttgtcgaa cgttcgaaat
    29761 ccatcagtcg cgtattccaa gatcctaaac tagggacagc cgttcgttta acggtagaag
    29821 aaaatcttgc tcttgctatg aaacgaggaa aaaaacgcgg cttcttccgt ggtgtcaaac
    29881 cacaagaccg aacatttttc aaagaccagc tggttcggct aaatttgggg ttggaaaatc
    29941 gtttatcctc agaaatcggt ttgctgtctg gaggacaacg tcaagctatt acgttattga
    30001 tggccacatt acagcgtcca gaactgatct tattagatga acacactgca gcgcttgacc
    30061 ctgctacttc caaaacggtg atggaattga cagaaaagct gatcactgag cagaatctca
    30121 ctgcttttat ggtcactcat gacatggaag acgcgatccg ctatgggagt cgactgatca
    30181 tgctgcatca aggaaggatc gtcgtagata tccagcaaga ggaaaaacag cagctcacag
    30241 tgccccaatt attagcgttg ttcaaacaaa acagcggcac ggcattaaaa gacgatcaag
    30301 tacttttatc ttcataatca gaaagaggat gggacaaaaa atgaacgccc accctcttct
    30361 tttttctctt tccttctttc gtttttgctt tatgtatcaa ccgttgtttc ttcgaaaact
    30421 attttgttct ctccttcttc ctgatagaat ggatgggaaa ggagcttgat gccatgacaa
    30481 cgaaagacaa ggtacttcaa ttattgaaaa agacaccaga ttttctttcc ggagaaaagt
    30541 tggcacagca actggaagtt tcccggacaa gtatttggaa agccatcaaa gaattggaaa
    30601 aggcaggcta tcgttttgag cacgggccta cagggtatcg ttacctgccc tctgacgtac
    30661 tagatgcaaa agaaatccag caagagctac ggtcgtttat tcctgatttg aaagtacaaa
    30721 cgatgttgac ttctgaatct acgatgaaag atgccaaact ggcagcagct caaggagatt
    30781 ctgtttcgaa gttatttgtt gctgatacac aagaacaagc tcgtggacgt tttggacggc
    30841 cttttttcgc tgaacctgga cgaggaatct acatgagtct cttgctccat ccagacaaac
    30901 ggtttgatga attgcctcaa tatacgatca tcgcagccag tgcctgtgtg aaagccattg
    30961 aatctgtaac cggtcgttct gcttcgatca aatgggtcaa tgatatctac atggatggaa
    31021 aaaaagtctg tgggatttta tctgaagcag tcagtgatat ggaaagcggc aggatcagtc
    31081 atatcatcat tgggatcggg cttaattttt ctgttcctca aaatcatttt ccagctgatc
    31141 tccaagaaaa agccatctct gtctttcccg acggacaagc tagtgtcaca aggaatcagc
    31201 taatcagcaa aatctggcaa gaattgttca cttatctttc tcacccagaa aaagcgttgg
    31261 acttctataa agaaaaatca ttcgtattgg gaaaaaccgt gtcgttcaat caaaaaggaa
    31321 cgacttatgt cggtcttgca aaagaaatcg gtgatcatgg cgaactgatc gtccaaacaa
    31381 atgaacagaa gatgagtttg tcttctggcg aaatcagtct cacatctatc cgtacgcaat
    31441 aattatcaga tttcacaaaa ggacacctga tctcatacag tgtccttttt tctgaattct
    31501 cttcaaaaga ctctccttat tctatcagct gacagtttct ctatcctcta gaaaaaggta
    31561 ttctaaaaat atctataaag catggtacac tttctttaaa tgaatcaatg caaaaaacaa
    31621 gagagtcatc agtttacacg atcaaaagag atagcaggcc gcttcctttt tcaatcttat
    31681 agatagacca tgggtaacga taaaaacaaa aaagaacttg cgacttattt tgccgtaagt
    31741 tcttttttag ctagtgcctt aatctcttaa tcccaacaaa cgtctcgcat agatcacctg
    31801 accagagtgc atagcagcat catcaataat agaaacgaga cgcattcctc gtgtgactgg
    31861 tggtgtccag ttctcgtcaa ttacttcatc tagctcggaa ggttccagcc ttcttaagta
    31921 agaaatagca aaatcagcag ctcctcgcaa gtatcctagc agatcttcta tttgattagc
    31981 ttggatctgc tttgcttgtt ccagactatg cttccaatct tgttcgttat ctgctatcgt
    32041 catcggaaac cggctttccc agtcttgact atgccaaatt ggagaagttc ctgctaaatc
    32101 agcaatctgg aaatctaatt ctcgagcggt atgccaagcc agccaagtca ttgatttgat
    32161 ctgcggggct ctatcttcca ctggaaaacg attggcctcc tcaatagaga cgccttcaaa
    32221 caaacgttca aaccgatcgt atgcccgatc cacactctcg catagcatat ctaaataatc
    32281 catttatttt cctccttttt atttatccta cttacttttt cctaattcta ggatacctac
    32341 ttatatgtta ctacacaccc ttatttttta cactttaaca gtcaccatca tcaatagttt
    32401 ggagattgaa agaaaaagat agtgtcgaac tatcaattga acaagaagca acaatatgaa
    32461 cgtttcatta atattcttta aataaacaat gatttttcaa acgaacagtt taagactagg
    32521 agaaaaagag tataatagga aaatgtttga aaggaaagga aggggaaaaa tgaaaaaaag
    32581 ggcaagttta ctagtcctag ttgctttgtt gttcaatatc ttttcgtatg gtgtggccgt
    32641 ttcagcggaa acatacattc ctaaagaccc tgggaatatt tataatgatc taactggtaa
    32701 ctatggcttg ttaggaattg ctagtcaatt ccacgtattt gctaaaaata agacaactgt
    32761 gaatgcacat acagacggaa atatagcaac acatgaactt gatgcaaaaa ataattttgg
    32821 tacagagctt catgaaggtt tattgaacca agaaataaat tatatacaaa aaactgattc
    32881 tttcgctaat tcttcaggca tacctacagg tagcacaaga atgaataaat ttgtcgttgg
    32941 cagtaacgtg gatgtagcac ttcaagatgg tcatcctaca gtcaatggat ccaggcttga
    33001 tcacttaact ccagatgatt tttataaaga tcgtgctggg catgaatata ttgatttcga
    33061 gcaggaattt gctaaattga atgtagcagc aaatgatctt gcaacgatca ctccagctaa
    33121 aacatatact gctgcagatt ttccagacat gaacaatcgg acaatcgatt taactgattt
    33181 atcagatacc gggtttttat tggtaaatat cgatgcagaa gtgttaacga tgaatacgcc
    33241 acttcaaatc attaatccaa atgatcaagt agttgtattt aatgtcatca acagttcttc
    33301 tgcattgaac gtacagtcac caatcaaata caatgatcgt tcaaatcatg aaacagaaga
    33361 tttttcggat gcgaatatct cttggaattt tggaaatgag atgactgatt tgacaatcag
    33421 tgctcctttt caaggaacaa tccttgcgcc taatgctact atccgcatta ctcaaaacca
    33481 agacggaacg attataggaa caaatgtgat tttagatgca gctaccaatc gttgggatcc
    33541 taacgaaata tttatcagcg acaatggtac agatacgaca gacacaagcg acagtacaga
    33601 tacatctacc acaagtgata gctcagattc atcaacttca agcgacagta cagatactac
    33661 ctctaccaca agtgatagca cggatacatc agcttcaagc gacagtacag atactacctc
    33721 taccacaagt gatagcacgg acacatcagc ttcaagtgac agtacagata ctacctctac
    33781 cacaagtgac agcacggaca cgtcagcttc aagcgacagt acagatacta cctctaccac
    33841 aagtgatagc acggacacat cagcttcaag tgacagtaca gatactacct ctaccacaag
    33901 tgatagcacg gacacgtcag cttcaagcga cagtacggat acatctacca caacaagtga
    33961 tagcacggac acgtcagctt caagtgacag tacagatact acctctacca caagtgatag
    34021 ctcagattca tctaccacta caagtgatag cacggacacg tcagcttcaa gtgacagtac
    34081 agatacatcc tctaccacaa gtgatagctc agattcatca acttcaagcg atagtacaga
    34141 cacgtcgtct accacaagcg atacacgtca tgcaagtgtc tccttctcaa aaacaagttc
    34201 ggataacagg acgaacttta acttaactaa taaaacaagc aatgacaatg gaaacaaatc
    34261 aaaaagagca cttcctaaaa ctggaagtca atcgaataat tggataacac tcgcaggcgt
    34321 tatattactt gtaatcggtt taagaatcac ttttatgagt tataagaaat cgaaaaggta
    34381 aaaaaatatt caagataaaa aagagcaacg tcgctaacgg acgttgctct ttatttttgc
    34441 tttttatcat aaatacaaga actacaattc aatttgtcaa aaaacacgta gatttccttc
    34501 tgagaaattt atttacgtat cgaacctttc cctattcagt ctttgcgatc caaaatcagt
    34561 tgggtaaggg aaaagagatt ctcttttgtt cgaagagagg tggcaggtaa tttctttatt
    34621 tccttcaaag ccttttctgt gtaagaggcc gctaacttcc tagcttcttc cacgcctcct
    34681 gtatgttcaa cgattttgcg tacggcgtcg acatcttctt ctgtcattgc cgctttcttc
    34741 tctaaataag gtagtagctt tggtttgttt ttttgcaaag agtagatcaa cggtaaagaa
    34801 tagacccctt gacgcatgtc ttctagtaca ggtttgccga tttcctggct ctcttttgta
    34861 taatctaaaa tatcatcgat aatctgaaag gccatcccga tagaaagtcc gatatctcct
    34921 gctttttttg cgaagcgctc gcttgttcca ctttcaaaag cgccaacaga acaacttaat
    34981 tggaacagtt cagctgtctt acctgaaata ttttccaaat attcttttac agaaagttca
    35041 taattatagc gatcatccat ctgccctaat tcgccattca atactttttc catactgcga
    35101 gaattcaatt ggagactttt caaggaagtg gagtaatcag acaatagttt aaaacagcag
    35161 acaaataaat agtctcctgc atagacagaa acgacattac caaactgact acggatatta
    35221 ggctgaccac gtcgcagatc cgcttcatca acgatatcat catggatcag tgtggccgta
    35281 tggagcattt cgatagaagc tgccaaagcg atggctttgt ctcgatcttg ctctggtcca
    35341 aattgtgaga acaatagctg ataggcagga cgcaacagct ttccgccggc atggatcgtt
    35401 tctttgattg cttgttcgac aggtttgttt ttcaggcgga cagtttcttc catccgtttt
    35461 agtgtttctg tcaattcttt acttaaagca ggatagtttt cccacattgg atgtagcttc
    35521 ataaaaaaac tccttgtgtg acgattattt tttggtaaac attgggctcc atttttgttt
    35581 ggttccctgc tcataatctt tttggaatcg ttgaagtgtc tgcacatgtc gtaaaagata
    35641 attcgctgcg atcccgattg caatccctga taaaatccct aaaactgaaa ggactggcaa
    35701 gtacaacatg actgtccatg attgtgcaat gaaagaagcc atcaaaagct gtccgacatt
    35761 atgcatgaaa ccgccggccg cactaattcc gatgatactg acacgttttg gtcctaattg
    35821 cttgaccaat aacattccaa aataactaag caaagcacca ctcatactat aaaggaatgt
    35881 ggaaatcgtt ccgcctagca aagtagtcag gaatagtcgt agccaaagta gtaagaagct
    35941 gtctcgtttt ttcatggtaa aaatggcaac gatcgtgatt agattggcta agcctagttt
    36001 tgcaccaggc gcaaaagaaa aaggataagg gatcatattt tccaataaac caatgatcac
    36061 tccttgtgca acaagtagcg aaatataaat cattttttgt aatttactca tggtgtgtga
    36121 aggtctaata aatcagactg tcttcgtcac ctccatctgt tgaacggact tcgatcacta
    36181 atttatgtgg caaacagacg atggtttcgc catttttcgt tgcccagcct cgacgtacgc
    36241 agatctggtc atcgcaatct gcttctttta tccgtactcg atcgccatct atctcgatca
    36301 agttataatc tccgtgttca tctttgtaag tatatgtgtt gctctcttct ccagccatca
    36361 aagggaatgt tttgatttct gtgccatcca cacgcagtac cgcttctttt tctgctgttg
    36421 cggtgttttg ctgaagacca aaaatcatga ctggtaaaaa agaaagaaca gtcaaaagca
    36481 aaataataaa aacatcccaa ggtttgagca gacttttttc agcaaattct ttaaggctca
    36541 tttttgttct cctttttcca gattaactca cccaatttta gcataaaaac aagaggttgt
    36601 gacacctgtg tcttgctttg agtaataaga acgaagaata gaaaacagct ttccatgttt
    36661 tctaattctt tgggcttatt accgagaagc agcaggtgga acaccgttta taacaagagg
    36721 ttgtgacaga agtgggcaac ttcaagaaat aagaaggaat ttacgaaaat tacttttcaa
    36781 gtttttgtga aatttcggct tatttttcga agaagctact tctggaacac cgtttataac
    36841 aagaggttgt aacagaggtt gtgacacctg tgtcttgctc atggaggctt atctcgggca
    36901 tcttattcct ttaattttac ataaaaagga ggctgtgacg gtttgtctca gcccctttct
    36961 attgaaatct tgcattcaaa taacgttctt tatctattta tttcttgccg tactgggaac
    37021 ttggtctacc gtaccaccaa ttacctagtg tacgttgtag ccatggaatt acccagcgat
    37081 ccaacccaat cgcacgtccg gaaccgttca tcaatgcgaa agcaacaaaa atgaaccaaa
    37141 tgtttaccca gtaaaacatg cctgataagc agaacgtgat caccaataca atcgttgtcg
    37201 cactgcatag ccatgtgaat aatccagcaa tcaatgctaa tgccaaggca atttcaaaga
    37261 ataccatgaa tttttggaag aacattgcta catcattgtt tggcatgatc acttgcatga
    37321 tctttgtgaa ccagtctggt gacttctcaa tcacagccat tggttcttca ccgtatgcat
    37381 aactcaaacc aaaatgtgca gcttgagtcg ctgcttctcc ggcacttgcc cctgctccgc
    37441 tggcagctgt ggttgcatca ccagcagcag aagcaccact ggcaacatct gttacattgt
    37501 attcccatgt ccaagggaac acactgtttc cagtgaacca tgatccttca ccaaaccaag
    37561 tacttggttt gaacacttca ccattcccaa tgattttttt ggaagcttca acgaaccaaa
    37621 ctaaaccata gaaaatacga agtggcacac tccataagac attgccgtaa cgagaagaat
    37681 gtcctcgagt cacgttgcga tcatctttga tatggaaaaa ctcatgcatt aaatattgga
    37741 acatataata accagaacga atatcaaaga agtatttcaa attcacgata tgtttcatta
    37801 cgatagctaa aaatccgctt aaatggatct tatcaaatag gttagctacc ccccatttgg
    37861 caccaataga aaccataaat ccttgatagt ttcctttaaa tttatgcttt tctccgcctt
    37921 tgatcgaagc cacgatattc gctgcggctg tgtgtcctgt ttgttcagca gcttgaacga
    37981 tctgcggtgt cggtgttcct gggaattctt cataaaagac taaatcacca atgatataga
    38041 tgtttttatc ttcgtagcct tttgcctgca tatattcatt ggcgatcaac cgctgtccgc
    38101 gtgctgattc taatccaaag tcagcagcgt cagaagttcc tttgacacca gctgtccaaa
    38161 tcagtgtgtg agtcggaatg ttgcgtccgt ctttcaattt gatatgatca gcagccactt
    38221 ctacgattgg tgcattcaat aataattcca cgttgtgctt acgcaaatag cgttcagctt
    38281 ttgcagcatc atttcgatcc aacatattca agatcgtcgg cattgcctct acgaccatca
    38341 gtgtgatttc gtttggatct aatttataat ctttggctag gcgatttttc cagtcgatca
    38401 gttccccaat catttcaata ccggtaaatc cagaaccaca gacaacgaaa gtcagcatgg
    38461 ctttacgcag ttcatcatct ggttccaatg cagcttttgc tactgtcttc tcaatatgtt
    38521 cccgaatcct tacagaatct tcaaatgacc aaagggtgaa tccgtgtgct ttgacaccag
    38581 gtgtaccaaa gtcattcggc tctccgccca ttccaaggat cagctgatca aaaggatagg
    38641 aacctaattt ggtcttgacg atttttcgtt ctttatcgat accgattaca gtgtctgtga
    38701 caagttgtac attcttttga cgagcaaaca aatgctgcag atcgtattgg attgcttctg
    38761 gttctacacg tccgccagct acttcatgca actcggtcat catagtatgg tacgaatgac
    38821 gatcgatcag agtaattgct acatcttctt ttttcagttt ctttgccaag tattttgtag
    38881 ctgaaacacc ggcatatcct gctcctacaa cgacaatatt ttgctttgcc attgatcatc
    38941 cgctccttat agactatcta tataatgaat atatgataat tcttttagtt tgatacttat
    39001 tacacaatct ccatatatta tatagatgtt ctgttctttt gtctaactcc ttttggattt
    39061 ttttcaaatt ttaaaataaa gcgttttttc acgaaatttg ctgaaagaaa acgtttgaaa
    39121 tattacatgg gttatttatc aaaaatagtt gacttatagg ctaaaaagat tagaatgtat
    39181 aatgaatcac aaagaaaaat tgtggactag aaatggaatg gtggggtatt aaaatgaaga
    39241 aaaaacgttt tgtaactgga tttgccgtat tagcattttc agctctagtg ttaggcgctt
    39301 gcggcgcaga tgacaacgga agctcaaact cttcttctga atcatctaca gcacaatctt
    39361 cgactgcaaa aagcacaaca gaatcatctg caaaagttgt agctggcgga gatttgcaag
    39421 acggcacata taaattagaa gaaaaaaatt attcaaatgg ttaccgtgca gtctttgaaa
    39481 tggttgtaaa agacggcaaa atcactgaat caaaatatga taacgtcaat gaaaacggcg
    39541 aatcaaaaac aaaagatgca gaatacaaca aaaacatgga agcaaaatct ggcacaaacc
    39601 cagaaaaata cattcctgag ttgaatgacc aattcttaaa agcacaaagc gcaagcggtg
    39661 tggaagtcgt aactggagct actcactcat ctgaaagctt ccaaaactac gcacaacaat
    39721 tgatccaagc tgctcaagct ggtaacacag atacaatcga aatcgacaat ggtgctgatt
    39781 tgaaagacgg cacttacaag cttgaagaaa agaactactc taacggctac cgtgtgcaat
    39841 tcgaaatgac agttgctggc ggcaaagtaa ctgaatcaaa ctttgattat atcgacaaag
    39901 acggtaaatc aaaacaagat gatacagaat ataatgaaaa tatgaaagca aaaagcggta
    39961 cagaaccaaa aacttacatc cctactctga acgacgaatt cgtcaaagca atgggcgaag
    40021 aagatggctc acctgctaat gtagaagttg ttacaggtgc tacacacagc tcacattcat
    40081 tcatcatgta cgcacaacaa ttagtgaatg cagctgaaaa aggcgacact caaacaatcg
    40141 aagtagataa tatcgtaaca gaaaaataat cacataatca tactaataaa agttggaggg
    40201 tgggacgata gccagattag gcagtcgtct cacctttcaa aatgaagaga ggaaaaagat
    40261 gcggaaaaaa ctactatttt ccttaatcac actgggcatc tcttttatgg tgctaactgg
    40321 ctgcagcaac tcagcaaata aaacaaccac tgaaagttca gcagctacca agatattgaa
    40381 agatccatat tctgatgaac agttcttgtt agggacttac gttcgtatcc gtgtgtatga
    40441 tgaaggcaaa gaatcagcat tgaaacctgc cttcgatcga gtaaaagaac ttggtgataa
    40501 aatcacgatc aatcaaaaag gttctgagat cgatgaagtg aatgagcaag ctgggatcaa
    40561 accagtaaaa gtttctgatg acgtctacac gttagtcaag cgggcctacg aatatagcca
    40621 agattctcaa ggcggattcg atatggcgat tggtgcaatc acacaattat ggcgaatcgg
    40681 ttttgatgac gccagaaaac cttcccaaga agaaatcgat caggcattga aattagtgga
    40741 ttatcataag atcgaactaa atgacaaaga aaaaactgtt tatctaaaag aaaaaggaat
    40801 gatcatcgat ttaggtgcaa tcgccaaagg ttacattacg gacgaagtag tgaaagtctt
    40861 gaaaaaacaa ggagtgacca ctgccatcgt tgacttgggc ggtaatgtct atgtcttagg
    40921 ccacagccca agaggcgaaa accaagactg gacagtcggc atccaagatc cgaacatggc
    40981 tcgcggatct gttttaggtt ctattaaaga acggaacaaa acattagtta cctctggaat
    41041 ttacgagcgt tatttagaag tagatggcaa aaaataccat catttgtttg atccaaaaac
    41101 gggttatccg ttcgacaatg atatcgcaag tgtcacgatc ataacagata aatcaattga
    41161 tggtgacggg ttgtctacag cagtcttttc aatgggtgtg aaaaaaggac tggaatacgt
    41221 cgaaagcgaa ttgaataacg ggacggaagc gatcttcgtg acaaaagacg ataaagtata
    41281 tgtgaccgat ccaatcaaag acaccttcaa attatcggag gactcccatt acacgatggg
    41341 tgaccgttct gaactaaaat aaggagtacg aaccatgaca ctaaaaatct ttttagaagt
    41401 agttgaaata cgaacaaagg tggctagtgt atttcctttt gcggtagggg tattattctc
    41461 tatcgctttt tttcatgaga tacattggct aaacactttt ttatttttta ttggaatgct
    41521 agtttttgat ttagcgacta ccgctatcaa taattacatg gattatgaaa aggctcattc
    41581 tgaagtatat aagtatgaag aaaatgtcat tggccgcaga aatctatctc ctgtattgat
    41641 tcgaaacatg attttagcta tgattgcatt tgtttttgtt atcggcgtta ttctaacgat
    41701 caatacgggt tggcttttac ttttgatggg atttatctgt tgttttattg gagtattcta
    41761 tacattcgga ccgattcctt tatctcgaat gcctttaggg gaaattttca gtggggtgac
    41821 tatgggcctt ggaatttttg ccatggtcgt ttatgtcaat acattcggtt cagatgtcta
    41881 tgatctgaca gtcaactttt catctggaca atttgctttg gtaggaaatc tttgggctat
    41941 agcagcaatc gtattagctt ccttaccctt ggtgtttacg attgccaata tcatgttggc
    42001 taataatttg cgggatcttg accgtgatat tgaaaatcat cgctatacac tagtttttta
    42061 tatcggacgt cctgcgggaa cagtcttatt ccagctattg atgtatgctt gctatttcgt
    42121 gattttaatg gggttgttga gcggtatcta tgaatggccg atcttggtca ccttcttgac
    42181 cttgccaatt gtgaagaagc atttggatca attcaagcaa acgttacccc agcctatcag
    42241 tttcagctat tccatcaaaa atatggtctt attcaatgcc agttatgtat tggggctatt
    42301 actaacaatc attatgcaag ccatttaaca gcatgaagag gttgtgaaaa aactgccctg
    42361 catcaagtaa taagaacagc caaataaaaa tagctcctta tatttttcgt ttggttgggc
    42421 ttattaccgc agatgcaagc ttttgaacac cgtttatcag gaggttgtga aaaagtcgct
    42481 tagtttcgag tattaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt
    42541 ggcttaatac cgagaaactg gcttttgaac accgtttatc agaggttgtg aaaaagtcgc
    42601 ttagtttcga gtattaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt
    42661 tggcttaata ccgagaaact ggcttttgaa caccgtttat cagaggttgt aaaatcagtg
    42721 ttttgtcctg agtattagag aaacaaatgg aaaaatagtt cctcatattt tgccgttttt
    42781 tgagctaata ccgaaggaca gctgattgaa cactatttgt tcggaaaaag tgactaacta
    42841 tgacacaatt tttttcacag tccctttttt cacttgattt gattcgtacc agcgaacttg
    42901 acgagaactt tcttggtttg gtaactatat ggtattcttt taacatcctc gtttgcttct
    42961 tctaatttat ttatacaaag tcacactttt ttaaacatct gttcttcgat acatttctat
    43021 ttcttttaaa catcgatacc aatattttga acttaagagt gcataccaaa tggattcatt
    43081 tactcttcgt cagttacgaa gataatcaaa atctccaact tccctactta tttcgttttg
    43141 agaattatcg aagcaatctt acataaataa ctaaaaaaca aaaaaccaga aaatcagtat
    43201 actgattttc tggttttaca t
//
LOCUS       DACDEG010000016        36551 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.016, whole genome shotgun sequence.
ACCESSION   DACDEG010000016 DACDEG010000000
VERSION     DACDEG010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.016"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            24..695
                     /locus_tag="IV142_000920"
     CDS             24..695
                     /locus_tag="IV142_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8484595.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            754..1194
                     /locus_tag="IV142_000921"
     CDS             754..1194
                     /locus_tag="IV142_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484596.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1206..1874
                     /locus_tag="IV142_000922"
     CDS             1206..1874
                     /locus_tag="IV142_000922"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP8484597.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1938..2561
                     /locus_tag="IV142_000923"
     CDS             1938..2561
                     /locus_tag="IV142_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP8484598.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2617..3003
                     /locus_tag="IV142_000924"
     CDS             2617..3003
                     /locus_tag="IV142_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP8484599.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3216..4607
                     /locus_tag="IV142_000925"
     CDS             3216..4607
                     /locus_tag="IV142_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP8484600.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5218..5586
                     /locus_tag="IV142_000926"
     CDS             5218..5586
                     /locus_tag="IV142_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP8484601.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5766..6707
                     /locus_tag="IV142_000927"
     CDS             5766..6707
                     /locus_tag="IV142_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP8484602.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6776..7507
                     /locus_tag="IV142_000928"
     CDS             6776..7507
                     /locus_tag="IV142_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP8484603.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7651..9831
                     /locus_tag="IV142_000929"
     CDS             7651..9831
                     /locus_tag="IV142_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP8484604.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9851..10333
                     /locus_tag="IV142_000930"
     CDS             9851..10333
                     /locus_tag="IV142_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP8484605.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10349..11110
                     /locus_tag="IV142_000931"
     CDS             10349..11110
                     /locus_tag="IV142_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP8484606.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11241..12575
                     /locus_tag="IV142_000932"
     CDS             11241..12575
                     /locus_tag="IV142_000932"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP8484607.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12882..13367
                     /locus_tag="IV142_000933"
     CDS             12882..13367
                     /locus_tag="IV142_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP8484608.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13360..14043
                     /locus_tag="IV142_000934"
     CDS             13360..14043
                     /locus_tag="IV142_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP8484609.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14169..16814
                     /gene="polA"
                     /locus_tag="IV142_000935"
     CDS             14169..16814
                     /gene="polA"
                     /locus_tag="IV142_000935"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP8484610.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16859..17695
                     /gene="mutM"
                     /locus_tag="IV142_000936"
     CDS             16859..17695
                     /gene="mutM"
                     /locus_tag="IV142_000936"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP8484611.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17659..18288
                     /locus_tag="IV142_000937"
     CDS             17659..18288
                     /locus_tag="IV142_000937"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP8484612.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            18340..18672
                     /locus_tag="IV142_000938"
     CDS             18340..18672
                     /locus_tag="IV142_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP8484613.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18984..19475
                     /gene="nrdR"
                     /locus_tag="IV142_000939"
     CDS             18984..19475
                     /gene="nrdR"
                     /locus_tag="IV142_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP8484614.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19498..20871
                     /locus_tag="IV142_000940"
     CDS             19498..20871
                     /locus_tag="IV142_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP8484615.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20871..21797
                     /gene="dnaI"
                     /locus_tag="IV142_000941"
     CDS             20871..21797
                     /gene="dnaI"
                     /locus_tag="IV142_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP8484616.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21926..22366
                     /locus_tag="IV142_000942"
     CDS             21926..22366
                     /locus_tag="IV142_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP8484617.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22510..23211
                     /locus_tag="IV142_000943"
     CDS             22510..23211
                     /locus_tag="IV142_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP8484618.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            23208..24329
                     /locus_tag="IV142_000944"
     CDS             23208..24329
                     /locus_tag="IV142_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP8484619.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            24344..25576
                     /gene="pepT"
                     /locus_tag="IV142_000945"
     CDS             24344..25576
                     /gene="pepT"
                     /locus_tag="IV142_000945"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP8484620.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25708..26616
                     /locus_tag="IV142_000946"
     CDS             25708..26616
                     /locus_tag="IV142_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP8484621.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26651..26923
                     /locus_tag="IV142_000947"
     CDS             26651..26923
                     /locus_tag="IV142_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP8484622.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26934..27980
                     /locus_tag="IV142_000948"
     CDS             26934..27980
                     /locus_tag="IV142_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP8484623.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            28231..30840
                     /gene="clpB"
                     /locus_tag="IV142_000949"
     CDS             28231..30840
                     /gene="clpB"
                     /locus_tag="IV142_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP8484624.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(30991..31662)
                     /locus_tag="IV142_000950"
     CDS             complement(30991..31662)
                     /locus_tag="IV142_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484625.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(31655..32149)
                     /locus_tag="IV142_000951"
     CDS             complement(31655..32149)
                     /locus_tag="IV142_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP8484626.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(32169..33389)
                     /locus_tag="IV142_000952"
     CDS             complement(32169..33389)
                     /locus_tag="IV142_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8484627.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            33536..35371
                     /gene="lepA"
                     /locus_tag="IV142_000953"
     CDS             33536..35371
                     /gene="lepA"
                     /locus_tag="IV142_000953"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP8484628.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(35465..36547)
                     /locus_tag="IV142_000954"
     CDS             complement(35465..36547)
                     /locus_tag="IV142_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8484629.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT        12165 a         6464 c         7816 g        10106 t
ORIGIN      
        1 atgatgaaaa aagggggaga aaaatgaatt ttttagttgt tttaaaagat actctgattg
       61 agcgatctac atttctatat gttcaattgc aggaaaataa aacattgctg catttttcac
      121 cagagttcca tctagagggg ctaacattag gagaaatcta tcaagcagaa gggctgtcgg
      181 ctgttttgca cttttttaag gtagaattgg aattaccagt aaaagattat atcgagcttt
      241 ctcttgaatc ttgggatcga gcagtggttg aactgtttcc agaaggttta atgatcgata
      301 caaacgatgg gaagatccat ctgactgcag cagaactgca aaaacaaatg cgttatcaaa
      361 tagatgacga aaattctttt tctattttta gacgtcagca aaagattcta aaaagctttt
      421 taaaagtgat cagtaaaaaa cggaatctga tcaaaacgac acagctgttg aaaaaatatc
      481 caggtctgct tcacacgtct attcaatttc ctcaattgat tacgatgatc catcgtttta
      541 tacgtatgca acagctacca tctaagaagc tgtttcttcc attgacggac acatttcgag
      601 tagtcaacag agaagataaa aagaaagtga ttgtgattga ctttaccaga aatcggaatg
      661 tccttcacca agttgcaatg gaaaaggcca attaaggctt tttctttttg gatttttttg
      721 tcataataaa tattagatga tggaggagat aatatgatgc aaacatggtt agaaaggcta
      781 atacgatttc agcagaacta tcacaaactt atgcagggaa gatatgcgaa atttgatcag
      841 ttgaatcgcg gtctattgat cgctgcgctt gtctttagtt tgctaagtcg ttggttgccc
      901 tatagaatcg gacaattgtt gttcatcgtc tttttcggat ggatgatatt tcgtttccta
      961 tcaaaaaaaa tctaccccag actaaatgag aatcaacgtt atttgaaata tcttgataga
     1021 gtcaaagaaa aatatcatca gataaaaaac aaaaagcaag tcaaaatgga tcgaaaaagt
     1081 tatacttttt ttgagtgccc gaattgtcat caaaagcagc gtgcacctaa aggaaaagga
     1141 cgaatacgtg tgacatgcaa aacatgtggt ataaaattcg aaacgaatgt ttaagggaga
     1201 aaagaatgaa attagtatta ttacgtcatg gcgaaagcga agcaaatttt gaaaattact
     1261 ggacaggatg gctagatgtc gccttaacag aaaaaggaaa agaacaagca gcagttgccg
     1321 gtcaaaaaat gcttgaagcc ggacttcaat tcgatgccgt ttatacctct gttttgaaac
     1381 gtgcgattct taccggacaa gtcgcactag aagaaatggg tcagttatgg cttcctataa
     1441 taaaaacatg gcgtctaaat gaacggcatt atggggatct tgtgggtaaa aacaaagata
     1501 agatgaaaga aatatacgga aaagatcaag taaaaacatg gcgcagagga tatacggaag
     1561 ttccgccgtt aaccgcagaa aatagatttg accgcagata tgatcacttg gatccgcatt
     1621 tgatcccata cggagaaagt ctggaaatga cagtgaatcg gatcattccc ttatggcaag
     1681 atcatctagc accaaaacta aaagatcggc aagatttact agttgtgggt catggtaata
     1741 gcatccgagc actgacaaaa tatttagaag atgttcctga agatcaaatg gatacgatcg
     1801 atatcccaaa tgcacaacca atccagtata cattagcaga tgatttgaca attctggaca
     1861 aaaaaatact ttaaagaaca cctttatcta aaagtgagag atagaaaaaa gaagcaaagg
     1921 aatggaacaa aaggaacatg aaaaattata tatgggactt tgatggtaca ttgtttgata
     1981 cgtatcccgc aatggtagat ggtgcatggc aagcactaaa agattttggt atcagtatgg
     2041 acaaaaaaga aatctacttc aaaatgaaaa agtattctac tagttatttg atcaatgaat
     2101 caaacttgaa tgccagagag tttaatgaat tatttcaccg atatgaaaaa gaaagtacgg
     2161 aagtttcccg tccatttcca gaaacgaaac aagtactgga aatgctaaaa gacaacggcg
     2221 gtcgtcattt catcctgacc caccgtttga cagaatcaac atggggcctg ttaaaagaac
     2281 atcgattggc acatctgatt gaggaagtag tagggatcga tcaggacttt cctcgtaaac
     2341 ctgatccagc atctttaaat tatttgatcg atacttttca tttagaaaga acagacacga
     2401 tgatgatcgg cgatcgtcgt ttagatatcg aagcagggaa aaatgcagga gtcgctactt
     2461 gtctttacga catcgatcat tttctgggtg agatcccggc agattatgtg gtaggaaatc
     2521 tgaatgaaat ccttaccttg cagagcatca ttaaaaagta gtatcccctt tcaaaggaga
     2581 tatgttataa ttttcataaa ggagtgataa aattatatgg aaatcagaag acaaaaagaa
     2641 gttgtagaag catttcatta tgatatgaga ttgccgaacc aagaagtaga aacagatttg
     2701 aaagtaggct tctcgccttt aaaagaaacg atcgaaaact atccgaaaga acatagcatc
     2761 gtaggagcac gcttagagtt ccgtttagtg tttgatacat atgttttatc tggaagtgtg
     2821 agtcagatca atcatatcct caatcggaaa attgaaggtc aaaatgatgt cacacaagaa
     2881 gaagtggacg agctagtagc accgttattc gatatcgtac gccgaatgtc ttatgaggtg
     2941 acagagatcg ccttagatca gcctggggta aaattgaatt ttcaatcaga aaaacaagaa
     3001 tagacagtaa aaacatacct gcgatagatt aaagctatca caggtatgtt tttttattac
     3061 taattgtaag caaaaagaat gtcatttcga taaaaatcgt ctaaaatata aaagtcactg
     3121 aaaaaaattt tttcaggagg atggttgaag aatcattctc gggaaccttt acttccttcg
     3181 atggacttgt cattaataat atgaggagga gaaaaatgac aacactcatc acagttttag
     3241 tggttctagt ttttgcggca ttattatatg tgttctatcg tatgcaaaag cgccatacaa
     3301 aattttcaac acgcgtcttt acagcattag gagttggtat cgtctttggc ggtctgcttc
     3361 aattgatctt tggtgtagac gacaaagtcg ttactcaatc aatggaatgg atcgggattg
     3421 taggtcaagg atatgtatca ttcttgcaaa tgctagttat gcctttagtg tttatttcga
     3481 tcattggtgc attcacaaaa atgaaagttt ctgaaaagct agggaaaatt agtgcgactg
     3541 tattgacttc tttacttgga acaacagcta ttgcagcatt gatcggaatt ggttcaacaa
     3601 tgctgttcgg actccaagga gctaaattca cacaaggagc tgcagaaaca gcacgaatct
     3661 ctgaactggc aacacgtcag gaaacagttg aaaacttatc gattccacag cagattttat
     3721 cctttatccc taaaaacgtt tttgctgatt tagcaggtag tcgtgcaaca agtacgattg
     3781 gagtcgttat tttcgcggca tttgtcggaa tcgcttatct aggcgtgaaa agaaaagcgc
     3841 caaaagaagc agcattcttt tcagatttag tcgacagtct gtataaaatc attatgcgga
     3901 tcgtgacgct tgttttgcgt ttgacacctt atggagtatt tgctttgatg acaaacgttt
     3961 tggcaacaag tgattttgca gctatcgtca atttaggtaa atttgtcgtt gcttcttacg
     4021 tagcattgct tgctgttttg attgtccact cactgatttt gattggtgca aaagtcaatc
     4081 caatcaacta tttcaaaaaa gcctttccgg tattgagctt tgcgtttact tcacgttcaa
     4141 gtgctggagc tttgccattg aatatcgaga ctcagacaaa agctttagga gtagatgatg
     4201 caacagctaa tttctcagca agttttggtt tgtctattgg acaaaacggc tgtgctggcg
     4261 tttatccagc tatgttggca acgatcgtcg caccagcagc tggagtgaat gtctttagtt
     4321 tggaatttat tttgatgctt gtggcaattg taacaatcag ttcttttggt gtagctggag
     4381 tcggtggggg cgcaacattt gcttcactga tcgttttagg ggcaatgaac ttgccagtag
     4441 caatcgttgg gttagttatc tctgttgaac cattaatcga tatggcacgt acagcagtaa
     4501 acgtcagcgg tagcatggta gctggtatcg tgacaagtgc acgcatcaaa gatttagatc
     4561 gcgatgtcat caatgatgat tctaaagtga tcgatgtgaa tgcatagtca aaaagaggtt
     4621 gtgatagaag tggtcagctt taagaaataa gaagaagttt cctaaaaata gtttctcaaa
     4681 tttttgtgaa atttcggctt atttccgaga agctacttct gaaacaccgt gaataagagg
     4741 ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc aaacaaaaat agttcctcat
     4801 attttacgtt tggttgagct tattaccgca gatgcaagct tttgaacacc gtttaagaaa
     4861 gaggtcgtga gcctgccgtt tcactctgag tcacaaagag caatttcaaa aaatagcttc
     4921 tcatgtttgt tgaatattgt gtcttgtgac cgaggagttg tcaggagaac accgtttacc
     4981 agaggttgtg acttgttttt agtcacgacc tcttttttta tttattccat ataaatacac
     5041 attatttatt ccgaaaagtg aaagcgcaaa taacaaaaac aaccaactta tttattatta
     5101 aagaaaagcg tatttaagta ggacgattct gttgagagct ttcgtagatt ttcccatttt
     5161 cctatggtaa actgacaaag ttgagaatgt tagaacagat cgtttaggag gaaacagatg
     5221 tttacgaatg aaataaaaat catgctttat gtggacgatg tagaaaaaaa tgcaacgttt
     5281 tggagagcag ttggctttgc ggaaaaagat cgtcaagaaa tggatgggac gcttataatt
     5341 gaagtaggag ttgaggacag tcaaacctca tttctgttat atgatcgagc atttgttgag
     5401 tcccattcac ctggcacagc tactagtgcc ccatctttga tgttttacag taaagatatc
     5461 tttggactat ataaaaaaat gcagacgcaa tcagtcacat taggtgatat ggtacagctt
     5521 ggagaagaat atgtctttaa ctttgctgat cccgatggca actactttgc ggttacaggt
     5581 aaataataga aataaagact ttgtttttct gtgcgaaaca ggaaagcgaa gtctttttaa
     5641 ttcaaataat aatattatgt aaacaaaaaa ctttccatgc gtaattatac acaagcaaat
     5701 aaatcgtcaa atgcgaacgc cttctttata ctgtttagga acgaaaagaa ggaaggaaga
     5761 aatacatgga ttatcaagta ctgctttatt acaattatac aaaaatcgaa gaaccagagg
     5821 aatttgccaa aaatcattta gccttttgca aatctttgga tcttaaaggt cgtattctag
     5881 tagctgaaga aggaataaat ggtactgtgt ctggaaccgt tgaaaacacg actgcctata
     5941 tgaaagcaat gaaagctgat cctcgatttg ctgatacgat gttcaagatc gatccaagcg
     6001 aaggacatgc ctttaagaag atgtttgtaa gataccgtcc agagcttgta agtttaaagc
     6061 tagaagagga cattgatcca aaccaagtga caggtgccta tctttcaccg caagaattta
     6121 aagaagctat tttagatgaa cagacagtgg tgattgatgc acggaatgat tatgaatatg
     6181 acttgggaca ttttcgaggg gctgtacgcc cagatatacg cagttttcga gagcttccag
     6241 actggatccg cgagcacaaa gaagaattta tggaaaaacg ggtagtgacc tactgtactg
     6301 gtggcattcg ttgcgagaag ttttctggtt ggcttgtccg tgaaggattc aaagatgtcg
     6361 gacagctgca tggcggtatt gctacttatg gtaaggatcc tgaagtccaa ggagaattat
     6421 gggacggcaa gatgtatgtg tttgatgaac ggatcgctgt cgatatcaac caagtagata
     6481 aacaaatcgt cggtcgcgac tggtttgacg gaacaccatg tgaacgatat atcaattgta
     6541 gcaatcctga gtgcaatcgt cagattcttg cttccattga aaatgaagaa aaacatttag
     6601 gagcatgtag catagagtgt gcgaaacatc cgcgcaatcg ctatgttcaa gaccatggaa
     6661 tctctccaga agaagcagaa ctgcggttta cagctttaat taaatagaaa acaatagttt
     6721 tgattgtcga ccatttccaa tatgcgctat actagcgttg aggatggtga tccacatgaa
     6781 atcattgatt tatgctattg gggatgtcca tggcaaacat gagctttttg aaaaacttat
     6841 tcactattat gatccaaaga tccatcagct tgttttatta ggggatctca atgatcgagg
     6901 tcctcgtaca aaagaatgtt ttttattagg aaagcaatta gtcgaagaaa caggagccat
     6961 ttacctccgg ggaaaccatg aagaatattt tttgcaattt ctgcaatcac cggaggactg
     7021 gtatgcgcct tatatgagaa atggtggaaa agaaacgttt gaaagcctga ttcacccagg
     7081 ggcatcggag gaatactccc caactgaaat ctcgatgatg atccaatcga gatattcaga
     7141 actgattaaa tttttagtcc agcgcccctt gtattatgaa tgggaaaatt atctttttgt
     7201 ccatgccggt gtggatttga caaagaaaga ctggcgtaag acaagtctaa gagactttat
     7261 ttggataagg gaaccatttc atgaagggaa aaacaataca ggtaagacaa ttgtatttgg
     7321 acatacgatt acaccaatgc tccatggaga tatgcagaca acagacctct ggattcagga
     7381 tcataaaatc ggaatggacg gtggggctgt attcggaggg tcattgcatg gggttgtttt
     7441 tgacaaggaa ggtattcttc aagatattga atatcagaac aacgaaggac cttggcatgg
     7501 aatctgacta atggaaagaa tgaaggagct gtgactttgt tgcggctctt tttattttta
     7561 tagagaatat tgtttctttc agtagtttaa cccaggataa aaagtatgat agaataaaga
     7621 agcaattttt agcagaaaag aggcatttta atgacggaaa atttgaacca gacgatcatt
     7681 cagcttgtac aaaaagaact ttcagactat cgtcctaagc agttgactac tgtacttaat
     7741 ttgttaaatg aaggaaacac tgttcctttt atcgcgcgtt accgaaaaga aatgactgga
     7801 agtcttgatg aagtccagat tcgtgaaatc gaagagcggt atgcctatct tgaaaactta
     7861 gaaaaacgta aaactgaagt gattcggcta atcgatgaac aagggaaact gactcccgag
     7921 ttagaagcag aaatcaccca agccgtgaaa atgcaacaag tggaagattt gtatcgtcca
     7981 tacaaacaaa aacgacggac aaaagcaacg attgctaaag aaaaaggact tgaaccactc
     8041 gcgaagtggc tgatgcagct tacagatgga gaggtgcaat ctgaagcaga aaaatatatt
     8101 gacaaagaaa aagaagtctc ttctgccgaa gaagcgttgc acggtgctca tgagattatt
     8161 gctgaacaag tcagcgataa tgcaaaattt cggacttgga ttcggtctta tacgtataac
     8221 aaaggtatgt atgtcagcaa tgtcaaggat gagcaagcag atgaaaaagg cgtatatgaa
     8281 atgtattatg actttgcaga acccgtccat aaaatggttt ctcatcgaat attagctact
     8341 aatcgtggag aaaaagaaga tattctgaaa gtattctttc aagtagatga agcagcaatt
     8401 ttagcttact tggatcgcca aatagtcaaa aatcctgcct ctccttcttc ttcattcgtc
     8461 agagaagcct atcaggatag ttataaacgc tttattcagc cagcaatcga gcgagaactg
     8521 cgcaatgaat tgactgaaaa ggcagatgag caagcaatcg ctattttcgg cgaaaacctc
     8581 cgtaatcttt tgttgcaacc gccactaaaa ggtaaagttg tcctgggatt tgatccggct
     8641 tatcgtactg gatgtaaact agcagtcgta gatgcgacag ggaaagtttt agcaatcgaa
     8701 gttatttatc ctcataaacc tgcagcgcaa gcaaaaagag aagctgctgg accagctttt
     8761 atccaattaa ttaataaata tcaagtagat atggtagcta tcggaaatgg tacagccagt
     8821 cgggaatctg aactttttgt ggctgagcag ttgaaatccg ccgaccataa aacctattac
     8881 gctatcgtca acgaagcagg cgcttctgtt tactccgcta gcgaaattgc acgaaaagaa
     8941 tttccgcatc tacaagttga agaaagaagt gctgtttcta ttgcccgtcg cctgcaagat
     9001 cctttagctg aattagtcaa aatcgatcct aaagctgtcg gagtaggcca gtaccaacat
     9061 gacgtttctc aaaaacgttt ggctgaacaa ttggattttg ttgtggaaac agcggttaac
     9121 caagtaggtg tggatgtgaa tacagcaagc ccgcagctct tgcagcatat atcaggattg
     9181 aataaaacaa ctgcccaaaa cattgtcatt tatcgtgaag aaaacggaga attcaccgca
     9241 cgaactcaat tgaaaaaagt gccacgctta ggaccaaaag catatgaaca agcaatcgga
     9301 ttcttgcgag tccctggtgg gaaaaacatt ttggacaaca caggtatcca tcccgaaagt
     9361 tattctattg caaaagaaat attaacttca gtccaacttt cagaaaaaga gcttggtaca
     9421 gaagaagcag tagaaaaatt gacacgatta tccgtagaga aactggctga aagcctatca
     9481 gtcggagaag aaacgttagc cgatatttta gctgggctga cacaacctgg acgagatatg
     9541 cgtgatgaga tgccagcgcc cttattacgt acagatgtat tgagtatgga agacttgaaa
     9601 ccagggatgg aattgactgg aacggtccgt aatgtcattg acttcggcgc atttgttgat
     9661 atcggtgtga agcaagacgg acttgtccat atatcgaaac tcagcaaaaa attcgtcaag
     9721 catcctacag atgtcgtgtc agtaggagat atcgtaacag tttggataga acaagtcgat
     9781 acgaaaaaag gtagaatcag tttgacgatg ctttcccctt atgaagagta acaatgagga
     9841 gtaacaaaaa ttgacacaag aagaactgca gcagttagta gaagagatat cattaaaaag
     9901 cttccagctt cctttcaagc accaagcaat gttcaatcca cgcttacgta caactggcgg
     9961 gcgctatcat ttaaacgatc atcatttaga ttttaatgcg aagctatttg aagagtcaga
    10021 cgaagagacg attgcaggaa tcatcaaaca tgaactttgc cattatcatc ttcatctagc
    10081 tggaagaggg tatcagcata aagatatgga tttcaagtat cttttgaaaa aaacaggagg
    10141 gttgcgctat gcaccagcct tgacgaagaa acagataaag atggagtatt atcgatgcca
    10201 agattgttct gccacgatct gccgcaagag aagaatcgat gtacgcaaat atcgttgtgg
    10261 aagatgtcat ggagaaatca aatggattga gacaaaaaga gaagacttta atgaagcaca
    10321 aaaaatcaga tgaaaggata gaaggccttt ggaaaaaagt gagcagagga atctaaaaga
    10381 ctggaatcag ctattaaatt ttttagccgc acctgaagag ggaaattttg aggaactgac
    10441 ggcacctaca cttattttag ctggaaactg tcttcctatc ttagctgata aagcagcaca
    10501 aatgtattta aatggccaag tagatcaatt gtttttggtc ggaggggtgg ggcatgcgac
    10561 tagaatcctt tatgagaatt ttgaaaagca aggatttcat tttgaagatg gcatgagtga
    10621 aagtgaaatc tgtcgtcagt atctaaaaga agtctatgat ttgcccgata aggcttttct
    10681 gatagaatcg aagtctacaa attctggaga gaacgccact ttttcactgg agattcttca
    10741 ttccttagga gctgtaccag aaaaaatctt attgatgaat gatccgacac tgcaacgcag
    10801 gactcgagca acttttgaga aagtatggca aaatgaacaa actattttcg cgaatgctgt
    10861 gccatttgtg ccggaaatcc ttcacttttc tgaagagatc atctttacag caaaagaact
    10921 gaatcatcaa tggcctaagg aatatttcca tgcgctagta ttaggagaaa tggaacgtct
    10981 ccatgacgat gagaacggct atggtccgaa aggaaaagat tttattcctc acattgatat
    11041 atcagaggaa gtttggtctt cttacaccag aattaaagag tcaataaaaa cggatttttc
    11101 tcgtacttga ccattttagc aagcgaatcc tcctttccaa ataaatttgg ttgaaatttt
    11161 caatcgttta taagataatg aggagcgtgt tgaatgtaat agctaaaaga atggacgcta
    11221 gataagagaa gaggattcaa atggaaaatc aaaataaatt gtatcatttt gttggtatca
    11281 aaggttcagg aatgagctcc ttggctcttg ttttacatga acaaggtcta aatgtccaag
    11341 gatcagacat tgaaaaatat ttctttacac aaagagattt agaaaaagca aatatcacga
    11401 ttctgccatt taacgcagat aatgtaaaac cagggatgac gatcattgca ggaaatgcat
    11461 ttcctgattc acatgaagaa atccagcgag caaaagaatt aggtttggaa gtgattcgct
    11521 atcacgactt tattggtcat tttatccaaa attatacgag tatcgctgta acaggctctc
    11581 acggaaaaac aagtacgact ggactgctct ctcatgtatt gagtggtgtc cgtccaacaa
    11641 gttatctaat tggagatgga acaggacatg gtgatccgca agcggaattc ttttcatttg
    11701 aggcctgtga atatcgccgt catttccttg cctattcacc agactatgca atcatgacca
    11761 atatcgattt tgatcatccg gattattata caagtatcga cgatgtattt acagctttcc
    11821 aaacaatggc tggacaagtg aaaaaagcta tttttgcgta tggtgacgat gcttatttac
    11881 gcaaattaaa agcaaatgtg ccgatttatt attatggtgt cacagaaaac gatgatatcc
    11941 aagcacgaaa catcgaacgc acaacttctg gttctgcttt tgatgtctat catggggatg
    12001 aatttgttgg gcacttcact gtaccagcat tcggtaaaca caatatctta aatgctttag
    12061 gtgttattgc agtagcttat ttcgaaaaac tggatctgaa agaagttgca gaagaaatgc
    12121 tgactttccc aggagtaaaa cgtcgattca gtgaaaagat cgtagcagac atgaccgtag
    12181 tcgatgatta tgcgcatcac cctgcagaga tcaaagcaac gattgatggc gcacgccaaa
    12241 aatatccaga taaagaaata attgcagttt tccaacccca tacgtttacg cgtacgattg
    12301 cattgatgga tgaatttgca gaagcacttg atttagctga taaagtttac ttatgcgata
    12361 tttttggttc ggctcgggaa gaacaaggaa acgttaagat tgaagacctt ggagcgaaaa
    12421 tcaaaaaagg tggagaagtc atcaaagaaa ataatgtatc tccattgtta gattatcatg
    12481 atgcagtagt aatcttcatg ggtgctggag atgttcaaaa atttgaacaa gcgtatgaaa
    12541 aacttttaag cagcacgacg aaaaatgtac tgtaatacaa ttactgcagt gtccttataa
    12601 aaatagaaag agggggagtg gcaaaaatct tgccaagccc ccttttttgc aacttatgat
    12661 aaacggtgtt caaaagcttg catctgcggt aataagccca atcaaatcaa aaacatgaga
    12721 agctgttttt tcttggctgt tcttattatt tgatgcagga cagctttttc acgacctctg
    12781 taaactatag gaatctgtta aatcaaggga gcagaacttg ccttttacgc tacttttggt
    12841 acaataatgt atgattaaaa ataaactgga gggtttctat cttgaacata ggtaagccga
    12901 gaaaactagg aaaaacaata aatgaaatcg aggaaggcga ctcgttatcc ttaacagaat
    12961 ctattgaaga taaccagctt ttgctgtatt tgggattgac gaatgatgca aatcctttgt
    13021 atatccaaca tgactttgca caaaaaacag aatatcaaca gccaatcgtt ccatcagtga
    13081 tgctgatggg gatcatcaca agtgcaattt caaagcatct tcctggacca ggatcacatg
    13141 ttgtcaattt ttctgtcaat tttattgaac cggttttcca ttatgaaaca ctgacttttc
    13201 agtttgaggt cataaaagta gataagatga aggatgtggt gacgatttct gtagaagcgg
    13261 agaatacgaa cgaagatcgt gtgttggatg ctgtcgttat ggtccaaccg ccacaagttc
    13321 ctgaattgga aatagcaatc gaaagtgaag gtgaattgaa tgaataatcc aagtacatca
    13381 atgtcaggta tcaatcgttt ttatgcaaaa gtttatggat ttttaggtat gggacttgcc
    13441 cttagtgcag tcacttctta cttggctctt tgggtatttc ctttgcaatt agcaactttt
    13501 atcaataatt ttccactagg ctttgtgggt ctgtggatcg ttgaactgat tttagtaatg
    13561 gttctaggcg taaaagcaca aaagaatccg agcttagcaa tcggtggatt tattgcctac
    13621 tctcttttga atggcataac attagcggtt actcttgcat actacgatat tggaacagta
    13681 acgcaagctt tcttgacagc tactttgatg ttcatgggga tgtctgttgt cggagtaaca
    13741 acaaaaagag atcttagcgg ggttggaaga gcaggacagg cagctttaat cggagtgatc
    13801 attgcactag tcttgaacat cttcattttg agaagtagcg tagttgagct gttcatctcc
    13861 ctactgttag tcgtcatctt tgcagggatt acagcttatg ataaccaaag catccgtaat
    13921 atctatgtcc agacaaacgg aacagcacat agtggaattg ctgtattcat ggcattgaag
    13981 ctgtatttgg actttatcaa cttgttcttg gcgtttctga gaatctttgg acggaacaac
    14041 taaaaaagaa aaaaattcat cagttggggc tataccgaat tatttggtat ggtcccgctt
    14101 tttcttttcg cattccgcag gattctttgt tagaatgaat aagaacttta cagtaaggag
    14161 ttaagaagat gacaaaaaat aaattattac tcgttgatgg gaacagtgtg gcttttcgcg
    14221 cattctttgc gcttcacaat tctcttgaac gtttcaaaaa tagaaacggt cttcatacaa
    14281 atgcaatcta tgcattcaat acgatgtttg aaaatgttat gcaaaaagag caaccgactc
    14341 atgttctggt tgcgttcgat gcaggaaaaa cgacttttcg gacagaaatg tatgcggagt
    14401 acaaaggtgg aagatccaaa acacctggcg aattcaaaga acaaatgcca tatatacgtg
    14461 atttgttgac aggactaggg gtacagtatt acgaattgcc caattatgaa gcagatgata
    14521 ttatcggtac tttggcagaa aaagtagaca aagatcaatt tgatgtcgtc gtgctttcag
    14581 gtgatcggga tttgactcaa ttagctagta aagaagtaaa agtggatatc actgtaaaag
    14641 gtgtcagcga tatcgaaagc tatacaccag aacatgtagc ggaaaaatat gatgggttga
    14701 cgccaaaaca gattattgac atgaagggac tagccggaga tgcctctgac aatatccccg
    14761 gtgtgactaa aatcggtgaa aaaacagcta tcaagctatt gaaacaatat ggctcagtcg
    14821 aaggaattta tgaacatatc gatgaaatga agcaaagcaa aatgaaagaa aatctgataa
    14881 atgacaaaga gcaggctttc ttatcgaaaa aattggcgac catcaatact tcagcgcctg
    14941 tggatgtctc tatcgactca ttgaagtatg aagggaaaaa tctcgataaa ctcgttccgt
    15001 tttataaaga aatggatttc aatcagttcc tttctaaatt gaatatcgta gaagaagaag
    15061 taaaaatgga tgatattttg tttgaagtcg ttcatgaatt caaagaggaa atgtttacta
    15121 cggatatggc gttgtacgtg gaaatgatgg gcgacaatta tcatacagag gaaatcgtcg
    15181 gagtagcgtg ggggacagaa aagaaaattt atgtcacgaa cgatctatct gtattcgaaa
    15241 accgagcgtt ccattcttgg atcactgatc cgactcggct gaaaaaagta tatgatgcga
    15301 aacgaactta tgttgcttta aaccgctata ctggaaaaac gaaaggaatc gattttgatg
    15361 tcttgctagg tgcatacctt ctcgatacga atgaaaaaag cacagatatc gaaggaatag
    15421 ctgctcatta cggatacaac gatatccaat cagatgaagc tgtatacggc aaaggggcga
    15481 aaaaaggttt accagaagaa gaggaactct tttttgctca tttagcgcga aaagtagctg
    15541 caatcaatgc gttgacacca aaattgagcc aagaactggc agaaaaaaac caagaagatc
    15601 ttttccggaa aatggaactg ccattgtcta tcattttggc agagatggaa ataagcggga
    15661 tcaaagtaga tgcgactcgc ttacaagaaa tgaagggcga gttctctgct cgcttaagag
    15721 agatcgaaca aaaaatctac gaagaagccg gcgaagaatt caatttgaat tcaccaaagc
    15781 aattaggagt gatcttattt gaaaaaatgg gattacccgt tatcaaaaaa acaaaaactg
    15841 gatattccac agccgtagat gtactggaac agcttcgtga acaagcacca atcgtagagg
    15901 atatcctcac ctatcgtcaa attgcaaaaa ttcaatctac ctatgtcgag ggactgctga
    15961 aagtgatcca atcagatgga aaagtgcata cccgttatgt ccagacattg acccaaacag
    16021 gacgattaag ctctgttgat cctaatttgc agaacatccc gattcgatta gatgaaggga
    16081 gaaagatacg tcaggcattt gttcctagag aaaaagactg gctgatttat tcttctgact
    16141 attcacaaat cgagttacgc gtacttgcgc atatctcaaa tgatgaacat ttgaaagctg
    16201 ccttcctaga aggtcaagat atccatgcca gcacagcgat gcgtgtattt ggaatcgaaa
    16261 aggcagagga tgtcacacca aatatgcgta ggcaggcaaa agcagtgaat tttggtatcg
    16321 tgtatggcat ctctgattac ggactttcac aaaatctagg aatcagccga aaagcagcac
    16381 agcaatacat cgatacgtac tttgaaaaat acccaggtgt caaagaatat atggaaagaa
    16441 tcgtacgcga agcaaaagac caaggctacg tcgaaacgct gtatcaccgc cgtcgatatc
    16501 tgccagacat caattcaaga aattacaata tccgttcttt tgcggaacga acagctatca
    16561 atacaccaat ccaaggaagt gctgctgata ttttgaaaat cgcgatgatc gaattggata
    16621 aacgattaaa agaaacaggt cttcaagcta cgatgctgtt acaagttcat gatgaacttg
    16681 tcttcgaggt accggaaaaa gaacttgaat cattagataa attggtgaag gaagttatgg
    16741 aacaagctgt ttctcttcat gtaccactga tcactgacag cagttgggga aaaacgtggt
    16801 acgaagcaaa ataaacattg tttaagacag agcaatataa tgaaggtgaa aagaaaaaat
    16861 gccagaatta ccagaagtag aaaccgttcg taaaggattg gaacggttag tagtaggaaa
    16921 aacgattcaa aaagttcagg tcttgtggcc caagatcatc gagcaaccag agacacccat
    16981 ctttgaggct tctcttgttg gcgaaaccat ccaatccatt ggacgtagag ggaagttttt
    17041 aatttttcat ctcgatcatt gtgaactgat ctcacacttg cgaatggaag gaaaatatca
    17101 atttacgaaa gaaaatacac caatcgataa acatacgcat gtacttttct tttttgagga
    17161 tggaagccaa cttcgctaca acgatgtacg aaaatttggt cgtatgacaa tcgtcgaaaa
    17221 aggagcaagt gcaacttacc gaggaatcat gaaattggga cctgagcctt tgccagatag
    17281 cttcttatta gcagatttcg caaatggact gaaaaagtcc cacaaagcaa tcaagccatt
    17341 gctgctagac caacgtctag tgactggtct aggtaatatt tatgtagatg aagcattatg
    17401 ggaagcgaag atccatccag aacaaccggc gaacacactg cgccccaaag aggtagaaca
    17461 attacgtcta tcgatcattg atgttttgga tcgtgcaata gaagccggag gtaccacgat
    17521 tcgcagttat ttgaatgcat taggagaatc aggcggattt caagtagcac tgcatgtcta
    17581 tcagcaaact ggcaaaccat gtatacgttg tggaacgccg atcgtgaaaa caaaagtggc
    17641 acaaaggggg acgcactatt gtccgaattg tcaaaggcta aaaatcgcaa agtaggattt
    17701 gttttaggtt tgacgggtgg aatagctacc ggtaaaagta cggcagccaa agtctttcag
    17761 tctcatggat ttcctttgat cgatggcgat gtgattgcca gagaaactgt tgagcctgga
    17821 acgcttgctc tgaggaaaat tgtatcagtg tttggccaag aggtattgca gcctgatggg
    17881 caactagatc gagggaaact tgggatgatt gtttttccat caaaggaaaa acggcaaaaa
    17941 cttgatcagc tattagatcc gtttatccga aaagcgataa aagatcaaat cgcctctcta
    18001 agttccaatc atcctttagt aattgtagat atcccattgc tctacgaagg tcattatgac
    18061 cactatatgg atgcagtagc tgtcgtttat actacgccag aaactcagct tcagcgtttg
    18121 atgaaacgta atcaactgac gctagaacaa gcgcagcaga gaattagtag tcaacttcca
    18181 atcgaagaaa aaaagcagcg tgcggatatt ttgttcgaca acaatggaac aaaagaaaaa
    18241 cttgtgggac aaatcgaaaa ttggcttttt gctaatcgct ttctttgagt atctgtgtca
    18301 attttgctat cctgagtata tagaaagtga gggatagcga tgggatcatt aaaaaatcaa
    18361 aaggataaag tcgtaggtaa cgcaaaagaa aaaatcggcg agatgctcaa taaagaaaat
    18421 ctaatcgatg aaggtcgtac gcaatcagaa acagcagaag aaaaagaaaa gcagtaccaa
    18481 gcaagaatcg atgaaataga agaccagcag gcagaagaag aagcgaagcg gcaagatttg
    18541 actcaagaac gaaaaaatcc gacacctgat cgtagtaaaa caccgctccc agaaagtgac
    18601 cacattcccg atgagcaaaa aatcgaggaa cagcgaatga aacactatac aggaatcgaa
    18661 gagaagttat aaaatagtaa aaaatcgaga tgtgacttga agcagatgag aaggtgttca
    18721 tctgcttcaa gttttttgtg ctagatgact aaatcagctt ttgtgtaagt tatttctcag
    18781 taatcaaggt gatcagtatt ggaaaattga aatttagtaa ggacagggga cacaattacg
    18841 catgaaagta gaaaaaattg ttcttatatc cgtgtttttt gggtaggaca gtcgcgtttg
    18901 tgatttgttt cgtgttataa tacaactagt tgtaaagaaa gacacatcct ttatataaaa
    18961 tgcgatgcaa gaggtgagac aaaatgagat gtcccagatg tcaacataat aattcacgag
    19021 taatcgacag ccgtcaagca gatgatggac gtgcgatccg tcgccgtcgc gaatgtgaga
    19081 attgttcgtt ccgattcact acatttgaac gaattgaagc tgctccgtta ttagtaatca
    19141 agaaaaacgg ggaacgcgaa gagttcaatc gcgataaaat tttaagagga ctcatccgtt
    19201 cagcagaaaa aagacctgta gctatggaac aaatggaaca aatcgtcaac catgtcgaaa
    19261 accgcgtacg cagtctcgga gaaaacgaag tatcgacgaa tctaattggt gaatatgtga
    19321 tggaagattt agttgattta gacgaaatcg cttatattcg ttttgcaagt gtttatcgcc
    19381 agttcaaaga tatgtctgtt ttcttaaaag agttgcaaga tatcgtggat aaagcaaaag
    19441 aaaataataa agagaataat aaagaaaaca attaagaaac aaaaaggagg catttccttg
    19501 gaaaatgctt ggaaagagct tcaaccaaaa aatatttacc aaggaagaaa agcggctccg
    19561 ctgagagaag acagcaagaa cgccttgctg tatctttatc agccactaat cggcggagaa
    19621 gccttgagta tctatttcac cctgttatca gaaatctctc aagaatctgg caaaggtcct
    19681 gaagggatgc atgcagatct gcttagtagt ctaggctgcg gtatcccgca attttatgat
    19741 gcaagaaaaa agctagaagg aatcggctta ctggatgttt attttaaaga agatagtgat
    19801 ctgggacctc gttttattta tgagctgtta gaaccactca ttcctaaagc atttttcaaa
    19861 gactctgttc tggcttttct tcttctggaa aaagttggtg aacgacgatt ccagaagctt
    19921 acccaattct ttgaacctaa aaggcataca gcagagggat atcaaaaaat cacaaaaaaa
    19981 tttttagatg tttatcgctt caatgaagca gtgtacgctg ctaatcaaga aaacatcgaa
    20041 tcgctgaata aatcttttga agaatctgaa tcagtagcat tacttgaagc agattcgaca
    20101 ttcgattggc agtttttgaa tgattggctg gcaaaaaaac atattgcaag tcttgatgat
    20161 ccaatcatca aacaagtcga catgtatcat cagctttacg gtttcgatga attgacactt
    20221 ggcgaactga tcagtcaatc ttatgacttt agtgaacaaa aagtatctga aaaagaactg
    20281 caaaaagcca ttttggcaat gcagagacgt ccggtgaagc aaacaaaaga aacagttgcg
    20341 gtttctgaaa acagtaatga accctcgtca aacgaacatt tgacaaacgc agcgcttcag
    20401 ctgataaaag aagcacagag cattccaccg atgaaatatc tggaagcaat caagtcagaa
    20461 aaaggcggat atgtttctaa acaagaaaac tggttactgc aagatcttgt ttctcaatca
    20521 ggtctttcca gtagcgtgat caatgttttg ctgaattatg tactagtgat caagaataat
    20581 gctagcttaa atgccagtta tgtaaataca gtggcaaatg aatgggcaca aaagaaaatc
    20641 gccacagcag aagaagcgat ccagcacatt cggcaaataa gtaatcaagc aaaacaatca
    20701 aaacaaaaga agcaaacaaa ttatcaaact ggcaagcgaa atgttcgacg ggaaaagctt
    20761 cctgattggg tcaaccagcc aaaagatgaa aaacagatca gcagcgagaa aaaagcagag
    20821 attgaccgcc gttttaaaga atacctggca aaaaaggagg gtgatatcta gtggaagacg
    20881 taggcaaaga attgaagagg atcatcagca aacgcaatta tcaagaaaag tatgctgaaa
    20941 tgatcgctga agtcttaaat gatgcggatg ttcaagcttt tctaagcgca caccaagaag
    21001 aattgacaca agcggatatt gaaaaaagtt acgctaaatt gtatgaattc gtgcaagaaa
    21061 agcgtaaata tcaaaaaaac gatccaacga tgatcgctcc cggctatgag ccgaagctga
    21121 cgttgaattt tcattatgtg gatgtcactt atgtaccaac ggaagcattg ttagcacggc
    21181 aaagacaaga agaaatcaga agccgagtaa aagcaatgga tatgccaaaa gatattcaat
    21241 cggcttcttt tgaaaacttt gaacgtacag atggacgagg atatgcgagc ttagaagccc
    21301 tagattttgt agagaaatat actgctgatc caaaagggta tcataaagga ctgtatctag
    21361 taggaagttt tggtattgga aaaacatatt tattaggagc aacagcgaga gaattagcga
    21421 ttgctggatt caccacgacg ctagtccatt ttccgacatt tgccgttgag atgaaacagg
    21481 caatcggaaa agatcaagtg gcagaaaagt tagatgcagt gaaacgttca cctattttga
    21541 tgatcgatga cattggtgca gatgcgatga gtagttggat acgagacgaa gtattcggtg
    21601 ttatccttca atatcggatg caggaacagt taccaacctt cttctcatcg aattttacaa
    21661 tggatgaatt ggaacagcat ttgtccgtca ctcaacgtgg agatgaagag ccgctaaaag
    21721 cgaaacggat aatggaacgc atacgttatt tgacaaaaga aatcgaaatg actggtcgca
    21781 accgacgcaa tggttgaaca gttgacaagt ccaactgaca tttgacctag tagtaaaagc
    21841 aggtcagcga atcattatat ggacagaaat gaccggagac aatcaccgca acaagtaatg
    21901 cagaaactta aacggaggat tttttatgag ggctattttt tctagtatat ctgtagttgg
    21961 tctatttttt cttttagcag gctgcggtag cgcagatagc aacacaggtt ctgttgattc
    22021 gattgatgcg ggtcaaacag taaaaacaag cagtacgaaa acggagagac aaagcatatt
    22081 tgtcggaacg attgaaagta tcactcaagt agaagcagaa gaagaaacgc ttcagttgaa
    22141 actcgtcgat gtcatggcca aagaagatcc agcgaatatg ggaacgtcat tcagcaatga
    22201 cggagtgatc ttaaatgcta ctgctgaaaa aataagcgtg gacataaatg aattgaaaat
    22261 cggtgacaaa gtccaattta cattagttga gatgccgatc atgacgatgt ctatcccacc
    22321 gcaagttcct ggagattcga ttattgatgt ttcattgaac aaataaccaa taacattttt
    22381 tcaaaaataa gaaaatgttg ttttatagaa cgaaaaggta tattttttaa ggagatatgg
    22441 gaattttttt attcctagtc tccttatttt atttggaaaa tgctataatt atctagaagg
    22501 agtgaagcta tgaaccattt agatttatct cagcgcttag ccgcagtggg tgactttgtc
    22561 cctgaatccg ctcgtttagc agacattgga tcagatcacg cttacttacc cgttgcattg
    22621 atattaagag gaaaaatcga atatgcagtt gctggtgaag tcgttcaagg tccttatgaa
    22681 tcagcacgta agcaagtcaa aaaagatgga ttagaagacc gtatcaccgt acgccttgca
    22741 aatggtctag atgctatcca gccacaagat ggtattacag ctgtcacgat tgcgggtatg
    22801 ggaggttcac tgatccgaga tatcctagaa gctggcaagt tgaatcaacg cttaagcgga
    22861 caagaacgtt tgatccttca gccaaacatc ggagagcgta cattacgatc ttggctgcaa
    22921 gaaaatcagt ataaaatcca atcagaaaaa atacttgaag aaaataaaaa aatatatgaa
    22981 atcattgtag ccgaaaaaga acaaaaacca gttacttaca atgaaatgga gctgatgttc
    23041 gggccatttc tgcttgaaga gaaaagtgag atcttcaaat caaaatggac aagagagctt
    23101 caacaaagaa aagctatccg tgaacagctc aaacaagcag ctaatcaaga ggcacgtcaa
    23161 aaacaaatcg agcaggaaat ccaaatgatt gaggaggttt taaagccatg acaatcagcg
    23221 gacgcacttt catccaaaag tttgaaagct attgtcctca gtggctagct gaagaaggag
    23281 acccagtcgg actgcatatc ggaacattgg ataagccaat ccaacgagta atgatgacat
    23341 tagacgttcg tccagaagtg gtcgaagaag caatcaagaa aaaaatcgat ttgctgattg
    23401 ctaaacatcc tccaatcttt cgtcctgtaa aacggctggt tactgatcag ccacaagaaa
    23461 aaatgtatgc agatttgttg aaacatgata ttgccgtcta tgcagcacac acgaacatgg
    23521 atattatttg ggatggactg aatgactggt tttgtgaact tctcggtatt gaagtggaga
    23581 gttatttagt aaagactcat gagattcatt ataaaaaact ggccgtttat gtacctgtgg
    23641 atcatgcaca gaaaatgcgt gaagtattag ctgcagctgg tgctggtaca caaggtgatt
    23701 ataccggtac aagttttacc tcgattggac acggtcgatt tactccggaa gctggtgcac
    23761 aaccagcgat tgggaaagta gggaagacag aacaagttca agaggcaaaa gttgaggtta
    23821 ttttgccgga aactatcgaa aaacaagtga ttcaagccat gcggtcagct catccttatg
    23881 aggaacctgc ttatgatctt ttcgctatag atgaaccagt cgaaatgttt gggttaggac
    23941 gagtaggtga attgccgcaa gaaatttcta tagaggcatt tgtagaacaa gtaaaagaag
    24001 cgttccaact agatggactg agaatagtcc agccaaaaaa tgcaaaatcc tctgtgaagc
    24061 gcattgcgat ttgcggtgga agcggtgaaa aattttatcc gcaagcaatc gcccaaagag
    24121 cagatgtcta tatcacggga gatatctatt atcatacggc tcatgatatg caaagcgctg
    24181 ggttgattgc aattgatccc ggtcattata ttgaatcgtt atgcaagcaa cgatttatcg
    24241 agaaatttga atcttggaaa caagaagaaa attgggatat taactttttc gtttctgaaa
    24301 caaatacaaa cccatttcaa tttaattaaa aggagagaat actatgtacg aaaatctttt
    24361 gccacgtttt ttacgatatg tcaaaacaga aacacgctcc gatgcaacaa gcaaaacgac
    24421 tccatcgact cagacacaag ttgcttttgc gcaagaattg aaaaaagaat tagaagagtt
    24481 aggcttagct gatgttttct ataatgaaga taacggattc gttatgggaa cattaccaag
    24541 caatgttgat catgaagtgc gttccattgg atttatttcc cacatggata cagcagattt
    24601 taatgcagta ggagtcaatc cacaaatcat tgaaaactat gatggcgagt ctacgatcgt
    24661 actagatgcg gaagaacgct atacattgaa tacaaaagat tttccaaatt tgaaaaatta
    24721 tgaaggccac acattgatca caacagatgg cagtacatta ctgggagcag atgacaaatc
    24781 agggattgcg gaaatcatga ctgcaatgga gatcttgatc aataatcctt ctatccctca
    24841 cggtaccatc aaagtggctt ttggtcctga tgaagaaatc ggtgtcggag cggataaatt
    24901 cgatgttgag caattcgatg tagattttgc ttatacagtc gatggtggtc ctttaggtga
    24961 gttgcagtat gaaacgttta gtgctgctca agcaaatatt acgatccaag ggaaaaatgt
    25021 acatccaggg acggcaaaag atacaatgat caatgctcta cagttagcaa tcgattttca
    25081 taatcagctt ccagccgatg aagttccaga aaaaacggaa ggatacgaag gtttcttcca
    25141 tttgatggca ttggatggga atcctgaaga agcttctatg agttatatca ttcgcgatca
    25201 tgatcgtgaa aaattcgaag cacgcaaagc acaaatcaca catatccaag aagagttgaa
    25261 tcagcgattt gatcaagaac gaatcaaagt agatatgtat gatcaatatt acaacatgaa
    25321 agaaatcatt gaaaaagata tgagcatcgt ggaattagcg aaacaagcaa tgatcgagct
    25381 tggtatctct ccagtgatcg aaccagttcg tggaggaaca gatggttcaa aaatctctta
    25441 catgggtatt ccgacgccaa atctatttgc cggaggagaa aatatgcacg gacgattcga
    25501 atttgtttct ttacaagtaa tggaaaaagc aacagacgtg attgtaaaaa ttgcagaatt
    25561 gaacacaaag agttaaaatc gaaaaagaac agcttatgac aaggcgttcg tcccaatgat
    25621 ggaatgtcat ctgtctaagg ctaaagggca ggacaatgga attggttgtc ccgccctctt
    25681 ttttgaagag taaataggag gtaaagaatg gaaaagctgc tagtttttta taacaaaagt
    25741 tcagggaaag atgagggtga gaaattagca cgctggtttc aagattatgt aaaagaaaag
    25801 cagccagaat tatatgtaga actagctgaa actggcccat cgattgatga tgcggaatta
    25861 gtcaagaaag cagaagccat agaagctgat actttagtaa ttataggtgg agacggcacg
    25921 attcatcata tcgtccaagc gttccaaaaa acattatctc aataccatgt cggattactt
    25981 ccaggtggaa ctgtcaacaa ccttgcacgt gttcttgaga ttcccctcaa aaaagaagaa
    26041 gctgctgacg tgatcctgga aggcaatgta cggaaaatag actatggtca agtcaatgat
    26101 gaagtcatta tctctacact gacaatcgga atcttagcag atacagcagt ttggatcagt
    26161 caagaagaaa aacagcgtta cggctcgtgg atctttatca agaagttttt taagttattg
    26221 ctgaagaaga aaaaatatca tttggattta gaaacagacg aggagcattg gaaaggaaaa
    26281 tcccaactgt tgacagtcac gatgtctaac tccgtgggcg gttttacaaa ttttgacgac
    26341 tcagcgaccc tagatgatgg aaaatttcat gtaacgatcg taccttcctt agatattttc
    26401 cgatttattt tttatttgcc tagaatcatg agaggcaaat tctatacgat ccccggcatt
    26461 acttatttca cagccaaacg catgaaaatc caagcgcagg aaaaatctgt gggcactcga
    26521 acggatggag acacgacaga tgatcttcca gttgaattat ctgttgtatc aaaaggatta
    26581 acagtttatt caccaaagaa agaagaaaaa gcttagaaat agtagttaac agaaagttgg
    26641 aagaagagta atggatgtat tgttacacaa tcctttgtgg ttttaccttt tcattgtgaa
    26701 tttttactta ttttgtctga tggggtatga taaacatcag gctagaacta gcggctggcg
    26761 tgttcctgag aataatttga tatttgtcag cctgttaggc ggtggtttgg gaggattgat
    26821 ctctcgaaaa gttttccatc ataaaactcg gaaaaagaag tttactatag gattcgctgc
    26881 aggtgtactt gttgatttga ttctcattta ttttttccat taaggagcag gagatgtcgt
    26941 ctaaaggaac gaaaactaaa atcattctga atctgctttt gctgattgtg atttttgcca
    27001 ttatttatta tttaatcaat caatcatttg cagatatctt tgccgaattg ctgtctacca
    27061 gtgttgttgt tttggcagcg gctgttatat tagggacagt ttaccaaatc gctgaagggc
    27121 gctcaatcaa agaaattgct gaacctttcg cgccgaattt tacaacgaaa gatgggttct
    27181 tgacctctag ctatattgcg ttttaccgaa tcgtttcgtt tggtacagga acgctgatct
    27241 cggagattta tttttataac aaaaaaggaa tgaagctatc ccagggagca ggggtgacag
    27301 cactccatat gattatgtac aaactggcag tgatcactta tgcattagct ggcttggtca
    27361 tccaattctc tcttttttat tctaatggtc catcaatgat ctggtttatc atcgctggta
    27421 tccttttgac gatcgtgatt gttgcctttt tattggcgat gtccgtcagc ttgaatgttc
    27481 aaatattttt tgtcagctta tcgaataagt tatttaaaag tgagcgtttg cggaaatggg
    27541 tggatacatg caatcagcag atctactctt taagggaaac ggtccaaacc attatccaag
    27601 acaggactgc gctattgcgg atttatctat ggaatatgtt aaaacttgta ttttggtatg
    27661 tacttcctta tttgattctg attgaaaacc atcctaaaat agattttcta ctgaccattt
    27721 cgtttatcag ctttgcagta gtgctttcag gggttattcc tacgcctgct ggtattggtt
    27781 cctttgaatt tgtctatttg ttattattca gaccagttgt tggaacagtt gacgcagttt
    27841 cttcgctttt actgtatcgt ttcagttcct ttatcttgcc atttctctat ggatttgtgt
    27901 atgtgattgc tgaacggcga agagtgatca aagaagaaat aatagaagta aaaaaagaga
    27961 ataaagaaaa tcaagggtag gtcttttata agatctaccc tttttatcaa atcaaagaaa
    28021 aacgttatgg ataagataat tttacgtttg tttggctctt tttttatata catacaatca
    28081 attttgtttt tttaagattt atataagtcg agaaaaaaag tcaaaaaact atgtgtatat
    28141 tttgaccaaa attgaccaaa gcgatataat agtttttgta aagatcaaag aaataagtga
    28201 tcttaccttt aattaggagg gtatacctgt atgaatattg agaaaatgac tacgacgctt
    28261 caagaagcga ttgctgaagc tcaacagatt gcggtcactc gaaaacatca agatatcgac
    28321 attgcccatg tatggaaaat ctttttacaa tctaatcatt ttggtcgaaa tttctatacg
    28381 gatgccggat tggatgtcga atcttttgaa catgaaattg atcgtctact ggatgagtat
    28441 ccagttgttt ctggtggaaa cgtccaatat ggacaaaacc tcagccagaa tttctttcga
    28501 ttattgaatg aagcagacca aatacgtgaa agcttcggcg acgagtttct ttcaacggaa
    28561 gtcgttattt tagcgttgat gaaattaaaa aattatccgt taactgttta tttgaataaa
    28621 aacggattat ctgaaaaaga attgcgtaaa aacatcgaag agatgagagg aggagaaaga
    28681 gtgacctcac aaaaccaaga agaacaatac aaagcattag aaaaatacgg cgttgattta
    28741 gtacagcaag tcaaaagcgg aaaaatggat ccaatcatcg gtcgtgatga agaaatccgt
    28801 gatgtcatcc gtattctttc acgaaaaaca aaaaataatc ctgttctgat aggagaaccc
    28861 ggtgtaggga aaacggcaat cgttgaaggg cttgctcaac gtatcgtacg caaagatgtg
    28921 cctgaaaatt taaaagataa aacgattttt tcattagata tgggtgcatt gatcgctgga
    28981 gcaaaattcc gtggtgaatt tgaagaaaga ctaaaagcag tcttgaaaga agtcaaaaaa
    29041 agtgatggac gtatcctctt gttcatcgat gaaatccaca atatcgtagg ggcaggaaaa
    29101 acagaaggaa gtatggatgc cggaaatctg ttgaaaccaa tgcttgctcg cggtgaatta
    29161 catttgatcg gtgccacaac tttagatgag taccgtcaat atatggaaaa agacaaagcg
    29221 ttggaacgtc gctttcaaaa ggtattagtc aaagaaccaa cagtggaaga tacgatcagt
    29281 attctgcgtg gattaaaaga acgttttgag atccaccatg gtgttaacat ccatgacaac
    29341 gcattagtag cagcagctac tttatctgac cgttatatta ctgatcggtt cttaccggac
    29401 aaagcaatcg atttgatcga tgaagccagt gcaacgattc gggtagaaat gaactctatg
    29461 ccaacggaat tagatcaagt tactagacgt ttgatgcagt tggaaatcga agaagctgca
    29521 ctgaaaaaag aatcagatga tgcaagtaaa aaacgcttga aaaatttgca agaagaatta
    29581 gctgaacttc gtgaagaagc aaatgcgatg aagatgcagt gggaaacaga aaaagaagaa
    29641 gtcaattctg tttcagcaaa acgtgctgaa atcgataaag caaaacacga attagaagat
    29701 gcagaaaata actatgattt agaacgcgct gctgtattgc gtcatggtac gatacctcaa
    29761 ttagaaaaag aattgaagga attagaagct aaagcaaaag acagcgagat taagatggtt
    29821 caagagtccg ttaccgaaaa tgagattgct caagtcgttg gcagattaac gggtatcccc
    29881 gtaacgaaac ttgtagaagg tgaacgagaa aaactaatta aattgaatga aacacttcat
    29941 aaacgagtga ttggtcaaga tgaagcagtt gatgcggtca gtgacgcggt aattcgttca
    30001 cgggctggat tgcaagaccc aaatcgtcct ctaggttctt tccttttcct aggaccaact
    30061 ggtgtcggaa aaaccgaatt agcgaaagcg ttagctgaga acttgtttga ctcagaagat
    30121 catatggtac ggatcgatat gagtgaatat atggaaaaac atgctgtgtc tcgtctagtc
    30181 ggagctcctc caggctatgt aggttacgaa gaaggtggtc aattaacgga agctgtcaga
    30241 cgaaatcctt atacaatcgt cttgttggat gaaatcgaaa aagcgcatcc cgatgtcttc
    30301 aatatcttgt tacaagtcct agatgacgga cgcttgactg attcaaaagg gcgagtagta
    30361 gatttcaaaa atacggttct gatcatgacc agcaatatcg gttcacagtt actgctggaa
    30421 ggcgtgactg ctgatggaac gattccagaa gcagtggctg aacaagtcaa cacgctatta
    30481 cgtggaaact tcaaaccaga attcttgaac cgaatcgacg acacgatttt atttacacca
    30541 ttgagcctag acaacgtgaa aggaatcgtt gacaaaatgg tggcgcaatt ggcacaacgt
    30601 ctggaacatc aagaaatcct gctgacaatc agtgatgaag ccaaaacgtg gattgctgaa
    30661 aatgcttatg aaccagcata tggtgcaaga ccattgaaac gatttatcac taaggaagta
    30721 gaaacaccac tagctaaaga aatcgttgca ggacatgtta tgcctaaatc aaaagtaacg
    30781 attactttac ttgatggaca acttcatttc aaaacagaag agctagaaga aatcgtctaa
    30841 tcttccattg agactataaa agaaaaaagg ggtctgacac aactagtgtc agacctcctt
    30901 ttttcaaata aacagtgttc cagaagtagt ttcttcgaaa aataagccga aatttcttga
    30961 agctgcccgc ttctgtcaca acctctttta ttaggtattt ttcacttttt ctaatttata
    31021 actttcaaag taacgctcat cttttttact ttgagaccat gcaaacaatc ccattaaggc
    31081 agcacctatc gtatttgtca gcaagtcgcc catcgtatcc atcaatgctg ctcgtccgac
    31141 aaacagtgtg ccatcagaag cagcaaaacg ttgaagattc atgcctaaga actggtcgca
    31201 taagaattcc cagaactccc aaaacacgcc gcaaagtcca gcaaacgcaa agccgaataa
    31261 gaggaacaac caaggactgg tttttgatat ctcagcatct ttcatcaagt aaccaatcaa
    31321 tccatagcca agagctgtca ggaccattgg actcaccgca tgtaagattt tgtcccaaaa
    31381 agagatgata ctgatcaaat gcatccctgt gcctaaaaag acagagatga acaagaaaaa
    31441 ccagtaaaac aaaacgattg ctgctggtat tttaagtttg aaaactttca aaatgacttg
    31501 tggcatgaaa atcaatgcaa tccctaataa agattccacg ataaatgtga ttccttgaga
    31561 agtcgaatat ttttgtgtga taaattcata aaaattatag cagacaacga atacaccaaa
    31621 agcaagtaaa gctatttgca cctttttgat ttctttaatc actgaaagtc cctccatcta
    31681 aaaatctttt tacttctgaa agcaactcaa tttctgaaga aaccactttt ccgttcaatt
    31741 tgatcaaacc tattgtatat aagttcaggt agtgaaattg attttcagct gtttcttgta
    31801 gtgcagcgat ttttttcggg tgggttccgc cttgctgtcg agtatcagta tataagccga
    31861 tcactgggat gcctttcgca taagccacac cgatttccga tgctaccccg gcatcgatcg
    31921 tcagcccatc tagtaaagca atcattaaat cactagccaa cactttttct gtgtcagcaa
    31981 gagcaatcat tcgactgtct gcataagcag ttttgtcatt gattgcttca ttttcttgag
    32041 gaagatagat cgaaagctca gaagagagct ctctgatttc tttaactaac tgttggttat
    32101 ataacaaatc acttttagca aacattggtg cggcaaaata aattttcatc taaacatcat
    32161 cctttttatc aaaaataatc gctaaagtaa gcttccatgt ttggaatgat acgctctagt
    32221 gacttcagtg tttcttcatc tgtttctaac cgttcgattc ttgccaaata agaagcacgg
    32281 cgataattca tcatcaagca agtcaatgtt tggatatcca gtcgaacgga ttttcctata
    32341 ggttcattta agacacggac ttgtccctgt tcatcccatg ttaaagcgaa gacaccatta
    32401 ttccaaggag cgacaggatc ttcaatgatg aaatgaaaag catcagctgt tccaacaaac
    32461 ggaaaccgtt gaaggaattc ttttacatca acgatccgag ccataaaata tggttcgatc
    32521 tgctctttga tctgactatc ttcaatcaaa aatgctaaag gctcattttt ataaatatct
    32581 ccatgtaccc agtagatcat ggaaaaatgt gcggaaataa agttccataa gccatttcga
    32641 gcttcttgat caagatagaa catttctttt acgtgaaata tttcttcagc tacccagtag
    32701 aacagaacac ctcttggttc atggttgcta tcgtagtaga cagcggctgt gcgctcatct
    32761 tcattttcga aacgccaata ctcttcccaa tgaaaatcac tgcggaataa agcaccatgg
    32821 ttttgttggg caaatttgtc ataaacggaa aatacgtctg gatgatcaac tggtaatcgc
    32881 tcaaccatac cacttactgg gatttgttta ggtagctgtg tatctctgat tttaaaggaa
    32941 agtttatcag acatgatttc ccatccttta cgccgatagt aggggatact gtaaggatac
    33001 aaataagaga tccattgctt attcttgcgc atattatcca atgcaacgat aattagatct
    33061 tgcatcaatc catggttagc atattctgga taagttccaa ctccagtcac accacccatt
    33121 ttataacgtg caccgtgaat attgacttca caaggataga tagcgatttg agagatcagt
    33181 ttattttcgt ggaaccatcc gaacactttc gatacttcca aaatcggttt tttggaacgg
    33241 ataaaagctc gtttattctc aaacccgctt tcctcgatgt ctgcttcagt cacttggaac
    33301 acataggaaa gcaattcatt gaattgatcg atatgttctt gagtgaccgg ttttaattca
    33361 agattttctc taaaatcttt ttgatccatt ttttcatctc catttattag cttgataaca
    33421 gtatagcaaa attctacaaa aaaaacttct atagctgact aattaaaaac tctttaaatt
    33481 actgcttttc gttggtataa tggaaagtgc tgattatatg aaaagaggag acgctatgga
    33541 tataaagaaa atgaaagaac gccaagagca gattcgtaat ttttcgatca tcgctcatat
    33601 cgaccatggg aaatctacat tggccgatcg tattttagaa atgacgcaca ctgtgacgtc
    33661 tcgcgaaatg caagatcagc tgttagactc gatggatttg gaacgtgaac gtggaatcac
    33721 gattaaatta aatgctgtag aactcaatta cacagcgaaa aatggagaaa catatatctt
    33781 tcacttgatt gacaccccag ggcacgtcga cttcacctat gaagtctctc gaagccttgc
    33841 tgcctgtgag ggtgccgttt tagtggtcga tgcagcacaa ggaatcgaag cacaaacatt
    33901 ggccaatgtt tatttagcgt tagacaatga tttggagatc ctgccagtca tcaataaaat
    33961 cgacctaccc gcagcagatc ccgaacgtgt ccgtcaagaa atcgaagacg taatcggaat
    34021 cgatgctagt gaagctgtat tagccagtgc caaatcaggg attgggattg aagatattct
    34081 cgagcagatc gtggaatatg ttcctgcacc aagtggagac ttagatgcac cgctaaaagc
    34141 tttgatcttt gactctattt atgatagtta ccgcggtgtt gttctaaatg tccggatcac
    34201 ggacggtatg gtcaaacccg gagacaaaat caaactgatg agtaatggca aaacatttga
    34261 tgttacagag gtcggggtct tctcgccaaa agcagttgct agagactttt tgatggtcgg
    34321 tgatgtcggt tatatcacag caagtatcaa aactgtccaa gatacacgag taggggatac
    34381 agtaacgtta gcagacaatc ctgccgctga agctttacca ggttaccgaa aaatgaatcc
    34441 gatggtttat tgtggtcttt atccaattga tacttctcgt tacaacgatt tgcgggaagc
    34501 tttagaaaaa ctgcaattga atgatgctgc tttacaattc gaaccagaaa cctctcaagc
    34561 attaggtttt gggttccgct gtggattcct cggacttctt catatggatg tcgtccaaga
    34621 acgcttggaa cgggaattca atctagaatt gatcacgact gcaccatccg ttatttacca
    34681 tgttaataaa acggatggta cgactgctac agtagataac ccggcagatt ttcctgaacc
    34741 tgtaacgatt caggatgtag aagaaccatt cgtcaaagca cagatcatgg tcccaaatga
    34801 ttttgtcggt gcagtaatgg aactttctca acgaaaacgg ggagaattca ttacgatgga
    34861 ttatttagat gattaccgag taaatgtcgt ctataatatt ccgctttctg aaattgtctt
    34921 tgactttttc gacaaattga aatcaagcac gaaaggctat gcctcattgg attatgaaat
    34981 gtctggttat caaaaaagca agttagtgaa gatggacatc ttgctgaatg gagaaaaagt
    35041 cgatgcatta agctttatcg ttcaccgaga ctttgcctat gaacgtggaa aagcaatcgt
    35101 tgaaaaactg aaaaaactaa tcccacgcca acaatttgag gtgccaatcc aagcagctat
    35161 tggacaaaaa atcgttgccc gttcagacat caaagccttg cgtaaaaacg tcttggctaa
    35221 atgttatggt ggagatgttt ctcgtaaacg taaactctta gagaaacaaa aagaagggaa
    35281 gaagcggatg aaacaaatcg gctccgtcga agttccgcaa gaagccttca tggccgttct
    35341 gaaaatggac gaggacgaac cgaagaaata gcaagggttt gtttgaaaac agtgtaaaaa
    35401 ataaaccatg tttacatgga tttgccaccg gtttgccacc ggtggttttt ttcggtggca
    35461 aaaatcaaga attctttcta agattttcca tgatatcgac cgtttcattt ctcatcttat
    35521 ctgttacatg cgagtaagta tccatcgtaa ttgaaattct gctgtggccc aatcgttcag
    35581 agatttcttt catttttgcg ccattttcga gaagaagtgt agcatgagta tgtctgagag
    35641 aatggaagtt aaaagagagg gagagtgcat tcgatatcct cctagtattc catttcacta
    35701 cacttggcgt aactagctca ccgtcttcct tcgtacatac tgcatttgaa tcaatgtaga
    35761 gctttccata cttcattcga ttttctaatt gttgtttctt atgttttttc agaattgcta
    35821 gcaaggtttg tccaataaaa atcgttcgat tggaactgct tgtctttggc gtaccatata
    35881 cccatgcgcc gtcattcttt accatttgtt tctctacagt aattgttcca tttgaaaaat
    35941 cgacatcatc ccacgtcaga ccacaaactt cgccaacgcg cattcccgta taaaatccaa
    36001 tattcaaagg aatatagaaa ggatgtcctt caggagtaat ttctagcata tgatcaaagt
    36061 cctcaagaga aatgattttt agatcttttt tagtcgttgg tcgtgcttca tattttggta
    36121 tttttacata cagcatagga ttttgcttga ttaatcccca aggataaacc gccatattca
    36181 gcgcattctt aaggacagag tgagtaatag tcattgtttt cttcgagtaa ccctttttaa
    36241 attcagcatt gatgaaattt tgtaaaagag caggggagag atccgtaagt tttttctttc
    36301 ctaaataacc gtttatatga tttttgatgg taaatcggta gttttcatag gtattgtatt
    36361 ttagatttag tttaacgtat tcctccatcc aaaaatcaag gtattgtttt actcgagtat
    36421 ccgtacctaa aaagtattgt cctgtttcgt caatatctga taaaactttt cgtaaagcag
    36481 cttcggcctc tggtcgggtg tctccgccaa ctttctccac tttttttctt gagccatcat
    36541 cattgatatc t
//
LOCUS       DACDEG010000017        36167 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.017, whole genome shotgun sequence.
ACCESSION   DACDEG010000017 DACDEG010000000
VERSION     DACDEG010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.017"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(59..187)
                     /locus_tag="IV142_000955"
     CDS             complement(59..187)
                     /locus_tag="IV142_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP8484630.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="IV142_000956"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="IV142_000956"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP8484631.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="IV142_000957"
     CDS             complement(1674..2729)
                     /locus_tag="IV142_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP8484632.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="IV142_000958"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="IV142_000958"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP8484633.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="IV142_000959"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="IV142_000959"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP8484634.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="IV142_000960"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="IV142_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP8484635.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="IV142_000961"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="IV142_000961"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP8484636.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="IV142_000962"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="IV142_000962"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP8484637.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="IV142_000963"
     CDS             10491..12050
                     /locus_tag="IV142_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP8484638.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="IV142_000964"
     CDS             complement(12161..14092)
                     /locus_tag="IV142_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8484639.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="IV142_000965"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="IV142_000965"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP8484640.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="IV142_000966"
     CDS             complement(15021..15773)
                     /locus_tag="IV142_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8484641.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="IV142_000967"
     CDS             complement(15932..16141)
                     /locus_tag="IV142_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484642.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="IV142_000968"
     CDS             complement(16152..17480)
                     /locus_tag="IV142_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484643.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(17845..18432)
                     /locus_tag="IV142_000969"
     CDS             complement(17845..18432)
                     /locus_tag="IV142_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP8484644.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="IV142_000970"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="IV142_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP8484645.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /locus_tag="IV142_000971"
     CDS             complement(20012..21301)
                     /locus_tag="IV142_000971"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP8484646.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="IV142_000972"
     CDS             complement(21455..22387)
                     /locus_tag="IV142_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484647.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="IV142_000973"
     CDS             complement(22544..23203)
                     /locus_tag="IV142_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP8484648.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="IV142_000974"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="IV142_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP8484649.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="IV142_000975"
     CDS             complement(24244..24534)
                     /locus_tag="IV142_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484650.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24944..26749
                     /gene="glmS"
                     /locus_tag="IV142_000976"
     CDS             24944..26749
                     /gene="glmS"
                     /locus_tag="IV142_000976"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP8484651.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26821..28176)
                     /locus_tag="IV142_000977"
     CDS             complement(26821..28176)
                     /locus_tag="IV142_000977"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP8484652.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28200..29369)
                     /locus_tag="IV142_000978"
     CDS             complement(28200..29369)
                     /locus_tag="IV142_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484653.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29366..30253)
                     /locus_tag="IV142_000979"
     CDS             complement(29366..30253)
                     /locus_tag="IV142_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP8484654.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="IV142_000980"
     CDS             complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="IV142_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP8484655.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            34320..34841
                     /locus_tag="IV142_000981"
     CDS             34320..34841
                     /locus_tag="IV142_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP8484656.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(35540..36105)
                     /locus_tag="IV142_000982"
                     /pseudo
     CDS             complement(35540..36105)
                     /locus_tag="IV142_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         9805 a         7867 c         6429 g        12066 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcccgc ttttttgaag taagcgtcat aataaccagc acttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tctgagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 agttggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcttcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgcttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttctgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgacagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcaattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg tgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ttccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggga attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat atcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt tgccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccgatcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatatgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaaggtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtggtg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagagac tgggaaaaac cagcctcttc ttttttattt ctatgcttct gctgctgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgcca tgaggagcca
    12301 gtaatttgat cccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcagctc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcagagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccggcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagcaat tttttaccat acggaatgat ttcttctaca gattggaagg ttgtattgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaat ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagccggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cccaaaccgt taatttcagt ttcagtatct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg ggtccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattaaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctacgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctactc gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcaa aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgcga gcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt gaatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttaaaatc tttttataat ttaattataa
    17521 ccaacgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc tccctgaggt ggcacgcttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcacgttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttcaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttcattt aacacggaac
    21661 taagcagcgc atcttcatta ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgct actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcatg ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaatgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccta
    22621 gatgttgctt tttctgatta aagattgctg gaagataccc taacatcagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttga ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagatac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaagc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgcata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagac cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttgaagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt ctcttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgtcaatt catcttctac
    24361 cgtaaactca cgagcttcag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaagagcat ttgacaaagc atttgatata taccaattta
    24661 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    24721 cagataaaaa acaaaagagg aggatagtta taccttttag gatagcgcca ggtctgtgta
    24781 aacagatgac gaggagaggg tttatcgaaa gattcggcgg atggccctag ggactgcact
    24841 ctacagaaaa tccacaaaaa atatctgcaa agataaaaca aaaggatttt cctgcagctg
    24901 gaagcattca tacgaattca ttatagaaag taggatgttt tttatgtgcg gaattgttgg
    24961 aatgattgga ttaaaaaatg taacaccagg attgattgac ggtctagaaa aattagaata
    25021 ccgtggttat gattcagctg gtatctttgt ctcagatggc acaactgatt atctggtcaa
    25081 agcacaaggc agaattcaaa atttgaaaaa caaaataaat attgatacta caggaaatat
    25141 aggtattggt catacacgct gggcaactca cgggcaacct tctgaagaaa acgcccatcc
    25201 acatacttcc caaagtggac gcttcgtgct cgttcacaat ggcgtaatcg aaaactttga
    25261 agaacttaaa attgcttatt tagcaaatga ccactttatt ggtgagaccg atactgagat
    25321 tatcgctcat ctaattgaaa ctttcgcgaa ggatactaca acgcaagagg cgtttttaaa
    25381 agctttgcgg gtcatcaaag gttcttacgc tttcgctttg attgatcgga cagcacctga
    25441 tgttatttac gtcgctaaaa ataaaagtcc actattaatc ggactcggcg atggattcaa
    25501 tgtgattgcc agcgatgcaa tggctatgct tgctcatacg aaggaattcg ttgagattga
    25561 agatgaagag atggttaccg tcacatcaga aaaagttatt attcaaaatt ttgcaggtga
    25621 catcgtagaa cgttcttcat tcgaagcaca agtagattct tcagatatcg agaaaggtac
    25681 ctatcctttt tatatgctga aagaaatcga cgaacagcct attatcatgc gtcgtatcgc
    25741 tcaaaaatac gtaacagaag acaatcgtgt ctctcttgat caaaagctgg tcgatacatt
    25801 gtctgatagc gatcgaatct atatcgttgc ttgcggaact agttaccatg cggggttagc
    25861 aggtaaacag actctggaaa aattgacaca gatccctgtt gaggttcatt tagccagtga
    25921 atttggctat aacacacctc ttctttcaaa aaaaccattc tttattttct tgagtcaaag
    25981 tggcgaaaca gcagatagcc gccaagttct ggtaaaaatc aaccgtctcg gctacccttc
    26041 acttacgatt acaaatgttg ctggttctac tctttcgcgg gaagcatcct ttacactgct
    26101 attgcatgct ggacccgaaa tcgcagtagc atcaacgaaa gcttatactg cacaaatagc
    26161 tgtactaact ttattagcca aagctatcgg tgataaaaaa gaatttgaaa cttctcttgt
    26221 attcgatatc actcatgaat tgagtttagt tgctaatgcg atggaatcgg tcatcgccca
    26281 aaaagattat ttagaagagt tggcagaagt atatctcagc gatacaagaa atgcctttta
    26341 cattggacgt ggcgctgact acaacgtttc tcttgaagct gctttgaaat tgaaagaaat
    26401 cagttatatc caggcagaag gttttgccgc tggcgaattg aaacatggaa cgattgcttt
    26461 gatcgaagaa ggaacaccag tgattggaat catcagtgaa gaagtgactg gtgcgcatac
    26521 tcgaggaaac ctgaaagaag tcgaaagccg tggcgctaaa acactagtta ttgtctcaga
    26581 gggattagaa aaggaaagtg accagctggt gctaccaact gtccaccctt atctaactac
    26641 tctaaccaca gtcgtaccga ctcaattact tgcatattac gcaacgctat tacggggatt
    26701 cgacgttgac aaaccacgta atttagcgaa atcagtgaca gtagaataat aaaagaacaa
    26761 ggaccagtgt cagacatctg acactggtcc ttgttctttt attttttatg aaaatatctc
    26821 ttaatccagt ccgatttctt ctttgactac tgccgcgatt ttatcgacat agtaattaac
    26881 tttctcatca gtaggcgctt ctgccatcac tcgaagtaat ggttctgtac cggaaggacg
    26941 aactaggatt cttccttctg atcccatttc agcttctgcc tcttcaatca cttttttgat
    27001 tgccggaaca tccatcgcac cgtttttatc acttactcgg atattcacta atttttgcgg
    27061 atagattgtt acctctgctg caagctctga taatttctta cctgtctgtt tcatgatatt
    27121 cagtaactga attcctgaaa gcatcccatc tccagttgtg ttaaaatcta agaaaatcat
    27181 gtgcccagac tgctcgccgc caaagttata atcgtttttg cgcatttctt ctacaacgta
    27241 acgatcacca acttgtgtaa cgacatcttt caaaccgatt ccttccaccg ctttgtggaa
    27301 tcctaagtta ctcatgacag tcgtaacgat cgtatctttt ttcaatcgat ttttttctgc
    27361 caagtattta gcacagataa acatgatttt atcaccgtcg acaatgtttc caagttcatc
    27421 caccgcaata atacgatcgc catcgccatc gaaagctagt ccagcatctg ctcctttttc
    27481 aacgaccatc tcagctaaac gttctggatg ggtagagcct acgccatcat tgatatttaa
    27541 cccgtctgga tttgtgccca tggtataaaa atctgtttca agatcggcaa acaaacgatt
    27601 aacacttgtt gaagttgctc cgtttgctgc atcgacacaa atggtcaaac cggaaaggtc
    27661 accgttgatc gtttgtacaa ggaattgaga atatttcaat aagccttctg gaaattcttc
    27721 tacagttccc aatccttcag ctgatggacg aggcaattca tccacttcag catctaataa
    27781 tgcttcaatt tctagctctt ggtcatctac tagtttaaaa ccatctgcac cgaaaaattt
    27841 gattccatta tcttgtgctg gattatgaga agcagaaatc atgacccctg cgcttgcttt
    27901 ttgcaaacgt gtcagataag caacacctgg tgtagagatc acacctagct ggaacacttc
    27961 gatacccacc gataaaagtc ctgaaatcag cgcttcttct aacatttgac ctgagattct
    28021 tgtatcccgc cctactaaca cgcgtgggcg ttttccttct tcttcatgct ggcttaggac
    28081 ataacctccg caccgtccca gcttaaatgc aagttctggc gtaagttcct tgttggcttc
    28141 accgcgtacg ccgtctgttc caaaatattt acccatagtt gtaaaattac tcctttaact
    28201 taattttctg aggaatttgt tctgctcatt tgtgttgatt cgcttgtttc tgatgtttct
    28261 tctgtacttg tggtcgagct gcttgtccca gattccgaac tggaagaaat ggttgttgtc
    28321 gatggttccg ttcctgaatc agcagatgaa atacttctac tcgtacttgt attttcttgt
    28381 gtccgactgc tcgtcgaagc agagctactg cttgacgttt gcaacacggg tgtaatctcc
    28441 acactaactg attcgggctt gatacttaac ccctcttcta ctggtatttc aattttacga
    28501 gttgttcgat gattgatacc tgagacattg atcggtacag caatactgtc accgatcgat
    28561 gcaagcaaat cgctagagcc gcttacttct gcttccagtt ttgaaaaact gaaatcatag
    28621 ctttctaccc cggcagctgg tgttccttgc tgtatcccat aaagtcgtac cgacttgctt
    28681 ggcttgatcg aatcagctct cactgttact tgcactggat cagagacgat acttaatact
    28741 tcccctgctg cattcagcgc ctgtattggg acctcactat taatcccatc atctgtgacc
    28801 atcgaagcgt ccacatcagc tactactcgt ttgatttctg ccaatgtttt gtcacctgta
    28861 gtgatagaga cttttttagg actaacggaa gtatcatcaa gttcatatcc agatggtgtg
    28921 aacgttgaag gaatcaacgt ttccacatcg aaatctttta ttaccttttt ctcaaccgtt
    28981 actgtaatag tcgatggttc gatctttgct gtgacagcac tgctcaaatt ttgaattttt
    29041 agcggaacct catgtgttcc ttcgcccagc ttagtgatat ctgctgtgac gcggaacatc
    29101 cgtgtatctt cgtttgcctc tgcattcaac tgaactcggt ttgcactgga aagtttgaca
    29161 gtcactgcat tttcatatcc atggatatag tagcgctttg aatcataaga aatcttgatt
    29221 ggcaccccag taactgtttg attatacgct tcatttccag aaagcgtatt tggaacggac
    29281 tggccatttg cattgaagta cagcgcaata ctgaacagca atgcaataaa actgtaaaga
    29341 atgcttgtcc gtttctcttt tgagatcatt tgcgatcccc tcccttagtc ataccgtcta
    29401 ggaaagattg aatcacgttt ttcttgtttt ttgttttctc ttcagcaatc agttctctac
    29461 gcaaaattgc caaatattct tgctgggata agccagccat cagttcatta tctaatgtaa
    29521 tgctgacatc cccagtttct tcagagacaa cgatggtaat cgcgtcgctg acttcgctta
    29581 taccgacagc tgcccggtgt cgagtcccga attcttttgg aatcaacata ctttctgata
    29641 gaggaagata ggcgcttgct actgcgatct ttccttcttt tacaataact gcaccgtcat
    29701 gcagcggtgt attaggaata aagatattga tcagcagctc tccagtgata tctgcatcta
    29761 atgcaattcc tgtctcaata tactcatcta atccagtatg ccgttcgata gtaattagcg
    29821 caccgatttt tcgcttggac atatattgga tggctttgtc aaaagacaag atcatcttct
    29881 catcttcctg ctgttcgctt tttgattgtt tgaaaagcga actgcgtcca agatgttcca
    29941 gtcctctccg gatttccggc tgaaaaatca cgatagcagc tatcaccccg tacgtaatga
    30001 cttggttcat cagccaagac aaggtgtgca gaccaatgat ttccgctacg attcggataa
    30061 caataaaaat agctacacct ttcagaagct gaatcgcttt tgtgcctcgc agcagttgga
    30121 tcagtttgta tacaagatac cagacaacca gaatatcaag tatgttcacc aaaaaattca
    30181 gagagaacaa gtcaccggaa aacatttgct gccaatagtc caaattaaaa agttccccga
    30241 tttgaaatga cataaaaagc ctctcttctc ttttgagatt tcctctctag tatatcatat
    30301 gttttaattt tagaggctga tctgctcgta tttttcttga gaaaaacgca taattttcag
    30361 gaaaattgca agtatgcgtt ttatttttct ctgcaataaa tatttttaaa cttcagagaa
    30421 ttattcatta tattttttct gttatttttc ctttttttct tttgtagctt gttccatttt
    30481 tttacgtcgt tctgctctgg cagctcttct ttcttctgct tcaaggtccg tcgttttttc
    30541 ttttctagtc ttaacgggta catcgataga ttcctctggt tcaagtcgtt tcttgatttt
    30601 ttctaattct cccgtcagtg cagcatagtt gtaaaaacgt gttttagcgt cttggatagt
    30661 cttttcaaat aaagcttttg caagatttgg atttgcgttc tctagcgagg aaaagcgaac
    30721 ttgtttcctc atgaaatcag gcatagcaga gaaatcaggt tttttgtagt ccaaagtcat
    30781 tgggttcttg tttttttcga ccattgcagg gttatatcga tataatgacc aatagccaga
    30841 agaaacagct tcttttgctt ctttaagcga ttctttcatt ccaccaagca aaccatgtgt
    30901 gatacaaggc gtataggcaa tgattaaaga aggtccagga tacttttctg cttcttcaaa
    30961 agcctttatc gtctgcattt gattggcacc tgaagcaatt tgcgccacat aaacatttcc
    31021 atatgtcatc gccatcattc ctagatcttt ctttgaagca tactttccac tagccgaaaa
    31081 tttagcgatt gccgaagctg gtgttgcttt tgaggtttga ccgccagtat tggaatagac
    31141 ttcgttatcc aaaacaagaa tattcacgtc tgctccgctg gcaagtacat gatcgatccc
    31201 gccaaaacca atgtcgtaag cccatccgtc tccaccaatc atccattggc tgttttttac
    31261 gaatagatcc tttctcttgt atatatcctc cagttcaggg acaattgatc gttcgctttc
    31321 gatcgcagct tgcagttttg ctgctctttg ctgcgttcct tcgctctcaa acatgtgtac
    31381 caaccagtct tccatcaatt ctcgtagttc ttctgatact tttggcaata cttctttgat
    31441 tgaacttgcc aaagcctcgc gacgagtctg tgccgctaga agcataccat aaccaaattc
    31501 agcattatct tccaataaag aatttgacca tgcaggccct tgaccacatt cattggtcgt
    31561 ataaggagaa gcaggagaag caccgcccca aatagaagaa cagcctgtcg cattcgcgat
    31621 catcattcga tcaccgaaca tttgtgtcaa taattttaca taaggtgttt cgccacagcc
    31681 tgagcaagcg cccgagaatt caagaagcgg ttgattgaat tgtgtagaca agacagtatt
    31741 ctttcctttg actggatttg ctttttgtct taatgtcatt gcaaaagccc aattcacagc
    31801 ttgttctttc tgttcttcat aaggcttcat cactaaagct ttttcttttg cgggacaagc
    31861 ctctacacac agaccacaac ctgtacagtc ttccactgaa acttggatac ggtattttac
    31921 gccgtctgcg cctcgcattt ctctcacgat aaatccttct ggcgcttctt ccatttcctc
    31981 ttcatctgct aaaaatggac ggatggcagc atgaggacaa acaaaggcac attcattaca
    32041 catcgtacag cgatcactaa tccactcagg tacttctaaa gcgacgcccc gcttttccat
    32101 agaagccgtt cctaaaggca tttcgccagt tgtcattcca tttgctacaa gtgtaccaac
    32161 agagagttga tttccttcct gtgcattcac tggttcaagt atttcttgta tataaccagt
    32221 gttcctttta ttgatttttt caggcatctc caacgttctc caatggtctg ggatcgcgac
    32281 ttgatgaagc agttcggtcg tccgatcgat tgcttgtatg tttttttcta cgatagccat
    32341 tgatttatgt ccatagcttt tcagggcttc ttcttttagc agcggaagca cctcatcaaa
    32401 aggaatgatg tcagctaatt taaaaaaagc cgtttccatt gccgtattga tccggcgtcc
    32461 caaaccgact tcttgggcca atctcatggc attgatcgta tagaatctaa tgttattttc
    32521 agccaagtaa cgttttaatt ttaagggcag atgcgtttct aattgttcgt cgctccaaag
    32581 agtattcaat aaaaaaatgc cacctggctt caatcctttg accaagtcat agctatgcag
    32641 gtaagctggt gtgtgacagg caacaaggtc cgcatgctcg acaagatacg ctgaacgtat
    32701 cggcgtatct ccaaaacgaa gatgagagac agtcagaccg ccagattttt tcgaatcata
    32761 ataaaaatag ccttgcgcat atttatcggt gtgatctcca ataatcttga tagctgattt
    32821 atttgctcca actgttccgt cagatccaaa tccccaaaac ttggcctgga atgtttgcgg
    32881 ttctgtcaaa tccagacttt cctttggttc caaagattga taagtcacat catccacgat
    32941 ccctatggta aaccgttttt tgcgtatgct tgggtctttt ttcaattcat caaaaactgc
    33001 tgagatttgg tcgggagtca catctttaga ccccaatccg tagcgaccac caataacagc
    33061 cgggcgaagt tcagaatcat ataacgcact ttgaacgtct aataagagag gttctccacc
    33121 tgcccctggt tctttacttc gatcaagaac agcaattgtt tttactgtat gaggcaactt
    33181 ttgcacgaat gtttccactg gaaaaggacg ataaagatgg atattcaaaa aacctgtttt
    33241 ccgtccgttt gcattcaagt aatcaatcgt ttgttcaatc gtctgtgcaa ctgatcccat
    33301 agaaacaatg acttcttccg catcgtctgc tccgtaataa gtcaccaagt cataagaagt
    33361 tcctcgtaat cggttgatct ctttcatata tttttgaacg atttcaggta atttatcata
    33421 ataggggttg atcgtttccc gttgttgaaa atggatgtct ggattctggt tcgtaccact
    33481 aacagtcgga tgattgggat tcatccctcg tttacgaaat gaagctaatt tctcctgatt
    33541 gatcagagga gctaattctt catattccaa aacttcgatt ttttgaatct cgtgactcgt
    33601 acggaatcca tcaaagaaat tcaaaaaagg tacttctcct tcaatagaag caagatgggc
    33661 aacaggtgct aaatccatca cttcttgtac actgctttca gctaacatag caaatcctgt
    33721 ttgtcttgca gccatcacgt ctccgtgatc tccgaaaata ttcaacgcat ttgtcgtcac
    33781 tgcccgacta gcaacatgga aaacacttgg cagtagttcc ccagcaatct tgtacatatt
    33841 cggtatcatc aaaagcagcc cttgagaagc ggtatatgta gttgtcagtg caccagtttt
    33901 caatgatcca tggaccacac cagctgctcc tgcttcggat tgcatttcta ctacttttac
    33961 tggttgaccg aatacatttt cttttcctgc tgctgaccat tgatctacta attcagccat
    34021 ggtcgaactg ggtgttattg gatatattgc agcaatctcg gtaaatgcat aagaaatata
    34081 tgcggctgcc gtattgccat ccattgtttt catttttcgc atgattcatc atccttgttc
    34141 atattctctc ttattatctg ttccattata acgtaaaaca ttccattttt tcttttattt
    34201 ataaaaatat ttcacatatg tctatttgaa ttggtcatct aatttttaga caaacagtct
    34261 aaatcgttgc ctttttttgt cagacatgta taaactataa gcgaaagagg tgttttaaca
    34321 tggatatatc agtgattgat gcaacgaaaa caaatacaca aaaaggaatc ctttatggtt
    34381 catcgaatgc tcctaaaaaa atggtcgaat tcatcaacct atcttgtccg tattgccgac
    34441 aatggtttga agaatcttat gagctattag aagaagcagt ccaaagcggg caattgcagc
    34501 gagtgatcaa attgttcgat aaagaaaaag aaagcttgca gcgaggaaac gtgatgcacc
    34561 ggtatctcac aatcagcgat ggtcaaaaag cgatcaaaga gatcaaacaa atattcgata
    34621 ctcaagacga gtggaaacat ctttctctcc aagaagtagc ggattttgca gtagataaac
    34681 taaagctgac agaacaaaaa gacgaacagc tgagccaagc tgtcatcaac gaagctgagc
    34741 aagcacatat tcgatttgtt ccgactgtca tcttgggtaa agaaatcttt gatgaatcta
    34801 tttcaataga ggaattaaaa gaattgatcc aaacaaaata aaaaaataag gagcgtagga
    34861 caaaagtaaa gaacactttt gtctcacgct ctaaacacct ataaacggcg gaagcagaag
    34921 caactccttc ttatttcttg gagttaaacg cttctgtccc agcctcctta ttccgaataa
    34981 atggtgtcca aaagcttgca tctgccagat tatctcattt cttatgacaa gctgagcaaa
    35041 ttatctccac caaataattt attcatattt ttgacttgcg catactaact aattcttaaa
    35101 ttttcataca aaatttctca aattccctta acgagtaata tttatgtacg gtgattcaca
    35161 ctagtatccc aatcactttt taagttatcc cgatttcaat tctacttcaa aaatagttgc
    35221 ttttatgtat gctgttagtt gattagatgg tcgtttgttg agctaggtcg tttattttaa
    35281 acaagttgtt tctttagctt atacctatct cgtctaccaa acgcaagatg gcaaacacaa
    35341 atgcatacgc atctgtattt gccactttga ttcgtataaa gctaacaact cctaactcct
    35401 ctcatttaat agttgctctc ttttttattc agatacattt ttgctgatca cagcctagta
    35461 tttcaatggt tttgaacact aaaccaaatt gttccattac aacattcact ggctgatgaa
    35521 atggattaat agatttcgat tattcaaagc gattttctat tggtggattg tgttcccaac
    35581 taacaagacg gattccaaag tcattatata actcttcaag agttaaccct ttcctgttcc
    35641 caatagatgt atctctccca cgctcgatat aaaatatgga gtttcgaatg ccaagacctg
    35701 gcaaatcttt ccttgttaca gttacactgc tactaacttc cccatataaa atccagctcc
    35761 tccaaaatga taatatattt ttcgaccatc atctaactca tgtaacaata attgttgtgc
    35821 cctcttgtca ctcgccaaat gcaaacgcca cgtgcgatac catgccatgc tacctgcatc
    35881 tgcagaaaat ccatcaaata atgcaacaat tgtatcttct atcacaatcc tttccccatc
    35941 tacttcatat tctaatctaa aatcaaactc ccctcgggta atcgcaggtc ttggtggctt
    36001 aggacccaat attattctac ttaaaaccgc attcgttccc agatgaaaca cagctataac
    36061 aacaataatg attaatattt tgtttctatt tgataaattt ctcattattc gcaaatcacc
    36121 tctacattct cacatactag ttcgtaatac tcctttaata tcaaaat
//
LOCUS       DACDEG010000018        33197 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.018, whole genome shotgun sequence.
ACCESSION   DACDEG010000018 DACDEG010000000
VERSION     DACDEG010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.018"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(84..1058)
                     /locus_tag="IV142_000983"
     CDS             complement(84..1058)
                     /locus_tag="IV142_000983"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP8484657.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1138..2013)
                     /locus_tag="IV142_000984"
     CDS             complement(1138..2013)
                     /locus_tag="IV142_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP8484658.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2143..4050
                     /locus_tag="IV142_000985"
     CDS             2143..4050
                     /locus_tag="IV142_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP8484659.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4591..7869)
                     /locus_tag="IV142_000986"
     CDS             complement(4591..7869)
                     /locus_tag="IV142_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP8484660.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(8140..8685)
                     /locus_tag="IV142_000987"
     CDS             complement(8140..8685)
                     /locus_tag="IV142_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8484661.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(8697..9389)
                     /locus_tag="IV142_000988"
     CDS             complement(8697..9389)
                     /locus_tag="IV142_000988"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP8484662.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(9408..9692)
                     /locus_tag="IV142_000989"
     CDS             complement(9408..9692)
                     /locus_tag="IV142_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484663.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(9705..10268)
                     /locus_tag="IV142_000990"
     CDS             complement(9705..10268)
                     /locus_tag="IV142_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP8484664.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            10496..11158
                     /locus_tag="IV142_000991"
     CDS             10496..11158
                     /locus_tag="IV142_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP8484665.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            11282..12478
                     /locus_tag="IV142_000992"
     CDS             11282..12478
                     /locus_tag="IV142_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP8484666.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(12579..13955)
                     /locus_tag="IV142_000993"
     CDS             complement(12579..13955)
                     /locus_tag="IV142_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8484667.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(13992..14858)
                     /locus_tag="IV142_000994"
     CDS             complement(13992..14858)
                     /locus_tag="IV142_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP8484668.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(14947..15972)
                     /locus_tag="IV142_000995"
     CDS             complement(14947..15972)
                     /locus_tag="IV142_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8484669.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(15990..16664)
                     /locus_tag="IV142_000996"
     CDS             complement(15990..16664)
                     /locus_tag="IV142_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8484670.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(16848..17741)
                     /locus_tag="IV142_000997"
     CDS             complement(16848..17741)
                     /locus_tag="IV142_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8484671.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(17815..19512)
                     /locus_tag="IV142_000998"
     CDS             complement(17815..19512)
                     /locus_tag="IV142_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8484672.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(19505..20818)
                     /locus_tag="IV142_000999"
     CDS             complement(19505..20818)
                     /locus_tag="IV142_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8484673.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPQAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESSEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMDELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(20818..24855)
                     /locus_tag="IV142_001000"
     CDS             complement(20818..24855)
                     /locus_tag="IV142_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP8484674.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFYTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVYETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(25624..26142)
                     /locus_tag="IV142_001001"
     CDS             complement(25624..26142)
                     /locus_tag="IV142_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP8484675.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(26144..26761)
                     /locus_tag="IV142_001002"
     CDS             complement(26144..26761)
                     /locus_tag="IV142_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP8484676.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(26758..27612)
                     /locus_tag="IV142_001003"
     CDS             complement(26758..27612)
                     /locus_tag="IV142_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8484677.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(27609..28520)
                     /locus_tag="IV142_001004"
     CDS             complement(27609..28520)
                     /locus_tag="IV142_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8484678.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(28561..29895)
                     /locus_tag="IV142_001005"
     CDS             complement(28561..29895)
                     /locus_tag="IV142_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8484679.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTYTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            30103..30933
                     /locus_tag="IV142_001006"
     CDS             30103..30933
                     /locus_tag="IV142_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAP8484680.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(31177..31426)
                     /locus_tag="IV142_001007"
                     /pseudo
     CDS             complement(31177..31426)
                     /locus_tag="IV142_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(31617..31811)
                     /locus_tag="IV142_001008"
     CDS             complement(31617..31811)
                     /locus_tag="IV142_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484681.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSGF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(32316..32501)
                     /locus_tag="IV142_001009"
     CDS             complement(32316..32501)
                     /locus_tag="IV142_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484682.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
BASE COUNT         9730 a         7093 c         5815 g        10559 t
ORIGIN      
        1 aaaaaggagc tgtgacaaaa gtcttgtcgc agctccttga agctgctcac ttctgtcaca
       61 acttctttcc ctcttttttt tgtttattcg aattcttttt cttccagacg gaataacgga
      121 ctcatcggtg tattttcatg gatgcgtttg accgcttcac ccattaattc tgaacatgta
      181 acgattgtta agttttctgg atgacgattt tcttctgtga aaacagaatc agtgatacaa
      241 atatctttga ttggtgcatt gtctaaaata cttttcgcat gatcggacaa taatccatgt
      301 gacgcacagg catatacttc ttttgcgccg ttttctttta atacatttgc tgctcgtgca
      361 agcgttgctc ctgtgttcaa gatatcatct accatgatac atgtctttcc ttgaacatca
      421 ccgatcacat aaccaccctc agttgaagca tcatctgcat gatctacgat ggctaatgtg
      481 ctgttcaaat attctgaaag actacgtgca cgctgaacac cgctgttttt tggcgaaaca
      541 actacgatgt catctcccgt cagacctaat tgacggtaat aatgagcaaa caacggcatc
      601 gtaaataaat tatctacagg aatatcaaag aatccttgta cttgaaccgt gtgcagatct
      661 aaagtcagta ctcgagttgc ccctgcttct tcgatcaagt tagcgatcaa tttcgcagtg
      721 atcggttcat gaggtttcgc cgtgcggtct tgcctagcat acccatagta aggcaatacg
      781 acattgatcg ttttcgcact tgctcgcttc attgcatcga tcatgatcag tagttccata
      841 tagtaatcat tgacaggtgc attcgttgat tgaacgatat acacatgatc ccctcgaata
      901 ctttcttcga tattgatgga aatctcgcca tcactaaatt gcttcacgac gcttttacct
      961 aattctgttc cgaacacttt cgcgattttt tcagctaatg gacggttgcc attcaaacta
     1021 aaaatcttca acgtgtcatc ctgatactta ttactcatgt agtcctcccc aaaacttctc
     1081 tattcttcga tagtttaaca tatttttcca agattttctg ttaatttcag caaattctta
     1141 aggaagtgaa actgttccgt tactccaact ggcaggaatg actttatgat aatcgcccca
     1201 gttgtcgtgt actttgtaat aacgttctcc tgtttcagtt tcaaaataag cgtacgctgt
     1261 cacccaatga ttgccatatg tactgccaag tactttcaaa ataccaatca tcacaggttt
     1321 gccttcacgt atacgttttg tagctcgctg ccatgaacct agaagagttg ctcgtgcacg
     1381 ctcatgattg ccattcttct ttaaaaaact agaaatccct gtactaacct gaaaaggaat
     1441 cgtaggaagt cctagtggtt ggatctggct acgcaatgcc ttcgtcaaaa ccaacgaatc
     1501 agccgtattt ttcttgcgaa tctcatttgg aagcatccac cccttacgat agtcttgata
     1561 ataggcaagg agaactgctg ccgcgtaagt accacaaaga tagcctgagg gggtagccca
     1621 tttcttatac ctcaaaaaac gttcttctgg caatcccacc cagcaatttg cgagttcctt
     1681 tcctgacctt ttttcttcga tcaaaacggg aatatcttct tttctgttga tccatctaga
     1741 taactcgatt ggctcttcat tgatcagctt ccatttttta caagaaagac cctcttgata
     1801 agtatatttt gctggagaat attctaccgg atgtcctgat tgatcgataa tcacataact
     1861 ttgatcttca aaaaccagag ctaaaaaaag caatttactt tttcttgact gcagctgctg
     1921 agtgctggct aattttttaa tttttttatc tactttgaaa tataaaggtt tatccactac
     1981 ttgattcagt tgggatcgcc tccttttttt cattataagt gaaggattga aaaaaacaaa
     2041 tcagtttgat tgttcgaatt ttcaattttt tatgttctat ttgttaattc cttcattttg
     2101 taagataatg ggcacacgaa acaaaaaagg agcgatcgat gaatgaacaa agaacgattg
     2161 aaacaagatt tctatgaagc agtcaatgaa gaatggcaaa aaacagcaaa aatccctgca
     2221 gataaaccag ctacaggcgg tttccaagat ttagttgacg ggatcgacca acttttgatg
     2281 ggagaaacag acgatctgct tgcccatcca gaaaaagtgt cttcagaaaa aatgaaacat
     2341 ttccttgcat attatcaact ggcaaacgat tatgaccgaa gaaatgagtt gcatgctcaa
     2401 ccgcttcaac ctataatcga aaaagttgaa tcattgcagt ctttcgacga attgaataag
     2461 caatttccag aatggctgtt ggatacatta ccattgccat ttaatctgga tgtagatgca
     2521 gatatgaaga atgcgcaaac gaacgccttc tttgcttatc cgccttcttt attccttcca
     2581 gataagacgt attatgcatc agaccatccc aacggtgcac aactaatagg cgttttcttt
     2641 gatatggctg ttcagttatt agagcttgcc ggcaaagaaa aaagtcaggc agaagcaatc
     2701 acggaacaag cagtgcaatt tgataaattg attgctcctc atgtaaaaag tgcagaagaa
     2761 aacgcagatt acagcaaaat gtataatccg caatcttttg atacatttat taaatacaca
     2821 gataaactgg acttgaaaaa acttgtcacg ggtttgattg ggacaactcc agataaagtg
     2881 atcgtaactg atccagtgta cttcgatcat ttgaatgaca tcctgacaga agctcatttt
     2941 ccgttattca aaagctggat gatcattaaa acagtacgtt ctcttagtgg ttatctatca
     3001 gaagaattcc gccaagtcag cggaatctat tctcgcgtat tgtctggaac agacgaagca
     3061 atgcctcaga aaaaagcagc gtattacctt gcctctggac aattcgatca agtagttggc
     3121 gattactacg gaaaaaaata ttttggcgaa gctgctaaaa aagacgtaga acaaatggtg
     3181 aaaaaaatga tcgccgttta taaaaaacgt ctagaagaaa atgactggct tggggatgca
     3241 acaaaagaaa aagccgtcat aaaattaaat aacttaggta tccaagtagg ctaccccgat
     3301 aagatcccag cattattcga ccagttcaaa acagtacctg cagatcaggg tggtactttg
     3361 ctttcaaata ctcttgtttt cagcaaactc acattaaaag atcgtttcag caaatggaac
     3421 aaaccagtgg atcgttcaga atgggaaatg agtgctgaca cggtcaatgc ttattatcat
     3481 ccatttcgga atgtgatcgt ctttccggca gcgatccttc aagcaccatt ttacagtcta
     3541 gaacaatcaa gctctgctaa ttatggtgga atcggagctg ttatcgctca tgagatttcc
     3601 catgcatttg ataataacgg cgctttattt gacgaatatg gcaacttgaa caactggtgg
     3661 acagaagaag atcttacaca atttcaaaac aaagcacaag caatgatcga agaattcgat
     3721 ggtctgcctt ttgctgacgg aaaagtaaac ggaaaattaa ccgtttcgga aaatattgct
     3781 gatgcaggcg gtctaagctg tgcattggaa gcagcaaaaa cggaatcaga tctttctatc
     3841 gaggaattct ttgtcaattg ggcaacgatt tggcgtacaa aagcaaaaaa agaataccaa
     3901 caacttttac tgcaaatcga tgtccatgcc cctgctaaac tccgagcaaa cgtgcaacca
     3961 aaaaaccttg acgaattcta tgaaacattt ggtatcacgg aacaagacgc tatgtatcta
     4021 gcgccagaaa aaagagtgcg aatctggtaa gaggttgtgc cagaagtggt cagcttcaag
     4081 aaataagaag gaatttggga aaatcgcttc tcaaattttt gtgacatttc ggcttatttt
     4141 tcgaagaagt tacttctgga ataccatgta ttagaggttg tgaaaaagct gcttagtttc
     4201 aagtagtaag aaaaattttg tgaaaatggc tttccacatt tttgcataaa tttgacttac
     4261 taccgagaaa ctggcttttg aacaccgttt atcgagaggt tgtgaaatca gcgtcctgac
     4321 ctgagtatta gagaaaaaaa cggaaaaata gttcctcata ttttgccgtt tgttgagcta
     4381 ataccgaggg tcagctgatt gaacaccgtt tagtggagag attgtgaaaa agctgtcctg
     4441 catcaagtaa taagaacagc caatcaaaag cagcaaggag aacctcatct ataaaaagat
     4501 agaaggaagt gagacatcta ttggtctcat ttccttctat ttttactttc ctcagtcaat
     4561 tgttttacat cataaaagga gaaattccac ttattttttt cgctgtttca tgacataaac
     4621 aactacaccg attaacaaaa ttcctccagc taatgctagc aacgggttat taaattctcc
     4681 cgtttttggg agtccttgag tgccattttt ttcactttct tttgctgtcc tatccggatg
     4741 agaaggtttt ccatttggtt ttccatttgg tgattcattt cctggatttt ctctttctgg
     4801 tttgtcattt cccgggggca catcggcagt ttctcgcaca gtgatcgctt tttgctgggt
     4861 aacagattct gtatgagcag accatttttc cccatcaaat atttccgaac gataagtgac
     4921 ttctagcgta tattctcctt ttgaaggtgc tgaaaaaata gcggtatgat tagaaaattg
     4981 agcacttgac tgtgtttcaa cgatttggat cttttctggg aaataacgca tatcttcaaa
     5041 gacaggcgtt ccttgatttg gtgtgaaagt tgacacaaaa actgtaaaag gcttcagttc
     5101 aatgatattt ccttctgcat tctccgccac accatttgct tcttgtataa taatcggatt
     5161 attttgttca tttggttttg tctgatttgg ttcttgtacg attgcatact ctggatattc
     5221 cgaagacggt gaggtttctc cgtcaaaaat agcacctaca gataatggaa gaccatccac
     5281 tctttccttt tctgtttgac gattccccgg tttaatctgc atcagtccat gaacatggat
     5341 acttccattt tcatagcgcg taacaggctg ctgaccttct ttcgaacatg tatttagaga
     5401 ctcgatccaa tcttttgata tggtatgtcc aagagtattt tctgattgtt tctctcgttc
     5461 attccaatag tagttcaacg agtggtagat ttgatcttcg ttttgattca atgggcggat
     5521 ctgctctctc atctcaagat tctctgttcg gaaagataag ataccagaag catggtagtc
     5581 tgtgtttcct gctgaagagg tataaagagc cacattatat gaaccgttat taaaacttgt
     5641 actgctattg acgataatat ctgttccgct attggaatcg attcctttcg caagattttc
     5701 gaaagacaaa ctattgatca attcatgagg gcccgttaaa gaagaaccgc ccattttgaa
     5761 tccattaccg ttaccttctc cctcaattcc tggtacccag ccgtttcgat aagcaactga
     5821 gtttttgatg attacttttc cgatcgaacc tgtttcattt ttagcaaaaa ggtcccaacc
     5881 gtcatctgca ttatgatacg cgatacaacc gtcaaataca tttccttctc ctattgtcag
     5941 tttggccgca aatccatcgg catcttcaaa tcctggatcg gcattttcaa aagatgtaca
     6001 gttcttgact aagttatgcg cgggccatgc ttcgaaagga tcatttgagc taccgctgat
     6061 ttggattcct gtatttccat tccgataagc atgaatctct tcgatcaaat tatgatttcc
     6121 agaaatctgt aatccttttt gcatagctgc actatttgta atgtcaaacc ctcgtaaacc
     6181 ccagtagtga ctgaacagtg tcatcccatt tccagttcct ttaaaatcaa gtacaggacg
     6241 cttgttttct ggatttttct ctgcaatcaa ctgaatcggg ttttctcttg tcccgttaac
     6301 gccttttgga atcgtcaatc ctctttcgaa ggaataagtt ccaggcgcca tgacgattgt
     6361 ctgcccagca tatgcgtatc tcaatgcttg tgtcagagat aatggatttt gtctgcttcc
     6421 gtttcctgca tctgaccctt ctggtgttac ataaactgtc gaatatttag gtttacggta
     6481 gtccactgta tgaagaatct cttttgtttc ataactagtc atctctacgt attcttctgg
     6541 cggatagttt ttatctggcg taaaactgaa agtaaattcg ttcgttcctt ccatcagttt
     6601 tgtctgataa tcaaattccc gattcgcagt gatagccaag ttttttgcgt tcttaagcac
     6661 ctctcctgtt gctttatttt taattgtcaa taatccgtca gcatttgctc taaaggacat
     6721 agtatgatct tggtttcctg aatgattact agaggtcaca gtgtaaatcg gatcaatcaa
     6781 gcgagtaggt ctttcttgcc caggaggatc tgtcgcagga tctgagtaag caacttcgat
     6841 tccttctacc ttgatcgtca tattccttgc tgcagcaaat cctgcataaa taaccgattc
     6901 atctgaagcg tataattttt cccagtcata catgatgtca gaagccattt cttctcctgt
     6961 ttcaggatcg taataatatg cctctaaacc agtattcgtt tttttcatct ctacgtccag
     7021 tttagtaatc gagttcgtgg ccggaaacag attcccatga ccgtttccga atatattata
     7081 agaacctgtt tctttttcca agaaccctgc tgaagtctct aaaggtgttg tttccactga
     7141 gacactccca ggagctggcg cagcttgcgg atcttcagat gatattcctg tgattgcccg
     7201 tgtccccaat ccaagtcgca tcgtatattt gctgccgctc gtgttgacca cttcttgtct
     7261 atccgagtcc cagacatatt cgatccgact gcctagtatg gcaaatgaat ttgaataaaa
     7321 tgcctgccca aacaaatgat tttttggtac tgcgtctctt accattaaag caaatccttc
     7381 ttgggcattc gtataggtcc aagattctat ttcgatcgtc gcccgcatcc taaagttgat
     7441 gtctttgggg attgcttgat aataataggt cattccatct gatccgcttg actggaattt
     7501 tccgccgtta ttcgtggaag agagccgcac gccgtcttct aatggttcaa tggcatacct
     7561 actagcactt gtgctttctc cgaaaacaac ccctttccac tcttcatttg cttcttcaga
     7621 ttgctttttt gtcttttcat tttctgattc tttcatcata ggatcatttc cttcttccgt
     7681 atccatatct tcactagttc ttggtatttc ctccattgtt gacatccctt caatgttcac
     7741 gtgtgcattt tcttcgttca cagactgttg ttcgttagca gcaacttcaa cgacagaaat
     7801 taaaaccggg gtactgagca taaggagatt catcattagc gcactgtatt ttatacgttt
     7861 ttttaacaag tgtattcctc ccgtaacaaa ttagaaagcg tttacttaaa gtattaccta
     7921 atttattttt actgtcaata aaaaacaaaa ttataataag tgtattttat aaaaagaaaa
     7981 aatataactt aagttgttga taataaagag atttattaac gtaaaaagaa taagtttttt
     8041 taaaataacg tataaaaaag agcataaaac aaagtcgttt gttttatgct cttcttttat
     8101 tattttgtgc tttttatttt atcaaatcac tttgtttttt tataaaattt ctgcgcctag
     8161 tgtggactca aaatgtctca atgcccattc atgtccaacg ggatcgaaac ttgccacggc
     8221 atctttgaaa atatggagtt tgtatcctag attatacgca tcgactgctg tatgcaacac
     8281 acagatatct gtacatacgc cggtcaaata aatatctgtg atctggcgtt ctcgcaaacg
     8341 gatgtctaga tctgttccac taaaagcgga ataatgccgt ttgtctatcc aataaacatt
     8401 ttcctcttgc tcatagtctt ggtataaact ttttaaagag ccgtacaatt cccgtccacc
     8461 cgttcctatc acattatgcg gaggaaataa gcggttctcc ggatgaaagg aatcttttgg
     8521 atcatgcgca tcaatggcaa aaacgacaaa atccttttga tcaataaact tctttgtatg
     8581 agcagtcagc tttttttcaa ttacttgtcc tgctgtccca gtagtcagct ttccatcctc
     8641 tgcaacaaaa tcatatgtgt agtcaatcga gatcaatgcg ttcatcttag tcctccttat
     8701 acgagatgtt tgacaaattc gatcaccatt tctgctgatc gtttgcctgc gtcttcgata
     8761 aattcgtcaa agctttgtgt cgccgagtga tctgctgtgt cgctcattgc acgtacgatc
     8821 aaaaatggga tattgaactg acgagctgtt tgtccaacag cggctccttc catttcgcaa
     8881 gctaatgctt cagggaaatt cgttaagatt tctttgattt tatctggcga gtcgacaaat
     8941 gtatcacctg taacgatcaa tccttctttt gcgttcaaat tcgttgcttt tgctgcttta
     9001 accatttcac tgcgcaaata tgtgcttgct tcataatata gaggcattcc tggtaattgt
     9061 cctgggttgt agccaaatcc tgttgcatct gcatcaaaat aagctacttt gtctgatata
     9121 acgatgtcac ctacttgtaa gccttcaccg attccgccag ctgaacctgt attgatcacc
     9181 atgttcacgc cgtattgttg gatcaacaga cttgtggtaa tagatgctaa gacttttcct
     9241 ataccggaac gtacgacgat cacttcgtgg ttacctaatg aacctgaaat aaataaagcg
     9301 cctgcgcgtt cccatgataa tggttcgctt aggttctctc tcaaaatttt tacttcttct
     9361 tccattgcgc cgataatacc gattttcatc tttttaattc cttctttcta tataaatgcg
     9421 attgctaaaa aaaccacaca taacattagg ataacaataa taagagattt catcaaaaaa
     9481 gagttccatt tggtcgtctt ttcccgttcg ctgatccttg ttttcgtgat tggtttattc
     9541 tttttgcttt ctttacgata aaagctggca atcttttttt cttcttgctg ataagctgtt
     9601 tctgcttttc gctgtttttt taatgactcg cttgcttgtg cttgttgtcg cttgcgaagt
     9661 tcgcttcgcg taataagcgg tcctttggcc ataagccgat ccctctactt tcctttggtc
     9721 aatagttccc agtattgaat cgcataaatc gtctttgcat cacaaatcaa ctgatctttc
     9781 atcgcttgtt gcgcctcttc aagagttaga tgatacaact ccagtacttc gtcttcatct
     9841 tgtgctaaag gattctcgac cttctctacc ccttgtgcat gataaatatg aaggacttca
     9901 tttgcaaaac ctggtgaaag atacatcgaa gtaagatggc tcagcgactt cgcacgatag
     9961 ccagtttctt cttctaattc tctagctgct gtcatttcgg gattttgtcc ttctcctgga
    10021 tcgatttttc cagctggaat ctccaaaatc actttttcta aaggttttcg aaattgtttg
    10081 acaagaagca gacgatcttg ttcgtcaaaa gcaatgatcc cgacaccgcc aggatgaaat
    10141 accagttctc tcttcgaagt tcctccatct ggcaaacgaa cttcgtctac agcaacatca
    10201 ataatttttc cgctatagat ttctttgcga ctgattgttt tttcttcaaa ttgctcattg
    10261 ttcagcatat cttctcctgc tttctttgta atcataactg cttttttttc atttttcaat
    10321 ttttctcaaa aactccttat tagtttacca tagataaacc ttagattttc tctaaatata
    10381 gaaaatcctt ctttagtccg atactctttc tagtagtccg tatgctaaaa taagtatgta
    10441 atcgaataac gaaaagaatt caatactcaa cgataaatga catggagggt ttattatgat
    10501 aaaacgaaat tggccgtggc ttctagcatt ggtcttgcta tttctctttg tccgaaacaa
    10561 tagtttttct tttgttctag gacttttatt gataggtgta gttttagttt tgtggggact
    10621 ctttggttcg cgcaggaaaa agggaaaaca agaagaaatt cctgcactga ctgacgaact
    10681 tgaaactcac tatgaaaaaa gcggtatgac agcgaatgaa atttcatttt ttcgacaaac
    10741 aatgaatcaa acaaaaaatg agatcaatca attgcaaatg aatatgcagc agacagccaa
    10801 acttaaagca atcgatctcc gccatgatcc agtcaaagca gcgaaagctt tattcaaaga
    10861 attggtgaaa gaaccaaacc gactgcatga agcaagtcag tttctttata cgcatctgcc
    10921 gaatctagtt gatttgacaa ataagtataa tgagatcaat gaccatgaga tcaaaaacaa
    10981 acagacctat gaaaaactag aagaaagcgc gcagatcatc gaccagctgt cagctttaat
    11041 cgcacaagat tatcaaaaat tcgttgctga ggatctagat gatatcgacg tagagatatc
    11101 tgttgcgaaa cagagcttaa agcgtgataa taaatatgaa ccagaacaaa aggattaact
    11161 cttttgttca taaaagaaga ctcggaacat tcagatgatg tctggctgtt tcgagtactt
    11221 tttctaaagt agagacattc atgtaagaat cgagaacaga ttcaacaagg aggaattttt
    11281 tatggatcat aaaccgactg aaacaaaaga tgttacacca gttaacgata cacttgaaga
    11341 tttattgaac aacccgtttt caactccaac agatacgctg accacttcac aacaagcgga
    11401 gattgatcag ctccaagagc aacagacagc tgctcgttta attgacaaac taccggcgga
    11461 acgccaagaa caagctaaac agctagcagc aaaaatcgat gctaatgatg cacagtctgt
    11521 catcagctac ggatcggctg cccaagcaaa attaagtgaa ttctcacaat cgatgttgaa
    11581 tcatgtgcaa gcacaagata ttggtcctgt aggagattcc ttaactgaac tgatgtatcg
    11641 tctccaagaa gccaatcctg acgaactgcg tgccggagaa gggaacatct tccagcgtgt
    11701 cttcgggaag gtcaaacaat cgatctatga agtgacagca aaatatcaaa agattggtgc
    11761 acaaattgac aagatttcag tgaaattaga taaagaaaaa gacggtctgc taaaagataa
    11821 tctgatgtta gaacagcttt accagaaaaa caaagactac tttgatgcac tgaatattta
    11881 tatcgctgct ggagaattga aaatggaaga acttcaaaca acgatcattc cagaagcgat
    11941 gaaaagagca gaagaaactg gcgatcagat ggatgttcag attgccaatg attacacgca
    12001 attcctagat cgtctggata aacgtacgca tgatcttcgc ttagctagac agatcacgat
    12061 ccagcaagca ccacaaatcc gtttgatcca aaacacaaat caggccttgg ctgaaaagat
    12121 ccaagcatct atcgctacag ccattccgct gtggaaaaac caagtcgtga tcgcattgac
    12181 cttgcttcgt caaaaagatg cagtaactgc tcaacgccaa gtctctgaaa caacaaacga
    12241 cttattgaag aaaaattcag aaatgctgaa aatctcagcc atcgaaacag cgaaagaaaa
    12301 tgaacgtgga atcgtggata tcgagaccct tcaaaccact caaaatgatt tgatcgaaac
    12361 aatccaagag acattgcgta tccaaaaaga aggaaaagaa aaacgtcgtc atgcagaagt
    12421 agagcttgga cacatggaag aagatctgaa acaaaaactg ttggatttaa cccaatagat
    12481 cacctttcgc ttgttactaa aacaaacgcc caacaacagt ttattacaac tgttgatggg
    12541 cgtttttctt atctttcttc taacttctcc attggagtct atttctcatt catcttgctg
    12601 tgtccatagc catagccaaa ataaatgatc agtccaataa ccattacgat accgaacacg
    12661 atccatgtca cagtttgcag acgaagcatc aatacaatac aagtcaagat ggagacgatt
    12721 ggcaatagcg gaacgaatgg cactttgaat tctccagctt ttggtttgcc aaacattttt
    12781 ctcaacttca gtaagcctag actaacaagc atcaaatagg cgagcgccat gatattcgtt
    12841 agttccgcta atacattcaa tgggaaaaat ccggcaaaga atacagctgc cacaccagcg
    12901 gcataagtcg cttttttcgg tgtacgatgc ttttgatcga tttctgtcat aaacttagga
    12961 agtaagccat ccttgctgac agcaaacagt aaacgagcaa gtgaatacat catcgagatc
    13021 gttaccgtca gtagcgttaa aattgcccct acagcgatca ccgcgcccac tgttccatgt
    13081 cctacatacc tcattgcaaa agccactggg tcattgactc ctagttcagt atacggaacg
    13141 attcctgtca agatcagcgt cacgatgatg taaaggacag tagcaatcag tatcgaacca
    13201 atgattcctc ttggcacatc tttttgcggg tttttgactt cctcggctgc cattgcaacg
    13261 gcatcaaatc ccaagaaagc aaaaaacaca agagctgcac catctagcac accggaaaaa
    13321 ccaaaaggca tgaatggctg ccagttatca ggtttaacga aaaatattcc tacaatgata
    13381 aacaacgcga tgataccaaa tttgacatat accatggcat tattcaaacg taatgctttc
    13441 ttggcttcaa gcgacacgat gtaagtgacg aatatcacta cgagcaccgc aatcaagtca
    13501 atgtatgtac cattttccgg attgtacccg ccagttaatg cttgtggaag ttcggcccct
    13561 aaacttttca aaaaaccttg catatagcca gaccatccgg aagcaacaga ggaaaccgct
    13621 agtaaaaatt ccccgatcaa caaccaccct gtcagccagg cagcaaattc accaaataca
    13681 acatacagat aggcatatgg cccaccaatc actggcactc gcgaagcaaa ttctgcgaaa
    13741 cttagtccag acaaaatgac aacgattgca gctaagacaa atgaaagtga cagtgctgga
    13801 ccagcatttt gattggccgc aacccctgtc accacaaata ttcccgtacc gatgatcgca
    13861 ccaattccaa gcatgatcaa atcagacgtt tttagttctt ttttcatctc actcgtatga
    13921 ttgagattga tctctttctt acgaaacaaa tccattgaag ttcctcccca ttttaggttg
    13981 gaaaacagcg attaaactgt tttcatatac cctaaaagga taccacctaa aatgactaaa
    14041 aggcaaccaa caataacata aatgagctct tttttcgttt ttctttcgcc aaggatgaaa
    14101 atcccaccta aagtagagat aatgatcccc atttgtgaaa gtgagaagcc aacagctaaa
    14161 ccaagtgatt tgactgttag aagcatgcag atgtttccta agccccataa taatccacag
    14221 atcatgtttt tccacacaaa ctgatcgact ttaactttac ggaaagcaaa gaagcttgcg
    14281 cctaagatca ttccgatact ttgaggtaag ataattgaca tcgcatccaa attcgcccag
    14341 ttgacgatga tggtatatac accataacca atcgtagaga agatcaatgc tctaaaaccc
    14401 ttaccaaaat caagcatttt attatctgta tctatttttc cttcgtcatc ttgtcgggca
    14461 gtcaaatacg caccgcttac aagaagagct aaagcaataa taccgtatac ataatctttc
    14521 gtttgtgtcc attcatggaa gaaaacggct cccgcaatcg tattcaaaat taattgaaaa
    14581 ccagtagaca atggtagccc tacagaaaca cccatgtact tcattccatg gaactgtcca
    14641 ttttgtccaa cactccaagc taagccggat aaaaatccga tcaagaaaat ccaaggggtg
    14701 attacaggac taacaacgaa aaagacaact aaagaaaaca gaaatgcacc gatcgtcatt
    14761 cctagtgttt gttgattggc atcgccacca attttaccac ttactaaacc aatactaccc
    14821 catgccaaca tagggactaa tgctattaaa atttccataa ccaatctcct tttatttcac
    14881 atgataagaa acttatcagc gaaaacgtta acaacacaag cacttttttt cgtttctaac
    14941 gattttctaa cgtttttcat tataaaaatt taagattggg aaaaataaag tcattgttgt
    15001 cccttttcct tcagttgatt cagccgtcaa atggatacct aatttttcgg ccagattttt
    15061 tgctaaataa agtcctaatc ctgttgaatg tgttttgcta agtcgaccat ttctcccagt
    15121 aaatccctta tcaaagatcc gtttcaagtc ttccgctggg ataccgatcc ctgaatcttt
    15181 aactgaaagg ctgactcctt gcgcttcctt ttctatgctc acaatgatgt ttccgtgatc
    15241 cggcgtatat ttgatggcat tactaagcaa ttggttgaag ataaaagcga tccatttagc
    15301 atcggtcaaa acagtatgat cctcaccaac gatctcgtag cgaatatttt tttggataaa
    15361 ataatttgcc tgactgcgta tcacaggctg aatgattcct tttagtccat attcttggat
    15421 gaggtagtcc cgcgagaaac tgtcgagtct tgcataatac aaaacttgtt ctacgtattc
    15481 atcgattttt gataattcat tttctagtaa tgtatacttt tgatcgtcaa tatcgaactc
    15541 gatcgaacga actagcaatt tagaagcgga aagtggcact ttgatttcat ggacccatga
    15601 gtcaatataa tctttttggt cttgttgatt actgattgct gattgcatta cttcttggtg
    15661 ttcacggatc agctgattga tatattcttc taccagtttt tcttcttctg tcctcccacc
    15721 actcaaataa ttttgcaaag gggaagtttt ttgtatgtcg gctaattttc cccaccatct
    15781 tctcttgctc aagtaaccac ccagcagata aaaaatcaga aagacgccct caatcaaagc
    15841 taaataagca attgtgctca aatcaacttt gatgtttggc gccagccaca tcacaaagat
    15901 cgtcaatgca ataaaaaaca gccatcccaa gatcaatgtc cactgatctt tcaaatattt
    15961 aaaaaaattc atcttaaccg ctcatccttt cagggaacga tatatccctg tcctactttt
    16021 gtttcgatat atccttggat acctgcctgt tcgatttttc ggcgtaaccg attgatgttg
    16081 actgtcaatg tattgtcatc tacaaatcgt tcgtcatccc acagtgctcg cagcaatttt
    16141 tcccgactta agatctttcc attgtgcttc atcaaaataa ataatagacg atattcattt
    16201 ttgctcaaat caatgatctc tccgttgatt tccatacttc cgttgtcaac attcaacgta
    16261 atgccattgt ggctcatcat ttcactagat aattctgtgt agttgtaaga acggcgaagt
    16321 aatgcattga tttttgcaat cagtacatcg atttggaaat gttttgtcac aaaatcatct
    16381 gcccccatgt tcatagccat gatcatatcc atattcgtat tcctgctgga gataaaaatg
    16441 atcggtactt tcgatacttc acgtattttt tgacaccaat aatagccatc caaaacggga
    16501 agattaatat ctagtaatac aagctgtggt tcttctcttt caaagtctga gaacacttga
    16561 ttgaaatcgg tcgttccaaa ggtttcaaat tgccatttct caagttcctc catgatcaat
    16621 tgtcgaatga tctcttcgtc ttcgactacc atgatttttg ccataaaaaa tccctccatc
    16681 tttcttcttg ctactacaat atcaaagaag cccttgtgaa acaagttggc ttacctggaa
    16741 aatgtcattt tagcaaacgt taaaaaaaac tggaaatatg cgatttaatc gcgtttgtct
    16801 cttccaagca gcttaaattt attatgttat tcttaataat tggtctatta tctgttattt
    16861 ttctttcgtt gtttttgtct tttaaatttt cgataacacc aataaccgaa aagcgttagc
    16921 actagcaata tacctagaag gagtataagt aatcgataaa gatggtattt ttttgtctgt
    16981 tctatctttg aagagacttc ttctgtaaag ggaactcgtc ttcctgtcac tagtaatcgg
    17041 tgagtgttca cggtgtaagg tgtacaagtg ataagtgtga ctagatcctg cttttctcta
    17101 attgctaatg tatttgtttt tgttggaagg acaacaattt tctcaacgac ttcataagcg
    17161 agcatccgac catacacttc aagatagaat cggtcatctt gctccatttc ttcaagatcc
    17221 gtaaagagtt ttttctcagc aataccgcta tgggcggtaa tcacggaatg tgtactctct
    17281 cctccagttg gaaaagaggt gccttgcagg actgtcgctc ctctatctaa caaaagatca
    17341 ttcgtttcat caaataaagg caagctaaca ttgattttcg gaatatagat ggctccgatc
    17401 atatgttgtt cgtaatattc ttttcctgga ttttggacat ttctaaccgc ttgctcaaaa
    17461 ggatctttga cttgtcccat tcctgggatt actgatgtat gagcaagcgt ctgatttttc
    17521 ttttcatgca ctgctaattg ttgctcatgt ttttcttctt tatcgcgttc aagctgcttt
    17581 tgataattct cgatccttct ttgatccaaa taattgctga gagcatctgc taaaaatgga
    17641 tatgtaaaaa ctgcagctcc ggcaagaaac atcactgcca taaataattg taataaaaga
    17701 ctgatttggg tttgaggtct attacctatt ttctttttca ttttcttcac cttcttattt
    17761 aaaaaaactg cagcaacctt tataaaaccg attgctgcag cttatttttg ttgcttatac
    17821 ttcagacttc attttagatt tacggtacca gctgtacgct ccaatcatca aacttattcc
    17881 gattagtaag aatgccaaga gcccgttccc accagtagca ggaagtgacc cgcgtttcat
    17941 attcggtact ctttctggcc ctggattttc tactcctacg atctgaattt cttcattata
    18001 gcttccataa gagatgataa attcagtaaa ggctgcctca cctgttggaa gtacataacc
    18061 ttctggcgcc atggtctctt tcatttgata ggttccataa tctaaccctt gaacctcaaa
    18121 gtatccattt ccatctgcga caaattctgt tgcttgtccg cttgttgccc actcaattct
    18181 atctgctcct gattcataaa cagctaaagg cgactcccct ttataaaata ttgcgtattc
    18241 agaacggtct tcattccaca attcgaatct tgcatcttta agcaattcac tgcttgatgc
    18301 atcatatttt tcaaactttc ttccacctgt tgtgactttc tctggcggct ctactccttc
    18361 ttcataatca aattcatcat ttcgtccgat ttcaataacc gctgtgttgt tatggaaatc
    18421 atccgcttct agttctgtgg aaaccttcaa attatacgtg atcgttaaat ctcctccacg
    18481 tgcatctgct aatctaccta aagtatctac tttggctact ccattttctt ctgtcaaaat
    18541 cgtcacgaca aacccgttgc ttactacttc tactatataa tcttgtcctt ttgttaaaat
    18601 cgtctcgccg gctcgcactt ctatttcctg atcataataa gttagaccaa ctgtaggcgt
    18661 atctgtgatt ttgtaatttt tgataacagc gcttccgttt tcaaggacag aatcgatgaa
    18721 ataaggaata ggtgctgtga tcgtgtaaga agccgttttt cctctttcta gatcaatata
    18781 agaaatcgtt tcttgttctc cagcttcatt aataatcgtt acttgtctaa ggtcgctttc
    18841 ctctacattc atctgtttat ctgcttctct gatttcgttt ttcggataca aatgcacatc
    18901 tgtattgtat gtgtctttcc atgtagaacc atcccacacg ccatccacca tatcaggcat
    18961 catcactggc atcataacaa ccattggaga cgcgattctg gaaatatgag ctggcgaata
    19021 agtttctaag aaaagataga ctttatctct agaagaaggt gcttcttccg tcacttggac
    19081 ggtagagaat accgcttctc cagcttcatt cgttgtttgt ttgtcaatac ttgtcagtcg
    19141 atattttaaa aaccaacttt ctgtgtgccg tttttgaatc cattcaatcg cttcggcaga
    19201 attaagaccg tcttctcgtt ccggtgttaa aggattatct cgaatcaaat cgtaatatac
    19261 ttccgttacg tcaaacaaat caaaatcgac tcctggaata ttttcttcac caaaatccgt
    19321 agaaaccaat ccgctattcg gtcgttcttc cggaacagat gagaaacctt ttttgtgtaa
    19381 tgtaatcgtc acttcacttg gtttttgtgc gacttcttcg gctttgacta gttttccgtt
    19441 cccgaaaatc cctattcccg cagtaacgag taacagtaca ctcattcccg tgacaatcat
    19501 ttttttattc atttttttct ccccgttctt tttttattaa taaaacagca cctaacaata
    19561 aaagaattcc tagtaaagaa atcaacgttc ttgcttcatt cgtttgcggg aatacggacc
    19621 attttcctga ttgacctgga ttgcttggac cactcggctc tttcggtttg tccgttgttt
    19681 cttttacggt ctcgttataa acacgtggtg ttgcggattc ataggcagag actggaactt
    19741 tcccggattc taattcatcc atctcctgtc cgttaactac tacatattgt ggttggcctt
    19801 ttgcatccac ccaaaattca gggatgatga cctcgattct cttacttgct tcatccatct
    19861 cgtaaccttc cacacctttt aattcttcaa aataataggt gccagaaggc aagaaacgtt
    19921 gtcctgtcgt aacaaggccc tctttatccg aaatgaattt gcttacattt ttgtcattca
    19981 aaggatcatt gtctgctggt ttgatccatt gatttttcaa gtcattagca ggagccatat
    20041 ctaaataata tttcccccct tctatcatct gatatagagc aaaaacagca ccttctaatg
    20101 gttttccttt ttcagttgtt cctctttgac gtccatactt gaagaagtat ggatctctta
    20161 gataaccgac atttttagga tagatatgaa tctcttgcag ttcctcgctt gattctgtcg
    20221 ggtgataaat tggcaaaata gcagcaattg ggatagctgt ctgatcgata tcaacatcca
    20281 atccaacttc tggatcaatc gattcttcga aaatcaaata aactgcatcc cgatcttggt
    20341 ttttcttagg tgctttgata cgtgcaatcc cgtctcctaa tccgccaacc gtatctttcc
    20401 cagttgttac cgaagtccct atttggacaa gcctttcttc tgtgattagc tgacggatat
    20461 tgtttctgtt cgtttcagcg atttgctgga cgaattcttt aggagtcatg ttttcagcag
    20521 cttttttttc atgataataa tcggtcatgt cgtaaatggc aaatgtcgct ccatttagtg
    20581 gcaccgtctg tgaaatgata tctgctgttt cccctgcttc ctcagagata gccaagccat
    20641 cattttcatg gtattcaaat tgatcgttat aatcgatatc tcgaatcatc cgtttatgca
    20701 acacgaattc cacgacatct tcccctgatt cttctgccga catcgcctgt ggaagaagga
    20761 ctgctgcaag cggtagtaaa acaagtaatt gaagcatcaa acacaacagc tttttcatta
    20821 ccttgctccc tcctttttag attgtctgac aaacaaacca taggctgctc ctgcaagcgc
    20881 catgaagaat gcagcaagca ggatatagaa taatcgtccg gatcctcctg tagcaggcaa
    20941 cggattttca ggatcgttcc cgacactaag ctgtaatctg attcgatttg tctccaaaag
    21001 ttcatattct aactcttgct gtagatcagg cgtctctgct tcttcgtttg gataatacga
    21061 aatctcaact tttagtgtac ccgcttcttt tcctggaact acccgatcat tttctatgtc
    21121 aggttcttct cgataactaa tttctaaaat aaagtaacct ggaagttttc tatagctgtc
    21181 aggtgacttc acttccttga tccgatagac cccttccaac aagtctgaag cattgaatag
    21241 ggaagaatcg tttggatctg gtgccaagcc tccctcgatg atttcatact ctcctgcact
    21301 attttgtttc tcaattgaaa attctgcgcc ttcaagacgt cggctattca tactgtcaat
    21361 tttcaaaagt tccaaatcga ttggcgctag ttcatcttct aaggttactt gtagtacacc
    21421 atcgatgatt tcatagcctt ccggcaaggg agcagtttcg tcttcgatga ccgtatcttc
    21481 aatgataaac ttaccttctt cattcagtgc aaactggaac acttgatcag atagccgata
    21541 cccatttggt gcgacagatt ccgagacctc atattcacca atagacagat gcagaggttg
    21601 gtcgtaattt aaatcgacat acttttctac tcctacggca cgagaataga agtatccgat
    21661 cccaggcaaa cgtttcgatt cttctggttc taacggatta ccagtgtatg ctcgatacag
    21721 cgggaaggaa gaatccccca ctttcctgat atcgaaacct gcaccatcta atgtatggcc
    21781 ttcatggtct aattttctaa ctcggatctc ccgataatca aacgtattgt aagcagtgaa
    21841 tgctacccat aaacgatcct cttcatctgt tgtatacgtg agatattgcc aattttcttc
    21901 acctgtttgc ttgagttgga tagtccctat agcattatcg taaagtcgca agatccatgt
    21961 gtcattttca tcaggttctt tgaaaccttc tgggttctgg ctctcaatga ttttgtattc
    22021 tgcatagttg gtagtttctt cactatcctt ataaacaaat gaatctggct ctccgattgg
    22081 atgaataact ttctcaccat ccacgattac atatttcccg tcttcatcta ctgaatagaa
    22141 cgtgagtctc cctttctctt cctctacttc gttcacacca gaaagccctt tcgctactgg
    22201 atatccgatt tccagtccgt taccatctgt ttcataaagt tcaaactctg cgccattgat
    22261 tggcatatta ctgccttcaa ttagtttatt gatattcatg tggattggct tgaattcatt
    22321 tcgcatttcc agtttgacat acaactcatc atactctgtg ccttctatgt taggctcttg
    22381 tacaacagaa agcttgtgat attctccttc ataataggaa ttcggatctt taccttccgg
    22441 tccgccgact ctcgaaccag atgtatattc atctacaagc tcgtagtgac ctaacatctc
    22501 atcttcgttg gctgcatatt ttttgatttg gaattgatac gtctcatata ctaactgata
    22561 acgtggtggt ccagcgatct ctctgatcca aaatatgtcg ctgactgctc cagtttcatc
    22621 tcctaaaatc ggcatcttcg tttcaaagat cgctattccg tccgtggtag ttttacttgt
    22681 ttcgattggt tcggcttccc cactctctgg attttggtca tataatgcaa attcgactcc
    22741 agccaacggc tcgccatttt gacccttctt gctgacttgg aaattacggc ctttatgttc
    22801 gtaacgaatg gaagaagatg ggaaatacat ccgtccagta gtgatatcat tctcataatc
    22861 ctccaggttc tgaatatagg ccatattgtt cactgtgtaa aaatttccgt cttgagctcc
    22921 cgcatcaagt tttacttggt acttgatcct gaccatttcc ccttcaccta aagaaatcgg
    22981 agaaaattta taagcttggt tttcccgtcc gacatacgtt gtcggagtcg ccactacttc
    23041 tggtggggct tgtccatgac gttcttcatc ccatatttct ggctcttggt acttcatgcc
    23101 gttttctcct ggatctagat ggtaaaactg aatatctgcc gatctgccag ctccaaatgg
    23161 tgagacatta aaaccagttt catcataaag tgtggcttgc tggatcgttc tcttgaaggt
    23221 agaagagata tcctccaagg ctgtaacaat gtcattcttg ttgttcgcat tgtaatatcg
    23281 cggtgttacg ccatcttctt ctaaatcagc aatatttttc aatacatttc gtccaatagc
    23341 atctagtcgt tgtctcccgc taacgtttcc atcgattcct aaaccaatgg agaagatact
    23401 tggcgggttc tcttgatact tgtttttcag ccaatgatga taagcaactg tgttgatcgc
    23461 agatgacttt tcttgcgaag taggactgcc attcgtattg ttattagaag aaccatcacc
    23521 aacccagtga gtcgctttat tatctccttc ttttacctct ccataacgat agtgtacatt
    23581 tacttcatct tctggacggt taaagtcaga tgcataggta actggatgag taaaccctgc
    23641 attagaagta ttcccagaaa actcctcaaa atttctgaac aaaacgtcat tatcttcagc
    23701 aatcttatct tccatcacag tccagttgct ccaagctcca ttagtgctcg tacgataact
    23761 tgactgaatc ggtgcataac tgaaggtcgg tgtcccatct cccacaacga ttaggatttt
    23821 ttctggattt ctattttctc cgccattgtc tttatacaat gtttcatagc catttttaag
    23881 ccctagtgta agcggagttc ctccgattgg tgtacgattt aatgctgtat tctcacttcc
    23941 cgtcaaacga ttgatatcat ttgtcaatgg aaagatgtcc gaactgattt gctcatggtt
    24001 attgatagct gtactattat ggtaaaaatt caccataccg attcgaatat ccagactcgg
    24061 gtcagagagc agatctttac tcatttcgtt tactgcctca atcaacgcag catttttagt
    24121 ttgactattg gtcccagcgg ttaattcact catactcgct gacttatcta gaacaaaaac
    24181 gatatcgatt ggactgattt cctttttctc gcccccgatg acatctagat agacattgta
    24241 aagggttgtt gcatcttcag gatgtagatc agatgctgtg ctgcttggca gaacaggttc
    24301 tacccatttt ttgatgtacg ccccgttata atccaagtaa ctgttttcaa aattccgttc
    24361 atttccccaa agctgaacgg tttgagctgg aacgtttcta tcttcttcgc ctgcagttga
    24421 tccattatta gtagcatagt cgtaatttcg ccagttcgct cccccgtttg tataatcact
    24481 atcatcgatc aagttctctc cgtcatatgg cgagcctttg tctccttgat agcgttcatt
    24541 gctttgtgtc caatttccta acacttttgt atagtcattc gttgtaaatt tcgggtagcg
    24601 atgctgttcg ttttcaactt ctccgctagg atagatatat tcataggggt cttgcgtagc
    24661 tgttggccca agacgctcca aaataccgtt gaataactgg cgttgaatgc gctggagtgc
    24721 ttgaccgctg ggtacgattg gttcgtccag cttttccatc tcattttctt cgctacgttg
    24781 aatttcagcg gaatcttctg tatcagtttc attttcttgt aattcttggt tatctattct
    24841 ttcatcaacc accacttgaa gaggtagttc cccttcgttc acatcgggga tcttttctgt
    24901 ttcaaaaatc aatgtgccgg tttctgcttc ttttgaataa acttttccga cataccattc
    24961 ttgaccatcg atcgtctcga cttttaggtc ttctccatct tcatcatagg aggatagacc
    25021 tgttccacga acgtttttta cggtaccaat cccggcggct gccgcatcga ttgctaaacg
    25081 tattctacct tcattggcac tttccttttt ttcaaaagcc agatgccatt caagggtttc
    25141 ttctttttca atatacttcc cttcaatttt tgcatagtcc tcatctaaga atacctcatc
    25201 ctcctttgtt tctgttgact cagcagctac atccagggtc gtcatatgat agactgactg
    25261 gaaatatggt ccaaatagca aactgacagt tactaactgc aaaagcttca attttaaccc
    25321 tttcacatcc ttatactcct ttcaaaaata tttgtcttct ttcacaacaa tcaacaaaac
    25381 aatgcccatc taattataat ttaatttaat tttaatttta aatcaaatga tatccttttt
    25441 agttaaaaaa caccaattat acaacattta ttattaaaat taaattataa aattttataa
    25501 cgttctcatt taacaaaact taagttatta agacaaatga ataaacaact acgctttcct
    25561 cgaaacaaaa aaataaagca caaatgcgca tttacgcact catgctttat tcgtacaatt
    25621 aattcagata acggcagcgc ataggatgcc aataacactt cctaaaatag ctcctcctaa
    25681 aatgtcagta ggataatgta aaagtaaaaa aatcctcgaa taagcgatca aacaagcaaa
    25741 taaaaaggct aatattcctg caactggact taattcgaac aacgcagcta ctgataaaaa
    25801 ggctagcgct gtatgtcctg aaggaaaaga agaagtcttc ggtttacttg tgtaaaggac
    25861 aagccgacga tttttttcat aaggtctttt ccgtcttgtc gtttttttga taaaaagcaa
    25921 accaacaaaa aaatagatca aactgccgat aagaatggct agaccatatt ctctagtatc
    25981 aggtatcaag actaaaacag cagcaaggag taaccaaata aaaccaccat tagagatatt
    26041 actgataaat ttcaaaaaat tgatttttcg actatccgtt ttccgttgct tggcgaactt
    26101 gttgataata ctatcatcga ttttagcaat caaacgcaac atgttattcc tcctttttaa
    26161 gaaccttttc ttgcgtacga tggataaaaa gcaggacttg aaaataaaac aagaaactga
    26221 aacctaataa agcaaaatag attggtcctg caaaccagaa tgcgacaaga aggatcaacg
    26281 tgcctgccaa atagaaaagc gcgcttttga aatcattaaa aaacaagaaa aatgcagtaa
    26341 tccaagaagc ttctttttgt tccagttgaa gcaagatttt gtaactaaag agaagcgcgc
    26401 ttaatagaag caaaaacaac gtagtataca tcaatgtata gccaattccc ccaccccaat
    26461 agcggaaaat ctgataatcg atcaatagaa gaagaaaata aacacttgct aaactatctt
    26521 gctttccata aagagagaag ttctcacgaa actttttcca gaatagtgaa ccgagctttt
    26581 tctcttcatt ttttttgtaa tgataaatca tatacagaac tgttcgactt gcagttccta
    26641 atgtgaaaac aggcaagacg cacaggaacc atagtagctg taaatggata agacgtggaa
    26701 tccattcaat gatttctaag aaataatcat tggctttttt accaatcgat cgattcatca
    26761 tccttttgaa cctcctgtca aatccatccc ttcaatgaaa taacgttgtg caaaaaagaa
    26821 cagcaaaatc gtcgggatca agacaagtgt tgctcctgcc atcagataat tccattgtgt
    26881 cgttgcttgg ttctgaaaaa cttgcaagcc aatctgtaac gtgtattttt cttgatcttg
    26941 gatatataac agaggaccta agaaatcatt ccaagctgcg ttgaaggaat tgatcgcaat
    27001 ggtaatcaag gcaggtttcg tcaatggaag catcaaatga ctccaaatat acaaatgatt
    27061 cgctccgtcg atttcagcag cttcgatcaa ttcattattg atcgataagt aaaattgacg
    27121 catcagaaat atattgaacg cattgccgaa gaacgatgga acgatcaggg gcaaataagt
    27181 acctacccag ccgatcttgg agaataatac atattgaggg atcatcgtta caaaccccgg
    27241 gatcatcatc gtagaaagaa ccaaagcaaa catcagcttt ttcccaggaa aatcaagctt
    27301 cgcaaatcca taagcaataa aagaatttga aaggacatta ccgatcacta ctagcacagt
    27361 gatgaataat gtatttcttg catagcgcca aaaaggaaag gcggcaatcg tatccagata
    27421 gttttcccat acgatttttt ctgggaaaaa ggtcggagga taacgaacga tttcttccat
    27481 tggtttcaat gaagtaaaca ccatccaaag caatggaatg atcaaaatga ttcctattac
    27541 tgtcatgagg acataactta gtattttttt tgtttgccga ttcacactgg tctttttgaa
    27601 ataatccatc atttgccgtc acctccagaa taatagaccc acttaggtgc aagcttcaaa
    27661 ttgacaaacg tcagaaccaa cacgatcaga aacaggatca ttgacatagc catcgcataa
    27721 cccatatcaa aagctacgaa cgccttgttc cacatatgca gattataaaa aagcaaagaa
    27781 tttgcaggtc cgcctgttcc atttttcgtc atgacataag cttcttgaaa aatttggaaa
    27841 gaaccgatcg tagaggtgat cacatcgtaa aaaataatag gagtgattaa tggcaaagta
    27901 atacttttga actttcccca aacgcctgct ccatcgattt ctgctgattc atataaagat
    27961 ctaggaacat tttgtaatgt agctaagtaa agtagcattg ctcctccaga actccatatt
    28021 ttcatcaaaa tcaacgcagg tttcgtccat tgtggatcaa acagccaagc tggtccaggg
    28081 atatggaacc agcccagaaa tgtgttgatc aatcctgcag atggagaaag cagctgcatc
    28141 cataagatgt agactgctac tcctgataag actgccggta aatagaaagc tgtacggaat
    28201 acactgattc ctttgatttt ttgattgagt actgtggcta aaaacacact gaaaatcgtc
    28261 gtcagcggta cattgaagat gacgaaataa acggtgttgt ataacgattt ccaaaataaa
    28321 tcatcgtgaa cgaacattct tttgaaattt gctagaccaa tgaaattcat cttggatgtc
    28381 aaattataat cggtgaaact accaataact gaaaaaagca taggccctag cataaatacc
    28441 aagaacccga taataaaagg cgagataaat aaataggccc atcttgcttc ttttttcttt
    28501 cggatagata aacgcttcaa attttcttgc ctcctctgta cctagacagt caaaaatgga
    28561 ttattgattt tgcttgacta agttttctac tgctttttgt gcatctgcca atccttcttt
    28621 tggtgatttt gttcctaaaa gaatctggtc aatctgttca ttcactaatg tcgtgtaatc
    28681 cggagcatct aaaggaactg gtgtgatcac cgtattttta aagttttcgt ccgccatttg
    28741 atagatcatt tttcctttat ccgttaggtt gtcattattc actaaattct cattggctgt
    28801 cttgctcgcc atgatatcaa aacttttttc gccgaatttt tcttgtactt ctggagaaga
    28861 aagatatttg atgaattcat aagaggcctc aggatctttt gctccgtatg gtacttccaa
    28921 gacgaatccg ccaccccacg accaatggcc tgaaccttct tctttttctg ggagctgcat
    28981 cacgccaaaa tcaaaatcat ccggtgcatt ttccatcaaa ctagagtaat aattgatatt
    29041 ttgggcacgc atggctacta atccggaaat aaatggatca gatacaccag acccaaattc
    29101 ggcttctaat ttattgatcg tatttttccc ataataatct tgccagtctt ggatccactc
    29161 caaggcttcc actttatttt tagtatcaat cttgactttt tcatctttgt caaaccaact
    29221 agtcccgtta tctgcgttta gcgcccatac atcagcccct aaattccata atggataaaa
    29281 accgatgcgt gtaaattctt ttccttcttt tttatctagt ttacgtgctg tttcttctaa
    29341 ttcatcccaa gttgtcggta gatcttgttc actgattcca gcttctttaa ataacttttt
    29401 attataaaaa atgacttgtg tatatgtatt gaatgggatc gcataaggat caccttcata
    29461 ttctactgta tcccaaagct gcggataaaa atccttagaa gtgtcttttt ctacatattt
    29521 ggagagatta gttgcttgct gtgcttctgc tctttgggca acggaattta tgtcctgtac
    29581 aataacatcg ggtgcatttc cagctgttat tgcagataaa gatttagtcc agatgtcacc
    29641 ccatggctga tacttataat ttacatggat ctcgttttgt gattgattga aatcctcaat
    29701 gatttcttcg atagcttctt ttcgtgcacc ggatccccaa aaggaccaga aatcaatatt
    29761 ttttcttcca tcacttgttt tttcagaaga atttgctgca cttgaactgg catcgcttgc
    29821 attacttgat ccgcatcctc ctaataaaaa caatgctgaa cttgccagca accctgtaaa
    29881 caataatttt ttcatatttt acctccaaat tttaatgtaa ccgatgtcgg ttttaatata
    29941 acgatatcat tttgttctga aaataacaat ttataaggta tgattctaaa aaaattatta
    30001 atgataccaa tactttatat aattcgcaag cgttttcttt gaatttttta atctttcgta
    30061 gcagaatgag aatgtattaa aaatagatta ggaggaattt tgatgtttag acaccagaaa
    30121 gaattgcagt ttgaagcaaa accagatcgt ccgaatccat taatcgcgaa atatctgcag
    30181 gagctgatcg gcggccaata tggcgagatg agtgtagcta tgcagtacct gttccaaggc
    30241 tggagctgcc gaggagaaga aaaatataaa gatatgctga tggacattgc aacagaagaa
    30301 atcggccacg tggaaatgtt ggcgacaatg gttgctcgtt tattagaggg tgcgcctgtt
    30361 gaagatcaag aagcagcgat gcaaaaagat ccaacaatcg gtgccattat ctctggaatg
    30421 aatccacagc acgcgatcgt agctggctta ggacctcgtc ctgctgacag cgtgggcaat
    30481 ccatggagcg gtgcctatat cattgcgagt ggcaacttgt tagcagactt tagagctaat
    30541 ttaaatgccg aatcacaagg aagacttcaa gtagcacgta tttacgaaat gatcgatgat
    30601 cgtggcgtaa aagatatgct gtctgttctc cttgcaagag atagctatca tcaaaatcta
    30661 tgggaatgtg cagtgaaaga attagaagaa aaagaaggtt caatcttagt cccaagtact
    30721 ttcccgcgtg acaaagaacg aaaagacatt gcttatgact tctataactt ctcagaggga
    30781 gaccaaagta gtgaaggttc ttgggctagt ggcaaagcac tggatggcga aggcgaatat
    30841 caatggttga aagaacctaa gatcgaaggc gctgcaccaa aattagaacc agtaacacca
    30901 aaaatgtatg ggacacctcc attcaagaaa taaatatcta cccgtagaaa cacccgtttt
    30961 ttgttccaca cgctttgtga gcaaaactga ctaaagtcat taatcataac cacccttcaa
    31021 aagggtggtt ttctctagag ctataagctc aaatcaccgg ccagcgccta aagacgctgg
    31081 ctttcatatt ttatgttcaa gccatattgc ctttgccagt tttgcccacc tcttaaaagg
    31141 tttatgtact actccttagc ccttaaaagg atcgtcttac tctcgtacac tcagcttatc
    31201 taacgcacta tcatgttttt cttgttcttg aatgtacttt ttgatggtcg cttcattttg
    31261 tcctactttt gtcacatagt atccttccac ccaaaagtgt cgatacccaa atttatattt
    31321 tagatttgca caatctcttg atgatctcct gaatattggc tcgatattga ttataaatta
    31381 tttttcttct atactttggt gtgaatacaa tgtggtattt acacatctac tttgtacggg
    31441 ctaaactatt tccctttatt gctccacccg catagcaagt ggttttgtat ttcaatcgct
    31501 cggcgcgctc aattaggtct aaagacaata aatctaaaaa cccgctgctt cactgacaga
    31561 aacaacgggt tttcagaaaa aaatgaaatg aaaaagatct cttacggcaa tctattttat
    31621 ttttttgatt ttttataaat ggaaaatgct tcaagaagtc caagaaataa agccgtataa
    31681 aaaccagaac ctatgaaatc tttatttatt agatattcaa cagaaatccc gataacacta
    31741 gccaacaata tatttatcag aagtgtaaaa taaggatgct ttttatagaa tagaactagt
    31801 gttgttttca tagactctcc tttcttcata tttccaatta atcacaaatt cacggcatta
    31861 ctagaaaaat taaaggggcg cagaagtgga aaaatactct tcgactttcc ctacaaacca
    31921 ggccatcttg tgtttaataa ctataccaaa tctaaggtac ctctaaatct aaattccatt
    31981 tgtggcggtg acctccaatt tttaactctt ctatctccca tccagtatac ataaccttga
    32041 tacttcgacc acttatatat cgaacacaca aatacctatc cattgcagga gtactaccta
    32101 acttagttgc tacttgcatc taaataacag ctgaaaagat aggacttact cttagtaaag
    32161 tatctgaact attaattttt aaactatcat ttttatcctc aactacatgc acatctgttg
    32221 caacttcact ttctgtctca tccagataaa tgattaagga atcgggaatt gcaatatctt
    32281 cagctgacgc tggaatagtt gagctaaatt agtcatcata tctttattat aaaataacca
    32341 acagcaaaaa atacaatgaa aaacaaactt ggaactaatg tatcattaat aaatgttgaa
    32401 aaattcagca actcattact gtatgatttt agcagcccaa ccaacatata ccctataaaa
    32461 gctaataaga tggttaggaa tcctttagaa tttttactca ttcatagaca cttccttaaa
    32521 taacttttat tatagaaggt attgtaaata ataatattga caaaaagtga ctcaaaataa
    32581 aaaaaacctt acattatact ggaatgaact gtattgatag tgtacctaaa agctctactg
    32641 acataagatc gtgtaatcgg taaagtaatt ttattaatta tacgggatgt aatcaattag
    32701 agaataaact aatcataaaa ggtactttta cattcataat tgtcgctttg agattctact
    32761 ttggttcact atctgaccca attgcacatt ttcgatcatt acaagtattg gtgtcgttag
    32821 aacaactaaa taaaaactag cggtagattt ttcagaatct atgctaattt ctgtatggat
    32881 gttccagtga tggcaaattt ctcttttcat tgtttttctt gtcgcatgat atgccttttt
    32941 gtgttattca gttaaactat agaaattctt tcctagacag atcttgatcc caaattttac
    33001 gtttttgagc atcgatatag tttaaaatga cattactcat atctaaagag tttagatttt
    33061 ttaggactaa aactgtcagt tgttcttttt cttctttctg ttaaagtatt gtcgcgactt
    33121 ttcgtatacg ttaagatatg tcgtgaaaaa acaagttttg tgacacaaaa aaacgaatga
    33181 cttgaggaaa tcacctc
//
LOCUS       DACDEG010000019        31645 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.019, whole genome shotgun sequence.
ACCESSION   DACDEG010000019 DACDEG010000000
VERSION     DACDEG010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.019"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..952)
                     /locus_tag="IV142_001010"
     CDS             complement(<1..952)
                     /locus_tag="IV142_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8484683.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELRNNKNYCPPIIDKKLFYAVQKALPKNLKTNAKRDYIFKGLLKCSDCQGSVA
                     GQTIKARYKKKDGTESIYERTCYRCVKRRNNKLRCTNKRAF"
     gene            complement(1018..2307)
                     /locus_tag="IV142_001011"
     CDS             complement(1018..2307)
                     /locus_tag="IV142_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAP8484684.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTSLVTIYKSVI
                     IPNIDETSSSEIKNKLSLLKIKEKDFILDGAINIPSYLNNREANNIKKKMLIPLESEI
                     TGLLNKLTISNVDSTREKIIRTFDKINKLFKNDEAEFKKELLELKLQELELNYSYIVK
                     VNDEKEQQKAIKEQMIEEEKVRREIEREKKRIDKEERQFNSEITKLMAYMQKASADVE
                     KELYADKIKELEEKLRELETVKEDVLQRELNTRAGYVYVISNIGSFGEDVYKIGMTRR
                     LEPMDRIKELSSASVPFEFDVHAMIFSEDAPTLENQLHSHFRKNEVNKVNQRKEFFKV
                     SLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLELTDV"
     gene            complement(2394..3098)
                     /locus_tag="IV142_001012"
     CDS             complement(2394..3098)
                     /locus_tag="IV142_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HAP8484685.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
     gene            3298..3564
                     /locus_tag="IV142_001013"
     CDS             3298..3564
                     /locus_tag="IV142_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8484686.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            complement(3732..3962)
                     /locus_tag="IV142_001014"
     CDS             complement(3732..3962)
                     /locus_tag="IV142_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP8484687.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            4034..4189
                     /locus_tag="IV142_001015"
     CDS             4034..4189
                     /locus_tag="IV142_001015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484688.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            complement(4498..4722)
                     /locus_tag="IV142_001016"
     CDS             complement(4498..4722)
                     /locus_tag="IV142_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP8484689.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            4989..5324
                     /locus_tag="IV142_001017"
     CDS             4989..5324
                     /locus_tag="IV142_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484690.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            5557..6498
                     /locus_tag="IV142_001018"
     CDS             5557..6498
                     /locus_tag="IV142_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAP8484691.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESSIRQVDNEIISELGKREASIGITQRNIISWKLVR
                     TRRMNTKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            6500..7390
                     /locus_tag="IV142_001019"
     CDS             6500..7390
                     /locus_tag="IV142_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAP8484692.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            7433..8233
                     /locus_tag="IV142_001020"
     CDS             7433..8233
                     /locus_tag="IV142_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP8484693.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            8248..9099
                     /locus_tag="IV142_001021"
     CDS             8248..9099
                     /locus_tag="IV142_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8484694.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            9096..9413
                     /locus_tag="IV142_001022"
     CDS             9096..9413
                     /locus_tag="IV142_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484695.1"
                     /translation="MNLTKQEKRICRQIMLQIIGDRDMDELSQQESRMVRKISKLIGA
                     DTMDRSKPLPKLNFESFTFEKYSHLREVGYSVNAIRKALGVGSSVWAKWRKENVNEVN
                     QND"
     gene            9406..9588
                     /locus_tag="IV142_001023"
     CDS             9406..9588
                     /locus_tag="IV142_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484696.1"
                     /translation="MIKLIGKETLKVYYVAETKAEVSRWLLKMFNKTRANASVSQKAS
                     VSETGMPEAMWISRRN"
     gene            9620..9709
                     /locus_tag="IV142_001024"
                     /pseudo
     CDS             9620..9709
                     /locus_tag="IV142_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            9784..10251
                     /locus_tag="IV142_001025"
     CDS             9784..10251
                     /locus_tag="IV142_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HAP8484697.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            10779..10851
                     /locus_tag="IV142_001026"
     tRNA            10779..10851
                     /locus_tag="IV142_001026"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10812..10814,aa:Lys,seq:ttt)
     gene            11237..11497
                     /locus_tag="IV142_001027"
     CDS             11237..11497
                     /locus_tag="IV142_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484698.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSVFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            12102..12935
                     /locus_tag="IV142_001028"
     CDS             12102..12935
                     /locus_tag="IV142_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HAP8484699.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFETLSDEEKDIYSSLNDNPSFVLSEEIRLLKIRQFRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKNPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            12928..14343
                     /locus_tag="IV142_001029"
     CDS             12928..14343
                     /locus_tag="IV142_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase B"
                     /protein_id="HAP8484700.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            14355..15884
                     /locus_tag="IV142_001030"
     CDS             14355..15884
                     /locus_tag="IV142_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8484701.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            15976..16854
                     /locus_tag="IV142_001031"
     CDS             15976..16854
                     /locus_tag="IV142_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAP8484702.1"
                     /translation="MMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            16856..17173
                     /locus_tag="IV142_001032"
     CDS             16856..17173
                     /locus_tag="IV142_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484703.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            17222..17923
                     /locus_tag="IV142_001033"
     CDS             17222..17923
                     /locus_tag="IV142_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAP8484704.1"
                     /translation="MVEWWGATIKTQSNAGRENESWGERRMSMKHKSLMPMNLQYFAE
                     GDDQKFSFDDFKSFVESNEEAQKFIQSQSQSVADKQLEAWKQNNLDKIKQDTIKEYEE
                     SKKNKSPEQIQLEKLQAEFEAEKALRVTSDNKAFVAEQIAGLELDGELKESISQFMLN
                     NLVSSDTDFTKNAVEGFTSVLNAINEKHADALKELQMKSAFGGTQQSNNQVQQNNETL
                     TNPEEQLGQILQQFN"
     gene            17938..18828
                     /locus_tag="IV142_001034"
     CDS             17938..18828
                     /locus_tag="IV142_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HAP8484705.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLFGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            18851..19066
                     /locus_tag="IV142_001035"
     CDS             18851..19066
                     /locus_tag="IV142_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484706.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            19078..19419
                     /locus_tag="IV142_001036"
     CDS             19078..19419
                     /locus_tag="IV142_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484707.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            19419..19790
                     /locus_tag="IV142_001037"
     CDS             19419..19790
                     /locus_tag="IV142_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484708.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            19783..20178
                     /locus_tag="IV142_001038"
     CDS             19783..20178
                     /locus_tag="IV142_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAP8484709.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            20180..20557
                     /locus_tag="IV142_001039"
     CDS             20180..20557
                     /locus_tag="IV142_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484710.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            20558..21166
                     /locus_tag="IV142_001040"
     CDS             20558..21166
                     /locus_tag="IV142_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8484711.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            21166..21507
                     /locus_tag="IV142_001041"
     CDS             21166..21507
                     /locus_tag="IV142_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484712.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            21749..24538
                     /locus_tag="IV142_001042"
     CDS             21749..24538
                     /locus_tag="IV142_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAP8484713.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEEFRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNEIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            24528..25268
                     /locus_tag="IV142_001043"
     CDS             24528..25268
                     /locus_tag="IV142_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8484714.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYRNVTRKIECYYKAPSIDVVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            25265..28027
                     /locus_tag="IV142_001044"
     CDS             25265..28027
                     /locus_tag="IV142_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP8484715.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLAEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRLIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     SLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFTSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWRIYDILCNNGFTK
                     QSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLF
                     NAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYE
                     RPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYS
                     GYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIIN
                     WARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISS
                     IVIPPK"
     gene            28040..28948
                     /locus_tag="IV142_001045"
     CDS             28040..28948
                     /locus_tag="IV142_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAP8484716.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            28948..29568
                     /locus_tag="IV142_001046"
     CDS             28948..29568
                     /locus_tag="IV142_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484717.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            29572..30021
                     /locus_tag="IV142_001047"
     CDS             29572..30021
                     /locus_tag="IV142_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484718.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            30021..30515
                     /locus_tag="IV142_001048"
     CDS             30021..30515
                     /locus_tag="IV142_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484719.1"
                     /translation="MADIVQLKEDGVAKYLKTHVKAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            30529..30960
                     /locus_tag="IV142_001049"
     CDS             30529..30960
                     /locus_tag="IV142_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484720.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            30962..31099
                     /locus_tag="IV142_001050"
     CDS             30962..31099
                     /locus_tag="IV142_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP8484721.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            31136..31429
                     /locus_tag="IV142_001051"
     CDS             31136..31429
                     /locus_tag="IV142_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484722.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            31426..>31645
                     /locus_tag="IV142_001052"
     CDS             31426..>31645
                     /locus_tag="IV142_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP8484723.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGN"
BASE COUNT        11213 a         4663 c         6393 g         9376 t
ORIGIN      
        1 aaaaagcgcg cttatttgtg caacgtaatt tattatttct tcgttttaca catctataac
       61 aagtcctttc gtatatagat tctgtaccgt cttttttctt atatcttgct ttaatagttt
      121 gaccagctac tgatccctga cagtcagaac attttaataa acctttaaaa atgtaatcac
      181 gttttgcatt tgtttttaaa tttttaggta aggctttttg aacagcataa aaaagttttt
      241 tatcaataat aggaggacag taatttttat tatttcttaa ttcaccaatg tattttttgt
      301 tcgtcaacat atttttaaca ctttgataat ctctaacgat tccatactga gagcctaaat
      361 aataaacagt ttttcttaaa ctaccactag aaagaaagta gttgaatata tctatcacga
      421 taggagcatc atcatttatt actaatttct tattttctat agagtagcct aacggagttt
      481 tcccgcttac tacttcacct tgtgcaattt tatttgccat aacagctttg atgcgttctg
      541 aatcaatttg ggcttccaat tcagcaaaac tcattacttg tgcaatgaaa gttctgccat
      601 acgctgtagt tgtatcataa tattgttgag aaacagcatt ccaagaaaca ttgtgttttt
      661 ctaaaatttc ttgagtgttt aaataatgtc gtagatttct aaaccatcta tctaattttg
      721 taaataaaat tagatctatt ttattttctt ttacatcttg taatagtttt tgaaattcat
      781 cacgatttat tttttgacca gaaataccac cgtctacata ttcattaact actttcaaat
      841 ccttgtgttg ttcgatatat tcatagaggg tttctttttg ttctctaaga ctatctccat
      901 gtttggcttg ttgttcagtt gatactctca tatataatgc tactcgtttc atgttatctc
      961 ctttcgaatg tatgttctta tcgttgtaaa agaaaagccc gaaggctaat ctttttacta
     1021 cacatctgtt aattctaatg atcttctgta ttgttcagct tgagctagtt tagtaaaaga
     1081 tacagttccg ttataatttt ctaaaacaac tttttcaatt tcatcaagag atactttaaa
     1141 gaattctttt cgttgattaa ctttattgac ttcgtttttt ctaaaatgcg aatgtagttg
     1201 attttctaat gttggtgcat cttctgaaaa tatcattgca tgaacatcaa attcaaaagg
     1261 tacagaagcg ctactaagtt ccttaattct atccataggt tctaaccgtc tagtcattcc
     1321 tattttatag acatcttcac cgaaagatcc gatattactg atgacataaa catagccagc
     1381 tctagtatta agttctcttt gcaatacatc ttctttgaca gtttctagtt ctcttaattt
     1441 ctcttccagt tctttaattt tatcagcata gagttctttt tcaacatcag cactagcttt
     1501 ttgcatgtat gccattaact tagttatctc tgagttaaac tgtcgttctt ctttatcaat
     1561 gcgtttcttt tcacgttcta tttcacgtct taccttttct tcttcaatca tttgttcttt
     1621 tattgctttt tgctgttctt tttcatcgtt aactttaact atgtatgaat agttgagttc
     1681 taattcttga agttttaatt caagtaattc ttttttgaat tctgcttcat cgtttttaaa
     1741 aagcttgttt attttatcaa aagttcttat aattttttct ctagttgaat ctacgttaga
     1801 aatagtaagt ttattgagaa gtccagttat ttctgattct aatggtataa gcattttctt
     1861 tttaatatta ttagcttctc tattattcaa ataagaagga atattaattg caccgtctaa
     1921 aataaaatcc ttttctttaa tctttaataa agaaagtttg ttttttattt cggatgaaga
     1981 agtttcatct atattcggaa taattactga tttatagata gtgaccagag atgtgtcata
     2041 tttttgatta atttcatcgt ctaaccgctg gctttcagca ttaagaatat caattttgtt
     2101 ttctagcgtt gacaagtttt tattcaattt acttatgttt ttctcttttt cagatataaa
     2161 cgtttcccta gcatttaatt cttgagaata ttcctttacc ttactctcta aggcagaagt
     2221 tttatctttt aattttttag cttcagataa tttgctacta cccataatta ttaaaactat
     2281 tccaactaaa aacaatagta ctcccattca aaacgcctcg cttcttttat aaaataattg
     2341 tcaaaatcat ataaaccctt accgaagttg acggctccgg catgggaatt tttttaaaat
     2401 acaacttttc cgataattct aatctgttct ggcgatacaa ttctatcttc gtataagtcg
     2461 tttatagaac gtaaaatgat tgtttgattt tcgtaatctt tatatatctc tttacatgta
     2521 actccgtcac cacctaattc cacaatagca atttccccat cttctacgtc cggttgttct
     2581 ttaacgaata cgaattgtcc attcctgata acaggttcca ttgaatcgcc tataataggt
     2641 actgcgaagt cagcagcact tggaactctc tcaaattctt cctcttcgac aaccgtatca
     2701 ccgtaagtaa gttctgttgg attggcagcc gaagccgaat tgtgaacagt tgggattttt
     2761 tcttttttag gaaaatctag gacattttct tgtttttgtt cttctaatcg attacttgca
     2821 aattcatagg tagcaacttt attttcgtcg ttaagctgat tatatatagg aaggatatta
     2881 tatatctgat tagatataga taaatcaata ttcatcaaat catctatatc aacattgaat
     2941 atcttagcta tctcatttaa ggtttttaat tttggtgtat attttccttt ttcccattcg
     3001 cttattgagg atgaactttt tcttcctagt ttttcggcta gttcttgttg atcaatatta
     3061 tacttttctc taagaaactt tagattttca gcgaacatag taacacctcc ttgagttaat
     3121 tgtaacaccg tttcggaaaa aatgtaacag gtttcttaga aaatatttta gtgtttttaa
     3181 agaagtattt acgcaaaata gaaatcgatt tcaggaaaac tgaaatatgc acttgacttc
     3241 ggaaaaaccg aagtatataa tttagtcaga aagttaaaga gaggaggcga ataaaatatg
     3301 gataccgcga agaaagaaaa atatacattg agacaattaa gagctttaaa gggttattca
     3361 aaagaagaac tgtcaaggaa aagcaaagtg acatcaagaa caatatttat ttacgaaaac
     3421 gatgtagata aaatgaggaa cggaaagtac gccactctag aaaaaattgc taaggcattg
     3481 ggtgttagag tttccgatat ttttttagac cctgattcgg aaaaaccgaa atcaataaca
     3541 ggtgaaacaa aacaagtcag ctaggaggtg tgaatagtgg aagagaagaa tataaaagta
     3601 ccgactcttg aagaatatac aaaaattgtt gtcgaaacag acgagcagaa cccgaaaaca
     3661 atcgcagtta ttacggcgaa tgatattgaa tcatgcgaag gttttcgagt tcgaatgacc
     3721 cctagatatg attaatgttc tttatcacgt ggaggtatag gatcgtttcc aaaactatct
     3781 tttgattgta tttgtccatt ctttttttgg acaataaact ctgattgctg ttttttagaa
     3841 atctttttac catactcaac agcatctgat ttattatcaa atagttttgt atcttcggaa
     3901 ttaccttcac cacgtacagc ccatttgcca tttctaggag atacccattg atttttaccc
     3961 ataacaattc acctcgcttt caagttaatt ctattaagag gtgaaaagaa aacaaactga
     4021 tggaggtgag attgtgagta acaagccaaa aatcactttg aatggcacac ctaatcttca
     4081 aagattagca gatgaaatgg ctagaatacg ttctagacat tggggagtta ctgtggtggc
     4141 aaccataatt gaacaaaaag aaaagcccac acagccggca agcaagtagt gggcaccata
     4201 gaacaataat attatatcac acactcgcca cttccgaaaa gaggagttgc tgctctcctt
     4261 tcaagtttgc caaagtccac gtttcttcat tttggcaact cctcttcttg gaggtggtga
     4321 cggcgagaga cgaaggaggg atgacatgaa ggaagaatca aaaataggga ttttgaatga
     4381 gaacaattac acaagattaa ttattgaaac agaagaaggg gaaaaagtag cagaagttac
     4441 attgaccgat gcaactcctg ctgacggtta tcgtattcga ttaactccta aatatgatta
     4501 accttttggt gggtgtggat cgtttccgtg actatcacgg ctttgaattt ttccatctct
     4561 gccatgaata aatagctcag atccttgatt tttagaaatc tctctagcga tttttactgc
     4621 ttctgatttt gtattagtat gtgcagtcgc tttagaattt ccagcacctt tgacattcca
     4681 accaccattg gaattaggta caacatgttg attttttccc atagttttca cctccatttc
     4741 tatagaaata ttttatcaaa tagaaatgga gaaattcaat aaacgaaagg agggatcaaa
     4801 tgacattaca agagcatcaa cacttgatgc gaaaactaaa tcaggaatac cacgaggaaa
     4861 tgaaacctag attgctaggg gaacaatggc gtgaacgcca gaagaagtgg ttagaaataa
     4921 aaaagtgact cagccgtcca aagcaatgag tcacaaacca aaaattatac ctaaggagat
     4981 tttatcacat gaataagaaa attgaaaatc ttatcgaaga attgaaacgt gaatgccaaa
     5041 agcaaggtgt ttctattatt tgtacagccc aaaaagaagg ggaacttaaa agtctcgttt
     5101 atggtgagac aacagaaatt ttactttgtc ttgctatgca agaagaacat ttagatgaaa
     5161 atctcccatt gtcagcacat attatgcgta gaattgctgt ggatgcttac gaacaagcga
     5221 aaaatgaaga agaaaatcaa ccttcaaatc atacttttgt tattaataac aaggaagatt
     5281 tagctgatgt gatgactcgt attctcaagg gggaatttca atgatgaatg aaaaaacaat
     5341 tgatctactc attattttat ttttcacgtt agtgattgta gcgaaaagtt atgtaatctt
     5401 tggcttgtta ttcgcaatta ccatgcttgt gctttgcgtg ttagcaagca gaaaagaggt
     5461 taaacatgaa cgagttagca aatcttgaca actatttaac tgatcctgat tatgctaaac
     5521 caccatatga agcgccaatt gatgaggagg atgaagatga gtaaatctac cttagaaatg
     5581 agccatcaag aatggctcga agaccgcaaa agaggtattg gtggctcaga tgttgcaacc
     5641 gtacttggat taaacaaata caaatctcct tatcaattgt ggcttgagaa aacgggtcaa
     5701 attgaattga aagatttaga aagcgaacca gcttattggg gtaatgtttt agaagaagtg
     5761 gttgctaaag agtttcagga acgtacaggc aaaaaagtac gcagaagaaa ccaagtattt
     5821 gaacatccat tacatccatt tttaagagca aatattgatc gggaagtagt gggagaaaat
     5881 gccattctgg aatgtaaaac agccaatcaa tttctcggaa aagagtggga aggtgaagag
     5941 gtaccgctta gctatctctg tcaagttcag cattacatga acgttttaaa caaagactat
     6001 tgttatatcg ctgttttgat cggtggacaa aaattcattt ggaagcggat tgaacgagat
     6061 caagagctga tcgatacaat cactgaacaa ttagtagagt tttgggaaac gaacgttctt
     6121 ggaggtatcg agcctgtaat tgacggaagt ggagcgactg ctgacttctt aaaagaaaaa
     6181 tatgcagatg tagaagaaaa tcaaacagct ctaccatcac gttttgatga acttatcgag
     6241 caaaaaagag aactcaagcg gacgaaaaaa gaaattgaat catctattcg tcaagtagac
     6301 aacgagatca tcagtgagct aggtaaacgt gaagccagta tcggtatcac acaaagaaac
     6361 attatcagct ggaaacttgt tcgtacaaga cggatgaaca cgaagaaact agcagagaaa
     6421 tatccagatg tcgcaaatga tgaagagatt tataacgtta ctgaatcaag aaggctaacc
     6481 gaaaaggaga tcaaataata tggcaacaaa tgaatcgtta aaaaatcaat tggcagaaaa
     6541 gccacagaaa caagttgcac caggacagtt agggcttaaa gctctaatga atacaccaac
     6601 aatgagaaag aaatttgaag aagtacttca tgacaatgct aatgctttta tgtcgaatgt
     6661 tatgactctt gtatctaatg acagttatct tgcagatagt gaaccgatgt ctatcatgag
     6721 tggtgcgtta actgctgcaa cattaaatct tgggctagat aagaatttag gttatgcata
     6781 tttagttcca ttcaatagta aaaacaagca aacaggaaaa tgggaaaaga aagctcaatt
     6841 catgcttggc tataaaggat atatccaatt agcccaacga tcaggtaaat acaaagcatt
     6901 aaatgtgatc gaagtttacg aaggagaact aaaaagctgg aaccgactga cagaagagtt
     6961 tgagtttgat ccaaatggta gaacgtctga tgaagtcatt ggatatgttg gctatttcga
     7021 gttactgaat ggattcaaaa aaactgtcta ttggaccaaa caagaaatcg aagctcatcg
     7081 gattgctaac aataaagatc gcgataagac aaagttaagt ggtgtgtggg catctgatta
     7141 caatgcaatg gcacgaaaaa ctgttttgag aaatcttctt tctaaatggg gaatcttgtc
     7201 catcgaaatg caagaagcca ccacatcgga tgagagagtc caaagggttc aagaagacgg
     7261 cagcattatt gctgaaacag aagttgaaga agatattcct gaaagaaaag aagcagaggt
     7321 tatatctgaa gaaaatgaag atgtacaaac tggattattt gatgcatcta atccgccgtt
     7381 aaacaaataa tgagggagct ttctcccttg cttttctaga ggagaaatac gaatgaacac
     7441 aggatatgtc aaattacatc gaaaagtgat gaattcattc gtttggacca atccttatat
     7501 gtataaatta tggagcttgt gtttgatgaa agcgagtcat gagaatcgca agatactttt
     7561 taatggaaaa gagatacaag tgaacagcgg agaattcgtc acagggcgcg atgctatcac
     7621 atctgagatg aacgaaggcg tgaaacgtga acatcaagtg aacagcggtt ctgtatggag
     7681 atggctaaaa caatttgaaa aaaacggaat gttgaacatc aaatcaacta cgaaatacag
     7741 cgttatatca ataaaaaact ggtctttgta ccaaagcagt gaacaacaaa tgaacatcaa
     7801 acgcacaaca agtgaacaac aagtgcacac aaacaagaat gaaaagaatt ataagaatga
     7861 aaagaataat aaaacatcat cgtcgcaacc acgcaaaaag cgtgtttacg acaccgactc
     7921 agtttactac attctcgcgg aggagttatt caaacagatt tgtcagaatc aggaaatcaa
     7981 aaagccgaat ctgcaaagtt gggcagataa cattcggaaa atgatcgaga tcgacaagcg
     8041 gaccgaaaac caggtacggg gaatgattga atggagtcag cacaatgtgt tttgggcatc
     8101 gaatatttta tctgctaaaa aattgcgaga aaaatacgac acaatggcag cgcaagcaaa
     8161 tcgtgattat aaaacaaaac aaactaaaac gcttgaatac gagaaattta gcacagatga
     8221 gttgcctatt tgagaggagg cgtaggcatg gaaactgttg gggaaatcat ggaaaagctg
     8281 atacagaaag tgcttgtcca acgtggcgaa tgtcctgaat gcggacagcc tttgtatgga
     8341 tggcgtacga agaaccctga tggctcagaa cgttgtaagc caacctgtat gcaatgtggg
     8401 tataaagcat tgcgagttca ggaagactta caaacagaac gaatttacaa cgagagcctg
     8461 aaagcaagag caatcaattt tttcaaaggt ggttctgttg tgcctaatca agcgttgttt
     8521 gattgcacat tgcagaatta tcaaattgtc gatcaagaaa caagacaagc ggttgaagta
     8581 accaaacgct ttgttaattc agtcttgtta ggaaatccaa gtcaccttgt tttaactgga
     8641 aaacaaggaa caggtaaaag tcacctagcg atggcagcgg cttgggaagt cttgaagcga
     8701 tcaaactacg ataaaaaaat cttatttatc gggttacagg aaatgctgga tcaaatcaaa
     8761 ttttcgtaca acaatcctga actcagaaaa acgattgagg gatcgttgat tgcagatatc
     8821 aaaacagcgg atttagtcat cattgatgat atcggttcag aactaggaaa agatgcatca
     8881 gatagtcgag cgtttggcat aaacacgcta aattcgttct tggatgcacg acagaaccta
     8941 gcaacgatta tcacaacgaa cttgcttggt gaagaactga aaaaagctta cggtacgaga
     9001 acaatatcaa gaatgtttgt caactctgat ggatttacga tggtattttc tcaaacagca
     9061 gacaagcgca taaaaccagt caaaggagcg atcgcatgaa tttgactaaa caagaaaaaa
     9121 gaatctgtcg tcagataatg ttgcaaatta taggtgatcg tgacatggac gaattatctc
     9181 aacaagagag tcgaatggtt cggaagataa gtaagctaat cggtgcagat acgatggatc
     9241 gatccaagcc attacccaaa ctgaattttg aatcatttac gtttgagaaa tatagccatc
     9301 tgagagaggt aggctatagt gtaaatgcta ttcgaaaagc acttggggta ggaagtagtg
     9361 tatgggcaaa atggcggaaa gaaaatgtaa atgaggtaaa tcaaaatgat taagctaata
     9421 ggcaaagaga cgctcaaggt ttactatgta gcggaaacaa aggcagaagt cagccgttgg
     9481 ctattgaaaa tgtttaataa gactagagcc aatgcttcag taagtcagaa agcaagtgta
     9541 tcagaaacag gcatgcctga agcaatgtgg atttcaagga ggaactgaga tggcaatgat
     9601 accgaagttt agagcgtggg atagagaaaa agacagaatg aaggcagtag ctttggttga
     9661 atttgctgag gcgtataaat caggagaact catttcacgt acaaaataaa aaaagtcgga
     9721 ttcctccgac tatgaataat atttccgaca taagtattat atcataattg ggggaatcag
     9781 aggatggtac ttttcgatgt aaagaaatat gagacaccaa gcgcgaaaga tgttgatatg
     9841 gaacgcacaa aacataatgt cgctgtgttt ctttcagcat atctatcagc tagatgtaga
     9901 gtagggcaac ctcgagaacc aaaagtgaca gcatcttatt ccttggttcc accttctaca
     9961 gctgatcata tatttgaagc agaaagaatg ctgatcgata aagaagaagc acaggaagaa
    10021 tttgagtatc tgcataaatt gtttgttcga ggctattcag cgatacagca tccgcataag
    10081 cctgatgtga ctgaaaggcg caagaagata ttctatgatc gttatatcaa tggtctgccc
    10141 atttatgtaa ctgctcaaag gaataatact agcgaagaat cggttaaagt agaatcaaac
    10201 agaattatca tccaatttgc ttcatcgtta gaactggttg ctttcaagta gccagttttt
    10261 acacttttta taccctttta ttaccaattt ggtttccatt ttataccttt tttataccaa
    10321 tcacttacct attcaacgtt gtattatgat agtgtcgaaa gattaggaaa caggatcgac
    10381 aaaataaaat gtaagggagg aaatctccct catcgtttaa ttaagcttcg ataggcagca
    10441 gcggaaatat taagaataag gatgtgaatt ttaactcctt ctaaattgtt cttattatct
    10501 atcatccgtt gctgtctatt gttattatgt cactgtggcg gaaagggtag acgcttaaaa
    10561 ataaggtcaa tacgtcgagg gatagcctta acgttttatg atttgaccat gcaaggttcg
    10621 attccttgcc agcgacttag acgaaaggta gcttaaatat taaccacatc aacatcgtca
    10681 gcgagttgat aaaggtaact agcagactct aggatttaag ccagtcttac cgattagtct
    10741 gttatagtag caagcttgct agaggtagct cctctggttg acgtgcagct cagttggtag
    10801 agcgcctgac ttttaatcag gaggtcgccg gttcgaatcc ggtcacgtta ataaagaacc
    10861 tacggaaaca atcaatctta tcggatgccg attggttggc tgaattaagc agtgtgtctg
    10921 tgaatcagtt aataggcata aaatactagc acagacgtgt gccactctca ggtgtaggtt
    10981 aggagagaaa cattagcaac cgagggttgg aaatgggcgc tcaaagtaca cgagcaaggc
    11041 gaggtcgata gtaatcgatg gaatcggtgt aggttgctat tacataattg attgggttag
    11101 attgagtttt gggattcggt acaaatgaat cgtcaaatga ctcaagcaca ggatcggaaa
    11161 cgtccctgcc tgtgcattac atattagatc actcgttgag tggtcttttt attttagaaa
    11221 agagaggatt ttgaaaatga atcatgagaa gtttatcgaa aaatgcaagg ctattgtacg
    11281 tgaaagaatt gagaatgaga ttgctgacct aagtggagca gtacctgagt ttagtgtttt
    11341 catagtctgg tcatgcaaaa cactacagaa tagcaaagca ttagtcagcg ctagcttaaa
    11401 aggagcaccg tattttgaaa ttacgttgaa cggggacaaa ggtgaaatct atgtagatac
    11461 ttatctcaaa aaatcaaatg aatgtatcaa agtctagcag gtgctaggct ttttctttac
    11521 ataaaggagg aatgatctca tgaaacaata taccgctaaa gatttcgagg aaatgaagcg
    11581 attaaagaag gactatgaag aagttgatat ggagctaact gttggagtca ttcaacgaag
    11641 actgcgggtc ggattagaga cagcaaaggc tatttacaat gatctaaatg ctattgaaga
    11701 gaagaatggc taatgaggaa ctactggtat atatcgctaa ctaatgaata tcctcgaacc
    11761 attgatgatt gttcagtgcg tgttgtgcgt tctgtacaaa tcaaagggaa gtactctatt
    11821 gtcgaaatgg ggagagaagc agaaccatgt gagattgatg cgtgcaagct cgtttattgc
    11881 ggtcatggtt tctatgatga accaaacatt caaaataata ttaacaagaa tttgagggat
    11941 tagaatgcaa gaaatggctt aaaaatctcc aatcaggaca aatcaagtta gacaaagttt
    12001 ccgatttaaa gatattaatt gaagcagatc taatgttgaa agatattgaa aattagaaaa
    12061 caaaactcaa cctaagaaga ttgcgaggtg gtgtgtattg aatggcaaga caacgtgatc
    12121 caagacgtga tgaagccaaa agaatttggt tagaatccaa cggagaaaag cagttaaagg
    12181 aaattgcatc tgaattaaat gtttcagatt ctcaggttag aaaatggaaa tcgcaagaca
    12241 aatggagcgc tgaattgaaa agtaacgtta ccaatggcaa aagtaacgtt actaatcaag
    12301 gtggcgctcc tattggtaat caaaatgcta aaggtaacaa aggaaatagc cgagcttctc
    12361 cgccagtggg taataaaaac gctttgaaaa caggcgagta tgaaaccata ttttttgaga
    12421 cactaagtga tgaagagaag gacatctatt ctagtctgaa tgataatcct tcttttgttt
    12481 tgtctgaaga aatacgtcta cttaagataa gacaatttcg tatgatgaag agaatccaac
    12541 aagctgaagc tggactaaat gatgaagaag tcgaacgatt gcagcagcta agaaagatta
    12601 aaaatccgat tgaaaaaaat ggtaaaaagc tagaaatcaa gcgtgaggtt atgcaagatg
    12661 tgcagattag cagaaaaaaa catcgcaaaa ttgatgatat tctttcaatt gaagattcat
    12721 tgactcggat tagcaaccag ttagctaaag ccatcaagca aatgaatgaa ctttatatga
    12781 atgaatacag aactgattta attaaagctc agactgataa gatccaagct gagacaaatg
    12841 aaattggcgg aaataattca ggtgaagaaa tagaagaatg gaaacaggca gttttaaatg
    12901 ccgcaaacaa acgggcggtg aaagaaaatg aataatgaat ttattccttt tgctgatatt
    12961 ggttctgcca ttgattatta ctatgataaa ccagtagctt tttgccaaga tattttgcat
    13021 ttgaatcctg atgaatggca agaaaatgtt ttaaatgatt tagctgaatt ttcaaaggtt
    13081 tctgttcgtt ctggtcaagg agttggaaaa acagcattag aagcaggagc aatactttgg
    13141 ttcttaacgt gtcgacccta cgctaaagta atagcaacag ctccgacaat gaagcaactt
    13201 tacgatgtac tttgggcaga ggtagctaaa tggttaaatg atagcttgat caaaaactta
    13261 ctgaagtgga caaagaccaa aatttatatg gttggtgatt cagagcgttg gtttgctacg
    13321 gctagaacag cgactaaacc agaaaatatg caaggttttc acgaggacca tatgttgatt
    13381 gtggtagatg aagcttctgg tgtgtctgat ccaattatgg aagctattct tggtacgcta
    13441 tcaggttttg ataataagct gttgatgtgt ggaaacccca ataatattga aggtgttttt
    13501 tacgattccc acaattcaga ccgtgataaa tacagagttc ataaagtatc aagctatgat
    13561 agtaaacgta caaacaaaga caatatagaa atgattctta aaaaatatgg aaaagaaagt
    13621 gatgttgctc gtgtccgtat ttttggagaa tttcccaaag gtgcgttgga ttcatttatc
    13681 agtcttgaaa cggttgaatt ggctacagaa aaacaaatta gtgattcttt agtcaataaa
    13741 acaacggttg ctcatattgg tgttgacgta gctcgatatg gtgatgattc tacgattctc
    13801 tttcctagaa ttgctaccag ggcattggag tatgagaagt attcaaaacg tagcaccatg
    13861 gaaacaacag gatatgtcat caacatggcc aagaatctaa tgagtcaata tccgagtatt
    13921 gataaagtga tgattaaagt cgatgacact ggtgtcggag gtggtgtaac cgaccgccta
    13981 gaagaactta tagaagacaa acattatcct tttgaggtgt ttggagtgaa taacggttca
    14041 acatcagaag acgattttta cgataattta ggtactcaac tatggggaaa catcaaggaa
    14101 atgttagaag aaaatatgac agcaaatctt aacggagaac agcctgttat tgaattgcct
    14161 tctgatagtt cgttaatcaa agaattaagt actcgcaaat tcaaaatgac aagtagaagt
    14221 cgtatacgtt tagaaagtaa agatgatatg aaaaagcgaa atattggtag tcccgatatt
    14281 gctgacgcac tggctttagc gttttatgag ccaccaagtc actatcaatt tattcaattt
    14341 taggaggtga gcttttgatt agtacagtat tatctttaga gagatataag aagctacgtg
    14401 ttaaatatgc aacacaaatt gaagatggaa tgtttgatcc gaatggtttt atagaagata
    14461 tgaaaccatt ttttgctgat cgcgaaagaa aatatctagc ttatactagc gaaaagaatg
    14521 aaatagataa tagaccaaag cctaacactg atattgtaaa agttaataat aaacttcatg
    14581 ctggtatgta ttcaattgtc gtagaccaag cagtcaatca ttttactggt atacctatca
    14641 aatgggatta cgatgtatcg gaacaaaaaa gaacactcat tcaaagatta aaagataaat
    14701 tcttaaaaaa tgatatagaa cttccgacag ttccagaagc ttttaacaaa ttaacaagca
    14761 atcttgattc catgagattc gcaatgcttg attctgaaac tgcaactttt caaggagctt
    14821 gtggagtagc tttccgattg ttagagcctg tggaagaaga tgatggttgg aagttaagag
    14881 caagtaatat tgaaccttgg agagcagaaa gatatggaaa tgccggaatc tatatcaaag
    14941 aaaagtatga ctcttaccag aaaaaatttt ttcaagaaat gaaagttatg acaagaaata
    15001 aaattctgac atatgcttgc tatggtgatt tgaattttgt tacaagcgga acgttcaaaa
    15061 aaattgatga gaccgataac cctttgggga cgattacctt ggcagaattt aaaaataata
    15121 cgaatcgtta ttgtgatttt gaagtagctg aggaaattgg agatgcaatt gatcgtgctt
    15181 tgtctgatca acaaaacgaa atcgaacaat ttaagcttgc gtacatgctt gttactggca
    15241 caacaatgag caaaggcact gctaaagaaa tgatgaatca gctagggatt atcaacttaa
    15301 aagaccctac tgcaaaagct gaatatgtca caaaaaattt agcaaaagat ttcaatgaat
    15361 atcatatgga tctattgaaa aaacagtttt acaccatttg taaagcaatc gattttaacg
    15421 atgaggtgtt taaatcaaac agttctggag aagctcgcaa gtggcaaatc attagcctag
    15481 aagcgaaaac aaatactaaa gagcaatatt ttagagaagg attgaaagaa tgcgcagaaa
    15541 caattgctgc ttttcttaag tttcatgaca aagtagaaat tgagcctgaa aaaattattt
    15601 ttactttctc tagatctttg ccaactgatt taagttattt agctgaagct ttacctaaac
    15661 ttgcaccata cgtatccaaa cgaactattc agagccaaat tccatttgta acagatgttg
    15721 attatgaaaa tgaaatgatg gaattggaaa gtggaagtgc ttatccagat agtgaataca
    15781 attttggcgg aggtggcaat agtgacgata gaaacgaagt attggaccaa acgtcgagaa
    15841 ctggaggatc aagcaaggct caaacaagaa aatcagacac ttaaaaaatt aactagtgta
    15901 tttcctgaag cactgaaaga gatacaagca aaactattat cacaagctga cttacacaat
    15961 atcacttatc cagaaatgat ggagttttat agtacaagta atcagaaaaa atatcgtgaa
    16021 tatgtggaaa aaaattataa gtcattaaaa atgtatgatg caaaatacaa agagtttatc
    16081 gatgaatttt ttccaccatt tgactatgca aaagtcaatc gcttattaca aatacgatca
    16141 gatgtattta aaattcttgc agaatatgcg atggatgcag atgtgaatca atatttttct
    16201 gatcgcttag aggaaattct tcaaagaaca tattcttcta atgctaatgt ttttgttcaa
    16261 cttttaaacg ttgatatacc aaattattta ccagaaaatg aaattaaaca ttatttgaac
    16321 tatccatggt caggaaagac attttcaaga agactttggg gaaatatttc atcgcttgaa
    16381 cagaaactct ctaatgctat tgtaaaaagt gttgctagtg gagaaggcgt tatacacgca
    16441 ttaaacacca tgagattaga ctcagaaatt tgtgacatgt ttaagttaga agaatcaaag
    16501 tataacaaag cgatagaaaa tctcgttcga acggagtatg caaaatttgc acaagatggt
    16561 attgaaaaat catatttaga aacaggtatt gaggaataca acgtattgac tgcaaaagat
    16621 gagagagttt gccggatttg tggaggaaag gcaagtaaga atccctataa actgaaagat
    16681 gctgtcatag gtgaaaatcg agcacctttc catagtcgtt gtagatgtac ggatgttcct
    16741 aatttaccaa aattaggaaa ggatattgat gaagaatatg accgtttatt tggcgattta
    16801 ttagatgagt ttgcacatga ttcttttgga attaatttga aacggaggaa gtagaatgaa
    16861 agattttttt gaagcagtac taacaattaa tgtaaatgct gatattgcag aagcctacaa
    16921 aacagctatt gagtctgaga accatcctaa tggcttgaga gaccattgga atggcaatta
    16981 tgcctacgtg gttattggcg atcaaactgt taattatcaa gataatactc cagttgataa
    17041 gaataccgtt aatttaacga ttcaattatt atctcattca ttaccaaatt taaaagaaac
    17101 agttgattgg tatgaaaaga tgggatgtat tgttgttaga actgactaca aagaaggaaa
    17161 gtctagtaat taggcttttt tattttgtcc gaaatgacgt taaactagcg caatactggg
    17221 cttggttgaa tggtggggcg caactattaa aactcaaagc aatgcggggc gtgaaaacga
    17281 atcgtggggc gaaaggagaa tgagcatgaa acacaaatca ttaatgccaa tgaatttgca
    17341 atactttgcc gaaggtgacg atcaaaagtt ttcttttgat gacttcaaat cttttgtgga
    17401 atccaatgaa gaagctcaaa aatttattca gtcacaatct caaagtgttg ccgacaaaca
    17461 attggaagct tggaaacaaa ataacttaga taaaattaaa caggatacca tcaaggaata
    17521 tgaggaatct aagaaaaaca agtcacctga gcaaattcaa ctggaaaaac tacaagctga
    17581 atttgaagca gaaaaagcgt tgcgtgtgac aagtgataat aaagcatttg ttgcagaaca
    17641 aattgctgga ttagaactag atggagagtt aaaagagtct atttctcaat ttatgctaaa
    17701 taatcttgtt agttcggata cagatttcac taagaatgct gttgaaggtt ttacaagtgt
    17761 cttgaatgca attaatgaga aacatgcaga tgcattaaaa gaactacaaa tgaagtctgc
    17821 atttggtgga actcaacaat cgaataacca ggttcagcag aacaatgaaa cattaacaaa
    17881 tccagaagaa caattaggac aaattcttca acaatttaac taggagagtg aaaaattatg
    17941 aaaaaaacat ctttaaataa tttagagtat ttggatattt caccagcgat taatgctatg
    18001 caagtaccaa atacaccttt tttaagctat ttatttggtg ctggaaaaac agagccagca
    18061 aactcgacag aaattaaatg gcgtgaatat gatatcaaca acgatgattc ttctgaaaaa
    18121 cttgagggcg gagaatatcc agatgctgaa tcaggtcgaa cttggtttaa caactatact
    18181 gaaattttta gaaaatcaac ctctgtatct ggtacattag atgctattaa tgtgaatgga
    18241 gtcggaaatg aattaactaa tcaagtagca ctacgtggta tggaaatgaa aattgatttg
    18301 aaccgaaaat tgattactgg tgtaaaagct gatgaaaatg gttctaaagg tcgtcgaatg
    18361 aatgggattt tgaacttgat caattcagca aataaggcag aaacagccac tgcgggtgca
    18421 gtaacaagaa aagatatcga tactttattt aaattgatgt atgaaaaagg ttatatggga
    18481 gaaaaactat gcttgatttc tccagatatg caggagttaa tgactgatga gttagatgga
    18541 aaatcaacaa aaattgttca gttcggggaa agagtaactt ttggattgca aattggaaat
    18601 atcgtgtcta attacggtac aggtattgct ctactagaac catcattgcc aaaaggaaca
    18661 attgccgcaa tcgatactaa ttatgtgaaa ctacgtccat tacgtgaatg gagagcagaa
    18721 gaacttgcaa aaacaacgga ttctagacgt atcggtcttg taggtgaata ctctcttgaa
    18781 tacaatgctt caaactctgg ggcaatttta aatttaaagt ctgaataaaa gggagttagt
    18841 actccctttt ttgataggag gaattacagt ggtaaaaaaa gatgaaacta aaaaagatga
    18901 agtcgtgaaa tatagagtag gtaaaactaa aaattttgtt gggtttgttc atcctaaaac
    18961 tcgtagattt atcacagcag attcaaataa cgaatttatc atttctatag atgataaaga
    19021 agcaattgca attttggaag atgcaattga tgttaatgaa atttaggaag tgatctgatg
    19081 gatgaatcgc taaaaacgga aatcattgag tctacaaaag aagattttcc agatttgagt
    19141 gaagaacgca taactaattt attagaaata attttgctag aaattgaatc atacaacact
    19201 tgtaaaaatg atatttcatg ggaaaagtta aaaagcgtga ttagtgaagt gttgtataaa
    19261 ataataaaaa atgaatcaga aaaaacagta tcttcagtta gacgtggcga tacaacgatt
    19321 agttatgctt caacaacaaa tgatgttagt gagttacttc taggctatgg tgatttgata
    19381 cgaagagtta ttggttgtgg aggattggag tttttttaat gaatgaagca gatattttag
    19441 aaactactta tgaagatagt tgtattattg aaagactgac ggacattgaa gattctaata
    19501 caaatattac tattcaagat tacaaaaaag tatatgataa tcctatttct tgcgctcttt
    19561 cacaaggtca aatcgatgga ctagcagtca tagaagatgg agagatggta aatgtttcaa
    19621 ctgacacata taaattattt gttcatccta agattaaact aaaaaaagga gatcgaataa
    19681 caataactca aaaggccagt ggcttaattt tttctctatt tgctactaag cctttttact
    19741 atcctagtca ttgtgaagtg aatttgatag gaagtgagaa aaatgggtaa tctcaaatat
    19801 gaatcaaatg cagaaaagat cattgagaat tttaaaaata tgactgtaat tgctcaaaaa
    19861 gaaggaatat ccttcgttaa cgattcaatg aataaagtcg ttagtttagc taaaccatta
    19921 actcctgtaa aatcaggtaa tttaaggcga ggatatcgtg tagtaaaagc tagaaaacta
    19981 tcaagcggtc gaattgttgg agcagttata aacaatgaac attattttaa atatgtagaa
    20041 gaaggtcata ggactaaaaa tggtggcttt gtcaaaggga aattcatgtt aactcgtgca
    20101 acgaatcttg caaatatgtc ttatattcct cgaagattta aacaaatggc aataaaaatc
    20161 gttaagaaag gaaagtaaca tgaaagatga aatcattgct gcaatcagta gcaaattaaa
    20221 agaaatctat cctgatggga caatatatct tgattcagtt atgcagtcaa ctaaagattt
    20281 ttatttcgtt ttatcagtaa tggaatctgg aactgaaaat gtaggaattg atattcaaaa
    20341 tgtttctttc ttaattgata ttgcattgat tgataataaa cctaatagaa atttaattaa
    20401 tgaattagtc tcacgttgtg ggactttttt taatacgatt acaatcgatg agcaaatatt
    20461 atttccaaaa gcctatttac ctgatgaagc agatggtgtt caacatattc gttttacatt
    20521 agaatttcca caatatattg aatggagtga aagataaatg ggagaaaaaa gaagtaaaac
    20581 cggaattatt tctgtcgaaa aacctacttg gtttcctttg gaagatgaga cagggaattt
    20641 tccggtatat ggtactgcgt tgccaatggg aacagcagta agtatcaaac caacagctaa
    20701 ttatgaaaca acacaagatt atggggattc agtggttcaa gatcaattta cagcttttgg
    20761 tggtgcagaa gttgaactgg aagcaaatgg atatactcat aaagttttat ctacaattac
    20821 tggagggaaa attgttaaag gtggtgcctt gcgttctgga gaagatattg cgcaagatgg
    20881 tgcgtttgca tatagacgta aaaaatcgaa tgggaaatac cgttatacgg tcttttataa
    20941 aggacagttt gctctagatt cagatgaaac atcaacgatt gaaggaagta aagtaagttt
    21001 tactcatcca acttggaagg gttcatttgt agatgtacca ggacttggat atatgtattc
    21061 agttgatgaa gatgatgaag gcgtggataa agcaatgatc ggaaattggt ttacaaaagt
    21121 agcaattcca attgaagaag cagaactttc aggaggtaca gaataatgtc aaaatatcaa
    21181 acaacgatta aattaacgaa aaaagatgaa gaaggtaaat atgagcaagt acaatttaaa
    21241 tctgctgaat ttttaccagg aacagttgta gaagatgctg caggagttat ggaagaaatg
    21301 caaacagcga ctgataaaca atcagttaaa aaagctttaa gtcgtgctta ttcatttatt
    21361 gcagatactt tatttgaggg acaatttact ggtgaagatt attgcaaagg aattgatgct
    21421 cgtgagattg cctcattgac aggaaaatta ttgaagtctg ttactgcagg ttttgatgaa
    21481 acttatacag agacgaaaaa aaagtaagtg aggcgctcaa gtcaccttca tttaagtatt
    21541 cgattactta ccgagaatta gatataaaga cgcaattact cgaagcaggt tggacgttac
    21601 cagaaattga acgtactgac ttggatgagt taatgcgtct ttttgctttt agagatgcgg
    21661 taaaagagca tgaagatgtt gagtactatg ataatttcac tcaattttag gaggtgatac
    21721 tttgaataac gatgacctaa ttctgaagat gatactagat gaatctggtt ttactgccgg
    21781 tatgaataat gctgtcaaaa agttgaactc ttttgatgaa acaatcgaaa gaaaaagtag
    21841 aaatagtggc agttcattag gtagtatttg gaaaatattt gctggaagtt ttctagctag
    21901 cggagtaact agaatcgtag gagctggctt tgatctaatc aagggttcca taagtggagc
    21961 ggttgatcga gtagatacga tgaataacgc tctacgaaat ttccaaaata tgggatttag
    22021 taattcagaa attatgaaga atatcggaaa gaatgggctt ttatctcaag gtattcaagg
    22081 acttcctact gctttgaatg atgcgataag tcatgttcaa cttcttgctt cttctacagg
    22141 tgatttaact cgttcaactc aaatatttaa agctttaaat gatggaattc ttggtttcgg
    22201 tggttcaact gatcaagtta atgaagctgt tattcaatta tctcaaagtt tctcaaacgg
    22261 aaaagtagac gcacaaactt ggaactcaat gatcaacgct caacttggac ctactctttc
    22321 tgctattgct aaaaagatgg gaattacaat gggagatctg aaagaaggtt tgtctcaagg
    22381 taagatttct gttgaagaat tccaaaatca attaatagaa atggatacca aaggtggcgg
    22441 aggacttaaa tcattaagtc aaatcgctaa agattctact aagggaatta aaacctctat
    22501 acaaaatgca aaaacagctg ttacacgtgg tgttggtgag gtaatagaag gattaaataa
    22561 agcattagta gattcagatt taggtggatt taagggaatt attgataaag tgggcagttc
    22621 aatggaatcg ttcttaaaag taattgctgc aaatattcct acagcagtat catttttaag
    22681 taatttattt aacgaaattc aaaaatttgg ttctgctttg aaattcatga tgccctttct
    22741 tgttcctgca gctaccgctt ttggagcgtt catgtttcaa cttaaaggta taccagcaat
    22801 aataaaaagc ttcaataatt ttaagaatgc cataattggc gttggaaatt cattaaagat
    22861 tatgggggca atagctgctg ctaattcatt tgttttaatt attggtgcag tcgttggtgc
    22921 aatagctgtt ttcggttact ttatggcaac caatgaagag tttagaaaca aggttatatc
    22981 tatttggaat gatgtgaaag attccgtaat tggtgtatta aaaaatataa aggactgggg
    23041 aattgatact tggaattctg ctaaagaaat ggcatcaaat gcagttgagg gtgtcaaaga
    23101 tgcttggtca ggaacaaagg aatggttttc taacacctgg aaaggtatta aggatggggc
    23161 cacaggttta tttgataaaa cagtagagac ttcaagaaat gcagtcgata gtgtaaaaaa
    23221 tgcatggtct aatatgaaga aatggttttc tgatacttgg caaagtataa aagattcagc
    23281 cagagaaaaa tggaatgaaa ttaaaggttc tattatgtcg gttgctggtc cattgattac
    23341 aggtataaag aatgcatttt tacatgttac tttttatcta gagaccttat ggaataattt
    23401 agttgagatt ggtaaaaacg tctttgagat tttaaaaaat gttatacttg cgccggtttt
    23461 gtttattacc tccatgattt ctggtggatg ggaagaaaca aaaaataata tgattgctgt
    23521 ttggaataat attaaagaaa gtgccataaa tatttgggaa tctataaaga atatatttgt
    23581 aagttatttt acaaatattt attttgctgc actcaatatt tggacagggt tcaagcttac
    23641 attgataaat atttggaatg aagtggtaaa tcaagctaaa tcgatttggt taaacataaa
    23701 gtattttttt attaatcttt ggattgatat aaaatatttt gcaattcaga aatggattga
    23761 attgaagttt tctattattc aaacttggat cgatttaaaa tataatgcca ttactacttg
    23821 gaataatatt aaacagtttt tcaaagatac ttggaaaaat attaaagata cggcatacaa
    23881 tacgtggatt tctataaaaa ataccatgat taatacatgg aataacatta aggactcttt
    23941 ctggaatatt gttactggaa ttgttaattc tgctgaaaat gcatggacaa atctaaaaaa
    24001 tggtgtttca aatgctatta atcgggtaaa agaaatcttt aattcgttaa gggaagtcaa
    24061 tttatttgaa attggcaaga acatcattga tggacttata aatggtgtaa aagaaaagtg
    24121 gaatgctttg aaagatacta tcaaaggtat tgcaggtaat atcaaaaatt ggattaaagg
    24181 agctttagat attcattctc catcaagatg gatgcgtgat atggttggta aaaatattgt
    24241 tcaaggtatt atcgttggta ttgataaaga gcaaagtaag ttagatcaaa ccatgacaaa
    24301 tttagtaaaa acaccgtctg ttcaacctgt agtaaatgga ataagtcacc taccaattac
    24361 acaaacaaag caaaatatat ctgctagtga aacaaaagaa attcatttac atttaaatgt
    24421 ttatggagat ttacctgatt caatggttcg gaagattacc acaaaaatta aaacagaatt
    24481 gacaagacaa atgaaacgag atgctggtgc agtaggagga acattatatg caacttaaga
    24541 gaggacaatt ttttattaat caacattatt cttctgaatt taatgtgtat attcaaaaca
    24601 ggcctgcttc tgtttcagct agtcgagtaa ttgaattgag agagcgagaa ggaaatgatt
    24661 ctatcattat tgataaaggc tattacagaa atgtgactag aaagattgag tgttactata
    24721 aagcaccttc aattgatgtg gtgcaggagt gggaagatcg aatcactgaa tggttagata
    24781 tgtcgtctta tagtgatttt attctttact atgatgaaca atatatttat caagctgtag
    24841 taatagaagg acctgaattt aaaggaacaa gaaaaacagg aaatattgtc ccttttgaat
    24901 ttacagtgag cattcgacca tttaaggaaa attacaatgg tagatttact attcgacaaa
    24961 cagaaacttt tgaaatttat aacccagaga agtattcttc aaaaccgctt attaaattga
    25021 gtggttctgg agatgcttct ttttatatta ataaggacaa atatgatttg aaatcgttgg
    25081 acagagaatt atttatagat tctaaattag aagaggctta ccgaaaatta gatggtaatt
    25141 tagaacatca ggatcaagtc actttatttt tagattttcc atttttatat cctggaaaaa
    25201 atgaaattaa atggacgaaa aatattcatt catttgaaat aatgcctagg tggtggagaa
    25261 aagtatgaaa ccaagaatat atagtcctac tgaaacagat ttctcaacga atggcttagg
    25321 aattttaaag gattgtacaa gatgtgaaat atatgaagta gctaatggaa aatatgaatt
    25381 agagttggat tatcctttag gaactagatt tgatgaatat tttgaaaatg actatcaaat
    25441 aaaagcaaag ccaaacgatc aagaagagta tcatatcttt tttattgatg ataaagatat
    25501 agatactttt ttaaatacag taactattta tgctcagagt cgtacaaatc gacttggaag
    25561 acgggtagtc actcttgcgg aagtagactc taaaactggt caagaagcga tatcaattat
    25621 tgaaaccaaa atggataaaa aatctgacat acgactttat tctgatatta cggccgtttc
    25681 tagcacaatc tttgaagcga gaaatgtttt aaattgtatt gctggtgaac aaggatcatt
    25741 gcttcaatat tggggtggag aaattaaacg tgaaccattt aagctttctt tgttaaagcg
    25801 aagaggtcgc aataatgttg gaacgattcg gtatggaaaa gatatgtctg gtttaaaggt
    25861 caaattagat tggacaggta taaaaacaag acttattcca tatgctgatc ctcaaagtga
    25921 ggtaggtacg actaatcgaa tttatggttc gccagtagat agtgcctata ttaataacta
    25981 tcctgatgtg tatacagagc atgttcagtt tacagaagaa caaggagtaa aagacattaa
    26041 gagcttaaat aaaatagcta aaaattactt taaaacgatc aatcctggct gtgataagcc
    26101 taaagtttct attacggttg aatttgataa gttgactgat agtgaagaag cgaaagaatt
    26161 tgcgaagatt agaaattatg gtctgtttga tacatttaaa atatatcata aaaaatataa
    26221 tatttatctt gaatcaaagg ttagtggact acaatacgat tctttatctg aaaaaacttt
    26281 gaaattagaa gcaggagaca ttcaagttgc tttttatcag caacaagctg taactattca
    26341 ggataaatta aaagattatg caacgaataa ttatatgagt gattttaatg attatgtttc
    26401 ttcaatgatt acaggtcaag gcagtgcagg aggatatgta agtttatggc caaaagaaaa
    26461 accttccaac atctttatta tggataatcc agatttagaa aaggcaagag aagtactaag
    26521 aatgaataaa aatgggattg ctttttctaa gaaaggatgg cgggggcctt ttacttcagc
    26581 ttggacatta gatagtatat ttaatgccaa ttttatacaa actggattaa tcaaagcaga
    26641 tatctttcaa aattctttta acaagactgg agatgtattg aaattagtta atggactact
    26701 ccaaatttgg aacaacaaaa agaaaataat ggagttaact aaaaaaggaa tggaattttg
    26761 gaattctaag ggttcaattg gaacgattgg aacaactgat tctgctggta atccttttcc
    26821 tggggcttct actccaaccc ctattgaaga taattcttta gttattcgta caaatggaga
    26881 cggcaaatat attttgattt ctcctaaagt aggaaaagga ttagttttat taggaaatgg
    26941 taaagcaatt tattttggag acttagatgt acaaggaaaa cttacagtta aaggaaaaga
    27001 aataattcct ggacaaggtg gtggtccatc gggaggtgga gaatctactg gtgggtatcc
    27061 tagtgaatta aaaacagatg cagaaaagag agcttggaga atttacgata ttttgtgtaa
    27121 taacggattt acgaaacaat ctgcatgtgg aatattgggg aacattcaac aagaaacagg
    27181 aggaactttt gatcctgata ctgttcaaat aggtggacca gcatacggat tagttcagtg
    27241 ggatggttct tcatatcctc ttgttggtcc agcaacatgg gatggaaaag tttatgttca
    27301 aaacttattt aatgctgcag gtattaaaga accaataacg agcttagatg cacaagttcg
    27361 attgcttatt tggacattta caaatggaca atggatgggc gtagtacaac ctacgacggt
    27421 tgatggattt aaggcctgta ctgatccaag acaagcagca tatgcttttg aacgaaacta
    27481 tgaacgtccg gcagcgacac atcctgaacg tcaagattat gcagttaact ggtataacaa
    27541 atttaaagat ttaaaaccag gaggagctac tggagaagca ggactaaaac atttagagtc
    27601 tttgattgga caacgaattg gcaatggtca gtgttatggc ttgtctgcag aatattcagg
    27661 atatctgggt ggttgtggca tgggtgctgg aacaaaatat ggtttaactc atgtgatagg
    27721 aaatacttct gcagcatctg atattggtat tgcctatgat tggtctgctg ttggttggaa
    27781 agtgattcaa aaccctagat atgatcaatt agtagttggt gcaattatta attgggcaag
    27841 aggtggacaa gtgggttcat ggtttgcaga cggaacttat ggacatactg gcgttatccg
    27901 aggcttagct aatggtcgta tgcaaactta tgaacaaaac acagagttag gtatgatttg
    27961 tggaaagtta gatcgccagt attatagtgc aagtgcaatt tcttccattg tcataccacc
    28021 aaaataggga ggtgatttaa tggcaaagtg gaatgtcata ttaagtacaa cagaacccta
    28081 taattacgtg gggatgattc aagttcgaca aggcaataaa aacactgaag ttatggaagc
    28141 tacaattgtc gaaaatggtc ttccatatga tttatcagaa tgtaaggtat attttgaatc
    28201 agttgttggc ggaaaatatc cagtccaatt agaaacaaga attgtggatg ctaaaaaagg
    28261 gaaaatcaaa tatatttttg ataaatattc catgcagtgt ttgcatcgac aaacagccaa
    28321 tttcattata tttaaaggag aagacttgat tggaacaact caagacttct cctattttgt
    28381 catcaatgct gtttcaaaaa cagaaggaga aatgggttct tattggcaat caattgaaga
    28441 tttaattgct gatatgacag attttattaa tgaaaataaa ggcgatttta cggactggat
    28501 gaatgaaagg aaagaagaat ttgatcgctg gagagaagaa caggagaata gtttccaaga
    28561 ttggagagaa ggacaagaat cagattattt gtcatggttt gaatcaatca aggatatttt
    28621 gaagtctatc gatccaggtg gagtaatgtt agccgaatta atggatgcac gtgtagatct
    28681 tcaaggtgta cgccatgaat cgatttctga acgtttccta tccgatctaa attatttgta
    28741 tcaaaaaatg aaagcagcac ttttcacgat tgaatacggt gaaattgaag tgactgatat
    28801 tcttcaagac gatctctttt cagataatca cgaagtcgaa aaagttggaa ctgtagaatt
    28861 cccgattgaa gaaggagcct tgatcatcgc aaccgttgat gatccaaagc aaaatgtgtt
    28921 cactcttgag aaagtcgggg tgatctaatg gctaaaacta aacgaatgat ggaaacggat
    28981 gaaaaaacag gtgtacaacg ccaattcttt ccaatcacac atgcttccgc agttcttggg
    29041 ttagaagaaa taatagcagg agaagcaaca gttttatctg ttaatggaaa aattggagcc
    29101 gtcgtcatta ctaaagaaga tttgggatta gagaatgttc tcacagaatt accctatgca
    29161 agtgaagaag atgacggcat tatcacggca gaaatgtatc aaaaaatttt aaacagtgga
    29221 gaaggtgact acgtgttgcc agttgccact atcgaccgtt tgggtggcat aaaagttggt
    29281 gaattattga cgattgatga gacagggaaa gtctctgcag tcagacaatc tgatgtcaat
    29341 ttttcgctgg agttaaaaga aaaactcgat tcactgaaaa attatactgc tggagaaaac
    29401 atcactattg atgaagatgg aacaattagt tctacaggtg gtagtgggac tggtggtgtc
    29461 aatcagtcat atgtagatca aaaattccaa gaagctgtaa atcaagcaga aaattacaca
    29521 aacgaaagaa ttccaaattt tacttttgaa aaaattgggg aggtatagag aatgacagat
    29581 atcgtaaaaa taaaacaaag caatgtacag gtttatccac aaactcattg gaatgctata
    29641 gaaggcaaac caacaacggt gaagggagat aaaggcgatc ctggccaagc agcaacaatt
    29701 accataggaa ccgtttctag tggttcaacc gcttctgtta cgaatgtagg aacatcatct
    29761 gcagctagat ttaattttgt tttaccaaag ggagataaag gtgatcctgg aataaatgcc
    29821 acaactacag ctgtagctac aactacagca aatgggttga tgtcgtccac agataaaact
    29881 aagttggatg gaattgcagc tggagcacag aaaaatccag gtaatgctac aactacgaca
    29941 gcaggtttaa tgtcagcaac cgataaagtg aaacttgatg gattagccaa tattacattt
    30001 gagaaagtgg ggacggttta atggctgata ttgttcaatt aaaagaagac ggagttgcta
    30061 aatacttaaa aacgcatgta aaagctattg atggtaaaga agcattggtg cagacagatg
    30121 gagaccaagc cattgcagga cataagaatt tttcaggttc tgtaacaatc aataataaac
    30181 gtgttttgac gactgatgat aacagatatg aagtggtaaa tctggttgtc actaacggta
    30241 atacagggac agcaaagctt tatcgtgaag gaaaaacagt ttctatatat tttgtggctt
    30301 taaacggaaa aagtagtggc gggaatgatt cggttatttt aactgttcca gaaggctatc
    30361 ggacaccaat tagttttgaa caactggttg ggtcaataga ccgttctact ttgaacagtg
    30421 ctcaactatc tattggcgca gacggagcca ttaaatggag aagaaattca agttatggat
    30481 cagcttattc atttgttatc acttattcaa tttaagggag gaaatctaat gaaagtagtt
    30541 tacaaatcaa tcaagcctta cggattcgag caaatcattt tgaacaatca agaaaatatc
    30601 cctgaaaact gtacagagat taaaccacca gttcctaact ggagaccaag atttgatttt
    30661 gataaaaaac agtgggttga gttagctact gaagaagaga aaaaagggaa tgctgttgat
    30721 agcgtagatg aattggctaa tattaaggca ttatacgaaa cgctgaaggc agaaaatgat
    30781 gaattgaaac agctaaattc taaagcaatg ctaaacaatg tagcaatcaa gcaagaaaat
    30841 gtcttattga aagaaaagtc agaaagttta gcgcagttga attcaaaaac gatgcttgct
    30901 tctgtacaaa ataccaagga aatcgaagaa atcaaaaaac aattacaagg tgggaagtaa
    30961 catgtattca tatgaagata tcaaactaat gtatgactgg ggctgtttca ctaacgaaca
    31021 agtaatggtt ttcgttccat tatgcattac agacgaagaa gcagataaaa tcattaataa
    31081 agataagagc gcatcttaat tgatgcgttt ttatttaagg taaaggagtt gtcacatgat
    31141 taatttaggg gaatggggaa tgatagcagg atcaataacc gctatcgttt ctttgatttt
    31201 attagtaatt agaccgattg ctgcatcttt ctcgaaaatt actgagactc tttcaaaagt
    31261 aagccacaat ttagatttgc tgactaaaga tttagaatcg agcaaatcag atcgattgat
    31321 gattcatgaa gaactaaaga aacacgatga aagattagat acacatacag aaaaattggt
    31381 ggaacacacg caacaaatca aaactttgtt tagggagaga agaaaatgaa taataaaacg
    31441 ttcgaagtac taaaatggtt cgcactggta attattcccg cactagctac tttcgtgggg
    31501 ttagttggta aagcgctcaa ttggcagtac acagatatct gtgttgtcat cattactggt
    31561 tttggcgcgt ttttagggag tgtgttgggt gtatcaaatc gaacctacaa aatgttctcg
    31621 gctgaaagcg aagaaggagg aaaca
//
LOCUS       DACDEG010000020        31568 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.020, whole genome shotgun sequence.
ACCESSION   DACDEG010000020 DACDEG010000000
VERSION     DACDEG010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.020"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..222)
                     /locus_tag="IV142_001053"
     CDS             complement(<1..222)
                     /locus_tag="IV142_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484724.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKL"
     gene            516..1331
                     /gene="thiD"
                     /locus_tag="IV142_001054"
     CDS             516..1331
                     /gene="thiD"
                     /locus_tag="IV142_001054"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP8484725.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(1660..3111)
                     /gene="cls"
                     /locus_tag="IV142_001055"
     CDS             complement(1660..3111)
                     /gene="cls"
                     /locus_tag="IV142_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP8484726.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            3452..6118
                     /locus_tag="IV142_001056"
     CDS             3452..6118
                     /locus_tag="IV142_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8484727.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            6482..7234
                     /locus_tag="IV142_001057"
     CDS             6482..7234
                     /locus_tag="IV142_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8484728.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGGFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(7256..8410)
                     /locus_tag="IV142_001058"
     CDS             complement(7256..8410)
                     /locus_tag="IV142_001058"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP8484729.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            8595..11036
                     /locus_tag="IV142_001059"
     CDS             8595..11036
                     /locus_tag="IV142_001059"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP8484730.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(11366..12070)
                     /locus_tag="IV142_001060"
     CDS             complement(11366..12070)
                     /locus_tag="IV142_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484731.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYSLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(12257..12502)
                     /locus_tag="IV142_001061"
     CDS             complement(12257..12502)
                     /locus_tag="IV142_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484732.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(12519..13370)
                     /locus_tag="IV142_001062"
     CDS             complement(12519..13370)
                     /locus_tag="IV142_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP8484733.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            13536..14540
                     /locus_tag="IV142_001063"
     CDS             13536..14540
                     /locus_tag="IV142_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP8484734.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(14781..15137)
                     /locus_tag="IV142_001064"
     CDS             complement(14781..15137)
                     /locus_tag="IV142_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8484735.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            15505..16692
                     /gene="tuf"
                     /locus_tag="IV142_001065"
     CDS             15505..16692
                     /gene="tuf"
                     /locus_tag="IV142_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP8484736.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(16880..19471)
                     /gene="mprF"
                     /locus_tag="IV142_001066"
     CDS             complement(16880..19471)
                     /gene="mprF"
                     /locus_tag="IV142_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP8484737.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(19497..20216)
                     /locus_tag="IV142_001067"
     CDS             complement(19497..20216)
                     /locus_tag="IV142_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8484738.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            20536..21057
                     /locus_tag="IV142_001068"
     CDS             20536..21057
                     /locus_tag="IV142_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP8484739.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            21116..21976
                     /locus_tag="IV142_001069"
     CDS             21116..21976
                     /locus_tag="IV142_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP8484740.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            22083..24476
                     /locus_tag="IV142_001070"
     CDS             22083..24476
                     /locus_tag="IV142_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8484741.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            24658..26499
                     /locus_tag="IV142_001071"
     CDS             24658..26499
                     /locus_tag="IV142_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP8484742.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            26523..27632
                     /gene="rpoD"
                     /locus_tag="IV142_001072"
     CDS             26523..27632
                     /gene="rpoD"
                     /locus_tag="IV142_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP8484743.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            27852..29951
                     /locus_tag="IV142_001073"
     CDS             27852..29951
                     /locus_tag="IV142_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP8484744.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            30147..31010
                     /locus_tag="IV142_001074"
     CDS             30147..31010
                     /locus_tag="IV142_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8484745.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            31115..>31568
                     /locus_tag="IV142_001075"
     CDS             31115..>31568
                     /locus_tag="IV142_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8484746.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
BASE COUNT        10501 a         5540 c         6555 g         8972 t
ORIGIN      
        1 cagttttttc atctgtttat gaaattcttt tttttcagta aacataaagg tttgggtatc
       61 gtaaccttct tcatgtagga aatagattat tcctttttct gtgttttgcg tataagcttt
      121 tatactcctg tcttttttga aagaacagat cgtatagcca tctatttggt ctggtagtcg
      181 tttgattttc ttcaaaatga cttttttatc ctcaagtttc atttttttca cctgacttta
      241 tttttttcat atggaaatca tagcgccttt ggttatatga aaacaagaag gatactgaaa
      301 tatatataaa tcaaatagtg gttatttatt attagaatag cgaatacaaa taaacgacca
      361 ctattttctg atttattgca aatgtttttt caaataaatt tgattaccaa gaaaaggtag
      421 tggtatattt actgagggat actttgaaaa acatatgata gaaacagtaa cagtattatg
      481 aaaaatcttt ttataaaaat ggaggaatcg atttgatgga agatcagtat gtacaagcaa
      541 tgacgatcgc aggatcagat agcgatggaa gcgcagggat gcaagcagat atgcatacat
      601 tctttacaag aaatgtttac ggtgtttctg tcatgaccgc atgtgttgca ggtaattctt
      661 acggaatcgg agcaagtgtg acattgccaa cagattttat cgacaaagaa tttgaactga
      721 ttgcgaagga ctttcaaata cgtgcagcta aaacaggtat gctagcagat tcaaagttga
      781 ttgagacagt tgtaaaaaat taccaaaaat atgattttgg accattagtc gtggatcccg
      841 taatcgtaac gaaacatgga aatcttttac tagaagaatc tgctttgcaa agcttgaaag
      901 aaaagcttgt ccctttggcc gaagtactga cacctaattt ttatgaagca gaaaagctga
      961 ctggacggac attagcagct gaagaagatt ttgtgcaagc agcaaaagaa ctgcaagata
     1021 tgggtgcaaa aaacgttatg atcaaaggac atcatgctaa aaatgcgcag caagaaacag
     1081 tcaatgacta cgtattgttc gaatcaggaa aatcattttg gttaaatgca ccattttatc
     1141 ctaccgaccg gatcaacgga actggcgatt cgctatctgc atgtattgca gctgagttag
     1201 cgaaaggaaa aacagttgaa caagcgatcc gattggcaaa agactatgta aaccgtgcaa
     1261 ttcgtgaaga aataaatgta gggcacaaat atggccccat caatcattgg gcagctggtg
     1321 aattagagta aaagaaagaa gttgtgaaaa agttgccctg catcaagtaa taagaacagc
     1381 caagtaaaaa cagcttctta tgtttttaac ttggttggac ttattaccgc agatgcaagc
     1441 ttttgaacac cgtttaagaa agaggtcgtg agcctgccgt ccagctccga gtcacaagga
     1501 acaagttcaa aaaacagctt ctcatgtttg ttgaatattg tgacttgtga ccgaggagtt
     1561 ggcaggagaa caccgtttgt tcggaagaag ggactgtgac aagatttttg tcacagtccc
     1621 tttaatctta gtatattttt ctctacttga ataaaaaaat tataggattg gagaaaataa
     1681 tcgagaaatt tgttgcttga ataatagcca gttagacatt tccttgactg tttcagttgt
     1741 catggatgaa caatgtgtct catcttcttt gaactgagta gcaagttctt ctaaaaactc
     1801 aggatcgtaa atgacagcac ttgtttcaaa attcaatcgg tagctacgaa tatcttgatt
     1861 tgctgaacct accatgcaga tttcatcatc cattatcaat gtttttgcat ggaggaagcc
     1921 gttctcatag acaaggattt gtacattttc cttgatcagc tgccgagcat aatattgtgt
     1981 tgctcgataa ataaaaggat gatccggttt gttgggaatc atgattttca catctacacc
     2041 agaggcagtt gcgattttta aagcagcaat gacactttca tcaggaacaa ggtaaggtgt
     2101 ctgtatccaa acacgttttt tagcagaagt aatcaatttg ataaatgaaa gcttgatttg
     2161 ttccctttcg ttattcggtc cgcttgaaac aagctggatg gatgtatcag caagcttaga
     2221 ttcatctcga tctgcttttt taaagaaata attcaaatga tagccgacct ttttttcttc
     2281 aggtaccgag acgttccagt ctgttaagaa gcgaagctgg agcaaagaag aagcggcacc
     2341 gaaaatccgt atatgcgtat cgcgccaata gccgaatttt tttgttgtat ttacatattg
     2401 attggcaata ttgaaaccac cggtataact aatctttcca tcgatcacaa cgatttttcg
     2461 gtgatcatgg taattcaatc ggaaacgaag taatgccctt tgtgaggtaa caaatgtatg
     2521 gacatgtcca ccatttttga ttagacgatt gaaatctttt gcttttgtgc catgagagcc
     2581 aaatgcatca tataatattc gaacttccac gccttctgcg gctttttctt ctaaaacatg
     2641 taagacttgc tggccgatat tatctgtcac aaatgcgtaa tattcgatat ggatcgaatg
     2701 tttggctttt tttagatctt gaagcagtga atccaatttc tcttgtccgt ctgtgagaag
     2761 agtgacagaa ttcattcttg tcagcggcat acgatttaat gaagagaaga agtcaacaaa
     2821 ttgctgtttg tctttatcgc ccatgtcagg atcgtaatgt tcaatggtat ctcctctaaa
     2881 agactgaaag ttttctaatt ctttcaagtc actttgtcgg agatagaatt ttttcttatc
     2941 cgttaatcca cgtccgaaaa ataaatataa tacaaagccc accccaggaa gggcaaacaa
     3001 tactaagagc catgcccaaa tggctgctac atctcggggt ttgatcaaaa tagccaccaa
     3061 agcaataagt gcatttaata gataaagggc ggttataata ctagatacca caaaaatgtt
     3121 cctccttaca tacaataaca ttagtatagc atgatagaag aaagaagcct accaaggtga
     3181 atatactttt cactcctttc atgaaaatgt aggtaaatcg ggctaaagta gttgcttttt
     3241 gtaggttaat aaggtaggat attagtacag ttgaatatct ccttagggga gtaaccggca
     3301 acgtttgttg cgataatatc aacagtaagt taaccaaatg gttttctggt attgtatgaa
     3361 atggtgagac ctaagacaaa tgcgtatttg tcttaggtct cttttttttc agacaggact
     3421 agggccaaga tcaaaaaatg ggggatgaaa tttggaaaac aaggaacaag cacaacaagc
     3481 ggtctataaa gaaacgatcg actcgttgat gcagcaaacg cagagctcaa ccgatggatt
     3541 gagcaccacg caagcgaagg aaaaattaga aagagatggc ttgaaccagc taaaagaaaa
     3601 gccgaagaag ccaacttgga aattgttctt agaaactttc aaagatgcaa tggttatcgt
     3661 attgctgatt gtcgcactga ttcagatggt catgggtgac tacatcgaat tcttagttat
     3721 ttttgcagta ttgatgttga attctataat cagtgttgtc caaacaaaaa aagcagaagg
     3781 ttctttagaa gcgttgaaaa acctttcagc gcccgatgct cgagtgattc gtgatggtaa
     3841 ggaaaaaacg attccggcaa acgaacttgt ggtaggagat atcgtgttgt tagacgcagg
     3901 cgattatgta cctgctgatg gacgtttact agatgcggga tcattaaaag tagatgaagg
     3961 tatgctgaca ggagagtcga cttctgcaga aaaagaagta acggatatca caagtacggt
     4021 accaatcgga gatcgtacaa atatggtatt cagtggaacg atcgtaactt acggacgcgg
     4081 taaattctta gtgacttcaa ctgggaataa tacggagatc ggacaagttg ctggcctttt
     4141 ggaatcaaca actgagcaag tcactccgtt acaacgaggt ctggataaat tcagtaaaaa
     4201 actaagtttg gctattttgg cattgtcttt attgatttta ggtatccaac tgtttcgtat
     4261 ttatcttgga gaaggtacag gagatatgac tgcagatatc gtcagcgcag tgatgtttgc
     4321 cgtagccgta gccgtagctg ctattccaga agcattacag tctatcgtaa caatcgtgtt
     4381 atctttggga acgaataaaa tggcaaaacg tcatgccatt attcggaagc ttccagcagt
     4441 tgaaacactt ggttcgacaa gtatcatctg tacagataag actggtacat tgacacaaaa
     4501 caaaatgaca gttgttgact actttttagg taatggcgat actggggatt tcacaaatga
     4561 cccttcaaaa tggacagctg acgaacgtcg cttgatcgaa atcagtgttt tagcgaatga
     4621 tgcatcgatc agtgaagacg gtactaaatt gggcgaccct acggaagtag cgtttatcga
     4681 ctttagtgaa aaattaaacc agccttatca ggaaatccga aacaactacc caagacaagc
     4741 agaacttcct tttgactcag atcgaaaatt aatgtctact ggacacacaa ttgataacaa
     4801 aaattacttg tttgttaaag gcggaccaga tatcgtcttc caacgcagta aaaaagtatt
     4861 gctaaatggt gaagtcgttc cgatgacgga tgaattgatg aaaaaattcc aagagcaaaa
     4921 tgaagctttt tcaacacgcg cattacgtgt actagctttt gcttaccgtc cacttgataa
     4981 ttttgattta tcatttgaag atgaagacga tttgatcctt gttggtttgc ttgctatgat
     5041 cgatcctcca cgtgctgaag tgactcaggc ggtaacagaa gcaagaagtg caggaatcaa
     5101 aacaatcatg attactggtg accacaaaac aacagctgtt gctatcgcaa aacaaatcgg
     5161 gatttttgaa gaaggaaacc tggctttgac cgggacggaa ctagatgaat tgacagaaga
     5221 tcaactaaac gaagttttag aaaaagtcac tgtttatgca cgtgtttcac ctgagaataa
     5281 aattcgtatc gttcgtgctt ggcaaaataa aggtcatatc tcagctatga caggtgatgg
     5341 tgtaaatgac gcaccagcat tgaaacaagc agacatcgga attgctatgg gaacgggtac
     5401 agatgtggca aaagatgcag cagcgatggt attgacggat gataactttg cttctattgt
     5461 cagcgcagta gaagtaggac gtaatgtgtt tgataatatc aagaaagcta tttcttatct
     5521 atttgcagga aatttaggtg cgatcattgc gatcatcttt gccttagttg ctgactggtc
     5581 caatccattt acagcattac aactgttgtt catcaattta gtcaatgatt cactgccagc
     5641 aattgcatta ggtacagaaa aagctgaacc tgctacgatg aaccgcccgc caagagatcc
     5701 aaattcagga atctttacgg acaaaacatt gatttctgta acgtatcgtg gtgtgctgat
     5761 tgggattgct gttatctttg ctcaatggct tggcatgcag cattcacctg aattcggtgt
     5821 agccatgtca ttttcaacat tgatttggtg tcggacattg caaacattcc cagcacgttc
     5881 aaacacacaa acagctatcc aagcagggtt cttctctaac agaagtgtct tactagctgt
     5941 agttgtatgt agcgcgcttt actgcttaac aatgatccca ggactgaaag aagtcttctc
     6001 tatcccatca gcatttggat tgacagaagt ctttacatgt atgggaatcg cacttgtgac
     6061 aattgttttg atggaaatca ctaaattgat cattgtacga gtacaaggaa ataaataaaa
     6121 cgaatcaacg aggttgtgag cctgccgttt aattctgagt cacaaggtgc aatttcaaca
     6181 aacagcttct catgtttgtt gaatattgtg acttgtgacc gaagagttgg caggagaaca
     6241 ccgtttaagg aagaagttgt aaaatcagca ttttgagcta atactgaggg tcatctgatt
     6301 ggacactgtt tattcggaaa gagggacttt gacacgactt tggtcacagt cccttttttt
     6361 atttatgata gacggaaagc caatcatttt attattcatc aaaagagata gaatagataa
     6421 aagaggagaa tgatttattc cgaaaagtga aaacatataa caaaaaatta gaggaaatca
     6481 aatgaatgaa aaagaatggg atgaatttgc tgaagaatat tatgaaatcc agcaagaatc
     6541 gcaagtaact attgctgaag atgtaaaaaa ttatctgaaa acggagaaag tcctgcctgc
     6601 tgaaacagca gcagacgttg ctggaggatc tggcggattt ctttccttag caaaagaaat
     6661 taatcgctat gatttgatcg atttttcttc aggaatgcta gaaaatgcaa aaaaagaagc
     6721 agaaaaaaat gagttgccaa atgtttcctt tatcaaacag tcgttagaag aatttatcca
     6781 atcaaatgag acatatgaaa tggtttttac agcagcaaat ccagcattgg tacatcttat
     6841 tcaattagaa aaattgatga aaaagtcaag caaatggtgc gtgataatca gagtagtgga
     6901 atcaatcgat gatgtttttt atcctatcga aaagcaacta ggaatgatag aagaagatcc
     6961 taatacatcg ccagttgtaa tggatcaatt tgaaacatac ttaattgatg aaggattcaa
     7021 agtgaagaaa aaagatttta cttattttac acaggaatca attactgaag aattgatgaa
     7081 gctatactat gacgcgtacg caaaagatcc tgttgtgacc aggtacattc gcaaactatt
     7141 cagtgaaaaa cgagaaatcc ttagtacgac taagctgacc tatagactgc tcctttggtc
     7201 cgcagataaa aaaagaacct ttcacgactc gtgaagggtt ctttttatga ttttattata
     7261 ttttgtagta acgaatatca ttttcgatct ttgtaatcga aaaaggtaca tctgaagtat
     7321 attctgcaca ttctccgtgt tcaggaagcg tctcactgaa aatcgtttca tattcgtcaa
     7381 cagtgatccg tgttcgagaa tctagcatct cttgatgaga ttgtgcgaag agatattctt
     7441 gatagttttc ttccagataa cctgtaaaaa attcacttac tgcaccagaa ccgtagctga
     7501 atagaccgat gcgatctcct ggctgtagtg atttcgaatt ttccagtaga gaagttagcc
     7561 ccaggtataa tgaaccagtg taaagattac cgattcgtcg gctgtaacgg atgctttctt
     7621 catagcgagc cataagacgt tcctgatttt cttcgtctgt ttggtctaat acgctttgca
     7681 atgccttttt tcccatctta gtatacggaa tatggaaagc aatcgcctga taatcttcga
     7741 gtcctcgacc cgacaattct ttatgtcgat tccaaacttt ttggaatgat tcgatatacg
     7801 tagaattaga taaaggacca tcaacaacag gaaattcgct ataatctgga cgccagaaat
     7861 catagatatc ttctgtcaga aatacgctgt cgtcttcaat cgataaaata cgcgggtttt
     7921 gagtgatcat catcgcaaca gcaccgacac cttgcgtcac ttcaccaccg cttgccaagc
     7981 cgtaacgagc aatatcactt gctatgacta gtacttttcg ttctggatga tttttgacat
     8041 attctttcgc catgtgcaaa gctgcggtgc caccgtagca agcctctttt acttcaaaag
     8101 aacgagcaaa agattggatt ttcagtaaat ggtggataat taccgcactc gcttttgaat
     8161 gatcaatccc ggattctgtt ccgacgatga ccatgtcgat tagctcacgg tctttttctg
     8221 ttacgatttt gctggcagca tttgctccta gtgtaatgat gtcttcattt gcacgattga
     8281 ctgccatttg gtcttgacca atccctatat ggtattttgc aggatcatcc ccacggcttt
     8341 ctgccaactc tgtcatatct aaatataaat taggaataaa aaaggaaaga cgatcaatcc
     8401 ctattttcat ttcaacacct ctttacttat gataaaaaac ttactttact aaggtatcat
     8461 aaaaagatta aaagaaatat aaaaaagaga atatttaagg attgttaaaa tttaatctta
     8521 gaaaaaaata actggaacaa gagctatttg tttttacagc aaagaatggt atatttttgt
     8581 aggaggtggc ttacttgaaa gaagtcgtta tgatagatgc tgcaagaacg ccgataggaa
     8641 agtatcgcgg aagtttaagt ccatttactg cagttgaatt aggcacactt gtgactaaag
     8701 gattattaga taaaacaaaa ctgaaaaaag ataaaatcga tcaagtaata tttggaaatg
     8761 tcctgcaagc tggtaatggt caaaatgttg ccagacagat tgctttgaac agcggtctcc
     8821 ctgtagatgt cccagcaatg acgatcaatg aagtttgcgg atctggtatg aaagcagtga
     8881 tcttagcacg ccagctgatc caattaggtg aagcagaact tgtgatagcc ggcggaacag
     8941 agagtatgtc tcaagcacct atgctgaaac agtatcagtc agaaacaaat gaatatggtg
     9001 aaccaatttc cagtatggtc aacgacggat tgactgacgc attttcaaat gcacatatgg
     9061 gattaaccgc agagaatgtt gcaacacaat tttctgtgag cagagaagag caggatcgct
     9121 atgccttgtc gtcccagttg aaagcagcac atgctgtcga agccggtgta ttttctgagg
     9181 agatcatccc agtcaagatt tctgatgaag acgtgttatc tgaggatgaa gcagttcgtg
     9241 gaaatagtac attggaaaaa ctgggcacgt tacgtacagt attctcagaa gaaggaactg
     9301 taacagcagg aaatgcttcc ccgttgaatg acggtgcctc tgtggtgatc cttgcatcca
     9361 aagaatacgc agaaaataat aatctgcctt atttagcaac catcaaagaa gtagcggagg
     9421 tcggtattga cccaagtatc atgggaatcg ctccaatcaa agccattcaa aaattgacag
     9481 atagatcagg aatgaattta tcaacaatcg atctttttga gatcaatgaa gcatttgcag
     9541 cttcttctat cgtagttagc caagaactgc agttagatga agaaaaggtc aatatatatg
     9601 gaggagcaat cgcattaggc catccaattg gtgcaagcgg tgcacgtatc ttgactacat
     9661 tagcttacgg attgcttcgt gaacagaaga gatacggtat tgcttcatta tgtattggtg
     9721 gaggtttagg cttggcagtc ttgttagaag cgaatatgga gcaaacacat aaggatgtgc
     9781 aaaaaaaaaa gttttatcag cttacgccat ctgaacggcg cagtcaatta atagaaaaaa
     9841 atgtattgac acaagaaact gcgttgattt ttcaggaaca aacactttct gaagagctct
     9901 ccgatcacat gatcgaaaac caagtgagtg aagtggaaat cccaatgggg atcgcgcaga
     9961 attttcaaat caatggcaag aaaaaatgga ttccgatggc aacagaggaa ccatctgtca
    10021 ttgcagctgc aagtaatggt gcaaaaatct gtggaaatat ctgcgcagaa acacctcagc
    10081 ggctgatgcg agggcaaatc gttctatcag gaaaatcgga gtatcaagca gtgatcaatg
    10141 cagttaatca tcgaaaagaa gagctgattc tttgtgcaaa tgaaagttat ccttcaatcg
    10201 tgaaacgcgg gggcggtgtt caagatatct ctactcggga attcatgggg agtttccatg
    10261 catacttatc tattgatttt ttagtggatg taaaagatgc aatgggcgct aacatgatca
    10321 actcgattct tgagagtgtg gcaaacaagt tacgggagtg gttccctgaa gaagagattc
    10381 tattcagtat tttaagtaat tttgctacag aatcattggc ttctgcctgt tgtgaaatcc
    10441 cattcgaacg cttagggaga aacaaagaaa tcggtgaaca aattgccaaa aaaatccaac
    10501 aggcagggga atatgcgaag cttgaccctt accgtgcagc aacgcacaac aaagggatca
    10561 tgaatggtat agaagctgta gtggcagcta caggaaatga tacacgtgcg gttagcgctt
    10621 cgatccacgc ctacgcggca agaaatggat tataccaagg attaacagac tggcagatca
    10681 aaggggataa actagttgga aaactaacgg tgcctttggc tgttgccaca gtaggcggag
    10741 cttccaatat cctgccaaaa gcaaaagcat ctttagctat gttagatatt gattcagcca
    10801 aggaactagc acaagtaatt gctgcagtag ggttagctca aaacctggct gctttgcgtg
    10861 cattagttac agaaggaatc caaaaaggcc acatgggact tcaagctcgt tcattagcga
    10921 tctcaattgg ggctattggt gaggaaatcg agcaagttgc gaaaaaatta cgtgaagcag
    10981 aaaagatgaa ccagcagaca gctatacaaa tattggaaaa aatccgggaa aaataaaaaa
    11041 gcaatatctt tagaagaata aagcaagatt tacagttatt ttatttactg cagatcttgc
    11101 ttttttctct atcagaaaat atgtttatta ttagaatttt ttatagttaa ggttaggcat
    11161 cttaagaaat caggcaactc caagatatct tcaggtagaa gagaagtcgc ctaacattgt
    11221 aggatcatag gtgatttgtt ggacaggtca agctgatatt tgagcctgat gttagaagga
    11281 atttcttttt acttgctgta gaaattatta tatgactaaa aaggctggga ttttaaatcc
    11341 cagcctttta tgacaaatag aatttttatc gatcagtaat cgctgtcgct gcaaattgtt
    11401 cgatcaaagg aatcaaagtc aatttgactt tgtatttccg ttcgagtgct ttttcgataa
    11461 cgaactcatt ttcagtagtc atagaaatag ttgctaattt acgaggttca tcagcatttt
    11521 tttcattaat aactacttca tgttcatttt gttctgtgta aaatttagga tactcagcaa
    11581 cggcttttga aaaatcagca aaacttaaag cggcattcgt atcagttgag cgtgtatttt
    11641 cagttgtgtc cgcttttggt ttttccgttt tttcttctgg cacactgact gtttcttctg
    11701 taactggtgt cgcaacagct tgcgcagttg gttcatcgtt tttcaatcgt tgatcattcc
    11761 agtaagacac gatttcaata agaagattgt cacggaatcg ttcgaaaatc ccgttttctc
    11821 ctgaacgaat tctctttaat acagtattga agttgtcatc gattggcacg ttgatccgtg
    11881 tatcttcatt tgttttccca gctaatccga tcgttaagat ttgacggttt ttgggatcaa
    11941 gcatgacttg taacggcaaa ctataacgtc gcaagctctc ttcttctaga gaacggaata
    12001 attgtcctct tacaccatcc aatgaaggac ggaattgatt taataatttt tgtttctgtt
    12061 ttttcttcat atgttctctc cttcgctcat accctttcga ttataagaaa aaaacagcca
    12121 aatgaaaagg agtaatgcaa aaaaacttgc gtatacgcaa attacaacag aaaaatttgc
    12181 gtattttccc gttttttgaa agaaaaataa aaaatggaac ggattatgta tatatacata
    12241 atccgttcca ttctttctat tttttcggta tcaaggcaaa aacaggtagt tcaggtgaat
    12301 aaatgacttc atgttcgtgc cagggtttga ccattagcga atgatcgaac cgttttttca
    12361 gttcttcgat gatttgatca tgtgtccagt ttcttgcagg aaaatgttgg attttctctg
    12421 catcgatcaa aaagacaaaa tcggaatgaa aagtctgggt aacttcttcc gagagagaat
    12481 tcaagcgttc gattggatcc ataaaatcac ctttcgactt aaaattgggt agtatctggg
    12541 tcttttgctt tgccgactac accatctaac tgatcgattt gtttcatttc ttcttctgtt
    12601 aattcaaaat caaataattc agtattttct ttgattctgc ttggtgtcac tgacttagga
    12661 agaggaagga attcgtgttg cagcgaccaa cgcaaagcaa cttgtgcaat cgtcttgtca
    12721 tgtgcttcag cgatttgttt catttcaggc acatcgaaga ttttaccagt gcctaatgga
    12781 ctatatgctt cgaggatcat atcatgtttt tttgcatact caactacagt tgtctgtaat
    12841 tcgcctggtg caaggaaaac ttgattgacc atcggcataa tttttgctgt ttttgctagt
    12901 gtatctagat ggtggggtaa gaaattgctc acaccgattg ctttgatttt acctgcttca
    12961 tacagttctt caaatgctcg ccatgtatct gcatttgctt gttcccaatt atcacgaaaa
    13021 gcaacagggt ttggccagtg gatcaaaaac agatctaaat aatctgtttg gagcttcttc
    13081 aatgattctt caaaagaaga catcacaagt tcatagctgt ggtttgcatt ccataatttt
    13141 gttgtcaaga atatctcttc tctaggaatt ccactttcct taatcgcttg gccgacgctt
    13201 tcttcattct tgtatccttg ggcagtatcg atatggcgat aacctgcagc cagcgcttct
    13261 ttaacagagg aaacagcgac atccccatca ggtgtttgcc atgtgccgaa tcctactacc
    13321 gggatttcgt aaccatttga tagacgataa gtgcttgtta atgaattcat actttcagct
    13381 ccttttttat tcgttcaact ataagtgtag cgcatttttc gaaaaaaaga atccattttg
    13441 tttctactct tgaaattttt gtttaaaaga aaatgatttt gattctggta tttttgattt
    13501 tttttccgta aactaagaca gaagggaatg agaaaatgga tatctattta aaaaaagcaa
    13561 ttctgcatat tattgacaga gaaactggtt ctccagtctt ttcacaaaaa gagctggatc
    13621 tgacaaaaga atatattcgt gattttcttc aaaagaagat ccaaaaaatc tcttctgctc
    13681 aaacaaaaac tggacaacta gcagaagaaa gcatgatcgt tgaatcactt aaaatcgctc
    13741 aagaaaattt tgtggaagca agtgaagcaa tcgttcagcg gtggttcgat atctaccaag
    13801 aaagtgaaga agcacctagt gcagatgtat ttgttgtctt gtatgaatta gatacagtga
    13861 tgtacttatc tttattgaaa gtcaattatc gtgaggctta cactcacttt gttgaagcag
    13921 acgaagcagg gatcgacaac cgcttgattt tgaatcgagc aattctaggg gcgaaaacac
    13981 aaaaagcaga tgaagcattc gctgtgaatt tgactgattg gacctacgaa ttaattgaaa
    14041 aaaaatatga gttttctggc aaaaaagagc tttatttttc tggaagagtg atcgaaagtg
    14101 ttcctgcacc ttctttagaa gaaaatgtaa aagtgatcca aaaagtagcc aagcatcttg
    14161 gaaaaaaatt tgagacagaa gaatttgata tcatggctga tttgaaagaa gctgtctatg
    14221 atacgataga agaaaaaggt cggttggatc atgagatgat tgcggaaaaa gtcttcaagg
    14281 aaaacatcac ggcaaaaatg gcttttaaag aagaagtaca agagcaagga tttgtcccag
    14341 aagcacctcc tgtaaaagaa gtacaagaaa tttctgagaa aaaatttgga aaacaaaaat
    14401 tcaagctatc aaacgggatc gaactgatcg taccagtaga tgtatatcgc aacccagatt
    14461 tgattgaatt tgtgaataat ccggatggta cgatatccgt gatgatcaaa aatgtggatg
    14521 aagtattgaa tcgtttataa aaatgactag aatattaggt gttcaagtac cagtgatcag
    14581 ttaaatgaat tgtgtcaaga gaaggatcgg aaaagacgaa tataacaaaa aaagagcgtg
    14641 agacaaaagt aaagaacact tttgtctcac gctctaaaca cgtataaacg gcggaagcag
    14701 aagcaactcc ttcttatttc ttggagttaa acgcttctgt cccagcctct ttttatctaa
    14761 taaaatttta agaccattta ctaattatcg attttttctg ccgcattttt aggcacttca
    14821 gaagccttta tttcttccca gcttaataca cctttacgag gattgacttt gagtttaaga
    14881 tacgcattca tctttaatgg atgttctctt tcttcgcgaa gcgtcacctg ttttttttca
    14941 ccatctttat tataagccgc ttgcttgtag gtataagtcg tgaatttctc tccgttatct
    15001 gcttgggtag ctgacatttc gccattcgtc gtgatttttg tataataagc ttcgccgccg
    15061 taaaaataat gataagctgc aaaacttccg ccgagaaata tgcctaaaac gacaagtacg
    15121 ccaattaatt ttttcatgta cttcacctct ttccttatta ttaacgaaat agaagatttc
    15181 gtctattgaa agttgttggc aacagctgaa ttttaaactt ttcctcattt tgttcttatt
    15241 gcttcaaaag aaagttaagg actatcttga agtttttctc aaaaaacaca tttttttatt
    15301 tataaaaggt acttttttcg cgtataagac agacgagtgc tcttagttga ctttgaccaa
    15361 tattgacgta taataagctt agttcgaatc ttacaaaaag taagtcggac gtattctttt
    15421 tgatttagtc aaaaaaagac tataataaaa aggtgagagc tgaaattttt cagtgatcta
    15481 tattatttag gaggtttgta aattatggca aaagaacatt atgacagaag taaaccacac
    15541 gttaacattg gtacgattgg acacgttgac catgggaaaa ctactttaac tgctgcgatc
    15601 actacagtat taggcaaaaa aggcctagca aatcctcaag actatgctag tattgatgca
    15661 gctccagaag aacgtgaacg tgggattacg atcaataccg cacacgtaga atatgaaaca
    15721 gaaaaacgcc actacgcgca tattgatgca ccaggacacg cggactatgt gaaaaacatg
    15781 attacagggg cagcacaaat ggatggtgca atcttagttg tttctgcaac tgacggtccg
    15841 atgcctcaaa cacgtgaaca cattttattg tcacgccaag ttggtgtaaa atacctgatt
    15901 gttttcttga acaaagttga tttagtcgat gatgaagaat tgatcgattt ggtagaaatg
    15961 gaagttcgcg agttattgag cgaatatggt ttcccaggcg atgacactcc tgtgatcaaa
    16021 ggttccgcat taaaagcatt gcaaggcgat ccagatgctg aagctgctat tatggaattg
    16081 atggatacag tagatgaata tatcccaaca ccagaacgtg atacagataa accattacta
    16141 ttgccagtgg aagacgtctt ctcaattaca ggtcgaggaa ctgttgcctc aggtcgtatt
    16201 gatcgtggtg ctgttcgtgt cggtgatgag gtagagatcg tagggatcaa acctgaaaca
    16261 caaaaagcag ttgtaacagg tgtagaaatg ttccgtaaaa cgttagatta cggggaagct
    16321 ggggataacg taggcgtgtt gttacggggg atccaacgtg acgatatcga acgtggacaa
    16381 gtacttgcta aaccaggttc cattactcca catacaaaat tcaaagcaga agtgtacgtg
    16441 ttgacaaaag aagaaggtgg acgtcatact ccattcttca acaactatcg tccacagttc
    16501 tacttccgca caactgatgt tacaggaaca atcacattgc cagaagatac agaaatggtc
    16561 atgcctggtg acaacgtgac gattgatgtt gacttgatcc atccaatcgc tgttgaaaac
    16621 ggaacgacat tctcaattcg tgaaggcgga cggacagtag gttcaggtat cgttacagag
    16681 atcgaagcat aagaaacgcg actcgaatat tttggtaatg aaaaatgttt aaaataaaag
    16741 agcggtccaa acaggatttc tgtttggacc gctctttgtt cttcaattgt ataagaagag
    16801 attgtagaag catgaacttt cgttttactt cagtctcttt tattcatcat gagagtgttc
    16861 atctatcggt atgcagtttt taatactttc ttcgtatcca ttttttcaaa ccacttattc
    16921 taccttgtgt ctgctcaact ggcgcattat caataatcag caaagcaatc ataacatatg
    16981 caatccaact atctctagaa tatagagtat aacgtgagac ccagtttgta gcgtacttgc
    17041 ttttatattc tcttaatcca tggaaagaat aaaaatgaga accaaactcg taaaccaagt
    17101 atgcaatcct ttcctgtaaa aaactctttc tggaagttcc gacatttgaa agaggggcca
    17161 tccctaaatt gaaccattgg atatgttctt ctttcatata ggcaaacaaa tgcaagaaca
    17221 agaaatccat actgccagaa ggtgcttttt tagaatcata tcgcatcaga tcaatcgttc
    17281 ctactttatt gttcgtgtaa gtaggcatga tcgttgcaaa agcgacaatt tcttcttggg
    17341 tatttttgac aactgcaata ggcgctcttt gtagataatc ttcagagaaa aagcctaaag
    17401 aaaacccttt ttcctttcgt gaacccaacc aattatcaga gatatttttg aaagtactca
    17461 tttgctcagt agaaaatggt ggttgtaaga catcaaatgt aaatccttct cgttcgatcc
    17521 gattcaagac agcacgagtg cccttcattt ttttccctga agttgtaaaa ctattcaggt
    17581 ctacataagc ttcttccccc atcttaatga aatcgtagcc gaattcatgc aaaatcataa
    17641 caatctcttc gcttgtttcg tagaaaactg gtaaatagca tagtcgatct gtttcttcga
    17701 taaaagcttc gattgcttct ggaaaatctt ctttcttacc agaaggatca cccattacaa
    17761 tacatttatt attgaagcaa gcgaattgga gaagaacagt tggttcaccg tctttttcat
    17821 aggcaaacat ccgcttatct tttaagaaga tcaattgact atcggagttt cctccataag
    17881 ttgtaaggat cttcagggcc ttttcttcat taaaggcttc gccaatctgt ttcttttctc
    17941 cttgaaggaa atgaacaaat agaacgatca tgaaactaac agcgatgatg gccaacaatc
    18001 ccgaaaacca aatcttttca gaaggaaata agaaaaagga aataaaatgg tgacgccgat
    18061 gagggaaatc cggcagattg tataccccaa taacgatgta aagcaaggac agtgcaccaa
    18121 taatgattcc atcgatcgta cgccactccc atgaatagat caattgttcc cgaaataatt
    18181 cgtttttaga agcaagaatg atcagaagca gaatagacaa gaaaaccacc ggtgtgaaac
    18241 taaaatcact taataaaaca taaagtaaag ctagaacaat caagaagatt gtaggcaaat
    18301 atgctcgttt cacacgtgct gcgatccctc tgcccataat gattaaaaga aatcccaata
    18361 gtatactagg aaattggata ataaaatgaa atttcaatgg gttcaaacta tgaagccagc
    18421 gaaactctgt aaaagcttgc gggatagtgg cagaaagtac tagcatgatt ccagaaaagt
    18481 ataataagac aaccacgatt ttatgcgcaa tttctgttgc taattgttta gggataccag
    18541 aataacgctg atcaaaagtg gaaccgatat ttttgaagaa aaagacgatt ccaatcaaaa
    18601 acggaatgat gtagtagaac aggcgataga gcaagatcca cacaacgact atttctcttg
    18661 gcacaccaat agcggacaac ccgataatca tcattacatc gaaactccca atttctcctg
    18721 gaatcataga cacgattcct atcactgaag cagcaatgaa taatggaatt gtttgccaca
    18781 gatcaatccg gatttccatt aaataaccaa cagaaaggaa actaaacagg acaccggtcc
    18841 attccaacaa tgaaactagc aatagctgtc cttgagtttt aagtgaggta ccaccaagga
    18901 atcctttctt tttgaaagta gtaaataaaa agacgacagg aaaatataga ccgccgccga
    18961 tcaaccatat ccaatattgc ttcaaaaaag gagcaactgg cgtgaagact acaagtaaaa
    19021 aagaaagcag actgtaaata gaaagtccag ccatcaaaaa caagaaaact ttcgataatg
    19081 cttggatcac gtttctgctc tctttctttt gtccatatag ttcagagcga agccccacac
    19141 taacaaaccc gccgaaaccg gcaatattgt tgatcgtatt gatcagccag cttgtctcaa
    19201 acaggtaacg aggattctgc ttttgttcaa gcatccgatt caaaataata tcataaccaa
    19261 ccataggtaa cacagagata agaccaatca atagcattag tccgcttttc catacaggta
    19321 tagtggcaaa cgtttctcct aattgctgga tagatagagt tttaccgatg gacatcaatt
    19381 caccgacaat aatgacaatg acagaaatca aaaagattgt tttgaacagt cctaggtggt
    19441 ttttcagcca ttgtatggta ttttttaaca aataaatcac cttctattcc taatagttat
    19501 gtttcagaaa gaaattgaag aacttctcga ttgaagcgtt caggatcttt tctagctaat
    19561 tcatgtcctt gttctttcac aagcacaaag gaagcttttg gtatagtttt agctatatac
    19621 aaagaatgtt ttagcttgat tacatctttt tttcccacga tgatcaaagt gggggagttg
    19681 atttttttta aatcattttc tgtaagcccg atgtcatgca gcaaaagttt gataactaaa
    19741 agatttttgc gaagacttgg gcgaaataaa gagagtaacc atacccatgc ataatgaata
    19801 ttactgatga ctctggcaga aaaacgaacg cctttaacaa gcgtgtttcc agaatttaaa
    19861 atcaatcgat gaactttttt agggaagctg ctggcaaata ccagtgctag atttgctcca
    19921 tcactaaatc caagaaaatc tgcctgatcg attttttcca gcaacatgat cgtattcaga
    19981 tcctcggcca ttaactgaaa attcaacata gatgcttcgt ttgtcgaacg accatgtcct
    20041 cggctatcta ctagatacac ggtatagtag cgttcgagta cagggacctg ttctgaaaaa
    20101 aagcggccgc tgccgtcatt gccatgcagt aaaaagagag ggaagccttg accgctcttt
    20161 tcatagtaaa tttcgctccc atctggcatt tttgcatatt tcttttcttt tttcacataa
    20221 tcacctactg tgtaaagtat aacgattata tcagtcattg ggcaattgtt tttactgaaa
    20281 aacaacgatc attttcgtaa gatgtcaaaa aatacaggaa aattcatttt ttcttaaggc
    20341 gtacacagaa atcaaggaag aaatagccaa gaagataaag aaatagtaag ataaaaaaag
    20401 atgtcaaaat aactaggtga tgaaatgaag cgaaaacgat tatctattat ccgtattctt
    20461 ttttacatga tcattctgac ggttattggt gcggtgttgt ttttatgagg aagaacttat
    20521 cgttcggtaa aacgagtgga aagctatcaa atagaaataa caacagctgc aaaaaattat
    20581 catatggaaa actacgagaa tttgatcaag gcgataatat tgaccgaatc aaaaggaaaa
    20641 gggacagatc tcatgcagag ctcggaaagt ctgcatggag aagctgagat cattgataat
    20701 ccagctgaca gtattgatca aggtgtcaaa tatttggcgg aaatgatagt agaagcaaag
    20761 aaacaaggat gcgatttatg gacagctgtc caagcatata attttgggaa agattatatt
    20821 gcctatgtaa aaaatcatgg cggagaaaac acagtagaat tagcagaagc gtattctaaa
    20881 gatgtgctga gcccactttt aggaaatgag gaagggaaac aataccgata ttggcggatc
    20941 cagtccttgc tttacaatgg cggctatctc tatcataatg gaggtaacat gttttatcct
    21001 gatattgtca aaatgaatga aaaaataata gagacctatg aaaaagtctt taagtaaaat
    21061 agcaggaaat gttcaatcat gaataaaatt aaatgaaaga aggagtgcgc aaatagtgga
    21121 aaaaaggcaa aaaatggaaa atcaagaagt ggggtacaca accaaagact ggatgttacg
    21181 gtttgtgaag ggaatgttta tcggttcagg atttatcttg ccaggagtca gcggtggtgc
    21241 attagcagct gtctttggca tgtatgagcg gctgatttct tttttagccc accttacaaa
    21301 aaatttcaaa gaaaatgttt tgttttttct gcctgttggt ctaggcggag tgacaggcat
    21361 ctttctactt tcttttttag tcagtttttt attaggtgcg tatgaaacaa cgatattatg
    21421 gttttttgtc ggatgtatcg ctggaactgt tcctgcgtta tggaaagaag cagggaaaaa
    21481 aggaagagat acttctgatg tgatcatcat ggttgttact tttgttctgg cttatttgtt
    21541 tttattatac ggcgctcaat tatttgacgg gcaagtcgat cagaattttg gaacatggct
    21601 gattgccggc ggattgattg gtttgggtat gatcgtccca ggattgagcc cttctaattt
    21661 tttggtgtat atgggattgt ataaagcgat ggctgacgga tttaaagaag tacgacttga
    21721 agtgattgtg ccgatcggaa ttggtgggat tctttgtgtt cttggattgt caaaaatcat
    21781 ggattacatt ttctcaaagg catatagcaa gctattccat ttcattttag gtgtagtttt
    21841 tgcttcaacg attatgatca ttcctaccga ttacgcggga ttgggagttg ctggggtact
    21901 agcttgtcct gttcttttta tagccggtac agcactaggt tggtggatga gccgtttgga
    21961 ggaacaatat aagtaaagat agattgataa taaaaacagc acacgggtga ttcaaacaaa
    22021 aaggttggca tcttttttgt aaaatcattc gtgtgttttt ctataaattt tataaaaaca
    22081 agttgttttt aatcttggaa cacgctagac ttaaacacgt gattattttt gaaagttggc
    22141 ggaaaatgga gaaaaagaaa atgaatggac ttacacagca agaggtagtt cagaaaatgg
    22201 cttctggcca gcaaaatgac taccaggaag atgcatcaaa aagtacaaaa caaatattca
    22261 gcgataatat attgacgtta ttcaattttc tgaactttgg tattgggatt tgtctgttat
    22321 ttgtaggtgc gtattccaac atggcgtatt tagcaattat tgtcgtcaat atcgttattg
    22381 gaatttatca agaaatacat gcaagaaatc ttgtacggaa attatcgatt gtttcccaat
    22441 cagaagtact agtgatccgg gacggtaaag aacaatcgat ttctactaaa gagctggtat
    22501 taggagattt ggtaaaaatc ggagcaggag aacagatacc agccgattta caggttatat
    22561 ctggacgggc agaagcaaat gaagctcttt tgacaggcga atctgatttg attgtcaaag
    22621 aaactggagc tgaattgctt tctggaagct ttttgacaag cggttctgtc atggctgaag
    22681 ttatccgagt aggtgcagat aattatgctg tccgcttgac tagcgaggca aagactcaca
    22741 aagctattcg ttctgaactt gtagattcga ttcgcaaagt atccaaattc actagtttcg
    22801 tgattattcc tttggggatc atactatttt tggaagcact gttcatccgc gatgcttcta
    22861 tcaaaatttc tgttattgca tctgcagcag ctttgttagg catgcttcca aagggtttgg
    22921 tattgctaat aagtattgca ttaacgacag gggtaaccaa actggcaaaa aaacgaatcc
    22981 ttgttcaaga catgtattca attgaaacgc tggcccatgt cgatacatta tgcttggata
    23041 aaacagggac cattactgaa gggaaaatga gtatccagcg ggtagaagca ctacatccgc
    23101 tgtacgagaa agccatccca gaaattatgg ggacgtatct ggcagaaagc agtgataaca
    23161 acatcactat gcgggctatg agagattatt ttgagctttc taatcggtat gatgtgacaa
    23221 acaaagtcgc attttcttct gatcgcaaat ggggcgcgat ggaatttcca gaactgggag
    23281 tcgtgtacgt aggagcgcct gaacgtttgg tggaagaaga tcggcttccg gacagtctaa
    23341 aaacagccca agcgaatggc tatcgcgtac ttatgttggg gatcgctcca aatcagcaat
    23401 tgaacgaaga aaaaccagca tttgttgagc cggttgctct gtttgaaatc gatgatccaa
    23461 tccgaaaaaa cgctcaagaa acactagctt atcttcatga ggaaggtgtg gatctgaaag
    23521 tgatttctgg agacaatcct tttactgtct ccaatatcgc tagacgtgca ggattaccga
    23581 attatgatgc gtatgtcgat ctttctcaaa ttactgaaga aatggaagtg cgagaagctg
    23641 ctcatcgcta tacggttttt ggacgagtga gtccgcagca aaagaaattg ctggtaaatg
    23701 aattgaaaga aagcggcaga acggttgcaa tgacaggcga cggtgtgaat gatgttttgg
    23761 cgttaagaga agcagattgc agcattgcta tggcagaagg tgatggagca acacgtcaga
    23821 ttgcgaatct ggtattgttg gactctgatt ttactacatt gccagaagtc ctgtttgaag
    23881 gacgccgagt ggtcaacaat gtgacgaaag tctcaggcat cttttttatc aaaacgattt
    23941 attcatttat tttatcgatt atatgtgccg ttacagcaat tggatttccg tttatcccta
    24001 tccaaatcac gctgattgat ttagcgattg aagggtatcc ttccttcttt ttatcttttg
    24061 aacaagataa aagaaaaatc acttaccgtt atttgcctac agcacttatt aatgctttac
    24121 caaatgcctt gcttgtagta gtgaatatca ttgcggtgta tctacttgga aaaacattcg
    24181 catggggaaa tgctgaaact actactttga tgtattactt gctgattggg atcagttgca
    24241 tggcagttgt aaaagcttgt tatcccttca acccattgcg catcttccta gcagtaacga
    24301 cgatcgtagg gatatatgta gctgcaatgc tgttccatgg aattttggaa gtaaacttag
    24361 ggttgggaga gagcgtagga tacttctttg tattcatgct cgtaaatatt gttcttcgag
    24421 tattgtactg gaaggctgat attcaaaatc agttactgaa aagagtagca aattgatgct
    24481 taatgagata gaaatgtgtt atacttgaaa aagcaatgaa atatgataca atcaaacatg
    24541 tataattgtg tcctttaaaa tcgcactgat ttcagttagt gcgatttttt accggttatt
    24601 ctcctgaaat atcttctgtt aaacccaaaa gatatcaata gagcaggggg tgaaaaaatg
    24661 gctcaacgga ttcctcaaga ggttatcgaa acagtacgca gtcaaacgaa tatcgttgag
    24721 gtgattggac aatatgtcca gttgaaaaag tctggtaaaa attacttggg gctgtgtcct
    24781 tttcatgaag aacgtacacc gtctttttct gttgcagaag acaagcagat ttttcattgc
    24841 tttggttgtg gtaaaggcgg aaacgtgttt acttttcttc aagaactaga aggtctaagt
    24901 tttccagaag cagtggtaaa agtggcagac ttcgaacaga taccgttaga agatcaatgg
    24961 cgtcagcgta ctatagctgt ccaaaatcct gaatctgcta cggggaaact gattgctgct
    25021 catgaaaaag cagcggaaat ctatcatcac atgctattgc atacaaaagc tggacagcca
    25081 gctttagaat atttgcaaag tcgtgggttg tctttagaat taattgaaga attcaacata
    25141 ggttttgcac ctaatgagcg ttcttttctt cagcaagtat tcaagaatga atcaatttca
    25201 aatgaattgt tagaacgctc tggattgttt gtcacgcatg atgatggccg tatgtctgat
    25261 cgtttttatc aaagagtgat gtttccactt cgtaattctc aaggccagac gatcggtttt
    25321 tcaggacgct ggcttgcacc tgaaaaaggg caggaaaaag atcagcccaa atacttgaat
    25381 agtccagaaa cagaactgtt caataaaaga caagttttat ttaatcttga taaagcaaga
    25441 agcgatatcc ggaaaaacgg agaaattttg ttgttcgaag ggtttatgga tgtgattgct
    25501 gcatggcagg ctggtatcaa aactggatta gcttctatgg ggacaagtct aacagctcaa
    25561 cagatccaac aaatggaaaa gctgacgaaa gaattggtct tttgctatga cggagacaac
    25621 gcaggttttg aagcaaccaa tcgagggatt gaattgcttc ggcaaaacag tcgattagca
    25681 ctttctgtcg tagtcgttcc agaaaagctt gatcctgatg aatatctccg taagtatgga
    25741 gaagcatctt tccaagaatt agtggctcat gggcgcgaaa cagtctttac ttttaaatca
    25801 cgttatttaa aacaagggaa aaatttagat aatgaaaaag acaagatcga atacctcgaa
    25861 gaattactag ttgaacttag ccgagttgtt tcaccaatcg aacaagatat gtatctatct
    25921 caactgtcaa cagagtttca gatcggcaga gagacgatac aaaaacaact gcgcgatctt
    25981 cgaaggacaa atcagcaaaa tcgtcaagaa cctcaggaaa gtcaacacac tcaggttacg
    26041 agacaagaaa ctgcacggaa aaagagacct ttgacgcaag tagaaaaggc agagcagatt
    26101 ctattatatc gtgcatttaa agaagtttct gtccgtaata ttttaaaaga acgagaattt
    26161 cagtttattc atgatgcata tgcagaagtt tattttttat tcgatgcata tgtctcacaa
    26221 cattatgaat tcaaccttgc agagttttta gactttttgc aagatgaaaa tttaaggcgg
    26281 ttggtggtgg aaatcgaata tttaccggtg gcagaagaaa gttcgccacg agaaatccaa
    26341 gacttgctag atgtgatcag caaatctggc ttagctgatg aaataacatt aaaaaaacaa
    26401 atgcagcaag aagccagacg aactggaaac aaacagcttg agcttgaatt gacgattgaa
    26461 atcatcaatt taacgaaaca gttaaaacaa gccaaatagc tactcaaggg gggccttctt
    26521 ttatggcaaa tggaacagat aaaaaaacgt atgaagcagc agtagcagct ttcatcaaag
    26581 aaaacaaacc caaacgacaa gttgtatatg atgatttatc caatcagctt gctacaccat
    26641 tcactttgaa tgcagatgaa atggacaaat tgatccaaaa gattgaagac gcaggtatca
    26701 gtgtggtgga tgaaaatggt gatccatcaa ttcacagctt gaagagtgct gagaaaaaag
    26761 ctgaacaagc aaaaacagaa gatctttctg cgccaaccgg tgtaaaaatc aatgacccgg
    26821 ttcgtatgta tctgaaagaa attggccgtg tctccctttt aactgcggaa gaagaagtag
    26881 cgttagcatt gaaaatcgaa gaaggcgacc aagaagcgaa acagcgtctg gcagaagcaa
    26941 accttcgttt agtagtaagt atcgctaaac gttatgtagg acgaggcatg cagttccttg
    27001 acttgatcca agaaggaaac atgggactga tgaaggcagt tgaaaaattt gactaccgca
    27061 aaggatttaa gttttcaacc tatgctacat ggtggattcg tcaagccatc actcgagcaa
    27121 tcgctgacca agcacgtacg atccgtattc cagttcatat ggttgaaaca attaataaat
    27181 tgatacgtat ccaacgtcaa ttgctgcaag accttggaag agaacctacg ccagaagaaa
    27241 tcggtgcgga aatggatctt ccaacagaaa aagtccgaga aatcttgaaa atcgcgcaag
    27301 aacctgtttc ccttgaaacg ccaatcggtg aagaagatga ctctcactta ggtgatttca
    27361 tagaagacca ggaagcaaca agtccagcag aacacgcagc ttatgagtta ctaaaagaac
    27421 aattggaaga tgtactagat acattgactg atcgggaaga gaatgtcctt cgcttacgtt
    27481 ttggtttaga tgatggccgg acgagaacat tggaagaagt agggaaagta tttggtgtta
    27541 ctcgggaacg tattcgtcag atcgaggcta aagcattaag aaaattgcgc catccttctc
    27601 gttcaaaaca attaaaagat ttcttagaat aagcgtggac tattgaccag aagagaaatc
    27661 agtaaaactg tttggtttat tttaaaagaa aaaaagcttg agactacgca gtctcaagct
    27721 tttttttcta taggaaggaa attcattcat tatttttaag taaatcctaa aaataagcta
    27781 ttggcgtgac gtatagaata tgatagatta aatggtagcg taaacctagt aaaggagccg
    27841 atcgctaagt gatgaaaaaa tgtccaaatt gcggatcgaa aaaaatggag cagaatacta
    27901 tctgtccaga ctgcggtcat caaataactg cagaagatca gcaaataaaa gaatcaattg
    27961 aagaaacaaa tacagatgca gctatgcagg ataaatcaac tgaagatgca gtctttcctg
    28021 atacagaatt aaacgatcca atcgaatggt cggaattgaa agatttacct ctagaatctg
    28081 taatggaatt attcgaaaaa gcagaaccag aagacgaaga accacctgct cacgatcaag
    28141 taactaatat agagaaaaat gaacagaaga aagcaactgt tcatgagact tcggaagaat
    28201 caggaaagaa tcaagaagaa acgcttgaat caaaacaaaa aagaagagta gctgaactga
    28261 aagaaatcgt cgataatgag gaagaaaatt cgatactatc cgcttatatc aaagcacatc
    28321 gtgaagatac aaaagaagag cacgcgaaag agctgctcaa aatgatcagt gaaaaaattg
    28381 cacaggaaaa caaagtagag ccagaaaagg aacaagaaac tgaaaaagac tctgatagag
    28441 aaaacgccac aggtaaagaa acagtaaaaa taaaagacgt aaaaacggat atcacagaaa
    28501 aaactgaagt atttactgaa aaacaattag aaaaggaaaa aacgaataaa ctgccggaag
    28561 cagagaaaaa agaaatactc gaaaacacag acaaaaagtc aacagagccc gatgaaataa
    28621 atagtgaatc cgatgctgaa aatcaatctg taagcaaaga gggtactgat cttgaagcaa
    28681 aaatagaaaa agaagagaac cagcctaagc tgtttgaagc agaagcgaaa tcaataacaa
    28741 tcgtgccaga tactgaaaaa acacatcaag aatcaaactt agaaaaaaca gatccagata
    28801 aagcgcaaaa gaaaaaatca aaaaaagtac cttatctcgt tttagcagca gtacttctac
    28861 tcggtggagg aagctgggct tattatgacc atcatcaaaa agtagaagcc gaagtagcag
    28921 cagaagcaaa aagacagcaa caaatggaag aacttcatga gaaacttgct gccttttata
    28981 cagatgatca ccatcaattt attcgtgcag atatgattca tcaagattta tcaaaactaa
    29041 aaaatgattt atccagtctt aagaatgaaa aaggatattc agagttagaa acaacttacc
    29101 aagatatcca atctaagatc cgagatatcc aaaaagtgaa tgagtggttc atagcacctg
    29161 ccatcgtcga tgatcatcta gcagatgaca tcaagttgaa agcagataaa gagatccaag
    29221 caatcgaaac ggataacaca gattttggaa aattgattca acaagcacaa acagaagcta
    29281 aaaatcaata tgctgaattg caaacagcta aagaaaaaac agctatagtc tttaaagacg
    29341 gaaaagtagt ggattcagca acacgcgacc aatacaagac ggcaaaagaa gcagtagaaa
    29401 aagtgaaaaa tacagatcta tctgctcagt tgaatgaaca attgaaacaa gtagataaga
    29461 tgctcactga taaagagaaa aaagaagaag aggaaaagaa aaaagcagaa gaagcgaaaa
    29521 aagcagctga agaacaagca aaacaagccg ctcaagcagc agctgcagct caagccgctc
    29581 aacaagctgc ggtcgcacaa gcagcacagc aagaagcggc atcctcgacc ccttcctctt
    29641 caaactcagc gaatcagcca atcaaatctg ccagagcaag tgatattgcc gatgcatcta
    29701 atccagcatg gacgtgggca ccaggtgtcc aaagcaaagt gattgctaca tgtatccaac
    29761 gtggatatat cgttgaagga ggttacagat tagaaaaagc cagaatcgaa aatggggaag
    29821 gctattataa tctatatgcc acttcaacaa aatctgcatt gatgaaaggc attggagaaa
    29881 gtgctttgcc attttacatc gtaaccatca actgtaaaac tggatggttc ggaggaaatg
    29941 ggagcaatta attttaggca aatgaaagag gctgtgagcc tgccgtttaa caccgtttat
    30001 tcgaaaaagg ggagtatgac acaactgttg tcatactcct tttttcacct ttccaaacca
    30061 aggctcagct attcaagtag ataacattat gttatgatag caaagaaatg tattaagaaa
    30121 acctttcact atagagggag aaaaaaatga acgatttatt agcaactata tttacaggta
    30181 ttattattga tgaaaatgaa aaaaactact ttgtccaaaa aaacggacaa acctttggct
    30241 taagcaaatc agaaggtgaa cacaaaatcg gtgaagcagt tgaaggattc ggttaccaaa
    30301 atcaaaaaca tgaaaatcgg atcactacac aaattcctaa aagccgtatc ggtcattacg
    30361 cttttggaat cgttactgct acacgaaaag atttaggtgc gtttgtcgat atcggtcttc
    30421 cagataaaga ctttgtcgta tctcttgatg aattgccgac aatgacagag ctatggccta
    30481 aaaaagggga tcgcttgatg atttccctta gagttgatgc aaaagaccgt atttggggca
    30541 gtttagcaga tgaaaaaata ttcaaatctt taagcaaata tggaacagaa gaaatgaaaa
    30601 acaaagatgt gataggaaca gcataccgct tgaaattaac aggtacgtac gttttaacgg
    30661 aagatttcta tcttgggttt atccatcctt ctgaacgata taatgaacct cgtctaggcg
    30721 agcagataaa aggtcgagtg attggtgtcc gaccagacgg tgtactgaat ctttcattga
    30781 agccaagagc atatgaagct atatcagatg atgcacaaat gattttaacc attttagaac
    30841 gttctgcaga tcagcaaatc aactttacag ataaatcgga tccagaagaa atcatgaaag
    30901 cttttggtat cagcaaagga gcatttaagc gcgcattagg taatcttttg aaacaaggat
    30961 tgattacaca agaaaacggt gtgacaaaac tagtcaaaaa atctaattga gaatcccttg
    31021 catgtttttc ttaactagct tataataatt ttaattaaca tagtgttata tcttttttta
    31081 tttgtaatta ttataaattg ttggggttga gtaaatggtc atgaatgcta tttcggcgat
    31141 gaaaaaaaca aaacagcagc tacatgaagc ggggtttaag ctgacaccgc aaagagaagc
    31201 tactctgctt gtgcttttgg aaaatgagaa agagcattta tctgcagaag aaatcttctt
    31261 tttagtgaaa aaaaagagtc cggaaattgg acttgctact gtttatcgaa cccttgaaat
    31321 cttgacagaa ttgaaaattg tcgataaagt aagttttaat gatggattag cccgttatga
    31381 tttgagaaaa gaaggcgcca aacatttcca tcatcattta ctatgcttga actgcggcac
    31441 gatcgaggaa gtagaagagg atcttttact tgaagtggag caagtagtag aagaacgtta
    31501 tcatttttta gtaaaagatc atcggctaac gttccatgga atctgtcagg aatgctatga
    31561 aaaagaaa
//
LOCUS       DACDEG010000021        31181 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.021, whole genome shotgun sequence.
ACCESSION   DACDEG010000021 DACDEG010000000
VERSION     DACDEG010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.021"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            110..1246
                     /locus_tag="IV142_001076"
     CDS             110..1246
                     /locus_tag="IV142_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8484747.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            1383..1727
                     /locus_tag="IV142_001077"
     CDS             1383..1727
                     /locus_tag="IV142_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP8484748.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            1998..3284
                     /gene="efmA"
                     /locus_tag="IV142_001078"
     CDS             1998..3284
                     /gene="efmA"
                     /locus_tag="IV142_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP8484749.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(3422..4021)
                     /locus_tag="IV142_001079"
     CDS             complement(3422..4021)
                     /locus_tag="IV142_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP8484750.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            4425..5546
                     /gene="mnmA"
                     /locus_tag="IV142_001080"
     CDS             4425..5546
                     /gene="mnmA"
                     /locus_tag="IV142_001080"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP8484751.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            5792..7213
                     /gene="arcD"
                     /locus_tag="IV142_001081"
     CDS             5792..7213
                     /gene="arcD"
                     /locus_tag="IV142_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP8484752.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            7227..8345
                     /locus_tag="IV142_001082"
     CDS             7227..8345
                     /locus_tag="IV142_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP8484753.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(8405..9697)
                     /locus_tag="IV142_001083"
     CDS             complement(8405..9697)
                     /locus_tag="IV142_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8484754.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            9847..10407
                     /locus_tag="IV142_001084"
     CDS             9847..10407
                     /locus_tag="IV142_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8484755.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            10536..10913
                     /locus_tag="IV142_001085"
     CDS             10536..10913
                     /locus_tag="IV142_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP8484756.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            10846..12918
                     /locus_tag="IV142_001086"
     CDS             10846..12918
                     /locus_tag="IV142_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8484757.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(13129..14076)
                     /locus_tag="IV142_001087"
     CDS             complement(13129..14076)
                     /locus_tag="IV142_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8484758.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            14322..14786
                     /locus_tag="IV142_001088"
     CDS             14322..14786
                     /locus_tag="IV142_001088"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP8484759.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(14848..15891)
                     /locus_tag="IV142_001089"
     CDS             complement(14848..15891)
                     /locus_tag="IV142_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP8484760.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(16268..17233)
                     /locus_tag="IV142_001090"
     CDS             complement(16268..17233)
                     /locus_tag="IV142_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP8484761.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            17467..18312
                     /locus_tag="IV142_001091"
     CDS             17467..18312
                     /locus_tag="IV142_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8484762.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            18503..19690
                     /locus_tag="IV142_001092"
     CDS             18503..19690
                     /locus_tag="IV142_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8484763.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            19696..20052
                     /locus_tag="IV142_001093"
     CDS             19696..20052
                     /locus_tag="IV142_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP8484764.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            20054..20392
                     /locus_tag="IV142_001094"
     CDS             20054..20392
                     /locus_tag="IV142_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP8484765.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            20787..21821
                     /locus_tag="IV142_001095"
     CDS             20787..21821
                     /locus_tag="IV142_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8484766.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            21814..22500
                     /locus_tag="IV142_001096"
     CDS             21814..22500
                     /locus_tag="IV142_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8484767.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            22524..23372
                     /locus_tag="IV142_001097"
     CDS             22524..23372
                     /locus_tag="IV142_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8484768.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            23569..24342
                     /gene="sufC"
                     /locus_tag="IV142_001098"
     CDS             23569..24342
                     /gene="sufC"
                     /locus_tag="IV142_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP8484769.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            24355..25641
                     /gene="sufD"
                     /locus_tag="IV142_001099"
     CDS             24355..25641
                     /gene="sufD"
                     /locus_tag="IV142_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP8484770.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            25638..26873
                     /locus_tag="IV142_001100"
     CDS             25638..26873
                     /locus_tag="IV142_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8484771.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            26860..27330
                     /locus_tag="IV142_001101"
     CDS             26860..27330
                     /locus_tag="IV142_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP8484772.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            27335..28726
                     /gene="sufB"
                     /locus_tag="IV142_001102"
     CDS             27335..28726
                     /gene="sufB"
                     /locus_tag="IV142_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP8484773.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(28821..29963)
                     /locus_tag="IV142_001103"
     CDS             complement(28821..29963)
                     /locus_tag="IV142_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8484774.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(30002..30736)
                     /locus_tag="IV142_001104"
     CDS             complement(30002..30736)
                     /locus_tag="IV142_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8484775.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            30968..>31181
                     /locus_tag="IV142_001105"
     CDS             30968..>31181
                     /locus_tag="IV142_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8484776.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKI"
BASE COUNT        10141 a         5713 c         6575 g         8752 t
ORIGIN      
        1 tttttctatg cccatgaaac gtcctctaga caaaaatgag ctcttctttg gtttgttcgc
       61 tattttatgc tagaatgtgc ctagcaataa tttggaaggg atgaatgaag tgaaaactat
      121 ttatctggat catgcggcaa ctacccccat gcatccacaa gtaattgaag cgatgacggc
      181 aatcatgcaa gacacgtttg gaaatccttc aagcatacat ggatttggtc gccacgcgca
      241 tgaaaaattg gaaacagcaa gacaaatcat tgcagacagt ctgcaagcaa aacctcacga
      301 gatcatcttt aacagtgggg gaacagaagg ggataataca gctatcttag aaacagcgtt
      361 ctcgcgtcaa aaagaaggca gacatctaat cactacagct atcgaacatc ctgctgttct
      421 taataccatg aaatatttag agacaaaggg gtttgacgtt acatatctgc ctgtagatga
      481 aaaagggaat ctgtcgatcg aagaggtcaa aaaagcgtta cgagaagata cgatcttggt
      541 ctctgtgatg tacgggaaca atgaaatcgg aaatttgatg ccaatcaaag aaatcggtga
      601 acttttagcg gatcatccag cttatttcca tacagatgcg gtacaagcgt acggaaacca
      661 aacgatccat ccagaagaat taggaatcga tttattaagt atctctgctc ataaaatcaa
      721 tgggccaaaa ggcgtcggtt tcttatataa acgagacgga gtaaatatcc ctgcattgct
      781 tttaggcggg gagcaggaag aaaagcggcg tgcaggtaca gaaaatttag cagggatctg
      841 gggtatggca aaagcagttg aactgctgtc acctgaagaa cgtgaagcac gtaacaacaa
      901 atataaagga ttccagcaat tgattcttga tacattagaa gcaaacgaaa tcccttatca
      961 agtaaatggt gatttaacga ataaactccc tcatgtgttg aatcttcgct taattggcga
     1021 agcaaatgat ttgctactga tgaagcttga tcttaatggg gtcgctattt ctactggttc
     1081 agcttgtaca gcaggaaata tcgagccttc acacgtatta gaagcaatgt atggagaaga
     1141 tacaccgata ctaaaagaat ccatccgtat cagctttggt ttgggaaata ccgaagacga
     1201 catccagtat ttccttgatc aattagttcg tgctgtcaaa aaataactag tgaagtcatt
     1261 cttatatgaa cagatgtctg agagaacgcc ttcatttttt agggaactta actgaaaaac
     1321 ctagtaaaaa tcaggataaa cttttataat aaagagaaac taattttatc gaggtgaaaa
     1381 tcatggcatt tgaaacaaca gcaacggtac tgggcgcacc aattagctat cgtttgcatc
     1441 ctgatgcaaa aagatataca ttgcgcgata atggatttac agaaacaaac ggaggcaatt
     1501 atcaattgat ccgaccatta gatgccacgc cgcaaagcaa agaaggattc aaattaaaaa
     1561 tcactgtatc aaaagatatt caaacattga aaatgtcgat cactacagca aatggattga
     1621 aagcagtcaa tattttcaaa gatccaaaac agaaaatgag ccaagataaa ttttattttc
     1681 tgatggatgg gatgattagc cgtgggttat tcgaaaaagt cgaatgatct gtggatataa
     1741 aaagacaact agttttgaaa cggcctattg ttagatgact aactttgtaa ggacgacgca
     1801 aattagttgt cttttttatt tttaaattta acacatgttg ttatttttaa tattttgctg
     1861 atttattgtt tctaagtaaa gaaatttaaa ttgaaacgat tgttatttag ggaaaaaggt
     1921 attttttgaa aaaagaatcc cgtataatga ttgttgtgca aacgctatac aaaatgaaaa
     1981 agaaaagagg gatgtttatg gaaaatgaac agtcggtcgt tttaacgaat tggaagagaa
     2041 attatttgtt ctttttatcg ggacagtttt tgtcaggaat cactagtatg gtcgtccaat
     2101 atgcgatcat ctggtatttg acaagagaaa ccggttcggc gacaattttg agttttgcta
     2161 ctttactagg aatgattccc atggttttac taagcccatt tgtaggaccg ctagtggatc
     2221 gctgggataa aaaagctctt ttgattgtca cggatattat tgtagcgatt ttcgcgctta
     2281 ttttagcagt agtcggaacg atttcagaat ccttcccgat ttggctagtt tttgtgtctt
     2341 tgtttatgcg ttcggtcgca caaacattcc aaatgccaac gatccagtcg attatgccga
     2401 caatcgttcc atcttctcac ataacaagga cgaatggaca gttaggaatg gtccaatcag
     2461 cgaatttcat cattgcgcca gctctaggtg cggctttgtt ttcagtcgtt cctgtcaatt
     2521 accttatttt attagatgta ttgggggcag tttttggtgt cggcttattg atttttgtga
     2581 agatcccaaa agtttctcct gaaatattgg aagttccgct tactattttt aaagatgcaa
     2641 aatttgggct gcagcaattg atggataata aagggttatg gtacataacg atcaatggtg
     2701 catttgtgat gctgttgttc atgccagcaa tcagtctgta tccattgatg acgctagatt
     2761 actttggtgg ttccgttgga caggcgggag cagtggaagt agtctacgca gtcggaatgc
     2821 tcttaggcgg tgcactgatc agttttatag gaacatggaa agaccgaatg aaacctatta
     2881 ttatagccta catcattatg ggcctgacga tcggtgcaag cggattggtt ccaaacgata
     2941 gtcaagggtt cttgtacttt cttatcttga atgcaggagc aggttgtgca acgccatatt
     3001 tcaatacact gctgatggca atgatccagc agagttatga atcgaatgtt ttaggtcgtg
     3061 tcttagggaa cttcaactct ttgatgaacc tggcaggtcc aattggatta ttatttgcag
     3121 gaccattagc tgatcgttta ggggtagaaa aaatgttctt gttttccgga atcgggattt
     3181 tattatgcgg aatcgtttta tttttaacgt cagctgcccg aaattatgat aaagaattac
     3241 agaaaaaact ggtaaaagaa caccatgagc aaaaagacga atagtatgga atggagttgt
     3301 gacagaagtc ttgttacaac tccatttctg atcctaatgt tcctgtggca ataagcctac
     3361 tcaaatgaaa aatatgagta attatcttta tttgtttaat acttgatata gggcagtttt
     3421 ttcacgacct atcgtacttc ctctttgctt catagatttc tttgatttgt tgctcagata
     3481 acggagtgcc aaatggtagt ttttccttta agagttctct ttccggtgcg tctacgccta
     3541 cattgacatc gttctcgata ggtacgctgt aatgatggat atgtccatga agattgcgga
     3601 tagtgtcgtt aggcccgagc aacatcggat aatgagtcaa gtaatattgt tgatggtcga
     3661 atttaacgat ggcacctaca tcataaaaat aaaatttggg acttccgtca gtaagcctag
     3721 gatcattttt ttctaaataa cggaaaaaag cgcgactgtc atggttccct ttgatcaagt
     3781 ggattgttcc ttgcagttga gtgaccagtt ccaaaatttc tgcatttgct ttttcatatt
     3841 gcggatgcat agcaaggtcg cctagatgat aaacatggtc ggtttctttt attactgcat
     3901 tccagctagt gatcaactgt tggttcattt catccacatt tgcaaagagc cgcggagcaa
     3961 agtcgttttt tcctaataag cgttcatgga aaaagtgcat atcactgata aagtaattca
     4021 tgtatctttt gcctcccttt tttcttagag taaacaaaat cacttatctg agcaactcat
     4081 aacatctaaa aagaaaaggt tgtaaatgag acgtgcagct ttaagcgata agaagcaatt
     4141 ttgaaaaata gtttttcaca atttataaaa ttattcctta ttgccgaaag gctggccctt
     4201 tttatgagta tgccaatttt ttatatcgaa tgctcaaaag atgagtgatt ttttggttga
     4261 ctatcatgtt tgtgtactga ataggtccag tagaacagta tttttacgac ttgataatta
     4321 agggatgaaa ttttcagaaa tatgacgtat aatgtaaaat actgaaaagt tgcgaccttc
     4381 aactcgtaga tgtttcagaa tctaattgaa atgatggtga tatcatgaca gacaacagca
     4441 aaacacgcgt tgtcgtaggg atgagcggcg gtgtcgactc atctgtaaca gcacttttgt
     4501 taaaggaaca aggatacgac gtgatcggaa tattcatgaa aaattgggat gacacggacg
     4561 aaaacggtgt atgcacggcc acggaagatt ataaggatgt agcgaaagta gctgctcaaa
     4621 tcgggattcc ttattactct gtgaattttg aaaaagaata ttgggatcgt gtatttgaat
     4681 atttcctagc cgaataccgc gcaggacgta cacctaaccc agacgtgatg tgcaacaaag
     4741 aaatcaaatt caaagcattc ttggattatg caatggatct tggcgccgat tatgtagcta
     4801 ctggacatta tgcgcaagtc actcgcgatg aaaacggcgt ggtccacatg cttcgtggag
     4861 tagacaataa taaagatcaa acttacttcc ttagccaatt atcacaagaa cagctttcaa
     4921 aaacgatgtt ccctctaggc ggcatggaaa aatcagaagt acgggcaatt gctgaacgcg
     4981 caggccttgc gactgctaag aaaaaagatt caacgggaat ctgctttatt ggtgaaaaga
     5041 acttcaaaca atttttgagc aactatctgc cagcaaaaaa aggaaatatg gtcactcttg
     5101 atggtgaagt caaaggacaa catgctggct tgatgtatta tacgattggt caacgtcaag
     5161 gactagggat cggtggcgga ggagattcgc aagaaccctg gtttgttgta ggaaaagatc
     5221 tagcgacaaa cacattatat gtggggcaag ggttccatca tcctgcattg tatgccacaa
     5281 gcttggatgc aagtgaaatc cactttacga cgaatgaacc aatgccaaaa gaattcaagt
     5341 gtacagcgaa attccgttat cgtcagcaag acgttcctgt gacagttcgc ttattggatg
     5401 ataatcgtgc agaagtcgta ttcgacgaac ctgtacgtgc aatcactcct ggacaagcag
     5461 ttgtatttta tgatggaatg gaatgtctgg gtggtggttt gatcgaccac gcatatcaag
     5521 aaacaaaagt tttacaatac gtttaagcga tattaaatga atcaaaacag ctatggctaa
     5581 caaaagaaac accttttctt ttggatagca tagctgtttt tttgcaataa aattaatttt
     5641 tattcatttt atacttgaaa tacttgtgta aaaagttcat aatatccaat aatatgaata
     5701 aagttgcttt ttgttaagca agagcagttg caaaatagaa gggaatcttt tgcaagtgat
     5761 ttgttcaatt ttataagtga gggtgatgga aatggaaaag aaatcgacgg gtattagcaa
     5821 atggggactg gttgctctgg ttgtcagttc gtcaatcgga agcggtgtgt ttgggatcac
     5881 aagtgatctg gctagttctg cagctccagg gccagcgatc ctttcatgga taatcgttgg
     5941 gattggtatt ttagctttgg tattatcgct gaatcattta ggagaaaagc gtccagatct
     6001 agatggtggt attttcggtt acgcggaagc ttcatttggt aaattaggtg gcttcatcag
     6061 tggttggggt tactggttgt ctgcgtggct gggaaatgtt gcttttgcaa cgatgctgat
     6121 gagcgccatg ggagaatttt tcccgatatt caaaggtgga caaaatgttc cttctatttt
     6181 atttgcaagt atttttattt ggggtctgac tttattagta aacaatggag ttgaaggagc
     6241 gagcgtcatc aatacaatcg tgacgatctg taaattagtg ccattattcc tatttttaat
     6301 atttgtattt atcgcattta aagtgaatat attcactgct gatttttggg gtaacgtatc
     6361 tgataatcta gtgaatggaa cagggcagca aggaacgatc tggttgcaga ttaaaggttg
     6421 tttgatggtt atgatgtggg tctttgtagg gatcgaaggc gcaagtgttt tagccaaccg
     6481 cgcgaagaaa cgttcagaag cccagcaagc atcgatcatt ggtctgcttt gtttattgtt
     6541 tatctatatc cttgcttcga tcttgccata cggcgtctta tctcaagaag agttagcaac
     6601 tatttcacaa cctgctatgg caaatatttt aaaaggaatc gtgggaaatt ggggagcagc
     6661 gctgatcaat atcggcttga ttatttcgat cattggttct tggttgtctt ggacgatgct
     6721 cccagcagaa acaacgatgt tgatggctcg agacggtctg ttgccgaaag gttggggaaa
     6781 aacgaataaa aaaaatgccc caacttattc gttagttgtt actgcaatct tgactaatct
     6841 attcttattg actttcttag ttactgatta tgcctatcaa tttgcctatt ctttatgtac
     6901 agcggcaatt ttgatttgtt atctacttgt cgggatctat caagtccagt tttctttaaa
     6961 agaaggaatc aaaaaacaag tagtgatcgg tgggattgct gtagtttttg aattgatggg
     7021 catcatttta gcaggatttt cttatgtcct tttatgtagt attgcctatc ttcctggttt
     7081 ttgcttctac tggatcgctt gtcgtgaaac caaacatcaa attacgggaa aagaaaaagt
     7141 aatgattggg ctgatttgtg tggccgcact gctttcaatc gggttgcttg ctgtaggctg
     7201 gatcaagata taagggggaa agaaacatga aaatcgcaaa tttcggtgta gaagaatggc
     7261 taaataaatg ggaaaagaaa gcggtatatg atattgcaca aagctcgatc gaagcattga
     7321 cattagacga actcgttggc ttagatggga ctagcgtaag taaatttttc gaaaaacgca
     7381 aaaacgaacc attggactat ggctggatcg aaggatcaga tgttttcaaa caagaagtcg
     7441 cttctcttta tcaaaccgtc gatccggaaa acatcttaca aacgaatgga gcgactggtg
     7501 ctaatctttt agctctatac gcgttagtgg aagcaggaga ccatgttgtt tcgatgcttc
     7561 ctacttatca gcaattatat gacatcccca aatctttagg tgccgctgtt gactttgttc
     7621 atttgaagga agaagaagat tggcagtttg atttagaaca gctagaagcc ttagtcaaac
     7681 ctgaaacaaa aatcatctgt ttgaatagtg caaacaatcc tacaggcaca ttgctagatc
     7741 ggaaaacttt agaaaaaatc gcagaaattg ctcgtacagt agatgcatat gtattgatag
     7801 atgaagtata tgcgccttta acggataaag gtgaattctt gtcgatcgta gatgtctatg
     7861 aaaaaggtat agcaaccaat tccctatcca aaacctactc cattccaggt attcgtattg
     7921 gctggacagc tactggacct gagttggcag aagtttttcg aaagtatcga gactacacga
     7981 tgatttgcgg cggagtcctg tcagatgatc ttgctgtaca tgcattgaag aataaagaaa
     8041 aaatattggc gcgaaacaaa aagatcatta cagagaacct tgtgatttta aaacaatggg
     8101 tagccaatga accgaaagtt gaacttgtcg ctcccaatta cgtctctact tctttcatta
     8161 aattagccat cgaagaagat gaccagacat tttgtatcaa tttattagaa gaaacaggag
     8221 tactattagt accaggaagt gcctttgatt taccgaagca tgcccgacta ggttactgtt
     8281 gtaaaaaaga aaccttggaa aagggattat tcctcttatc tgagttcttg aaaaaacagg
     8341 cctaaacaaa aacactgcaa ggacagtctg tgacaactgt tcaagcagtg tttttcattt
     8401 acgcttattg tctatgagaa ggagagtgcc ctgaagctat ggagactcct gaagcttttt
     8461 cttctgcagt ttcttgctga tggatctctt gtgtattgtg tcgtacaagc aagaagttga
     8521 ccaaaacaag aagagaagtc gaaagaaaga cgccacgata accaaatgca ctggaaacac
     8581 tggagccgat cataggccct actacgttac cagttgcttg gaaagattgg ttataactga
     8641 agatccgtcc agcagcttga tgaggcgaat attttgtgat caatgcttgg acagcaggga
     8701 gcagacaagc atcagagata ccaatcaaaa aacgtaaggc agctaattgc caaacattcg
     8761 tgacgaatgc cattgggata taaaccagaa tcgcaaaacc caaaccaatc gcaagtattc
     8821 tttcactccc aatcttgtca cctaagcgac caaatcttgg agcggctatc aaagtggcaa
     8881 tcccaggtat cgaagcaatg attccgctga tcaacgtaac attcccatgt ccatgcagca
     8941 attggcgaat gtacagactg atgattggac taatggaatt gttggatgct tggatgatca
     9001 tcgttgtgac gaacataccg atgacgacgt gtggatattt caagtctttg aaaatctgtt
     9061 tggcagaaac catgttttct tttttcactg ggacaaattt ttcatggacg aaaaacagac
     9121 tcaacaagaa gacgaaaaat aagatgatcc ctgtaatgaa aaaggtaggg cgatagccaa
     9181 acgctgaagc agagatcccg ccaagaagcg gtccaagtaa tgttcctgta acagaaccag
     9241 ttgctaaggt tccaagaact tgaccacttt tttcttttgg cgtcccagtt gcgacaaggg
     9301 cagtggcatt actgatataa ccagaaaaaa tcccttgaag cagacgcaat gcgactaatt
     9361 gatacacact agtcacgcaa cccatcaaag aaatgacgat tgccattcct aatgaagcac
     9421 gcaacagcat caattttctt cctttttggt cggctaattt tccccaccaa ggagagataa
     9481 gcgtagtcac caagaaagta gatgaaaaag tcaatccgct ccaaaagttc aattgtgacg
     9541 tactataatt acctaacgta tctatgtaaa gtgacataaa aggcatgact aaactaaatc
     9601 cgataccagc cataaatgtt ccaaaccata agacaaacaa gttttgttcc caagtttctc
     9661 ttttcctaaa taatttttcc tttattcttg cagccaatta gactccttct ttctgaaaat
     9721 tttcatctac agctctttat tataaactac aatatgtagt ttatcaatga aagtctcttt
     9781 tttctctttc tttttcgtgg taaactgaaa gagtgaagga tttattttat gagaggaaac
     9841 atggaaatga aaaaaagaaa tctgtctcaa gagaaaatca tcgactgctt tcgagaacta
     9901 gcagaagaga tggatgttca gcaaataacc tttcagcatt tagccaaacg cttggatatc
     9961 aaggctccct cgctgtataa ccatttcaaa aatattcgtg atgtcaaaac agcgctaaca
    10021 gcgaaacttc tgcaggaact taatgaccag ctgcgacggg ctcttgtggg aaaaagtggt
    10081 caggaagcac tgcagatcta tgcagaaact tatcgatcct ttgctttttc aaatcaagca
    10141 gtgtatgagt tactgatcag tgtaccgcat accaatgaag ctgttttatt agatggaatc
    10201 catgaaacga atcagatcat tttgcagata tttgaggctt ttcaattaac gaaagaagaa
    10261 aaagttcatc gaagcaggga acttcgtagt atgatccacg gttatctttc cttacgtttt
    10321 ttaggatact ttacaaaaga accaacaatc gatccagaag acagctattt ttggatgatc
    10381 aataacttta tcgctacttt gccttaaaca atcagattca tttgtaacaa ttttaagatg
    10441 ttcattaaat gaccctttta taagagaaaa ttcttgcaac tttcttgaaa agtgactaag
    10501 atgagtctag aaaagctgaa agaaggagca cgagtatgta tcaagtaatt accatgttcg
    10561 gggacaatga gccttggtgg ttttttgaag actgggaaga agatattgaa gaagaagaaa
    10621 cattcgagtc tttcgaagaa gcacgacaag cgtacgaaaa acaatggaac gaaatccatc
    10681 aaaattatga atatatcaat gcaaaaagta actttctcag cgccttttgg aatgacggag
    10741 atgagcgctg gtgtgaagag tgtgacgatg atttacagca atacaaggga cttgcgttgt
    10801 tgaaaaatca tcagcccatc acagtagaaa gcagaaagga attttatgaa acaactaatt
    10861 ctagcggaaa aaccaagcgt tgcgaaagac ctaagcaagg tgcttggtgc taatcaaaag
    10921 cacaaaaatt attatgaagg acccaaggtg atcgttactt gggcccttgg tcatttatta
    10981 ggattgaaaa tgccggaaga tctgaataag gaatggcaga gctggcaaat ggacacgttg
    11041 ccaatgatcc ctaaaaaact aggaatcaaa ccacttccta aaacaggaca tcaattaaaa
    11101 gcaatcaagc agctggctca gcgaaaagat gtctcagaag cagtcattgc gacagatgcc
    11161 ggaagagaag gggaacttgt tgctcgctgg atcttagagt atgtgcattt caataaacca
    11221 gtgaagcggt tatggatctc ttctcaaaca gataaagcaa tcaaaactgg attcaagcaa
    11281 ctaagacctg cgaaagaata tgatgcattg tatgaatctg ctcttgcccg ggcaaaagcg
    11341 gactggctgg taggattgaa tgtgaccaga gccttaactg tgaagtacgc ggataattta
    11401 tcagcaggcc gtgtgcagac accgacacta gcattagtga gagaccaaga aaagaaaatc
    11461 gagcagttcc gtccacaaac gtatttcacg atcacgttgc aagctggaaa agaacaagcc
    11521 aaaatgcttc aaaaaaatca atttgcttta aaaacacaag aagaagcaca aaaacttgtt
    11581 caattgatca gtacaagcaa aggaactgtc acagaagtcc aagaaaaatt aaaaacggaa
    11641 tctgcgccat tgccttatga tttaacagag atccaaagag aagccaatca acgctatggc
    11701 tattctgcaa agaaaacatt aagtcttgta caaagtttgt atgagaccca taagatcgtt
    11761 acttatccgc gtacagacag caaatatctg acaaatgaca tgaaaagtac gatgaaagaa
    11821 cgattgcaag cagtcagcga ctttgctcct gaggtcaagc agtatttgaa aaacggagcc
    11881 atcgtccaac agacaaaagt attccaagac agtaaagtga ccgatcacca tgcacttttg
    11941 ccaactgaaa acagagcacg ttatgaaaaa ttaagtaatg aagagcgaaa aatctatcaa
    12001 atgattgtct ctcgtttttt aggactgttt gctcaacccc ataaagtcag ccaaacaaaa
    12061 gttacggttg aatttggtaa agaacgattc atttttcggc aaaatcgtgt cgtccaagca
    12121 ggatggaaag gcgaaactga aactgagacg gaaacagtca aatgggaaaa aggaatgaga
    12181 atcaatcccg atttcgccat caaaaaagaa ctatctgcac cgcctaaacc attgtcagaa
    12241 gcaagcttgc ttggtcaaat ggaaaaatac agtctgggaa ccccagctac gcgtgcagag
    12301 attatcgaga aactaatcaa atctgaactg atggaacgta cccctcaagg attgcaagta
    12361 tctcctaaag gtaagcaatt attagtattg gtaaatccct ctttagtaac accagatttg
    12421 acagaaagct gggaaaaaga ccttgaagct attgctgcag gaaagaaaca agcaggaacc
    12481 ttcttgaatg gaatcgaaaa agaaacaaaa cgcttggtga atgagatcaa atcaagcaaa
    12541 caagaatatc aagatttctc gattacccaa aagaaatgcc cagaatgtgg ggccaatctg
    12601 cgcgagaaaa acacacgtga cggcaaaatc tatgtctgca ccaattcaga ttgcaactac
    12661 cgcagaagaa aagatccaaa agtctctaat catcgatgcc cgcaatgtca ccggaaaatg
    12721 gaaatcatcg aaggtaaaaa cggtgcttat ttccgttgta aatacgatgg aacgacagaa
    12781 aaattgcttg ataaaaaaga gcagaagaag aaaatgacca aacatgaaga acgacgcttg
    12841 ctgaaaaaat atgcacaagc agaagaacca gaagaaagcc cattagcagc agcactaaaa
    12901 gcagcaatga agaagtaata agatatgaaa gaggttgtga tagaagtggt cagcttcaag
    12961 aaataagaag gaatttacga aaattgcttc tcaaattttt gtgaaatttc ggcttatttc
    13021 cgaagaagct acttctggaa caccatttat tcggaaaaaa ggactgtgac aagacttttg
    13081 tcacagtcct ttttcattta aaaaaatgtt tacttcattt gacgtatatt attggtgagg
    13141 cttcaattca tttggcttca ctttttctgt ttctcttatg atataaacag gacgctgttt
    13201 cgtttctaag aagattttcc caatatattt cccgatgatt ccaagacata acaattggat
    13261 tccaccaatg aacaggaaga tcgagaccat ggaaggccat cctgatgtag gatcaccaaa
    13321 caacaatgcc cgcaaaataa tgaacaaaat tgcaatacca gaaccgacac aagacaaagc
    13381 gccgataaat gaagcaatcg ttaacggtgc atccgagaag ttcacgatac catcaatcga
    13441 gtagttgaat aatttccaga aggaccaaga tgtttcgcca gccacacgtt cgcggttttc
    13501 ataagaaata tattctgttt taaatccgac ccagctaaat aatccttttg aaaatcggtt
    13561 gtattctgtt aattctagga cggcatccac catttgacga gtcatcaagc ggaaatcacg
    13621 agcgccatcg accatttctg tatcgctgat tttattgatc aactggtaga attttttcgc
    13681 aaagaaactg cgtatcgcag gctcgccttc acgagtcgaa cgccgggtgc cgacacaatc
    13741 gatatcagaa gtagtgatcc ggttgatcat ctcaggaagc agttcgggcg gatcttgcag
    13801 atcaacatcc atcacggtca ctaaatctcc tgtagaagct tgcaatccag cgtacagagc
    13861 cgcttctttc ccaaaatttc tggaaaatga gacaaagcgg acataaggat aagctgaggc
    13921 tagcttcttg attgctgcta acgtaccgtc ttttgatccg tcgtcaataa acaaatattc
    13981 gaactcatgg gcacctttga atttttcaac ctcatcaaaa aaaagaggaa cagttttttc
    14041 ttcattaaaa catgggacaa caattgatac tttcatcgtt tctatccttt caatcgtatg
    14101 tttatatatg gtttacattc aaaatttaat aaaccttaaa taacaccttt aaaatttgat
    14161 tttacgtaaa agaacaggta ttctttttgt aaactaacgt atttagttta ccatgtttcc
    14221 tcggtttatc aaacttttaa gaacaatttt tattagttgg caagtagttt tggatagaga
    14281 aaaaggagga agttccagtc tattgactag gagcaaacaa aatgaaaata cacacaccta
    14341 tcggcttttt atggttagat gcagatgaat ccggattgac tgctgtatct tttcagccaa
    14401 tttcagaaag cgaatcagcg aatcagcgaa ttttaacgcg cacacaagaa cagtttgaag
    14461 aatattttgc gggaaaaaga aaagaatttg atgttccttt atctataaag agaggtacgc
    14521 cttttcagca aaatgtctgg aaagctttgt cgaccattcc ttatggagaa atccgaacgt
    14581 ataaagaagt agccgttgct gttgatagtc cgaaagctgt gcgagcgatt gggcaggcaa
    14641 atcgaaccaa tccattgcca atcgttattc cttgtcatcg agtgatcggg caaaatggaa
    14701 aactgacagg atatatggga aacgcaatag atggggttga aatcaaacgc caattgttgt
    14761 tgttagaagg atatctgcaa aattaaaaac agttgtgatt cggacattgt tccatgcgag
    14821 aatcacaact gttttttcat gttcattcta aactttgatc agccaaataa atagtaaagc
    14881 catcactgtt tggagaagcc cgacagctaa tccataagat aagaagcgtg taccttcttt
    14941 catgaatttt gcaaagtcta aacgcagacc aattgccgct aaagcagtga tctcaaacca
    15001 gctgctgata aaatgtgccg attggtcaat aactacaggt aaagaaaaca agctgttgac
    15061 caaacaaaag atcacaaacc ctaatacata ccaaggaagc ggtaattttc gttttttctg
    15121 cgtatcagtt ggcgtttcaa acagagctgg ttgtttcttg tgcatgaatt tttcaaacag
    15181 atagacaaca acaacgagca tcataatacg tagaatctta aatagcatgg cgaattcgac
    15241 aacagaattg tcaagtaagc ttgccccagc gactacttga ccaacagatt ggagtgtacc
    15301 acccaaaagt gcactttttt caatgagatt tccaccaaac aactgtaatc caagaaaagg
    15361 gagagtcaac atcatcacag tacccagtag attgactagt gtgatgatct gtcctttttc
    15421 ttcctctttc gcatggatgg aaggggcgat tgaagcaata gcagaagaac cacaaacagc
    15481 gtttcctcca gccatcatca aagaagaagt atcgctgaat tgcagttttt taccaatcaa
    15541 gtaagcaccg atgatagtga ctgacatttg cagtaacaca aaaacaattc cttttacacc
    15601 aatttgagca atcgtttgag ctgtgacagt tgcacctagt aaaaaaacag agaattctaa
    15661 tagtttgctt tcagcaaatt ttgtgccgat tgctagaaca ggctgtttaa aaaaagtatt
    15721 tcctaataaa atacccaata aaatagcaat agtcgctcca cctaactgtg gcagataaat
    15781 agcaagaagc ctgctgaagg cagcgatggc aaaagaaagc agtaaaccgg gtaagatttc
    15841 gcggaccgct gaatccttta cagattttat tggatgtgac aatgatttca tttgatcggt
    15901 tcctttcgtc aataaatact aaaagcatac tcgaacatag tagcactaaa ccagtatttt
    15961 gacaaaagaa caagaggtcg tgacaaaagt cttgtacgac ctcttgtttc gaataaacgg
    16021 tgttccagaa gtagcttcta cggaaataag ccgaaatttc acaaaaattt gagaagcaat
    16081 tttcgtaaat tccttcttat ttcttgaagc tgaccacttc ttttacgacc tcttggtaaa
    16141 cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc agtacaaaaa catgaacagc
    16201 tgtttttgtt tggccgttct tattacttga tgcagaacag ctttttcaca acctattgaa
    16261 tcgattatta agcgattttt ttagaaacaa attttccatg tttgctatgt tctttaatta
    16321 agaaacgtac gtaatgagca agaacgtaga agacaactac acttgcaaac agacattccg
    16381 caacttttaa gaaatccaat acgctactat agatacctaa actgttgaag taatgaacag
    16441 tagtaaatat agctgttgca gagataacaa gcgggaatgt aaaggctgca tagcttggat
    16501 aaaaacgaag cttgaacaaa cgtggaagca tagacagcac aacgaaatac aatacttgag
    16561 ataaagcgaa caatgctacg acaagataaa aattaggttc agcaaagttt ttaagataac
    16621 ctgttaggca aagagaacca ggtgctgcaa cgatcgtaat cagcggacgt gtcggatgag
    16681 ccatatttct tacgaaaaag acacgtttga tgatgatcgg caataagaac gcatagcaga
    16741 tcaatgaaat ccaaaagaaa atttttccaa taagtgggaa gaagttcccg gcagttacag
    16801 tgatgacccc aaatccaacg taaatgataa accagcttgg ataaattgct tcaattgaaa
    16861 gatcagcttt gataacatgg tgataattga aataaaagac aaggatcact tggaagataa
    16921 cagcgcacag ccaaatgaat ttgacaattg gtgcaatact ttcatatgaa acaaaataag
    16981 tacaaataac catgagtgac atcgtaaaag ttggtgaaac ggaggcgatg attggatcat
    17041 gcaacgtttg tttcgtgtgt tcgaataaga taaacaattt aacaaaaatg aatatcataa
    17101 gaatcataga gataccgcca gttagattgc ctaaagcaac atggtgatag tctttaaaca
    17161 gatttccaag ggaagctaac ccaaggatca acccgcagat cgggatcgga ataattttca
    17221 aaaagttctt catatataat attctccttt ttatttaata gattttagta ccaagatgaa
    17281 cgaatacatt gtaaaatgaa taggaatgta tgattacgag caagcagaca gctgattgtc
    17341 ttcatctaag ttactagttg gatcagcaac tgttttgttg tacatttcta ttatagatgg
    17401 ttttttgtat atttaaatca aatagttttt atagtaatta tcataaaaag ttatggaagt
    17461 ggtgccatgt ttaagttatt aacgactttt cgagcagtat atgaaacaag aaatttttcc
    17521 agagcagcag aagtactgtt tttatctcaa ccagcagtct cgaatcaaat caaacagttg
    17581 gagcgggaat tgaatatcga tctttttcaa cgtaacggaa gaaaagagat gcttccaact
    17641 aagcaagccg acttattata tgaacggaca ctggttttgt tagaagaatg ggaagaaact
    17701 cagcaaaaat tgctcaatga acaaaatgaa gaagaagtat gtcggatcgt tgcttcccac
    17761 acctttgctg tctatctttt gcctcagttg atgaaacatc tgatcaaaag gtttcctaaa
    17821 gtgcattttg agatcgaaat cacgaattct catgaatcat tagaacaagt agcgaaacat
    17881 gaatccgatt ttggttttat cgaaaagcca ttagagactt ccggcgtaca aagatactct
    17941 ttgatggaag accagctagt catcgctggg gacccaaaaa gcaaattgtg gcttgtaaga
    18001 gaagcaaact ctggtgtttt tcattacacg cagcgttatt ttgaagagat gaatatccaa
    18061 gataagaaga tgatcgtgaa aagtaacgat gtaattgttg cgttgctgaa ggaaggaatc
    18121 ggtcgctcga ttttgtctaa gcgctctgtt ccagaaaatg tgccaatgac tccgttgaat
    18181 ttccatcgat atttttattt catccaacga accaatctgg tttctgatca gctgctagag
    18241 attgccgaag agatccttcg atacaatgaa agcacagaaa cttctcacag cgaggagaat
    18301 ctcagctggt agataattgc atgtatcaaa aaaagttcta tgatacacta aagataagca
    18361 gtaggctttt gtactgcatt tgctcaagca actgcacaaa cggaaagtta atttttccca
    18421 aatgtctatg gaaagacttt ccattatcta actggaccgc tataataatc agcagtatgg
    18481 tttttaagaa aagggatgaa acatggaaaa aaaattaaaa gcttcgaacg ataatcgtat
    18541 tgattatggc gtgattttac cagtctttct gttatgtttg attggtctgc tctcattata
    18601 tgtagcactt agccatgatc cgaacaatcc agcagtcttg aaaggcgtag ggatgcaagt
    18661 tgtctggtat ttagttggag cggcagcaat cgttgtcatc atgcatttga attccaaatg
    18721 gatctggaaa ctgactcctt acctttatgg tttggggtta gtggtaatgg gactcttgct
    18781 caaattctat gatcagaatt tagctgcttc aacagggtct aaaaactggt ttcgattcgg
    18841 tgcgtttact ttccagccag cagagttgat gaagattgcg tacatcttga tgatggcact
    18901 gatcgtaact aaacataata cgcaagtaaa agaacggacg atgaaatcgg acttttggct
    18961 gattgggaag ctcttgatcg tgacaatacc ggttttagca ttgatcatgg cccaagatga
    19021 ctttggaacg atgcttgtct ttcttgcgat ctttggcggg attttcctga tgtctggaat
    19081 ctcttggcgc atcatcgcac ctgtagtaat cttggcagta gtcgttggag caggaacgat
    19141 cttcttggtt acgactgaag gcggaagaga cttgctttat aaagttggat tcaaatctta
    19201 tcagtttgca cgtattgatt catggctgga ccctttccat gatacgtcag gaatgagtta
    19261 tcagccggcc caaggacttt tggcaattgg taccggtggc ttgtttggta aaggattcaa
    19321 cgtaagcaat atctatgtgc ctgttcgtga gtccgatatg atttttaccg ttatcggtga
    19381 aaacttcggc tttatcggtg gtgcattcgt cattttcttg tattttattt taatctatcg
    19441 tatgattcgg gtgtgtttcg atacgaataa tgaattttac gcctatatcg caagcggttt
    19501 gatcatgatg ctgctgttcc atgtctttga gaatattgga gcaaatatcg gattacttcc
    19561 tttaacaggg atcccattgc ctttcattag tcagggagga tcctcgattt tagggaacat
    19621 gatcggtatc gggttgatat tatcgatgag ataccaaaat gaagcacctg caagatcttc
    19681 aaggaggtaa gaaaattgta caccttttac tggtacccga aatgttcaac atgcaaaaaa
    19741 gccaaagcgt ggttagacca gcatggcgtt agttatcaaa ccgttgatat gatcaaagaa
    19801 gtaccgaagg cctcaacgat tgagacttgg ctagaaacga gtcaagtacc aatccgtcgc
    19861 ctgttcaaca caagcgggat gaaatatcgg gaacttggac tgaaagatca agtaggcgag
    19921 tttacggctt caaaagcgag ccaagtctta tcgacagatg gtatgctgat caagcgacca
    19981 ctgatcttac gagaggacca gcttgttgca attggattca atgaaaaaat ttatgaagga
    20041 gaactgatat aaaatggcag gcaagcattt gaagaaaaag gacaaccttt ggatcttatt
    20101 taatggaaaa gaatactgtg ttggtttgac aaatgaagcg caagaagaac ttggagacat
    20161 cacgtttgcc aatctgccaa aagcaggaca atcgctaaaa aaagatgaac ctctgatcga
    20221 agttgaagcg gaaaaagcgg tcaatgaatt tgccagcccg ctaacgggta cagtttcttc
    20281 tgttaatgat aaaatcggtg aagatatcaa tatcttgaat gattcagatg aaatgaatgc
    20341 gtggatctta agtttcaaag atgttgatcc aaaagaattt gacgaattat aaaatcagaa
    20401 aatcacttga aaaaagttga cactgattct tccagttgct ataatcataa gcaatcctat
    20461 atgaaacgag aaagctttga aaagaagagt acatgtcacg gacgtttgaa gagagccctg
    20521 tttgctgaaa atgggtaacg aaaatgagat ggaagatggt cttagagcag agtaagcgag
    20581 cgtaagcaaa cttactacgg gagtgcccgt tacagcgcga cagtatacgg aaagaagcta
    20641 agtccaagta ctggaagagg ctgttattgc gaagtaacgg taaatcaagg tggtaacacg
    20701 gaagttcaag ctttcgtcct tttgcttaag gcaagagggc gaaggctttt ttgtttgaaa
    20761 aatcataaga gaaagatgtg aatggcatgt cattaatcca actaacgaat gtcaaaaaga
    20821 cattcaatgg gaaaaacgga aacatccaag ccgtcaatga cgtttcacta aacgttgaaa
    20881 aaggggatat ttatggaatc gtcggctatt ctggcgcagg aaaaagtaca ttagtccgtc
    20941 tcctgaatgg acttgagctt cccacgagcg gcgaagtgat cgtaaacgat caagatatta
    21001 caaaattaaa aaacaaagaa ttacgtacat tccgaaagaa aatcggtatg atcttccagc
    21061 atttcaacct gttatggtca agaactgtac ttgaaaatat ccagctgccg ctggaattag
    21121 ctggcgtacc aaagagcaaa cgtaaagaac gggcagaaga actgttgcgt ttagtcgggt
    21181 tagaaggacg cggacaagca tatccttcac aactttccgg cggacagaaa caacgcgtcg
    21241 gtatcgcacg agcattggca aatgatcctg agatcctgct ttgtgatgag gctactagcg
    21301 cattggaccc gcaaacaaca gaagaagtat tagacttatt gttagcgatc aataagaaat
    21361 tgaatttgac catcgtcttg atcacacatg aaatgaatgt gatccgcaaa atctgcaaca
    21421 aagtcgctgt gatggagttg gggaaagtcg tagaagaagg agacgtattg actgtcttca
    21481 gacaaccgaa aaaagaagta accaaacgct ttgtacaaca agacatcgaa ccagaagctg
    21541 attcgacaga actattagct gaactcatca aagaaaatcc aagcggccgt ttggcaactt
    21601 tgacctttag cgaaagaaat gccaacgagc cagtcatttc ccaagcgatt cgacgctata
    21661 acatcgatgt caacgttgtg tatggaaaga tcaaacaagt gaaagaaggt tcctttggtt
    21721 cattgactgt cttgatgact ggtgaaacca aagaattaga tcaagcagaa gtgttcatca
    21781 aagaacaagg agtaggaatt gaggtgatcc atcgtggata aaagcttttc agaaacatat
    21841 ctagatttta aacaaatcaa cccggaaacg atgaatcagg ctatccttga tacactatat
    21901 atgacattaa tctcaatggt cttagtgacg atcatcggat tagttttggg attgatcctt
    21961 tactctattg gacgcaaaaa caaaccgtat gctcgaatca tttatggaat cgtatcgatc
    22021 atcagtaata ttttccggtc aacgccattt atgatcctga tggttttgat cattccattt
    22081 actaaagcaa tcgtaggcac tatgctaggt gcgcaagcag ccattccagc tcttgtttta
    22141 tccgcagcac cattttatgc ccgtctagta gagattgctt ttcgtgaagt cagccctggt
    22201 gtactggaag cagcagatgc aatgggagca agttactggg aaatcatctg gaaggtcttg
    22261 atacctgaaa gtattcccgc attgatttct ggattaacag ttacaacgat ctcgatgatc
    22321 ggatttactg cgatggccgg cgcgatcggc gcaggtggac ttggaggtct tgcttggcag
    22381 gaaggctacc agcgtggtaa tctaacagtg acattcgttg cgactttaat tattttacta
    22441 atcgtattta tcgttcaagg aatcggtgat ttcttaacta agaaaacaga caaaagataa
    22501 taggatatag ggggatgtag gatatgaaaa agaaaatttt tggatttgca gcagcgcttt
    22561 tgctgactgt aggattagca gcatgcggta acaacagcga cagcaaagat tcagcaaata
    22621 aagcagacga cacgacattg aaagtcggcg cttcaccgac accgcacgca gagattttgg
    22681 aacatgtgaa accattattg aaagatgaag gaatcgatct tgaaatcgtc aaattcgatg
    22741 actatgtatt accgaaccaa tctttggaag aaggcgacat tgatgccaat tacttccagc
    22801 atattcctta tttgaacaaa gaaatcaaag aaaaaggata tgactttgtc aatgccggtg
    22861 ctgtccacat cgaaccaatg ggcttgtact ctaaaaaagt caaagatgtt tccgaattaa
    22921 aagatggtgc aacaatcatt acttcaaatt ctgaatcaga ctggggacgc atcatcacaa
    22981 ttctacaaga tgctggctta gtcaaagtca aagatggagt agatctggaa acagcaacat
    23041 tcgatgatat tgaagaaaac cctaagaact tgaagttcaa ccatacgatc gacccatcac
    23101 ttttagcttc tacgtaccaa aatgatgaag gtgacctagt tgcaatcaac gcaaacttcg
    23161 cttacggcgc agggttgaat ccattagaag atgccgtatt gcttgaaaag gacaactctc
    23221 cttacgtgaa tattattgcg actcgtaaag gtgatgaaga cagtgacaaa atcaaaaaac
    23281 tgattgaagt gcttcaccgc aaagaagtcc aagaatggat cacagacaaa tggggcggct
    23341 ctgttaaacc agtaaaggct gatgcaaaat aaggtatgga acaaaaaaga tgagaaataa
    23401 gtctgtaatg gcttatttct catctttttt atacctattc taatttagaa atcaaaattg
    23461 tttaataata aataaaagat atttgatttt agtaaggaag gtcattgcgc ttcattctca
    23521 attagtctaa aatgagtgag aacgaaagag aatttggggg tataatgcat gtctgtctta
    23581 gaaataaaaa atctacatgt gtcgatcgag gaaaaagaaa tccttaaagg cgtaaaccta
    23641 acaatgaaaa ccggagaaat ccatgccatc atgggaccta acggcacagg aaaatctacc
    23701 ttatctgccg ctatcatggg caatccaaac tatgaagtaa cagaaggcga aattttgttt
    23761 gacgggaaaa atgttttaga cttggaagta gatgaacgtg cgcgacttgg cttattttta
    23821 gctatgcaat atccaagtga gataccaggt atcacgaatg cggaattcat gcgtgcagcg
    23881 atcaatgcga aacgtgatga aaacgacaaa atttctgtta tggcattttt gaaaaagtta
    23941 gatgaaaaaa tggctttact aaatatgcca gaagaaatgg ctgaacgtta tctgaacgaa
    24001 ggtttttctg gcggagaaaa gaaacggaat gagatcctgc aattgttgat gttagaacct
    24061 acatttgcaa ttttagacga aatcgattca ggtctggata tcgatgcttt gaaagtcgtt
    24121 gctaaaggag taaatgaaat gcgcggagac aatttcggtg cattgatcat cacccactat
    24181 caacgattat tgaactatat cacacctgat gtcgttcaca tcatgatgga aggccgagta
    24241 gtcatgacag ggaatgctga cctagctaaa cgtctggaag cagaaggcta cgctggtatc
    24301 agtaaagaat taggaatcga ctataaagaa gaagaagcgt aagggggagc aagaatgaaa
    24361 gaaaaaaact ggctcgatta tcttgacgcg gtaaatgatt tttctttaag taaaggcgaa
    24421 cctgattgga tgagaacctt ccgtcaagac gcactagcga aagcagatga actgccattg
    24481 ccgcatatcg atcgggtgaa attccatcgc tggtcgttat ttgatgtgaa agaaacacaa
    24541 accatcagtg aaacaggcac gattccggca tttgatgcaa tgaaagacaa tcctgtgctt
    24601 gtccaacaag gttcatggac gatttttgaa caactgcctg tggaattagc agaaaaagga
    24661 gtcattttta ccgacttgtt cactgcaatg atcgaatatc cagaactagt ccaagaatat
    24721 tatatgaaaa aagcagtaaa catgaacgaa gaccaattga ctgcactcca tgtagctttt
    24781 atgaacagcg gtattttcct gtatgtgcct aaaaatgtcg tgatcgatga accactcgag
    24841 tccttattca ttcaagatgg cgcatccgat gagcacttct tcaagcatgt gttgatcgtg
    24901 gcagatgaac atagtgaatt ttcttatctg gaacgtttcc agacaaccaa agaacaagta
    24961 gcaaaatcat caggtaatat catcgtggaa gtcatcgcta aagctggttc aaaaatcaaa
    25021 tattcagccg tagatcaatt aggagaaaat attacttctt atatgaatcg ccgtgggcac
    25081 attttacgag atgcttctgt cgactgggca attggtgtca tgaatgacgg tcatgtgatc
    25141 gctgactttg actcagattt agcaggagaa ggtgcacacg cagaagtcaa aatcgtggca
    25201 atcagctcgg gcagacaaat ccaaggaatc gatacacgag taaccaataa agcccctcat
    25261 acgatcgggc atattttgca acacggagtg atccgagaaa aaggaacatt gacctttaat
    25321 ggtatcggcc acatcttgaa aggggcaaaa ggtgcagatg cccaacaaga aagtcgtgtc
    25381 ttgatgcttt ctgataaagc acgtggtgat gcgaatccta ttcttttgat cgatgaaaac
    25441 gaagtaactg ctggacatgc tgccagtgta ggacgagttg atccagaaga aatgtactat
    25501 ctgatgagta gaggattgca taaagaagaa gcagagcgct tagtgattcg aggattcctc
    25561 ggttctgtct taacagcaat ccctgtagaa caagttcgga aagaattagt cgaagtaatc
    25621 gaagggaagt tgaatggatg atcgatgcta aaaagctacg caatgatttt cctatcttat
    25681 ttcaagacgt caatgatgaa cctttagttt atctggataa tgcagcgacg acccaaaagc
    25741 caaaacaagt actccaggca ttagcgtact attacgaaca cgataacgcc aatgttcatc
    25801 gcggtgtcca tacgttagca gaacgcgcga cccatgatta tgaacaagca agagaaaaag
    25861 tacgggcgtt catccatgca aaagaaacag cagaagtgct gtttactcgt ggtacgacga
    25921 caagcttgaa ttgggtagcc agaaccttcg gcgaccgcta tatcaaagca ggagacgaga
    25981 tagtgctctc ttacatggaa catcattcta atgttatccc gtggcagcag ttggcaaaac
    26041 gaacaggtgc tgtcttgaaa tatatcgaac taacagaaga aggcttcctg gatatggagg
    26101 atgcagcgaa aaagattacg gacaaaacca aaatcgtctc cattgctcat gtatccaatg
    26161 tcttaggtgt cttcaatcct gttcaagaat tagctgaact tgtccatgaa aacggcgggg
    26221 tcatcgtagt agatggtgct caatctgccc cgcacatggc cattgatgtc caagcactag
    26281 actgtgattt ctttgctttc agcggacaca aaatgtgcgg accaacaggt attggtgttt
    26341 tatatggtaa acgaaaatgg ttagaagaaa tggaacctgt tgaatttggt ggcgaaatga
    26401 tcgattttgt cgaattatac gacagtacat ggaaagaact accttggaaa ttcgaagcag
    26461 ggacaccaaa tatcgccggc gctatcgcat taggccatgc aatcgattat ttgcaaaaac
    26521 tcggtatgga caatatccat cgatatgaag aagaactagc cgcttatgtg ttgccaaaat
    26581 tacaagcaat tgatggattg actgtttatg gtccacaaga cccgaatttt cggacaggag
    26641 tcgttgcatt caatttggat gggttacatc cccatgatgt cgcgacagct ttagacatgg
    26701 aaggggtagc cgtcagagca ggtcatcatt gtgcgcaacc gctattgaag taccttgaag
    26761 tatcagcgac tgctcgcgcg agcttttatt tctacaacac aacgcaagac gccgaccgtt
    26821 tagttgaagc aatcatggca acaaaggagt ttttccaaca tgggactatc taaattagac
    26881 aatttatacc gtcaagtgat cttggatcat tcttcccatc cgcatcatcg tggaagcttg
    26941 actgactcga caagcaagat cgaattgaac aaccccacat gtggagatgt gatcgaattg
    27001 caagtcgatg taaaagacgg aatcatcaaa aatatcgcct ttgacggcag tggctgctcc
    27061 atcagtacgg caagtgcctc gatgatgaca gacgccgtca tcggaaaatc aatagaagaa
    27121 gcagaacatt taactgtaga ctttctacag cttgtccaag gaaaagaagt caaagatccc
    27181 gaccaattag gcgatgctgc tatgcttggt ggtgtagcta aatttcccgc tcggatcaaa
    27241 tgtgcgacac ttgcctggaa agcactagga aaagcaatgg aaaacgatgg taccgcagct
    27301 ttccatcatt accacaagga agaggagtga gaacatgggc gtaccagaat tagaagaata
    27361 taaattcggg tttcacgatg atgtcgaacc ggttttcagt acaggagaag gcttgacaga
    27421 agaagtgatc cgtgagatgt caagaatcaa aggcgaacca gaatggatgc ttgaattccg
    27481 tctgaaatct ctagaaacat ttaacaaaat gccaatgcaa acatggggcc ccgacctttc
    27541 agacatcgat ttcgatgcca tcaaatatta ccaaaaacca agtgacaaac ccgctcgtga
    27601 ctgggaagac gtaccagaaa aaatcaaaga tacatttgaa aagattggta taccagaagc
    27661 tgaacgagct tatctagccg gtgcttccgc acaatatgaa tcagaagtcg tgtaccataa
    27721 tatgaaagat gaattcgaaa agttaggaat cgtctttact gacactgact ctgctttgaa
    27781 agaataccct gaattattca aagagtactt ttcaaaactt gtaccgccaa ccgacaacaa
    27841 attagcagcg ctgaattcag ccgtatggtc aggtgggacc tttatttacg tgccaaaagg
    27901 cgtcaaagtc gatgttccat tacaaacgta cttccggatc aatgcggaaa acacggggca
    27961 attcgaacgt acgctgatca ttgttgacga aggagcaagc atccactatg tcgaaggttg
    28021 tacggcacct acttattcaa gtaacagcct gcatgccgcc atcgtagaaa tctttacaag
    28081 aaaagatgcg tattgccgtt acacaacgat ccaaaactgg tcagacaatg tgtacaactt
    28141 agtaaccaaa cgagcaaaag catacgaagg cgcaaccgtt gaatggatcg acggaaacct
    28201 aggtgcaaaa acaacgatga aatacccaag tgtttactta gatggaaaag gtgcacgagg
    28261 caccatgctc tccatcgcct ttgcaggagc aaaccaaatc caagatacgg gtgcaaaaat
    28321 gatccacaat gcaccaaata catctagctc tatcgtctct aaatcaattt caaaagacgg
    28381 cggagaagta aactatcgag gacaagtaac ctttgccaaa aacagtgcgg gttcgatctc
    28441 tcatatcgaa tgtgacacga tcatcatgga cgaatggtcg aaatccgata cgatcccatt
    28501 caatgagatc cacaacagcc aagtctcttt agaacacgaa gccaaagtct caaaaatctc
    28561 agaagaacaa ctctactatc tgatgagccg aggcctgacc gaatctgaag caactgaaat
    28621 gatcgtcatg ggctttgttg aaccattcac caaagaactt ccaatggaat atgcagtcga
    28681 actcaatcga ttgatcagtt atgagatgga aggcagtgtt gggtaaacct gttataggag
    28741 ctagaaccct tgatatataa gggttctagc ttttttgttt tgcctgtata ctagaaatga
    28801 ccagattttg accagctctt ttactgatga gccatcatat cagcaaagtc ttgacctgtt
    28861 ttcttaggtg cattatttgt aatatgtgca tagatgtcta atgtcacttt actattttca
    28921 tgccctaatc gttgttgaac taccttgaca gattctcctg cttctaataa taaactagca
    28981 tgtgtatgtc taaacccatg aggtgtaata cgaggcaagt tatatttttt caaaatatat
    29041 cttaaccaat cattaacagt gtttggaatg tatagagtgt ttttaactgt tgtaaaaaca
    29101 tgttgctctt tagagctagt attgtatcca agttttaaat actcttcttt ttgctgtaat
    29161 ttccagttgt tgaggataga aagagtctca gtatctaaag agataactcg tctagaagct
    29221 cttgttttcg gagattgaat tataacttta ccaaattcat ctacagcaag agttttattg
    29281 acatgtagtt ctttattaaa cgtgtctatg tctttccact gtaaagcgag tacttcactc
    29341 tttctcatac ctgtgtatgc caataatctg aagaaggcgt attgtttcat gttaccaaaa
    29401 tctttaaaag catccagtaa attattaagc tcctcttttg agtaaaattt acgatttttc
    29461 tcatactctt tttttcgtgg aagaagtgtt ttttccatag gattattgtc aatatattgc
    29521 attgcaaccc cgtagcgtaa aatttgtgca gtctgttttc tcatataatc gtaactttca
    29581 tatttactgt gccattcatc taccactttt tgacaatatg ggacagagat attcgatagt
    29641 ttaagcttac caaatgctgg aagtatttga ttatttgcgt atcttactga gattgcgatt
    29701 gttgaagctt taacactggt acgctgatga gttatccatt cattatacag gtcttcaaat
    29761 gtcatatcag gtgctttctt tttaacaccg tataatattt gatgtctgta ctcgtcataa
    29821 tctttttgag cttcttgttt gctactaaaa cctcttctcg tagtatttat tttttcacca
    29881 gttcgtggat cggtggcaat gtgtccttta tacatccacg ccacttgacc gtttttcttt
    29941 ttgtactttg atatttttgc cattaaatca tctcctctta gtttacaagg agtacttacc
    30001 actagatggt atcacctccc ttcatttttt tcttagtgta ttttttccca attgatgaaa
    30061 tcgtttgttc tagcttgttt atattatcag taatttctct cacagcttct tcgggataag
    30121 acatgtcttt caattgaata ggtagccatt catctaccat tctttctagt agttcttcta
    30181 tactgctagt gaggaaaaga atgttattgt caaattcttc tacaatagca ttgatgaata
    30241 attcttctat ttttaagata ttgaaaagac taattttttc atttttaact ttattacaag
    30301 tcttgttagc aataaattct tgcatctctg gttctaattt ggaatatcta tccagccatg
    30361 tatctaaatt agagtgtact ggtgggaact gttgacgaag atgctggaac aggtttttat
    30421 ctggaaatgt tataccatct agtacaatgc tatctctata aagaataatt cccaaaatat
    30481 attcttctac attgccacaa actaactcct ctatactcat atttcccaaa tcagcaattc
    30541 gggataatcg ctctggtatt ggtaaatagg ttcccttctc ccattcgtag atagtttttt
    30601 tgtcaattaa ttgaccgttg ttaatcataa gaccaaatgc tggttgtgtt aattttttac
    30661 tctttcttat agcttttatt tttgttccga gtatcttttt tcgttctaaa taatttattg
    30721 gtggacgttt tatcatattg accacacctc ctaaaattag tatacctcaa aaaaggggtg
    30781 ttgtaaataa attttacaaa gctgttgaca cccctttttt atcgaggtat tatgtgtatg
    30841 tcgatgaact ggtaatcatt ttttgtttga ttaatcggtt aataacaaaa tacttttatt
    30901 ttgagcaaaa aatacgaaga taattcttag aaaatttgtt caagcaatca cgaaaggagg
    30961 gggaaatatg tctctacaaa tcattgatag cattgtttct atacaagttg aacaacaagt
    31021 gaaagagata attacagtgc taaagcctat gctagaagac attatttctg gagtcactct
    31081 agagtatatg gactatcagc aggcaagtga atatctaggt gtatcagtag gaacgattag
    31141 aaaatatgtt tctcaatacg ggttacctgt tataaaaatt g
//
LOCUS       DACDEG010000022        30212 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.022, whole genome shotgun sequence.
ACCESSION   DACDEG010000022 DACDEG010000000
VERSION     DACDEG010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.022"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..203)
                     /locus_tag="IV142_001106"
     CDS             complement(<1..203)
                     /locus_tag="IV142_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484777.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRI"
     gene            complement(191..820)
                     /locus_tag="IV142_001107"
     CDS             complement(191..820)
                     /locus_tag="IV142_001107"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP8484778.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(827..1543)
                     /gene="pyrF"
                     /locus_tag="IV142_001108"
     CDS             complement(827..1543)
                     /gene="pyrF"
                     /locus_tag="IV142_001108"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP8484779.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(1540..2472)
                     /locus_tag="IV142_001109"
     CDS             complement(1540..2472)
                     /locus_tag="IV142_001109"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP8484780.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(2469..3245)
                     /locus_tag="IV142_001110"
     CDS             complement(2469..3245)
                     /locus_tag="IV142_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP8484781.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(3299..6484)
                     /gene="carB"
                     /locus_tag="IV142_001111"
     CDS             complement(3299..6484)
                     /gene="carB"
                     /locus_tag="IV142_001111"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP8484782.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(6489..7565)
                     /gene="carA"
                     /locus_tag="IV142_001112"
     CDS             complement(6489..7565)
                     /gene="carA"
                     /locus_tag="IV142_001112"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP8484783.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(7570..8850)
                     /locus_tag="IV142_001113"
     CDS             complement(7570..8850)
                     /locus_tag="IV142_001113"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP8484784.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(8869..9795)
                     /locus_tag="IV142_001114"
     CDS             complement(8869..9795)
                     /locus_tag="IV142_001114"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP8484785.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(9808..11091)
                     /locus_tag="IV142_001115"
     CDS             complement(9808..11091)
                     /locus_tag="IV142_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP8484786.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(11105..11644)
                     /gene="pyrR"
                     /locus_tag="IV142_001116"
     CDS             complement(11105..11644)
                     /gene="pyrR"
                     /locus_tag="IV142_001116"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP8484787.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(11879..12790)
                     /locus_tag="IV142_001117"
     CDS             complement(11879..12790)
                     /locus_tag="IV142_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8484788.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(12787..13251)
                     /locus_tag="IV142_001118"
     CDS             complement(12787..13251)
                     /locus_tag="IV142_001118"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP8484789.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(13256..13954)
                     /locus_tag="IV142_001119"
     CDS             complement(13256..13954)
                     /locus_tag="IV142_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8484790.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(13941..14327)
                     /locus_tag="IV142_001120"
     CDS             complement(13941..14327)
                     /locus_tag="IV142_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484791.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(14474..16144)
                     /locus_tag="IV142_001121"
     CDS             complement(14474..16144)
                     /locus_tag="IV142_001121"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP8484792.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            16327..16560
                     /locus_tag="IV142_001122"
     CDS             16327..16560
                     /locus_tag="IV142_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP8484793.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(16659..17090)
                     /locus_tag="IV142_001123"
     CDS             complement(16659..17090)
                     /locus_tag="IV142_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP8484794.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            17169..17582
                     /locus_tag="IV142_001124"
     CDS             17169..17582
                     /locus_tag="IV142_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP8484795.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(17620..18768)
                     /locus_tag="IV142_001125"
     CDS             complement(17620..18768)
                     /locus_tag="IV142_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP8484796.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(18890..19486)
                     /locus_tag="IV142_001126"
     CDS             complement(18890..19486)
                     /locus_tag="IV142_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8484797.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(19686..22328)
                     /gene="alaS"
                     /locus_tag="IV142_001127"
     CDS             complement(19686..22328)
                     /gene="alaS"
                     /locus_tag="IV142_001127"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP8484798.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            22716..23147
                     /locus_tag="IV142_001128"
     CDS             22716..23147
                     /locus_tag="IV142_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8484799.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(23192..24541)
                     /locus_tag="IV142_001129"
     CDS             complement(23192..24541)
                     /locus_tag="IV142_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8484800.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(24611..25774)
                     /locus_tag="IV142_001130"
     CDS             complement(24611..25774)
                     /locus_tag="IV142_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484801.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            25903..26226
                     /locus_tag="IV142_001131"
     CDS             25903..26226
                     /locus_tag="IV142_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484802.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(26409..27758)
                     /locus_tag="IV142_001132"
     CDS             complement(26409..27758)
                     /locus_tag="IV142_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8484803.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(27827..28780)
                     /locus_tag="IV142_001133"
     CDS             complement(27827..28780)
                     /locus_tag="IV142_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP8484804.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(28882..30072)
                     /locus_tag="IV142_001134"
     CDS             complement(28882..30072)
                     /locus_tag="IV142_001134"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP8484805.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         8471 a         6644 c         5318 g         9779 t
ORIGIN      
        1 attattctct tctttctgct aaccagtctt agaaaaatga attttaaaaa aatcaaaaac
       61 tgatcacgat agaagtttct tttcatacct gatgaaaaga tgcttctcgc aatcagtttt
      121 tggatttttg ctattccaat tgatgattct ggagacacaa atcggatttc tctatcttca
      181 tcaagcgcgc ttaaatctgc caattttcag gatctttttt ccattctttt agcagagtta
      241 attcttgctc atcgatataa cgatcttcta aagctgcttc aatcaataca gaataattgg
      301 tcaacgtcat aagagggatt tcggcttttt cgaaatttgc ttttcctttt ggcaactcat
      361 acgtaaagat agccgcaaca ccaaggatat ccgcaccttc tctttttgct gcttcagctg
      421 cttccaagac acttccgcct gtagaaatca aatcctcgat cacaaccatc ttctgacctt
      481 caaatatacg tccttcgatt tggttacctt tcccatgatc tttcgcctta cttcgaatat
      541 agaccattgg caaatccagt atttccgcta cccatgcggc atgagggatt cctgctgttg
      601 cagttccagc aatcacttgt acttcagggt aagcttcttt gattttgctt gctaatcctt
      661 ttgcgatctc ttttcggact tttggatagc tcatcgtgat tcggttatca cagtaaattg
      721 ggcttttgat tccacttgcc catgtaaaag gattacttgg atttaaaaat actgcttcaa
      781 tttctaataa gtcttttgcg atactttgtt caacattcat tttaggttag ctccattctg
      841 tttgaatcgt ttgatatgct ttataaggat tagttgactg agtgattggt cgacctataa
      901 cgataaaagt tgagccgatc ttttttgcct gagaaggtgt gactactctt gtctgatctc
      961 ctgcgactgt tccttctggt cttatacccg gagttaagca aacaaattcg tctcctgcca
     1021 tttctttgat cgcttctact tcccaagcag aagaaaccac accatctaat cctgcttttt
     1081 tggcacagct agcataatgg atcacacttt tttctaaaga aacttcgatt agttgatcgg
     1141 catgcatttc ttcttcactt gtagaagtaa gctgagtgat agctagaagt agcggacgtt
     1201 ttccaccttc tggagtcccc tcttctaatc ctcgcattgc cgcctccatc atacgaatac
     1261 cgccagcggc atgaacacag gtgagatcaa caccaagctt tgccaatccc cgcattgctt
     1321 tttcgaccgt attcggaata tcgtgaagtt tcagatctaa aaatacatta tgaccagcct
     1381 cttttagata acgtacgatt tcaggtcctt cttggtagaa caattccatt ccgattttca
     1441 caaacaattt ttcttctttc gggaaaaatc gaagaaagtc ttctacctct tgcctagttg
     1501 aaaaatctaa tgcaataatt ggtcgttggt ctagttgatt catcgttcac gttcctctct
     1561 cacttctttg attaatcttt ccagcgattc gatgcctagt tcgctcattc gcacagggag
     1621 ctctttaatt agtttaggac agatatacgg atcggtgaaa ttcgcggtcc ctatagctac
     1681 agcacttgct ccagccataa acatctcaag aacatcatcc actgtctgca cgccgcccat
     1741 cccaatgatt ggaaggctgg atatacttgc tacttggtga atcaaacgaa tagcgacagg
     1801 cttgattgct ggtccggaga gtccacctgt ttgattggct aaaatcggtt ttcttgtctt
     1861 aaggtcaatc cgcataccaa gaagagtatt gatcatcgta aacccatctg cccctccagc
     1921 ttcgattgcc tgagcgattg gaacgatgtc tgttacattt ggggacaatt tgacatagac
     1981 aggaacagaa gaaactttct ttactgcttg tgttaattga aaagcaacat cagaatctgt
     2041 tccaaaagca attccgccat gtttgacatt tgggcaagaa atgttcagct cgattgcttt
     2101 cacatttggt gcttcgccaa ttttgctaca aacttctaca tagtcttctt cacaagcacc
     2161 agcaacattt gcgatgatgg gtaactcatt gtactcctct aattctggaa gaagtttatg
     2221 cataacaacg tctagaccag gattttgcaa accaattgca ttcaacatgc cagatggtgt
     2281 ttccgccact cttggcgtgg gattgccaaa gcgggcattt ggagtcgttg ccttgaccat
     2341 gatcgatcca agctgattca aatcatagta ttttccgtat tctttgccaa aaccgaaaca
     2401 gccgcttgcc ggcatgatag gattttttaa ttctaggcca ggaagtttga ccgctaaagg
     2461 attctcattc atataattac ctctcctatt ttaaaaatcg gtccttcgtc acaaactttc
     2521 acactttttg ttcctgtttc atcttccggc gtatgacaaa cgcaagcata gcaagcccct
     2581 atcccacatg ccatgcggga ttctaatgat aactggacat tttgatgcga agaaaacagc
     2641 tgttccactg tcttcaataa cccgtttgaa ccacatgcaa aaacagctgt aggttccgtc
     2701 ttcgttccta acaataaatt accgacattt ccgtgcatac cgtatgttcc atcgtcagta
     2761 gcgattcttg tttctcctag acgattaaat tctttttcat aataaacgac ttcatgtgtg
     2821 gcaaaaccta aaaaatgcgt tggcttaatt cctttttcga ctaactgttt tgataactca
     2881 tatagaggag ggacaccgat tcctccaccg attataaata cttcgtctcc tgtatgaaga
     2941 ttggacaaat caaaaccatt gcctaaagga cccatgacat ctaaatggtc tccggcagaa
     3001 agagtagaga agactctagt cccctcacct tccacccgat aaatgatacg gcaagtattt
     3061 gtttcgtggt cgatatgatt caagctaatc ggtcggcgta atagcaagtc tgcacgcggc
     3121 acgagaatat gcaagaactg tcctggttgc tgcatttctt ttactagatc acctttcaat
     3181 gtcatttcaa aaatctttgg tgccagctgt ttttgctgaa cgatttccat catttcttgt
     3241 ttcatctgtc ccctcctgca acggtttttc gtctttttgt cttaacatgt gctcgccgtt
     3301 aaatagggtt agttgaaaaa gcacgggatt ccatcactcg aacgatcgca tctgctgtgt
     3361 ctaaagaagt aaacaatgga ataccatgtt caactgcttc tcttcggatg atgaaaccgt
     3421 ctttagaagc atttccccga tctttatcaa tcgtattgac aaccacttgt gttttaccat
     3481 ctcttattaa gtcgactaca tttttttctg ctgcttcctc agaaattttc gctactgttt
     3541 caacgaccag accatttttt tcgaaataag cagctgtatg tttcgttcct atcagactat
     3601 agccaatttc tgtaaaacgt ttagctaatt ctagtgcttc ttcttttgtt tcgtccgcaa
     3661 tcgtaaataa tacggctcca tattcaggca agtgtaatcc agaagcttcg aatgccttgt
     3721 acaaagcttt gtctaattgt ctgtcagatc ccattacttc tccagtcgat ttcatttcag
     3781 gccctaagta agtatcaact ttctgcaatt tcgtaaaaga aaagacaggt gctttaacat
     3841 gaacttcttt tgtttctgga taaagtccgt tttcataacc aagtgtcttt agtttctctc
     3901 ccaaaatagc tttcgtcgca atttgtgcca ttggaattcc tgtcacctta cttaagaatg
     3961 gaacagtccg acttgctcga ggattgactt cgatcacata aacctcattt tcatggatga
     4021 caaactggat attcatcatc ccgacacaat tcaaacctaa tgctaagcgg atcgtgtaat
     4081 cttcgatcgt tttcttgatc gattcagata atgtttgagg tggatacaca gccattgaat
     4141 cccctgaatg gacaccagca cgctcaatat gctccatgat ccctgggatg agtactgttt
     4201 caccgtcaca aatcgcatcc acttcgcatt caccaccaag caagtaacgg tccactagta
     4261 ctggatgttc tggagatgct ttcacagcat gttccatgta atcttctaaa tctcgctggt
     4321 tttcaacgat ttccatcgca cgtccgccta atacatagct tggacgaaca agaactggat
     4381 aaccaattct cgaagcgata accacggctt cttctttact tgtagcagta tcccctaaag
     4441 gttgaggaat atccaactct ttcagcgctt gctcgaataa atcacgattt tccgcacgat
     4501 ccaagtcttc gatcgttgtc cctaagattt ttacaccagc agcagtcaaa ggttcggcta
     4561 agttgattgc tgtctgacca ccaaattgaa cgattactcc tatcggctgt tctagctcga
     4621 tcacattcat cacatcttcg aaggtcaatg gctcaaaata taatttatcc gaaatagaaa
     4681 agtcagtcga aactgtttca ggattgctgt tcatgatgat tgcttcgtat cctgcctgct
     4741 ggattgcttt gacagaatgc acagtcgcat agtcaaattc aaccccttgt ccgatacgga
     4801 taggaccaga tcccagtact agaattgagg gcttttctga tcgtgaactt tcattttcat
     4861 attcatagga actataaaaa taaggtgttt ttgattcgaa ttccgctgca caagtgtcaa
     4921 ccatcttata tacaggaatc aaatcatttt cttttctcat ctggcgaacg gcttcttcgt
     4981 ttatttccca cagctccgcc acttttttgt ctgtaaaacc gttttgtttc gctgttttca
     5041 atatcttgtc atccaacggc tgctgtttca actgctgttc gatttctacg atatgcaaca
     5101 gtttatctaa aaagaataaa tcgatttttg tcaaatccgc taattcttct atggtgtacc
     5161 ctcttctaat agcttcagct acataaaaca ggcgatcatc ttgtgctcga accacttttt
     5221 ggattaggtc atcgtccgtg atgtctgcca actcaggaag ttcgatatgg tgtgtaccga
     5281 tttctaaaga acgcacagct ttcaataaag actcttcgat attccgaccg atagccatca
     5341 cttcaccagt tgctttcatc tgcgtgccta aaagccgttc tccgttttca aacttatcaa
     5401 acggccagcg aggaatttta gctacaacat aatctaatgc aggttcaaat tctgcaaaag
     5461 tcgttcctgt aacaggattt ttcatttcgt ctaatgttaa tccaagcgct attttagcag
     5521 ctagcttagc gatcggatag ccagtcgcct tacttgctaa cgcagatgaa cgagaaacac
     5581 gcggattgac ttcaatcaca taataattaa aactatgtgg atcaagagcg agttggacat
     5641 tacagcctcc ctcgatctta agtgctcgaa taatcgaaag agaagcatca cgaagcattt
     5701 gatattcatg gtcagacaat gtttgactag gggcaaatac gatagaatca cctgtatgga
     5761 tacccaccgg gtcaaaattt tccatattgc agacaacgat tgcattatca gccgaatctc
     5821 tcatcacttc atattcaatt tctttaaaac ctgcaatact tcgttcaatc aaacattggg
     5881 tagcggggga aagacttaat ccattttctg atacgatacg taattcttct tcattttcac
     5941 acatacctcc accagttccg cctaaagtaa aggctggacg aacgatgaca ggatagccga
     6001 ttttatttgc aaaatctaca gcttcttcta ctgtattgac gatttcagac tcggggattg
     6061 gctgattcaa ttctttcatc aactgtttga atagatcacg atcttctgct tgatcgatcg
     6121 cagaaagctt tgttcctaga agttctatgt tcaattcttc taaaatacct gatgctgcca
     6181 gctccattgc catattgagg cctgtctgtc cgcctaaagt tggaaggaga gcatctggac
     6241 gctcttttcg aagaatcctt gatacaaatt caagtgtgat cggttcgata tatacttgat
     6301 ctgccacttc tttatccgtc atgatcgtcg caggattaga atttactaaa accacttcat
     6361 atccttcttc ttttaaggca aggcaggctt gtgttcctgc gtagtcaaat tctgcggctt
     6421 gaccaatgat gatcggacca gatccaatca ccataatttt cttgatatct gtacgtttcg
     6481 gcatctgttt actgctcctt tcctgcgtcc atcatttcca taaattcatc aaataaatgt
     6541 agtgcatcat gcggaccagg ggcagcatct ggatgaaact gcacactgaa agcgggataa
     6601 tctctgtgtt tgactccttc tactgttccg tcatttactt ctacatgagt tacgatgagt
     6661 ttttctggat caatcgtggc ttcatcgact gcatagccat gattttgaga agtaaaatca
     6721 attcttccgg tcgctacttc tcgtaccggg tgatttaatc ctcgatgtcc gaatttcatt
     6781 ttataggtat ctgcaccgtt ggctaaagca aataactggt gaccgagaca aataccaaaa
     6841 atcggtactt ttccttggat ttttttgatc atttcgattg cttctggcac atcttttgga
     6901 tcaccaggtc cattcgtcag catgactccg tctggtgata gcgctaaaat cgtctcggca
     6961 tctgtgttat agggaagtac tgtgaggtta cagtttcttt tactcaattc tcgtaaaata
     7021 ctatgtttca agccgaagtc aatgacaatg actttccgac ctgttcctgg acttggataa
     7081 ggtttgttag ttgagacttg ctgcacttga ttggttgcta gaacggttgc tttcagctga
     7141 tcaaatgcat gttcgaagga atctccagca tcaactatac tagctttcat cgttccgact
     7201 tcacggattt tgcgagtaag ggcgcgcgta tcgatacctg atattccagg aattcctttt
     7261 tgtttcaaaa atgaatctag cgtcatttgg tttcgccagt ttgaagctac acgtgcatgt
     7321 tcttttacta ctactccttt gcatgttgga gaaatcgatt cataatcatc tctatttacc
     7381 ccataattac ccaccatagg ataggtaaat gtaatgattt gaccgttaaa actttgatca
     7441 gtgatagttt cttgataacc agtcatactt gttgtaaaaa cgatttctcc gaagacacta
     7501 gcatctgcac caaaagcctt tccttcgaaa actgttccat cttctaatat aagcaaacgt
     7561 tccatacttt tattctccct tctgccacac aagttttccg ttgacaaaag tcatttgtgt
     7621 ttcgcctttt actttccagc cgataaaagg tgtattctcc ccttttgata aaaagtcttc
     7681 tttatcgatc gtgcaagttt gtgtaatatc aaaaactgct acatcagcgg gggcaccaac
     7741 cgttagcgtt cctgcattca aaccaaaaat ctcagccggt ttaactgcca accaattgat
     7801 cacttgttcc aacgtgaatc gaccagtttc tacaaaatga gtatagatca gttgaaacgc
     7861 tgtttcgctt cctacgatac caaatggtga tttcatgaaa ctttggcttt tttcttctaa
     7921 tccatggggg gcatggtctg tagcgatgca atcgatcgta ccgtcaagca agccttcgat
     7981 caaagcttct ctgtcctccc gtcctcttag cggcgggttc attttccaaa agccgaaatc
     8041 ttcgggaata tcttcgtcga tcaaaataag atgatggggt gaaacttctg ctgttacgtg
     8101 gattcctgct ttttttgcgt ctctgattac acggacactt tctttagtag aaacatggca
     8161 aacatggtaa tgaacaccag ttgcttcagc tagcaacaga tctctagcaa tttgagaaga
     8221 ctcggttacg cttaagatcc cgggaagacc aagttcttct gccttcttgc cagcgtgcat
     8281 aacgccgcca aataataaag attcatcttc tgtatgggct actagggctt tattgttttt
     8341 tgcagcttct ttcatagcca gatacatcgt cccagctgtt tggacaccta caccgtcatt
     8401 tgtgaaagca aaagcacctt cttcaatcag tgcttcttga tcaactaatt tctcacttcg
     8461 cagattttct gtgattggcg catactgcaa taccttcaca actgcatctt ttctaatgat
     8521 gtcatatact tgtctcagct tttctgccgt atcaggtaca ggatttaaat tcggcatcgc
     8581 gcatacagta gtgaaaccgc cacgagcagc tgagcgagtc ccagcttcga tcgtctcctt
     8641 gtatgtaaat cctggctctc tcaaatgaac atggacgtcc actaaaccag gtgtgatcaa
     8701 ttgtcccttt gcatcaaaca cttcatcaaa ttcggcttct gagaatcctg taccgattgc
     8761 tttgattcgc ccatcttcta tccaaatttc tgctggtgtc gtcatatttt ttcgtgtatt
     8821 gatctgaccg tttttaatga gtgttttcat tttttcatcc tcctataatt acgcttttcc
     8881 atttagcact gcttctaaga ttgccattct gacgaatact ccattgctca tttgctgaac
     8941 gatccgcgac tgtaatcctt ccactaatga atcagctaat tctacatcac ggttaactgg
     9001 tgctgggtgc ataatgattg catgtttttg caacttcttc gcacgctcaa ctgttaaccc
     9061 atgttcctga tggtagcctt cttttgagaa actttctttt ccatcatggc gttcgtgttg
     9121 cacccgaagc atcatcatga catcaacttg atctagtagt tcatccaatg gcatataatg
     9181 tccgtacact tcgaattcat cgtcgtacca ttctctagga cctgagaaga atacttgtgc
     9241 gcctagacgt tttaacatct gcatattgga tttagctacg cgagagtgag tgatatcacc
     9301 tacgatggca actttcagac cttcaaaatg accaaattct tcataaatgg tcatcaaatc
     9361 aagcaagcat tgggttggat gctgtccgct tccgtctcct ccattgataa tcgaacactg
     9421 gattgtttta ctttgtatca gttcgtcata atagttctca tcaccatgac ggataaccgc
     9481 tacgtctacg cctaatgctg acatcgtcag tactgtatcg tatagtgttt cccctttttg
     9541 gacagagctt gtgcttgctt caaattcaat cacttctagc cctagttttt tctccgctac
     9601 atcgaagctt ttatgtgtgc gagtactatt ttcgaaaaag aggtttgtag caaagtactg
     9661 gcgcttttgc ggagtccaat tagcaccttg cttgaattcc tgccctctgc ggatcaagcc
     9721 cataacttct tgatcagtaa gtgcctcaac ggtcaataaa tgctttaagc tgatacgttc
     9781 tgatttgatg atcataatgt ttcctcctta gttttcttcg cttcgtgaag tttctggtaa
     9841 gataagattt aaaatgatgc caagtacagt tgctaatgcc attgccgaca atgtaaatgt
     9901 gcctacttca aagacgagtc cgccaattcc tactactaag ataactgaag caatcaacag
     9961 atttttcttt ttatcaaaat cgattttgtt ctcgattaaa atcttcattc cacttgcggc
    10021 aattactcca aataagataa aactgattcc tgagatgact ggtccaggga tacttaaaat
    10081 caaagcgctt aattttccta cgaatcctaa aatgactgca aatactgcag ctccaccgat
    10141 cacaaacacg ctatggacac gtgtgatagc cagaacacca atgttttcac cgtaacttgt
    10201 tacaggagga cctccgacaa aggctgccac gatttgtgct aatccatcac ctgtcaacgt
    10261 cttatgaaga cccggttctt caaagaaatt acgtttagtt agtttattca ataccataag
    10321 atggccgata tgctctgtca tcgtcacaaa agcgatgggt gccattgttg tgattgctcc
    10381 aagatatagc tttggttcat attgtacaaa gggaatctca aagttaggaa cagcaaacca
    10441 ggcagcgtct acgattggct tagtatccac gattccaaac agcaaggcta cgatgtatcc
    10501 gctaataata cctagtaaaa tcggaatcaa tccgagaaaa cctcttagcc acatgttgaa
    10561 gaaaattgtt aatcctaatg tgatcagcgc tacagcaata tacttgaaat catacgtgcc
    10621 ctgattatac atagcatttg ttgctgcatt tgccgcaagt cccaacccga taaccatgat
    10681 gactggaccg acgacgattg gaggcaagat tttatccagc catgctgaac cgatcttttt
    10741 gatgatcagc gcaacaatca aatagactaa tccagttgtg actgctcctt gtgcaatcgc
    10801 tggataacca tctgttttca tcaaaagctg catcgctgca atgaaagcga agctgcttcc
    10861 taagtacgca ggtatctttc ctttagtagt aaataagtac accaatgtac ctagtcctga
    10921 actgactagc gcaatacctg gatcgatacc tactaagatg gggactaaca cggttgctcc
    10981 gaacatcgtg aacaagtgtt gtaaacttaa tcctacccaa tgtcctgggg ttgggcgatc
    11041 gtggatatct aacaccacgt catcattatg aaatcctttt ttctccgaca tgtttctctc
    11101 tcctctaatc ttcctctttc aaaatcatga tgcgatcttg tccatcaagc tcttgcatct
    11161 ctactaaaat ctcttccgcc cgagatgttg gaatgttctt cccaacataa tctgcacgga
    11221 ttggcaattc tcgatgccca cgatcgacca ggactgctaa tgaaattttt cttggtctgc
    11281 caaaatccat tactgcgtcc attgctgctc gaatcgttcg tcctgtatac aaaacatcat
    11341 caatcaaaat aacttctttt ccttctaagg aaactgggat gtcagaagaa tgaagttccg
    11401 gttcttctgg attttctttt ttgtcatctc ggtataaagt gatatccagt tctcctacag
    11461 gaatatcgat atcttctagc tgcttcaggc gttcggcaat tcttgatgca atgtagattc
    11521 ctcgtgtttt gatacctacc aatacgatat cttggatact atggtttctc tcgataatct
    11581 cataagtaat tcgagtcaat gctcgcttca tggttacctg atctactact tcttttgctt
    11641 gcattttcat tccccctact tttttgcata aaaaaactcc tgccctaaga ggacaggagg
    11701 aatgtttaga ctgagcgtat actgatacac tgatagctat actatgtcaa tgagcaatct
    11761 acttatggcg caccatacgt atccttgctc ccacccttct tagcctcacg ggactaaact
    11821 taaaggaatt ctattaagtt ttatgctttc gttgttaatt actataccgt tatctaaatt
    11881 aaaatgcaat gttttttctt aatttttcta aagttttttc aaaaacttct ggaagaggtg
    11941 cttcaaagat catcttttcg tttgtagtcg ggtgcgtaaa acctaacatt tgcgcatgga
    12001 gaaactgacc attgcctttc aaagttttct tcggtccgta tactggatct ccagctaacg
    12061 gataaccgat atacttcata tgcacacgga tctggtgtgt tcttccggtt tctagtttta
    12121 attcaaccag agtaaatcct tcgaaacgtt ccaatacagt aaaatgtgtg acagccgact
    12181 tcccatcttc tcttatcgcc tgcattttcc ggttttcctt cgaacggccg attggcgcat
    12241 tgatggttcc tttttcgtga ggaatctctc catggactaa agcaacatat ttgcgcaaag
    12301 aggtcttatc ttttaattgc tgagctaatg attcgtgtgc ctggtcattt ttggcaacca
    12361 ttagcagtcc ggaagtatct ttgtcgattc ggtgaacaat acctggacga ataacgccgt
    12421 tgatcgtaga aagatccttg atatggtaca tcagcgcatt gaccattgtg ccatgcgtat
    12481 gacctgctga aggatgaacg accattcctt gaggtttgtt tatcaccgct acatcttcat
    12541 cttcgtaaac gatcgtcaac gggatatttt ctgggaccac atctaatgtt tctggttcgg
    12601 ggatcaagac agtaatctcg tctccttgtt ttactttgta atttgctttg actggctgtc
    12661 cattgacaga aaccgctcca tttttcagcc acagctgaat ttgcgaacga ctgtaatcgg
    12721 tgaataaatt agtcaatact ttatcgatcc taccagattc atggtgaatc gtcgttttaa
    12781 gttctgtcat ttttttcttt gatccttttc atctaaaata atataaatta acatacaaat
    12841 cactccacat acaagtgtca catctgctac gttaaaaatc ggaaagctca taaaatctgt
    12901 ctggaacata tctactacat agccaagacg aatacggtcg ataaaattgc caatcgcacc
    12961 agctagaatc aaactcaatc caatcatgaa ccagcgatta cctttttcac gatttttaat
    13021 catcaatgtg agaatcacaa cgactgcaat gagtgtcact atagtaaaga accacatctt
    13081 cccttctagc atactccatg cggctccagt gtttcgaata tgattcagtg acaaaatacc
    13141 tggtataaga tttttcgtct ctccaagcgc tagattatca gtgatccacc atttgaccaa
    13201 ctgatccact cctactagca aagcgctgaa tatccaataa ataattaaca aaaaatcaat
    13261 ccttttctat ttgtttataa atatcacgag gtcgtacgat cccgagcaga ttaccatcgg
    13321 gattcccatc tttagtgata agtacggctt ccaaagtccc ttgctcacga aacatcgctt
    13381 caacctcgta aatatgtgtt ttagctggaa caaaatgata atttgtgtac tttccgtttt
    13441 ggatgatcaa atccaacgct cgtttatttg ttaaatcaac cggacctttt tggctttctt
    13501 ttgctagcca aaacccgata ttacgtaaag taatcaatgc tacaaatttt cctttattgt
    13561 ataacggaaa ttgtgaatac cttttttctg ctactatctt taacagttca agtaacggaa
    13621 gatcttgttc aaaacctgtt acacgttttg caaatcgagg cagcacggtt tcaggctgaa
    13681 tcagttcttg ttcgatcagc tggattcgct tgactgtcca atcatttggc tctgcaatga
    13741 caaaatcgac tgcgatccga tcatggacga tcgcattcct taactgagca agctgtaaaa
    13801 gatcatcttc gtattttctg atcattaatt gttttctttg agctaggcgt ctgacaagtt
    13861 cggtaaatcc catatttcgt gcatttccca tctcgtctct catccatttt tcgattcgat
    13921 tgaaactgat taaaaaaaga tcagagtttg ccataatttt cctccttgat caaatatgcc
    13981 cggtttttta tatagaaaaa gaacgcaatc aacacaataa ctgcaaaaaa actgaaaaat
    14041 aaattttgta ggaaaacggc ccccaaccca gttgctagaa ataaaaacgg atagatttga
    14101 agaatcaagt aattcattgt gatcacataa gcaggctttg cttctttcac tgtataaacg
    14161 gggcttttta gccaataaaa ggtgacctgc atcgtaaagg gcacttccag atcaagcgtt
    14221 atcttttgtt catgactgag aacactgcgt ttttcattgt tgattcttag ctgaatcgag
    14281 ctacccatac cgtaaaaacc ggtttttctt ataaatgtcg ttttcatcat accctcctat
    14341 tatgctttag tatagctcaa aatacaataa agaaaaagaa tgatcctagt gtaaacaaaa
    14401 ttaattccct atcgtctaaa aaaagaagct gtagtcaaaa tacagctcct tcgatggtag
    14461 attataatca agcttaaaac aatcccaaca cttctccgtc atttgtcacg tccatattca
    14521 atgcagcagg ttttttaggc aaaccaggca tcgtcataac atctccagtc aaggctacta
    14581 aaaatcctgc ccctaatttt gggatgattt cgcggatcgt gatggtgaaa tcttccggtg
    14641 cacctagcag tgaaggatca tcagaaaatg aatactgtgt ttttgccata caaatcggca
    14701 gtttgtccca tccattcttc gtaaactctt ctatttgttt ttgcgctttt ttgctgaaaa
    14761 caacaccatt tcctccgtaa attttcttga cgattgtttc tgtcttttct tgtatcgttt
    14821 cttctaggcg gtataatggc ttgtaattag cttcttttct atcaatcaat cgtactacag
    14881 cttttgctaa atcaacgccg ccttctgctc catctgccca tacactcgct ctcttggcaa
    14941 gaatcccctg attttcacac aagtgctcca acagtgtgag ttcattgtct gtatctgtta
    15001 cgaattcatt gattgcgacg atcacaggta gctgatattg ttccatatta cgtatatggc
    15061 gctttaaatt tgcaaaacct actttcaaag catcaagatt ctcattttta agttcatctt
    15121 ttttcattcc gccatgcatt ttcaatgcac ggacagtggc gacaatcacc acagcatccg
    15181 gcgctttttt caagttaggt actttgatat ctaaaaattt ctctccgcca agatctgcac
    15241 caaatcctgc ctctgtgaca acgtaatctg ctaatctcaa tgctgtcttg gtagctaaaa
    15301 tactattgca gccatgagcg atattggcaa acggtccccc gtggacaaaa gctggtgttc
    15361 cgtaaattgt ttgtacaaga ttcggcttga ttgcatcttt cagcagtaat gccagagcgc
    15421 cttctgcttt caagtctcca gcagtcacag gctgacggtc aaatgtatag ccgacaacga
    15481 tccttgcaag acgagccttt aaatcttcaa ggtctgccgc aagacacaag atagccatga
    15541 tttcgctggc aactgtgata tcaaacccat cttctcttgg aacaccttgg attggcccac
    15601 ccaagccaac aattacttta cgcagttcac gatcattgag atcgactacc cgtttccaaa
    15661 tcactcggcg ggcatcgatg ttcaattcat ttccttgatg aatatgatta tctaaaagag
    15721 cagataatgc attattagct gttgtgatag catgcatgtc accagtaaaa tgcaagttga
    15781 tatcttccat aggcaatacc tgtgcatatc ctccgcctgc agcaccacct ttgattccca
    15841 tcacaggacc aagtgagggt tctcgcaatg caataacaga ttttttattg attttattga
    15901 gcgcgtctcc taaccctaca gtgatcgttg actttccttc gccagctggt gttggattga
    15961 tggaagtaac taagatcaaa tgcccctcag cgttatcctt tgatcgattg attccagaaa
    16021 aattgatttt cgccttatat ttcccataca gttccaaatc gtcttcctta agatctaatt
    16081 tactagccac ttcaacaatc ggttctagct ctgtttcttg tgaaatttgc agatcattct
    16141 tcatttgatt accccttttc acgaacttta ttcaacaaaa tatcttcttt gttaagatat
    16201 gccaattata ccttttttta ttcagaaaaa aaacataaaa ttacaggttt ttaatttatt
    16261 tgacgtactt ataaatactc tttataatga aggctatgat cagactttag aaaggtcgtg
    16321 agtgctttga ccacaggaat tgtaaaatgg tttgacaaca aaaaaggata cggctttatc
    16381 agttatgatg acacagaaga aatttttgta cattttactg cgattgaaga agagggattc
    16441 aaaactttgg aagaaaatca agtggtcgaa tttgagatca tcgaagggaa tcgaggaaca
    16501 caagctgccc atgtcaagaa aggcaacgtt cagactaccg atactagtat gcatgaatag
    16561 ataaacaaat agagtgagac gctaaacggg atgaaccgta cgtctcactc ttttcttttc
    16621 ctgtaaaaat tcgttttttt aggttctgat ttttaaaatt agctttttct ttttttcgta
    16681 taaggataag gaattgattc tgatttgggt ccgttttttt caaaatgatc aaaaaggact
    16741 acttgatctt tttttcgcat catgaaaaca gatggggctt tctcatcttg caacatcatt
    16801 tgcaccccag tattagttgg cgtcaaagat cgaatctgtc cgacagatat ttctcggtct
    16861 ttccaaaaag cagcataacg tataatcaca gagtttgatt ttaaaataaa agaacgccgc
    16921 aaacctagaa atagaaaaac aagaaaaata cctaaaacaa aattgctttt ccaataagga
    16981 cctgtgtttt ctaaagaaag aatcagacta aaaaacagaa tgatcaaggt caatgaccaa
    17041 tagatgatcg tatgtgccag ttcaggctgc catcgaatat tttttttcat tctctaacct
    17101 ctcaccgatt cagtaatgat ataatcttac cataacatta gtttgaacaa aaaggagaat
    17161 ctctttttat gttaaaagta tacattgatg cctctacaaa aggcaaccct ggtcctagtg
    17221 gaggtggaat cttgattgtc tatcaaggta aacaagaaca gctatctatc ccgctttcta
    17281 tagggacaaa ccatcaagca gagttcgaag tctttttaaa aactttagaa tttctaaaga
    17341 aaaacgactt acagaatgaa acgattttct gtttttcaga cagcaaaaca ctagtttcta
    17401 cggtcgacaa aaacgcaacg aagaacgagc tcttccttcc ctaccttcaa agaatccaag
    17461 aacttctatc cgaattttct ttattgatcc tgcaatggtt gcccgaaaac aaaaataaag
    17521 gagcagataa tcttgcacgt caggctctcc ataaacaatt aaaagaaaaa aagactcttt
    17581 aaattaaaaa ctttaaggag tctttttata gtttctcatt taatataaaa gataattgtg
    17641 atcaaacgta atttccaatt ggtaatctcc gattttttct tctatttgac tacgtaagat
    17701 tctttctagt tctgtatcaa caaactggaa tttctcaggg accactacat caaattgcag
    17761 cgtttttcct tgatgaatta ctctaaaatc atgaatatta agccctgtac ctgtttcaag
    17821 aagaatttgc tttaactgcc gcaagatatt atagtatttt tcgttatcaa caggcattgg
    17881 atccaagtga cagacaagtt ccacatccaa agacttttta aagtcttttt ctatcagatc
    17941 aataatctta tgagccacat tcaaattcat tgattcatct acttcgatgt ggactgatgc
    18001 aaagcgttta ttgggtccat agttatgcac caataaatca tgatagccaa gtatatcatc
    18061 gcaagcattt aaatgctctt ccatttcttc aatctctgct ggcgttgggc gacttcctaa
    18121 tagttcgtat acaaaatttc gaagcatcgt caatccacta taaaggatat ataatgctaa
    18181 agcaaaacct atatatccat caatacgcca tccagttacc cattcgattg ctgctgaaag
    18241 caatacagcc agtgtagtaa agacatcatt ataactatct gttgctgtag ctctcaatgt
    18301 ttcagaatca atggattgcg caataacctg atacattttg ccttgtgcaa atttcaaaaa
    18361 gatggaaatg attaggacaa aaaagacgat gggtgttaaa gagacatttt ctggatggat
    18421 gattttttca gcagaagact ctaaaaattg aaatcctaca taagtaatga tgatcgaaac
    18481 aaataaccca ctgatatatt cgaagcgttc gtgtccataa ggatgttcct gatctgctgg
    18541 ctttgcagca attttaaatc cgatcaaagt caatatggat gaagcagtat ctgatagact
    18601 gttcactgca tcagccataa tggagacact tcctgaaaaa agcccgatca ttagcttaga
    18661 gacaaacaag atcagattcg taaccaatcc taagataccc gctagtactc ctttttttgt
    18721 ccgctgttgc ggtatttgtt tctttttttc ttcttgtttc ttagccaagt tttttacccc
    18781 ttctagtttg cttgttgctc aggtaaaaag atggagagga cgattgtttt cctctcctct
    18841 cttccttttt ctatctatcg tgcgtcctat tactattaaa agatggtcat tattctggtt
    18901 tcaacggtgt tgctgcaaat aacgcttttg ctagtaatgg tgttatgatt cccgcaccta
    18961 ttgcttctgg tatcccattg attgctgcaa cccctgctaa ggttttaaat aaaagagcag
    19021 agtctacacc ataggcatct gccgttgcac ctgtataaaa taatcccatg aatcctaaca
    19081 ctaagactgt attgaccaat gaacccagta cccctgcaaa ggctgagctg atcgttattg
    19141 atttattttt ttgataaatc cagtggaaaa ccaagcctgc tactaagcca acaagtactc
    19201 tgggaactac cgagataatc ggattcgtga acactaatgt atcaaatggc gttgttggca
    19261 tggcataggc acgaacaatt gtagcgattc cccaaaacaa gccaatcacc atgccatctt
    19321 ttgggcctaa tactactgca gcaataatta ctgtgatatg gataattgtt aaactaatga
    19381 atcctaatgg aataaacccc agccaaggaa ccatcgcttg tacgatgatg attgctaaca
    19441 agatcgcacg taagactagt cgaaaggtct tatttctact attcattttt tcaccccttt
    19501 ttatttatac taatcgtaaa aatttctgtc actttcgtca actaaagacc atccgaatcc
    19561 gaccatttga ttagatgcgc catccaattg aaaaaagtat gagggcaaga cttgtttgtc
    19621 ttcgccccca taatcatgtt tcgttcaatg tttgaacttt tgtttttctg tcaaacgtaa
    19681 acgccctatt gttgcgcaac ccattctttc gcagctttaa gtgcatctgg aataccagca
    19741 ggatttttcc cgccagcttg tgccatgtct ggacgtccac caccgccacc gcctactttc
    19801 ggtgcgattg ctttaatcaa gtcgccagct tttagtccag cttcatttga ttctttcgtc
    19861 attgccgcta gcagactgac ttttccttct tgttcgtttg ctaaaacaag aatatctgaa
    19921 atattttttt gtttccattg gtcagctaat tgacgcagct gattcatatc tttcactttt
    19981 acttgtgctg caatgattgt ttttccattg atcgtttctg gatctttgaa tacatcacca
    20041 gcttgttgat tcgctaactt gctagctaat tgttcatttt ccttttgtag ctctcttact
    20101 tgctgttgca attgttccgc ttttgaaaca acttctttca attgaggtga tttaacaatt
    20161 gttgctactt gttttagttg tttttcttct tcttcaagta gttcgtacgc ttctttactt
    20221 gttacagcct cgattcgtcg cacgcctgca ccaattcctg attcagaaac gattttgaag
    20281 attccgatgt tttctgtatt tgctacatgt gtgcctccgc acagttcgat tgaccagccg
    20341 ccaatattta ctactcgaac gtcatgtccg tatttttcac caaacaaagc cattgcaccc
    20401 atgtttttgg ctgtatcgat atctgtttca attgtttcaa ctgggatagc ttcccagatt
    20461 ttttcgttga cgattctttc catttcagct aattcatctg aagtgatttg accaaagtgg
    20521 gtaaagtcga atcgtaaatg tccaggagcg actaaagaac cagcttgatt tgcatgctcg
    20581 ccaagtactt ctttcaatgc cttgtgcagt agatgggttg ctgtatggtt tttgatgatc
    20641 cgattgcgca tcgattgatc gatcaataga tgataggttt gtccttcact cattggtgct
    20701 tttacttcta cggtgtgcat aaattgacca ttaggcgctt tttgaacatc tgtaacaact
    20761 gctacgattt cgcctacttc attcatgatc catccttgat ccgctacttg cccacccatt
    20821 tctgcataga aaggtgtttg atcaaagatc atttgagctt caccttctgc gacttcattc
    20881 aaaagagact cacctttaac gatgaccaat aattgactat ctgattcttg ttctgtgtat
    20941 ccaatgaatt tactttctac tttgatatct gtcaataaag cagattgtac ccccattgat
    21001 ttttcagtac tgcgggcaga gcgagcacgt tcgcgttgtg catccatttc tttttcaaaa
    21061 ccagcatgat caactttcag tccttcatct tctgccacct cttccgttaa ttctaccggg
    21121 aagccatatg tgtcgtatag tttgaagatg tcttttcctg ctagagtcgt cccgttttcg
    21181 ttttttactt cttggatcac ttgatttaag atttccaaac cttcattgat tgtttcattg
    21241 aaacgttctt cttctgtacg gataactttt tcaataaatt ctttttgttg aagtacttca
    21301 ggataataac ttaccatgat ttccccaaca acagggacta aacgatataa gaatgcttgt
    21361 tcaataccta attttttccc atgcatgaca gcacgacgaa gcaaacggcg taagacatac
    21421 cctctccctt cattcgatgg caatgcaccg tcgccaatag caaatgaaag ggcgcgaata
    21481 tgatctgcga ttactttgta agaaatatct gttactgggt tttcaccata ttttacttgt
    21541 ttgcttagct tttctacggc gtggataata ggcataaaca agtctgtctc aaagttcgtt
    21601 gacgcatctt gaacgattga gaccatgcgt tccaatccca tccccgtatc aatgttttta
    21661 tgaggaagtg gttcaaatgt gttatcaggc ttatggttga attctgaaaa tactaagttc
    21721 cagatttcta aataacgttc attttctcct cctggatagt tttcaggatc gtcttctgct
    21781 aaatcgttat aagcttctcc acggtcatag aaaatttccg tatccggacc acatgggcct
    21841 gcaccgatat cccagaagtt gtcttctaca tcaataatgt gatctagtgg caggccaact
    21901 tcttcatgcc agatgcgttt tgcttctgta tcttttggat aaactgttac ataaagtttt
    21961 tctggatcga atgccatcca ttttggatca gtcaaaaatt cccatgccca gtggatggct
    22021 tcatttttga aatagtcacc aatcgaaaaa ttacccagca tctcaaacat cgtatggtgc
    22081 cgtgctgttt taccgacatt ttcgatatca tttgttcgaa tagatttttg ggcatttgtg
    22141 attcttggat tttctggcac aactgaacca tcaaaatatt ttttcaatgt tgcaacacca
    22201 gagttgatcc acaataaggt tggatcgttc acggggatca aagacgcact aggttcaatc
    22261 gtatgtcctt tagattggaa gaaatcaaga aacatttgtc gaacttgtgc actacttaat
    22321 tgtttcatgt cattacctgc tttctataaa ttaaatacaa aaaaacacca tcacagccaa
    22381 ggacgaaaac tcgcggtacc accttgattg caatgatgtg taatcattac ctcttaaact
    22441 cgataacgct gagctgcgtc ggtatttcta ccaaaataga aaaagggagc aatttgatta
    22501 gtgactattc ttttttcagc caagaaagaa ttttctgtga gtcttaaaaa acaaatcata
    22561 tccttttcgt acttaattag tatacgcatt ctattgatca ctgtcaatat ctcaattttt
    22621 attttcattt ccttttggtc atactccacg atttttggta cgataagaat aaaggaatca
    22681 atacatctct gctattcatg acaaggagga atatgatgaa aaaaatagtt ggagcggatg
    22741 aatggatacg cgcagctagc tgctttttga gatatcaagt ttttgtatta gaacaaggga
    22801 ttcctgcaga actagaattc gacggaaaag atatcccgca acaaacctat tatctatttc
    22861 ttgatcaaaa aagaccagta gctactttgc gttatgaaaa gaaaacacct acttgcttca
    22921 agccggaacg attttgcgta gcaaaagaat accgaggaaa agggttaggc ggtcagttgc
    22981 ttgaagctgc tgaaaaacgg gcggtttcag aaggttgtca gactagttat gtagtagctg
    23041 aaatgacggc tgtagatttt tatgaaaaac aagggtatca tacttgcaca gaacctttta
    23101 ttgaagatga agtactttgt atcggaatga aaaaaagtct agcttaaaaa caagagattg
    23161 tgacacgcct tttgtcacaa cctcttgttt tttatttatg tgaatttttc ttatttttac
    23221 gagcagtacg gctttgctgt ctgcgttcga ttttgcgttc ttttttattt ttttctgcaa
    23281 ttgcccaatc gatttttttc ttataaccag gcttgatctt tttctttttc ttcttcacaa
    23341 gaccaatcag cgtgggatct aatacatctt ttgatttttc tcgtttggca cgtctattac
    23401 gatcgtatgt tttgacgatt tccccgtttt tgatttcttt tggttcaaat gagacgccca
    23461 atttttctat cgcatcaatg gcttgctcat catttggcga atataacgta atggccgttc
    23521 cttttaagcc attacgtcca gtacgtccga cacgatggat aaagaagtct agatcttctg
    23581 gaatttctgc attaataaca tgagagacac cttcgatatc gatccctcta gcagctaaat
    23641 cagtcgctac aacatattga tactccagat tctgtacttg tcgcatcact cgttttctct
    23701 ctcttggggt gatgtctcca tgaatctttg ctactttcaa gccttggttt ttcaagtaat
    23761 cggcaatttc atccacatgc tgttttgtat tggcaaatac gattgctaaa taaggatgac
    23821 caattgtcag cagttgataa atcatttgat tctcatcttt accttttgtt gaaatcagcc
    23881 aattttcaat gtcttcagaa ataactgctt ttggtttgat ttcttcaatg attggatttt
    23941 ccatgtattt tcgtaagaat ggacgaagtt tttctggaat agtggcagaa aaaactaaaa
    24001 actgcagctt ttctggtaat cgacttgcaa tttggtctac ttcatttaaa aaacccatat
    24061 ccaatgtcat atccgcttca tcgacaacaa atgcaaatgc ggtgtgaatc ttcagcgctt
    24121 gttcattcat catgtctaat attctaccag gcgtgcctac tacaacctgt ggctgttggt
    24181 gatgcagacg atttaattga cgctgtttgt ctgtaccgcc gacaaaattc gagacacgga
    24241 tctccggtgt agaaaattta gcgatttgtg tagctgcttc atagatctgt gcagctagtt
    24301 ccctacttgg agctgtaata acaacttgta cttcctcttt ttctgcattg attttttcaa
    24361 acaaaggcaa caaaaacgta tgtgtttttc ctgtaccagt ttgagattgc ccaatgatat
    24421 tttttccttt tgcaattaaa ggaatcaaac gttcctggac ctctgtgggc tgtttgaagc
    24481 cctttgcggt taacgcttca ttgataaacg gtttgaagcg gaatatatca aaattattca
    24541 ttcgtgcacc tcttctcttt aaattatcac gactttggca ttataacaca gacaaagcgt
    24601 ttttgctcag ctaatttcga gaatcatcat cctttgtttg gccaatcgtg ctaacagtat
    24661 atgtgtgact agctttttca taaaacagaa tccgtttttt tctcaaaaat gatagcaaga
    24721 tatttaaaat aaaaatacct tgtagtaaca agacaccgag agaaatgaat actgcactca
    24781 tctgcaacac cgtattcgtt gtcgaattca tgatttgctg aagcaactgc cccaaccagc
    24841 ttaaaccata aaaaagaaag tacagatacc cgtagcggat catcaatgct tttaaagaaa
    24901 ttcgttcgcg gtcttcttcc accaaacgga tacgcacaat ttttttgcca agtgtccatc
    24961 cattggttat aaacggtaca atcataaagt atacccctac ttctaataaa agccaccaga
    25021 tatgagaagt aactgcccct gaatcgaacg caattttaaa gaaaatcgcc gctaccaaat
    25081 taataagaga taaaaatgtc cagtcgatca aataagcgac caagcgtcga gtcaatgaga
    25141 cagtctttcc tttttcataa gaaagttcat ccattcgtct tcttgtagga agcataaatg
    25201 taaataacgg cgtaacgaca aaaccaatga ttccacccaa tgtatttgtc actaaatcat
    25261 tcacatcaaa taagcggtat ggtcttgggt agataaaata aagtccactt agctgcgtca
    25321 attcaaaaaa tagagaaagg cagaaactag caatcatcgt tttcataaaa gaaagccgga
    25381 aataatatct taaataaatg ccaaatggta tgagtaataa aacattaaaa gctggttcca
    25441 aaaatacact ctgcttcatt gccggcaggt aggtactagg ttcagacaag accaataccg
    25501 tgttgtctct gaaatgttgc catgaagcaa ataaatgcag ctccatcttt ggccctgtca
    25561 tttgggcaac cgtctccttt gccggaagtg gtaagatcac caaaaaatac gcacacaata
    25621 aataaaaaac gaaagaatac aaaataactg cccttgttac tagaaatgtt ccatactttc
    25681 gatattcata aataaaaaaa acagatgaga tcgctaaggc taaaaatgga aaaacaatca
    25741 gcgcaaaacg aatcggttcg atataacttg ccatatgact cctccttgat tctttgtatc
    25801 tctcttatct tagcagtcct ttgctgtttc aagcaagtaa cgaggctatt atttctcatt
    25861 tctttcaaag taaagtaaga taaacacaaa gggggtgtcg agatgctgaa tatgtacttt
    25921 gtttttggcg tacctatttt tttactgttc ctttatgcta cgatcgctta tgtaagaaaa
    25981 agaactacaa ttcattattt aggatttatt ctcctgatta tttcgggttt catgctagtc
    26041 ttcaatcttc agacttggca gcaagcactt cttgaaatgg ataaaatgac tccacatgct
    26101 ttgtctaaag tgcttggcta ccctgtctat ctgatttggc ttcccatttt catttctggt
    26161 tgcttggttt tattaaatat ttatcgcggc gtacgccgaa tagttcagtt acgcaaatca
    26221 aagtaaaaga cagaaatcgg ttgagcccga tttctgtctt ttactttgct atttcatcgt
    26281 agacaaaacg acatttacgt gatcaaaatt aacgagtact tttgacattt cttccacgta
    26341 ctcctcattt aaataaaata ttttgtttgg cagagaagtg cagttctaat aaaattcgaa
    26401 tgttttagct atctacgttt taaatagaga taaatcggga taccagcaag tgtcaatact
    26461 atccccatcg aagctaaaaa ggtctgagtg ataagcgtgc ttgatacgat aaaagtccct
    26521 ccaatgattg ctactagtgg aataaacgga tacattggaa ctttgtatgg tctaaacaaa
    26581 tcaggttctt ttttacgaag aagaatgaca ccaacaaaga ccatcgtata aaaaatccaa
    26641 atgacaaaga tcagcatatc cgttagtgta tcaaaaccac cgatcatcat catgccaata
    26701 gcgatgacta gctctaagat acctgcggct acaggaactt tagttttatc atgaagttta
    26761 gcaaagagct tgctaaaagg caggttgttt tcttttgcca taacataagg gagacgcatt
    26821 ccagtaaggg tatagccatt gatggttcca taaacagaaa tcaagatacc aactgtgacc
    26881 agtcttccac cgaagccgcc aaaaatcatt tttgcaacgt cacttgccgc attgctgttt
    26941 cctgcgactc cgtcgataga tgcggttctt aagaacaccg catttactaa taaataaacg
    27001 atcatgattc cgatgattcc taaagaaatt gcttttggca aatctttcgc cggctttttc
    27061 aattctcccg aaatatttcc tacgtgtatc caaccgtcgt atgcgaacat cgttgctagc
    27121 aagcccgctc ccaaggctga gaaaaatgaa agattttctc ctgcttggat cggaaacagt
    27181 tggaagtcta ctccttcttg tcggaacaaa ccgaaaatga cgatcacaaa caaaggaata
    27241 agcttgcaga caagcgtaat cgactgaaaa gcccctccag ctttagaacc taaaaaattg
    27301 atcaacagga tcgatacagc agctgtaaca gctacgggaa caatcattga ctgggaaaga
    27361 ccaaatagat tgacgaactg tgtaccgaaa atgatcgaca aggctgctac atttgctgga
    27421 aaataaatca cgacttgcgc ccagcccaat aaaaaagctg ctgtatttcc gtaaatccgt
    27481 tcgatatatt taatcatccc tcctgtttct ggaatagcag ctgccagttc agctcctgtt
    27541 agtcctgcac agacagaaat cataccaccg agaaaccaag aaaacatatg caaactggcg
    27601 gatcctgtaa cttctgctac gcttgcggct ttaaaaaata cccctgcacc gatcactgtg
    27661 cccataacag tcgataaagc agtaaaaaat cccatcgtac gttttaattc attttctatt
    27721 tttacttctt ttactggcgt ggcttgatat tcttccatcc cttgtcccct ttcataccat
    27781 agccaatccc aaaagtataa aaaacttttt ggatgtggtt cttgttttat accgtctcta
    27841 ccaatttttc tttcagtttt tccaacatat cctttgtcat cacatctaag tcgtatttcg
    27901 gcgaaaagcc ccattcctct tgcgcacaag aaacatctag tgaatttggc caagattctg
    27961 ctattgactg acgtacagga tccacatcat aatccatttc aaaatctggt atgatttttt
    28021 gaatagatgc tttgatctct tccgggtcaa aagacatcgc cgatatattg aaggcatttc
    28081 gatgaaccaa tcgatcagga tctgcattca tgagttttat aatggcatcg atcgcatctg
    28141 gcatatacat catatccatt gatgtacctt gatcaatgaa actagtgtac tttccttctt
    28201 tgattgctga ataataaata tcgactgcgt agtctgtcgt accgcctcct ggcaaggttg
    28261 cataagaaat caatccaggg aaacggacac cacgagtatc tacgccatat ttcacataat
    28321 aataatcaca tagtagttct ccagctacct tcgttacccc atacatggtt gtaggacgct
    28381 gaagagtatc ttgtggggta tgatcttttg gagtatttgg accaaacgcg ccgattgaac
    28441 ttggcgtaaa aaatttcagc tggaattctc tagcgacttc caatgcattg accagcccag
    28501 tcacgttcaa attccaggct aattgtggtt ttgcttctgc aacagctgac aaaagagcag
    28561 ccaaatgaat caatgtgtcc acttgatatt gttctgctat gctgcgcatt ttttcgtaat
    28621 ccattacatc taggatctca aagatatcgt ttttctcaat gacttcaggc atgcgtatat
    28681 ctgtcgcaat gacattttca ctgcctagtt cttgacgtag ccgtgtgatc aactctgaac
    28741 caatttgtcc taaacatcct gtcaccaata cttttttcat gatgatcctc cctgatcttt
    28801 tttactacta tcttcagatg attaacaata tatgcgtgaa catgttctct aattttaaat
    28861 agacgaaaaa actaaattta gttaaataat ttccatttct tttcctattt tttgataaac
    28921 tgctactgct tcatcaagca tttccctcgt atgttccgca gtcggcatat ttctgatcct
    28981 gcctgtccca agaggcacag ttggataaac gattggttta gcataaatcc catgttcgat
    29041 cagcttctta gagaaagtct gagtcacttt ttcgtctccc aaaatgactg gagtaatagg
    29101 tgtttcagac ataccgatgt tatagcctac ttttttcaat tcttctttaa aataattagc
    29161 attttcccaa actttttcga caagttccgg atgttcttgc atcaaagtaa tcgaagccaa
    29221 tgcagctgca gctgcacctg gagttagcga agtagaaaat aggaatggcc gacttctagc
    29281 tttcaaccag tcgattagtg ttttcgatcc agctacataa cccccaacaa cacctatagc
    29341 tttcgataac gtgcccattt gcatatcgat tttgtcgctt aatccaaaat gcttgacggt
    29401 tcctgcacct tttccagtta cgcctgagcc gtgcgcatca tcgacgtagg tgatcagtcc
    29461 atatttttca acaatcggaa taatttccgg caatcgtgca atgtctccat ccatggagaa
    29521 aacaccatct gtgatataca tgattttttt atatttctta ctttctatcg cttcctttgc
    29581 ttttttctca aggtctttca tatcttgatg tttaattcgg atgatttttg ctccggataa
    29641 gcggcaaccg tcaataatag atgcatggtt taattcatcc gataagatag catcttcttt
    29701 tgtcattact gcagagatag cgcccatatt gcagttgaat ccagattgaa aggcgattgc
    29761 tgcttctgtc cctttgaatt ctgcgatttt ttcttctaat tgctgatgga ttttcagcga
    29821 accattgatc gttcggactg ctcccgctcc tacaccataa gtttcggttg cttcaataca
    29881 tgcttttttc aattcttcgc gattggcaaa tccaagataa ttgttggatg ctagattgat
    29941 catctttttt ccgtctacga tgatacaagc accattttca ctttctagta tgtcgatcgt
    30001 attgtataac cccttttctt tcagttgatc caattctttc tctaaataca ttgttagttc
    30061 tttggaagac attgtctcca cctccatatt catctgtctt gcagaacata ccaaacatat
    30121 atcgtgtcat tcatctacaa agcaagtatg ctatattttt gataacgctt acactgatta
    30181 tgagaattat ttaaccacat tttttattta ca
//
LOCUS       DACDEG010000023        29592 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.023, whole genome shotgun sequence.
ACCESSION   DACDEG010000023 DACDEG010000000
VERSION     DACDEG010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.023"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..376)
                     /locus_tag="IV142_001135"
     CDS             complement(<1..376)
                     /locus_tag="IV142_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HAP8484806.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFP"
     gene            complement(488..1519)
                     /locus_tag="IV142_001136"
                     /pseudo
     CDS             complement(488..1519)
                     /locus_tag="IV142_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(1601..2290)
                     /locus_tag="IV142_001137"
     CDS             complement(1601..2290)
                     /locus_tag="IV142_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8484807.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(2396..2764)
                     /gene="rplL"
                     /locus_tag="IV142_001138"
     CDS             complement(2396..2764)
                     /gene="rplL"
                     /locus_tag="IV142_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP8484808.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2817..3320)
                     /locus_tag="IV142_001139"
     CDS             complement(2817..3320)
                     /locus_tag="IV142_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP8484809.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3730..4419)
                     /gene="rplA"
                     /locus_tag="IV142_001140"
     CDS             complement(3730..4419)
                     /gene="rplA"
                     /locus_tag="IV142_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP8484810.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4523..4945)
                     /gene="rplK"
                     /locus_tag="IV142_001141"
     CDS             complement(4523..4945)
                     /gene="rplK"
                     /locus_tag="IV142_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP8484811.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            5288..5956
                     /gene="sdaAB"
                     /locus_tag="IV142_001142"
     CDS             5288..5956
                     /gene="sdaAB"
                     /locus_tag="IV142_001142"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP8484812.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5969..6841
                     /gene="sdaAA"
                     /locus_tag="IV142_001143"
     CDS             5969..6841
                     /gene="sdaAA"
                     /locus_tag="IV142_001143"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP8484813.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6932..7951)
                     /locus_tag="IV142_001144"
     CDS             complement(6932..7951)
                     /locus_tag="IV142_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP8484814.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(8124..9011)
                     /locus_tag="IV142_001145"
     CDS             complement(8124..9011)
                     /locus_tag="IV142_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8484815.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(9088..9633)
                     /gene="nusG"
                     /locus_tag="IV142_001146"
     CDS             complement(9088..9633)
                     /gene="nusG"
                     /locus_tag="IV142_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP8484816.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9734..9904)
                     /gene="secE"
                     /locus_tag="IV142_001147"
     CDS             complement(9734..9904)
                     /gene="secE"
                     /locus_tag="IV142_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP8484817.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9928..10080)
                     /gene="rpmG"
                     /locus_tag="IV142_001148"
     CDS             complement(9928..10080)
                     /gene="rpmG"
                     /locus_tag="IV142_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8484818.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            10242..10886
                     /locus_tag="IV142_001149"
     CDS             10242..10886
                     /locus_tag="IV142_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8484819.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10942..11445)
                     /locus_tag="IV142_001150"
     CDS             complement(10942..11445)
                     /locus_tag="IV142_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP8484820.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(11458..11504)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12042..13007
                     /locus_tag="IV142_001151"
     CDS             12042..13007
                     /locus_tag="IV142_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP8484821.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(13106..14278)
                     /locus_tag="IV142_001152"
     CDS             complement(13106..14278)
                     /locus_tag="IV142_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8484822.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(14265..15410)
                     /locus_tag="IV142_001153"
     CDS             complement(14265..15410)
                     /locus_tag="IV142_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8484823.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(15424..16356)
                     /locus_tag="IV142_001154"
     CDS             complement(15424..16356)
                     /locus_tag="IV142_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8484824.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            16473..17552
                     /locus_tag="IV142_001155"
     CDS             16473..17552
                     /locus_tag="IV142_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8484825.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17549..18178
                     /locus_tag="IV142_001156"
     CDS             17549..18178
                     /locus_tag="IV142_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8484826.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(18288..18515)
                     /locus_tag="IV142_001157"
     CDS             complement(18288..18515)
                     /locus_tag="IV142_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8484827.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18618..21206)
                     /locus_tag="IV142_001158"
     CDS             complement(18618..21206)
                     /locus_tag="IV142_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP8484828.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(21226..21885)
                     /locus_tag="IV142_001159"
     CDS             complement(21226..21885)
                     /locus_tag="IV142_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8484829.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21934..22437)
                     /gene="trmL"
                     /locus_tag="IV142_001160"
     CDS             complement(21934..22437)
                     /gene="trmL"
                     /locus_tag="IV142_001160"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP8484830.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22658..23524)
                     /locus_tag="IV142_001161"
     CDS             complement(22658..23524)
                     /locus_tag="IV142_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8484831.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23633..24274
                     /locus_tag="IV142_001162"
     CDS             23633..24274
                     /locus_tag="IV142_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP8484832.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(24336..25697)
                     /gene="mgtE"
                     /locus_tag="IV142_001163"
     CDS             complement(24336..25697)
                     /gene="mgtE"
                     /locus_tag="IV142_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP8484833.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25715..26614)
                     /locus_tag="IV142_001164"
     CDS             complement(25715..26614)
                     /locus_tag="IV142_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8484834.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26616..27413)
                     /locus_tag="IV142_001165"
     CDS             complement(26616..27413)
                     /locus_tag="IV142_001165"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP8484835.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(27391..28095)
                     /locus_tag="IV142_001166"
     CDS             complement(27391..28095)
                     /locus_tag="IV142_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP8484836.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            28226..28801
                     /locus_tag="IV142_001167"
     CDS             28226..28801
                     /locus_tag="IV142_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP8484837.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28919..>29592
                     /locus_tag="IV142_001168"
     CDS             28919..>29592
                     /locus_tag="IV142_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP8484838.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLS"
BASE COUNT         8710 a         6252 c         5319 g         9311 t
ORIGIN      
        1 taggaaaact atctataatt ccgactagtt cacctttggt gagcttcttg atgaaaaagt
       61 atcgaataat cttgatagtg taagctaagt ttgaacttaa gcgagtgaat cgactcctac
      121 taggaaagtc accattagga aataagacgg aacaaaccgc tctatacgtg gctctttggc
      181 tagtatagcc attaattatg ccccatataa cacaagcaat aatcactgta tcgcgttgct
      241 tcagttgatc agtatttctt cggtttttaa tcttatctgg tacacaattg tggtatatgt
      301 tagaaacagt tctcataatt tccttgaatg ttataaaagt atctgtataa tgttcagtat
      361 attttgatga acgcatatgt agtcctcatt tcttgggagt tttgagacta ctatatgcgt
      421 ttttttgttg atttaccaac atgtttagac attttcctct aggaaaaaat taactagcac
      481 cacgggtaaa tacagtatta tttcggttgc ttgctgcttc acctagccct tgtactcgtg
      541 tttgattggc gacttgacca aattgattcc cgacgtctaa taaaataata gctaaaagca
      601 aaatggccaa atggcttccg ccaaccaata aagcaataaa gcttattgtt tgcataacaa
      661 gtcccatata cacgattttt ctttcagaat aagaatctgc cagtcgtccc acgataggtg
      721 cagcaactgc tccagacaaa ccaaaaatcg ctaatatccc tgcttcgaat gttccccaat
      781 tgtaggcggg actgctgata taaaaaatca acgtagacca aaagatcgaa aatgttccaa
      841 acatgaaaaa tccactgact gccgcttctc ttaatagacg ttgagaagca actaaatgcg
      901 gcaaactttt aagtgaatca aggtatgaaa cagctgtact actttgctgg acagaacgcg
      961 gcattttcca gtaaagcaga agtgtaagaa ttccgactgc acataaagca aataaataga
     1021 tcatccgcca agaaaaaaga ctggcaacaa taccagatac cgtccgagaa agcaaaatcc
     1081 ccacgagtaa accgctaagc aatgtaccca tgaccttccc tcttgctttt ggtcccgcta
     1141 gtaccgcacc gtaaggaatg atgatttgcg gcacgatcga aagtaatcct attaaaaaag
     1201 aagcgcatgc aaataacgta aacgaaggcg caaaaaaagc ggccaacaga gagacggcag
     1261 ataacgctac cattcgaatg atcaattttg gacgatccac cacgtcccct aaaggaacta
     1321 aaaacagtaa ccctaacgca tagcctaatt gggtcaacat cgtcaatatc ccgactgcac
     1381 ttgtgctaac agataaacta gctgctactt ttgttccaat cggctggatg taatacatat
     1441 tggcaacaac aacgccacaa gtgattgata atagtaaaat aagcgaagtt gttaaaggtg
     1501 ttttttcatt actcttcatt gatttccccc atttcctatc ttagtttaca acttatggaa
     1561 aagattggaa atagaaaaat acaactattc gccacactta ttatttgtgc ttttgttttc
     1621 cttgtttttt cttgcgatct ttataaatga agacaactaa ccctactagc acagtaacac
     1681 ttgcaaccaa cgtgactgca gttttgttat atccgtgaaa cagagcttct ccaccacacg
     1741 catacaacac agacgaaggg atgactccaa tcacgatcga caatatgatt tgctgtagag
     1801 gcaatttcat cgcatctgca gcaaaattga ccatagaaga tggaataacc ggtatcatat
     1861 agcctaacat tacacctatt tttggatgtt tcatttgtcg gattgcttgc acccaatgat
     1921 tcgtttcggt tttcttatct aaaaatttta agcgatgcat aaggaaaatt gcagtgatgt
     1981 taccaagagc attacccaaa atgttgatga tcgcacctgt cagtggtcca aagctcaaac
     2041 cagcaatgac tccaactact gaagtaggaa tacctggaac agcacacatc accgctgtca
     2101 atacgatcaa gagtatcgat gcaaaagtcc catgcgaacg aatatgtgcg agtagctctg
     2161 ccttactcgc tctcggattt aaaaataatt ggatgtctgt ctgatattct aaatacaacc
     2221 gatagcccac taccagtatc agaatgattc ccaacgagat caacatcaat cggattattt
     2281 tctttttcat atttcctcct gaaaaaaatc tatattcatt aaataatagt ttaacataaa
     2341 aacaaaaaag caccgaaatc caacatagga tctcagtgct tttaaatagt aaaaattatt
     2401 taacagttac agatgcgcca acttcttcaa gtttagcttt caattcttca gcttcttctt
     2461 tagatacgcc ttctttaact ggtgcaggag cgccatcaac tacagcttta gcttctttca
     2521 agcctaaacc agttgcttca cgaactgctt tgataacttt aactttttgg tctccagctg
     2581 cagttaattc tacagtgaat tctgtttgtt cttcagcagc agcagcgcca ccagcagcag
     2641 ctacagctac aggagcagca gcagatacgc caaattcttc ttcaatagct ttaactaggt
     2701 cgttaagttc taggatagtt gcttctttta actcagcaac gatgttttca atgttcaatg
     2761 ccattataat ttcctccgtt taattgttct tttttattta atgctttatg cggcaattaa
     2821 gctgcatctc catcttcttt gtctgccact gctttgacag cgtaagccac gttgcggact
     2881 ggcgcttgta gtacagatag caacatagat agaagtcctt cgcgatttgg caattttgcc
     2941 aatgctgtga tttcttccaa tgaagagact ttaccttcga tgataccgcc tttgatttct
     3001 aatgcttttg cttctttagc aaattcgtcc atgattttcg ctggtgcgac tacatcttcg
     3061 ttactgaatg caacagctgt aggtccagtg aatacttcgt ccatgccttc aaggccagct
     3121 tttttagctg cacgtgaaag aatagaattt ttgataactt tcatttctac gttagcatca
     3181 cgaagttgtt tacgtaaacg tgttacttca tcaactgtta atccgcggta gtcaacgact
     3241 actactgatg cagctgctgt aaatttttcg cttacttcat caacgattgc tgcttttttt
     3301 gcgataattg cttcactcat ttttgagttt cacctcctga attttgatgg aagagttttt
     3361 tgcactgaaa aggcaagtca cttttatagt tactttgctc aaccagtatt attcatcacc
     3421 tattcacttc gttcatagtg attctactcg ctttgctcga aaggaaactc ctttcgcttt
     3481 gctcaaccag tattgttcat catctcttca cttcgttcat agtgattcac aataaaaaac
     3541 tctatgccac cgcagacata gagggactat tgaatatatt caataaacgt cctcggtagg
     3601 aaattaaggc ttgatgccac ctactgtctt cggtacaagt taattattaa cctcaactag
     3661 cttagcatgc taaactagtt gagtcaagaa ttatttttca gttggatcaa tcaacattct
     3721 aatatttaat tagaaagaag cttggtcaac atggatacca ggtccaaatg ttgttgtgat
     3781 cgttacattt ttcatgtatt gacctttagc tgctgatggt ttagctttta gcaatacatc
     3841 atggattgct gcaaagtttt caactaattt ttcatcgtca aatgaaactt ttccgattgg
     3901 tacatggata tttccagctt tgtccacacg gtaagttact ttaccagctt tcacttcgtt
     3961 tactgctttt gttacgtcca ttgtaactgt accagtttta gggtttggca ttaagccttt
     4021 tggtcctaat actcgtccta gacgacctac agtagccatc atatcaggtg ttgcaacaac
     4081 tacatcaaag tcaaaccatc cgttttggat tttttgaacc atgtcgtcgt cacctacgaa
     4141 atctgcacca gctgcttcag cttcttttgc tttgtctcct ttagcaaaaa ctaaaacagt
     4201 ttgtgtttta ccagttccgt ttggtaatac aacagcacca cggatttgtt ggtctgcttt
     4261 tttagggtct acgttcaaac ggtatgcaac ttcaactgtt gcatcaaatt ttgcaatgtt
     4321 tgtttctttt gctaaagcta ctgcttcggc aacaggataa actttacttg aatcaacttt
     4381 ttttaatgca tcttgcattt ttttgctctt tttagccatt ttggttcctc cttgattgtg
     4441 gttataacgg ttgtacctcc cacgtgttct atatctttca atatagagcc aactgagaag
     4501 ctgctgtctt agggtgtata gattattcta cagtgatccc catgcttcgt gcagttcctt
     4561 caaccatgcg cattgctgct tcaacgttag ctgcgtttag gtcttccatt tttaattcag
     4621 cgatttctct tacttgatcg ctagaaactt tcgcaacttt atttttgttt ggttcgcctg
     4681 aacctttttc gattttagct gcttttttca atagaacagc agctggcggt gtttttgtaa
     4741 cgaatgtgaa tgaacggtct tcgtatacag aaataacaac cggaatgatc aaacctgctt
     4801 gatcagctgt acgagcgttg aattcttttg tgaatcccat gatattgata cccgcttgac
     4861 ctagtgcagg acctactggt ggagctggag ttgctttacc tgcaggaatt tgcaatttaa
     4921 cgattttttc tactttcttt gccacgagac atacctcctt aagtccgtga tgtggttaat
     4981 tggagatgca tatttctcct cccacgtttt atctgatctg aaaaaatcca gacaaataat
     5041 gtgcaaaaca atgcacactg gtacagtata gcaaatttat ttgttttttc aagatttttt
     5101 tctgctattt ttacattttg cttgaataaa cgctaaattt attctaaaaa aacaacaatt
     5161 tttcgttctc atttcttctt ttatgttaca cttgcgctag catggttcaa cagatgaaag
     5221 caaacaataa cacggttttt tagacccttg tacaaagatt cctacataac aaaatgaggt
     5281 ggagaaaatg aaagatttaa ggtatagaag tgtctttgat attattggac ctgtgatgat
     5341 tggtccaagt agctctcaca ctgccggagc tgctagaatc gggaaaatca tacgtagtat
     5401 atttggcgaa cagcctgatt ctgtagatat ttatctatat gaatcatttg caaaaaccta
     5461 tcgggggcac ggaacagata ttgcattagt tggcggcctg ttagggatgg aacccgatga
     5521 tgaacgttta gctgattctt tgaaattggc atatgaagcc ggtatggaag tcttgtttgt
     5581 tcctaagagt gaaaaagccg aacatccaaa ctctgtaaag atgcttctaa aaaaaggaac
     5641 acgaaagctt tcagtcactg gcatttcgat cggcggtggc aatatccaga tttctgaatt
     5701 gaatggattc aaaatttctt taagcatggg tactcctacc ttgatcgttg tccaccaaga
     5761 tgttccaggt atgattgcta aagtgactag tatactttct gagaccaata cgaatatcgg
     5821 cactatgaca gtgacacggg aatcaaaagg tgaaaatgcc attatgataa ttgaagtaga
     5881 tgatcctcaa gtagaagaaa cggtaaaaag actcaaacag ctgccaaata tcgacagcgt
     5941 aaattatttc gattaaggag ctaaaaaaat gtttttatcg attgaagaat tagtagatca
     6001 agcaaaagct tatccaagtg tagcagaatt gatgattgca gttgaaatgg aaatgagtgg
     6061 ccgttcccgt agtcacatca tcgagatcat ggaaaaaaac ctctcagtaa tggaacaatc
     6121 aatcaacgag ggaaaagaag gtgttacctc cgtcacagga attaccggtg gtgatgcaaa
     6181 aaaattagat gattatttaa atgaggcaga ctttttaagt ggagaaacaa tcttaaaagc
     6241 tgtacgcaat gctgtagctg ttaatgaagt caatgcaaaa atggggttga tttgtgctac
     6301 accgactgcc ggaagcgcgg gtgttgttcc tggtgtgctg atggctgtcc gagatcggct
     6361 tgatttgaca catgaacagc aattggaatt cttatttacc gctggcgctt tcgggttagt
     6421 catcgcaaac aacgcttcta tcagtggtgc agaaggggga tgtcaagcag aagttggttc
     6481 tgcaagtgca atggcagcag cagctttggt ttgtgcaaaa aatgggactc ctcatcaagc
     6541 agcacaagca gtagccatta cattgaaaaa tatgatggga ctgatttgcg atcccgttgc
     6601 aggcttagtt gaagtcccgt gtgtcaaacg taatgcttta ggctcttctc aagcttttat
     6661 ctctgctgat atggcattag cgggtatcga aagtgtcatt ccgccggatg aagtcgttgc
     6721 tgcaatgtac caagtcggac gtcaaatgcc gtcgattttc aaagaaacag cagaaggtgg
     6781 cttagctgtt acgccaactg ctaaacgact tgcggaagag attttaaata agagggcgtg
     6841 ataaagctcc ttaatttcga gtattaagag attagttatc ttcataacaa aaaacgaaga
     6901 gaggactaat tgcccttctc ttcgtttttt attaatccat caatttcgta ttttttactt
     6961 catcgatcgt ttttgttcct gcaagttgca tcgtgatcga caattctttg ttcaggtgtt
     7021 caaaaacaga tgttactcct tctgccccac ctagattcaa cccataaatc actggtcggc
     7081 caatcgcaac taagtcagca ccacttgcca gagctttgaa gacatgctct cctcgacgga
     7141 caccagagtc gaaaatcact ggtacacgtt tgttgactac ttcggcaatt tttggcaata
     7201 cttcaaagga cgcaggacct ccatctaatt gacgtccacc atgattggat acccagatac
     7261 catctgcccc tgcagagata gcgattactg catcttctgg cgattgtatt ccttttacga
     7321 taactggaag atgggtgatt tctttgattg ttttgatgtc atctggtgtc aagccttgtt
     7381 ttgcagctgc ataaatttct gcgattcctt ttccttcgcc attaccactt gcactttgtt
     7441 cgctatacgc agctaagtta ggcattggta atggaaattg gaattgattg atgacgtctt
     7501 cttcacggta accaccgagc gttgaatctg ccgttaaaat gatagcttta gcccctgctt
     7561 ctactgcttt gttcaaaatg aactcattga atctgtcgtc tttactcata tataattgga
     7621 aaaactgcgg tgcgcctggc gctgcatcgg ctgcgtcttt gattgtcgta tttgcataag
     7681 tgctgatgga aaagattgat ccagcagcag ctactccttt agctgtatca gcttcccctt
     7741 tttcatgtgc caatccttgg gccgctgatg gcgcttggat aatcggtgta tctaattcaa
     7801 ttccgaaaac agatgtatgc aaatcggctg agtcgattcc gcgtaatatc cgtggcatga
     7861 ttttctttgt attaaaagaa gtcgtatttt ctttcatcgt ccactcatct tcagaaccac
     7921 cgcgaatata gccaaaagct cctgcttcca tgcgctcttt tacacgacct tctagttagg
     7981 caatgtttac aatatcgatt ggatgttctt cattacttgt atgataagtc atagtagatc
     8041 cttctttcat cttcttgatt acatataaat catactaaac ttacaaaaag taagcaaaca
     8101 tttactctca gatactttta tcgctattct ttccaaataa atcgacttac aagctgatct
     8161 acttcttggt tttcatgaag catactatga gaggcatttt cacctgtcac tactttttcc
     8221 tctatttcat ttccgcgttg cttcaacaac gcatagaccg ctaatgcact gtttaacggt
     8281 actgttccat cactcaagtc tgtttcatct agttgaccag caatcaagaa aaatcgagtg
     8341 gttataggaa tattaccgat catttgctgg taatcttgat agcggttaga aacgacagca
     8401 ggcccctttg ctaactcatc tgtcagagac tgcgcactat catcgacaaa atcattaaaa
     8461 ggcgcgccaa ttgctgttaa tgtctgaact tgtggcaatg acgtatcctg tccatacgtt
     8521 cctaaatagc gtagtgcact aactcctccc atggaatgcc ccactatgtt gactttatct
     8581 atgccgtagt tttctttcag atacaaaaga caagttttga tccattctgc ttgattccac
     8641 tcgttatttt tattatcttc aaacaatact tggaccatcg gattgtctgc ttttccagac
     8701 aaatctcctg ttgcttgtat ttgtccatct gttcctactg ttaaaaccaa ttcttttact
     8761 gcttgctgat ttttttccaa acgccgcaac atccctttaa aggaattttc tgtcccgctg
     8821 tagccgtgga aaaaaagtgt tggaatagtg gttttttcta tcactttact ggattctgcc
     8881 gttttttctt tatcagaaac ttcagtagta tttatcttcg tcgtctggca accacttaac
     8941 gcaaacaaac tgataaggac taaagctgct gcagttgcac gtttgcataa atttttgatt
     9001 ggttctgtca tacacctctt cctttccata tagaaaaaag aaagacttga aaggccaatc
     9061 atatgatcga catttcaagt ctctttatta atcgatttta tctacttgat cgaaatcaag
     9121 ttctgtactg gtttcacgtc caaacatatc gatgttgact ttcaattttt ctttttctcc
     9181 gtcaacttct gttacttgtc cttctaatcc agcaaatgcg ccttcgatga ttttcacgac
     9241 ttcgccaact tctacttcta gatcattttg tcgcaggctc ataccgatag aacgcaagat
     9301 atgattgact tcttctggaa gtaatggtgc tggtttgctt ccggctccgt gtgaaccaac
     9361 gaatcctgtc acaccaggtg tgttgcggac aacataccat gaagcgtctg tcatgatcat
     9421 ttcaacaagt acgtatcctg ggaatgtttt atgaacgatt tctttttctt tcccattttt
     9481 gacttcagtt tctttttctt caggaacaac gacacggaaa ataaagtcgg ccattttcat
     9541 actttgcgca cgagattcga tatttgcttt tactttgttt tcatatcctg aatatgtatg
     9601 caagacatac cagttttttt caaaagattc cattgaaggc tcctcatttc attgtaaaat
     9661 aaaaaaacct cagtacaccc gaagtttttc tttcgcttca ttatagcact aaaagtacgt
     9721 tgtggctagc gagttatttt aaaatccagc caaataagct ttggatggct gtatccatta
     9781 cgtaaaacat caatccgaaa ataatagaag tttcgatgac tactaacgta tctttacgga
     9841 gttgttttct gcttggccaa gttacttgtt tgatttcatc ttttacgctt tttagaaatt
     9901 tcatgagaca acctcccttg ctactgttta tttcgtttct ttatgtaatg tatattgatt
     9961 acaatattta caaaacttat tgatttccaa acgttcgccg ctttttcctt cactgactga
    10021 ttttgtgtag ttgcgagacc cacaaacgga acaagcgagt gctgcttttt tctttgccat
    10081 ttacttctgc ccccttgctg agaatcagac ttatacttca taacagtatc acgtaacttt
    10141 ttacttgtca atttgtgaaa taaactatct attgttttgg ataaaattat gatatgatta
    10201 atttgaaatt aagctaaaat gagacaattg gaggtatagc gatgctatta aaaacacttg
    10261 tatataagaa aaaagatctg acaaccgtta cagagactag tacattagaa gaagcattaa
    10321 aaattttaga aacttccgga taccgttgtg tgccgatctt agattcttca ggtaatattt
    10381 tccgtggaaa catttacaaa atgcatatct atcgtcataa agcaaatggc ggtgatatga
    10441 cgcttcctgt cactcatctt ttgaaaaacg ctacaaagtt catttacctg gatacttctt
    10501 tcttcaaagt attctttacg atcaaagagc ttccttatat tgctgtcctt gacagtgaaa
    10561 atcattttta tggtattttg acacatagta cgttgcttaa tatgctctct caatcttgga
    10621 gcgtagaaca aggaagctat gtactgacaa ttgcttccac aggaaagcaa ggagatttgg
    10681 ctgctattgc aaaaatcatt tcaaaacaca gcagcattgc cagctgtatt actcttgata
    10741 tcgaaaaaga cgaatacatt cgacgtacat tgatcacgct accggctaac actgaagcga
    10801 aagtatgtga aacgatcgtc aaccacttag agaaaaagaa ttataaagtg gtagaaattg
    10861 aaaatttgca aaaccaagaa gcataatcaa aaagcgaaaa aagaaaacag ctaatctgct
    10921 ttcttttttc gctttttata attagacacg cttttcaaaa tcaagcgcgc gatctaaata
    10981 atacatcaat ggtgctgaaa cagccatgac gataaactca cttaatgcaa gcgtgccata
    11041 ggttgtccaa aatgcttggc cacctgttgg aactaacata taagcaatca gtcccatgct
    11101 gatggtaaag aagatcgtgt tcaatactaa acgcatcttt acatttttca ctttgttttg
    11161 caataaagct gtcaggccta aagaaagcaa tgtttggccg ccaccaaaaa ccacatccat
    11221 gataccaaaa ccaaagaagg cattatagac gatgaccccg cctaaaactc cccaaagcaa
    11281 tttacggtta aagacaacta aatgattcaa actttctgaa atacgaaact ggatcggccc
    11341 tgaggataca ggcgcaacta aaatagttaa cgctagataa agagccatga tgatgccatt
    11401 tacaacgatt acggataacc gttgtctgct tttcgttttt tgcattttct ctcctctttt
    11461 ctcctagttt ttttaacgcg ggatggttga tgaaccgcgg ttatgtgaaa caaacaataa
    11521 ttataacaga cttttcattt atttcaatcc gtcttatctg cttttaaaaa agcaaaagct
    11581 taaacaatgt aaacgtttgc taaaaatcgt gggatatggt ataaagtaag tgtaaggggt
    11641 cataagaatt cccagtaaag cagacgaagt aggatctgca agaacagaat tgttgtgaaa
    11701 cttaaataaa taaaaaagga tgtgggaatt tgagcaacct cttactagag aacggtctag
    11761 caagcagctg aagatctaag tggacatgcc gaaccttgga acttctcctt acgtaagtaa
    11821 tggatcatgc gcttgctagg cttttttgtg tttttcggtc ttcggacctt tttcattttc
    11881 ctagacttct gctataataa ccaagaatta attttgtaac agattttttt gaaaaaacaa
    11941 gtttctttca ttcaaaaaat agaaaaagct tgctaattat tggttaacca gcaaacagga
    12001 gtattaaaat atcttgtttt attgaatagc aggaggaaat catgaaattc cattatcata
    12061 tattgatcaa tccatcggca ggcagcggaa atggctataa agtagcagaa agaattttac
    12121 cggtattaaa aaataagcat attgactata cacttcatta tagtgaatac aaaggacacg
    12181 atgctgagat tgctgaaact ttggcaaaag aaacattgat tccttggata gaagaagaac
    12241 agaaaacaat cgatactttc ccccttttga tcatcgtagg aggtgacggc accttacatc
    12301 aggtacttga tactttctat cagatggaag tagaattccc agttgcttat attccagcag
    12361 gttctggaaa tgattttgct cgaggggcag gccttccaaa aaatcccaaa aaaggacttc
    12421 agctgatttt agcagctcaa tcaccagaaa aagtccacat aatggcttat gaagaaaaaa
    12481 taagtgaaaa aaaaggaata gctgttaata atttcggtat aggattagat gcagctatcg
    12541 tccatgcgac aaatcattct tccacaaaaa agcgattgaa caaatacaac ttaggatcat
    12601 tttcgtattt attctctatt ctccgtgctt tattcactca aaaaggattt ccaattctgg
    12661 tagatgcagg aggaaaacaa ttaagttttt caaacgcatt tttatgtaca gcaacgaagc
    12721 atcctttttt tggcggagga atcgcgattg ttccaactgc tgatgcgtct aaaccagtca
    12781 ttgattttgt cgtggtcgaa cgaatcaatt tctttaaaat cttgtggctc attcttttac
    12841 ttatacagaa aaaacaattg aaatcgaaat atttccatca ctacacaaca aacagattac
    12901 gtatcgtttc aacaacaccg caacatggtc aagaagatgg agaagaaatg gaaaaacgac
    12961 cttttgatat cacaataacc acaactaaac agttgttttg gtgttaataa aagaggtttt
    13021 gtcagaagct acatctggaa caccgtttat tcggaaaaaa ggactgtgac acgacttttg
    13081 tcacagtcct ttttttacta tgcagttaat tctttgccca attgggttgc catcgatagt
    13141 ttctttcttt acccaaaata aattggatca ttgaaaacac cgcagcaaaa gcaactaaga
    13201 cacaaccatt gaaccaaaaa gattctgcaa atgtttgatt gtaactgagt gtttcactga
    13261 tcaagttatt attttgaaca aaccaatatg ttggtgtgaa tgccgctatt ttgctaacca
    13321 tttctggcaa gtattctgct ggtacaaaca ccccaccgat aaagcaactg cctgtgataa
    13381 aaatatcttt gatcccaccg ctggtttctg agttttttac caatgaagtg atacaagccg
    13441 aaaagctaat gatcggcaat aaaaacaaga aagtatttaa aatatgatac cataccgtta
    13501 tatcccacga ggtgtggatc actaacagaa ccattagaag taagaagcca ctgaacaaca
    13561 gcagcgagta actaatcaaa ctaaaggtta ctttgcgatt caagtttcgt ttggtaatgg
    13621 gcgcactctg gtttcgttta ctgatctctt tgcgattaaa cgctagattg atgacgctga
    13681 aaccgctaaa aatggataga aacaacccat aagacaagaa attaaaggtt gtgcccgtca
    13741 acagcttttt catccgctga ctatatcccg cacttagctc aacttctcca gtaaccgaga
    13801 gtgccatttg tgtctgttcc atagctgcat cgattgtttt atcttcttct tgaaacagaa
    13861 tcagtgtttg caaaaaggtg ttcacttgct gtgtaacaac tgttttagaa aaggcatctg
    13921 ggcgactctg tatcttgatc gtcgccatct ttcctacttc gacttgttcg gtaaagtctg
    13981 caggcagatg taagataatg tttacacgat taaaataaag agcatcatcg atttcttttt
    14041 gtgaggttcc atccaatgta acgactgttt ccttgtcatt caaatattta cgaaggccat
    14101 cgactacagg cgactcttct tcactaatca aggcgatttt cgcatcttgc agagaggtat
    14161 tttcaacatc aatattttgc gcaaaaaaga atgtggcaac aacagtgata attacaccta
    14221 tcagtaaatt cgatttattt gctttaagca atgtccaaat cgttttataa agcgtcataa
    14281 cgcacccctc tttccatcca agcatttcct aacgcaaaaa ggaaaataaa tccgatcaac
    14341 catagaagat tggtataaaa actagccaaa tcagtgtaga aataaagttt atacaaactt
    14401 tcgctgatca agttgacgag atttaattga ccaacaatcg gaatatgaag atcaatccaa
    14461 tacttcactt gttccgaccc catcatacca gcaaaaaagc tcatcgcaat cgtcactgaa
    14521 atcaagatgc tatccttttg caccagattc atttttggta tcaaattacc aaccaatgta
    14581 cctaacaaaa tcgcacacaa cgaccccaga gcggtaacaa tcatcaacca ttgccaacgt
    14641 tcaccaaagt ctacttgata gataaaacga aagacagcta acataatata aatctctgtc
    14701 aaaacaatcg taaagcttgc tgccaaatta gcgattgaaa ctaacgattg ctttctagga
    14761 atcaaggaca agcgaatacc atttgacgat tgatttgctt gctgatcctg tacattgcgg
    14821 accccccaca tcgtcccgta gagaatcgcc attccaatca gagtgaagaa ataaaaactt
    14881 ttgaaactca tattacgacc tgcatgaact tcttgaaaag atacttcttg ttcaaaaaca
    14941 gtcatgatgg gcgcgagatc aacgttctcc acttctgcca ctttactcga aattatacca
    15001 atacgctgta aaaattgatc catgacgttt ttcatcaacg tctgactcat tcctgcttga
    15061 cccacatgga gcgaaacaga tgaaccatca atgagataaa aaccagccaa atcatctgca
    15121 tttagctttt cattcgcttc tacttccgat agttcctgta cttcaaataa tggtttatca
    15181 ttgacttcga cttgttctaa gactgtggta aaattttccg aaagcgctgt ttgatccgct
    15241 ttgattacaa ttccagtatt aatcgtttca agattgccac cttgatcaat ctcgccaaag
    15301 gccgccataa acatcaatcc taaaagaatt ggaaaggcca atgtccaaaa caacatggat
    15361 ttatttcgca gcagtatttt tgtgcgatat ttataaaaat gccaaaacat tatttcccct
    15421 cctctagtct ctcaaatcct tgccagtaat ctctaaaaag acatcattca aggacgcttc
    15481 tttggttgct atttttccgt aactgatctt ttcttcttgt aagattgtaa gaatcggcaa
    15541 caatactgta tttagttcag tagttgttac ttggagctga ccttttttat aagcgattcc
    15601 atcagaagct attgttcgct gaatcgctga caacacattt tctgggagta atgggatgtc
    15661 gatcgtcaaa cgatccgccg tccgtaccat atcttttaga gattctttgg ttcctttagc
    15721 aatcacctgt ccttgatcca tgatcacgat ctggtcgcaa agaatttcta cttcttccat
    15781 gtaatgtgtt gtatagacaa tggttgctcc ttgtcgattc agctccttga tactttctaa
    15841 aattttattc cgactttggg ggtctacagc tactgtcggc tcatctaaaa aaattaaccg
    15901 cggcttgtgg gcaatcccac atgcgatgtt caaccgacgt ttcaagccac cactaagttc
    15961 tcctggacga aacgaaacat atttttctaa tcccactaat tgaatcacct cttcaatcaa
    16021 ggctttcctt ttctcttttt cggaaacata taaaccgcaa aaataatcga tattttcttg
    16081 cacacttagt tcgtcaaaga ccgcaacgtc ttgaggaacg accccaatca ttgccttgac
    16141 agcataattt tcaggagtca ttgttgtccc gaacaattta atggttcctt tgtcataatt
    16201 taacaaagaa agcatacagt tgatggcagt cgatttccca ctgccgtttg gacctaacag
    16261 acctaagact tctccttctt gtacttgcag atcaaaatga ttcaatgcta caaaatctcc
    16321 atagcgtttg actaatttat caatttcgat aatcatcatg aaaaactcct tccaaatttc
    16381 ttgttgatct gactataata aagtgcaaga aaagaaacta gtgacgattg gctaaaccac
    16441 acatgacatt tgtcacaatg aaggagtctg ctatgcataa atctcggcta ctcaacgaaa
    16501 ctagctttct actggttatc tcattgcttt tgctggtaag tcaaggattc gttgctatgc
    16561 aagttatcta tttattgatc gccgttttat tcactggtgt cagttattta ctgcctacaa
    16621 agccaaattg gattttccaa gttattctat ttcttttatg tgccttttta cctgatcttg
    16681 tcctgtttct gccagcgacg atgcgagtga tcgcccctga agaaagccac cttgattact
    16741 tgtttttgtt aggttttttg ttgatttcga cggttggtaa tcatcccccc atcaaggtcg
    16801 cctttgtcgt aattttgctg tttttttcct tttacttacg aaaaaaagat caacgaacga
    16861 ccgatcttga gaaaaagtac attgtctcca aagatactca ttgggaaaaa caacaagagt
    16921 tatcggaaca aaataaagca ttggccctgt cacaagaaac acaattagcc ttgcaaattg
    16981 tggaagaacg gaaccgaatt gcccgagaca tccatgacaa tgtcggtcat ctgctgtcaa
    17041 gtgcgatttt acaaattggt gcgcttgaaa tgatcaatca agaaccaaaa ctaaaagaac
    17101 cattagaaaa tttaagtgcc accgttcaca ctgggatgga tcggattcgt caaagtgtcc
    17161 atgacctcca tgagacatcg atctcttttc aacactcctt agagtttctg atcgctgatt
    17221 ttccttcttc tgttacgatt gaaggaaact tgtttgaagg attgaatgaa acgcagcaaa
    17281 actgcttctt aatggtcatc aaagaagcct taaccaacag tatcaaacat agtcatgcca
    17341 aaaccgttgt tttacatttt cgaacgttgc cggcctttta tcgcttgcaa atcacgaatg
    17401 atggcaccag cccacaaaaa aattctgctc ccggtattgg tttgaaaacg atgcgtcaac
    17461 gtgttcaaga acaaaaggga caagtccata ttttccaaga aaaccagcaa tttcagttaa
    17521 cgatcatttt accaagggag ggacaccaat gataacagta gtcattattg atgatgatcc
    17581 atttgtgacg aaatcgttac aaaccatttt agaaagcacg aaagagatcc gtgtgttagg
    17641 cattggtcat tgtgccaagg atgcgctagt cttatacgaa acccatcgtc ccgatgtttt
    17701 gctgacggac atccgcatgc ctaaacaaac gggcatcgat gctgccaaag aaattttggc
    17761 taaatttccc aaagccatcc ttttgctttt gacgacattc aaagatgaag aatacatccg
    17821 cgaagcattt tccatcggtg tcaaagggta tttaatcaaa cagaacttgc aagcgattat
    17881 tccttctgtc aaatccgctt ataatggaca ggccgtattt ggtaatgaaa tcatcgagac
    17941 tttcactcaa atgatgaaga ataaaccgac gattgatagt catgcttatg cttcttttag
    18001 tgaacgtgag cttgcaatca tcaaagaagt agctgccggg aaaaacaata aagaaattgc
    18061 agatgccttg tacttgagtg acggcactgt tcgcaattac atcagccaat tactagaaaa
    18121 gctagattta cgggatcgca cgcaattggc tatctatttt tatcagcatt tgcaataagt
    18181 cttatctaac acaaaaaaac ctgaatgatg ctccttcgca tcattcaggt ttttttcaca
    18241 tcttctattc aattgtcccg caaaccattt cctcacgatt gaaagaatca aggattcgca
    18301 gggctgccag gattcgttgg attgttcggc gtgttaggcg gaaaaatagg cgccttgtct
    18361 aacgtttttt ctagttttga ttcttcacga tcgtctcgtt ctacatcagg actttcattc
    18421 aatttgaatt ctttttccat ctcatctaac tttttaaagt cattgccaga ttcttttgct
    18481 aatttcgcta atttatcttc ggtttcttta gccataaagt aacgcccccc ttttgatttc
    18541 agcttagcat atcacactga aaaaacaaaa gaaaacgcta accaacgatc atttttagct
    18601 atttctcatc tttaaaatca agcatttttt gccggtataa aatcgtaggg agaactgttt
    18661 tccataccaa tcatcggatc tactaactgg ttatcgatca cttctgctgt aagaacgaaa
    18721 tctttagaaa cagcaggttc tacctcttct ttcttttctt ctccaacaaa tgaagaaaac
    18781 agatctggtt ccactgtttc cattacttgg tttttcgttt cattcgtccc actgtcatct
    18841 tctacaggta gcttttcttt tacaacccgt ggctcttttg tatagttccc atcttcaaat
    18901 ggatcatttc cgaccaaaga atcttcataa gctgctagtt tggtccggag agtgatattc
    18961 attttatcat tatccatgat ccttgatttc aacgtagtca tccggcttgc tgattcattc
    19021 gctacacaag tgacataggc ttgctcttcc cctaacaaaa tcaacagttc cttactgcga
    19081 gtcactgccg tgtagagcaa attacgctga agcatccgct gatattgatg gaccatgggc
    19141 aaaataacca tcttgaattc actgccttgt gatttatgga ttgagcaaca ataagaaaga
    19201 gtgattttat tccattcgtt tcttttataa gtgacctcat ttgcatcaaa ctggataact
    19261 aattcatcca ctttatcttc agaatctttt gccaagatga tacccgtgat ttctcccata
    19321 tctccattga atacattcaa ttcgggagta ttgactaatt ggaggacttt gtctcctatc
    19381 cgataaacgg tgtcgttcca atgtacttcc ttttttccag gtttccctgg attgaatatc
    19441 tcctgcatca ttttatttaa cgcatcgatc cctgcaggtc cccgatacat tggtgctaat
    19501 acttggatat cttgtgcacg gaatccctta tttttcgctt tttccacaat tttagagatc
    19561 aagggttcaa tttgataagc atcacttcgg aaataggaac gatctttttg attctttgtg
    19621 aaatcttttg gcagacgtcc ttctttgatc tcatgggcaa gaggaataat actagaacca
    19681 tcaccttgcc gataaatctg attgagttcc attttgggaa tttgttcgat ttccaaaaga
    19741 tcatggagta cttgaccagg accgacagag ggaagctgat ctttgtctcc tactaagata
    19801 acttgcatat tcgtcggaat cgctttgaat aatgtgttag ccagccatgt atcgaccata
    19861 gacatctcat caacaatcaa aagtccgcct tctaattctt tcgtactgac tgaaggtgtt
    19921 ttttcccgtc cattcagccc cagaagacga tggatcgtac tggcaggcag acctgtcgtt
    19981 tcgttcattc gtttagctgc tcggccagtt ggtgctgcaa gtagaatagg aaaaatttct
    20041 tgggtatatt ttgaaggatc caattccagt ccgttcaact ctgcaaaaag attgacgatt
    20101 ccgttgatca cagtagtttt ccccgtacct ggtcctcctg ttaaaataaa tagaggggaa
    20161 cggattgctt cagcgatagc ttgttcttgc gaaggtccat actgtatgcc gaatattttt
    20221 tcgatacgcc gaatattctt ctgcacttct ttttcaggat aatggatttc ttttttacga
    20281 ctgagaaggc gctggatcga attaccgatc ccccattcag aaaagtacaa actgttttcg
    20341 taaagattag tctcttcttg ttgtatcttt ccttcttcaa caagttggat aattacagta
    20401 gcaacctgat caggctggat ttcaattggg cgacttgttt ctaaaatacg gatcgtttgc
    20461 tgaagcaatg tctccgccgg tacgtatgta tctcctgttt gaactgcatg ctgaaaaatc
    20521 tgatgcaaga tagctgctcg aattcttctc ggtgaatctg ctgcaatacc gatttgttcg
    20581 gctagattat ctgcacgttt gaacccgatt ccttctatgt cttcgactag ctggtacgga
    20641 ttttcttcga tgatatccaa agactcgttc ttataggcct ggtagatact gaaagctaac
    20701 tgactaccga acccgtaacg attcagtccc atgatcactt gatccatccc gtggtttagc
    20761 cgaatcgtct cagcaagcat ttctcttttt ggcttcgtca aaccggagat ttgttttaat
    20821 accgaaggat cagcaatgat tttttcgata gcatcttctc ccagcaattc tacaattttt
    20881 tctgctgttt tcttcccgat tcctgggaat ttttcgctgg aaagatacgc tacgatccca
    20941 tttgcagttg ttggcttttc ttgctcatat ctttcgagtt taagctgcct tccgtattta
    21001 ggatgatcca ctaattctcc ataaaaacga taagtctcat tttcttgaat atcgccgaaa
    21061 cttccggtga ctacaatctc tttttcacgg taatcagctg tggtttcact gatgtgtacc
    21121 aaaagcactt tgtaaaaatt actaggattt tgaaaaaaaa tcgcggcaac tgtaccaacg
    21181 aaataaggca tttcttctgg ctgcatcgga agccgcctcc ttcttctata gtaaaggttc
    21241 tggtgctgtt tcagttccta aaaaatctgt ttggttccat actttcagtt catatggcaa
    21301 taatctgcaa ggctcttcag tctctaaaat cgtcaaacta ctattgaaca atccacccat
    21361 ttcacgcaac tcacttagct cttttcctgt catccattga atggctgcag ttaaagaagc
    21421 tccatgtcct acgaataaaa gtgggccatc tttatgtcgg gcaaccgcat ctgctacaac
    21481 agtttcgata cgtgtgattg ccgattcaat cgtttctccg tgaaaaggag aaggatcata
    21541 cagatctaaa tgattacgca aattcggcag attatccgga tatttctggt acatttcatc
    21601 gatcgactga ccttctaaag tacccagccc tagttctctt agcttatctg tataaaaaat
    21661 ctctaccgga cgagcaagct ctttgttgat accttctgct gtatcacgtg ctcgctttgc
    21721 tgtactggaa taaattgctt taaaaggaac atattttacc tttttcccaa atgctttgat
    21781 ttcttcgtaa ctttgtggca aaagcggaga atcgccattg ctcccttgaa aacgtctttc
    21841 ctgattccat tgtgtcttcc catgtcgggt aaaatatagt tccatacgca gcaaactcct
    21901 gtctttcttt tattttactc tataataaac tgtctaatcc agcttgtcat tttcatagtg
    21961 atgcgtcaat tctaaattag ggaatccttg ctggcgaagt gcttcataca ctatcattgc
    22021 agctgtattc gataaattca atgagcggac atgctcatca ttcataggga tgcgtaaaca
    22081 cttttcttcg ttttcacgca taaatgtttc tggcaaaccg gttgtttctt ttccaaataa
    22141 aaagaagtgc tcttctccat cagcaaaatt ttcgtcacta tatactttat gggcaaattt
    22201 tgagatcaaa tgtagtttac gattacctaa aaatgttaaa aaagcttcta aattactgtg
    22261 gtaagtaata tttacatcat tccaataatc caaccctgcc cgttttagat gtttgtcatc
    22321 agttgaaaaa cctagtggct cgattaaatg aagcggagta ttcgtggctg cacacgtacg
    22381 ggcaatattt ccagtgtttg cagggattaa tggttcaaat aaaacgatat gattcatcag
    22441 gatcattcct ctcatattta tgttcgtttc tattttctga caatttgatt tttgcataaa
    22501 aaaaaacgta tcaactcggt gtcagtcact gcgagtcgtt acgttagtga agctcttctc
    22561 actaactttt atcagaacag gaacctgata aaaaccaatg aaaaaacaac ttgttgctaa
    22621 tataacaccc tcaacttttt tttgcaacct ctactcatta aaaactgcta gaaaaacgtt
    22681 ttctcttagt tccttttaat aattctatgt caattgtaat cttcttcaaa ggattcttat
    22741 ataagtctgc tttgattcgc tcttctacct gccattctaa aggtgacatg gccaaaagat
    22801 ctttttgatt ttcctctggt atctcaaatt cataggttac acgctgacgt tcagttaatt
    22861 tcattgcttc agcaaacttg gctactacac gttcattgga ataactctgt ttttcaggtt
    22921 gattcggata aaaagcctga cgtagttcct ttaaataatt ttcttgtggc accaccttca
    22981 acacttctcc atcttctgag agtacacgtt ggaattcttg gtaatgagaa ggcgaaaaga
    23041 tatttaaaac cgcgttgaat gtttgatcag caaacggcaa attggtcaaa tcagctacac
    23101 accaaaatgc ttcggcagat tgttctgtag ctgcgtagac tccttctttt gaaatatcaa
    23161 atccaacagc attttgaatc tttttcaatt ttgaaagttc attcaagaag ctaccttctc
    23221 cgcagccaac atctaatagc gagacttctt caggtaaatg ctgcgcaata gtctcaataa
    23281 cgggctgata catgccagat tggatcattc tacgccttgg aacgaacatt ccttgatcgt
    23341 attctgtttt aaccgcatgg gataaaaaat aaagtgttcc ttttttggaa aggtcaaaac
    23401 ggtgattatt tttgcatatc aagccattat cagacttttt tacttcttct tggcaaaccg
    23461 gacagcggaa cagctgatga ttttctgcca aaaactggcg tcctcggtca atttttttta
    23521 acatagttcc ccctcatttc tgttttatct taccgttaga tgctcacttt ggcaactgct
    23581 aaaaatatga tacaatgcct agaagaaagt gaaagataaa ggagaagatt agatgatcaa
    23641 agaattttgc gctgaaaact acacatctat tcctctagca attgctagag gagccgatcg
    23701 gatcgaattg tgtgacaact tagcggtcgg tggcacaacc ccgagtactg gtgtgatcga
    23761 agaaacgtta agttatgctg gggaaaaaga tatccccgta atgacgatta ttcgtcctag
    23821 agggggcgac ttcgtctata acgatatcga attgaaaatc atgcatactg atttgattga
    23881 agcaaagaaa ttagggacgg atggcgtagt atttggttgt cttactcctt caggctggct
    23941 agacgaagag gccttagaac tattgatcga aacagcagaa gggttgcaga tcaccttcca
    24001 tatggctttt gacagtattc ctgaagatcg gcaattcgaa gcaatcgatt ggctggtaga
    24061 acatggtgtt gaccgtatct tgactcatgg cggtcctgct gacaaaacta ttgtagaaaa
    24121 tttcccacgg cttaaagaat tgattgccta tacagatggc cgcttaatca ttttgcctgg
    24181 tggtggtgtc actgcagaga atgcagcaca tgtagctaaa gaattgaacg taagcgaagt
    24241 acacggaacc aaaatcgtgg atcttcagtt ataatcttat aaacaaaaaa acatttgcaa
    24301 caggcataat gcttattgca aatgtttttg tttttttaca taaaatagcc cataaataaa
    24361 gaagcaatat tgaaataaat cagtacactc gtcaaatcgc tcaaagtcgt gataaaggga
    24421 ccgcttgcta ctgctggatc aaatcctaga cgatccatta gcatcgggat taaactacct
    24481 gcaagattcg ccactgtgat tgcaaatagc atagccatcc ctatgacaaa ccccaagata
    24541 aaattatgtt tccaaaaacc tacgattaga aaaatcgtga tccccgtaac agctcctgtg
    24601 acaacgccgg tcaagacttc agccaaaaca agtcgaacaa atccatgatc tttctcatca
    24661 gaaatcgcaa gtcgacgaac agccacagct agcgactggg ttccagcatt accggcagtt
    24721 cctgtaatca gcgaaatgaa gactgctaaa atactcgctt cacttaccaa gtcttcatag
    24781 tgactgatta aagaagcggt cgacatacct aaaaacaaca gagtgatcag ccaaggcaaa
    24841 cgtcgagacg ctgcctttac tggattttca ttaatttctt ccacattgac cccggcaagt
    24901 cctgaataat cgctagcagc ttcatcatca ataacgtcga tgatatcatc gactgtgacg
    24961 attcctaaca aatgatcatc ataatcggta acaggaagag ctaagaagtc ataatctcta
    25021 aatgtttgtg ccacatcttc ttggtcgtcg cctacatgaa ctgaaagcac tcgttcgctc
    25081 atcagatcag aaatcattgc gtcgtcatca ttcacgatta ggtcgcgcag agaaataacc
    25141 cctactagct gaccttcttg attaacgaca tagatgtaat agattgtttc agctacatct
    25201 gcttcgttct tcagcacgta catcgcagag cgaacagttt gattggctac gatcgaaaca
    25261 aattcagttg tcatgatcgc gccagccgtt tcatcctcgt aatggagaag ttccttgatt
    25321 tcactggcat cgtctgccga catcaaactt aaatatttag caatttgctt tttatccagc
    25381 gtattaagca agtcaacagc attatctgta tacatctcag aaagcatatc agctgcatag
    25441 ctaggacgca tttcatctag gtattctttc atatgttcgt tgtcttcttc aatcacatcg
    25501 aacatatccg ctaattcttt aggcgacaga taggaataga tcagctgcct gttctcttct
    25561 gtaagagatt ggtagaattg cccctgctca tagatatgca tctctaaaaa actgtttctg
    25621 aatgcctgaa tattattttc ccttaaatct tgagtcaact gggcaaatct ttcctctaat
    25681 tcttgatttt catccaaacc aatctcacct cgccttattc ttcaaaccgt ctcacaactg
    25741 cctccatgtc tgcggctaaa ggctgcttga actccagcaa ctcttctgta aatggatggt
    25801 aaaaacgcag atcataacaa tgaagggctt gtctttttat tcccagatcc attcgcccgc
    25861 catacatatc atcaccgagc agggcacagc caattgcgga aaaatgaact cttatttgat
    25921 gtgtccttcc tgtatgcagc tggatatcaa ctaacgcgat ttctgttagt cgcttctcta
    25981 tccagtactc agtctgtgcc gaacgtcctt catcagagac cattcgcttc aatagcgaag
    26041 aataatctcg ggcgatcggc aaatcgatat gtccatgtgc cgataaatct gaaagccttc
    26101 cactgactaa ggcctgatat tttttcacaa acttcttttc acgaagttgc atatctaatt
    26161 ttgcgtgcgc aaatccatgc tttgcaaaaa gcatcaagcc agatgtatcc cgatctagtc
    26221 gcgtcaccac atggattact tggttttcgt aaccttgttt tttataataa gcctttacac
    26281 gattagccat tgtcccattt ggatggtatt gggcaggaat agacgaaacg cctgctggtt
    26341 tatttactac cagaacgtgt tcatcttcat acacgatatc gatcggtgtt tcatccaaaa
    26401 gtacggtttc atgttctcct tcagcaggaa tcacaatggt taccttatca tccttagcta
    26461 gagaaaacaa gacattttct acttgatcat taactttgat ttgaccgcct tgaaatttta
    26521 ttttcgctaa cagacccttt gagatccctt tttcttttaa aaaatatttt acttgctgag
    26581 aatgatcttt ctcataatcc catgtaaact gcatatcaat cctcactaat aaaggcatct
    26641 ttcacacgat gccaaaaatg catatgtcga taagaagcaa aatggatccg ttcttctgct
    26701 attcgataat aaattgagcg gatatttgct tttgcgacat tcaattgatc aaccgttaca
    26761 agataatctt ctgtattttc caattttatt tctagccatt catcatgggc aatcacgatc
    26821 ggagaaccca aagtacgaaa cactcgatta ttcagtgaag caatctctgc caactgaaag
    26881 gcattgatac taggatggat cacagcaccg ccgacacttt tattatatgc tgtcgaacca
    26941 gtaggcgtag aaatcgtcaa tccatctcct ctaaaacttt caaacagctc gtttttgatg
    27001 tatacatcgg ctaccatcgt acgattggcc cgtttaatcg ttgattcatt cagtgctaaa
    27061 aaatgctggt ccggtgtttc atccaaataa ctgatccgaa catccagcaa gggataacta
    27121 acactttgtt cccggttcgt tcgtaagctg tctactagtt cttccagctc gtaatcccgc
    27181 caatccgtgt aaaaccctaa atgacctgta tgcacgccca agaaacgcac atcattcaga
    27241 caatgactgt agtgatggaa cgccgacaat aatgtgccgt ctccgccgac tgagatgacc
    27301 aattctggct catgatcatc tcgttgaatc cctgcttgtt ctaaaagtgc gttcaaacgt
    27361 tctgtcactt ctatagtctt tgcttcttta ttatgaacga tcgctacttt catcccctgc
    27421 ctcctctctt tcgctttctt tcttcaaaag cgttcgatag tagacatcat ctgattttcc
    27481 tcttccatgg gagaataacc gctgtgcttc ttggatttct tcacggatac tggacatctc
    27541 ttcatccaac tgataggcag cttctgccgc acgctgaagt cgttcactca tttcttctgg
    27601 aaaaacgcct tgatatttat aattcagcga atgctcaatc gttgcccaga aattcatcgc
    27661 aagagtccga atctggattt ccgccaaaat tttcttctca ccgctgatca gctgtacagg
    27721 atactcgatc accagatgat aagaacggta gccacttgct tttttatttg tgatgtaatc
    27781 tcgttcttgg agaactttca tgtcttttct tttacggatg atctcaacaa cttgatggat
    27841 atcttctaca aactgacaca tgatccttaa cccggcaata tcagacattt cttcttctaa
    27901 acgtcccatt ggaatatgcc gtaattgtga ttttgttaca atactatcga ctggcttgac
    27961 tctcccagtg acaaattcaa tcgggacatg tcgattttgt tcacggaact gtttgcggat
    28021 cccccgcagt ttcactttca attctgatac agtttgttca tatggtgcta aaaatgcttc
    28081 ccaatctcgt tccatagctg atacctctac ttcattaaca ttcattgttt attctaccat
    28141 atatgcaaag ttttttcgta ctcttgattt atgcattgac caaaaaaaga aaattaggca
    28201 aaatagacaa aaaggagtcg atcaaatgac acaaatgctt gaaatcgaat ataaatctat
    28261 gctgacaaag caagagtacg agatattcat cgctcactat caattaacag ataaagattt
    28321 ccgtgtacag actaatattt atttcgatac cgttgatgaa cagctgaaga aacaaaattg
    28381 tggcctacgg atccgtttcg ttggtcatca agctgaatat acattaaaaa cacctgctga
    28441 aaaagggcgt ctcgaaacaa cggatacctt tataacagaa gaagcagaag catttatctt
    28501 cgaaaaacgt ctgcctcgtt caggcgcagt ttttcaaaaa ctttctgatt tgaatatcga
    28561 tcctgcttca ttaatccaaa ccggaaagct tacaacaaaa cgagctgaat tccctattga
    28621 agaaggactt ttagctattg atgaaagctg gggagacaat ctacacgatt ttgaactgga
    28681 actagaggta ggggatgcat ctgtgggaaa aattgctttt ataaattttt taaaacgttt
    28741 ttctttacct tatcgtcctg ctaagaacaa gatccaacgt atgcttgaag ctaaaaatta
    28801 gaaacctttc attttcacaa ggcacttgag ttcatttttt tacttttttc tgttaagtta
    28861 taggatgtga aagaatctag tcactagttg tatagaatct attttggggg agattcatat
    28921 gatcgaaatc tatttatttg ttaatccttt aggggaatac tgtttcgaaa tggaacaaca
    28981 gttattacaa tttatcgaag aagagtacgg gacaccatca aaagaaaaga tgcaatttcg
    29041 ctttcttcct ttggtcaatt tgcagacaat tggggatgtc atgcaacaaa aaggaatttc
    29101 tcagaacaat cttgaggaac gtaatcactt attctcaatg acttattctg cagcattgga
    29161 ctgcaaagcg gcgcagtttc aaggaaagaa aaaaggccgc cgttttttga tcaaattgca
    29221 agaagcagtc ggctgtaatg acataccata ttctcaagaa cttgctgaat ccatatttga
    29281 agaaatcggc ggcgatttga atatgtttaa agaagatcgt cagtctgact tcgttaaaga
    29341 agtattcttt tctgatcaaa aaattgctcg ggaaatgggc atcacaaaac acccttctgc
    29401 agtcgtttac aattatgcct gcgaccgtga ttttggtgtt ttagtagaag taaaaggtac
    29461 aagtagcctt tggagaatca cagagctgtg taaaacaaca gataactcat atcaagtttt
    29521 taatggtgaa catttacata caaatgattc tcagttccat tcaagaacta gccatttaca
    29581 cttgctttct aa
//
LOCUS       DACDEG010000024        27573 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.024, whole genome shotgun sequence.
ACCESSION   DACDEG010000024 DACDEG010000000
VERSION     DACDEG010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.024"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(136..1080)
                     /locus_tag="IV142_001169"
     CDS             complement(136..1080)
                     /locus_tag="IV142_001169"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP8484839.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1376..2178)
                     /locus_tag="IV142_001170"
                     /pseudo
     CDS             complement(1376..2178)
                     /locus_tag="IV142_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            2399..3613
                     /locus_tag="IV142_001171"
     CDS             2399..3613
                     /locus_tag="IV142_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8484840.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3635..5650
                     /locus_tag="IV142_001172"
     CDS             3635..5650
                     /locus_tag="IV142_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP8484841.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5733..7106)
                     /locus_tag="IV142_001173"
     CDS             complement(5733..7106)
                     /locus_tag="IV142_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP8484842.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7132..7416)
                     /locus_tag="IV142_001174"
     CDS             complement(7132..7416)
                     /locus_tag="IV142_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8484843.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7418..9433)
                     /locus_tag="IV142_001175"
     CDS             complement(7418..9433)
                     /locus_tag="IV142_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8484844.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9612..9917)
                     /locus_tag="IV142_001176"
     CDS             complement(9612..9917)
                     /locus_tag="IV142_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP8484845.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10006..11037)
                     /locus_tag="IV142_001177"
     CDS             complement(10006..11037)
                     /locus_tag="IV142_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP8484846.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11244..12119
                     /locus_tag="IV142_001178"
     CDS             11244..12119
                     /locus_tag="IV142_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8484847.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12271..13113)
                     /locus_tag="IV142_001179"
     CDS             complement(12271..13113)
                     /locus_tag="IV142_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8484848.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13205..15082)
                     /locus_tag="IV142_001180"
     CDS             complement(13205..15082)
                     /locus_tag="IV142_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8484849.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVCNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15079..16500)
                     /locus_tag="IV142_001181"
     CDS             complement(15079..16500)
                     /locus_tag="IV142_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8484850.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16503..19892)
                     /locus_tag="IV142_001182"
     CDS             complement(16503..19892)
                     /locus_tag="IV142_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP8484851.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20149..21540
                     /locus_tag="IV142_001183"
     CDS             20149..21540
                     /locus_tag="IV142_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8484852.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21613..22260)
                     /locus_tag="IV142_001184"
     CDS             complement(21613..22260)
                     /locus_tag="IV142_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8484853.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22268..22954)
                     /locus_tag="IV142_001185"
     CDS             complement(22268..22954)
                     /locus_tag="IV142_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8484854.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(22960..24033)
                     /locus_tag="IV142_001186"
     CDS             complement(22960..24033)
                     /locus_tag="IV142_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP8484855.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24255..24929
                     /locus_tag="IV142_001187"
     CDS             24255..24929
                     /locus_tag="IV142_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8484856.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            24934..26274
                     /locus_tag="IV142_001188"
     CDS             24934..26274
                     /locus_tag="IV142_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8484857.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26423..27409)
                     /locus_tag="IV142_001189"
     CDS             complement(26423..27409)
                     /locus_tag="IV142_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP8484858.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8039 a         5201 c         5016 g         9317 t
ORIGIN      
        1 cggatacatt ttcggaccga ttaattttaa ccattctgtt tatttgctta cctattagat
       61 atcaataaaa aactgcttaa cagctatttc agctgttaag cagttttagg atttttagtt
      121 catattcatt gtttcttata atacagtgtt caatacttct cttaaagcac ttgttgattt
      181 gctgaacatt gctttttctt cttcgttgat tgataattca acaacttcac gaacaccatt
      241 ttcgtcaacg attgatggca cacctgtaaa gatatctttt tcgtcgtatt caccagttaa
      301 ataagcagat acaggcaata cagcttgttc gttattcaag atagctttta cgatacgtgc
      361 agtactcata ccaataccat agtaagttgc ttttttacga tcaatgatct cataagcagc
      421 attacggact ttatcagcaa tcacatctaa ttcatcttgg gcaatacgat ggtctttttc
      481 tacaatttca aacactggtt taccaccgac agttgtatgt gaccatgcag caacttctga
      541 atcaccatgt tcacctaaga tatagccgtg gacgctgcgt gggtcaactt ttaatttcaa
      601 tgcgatttct ttacggaaac gagttgtatc caatgttgtt ccagtaccga taacacgtga
      661 tgttggcaga ccagattcgt tccaagcgat atatgtcaaa atatctactg ggtttgaagc
      721 aacaacgata ataccgtcga atcctgatcc catgatttct tttacgattt ggcgcatgat
      781 tgacgcattt gttttaacta aatctaaacg agattgacca ggtttttggt tgataccagc
      841 tgtgataaca acgatatcag catctttaca ttcttcatag ccacctgacc atacagccac
      901 attttcatcg ccccaagcca taccatctaa aaggtccaat gcttcccctt ctgctttttc
      961 ttgattcaca tcgatcaata ctaattcatt agagattcct tggttgatca ttgcataagc
     1021 gatacttgtt ccaacaaaac ctgtaccgac gattactact ttacgacttg tttttttcat
     1081 gaataaacag ctccttttca taactatttg ttactttcat gaaaagtata gtcttgttta
     1141 ttcattttat caacatgtta attataatag ttgttattat aatttttgat ggaaacgaat
     1201 tcctgaatac gcataacaga taaatgacaa atgtttacac ctatatcccc tgataataca
     1261 accaaaagtt tagaactgaa aataaatcca aaaaaattcg ctgcaacgat ataataagac
     1321 agcaatcgca cctaaaacaa ttttccttca tatgctttag tgaaagattt tcttcttaat
     1381 gtttatttct ccattcattg ggagtacatg ttgaatactt cttgaataat ttgaataaag
     1441 aagtcgtttg tttatatccg atacgattag cgatttcttc caacgaaaaa ctcgtttctt
     1501 gaatcagttc caacgcaaag tcgaattttt tctttagaac cagttcttga aaggaagcat
     1561 caaaatacat tttcaattta ttactaagat agaccggatt gtagccaaaa tgagctgctg
     1621 tttcttttaa cgtacaagaa gtaaaatgct tatcgatata atctaatact ctaagcatct
     1681 cattttctgc gcatccatct gctgatgaaa agctttgttg ttctaataaa tgagtcaatt
     1741 cgatcagtaa tagtgatgta tagagattta acgaacggat cttatgactg tatgtatcaa
     1801 aataaatcaa cagcatattt tcaatcaaat gcgtcaaacg acttgttttt ccgccgcgaa
     1861 aatgaatgaa ttgtgtatgt tccccttttt cttttgatgc atttatcaaa aaagaagaaa
     1921 gtagattatt tgtgcttgcc aaatgattca atatctctgt agataaggtt tcttctttta
     1981 ataagatgtt gattaaaata tcctcttgcc ctgtgtattc gattctttga ggtgtttact
     2041 gatatgaata tttccctgtt gaaatagatc ccctcggtgg atttttggcg gtgtctgaag
     2101 atggtaagct aagtttaaat cattgatatt cattccttgt tcacgctgca gagtttcgat
     2161 tgattcttcg ttacgcaaaa aaaatgtcta attcagctag ttccaattga ctccctcctt
     2221 tttgtaagcg gtttctttcg cttattagtt cacgctgtac tgctattata tgcaaagcta
     2281 tttttggttt cataaagact ttctatggtt gggacatgaa cttcctgtga ttccgctatt
     2341 gagagaataa ccacttcata ctatattttt gataagcgct tacaaaaatg aaaggaacat
     2401 gcttatgaat aaaaagatga tgaaattttc tctgctgtca gtctctctta ttttgacttc
     2461 tgctggtgca gtcacgtctg caattcccag tatgtcagct acttatgcca cgcgttcttt
     2521 atctgctgtt gaattattaa cgactgctcc ctcttttatg gtcactattt ttgttttgat
     2581 tagtagtttt attgctagga aaatcggaca aaaacaaact gtgatcatcg gattagtgat
     2641 tgcatcgatt tgtggtatcc tccccctctt ctcacaaagt tacagtattg tcttagcttc
     2701 aagagctgga ctaggtattg gctttggatt gatcaattct ttggcagtca gcatgattgc
     2761 tgccttttat cacggagatg aacgagcctc tatgattggt tttcaaagtg catttcaagg
     2821 atttggtgca gctttaatga catttgttgc aggacagcta gtacagttcg gttggcaata
     2881 taccttttta gtctacgcca tcagcttacc tattcttgtc ttgtttgttt tgtttgttcc
     2941 tacacctgaa acaactgaac ttcaatcgca tggcacagcc ccaacaagac aagtaatcaa
     3001 aacgaatcct aaaatgatta gttatggtat ttttctcttg ctggtcatcg tggtatataa
     3061 tgctgtatcg attaaattag ccacagtcct gacgacttat tctctaggaa acgaaactaa
     3121 tgcagcgaat atcttaacaa tcatgcaatt ggcaagtatg gctgccggag tttgttttgg
     3181 ctggcttcag acaaaagcac atcgctatat gcttacactt tctttactac tgatgggaat
     3241 cggctttgta ttgattgcca ctgtgataaa cctgtatgtg attgggtttg gtgcgatcct
     3301 aacaggaatt tctttttcgt tattcattcc ttacttattc aaccaagtaa gtatccaagc
     3361 gcccaagtac gcggagagtt tcaatacttc aatcttattg gttggcgcta atttaggtac
     3421 tttcattgca ccatatggtt tgctctttct ctcttttgct tctcttggga gtaaaacact
     3481 tgctgttttc gtcaatggca gtatcttgct gttgattagc gcgcttatta gccttctagt
     3541 agtacacagg acgaatagaa agacagtgat aagagtagat gaagaaataa aagaacaatt
     3601 gacaacaaaa taaatttgat ggaggtcgtg taaaatgaca aatgaaaaac ataaagatag
     3661 atggctttgg tatcctggag actttgagat ccgccatgga ctgctacaaa atttccaacg
     3721 ggaagaacga ggttttgact ggccagcgta ctggtacatg gatgattgcc atcggaacgt
     3781 caagtttaag agatattact ttcttgatca accttccatg tttaaagtga cgatccaagg
     3841 agttgggtat gtagaaatta atggacaaaa acatccatgt ggaaaatggc tgacttgtcc
     3901 agctggtaaa gcaaaaattc gtatctttgt aggacataca tcagggctac cagcgatgtt
     3961 tatcgaggga gacgaagtaa aatcagatat tggctggaca gcaagtaatt ttattgaaga
     4021 atatccagct ggctggagtc cgctttatgt cgatatcgca aaagatccaa atcaaatcta
     4081 ttatcaaaaa gaatgcgtcc atccagtcac tgaaacggag attggcggag gaatcttatt
     4141 cgattttggg cgagcagtga atggctgtgt agaactaata tctctggatg accatatcga
     4201 atctatcact ctatgttatg gagagtccga tgtagaagct atggatatcg attattgcta
     4261 ctataagcaa ataggaatga caagaaaaat gaaagctcgt aaacgcgctt ttcgctattt
     4321 gtttatacca gaggtttcta ctggaatggt tcaacttcaa gtttttcatg aatttttatc
     4381 tctttcgaaa caagctgctt tttcaagcga ttcttcttta ctgaatcaga tctggtcagt
     4441 atcagaagaa gccttcaagt tatgtagtga tttgtttttt atcgacggta tcaaacgaga
     4501 tcgctggatt tggtctggag atgcgtatca atcattcatg atcaatcaat atcttttttt
     4561 tgacgaggaa atcaataaac gaacgatatt agcattaaga ggacaaaatg aaatcaagca
     4621 gcatatgaat actatcgttg actattcgat cttgtggttg attggtatcg aaaaccacta
     4681 tatgatgacg aaagactatg aatttttacg catcgtctac cctaagatgc aatcattaat
     4741 gagatacttg atggagcaaa cgaatgaatt agggtttatt tatgggagag caaaagattg
     4801 gatatttatt gattggtctg aaatggataa agaaggaacg cttgctgcag aacaagtctt
     4861 attactgaaa gcctatcatt ccatgatcac ttgcggagat atcttagctg aaacatatgg
     4921 agagaacgaa aattatcata ctcatctcta tcaagaaaaa tatcaactat tgaaacaaaa
     4981 cctatttgac tacttttggg atgaagaact tggcgctttt atcgattgct atgaatctgg
     5041 gagaagaaat gtgacgcgtc atgcgaatat tttcgctgtt ttattcgatt ttgctaacaa
     5101 agaccaacag caatcgatct taaagaatgt cttattgaat gacactatca cacagattac
     5161 tactccatat ttcaaattct atgaacaaga tgctttatgc aaactcggac agacgaatat
     5221 cgtctaccaa acgattcttg attattgggg agggatgtta tctcatgatg cggtaacttt
     5281 ctgggaagaa tacgacccta ctcaaacagg aaatgaaaag tacagtatgt atggagatcc
     5341 ttatggaaaa agcttatgcc atgcatgggg agcaagccca atctatcttt taggacgcta
     5401 ttttatcggt ttgtatccaa caaagccagg ttatgaagaa tttatgatac aacctaatct
     5461 agattcattt gaaacactga attgtacgct acctatcaaa gatggaaaga tttcgcttga
     5521 aaaaacaaaa gataagatcg tgattacttc tgatagaaca ggcggaacag tgaaaatgaa
     5581 tggtaaaagt tatcctcttc tagcaaaaca gccacttgtc ttaacaagct attcaaaaat
     5641 aaatgcataa aacaaaaaac tgcttgcgac atatcagaag actttaaatc ttcttctgat
     5701 attgtccaag caatttttat tttgaagttt gcttaattct gttctttaga aacaatgttt
     5761 gttctttttc cggcaaaaag aaagctagat aatacgacaa gtaaaaagaa agcgatgatt
     5821 ccaactgtca aggctatttg accgtattct cctagtttcc caagtacaag tcctggtact
     5881 agataatcag tatcggcaaa tgtagaagaa gcgattttaa gatctcctaa tactggaagg
     5941 ataaataatg gcagccaact aatcaataag ctattgaaag cagaacctaa taccgcccct
     6001 cttctaccgc cagtcgagtt gccgaagaca ccagaagcag ctccgcagaa gaaatgtcct
     6061 actaccccag gaatgataac tgtagtttgc aagcctatca tagcaaacat ggatagtgtt
     6121 cctacaaaga agctaacaaa aaagccaatt aatacagcat taggtgcata tggaaaaacg
     6181 ataggaacat ctaatgctgg tttagaatcg ggtacgagtt ttttagcaat cccttggaat
     6241 gctggaacga tttctgccaa caccattcga accccttgaa ggacgataac aaacccagct
     6301 gtgaatgttc cagcttggat cagtgcaaag attacagcgt tgtctccatt gcttagttct
     6361 ctttcaacaa atccaggacc agctaacaaa gcaagtgcga catatacgat cgacatcaag
     6421 agcatgatcg atacagtaga atctcgcaaa aagctgaaac ttttaggaat ctttatgtcc
     6481 tctgtagaac ggtctttgtt tccaaaaaac tttccgatcc atccactaga agcataaccg
     6541 atatttccgg tatgccctaa cgcaacttga ttgtttccag tgatgttctt cataaacggt
     6601 tgacaaagag caggcgtaat agttaatagt gttccttcga agaccccgcc taataagatc
     6661 atccacaaat ttgcagtaaa ccccgcgctt actaaaatga ctgctgttag acaagaaaca
     6721 taaagcattg cttgacctgt aagaaaaatg aatttatatc ttgtgaatct agcaagtaaa
     6781 atattcacga tcatccctac tagcatgatc aaagcagtag gtgtaccgta ttgttgcaaa
     6841 gcaatcgcca ctactgcttc attgctagga actactcctt gagtatgcag agctgtttgg
     6901 aacatcgaag caaaaggggt tagtgaatta ctgattaaac cagcaccgcc gatcagtact
     6961 aggaatccga caaatgtttt gatagttcct tggatcactt ctgaaaattt cttcttctga
     7021 agaacgagac caagcagtgc aatcaaagaa acgaggatgg agggctgact gacgattgac
     7081 acaaacaaat tcaacaattt atccattgat agttcctccc ttgaatcatt tttattcttt
     7141 tgacaagatc ttcgttactt tttcttttac ttcttcgctg tcaatgatcg attgcaaagg
     7201 tacgagcttt tcatcaggta tagaagttag cgtatctttc aaagaatgct caatgaagaa
     7261 ataatcagcc atatcgctag atacacttcc aacatccgta tgacttactt caaactcgtc
     7321 cacatctgct ccaagatctg caagtgcttg tttgatgttc atctctacca taaaacttgt
     7381 tcctaatcca ctttgacata ctgcaaaaat tttcatatca ttcgtctcct tttcttatga
     7441 tcgctaatat ttcttctttt gttgatgcgt gtaataaatc ttgaagattt tgtttattag
     7501 aaagtagctt tgttaaattt gccaaagccc gaagatgtgc ctcattatcg atagcagcta
     7561 aacagatgaa caaactaatg ggatgggctg gattatcaag cagatccaca ggttgttgaa
     7621 gtttcagcaa agacatccct acttcgttta cgccatcttc tggacgagca tgaggtaatg
     7681 cgatgtgatc gccaatatgg ataaacgcac catactgctc cacgcgctcg atcatagctt
     7741 tgatataact ttcttctatt ttattttctg ataatagcgg ctgtgcggca aatgtgattg
     7801 cttcttgcca gttttttggt tgatccgata attggatcat ttcttctgtt aataattcag
     7861 atagcatagg acgttgatcc tccttctttt ctaaaagttt gttcatttta cgatttaata
     7921 cttgatagag cttttcttcg tctacgcctt cttttaattc gatataaggc agtagcgccg
     7981 acaacaattc ttgggcagaa ggcattgaaa atcctggtat cagccaatct ctttgtactt
     8041 gattgatcag ctgatttttt tccaagtcag tcatgattgg atgcagaaca tagagagact
     8101 ttttcttatc agtaagtgtg agaggtacag tagaaaaaat aatatcgtaa gaagtttcgt
     8161 caacctcgtg taagcggtca gctgaattag tctctgtaaa aaagatcatc gggaataatt
     8221 gctgaagttc actttttaag atcaatgaag agctaatgcc acttggacag acgatcacgg
     8281 ctcttatttt tctatttctt tcttctgcgt ccgcttttct gatctctccg ccaaatagta
     8341 tagtgaaaaa accaatctct tcttcaggga tgctcttccc tattcgtctt tctaacggaa
     8401 gtaatgcttt tcttgtcatt tcgtagatgg aagcatattg atgcttaatt tgatcgatca
     8461 tcacattagg taaataaaaa gaataggaaa tacgaaaata ggcagggacc aagtgataaa
     8521 agacattcat caataattca cgaaacgaag caaattctac ggccatcagg aattccatgc
     8581 gatggatgac ttctgaagaa cattccagca aaaaatcaag tgcaggctcc tggacatctc
     8641 cttcaacgat cgatgctaag caaagagaga aaaatataat ctccgaattt tgatgttcgg
     8701 aatagccaag gccagtcaat acttgtcgac taatgtcagt gactgcaaga gaagccaaca
     8761 gttcagcatc ttttaccagt tccatggatg tttttccaat tcgaatggtt gtcaaactca
     8821 aataataagc taattcatcc attcgatttg gtgcgaatcg aagatctgat agtgaaatcg
     8881 ttttatctag caaatctcta actttggcgt aataatcaat ggaaagggtt gcggttaaat
     8941 acgtgagccc aaatcttcca gcaagtgact gcatcaaacg agcagtgcga ttttttgctt
     9001 ctcttcttaa tattgcttct gaccctagca gcctaaaacc agttttccta gtatacgaca
     9061 gctgacaatc ttccacttct aattcatttc ttatcttttt gatatctgac aggactgtcc
     9121 ctttagaaac ctgaagaaaa tcttgaaaat gaaaaacaga aacagacgtt gcagtcgaat
     9181 aaaccatcaa ataaagaaga tctcttcgct cttgttcggt atacacagct tcaaatcgag
     9241 taatatccaa aaatttttct gcccagtcga ttgctcggaa tttctttata cggatacggg
     9301 tatcatccac atattcggca aaaggagtta attgtttgat cgcctcgctt gtatttactt
     9361 cgatgatctc tgctgctttt tctagactga tttctcttgg tgttttcagg agataactaa
     9421 ttttgtagtt cacagcgctg ttcacctcct acttatattc tagtatggta gcgcttttaa
     9481 atcgaggggt gtttggtaca acttacagtt ggattttttt tacgactgta ccgaacgatt
     9541 tctatcgaat ggatacagaa aaaaagcagg tgaccaaatg tctcctgctt ttatgaattg
     9601 atttcgtccg atcaatgatg atcatgttta ttttttgagg tcagttcttc aaatttttct
     9661 ttgacatctt caaccgcttt ttcgatatct ttttcaaagt gattgggctc attaggcttt
     9721 gtttcctctt ctacagaatt tgaaccagat aaagctgttt ctttattttc tgtttcacca
     9781 gagtttgtgc cagctaatga ttcttctttg tcttctgttt caccagaatt agttcctgct
     9841 aaagccactt cttcttctgt tttaggggcc attgaacctc ttaatcgttt gtgtttttca
     9901 ccgtcataat ttgtcatgtt ggttcctcct agtagttcta tactttttca tcgtacaata
     9961 agacagttta tgcgtcaaaa aacaaacccg attcttacaa atatgtcata tttttccttc
    10021 tgattcttga atgcttttga ttccttcttc caaaattttc atttgtaaga ttgtttcttc
    10081 atctttgatg actttttctg cgccattaac taatgtctcg taaatccctt cgtagacacg
    10141 gctgtaatca ccaacctcgg aaattacttt ttcttcatga taactccctt gttcatctat
    10201 ataggtcaat acaccataat gttcgggcaa atcgatacca aagtcttcat gatcaggcat
    10261 gtaaaacatt ttcagatgtt cttcttgtcg atcttttgtt tgtttcacga acatcccttt
    10321 ttttccgtaa acgacgaaac ttggacgttc tttgatgcgg aaataactgg attttatcga
    10381 aattttcata tttccataga aaaaatccag atcgaagtaa tcattcattc gtcctttccc
    10441 taaaagttga cgtacatcat agctgatatc atctggttcg ccgaaataag aaagaacttg
    10501 atctactgta tgacaagcat gtccatataa atagctgttc gcagtagaaa attcatgcgt
    10561 attttctggt acttccggtc tgaaataatc ataatgcatt tctacttcta aaatatcccc
    10621 taattttccg ctttcgatta ctttttggac tgtcaagaag tcagaatcga accgtctgtt
    10681 ttggtaacat tggataaata gacctttttc ttttgctaat gcaaataact cttctgcttc
    10741 ctttgacgtt tctgcaaaag gtttctctac tagtacattc ttaccatgtt cgagtgccat
    10801 ttttccatat tcataatgaa attgactagg tgtagtgatg acaactaatt ggatctcagg
    10861 atcatcccaa agatcagaca gctgatcagt atattcgatg tcttcgaatg ttttccacac
    10921 tggttcagat ggggaaaaaa tttttttgat attgattttg ttcgtaagtt ttcttgaaaa
    10981 aggcaaatga tagcgatttg tactctttcc attgccaaga taagcgattg taagcatgag
    11041 tggtgcctcc ttttattgat gttttcagta tatcctagaa aagcgcagaa agtatttata
    11101 atagctattc tggtaaaaaa gttcagaaaa acacttgtta tcattcggtt tggtgaaatt
    11161 aatgttcaaa gtaaggataa aaatcaaaaa gatagtagcg gaaccaagtg tttatcagta
    11221 agatagaatt tggaggtcag atcatgctta ttgaagaaaa actaacaaaa caagagttgt
    11281 ttaccacgac agagaaacgg attgtcgatt acatccgtag gaatatcgaa gctgcagtct
    11341 acatgacgat tgaagaatta gcaaaagcta cgtatacttc ccattcagct atcattcgtt
    11401 tatgcaaaaa aatgggattc agcggattta aagaattccg tttcgagctg gggcgggaag
    11461 ttcatcaatt gattgctcag ttcaatcaaa cgattgatcc taattttcct ttttcaagtg
    11521 aagatagcac tcagcaaatt gcaaagaaaa tggcggattt atcgatccag tcaataaaaa
    11581 aagcacaatt gcagattgaa catgaagatt tagatcaaat cggtaaagta ttgaacaagg
    11641 cacagcgaat ctttcttttt gcaaaaggtg attctcaaat cacggcaagg aaattccaga
    11701 ataaaatggt caagttgaat aagtttttga tcatggctga agaatacagt gattcttctt
    11761 ggaatgcagc caatttgact agcgaggatt gcgccatttt tattagctac agcggtcgca
    11821 tccatcatta tgaacgaata atgacctatt tgaagcatgt agaggcccct accttgctga
    11881 ttaccggaaa tcagcattca gaaatggcca aacaggctga tatgtgtctt gttatatcgc
    11941 aggaggaata tgattttgct aaagtagcga ctttctcctc tcaaattgcc ttcgattacg
    12001 tattaaatac gttattctca gtgatatatt ctcaaaattt tgaagagaat atccgcaact
    12061 tgaaaaacaa acaagaattg atgcaaaagg gccttcttaa agaatatcgg aaaacttaag
    12121 aaaagatcct catttagtaa taagaaagat agtgtttgat aggctctctt tgatatcatc
    12181 ataaaaacag gctgaagttt tctttttcaa acctcagcct gtttgtttac ggaacgattc
    12241 gaatagatat attatagaaa tgaggaatag ctaacttctg tttcttattt tgaacgattg
    12301 acgaaaaaga tatataagaa ggaggaaaat cagtatacat gcagctaaca atccatacag
    12361 gaaaaatcgt ttgttctcgg atactttctg tacttctttt tgaacttctt ttgcatgata
    12421 aggaacccgg tgccctctga caagtaagcg atgactgttg accatataag gtgtacaagt
    12481 taggagtgtc acgaaatcct gccctttttc gattctcaga tcttttgtat cagtcggttc
    12541 taccgtcttg atctgatcga cttgataagc gagtgtccgt ccattgattt cgatatagaa
    12601 agaatcacct aaagtcagtt tcggtaagtc tgtgaacaac tttgcttgag gcagtcctcg
    12661 atggctggaa ataacggcat gtgtattttc tccccccgta ggataagacg ttccttccaa
    12721 taatgaggca cctttttcta gcagcacagt attcgtttca tcaaaaattg gcaaatggac
    12781 attgatctca gggatcgtca aaataccgat cgtatgtttc tcaaaatagg ttctatcctt
    12841 tttcgcaggt tcttttttct tagtaaaggg atctgcacct ggatttccac cttcttttgc
    12901 taattgctgg tttttctttg tcatgttttc ttggatttta gccatgactt cttcattttc
    12961 tttgaccgct tgttgctgat aatgagagat gatctgctga tccagataat tattcagcgc
    13021 gtcgctaaca aatggataag ctagtatccc tattcctgag agaagcaaga aaagcatcag
    13081 cagatctaga agtttccttt tctttggttt catttgtgtt ctccttgtga tgactgtttg
    13141 ataataaaag aaaaaggagc atttgcgtaa tccgctgctc ctttttccaa ttcttgcaaa
    13201 aagactaaat ctgactgtgc ttgcgtctag caaagtacag tccagcaatc agtagaagga
    13261 ctactcctgc accgatatac acatagattc ccttaccgcc tgttgaagga agtgtacctt
    13321 tgtgtttatt tggtattttt tcaggagaaa tcaactgtcc tgctgttaca taagattgtt
    13381 catcgatagt aaatgctaca cggtttgtta atggaacata tttttctggc gctttcgttt
    13441 cttccagata gtacgtgcca tattttagac ctgtcacatc gattaaaccg ttacttgtgg
    13501 ttgtaaaaac cgttgctgat tctttcgccg atacccagct gacggctttt gtggatgggt
    13561 cgatcgataa atatttcgct gtgtcactat cttgatcacg aacgacgaat tctgctccag
    13621 caagtgtttg gtctgatgta acgtcaccat ctactttaac aaatcgtttg cccccagtaa
    13681 cgacatcgac tgacggtggt gtttgatcat ttgtatgccc gttatcgaca tttgcttggt
    13741 tagaaaatcc tttggttgga tctgcttttt cgttcaaatg catatagtaa acgaatttca
    13801 atgtaccgcc aggagttaat gaaggaatat aattcggatc aactgaaacc gtgaatccgt
    13861 ctgtttgctc agtgacagaa taatttactg ggtcgatttc tttatttcca tcatataagg
    13921 cataagcata cgttcctgaa gaatcattat caaatgttaa agcagcgtca tgagtatcga
    13981 taagtttgaa tgttgtgtac ttgttttgcg ttccttcttt atcagcgata cctaatggga
    14041 tattgacaga aatctcatat tgaatttttt caccgattgc gacatctttt ccattcaatt
    14101 gaggtgttgt tttatcaact ttagacgtat catttttgat ggtcttttct actggtgttt
    14161 ggctatttgc caggatctca aaaggcgtga ttgtttgctt ttcaatcatt tccgcattat
    14221 caggagcttt tacttcttct aaattatatt ttccaacttc taaatgatta acgatcaact
    14281 ggcctttttc aatagatgcc tcggcaaagt cattgttgcc gatgtcataa gaatgaccag
    14341 taatgaaatg tttggctttg gtttgatcag ttgtccaagt gtacaatcca tctgtgacac
    14401 tttggatgta ttttttgacg cttggtgttc cttcttcttt agaaataata aattctgctc
    14461 catttagtgc ttcgttttcg gcagtaccga tttttgtaac tttcaacgtt ccatcattac
    14521 agactgtatt tttagggtag agatggatag tatctagttc ttctgtcccg tatttataag
    14581 agccatctgc ttgtttgatc atctcatata cagggaaagc taaaaccatg tttgcggcag
    14641 ctgacactcc gtcttttggt tcttctttga tcgtgtagac tgcatctttc ccattttgtt
    14701 ttttaggtaa agataaagtg acatttccat cagcatctgt cgttcctgaa gcaactggtg
    14761 taccaggagt caaagactgg actgcttgtt ttgctgcatc cacggacgct cctttatctc
    14821 gttgcgcata aaattcttga gtgacgttat aaactgaaaa tgaaatatcg gctaatcctt
    14881 ggtattgatc gaattcgctc atttctttcc cgctgttttg gattaaagga tcgggtaaat
    14941 cagtcatttt ctttttatga aggatgactt gagctgctgt ctcctctgca aacacttttt
    15001 ttgcgccgaa gctgtttgtg agtaatggta aaataaggaa aaggactcct aacataacgt
    15061 ttattttttt atgatttttc attttttcac tcctgttctt tttttgataa acaaccagat
    15121 tgcaaagctt gcgaatacca taccaacaac gcctaaaagt gtatttttcg tttcgtttgt
    15181 ttttggaaga aatggacggc tattttcttt tttattgact actttcaata tactgtccgc
    15241 ctctttatcc tcagaaagaa aagttgaaat ggtaaacgga atttctgttt cacttaaaat
    15301 ataaccagaa ggtgcttcga tctcttttaa tcgataactt cctgttttca acccggaaat
    15361 tgaaaaagcg ccatttttat cggaaatcag gtgaagcgca tctgattcgt tctttgtcca
    15421 cttgtatccg ttggctgttt gctggaggta atttcctgct ttgtttttaa taaggaatac
    15481 tgcgccagct aatcgtttat ctggtttatt tgtctcgatt ttgacaaatt gcttactccc
    15541 tgtatataca gagactttct ttgtactgac accaaaacca aaatccagtg ttccgttgtt
    15601 gttgatttcc ttgttcgcct gtgctgtact gcttagctgc atctgataag aaatggtcag
    15661 tttttttcca gcatattttt ccaacgaagg gatcgaaaat aaaacatcaa atccgtgagg
    15721 tttcttgtgt atgacaaagc cttctgtcag cttttctcca ttcgatgaaa tcgttaaact
    15781 attaggtaaa aacgttaatg cttcatccgc actatctgac aattcgaact tttgatagtc
    15841 aaggatattt acaggaattg tcgtatgcaa ggaataagtg attttttcac caatcgtaaa
    15901 atcatttcta ggctcttcta ataccttctc aaaaggtgga tcagggtagt cgttttcttc
    15961 atttttagga tacaaatgga tagttgaaag tttttgattg ttttgtccat gtacaggaag
    16021 aacaactacc atattctctg ccttttcttt gacgacttct ggtactttgg attcaatgaa
    16081 aagatacgct ttatctcttt tttcttgatc tttagcggcc agagaaaaag aagcgatacc
    16141 atcttcgttg ttaagagttg tagttgttgc ttctgcggta aacataccgg aagacgcacc
    16201 gttttttgcg atctctgctt gtgcttcttc taccgttttg cccttttccc gtagatggta
    16261 aaaagaatct gtgacatcat aaacttggaa tgtgacacca tttaatcctc gatacgtttg
    16321 taataaagct ttttcttgtc cgtcatttgg atgatttttc ggcagttgcc cgtttgggaa
    16381 aagcaatttg tgtaaagtaa tctgaaccat ttctgtttct gtttctgttg ctacctgagt
    16441 aaaagctggt ttaaatagta acaagaagag acacatacta agccaaccaa gttttttcat
    16501 atttagctgc cccctctttc ttttttcaaa aaaagatacc aaatagaaaa cgcacaacca
    16561 atcatcccta ctagatagat tcctaaacgg ccaattcctc ccgtttcagg taatggtact
    16621 tttgcctgat tcgtgatgtc taaagaaatc tggttgtttt tggctcctgg tgacagaaca
    16681 gattcatgat cttgtccatc cacttgaatt gacccatctt cgtgtataac tatagtaact
    16741 ggctctttta gaccttggta tccttctggt gtaaaagttt cggtcagcgt ataagttcca
    16801 ggttttaaat tttcgaatag aaaggtatct gtttcttgtc catcttttgg cgattctaag
    16861 tccatttctg gtccctgcag tctgaatttt gctcctttta atgtctgtcc gttgtcttct
    16921 tttttgtgta ccgttagatc aaacgctttt agtcgatttt gatgagtgag cgtccataca
    16981 ttatttgtca tctcggtatt ttttcccgtg taaatgattt ttccatattt atcgatttgg
    17041 aattcataat ttcccggcga agtatcatat ccggcaggac cagctgtttc aacgactcga
    17101 tatttaccag gtgtgagtga atcaaaactg gcaagacctt tttcattcgt tgtttggctg
    17161 tcaattggct gataagtacc atttgcttct tttttctgta gggaaaatgc tgctccttta
    17221 agatttatct ctttgtccgt cccttgcatc gcatacttac gtactgctac aggaacttca
    17281 acaaaaggat tttcaatagt caactggatc gtatcatcgg aagtagtgac tgtttttcca
    17341 tcaatggtta ccgtaccatc agaagcgatc ttgatttccc aagtagtctt tttgcttaat
    17401 ccatgccctt ctggagcttt tgtttctgtc agcgtatagg tcatttcttt ttgcaatttg
    17461 acattttctg gaagagaata ggttccgtcg ccatgatctg ttagtaaagt atcaatagaa
    17521 tctcctgtta atttgaaaac agcgccaata agatcctttt cacctgtaga ggaatttttc
    17581 gttattttca agtttaacgg tgtgaattgt ttagtatttt tccatgtcat tgcatctatt
    17641 tgttgagaat cgtatcctgg tacaggtaat tctttgattg tttggtaact gaatgcttga
    17701 ccttgattat tgtattgagg tagtgataat atctcttgat aactttctcc gctatttgca
    17761 gataatttgt caatgtctgc acgttcccac gtatttgttg tatctttagc tggtttaatg
    17821 attcgaatat atccgttttt ccaagctgca gcatttgtcg tatgttcccg ttgaatctca
    17881 aaagtaactt gatctggacg atcagctaga ttgttgtcaa attcttccca taactttttg
    17941 atgtgaagac tgactccggg agctttagca gatggtatac cgaattcagc taattcattg
    18001 gtatcaatgt ttggctggaa agttgtcctg ccgttcattt gataccagaa atttggatgg
    18061 aagtcctcat tttctgtttg gattctcact tggtaatgga tttggatttc ttggtctttt
    18121 cctaaataga tctgattgct cttgatggta tttccttcta tggaaataga tggagtgacc
    18181 gttgtaggac ttgtccccac actcttgact gaaagtgtac caggctgata aatgaatggt
    18241 tctgcgattg gatcatttat ttgtccattg cttacagttg ctgagatctg tacagctttt
    18301 ttggctaggt attcagagat atctgttgca tgatcagctg attcatagta aagatcgcct
    18361 ttttcatctg atgaaaccat ttgtcgcata cgagactcta cttctgcttt tgagagacca
    18421 gctgccggat cgctttgaag ttggatgcca agaccatgta tttcgattcc tcgttctttg
    18481 agtgccatcg cttctccgat ggttgcgata aacgtactgt cgattcttct ggatagattg
    18541 ttttggtcag gtgcatcata gattcttgag attagagaag tatttcccgg ccgatcttgc
    18601 gtattagaaa actgagttcc gtaataattg ctgcttgatt gtgcgtgtac ccgctgtact
    18661 ttataggaaa atgttggtac accatccgtc agcaaaacga tcactttttt atgtccattt
    18721 ggaacggata gcatgcttcc tgcatctctt agtgcttttt gtgtaaaagt accaccattt
    18781 gtccgtgaag gtgtaatgga ttttacttga tttttcactg aatcaaatga acccatctgt
    18841 actgcaccgt tactgtagct atatccttcg cttgagtagc cgacatatcc catattgatt
    18901 ttgtctgtga taccgctatc tgctaaagta tcgacaaaac gatcgacacc aatctttact
    18961 tcaccgatcc gattattgtc gttcatactt cctgaccagt ctacgaccaa tacgagatca
    19021 agaggcgtga tatctttttg tacatttcca cgagcattca aataaacatc aaacaatccg
    19081 ggtgtacttg tttcctttgc atactttcgt atcgcatagt ctgcttgatt accggttcct
    19141 ccatattcga tatacgaatg ggtccgatca ttaggatctc cattccaaga atttatacca
    19201 tcccattgat ttgttccgtt tttattgcct tgatgatcaa gaacatttgt atttcctgta
    19261 ggttgccaac tggctttagg ataagtgccc tgatcatctg ttgtatattc tgtaggaatc
    19321 gtttgattag atggcacact cacagctggc aaggataatg taggcaatgt aaatgggctg
    19381 acagaatttt cgttttcttt tgatgattct tcttctattt cgtctggctt tggtactgac
    19441 acatctgtat ctttcttatt ttctgtctca tcaataatgt catattcttt attatcgacg
    19501 atcagcttgt ttgcctggct gtggtaaagt gctggatcga ttggaaaact cagatcgata
    19561 gtttgtttag tgtttgatga gatttccaac gtaattttcc cgctatttac ctgataagca
    19621 tagccatctc cttcttttct ttccacatca cttaagtaat tttcgactag accaatttct
    19681 ttttctatct tttcagcact ggtgttattc gcatttagct tgatgttcag tgcttttttt
    19741 ccagactgat ccgaaatgac ttcagctgaa attgtttcta aaactgttgg atcatctgta
    19801 gtgtctgcta ttgcagataa tggcacaaag tttgataaac tcaatattat cagcatgaaa
    19861 ataactttca gtttcttccc tgtggtcgtc atttttcccc tcctttttct ttttttctgt
    19921 tttgacgtga atagtgaaaa ttattaaaat aaacaggcgt tacataattt tatttatttt
    19981 aatatgataa ttattatacg aagggcgcta aagggaatca caccaatttt cataccaatt
    20041 ggtttcattt atgaagttga ttccttcgat taatgagcgt ttcattgaaa gaaaagggtt
    20101 gagggagaaa aacatatgat caggttgttg gaatgcaact atagaaagat ggaaaaaatc
    20161 attcgaattc tgtccatttc tgacaagtgg atgactaaaa aagaacttgc caaacgaatt
    20221 ggtagttcag aatctacatt tgtaaggtat atcgaagaga tcaaaatccg ctggggaacg
    20281 gatgtaacga ttcaatcttc acaaaaattg gggtatcgtc ttgaacattt gaatgtacca
    20341 gcttatttaa atgttctgat ggaaatgtcg caaagttcta caaacagtcg tttattaaat
    20401 gagattattg tcaatccggg taaaacgatt gagtattatt gtgaaaaaat ttccatcagc
    20461 cggtcttctt ttacacgtaa gttgaaacaa tgcaatcaaa ttttagaaag ctatttatta
    20521 aaagtgatcg ttgaccaagg ttttcagtta actagcctta aaagtgagct ccagttgcgt
    20581 atctttgtta cctttttctt tttaagttac tacggaagac gcacgttgcc atttccaatc
    20641 gaaaaaaaga agatcaaaga aattatggtc aagaataatt gtcagatgaa tttgtttcag
    20701 gaagaaagcc catatgagca atctttcttt attatgtatt ttgttgtgca tctcatgaga
    20761 gaatcccaag gaactccatt tgaatggaaa gctggagact cttatgtaca gttgataaat
    20821 atcgataaaa aagacttctt aatcttgaaa aatctcttag gaaaattgaa gtacagcagc
    20881 tatataaaag tagtgaacta ttttgtcaat acgatttttt cagaattttc ggagaaagaa
    20941 aaaaatgaaa tcagtgagca gatacgaaaa aatatgtcct actgttcgtt ttcatccgct
    21001 gattgcaaag aaaaaaacga agaatatgtt gttcaattat taacgcaaat ttattttttc
    21061 tcccgtattt tcccatacga tacgactcat ttgacgatcc gtgcaactta ctttgcacag
    21121 cagatgcaac accgccagat acgtctatat gctggatttc gtgcagaact tcaattttat
    21181 tccactctac tgaatcagaa tctgatttat tatttatcta gctatatctt ttggatgctt
    21241 atcggtaatc ctgaaattca ccattatact tctgataaaa aaatactgat tatcagcgac
    21301 ttaagtcttc gacatagtca atatattgaa gaatatatca gtgatattct tgctgtgcat
    21361 aaaattcact ctgagacaac tgctattact gaagatcagc tgtcgaaata caatcttctc
    21421 gagtatgatc tgatcgttac gaatcaaccc attttgaatg cccatgttcc tcatatattg
    21481 attgatgata gtgtgtcatt cgcaaatgaa gaagaactca tccgcttatt cgagttatga
    21541 ataatcaaaa aacagctatg tataaaccac cctgataaaa atttcaggac aaatacacat
    21601 agctgtttct ttttattgta ttccttcagc aatccaacga gatttatagc gaaagtcttt
    21661 taacaactct tcttttgtcc aattatttgc aatgagatcc gtcaaagtgt tcattacgag
    21721 ggtcatggaa aagtatagga aactaactgc ttcttccgac gattgaatgg agaaatgatt
    21781 gattcttaat aattccagcc atttttcgtt cattggagaa tcagccgata gttctctacg
    21841 tttggttgtc tgctgatcat ttcgctgggt taacaagtat aacctttgcc aatagatact
    21901 ttcgtgatct aagtcgcagc accactctaa atatttttcg atccccaaaa agaaatgttg
    21961 tttgttttcg ctaatgaatt ttaagatatc ttgatggact atgtgagaac tgcttttgat
    22021 cgtatacaaa taagcatctt ccaaatcttg aaaatattta taaaaagcac cgcgcgacat
    22081 ccccatcttg tcaacgattt ctgagacttt gacttggctg atgtgctgtg agcaaaatat
    22141 ctccagtaat aggtcatcga ttctctgttt ctttgcgttc gataaattga aaaaagtttc
    22201 ttttggcatc cacatctctc ctttatctct gattatacgt ggatagccct tcattgtcat
    22261 gaaggattca ttccttcttt ttctcttctt ctgtcaagta cccatcttcc attcgataaa
    22321 cgcgatcact ccattgaatc atacggacat catgagtaac cataatagtc gctttttgtt
    22381 tctcatgagc ttctttaact agtaatttga cgacttcata tgcatgatct gtatccaaac
    22441 tagctgttgg ttcgtctgct aagatcaagc ttggctcatt atataaagcg cgagcaattg
    22501 ccactctctg tctttctcca ccagaaaggt ctttaggata actattttta agtgactgga
    22561 tatctaaaga ttgcagcaag gaatcgattt ttgttgaatc ttcttttttt gaagtaactt
    22621 ggtcaactag ttcaaattgt tcctttaccg ttaaaaaagg aatcaaatta gagctttgga
    22681 gaataaatcc tatttcttta aagcgtaaat ctgctcgttt tttttctgga agctcagtta
    22741 gtgagtgacc gttgatcact atttctccgg aagtcggtgt ttgcaatcct cctgaaatgg
    22801 taaggaaagt acttttacct gaaccagaag gaccaatgat gctgacgaat tctccttcct
    22861 ttacataaaa atcaattgct ttcaaagctg agacctttgt atgaccagaa ccaaatgttt
    22921 tggtaacttt tttcatcgtt aatatttttt tcatttttat taacctccta ttgctgtaat
    22981 tgggtctact ttggttatcg tacgtatcga aaacaatccg cctagcattg cgacaatgat
    23041 caaaatccca ctatatagca accattgtga ccaaaaaaca gcaaaaggca tcgcgcttgg
    23101 taaaatcaga ctagtcaaat aagcaaacaa aagagcaagc agaacgccta ccaccccaac
    23161 aataaaggac tgtgctacta aagatttagc gatgaagaaa tttttgatcc cttgtgcttt
    23221 cattactcca aaaatagcag tcttctgaag tgtcatcacg tacatgaaaa taccgaccac
    23281 ggcagcaacc actaagaaca aaaagtatat catcgcattt aaggtcatat tttgggcaga
    23341 atagccagga atactttcaa tgaatgtccc aatagttaat aactgtagac ccttcgatgc
    23401 tttttctttc gaaattttag ctgaatcctt tattacgatc ccattgatcg gctgttcttc
    23461 ttcagaagca aacggctgtt gtccatactt caaagcagtc cacgtatcta gggacgcata
    23521 gatgacggga gaaactgtgt aataagtttc aggaaagata ccgacaatcg tcaaaggatc
    23581 gtcgtaactg ccaattttga tcgtatctcc aattttatag cctgaatcag ccagattttg
    23641 agagatgatg atttcattct ttgttttaaa ttcatgccct ttcgtgagtt ttggtaataa
    23701 aaatgcttct ttcgttgtgc cgaaaactgt aacattttct ttgttttttc ctttgattgc
    23761 tccgctgtac aatccgattg gtgctttctc tcctccttca atgtatttca gatctcctct
    23821 tgttaattgt gaagctgcaa atacttgatt tgcatcctcc gacaaaacaa tctcttgtgc
    23881 gtcccaatca tcgattgctt ttttaaactc ttctgaaagt ccattcgcta gtcctgacaa
    23941 catgaatacg acatacgcaa tcaagaacat aataccgacg atcaagccat atcgtaattt
    24001 agaatagcga atttctttga tagctagaaa catttgtttt tcctccctat gatagattga
    24061 tagtgacaaa atgtcacttg ttgaatagtg taactctaaa gtgacaattt gtcacttgtg
    24121 ttgttttttt cttatcaata aattgtctaa gacatctttc gataaaacct taacatgagt
    24181 cataaaaaat ttatttaagc cttatttatg ctttccatga taaactaaag tgaccaaatc
    24241 aatgaggtgg gaaaatgatt aaactattag tagtagaaga cgataaagta ttatccaata
    24301 atataaaaga aatactcgct gcaatcggcg agataacaca agtttatgat ggtagtgaag
    24361 gactgttcga gttggaaagt ggagtttatg atcttgcaat cttggatttg atgatgccgg
    24421 aaatgaatgg atatgaagtg ttagcaaaaa tacggaaaga aaaaatacag actccagtat
    24481 tgattttgac tgcaaaagat agtttggaag ataagattga aggatttgaa cgaggtgcag
    24541 atgattatct aacaaaacct ttctatcgag aagaactgat catgcgcgtg aaagcattat
    24601 tgaaacgctc gctaggtctc tttgatgaaa atgttctgaa gtataaggat atcagttgtg
    24661 atttaaaaag caatgaagtc ttgtataaag aacaagtttt gcccatacaa ggaaaagagt
    24721 ttgaattgct cgtttatttt ttacaaaata agggaatcat tttgacaaaa gagcagatat
    24781 ttgaccggat ctggggattt gattctgata cgacgattac agttattgaa gtttatatga
    24841 gcaacttacg aaaacatctg cgaagtactg gattggataa agaaatcaaa accttacgta
    24901 atgtcggtta tatactgcaa agggtgtgat gcaatgaatg gagatattac acgaaagcaa
    24961 cgtagtcgct tttttgcgac aaatgtagca gcatttgctt ttatcttttt tcttttggga
    25021 ttcattactt tacagatatt aaaccaatct gcatacagag aaacagatct gtcattagag
    25081 aaaataaatt ttgattctcg aacagtacaa ttagagatac aaagatatca gcaaggcgat
    25141 ccctttttaa acccgactca aacggaacaa aacagaacaa acatggaaca gccattaatg
    25201 gaaccatcaa atgaccgttt taacactcaa gtgatcttgt ggtctaaaaa tggtgagata
    25261 ttgaataaag cagctattgg tgggcgtttc aaccaaatca aagaattaaa aatggatacg
    25321 aaaaatctga ataccatcca aaatattgaa ttaaatgaag acagtggctc atctgccctc
    25381 tctttccgtt cgattacgaa aaaagcggag acgaatcaag atggcgttgc ctacatccaa
    25441 attttagaaa atacaaatca aatcaagaat tcattagcga cttttcgcac gattatgatt
    25501 ttgtgtatga ttgttttttg gattctctct attggtatca gttattactt gtctagtcta
    25561 agcatgcgcc ctattattct ttcttggaga agacaaaaag aatttgttga gaatgcttct
    25621 catgaattgc gcacaccgtt aacgattatt caaaatagtt tggaacatct ttttaccaag
    25681 cctaatcata ctattattga agaatccgaa agcatcgctc aagcattgtc agagacgagg
    25741 cgcctcactg gattgacttc ggatttgctg acaatcgctc ggaatgattc tgatcagcag
    25801 ctgctttcaa agcaaatgat caatacacaa gaatatatcg aacaacttgt taaaccattt
    25861 caagagatgg ccatcataga tggaaaagag tttattctcc ataacgatgc aacattagaa
    25921 gtaccaattg atgaaaaaaa aatccatcaa gttttggtta ttttgctgga taatgcatta
    25981 aaatatacga atccagaaga taagatcatt ttggattcat ctgttaacca taaagaatgg
    26041 ctgattcacg taaagaatac tggcccttct atcagtgaag aagatcaagc aaggattttt
    26101 gatcgatttt atcgggaaga tcaatctcgg gcgaaagaga ctggcggata cgggcttggc
    26161 ttggcaattg caaaacaggt aatcgaacag catcatggac agatttctgt atccaattat
    26221 cttccacaag gagtcgattt taaagtccaa ttgccgttga agcctaaaaa ataatgctta
    26281 tccctaaaaa agggctgtga caaaaatctt gtcacagccc ttttttgttg cgaataaacg
    26341 gtgttccaga agtagcttct tcggaaataa gccgaaattt ctttaagctg accacttctg
    26401 tcacaacctc tttagcttac gcttacgatt ttttattgcg gtcttcaatg tgtacatcac
    26461 cgcttgtgtc aatatccaat tcttctcggc taacatcttc tgtcactttt tcaacatctt
    26521 catgttcttc tttatgaatc ccaacttctt cacgaataac tgtatgtttg cttacatcta
    26581 cttgctcttc tttgattggg atagtaatcg tgtcatcttc caatttttcg tctgttccct
    26641 gtgaagattg atctgttact ggtttgcgtt cgatgacgat ttcttcacgt ttgaccggta
    26701 cttctaccgt ttcagtatcg ttgaccacat gtttgtggat gtcgacttct ccagttgtca
    26761 catcatgtgt atttacatct aatttttctt cttttagttg aattgtatca tcatctagat
    26821 ttggtttgcg atcgatgtct tcagtcttgc ttgtacccat tgtatcagtc tcgccaggta
    26881 cgtccatcgg atcagttcct ggtgtcatat tcaatggatc cgttgtattt tgaaagcctg
    26941 tcgtgccacc tacgtccatt ggatctgttc cagtgccagt ttctaatcca gtccccattg
    27001 tgcttgctgg gctaacagta tcttgtgtat ccattgtatc ttctgttgtt tctggctgac
    27061 gatagccttt tactgtgatg acaaattttc catcagaaat atctcctcta tattgatata
    27121 aaggatcatt ttctggtgta gccgtctcag aatcataggt atcaaaagaa aaagtatctt
    27181 ttattttttc ccataaagaa cggtcttctt caccatccat ccgattttca tctgtttcaa
    27241 catctatacc agacaatccg tcaaatccta gtctagatgc tgcttcatca gtagtataaa
    27301 taacgatatc atctcgttca tacccttcgt tacgcaaccg ttcaattact gataatgctt
    27361 cttccctcgt actatagctt ccgatgaccg ttaaattttt ctcttccatg taaattcctc
    27421 ctagcaaata gatttatgat tggtatattg ttacttataa atttattcta taggagacga
    27481 ttgttttcta caaatattta gattggaatt tgattaaaac aatttaattg aaatttgaaa
    27541 acatttatgt taatgaaagt catattctaa taa
//
LOCUS       DACDEG010000025        27513 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.025, whole genome shotgun sequence.
ACCESSION   DACDEG010000025 DACDEG010000000
VERSION     DACDEG010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.025"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            82..1848
                     /locus_tag="IV142_001190"
     CDS             82..1848
                     /locus_tag="IV142_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP8484859.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            1950..3209
                     /locus_tag="IV142_001191"
     CDS             1950..3209
                     /locus_tag="IV142_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8484860.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3356..4657
                     /locus_tag="IV142_001192"
     CDS             3356..4657
                     /locus_tag="IV142_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8484861.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4660..5511
                     /locus_tag="IV142_001193"
     CDS             4660..5511
                     /locus_tag="IV142_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8484862.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5524..6339
                     /locus_tag="IV142_001194"
     CDS             5524..6339
                     /locus_tag="IV142_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP8484863.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6490..7656
                     /locus_tag="IV142_001195"
     CDS             6490..7656
                     /locus_tag="IV142_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8484864.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            7886..8533
                     /locus_tag="IV142_001196"
     CDS             7886..8533
                     /locus_tag="IV142_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP8484865.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8617..9561
                     /locus_tag="IV142_001197"
     CDS             8617..9561
                     /locus_tag="IV142_001197"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP8484866.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9696..11147
                     /locus_tag="IV142_001198"
     CDS             9696..11147
                     /locus_tag="IV142_001198"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP8484867.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11168..12004
                     /locus_tag="IV142_001199"
     CDS             11168..12004
                     /locus_tag="IV142_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP8484868.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12004..12663
                     /locus_tag="IV142_001200"
     CDS             12004..12663
                     /locus_tag="IV142_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8484869.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            12789..13478
                     /locus_tag="IV142_001201"
     CDS             12789..13478
                     /locus_tag="IV142_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484870.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13484..14440
                     /locus_tag="IV142_001202"
     CDS             13484..14440
                     /locus_tag="IV142_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP8484871.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14484..14996
                     /locus_tag="IV142_001203"
     CDS             14484..14996
                     /locus_tag="IV142_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8484872.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15252..15800)
                     /locus_tag="IV142_001204"
     CDS             complement(15252..15800)
                     /locus_tag="IV142_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8484873.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(15889..17070)
                     /locus_tag="IV142_001205"
     CDS             complement(15889..17070)
                     /locus_tag="IV142_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP8484874.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(17306..18814)
                     /locus_tag="IV142_001206"
     CDS             complement(17306..18814)
                     /locus_tag="IV142_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP8484875.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            18973..19164
                     /locus_tag="IV142_001207"
     CDS             18973..19164
                     /locus_tag="IV142_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484876.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            19367..20284
                     /locus_tag="IV142_001208"
     CDS             19367..20284
                     /locus_tag="IV142_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP8484877.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            20277..21350
                     /locus_tag="IV142_001209"
     CDS             20277..21350
                     /locus_tag="IV142_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484878.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            21498..22016
                     /locus_tag="IV142_001210"
     CDS             21498..22016
                     /locus_tag="IV142_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484879.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            21997..22389
                     /locus_tag="IV142_001211"
     CDS             21997..22389
                     /locus_tag="IV142_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP8484880.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            22465..23400
                     /locus_tag="IV142_001212"
     CDS             22465..23400
                     /locus_tag="IV142_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP8484881.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            23533..24312
                     /locus_tag="IV142_001213"
     CDS             23533..24312
                     /locus_tag="IV142_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP8484882.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            24305..24622
                     /locus_tag="IV142_001214"
     CDS             24305..24622
                     /locus_tag="IV142_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP8484883.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            24866..26476
                     /locus_tag="IV142_001215"
     CDS             24866..26476
                     /locus_tag="IV142_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8484884.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            26633..27481
                     /locus_tag="IV142_001216"
     CDS             26633..27481
                     /locus_tag="IV142_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8484885.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         8893 a         4874 c         5530 g         8216 t
ORIGIN      
        1 ataacatttt tgaaagcgtt gtcttttgtt tctctatcac ttatagtgat gacgtcatat
       61 taaaacacaa ggaggaaatc aatggataca gctgcaattt atcatcgacc ggaaagtgaa
      121 tatgcatttc tatatacgaa tacaagattt cgtattcggc ttcgtactag gaaaaatgat
      181 atcaaaaaag tatatgtgct ttgtggagat ccatatacca taacaactga aaaatggtat
      241 caaaaacaac gaccaatgaa aaaatgtcta agtaccaatg ttcatgatta ttgggaaatt
      301 gaagtgacga gcgaaacaag gcgcttacag tatgcgttcc atgttgttgg ggaagataac
      361 acggactgtt tttacggtga tcaaggaatt tttccttatc gagaaaatgt gattactgaa
      421 ccaaattttt attttcgaat accttatttt catcaaatcg atcgattcac tattccagaa
      481 tgggtaaaag aaacagtatg gtatcaaatt tttcccgaaa gatttgcaaa cggggacaaa
      541 gcaaatgacc ccgaacacac tttaccttgg ggaagtaaag atccagaccg agaagacttc
      601 ttcgggggag atttgcaagg tgtgagggat catttggatt acttgacgga ccttggcgtc
      661 aatggcatct atttttgtcc catatttaaa gccacatcca atcataaata cgatacgatt
      721 gattattatg aggtggatcc cgcttttggc gataaaaaac ttttaaaaaa cttgattgat
      781 gaagcacata aaagagggat acgtatcatg ttcgatgctg tattcaatca tatgggtgtc
      841 cattcaccac agtggcaaga cgttctcaaa aacggagaaa aatcgatcta taaagactgg
      901 ttccacatcc gttttttccc agtagattct tatcagatga ctgacttacc agaaactgct
      961 gaaaatatcc catatgatac ttttgctttt actccattca tgccgaaact aaatacagca
     1021 aatccagaag tgcaaaaata tttgctggat atcgcaactt attggataaa agaattcgat
     1081 attgatggat ggcgtcttga tgttgctaat gaagtcgatc atcatttttg gaaaaaattc
     1141 agagaagcag ttacagaaat caaaccagac atctatatcc ttggagagat ttggcattcc
     1201 tctcaagctt ggctgcaagg ggatgaattc catgcagtga tgaattatgc attcacagat
     1261 tcgattaaag attattttgc aaaaaagaaa atcacagctt cacaaatggt cagtggaatg
     1321 aatcatcagc aaatgcttta cagagatcag gtgaacgaag gaacattcaa cttattagat
     1381 tctcatgata ctgccaggat cttgaccctt tgccaaggta acaaagaatt gatgaagtct
     1441 gtgatggcct ttatgtttct tcaaaaaggg tctccttgta tctattacgg gacagaaata
     1501 gggatgactg gtgaagatga cccggattgc cgcaaatgta tgatatggga gaaggaggaa
     1561 caagatctag agctattcgg ttttataaaa gaacttgttt ctctaaggaa acagttgtct
     1621 aagattattt ctgaaggttc tactcagtgg ctaatagtat atgatcgtga agataagctc
     1681 tatttcacac gggaactaga gggacagatc atctatgttt actttaatca gtcaaaagaa
     1741 ccttgggtag tagaacagga aaatgaggtg attcttagtc aaaattgtca gttgttagaa
     1801 gacgggaaag cagagatacg acaatacgga tttttgatca tgtgttgaaa actgttaccg
     1861 gtaacaacaa gaaagcgttt gcaaatgaag atttgtgccc attataataa gggtgtaaca
     1921 gcacgttgaa tacacaggag gaaatgaaga tgaaaaaaag aagtgtgaaa cagttgggtt
     1981 tagggttaat ctctttagga atcttaggcg gattagcggc ttgtggcaat ggtaactctt
     2041 cagcagaagg ggaagccgat gaaggaaaaa cgttaacagt ttccgttgat tctggatata
     2101 aagattatat aaacgaaata aaaaacactt tcgaaaaaga aaacgatgtg aaaatcaagc
     2161 tagtagaaaa ggacatgttc gatcagttgg aatcattagc gttagatggg cctgctggaa
     2221 agggacctga cgttatgatg gcagcatatg accggatcgg tgcgttagga cagcaaggac
     2281 atctggcgga agtaaaactg ggaaatgaag cagcttacga tgaaacagac aaagcccaag
     2341 tgacttatga tggaaaaatc tacggagaac ctgcagtaat cgagaccctt gttttgtact
     2401 ataacaaaga tttagtcgat acagcaccat caacgtttaa agatttggag aatctatcca
     2461 aagacagtcg ttttgctttt gagtcagaag caggaaaaaa tacaggattt ttagctaaat
     2521 ggactgactt ttattattcc tatggtttaa ttgcaggtta cggtggctat gtatttggtg
     2581 atgatggcac aaatccttca gatatcggtt tgaataatgc aggtgctgta gaaggaatct
     2641 cttatgcaac agattggttc aaaaatgttt ggccaaaggg aatgcaagac ataaaaagtg
     2701 caggagactt cgccagccag caattcatgt ccaataaaac tgcagcaatc attgacggtc
     2761 catggcaagc gcaaacatac aaagaaaaca atatcaatta tggtgtagcc aaaattccta
     2821 ctctaaataa tggacagcct tatcaaccgt ttggcggagg aaaaggatgg gtagtcagca
     2881 attatgctaa aaacaaagac ctctctcaaa aatggcttga ttatgtaacg aatcaggaaa
     2941 atcaagaaaa attctttgaa atggtaaatg aaatccctgc aaaccaacaa gctagggaaa
     3001 cagcaaaggc taaaaacgat gaattaacaa cagctgtgat tgaacaatat gaaacagctc
     3061 aagcgatgcc aaacatccca gaaatgggcg aagtttggac aggtgcagaa aacttgatgt
     3121 tcgatgcagc atcaggttca aaaacaccaa aagaatccgc agatgaagca gtaaaaacaa
     3181 tcagtgaagc catagaacaa aaatactgat taaaatgata accgtccatt tttataaaaa
     3241 atggaacagg tgcttcagat aaaagtggca agagcgagat ggaaaattat cttgctcttg
     3301 ttgccattat cttaaaagca gtttgcggtt tagcaagttt agagggggca ttcagatgca
     3361 gcaagataaa acaagcaata tcaacgaaaa aaaaatcacg ctttgttcac ttattccagg
     3421 gatggggcag tttgtaaatg gccaagcttt taaaggtatc gtttttttcg ccatattcgt
     3481 cctattcatt ggtcagatgc ttctgggcgg atttcatgca ttggtaggat tggttacttt
     3541 gggaagtata ccaatggaag atcattcatt atttattttg attgaaggca ctttgcaatt
     3601 catcattaca atcattttct tgatttttta cggtttgaat cttttagacg cttatcgagt
     3661 agcgaagatg cgtcaagagg gcaaaaaaat caatcggacg atgaaggaag tcatacataa
     3721 tataggtgat gaaggttttc cttatctatt gacacttccc gcttatcttc tcatgatttt
     3781 tacgatcatt ttcccagtcc tagttacatt gttcactgca tttactaatt atgacttcaa
     3841 tcatattcca ccagccagtt taattgattg ggtaggggct aaaaactttt ttagtatctt
     3901 ctttttaagc tcatatcgta atactttttt tgcagtcttt tcgtggaccg tgatttggac
     3961 tttatgtgca acaactttgc aaatcaccct tggtattctc acagcggttg ttgctcacca
     4021 gaaatttatt aaattcaaac gtattttcgg tatcattttc ttattaccat gggcggtccc
     4081 agcttttatt actatcatga gtttttcgaa tatattcaac gatagcgtag gtgccatcaa
     4141 tactcaagtg attcctttca taaaccagct tccttttgta gatatcagtg caatctcttg
     4201 gaaaacggac ccgaactgga cgaaagtagc aattatctta gtgcagggct ggttaggatt
     4261 tccctatatc tatgtgatgg ttacaggaat cttacaatcg atttcagaag acttatatga
     4321 agcagcaaaa atcgacggag caaatgcatg gcagcgcttt tcaaacatca cgcttcctgc
     4381 tgtcttccta gtggcagcgc caacatttat tacacagtat acggggaatt tcaataattt
     4441 ctccatgatc tatctgttta acaatggtgg tcccggaagc gtaggcggag gagctggttc
     4501 tactgatatt cttatttcct ggatctataa acttacgact ggaacttctc cgcagtattc
     4561 tatggcttca gcaatcacgt tgattatctc cgtcatcgtt atctgtgtgt cattgctggt
     4621 atttaaaaag acaaatgcgt tcaatatggg ggattaacaa tgaaaaaatt tcattcgagt
     4681 gctcgattcc aaaaacgact ttctcacttt tttacttact tttttctttt aatactgtca
     4741 gtcattatca tctatccgct tttgatcaca gcaagtactg ccttcaagtc aggaaatatt
     4801 gcagctttta gtttagatct ttcaagtgac tggactttag gaaactttac tcggctattc
     4861 agtgaaacac tttacgggac ttggtataaa aatacattga tcatttctgt cctaacaatg
     4921 atcgtacaag tgactgtagt aactttggcg ggctatactt atagtcgcta tcgttttatc
     4981 ggccgcaaat caagtttgat gttcttttta gtgattcaga tggtaccgac tatggcagca
     5041 ttgactgcct tttatgtcat ggctttctta ttagaagcgc tcgataagta ctggttcttg
     5101 acaatgatct atataggcgg tggaattcca atgaatactt ggctgatgaa aggatacttt
     5161 gacactgtac caatagattt agatgaatct gctaaacttg atggggcagg gcatttcagg
     5221 atttttttcc agattgtatt accgcttgtc aaaccgatgc tcgccgttca agcattatgg
     5281 gcttttatgg gaccttttgg ggatttcatg ttagctaagt tcctgcttcg aacacctgaa
     5341 aatcagactg ttgctgtcgg cttacaaaca tttatttctg atgtgacaaa ccaaagagtc
     5401 tctctttttg ctgctggggc aattttgatt gcactaccga tttgtctatt attcttcttt
     5461 ttacaaaaga actttgttac cggcctgaca tctggcggaa caaaaggata ggagcagagt
     5521 atcatgcaaa catttccgtt aaactatttt tccgcattac gtacgccaac tcaagtgttt
     5581 gcgggacgca aactattatc atggcctaaa tttttcttga tatttgtatt tttagtttcc
     5641 ttgatggtca tgcctgtgac tcttttttat gccaatcaga tccaagcgat tccattagaa
     5701 caatttctat ccgttcattc gctgatcgat gaacaaggaa cccaaaaatt ttctgaactt
     5761 gaattatctg aaactgggtt acaagcatcg caacaaacga tagctgtgac ccctgaaatt
     5821 ttggtaggtg tgtctttatc tgagaaacaa caatccgatc atggaacttt tattgatttt
     5881 gagaaggaac aatgggtgat ccaacaaaag gataaatctg gtatacgccg ctatacgatg
     5941 aattatagcc catctttcca accagattct gtacgtacgc ccgaagattt ccaacgattt
     6001 ttagaaagag aattttatgc cagtaatcgt ccaacaatca ttttatctta tagtttaagt
     6061 cttggtcttg tcctatttgt catgacgagt ctcattttat tcggggcttc ctttttcttg
     6121 tggatgacaa gaaaaagcca actttcatcc attcatacat tcaaagagag tgcaaatttg
     6181 atgctgaatg ttatgggaat aggttccatg gcagcagcag ttgtagggtt tatccacttt
     6241 gactttatct tgatgctaag tgtccaaaca acgataactg tcttgctgct tttatgggtt
     6301 tttgtcaaaa caagattcaa agataagcgt gtcgaatgac aagtttaagc gatttatgcg
     6361 acaatgaatg tgaaaaaaat gaaaaataaa gtagtttttg aaaaatttct gaaaaaatta
     6421 aacttcttag ttgattcttt tcttttttag tattaacgta atagggtagt ttttttaagg
     6481 aggaaaatca tgttagagaa aactttatac aatcaattat tttcacgttc tttctcgctc
     6541 cctgtcgaag taaattattg ggacggtaca acgaaacaat acggagatac ggacactcct
     6601 ccccaaatca aaatcaatat ccatgaagaa atcccaatga aagagattac aagtaacgct
     6661 tctttggcac taggagaagc ctatatggat catcgaatcg agatcgaggg aagtatccaa
     6721 gcattgatca acgatgctta tcagcaagca aacagctttt tacgaggaaa agattatcta
     6781 aaatggctgc caaagaaaga aaaacatacg aaacaaaaaa acaaagaaga tatccatgcg
     6841 cattatgact taggtaacga cttttataaa ctttggctag atccaacttt gacttattct
     6901 tgtgcttatt tcaatacacc tgaagatacg ttggaacaag cacaaatcaa taaagtccat
     6961 catatcttag acaaactctt tatcaaagaa ggcgaaacgt tgcttgatat cggttgtggc
     7021 tggggaacat tgatgttcac tgctaccaaa gagtataatg tcaaggcaac tgggatcaca
     7081 ctaagtgaag aacaatacga tttgattacc aaaagaataa aagaagaaca tttagaagat
     7141 aaatgccgtg tattattaat ggattaccgc gaactaaaag gcgaaacgtt tgatcatatc
     7201 acaagtgtag gtatgttcga acatgtaggt tctgaaaatc ttgaaggtta tttcaaagtg
     7261 gtaaaagatt tattgaaacc aaaaggaaca gctttgatcc atggaatcag ccgacaacaa
     7321 ggcggtgcga caaatgcttg gatcaataaa tatattttcc ctggcggtta tgtccctggt
     7381 gttactgaat tagttgataa tattacaaaa aacgatttgc aactgattga tttggaaagc
     7441 ttacgtcgtg actaccaatt gacattagaa cattggacaa agaattttca tcaagtccaa
     7501 gatcaagtca cgaaagaaat gggtgattcc ttctatcgaa tgtgggatct atatttacaa
     7561 gcttgtgcag cttcatttga atcaagtaat atcgatgtga ttcaatattt gcttgttcaa
     7621 cctggaaaca atgacatccc aatgcatcgt gcataattga ggcaaaagta ctgtttaaag
     7681 actatttaaa aagaagctaa gttttatgat gctccagaag tgaacttttt tgtctaactt
     7741 ctaggattgt ctctaaagct tagtttcttt ttctttatat tttcataata aaagtttggt
     7801 tctttaattt ttaaacatga cagaaaattt tttcttcgtt atacttgtaa tagaatacta
     7861 ttttataaca aaggggatcg aatcaatgaa aaaaactgtt atttatacta gtttatttgc
     7921 agctttgttt ttagctgctt gtcaatcaaa taccaatcaa ggcacatctg ggaatacgtc
     7981 ttccagtagc gaacagcgct cttcacaggt atcggactcc tcaacaaaag caagcacatc
     8041 ttcgtctagc cagacaaaaa gttctagtga agcaagtact cagtcagata tcaaagtgtc
     8101 tgcagctcgt gcgatcgaat tgttccaaga taaataccca gatactgcaa ttactagttt
     8161 ggaattagat agtgattggg gatcttactt ctacaagata gaaggtgtcg atgatcaaaa
     8221 agaatacgaa gcaagaattg atgctcaaaa tgaaaaaatg gagacagaga atccagaaac
     8281 tctagatcga gatgaacaaa atggtcaaaa gaaaaaagaa gatggtctgg atgttgataa
     8341 attgatcacg attgaggaag cagcaaaaat cgccgtcgac aaagtaggaa gcggtacggc
     8401 aacagattgg gatttggata aagatttagg tactacttat tgggaagtca aagtaaaaga
     8461 cggcaatcaa agtacatcag tcaagatcaa tagccaaaca ggcgaagtac tgtcaactga
     8521 acaagatgat taagaatttc ttcggaaaac aagagttgat ttttctttta gtcatgatac
     8581 actagacaaa ataacttgtt tgaaggagcg atttacttga gtttagaaac gacctttgcc
     8641 aatcatatct ttgccaatcc tttgatgaat gcatctggtg tccactgcat gactacccag
     8701 gaattagatg aactggctca ttcagaagca ggagcattta tcactaagag ctgtacgatc
     8761 aatgaacgaa aaggaaatcc cgaaccacgc tattttgatg ttcctttagg gagtatcaat
     8821 tctatgggac ttccaaactt aggtttttct tattatctag agtatgctct tgcgtatgaa
     8881 aaagtgcagg aaaaccaaaa tcaaccgtta tttttttcca ttgccgggat gagtgtccag
     8941 gaaaacttgg aaatgcttga gaaaatagaa aaaagtgggt ttaatgggat cactgaatta
     9001 aatttatctt gtcccaatgt gcctggaaaa ccacaactag cttacgattt tgaggccact
     9061 tatgaaacac tgaaagaagt attcagcatc ttttctaagc cgctaggaat caaattgcct
     9121 ccttattttg attttgctca ttttgatcag atggctgata tcttgaatca gtttccattg
     9181 acctatgtca atgctatcaa cagtgtgggc aatggtttgt atatcgatac agaacaagaa
     9241 gcagtagtca tcaaaccaaa agaagggttc ggcggcattg gcggagagta tatcaagccg
     9301 actgctttgg cgaatgttcg cgctttttat acacgtttga aaccagaaat ccaaatcatt
     9361 ggtacaggtg ggattcggac aggacaagat gcatttgaac atctgctatg tggcgcaagc
     9421 atgttacaga ttggtacaga gcttcataaa gaagggccgg aaattttttc tagaatcata
     9481 aaagaattaa cgcagattat gtcagaaaaa ggatatacgt caattgacga atttaaaggc
     9541 aaattacgca ccatttcata gcaaagagac gaaggtcatg attgatcttc gtcttttttt
     9601 ataaaaaaca ctcgtctgtt cattcctgtg caagcgtact cattgtatgt tagacgaact
     9661 tgtgggaaaa taacagaggc aaaaaggagg ataatatgaa tgatgcaaca gaattagcaa
     9721 aagagcttaa agaaaaagaa tttagttttc aagaactagt tacggatata cagaaaaaaa
     9781 taaagaagca aaatcatgaa ttgaatgcgt ttgtcgcgct tgaaccattc gagagtaatt
     9841 cccacgagct attgattccc cagtcagaaa aagggccatt ggctggtatc ccatttcctt
     9901 taaaaatgct cggtcaagaa aaaaagggat ggctagctac ttcaggatct cgtttatttg
     9961 aaacacaccg agcttctcat acatctaatt atgtgcggca agcagaagcg ataggtctag
    10021 ttccttttgg acaaaccaat gcgccagagt ttggtttcaa aaatattaca gacccagtca
    10081 tctatggccc agcaagaaac ccttggaacc ttgaatgtac accgggcgga tcaagtggag
    10141 gagctgctgc tgcagtttca tcaggaatcg ttcctttagc tggtgctagt gatggcggtg
    10201 gttctattag aatacccgct tcgttttgcg gattgattgg gctgaaacct tcgcgaggga
    10261 cgatgccagt tggtccatat gcttggagag gatggcaagg agctgctatt gatttcggct
    10321 tgacagtttc tatgagagac acggaggctt tattttatgg gatgagaagt atccattccg
    10381 gtgcgccata tcaagtatcg ccagttgagt ggcaaacaca tccaagaaaa aaacggttga
    10441 agatagcttt ttgtgttgat tcaccgatcc actcacagat tagtgaagag gcagtacttg
    10501 ctgtaaaaaa agctgtagat ttccttggaa aagaagggca tgatttgaca gagattcctt
    10561 acccactaga tggtcgtaaa ctgatcacgt cttattatca gatgaatggc gcagaaacag
    10621 cagcaatgat ccgctcgatt gaaacaggca tccagcgagc tgtcagaaaa gaagaacttg
    10681 aacccatttc ttggactttg ttccaatatg gtcagaagct ctctgccgct acttacatcg
    10741 attctctgca tgtatgggat gaagcagcaa ttgtcatgga aaacctacat caagaatacg
    10801 atctattcct ttcaccaacg actgcattta ccgcccctaa aatcagcgaa gatttgcaaa
    10861 gtgatgcgat aagagcacag atggaacaca tcagtgattg ttctgaagta gaaggtttag
    10921 acgttgtttc ttccatgttt gaggagagct tgaagatcac accttataca cagttggcta
    10981 atctgactgg acaaccagca atcagcttgc ctactcattt gacaaaagaa aacatgcctc
    11041 taggaatcca atttatggct gccagaggaa gagaagattt gctatttcaa atcggctatc
    11101 tatttgaagc aaatcatcag ttccatttac caaatccatg gaaatagcag aaacaaggag
    11161 aagaagaatg aagaaaaaaa gattgtttgc tctaataact agtgcctttt ttattttaag
    11221 cgcttgcggt acagctgccg ctaatcagga agataacagc tggagtaatg tagaaaaaaa
    11281 gaaagaatta gtagtagcga cttctggcac actgtttccc gcatcttatt ataatgatca
    11341 aaatgaatta gttggttatg atgtagattt ggcaaaagag gtggctaaac ggttaaatgt
    11401 caaaatctcc tttaaagaat acaacgtgga tggacaaatc acttctgttt caaaaagaga
    11461 atcagatttt gctgcgaacg attttggtat cacaaaagaa agagaaaaaa aattctccct
    11521 ttcagagact ttcaaatatt catttgacag tatgatcgta cgaaaaaaag atgattcagg
    11581 aatccactca cttgaagatc ttaaagggaa aaaagcagct ggcgaaccca atactagcta
    11641 tatgcgttta gcggaaaaac acggagctaa attagtcaca tatgacaatg ccacgaatga
    11701 tcagtattta agcgatgtag caaatggtcg aacagatgtt attctaaatg attattatct
    11761 gcagaaaatg gccgtatctg ctttaccaga tatccctgta aaaatactgg agaacgttta
    11821 tttcaatgaa aacgaaagtg gttttttatt gaaagaagga aatgatgctc tgaaaaagaa
    11881 aatcgatggc gtattaaaag agatgaaaga agatgggacg acaaagaaaa tatctgaaaa
    11941 atattttcaa actgatgttt cagtcaaacc gaaagaaacg atggctgagt cggtgagtga
    12001 gtgatgtact ttcccctaat tgatttttcc ctcatgtgga cttcacttcc tttcgtactg
    12061 caaggcttgg tatacacatt agggattggc ttcgtgtcgt ttcttttagg aaatttggta
    12121 gggttgcttt tgacagtttt aggtcttttg gattggctgc ctctgaatgt atttattcgt
    12181 ttttatttat cgttctttag aggcattccc gcactagtcc tgctgtttct gttatatttc
    12241 gggttacctt atcaattaag tgccttaacg gcttcagtca tctgctttac gattaccagc
    12301 agtgcattta ttggtgaaat ctatcgagga tcactagcag gcgtttcttc aggtcagtgg
    12361 gacgctggtt tagcttctgg tttttctttc tttggggtat tgaaatatat cgttttgccg
    12421 caagcttttc gaatctcagt accggcactc agtaacgtag ctatggatct agtgaaaggt
    12481 acttcgttag cagcaacaat cacagtgcca gaaatatttc aaaaagcaaa gatcataggc
    12541 ggtcggacat tcgattattt ttctctctat attttagttg cactgattta ttgggtcatt
    12601 tgtatcggga tcgaaaaagt gcaacagtat ttagaaaaac gttctatcca tcagtcaatc
    12661 tgaaaaatag aaatggtgct agcgtggctg gcaccatttc tattttttgt ttcttgtata
    12721 ttctgcctag tattttaaaa ccatttcatg taaaatgtaa atcgaaaata tgttcggagg
    12781 tagaaaacat gaagaccccg ctaaccatcg aaatagaatc ctcgttatac caatattgca
    12841 tcgagcaagg cggcttggtt gtagaagaag tcacgatgcc tgatgaccaa ggaattgtag
    12901 atacgcttag ctgcttgaca aaatttgatg gaagccgaga atggcgatgc tatgagctga
    12961 aagtgacaaa agcagatttt cgctctacag ccaaactgtc atttgttgga cattataatt
    13021 attttgtttt accaagtaaa ctatatgaag aagtaaaaac tgaaatccct acaggaatag
    13081 gtgttttagt gtatcgtccg tacacggttg aagaggagat gcctgcctct ggtacatttc
    13141 tgattgcgaa aaaagcagca aagaaagagc ttgctgtacc ggaagaagct ttgacaaacc
    13201 gcttcatggc ttctttattc agggaagtaa gaaaagcaaa gcaaatggag cgaggcgtaa
    13261 aatattttcc aaaagatcaa ttgtacaaag aattgaaaag acgttcagaa aatcaagatc
    13321 cattttctaa cgaacgatat tatgaacggt ttttagagga aatagctagt caggcaatca
    13381 atgagttgca agaagaagtt atcgcgcttc agcatgatta tgaatatttg aggcaacaac
    13441 agcagatccg tcggttgcct acagagccat tggagtgaaa aatatgtact atccttattt
    13501 aagaggaaaa caatttgacc tattagcatt aaaagaagca ctgtcgcgag gattgctctc
    13561 caataaaatc caaccagtga tcgaaccagt acgtgattcc gctacgctta aaaatgtgat
    13621 cgaattattt caaaaaaagc accatccaat cgctgtgatc caaaatcccc aagtaggaca
    13681 attcaaatta ttcgatcagc atgtgcacag ttggacagtc aaagaaaata gctctgtcgt
    13741 ccctgcacag atcttaacgc cagaaaatct cagtgaagta ctggattcac ctccagcgtt
    13801 tcttgttttc gatggacagc actacccaaa agatacagag gtgtggaaac agttggcagg
    13861 agttgattcg aaatttttga taccagatac tagtcgcttt cgcatctggc tgcccgaaaa
    13921 caaaattgtg atccgtgact cttttcaaac gagaaagcat gttgaaagtt atgcagataa
    13981 aacagatgac ttttttagtg atgactatct ttttttccac gcagacggat atattggatt
    14041 ttctgatttc acgatcgaag gtagccgtta tttcgataaa gggttcccta gccgagcgct
    14101 tgctatccat ctaacgtata ttgatgctta cggcaatata cgcgtgaaac attttgtctc
    14161 tgacagtaac gactctgcta aagaccaagc attgaagttt cttgaagcag gagataaaat
    14221 gaaaaaatgg atcatgcgtc accatcatca actcttgatt acttcaggaa tgattgaatt
    14281 attggctctt taccagcaac aaaaattccc tggactggga gtgttgaaaa agtggtcact
    14341 tatgcatcat ttggaactta ttagtcacct gctagatcat ccaaaagatt ggcttaaata
    14401 ttattcaaaa gttcaggaac tatatgaagt gatccaatga ttagataatg taaatgaaaa
    14461 aggaatagaa aagaggaaac tggatgtata ttcggcaaac aaggaaagaa gaagtagaag
    14521 aagtggtaga aatcattaat gccgcagcgg atttcattgc ttcgcaaggc tctcctcaat
    14581 ggcaaaacgg gcagaaacca acaaaagaaa cgattttgaa cgatattgaa agaaaagaaa
    14641 gctatgtatt aatagtggaa gatcaaattg ctggaacagc tgcattagtc tcaggcgtgg
    14701 atcccgttta cacaaaaata gatggtagtt ggaaagaatc aactggtcct tatttatcga
    14761 ttcatcgtgt cgctttagca gaaagtactc gaggaaaaaa actagcaaaa ccgttcttag
    14821 aacatttgtt gacgattgca gccagcaaaa atatccaaga tatccgtatc gatacttatc
    14881 cagctaatga accgatggaa aagaccatct attctgctgg attcacttat caagggatga
    14941 ttgaatttcc ttttgaacac ggcgaaagaa aagcatatca aataatcatc aattgactat
    15001 aaaatcatct gagcaaagat aaagacatta acactactga aaaaaacaat catatgatag
    15061 ccaagaggtt gtaacagaag tggccagctt caaaaaacaa gaaagaattt ccgaaaatgc
    15121 ttctcaaatt tttgtggaat tttagcgtat tttacgaaat agctgctttc tgaaactccc
    15181 ttcatcgaga ggtcgtgaca tagcttttgt cacgatctct cggcttatcc tacgagtatt
    15241 attcgataca tttatatcaa taatcctttt tttattttat ctgcagcttc ttggaacgct
    15301 ccatcgtttg acacgactaa aagtgagggg atagctttca aacgtttcaa ctgtgctaaa
    15361 tcttctacat aaaccgccag tctggctgaa atctgttcag atgaatctcc acgttttttc
    15421 aggcgcgatt gcaatgtttc tttagagatc gtaataaaga taggacggac taccgtcccg
    15481 aaatactcat ataaatgcca aaaaccattt gctgtcaaaa cggcgtaaca atttcctact
    15541 gtcagtttct ttttaatttc tgctttactg gaaccatatt tatgttgagc atattggtcc
    15601 cactctgcaa actcatttcc cttgatcatc tgattgaatg tattgtcaga aacaaaataa
    15661 taatcctctt gatctttttc cccaggtctt ggtgatcgat tcgtgtaagt aatgatcttt
    15721 tgttccggct gaaaaatagc ttctgcgagt gaagtttttc cagaaccaga aggaccgata
    15781 aatacatagc aataattcat tgtcatttct cctttgatga ttgaacaagt gaaaaagctt
    15841 tggaaaatat ttccaaagct tttaaaagta actttgcttt tttgtatttt ataccagcaa
    15901 gccagtcaca atagcactta aaaagctgac taaagtcgct gttagcaaaa ttttcaatcc
    15961 gtgttttgcg acaagatttc ctttttcttc atttaatccc ttcatcgcac cagaaatgat
    16021 cccgatagaa gagaagttgg caaatgaaac gaggaagaca gatataattg ctgttgtacg
    16081 ctcagtaaaa tggaaattac catttgctaa ttctgtcata gcaacaaatt catttgtaac
    16141 cagtttagtc gccatgatac taccggcatc cactgcttct ttccatggga ttccgctgat
    16201 gaaggcaaga ggggcaaaga tgtaacctag gatctcttgg aaagtgatgc cgaatatccc
    16261 gtggaagata gcattgatca tggcaatcaa agcaacaaaa ccgataagca tagcagcaac
    16321 cgtgatcgcc acatggaaac catcaagaat gtattctccc aacatttgga aaaatgtctg
    16381 ttctttgttt tcttcaatga tcagctcatc ttctttttct tctaatacat agggatttac
    16441 aattgaagcc agaatataac cgccaaataa gttcaaaaca agggcagtga tcacatattt
    16501 agaatcgatc agtgccatat aagatccgac gatcgacata gaaactgtag acatagccga
    16561 tacactcatg gtgaacaaac gtttctcaga taggaagggt aattcctttt ttatggaaat
    16621 aaaaacttcc gattgtccta aaatagcaga agctactcca ttataagatt ccaatttgcc
    16681 catcccgttg attttactta atcctagacc aacagctttc ataaataatg gaagaatctt
    16741 gatatagcgt agaataccga taatagcaga aataaataca attggcatca atacggaaag
    16801 aaagaaaggt gcacttcctt tatttgctac accggcaaaa acaaagtcaa caccttgccc
    16861 agcaaaagca agaagatgat caaacatttt tgccaataca gaaacaaccg ctgtaccaaa
    16921 agttgtacgt aataatacga aaccaagaac aagctgtaaa acgaacatca caattagtgg
    16981 tttcaatttg attgcttttt tgttactgct gatcaaaaaa gcaagcccta atgtgacaaa
    17041 taaaccaata atacctaata aataactcaa ataatcactc ctattccaaa ttccgtttta
    17101 ttttacctgt tttgcaaata aaatactaat ctttagtgaa attttcatta cttttttaga
    17161 gtgaaacgca ttcattttcc attagaaaat aaagcgaaca aaataaaagg tttaaaaagt
    17221 gtaacgtctt agtaaagtct ataaaaaaac gaaactatcg attaagctaa tagtttcgtt
    17281 ttcaacattt ataagtaaac tttttctatt ggtaatatag attttcggaa gggtagacag
    17341 gtgcgcaggt aatatcgatt cctagttttt tcaatgtttg ttcatcattt ttattgacca
    17401 tcacagtaga gtgggcttgt gctccctcaa gttcagctag tttatcataa gcaacttgag
    17461 cgattggatt cgttactgca ctgattgata aagcgatcaa gacttcattg atgcttagtg
    17521 catccaaaga ggaatgaagt gtctttgatt tcaatccttg tattgtttca agaactaatg
    17581 gtgacaataa aggaatgtca tctgcgatat gggctaaatg ttttactgca tttaagataa
    17641 ctgccgctga cgcatccatc aatgaacttg tacgcccggt aatgatgact tcatctggta
    17701 attcgattgc aatcacagca gaaggatctt ttgtaggact ttgcgcttga atcttatctg
    17761 catagtcatg ggcagggact accacgtttc ggtcttcagg acgtaaaccg acttcttcca
    17821 taatcagttt gattcgatta actgcttctt cgtcgatcaa tccttttttg aagtcgcatt
    17881 ctgtttggaa ataacgacgg atgatttctt gtttagaggc ttcttggaca acggtatcat
    17941 ccacgatccc aaatcctaca cgattcacac ccatgtcagt aggagattga taaacagatt
    18001 ctttaccagt aattttttca ataatacgtt tgattacagg aaatgtctca acatcccgat
    18061 tatagttcac agctacttta ttgtaggcat caaagtggaa ggaatcgatc atattgacat
    18121 cttttagatc gactgtggct gcttcataag caatgttcag cggatgtttc aacggaacat
    18181 tccatactgg gaaagtttcg aacttggagt aaccagctgc atgtcctttt tggctttcat
    18241 gatacagttg gttcaagcaa gtagcgagct ttccacttcc tgggccagga gctgtcacta
    18301 caacgattgg tttagttgtt tcaatatact cattttttcc aaaaccatct tctgagacga
    18361 ttttttctac gttgactgga tagtcttcga tttctgagtg tttatagacc ttgatgtttc
    18421 gtctttctag tttattgata aagactttcg tggcaggctg tccactatag cgagtgataa
    18481 cgacactgtt gattaataag ccataaccac gtaattcatc gattaaccgc aagatatcca
    18541 tatcataagt aatcccataa tcaccacgga tcttattacg ttcaatatct ccagcataga
    18601 cgcaaatcag tatttcagct tgatctttca atttttgcag caatttgatt ttcgcatctt
    18661 cttcaaatcc tggaagtaca cgtttcgcat gcttgtcgtc aactaacttc ccaccaaatt
    18721 ctaaatataa tttgtcatag tggttcactc gttccaaaat atacgctgac tgctcttcaa
    18781 tatatttttc tgaatcaaaa ccaatttttt tcactaggtt ccctccttaa gtactaaagc
    18841 attttttcat ttttttgagg attcgtcaac tggaagagcg aaattcctca aaaaaacaaa
    18901 gaaaatggtt taaaccaact tgtctagata gttattcatt ggtatgatag tttttgataa
    18961 ggaaggtgct acatgaaaaa aagacagaaa aagaaaaacg catataaaca ctatatccgt
    19021 tcgattttta ccggttatga aagaatgctt gaagatcccg aattggaaca attgacgttt
    19081 acttatctaa acgaagaaac gcagctaact cgtgatgagc atcagcgcat tcatttcaca
    19141 acaagagatc tgcctagtaa ataaaaaata agataaaggt catcggattc gattctgtcc
    19201 tatcagatgg aaagactagt tgatcgaaaa aaaggctgta ccaaattttg gtgcagcctg
    19261 ttttatggag cattgcccgc tcttttttat aacaattttt tacagaaaat ggtactattg
    19321 aagtattaat aagtgaaaaa cagatgagta agaggaggca agtagtatga aagagccaga
    19381 tacaaatgca aattatgagc cagacgaact agaggagacg aacgcggatc aggatacagg
    19441 acaagaatcg tcttcttttt acgatatcaa agaaaaagca agcatgaatc tttcccaatt
    19501 attagaagac ttacaggctt ttgaaaaagc tgtccgcgaa gaagatatcc ctgagatcta
    19561 ccgtttatac aatggccgat tgaatgaaga attaaaaaga agctcaaatg aaaatcacga
    19621 gatcgatcaa ttgctgatgc gtaagatcca tgatcgtttt ttacaggaat ttccttttat
    19681 ggaacaagta gaaaacatct cgccaacgat gagctattac aaaatcggca cttattacca
    19741 tgatcggccc acggtcggta ttgatgcaag tctgccagaa atttttgttt tgcctaagat
    19801 cgatgaagag tgggagaaat attcccaacc ggagtcaacg gactatgaca aaaaaatcaa
    19861 tgagctagac gcaaaggtga tcactgctca gacagaaatt gaacgtttag atgaacaaat
    19921 aaaagaaatc aatcgacaaa tgactgattt gaacgacaat aaaggattct tgaatcgtaa
    19981 aaaaatcgag gaagaaattc aagaattaga gaagaagaag caagtactat ctaatgaaaa
    20041 attaggctgg cttccttata ttgagacgcc tgaaacaatc cagcagcaaa aagaagcatt
    20101 gaagcaagaa gcaagagcag accagcttcg tgcagccatc gtagaaaaag aacaaaggca
    20161 aatcagacgc tatttcggta gcaaagaggg attcggtcaa gcaatccatg aattcttgat
    20221 gaattatctt ggcaatgaga atcctaataa tcaatctaat gaaggaggtt ccgagtatga
    20281 atgaacacga tgaagcagaa tggacaaaaa atgaagaaca aacagaagat catgaagaaa
    20341 cgaacacttc attggaagaa caaacaagta atcctctgga tgacacagaa gaaatcccta
    20401 tgctagagga aacaaaaata catgaaaaag aaaaagtaac tccgacgaat acgaatactg
    20461 cagaaaacac ccatgcttac gaaataactg aaaatcccgg gtatttagaa ggaaaagagc
    20521 aagatcatca agatatcgtc agtttgattg aaacagcgga agcagaatta gcggatttac
    20581 gtatgagaaa agatttgatc gaatcagatt ttccacaatt attagaatca tatgatcgtt
    20641 ttatattgaa tgaagaacca atctccacga tcggaaagac aagcctgatc gaatacttca
    20701 cgtatgaatt actgaaaccg ttgaatgata ttggtctgga acggtcaaat tcagcttatg
    20761 acacatggaa aacaagacat ttttcgatca attatacgtt gaatggacaa aacgaattag
    20821 tcttttcctt tgtattaccg accatcgatc aacgatacca agtggaatcg atgagtctcc
    20881 taaacgtatc accagagaca atggaaatca atgtccaaga tgaccgggtg ctttctttga
    20941 tccgacactg gtcagtggat cgagtattct ctgcagggca gatcacgata ttcaatcaca
    21001 aattgaatca attattagcc catgcaagaa cacttggttt taccgttaat cagacattac
    21061 tagacaatac gaagccactt cgtttgaatc ttcagagcga atttgaattg acgaatgaag
    21121 tattggatga tatttttatc gtggcaatga aaaatccatc ctacgatatg gaaaagatca
    21181 acgaaactac ttatcaagta cgattagata aaggacagtc tttaacgata acaacaaacg
    21241 aaaaagatca aacagtcttg tcgatctcat ctggagacta cccacgatct gtcattgatt
    21301 tctttatcaa ttacgaattc ttggtcccat taatggtgag aaagtcctga ttggataagt
    21361 tggattacca gatgtcattc agataattct gttccaaagg acttgcggca gttatccaga
    21421 ataattgtca caagtctttt ctgtatgtac taaatggacg tgaataactt gctgatgaag
    21481 gagggatttc attaggaatg tccacaaata aaatagcaca gcttagctta ttgtctgctg
    21541 cttgtgtagc aggtaggatc gcttttcaat ttattccgaa tttccagcca atgactgcta
    21601 tttttttatt tatagtactt tacttaagct taaaagatgc gttaattgtc atgagtcttt
    21661 ctattgcgat cagcagtttt tattttggtg ttggaccatg ggtcatcggc caatggatca
    21721 gctatactgt tgttctgagt ttgttttcaa tcgtctgctt gagaaaaaca gtgcaaagga
    21781 atctatggtt aaagggagtc tgtttttttc tggcgggcat ggtttacgga ataggtatga
    21841 ccgtttttga tacgctgtta tatcgtttgc ctcaaccttg gatctattac cttcaagggg
    21901 tgtcgtttga tttgatgcac agtataggca atgttgtgtt ctttcttgtt tttctgccag
    21961 tcgtcaaacg tttttacaat cattcaggag gaaaaaatga aaaaaataat ttatagtttg
    22021 cttgcaatga gtgtcagtat actcattttg acaagttgca gtacaaccta tacacaatcg
    22081 gcttccaacc aaaaggagga agtaaaaaca tcgcaaaaag caacaatcac gttacaagaa
    22141 aatggtcaaa acttttcgga aaaagaagtg gtcttcaaaa aaggcgatga tttactgagt
    22201 attttgaaac gaaattttga agtcaaagaa gataacggct tcatcacttc gcttgaaggg
    22261 cactcacagg atgagaaaaa tcaactctat tggatgttta ctgtggacgg gaaaagtgct
    22321 actactggtg ccaaagagat caaattgaat gatggacaat cggtcatttt caatttgtct
    22381 aaactataag tttcataaaa aactgttaaa gggcgaatat tgccataatt cagtgagttc
    22441 agttgatgaa agaaggaaaa taacatggaa aattttaaaa aaagcggaat gttttccgta
    22501 attgctgctc taggggctaa tattctagtt gctatcagca aatttgtcgg ctatgcaatc
    22561 agtggcagtg ccgccatgct aaacgaaagc atccacagta tcgttgattg cagcaatcag
    22621 attttgctgt tattcggtga taaacaagcc aataaaggac agagcgagtt gcatcaattt
    22681 ggtgaaggac gagcaaaata ctttttcagt acaatcgtcg caatgatgct tttcttcggc
    22741 ggtggggcac ttggggtgat ggaagctttt gaaaagttga cgcatccatc ccatgaagtg
    22801 ggtaatccga tcattgttat agcaatactg ttatttggtt tggtaatcga aggaagttcg
    22861 ttacgcgtgg caatgaaaga aatcaaagaa ttgaatactg aaaaactttc cacgatgcgc
    22921 tttttgcgcg aaagtcgaca cagtgaaata ctgattattt ttacggaaga cttgtgtgct
    22981 gtaatcggtt tggtcttggc tttagcagga acaatattga cttatgcaac cgggattgct
    23041 gcatttgatg cgatcagcgg attgctgatc ggtttcttgc tgatgggagc cgccattttc
    23101 ctagcaaaag aattttatag tctgatcatc ggtgaaagcg tgacagcaag cgatttatca
    23161 aaaatcaagt tagcttttga acgtcctgag atcagtcggc tgatcgatat aaaaacagtt
    23221 catttaagtc caacggagat tttagttgca gcaaaagcag atatcgttgg taacaaagag
    23281 aatgaagctt actcggtcat caatgatatt gaaaaaaata tgcgtcagtc tcttcctgac
    23341 aagaagatgt atatatacat tgaaacagat aaatatgacc caaattattc tcgtaaataa
    23401 aacgtttatt catgaattga agcaaaaatg agaaaaatat aatcttgttt ttaaataaaa
    23461 cggctacaat ttagcgaatt tatgttacaa tgagtccgct gtttatttgt tgtaattaaa
    23521 ggagatattt taatgaacaa aaagaaaagt gcgccatggc ttcaagcttt gcatgcagtg
    23581 atgccacttt gtataagcta tataccagta ggattagctt gcggagtatt gttgcaaaaa
    23641 gttggatttg atcctctctt atcaggactg ttatcgattc tggtcttttc cggtggcgcg
    23701 cagtttttag ttgcatctat gcttacgacg caagcatcat ttgcaacgac attattgatg
    23761 gttttctttt tagaattacg ctatacatta ttaggagcaa gtcttgccgg ttttatgaaa
    23821 aaagaaaagc ggacgttttt ggctgtcttt tctcaatcac tgaatgatga aaactatgcg
    23881 gtgaactact taaagttttc aactgaccct tcctggaata aaagaaaggc tttatatgtc
    23941 aattggttct caatgactgc atgggccggt agcaatatga tcggtaattt tttcggctcg
    24001 gtgattcgag tagatgctga ccttgtccat tttgctttga cggccatgtt cattttcatg
    24061 tttatcatgc agatgaaaag cgcactactg atctttacag gtttattctc aggcgtatta
    24121 ggggtcgtat tcatggtatt attccaaaat actttcggat tgattgcagc aacagtcatt
    24181 gcttcatttg ccggatttat gctggagaag gcattcaaaa aagaaaaagc taagaaacta
    24241 agagaccgcg gtaaagatgt caatgagccg ctcttcaact tcccggaaca ggaggccaat
    24301 caccatgact aaactatatt tgctactttt agttgcttgt ctgttcatcg tggcatatat
    24361 cccgcgcttg ttcccgatgc tttattttac tcatcgaaaa gtgccatcct ggttcagtga
    24421 ctggatgaaa tatgtaccgg tggcattatt tgcggcgtta gcttttaaag atgttttcat
    24481 tactcatgaa catctggata ttgcttggaa tatcaaaata gctgcaatga ttctagtagc
    24541 aggcgtggct tacaaaacac gttcgatggc gttgtcagtt ttgacaggac ttgcgtctgt
    24601 ctttttgctg tctatgcttt aaactgtttt attttattaa aaacagagat tgcgacataa
    24661 gttgttcaaa ctgatctatc cgaactgcat ttgcaacagt caattctgga tgcaagttta
    24721 ttcgtaggga taaaaaagat tagtgttggc ttatgatgca agctcttttt tattttagga
    24781 attagagagg gatttgtcct tctatgattt tttgctataa tagggaagat gtttatttta
    24841 tgaaagaata gttgagggaa tttaaatgaa taaaaaattg atgcaaggaa cattttggct
    24901 aacctttgct aatcttctat gtaaagtttt aggggtagtc tatttgattc cctggctaag
    24961 tatgatgggg aacaatcaag atggtatgct tgctacgaca ttgtataatg ttggctatct
    25021 tccttatgga ttgttcctga tgcttggtac agtcggattt ccaaatgcga ttgcaaaaaa
    25081 agtagctgta gccacaaaag aaggagataa cgctgcttgt cggctgattt ttagaagtac
    25141 gatcaatatc atgtttgtca tcggtatcgc ctctgctgca ctgatgtatt tatttgctcc
    25201 tttactagcc ggaatcagtc cgattgctaa cgttaataat gggatactag ctatcagaag
    25261 tttatgccca tctttgattg ctatcccaat tcttagtgca atgagaggat atttccaagg
    25321 aaaaaatgat ctgcggcctt atggaacttc tttgattatc gaacaggctg tgcgagtgat
    25381 cgttatcttg gctggtactt attacttgcg cgtattgact gacggaacca tattagaagc
    25441 tgtattgatc agtacagtcg cttctttctt cggtgggtta gctgcgatta tccatatgta
    25501 tatcgtcgga cgcagaaaag acttcttcga acttaaagat tttttgattt ccagccgtta
    25561 tttcgaaaga gaaaaccgat ctgcctctgt ctctatcatt cgggaaacac taccgtttat
    25621 ttttgtcggc tcagtcatta ctatcgttca aatgatcgac caagtaacga tgaaaccact
    25681 gcttcacttt ttccgtccag agatcgcaga tcagcaactg gaattcttat tcagccgtgc
    25741 ttctgtcaat ccaaataaat tgacattgat cctgatctcc atggtaggaa cagtagctat
    25801 aagcagcttg ccaatattaa gcacgatgaa gaaaaaagat cggatacaaa tcgaaaaaac
    25861 agtaggagat agtttttcga ttgcgttatt gattcttctt ccttcattga ctggaatgtc
    25921 tttattagct ggaccactat acacattgtt ttttggctat gatcctgaaa gtgtcggata
    25981 tttccaaatg gctttgctag cttctttgtt tttctcttta ttcacgatcc tatctacgat
    26041 gcttcaatct ttgaatcacc atcgttttgc gatccgatta actgtagaag caattatcct
    26101 gaaagtcatc ttccaagtaa tcggattagg attagttggc ggatacggga tgagcttatc
    26161 aaatggctta gcattcggta ttgtctttgt tcgcggctat cactttatgt gcaaagaata
    26221 ccgaatagca cctttagcaa agatcagcaa ttttttctta aaaactttcc gctccaccct
    26281 gattatgctt gcagtatgtt ttgccgtttt ctttttgctg aatcagcgat tgtcaatgga
    26341 atcaaaaagc tatgcaatga tttattgtgt ggctgtagga agtattggcg gcttagtatt
    26401 tttattttct caattcggaa aaaatgggtt tcgaatgttg aaaaatgtta aacatcatag
    26461 cagaagcaaa gaataggtta actaatcgtt ttttgctgtc acttgaaaaa cggcaactcg
    26521 tatgattacg cttgtcgttt ttctattttc caattttagt ttcatatcta atcttactta
    26581 tgatatagtt ataaggtctg gaaaaattct ttgaaacgag gaaattaaac acatgaaact
    26641 tgcagtagtt acagatagtt cggcatatct gccggaacgt atcaagaacc atcccgactt
    26701 attcgttatt ccaatccctt tgattatgga tgggaaaatt tataacgaag gaatcgatat
    26761 cgaagcggat gaatattatg gacttttaaa aaacagtaaa gattttccta ctacttcaca
    26821 acctgtcgta ggggaagttt tgggtttata tgaggaattg aaagagaaag ggtatgacac
    26881 gattttaagt atccatcttt cttcagggat ttctggcttt gtcaacacat tatttgcgat
    26941 gaaagatgac atcagcggag taacggtcat tccttatgac tctaagatca ctagtatgcc
    27001 gatgggacac atggtagaag ctgccttaga cttaaatgat gaaggtcagt cccttgatga
    27061 aatacttgca catatcgatc gaatccgaga taatacctat gcttatctga ttgtagatga
    27121 tttgaacaat ttagtacgag gaggacgttt gacaaatggt gcagcgctga ttggcagctt
    27181 attaaaaatc aaaccgatcc tgacattttc tgaagggaaa attgttttat tcgaaaaaat
    27241 ccgttcaagt aaaaaagcat ttgctcgagc tgaagaaatt attggtagaa gaaataacga
    27301 aatacaaccg ccagtcaaac tttacgtgat acatgcaaat aatttagaag tagcgattgc
    27361 ggagaaagag aaattgcaga aaaaatatcc aaatgcagaa atcgaaattg gtcatttcgg
    27421 accagtcatt ggtacacatt taggtgaaaa agccatcggc attgccattt cagcgcaata
    27481 attcctttta ttcaaaaaac ataccaaaaa tat
//
LOCUS       DACDEG010000026        26901 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.026, whole genome shotgun sequence.
ACCESSION   DACDEG010000026 DACDEG010000000
VERSION     DACDEG010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.026"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(85..432)
                     /gene="rbfA"
                     /locus_tag="IV142_001217"
     CDS             complement(85..432)
                     /gene="rbfA"
                     /locus_tag="IV142_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP8484886.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(459..2765)
                     /gene="infB"
                     /locus_tag="IV142_001218"
     CDS             complement(459..2765)
                     /gene="infB"
                     /locus_tag="IV142_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP8484887.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(2778..3089)
                     /locus_tag="IV142_001219"
     CDS             complement(2778..3089)
                     /locus_tag="IV142_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP8484888.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(3086..3379)
                     /locus_tag="IV142_001220"
     CDS             complement(3086..3379)
                     /locus_tag="IV142_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP8484889.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(3401..4576)
                     /gene="nusA"
                     /locus_tag="IV142_001221"
     CDS             complement(3401..4576)
                     /gene="nusA"
                     /locus_tag="IV142_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP8484890.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(4600..5073)
                     /gene="rimP"
                     /locus_tag="IV142_001222"
     CDS             complement(4600..5073)
                     /gene="rimP"
                     /locus_tag="IV142_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP8484891.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(5217..9569)
                     /locus_tag="IV142_001223"
     CDS             complement(5217..9569)
                     /locus_tag="IV142_001223"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP8484892.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            complement(9781..11490)
                     /locus_tag="IV142_001224"
     CDS             complement(9781..11490)
                     /locus_tag="IV142_001224"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP8484893.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            complement(11558..12826)
                     /gene="rseP"
                     /locus_tag="IV142_001225"
     CDS             complement(11558..12826)
                     /gene="rseP"
                     /locus_tag="IV142_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP8484894.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(12987..13787)
                     /locus_tag="IV142_001226"
     CDS             complement(12987..13787)
                     /locus_tag="IV142_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP8484895.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(13784..14596)
                     /locus_tag="IV142_001227"
     CDS             complement(13784..14596)
                     /locus_tag="IV142_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP8484896.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(14791..15348)
                     /gene="frr"
                     /locus_tag="IV142_001228"
     CDS             complement(14791..15348)
                     /gene="frr"
                     /locus_tag="IV142_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP8484897.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(15351..16073)
                     /locus_tag="IV142_001229"
     CDS             complement(15351..16073)
                     /locus_tag="IV142_001229"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP8484898.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAHMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(16209..17090)
                     /locus_tag="IV142_001230"
     CDS             complement(16209..17090)
                     /locus_tag="IV142_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP8484899.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(17189..17971)
                     /gene="rpsB"
                     /locus_tag="IV142_001231"
     CDS             complement(17189..17971)
                     /gene="rpsB"
                     /locus_tag="IV142_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP8484900.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            18330..18809
                     /locus_tag="IV142_001232"
     CDS             18330..18809
                     /locus_tag="IV142_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP8484901.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            19026..20117
                     /gene="phnW"
                     /locus_tag="IV142_001233"
     CDS             19026..20117
                     /gene="phnW"
                     /locus_tag="IV142_001233"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP8484902.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            20110..20895
                     /locus_tag="IV142_001234"
     CDS             20110..20895
                     /locus_tag="IV142_001234"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP8484903.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(21241..22932)
                     /locus_tag="IV142_001235"
     CDS             complement(21241..22932)
                     /locus_tag="IV142_001235"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP8484904.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(23354..24301)
                     /gene="arcC"
                     /locus_tag="IV142_001236"
     CDS             complement(23354..24301)
                     /gene="arcC"
                     /locus_tag="IV142_001236"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP8484905.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(24416..25435)
                     /gene="argF"
                     /locus_tag="IV142_001237"
     CDS             complement(24416..25435)
                     /gene="argF"
                     /locus_tag="IV142_001237"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP8484906.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(25526..26755)
                     /gene="arcA"
                     /locus_tag="IV142_001238"
     CDS             complement(25526..26755)
                     /gene="arcA"
                     /locus_tag="IV142_001238"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP8484907.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         7169 a         5811 c         4780 g         9141 t
ORIGIN      
        1 tttgtacaac ccatcagtcc ttattgacaa agagccaaaa aatcccgcaa cccctgattt
       61 cacaggattt gcgggatttt ttgattagtc tttgttcaag tcgcgaagca attcatcgat
      121 atggttccca tagacaactg attcatcaag ttcaaagaac aattcaggtg tcttataaat
      181 acttagattg tgccctaact ctcgacgaat caatcctgat gctttattca atccatcttg
      241 tgcttttttc ttatctgatg ctaagtcaga taaaatgctg taatagatcg ttgcttgctg
      301 taagtccccc gtaactttca catctgtgat cgttacgttc tccacacgag ggtcacggac
      361 ctttttgcgc aaaatatcat tgacttcttt taagatttct tgaccgactc ggcgatcacg
      421 gtagttagcc ataatgattg gcctcctttt atcagttttt agtcattttt gacttcttcc
      481 atgataaatc cttcaattac gtcatctact ttgatgtcat tgaatttttc aatcatcgca
      541 ccacattcat aacccatttt gacttctttc acgtcatctt tgaatcgttt cagacttgca
      601 agttggcctt catagattac gattccgtca cggattacac ggacaccgct atctctacga
      661 atatatcctt ctgttacata ggcacctgca atcgtaccaa ctttagaaac tttgaaggtt
      721 tcacgaacga tcatttgacc ggtgattttt tcttcaaatt cagggtctag catccctttc
      781 atcgcagttt cgatttcttc gatggcttta tagatgatac ggtgaagacg aatatcgact
      841 tcttcagtat cagcttgcac ttttgcctgc ggtgtaggac gaacgttgaa accgatgata
      901 atagcgttgc tggctgctgc taaggttaca tcactttcat tgattgctcc gacagctgaa
      961 tggacgattt ttacacggac accttccaca tcaatcttct tcaatgatgc agccagtgct
     1021 tctgctgaac cttgtacatc tgctttgatg attacgttaa cttctttcaa ttcgccttct
     1081 ttcaggcttt caaataagtt gtccaaagtc acacggcttg tggcagcacg gttttccaat
     1141 aatgcacgtt ttgcgcgttc ttcacccgct gcacgtgctg tcttttcgtc ttcgaagact
     1201 acgaaacggt cacctgcttg aggtacatca ttcaagccag tgatttctac tggcgttgca
     1261 ggtcctgctt ctttatcgcg acggccaaga tcgtttgtca ttacacggac acgtccgtaa
     1321 gtattaccga caacgattgg gtcaccaacg tgaagtgttc cttcttggac aagtaaggtt
     1381 gacacaggac ctttaccttt atcaagacgt gcttcgatca ctgttccgat tgcgcgttgt
     1441 gttggatctg cttttaaatc ttctacttct gcgactaaca ggatcatttc cagcaattct
     1501 tcgatgtttt ggccgaattt cgctgagatc tcaacaaaaa tcgtttctcc accccatgct
     1561 tcaggaatta attcatactc acttaattct tgcataacat gctgaggatt tgcacctggt
     1621 ttatcaattt tatttacagc gacgatgatt ggaacgcctg cagctttggc atggttgatt
     1681 gcttcaactg tttgtggcat gacgccatca tctgcagcaa caactaagat cgtgatatct
     1741 gtgatacttg ctccacgtgc acgcatactt gtaaaggccg catgtcctgg tgtatccaag
     1801 aaagtgattg gtttaccatc aatatcgatc tggtaggcac cgatatgctg tgtgattccg
     1861 ccggcttcgc cgcttgttac acgtgaatgg cgcaatgtat ccaataaggt tgttttcccg
     1921 tggtctacgt gtcccataat cgttactact ggcggacgtg agactaattt atctggatcg
     1981 acttcttctg cctcgaagaa tttatcgata tcagcaatgt caacttggac tttttcttgt
     2041 ggttccatgc catagtccgt cgctaacagt tcaatcgtat ctttatccag tgcttggttt
     2101 tgattgacca tgacaccaag cataaataat tttttaatga tttctgctgg ctcacgatga
     2161 atttttttcg caatatctgc tacgttcata ccttcagtat actctaatac atctggcaat
     2221 tcacggaatt tgcgtggtgg tacagctggt ttagttgatt gttgctgttt gcctttttta
     2281 ccttttttat tataacgatt acggttatta ttattaaatt tgccgcgatt gttattgttt
     2341 ggtttgttgt tattgttagt acggttttga ttgccgcctt ggctcgttcc ttgacgattt
     2401 tggctgctgg tatttcccgt attattttgg tttgttgttg gtttttttcc ttgattcact
     2461 tgaccgctcc cttgaccgcg atcttgataa ctgcggttat ttttattttt ttgttgattg
     2521 ttcgttgttt cttttttacg ttgatcgttc ggctgttgat tggctggttt tctgcttgct
     2581 tgttgtgcag cttgttgttt cgtgcttggg tttttgaacg cttgttgcaa tttcttctca
     2641 tcactgctag taatcgcacc catatggttt ttcacatcga tgccaagttg ttgtgctttt
     2701 tccacgacgt ctttactaga ttttttgagt tcttttgcta attcatagat tcttttgttg
     2761 cccatgcaat caccttccta accttcaatt agctccttgg tcttcttcgc aaatccggca
     2821 tccattattc cgaatacctt gcgaggtttt ccgatcatct gcgtgatttc tgcttcagaa
     2881 aatagctcaa agcaaggaac ttcataatat gaactcttat cttttatctt ttttcttgta
     2941 ttctcacttg catcggttgc cacaaacacc aattttgctt tttgtttgcg gatttcagca
     3001 atggtcagtt cttcacccgt gattagtttt cccgcgcgca tcgctagacc gatgagattc
     3061 atggcttttt gccgattaac tgtattcatt ttccaaataa ctctttacgt gctttttggt
     3121 gagtgacata gtcaagaagt tcttgataaa actcatctgt taatgttgtt tcgagtactc
     3181 gatcgagaat ttttttatcc caagcttgtt gtacttcttc cggctcaagt gagacatacg
     3241 ctcctcgtcc aggcattttt ccagttggat cgatcgatac ttccccttct tttgaacggg
     3301 tgattcggac catctctttt ttaggtttca tttcacctga aaccacggat ttgcgtaaag
     3361 gaattttcct ttgtttcatc tctttgcctc cttactaaag ttaagcttct ggtttttctt
     3421 cgaccgtttc attgttgaat gcaattgttt catattcatc gtctgttaaa tctgactgaa
     3481 cgattgcttc atcatgcagt tcttctgttt ctttttgttg ttcttgattt tcataaaatt
     3541 ccgtcatgtc agattctgat ttgatatcga ttttatggtt agtcaattta gcagctaaac
     3601 gtgcattttg tccgcgttta ccgattgcta gcgataattg gtagtctgga acgacaactg
     3661 tgcaggcctt tggatttgtc tcatcgaaga tcacatccat gacttgtgca ggattcaatg
     3721 catttgcgat gaatacagct ggatcttcat tccattcgac gatatccatg ttttcgcctt
     3781 ttaattcatt gacgattgct tgaactcgtt gtccctttgg cccgacacat gtaccgactg
     3841 catcgatatt tggatcattt gagcgaactg ccacttttga acgatcgcca gcttcacggg
     3901 cgatgctgac aatttcaacg attccatcat agacttctgg tacttcttgt tcaaataagc
     3961 gacgcaatag gtctggatgg ctgcggctaa caaatacttg cggaccttta gacgtatttt
     4021 ctactcggga aacataaact ttgattcgat catgtggttg ataaaattca tttggcattt
     4081 gatcttgttt agaaagaact gcctcaattt taccaagatt gacataaata taacgtttat
     4141 cctggcgttc gacgattcct tgcatgatgt ctttttcata agcactgaat tcgttataaa
     4201 taatcgttct ttctgcttca cgtacacgct gtaaaataac ttgtttagcc gtttgcgcag
     4261 cgatccgtcc aaaatctttt ggtgttactt caaaacggat cgtatcaccg atctcatatg
     4321 ctggattgat taaaagtgca tctttcaatg agacttccaa ctgtgaatcc atcacttctt
     4381 ccgttacttc ttttaccgca tagacatgga tctttccttt tttctgatca aattccactt
     4441 cgacgttttg tgcttggcca tagtgacgtt tgtatgcaga aaccaatgct gcttctaaag
     4501 catcgatcac gatttctttt gagatccctt tttcagcttc taaagcgtct aacgcattta
     4561 acatttcttt actcattttt ttctgattcc tctccgtgat taaaattgga tagctaagcg
     4621 agcttttgca atattttttc ggtcaaaagt caattctttt tctcgtgttt ttatgcgtat
     4681 tttcaatgta agctgttcag catcaaatga ttgaagaaat ccttcatatt gtttttcgcc
     4741 atctactggt tggtataagg agacatggac ataatcacca agtgcctttt catagtcgct
     4801 ttcttttttc aatggccgct ctgctcccgg ggaggatacc tccaggaaat aagcttgagg
     4861 aatcggatct ggatctgctg catcaagttt ttcactcagt ttctcactga caaaggcgca
     4921 ttcttcgata tcgataccgc cttctttgtc aataaatact cgtaagaacc agctttttcc
     4981 ttcttttaca aattcgactt cgacaagttc aaaattttgt tcatctaaaa tcggtgtcac
     5041 catttctgtg actgtttcaa cgacgctact caaaagtttc gcctccttaa aaattaagtg
     5101 cgtaattcca ttaaaaagag tgagcgaaaa atccgctcac tcacagttag tatcattacc
     5161 tataagaact ataccatact ctgcttaaaa ttacaagcag acaggaaaac agatgcttat
     5221 agcatatcga ataacgataa ttgattttca tctggtagat ctttcaatac gccattgtcg
     5281 ttcatatatt cgatcaatgt ttttgaaact ttaccgcggg tggcaagatc ttctttggat
     5341 aaaaatggac cgtcttttct tgcttcaacg atttgtttcg ccacgttggt tcccaaactt
     5401 gggacagctc taaatggtgc gatcaacgta tcaccttcaa taacaaagtt aacggcatcc
     5461 gatttataca aatcaatcat tccaaatttg aatcctcgtt ccagcatttc attggctaac
     5521 tccagcactg tcagttgatt cttttctttt acggaagcat ctaatccctt gtccgtgatt
     5581 tctttcattg cttgtttcac agcatctttt cctttgcaca tggagactag atcaaagtcg
     5641 tctgcacgta cagaaaagta tgcacagtaa tacaaaatag ggaaataaac cttgaaataa
     5701 gcgacacgaa gagccatcaa tacgtaagct gcagcatggg ctttcggaaa catatatttg
     5761 atttttgaac aagaatcgat ataccagtcg ggcacattat tttctttcat cgccgataga
     5821 taggtttctc gcaattcatc agggatcttg ttccattgtc ctttacgaac agtttccatg
     5881 attttaaatg ccatcccgct atctaatcca gcatggatca aataaaccat aatatcatca
     5941 cgacagccga ttacttccgc cagtgtcgca tctcctcgtc gaatcaattc ttccgcattt
     6001 cctaaccata cgtctgtacc atgagatagt ccggaaattt gcagtagttc tgcaaaagtt
     6061 gtaggatgcg tttcttctaa catccctcga acaaaacgag tgccgaattc tggaatacct
     6121 aatgtccctg ttttggaata aatttgttcc tgacttactc caagtacttc aggaccggca
     6181 aatatccgca tcacttccgg gtcatccgta gggatcgttt gtgggtcgat ccctgataaa
     6241 tcttgaagca tccgaataac agttgggtca tcatgcccta gaatatctaa tttcaataca
     6301 ttatcgtgaa tcgagtggaa atcaaaatga gtcgttttcc attctgaatt ttgatcgtct
     6361 gctggatatt ggatcggcgt aaaatcatag acatccatat aatcgggaat aacgatgatc
     6421 ccccctggat gctggccggt tgtccgtttg actccagtag cgccttttgc taatctgtcg
     6481 acttctgcac ttcgaaactg cagattattg tccctttcgt atccttttac atagccatat
     6541 gctgttttgt cggcgactgt accaatcgtt ccggcacggt aaacatagtc ttcgccaaac
     6601 aatactttcg tatagttatg ggcttctgct tgataatcac ctgagaagtt caaatcaata
     6661 tcgggcactt tgtctccatg gaatcctaaa aatgtctcaa atggaatatc atgaccgtct
     6721 ttgtttaagc gtgcaccaca ttttggacat tgtttttctg gcatgtcaaa tccagaacca
     6781 tacgtaccat cttcaaaaaa ctcggaatat tggcactctg gacaataata atgaggggct
     6841 aatggattaa cttcagtgat tcctgtcatc gtcgcaacaa agctagagcc aacagagcca
     6901 cgtgaaccta ctagatagcc atcttcatta cttttatgca ctagtttttg ggcgatcaga
     6961 taaataaccg agaagccatt accattaata gaattcaact ctttttctaa tcgtttttga
     7021 atgatctcag gtaacggatc tccgtacatt tgttttgctt ttgtgtaact caattctgat
     7081 atttcatctt ctgaacctgg aatcttcggt gtataaagtt catctttgac tggaatcact
     7141 tcttcgcaga tatctgcgat tttattcgta ttttctacta cgatctcttt cgctgtttct
     7201 tcccctaaaa agtgaaatgc agttagcatc tcatcggttg ttcggaaatg gacatccggc
     7261 aaactatgac gattcaacgg attcgctccg cccatagaat tgatcaaaat ttttcggtaa
     7321 atggcatctt cttcgttcag ataatgtaca ttacctgttg caacaacgat tttatccaaa
     7381 ctttttccga tttctaccaa attttggata atttcttcca aatcatgttc attttttacc
     7441 aattcttgct cgatcaaagg cgcatagacc gcttttggca taacttcaat ataatcatag
     7501 aattttgctc ggtttcttgc ctcttctacc cctttttgca tcatggcttc gaatatttct
     7561 cccttatcac aagctgagcc gatcaataga ttttcacgca tctttttcaa ttgggagcgt
     7621 ggtattcgtg ggacgcgttc gaagtattcg acattcgaca ttgaaatcag tttgaacaaa
     7681 tctttcagtc ctgcctgatt ttttgctaaa atcgttacat ggaaaggtcg agcccttttg
     7741 taagaatctc cttctccgat atgctcattt agctgatcat gataaagcat gttatgattg
     7801 tccattgcct ctttcacaaa gatccatgct aagtgaccag ttgcttcagc atcatagatt
     7861 gctcggtgat gctgttccaa gctaacacca aactttttag ataacacacc taatccgaaa
     7921 cgtttgaatt gaggatacag atatctagct aattccaacg tatcaatgac tggattggcc
     7981 gcttcaggaa taccatacct tgcgtagcta gtattcaaga accccatatc aaaagccgcg
     8041 ttgtgggcta ccaaaattgt atcttttgaa aattcaagga acatccgcaa tacttcttct
     8101 tccgactttg accctcgaac catttcatcc gtgataccag tcagatctac cgttgttcgt
     8161 gacaatggat gtcctggatc gataaattca tcaaagcttt cgatcacatt tcctttatac
     8221 attttgactg cggctagctc gatgatcgtg tcatatactg cagacaatcc tgtcgtttcc
     8281 acgtcaaata cgacgtatgt ggcttcactc aatgcttcat gtgcatcatt ataagcaatt
     8341 gggacaccat catctacgac attcgcttcc acgccgtata agattttcac acctgctttt
     8401 tttccagcac tatgtgcttc cggaaaggct tgcgcaccac catggtctgt aatggcgata
     8461 gctcggtgtc cccattttcc tgcttgtgcc acaagatcag agatgctgtt tgttgcatcc
     8521 atcgtactca tattgctatg aacatgaagc tctactcgtt tttccccttc aggagcataa
     8581 tctttgcgag gtgtatgttt gacttctatg atatcttgcg cattcatgac caaatcacgg
     8641 acaaacgtat cttcttgaat acttcctcgt acctttaacc agctaccgac actgatcgca
     8701 tcaaatactt gttcatcttt ttcgccgttc gagaattttt tgacgataaa tgatgaagta
     8761 taatctgtga ttttcaaagt caagatcttg cgttttgaac gtagttctct gacttctttg
     8821 tcgaatacgt acccctcaag tgtgactctt cgttcttctt caacgatact gatcattggt
     8881 gtcgttggtt catcggctgg aatatttcgt cctagctgga tgggtccttc aagagctggc
     8941 gattgctctt ttctttcctt tttcttttgt tcgtggacaa ctagactttc agctgcttgc
     9001 ttcataaagg cttctgcctg ttcttgtttg cgttcttcaa aaagtttcaa tacacgttcc
     9061 gcttgctgct catccacttc tggttcgata cgaaattttg ggaatccgta actgacgaac
     9121 aactcttcta ctaatggcaa gtattgttgc ttcaaataag aaatagctcc tgtactgtcg
     9181 attggcaaga tgactttacg atcttttatc acaggtacct gcgttttcaa tacttgctgt
     9241 gctagtggtg tatcgcattg ctgattttcc aaagcttggg cccagtaatc ttgcaagagc
     9301 tgttcatcga atgtctggtc atctgctaag atattcagtt tgacttgtgc aatatcttta
     9361 aaagcgatag tcagatgctg agtcaatgac cgatatagca taataggcaa aattttggaa
     9421 aaatgtagcg taaattccca taaacgcgat ttacgatgga ccactacact atccatttca
     9481 ccagcagtaa gcagtggatg atttttttct gcttcctcta gttggatttg ttccaacagt
     9541 ttttcaaata aatctcgttt ttctgacaaa gatatacgcc ctttctgatg agaaacaatg
     9601 caattgatcg catcttatcc tcttcttttt cagtaccttt tagtatactc gttttttctg
     9661 agttttacat taaaaaattg gaactgcgat tcaaatcgca gttccaattt taaaagcgaa
     9721 tcatcagatt gtcaaaaaca gctgattcag ctatttttat tttctgtgtt ttaacgctta
     9781 ttattcgcct tggttcaaga ggattggtaa agtattcgcc aattcttctt tacgtacttc
     9841 aaccatttcg ccagttttct tgattttcac ttctacgatt ccatccactg cttttttacc
     9901 gacagtgatt ctgattggac agccaatcaa atcagaatcc gcgaatttca ctcctgcgcg
     9961 ttcatttctg tcatcaacca gtacttcata gccagctttt gtcatcgctt cttctagttc
    10021 attcgtcagg cttgtttggt attcgtcttt tagattcatt tggacgatat gcagatcaaa
    10081 tggcgcaatt ccttttggcc aattgattcc ttgttcatct gaatgttgtt ctacgattgc
    10141 tgataataaa cgacttacac cgataccata acagcccata atcacaaatt tttctcgtcc
    10201 attttcgtct agtacagttg ctcccataga ttcactgtaa cgagtgccga ttttgaatat
    10261 atgtccgatt tcgattcctc gagtaaaggc aagaacaccg ttgttgtctg gtgaaggatc
    10321 tccttcttgt acaaaacgaa gatcttcata agaaagcaca gtgaaatcgc gttttggatt
    10381 cacattttta tagtggaatc ctgtttcgtt cgctccagta atggcattcg ctaaatcttg
    10441 gacataacgg tctgcatata cttttacgtc atctgaaaca ccgactggtc cgacagaacc
    10501 aaactcagca cctagatatt tttgtgcttc ttcttctgtt gcttcttcta aaaagtcagc
    10561 agataaaaag tttttcagtt ttacgtcatt gacttcatgg tctccacgta ccaaaacaag
    10621 gacaggttgt tcatccgcaa tgaacaatac agacttgatg attttttgag gttcaacaga
    10681 aaagaaatct gctacttcgt caattgattt tacgttaggt gtagctactt tctccaattc
    10741 aagctgtgtt tcatgcgatt tttttgatac atagtagctt gtagccatct ccaagtttgc
    10801 agcataatca ctttcagtcg aataacagat cgtatcctcc ccgatttctg agatcgccat
    10861 gaattccttt gagtctttcc cgcccattgc gccgccgtct ccgatgatcg agcgaaattc
    10921 taagccacaa cgcttgaata cttcggtata ggctttttcg taatctttat atgtctcgtc
    10981 caaactttct tccgttgcat ggaaagaata tgcatctttc atgataaact ctcttccgcg
    11041 aagcaaaccg aagcgaggtc gtttttcgtc acgatattta ggttggattt gataaagatt
    11101 taaaggtaat ttcttatatg aattgatttc attacgcatc aaatcagtaa aagtttcttc
    11161 gtgagttggt ccaaggatca tttttcgatc attgcggtct ttgaaacgat acaagtttgg
    11221 tccatatgtt tcgtaacgcc cggattcttc ccaaagttct gctggcaaga tggcaggcat
    11281 cagcatttct accgctccga ttttttcaaa ttcttcccgc ataatgcgtt tcagtttttc
    11341 caatacacga tttgctaatg gcagatatga ataaataccc gctgatactt ggcggatata
    11401 tccagctctt agcaagattt gatggcttaa tacttctgca tcatttggaa cttcgcgtaa
    11461 tgttggaatc aacatttttg attgtctcat tctatatact cctttatttc ctcaaaatag
    11521 ttatcgtttg ttattatacc ctatttgtca actttttcta gaaaaagaat cgttgaatat
    11581 cgttccatgt aactagcacc atcaacaaca ttaaaaatcc aaagccagct aatgtcaata
    11641 ttccttcttt ttcttgactt aatggtttcc cgcgaatccc ttcaaaaata ttcaagacta
    11701 atttcccgcc atctaaggca ggaatcggaa gcagattgac gattccgaga ttcattgaaa
    11761 gaagcgccat caaactaata acagttgtta caccttgatt tgctgcttct gaagacaatt
    11821 ggtacatcat gacaggtccg cctaatttat ctaaactgaa acctgtaaat agtgatccta
    11881 aggctttgaa aatttccaat gaaccgctga aagcttgacg cgtccctcca atgatcttat
    11941 ccataaaacc tgtattcatt ggggctttta taccaagttg gccaactttt tcgccatttg
    12001 actctacaga tttcggtgtc acgtcgacag attgcacctg accttctcgt tcgatcttaa
    12061 agtctaatgt tttacctgga tttttcgtaa tgactgttgt cagatcattc catgaatgga
    12121 tttcttttcc gtccacactg actacctcat cattttcttt taagcctgct tctgccgcag
    12181 ctccattagg catgatctct cctacgcgat tagtgttcgt cacttgaaca ccaccttgca
    12241 taaatgccag aatgatgaat aacacgattg ccaaaataaa gttattcatg ggtccagcaa
    12301 agttggtcag catacgctgc cacaatttag ccgattggaa ctgtacgtct tttggcgcaa
    12361 ttctgatttc tgttccgtcg ttttcaataa tcgtcgcatc gtgatcaaca gggtaggtta
    12421 ctacttctgt ttcgtctcca ttcacataac cagtgatagt cagctcatct tctaaatcat
    12481 aacggctcaa ttccatcggg atagcatttg tcaattggat tttcttactt aaatttatct
    12541 tctctacgat gccgtcagaa tttagtaaaa gagaaagagg cattcctggt gccatttctg
    12601 tttcatcatc cccattgcct gccattcgaa cataaccgcc aataggtaat aggcgaagcg
    12661 tatatgttgt cccgtctttt gcttgatgcc cataaatttt cggacccata ccgatagcaa
    12721 attcgcggac gaggattcct gatcgtttcg caaagaagaa atgaccaaac tcatgaacaa
    12781 tcactaatat accaaaaacg atgataaatg tcagaatcgt tttcatagct tttttctcct
    12841 ttacttgttt tttgtgtttt ttataaaaca acgataaaac actataattt taccatagta
    12901 taccaaagac accaatagta tcaatataat gaaaaataaa aacctgcgtc ttttatgaaa
    12961 ttcacaaaga aacgcaggta tacttactaa ataatgccaa ataaatgcat gataggaaat
    13021 acaaacagca aactgtcgaa acggtctaag attccaccat ggcctggtaa gatatttccc
    13081 gagtctttca catcataatg acgtttgatt gcagattcga ccaaatcacc aaattgtccg
    13141 actgctgaaa aaataatcgt catcagaagc atgacaaaca taccatgatt gaatagttct
    13201 tttgctgggt atagcaataa ataaagaaat gcgactacaa cagcactacc gataccaccg
    13261 agcgcacctt caatcgtttt gttgggcgaa atatcaggac ttaacttatg ctgaccataa
    13321 cgtcgaccaa ccatatatgc tccgatatca gttgcccaaa caataaatag accaaatatc
    13381 aaaacagcca agctttctgt tcgtgcattg acaaaattct ggaaacctat tcccacatac
    13441 aagctgacaa gcacaggaaa acctgcttca tcaatcgtat acgtattttt agaaataact
    13501 gatgttccta aaagaatcat aactataaga taaaataaca taaatgtact tacattctct
    13561 ggaagaaaga aaaaccatct ttcttttggc aaaacaagta acactgcacc aacggcggat
    13621 aaaactcctt caaaacttaa gagtgtcaat cctttcatcc ggaacagctc gtaaactcct
    13681 actaccgcta agattgctgc tgtaagctca accgctatgc ctccgatcca tataatcgga
    13741 ataaacaaga ctagcgccac agctgctgtg atcacacgtt gtttcatgac tgatcccctt
    13801 tctcatcttc tgtttttttt acaccgccga atcgacggtc tcgattttga taagaggcga
    13861 ttgcttcttc aagatgcgca ccatcaaaat caggccaaag cgcttttgta aaatacagct
    13921 cactatatgc gatctgccat aacagaaaat tactaattcg ctcttctcca cttgtacgga
    13981 tcatcagctc aggatcttgt aactctttag gcaagaaacc tgtcattaaa tgcttagcaa
    14041 tcaattcatc atcgatttct tctgcgtgta tttctccttt tgctacttct tcggcaattt
    14101 cttggacagc agtcacgatc tctgctctac ttccataatt caaagcaaaa ttcaatacca
    14161 ttccagtatt gttttttgtt tgctcaattg cacgtcttac agcatcttgc gtatgttctg
    14221 gcaatacgtt ttcataaccc atgacatgaa ctttgacatt ttctttaatc aattctggta
    14281 cgaacgtatc aaagaagtcg acaggtagtt gcatgagaaa acttacttca tcttttggtc
    14341 gcttccagtt ttcagtcgaa aaggcataaa gcgtcaacac tttcactcca agccttgatg
    14401 ctttttttgt gactttcttt actgtttcca tgccttcttt atggccagca actcgtggca
    14461 aacgacggtt ttgcgcccaa cgaccattgc cgtccatgat gatggcaatg tgctgaggaa
    14521 ttggtccatc ggtattaaat tgaaattcgc ttttttcttg aatatatttg tttttttgcg
    14581 gaaaaaaacg taacattctt tttcctccta tacttaaaag attcccacta tcttaagtat
    14641 tatagcaata tctaactgtt tttcctatga aatcattgtt tttttattta aaaactgaat
    14701 aattttttct ttttttaatt tttaaataaa agaaatataa ataaaaacag aggaagcgga
    14761 atgatccgcc cctctgtttt tatgatttta ttaaacttcc aataattctt tttctttatc
    14821 agcagcgatc ttatctactt ctttaatgct gttatctgtc aatgtttgta cgtctttttc
    14881 taatccgcgc aaatcatctt cggtaatatc accatttttt tgttgttttt tatattcatc
    14941 gatcgcatca cgacggatgt tacgaatcgc gatttttgca ttttctgctt cttttttcac
    15001 atctttagct aattcttttc tgcgttcttc tgtcagttgc gggataacta agcgaatgac
    15061 atttccatca ttggttggac tgattccgat atcacttgcc ataatagctt tttcaacatc
    15121 ttgaagaatg ctcttgtcaa aaggtgtgat cattaaaaca cgtgcttctg gaatttggat
    15181 tgatgccatt tggttcaatg gtgttggcgc accgtagtag ttcacagtaa tacggtccaa
    15241 aaggctagcg ttcgcacggc ctgcgcggat ttgcccaagt tcacgttgaa gattttgtgc
    15301 tgcttttttc attttttctt ttgcttctgt cataattgca tctgccattt ttatttcccc
    15361 cttacagttg ttccgatatt ttcgcctaag attgcacggc gaatatttcc gtgttcattt
    15421 aaattgaaga caagcagtgg aatatcatta tccatgctta gtgaacttgc agttgaatcc
    15481 atgacttgta atcctttggc aataacttcc agatgagtca gttcttcaaa ttttactgca
    15541 tttgcatcta ctttcggatc agcagaataa acaccgtcta cattgttttt agccatcagg
    15601 ataacatctg cgccgatttc tgccgctcgt aatgctgctg ttgtgtctgt cgagaagtac
    15661 gggttgccgg taccgcctgc aaagataacg atccgtcctt tttctaaatg acgttctgct
    15721 ttacggcgaa tatatggctc tgcgatttga cgcatttcaa tcgaagtttg cacgcgtgtc
    15781 ggtacaccca cgttttccaa cgtatcctgt aaagctaagg cattcataac tgtagctaac
    15841 atacccatgt aatcagcttg agcacgttcc atgcccatat gtgctccgat ttgtccgcgc
    15901 cagatatttc cgccacccac aacgattgcc atttcaacgc ccagctcgtg aacttctttg
    15961 atttcttgga tgatttcttt gattactggt ggtttgattc caaatcctgc atccccagct
    16021 aaagcttctc cactcaattt taagactaca cgctgatatt tcggtgtgac catgttcatt
    16081 cctccaatat tttctattgc cattttacca tacttagatg atttcgacac tctaagactg
    16141 gtttttcttg ttttgcttaa aaaaagggag cgcacaaacc gtgcgttccc gtctgttatt
    16201 caacagaatt attttttcac ttggctcata acttcttcta caaagttatc ttcgcgtttt
    16261 tcgatacctt cgccaacttc gaaacgtacg aatgttttaa cagttgcgcc ttttgatgca
    16321 acgtattttt caacagtcat atcaggatct ttaacgaatg gttggtccac caaagcgatt
    16381 tctgctttga atttcttcaa gcgtccttct accatttttt caacgatatt cgctggtttg
    16441 ccttcgttca atgcttgttc tgtaagaaca gttttttcat gttctaattc agcttctgga
    16501 atttgtgatt cgtttacata acgaggattg atagctgcta cgtgcatagc tacgtctctt
    16561 gcaactgttt cgtcagttgt tccatctaga acagtcaata cggcaatacg tccgcccata
    16621 tgcaagtatc caccaaatgc agcattgtcg tctttttcta caacttcaaa gcgacggaag
    16681 ctgatttttt ctccgataac ttgagttgct tcgatcaagt cagattcaat tgtaccttta
    16741 tctgttttga ttttcattgc agcttccatg tctgctggtt tgttttcagc aacaagttct
    16801 gcaatttctt ttactaaatc ttggaacatt tcgtttttag aaacaaagtc tgtttctgag
    16861 tttacttcaa cgattgctgc agtgttacct ttcacagcaa ctgaagccaa tccttcagct
    16921 gcaatgcggt catttttctt cgcagccttt gccattcctt tttcacgtaa aagatcaact
    16981 gctttttcca tgtcgccttc tacttcaact aatgcttttt tcgcatccat cattcctacg
    17041 ccagtcattt cgcgtagttc ttttaccatt ttagctgtaa cgtctgccat gtttaactcc
    17101 tcctgtgtgt ttttcttttt aaaaaaagct gcttcaaagg agagtgaggc ttgtcgccca
    17161 gcctttgaaa cagctccatc attcaaaatt attctgctga agaattgtcg ccttcaacaa
    17221 catctacgat ttcttcgatt gatgttgctg tgttttcttc tgtgaaatct tcttcaacta
    17281 cttgatcttc accttggttt ccttcgatga aagcatcagc catttttgaa gtaatcaatt
    17341 taacggcacg aattgcatcg tcgtttgaag ggattactac gtcgatttca tctggatcgc
    17401 agtttgtatc aaccatagct acgattggga tatttaattt gtgtgcttct tgaacagcaa
    17461 tacgttcttt acgtgggtca acgatgtaca ttacatctgg gattcttggc atatcagcga
    17521 taccgcctaa gaatttttca agacgttcac gttctttgtt caaaccagca acttcttttt
    17581 taggtagaac ttcaaaagtt ccatcttctt ccattgcatt gatttgtttc aaacggctga
    17641 tacgtttttg gatagtatcc cagtttgtca atgttccgcc taaccaacga tggttaacga
    17701 agtattgtcc tgcacgagtt gcttcttctt tgattgcttc ttgtgcttgt ttttttgtac
    17761 ctacgaataa tgctacgccg ccttcttcag caacgctctt catgtaatca taagctgcat
    17821 ctactaattt aactgttttt tgcaagtcaa tgatgtagat tccgtttctt tctgtgaaga
    17881 tatatttctt catttttgga ttccagcgac gagtttggtg accaaagtgt acgccggctt
    17941 ctagtaattg tttcatagaa attactgcca tgtttgtttc ctccgatttg gttttatttt
    18001 tcctcttctt atcttctacc ttgccggcaa actttttcaa gcacctaccg acaatccaat
    18061 aagaatgtgg atttgctgat gcataaagca ccctcagcta ttatacagac aaaagatccg
    18121 tatcgcaaga gcaaatatac ttttctgctc aacattcaat agtttacctg aaagataaaa
    18181 agaattcaag aaaaacagta gatttctctt tgtttcttta ttatcctttt tccataaaat
    18241 aattttaggt ataaaagacg aatattattg ggcaatcctt cttttttttg ttatgataca
    18301 tgtgaaatct ttcgtaaagg agaaagaaaa tgcgcaaagc tcaaagacat ttgctgatta
    18361 aacaagtcat tgaagaattc ccgatccgta cgcaagaaga actattaaac aaacttgggc
    18421 attatggcgt taccgccacg caggcaacga tttctcgaga tatccgtgat ctaaaaatcg
    18481 tgaaggcacc tgatgaaaat ggcgtttccc gcttcgtatt gtttcgcgaa aaaggaactc
    18541 ctgagactag gactgaggaa gaggatcggc tggttcaaat gattgaagac attgtattga
    18601 aagtcgaacg agtccacttt ttgacgatcg tcaatacctt gccagacaat gctcatttat
    18661 ttgcatctgt tttagatgag atcaaacctc cctctattgt cagtacaatt gctggattcg
    18721 atacgatcat ggtcatctcg gaaaatgagg aggacgcaaa acgagtagaa gaatttcttc
    18781 accatccaac tgaagtatca cttttttaat cagagcacgt attcctaata tggaatgcgt
    18841 gtttttttat tttcaatatt ttccttatta aataaacctt tacactcttt acaaaaatac
    18901 atcatttttt attcaatttt ttcaacacct tgattgaatc acaataatca tcatttatat
    18961 tggatgttaa caagagcagc ttcttgctct ttgtgtattt caaaaaagaa atgaaaggaa
    19021 atcatatgta taaattatta actcctggac cattgacgac ttctgaaaca gtgaaaaatg
    19081 ctatgctgat cgatcattgt acttgggatc aagaatacaa agaaattaca caatggataa
    19141 gaaaaaaatt attggatctt gctcaagtat cggaaggaat atacacaacg gtcttaatgc
    19201 aaggtagcgg ctcctttggg gtagaatcgg tattaagttc tgtgatcagg ccagaagata
    19261 ctgtactgat ttgttcaaac ggcgcatatg gacaacgaat gatcaacatg tgcgaatgtc
    19321 tgaaactgaa ctataccgtt tatgaggtat cggaacatca aatcctccaa gctcatgtga
    19381 ttacacagct gattgaagag aatccctcta tctccgcact agcaatggtc catagcgaga
    19441 ctacttccgg tattttgaat aatttggaag agattgctgc tgtcacacaa gaaaaagatc
    19501 ttttgtttat agtggacgcg atgtcaagct ttggtgggat accgattcgt gtagccgaac
    19561 tagggattga ttttttgatt tccagctcaa acaaatgtat tcaaggtgtt ccaggctttt
    19621 cttttgtact tgctaataaa gcggaactag aaaagcgaaa aaatgtgagt cgaagtttgt
    19681 cacttgatct atatgaccag taccgcgtga tggaaaacga gaatggcaaa tggcgtttca
    19741 cttcccctac ccatacggtt ttggcttttc aacaagcgct gaaggaacta gaggcagaag
    19801 gaggaatcga aactcgatat tcacgttatc tcgccaataa tcatgtacta cgacaaaaaa
    19861 tgacagagat ggggttccag cctttcattc aagaatatca ggggccattc atcacctctt
    19921 ttctttaccc taaggactgg cgatttgatt ttaatcattt ttatcagtat ttaaagcaaa
    19981 acggatttgt gatctatccc gggaaaatca gcgatcatga atgttttcgt atcggaaata
    20041 ttggcgagat ctatccggaa gatattttgg aactgacagc aattatcaaa gactataagg
    20101 agaacgttaa tgattaaaac tgtaattttt gattgggcag gaacaactgt ggactttggc
    20161 tgtatggctc ctgtccacgc ttttagaaat gcttttctag aaaaaggaat ccaactgact
    20221 gacaaagaaa tccgcgaacc gatgggaaaa ttaaaatggg atcatatcca acagcttctt
    20281 tccctccctt ctgtcaaaaa acaatggata gctatttatg gacaactacc tcaggaaaaa
    20341 gaggtggagg aattgtatca acaatttgaa cattatctgt ttaaagattt ggtcaagcat
    20401 tctaccttga aacccgacac attggaaacg atcgaacggt tgagaaacgc tgggatcaat
    20461 attggttcca cgactggatt tactgctcct atgatgacaa tcgtagcaga aaccgcaaaa
    20521 aaagaagggt attcaccaga cttcatggca acttctgaag acgtcgaagg gtatggtcga
    20581 ccgtttcctt atatgatcca taaaaacatg cagtacttca aaaacaagtc ggttgatgaa
    20641 gtaatcaaag tcggagatac ggtctcagat atccaggaag ggaaaaatgc cggagtgttg
    20701 acagtaggtg taattgaagg tagttcgcta atggggattt cttatgatga gtaccaaaaa
    20761 ttagagatgg ggaaaaaaga tcgtttaaaa cagtctgttg ctcttagatt ccaacaagct
    20821 ggcgctgata tttgtgtaaa caatttaaaa gagttaacag aatacatttt gtacgctgac
    20881 aagaatccag aatgactttt atacaaaatc agaggtcgtg aaaaagctgt actgcatcaa
    20941 gtaataagaa cagccaaaca aaaatagctc ctcatatttt tcgtttggtt aggcttatta
    21001 ccgcagatgc aagcttttga acaccgttta ttcgaaaaaa aaagggagct gtgacacgac
    21061 ttttgtcaca gtccccttat ttacaaacaa acagtgttta agagacaacc tctccactaa
    21121 acggtgttca ttcagctgac tctcggtatt tgctcaaaaa acgataaaat ttgaaaagct
    21181 attttctctt ttgtttctct aatactcgga tcagaacgct gatttcacat tctctttttc
    21241 ttacatctta tctggtgcat gaagtcctag caaacgaagg tcttctttca ataatacagt
    21301 cactgcataa actagagcta agcgtgcttc tttttgttca tcatcagcta aaatcttcgt
    21361 atgagcatag tatttgttga atgcttgtgc taatttgatt gcatgtttcg caatcactga
    21421 cggttcgtat ttttccccag cagacaagac cgtttcaggg tatttttgaa ctaatttaac
    21481 cacttcccag ctggtatcgt cattcaatgc atagtctgct gtttctgaag ggacaaatcc
    21541 tgctttttcc aacaggctga ctgcacgagc atgtgtatat tgcacataag gtccagtttc
    21601 tccttcaaat cgaacaactt cttccaggtt aaagtcaaat gtattcagtc gatcgttttt
    21661 caaatcgtgg aaaatgactg ccccaacccc aacttgttta gctacagcat ctttgttttc
    21721 caaatcaggg tttttctcgc tgatttgttc tttagcagat tggattgctt catttaatac
    21781 ttcttctaat aagacgatct ttcctttacg agttgataat tttttaccac cttgcgtgat
    21841 caaaccaaat ggaatatggc gcatatcgtc agaccagtca aagcccattt ccttcaagac
    21901 agctttcaac tgtttaaaat gatagctttg ttcattccct actacatata gagattgttt
    21961 gaagtcgtaa gtacgtttac ggtacaatgc agcagctaag tcacgtgtaa tgtatagtgt
    22021 cgcaccgtct gacttcttaa tcaacgctgg attcaggtca taagcagata ggtcaacgat
    22081 ctctgcacct ttatcctcgt tcagcaaatg tttttcttct aaaagtttga cgatttcatc
    22141 catcttgtca ttgtagaaag cttcaccgtt caacgaatcg aaacgtactt ccagcagatc
    22201 atagatttta ttgaattctt tcatcgattc atcacggaac cattgccata attgaatagc
    22261 ctcttcatcg ccttcttcca gacgtttgaa ccaagcacga gcttcttcat ttaattctgg
    22321 ttctgtttca gctacttcat ggaattggac atataaacga agcaattcat tgattggttc
    22381 tgctttaaca gcttcttctg taccccattt tttgtaagcg acgatcagtt ttccaaactg
    22441 tgttccccag tcacctaaat ggttgatgcg gattggctga taaccgattt tttccataat
    22501 gaaaccgatc gaattaccga taactgtcga acgcaaatgt cccattgaga ttggtttggc
    22561 aatattagga gaagacatgt cgattggtac tgttccttga ttcccgatat tgctatcccc
    22621 ataatgttct ttttctttca ctactgtttg caaaactttt ttgctgataa gttctttatt
    22681 catgaaaaag ttcaagtaag gtccgacaac ttcgattttt tcaaagttgg cagaatcgat
    22741 tttttctgct aaatctgcag cgatttgctg tggcgctttt cggtaaactt ttgctaatga
    22801 aaaggctgga aatgcaacat ctccgtgttc agctgatttt ggattttcta gtaattgttc
    22861 gacttgttcc agtgttaaat catctttgac agcgtcatag acagcttttg cgacaagatc
    22921 tttattattc atctttattc ctcctaaagt gctcccttga ttgttaaaca aaaaaaatct
    22981 ctagcgacgg catacttagt atgccatcac tagagacgag atttcccgcg gtaccactcc
    23041 aattgtccta aaaaaggacc agctccatct gatatcggta gacgaccgtc tgtttaaaac
    23101 agaattctcc aagtgcgctt cgttgtatct tcttcattcg gcttccacca cttccgaatt
    23161 cgctagccaa gaatgataca agtactctct tgttcaacaa atcttagtct tattaaactt
    23221 tcttgaatta tatcagaaaa aaaaacgaaa ggctagtcgt ttctgataaa aaaacaatcc
    23281 agatggccta aaggagaaga aataccacct ggatagctat aaatcatttc gtaaatacga
    23341 aagacttaac aaattaatca gctacgataa tcgttccttc ttcagaagct aaaagattct
    23401 cgatattttc taaagaagtg atgattgcct tagcgtttgg ttttgcttca acaaatgcaa
    23461 tagctgcctc tactttcgga agcatgcttc ctggagcaaa ttgcttttca tcaatgtatt
    23521 gtttcatttc tgaaacagta acagtttcta atttcttttg atctggtttt tggtaattga
    23581 catacacatt gtctactcct gtcaatacga tcagcaaatc cgcatcgatg atttcggcta
    23641 atttttctga agcaaaatct ttatcgatta cggcttctcg tccttctaat ccagtagctg
    23701 tttctacaac aggaatccca ccgccaccaa cagagactgt gataactcct tgatcgacca
    23761 gcgtttcgat gacacgtgct tctttaatcg agatgggttt aggggaagcg actacttttc
    23821 tccatccacg tccagcatct tctttgaatg tcacagtgga gtcagcattc atttgttctt
    23881 tggcttcttc ttccgtgtag aatggaccga ctggtttact tggatttttg aatgaaggat
    23941 cattttcatc cacaataact tgagtgacta aagaaaccac atctttatcg atcccttttt
    24001 ccttcagtac ttcgcccatg gcattttgta accagtaacc gatagatcct tctgtcattg
    24061 caacacatgt atctaaaggc attgccggtg ttttctcaga atcagcagct tgctgctgaa
    24121 tcaataggtt accgacttgc ggaccatttc cgtgggagat gatcaattca tctccttgtt
    24181 cgataaattt cactaaatac tttgctgttt ccattaacgc ttcttgttgg gcttttgcac
    24241 ttgcatccgt agacaagatt gcattacccc ctaaagcgac aactactttt cgtttggcca
    24301 tttcattcgt cctttcttca gttctaccga attttctaga taacaaaacg agccagcgtt
    24361 ttgtgatttt ccctcaatgc tgactcgttt tttcgtgaga tgaatgtggt tatgcttaaa
    24421 cgcgagggat aaacaagtta cctaatgttg ctgccatgat cgctttgatt gagtgcatac
    24481 gattttctgc ttggtcaaat tgacgagcat atttgctgcg gaagacttca tctgttactt
    24541 ccatttcagt gatgccaaag cgttctttca tttgttcacc gtaaacagtg tttgtatcat
    24601 ggaaagctgg caagcagtgc aagaagatca aacgatctgt gttgtgtgtt ttttctacca
    24661 tttccatatt gatttggtaa ggtttcaaca atttgatacg ttcttcgaat ttatcttctt
    24721 cacccattga tacccataca tcagaataaa ggacgtctgc tccgtctaca cctttatcaa
    24781 catcgtcggt aatcatcaat tgtgcaccgg atttttcagc aaagccttct gccattttga
    24841 cgatttcttc ttcaggttgt aattcttttg gtgcaacgat acgcatattc gctcctaaaa
    24901 ttgcacctgt gactaataaa gagttagcca tattgtttcg accatctccg cagtaagcaa
    24961 cagtgatacc ttctactgtt ccaaagtttt cttggatagt taaaaagtca gcgatcattt
    25021 gtgttggatg ccattcatca gtcaagccat tccaaactgg aacaccagag aattctgcta
    25081 gttcctctac cattttttga ctgaatccac ggaattcgat tccatcaaac atacgaccta
    25141 gtactttagc tgtatcttct gtggattctt ttttacctaa ttggatatca tttgcaccta
    25201 gatattcagg atgtgcacct agatcgatcg ctgcagtagt aaatgcagaa cgagtacgtg
    25261 tagatgtttt ttcaaataaa agcgcaatat tttttccttc tagatagtga tgtgggatac
    25321 cacgtttttt caagtctttc aagtgttctg aaaaatcgat caaatattgt aattcttctt
    25381 ttgtaaagtc tttttctgct aataagcttc ttccttggaa tactgattct ttcatattaa
    25441 acattctcct ttagattgtt tttatttaag attaaagagg aaatcccgac ggcagctgac
    25501 agtaagaccg ccgtcaggaa acaaattact tcaagtcttc acggacaagt ggttggctca
    25561 tgcagcgtgg accaccacgg cctcgtgaca attcacttga attgatttct aacactttga
    25621 tgccatagct acgaagcaat tcgttcgata cataatttcg gttataagtg actacaacac
    25681 ctggagcgat tgccaatgtg tttgatccat cattccattg ttctcgaggt gcaacgatct
    25741 catctccatt accagttggg atcagtacaa gatcatctaa tccaagagca gctttcatcg
    25801 tttcgtgtaa gttatctgaa tgagtaatct tgatatcatc accatctggt tcgattgtaa
    25861 agacatccat cttaccgtct ttactttgaa ttgctggatg gatagtgaat ttatcgtgat
    25921 ctaccattgt aaatacggta tccaaatgca tcattgcacg gttgtttgga attttgattg
    25981 ctaacacttt ttcaaatcct gaattttttg cgaataggtg gcgtgctaat ttttctaaag
    26041 ctttcgcatt agttcgttga gaaatcccaa cagctactac tttatcgctt aagatcaatt
    26101 cgtctccgcc ttcgatatga tctggatttt cacgatccaa ccatacttcg attcctttat
    26161 ttgcaaatct tggatgatgt ttcaaaatat attctgtaaa catcgattca cgacgacgtg
    26221 cttcaaaagt catcttattg actgtcatcc catttcccat tgatgcggaa gggtcgcgtg
    26281 taaagtacaa gtttggcatt gggtccatgt aaaatggata gtcatcgtct gctgacagat
    26341 cgtatagact gctagaacgt acttcaatat cttttgtacg aaccccagcc atgattttat
    26401 ctaccatttc ttgcgtttcc attgatagaa ggaactcatg aagaccctct cttacagcat
    26461 tcgatgcaat atgagattca tccaacattt tattcaagaa ttgttctttg acattgcctg
    26521 cgtcaatcgc ttctgccgct aatttttcca agtaaagagt ctcgacgcct tcattttgca
    26581 atgtctttgc aaagttgtca tgttcttctt gagcaatcgg aagatatgga atatcatcga
    26641 acaacagacg atccataata tctggtgtca aattttccac ttcttgtcca ggccttttca
    26701 gtaaaactgt ctttaatttc cctatttcag agaaaacgtg aataggttta tccatgttgt
    26761 gttgcctcct tctttttgat tacatgtata ggatatcctc cttttgtaat cactttcata
    26821 attttgtctc attcaaaatg agacattcct cacattaata ttcgataatt aaataattaa
    26881 acgtttttct atttttaagc g
//
LOCUS       DACDEG010000027        25690 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.027, whole genome shotgun sequence.
ACCESSION   DACDEG010000027 DACDEG010000000
VERSION     DACDEG010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.027"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            122..3649
                     /locus_tag="IV142_001239"
     CDS             122..3649
                     /locus_tag="IV142_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP8484908.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3646..7368
                     /gene="addA"
                     /locus_tag="IV142_001240"
     CDS             3646..7368
                     /gene="addA"
                     /locus_tag="IV142_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP8484909.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7437..7778
                     /locus_tag="IV142_001241"
     CDS             7437..7778
                     /locus_tag="IV142_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8484910.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8087..9130
                     /gene="pheS"
                     /locus_tag="IV142_001242"
     CDS             8087..9130
                     /gene="pheS"
                     /locus_tag="IV142_001242"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP8484911.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9135..11555
                     /locus_tag="IV142_001243"
     CDS             9135..11555
                     /locus_tag="IV142_001243"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP8484912.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11725..12735)
                     /gene="trpS"
                     /locus_tag="IV142_001244"
     CDS             complement(11725..12735)
                     /gene="trpS"
                     /locus_tag="IV142_001244"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP8484913.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12989..13726
                     /locus_tag="IV142_001245"
     CDS             12989..13726
                     /locus_tag="IV142_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8484914.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13740..14555
                     /locus_tag="IV142_001246"
     CDS             13740..14555
                     /locus_tag="IV142_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP8484915.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14568..15215
                     /locus_tag="IV142_001247"
     CDS             14568..15215
                     /locus_tag="IV142_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8484916.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15231..15887
                     /locus_tag="IV142_001248"
     CDS             15231..15887
                     /locus_tag="IV142_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8484917.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16049..16870
                     /gene="racE"
                     /locus_tag="IV142_001249"
     CDS             16049..16870
                     /gene="racE"
                     /locus_tag="IV142_001249"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP8484918.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16873..18228
                     /gene="rph"
                     /locus_tag="IV142_001250"
     CDS             16873..18228
                     /gene="rph"
                     /locus_tag="IV142_001250"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP8484919.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18229..18747
                     /locus_tag="IV142_001251"
     CDS             18229..18747
                     /locus_tag="IV142_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP8484920.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18854..19354
                     /locus_tag="IV142_001252"
     CDS             18854..19354
                     /locus_tag="IV142_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8484921.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19634..20554)
                     /locus_tag="IV142_001253"
     CDS             complement(19634..20554)
                     /locus_tag="IV142_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP8484922.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20719..21732
                     /locus_tag="IV142_001254"
     CDS             20719..21732
                     /locus_tag="IV142_001254"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP8484923.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21842..22597)
                     /gene="fabI"
                     /locus_tag="IV142_001255"
     CDS             complement(21842..22597)
                     /gene="fabI"
                     /locus_tag="IV142_001255"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP8484924.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22817..23257
                     /gene="fabZ"
                     /locus_tag="IV142_001256"
     CDS             22817..23257
                     /gene="fabZ"
                     /locus_tag="IV142_001256"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP8484925.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23372..24133)
                     /locus_tag="IV142_001257"
     CDS             complement(23372..24133)
                     /locus_tag="IV142_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8484926.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24322..25416
                     /locus_tag="IV142_001258"
     CDS             24322..25416
                     /locus_tag="IV142_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP8484927.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(25502..>25690)
                     /locus_tag="IV142_001259"
     CDS             complement(25502..>25690)
                     /locus_tag="IV142_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8484928.1"
                     /translation="NSNGILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
BASE COUNT         8508 a         4454 c         5415 g         7313 t
ORIGIN      
        1 acttaaaaaa gtagaaatat cagaacctta aaaggatata atttgaagaa tatagaacaa
       61 atgttcagta ttttttgata taataaagga gcaccgtatc atgtgttgaa ggaggaaaag
      121 catgtcttta caatttattc ttggaaacgg gaaaaaagat caccgtcaag tcttgatcga
      181 cgaagcatcc gcttggctcg accaggatgc aagtaaccaa gtatttttcc ttgttcccaa
      241 ctacagcaaa ttcgaacagg aacaagaaat tttagcggag atgcgaagaa gaagcggaaa
      301 aaaaactttc agcacaacaa acgttcaagt atttagtttt tatcgtctgg cttggtattt
      361 gctgcaacag acaactcttc ttacaggaaa tgaattatca gaatcaggat ctgcaatgat
      421 cttgcgacag attttagaaa aaaatgagga agaattaaca atctttcgcg gagaaatcaa
      481 taaaagaggg tttattcgtc aattacaaga actctacagt gaattgcgta caggaaatat
      541 tcaaccagaa gacctgatgc ttttatttgg tgattcacct aatgcagaag accagcaact
      601 taaaatgaaa gacttgaagc tgattttttc agcttatgac agcgaattga ctcaacgtgc
      661 actaacaaat gaagatgcac tatctatact agcaaattat atgcagacac aagatttcaa
      721 caacgtcaaa tttattgtca gtggtttttc tcgaatcaat ggtcaagagt atcaattact
      781 gcaggcaatg atggaaaagg ggcaattggt tgtcgattta ttgctggatc gtccttatac
      841 agcggatctt ccagaagtac ttacgctctt tcatgagact ggaaagcttt atttccgtct
      901 ttttcaaagt gcaagagaac ggaaagtccc tatattagtg gaccgatttg caccagagca
      961 gactcgtccg acagagcttc aactgctgca aacaatgtgg gaagagacat ctagccaaaa
     1021 gaaagcggca gtacacaaaa tagggggaca agagcatctc catttgtggg aagctgaaaa
     1081 tccaactgaa gaagtccgac aattagctgt tgaaatcaga cgactagtca gtgaagaaaa
     1141 ctaccgttat cgtgatatcc aagtgctgac aaaaaatatg tctttatatg gaaatatcct
     1201 tcggccgatc tttcaagaaa tggcaattcc tttttatatc gatgaagaac aaatgatgga
     1261 aaatcatccg ctgatcgaat ggatcaacag tctgttcgct cttgaccgct attcttatcg
     1321 gctaagtgat attatgcgtt ttttcaaaac tgaattattt ctgtcgacgg aagcagaaac
     1381 cgaacttgat gaatggaaac aagagcgaga tgaattcagg aaaaaactgg acatcactga
     1441 aaatgtagca cttgcttaca attatcaagg aacttattgg actcgtgaaa aagactggca
     1501 gtttgtggcg tatgattttg aagcagaaca gattgaagac gtacagtatt tggaagaaca
     1561 gtcaaatacg attagacgat tatttcagaa agctgtacca gtatttttga aaaaaataaa
     1621 gaaaagcaaa acaggacaag aggccgcaaa tgttttttat caatttttga tccaaagtgg
     1681 cgtacagcaa caattattgt tttggcgtaa tcaggaagta gacagaggaa acttagaagc
     1741 ggcaagaaac catgagcaga catggggagc gctgattgat ctgttggatg aatatgtagt
     1801 aatttatggt gaatcggctt ttgactggtc actgttccaa gaaatcatta caagtggtct
     1861 agaaaatcta agttatggta aaatccctac cgcaatcgac caagtgcgaa tcaatcgttt
     1921 ggaattagtt cgaccaaatc aagctgccgt cacttttgca ttagggttga atgatcaagt
     1981 gtttccagac cgaaaagaga caaaaggatt gttgtccagt gaagaacgag aagtattaaa
     2041 tcaggccttg ccagatggac agtttttatt cgacccatca aaggaaagta tctcttttga
     2101 gcctttccaa gcctatcttg tttttctttc cggaacagat cgattatatc ttagttatgc
     2161 acaaagctat gacacagatg gttcgctgaa gatgtctcct tatttgcgta gaatcacaga
     2221 atatttatct gttcccgtcg agagaaaaga gcatttgacg atcgaaagcg accctaattg
     2281 ttatgtaggg acttacagaa gcggaatcaa tacagtcaat aggatttatc gattagcttt
     2341 agatgaaaaa aaacctgtac caaagcactg gcaaaagctc aaagaattag tactgaattc
     2401 gtcatggcga aattttgctt tacagatttt tgaaagtcag gaacatttaa atgttcctgt
     2461 ttcgatgcca gaaaaaatgg cggaagagct ttatggaaaa gagatttata cttcaatttc
     2521 acgtatggag aatttttata attgtgaata caaatacttc atgcaatttg gtttgaaatt
     2581 gaaagaacga actgtttatg gtctaactcc agcagcaact ggtgattttt atcatgaatc
     2641 attggatcgt ttcttcaagc tactgttttc aaaccaatta tccttagtga atatgtccga
     2701 tgcgcagcgt agagacttta ctgaaaaagt gctgcaggaa gtattcggag aacttcggtt
     2761 tgagatattg gatagttctg caagaatgaa ctatatccgc taccagttag gacagacgat
     2821 tcaaaaagtg acatgggctt tgcaaaaaca gagcaaaaat acaggcatgc atcctcttca
     2881 gacagagatt ctatttggtc agattgctgg agcaaaaggt atcacaggtc tggaattacc
     2941 gttgagtaat ggtggaaaac tccatgttcg agggaaaatc gatcgaatcg atgtagcttc
     3001 tgaacaagaa gatacttggc taagcgtcgt tgattacaaa tctagcggac gttcattcga
     3061 tgtcacagaa gcttattatg gcatggctat gcaattattg acgtatctgg acgtagcatt
     3121 gatggatgcg gtacgtttga ctggaaaggc ggctgttaaa ccagcaggat catattattt
     3181 acatgttcat aatcctgtat tgacggagac agatgatgta gagaagaata cattgaaaaa
     3241 atttagttat gatggcgttt ttgtcgatga tcccgctctt ttagaagtac tagatcattc
     3301 actggaagca aaagaacaat cactgatcta tccagtgaaa aaaaatgcga aagatgaata
     3361 ccaaaaaggt agtttcagta aagacaaatt tttcacggaa gaagaactgc aactattcat
     3421 ggcgcataat cgggaaaata tgcggttagc tggagatgcg attacttccg gagaaatcaa
     3481 actaaatcca gcttataaag ataaacagcg gattgcctgc gaattttgtc cattcagaag
     3541 catctgtacg tttgatgtga tgctgaaaga aaataattac caccgtctgg agaaaataga
     3601 taaggaagaa gcactgcgta gaatattgaa aaggagtgaa gaagagtgaa tcaaattcct
     3661 ttaaaaccgc aaaatgaacg attcacagat gatcagtggc aagcgatttt tgatcaagga
     3721 gataatctat tagtctctgc gtctgccgga tcaggtaaaa ctacagtgct tgttcgaaga
     3781 gtgattgaaa aactaaaaat gggttttgat atcgatgaat tattgattgt gacatttact
     3841 gaagctgccg caagagaaat gaaagaaaga attcaagagg cattacaaga atccgtcaat
     3901 agcgaaagtg accctgtacg tagacagcat tttactaagc agttagtttt attgcccaca
     3961 gcgaatatct caaccttaca tgctttttgc ctgacagtta ttcgacgtta ttattactta
     4021 attgatattg atccagtctt tcgcatgtta acagatgaaa cagaaacgat cttgatgaag
     4081 gaggatgtgt gggacgagct gagagaagca ctttatgcag aaaatgatga acgatttttc
     4141 caattaacaa tgaatttttc taatgatcgt tcagatgacg gcttgacgaa tctggtgttt
     4201 tctctttatg agtttgcccg tgccaaccct gacccccaaa aatggctaga acagttaagc
     4261 gataattatc gtttgcctga ggggctagcg aaatctcgat tatatcaaaa acaaatccgt
     4321 cctttagttt tggcagatat ataccaatgt gtacagttat atgaacagat gactcagtta
     4381 gcacaaggtg aaggattaga aaaaatgcat gagcaagtag ctggagaaca acagcagatc
     4441 aagaatatct atgaagcgtt ctcgcaagat cggttagaag aagcttatgc tggtttggaa
     4501 caactaactt tttcaacttt taagagcagt cggaaagcag aattgaaaga aatttccaat
     4561 gaagtcaagg gcatgaggga taaagcaaaa aaactgattc agcagataag taaaagttat
     4621 tttcctgttt ccccaagcca aatggaagaa ttaacggaca aagcactacc gttagtcgaa
     4681 gaaatgacaa aagtcacgca aagcttcatg gacggtttca gtatgcgtaa acgggaaaaa
     4741 ggtgtgctgg atttcaatga tctggaacat ttagcgttgc agattctcac agaaaaaacg
     4801 aaagatgtat ggctgccaag tgaagcttca aaacattatc gaaaaaaatt caaagaagta
     4861 atggtagatg aatatcaaga cgtgaaccaa ttacaagaag ccattttata ctggctaaga
     4921 gaacccgatg atacaaaagg aaatatgttc atggttgggg atgtcaaaca atccatctat
     4981 tctttccgtt tagcagatcc tagtttattt atcgggaaat acgagaattt ctcaaagaaa
     5041 gaaggcggac gtcgaatcgt tttggctgag aacttccgct ctagaaaaga agtcctttct
     5101 tttacgaatc ttatttttga acaactaatg gatccagcag tgggtcaaat caattatgac
     5161 gaagcagcca aactgatcca aggattttca gactttccag agaatgaaca gtttgagccg
     5221 gaaatcatga tctatgaaaa agaacaggaa gaatcagaga ttgagattcc gacggatgat
     5281 atactggaag acaaaacaga aggcgaactt tttatgacag gactaaaaat tcgtcaatta
     5341 attgattcct cctttatgat ttatgataaa aaatcaaaaa aaagtcgtcc gatagagtac
     5401 aaagacattg ttctcttgac acctacaaag aagaacaact taacgatttt agagattttc
     5461 aaaacattgg atattccatt agagatgaat gatgctcaaa actattttca agcgacagaa
     5521 attcgcacga tgatctcgct gctacaatta atcgataatc cctatcaaga tattccgctt
     5581 gcggctgtat tgcgctcacc gatcgtgggt ctgattgaac cagaattagc cagcattcga
     5641 ttagctaacc gtgcacatac ttactatgat gctgtgcttg cttatcaagc atcaaacgaa
     5701 gatgaattag cagctaagct ggaacatttt ggcaagcaac tagagcattg gcgagaactt
     5761 gctcgccgtt cttcgatcac tgacttgtta tgggatatct attatgagac gggatatcta
     5821 gagtatgtgg tcggtctgcc agcaggggca cagagacaag ccaatcttta tgcattggtt
     5881 gatcgtgcca aagcttacga gcaaagttct ttcagaggat tataccagtt cgtgcgtttc
     5941 attgaaaaaa tgcaagaaaa agataaagac ttagcagaac cagtcatttc aatagaagat
     6001 aatgctgttc gcgttatgac gatccatgca agtaaaggct tggaattccc agttgtattt
     6061 ttgttagata tgacaaagga gttcaactta caagacttgc gcaatcggta tgcttttgaa
     6121 gaaaaacttg gagcaggaat tcgctatatg gatcctgaaa cacgagtgtt atatgatacg
     6181 ttaccgtttc aagccatcaa attggcaaag caaaacaaat tgttgtcgga agaaatgcgt
     6241 aaattgtatg taggattgac tcgggcagaa caaaagcttt tcattgtcgg ttcctataaa
     6301 aataaagagc aaatgatcca aacgtggtct gaagcagctg atcatgagga gttagttttt
     6361 gatcctgcgc tacgtttgaa gggcagaagc agcttgatga attggattgg ttacggattg
     6421 atccgtcatc ctgaaatgca gaaatactta gaggaagaaa tttcgacttc tcttcttcaa
     6481 cattcaaatg cacagttttc tatttcatgg atgaatcagc aatcgatcat tgaacaaaga
     6541 cagttgcttg ctgaaaaaga actggttaac ttagatcagc agatgaaaga agatgaaact
     6601 ttattagcag attcgttaca aaaacgtctg gcatatgaat atccttatca agcttcttca
     6661 caaaccacaa gttatcaatc tgtatctgaa atcaaacgat tattcgaaga tccagatgat
     6721 acacaagaat cacgtttgac tctggaaagc agtcagaaca aagcagcatc ccgtcaattt
     6781 cgttatacgc aagaacaact agcagaacca aaatttttgc aaaaagatcg gcaagtgtca
     6841 gcagctgctg ttggaactgc tacgcatgct ttgttacaat tactgccatt agagatgcca
     6901 actacagaga gtatccatca aaaattacag gaattggtaa ggaaacggtt agtagacgaa
     6961 aaagtcgcga aaaaagtaga tgtttcttct attatatggt tctttcaaac agaacttggt
     7021 caacagctga ttgccaacaa agaaaacgta aaacgagaac aacctttttc catgctgctt
     7081 cctgctgacg aagtatttca agactatcca aatcaagaag atgaactatt aatccacgga
     7141 atcgtcgatg gatacctaga agaaaaagat catctcaata tctatgattt taaaacagat
     7201 tttattttac atccggatga tccagcagaa atagatgcaa tcgtccaaaa atatcaagga
     7261 cagctaagac tttaccaaca agcaatgtca gaagctttga ataagccagt agaaaatgtt
     7321 ttcctaattt tgcttcgagt gaaacaaatt ataaacatta acaaataatt ataattgagc
     7381 aaacttgact tatgttttgt aaggattatg ctatactctg ggaagaggtg agtatgatgg
     7441 agctaacaca gacagaattc gttttatgcc ctaaatttga aaaaacattc tctattttag
     7501 gaaaaaaatg gaacggattg attatcgatg tactactcga aaacggtccg caacgctttg
     7561 ttgagcttgc ggcaaaaatc ccgatggtca gtgatcgagt attagtcgaa cgcttgaagg
     7621 aattagaaaa agaaggaata gtcactcgaa actttgcctg tagcgaaaag agccgttcag
     7681 aatatctctt gactgaaaaa ggagcagact tgcaaaaagc gatgcgtcaa atacaagttt
     7741 ggagcgaaaa atggatcaca gaggaagaat gcggttgatt ttgtaaaaaa aatcttgtaa
     7801 actatagtcc atacgaataa gtaaagaacg atgacgaaaa agagtagttt ggctaaacca
     7861 gttcagggag atctgtcgca gactgaaagc agatcctgtg tttgaaaaat gaagttcact
     7921 ttccgagttc gtctgataaa atcagaccgg caattgtcgt taacgaagta agagtgcggt
     7981 gtaagtcatt tatccgcaaa ttaggatggt aacgcgaaga cctcgttcct taacaggaat
     8041 gaggtctttt tgttttgtat ttggaattta aaggaggaac aagcacatgt ctctacaagc
     8101 acaattagag agcttgaaag aagcaacgct cgtcaaaatc acagcggcac aagatttaaa
     8161 tgaattaaat caaatacgtg ttgaaacgtt aggaaaaaaa ggaccgatca cagaagtatt
     8221 acgtgggatg aaagatctaa gtccagaaga gcgaccaaaa gtcggaagtt tcgcaaatga
     8281 gatccgagat cttttgacag aagagatcga gcgaaaaaaa caagtattag aagaagaagc
     8341 gttaaatgca gctttggcaa aagaaacaat agacgtgaca ctacctggta gaaaagtaaa
     8401 acatggtaca cgtcatatcg tgacacaaat catggaagaa attgaagata tttttgtggg
     8461 tatgggctat cagatcgtag aaggatatga agtagaatct gatcattaca atttcgaacg
     8521 gatgaatctg ccaaaagatc atccagcaag ggacatgcaa gatactttct atatttcaga
     8581 cgagatattg attcggacac atacttcacc agtccaagca cggacaatgg aaaaacatga
     8641 tttctctaag ggtgctctac gaatgatctc gccagggaaa gtcttccgca gagatacaga
     8701 tgatgcgacc cacagtcatc agttccatca aattgaaggg ctagttgttg ataaaaacat
     8761 cacaatgggc gaccttaaag gaacattaga agtcgtaatg aaaaaaatgt ttggggaaga
     8821 tcgtgaaatt cgtttgcgtc caagttattt cccatttacg gaaccatcag tagaagtaga
     8881 tgtcagctgt ttcaaatgtg gtggtgccgg ttgtaacgta tgtaaataca ctggatggat
     8941 cgaaatttta ggagctggca tggtacaccc gaatgtgttg aagatgtcag gaatcgatcc
     9001 agaagaatat tcaggctttg ctttcggcct tggaccagac cgcgtagcta tgttgcggta
     9061 tggagtgaat gatatccgta atttctatca aaatgactta cgtttcttaa gtcagttcaa
     9121 gggggagtaa gaaaatgtta gtttcatata aatggctcaa tcgttatgta gatttatcga
     9181 atgtaacacc aaaagaatta gcggataaga tgtctgtgac aggaatcgaa gtagagggag
     9241 taacggttcc agaagaaggc ttgaaaaaaa tcgttgtcgg tgaagtcaag gaatgcgtac
     9301 cacatccaga ttctgatcac ctttctattt gtcaagtcga tattggcgaa gacgaactgt
     9361 ctcaaatcgt ttgtggagcg cctaatgtga aggttggaat caaagtgatt gttgcacttc
     9421 ctaactcacg tatcgctggt aatgtaaaaa tcaaaaaagg aaaaatgcgt ggccaagtat
     9481 caaatggcat gatttgctca ttacaagaaa ttggctattc agatagtgtt ataccgaaag
     9541 aatacgctga aggtatctat tatatgccac aagaagctgt aaatggcgat ccagtttttc
     9601 cttatttgga tatggatgat tcgattattg aattatctat cacaccgaat cgtgcggatg
     9661 cattgtcaat gcgtggcgta gcttatgaag ttggggcaat ttatcgtcag actcctacat
     9721 tcgatgacga aaagctggta gaatcctcac gtcctgctag cgataaaatt caagtggaag
     9781 ttgaagataa agaagcagtt cctgcttatc aaatcagaat cattgaagga gtaagaatcc
     9841 agccaagtcc tcaatggctg caaaatcttt tgatgaacga aggcattcgt ccaatcaaca
     9901 atgtagtaga tgtgacgaac tatattttgc ttttattcgg acaaccacta catgcatttg
     9961 actacgacaa attgaaatca aaagagatct ttgtccgccg tgcaaaagaa aatgaaacat
    10021 tagtaacact agatggagaa tctcgggaat tgacaactga caatctcgtc attactaacg
    10081 gcaaagaacc agttgcttta gctggtgtca tgggagggtt agactcagag atcacgaatg
    10141 aaacaacaac agttgcacta gaatctgctt tatttgatcc aatttcgatt cgtcgtacag
    10201 caaaacaatt taatttgcgt agtgaatcct ctgcacgttt tgaaaaagga atcaaccatg
    10261 cgactgttgg agaagccagt gaggttgctg cagcaatgat cgtttctctt gcgggtgggg
    10321 aagttctaca aggcgcggtc aaaggctcag agatcaaagc agaagacgta tcagtgaaga
    10381 tttcacttga acgcatcaat caatatttag gaacatctct tgtcgttaaa gaagtaaatg
    10441 agatttttga agcacttgga tttagctatg aagaaaagga gtatgtttat tccgtagtag
    10501 taccaccaag acgctgggat atccaaatcg aagcagatat tatcgaagaa gttgctcgta
    10561 tttatggata cgaccagttg ccatctacac ttccaagtgg agaaacggtt gctggaagct
    10621 tgtcaaaaga acaagaaact actcgtaaaa ttcgtacaat ctttgaagga agcggattga
    10681 gcgaagcaat cagttatgca ttgacaacag aagaaaaatc aagacagttt gttataaatg
    10741 aaagtaatat tacacgtttg gactggccaa tgagcgaaga acgttctgta ttacgaatga
    10801 atttgatcag cggactatta gatgatatca gttataacac tgcccgaaaa aatacagaga
    10861 ttgctttata tgaaattggt cgggtgttct tccaagaaaa tgatccacgt acacatctgc
    10921 cgcaagaagt aaaacatgca gcaatcgcta tttctggtat ttgggaagaa aaagattggc
    10981 agacgaaagc tcaaaatgta gactttttca ccatcaaggg tcttttagaa aacttatttg
    11041 aacaactagg catcactgaa gatgtccatt atcaaacagt gacagaaatg aaggaaatgc
    11101 atcctggaag aactgctgcg atttaccttg gcgaaaaatt tattggtttt gtcggtcaag
    11161 tgcatccaac gattacaaaa agttatgata ttccagaaac atatgttgct gagtttgatt
    11221 tatctgctat agtggaagct gctcaaaaag ggatcgtttt cgaagcagtg acaaaattcc
    11281 cagcagtcag ccgggacatc gctttgcttg taaaagaaac tgtgacaaat caggaattaa
    11341 cagaagttat ccgttctgct gccggaagat ttttaacaga tatccaatta tttgatgtct
    11401 atcaaggaga aaacattgaa aaaggacata aatccatggc gtatagcctg acattcgtca
    11461 acccagaagc aacattgaca gatgaagaaa tcaataaagc aatggaaaaa gtcgaaaaag
    11521 cgttggtaga aaacctagag gcaagtattc gctaagaagg aaaatatagt aagtaacgat
    11581 caagtagctt ttgaagacca tacatttgaa aaaaagatgt gacacaactt cgtcacatct
    11641 ctttttttga ttttaaaaaa ggaatagcac agagacgaga taaaaaaaca ggcctttaca
    11701 gcctgctttt ttagagtaaa tgaattaatg gaagtaattg attcccattg ctgattttac
    11761 ttcagaaagt gtttgtgcag ctactttagc agcttcttca ctacctttac gtaagatttc
    11821 cataacagct gaaggatctt tttccaattc ttctcggcgc gcacggatcg gtgcaaattc
    11881 agcttccaat acatcaatga ggtatcgttt gatctttaca tcaccaagtc cgccgcggcg
    11941 gtaatgagct ttcatttctt caattgcttc tttatctgtt ccgaatacat ccaagtaggt
    12001 gaagaccata ttcccttcta cttgtccagg atcttgtaca tggatgtggt ttggatcagt
    12061 atacatactc atgacttttt tctgtaagac atcggcagga tcggagatat aaataccgtt
    12121 tcctaacgat ttactcattt tgccatttcc atcgattcca ggtaatctac ccattccttt
    12181 tggtgggaat acgccttttg gttccaccaa gacttcacca tatgtatgat tgaagctttg
    12241 tacgatttcc tgtgtctgct ctagcattgg tttttgatcc tcaccaacag gtacaagatt
    12301 cgctttgaat gcagtgatat ctgctgcttg agaaacagga tagataaaga atcctgtagg
    12361 cacgctttca ccgaattttt tttgttcgat ttctgttttg acagtcggat tgcgccgaac
    12421 gcgtcctacg ctgactaagt tcaaataata catggttaat tcagctaatt ccggaatttg
    12481 tgattggata aataaagtag attttgcagg atctaatccc acggctaaat agtctaaagc
    12541 aacttgcaag acatttgatg aaaccttctc aggattttta gcattatctg tcagtgcttg
    12601 catgtcagca atcatgacaa acagttggtt gtcttcatct gcctgcatag ccactcgggt
    12661 ttttagagaa ccgacgtaat gccctaaatg cagcttccca gttggtcgat ctcccgttaa
    12721 aataatattt ttcaaaaaag acacctcttt acgatttgta agtctgagca cttaatatct
    12781 taccgttttt cctgaaaaga aacaacagtt cctaaggaaa aaacaaggaa agaggataaa
    12841 ataactaata gattagaaat ggaagaaata tttatgggag aataaacgtt ctaagaaact
    12901 ttacaaatta ctaaactatc agatataatg tattttatta tacatcttta aaagaaatct
    12961 tactaaataa taatgggagt gtaattctat ggcaatgatt aaatttgatc acgtggataa
    13021 atattatggc aagttccacg cattaaaaaa tatcaatctc gaattcgaga aaggagaagt
    13081 tgtcgttgtc attggtcctt ctggttcagg aaagagcacg atgcttcgct gtatcaatgg
    13141 attagagacg atctcttcag gtaagctgct gattaatgat actgacttgc atgataaaaa
    13201 gacaaaactg acagaagtcc gaaaaaacat cggcatggtt ttccaacatt tcaatttgta
    13261 cccaaataaa actgttctgg aaaacatcaa attagcacca atcaaagtgt tgaagcaaga
    13321 ccaggcaact gcagtcaaaa acgcggagaa atttttgaaa actgtcaata tgttagataa
    13381 gaaagattct tatccttcca tgttatccgg gggacaacag caacgggtgg caatcgccag
    13441 aggtttagcg atgaatcctg agatgctgct gtttgatgaa cctacttctg ctttggatcc
    13501 ggaaatgatt ggtgatgtat tagacgttat gaaaaaattg gctcatgacg gtatgtcaat
    13561 gattgtcgtg acacatgaaa tgggctttgc caaagaagtg gcggatcgtg ttatcttcat
    13621 ggctgatgga gaagtattgg aagacagcag aaatgtgaga gactttttcg aaaatccaaa
    13681 tgaagtccga gcacagcaat ttatcagtaa agttatcaat cattgatagg aggaaaaaga
    13741 tgcgcaggtt aaaaaaaata cgcctgattt tccttttagg cattgctttt cttgcactag
    13801 ccggatgtaa aggaaatggg ttggcaaatg aagatatttt agagcgaagc aaatctacca
    13861 atgaaatcat ctggggtgtc aagtatgata ctcgattatt tggaatgatg gatattgaga
    13921 gcagaactgt acaaggattt gatgtagata ttgcaaaagc aatcactaaa aagatccttg
    13981 gtgacaatgg aaaaacagaa tttgttgaag tcacttctaa aactcggatt ccattattga
    14041 aaaacggaaa tatcgatgca attattgcaa cgatgacgat cacagatgaa cgaaaaaaac
    14101 aggttgactt ttcagatgtc tatttcgatg ctggccaagc tttgttggta aagaaaggaa
    14161 gccaaatcaa aagtgtagat gatttgaatg cttctacaac ggttctagca gtcaaaggtt
    14221 ctacatcggc tgcgaatatc cgacagcatg caccagatgc aaaaatttta gaattagaaa
    14281 actatgcaga agcatttact gctttacaat ctggtcaggg agacgcgatg actacggata
    14341 atgcgatttt gttagggatt gcagatgaaa atccagagta tgagttagtt ggtggaacgt
    14401 ttactaatga gccttacggg attgcgatca acaaagggca agaaaacttc ttgaaagctg
    14461 ttaaccaagc gctagaggaa atgcatgcag atggcacata cgataaaatc tatcaaaaat
    14521 ggtttccaaa tgaaactgaa ggaaaagtag agtaaggggg agaggcaatg agcgaattga
    14581 tacaaactta tggaccaatc tttgtcgacg ggttcaagat cacattgttt tcgagtgtct
    14641 tagcacttgt gtttagtttg attatcggaa cgttaatggc aatcttccaa ttatcaaaga
    14701 ataaagtagt gagcgcctta gcaaaggcat atgtagaatt ttttagaaac attccattat
    14761 taatcatcgt gatgtttttt tatgttgtcg taccgattta ttggtttaat ctagatggat
    14821 tccaagcagg tacgattgga ttaacgatct atacatctgc ttttattgct gaaacagtac
    14881 gttcagggat tcaaaccgta ccaaaaggtc aaacagaagc aggactatct tctggtttta
    14941 cctattcaga aatcatgcgt tatattgttt taccgcaagc attcaaaatc gttatcccgc
    15001 cattaggcaa ccaatttatc aacctcgtaa aaaactcatc tattttagca atcgtagctg
    15061 gtttagattt gatgtatcaa ggagacttga tcgctagtga aacattcaat accttcgata
    15121 cgtatatcat cgttgggtta ttttacttag ttatcacgct accgttatct tacttaatgg
    15181 tttatcttga gaaaaaatgg gcggttcgtt catagaagga ggggaaatac atggatttta
    15241 gcggcgcttt ttcatggatg aatatccgtt ttttattgga aggattaaag gttactgttg
    15301 aagtttcatt gttttcgatc atttttagtt tcttgatcgg tggtctgaca ggtgtgattc
    15361 gttttgcgaa tatccctttc ttttccaaaa tccttggtgt agtgattgat attatacgga
    15421 atcttccatt attattgatc attttcttca cttactttgc cttacctcaa atcggcattc
    15481 agatgaacat cttctggtcc gctgtcgcag cattaacgat ttttgagtca gctatgttgt
    15541 cggaaatttt ccgagctgga ctcaatgctg ttccaaaagg acagatggaa gcaggattat
    15601 caacgggact gacttatgta gaatcgatgc gaacgatcgt gttaccgcaa gcatttaaat
    15661 caatgatccc ggcaattgtg agccaattga tttcactgat caaagatact tcattagcag
    15721 taatcatttc tttacctgaa ctttctcatc aggcacgtat tatttatgga cagaacacca
    15781 attatgtgct tccgatgttc atcatcatga cacttatgta cttcgtggta tgttacgcgc
    15841 tttctctgct ttccggttac ttggaaaaga gacggtacag ttattaatag atgattcttt
    15901 tagcggttgt ggcaaactaa agtcacaacc gcttttcctc tttccattcg aaaaaaagag
    15961 atttcgattg ttttaagcaa gtaagagcac ttgaaaattc acgctaaaaa gtacacaata
    16021 gggaggatag aatttattga aaaaaaggat gatacgattg acagataatc gccctatcgg
    16081 atttattgat tcaggtgtcg gcggcttgac tgtagtaaaa gaagccctga aacaattacc
    16141 gaatgaaaat attttatttg taggagacac agcacgctgc ccatatggcc ctagacccgc
    16201 ggaacaggta atccagtata cttgggaaat gacggattat ctggtggagc aaggaatcaa
    16261 gatgctggtg atcgcctgca ataccgcaac tgcggtggct ttagaagaaa tcaaagctgc
    16321 tctttctatt ccagtcatcg gtgtgatcct tcccggtact agagcggcag taaaaaaaac
    16381 acaaaataaa caagttggca ttatcggtac gattggtacg gtaaaaagtc aagcttatga
    16441 aaaagcactg aaagagaaag taccagaatt gactgtgaca agtcttgctt gtcccaaatt
    16501 tgtttcagtt gtcgaaagta atgaatacca ttcatcggtg gcgaaaaaaa ttgtggcaga
    16561 aacattagct cctttaacca ctaaaaaaat cgatacattg attttgggat gcacccatta
    16621 tccattatta cgccccatca ttcaaaatgt aatgggagaa aatgttcaac tgatcgattc
    16681 tggagcagaa acagtaggtg aagtatctat gctgttagat tatttcaatt tgagcaattc
    16741 accgcaaaat ggtcggacat tatgccagtt ttatacaact ggctctgcca aacttttcga
    16801 ggaaatagct gaagactggc ttggaatcgg acacttaaat gtagaacata tcgaattggg
    16861 aggaaaataa ggatgcgtca tgacgggaga aaagcccaag agcttcgcaa actggagctt
    16921 caaacaaatg ttttcaaata tccagaagga tcggtagtga tccgttttgg tgatacaaca
    16981 gttatttgtt ctgcaacaat agaaaattct gttccccctt ttttgagaga aacaggaaca
    17041 ggatgggtga cagcagaata tagtatgctg cctcgagcaa cgagtacgag aaatcgtaga
    17101 gaaagcagca aagggaaact ttctggtaga acaatggaaa tccagcgtct tattggtcgt
    17161 tccttgcgtg cagtagtaga cttggaaaaa ttaggagaac gtagtatcat cgtagactgc
    17221 gatgtgatcc aagcggatgg cggtactaga acagcaagta tcactggggc atttgtggca
    17281 ttgaaattag ccattgaaaa acttttaaga gaaaaagaat tgtcagaaga tccaataaaa
    17341 gaacatcttg ctgcagttag cgtaggaatc ttaccagatg ggacttgcgt cacagatttg
    17401 gattaccaag aagattccgc agcacttgta gacatgaatc ttgtaatgac agaatctgga
    17461 aaattcgtgg agattcaagg aactggagaa gaagcaacgt ttgatggtga acagttgaat
    17521 gaaatgctct tttttggaaa aaacgcgatt gaagatttga tcaaagagca aaaacatgct
    17581 ttgctaacag agtttgctca aaatgatgag cggatagaag aaacaaaaac aatcatcatt
    17641 gcgacaagaa atccaggaaa agcagaagag tttcgaaata tgttcaaaga agcgggatat
    17701 catgtaaaaa cattacttga ctatcctgaa ttgccagatg tggaagaaac tggcagcaca
    17761 tttgaagaaa atgcccgatt aaaagcagaa acgattgcac aattgcttga tcagccagtc
    17821 ttagcagatg attcaggttt gaaagtggat gctttaggcg gtatgccagg aatctattct
    17881 gcacgttttg caggggaaca aaaaagtgat gcggggaata acgccaaatt attatatgaa
    17941 ttgacagatg tgccggatga aaaaagaaca gcacaattcc attgtacgtt agtattcgcg
    18001 gcacccaaaa aagacagcct tgtagtagaa gcggaatggc ccggtcgagt agcccgtata
    18061 ccatctggcg aaaatggctt tgggtatgat cctttattta tcccagaagg taagaaacaa
    18121 acagcagcag aattatcgag cgaagaaaaa aacaagatca gtcatcgggc tcaagcaatg
    18181 aaaaaattga gtgccgagtg gaaacaatgg ctggaaggag agagatagat gcggtattta
    18241 gtagtaagtg ataatcatgg agacagagat atcctcgttg acttagtaga tcgttatcgt
    18301 tctgaagtgg atatcttttt ccattgtggt gattcggaat tagaagcttc tgacccatta
    18361 tgggatacat tccaagtggt tcaaggtaat tgtgattatg gaccaggatt cgaacagaaa
    18421 aaagtgatcc aaactggtca ggatacagta ttcatgactc acggtcattt atcgaatgtc
    18481 cgatttggtt tgacacagtt agcaatcgaa gctcgagctg caactgccga tatggtgttt
    18541 tttggtcata cccatcaaat aggctgtgag atggatcaga aaatcctgtt tttgaatcct
    18601 ggtagcattt ctcagcctag aggcccgatc caaatcccat catatgctct tatcgaaagt
    18661 ttagatgacc aaatttctgt tcaatactat aaccgtgccc atcaggcagt tgaagagttg
    18721 gccttttctt tttcgagaga gcgctgaata aaaaaactct aatttttaat aaaaataagc
    18781 tttattttat gaattttgca tccggtttcg gtaaaatagt agtgttgaat gaaatagaaa
    18841 tggaggccat cagatgattg gacctatagt aagagaaatg ctgctggaaa atcaggaaac
    18901 gtttttgatt cctgcagaaa atgtggcaaa tgttatgtat catcatccat tgtcccatgg
    18961 tttacttgtg ttatcgaagg tcggttattc caaaattcct gtattaggaa aagatgatca
    19021 ctttgtagga ctagtaagtt tatcagatgt ggtcaataaa atgattgact tgcaaacaat
    19081 cagcatggac ccgttagaag gactaacggt tgctgatgtg acagaaacag atgttccgac
    19141 aattgatgaa aattgggaac tagaagaagt actccatttg ttagtcgatt cgcctttttt
    19201 accggttgtc acagaagatc ggattttcaa aggaatcatt acaagaaaag agctgcttaa
    19261 agccgtaaac ttcatggtgc atgaattaga acgtcgtaat atattgtctc cgaaaatcga
    19321 agaattaaaa gaacaaatcg atatcgtgag ctaaagaacg agaaactaaa agataatcaa
    19381 aaaataagta gaaaaaacag cgtaccagtt ttgactttat cttgaaaaag aggctgggac
    19441 aaaagcgttt aactccaaga aataagaagg aattcacgaa aattgctttt caaatttttg
    19501 tgaatttcag cttatttccg aaggagttgc ttctgcttcc gccgtttata cgtgtttaga
    19561 gcgtgggaca aaagtgttct ttacttttgt cccacgctct ttttagtgag attcgttgca
    19621 tcgtttgtcc aaattatttt ccgacagcaa ttggactgtt cggaatcgtg aaaccagaag
    19681 ctgtcaattc tttgacatag gagctcaaga actcttcttt taaggcatat tgttttccgt
    19741 tcaatgcgta gcagaccgta cgaatcgcaa aattgctgtt tccaagatcc actaatccaa
    19801 agatagaagg ttcagtttgg agctcttctt ggtatttttc agctaatgta cgatttactc
    19861 gatcaatgat ctcatagatt ttgtcatatc cttcttcagg aacaatccga atatctaaaa
    19921 gtacttgcat atttgcacga gagagattgc tgatcgtggt gatgtttcga ttcgggatga
    19981 aatggacagt accatccgtt gctttcagct gaagggtacg gataccaaca gatacgacgg
    20041 ttccttcaat cgttaagttt gctagcttga tgtagtcccc aacatccatc tgttgttcca
    20101 tgataataaa aaatccggta ataacatcac tcatgaaccc ttgggcacca agaccgattg
    20161 ccacacctgc aattccggca ccagccaaaa gtgatccgat cggcacaccg atagcagata
    20221 aaattgcata gataaaaaag aaaccaaggg tataatgaaa aatagtattt aaaagactat
    20281 gtaaagtctt taagcggctc tcgtttagaa tggctttttt agaatactga ttaaaagact
    20341 tatcaatcag atattttccc acacggtgaa gaatccaaaa aaggacgatc aaaaaaatca
    20401 gatataaact tttttggata aaggcagcca aaatggcgtc ccaatcaatc ccgtcccaaa
    20461 accgctgaaa cgcatttact ttgctgacag cttgatctgt caatgtctcg ccagaagagt
    20521 ctgtcgtacc ggcagttgct aataatgaaa acataaaatc ctcttttcta aaatttgatt
    20581 ctttctgtat tgtaccaaga agttttcaaa aaaggaaata gcaaaaataa ctataatgaa
    20641 ggaaaaaagc tgaaaataaa atgacaaata atatggataa tgataaaatg tggagaaaat
    20701 aacgaaggtg gtaagtatat gcaaatcgat tggaacaatc taggtttttc ttatatcaaa
    20761 actccttggc gttttattgc caaatggaaa gacggacaat gggaagaagg agaattgaca
    20821 gaagataatt acctgacgat tcatgaaggc tctccagcgc ttcattatgg tcagcaatgt
    20881 tttgaaggtc taaaagctta tcgtcgaaaa gacggcaagg tcaatctctt tcgacctgaa
    20941 caaaatagtc gacggatgaa tcaaagtgct agacgattgc tgatgccaat ggtccctgaa
    21001 aagatgttcg ttgatgctgt caagcaagtg gtaaaagcaa atgaagattt tgtgccgccc
    21061 tatggaacag gcgctacatt atacttgcgc cctttactga ttggggtcgg tgaaaacatt
    21121 ggtgtccatc cagctccaga atatttattt gtcgtcttct gtacgccagt aggagcgtat
    21181 ttcaaaggtg gactaaaacc aacgaatttt attgtatctg attacgatcg tgctgctcct
    21241 aaggggactg gtgcagcaaa agtcggcgga aattacgcag cgagtttact tccaggaaca
    21301 gaagcaaaag aactggatta ctcagattgt gtttatcttg atccagccac acatacgaaa
    21361 atcgaagaag taggtgcagc aaatttcttt ggaattacaa aagacggaaa attcatcact
    21421 ccgcaatcgc cctctatctt accaagtatc acgaaatatt ccttgttgca gttagctgaa
    21481 gaacgactag gactgcaagc aattgaagga gatattttca ttgatgatct agatcaattt
    21541 gcagaagctg gtgcatgtgg tacagcggct gttatttctc caatcggtgg gatttatcac
    21601 aataacaatc ttcatgtatt ttatagtgaa acggaagtag gaccagtaac aaaacggttg
    21661 tatgatgaat tgacaggtat tcaatttgga gacatagaag caccagaagg ctggattgaa
    21721 gtcgtggaat aatttcaaag taatgagttg aaaaaaacgt aaaaagaaaa aatcaggaag
    21781 agactttaaa aagttttttc ctgattttta tatgttttac tttaaagaat gcaacaaaag
    21841 tttatgaaag atggacacct ttgtcaacgt agataatatc accgatgatt ccgctggaaa
    21901 gtgggctgat taagaaagca caagcattac cgacttcttc gatcgtcaca ccttgctgat
    21961 caggcgtacg agattcagat aactggatca gtttttgata gtctttaaca cctgtgacag
    22021 ctaatgtttt gattgcccca gctgagattc cattgacccg aattccttta ggcgagaact
    22081 cagctgccaa ataacggata gcagcttcaa gagaagcttt cgcaataccc atcatgttat
    22141 aattcgggat tgcgcgttct gaacctagat aagtcatgct aacgatcccg ctgtttggtg
    22201 caagaaacgg ttgtgcgtat tttgatacag caattaaaga gtagctgctg atatcttgag
    22261 ctaattgata gccgtctctg gaaatatcag acacatttcc ggacaattct tctttgttgg
    22321 cgtaagcaac tgcatggaca aggccgtgga tctccccagc atattcaccg atggcattca
    22381 ttgctttttc gatgctttcg tcgctagaaa catcacattc tacagtgaat gcttcttcgc
    22441 cgaccaattt gactaatgat tttttcattc gttcattttg gtaagtatag ataatttctg
    22501 ccccttgttt caacattgct tcagcacaac cccaagcaat gctttttttg ttagcgacgc
    22561 ccataatgac gatttttttt ccttctagaa atgacataat gactctcctt tgtttctgat
    22621 aaaaatttga tagtcgtaaa aaaatagaga ataatagcta aaataacttc ttgatttctt
    22681 aattattagt caaaatgttt tgattgtcaa actatttttt cttaaattaa aaaaagattg
    22741 ctaaatattt aggaatatga tactttgata gtcaaagtaa tgaaaatggc aattgcctga
    22801 aagaaggagt ttaattatgt cttacgtatt atctgctact gaagtaatgg aattgattcc
    22861 taatcgttat cctatttgtt atattgatta tgtagatact ttagaaccag gaaaaaagat
    22921 cgttgcgaca aaaaatgtaa caatcaatga agattttttc caaggtcact ttcctgacaa
    22981 tccagtgatg ccgggtgcgt tgattctaga aacactggca caagcaggtt cgatacttat
    23041 cctaaaatca gaagagtttc taggaaaaag agcttatatc ggtgggatca ataaggccaa
    23101 attccgtcaa aaagttgttc caggagatgt gctgaaatgt cattttgaaa tcaccaaaat
    23161 gaagggatca gtcggtacag cgttatctgc agcatatgta gatgataaaa aagtctgtga
    23221 gtgcgagttc acgttcatcg tcaaccaaaa tgcttgacta taaaacctga tatagcaaaa
    23281 caaaaggaag cttacttgct ttttatgtaa aaagaagacg cctgaatcct agcatacgtt
    23341 gtcatctatg caggattcag acgttatttt tttatttttg ttttgcttct tgcagtatat
    23401 tgattttatc gatttttact tcttcttttg gtttgtctga actatctgtt tcgatatttg
    23461 caatagcatc cacgacatcc attccttcaa tcacttggcc gaagacagta taatggccat
    23521 ctagtgaagg ataaccgccg tttttataag cgtcgatgat tgcttgagga tagtcgtctt
    23581 ttaaaagacc atcatgcata tcatctgtat tttgaacgat aaagaactgg ctgccgttac
    23641 tgttaggatc ttgtgagcga gccatagata acgcacctct tatattataa aggtggttgt
    23701 tgatctctgt ttcaaatcct tctccccaaa tgctttcccc tccagtgcca tcgcctttag
    23761 gatcgccacc ttggatcatg aaatctttga tgacgcgatg gaaagtcagt ccgttgtagt
    23821 aaccgtcttt tgcatgtgtc ataaagtttt caaccgcttt tggtgcgtgt tcgggaaata
    23881 atttgatttt gattgtgcct ttattcgtca ccatttcgac cagatcttca ttttcggcga
    23941 catctttcgt caattgtggt aattccaatg cgttcaaatc gacgctactg gcagtcgtgc
    24001 tttccgcagt agatgtactt tttgaacttt ctgtagacga actagaagaa gtcgtgttgt
    24061 ttgcagaaca accagcagca aacagcaatg ctgctgctaa actaatactt aaaataactt
    24121 tatttttctt cataactgtt cctttccaat ttactatttt gttatcttta atgataacaa
    24181 aatagaggga aaatacctac ttaaattatt ggaatttatc atttatttta aaagaaaaag
    24241 aaaaggtgta gaccatttac tttgtgtttg ctataatgta agtggataca aaaacgaaaa
    24301 gatgcaggag gaaatggtaa tatgggaaaa ttaggaatct cgatttatcc agaacggtca
    24361 acatttgaaa aggacaaagc gtacttggat ttagctcata agtatgggta taaacgggtt
    24421 tttaccagtt tgctgcagat caatgatgat aaagaaaaag ttctttcaga atttaaagaa
    24481 gtagttgatt acgcgaatag cctaggcatg gaagtaatgg ttgatatcaa tccagcgtta
    24541 tttgaacagt tggagatttc ttacgatgac ttatcttttt tccataagat gggtgcctac
    24601 ggggtccgct tagatatcgg attcacagga gcagaagaag caaaaatgac gcgcaatcct
    24661 tttggtatca aaatcgagat caatatgagt tcgggcacga attacgttga caacatcatg
    24721 agttattcgc caaacacgga taatttatta ggttctcata atttttatcc tcatcgatac
    24781 tctggtttag gatacgaaca ctttgttttt tgttcagaaa aattcagaaa atataatttg
    24841 aatacgatgg cattcgtcaa ttcgcaatct gctgagtttg gcccttggcc tacgcaagat
    24901 ggcttgtgca cgctggaaga ccaccgagat cttgaaattg cgacacaagt gaagcattta
    24961 atccttacag gattgattga tgatatttct attggaaatg cgtacgcaag tgaagaagaa
    25021 ttgaaagaaa tggcagaagc atttaacgcg gattatccaa cattgaaagt agatacggaa
    25081 gaaggtatta cagaaaacga acgaatctgc ttgtttgata accttcatag ttatcgaggg
    25141 gatcgctcag aatatatcct tcgttcaacg atgactcgtg tatactacaa agataaagat
    25201 ttcccgcctc ataatacgcg agatatgcat catggggatg tactgattga caatgaaggg
    25261 tacggtcaat acaaaggaga aacacaaatc gccttgaagg atatgaaaaa cgacggacgt
    25321 gtcaatgttg taggaagaat ctccgatgac gaattattcc tccttgactt tttgaaacca
    25381 tggtcaagct ttaaattaat tgaaaataaa aaatagataa ttagttattg cagaaataat
    25441 ccgctagaaa tagccagtta tttctgcttt tttattttgt ttttttatag gttatgattt
    25501 gtcaaattaa gctagaataa aatgcttctt gtacatagct caaaaatatt tcgattggtg
    25561 ttctgtaatt caatgatttt cttggaatat gattacgttg attgctgaca ctggaaataa
    25621 atgtctgatt cacttttcta aaatccattg atttcggcag tccattacga cgcagaatcc
    25681 cgttagaatt
//
LOCUS       DACDEG010000028        25534 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.028, whole genome shotgun sequence.
ACCESSION   DACDEG010000028 DACDEG010000000
VERSION     DACDEG010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.028"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            168..2228
                     /locus_tag="IV142_001260"
     CDS             168..2228
                     /locus_tag="IV142_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484929.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2311..2880
                     /locus_tag="IV142_001261"
     CDS             2311..2880
                     /locus_tag="IV142_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8484930.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3055..3861)
                     /locus_tag="IV142_001262"
     CDS             complement(3055..3861)
                     /locus_tag="IV142_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484931.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3999..5513
                     /locus_tag="IV142_001263"
     CDS             3999..5513
                     /locus_tag="IV142_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP8484932.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5916..6899)
                     /locus_tag="IV142_001264"
     CDS             complement(5916..6899)
                     /locus_tag="IV142_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP8484933.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6904..7551)
                     /locus_tag="IV142_001265"
     CDS             complement(6904..7551)
                     /locus_tag="IV142_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8484934.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7636..8268)
                     /locus_tag="IV142_001266"
     CDS             complement(7636..8268)
                     /locus_tag="IV142_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8484935.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8284..8835)
                     /gene="coaC"
                     /locus_tag="IV142_001267"
     CDS             complement(8284..8835)
                     /gene="coaC"
                     /locus_tag="IV142_001267"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP8484936.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8832..9569)
                     /locus_tag="IV142_001268"
     CDS             complement(8832..9569)
                     /locus_tag="IV142_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP8484937.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9566..10555)
                     /locus_tag="IV142_001269"
     CDS             complement(9566..10555)
                     /locus_tag="IV142_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP8484938.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10813..11355
                     /locus_tag="IV142_001270"
     CDS             10813..11355
                     /locus_tag="IV142_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8484939.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11372..12457
                     /locus_tag="IV142_001271"
     CDS             11372..12457
                     /locus_tag="IV142_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8484940.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12469..13275
                     /locus_tag="IV142_001272"
     CDS             12469..13275
                     /locus_tag="IV142_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8484941.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13276..14103
                     /locus_tag="IV142_001273"
     CDS             13276..14103
                     /locus_tag="IV142_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8484942.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14100..15173
                     /locus_tag="IV142_001274"
     CDS             14100..15173
                     /locus_tag="IV142_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8484943.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15601..18297
                     /locus_tag="IV142_001275"
     CDS             15601..18297
                     /locus_tag="IV142_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8484944.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18374..18862
                     /locus_tag="IV142_001276"
     CDS             18374..18862
                     /locus_tag="IV142_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8484945.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            18874..19368
                     /locus_tag="IV142_001277"
     CDS             18874..19368
                     /locus_tag="IV142_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8484946.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19389..20198
                     /locus_tag="IV142_001278"
     CDS             19389..20198
                     /locus_tag="IV142_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8484947.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20195..21016
                     /locus_tag="IV142_001279"
     CDS             20195..21016
                     /locus_tag="IV142_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8484948.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21067..23166
                     /locus_tag="IV142_001280"
     CDS             21067..23166
                     /locus_tag="IV142_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP8484949.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23163..24281
                     /locus_tag="IV142_001281"
     CDS             23163..24281
                     /locus_tag="IV142_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP8484950.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24320..25198
                     /locus_tag="IV142_001282"
     CDS             24320..25198
                     /locus_tag="IV142_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8484951.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25344..>25534
                     /locus_tag="IV142_001283"
     CDS             25344..>25534
                     /locus_tag="IV142_001283"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484952.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEF"
BASE COUNT         8069 a         4449 c         5405 g         7611 t
ORIGIN      
        1 aattaaaaag tacataagtt attattccga ttttcttgaa acaataaaaa atcaaaaaaa
       61 atactctcct aatagtccat cttcttttga ttttatttat ataatgaaat catcttatga
      121 aaattactac ttcatgttta gacaagagta gggaggacta agaataaatg tgttcaaaaa
      181 gacgttcaaa acaactaaat aaacacgctt gtcgtttaga taaggaacgg tcgtgttctg
      241 aaaaacacta cgttaagttt atgatgcgca cgatgctgat cagtttatct gtaggtgtct
      301 atatgcatgt acccgttcag gcaacgacac aaaccatcaa ggtcgtacgt acctctgttt
      361 ctgagaccac tgattcccct atcactatta ctcatacttt cactgatgca aatggaaaac
      421 agctacttcc tgcattgact tcctataata aagtcagtta tcctccaacg ataaaccact
      481 actggttaat tcctaacaaa acattatgga catcggaaac cggaagcagt gtgccgcttt
      541 ctatcttttc cgatatgact gatcctacaa aaggtgatca gttaaaacag atcactgata
      601 ttttgaatca tgctttgaca gatataaagg acggtggaag cgcacaagtt tcttatgtat
      661 atgaaaaaga ttattcacag tttgaaagaa agaaaatcga tgttctggca gggacaaaag
      721 tttcgatatc tgatctggta gaaaggctga agaacgcaga tggaacacaa ggagatatgt
      781 ccaaagtaat ttcaaacaat gggaatatcg tggatacgac taatacagga acatctacta
      841 tttccttgtc ctattttgat ccaattgctt taaaaacaat gactgcatct gctgtgatcc
      901 atgttcgtgc tgatcaaaca gctatcaaag gaaaagatct tcacttaaga acaggagaga
      961 gttacgcccc taaagatctt gttgaatatg taacaaaaag cgatggaact tcaggaaata
     1021 aagaagaaat caaaatgaac gggacagtag atactacaac tccgggagaa tatcccgttg
     1081 tattaactta tatagactcg aaaacatcaa atgtggcaga aacaacagct gtagtttatg
     1141 tagaagcatc tgctgcttca tcgactgtca tcactcattt tatagatagt caaacaaata
     1201 aagaacttca agcagaaaca catatgagta gtgtaaatcg tattcctgat ttgcgcctac
     1261 ctgctgaatt ttctgatcga gtgccaaata tagatttatc gacttatgaa caaaatggga
     1321 aagtcacttc tatttctgat tggatgcaag caaacggatt agacagaaaa agtcactggt
     1381 cggagatcct tgaagtactt cactcagaac tgatgaataa taggaagcaa tcaggagggg
     1441 aaattacctt tacttatgtt tatataccag acaatcgttc attagaaggt catgatctta
     1501 ttgttcatcc aaacgaaaaa ataaacaaag aagagttgat ccaatcagca acaaattcct
     1561 taggtaaacc cgtaccgtta aaggatgtat cggttacaat caatgaagta ccattggagg
     1621 atggttattt gaccaaacaa acaggtgatt ttacgattat gtatagttat catgacccgt
     1681 attcactcca agaactccat gctcagtcac tattacatgt tttaccatta aacaaacaga
     1741 tagatcaaaa agaacaaaaa agacaagaag aaagcttgtc agaaaaaaaa caacaaatgg
     1801 ctcatgcagt ttctccaaca gggaaagtaa aacaaacaag aagcctagag ttagcaagaa
     1861 tatctagaaa aatctcagaa tctaataaaa cgataaggca agaagctcat ttggccccac
     1921 aagtttctgg attggaaaat gaaattttag agcctaaaat aaaacaacaa tcagaaaaag
     1981 aatttgttgc taaacagtcg gaagatcaga aggaatcaga tgaccagaag gaaacgaaga
     2041 aaaaacgctt gtcaactatt gattccaccg atgagcatct gcagccgcaa gtaccacttc
     2101 ctagtccggg aacacccgca cctggtggag gtattccttc ttctgcttta ggagatttca
     2161 tgcgaggtat ggcaggaaca tgggtatacg ctaatcgaaa tgaatttgac gcagatgact
     2221 ggttctgatt tatatctcta tctgttatag tcaaataagt agctgagtca tatgttattt
     2281 ggcataatgg agacgaggag ggatagaaaa atgttagata aaacgttacc ttacgcagaa
     2341 atctggatga caagaccact tcaacaaaat gttccaatca cgcctttagc ggaaggtttt
     2401 gaatttcgat tttatcaaaa agaagatgaa tctgcttggg cggagattga gacttccgtt
     2461 ggggaatttc aaacgacaga tgaagcactt gagtattttc aacaaaactt tgctccttat
     2521 cctgaagaac ttgaaagaag gatgtttttt gtagagacaa aaaaaggaga aaaagtagca
     2581 acctgtaccg cttggcgaaa aaaatcaaga gagcaagtgt atccggtttt tcattggtta
     2641 gcagtgaaac cagattacca aggaaaaggg ctcgcaaaag cattgactgc cagagtcttg
     2701 caagaatttc ctaccctcca tacggacgga ccaatctatc ttcacacaca gacatggagc
     2761 catcaggcga ttgctcttta taaaaaaatg ggttttactt ttattgctga aaatctagac
     2821 ggcacaccta atccagatta tgaaaaagtt atgcaggttt taagtaaatt agataaataa
     2881 cgaaatggtg aaataagtcc tcagtattgg gataaaaaac gaaatagtca ttcctcatat
     2941 cttacttttt tgagttaata ctatcggata actaatggca tgccatacat tcaaaaaaga
     3001 aggggcagtg acaaaagtat tgtcactgcc ccttctcttg ttttttagca tgttttatcg
     3061 tttgattttt ttaggaataa aattcttgat gaacgtaaat ttttggtaat cgtttcgact
     3121 atagaggtaa ccaaatacac tgaaaacaac tgctccaata aaattaacaa ataaatcctt
     3181 catagtatcc atgattccca cgtctaagta accattttgg atgattgttt tatctgtttt
     3241 accatctgca ttttgacttt caatgattgt ccgagtgata tctggaacat gatagacagt
     3301 gtttccgtct ttacttaact ccacactatt gatttgctgg atgatcgtat ctttttgcat
     3361 atcgaattgc agtaatttat ctgctgaaaa ttccacaaac tcccacataa caccaactgt
     3421 catcgaaaaa cagaaggaaa caactgctag taataaagga gtcaagttca tggtttttgt
     3481 atttttattg agcaaataaa caagggaaaa gccgacaccc ccagctaaaa atccgctgaa
     3541 tgcatgtaga aaagtatccc acattgaaaa atagccataa aaatcactta actctcctaa
     3601 aactgttgaa ctaaaaataa aagataacaa aatgatttct agcatatttg gaatctgtat
     3661 ctttaatatc ttttccagaa aataagggat ttcaaataaa attaatgtca gtaataaaag
     3721 cattgtattt ccccagtgtt ggttccagac ctgatccact agcataataa cgacgatgat
     3781 acgtaataaa atatagattc cgtttgtgat tctacctgca gtttttgtac tgttcgtaaa
     3841 aaaggttttc aaatgattca taaaataact ccttcatgta tatccttaat tatactaaga
     3901 agcgtgtgtt taccaaagct atatgactta aagaaaaaca gagttgatat tgtgaaaaaa
     3961 atcacttaat atagtataag gagaaaaggg ggatacaaat gaaaaaatta gttgctggtt
     4021 tattaacagc cgttgcactt cttgctatga gcgcatgcgg cgcagatcag aaaaatacag
     4081 cgcagacaag ttctagtacg aaggaatctg ccgaagtatc atcaggcgct tctgaacaag
     4141 tatatacaga tccaaaagaa atgaaagaat cttatgatgt cgtgattatt ggagctggtg
     4201 gtgcagggat ggcagcagct atcgaagcaa aagatgctgg gttgaatcca gtaattttgg
     4261 aaaagatgcc tgttgccgga ggaaacacat ctaaatcatc aagcggtatg aatgcttcag
     4321 aaacaaaatt tcaaaaagaa gaaggaatca aagactcgaa tgatgccttc tatgaagaaa
     4381 cgctaaaagg tggtggagga acgaatgata aagaaatgct tcgatacttt gtcgatcact
     4441 ctgctgatgc ggtcgattgg ctagatcaaa acggtatcaa actggacaac ttaacgatca
     4501 ctggagggat gagtgaaaag aggacacatc gtccgtctga tggctcggct attggtggat
     4561 acttagtgga ggggttattg aataacgtcc atgagcggga aatccctatt tttgtcaacg
     4621 cagatgtgac agacattaag aaaaatgggg aaacggtcaa tgaagtgact gtacatgttc
     4681 aaggagaaga accaaaagag gtcaccggaa aagcaatcgt agttaccaca ggcggttttg
     4741 gtgccagcaa agaatttatt gaaaaatatc gtcccgattt agctgattat attacgacaa
     4801 atcaagaagg atcaactgga gatggtatca ccatgattga aaaattaggt ggacaaaccg
     4861 ttgatatgga taaaatacaa attcatccaa ctgtgcagca ggatgagggg gtcttgattg
     4921 gtgaggttgt tcgcggagaa ggagcaatct tggtcgatca agaggggaaa agatttgtca
     4981 atgaaatgga tacaagggat aaagtatctg cagcaattac agcattgcct gaaaaatcag
     5041 cttatttgat ttttgaccaa ggcgtgcgtg atcgtgccac agctattgaa ttttatgacc
     5101 aaaaaggata tgtcacaact ggaaagacaa tggaagaatt agcagaaaaa atcgatttac
     5161 caaaagaaac actagcaaaa acagttgatg gatggaatac ggatgttgcg gagaaaaaag
     5221 atgctcaatt caaccgtgcc acagccatgg aacatgactt atcgtctccg aattattatg
     5281 cgatcaaaat cgcaccagga atccattaca cgatgggcgg tgtgaaaatc aatacgaaca
     5341 ctgaagtatt agataaagaa aaccagccaa ttagggggtt atatgctgct ggtgaagtca
     5401 caggtggctt acatggaaac aaccggatcg gtggaaattc agttgcggag atcattattt
     5461 ttgggcgaca agcgggcata caagcagctc gatttgcttc ggaaaatgaa tgaaatagaa
     5521 cgtaaataaa aagaggttgt gaaatcagcg ttttgagcta ataccgaggg tcagctgatt
     5581 gaacactgtt tagtgaagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc
     5641 caagtaaaaa cagcttctta tgtttttaac ttggttggac ttattaccgc agatgcaagc
     5701 ttttgaacac cgtttattcg gaaaaagagc gtgggacaaa agtaaagaac acttttgtct
     5761 cacgctctaa acacgtataa acggcggaag cagaagaaat tccttcggaa ataagctgaa
     5821 attcacaaaa atttgaaaag caattttcgt gaatttcttc ttatttcttg gagttaaacg
     5881 cttctgtccc agcctctttt ttgatcagca tgattttatt cagttatctt caaaatgatt
     5941 tttccgatgt ttttgtttgc ttccatatat tcatgcgctt gtttgactgc gctgaattgg
     6001 aatgtacggt caatgattgg ccgaatcttg ccattttcta gaaaaggccc agctttttgc
     6061 ataaaagttt gtgcgagctc tgctttatac tgatcacttc taggtgtcaa taatgtacca
     6121 agcagttgga cacgtttccc aatcaataca ccaaggtcta tctcaggcac gatggtccca
     6181 cctagcatac caatcaagac ccaacgtcca tcaacggcta tagaagctaa attcttttcc
     6241 cagtaagaag cgccgatgaa atccaaaata acatcgactc cccgaccttc agtgattttt
     6301 cgtacctctt cttgaaagtc ttgtttttta tagttgatca attcatctgc accaagttct
     6361 ttacaaaagg cgagtttttc ttctgatcct gcagtaacga tgattttcgc gtgagaaagc
     6421 tgtctagcta attggatcgc tgctgttccc acaccacttg ctcctgcatg gatcaagaca
     6481 gtttcgcctt cttgtaattt tccaagccag taaagtgttt gataagcagt caaaaaaact
     6541 tctgagattc cagcagcaga aacatagtct agcgtatcag ggagaaggat tgcacgatcg
     6601 gcaggcatca cagcatattc ggcataacca cctagattga ctaatccgta tacttttgtc
     6661 ccaggagtaa aagtggagat atcactttta ttctcaacta cctctccagc tacttcgact
     6721 cccaaaatag ggtagggagg tttcaaagag actgattctc ttttcataat atctgttcga
     6781 ttgacagctg ctgtatggac tttgatcagt aattcgcctt gttttggttt aggaacaggc
     6841 acttcaattt gttctaatac atctacagaa ccaggatgct tgattgaaat ggcacgcatg
     6901 gatctattcc tcctttgccc attcttggaa tgctgtattc aactctttgc taccttcaga
     6961 aagtactaga gtcgtacgag aaatatccgt ttcttccact agagcggcaa gtacagcagc
     7021 aactgtagag cgagtgatcg tttttgatgc cttctcatta ggcttagtta attgaatgga
     7081 agtaacttct tcatcgttag tcaatgtgac tggttgaaga atgacaaaat ctaagttagt
     7141 tcgtttcagc cattcatctg cataatgttt agtaatataa taatctgtca tggactcagg
     7201 ccattttgct ggttcatctg cataaacagc actgaccatc agatagcgag agacatgcgc
     7261 attttcagct gcgatcactg ttttgattgc gccatccaag tctacttgca gaagattctt
     7321 tccttgagag ccggcagcaa aaatcaatac atcgatgcct ttgaatgctt cggtcatttt
     7381 ttccacggac caagttaaat caaaagagac atacgaaaca ttctcttttt ttactaatgt
     7441 ttgggaagca acatctctca cccctgcaaa aatttcatgg gaacttggag ctaaatcttt
     7501 tatcaggtgt cggccaattt gtccgtttgc acctacaaca aaaattttca tttggatcat
     7561 cctttcgacg tcaaatcttt ccattcaata gtaacaccaa aagagaaaaa aatcagagat
     7621 cggctacgat gactatcagt tagtaagaga agggtgcttg aagtatctta acaagacatt
     7681 gcatactgct gcagtcgcaa gacctgcgac gattccttca ggtataccac ttgtaaaaat
     7741 aaccgctaga atagctgaat aaacagcatt taaatttgtt ccgatgactt tggcataatc
     7801 ttgctggaat aaaaagtaga tcatattcat cactaaaatc gtattggtca atgaaccgga
     7861 aagtccagct aagaataaag aaacggaatc tgctttttgt ttcattagtt tgcgcagacc
     7921 tttataaacg aaataaggaa ccaccccaat caagatgcga ggaataaacg cgatcagcaa
     7981 ggctttccag cttccatgtt ctgttccgat gactgggata aacggagaaa agacaaaaga
     8041 caaaggtgtt tggataactg tgctacggat catgctgatc agtccaaatg taccgccaag
     8101 aaaagcacct agtcttggtc ctaaaacgat cgaagcaatg attacaggaa tatgcatggt
     8161 tgttgcattg attggaccga tagggataaa acctaaaggc gtaacagcta ataaaatcaa
     8221 aatagctaaa aacatcgcag ttaatgtaaa gttttttgta tttttcataa gtactccttt
     8281 tttctaagca tttctttcat taagagtcag catgacctgt tcgatgatcg tctgaatatc
     8341 agctaaggca ccttttccaa aatctccaca agctaaaaga gattctcttg gttcaatttc
     8401 ctgatagccg acagatctca atgtttccag attacgctga gtgatcgggt tttgatacat
     8461 attcgtgttc atggcagggg caatcaattt tggtgttccc actggaagcg ccattgcgac
     8521 tgttgaaaga agatcgtctg caatgccgtt tgctagcttg ccgataatgt ttgctgtagc
     8581 aggcgcgatg agaaacaagt cagcttgttt tgctagttcg atatgattga ttacctgcgg
     8641 atttgattct tgcatgacgt ctgtatggac tgcgcgcttc gacagagatt gcaaggttaa
     8701 tggcgtgata aaatgtgtgc tgcttttaga cataatcaca tctacctggg cacctaattt
     8761 gacgaattga cttgtaagat cagcgctttt gtaagcagaa atacttccag taacgcctag
     8821 taagatcttt ttcattttga ttcctccagt ctaattttct cagtaatcaa tgcagcaatc
     8881 tcagatttac tttgagcttc ttccactgtt ccatctttag ataagagata gccgtggtgt
     8941 gtctcgccat ggatttccgt caaatcattt gcgaaaataa aatcagtttt gttatttatc
     9001 agactatttt ttgcgactgc cagaagagat tcctttggta catctactaa tagtttgaaa
     9061 ccaatgattt ttgcttttgg ctgccattca cgtagataag aaatgatttt tggtgttttc
     9121 ttcaaaacga gcacaagatg ctcggtgtta gaagagatct ttgaaacagt ggtatctgtc
     9181 tgttcatttt ccgcaaacca attgtccagt tcttcttgcg ttgaagaagt gggtaggttt
     9241 tttgccagct gttcttctga aaaactaaaa gcaggagtaa aatcactgac tgccatactg
     9301 tggataatcg catcgtagtg ttcttttttc gttagttctt ctaattgtaa aaataagtcc
     9361 agtgcagatt cgatcgtata aatcgttata ttggagtgtc ttttcggttt cagtgcttgt
     9421 ttcgtagtca cgtaatcgat tgtggtatcc ggattggcag ctaaataatc agctaatgct
     9481 tgccctaatc gtcctgtcga atgatttgta atagcacgta cttgatcgat tttttcagtc
     9541 gttcctccag ctgtgattag aatcttcatt tatggtcacc tacatttact agtgttcttc
     9601 cttggtgtgt tccagcaagt agtttagatg ctgtttcagg caattgatcc agagtaatct
     9661 cttggacgac cagttcatct gcaatctgca aatccgccat ctgttgccat aaagaaagac
     9721 gttgatctat tggtacattg acagaatcaa ttccaatcaa ctggatgttt cgcagaataa
     9781 aaggcaagac agtcgcgttt agtttgattc ccccagcatt gccacataaa gctactgcac
     9841 cgttataaga gatcaatgga agaagactgc ttagctgctc gccacctact gtgtcgatga
     9901 catagtcgat ttgctgtttt cctaaagctt ttgttgtctc tggcagaaat tcatcaggtc
     9961 gtacgacttg caaagcacct agacttttta gccatgtgac agactcttct tttctggaaa
    10021 aggcaatgat tgactggaat cctagtttat gtaacagggc tatcgctgta ctgcctacac
    10081 cgccagaagc accagtaacg acaacttttt tatcggaggt cataccttga cggattaaag
    10141 cgttcacaca caacatagca gtaaaccctg ctgtaccaag gatcatcgct tgacgcagcg
    10201 tcatattttt tggtagagga actaaccagt ctcctggtac tttttgatat tggctataac
    10261 cgcctggatg ggtgacgcca agcccaaaac cagtagcaag tacaagatct tcttttgaaa
    10321 aacgtggatt gttactttgg acgacagttc cggataaatc aatccctgga gtcatcggat
    10381 atgttcgtat cacctgacca gactcgctca ttgccaatgc atctttataa ttgacatcag
    10441 aataggcaat ttttaccaag acttcatttt cagccaactc agtaaatggc agatcactga
    10501 ttgctgtatg gaaaacaggt tcctttatta cctgaaaaga tcgaaaagcg ttcattcgtg
    10561 agacactcct tttaaaattt tcttttatca taccataaag cctactcttt tattcttgtt
    10621 cttccgtaaa tttagacaaa aattaggtga aaaataaaga atcaaacaac tagaatttat
    10681 tccaaaagat aaagaaatat tcagcaaaaa aaattttaca aagggttgcc tttttcaata
    10741 aatttcgtat actaatacga gtggtagtaa acaggaagtt tactattaga aaacttcgat
    10801 tcggaggaaa ttatggaaat tggtgagaag ttaagaaatt tgcgcatcca aaagaatttg
    10861 acacaagaag aactaggcga acgcacagat ttgtcaaaag ggtatatttc ccagttggaa
    10921 cgagatttaa gttcaccatc aatggaaaca ttcttttcta ttcttgaagt cttaggtgtg
    10981 actccagaag aattttttca tcaagaagca gcgaatctgc aagtggttta ttcaaaagaa
    11041 gatcatacgg tttatttcga tgaagaaaac gggtatgaat tggaatggct cgtggctgat
    11101 tcgaatgaaa aagaaatgga gcctgtcttg ctgacttttg aaggtgcagg agaatataag
    11161 acgtttgaac cttcattatc tgaaacgttt atttttgtat tagaagggga attagagtta
    11221 agcttaggag agacggtcta tgtggcaaaa gcaggacagt cgatctatta tcaagcgaca
    11281 aaacaacatc aattaaggaa tcatctcaaa aaacgaacaa aagtattgat tgttgcaacg
    11341 caatcatatt tgtaatatag ggggtaaaaa cgtggggaaa ccaatcattt catttgatca
    11401 agtagtgaaa cgctatgatg atgaaacagt attaaaaaaa gtaagttttg agatcgaaca
    11461 aggaaaattt tatacactgc ttggtccttc cggctgtggt aaaacaacga tcctacgtat
    11521 tatagctgga ttcaccgaag ctacagaagg ggacatctat tttgaaggga aacggttgaa
    11581 tgatttgcca gctaataagc gacaagtaaa tacagtcttc caagattatg cactttttcc
    11641 tcatatgaat gtttttgata acgttgcttt tggattgaag atcaagaaaa tgtccaaatc
    11701 agatatcgaa aagaaggtaa aagatgcttt gcgtatggtc cagcttcctg gatacgaaaa
    11761 acgggagatt agtgaaatgt ctggtggtca aagacagcgt gtagcgattg cacgagcaat
    11821 tgtaaatgaa ccgaaagtat tgctattaga tgaaccgtta tcggcgcttg atttgaaact
    11881 acgtacagat atgcagtatg aactgcggga tctgcaacaa cgattaggaa tcacgtttat
    11941 ttttgttact catgaccaag aagaagcgtt ggcaatgagt gatgagattt ttgtcatgaa
    12001 taaagggaaa atcgtccaaa gtggtacacc cgtagatatt tatgatgagc caatcaatca
    12061 tttcgttgct gattttgttg gggagagcaa tatcgtggac ggtgtaatga tcgaagataa
    12121 tctagttgag tttgttggta agcaatttga atgtgtcgat ggcgggatgc gtctaaatga
    12181 accagttgaa gttgttttac gcccagagga tttgacgatc acgacaccag ataaaggaaa
    12241 actagtcgta acggtcgata ctcagctgtt tcgtggtgtc cattatgaaa tcatctgtta
    12301 tgatgaacag caaaatgaat ggatggtcca ttcaacgaaa aaagctaaag aaggaagcaa
    12361 agtaggattg gcctttgagc cagaagatat ccatgtcatg cgtttcaacg agtctgaaga
    12421 agaattcgat gcacgtcttg acagctacga agaatagggg ggacaagaat gaaaacaatg
    12481 cgcaggatct attccgtccc ttatattttg tggctcggtt tattcgtgat cgcgcctgta
    12541 ttaatgatca tttatcaatc tttttttgat atgaatgggc aattcacgct gaataattat
    12601 ctatcttatt ttacgtcggg gacatcgctt tctatgacga tcaattctgt atggtatgca
    12661 tttttgatca cgtttttcac gctgatcatc agttacccga ctgcttattt tttaacaaaa
    12721 ctgaagcaca agcaattatg gctgatgctg attattttgc ctacatgggt gaatctgctg
    12781 cttaaagctt acgcatttat tgggattttc agtgtgcacg gcagcgtgaa tcatttcttt
    12841 gattttatcg ggatcgggcc gaaacagata ttattcacag acttcagttt tctgttcgtg
    12901 gcaacgtata tcgagattcc atttatgatt atgccaattt tcaatgcatt ggaagagatg
    12961 aatccttctt tgatcagtgc tagtagagat ttaggcgcga atagctttga gaccttccga
    13021 cgagtgattt ttccgttatc attaaatggt gtgaaaagtg gtgtccaagc agtatttatt
    13081 ccttctcttt ctttattcat gctgactcgt ctgattggcg gaaatcgggt gatcacttta
    13141 ggaacggcta tcgaacagca tttcttggtt acacaaaact gggggatggg ttctacaatc
    13201 ggtgtgattt taatcattgc tatgttcatc gtgatgctcc tgacaggcga gaagaaaaaa
    13261 ggaggtcgct cataaatgaa aaaatttaaa tggtcgtatc tttatttagg gatcgtcttt
    13321 ttattcttat atgcaccgat cttctatctg attttttatt ccttcaatga aggaggaacg
    13381 atgaacagct ttacaggatt tacttgggaa aattatgaag ctgtttttga agatacacgt
    13441 ttgatcatta tcgtattgaa tacgttcatg ctggctttct tatctgcatt gatcgccacg
    13501 atcatcggga catttggggc tatgggaatc tattacacga aaaaacgacg tgcacggaca
    13561 gcgttgttga gcatgaataa catcttatta gtctctcctg atgttattat cggtgctagt
    13621 ttcttgattt tctttacact actaggattc ggattaggtt ttcagagtgt attgctttcc
    13681 catattgctt tcagcattcc aatcgtcgtc ttgatggtct tgccaaaatt gcaggaaatg
    13741 aacgattcaa tggtcgatgc ggctagagac cttggtgcta ataatttcca agtagtcaaa
    13801 aatatcattt tgccttatct ttctccagga atcatagctg gatattttat ggcatttacc
    13861 tattctcttg atgattttgc ggttacattt ttcgtcacag gaaatggctt tacgacgctc
    13921 tcagtagaga tctattctcg tgcaagacaa ggaatcagtt tagaaatcaa tgcgctaagt
    13981 gcgttagttt tcttgttctc catgatattg gtcattggat actactttat tagtaaagaa
    14041 aaccgtccaa aacggctgaa gaaaaacaga agagtacgcg agggggtggc acggctgcga
    14101 tgaaaaaatt acaatctctg tttttaggta ttctcatcat tattgcggtg ctgctgcttg
    14161 gtgttcgtca acttgaaaaa acaagtggca tgagtggagc aaaggtactt accatctata
    14221 actggggaga ttatatcgat cctagtttga tcacgaaatt tgaaaaagaa tatggataca
    14281 aagtgaatta tgaaacattt gattctaatg aagcgatgtt cacaaaaatc cagcaaggtg
    14341 ggacaaacta cgacatcgct ataccaagtg aatatatgat ccaaaaaatg atcaaagaaa
    14401 aactagtact tccattagat cattcgaaga tcaaaggatt aaataatatt gatccacgat
    14461 ttttagactt ggattttgat ccagataatc agtattcgat tccatatttt tggggaactt
    14521 tagggatcgt atataacgat aaattctttg atgcaaatca aattaagcat tgggatgatt
    14581 tgtggaagcc ggaattaaaa gacagtttga tgctgattga cggtgcacga gaagttatgg
    14641 ggctttcttt gaatagttta ggctattctt tgaatagtaa gaatatggca cagctgacag
    14701 aagcagcaaa taaattgaat catttaacac caaatgtcaa agcaatcgtg gcagatgaga
    14761 tcaaaatgta tatgatcaat gaagaagcct ccgtggcagt gacattttct ggcgaagctg
    14821 ccgatatgat gtgggaaaat gaacatttac attatgtgat cccaccagaa ggatccaatc
    14881 tttggtttga taatatcgtt atgccaaaaa ccgtgaaaaa taaagaaggt gcctatgatt
    14941 tcatcaactt tatgctagaa ccggaaaatg cggcacaaaa tgcagagtat attggttatt
    15001 ctacaccaaa tgaagaagca aaagcgttat tgccaaaaga catttcgtcg gacgaacaat
    15061 tctatccatc tgatgacacg atctcccatc ttgaagtcta caaagatcta ggatcgaaat
    15121 acctaggcat ctataatgac ctattcttag aattcaagat gtataggaga taatggcaaa
    15181 actcagtaag aatggggact tatcaacgtt ctaaagagct aaaaatcgtc ctttttctat
    15241 ctgagttttc caatcttcta ccaaaagcgg atgaaacgcc gctttttttg tgttatttta
    15301 ttttttctac cacgtttttc taccagagaa aagtggacaa aaccattgta aaatagggcc
    15361 cgagacgggc tagtaaatct aagattgaaa aagttacaga aggataaagt aattggaaac
    15421 tatgcaaata tacttaaccc ttctgtaatc tattaataaa actaatatat gaatccagtt
    15481 caatatttgt agctatgaaa aaacataaaa atcatattag ataaaaacta aattggtaga
    15541 taaattattt tgtcagatat ctgaggcaaa tattcgacta tttgtgtttc aaagtaaaaa
    15601 ttggaataca aatatagaat agacatattg cagccgatag tttattatgc ttttagacac
    15661 attaaactgg aggaaaaaat gaaaaagagc gctaaagatc gagtttttga tgagctgata
    15721 cgactaacag ggaatatgtc ttctgtcact gccgagaaaa tttcgaagaa tctaggtatc
    15781 tctcgtcaga atgccagcca ttatttgacg agattagtgg aagataaaaa ggtcgagaaa
    15841 ttaccgggaa agcctgtttt gtggaagcct ttagatgaat atgcggttgt agataatacg
    15901 gagcaaataa acgaagcctt tcattctgta gttggtcatg acggaagtct gagagaggtc
    15961 attcaaaaat gtatagccgc tgtcaaatat ccgcctaacg gactcagtgt attaataaat
    16021 ggagccacgg gtgttggaaa aagtttttta gccacaaaaa tttttgaata cgctatccat
    16081 gagcaaatta ttgaaaaaga tgcaccattt gctatcttaa actgtgccga ttatgcggac
    16141 aatcccgaac tgctttcggc aacactgttt ggatacaaga agggggcctt tacgggggct
    16201 gaaaaggata ccgatggttt attggcaacg gccaacaacg gctatctttt tctcgatgag
    16261 gtccatcgcc tatcgaaaga aaatcaagaa aagctctttc tatttattga tactggaaac
    16321 tatcgtccag tcggtgaaaa tgtaaattgg cattctgcta aggttcgttt tattttcgcc
    16381 acgacggaaa aaggtgaaaa ttatttatta gacacctttg atcggcgtat tcaaattagt
    16441 gttatgttgc caacttttga agatcgcccc attcgagaac gattagagct gattcaatta
    16501 ttttttcaaa atgaagcgga tgttttacaa aaagatatta ttgtttcaag agaagccttg
    16561 tctttaatgt tatctcatcc ctttagtggg aatattggta agctgaagaa tatcattaaa
    16621 atcagttgtg cggatgtgta tagtcgaaca caagaagaac cattaacgat tgggaaaaat
    16681 gagatcttga ttcagttaaa tatgctggaa tccgaagtag aaagcttgcc gattgctagt
    16741 ttattgataa agaaaaatca aaagataaaa acggatcgaa tcaaaccgtt tgattatgat
    16801 tcgattttgg aaaaaattac tcagctgata aatgaaggta atttcaatga aaatttttct
    16861 tcgataaaga atgaagtaca gcatttggtg catcacattg ataaggattc ttttttatca
    16921 atgagtagaa aattgatgat tcagatgttt agaaaggtct gggtatccgt tttaggaaaa
    16981 acgtacggat tgacgacgag tggacctgtt gcagaaatta ttagcagatc ctatgtcatt
    17041 tccccaagct ataagacaga cttaaccggg ataaatcgta cattagctgc aaatctacct
    17101 cggacgactt acttatgtaa tcagttcttg gagcatttac caccgctctc agaaaaggac
    17161 gagaactttc taagactgct tattgtcgtg actttatctg attatgtaga tgaatccatc
    17221 gagctgaagg gcctgttgct tgctcacgga gatagtacgg ctagcagcat tcaagcagta
    17281 gtgaatcaat tatgcggcaa ttatgttttt gaagcgttgg atatgccagt agagagtaat
    17341 gtgtcagaga ttgtcgaatc gacaaggaat ttcgtgaaac ggcaagtgaa tacagatagt
    17401 ctagtgctga tcgtagatat gggctcttta aatcaaattt acacacagat caagaacgat
    17461 ttaaaagggg agttacttct tctgaataat ttgacgactt ccattgcact tgatattggc
    17521 ttgaagatga cgatgaatgc gccatttaaa cagattgcgg aggatgcagc agacaagtat
    17581 tcaatcaatg ttcaatactt tgaaggcttt tctcaaagta gcaacattat catttcctgt
    17641 atgtcgggtt tggggatttc ggacaaattg aaagatgttt ttcgtcatca tttatcggat
    17701 gaatccatcg aagttttcac gaaggactac agagaactac gtgaattaat cgaccaaaat
    17761 gatgaaactt attttgacaa gacaaaattg gtcatcacga cttcggatct tccacgttcc
    17821 ttttcaattc ctaatattaa tgtttatgac atcttagacg gtgatggagc agccaatttg
    17881 gagcatattt tgagtcctga aatgaagccg gcagaattta accgcttgat tcaggagcta
    17941 gtaaaattct tttcgttgga aggaattgcg gaccgtttga actttctgaa cccaacgatc
    18001 gtgatgagtg aagtggaaac agtcatttct aaatatgaaa attactatca tttcacttta
    18061 aatggccgtg taaaactaaa tctctatatg catacggcat tgatgatgga acgtttattc
    18121 ttggctcgga cagaaaaatc tgaagaaccg aattatgatt tgaaaccaga tgaagatgaa
    18181 ttttattatg tttctagaag tatttttcaa tcaatggaga tgaaatataa ttttaaagtc
    18241 aatgcttatg agctttcgct gctctatgaa ttattaggac cgtatattca aaaataaagt
    18301 gtctaaaaaa gtgtctaatc tgtttttttt aggttagaca ctttttttaa tgtgaattaa
    18361 cgtgattaaa ccaatgtttt taaaagttgg cacaggaatt gcttatataa aagtgaacag
    18421 aaagaaagga attaaccata tgaagaaaat tctaatagcg actcacggtc acttagcttc
    18481 aggctttctt agcagtattc aattactgac ggggaaggtt caagagatta cggtgattaa
    18541 tgcttatgtg gatgagtgtg attttgaaca ggagttgaat gtatttgtaa accaggtgaa
    18601 cgagcaggat cagatttttg tttttaccga tttatttggc ggaagtgtca atcagaagat
    18661 taccaaaacc tttttggaga aagagattgc tgtaactatt ttgtctggct ttaatctgcc
    18721 aattctgctc gaaatagtat tggcaaccaa ggaattaaca aaggaggata ttcaggaact
    18781 ggtaaataaa tgccaacagg aattaaagat gaccattctt gatgaggtac atgtagtaaa
    18841 tgaagaggaa gatttttttt aggaggaaga aaaatgatta aagcagtgcg aattgatgaa
    18901 cgattaattc atggacaagt agcgatgact tggacaaaga gtctaaattt aaccgggtta
    18961 gtagtagcaa gcgatgaggc ggctaccaac gatattcaaa agatgacatt gaagatggct
    19021 gcaccatcga atgtcaaagt cattattaaa acagttgatg acgcgattca tttgttaaaa
    19081 gatccgcggg cgaaggacat gcgcttgatg gttttagtcc ctaatgtcaa agatgcggta
    19141 aaagttgcga aagctttccc aacggaaatc gaattaatga acgtggggaa tgctgggaaa
    19201 atgacaccgc catcggaaga taagaaaacc ttatcaaagg aagtgatgtt aacgacgtca
    19261 gaaattcaag acttaaaaga attagttgaa ctttatccag ataccgtttt tcagcctaca
    19321 ccggcgatgg aaaaacgttc agctatctct attttgagtg atttttagaa aaaggaagga
    19381 gaaaaattat gtatgatgca tttgtagtcg ctttggccgt ttttattgga gttgctggac
    19441 atgaaatgtt cgggatgtcc atgctgagtc gtccgattgt tgttgctccg ttagcaggag
    19501 cgttgatggg ggatttgcag acggggttga ttgtcggggc gtcactagaa gccatcttta
    19561 tgggtgtcgt caatatcgga atctctagta cggcggagcc ttctttggct gctgggctgg
    19621 ctacagcgtt cgcaatcaaa atgggaggaa acgtggatgc gattattccc attgtctttc
    19681 cactagcagt attaggatta cagttaatga atatgatttt ttcttttgta tgtggaccga
    19741 tggctgctaa atttttcaaa tttgcaaaat ctggtgatca aaaaggaatc atcaatctgc
    19801 acttctttat gtggtttgtc cactatgcgc tttatgctct tattccattc tttgccgttt
    19861 tatttggagc agatgttgtc aaaagcgtct tggataatat tccagaggtc atcatgaacg
    19921 gattaacagt cgctggaaat ctgctgccgg cagttgggat ggcgatgctg ttacggatgc
    19981 tttggagtaa aaatattgct gtgtggtttt tctttggggc cgttgttatg gcgtatctcg
    20041 agttaccatt gattgcaatt gccgcagttg gaacgattat tgcaattgtt atagcccaaa
    20101 gagatctaca actaaaaaat atatctgttc atgcagttca aagcgatgct gctgtaaatg
    20161 caacgcttga ccaagaagag gaggatttct ttcaatgaaa ttagctgatg gaatttctaa
    20221 agatgagaaa aaattggttc ggaagatgtt ttggcgctcg atgactatgt atgcttcggt
    20281 gaatccggtg acgatgggcg gcggtggttt cgcctattct atgatgccct tcattaatca
    20341 tttctacaaa agtgaagaag atcgagctgc ggcgctggaa cgtcatacac agtatttttc
    20401 gacgaccatt ccttgcgcgt cctttgtcat ggggattgcg gcatcgatgg agaaggaaaa
    20461 ttctgaaaaa gctggttttg acgtggaaag tatcaacgcg atcaaaacga gtttgatggg
    20521 accattggct ggaattggcg attcattgtt ctggggagtt tggcgtgtcg ttaccgcggg
    20581 actagcgatc aacttagcca atgccggaaa tattttggca ccaattatct ttttagttat
    20641 gttcaatata cctaattact tgatgcgata ctatggcggc gttttaggct attcattagg
    20701 ctctaaatat attgaaaagc tgtatgcgaa cgggttaatt gaaattctga caaaagctgc
    20761 tagtatcttg ggtctgatca tggtaggtgc aatgacgatc agtatggtca aattcaatac
    20821 cgttttgagc ttttcaatga agggcgaaga actaatgaat gtccaagaaa tcctcgatag
    20881 tatttttaaa ggaattgtac cgttattggt aacattgagc tgtttcaaat tactagataa
    20941 aaaagtcggc gtcatcaaaa ttatttttgg aatggtcgtc ttgggaattt tattgtcatt
    21001 acttggaatc gcatagtagg aagctgcagc tactaggctg cagttttttt agcaaggagg
    21061 gaaaagatga ctattaatga agtaatttat tcacccaaac acccattttt taatgctgag
    21121 ttaaacgacg gaaatgtgac tacttcaaac gaatacagct actatcggaa tattcctgat
    21181 gtaaagggat tgaccttcga tgtaagtaat caaaagacat tagcaaacct tgatgtttgc
    21241 gggacgattc aaacgttgac cttttatcga gaaaatttac taactgagga aaaaccaggt
    21301 gtttgggtca ataaacagct ctctcaaacc aattcgttat ccttaaaggt tgaagtaaac
    21361 gggattgtct accatttgtc agaatcggat catcgtattg aagtcgatgt gattcaagac
    21421 tgtttgccta gatatattca tcattattcg acgtttaagg tcattgtagt atcctttgct
    21481 cccatactgg ctaataagcg tttaagtatg cttgttcaac aggtttacat tgtgaatgaa
    21541 acacacgaaa gccttcaagt ttcagtgaaa gatgtgcctt cgtatcaaga gaagtattcg
    21601 gatcagcaaa atgtgttgat ttctcaaaaa ggaaataagg acacgatcgc acaacaggaa
    21661 gtggcaagct tcgccattgc ttttgtggac ccaaatgctt ttgaagaagt aatgactttt
    21721 caggaatctg atatcgaaaa atggctgaag gaaacattga actattttgc tcagctattt
    21781 gggcagctgt caatgccaaa cgatagagtt gtccatctct atcgtcgtgc actttatcaa
    21841 agcttttcta gtttcggtat gaatcgacag ggacaggtcg tcggcagcaa ttggggcagc
    21901 tatcctgcta ctaatcgaat ttggaataag gatatgtatt acgcatcatt gccattcacc
    21961 atgtttgaac cggagttatg tcaaaaaacg gttctatggt ttgatcaata tggcgttaag
    22021 tttcctggtt caaaatttac aggaggtatc aatcattctc tgagtaattc gttatccagt
    22081 gcgttactgg ctagtcttta ttttgaacat actggggata caaaattctt tgagcggtgt
    22141 ccgcaggttc tactgaatgc ttcaagaatt attgaggaca tattagctca acgtcggtca
    22201 ggggaaccaa tgctttttcc atctgtttgg ttatcagacg cctttgcact tggaaagtat
    22261 cataccggca gcaatctttg tctttggaag gcctgtattg gacttgcaga aatttttgaa
    22321 gccctagaac aactgagtct tagcaagcga tacaaaaaca ttgcctgtaa actaaaggaa
    22381 tcaatttgta aacaaatgac tatcgatggt ccttttggtg agcaattttt ggagggaatc
    22441 ggtgatgagg agaaaacaac ctattcagtg aagcactatc aaaaaccgat tcttgagcaa
    22501 gggttaatct ttttaagtga tgtgattgtg gatggaaaaa ttaatttatt gatgcatgat
    22561 ggagaagaat cagacactac gttgatgcct ttttatcaat ttttagataa taaagatcat
    22621 ttatatcaga acaccatggc cttttctgct agttctttca atccaaccta tagcgaggaa
    22681 atcaaaggca tcacttgggg attagaatcc ggcgccactt ttcctggctt tatcactgtt
    22741 ttaatgagcg agctgcacaa ccctcaagct ttcgtaaccc gattagaaga actaatttgt
    22801 ttggcagatt tagacggttc gtggtggtgg tggccgtatc gcttagaggc tcaacaagga
    22861 gaagtcgtgc gagactttgg ctgcggcaaa tgcggctggg catcgggtct ttttgtcagc
    22921 ctaatgatca gtcaatattt cggattgaaa ttcaaaaaag gagtattgca aattgatcct
    22981 gttgataatt taacgttttc ttggaagaat ttgaagttcg gtcaagcctt catttcgatt
    23041 gaatgtacgc aatccgagtt gagtatttca aatttgtcag aaaagccatt gaaaataagt
    23101 gatacgaaaa aaattcttca cgtagaaaag ggaaaaacga ttcacattca aaggagaaaa
    23161 agatgagtat actaaaaaat attacccaat ttaaaaaaat ccagagtcga gcaatcactc
    23221 ccgaaaatcc aacaggcgag aaaggtaaag ctgcgatgtt gccaagcgag ctaggaccaa
    23281 gccgcaaagg tcgtggcgca attccgctga cttctggtga agaaactgtg attgcggata
    23341 ttactggaac gggaataatt cgtcatatgt ggatgacgat tcgtgatcga acagaatttg
    23401 gcagttttgt tttacgtgat ttaatcttaa gaatttattg ggatgggtcg gatagccctg
    23461 ctgttgaaag tcctttaggg gatttctttt gtaatgggtt tggagagcgt tgcgaggtga
    23521 actcgttgcc gatagtggtt aatcctacag ggggctttaa ttcctatttc gaaatgcctt
    23581 tccaaacatc cgctaaaatt acgatcgaga atttacatcc gaaggatatg tcctcatttt
    23641 tttatacgat aaattacgct ttggtggacg aattggaaga aaatagccta tattttcacg
    23701 caaattggca tcgggaacgc attaccaaat tacaggagga ttataagtta attgatcgat
    23761 taaagggtca aggctattac gtgggtactt accttgcgct tacctgccta gaacgttatt
    23821 ggtggggcga aggcgagttt aaattctaca ttgatgggga tagtgagtat ccgaccgtaa
    23881 cctcaacagg ttcagaagat tattttggcg gggcgtgggc ctttcatgag tacgatgagt
    23941 tcggacgtcc acatgcgaaa aactattcaa cagccttttt agggtatcct taccaatcaa
    24001 atagagatgc cacaagagat cgttttgaaa cgggaaaatt gaatgccgtt catgcctttg
    24061 gtgacgacgc aatgccacga catggtcttt atcgttggca tcttcaagac cccatctgtt
    24121 ttaccgaaga tttatcggtc actttacaac aaatcggcaa cgatgatgtt cgcttatttg
    24181 agcggcaaga cgatgttgct agtgtcgctt actggtattc aacagaagct tcaggaaata
    24241 ctaaacgtca tttcactgat cgagaacgct tgccgcgtta atgaagtgtt tttcacatat
    24301 tcgaagggag gaacctatga tgatatatgg ggcaattgaa gctggaggga ctaaatttgt
    24361 ctgtgcagtc ggtactgagg aattaacgat tttggaaaga gtcagttttc ccacaacaac
    24421 tccagttgaa acaatgaagc aggtcattga tttttttaaa ccttttaagg aacaattggc
    24481 aggcatcggg gtcggctgct ttggtccaat tgatattcaa tgcaaatcaa cgagtttcgg
    24541 tcatatcaca tcaacaccaa aacttgcttg gcaaaatttc aattttgtag gaacgctaaa
    24601 gcaatatttc aatattccga ttgaatggac aacagatgtc aatgcggctt gctatggtga
    24661 atatgttgct ggttctggca aagggttagc aggtgttgca tacttcactg tgggaacagg
    24721 tatcggaggt ggtgccttgt tcaatgggac tttcgtggaa gggttcagtc atccggaaat
    24781 ggggcatatg ttgatcaagc gacatccaaa ggacaatttt tcaggaaact gtcccttcca
    24841 ccaagattgt ttagagggga tggcagcagg accagcgatt gaaaaacggt tgggtgtaaa
    24901 aggccagaat ttattggctg acgattcgtt ttggcagatt gaagcctttt accttgccca
    24961 atgtgcatac aatacaacac tgatgttttc gccagataga attatttttg gcggtggtgt
    25021 aatgaaacaa gaacacatga agaaaaaagt tcaggataaa tttgttgagc ttataaatgg
    25081 atatgttgaa ataccaccaa ttgatagcta cattattacg cccgaattag gtgataatgc
    25141 cggaataatc ggcggattag cattggccag aaaagcggta agaaataagc aaccataatt
    25201 aatgaatgtt tcattgatac tcatgtcaaa agtatttaac cgagtaatca aatgatcaaa
    25261 tgaagttgga taaagcgaaa atgaattcaa ataaaaaggc tctaagtcaa atcgtgttgt
    25321 tttcattaaa tcactgaaag gaaatgcttc tgcgtttcct tttttctttt tgttgtgcgc
    25381 tgtatacctt gaggatattc actggtggcg tacacttaaa tagtaatata ttgtgttact
    25441 ttatgtctgt ggtaatagtt atagttgctt accaatgtat gttcattttt cgaaatgttg
    25501 acttcataat ccttggctta atgtttgaat tttt
//
LOCUS       DACDEG010000029        24695 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.029, whole genome shotgun sequence.
ACCESSION   DACDEG010000029 DACDEG010000000
VERSION     DACDEG010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.029"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(111..959)
                     /locus_tag="IV142_001284"
     CDS             complement(111..959)
                     /locus_tag="IV142_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8484953.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSLKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1183..3123)
                     /gene="thrS"
                     /locus_tag="IV142_001285"
     CDS             complement(1183..3123)
                     /gene="thrS"
                     /locus_tag="IV142_001285"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP8484954.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3460..3639)
                     /locus_tag="IV142_001286"
     CDS             complement(3460..3639)
                     /locus_tag="IV142_001286"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP8484955.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3686..5509)
                     /gene="cadA"
                     /locus_tag="IV142_001287"
     CDS             complement(3686..5509)
                     /gene="cadA"
                     /locus_tag="IV142_001287"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP8484956.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5787..8066
                     /locus_tag="IV142_001288"
     CDS             5787..8066
                     /locus_tag="IV142_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8484957.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8159..8806)
                     /locus_tag="IV142_001289"
     CDS             complement(8159..8806)
                     /locus_tag="IV142_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8484958.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8985..9704)
                     /locus_tag="IV142_001290"
     CDS             complement(8985..9704)
                     /locus_tag="IV142_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8484959.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9835..10986)
                     /locus_tag="IV142_001291"
     CDS             complement(9835..10986)
                     /locus_tag="IV142_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP8484960.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11203..11943)
                     /locus_tag="IV142_001292"
     CDS             complement(11203..11943)
                     /locus_tag="IV142_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8484961.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11947..12270)
                     /gene="rsfS"
                     /locus_tag="IV142_001293"
     CDS             complement(11947..12270)
                     /gene="rsfS"
                     /locus_tag="IV142_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP8484962.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12327..12920)
                     /locus_tag="IV142_001294"
     CDS             complement(12327..12920)
                     /locus_tag="IV142_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8484963.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12904..13551)
                     /locus_tag="IV142_001295"
     CDS             complement(12904..13551)
                     /locus_tag="IV142_001295"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP8484964.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13564..13875)
                     /gene="yhbY"
                     /locus_tag="IV142_001296"
     CDS             complement(13564..13875)
                     /gene="yhbY"
                     /locus_tag="IV142_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP8484965.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13891..15000)
                     /gene="yqeH"
                     /locus_tag="IV142_001297"
     CDS             complement(13891..15000)
                     /gene="yqeH"
                     /locus_tag="IV142_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP8484966.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14997..15524)
                     /locus_tag="IV142_001298"
     CDS             complement(14997..15524)
                     /locus_tag="IV142_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP8484967.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15704..16489)
                     /locus_tag="IV142_001299"
     CDS             complement(15704..16489)
                     /locus_tag="IV142_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP8484968.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16482..17351)
                     /locus_tag="IV142_001300"
     CDS             complement(16482..17351)
                     /locus_tag="IV142_001300"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP8484969.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17352..18731)
                     /locus_tag="IV142_001301"
     CDS             complement(17352..18731)
                     /locus_tag="IV142_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP8484970.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18739..19158)
                     /gene="fabZ"
                     /locus_tag="IV142_001302"
     CDS             complement(18739..19158)
                     /gene="fabZ"
                     /locus_tag="IV142_001302"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP8484971.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19183..19659)
                     /gene="accB"
                     /locus_tag="IV142_001303"
     CDS             complement(19183..19659)
                     /gene="accB"
                     /locus_tag="IV142_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP8484972.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19662..20897)
                     /gene="fabF"
                     /locus_tag="IV142_001304"
     CDS             complement(19662..20897)
                     /gene="fabF"
                     /locus_tag="IV142_001304"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP8484973.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20916..21653)
                     /gene="fabG"
                     /locus_tag="IV142_001305"
     CDS             complement(20916..21653)
                     /gene="fabG"
                     /locus_tag="IV142_001305"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP8484974.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21665..22582)
                     /gene="fabD"
                     /locus_tag="IV142_001306"
     CDS             complement(21665..22582)
                     /gene="fabD"
                     /locus_tag="IV142_001306"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP8484975.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22689..22913)
                     /locus_tag="IV142_001307"
     CDS             complement(22689..22913)
                     /locus_tag="IV142_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP8484976.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22966..23928)
                     /locus_tag="IV142_001308"
     CDS             complement(22966..23928)
                     /locus_tag="IV142_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP8484977.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23938..24378)
                     /locus_tag="IV142_001309"
     CDS             complement(23938..24378)
                     /locus_tag="IV142_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8484978.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6779 a         5469 c         4283 g         8164 t
ORIGIN      
        1 tttttgaaaa ttatgttttc ttaagtcgtg aaacactttg atggatcatt tatttgtgga
       61 aaactacgtt ttgttattct aaaaaactta tataaaaaat agtaaggttt ttatttcgaa
      121 tccataaaat attcttggat tgcttctcgt gtttttttca ctttttcgct aagcttttcg
      181 tttttagcca tatagaaata aaaaatgata tcgatcgtag caaattgggc caaaagtgaa
      241 ttagttgccg cacttcgatt cgtgatttct tttggtctag atgtttgtac gagtacatcg
      301 gctatcttgc taagcgggtt atttccaaat tgcgtcaacg caatgatttc gatattcatg
      361 cttttcgcca gctcggctaa tgcgactaca tctgcacttc tacctgagtt tgaaacaagc
      421 caaagcactt tctttttctc attgctgaca agttgcggga gcaagacgtg gatatctttt
      481 tcaaaaatga ttggcttacc gacacgagac catttttgca gaatatcttc tactactaaa
      541 gaagaggcgc caaccccata taaaaaaaca gtctctgccg attccaaaca ctctacgact
      601 ttttggatcg tttcttcttc tagaatatct actgtttctt ttatcgctac ttgtgcattg
      661 ttaccaagtt tgtttttgag agagtaaaag gactcatttg cttctatatc actaaaacct
      721 tcatcgattc cagtctgtag ttctgttgaa atcgtgattt tgaagtccgt cagactttga
      781 aaaccgattt ttttggaaaa acgtacaact gtcggcgcac tcaacccgga agcttctgct
      841 aattcattag ctgtcattgt agggaccttc tcaatattac cgataatgta gttggccaac
      901 tttttttctg ctgaagaata atcttccatc atactttcaa tacgactaat tacgctcata
      961 agttcctcca cccttttaat gatagtctat gtaagtatac catatcagac ccttattcac
     1021 ggtcgtatac tggataaaaa acaaaaaaac aatccgccca acagaatatc ttatcccatc
     1081 aatcccacag attctattga gcatctgtgt ctttgaggaa tacctgttat ctattgttcg
     1141 gattgctttc tatctactac ttttttacag gaaatttacc tattagtttt ctctgctgta
     1201 atttttgact tctgcttcca tagcatcaat aaagatattc aaatcttcta cagctgtttc
     1261 tttacttcca taacgacgta cgttcactgt tgcatcttcc aattccttgt ctccaacaac
     1321 taattggtaa gggatctttt gtgtttgaga ggcacgtatc ttatagccca ttttttcatt
     1381 acgatcatca acttctacac gcaacccctt catttgcaaa cgttctttga tttcataagc
     1441 ataatcgcca tgtgcatcaa cagaaacagg gatgattgtt gcttgtatag gtgccaacca
     1501 tgttggaaaa gcgcctttgt atacttctgt taaataagcg acaaaacgtt ccattgtaga
     1561 aacaattccg cggtggataa ctactggacg atgcgtattt tccccgtctt ctccaacata
     1621 tgtcaaatca aagcgctctg gcaataagaa gtctaattgt atagttgata atgtttcttc
     1681 tgtaccaaga gctgttttga cttgaacatc tagttttggt ccgtaaaagg ctgcctcgcc
     1741 ttctgcttca aagtaatcta gttctaattc atccatagcg gctttcagca ttgcttgggc
     1801 tttttcccac attgcgtcat catcaaaata tttatctgta ttgtttggat cgcgatagct
     1861 caagcggaaa cgataatctg taatattgaa gtcagcatat actgccacca tcaattcaag
     1921 cgtacgttta aattcatctt tgatttgatc aggacgaaca aatgtatgac catcattcaa
     1981 cgtcatttct cgtacacgtt gtaaacctga caaagcacct gatttttcat agcgatgcat
     2041 cattcctaat tcagcgatac gaattggtaa ttcacgataa gaatggatcg tgtttttgta
     2101 gaccatcatg tgatgagggc agttcattgg gcgaagaaca agcatttcgc catctcccat
     2161 atccattggc gggaacatgt cttcttgata atgatcccag tgtccagatg ttttgtacaa
     2221 ctcaacatct cccattattg gtgtatatac atgttggtaa cctagactaa cttctttgtc
     2281 tacgatataa cgttcgatcg tgcgtcggat tgttgcccct tttggaagcc agaatggcaa
     2341 accagaacct acttctgggg aaaccatgaa caagtcaagt tcttttccta gtttacgatg
     2401 gtctctttct tttgcttctt cacggagacg gatatattct ttcaaagctt tcttgtcaaa
     2461 aaaggctgtc ccgtatacac gttgcatcat ttgattgttt gagtttccac gccaataagc
     2521 acctgccact gataataatt taaatacttg tatacggcca gtggatggaa catgtggtcc
     2581 acgacacaaa tcgatgaatt cgccttgttg ataagcagta atcacttctt cttctggcaa
     2641 ttcactaatc agttctactt tgtacggatc acttgcaaaa atttctaatg cttcctgttt
     2701 tgaaagaaca cggcgttcga tcggcagatt ttctttgacg attttcatca tttctgcttc
     2761 gattgccggc aaatcttctg cagttacttg gttttgtcca ttatctgtat cgtagtaaaa
     2821 ccctgaatcg atagctggtc caacgccaaa gtggatattt ggatagaggc gacgcatggc
     2881 ttgggccatc aaatgagctg ctgaatgacg gactaagcca agtgcatctt catggtctgg
     2941 tgtgatgatc tcaatacttg catcttcctc gattggacga gtcaaatcaa cgagttttcc
     3001 gttcacttta ccagctaaag ctttttttgc taagcttttt gaaatgcttt cagcaattgc
     3061 caatgttgtt gttccagact caaattcttt gactgcacca tctggaaaag taatttttat
     3121 cataatagtt tcctccttga aatgttttga ttgatcagat catcaagctg aaaaacaaaa
     3181 aaatcctagc atctcttgaa aagagatact aggacgaaaa gttcgtggtt ccacctaatg
     3241 ttcaaacgca aatgcgttcc tcaagcgttg taacggtccg accgcccttg cttcaacaag
     3301 ggtttcgaaa gtggtcatcc tttggttttc tcctggaaaa gtttcagcct agcttttcct
     3361 ctcttatcga agactctcaa attcagttgg ctttctcatc aatcaattat gtgtttattt
     3421 aaccactaat aaatagaaaa atcaagtggg aaactaaact tattttttcc attccccatt
     3481 tacaccgtat gttccttttt tcatctcatt gatgatgaca tggatgtgtt cttttggtgc
     3541 ctgcgtgttt ttatgaacag cttccgtgat atctttcatc atattttcta attgttccgg
     3601 actgcgacct tctactaatt cgacatgtac gaatggcata atgctctctc ctctttctga
     3661 tcgttctttt ttcaactcga ttctatcata ctatgtttgt cttgggtact gaatcaggaa
     3721 aaatggctgg atcttttttt gctctcaagg cactcaaaat agagaccgta tccacgactt
     3781 cttgcaaaac agctccgaat aatgcgggga ttagtcccgt acttgcaatt aacatcaaaa
     3841 aaatacaaat aaagatgcca atcagtacag actgtttcgc tattctcatc gtatctcttg
     3901 aaatcactac tgcttttgct acacgcaaga gatcatcttt caagatgacc gcatctgcac
     3961 tttcactagc agctgtagaa ccatgagccc ccattgcaat accgatatct gcagcagcaa
     4021 gtgctggtgc gtcattcacg ccgtctccta ccatcgcaac aggtctttga tctttaggga
     4081 tactttctaa aatggtcagt ttatcttgag gcaagctacg ggcatgtaca gaatctaatt
     4141 ggactttttc tgcaatcgtt tgagcaatgg cagatccatc cccggtgatc atcatcaaat
     4201 ttttcacatc aagttttctt agctccttga ttgtctttgc tgcttctggg cgaatctgat
     4261 ccgaaaatag gatcgttcct gcaaaagatc gatcaattgt tacatagatt gctgtattgg
     4321 acgtttctat ttcttcagct gaaacaaaag aggcacgtcc aatctttact tcatgattat
     4381 tgactactgc ttggattcct tgaccggtca cttctttcag ttgactaaca ggatacagtt
     4441 ggctgttttt cacgttgttc actaacgaac gagctaagat atggctagat tcctgttctg
     4501 cactagctgc atacagaagt agctcttctt tttttattcc attgactggc tgaatcgcct
     4561 ctacttccaa tatccctttg gtaagcgttc ctgttttatc aaaagcaatc gtttttgtac
     4621 gtgcgagctt ttcaatcgtt gttccagttt ttacaacgat cccgttttta cttgaacggc
     4681 tcattcctgc aactaaagca atgggcgcag ctagaatcag tggacaaggt gatgccacta
     4741 ccagtacctc agcaaatcgt aaagggtctt ttgagaacca ccaagaaata ccaccgatca
     4801 aataagcaat caacgtgaaa gggactgcat aacgatcagc taaacggaca aatcgggcag
     4861 gcttttcttt tgattcttcg actagtttaa taatcatttg atactggctg tctgcggcac
     4921 gtttttcagc gatcatttgg atcgatccgt ctccatttaa agaccctgac ataatctgat
     4981 ctccaggttt cttggtttcc gggcgggact cacctgtcaa agaagattca tcgaccgaag
     5041 attctccttt ggtcactcga ccatctgcgg gtacaagttc gccaggcttg actagtaact
     5101 ggtcatcaat atttatttca tcgattacta tatcctgcaa ttttccttct ttgaatcgat
     5161 gggcaattct tggggtgtta tctaacaaag actgcagttc tttacccgct cgtttattcg
     5221 cgtaatcctc tagactatct ccgcccgtaa gcatgatcaa tatcatcaaa ctagcccagt
     5281 attctttgac ggctaatgtt gcaaggattg cggtaatcgc taatacatct acgccatatc
     5341 ttcctgaacg taacgtcttg atcatttcca aaaacatcaa gaaggcgaga atccctccag
     5401 taaatgcgac aatcgcaaat gcaagggccg gctggtgaaa aacaaattca cttaataaag
     5461 ctatcccacc aataagcaaa gtaagtaaaa attttttgaa atgtgtcatt gttttttctc
     5521 ctcttttatg tgacttagag aggttagtaa taaatttcca gctgcctctt tcatcttaag
     5581 catagtcctt gactagcact ttttaaaaca ataggcctta attgaaaaca actatcacta
     5641 tcagcgtttc caaaattcat cagttctaca aacaaaaata ttcagcaagg attacttttt
     5701 cgattcatca gttaaaaagt accttttttc gaaaaactat gctatacttt taattcgtta
     5761 tttacggcgg aaggagcaaa catgcgatga atgagaaaca actagaacaa acgcacgtgg
     5821 atgaaacgat cgaactaatc cagatcgaac aaaatatgct ggatacaaaa caacaagaat
     5881 tgactcagga aatgaatcat tcgacaaaag acacagctaa tcacaagatt cgagctggtt
     5941 ccaatgaagc tttttatgaa tctgctgttg aatatcgcca acatgagcag gaattgcttt
     6001 tgaaatacca tactcttgaa gcacagcaaa aacgcctgca aacgttgtct gtcatgaaag
     6061 atagtcctta ttttgcccgt atcgacttta aagaagaccg tgaaacagaa acattatatc
     6121 taggtatcgc ttctttaaga gatctggcag aagaaacaat cgttattgac tggcgcgcac
     6181 caattgccaa tctttattat gaaggtgaga ttggtcccgc attttatgaa actgaaacag
     6241 atagaataga tgtcgatctg cagttgaaac gacaatttaa aatccaagaa ggacgtattt
     6301 tatctatggt agatacgtcg gaagtaatca atgatgactt tttgctagag atattggatg
     6361 aatcatcaag caaccatatg aaaaacatcg tctctacgat acaaaaagca caaaatgcaa
     6421 tcattcggga cacaacaagt aaagtaatgc tgattgaagg aatcgccgga agtggaaaga
     6481 catccgcttt gcttcaacgt attgcttttc tgttgtatca taatcgcaag tggctcgagg
     6541 ctgaaaacgt tcttctattt tcacccaacc atttgttttc tgattacatc tcgactgtat
     6601 tgccttcatt aggtgaaagc ggcgtgccta cacagacatt caaaaattat ttggtgcagc
     6661 tgctgccaga gttttcttta ctagatgaac agcaacaaga aagtggtttt ttgctagggg
     6721 aaaaagatcc gatacaagtg atgaaaagcg gattgacctt agtcgatcag attgatcgct
     6781 atatccaagg catcacaaac tacggtccct tgttccgtga tatgaaaata aatggccgta
     6841 cgatcctatc aaaggaatcg attcgccaat ggtataaaga aacaaatgaa caattacctt
     6901 tacatcatag actttctcta ttacaaacga agctcctgaa aaaattaggc ggtctgcaaa
     6961 aagacgaaac acgtcagcaa tgggtcaaag atctggcgga agaacagctc caagaactat
     7021 acgcaacaga tccaaatctt gaatatacag aacaaaagga gcgttcatta cgtaaaaaac
     7081 tggccaacca aatcgtacgt aaaagattcc gtcgaatcca tcgagggatc atgaattatc
     7141 aatttgtcaa tataacgaaa caatatctcc actttctaca aactgtgcca ctttctcttc
     7201 taacacgaca taaaatcagc gagaccaggt ggcaggaaca tttgacaact gttcgtacac
     7261 acttgcgtca aagagaattg cgtcaagagg atgctgtact attcttttta ctgatgcgcc
     7321 gaacatatcc agtgacggtt acacacaaag ctcgctatat ctttattgat gaaatgcagg
     7381 atttcgcacc atcacaagtg gcattgctgc aagccttgtt cccaaaagca aatttgactt
     7441 tctgtggaga tctaaatcaa aatgtctttg gaaatgaaac gatcgttgga tcattggaag
     7501 accttttcgt ggaccgtgaa gtcactcgtt tccaattaac gacaagctat cgctccacaa
     7561 aagaaatcac tgattttgct aatcatttta ttgtgaaaga acatcaagta gaaacaacag
     7621 caagaaaagg agaccttcct ttgctcatcc aaggaacaac agatgatatc aaattgaatt
     7681 ggttagcaga taccattcga acgactcagg gaaaagcccg gtattggcgt acagcaatca
     7741 tagctaaaac acaagaagaa tgcgaagctc tttatgctca actgccagac gagttgaaac
     7801 aacaagtcca gctgatttta aatgagagtg actttatgaa gcgttccatt attttacttc
     7861 cagccttttt agcaaaaggt ctggagtttg accgagtttt cttgtgggat gtagatgcag
     7921 aacatttttc ttctcagcaa gacaaattga tcctttacac catgtgtaca cgtgcgatgc
     7981 atgaattgac cctttttacc tccaataatg aaagtccttt gttaaacaat atggatattt
     8041 catcttatga gaaaaaagta ctgtaagtaa aaagacaact gctatcatta atgttagcag
     8101 ttgtcttttt atttacaata ataattgcag aaaaagtaat tcaatcattt aaggtcattt
     8161 attctatata taaatccaaa tctgtttcag gatattttaa tttgacaagc gaaataatca
     8221 ttttcagttc ttccatgttt tctggatatt ctgggtcaaa taccttgcta tgacggccaa
     8281 tcccgccatt gtcgatcaat cggtaaattc gattccattc aatgaattgg tcaattgatt
     8341 ggaaattgct gattggatag ccgccacaat ccaaaatctc tgatacacga aatgctaggt
     8401 atgcactatc gatcacttta ttttttttcg tgtctatctt ctcatttctt tcgataagaa
     8461 ttttttcagg acgatgtttc aagctccact ctaatagtag aaagaaagcc tccatccgat
     8521 actcttcttc aaataaccga aaggctgaat cagaacgacg aaaaagcagc ggaatcagat
     8581 aattttgtct ataaatcagc attggtgcgc ggtccaccat acactctaaa taacggttca
     8641 gataccctgt accgacatag tttcctaatc gatagccttc aacgccgaat cttgcgtatt
     8701 tttgcgctaa ttttgaacaa tcagttacgt tcacagctaa ctcttctact ttgcggtaac
     8761 cgcttaagat acgttcaaaa ggttctaaag tgatttttgc ttccatattc ttccccgtcc
     8821 atcttccaca tacttcatat catttcacat gatacttatg ctctcatcat accacaggtt
     8881 gaattagaaa agaaaataaa tcacacaaaa aagcaagaca atctgttagt gagattgtct
     8941 tgcttttcga aaaagactga atacttttcg ttaaaactgg ttgcttagtc ttctaatgcc
     9001 acattatgga acacatgctg tacatcttct agatcttcca gtgcatcaat cattttttca
     9061 aatgtttctt gatcttctgg agatagtgtt acctcgtttt gagggatcat ttggatctca
     9121 gcgattgaaa aatcagtgat tcctttttct tttaatgctt cttgtatact atggaaatcc
     9181 gctacttcac cgtaaacgat gatttgtcct tcttcttcca ttacatcgcg aacatcgatg
     9241 tctttttcca ttaggtattc aaggatctcg tcagcgtctt ctcctgcaaa agcaaagatt
     9301 gctgtattgt caaacatgta agaaaccgct ccggaaacac ccatgttccc gccatttttc
     9361 ccaaatgctg cccgtacgtc tgctgctgtt cgattcacat tgttcgtcaa tgtatcaaca
     9421 atcaccattg agccgcttgg tccaaatcct tcatagcgca attcagagta tgtttcatct
     9481 cctgcacctt ttgctttttc gattgctcgg tcaataatat gttttggtac attataagtt
     9541 ttagcgcgat caatgacgaa acgtagtttt tggtttgcat gcggatcagg atcacctgat
     9601 tttgctgccg cgtagatttc aatcccaaat tttgcataaa tccggctatt attagcatct
     9661 ttagcagttt tctttgctac gatatttgcc catttacgtc ccatattttc acttcgcttt
     9721 ctgatttgat agtttcataa cgcaaaaacg tcctgttcca ttatacttgt gaatcacgat
     9781 tctgtgaagc tttttttctt tcttccttca ttcttctcat ttttagtcaa ttatctatac
     9841 tcggattggg atttgcccga aattctgttc cggaatattg cctcccaatt gatagatttg
     9901 atcacttcta actaacagtt ccgcttcttg tgcctctttt tttccgattc gggaataaat
     9961 agaaatctct tttttttctt ttaagaatct tcttccagca tcggtaaatc caagtagtcg
    10021 aagataattt ttattttgtt ggacaggaat ctcttgcttt ttgatattca gcaagacata
    10081 acaagataat cgttggatcc gtgtccatgt atatcttttt gtttttacct ttgacacaaa
    10141 atcctcaaaa ctatcagcag ttgcagctgc tgttttcatg cgcgattcta ttccttcggt
    10201 catctgatag atatctgtca gttccagcaa agtagaagat aacaaccgat aacgcaaata
    10261 cggccaataa gattcccaag tctgcagcga acaagttgaa agatcttctt ctgtctgagg
    10321 cggcacaagt ccagctattc tttttccttc ttctaacgca tggcgaatgg ctgttgcact
    10381 ggctatcctc tgatctgtga tgattttttc atgatagcct gcacttttac gtcggatcgg
    10441 atgtaattcc atagggtgga catacttggc attttccttt gcataactta atcccaagat
    10501 atgatttggc gaagaaaaat caagcgtaat atcgggatac agactgcgaa aaacctcagt
    10561 cattttttgc ggataactat acgtttgatc agacagttct tgaaatgcct cttctatcct
    10621 atcttgattc tcttggacaa agcgtccgaa actgctgtaa tcaaacgaag tctcttcttc
    10681 tgttccaaaa cacaaactgt cacagtccag tgtttgcaag atttttactg cacctttagc
    10741 aaaatagtca gctgactgaa ccgcccattc gacgggaagc tccactacta aatccacacc
    10801 attttgtaaa gcttcattcg cgcgatgcca tttatcgatg atagccggtt cgcctctttg
    10861 caaaaaattc ccgctcatta cagcaatgac gacctctgct cctgttattt ttcgtgccat
    10921 ttccacatga tagcgatgtc cattgtgaaa aggattgtac tctacgataa tcccgcagct
    10981 tttcatgcgc atcctccttc atagttatcg ataaaaaaga aatagagaca agtaaaaaaa
    11041 ggcaaaaatc ggaaaaaata cccgaactat ataaactatt ctcgcaaaca caatacttct
    11101 gcgacgaaaa gctgcactgc aagagcaatg cctatatcgt tcggatatat aatttatgtt
    11161 gaagatttat tcttttcttg ttaactcgag caggcaaaac cgctagtctt tgtatgctac
    11221 aaagaaccag cgctttgatt tctctgatgc tgcttcgtct gtaaaatcag aaaatgcttc
    11281 gactttcgaa aagccgctgt tttccagcat tcttaaataa ctttccaatg gataggtacg
    11341 ctcttgatgt agttcatctt cacgaataaa tcgaccagac ccttcttgtt cttcaacgaa
    11401 aaaggtaaga aaatgttcga tgctgtgttt ctcgcttcct ggataactat cccacaaaaa
    11461 agcaaaatta tccccttgat aatgatagct atattctgga aaaacctcat ctacttgata
    11521 ggtggagtgt acgtcaaata aaaatcggcc attttcttct aacgcttgat agacttcatc
    11581 aaacacttgc tgtacctctt gtctattttt catatagcac agcgaatcag aaaaacaggt
    11641 aattgcctga tattgtccga tttctgataa atcgagcata ttgccttcca caaattggat
    11701 cgatgcgtct tcttcatatg ctcgctggct ggcaatcatc aacatctctt ctgaaaggtc
    11761 taaccctgtc actttatatc catcttttgc aaaggcaacc gctaatgctc cagtaccgca
    11821 tgccagttcc aaaatatttt ttgtgtcttc ttgtaaatga cgtttcgaaa aagctaacca
    11881 ttgctgataa agtgtttcgt ccatcacttc atcgtaaatg aaagcaaatg tttcataagc
    11941 catatgttaa tccaaccatt cactaagatc gactagcgga gcatctgacc atagtttttc
    12001 cagattatag aaatttcgtt ctggcgcatg gaaaacatgt acgatcacat ctccaagatc
    12061 gatcaatacc cattttccgc cttcttttcc ttcaatacgt tttacttcat atttgttttc
    12121 ttcttcttta tcaatgattt cttctgtaat tgcgttgatt tgacgttcgc tatttgctga
    12181 acagatcatg aaataatctg ccaacaagga aacatttcga acgtctaaag cgataatgtc
    12241 ttctgcccgt tttgaatctg ctgcttcaat cgcaattttt aattgttctt tactatctat
    12301 gatgatttcc tcctaatatg agcagcttat ttttttgcta cccagtaatt atatgtttcg
    12361 attgtttttg gataaatctg ttgttcttgc gcaagcaaat gggccaatgt atgttttgtt
    12421 tcaaatgcta cagcttcgtc tagatcagcc catgcaatgg ctcgtgcatc ttgtacccca
    12481 ggaaaatctc gacctggttc aatatagtct gctacataaa tgattttgtc aaggagactc
    12541 atcttagcag ccccagtcgt atgcacccgg attgcatgaa gaatctctgc atcggtgatt
    12601 cctaattccc gctctacaaa cgaagcgcca acaagaccat gccagattgc attattataa
    12661 ttcaataact ccggatcaaa accgtctctt acgattacca ttttgaactc ctcatctggt
    12721 cgttcttttg cataatcatg tgtcaatgca gcaatactag ctttttcagg agaagcaccg
    12781 tattttttgg caagagccac ggctgtttct tctactccaa gcacatgctt gaagcgttgt
    12841 tcgctcattc tcatctgtat tttttgcatc aggttttctc tagaataagc agtatattca
    12901 ttactatagt ccatattcat acaacccttc attttgaata taatcaatta ctttatctgg
    12961 aacaaggtag cggatagaac agccttcttt gatcttttga cgtattttcg tggaactgat
    13021 gtcgatctca ggtacatcta cccaaattac aggatatgga gtatccgttg tgtatcctgg
    13081 tcgacgtata cccacaaagt tgaccataga agtcagctca tcgattttat accattttgg
    13141 caaatattct accatatccc caccgataat aaagtaataa tctgtatccg gattgttttg
    13201 cgtcaattcc ttcatagtat cataggtgta acttttccca ccacgagcta attcaatcgt
    13261 ttcaatctgc aaaaaaggat tgtcttccac cgctaattcc aacatgttga gtcgatgttt
    13321 tgcatcaatc gtctgtttct catcaacatg aggtggctga taagaaggca tcaaaaatac
    13381 gcggtcaaga cctaaattcc ggccagcttg ttcagccatc actaaatgtg caaggtgaac
    13441 gggattgaat gttccgccca agatccctac ttgctttttc ttctctaaga attgaggcat
    13501 ttcttcagga aataattgtg aacgaacaaa tgtcttagct tgcgtattca tcatggtcac
    13561 tccttatatt gcttttactt cttttgatag cctttgatac ttttcttttg tggaaggttt
    13621 gtataataca ataacacgtc caatgatttg gacgatttcg caatctattt ctttttctaa
    13681 tacttgagct gcttcttctg caatctcatc tgtattttga agtaacgata ctttgatcag
    13741 ttctcttttt tctaaagctt cttcaatttg aacgatcatt gctgaattga tcccaccttt
    13801 tccgatttga aaaatcggct gaagatgatg cgctttgcta cgtaaaaaac gcttttgttt
    13861 tcctcttaaa ctcataattt tccttcttcc ttaaatcaat gctttgcgtt tcaatacatc
    13921 tacacccttt ggcgcccagc ccgcaattac tactggcgct gttacagtga tccagcctag
    13981 accggcaaaa acgatgtctg ttttttcttt cacagaaaac tcaaaacgaa caagctccgg
    14041 aaatgcctcg acttcatcag gacgaggagg ctgtaataat ccacctacat gtttttgata
    14101 aaattcgtct gctttttcca gtttcgttcg atgaatattt acatcatttg acacataagc
    14161 aataaatgaa gcacgattgc cgctgataaa atcaaatctt gcaagtccgc ccaagaaaag
    14221 tgtttgccct tcattcagct gataaacttt aggtttgatt tcttttaacg gtgcaatgat
    14281 ccgcaaatct tttttaccta agtagtgcgc catttgatgg cgatggataa ttcccggtgt
    14341 atcaatcaaa aaatgcccgt catctaaagg aatctcgatt ttatccaagg tcgttcctgg
    14401 aaactgcgaa gttgtgatta catcttgtac tcccgctgta tttttgatga tttgattgat
    14461 aagcgtcgat tttccgacat ttgttactcc gacgatatag acatcccgtc catcgcggta
    14521 ttcttcgatt ttttctaata gttccgtcat ctcatgagat ttcttagcac tagttaataa
    14581 tacatctact ggacgaattc ctgcttcgta agcacgttcc ttcatccatt gtgttaattt
    14641 agtttttttc aatgatttag gtaatatatc cactttgttc ccaactaaaa gtactggatt
    14701 gtcaccgaca aaacggtgta gtcctgggat tagtgaacca ttgaagtcaa aaatatccac
    14761 cacattgacg atcaatgcat ctttttgccc taattcattc agtaagcgca aaaagtcgtc
    14821 atccgtcaat gagacatctt gggtctcatt ataatggcgt aatctgaaac aacgctgaca
    14881 atagacttcc ccggaagcta aacctttttc caaagccgtt tgcggcgtgt atcctaattc
    14941 accgggtttg tttgtttgta taacggcacc acagccgata cattgtaatt cttctgtcat
    15001 tcgattccac ctcgccagcc catatcaggg tgttttgcta atagataggc cataattctt
    15061 ttttcccaaa aacgattaaa ttgtgtcttc caactatctg ttgcgataat gggttttaca
    15121 aggatgctac gtatccctgc acgattggct ccacgtatat cagtcataat ctgatcgcct
    15181 accattacta tctcatcatc tgctaaatcc aattgtttct ttgctatgtt gatccctctt
    15241 gctaaaggtt tcattgctcg tgcaacatac aaaagatcaa atttttcaat cgctcgcgcg
    15301 acacgttttg aattattgtt tgaaacaaca acaacaggga ttcctgcatt tttcatttct
    15361 aaaatccaag taagcaactc ttctgttcca tctggattat tccatgcaat caacgtattg
    15421 tctaaatcgg tcaatactgc tttgatgcct agattcttta attgggcagg tgtaatctga
    15481 tagatggctt ctaccatcca tgttggtttg taatttgaaa acatgctgtc tcctcatctg
    15541 caaaaaatga aggagcacat aaagtactcc tcaaccccgt attatactgc atatcagcaa
    15601 aaaaaactac ctctatactc gttcgtcttt cagaaaaagc atttctttac aaaatgacca
    15661 aaaataaaga ttgatcacag aaaaatttag aaacaaaaaa caactaatat tggcggaacc
    15721 tcttgtatcg tttttccagt aatttatgct gatctaatcg aacaagttcc gtcaatcttt
    15781 caatcaacgc ttttttcata atgtgtgcga ctttatatcg tggcaactgc tcgccgttga
    15841 acgtttcagg aataactcga tcaacgattc ctaactcgcg tagttctggc gcagtaattt
    15901 tcatcaactc agctgcttct gcggcgcgac taccgtcttt ccacaaaatc gacgcaaatc
    15961 cttctggaga aagcacagca taaatcgttt cttccagcat ccagacttca tccgctaaac
    16021 ctagagcaag ggcaccgcca cttccacctt caccaatgat gatggcgatg atcggtacct
    16081 ttaaatcaga catttcgagc agatttctgg caatagcctc tccttcgccg cgctcttctg
    16141 ctcctacacc gcaatatgca cctgctgtat tgatcaaggt aatcaccggt ctattgaact
    16201 tttccgcttg tttcatcagt cgtaaagctt ttcgatagcc ttcaggatgg ggagaaccaa
    16261 aatttctgcg gatattttcc tgcagatccc gtcctttttg aataccgatc actgttaccg
    16321 gtttgccatc taatcgagcg attccaccaa caatcgcttc gtcatctgca aataaacgat
    16381 caccatggca ttcctcgaaa gaatcgaaga tctcttcaaa aaaatccaag cttgtccatc
    16441 gttctttagc tcttgctaac tgaacgacat catgtgctgt cttatccacg atctatccat
    16501 cctttctgtg tatgcaattc aagaagcgta tggattttct gtttcaattc catgcgggga
    16561 acaatctgat cgacaaaacc atgagataat aagaattcag ccttttggaa atcttctgga
    16621 agttcctgct tgatcgtctg ttcgatgacg cgtcttccag caaaaccgat caagctttgg
    16681 ggttctgcta gaataatatc gccttccatt gcaaaactgg cagttactcc tccggtcgtc
    16741 gggtccgtta aaacagttag ataaaaaaga ccttcattgc tatgccgttt gacagcagca
    16801 gagatttttg ccatctgcat tagagaaaag attccttctt gcatccgtgc gccacctgaa
    16861 gctgtgaaca agacgactgg aagactttcc tttgttgctc gttcgaataa tctggtgatc
    16921 ttttctccga caatcgttcc catacttccc atgataaaat taggatccat cactccaata
    16981 atgaagggaa tcccctgaat ggttgcttta ccagtgagga ctgcttcatg aagtcctgtt
    17041 ttatcttgca tttttttaat tttttccgta tattcgggaa atgctagcgg atctttggtt
    17101 acaagatccg tatcccattc ttcaaaactt ttttcatcaa taatcaaagc tagacgctgc
    17161 catgcaccga ttcgaaaagc gtatccgcaa tgagggcata ctttttcttc accgatgtct
    17221 ttcgtataaa ggatatgttt gcaatttgga cactttgccc acatgttatc aggaacagat
    17281 ggcgagttag caggtttgct ctctctatta ggattgatcc gaatatattt ctttttctta
    17341 aacagagcca tctatgcgcc tcctatctcc gtttctggtg tccaattcgg taaaaattct
    17401 tcttgtaaaa atgctgtgct gtaatcacct gctatgactg caggatgact aatcaaatcc
    17461 atctgaaatt ctgcgttcgt gatgattcct tctgtgataa attcacttaa ggctcgctgc
    17521 atcttcatta atgcttcaaa acgcgtattt ccatggacga ttatttttgc aatcattgaa
    17581 tcataatatg gcgggatcgt atatcccggg taaactgcac tgtctactcg cagtcccatc
    17641 ccacctgctg gaagcagtaa gttttggatc ttccctggag aaggagcaaa atggaaagca
    17701 ggattttctg cattaatccg gcattcgatt gcgtgtcctt ggaatactac ttcttcttga
    17761 cacacattca aaggctcacc ggctgctatc tccacctgtt tttttactaa atcgattcct
    17821 gtgaccattt ctgttactgg atgttccact tggattcgag tattcatttc catgaaataa
    17881 aaatcgcctt cttcatccat cagaaactcg atcgttccgg cattttcata attgactgct
    17941 ttagctgctc gaacagcagc gtctcctagt gcttctcttt ttgtttgtga gatcactaca
    18001 gaaggagatt cttccagtac tttttgattg ttgcgttgta aagaacaatc tcgctcgcct
    18061 aaatgaatga catgaccaaa tcgatcacct aagatctgaa cttctatatg gcgagcagga
    18121 taaatgattt tttccaaata catatcatca tttccaaatg ccgctgctgc ttcttgctga
    18181 gctgaagaaa aatgctgtgg gagctcttct tttgattgaa ctttccgaat gcctttcccg
    18241 ccaccacctg cagctgcttt cagcatcact ggataaccaa ttctgtctgc tatttccaat
    18301 gcttcttcta ctgtagataa tacaccagta cttcctggaa tgactgggac atttgcttct
    18361 tgcatcaatt gacgcgcatt gatcttattt cccattgcat cgatcgttgc acttttagga
    18421 ccaataaatg tgatattgca ctcttcacac atggaggcaa atcggctatt ttcagataaa
    18481 aaaccaaatc ctggatgaat tgcttcggct tttgtcacga ttgcagcgct cagtacttcc
    18541 tgaacattca gatatgaatc aacggctttt gccgggccaa tgcagattgc ttcatctgct
    18601 aactgtgtat gaagtgcttc ggcatctgct tctgaatata tcgctacggt ttggatccct
    18661 agttcgcggc atgcacgaat gatacgtaca gcgatttcac cgcggttagc tatcaatact
    18721 ttagaaaaca tatttcacct atccaatcat aaaggttaat tccgcttcgg ctactttttt
    18781 accgtctact ttagcgattc ctttgcctat tccggctgaa gctttcactt tcaatatctc
    18841 aacttcaagg tataaggtat ctcccggtgt aactttctta cggaattttg ctttgtccaa
    18901 tccgccgaaa taagccgtct ttcccttgaa ttgttcaaga gaaagtaatg caactgctcc
    18961 tgcttgagcc attgcttcaa caatcaatac ccctggcata acaggttctt ctggaaaatg
    19021 tccttgaaaa aagggctcat tgatcgttac atttttctta gcaacgatcc gttctccttc
    19081 aaccatttct tccactcgat caatcaaaag cattggataa cgatgtggaa tgatttcttt
    19141 tatttcttgt atgttcattg ttttccctct ttcctgcttt gcttattttt gctgaatcct
    19201 aaagagtggc tgtccatatt cgacaactga ctcgttttct acaaggattt ctgtgatcac
    19261 tccgtcggcc tcactagtta tctcattcat cagtttcatc gcttcaataa tgcagataac
    19321 ttctcctttg gcaacttgat cacctacttt tttgaaagct ggtttgtcgg gcgctggttg
    19381 cagataaacg attcccacga ttggcgaaac aacctcgatt ccatcaaata ctgccgattc
    19441 tttttctttc attatttctg gagcagttgt tgcttgttca ggtatgacat tgactggctc
    19501 cgaaactggc aacgtttcgt ttgtttgaac catattcatg cctcgacttg ttttattttt
    19561 attcatatac aattcaaatt gaccttcacg caaatcgaat tccgttagcg aggattgatc
    19621 aaattgactg actaattctc tgatttcatt gatatccaaa attattcctc ccagcgtttc
    19681 atgcacagaa ccgcattatg tccgccaaac cctaatgaat tactcattgc ataagtaaaa
    19741 cttgcaggtt tgctttcgtt tttgacaact gtcaagtcaa tctcaggatc ttgtttttct
    19801 acattgatcg taggtgggat aaattgatgt tctaatgcca gcaaagttgc caaggcttcg
    19861 atcccgccgg ctgctcccaa taaatgtcca gtcatcgatt tcgtgctgct gacgtaagta
    19921 tctctgaaat tttcacctaa tgcgatttgg attgcttttg cttctgcctg gtcattggct
    19981 ggtgtacttg ttccgtgagc attgatatat ccgacttgtt ccggctgtac accagcttct
    20041 tccatcgcaa gcttcattgc tttagatgca ccagaaccat ctggagtagg agctgtcata
    20101 tggtaagcgt cacagtttgc accgtatcca acgatttctc catagatttt ggcaccgcga
    20161 gcttgtgcat gttctaaaga ttcaagaact agaacgcctg ctccttcacc catcacaaaa
    20221 ccattccgat tttcgtcaaa cggaatcgaa ccacgattag gatcagttga ttctgagagt
    20281 gctgttaaag ctgcaaatcc agcaatcccc atctcacaaa tcgttgcttc tgttcctcca
    20341 gcaagaatga catcagaata gccatgtttg atgttacgga atgcttcccc aattgaattc
    20401 gttccgctag cacaagctgt tacgatagct gtacaaatac cttttgcacc tacacgcaaa
    20461 gcaatatttc cagctgccat gttggcaatc gccattggta cgaacattgg agaaactcgt
    20521 tttgcacctt tttcatgtag acgaataact tggttttcga ttgttggaag accaccgatc
    20581 ccagatccga tgatgacgcc aaaccggtct tgattcattt tttctttatc taaaccgctc
    20641 atctccattg cttctaaagc agcgtagatt ccatagatcg aaaacatatc cattcgtttg
    20701 ctatcttttt taataaaata tttatcgtaa gggaaatctt tgacttcacc cgctaaggta
    20761 atccctgttt ctgaagcatc aaatttagtg atcggtgcga tgccattcgt cccctctttt
    20821 aagctattca aaaagctgtc tgcgtcattt ccaataggtg aagtgactcc atagccagta
    20881 attaccactc gattcatttg tttgctctcc ttttcttatc cgtgcatgac caatccgcca
    20941 tcgacattga tgacttgtcc ggtgatatat ggattttgtg ctaagaaaac tgctgtttgt
    21001 gcaatatctt ctacttgtcc gaaacgctgc atcggaattt gcttttccgc ttgttccttg
    21061 actttgtctg ctaaaacttc cgtcatatca gttgtgatga atcctggcgc aatggcatta
    21121 catgtaatgc ctctagttgc agcttctcgt gccacagatt ttgttaatcc tactacgcca
    21181 gctttactag ctgcataatt tgcttgacca atatttccaa tcaacccaga tacactagag
    21241 agattaatga tggccccttc tcgctgcttc atcatttttt tcaatacgtg ctgcgtcata
    21301 ttgaaggttc caatcaaatt gatatccaga cattttttga aatcttctgc atccattcgc
    21361 atcacaagtt tgtcatttgt gattccggca ttgtttacta acacatggat cgatccaagc
    21421 ttttcttctg cttctttgat catttgtccc gctttctcat aatcagagat gtcaccggaa
    21481 actcctacgc attttacacc gaatgcttcg atttcttcta tcttttcttt tggaatctct
    21541 ccgcgaccat tcaaaatgat atttgctcca gcttttgcaa aagctaaggc catcgctttg
    21601 ccgattcctc ttgtactacc tgtgataaat acattttttc cttttacatc cataacgttc
    21661 ctccttatag tattgctatt gtttcatcaa atgtctgctt attctcaaca cgcagaactg
    21721 gtattgtttt atcgattttt ttcataaatc cgcttaatac ttttccgggg ccgacttcga
    21781 tgacttgttc gatgccgatt gtttttaact tatggatact ctcatagaaa cgaacaggtt
    21841 gcatgacttg ctgttcaagt agtcccgcga ttgtttcttt cttcattatt tccgttgttg
    21901 tgttgcttat gataggaaaa gaaggttcag agaattgaat ctgacttagg tccttcgcca
    21961 gtttttttgc tgccggttct aaaatagctg tatgaaaagg cccgctgaca tttagaggga
    22021 tcattctttt gaaccctttt tcttttaata gtaaaacagc ttcgtctact gctttttctt
    22081 cgccaccaat cacgatctgc tgaggtgtat tataatttgc aggagatacg atgccgtact
    22141 tgctagcttc atgacagctt tcctcgatcg tttcaatcgg cgcattcatc actgcaacca
    22201 ttttcccgct tccagctggg gcagcttctg tcatataagc gcctcttttt gctactaacg
    22261 caactgcttc agtgaaggat agcgcattac tagctactaa tgccgaatat tctcctaaac
    22321 tcagtcctaa agctgcatct gggatgattc catgctcctt caataagcga taatacgcga
    22381 tgctgactgt tagaatagct ggctgtgtat attgcgtctg atccaaacgt tcattttctg
    22441 taaagcaaag ttcagccata tcgtagccga gaatctcact tgcttcatca aatgtctgct
    22501 ttacaacggc ttcttcatat aaatcttttc ccataccttg atattgtgca ccttggccac
    22561 taaataagaa tgctgttttc atgtttttct ccaatatatc cagaatatat gtttgattct
    22621 tcctattcaa aaaggccgag acattcctat ttcaggaaat gtcccagccc ccaacctaat
    22681 tttaatgatt atgcagtttg tttttctacg taagtcacta aatcttgaac cgttttgatt
    22741 ccgtcttctg tttcgatttt cacgtcaaat tcatcttcaa tttcattgat gatttggaat
    22801 aaatccaagc tgtcagcttc taagtcttct tggatgtcag tagtcaattt gatttcttca
    22861 gtttcttttc ctaattcttc tgcgatgatt tcttgaattt tttcgaatac cataataata
    22921 tattcctccg atattgtttg ctttttattt ttataattta acagtctaca actccatgag
    22981 aatacttccc catgttaaac ctccgccata gcctgtaaat attactttct gctgttttcc
    23041 taaggtgatc tttccactag aaactgcttc atccaataag atagggatgc ttgcagcaga
    23101 tgtattccca tatttatcca tattggtcag gaacttttct tgcggcatct ttacttttcg
    23161 ggcaattttc tcaataattc tgaggtttgc ctgatgcaaa agcagataat caactggttt
    23221 gtctttcatg acttcttgga tattgtttgc aacgtctcgt acggcaaaat caaaaatatc
    23281 tctgccagtc atttgcaaat aatacgaacc ttctgaatct tctgaataga acggattttt
    23341 attttgctgg tagccagcgg tcaatgagct acttctggta ccgtcagctg ctaatttttc
    23401 tttcagcaat tttttctgtg gattcgcttc taagagaatc ccacctgctc catcgccaaa
    23461 caacacagca gtcgtacgat cagaccaatc gattagctta gatagtgttt caccaccaat
    23521 caccagtccc ttagttgagc cgcagcggat cattttttca gccatgctta acgcataaac
    23581 aaatcctgag cacgcagcac tgatatcaaa agcgatagca tgagttgctc ccaatttccc
    23641 ttgtacttgt gctgctacag atggtgtcgt gtaatcgggc gacattgttg ctactaaaat
    23701 aaaatccaaa tctttagggt ttagattttt ctgttttatc aatttttccg ccactaaaat
    23761 gcataaatca gaagtctcct gatttgttac acatcttcgt tcacgaatac ccgtccttga
    23821 cgaaatccat tcatcagagg tatccatcat cttggaaaga tcattattag taataatctt
    23881 ttcaggaagg acgctggctg ttgcagtaat cattccaaat tgttccatta aatatcctca
    23941 cttatagtct tgcaagaagt cgtgcaagtt tttcagtgca tccagcaagg catgctgttc
    24001 ctccgtagcc attccgtcta agatgttctt gaccatttca cgatgaaaat gctgatggac
    24061 ccgaaatagt aactttcctt ttttcgttag tcccaacttt acaacgcggc ggtcatcttc
    24121 actgcggata cgttcgacat agcctttttt tactaacttg ttaatagcag tggtcaaggt
    24181 cccaacagta attgatagtt ctttggctac ttctgaagtt gtttttttct tgtacatccc
    24241 gattgcctcg atcgtgtgca tctctgtgat tgacagatct ttgaattgag attttttcag
    24301 ctcagactct tcgattgtca aaatatcatt gaagacacta acaagataat catttactgt
    24361 ttttaaatct ggttccatgt tccaccgtcc actaattagt ttgattatca aatcttttga
    24421 tgttcaaact atatcttaac ttttttcaaa atgcaacctt aaaagatcat ttttttacta
    24481 tatgagcttt ttttaagatt ttactcattt aaaacaactg ataaatcagc atttataatt
    24541 tcttttatta gaaaaatcat ctatctcaca tattttgatt atcaaagtat tttctgtgaa
    24601 cagaactata gaattcccta aaacttgtcc attatttcaa aatttgttat agtcttttta
    24661 ttcaaagcgt aaaaggagat cctatgttaa aagaa
//
LOCUS       DACDEG010000030        24574 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.030, whole genome shotgun sequence.
ACCESSION   DACDEG010000030 DACDEG010000000
VERSION     DACDEG010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.030"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(153..551)
                     /locus_tag="IV142_001310"
     CDS             complement(153..551)
                     /locus_tag="IV142_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP8484979.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(548..1321)
                     /locus_tag="IV142_001311"
     CDS             complement(548..1321)
                     /locus_tag="IV142_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP8484980.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1318..2001)
                     /locus_tag="IV142_001312"
     CDS             complement(1318..2001)
                     /locus_tag="IV142_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP8484981.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2168..3055)
                     /locus_tag="IV142_001313"
     CDS             complement(2168..3055)
                     /locus_tag="IV142_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8484982.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3052..3921)
                     /locus_tag="IV142_001314"
     CDS             complement(3052..3921)
                     /locus_tag="IV142_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8484983.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4058..5344)
                     /locus_tag="IV142_001315"
     CDS             complement(4058..5344)
                     /locus_tag="IV142_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8484984.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5617..6195
                     /locus_tag="IV142_001316"
     CDS             5617..6195
                     /locus_tag="IV142_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8484985.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6195..7124
                     /locus_tag="IV142_001317"
     CDS             6195..7124
                     /locus_tag="IV142_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8484986.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7121..8857
                     /locus_tag="IV142_001318"
     CDS             7121..8857
                     /locus_tag="IV142_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8484987.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="IV142_001319"
     CDS             complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="IV142_001319"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP8484988.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9753..10262)
                     /locus_tag="IV142_001320"
     CDS             complement(9753..10262)
                     /locus_tag="IV142_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP8484989.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="IV142_001321"
     CDS             complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="IV142_001321"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP8484990.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11563..12654)
                     /locus_tag="IV142_001322"
     CDS             complement(11563..12654)
                     /locus_tag="IV142_001322"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP8484991.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="IV142_001323"
     CDS             complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="IV142_001323"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP8484992.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="IV142_001324"
     CDS             complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="IV142_001324"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP8484993.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="IV142_001325"
     CDS             complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="IV142_001325"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP8484994.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16617..17591)
                     /locus_tag="IV142_001326"
     CDS             complement(16617..17591)
                     /locus_tag="IV142_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP8484995.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17572..19455)
                     /locus_tag="IV142_001327"
     CDS             complement(17572..19455)
                     /locus_tag="IV142_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP8484996.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19473..21698)
                     /locus_tag="IV142_001328"
     CDS             complement(19473..21698)
                     /locus_tag="IV142_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP8484997.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21813..22847
                     /locus_tag="IV142_001329"
     CDS             21813..22847
                     /locus_tag="IV142_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8484998.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22914..23927)
                     /locus_tag="IV142_001330"
     CDS             complement(22914..23927)
                     /locus_tag="IV142_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8484999.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24005..24193)
                     /locus_tag="IV142_001331"
     CDS             complement(24005..24193)
                     /locus_tag="IV142_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP8485000.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7061 a         5342 c         4397 g         7774 t
ORIGIN      
        1 agcatatttt gaaaagaaag tctgatgctt agagtagaat ttcataaaaa tttactattt
       61 cataagtatt cacgagaaaa atacaaacaa aaaagcggaa ataacgctca tccgccttct
      121 tgtttgtgtt tatttcctct tcaaacgctt gtctatgatt cagacttttc tctatcgtta
      181 ttttcatcta aaagttcttg cattccgata atttctcgat attgatgaat cttagtgata
      241 ccaatgactt tgtctacatc aatactaaaa caaataccat tacctggaat atcgatgttg
      301 atgccttgac caatcgcatc cattacttta ttcgtcaaac tatcgggtac taatgtcaac
      361 acaatatctt tttctggcga aatgtccaaa cctaaaaatt tccctgaatc atgtacacca
      421 gaacctcttc catgtaagat cgttccgcct gctgcacctg cttcttttga aaagtcgata
      481 acatcttcgc ccattcctcc gtcaacgatc gtcacaatca tgtgaagatt caatgctaca
      541 agatctttca tttgctttcc cccaattttt gcaatctttc tttttcgatt ctgtcttttt
      601 catcttttcg tttataaata tatcctagta ctaaagtgga taatattggt gtcaatgcaa
      661 tcattgcaac cacaccaaac ccgtcaatga tcggatctct tccttcaatt tgtgcagcga
      721 ttgccacgct cattgataaa ataaatgtcg aacacatcgg gcccgttgct actccaccgt
      781 tatcaaaagc cattgctaaa aagatcttgt ccacttttcg tcctaaaata aatgctagcc
      841 cgtatcctgg gatcaagaaa taataaagag agaatccgac gagcaaacgc cacatagaca
      901 atcctactgc tgcgccgatc ccaattgaaa ttaccatcag catgattttc gatcggattc
      961 tcccctcgct caattcttca acctgtttga tcattacatg aatcgccggc tcagcaaacc
     1021 ctactaagaa ccccattaaa aagccaaggg gaaccagtat ataattgtaa ggcaatcccg
     1081 caatagcact gcctaaatgc tgtcctaccg gtacataagc catattgact ccgtgcagga
     1141 aaaggatcaa cccaatagtc gttatcaaga aacctttcat aatgtcaaag aaacgctttt
     1201 tgcgcatacg aaaactaatg atattcatta aaacaaatag gattacgata ggtgccactg
     1261 ccaaaatgac ttccatggta acgatttcga ttccatctat cacttgctgc cagaaactca
     1321 ttggaagatc acccccaaaa tcatcacggc aagaatcggc cctaacgaag caataccgac
     1381 cattccaaaa ctgtcatttt catttttctt ttgtttgatc atactagtca tccctccggc
     1441 aagggccaag ataaatggaa cagttactgg accagtagtc actccccccg aatcaaaagc
     1501 aatcggcata aattcggaac tggtgaaaaa tgaggcgatc aaaacgccta tgtagcctat
     1561 agccatcaat tgataatagg atagcttgaa tacgacccga agcagcgcaa aggctagaaa
     1621 tactccagtg ccgacgctga cgatcccaat cagcaacact ctgccaatct ctccgtcaga
     1681 tatatcatat acttgttgtc ccaaaacttg cacggaaggt tcagcaatcg tgatcacgat
     1741 cccgatagca aatcccaaac tgaccaaaac ggtcaaactt ttctttttga tcatatattt
     1801 ccctactaga tctccgactt ccatcataga ataatctgct ccaaacaaaa agagcgtcat
     1861 tccgatcatc atgattgccg caccaaccag aaatgaagcg aggtcttctc cttctaacgg
     1921 tgcaaaaaca aacgttaaaa tgacaatcag gacagtcatc ggcaatatag cagcaatcac
     1981 ttctttaaaa ttttttagca tgtatcctcc tcctttactc tttaagttta ccttaaaatg
     2041 aaagagtttt cgagcacaaa cttaaaacgt tatcatttaa tttttctcaa cataaaataa
     2101 cacacgtccc aaaagtatct ttttacttgg aaaaatgact ttgaaggaac gcgcttattg
     2161 tgttttttta ttttacagag ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa
     2221 atacgatggc agttggtaaa gtagcgatga ctaatccagc catgatcatt ccatagtctg
     2281 gtgtatagga agcacccata gcagataaaa caagcggaac cgtccgttta tcaggagatt
     2341 gcaaagctac taagggccat aaataattgt tccagctact catgaatgtg atgattgcag
     2401 ccgcggcata ggtattttta gccgtcggca tatacactct aaaaaaaata cttagttctt
     2461 ttagaccgtc taatcgagcg gcttctacta gatcttttgg aaaagattta acattttgtc
     2521 gaaagaagaa gatcaaaaaa gcagtgctaa cagatggcag gatgacaacc aagaaactgt
     2581 tgattcccaa aaccgttccg ttcaattgtg aaaacattcg atataaaggg atcatcaatg
     2641 ccgcaaaagg aaccatcatc gatagcagta gaatattgaa aattaggtct tttcgtttac
     2701 tttgataaat ctcaaatcca tatcctgcca ttgacgaaat cagcaatgca aacacagtgg
     2761 tcaccaaagc gatcatcgct gaattccaaa gggaacgagt aaaattcagc tcattggaaa
     2821 acaaattttg aaaattgatg atcaagttac tgccaatctt tatttttccc gatgtgatat
     2881 ccacgggtgt attcgtcatt cccaaaacca tccagacaaa agggaaaatg gaaataaatg
     2941 ataggatgct taatgctgta tactttaata ctgacccaat tgtcgaacga agttctaatg
     3001 cttgtttttt gacgttgggg cgttcgattg tttctttttc taccatattt ttcatcgact
     3061 ttctcctcct actttcgttt ggattatcga caatatcaca ataaaggaaa caataacaaa
     3121 ggaaactgct gccgcatatc caaaattagg cgtgaattta taactcaaat tatagatgta
     3181 ttgagatagg gtggtcgttg catttgctgg tcccccgcca gtaatatttt gtacttcatc
     3241 aaaaagctgc aatgtcccga tcgtcgaagt aatggaagta aacaaaatga ttggttttaa
     3301 atttgggatc gtcactttga taaactgctg ccatttagaa gcaccgtcaa tagatgctgc
     3361 ttcataaatc tccgaatcaa tattttgcat gcctgaaagg aaaaaaatca tattgtatcc
     3421 agtccaacgc caagtgatcg aaagaatgat cagaactctc gcccaaattg gatgagtcaa
     3481 ccagccaatc ggtgtgtcaa tcataccaat gcccaacaac aagttgttta caaggccaga
     3541 ttgagaaaat agacttttca tgatcagaga ataactgacc atcgaagtga tacatggaag
     3601 aaagatagcg gttcgaaaca accctttata tttcaacttt ttatcattta aaaggttgga
     3661 aataaaaagt gctagaaaca acatgatcgg tacttgtaca agtaaataca ggaatgtatt
     3721 aaaaaaagct tttttaaaag ttgcatctgt caacatccgc tgataattac ctaacccgtt
     3781 aaatgtcata ttgacagggg gaccactttg aaaagacgtg atcaaagcca aaaccatcgg
     3841 aataaaaacc atgattgata tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt
     3901 gaataaatct ttctgcttca ttcttttccc tccaattcta tcattgatta tctacatgaa
     3961 aagacaagaa agacaatatc tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct
     4021 gatgaaaaag cgaagaaatt cactttttta ccaccgatca attagccaat tgtgcttcta
     4081 cttgtttttg tgtatctgct agtaccttgt ctgtctcttc tccattgatg attcgatgaa
     4141 gagcttcagc tacgactgct tcaattgcat aggtctcatg tccgtaattc acttctggta
     4201 tttcagatgt ccatgttgaa aaatcgtcaa agatcttttg accactgtaa aattcagaag
     4261 cttcttgata agcttcttgt tcattcgcgc taagcatcgt agatactagt ccgatttctt
     4321 tcgcaagagt gcccatcagt tcttcattcg aagcaaacgt tgcttctagg aaatctttcg
     4381 ctgaatcttc tccagctact cctttgatca cataccagcc agcgccgcca aggttagaag
     4441 cttgcgcttt ttgcagattt tctggaagtg caggtattgg tgcaatcttc cattttccag
     4501 attggttttc tgctccttgg atcgttgaag aataccaagc gccagttggg ctagaagcta
     4561 ctgctcctga ttgtatcgca gttactccag cattccagtc agaggtttgt tcgactagat
     4621 cttctttcaa aagagtggca aacaattcaa gaccatactt taaagatgca ttgtcttgga
     4681 tcgtgactgt ctccccatct tcagatgtat accattctcc tgcctgttgc ataatcatcc
     4741 ttactcgacc aaggtcagaa ggattgactt cagcgatttt ctttcccgtc ttttctttga
     4801 catctcgcgc tacttgaata tactcgtccc acttcagatt ctccatatcc tcttctgtat
     4861 aacctgcttc tgccatcaga tccgtacgat agaagtttgc tgttacgccc gaatcgaaag
     4921 gaacaccgta tattttatct cctactttgt ttaccgcaaa tttatacggt gcaaaatgat
     4981 cttcctttac aatagcagac aaattagcga atgcatctgg ataagaagtc aaatagcctt
     5041 gtatcctata atcttcaatc aagacgatat tgggcaatcc ttctgtattc ccgctcgcta
     5101 aagcggtgtt cagtttctga acgatatcat cctgagacat cgtaacaact tctatctctg
     5161 tttcttcgtt ctcataaact ttttttgctt cattcactgc tttgatattg aatgtctcat
     5221 cccacgccca gacagtgact ttttcttctt tgtctgctgt actactagca cttccgcatc
     5281 ctgttaagaa acctgatgct gccgctaaac aaacgactcc agcaattaac tttctgatgt
     5341 tcatttttat ccgctccttt tccatccatt agttgtttac gtttgctaca ttcatcatac
     5401 caaaatttga aaacggtttc tttattattc agatacttca aaaatttctc ctaaaataat
     5461 aaaaaaatac aaatcaacta tgacaaaagg aataatctta gcatctagac caaagatcga
     5521 aatggtgagg tttctttaaa ataaaaatat ccttgcattt tctcctagaa ataagtattt
     5581 ttaaattaca agactattct taaggcgggt aaaaaaatgc caatacgcgt aattcttatc
     5641 gatgatgaac agacgatttt agatggaatg aaacagttga tcaattggtc tgagcacggg
     5701 tttcaattaa tagggacttt ttccagtccg ctgaaagcgc ttgatttttc tttggtacat
     5761 gcaatcgata tcgtggtcac agatgtaatg atgcctgaat taaatggaat cgaattatcg
     5821 cggctattaa aacagcaaca acctgaaaca cagatcctta tcctctctag ctatgatgaa
     5881 tttgatatgg ttaaagattc ttttaaagaa ggcgtcgctg attatctttt aaagcctcga
     5941 ttgagtcctg atttttttct aaattcctta actgccgttt cacaaaaaat caaaaaaaag
     6001 cccaacaaaa gcccaatcac caatcgttat gagcagatct gcgagaatct ttcacagcag
     6061 attgctggac aaactcgtcc attgcttgac cctcttcttt ttgaacacac cttgtttttc
     6121 tgttatatac cgaaaaacgg ccattcagtt caaaacaagg ctctaaacaa aaacagtcga
     6181 tcacccattc attgatggat agcgacgatt cgtttactat ttatcctgtt tctacctttt
     6241 cggaagaatc cggctatgta atcaatactg attccaaaga tacacttagg aagtttgttc
     6301 aaaagtttcg tcaagaagtt tttccaggtg atttttttgt tgtttctgat tcttttgcaa
     6361 tccaagatat caatgaaata ttccaagaga tcagaaattg caccaaagga caacgtttct
     6421 atcataaggg aaagaacctt gcttatcagc atgaacttct tccgttatta accaaccacg
     6481 agcagcaagc aagtagttat ttaacaaaaa tcgttcataa agactacgct ttagctgtca
     6541 aagaacttga gaatttcttc acgacgatat tggaacagcc tattgatcca gcttatttga
     6601 aacatgaggc aatcaatcgt ttctatgcat tactcaacgc tttggctgac gagcatgttg
     6661 ggaatgaaga actttctttg ctcaagatcg cccttccttc tttattagcc gatgctgagt
     6721 atttcgaaga tttttctatt cttgtcaaag aaacacttga gaaactcacc cacttttctg
     6781 caaatgcaaa aaaagacaat ttcgatatct tgactttgat ctacgagttc gtccaggaaa
     6841 attatgagaa agaaatcaat ttacaacttt tatccgaaaa atatcatttt tcatatagct
     6901 atctctcagc catctttact gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag
     6961 tccgtatcag taaagccaag ctctttttga cggaaacacg gcttactttg acggaaatct
     7021 gtgagaaagt cggctatacc gaactgggtt atttctccag agtttttaaa gaagagacag
     7081 gaatgacacc ttctcaatac cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga
     7141 gcatgaactc tttgccaaac ttctgttcat tttgttcatc ggtctttttc tgcaagccat
     7201 cgtcatttct ttgtttattt atcatcgatc aagggatgcc tatatccagc tgttcaatca
     7261 atcgaatgat gtggtactaa aaaagattca atctgaattt gaaagtttga atgatacaat
     7321 cgagaataca ttagccgctt ttgattctaa tccagctgtc aaaagctatt tttccaatga
     7381 tccagcccaa catatggaac agcttcagca actaagaaca atccaaaaaa tgaatgactc
     7441 tctttcaaaa atccatccaa tgatcgatta cgatgtattg atctttggag aaaatggaag
     7501 aacttttgtc ggaaacgaca tgctcactgc cgtatctgcc gattcttttt ttcaatctgc
     7561 gattgctcag agagtgaatg aacgtgctgc tgatacacaa atgctttttg cacaccacgg
     7621 attgactctt cgcgataaga aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac
     7681 attgaatcac gtttacggat atgctgtttt atcgatctca tccaagcaac tagcgaatct
     7741 ttttcaatct gtagtaaatc ctgagatatc gaagatctcc ttcatgaacg aagaacagac
     7801 gatcatcgca tcaaatgaag aaaatacaat tgggagccga tcaatgattt tcaaaaaact
     7861 aacagtaggt gaaacaacga tttcaaatgg acagcagttg acccgtcttc ctctgtaccg
     7921 ccaggattgt acattactca gtcaagtcga tcttcattct ttatctaacc aaatgggcgt
     7981 gatttttccg attattttgt ataatatcgt gacacttgct tttgctggct cattggtatt
     8041 tatgtatttg gatcgacaca caaaatcgat ctaccgcttg attgattcgc taaaacggct
     8101 agaggaagat ggcttaaaag ctactgttcc tgtacaagga agccatgaag tccaattgct
     8161 agggaatacg atcaatcaat tgctaagtaa tgctcagcat cattatgaac agacgatcca
     8221 agacgaaaag aagaaacgtt cgcttgaaat ccaaactatg caagcacaga tacagcccca
     8281 ttttatttat aatacgctga ctagcttgaa atttttgatt tggcagaaaa aaacagatga
     8341 agcagttcaa ggaatcgaca gtttcattac tttactaaga agtacgattg ggaaaaaaga
     8401 agaagtgatt ccagtcaagg aagaaatcaa aagcgtacaa agttacatcg atcttttgag
     8461 tatgcgatat ggcgaaagaa tctcggctaa aataatgatt cctgatgaac tgcagtcatt
     8521 atctatgccg aatatgatcc ttcaaccaat tatcgaaaac gcctatcttc atgcttttca
     8581 caataaaacg caaggattca tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt
     8641 cgaagtgatt gataacggcg atggcttcga tactgaaaat accaaacaaa aaaatgatca
     8701 tttttcagga atcggcatca gccatgtaga tgagcggata cgcctaatgt atggcgatgc
     8761 atatggctta acgatccaat cagtgattgg gacaggaacg actgtaacga tttgtctacc
     8821 gcttcttcct aaaaatcaat cagaatatca aaaataaaga atagccaagc gctgattttt
     8881 ttattttaag ttaaataaat caatcagtat tcttgacttt catctacgcg tctcttctac
     8941 tgtcagcact ggataacagc ccaatatttg actttctcct ccgagcatct tgatttcttg
     9001 gagcgcattc tctactaatg tcattggacg gtccaacaac aaatcgatca cgaaaaaata
     9061 ttctcccaag ctagttttca atggtcggga ttcaatctta cttaagttga tttcccgcca
     9121 gccaaaagct gccagcattt tatgaagcat ccctggccga ttagcaggta gtgtcaagat
     9181 gacactcatt tttactggag ctgactgctg gctggtcatc tttcggtcac cgacgatcca
     9241 aaaacgtgtc tggttcaact cattatcttg gatattctca gctaagatct ccaatccaac
     9301 atgctgtgct gtttctaatg aagcaatcgc tgctgcgtct tctttcggat gttctgctac
     9361 atacattgct gcagcagttg ttgattctgt tgctactaaa ggaacattag gataatgcgt
     9421 ttctaaaaac tgttgtgatt gtgctagtgc ttgtggatgc gaaaggattt ttgtaatctt
     9481 tgttgctgga ttgcctaaaa gctgctgctt gataggaaga acgatttcac ttttaacttc
     9541 gacttctgga tgtttggaaa gaagatcgat tgtgtgatga acagctcctt ccaatgaatt
     9601 ctctacagga acgacagcca gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg
     9661 gatggaagca tacgcagtca attgttctgt aggaaatagc tgacaagccg cctgataagt
     9721 gaatgaactt tctggcccta gatagctgac gttcatatgc ttttgatcct ttctataatg
     9781 gtatcgatga tcttttctgg tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct
     9841 tcataccaag aaagtcgttg tgccaataat gcttttattt cttctcttgt ttttccaaga
     9901 gctaaaggtc gaatagacgt ttcatcttgt tggatacgat caactagaca gtccgcttct
     9961 gcctctaagt aaagaacaat cggctgtgtt tttaaaaaac gccgattttc tgcactttgg
    10021 acaatccctc caccagtcgc gataattttg tcttcatttg acagctttct taaaatatct
    10081 gactccactt ttcgaaagct tttttcaccg taatagtgaa aatattcggc aatcgacaat
    10141 tggatatgtt cctcaatctt tgtatctaaa tctacatact ctttttgtaa acattgagct
    10201 aaccctttac cgattgttgt ttttccagcg cccatgaaac caatcagcaa aatgctagcc
    10261 atgagcttct cctcctgaca caagttctgc taaatcgtca aaaaatgccg gataagagat
    10321 tgaaacagct tcagctcctt ctagttcgca aggtccatca gctatcaaag cagctacttg
    10381 cagcatcata ccgattcgat gatcgcctct gctggacact tttgcgccat gcagaggtgt
    10441 cggcccatgg atgatcagac cgtctgctgt cgcttttatc tttgctccca ttttctgtaa
    10501 ttcttctgcc accgcatcaa ttctgttggt ttcttttact tttaattctt ctgcgtcttt
    10561 gatgatcgtg attccttctg cttgagtagc cactaaagct agaatgggca attcatcaat
    10621 cagacgcgga atgatttctc caccaatcgt tgctttcttc aaatgactcg tcttgacact
    10681 taaatcagcc gattggttct gttcattgtg attcgtctgg gtaatcgctg cgcccatctc
    10741 ctccaacaca tctaagatac cagttcttgt tggattgacc ccgacatttt ttaacaacag
    10801 ttggctttcc ggaacaagaa gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc
    10861 tggaacagtt atctgttgac cagttaattt ttgcggtccg gtcactatga tcgttttatc
    10921 ttccactgtg atccttccgc caaattgacg aatcatttct tccgtatggt tacgggaacg
    10981 ttctttttca acgattacgg atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga
    11041 tttgacttgc gcactggcta caggcatatt gtatcggata ggtgataaag agcgggttcc
    11101 tgtcacagtg attggtagat actggtcatt ttcttctcct tgcaagtcag cgcccatctc
    11161 gcgtaaaggt tccatcactc gccccattgg acgtttagac aacgaagcat ccccgaacag
    11221 agtagtcgta aaaggctgtc cagccaaaat acccatcaat aaacgtatgg tcgtaccgga
    11281 gtttcccatg tcaattgatt cggttggttg gacaaatgag tggctaccgc gaccatgaat
    11341 cttgatcacc tcttcctctt cttcgatctc gacacctaat tgttggaagg catgtaaagt
    11401 actcagacaa tcttctgcgc gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc
    11461 tccaaacatg atgctgcgat gggaaattga tttatctgct ggtatcctaa cagtccctct
    11521 taatccatgt atttgttgta ataattgcat ttatccagcc tcctatttga tccagcaatg
    11581 ataactggta ttagacataa tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa
    11641 actgatttgc agtattccta aaatatcttc cctggtctct tggatcttca aattaatcaa
    11701 agaaactccc gcttttccga tcagtccagt tacttctgca ataacgccag gttcatctgg
    11761 cacatcgata aataaatcaa aaaaggcagg aatcgcgcct tgcttatgga caggcaactg
    11821 gtctcgactt tcttttgccc gattgaaaaa agaaaagatt tccgattggt tctcagtcat
    11881 gatccattct gctatttgtg tcatttcttg ttgccaatct gccaatcgtt ccaataatat
    11941 ctgcttattg ctcaacaaaa tatctgtcca catgacagga tctgaggaag cgatcctagt
    12001 gatatctcga aagcctcctg ctgccagttg tttggctcgc ggatactctt gattgaattg
    12061 ttctgcctga ttgactaagc cagctgccag aatatgtgga aaatgactta gcattcctgt
    12121 tatacggtca tgttctcttg ctgtaagagt tacatatttt gctttggttc cgcaaaatag
    12181 ttcttgcagt ttttcaacat cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc
    12241 attttcaaat agtttagcat ctgctgcctg tatacctgat ttatgagaac cagccattgg
    12301 atgtccaccg acaaaccgaa gatttttctg ctcagcggat gtcacgactt gctgcttcgt
    12361 actgctgaca tccgtaatga tgacgtgatc agacaagttg attctttcaa ggacatcaag
    12421 ataagctaag gttgtacgga cgggaacggc taataagata acgtctgctt gttctgcacc
    12481 tgtttcaaaa tcacttgcaa cgtaatcaac gatctgctgt tctactgctt gctcacgggt
    12541 ttttgactga ctatcccaac caactactat cagttctgga ttggtctgac gaatacaaag
    12601 tgccatagaa gcaccaatca gtccaagccc cacgataaaa acatgttgtg ccatcttatt
    12661 tgcccccttt ttagtaatgt ttcaagtagt cacggtactc cttcactgca gctttcaact
    12721 caacaagcga atcacttggg aattttgaca atagctctgt cgctaccaca gtggctacaa
    12781 cattttccgc aacgacgcta gctgcaggaa ctgctgtact atccgaacgt tctacacttg
    12841 ctttgtaagg ttcttttgta tcgatatcaa cactttgaag tggcttgtac aatgtaggaa
    12901 tgggtttcat gaccccgcga atgacaagtg gttcgccatt ggtcattccg ccttcgaagc
    12961 cgcctagatt attggtacgt cggaaatagc ctctttcttc ttcccatagg atctcatcca
    13021 tcacttctga gcctggtaat gaaccagaag tgaatccgac cccaaattct acgcctttaa
    13081 aggcattgat actaaccaca gcttgtgcga ttttggcatc tagcttcgtg tcccattgta
    13141 cgtagcttcc taatccagct ggaacgcctc caacgagcac ttctacaatt cctccgatcg
    13201 tatctccatt tttctttgtc tgatcgatca aatcttttat tctttgctca actgctggat
    13261 caacaactcg tacttctgac gcttcagaaa tttctttgat ttcttgaatc gagatatttc
    13321 caggtatttc tgcttggatt ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa
    13381 gttcttccag aattttcttg gcaattgcgc cgattgctac acgcatggtc gtctctcttg
    13441 cagaggaccg ttcaagcaca ttccgaagat ctcgatgatg gtatttcata ccgcctacca
    13501 aatccgcgtg tcctggacgc ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca
    13561 cttctgctac agacatgaca gttgtccaat ttttccaatc tttattttct accactaaag
    13621 tgacaggtga accaattgtt ttgccgtgac ggatgccaga cgtgatccga acacgatctt
    13681 tttcgatctt catccttcct cctctgccgt aaccagcttg tctgcgagcg agttcgtgat
    13741 tgatttcttc ttctgacaga ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt
    13801 ctggtccatg ggattctccc gcagtcatat aacgcatctt acagtcctcc taagtaatct
    13861 ttgaatgtct ctgtgggaac aggaacgatt tttgcttgcc caatcttttc tagcaagatg
    13921 atattcaatt ggcttcctct tgctttctta tcatgggtta tcgcgtggaa taattcatct
    13981 ggatgactta gatcgtacgt aatcggcaga tggaattttt gaagtatcct ttctaaagca
    14041 gctgtcgatc cagttggaga caagccgatt ttttctgcat gagaagtgat ggctaccatt
    14101 ccaatagcta ctccttctcc atgactgact accccatatc ctgctgtatt ttcaattgca
    14161 tggccaattg tatgaccaaa atttaaaata agtcgatttc cttggtcaaa gacatcttct
    14221 tccactactt ttcgtttgat ttccaacgta gcagtaatga ttggaacagc atgttccaga
    14281 agatcttttt cgtctttcag ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct
    14341 gctgatttga tgatttcagc gattccttct cgtaaacgac gtaccggcaa agtttttaga
    14401 acctcaggat caatcaatac accagctggc tggctaaatg ttccgacaag gtttttcgct
    14461 ttgtttgtat tgactgctgt cttcccgcca atactagaat ctacttgtgc aagtagcgta
    14521 gtcggaattt gaacgaaatg gatcccacgc atgaacgtcg aggccacgaa tgctgctaga
    14581 tcacccacca ttcctccacc aagtgcaagt atcccatcgc tgcgggtaaa atcattatct
    14641 gctaaaaaat cgtataactc agctgcttgt tctaaagatt tactggtctc acccgccggt
    14701 acgattgaaa ggactggttc aaaattagct tgtctcaatt ggaacatgat tttttctgca
    14761 tatagtggag caacgtttgt atcagtgatc acagctactt tttgaggtct ccaaatcgtt
    14821 tgaagccatg atccagcctg ttctagtgcg cctttttcaa tatgaatatc gtatttttgg
    14881 ttatcaacat gtacagtaag cattcgtttc acttctttct tttaaaaagc tggaattaat
    14941 cgagtgtctg aatcttttcc attgcttctt tcatgatcgc aacttctttc atcatgcgtg
    15001 agtaagtctt ttcattcaac gattggggac cgtctgacca agcatttgca ggatcaggat
    15061 ggatctctac gatcatgcca tctgctccgc ttgccacacc cgcacgagcc attggtggta
    15121 caagttccca aattcctgta ccatgacttg gatcaacaat cacagggaaa tgagttaatt
    15181 ttttcatcaa tgggacagca cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc
    15241 gaatcccacg ttcgataaaa atgacaggag atttgtcttc gactgcaatg tattctgcag
    15301 cattaagcca ttcattgatc gtaccggaaa ttccacgttt caatccaaca ggtttacctg
    15361 ttttaccgac agcagatagc agtttaaaat tttgcatatt acgtgcgcca atctgtagga
    15421 tatcgctgta ctcagccacc atatcgatat gcccttcatc catcacttca gtaatcactt
    15481 tcattccaaa ttcatcagcc gcttgacgga tgtatttcaa tccttcttct tctaaacctt
    15541 ggaaagcata tggagaagta cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc
    15601 cggctttggc cattcgcgca cattctctga tttggtctaa tccttcgata gaacaaggac
    15661 cagccatcgt cacgaaactg ccgtcaccaa tttttactcc gtctacatcc acgactgtat
    15721 tgccaggatg gaattctcga gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt
    15781 ctactccttc atagcgtgta aatgctcctt cttgtaaact tcgcgtatca ccttttagtc
    15841 caatcactac tcgttctgtt ccttgatcga ttgagacttt gaatccttct ttttcgatat
    15901 gatcgacgac tttttttatt tgttcttccg ttgcttcttt tttcataatt actatcattg
    15961 tttttcccgc tttcttttgt ttacttatct atcttggctg attttctcta ctttttcaag
    16021 gtggttttac attaattcct actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt
    16081 ctttcctact tatgacccag ataatcaaca aaaaaagatg tccctgtagg aaattgcctc
    16141 tacagtgaac atcttcaacg agccttaaaa agcgctgcaa aaatataaaa tagccactat
    16201 agatgaattt ctctacgtgg ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac
    16261 aaacctggtt tgtttgtacc cacgaagtca tgaatacaaa cctatctaaa atagctaaag
    16321 taatacgtat tcagttttgt aagttttcta gatttcataa aaaatcgctc caaactgatg
    16381 taagaattgt acctagtata gacataaaaa aatcgactgt caatcatttt tatgaaactt
    16441 tttttaattt tctgatagtt aactttgttt ttcagaaaaa taacaattgg ttttattgaa
    16501 gaaaatagtc atgaacaaaa gtatctgaga acttttttac tctcttgttc atgacatata
    16561 gaataaagca gctttgtctt gtaaactccg tttttcaaat aataatctaa agattgctac
    16621 tgaataataa agccgatttc atggtcctct tcgctgctga ttcggtaagg tgactcaaca
    16681 tccgctcccc agctattgat acccccgact cctcttactt ttgcaaaaat cgtaagaacc
    16741 gttcgtcttt ctggcggtag ttcttctata tgtgttgcct gttccagttc ttctgctttg
    16801 aatggtaaag cggaaaaagc aaatggcgta tcctgctgtt caaatcgcaa cgaaaaatct
    16861 tcttgatctt tatccgtatt atccaacgtt tgatttcttg ttagttctac ccatttcgtt
    16921 tccatatgca tgccgcattc ttggggaaca aggtatgggg aaacggggag tcctgccact
    16981 ttataaatac cttcgacacc accagccatc cgatccggat atgtttcccc tgataggcct
    17041 tcatactcaa aaaattttgc tttcgttggc ataatgaacc ttaaacccaa tgcgggtaat
    17101 tccggtaatc ctttttgtcc ataataatgc atattgacaa gcattttacc ctctccagcg
    17161 atttggtagg aaattgtcac ttgggttttt ggtactgtgt tgatttccag tgtaaaaatg
    17221 atttcaaccg tttcagcgta ttcattactt gaatattgat tatttactgg agcaatcggc
    17281 aaatcgatct ttttattgtc caccagtact tctatttctt tgacttttgg atacaatccg
    17341 gctcctaacc acataccgga tttaaatcca aatccgctac ctctgtcatt atcggtcgtt
    17401 gctcgccaaa aaactggaag tgtttcccgg tatagccatt cttttccgtt tttattgagc
    17461 gattccagtc cgcctcttgt ataagaaaaa atataatgcg tattccccga tttgacacct
    17521 aatgtacagt cgccgaagac gattgcaact ttttccgtat ccatatgact tttatttact
    17581 gtatttttca taataatatc ttacctcctg catggcgggt ttttcttgtc catctgcaaa
    17641 aacgatccca ttccctgaga attcatagtc acttggttta tcgtcaaagt caccgccata
    17701 gcgcatcact tctctgcctg tgattggatc agtcacttgg atcgcctgat ctttaaaatc
    17761 ccatatatag cctccttggt acatttcata acgatcaagc aacgtcatgt aagaatccat
    17821 tccacctaac gaattcccca tgtcatgcat atattcacat aagatgaacg gtttttccgc
    17881 tttattttca aggtagtcaa tgatctcttc tggtggcgca tacatgcagc tttttacatc
    17941 tgaaatcgaa tcttcgaatg ctggatttgg aaaattccct tcataatgga caagtcgatc
    18001 ggggtctact tttttgaagt aatcgttcat ttgtcgaatg ttttcttccg cataagattc
    18061 atttccaaga gaccaaaaaa gaatacttgg atggtttttt aatgtttcga aattcgtttt
    18121 tgctcgatca agaacagccg cctgccactg aggtactgca cctggtacgt tgtaagaagg
    18181 ctcaaatgcg cccatttttt gccatgagcc atgggattcg aggttcgttt ccgccatcac
    18241 atggattcct gcttggtcgc ataaataata ccaagtgatc tggttggggt aatgagatgt
    18301 ccgtacgccg ttgatctttg cattttttat gatagccaaa tctttttcca tatcttctat
    18361 agtgatggcg cgtccttttt tcggatgcca ctcatgtcga tttaccccgt tgataatcat
    18421 ccgttttcca ttgaagtaga tgattttatc tttgatttcg attttacgaa aaccgaagga
    18481 atagtcaata tactccgtta ctcttttttc ttcgtctatc gtctccaatt caagcttata
    18541 taaattgggc tgaaaatgcc cccacagtgc cacattttca aatccatcga tgcgaatcgt
    18601 catttgctct tttaacggat attgctgttg atagatttcc ttcccatcag gatctagtat
    18661 cgctacattc agctgactgt ttttggctac ttgctcaaaa gacaaatcta cttcaagttt
    18721 gccattttga catttgtcat ctaagcgagg tttgatccaa agatcttcta aatggttttg
    18781 tcctcgagca actaagaaga catctctgaa gatcccaaaa aatcggaaaa agtcctgatc
    18841 ttccaagaat gcagctgtac ttcttttgta aacctccaca gctagaatat tttcttcttc
    18901 caaataaggt gttaaatcaa attcagacgg agtgaacgaa tcttcggcat acccgataaa
    18961 atgaccattc aaccaaataa acattgcttg ttctacacca gcaaaatgaa tcgagatttg
    19021 ttttcccttt agaccttctt ctaatcgaaa agtcgtcaaa tacgaaccaa ccggattgtc
    19081 tttagcagct gaaaagatcc cttccgcctc tttctcaggg cttgtcgtga aagggggacg
    19141 gcgaaataca tgtccctccc acggatacat cgtattgatg taatggatct tgtcataatt
    19201 tgctaattca atatgactag gaacagtgat ttcatcaaaa gaacaggcat caaaatcttt
    19261 tttataaaat gtttttggtc gtaccgaagg atttggcgaa taacaaaaac gccatgatcc
    19321 atttaaagac tgtttaaggg aggatgtggt ttctacttct tcccttgttg cataacaaac
    19381 atgatcgcta tgtgcatttg attggttcac tctaaaaact tgtggatcat ctaaccattt
    19441 tatatttgct ttcatacttt tttccttctc tctcataact ttttgaaatg aagcagttct
    19501 gtttggaaat caccttgaaa atcggggaag taaagtccaa ctgtcatcaa ttcggccccc
    19561 gaaaatatcc gcttcgaggt ctgttcctga taaagtgcct catcatctaa ccccatcaga
    19621 tatacttcat ggaacgctgg ttgagctgaa gctagtatcc tccctaaaaa cacaatagct
    19681 tcatcttgtt taggagacac aaacatccat gcggcaatat tttcttcaaa aggactcgcc
    19741 aaacgataat aatggccata ctggatcacc ggacgaatcg tttgatagga tataatttgg
    19801 tcttttactt gctttttttc ttcaggagac agctttgtca aatccagttc ataaccaaaa
    19861 acagcgctca tagctgtttc tcctcttgtt ttcaacgaag tagatctgtc ggtctgatga
    19921 tttggtacag ctgatacatg agctgtgatg gacgaaatcg gataagcaag gcttgttcca
    19981 tattggattt tcatacgctc cactgcatcc gtgttatcac ttgtccatga ctgcggcata
    20041 taataaataa acccggcatc aaaccgtcca cctccaccag aacagccttc ccataagatt
    20101 tccggataat cagttgtcaa atgttcaagt agttcataaa gcccgagcat ataacgatgc
    20161 gctacttctc cttgtctctg tgggtcaagg gtaatcgaat agacatctga aagcgaacgg
    20221 ttcatatccc atttgatgta gtcaagagga atcgtatcta aaatctttct catttgtttc
    20281 tcaatcgtct gccgaacatc taaccttgta aaatccagta catgttgtga tctggaagca
    20341 gaaggcattc tacctggctc ctgcataaga aaatcaggat gtgttcgata cagttcagaa
    20401 tcaaccgaga tcatttcagg ttcaaaccat aaaccaaatt tcaatccttt gctatgagca
    20461 taatctgcta tttcacgtaa tccatttgtc aacttgcctt catactcgaa ccagtctcct
    20521 aatgaagaat tgtccgcatc tctttttcca aaccagccat catctaatac gaacatttcg
    20581 atgcctagct cctttgcttc atcaatgatc gccttgattt tttcactatt gaagtcaaag
    20641 taagtggctt cccagttatt gaccagaatc ggccgttcag caaattgatg acgacctctt
    20701 gcgattcttt cacgtagcaa ctcatggtgg atctgactca ttttgttcaa tccctgactt
    20761 gagtaagaaa ggatcatttc aggtgtttga aaagactggc ctgcattcaa ttcccacgaa
    20821 aaacgatggc tgttgatccc accgacgact ctcaattgat tgatctgatc tttttcaagt
    20881 tcgaaagaat ggttgccgga ataaacaaat tgaagtccga tcgcttctcc agtattttct
    20941 gtcgtatgat gatgaactaa agcaataaag ttattcatat ggtgactgct cgttcctcga
    21001 cggctttcaa atacttttgt tccagataaa acagattgcc gtgaaatctg tctttcattg
    21061 acatgtgctc ctggcaaagc aatgacttca tcaaatcgcc tagtatgggc aaagtctagc
    21121 tggaaactag ccgctttttc tagtttgatt gtttctccgg tttcatttct tatctttaca
    21181 gatcgtgtga tcactgctct atcagtaaat atggtataga acaaatcgaa ataaatcgct
    21241 gtttctctat ccttaagtga aatcaccaac gtttgtgcct ctgacttgtc catcacataa
    21301 gtttgaggca agccttcgat ctctggcttc ccatctgtaa tataatggct gtcatatctc
    21361 agatccagta agtctgatcc attcttgcgc tgaatgatcg atgcgggcgt tcgaaagtcc
    21421 atgctgccat gagaagagta ttcttgcggt agtgtatctt tcgacagaga ccggtcagga
    21481 tttaagggaa catttcctga aaatccacga tctcttcggg gataattttt attgtctttg
    21541 tactgcttga ctggtttgcc aaaataaata tgcgccaata cattgctttc ttctatcttt
    21601 atcagataag aaagaaatgt atttcgtaaa tggaagactc ggctcttttc atcaaataag
    21661 atgaaatttt tatctgtttt tctatgcgta atatccatca tttcactccc aacaaaacgg
    21721 aaaccgttta ctttttttta taattcctgt atactgttta ctaaacaatt ttgtcaataa
    21781 aaatttttta tttttttgag gagtgtgtaa aaatggttgg aattagagat gtcgcacgaa
    21841 aggcaaatgt ctctcctgct acagtttccc gtgtgttgaa taacgatcag acgatccatg
    21901 tggctcagac tactcggcaa cggatagtag aggcagctgc tgcgttggat tatgacatca
    21961 gtactcgtaa atatatcaaa aagagaatgc cttcgattgg tgtgatctct acgatcggca
    22021 aagcgagcga agaacaggat atctattaca aacatttgcg agtagggatg gaagaagaag
    22081 ctcgacgtct gcatttaggg atgaatcgaa tctataatct atctgataat cctaaacaat
    22141 gggaagatct agatcagtta ggtgcggtga tcgttgttgg gactgttacg aaagaatcga
    22201 ttaccgcctt gttaaaacaa aacatgcaaa tagtcgtcgt ggataaccct gacatccagc
    22261 aagaggtcga tatggtgtat gcagatcttg aacggatgac taaaaccgta ctggaattgt
    22321 tcttagaaaa tggtcataaa agaatagcct atatcggggg atataaagtc gatatggatg
    22381 aattcggtgg gaagcagatt tctataaatg aaaagaggat gcgtgcctat cagcatttta
    22441 tgatcgatca tcacttagaa aaatatatgt cttatcatct aggtggctgg acagaaaaac
    22501 aaggtgaggt acttgcgact aatctattag aaaatgaatc ccctcttcct actgccattc
    22561 ttgtcgggag cgatccaatg gcaattggtg tctatcgggc attgaaacaa aaaaatatcc
    22621 aaattggaga agagattgtt gtagcaagct ttgataatat cgagacaatc gcaaacctct
    22681 cccctggctt gaccagtgtt caaatcaatg cgaaatcaat tgggcaagca gctgttcgaa
    22741 tggcattgga gcgaattgac ggtatacggg acgaaccaat cattttgact tatcctgcac
    22801 gattgattgt tagggaaagt tttatcccaa gaacaaaaag gtattgaaag agaggttgtg
    22861 aaaagccgct tcgtttcttc atactcaaaa cgaaacggct ttttcacaac ctcttactta
    22921 tacttattca caaaatccaa tgtctgctgg taacaatttt gcgattcttt tagcgtgtaa
    22981 tcaaattggt attcatgggt aatctctttt tttgtatctg tataaaacaa ggattgcacg
    23041 ggtaccttta attctgtcaa ccgattttct aatgcttgac cttgctcctg gaatgagtat
    23101 gcatttccgt cagttatata cgttggcgga aattccttag aaacatggtc agccacacta
    23161 gcttctttca attgctcact cgttttccaa tcttttgttc caatcaacga ccacgcaacg
    23221 gttttgacga aaaacttcat aaatcgatca tccgaatgct gctgtgctgt tagcttcaaa
    23281 tcaacaggtc cgcaataaga aatggttcct ttcagactat cttttggaag tagctgtttg
    23341 attttcattt gtttagcata cgcatcattc gtttgtatag ctgcatattg caatgcgatt
    23401 tgtgaacctg cactatctcc accaaaaaat aattgttcca tgtctagcaa tttgtcgtct
    23461 ttttcttcag ctaataaatc tttgatcagt tcattcactt gaagaacttg attagggtat
    23521 tctgaatcag gcgctaattc ataattcatc gcaaccactg cgatgttggc gtctgaaact
    23581 aattttgtcg caaattcttt gacacccgat ttatctccgc cgacgaatcc tccaccatgt
    23641 acccaaaata gaacaggaac aggccccttg ctgttttttg gatagtagat gtcataagta
    23701 ttctctttat acttggactc atacgtcaaa tctgtttttt tcgccacgtt tttttctgct
    23761 tgatcgaacg tttttttatc agtgatttga acagtatttg aaaacatatg gttgatcaag
    23821 aatgctccag gtctggggga aagctggaag gcaagaaaga ttgcaaacgc gatcccaaga
    23881 atgattccga agaaccatcc gataaattta agaattttct ttttcataag gctccctcag
    23941 tagttagtat taagcattct gattatacca tagtgttatt tcctatcaag ctctcctaga
    24001 tgatttaatg attttcccca aacttcttct gcaatgcttt tgacaaaaga aattttttcc
    24061 attgttcttc aaacaaaaca ttttcttctt ctgttgaagc aaatccacat tgaggactta
    24121 agcacaaacg atccaaaggc agatatcgtg tcgcttcgta gatccggtca atcacttgct
    24181 ggtgctcttc catttcgcca tttttcgtta cagtcaaaat ctaagacaac taatttatct
    24241 tcagagactt tctctaaaag aacaaaactc ccagcacgtt cactatcgta ttctaaataa
    24301 taggcacgga cattttcttg agtaaaataa tgggtctgct acgtcgtcat cccccctgat
    24361 gccgccaaaa tcgaataata atttccgcgt caaacatgta tatttagtgt tgaatcattt
    24421 gagaatatga aaataaaaat ctttttgatc aatcagttaa tcaaattgaa ataacgaaaa
    24481 atctgagaca gaatgacgtt cattctgacc cagatttgat tttgcacaac tcaacagtcc
    24541 ttattggcaa atcgccaaaa taatgtagac taac
//
LOCUS       DACDEG010000031        24393 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.031, whole genome shotgun sequence.
ACCESSION   DACDEG010000031 DACDEG010000000
VERSION     DACDEG010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.031"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            299..1960
                     /locus_tag="IV142_001332"
     CDS             299..1960
                     /locus_tag="IV142_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8485001.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2030..2377)
                     /locus_tag="IV142_001333"
     CDS             complement(2030..2377)
                     /locus_tag="IV142_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP8485002.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2461..3402
                     /locus_tag="IV142_001334"
     CDS             2461..3402
                     /locus_tag="IV142_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8485003.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3403..4446
                     /locus_tag="IV142_001335"
     CDS             3403..4446
                     /locus_tag="IV142_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8485004.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4452..5522
                     /locus_tag="IV142_001336"
     CDS             4452..5522
                     /locus_tag="IV142_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8485005.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5512..6465
                     /locus_tag="IV142_001337"
     CDS             5512..6465
                     /locus_tag="IV142_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8485006.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6477..7487
                     /locus_tag="IV142_001338"
     CDS             6477..7487
                     /locus_tag="IV142_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8485007.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7772..8593)
                     /locus_tag="IV142_001339"
     CDS             complement(7772..8593)
                     /locus_tag="IV142_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP8485008.1"
                     /translation="MNRFASKEEEKLYYEHEASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8690..8968)
                     /locus_tag="IV142_001340"
     CDS             complement(8690..8968)
                     /locus_tag="IV142_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485009.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9236..11245
                     /gene="metG"
                     /locus_tag="IV142_001341"
     CDS             9236..11245
                     /gene="metG"
                     /locus_tag="IV142_001341"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP8485010.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11724..12311)
                     /locus_tag="IV142_001342"
     CDS             complement(11724..12311)
                     /locus_tag="IV142_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485011.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12486..13808
                     /locus_tag="IV142_001343"
     CDS             12486..13808
                     /locus_tag="IV142_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8485012.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13963..14739
                     /locus_tag="IV142_001344"
     CDS             13963..14739
                     /locus_tag="IV142_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP8485013.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14732..15301
                     /gene="rnmV"
                     /locus_tag="IV142_001345"
     CDS             14732..15301
                     /gene="rnmV"
                     /locus_tag="IV142_001345"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP8485014.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15294..16178
                     /gene="rsmA"
                     /locus_tag="IV142_001346"
     CDS             15294..16178
                     /gene="rsmA"
                     /locus_tag="IV142_001346"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP8485015.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16629..17258
                     /locus_tag="IV142_001347"
     CDS             16629..17258
                     /locus_tag="IV142_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485016.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="IV142_001348"
     CDS             complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="IV142_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP8485017.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18700..19122
                     /gene="mgsA"
                     /locus_tag="IV142_001349"
     CDS             18700..19122
                     /gene="mgsA"
                     /locus_tag="IV142_001349"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP8485018.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19445..19981
                     /locus_tag="IV142_001350"
     CDS             19445..19981
                     /locus_tag="IV142_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP8485019.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20021..21784)
                     /locus_tag="IV142_001351"
     CDS             complement(20021..21784)
                     /locus_tag="IV142_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8485020.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21787..23502)
                     /locus_tag="IV142_001352"
     CDS             complement(21787..23502)
                     /locus_tag="IV142_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8485021.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7898 a         4420 c         4927 g         7148 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc
      241 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
      301 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
      361 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
      421 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
      481 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
      541 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
      601 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
      661 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
      721 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
      781 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
      841 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
      901 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
      961 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
     1021 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
     1081 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
     1141 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
     1201 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     1261 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     1321 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     1381 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     1441 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     1501 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     1561 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     1621 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     1681 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     1741 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     1801 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     1861 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
     1921 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
     1981 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
     2041 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
     2101 agctgtacat accgtagctg gcagacgaga gcgaggaaaa ttctggtttt ttctttcggt
     2161 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
     2221 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
     2281 attgaatgag tgtaaaggaa tggttgatga ggttttttat taaaataacc agaatcagta
     2341 gaaaagctac tgcgtagggg atagattttt ttttcatttg attgctcctt tatttttggt
     2401 tcattatcta ataatgtagc gaaacatctg tcagaagtca aaagctaggg ggaaacatta
     2461 gtgaatagtt atatcaaata tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg
     2521 ttaattgcaa caatcacctt tttcttgatg caattgctgc cgggcacgcc atatacgaat
     2581 caggaacgtt tgagtcctga aacaatcgag atgctaaata aacaagtcgg tttggataaa
     2641 ccagttatcg tacaatatgg gatttatctt tctaatttgc ttcaaggaga ttttggaatt
     2701 tccttccaat ttaaaaacca gccagtcgca catttgttag ctggaagaat cggaccatcc
     2761 ttgcagttag ggttacaggc aattatcttt ggaacagtcc tagggacgat tttaggaacg
     2821 atctctgcca tgaagcaaaa tacatgggca gatacatctt ctacactcgt agcgatatta
     2881 ggtcgttcca ttccaaactt tgtatttgct gttttattac aatatatctt tgcaatcaaa
     2941 ttacaagttt tgccaatcgc aaaatgggat ggctttgtgt acacaatctt accaacaatt
     3001 gcgttggcca tgtcgccgct tgctgactct gctcggttta tccgtacgga aatggtggaa
     3061 gtactgcata gtgattatgt ggaattagca aaagcaaaag gattgagccg ttgggaaatc
     3121 gcctttaaac atggtcttcg aaatagtttg attccattga tgactttatt aggaccgtta
     3181 gctgtagcat tgatgaccgg atcactggtg gtagaaaata tctttgctat tcctggtatc
     3241 ggggaacaat tcgttaagtc aatcacaaca aatgattatc ctacgatcat ggctgtaacg
     3301 atcctttact catttatgct gatttttgtt attttgatcg tggatctatt gtacggtcta
     3361 gttgatccaa gaattcgtgt ttcagaaggg agccgaggat aaatggaaat gataccaaag
     3421 cgttatgaaa cagttgcaga tatccctgcg gatgaatttc ttccattaca aaaaaatacc
     3481 gaagaagaac gtgaacagat tactgcacct tctttgaatt ttatccaaga ttcatggcgc
     3541 cgtttaaaga aaaacaaagc tgccgttatt tcaatggttt tactattggt catcattttg
     3601 atctctgtga tcacaatctt tgtttctcct catgatccaa ctgcccaaaa cgtggcttat
     3661 atcaatttgc ctcctcgtat tccgggaatc aatatcgatg gattgaatgg aaaagcaatg
     3721 gttggtggtg aacttgttga taaatatgct caagcaaacg tcccttcaaa cgtaaatttc
     3781 tttttaggaa cagacggttt gggacgtgat gtattaagcc gcttatttat gggaatgaga
     3841 atctcgttgc tgatcgcatt tattgctgca ttactagatg taacgattgg tgtagcttac
     3901 gggttggttt ctggtttgtt aggcggacgt gtcgataacg tgatgcaacg ttttttggaa
     3961 atcttatcag gaattccaaa cctcgttgtc atgattttga tgttggtcgt ttttgaacca
     4021 ggaatttttt caatcgtggc tgctatggcc atcacaaact ggataccaat ggcacgtatc
     4081 gttcgtgcac aaacaatgaa attaaaagat caagaatatg ttttagctgg attgacatta
     4141 ggtgaatcaa gaatgaaaat tgcattcaaa catattttgc cgaatatctc tagtgtcatt
     4201 attattcaaa tgatgtttag tattccatct gctattttct tcgaagcctt tttaagcttt
     4261 atcggtttag gtcttacgcc tccgtcagct tcattaggaa cgatgttgag cgaagggtac
     4321 aagacattcc tgtatctgcc ttatctatta tggattccgg cagtaacttt atcggttatc
     4381 atgattggct ttaacttact tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa
     4441 gagtgaagac gatgacgaaa attttagaag tgaaagactt agaaatctct tttgatacgt
     4501 atgctggaaa ggtacgagcg attcgcggtg tcaattttca tttgaataaa ggagaaacat
     4561 tggcgatcgt tggggaatct ggaagtggaa aatcagtgac gaccagaagc atcatgcgct
     4621 tgctgtcaag caacgccaat atagatgctg gtcaaatcct ctttaaaggt aacgatatcg
     4681 ttggaaaatc agaaagagag atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc
     4741 aagatccaat gacctcttta gatccgacga tgcccattgg gaagcaagtc gctgagtccc
     4801 ttagaaagca taataaagtt tcaaaaaaag agggacaaaa agctgcatta gaattgctta
     4861 aattagttgg aatacccgaa gctgagaaac gtatcaacag ttatccgcat cagttctcag
     4921 gaggtcagcg tcaacggatc gtgatcgcga ttgcactggt atgttatccg gaaatcctga
     4981 ttgctgatga acctacaaca gcgcttgatg tgactattca agcacagatt ttggagctgc
     5041 tgaaagaaat tcagacaaaa atcgatacat caattatctt tatcactcat gaccttggtg
     5101 tagtagcgaa tgtcgctgat cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg
     5161 gaacatcgga agaaattttc tacaacccgc aacatcccta tacttgggga ctgcttggtt
     5221 caatgccgac attagatagt gcgaacgatc gattgtatgc tattcctggt tctccaccgg
     5281 atttattgaa cccgccaaaa ggagacgcat tctatccaag aaatgaattc gcgatgaaaa
     5341 tcgatgcaga acaagaacca ccattttttg aactatcaaa aacacacaag gcagcaactt
     5401 ggctacttgc tccgcaggca cccaaagtga caccgccaga agaaatccaa cgtcgctggg
     5461 ctatctttaa agagaaacaa aaacatactt acggtcaagg aggaattgct gatgccaaat
     5521 aaactacttg aagttaaagg attgaaacag tatttcaatg taggaaaaaa gaatgaagta
     5581 catgctgtaa atgacatcag ctttcacatt tatgaaggag aaacattcgg tttagttgga
     5641 gaatctggaa gcggaaaatc cactactggc cgtacgatca ttcgcttgaa tgaacctact
     5701 gatggtgaga tattattcga cggtcaagat ataacgaaga tcaaagataa aaaaggtttg
     5761 acgaaattcc ggcatgatgt tcagatgatc tttcaagatc catacgcttc gttgaatcct
     5821 cgtatgaaag tcagagatat cattgccgaa ggaattgatg ttaatggact agctaaatca
     5881 tctgaagagc gtgcaaaaaa agtcgatgat ctattgagaa ctgttggatt gaatccaagc
     5941 cacggtactc gttatcctca tgaattttca ggtgggcaac gccagcgtat tggtattgca
     6001 cgagcattgg cagtaaaacc gcgttttatc atttgtgatg aaccaatttc tgcactagat
     6061 gtgtcgatcc aagcccaagt ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg
     6121 acctatctat ttattgcaca tgatttgtcg atggttaaac atattagtga ccgaatcggt
     6181 gtaatgcata acggcttgtt attagagatg ggaacaagtg atgaaatcta taatcacggt
     6241 gttcatcctt atacagaaag tttgttatct gctattcctc taccagatcc agaccatgag
     6301 agaaaacgca gaagaatcaa atatcaacct gaaccagatg acggacaagt acgtcaactt
     6361 cgtgaaatcg caccagaaca ttttgtctat gcaactgagc aggaagtccc atattacgca
     6421 aaaaaactaa aacgacaaaa ggaatctctt ctggttgcta actaggaggg acatagatgc
     6481 taaaagcttt taaattccgt atgtatccga cagaagaaca aaaacagcaa ttgattcgta
     6541 cttttggatg tgctcgattt acctataacc atttgctgaa aaagcggcag aaaagttggc
     6601 aacaaacagg agtagccaat ttttcgctca cacccgctac attaaaaaaa gaatacccat
     6661 tcttaaaaga agtagacagt cttgctttag caaatgcaca gcttaatttg gatcgggctt
     6721 ttcgcaatta tttcaaggga cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt
     6781 ggcaatctta tacgaccaac aaccaaaccc gtacgatcta tctggaaaat ggctacttaa
     6841 agttaccaaa acaaaaagaa ctaatcaaga taaatcagca tcggccagtt gaaggatcga
     6901 ttcgctccgc tacgatttct gctcgatata atgaagaatt ttatgtcgca ctactttgtg
     6961 atgtttcgcc agtaaaaaaa gaatctttag ctaaatggat cgggatcgcc tatcatccga
     7021 agacattgat ccaaacatct cgacctctag aagtaacttt accaaaattt caccagacag
     7081 aagaaaagct gcaacatgca cagcggaaat tgaatgtgaa agtccgctcc gctcaccata
     7141 gaaagatcag gttagatcag gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc
     7201 tttatctgaa acaaaagaac cagcgagaag attatcttga acaattatct gggaaattgg
     7261 tcaaacaata tgactactta tttgtagaat cttttccaaa agaagaggca catgctgatt
     7321 tttctattca cgactggcat aaattaataa caaaattgcg ttataagtcc caatggtata
     7381 ataaaaaatt tcttttgatc aataccgacg gagcagaaga aagcaattcc gtaagaaaga
     7441 gccaagtagt agaaaaaatg ggcagacatt cggttatcaa gggataaaaa atgaatgaaa
     7501 ctgataggag cgttagggtt agccttgtta ataaaaagtc ttaatgactt ttgtttctag
     7561 gaatttttct tttgaagaaa agctcactaa ttatgacaaa ccaataaaac aatagcagaa
     7621 aaagttcgta cgagcttttt ctgctattgt taatttttta ttcagaaagt ttacgaatac
     7681 atcaaaattc tgaaacaaaa aacgtaactc acaaagagtt gtttttgcgt tttttcttga
     7741 atgattaggt ctgaatcttt tgctcacaac cttattcatg gtgccccggc ttgagctgat
     7801 agatttttga aggacggccg attcctacag gacgttcacc gatctcatcg aaatattgga
     7861 gcatagcttt cttaaaattg gaatgatcga tcgttttata atcgatccct aagaatttag
     7921 caaatacttt tcgagcttcc gtaatcgtga aatctttacc taaaacttgt agaacttgcg
     7981 gctcgtgttc cattttattg actacacggt tgaatgcttt caagatgatt tgactatggt
     8041 caaaagccaa agtatctttg ccagaagact ctccagtgac taaatcaagc gagatagcca
     8101 cttctccgct agataaatgg atcttgttac tatgacgttc tagtgtgaac cagcgtactt
     8161 ccttggcatc atctccggcg attagcggtt cttctccaat aaaggctaag tagctgactg
     8221 tgacgaccca gcctcgcgga tctcgattag gcgtactgaa tgtatgcagt tgctcaatat
     8281 tttcttttga aataaccaca ccagtttctt cctttgtttc tcttaagacg ctttctccag
     8341 ttgactcatc cttttggaca aagccaccag gcaatgccca tgaatttcga taaggatgcc
     8401 cttttctttg gatcaacaaa actttcagct gatcttcttc tttgttatag cacatcagta
     8461 cgatatcgac agtcaaagag ggcttgtcat attctggacg ttcctgtttt tgataccagt
     8521 taagaaattc ttcttcactt gcttcatgtt cataatataa tttttcttct tctttagaag
     8581 caaaacggtt catctgacca cctccattga cagtaaaagt actttttacc ttattgtagc
     8641 agactctgaa aaaaaaccaa ggatttactt gaaaaatcta ggttatcatt taaatatgaa
     8701 aaataaatgg aagtatgaga ttgaataaaa agaaaagcag ataaaacggc aatactttat
     8761 accgctcttc acgaatggca aagaagccac aaataacgat atatacgtca atgataaaac
     8821 gcaaaatcca agctgctgtc ataaaactgt tcaaatgccc atggaggtta aaataaccat
     8881 acatacgaat ataaaaatag ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa
     8941 gtgtaaaaaa aagatgtttc ttctgcatga ttgttccagt cccttactaa gatagatagt
     9001 ctttattata ccacgtgtga ggggagaaat aaaaagccgt tttttagtag atatgaaaca
     9061 aaacaaaaac ccgcatttat tcaacctatt catcctttta aaggatagat tgaacgtaga
     9121 tagaagtcgt ttatccagtc tttttcattt tgaaaaaaag ctaacagcat gcttttttta
     9181 tggtatacta gcaaggaaac gaattcgcat catgcgataa aggaggaaga gccccgtggc
     9241 agaaaaggaa actttttata tcactacgcc aatctattat ccaagcggca aacttcatat
     9301 cggcaactcg tatactacga ttgcatgtga cgcaattgct cgttataaac ggttgatggg
     9361 ctttgatgtg ttttatctca ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc
     9421 agaagaacta ggtgtaaaac cgcaagaata tgtagataaa atggcggctg atgtcaagaa
     9481 attatggaaa acgcttgata tcagttatga taaattcatt cgcacgacag atgattacca
     9541 taaagcagct gttcaaaaaa tatttgatcg tttattagaa cagggagata tttacttagg
     9601 tgaatatgaa ggctggtact ctgtttctga cgaagaattc tttacagaaa cacaacttgc
     9661 agaagtgtac aaagatgacg aaggtaacgt gatcggtggt aaagcgccaa gtggacacga
     9721 agtagaattg gtaaaagaag aatcatactt cttccgcatg agcaaatacg cagaccgtct
     9781 attggaatac tatgaagaac atcccgaatt catccagcca gaatctcgta aaaacgaaat
     9841 gatcaacaac tttatcaaac caggattaga ggatcttgca gtttcacgaa caacttttac
     9901 ttggggagta ccagtcaaga gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt
     9961 atccaattat attactgcac taggatatgg ctccgaagat gaaagtctat tcgaaaaata
    10021 ctggcctgca gatgttcaga tggtcggtaa agaaatcgtt cgctttcata cgatctattg
    10081 gccaatcatg ctgatggccc ttgatttgcc tctacctaaa aaagtatttg ggcatggctg
    10141 gctattgatg aaagacggaa aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat
    10201 gcttgtggaa cgttatggtt tagatgcact ccgttattat ttgcttcgtg ctgttccgtt
    10261 tgggtcagat ggtgtcttta cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc
    10321 aaatgatcta gggaacttat tgaatcgaac gattgctatg atcaacaaat attgtgatgg
    10381 actcgttcct gactatgcct cacttgtcac tccatttgat agtgaactgt ctacgacggc
    10441 tgctaacgtt gtcggacgtt accatgatgc aatggaaaaa atggaattca atacagcatt
    10501 ggctgaaatc tgggtgctga tttctcgtgc aaacaaatac attgatgaaa cagaaccatg
    10561 ggtattagca aaagacgaag aaaagaaagc tgaattgaat agtgtcatga tccatttagc
    10621 tgaatctttg cgtatcgcag cgatattact tcagccaatc atgacagaaa cgccagagaa
    10681 gatcttcaat caactaggat tggactctga aacaatgaat cttgaaggat tacactttgg
    10741 cgaatttcct tcaggaacaa aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga
    10801 tatggaagaa gaagttgctt ttattcaaga aaaaatgtct gaaggtactc aaacaaacga
    10861 agatacagtc aaatgggatc cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat
    10921 caaatttgat gtatttgaaa aagtagaact aaaagtagca gaagtcatca actgtcaaaa
    10981 agtagaaggg gcagataagt tgctgcaatt ccgactagat gctggagaca gccaagaccg
    11041 tcaaatcttg tcaggaatcg ctgaatttta tcctgatcca agcgaattga tcgggaaaaa
    11101 agtcgtcatt gtagcaaatc tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat
    11161 gatcctttct gcagaagcac cagatggctc tttacaagta atcgaagcac caaaatccat
    11221 gccaaatggc tcagaaatcg catagaaatt caaagggggt atgacactag ttgtgtcata
    11281 cccccttttt ccgaaatttg agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc
    11341 aatcatataa tacagaaaca gagaactatc caaaataaat cctagaaaaa gaagtacgag
    11401 cttcgctgat ttttgttaat ttagaataat actaatagga atcgtaaaaa cagaaaaaaa
    11461 gcataatgat aaagggtcgg gcagatttta attgattcat ctggtactct tttttattgt
    11521 ccttacattc ataaagtttg aatgcaatga ctttattaca tctatattta tatctaacag
    11581 tagcgttgat gtgttaatta taagttctat cactttatgt tcaattagtt tgtgctctta
    11641 tatgtgtgat tgaaaatata gataaaaaaa caatcatcgg ctttgacttc gatgattgtt
    11701 ttctttattt tgtaaaaatc ctatcacttg attttatggt aaccttttat ttttgtcttc
    11761 aaacggcttg gtaagagagc agtaagcagt gcaaatggga tcgaaaagac ataccagaaa
    11821 atcgtacgtc cgataccata atgttgatag agctttgtca acatcacgtg taacaagatc
    11881 aaccctaaaa gcaaggagcc agctataaac caatgattat cccaatgcat gacttcacta
    11941 agtgccatca ctccaaaagg atagaaataa aaatagatcc cgactaaggt catccgccag
    12001 ttctttgtaa atgttccgat attcaataaa aagaaaatcg ctgaaagcaa aataccgata
    12061 ggcggaaaaa gcccaaccaa aaaggaatac aacatccccc acaagatcat cacaggacct
    12121 ttgatcaatg caacgattgc gatgaacaaa gtcagcaata ttagtttttg tgataacgtt
    12181 gcaacagtca ttaatccaaa taaaattaat gtgaaccaat gccaatggga ctgctgttgt
    12241 tctttacggg aaaaatcgac ccggttggat aaataatctg ctaatttcgg ataagaacgc
    12301 ttaaaatcca taaactgttc ctccttcgtg gaaaaggtag gtttagtata cgaaaaataa
    12361 aaaagaaata catccgtctt aagagggagt ttttcactga aagtggagaa aaaagaatgg
    12421 taaattaaaa tattctcagt tatactgaaa attataccaa atttcaaaga agaaagagga
    12481 tgggaatgaa aggcaaacta aaacgagaaa tcaatctgtt tggcgcatta gctaccgtta
    12541 tgggaaccgt gatcggtgca ggcgtcttct tcaaaactgc tgctgttact gctagtactc
    12601 agtctacaag tttgacattg ctagcttggc ttttgggtgg tttcttaacg atctgtgctg
    12661 gattgacagt ggctgaatta gctacagcaa tcccggaaac tggtggagct gtaaaatata
    12721 ttgaagctgc gtatggaaaa ttgcccagct ttttattagg atgggcgcaa agcttgattt
    12781 actttcctgc taatattgct gcattatcga ttatctttgc gacacaattg acgaacttat
    12841 tacaattatc taccgattat cttttgttga ttgcagtgat caccgctgtt tctgtgacag
    12901 ggttgaattt gttagggaca aaagtcggtg cttctgtaca atcagtgacg ttgattgtta
    12961 aattaatacc aattgctgtg attgtcatat ggggattatt gaccccagga caaggaactg
    13021 tccagttatt tccaatcgaa gctggaaaag atgttacttt tgtcgaagga ttgagcagtg
    13081 cgttgttagc aacgttgttt gcttatgacg gctggttagg tgtcggtgca atggcagggg
    13141 aaatgaaacg gccggaaaaa gatttgccta aagcaattat tcttggtttg agttttgtaa
    13201 cagtcgttta tctattgatc aattttgttt ttttgaaaac tttgccgatt gatcatcttg
    13261 ccggtaattt gaatgcagct tctgaagcat cagacgtcat ttttggagga atcggtggaa
    13321 agctggtcac tattgggatc ttgatctcgg tatacggtgc attgaacggt tatacactaa
    13381 ctgggatccg cgtgccttat gcgatggctt tagaagacga gctacctttc agtaaacagc
    13441 tgacaaagct ttcaaagaag tttacggttc cttatgttcc tgcggttttc cagttagcag
    13501 tagcttgtat tatgatgagc cttggttctt tcgacttttt aacagacatg ttgatttttg
    13561 tgatgtggct attcagttta ctgattttta tcggtgtctt tattttaaga aaaaaagcgc
    13621 cggaattgcc acgtccatat aaagtaccac tttatccaat cgttccgatt attgcgattc
    13681 tcggtgctat tttcatttta ggtatgacga tgatgacgca aacgaaatta gcattgatcg
    13741 gtataggtgt taccttgata ggtatcccag tctattatca aaaaaagaaa aaacgctcag
    13801 aagagtaaaa tatttcaaca acagacagaa aaagtacgaa ctaggtcctc tagatcttcg
    13861 tgctttttct atttcaataa aacaagttct tctattttga tttttgctac ttctgcaagt
    13921 agtgcttatg tgctaaagta agaatgaaaa ggagagataa acatgatatt tgattctcat
    13981 acccatttaa atgctgaaca gttcaatgaa gatattccag aaacgattca gcgagcacaa
    14041 gaaatggggg taacgaaaat ggcggtcgtg ggcttcgata cgccaacgat tgaaaagtct
    14101 ttgcaattaa gccatgacta tccaaatata tacagtatta tcggctggca tccgacagag
    14161 gctggcagtt atacgaaaga aatcgaaaat aaattacaag aacaattgac tttaccaaag
    14221 gttgtagcat taggggaaat tggcttggat tatcattgga tggaagaccc gaaagaagtc
    14281 caagataaag tatttcgcag acagattgct atcgcaaaag aaatgaacct accaatcagt
    14341 attcatacta gagaagcact tgaagacacg tatcaaatcc taaaagaaga agatgttcga
    14401 gatatcggtg gaatcatgca tagtttcagt ggtgattatg aatgggcaaa acgatttctt
    14461 gacttaggga tgcatatttc atttagcggt gtcgtcactt tcaaaaaagc attagacgtc
    14521 caagaggctg cagcaaatgt accaatcgaa cgattacttg tggaaacaga tgccccttat
    14581 ctggcaccag ttccttttag aggaaagcga aatgaaccag gctatactcg ttatacagta
    14641 gaaaagattg ctgaacttcg gaatcttccg acagaagaag tggctgccca aacatggaca
    14701 aatgcgcacc gattatttgg tctaagcgaa catggataag atgaagatcc aagagattat
    14761 agtcgttgaa ggaaaagacg atacaagaag gctgcgggaa gtagttgatg cagatacgat
    14821 agaaacaata gggtctgcaa tcaatgaaga gatcctgatg caaattgaac atgcccaaga
    14881 gactcgtgga gtgatcgttt ttaccgatcc tgatttttca ggagaaaaaa taagaaaaat
    14941 cattatggaa gctgttcctc aggcaaaaca tgcttttttg ccgcgcacac aagctaaacc
    15001 aaagaaaaag ggaagccttg gtgtcgagca tgcaagtgat gctgctattt tagaagcatt
    15061 gaaaaaggtc gtgacgccag acatttccca aacggctgtt tctgaaatca ctagacaaga
    15121 ccttttagac tatggtttaa tagctggggc acaagcaaaa gaaaaacgag aaaaactagg
    15181 tgacgagttg cgcatcggtt acacgaacgg caaacaattg gaaaaacgtt tgaagatgtt
    15241 tcgtattacg ccgaaagaac tcgcccaagc aatgaaaata gtggaggaac aacatgagtg
    15301 aatatcgtga aattgcgaca ccctctagaa caaaagagat tttattgaaa catggttttt
    15361 catttaaaaa aagtttagga caaaattttc tgactgaacc aaatatttta aggaaaatcg
    15421 tagaaactgc gcaaatagat gaacatacga acgtgatcga agttggacca ggaatcggtg
    15481 cattgacgga acaattagcc aagcatgcaa aacaagtcgt agcatttgag atcgatgatc
    15541 gtttgatccc tgtattggct gatacgatgc aaccctatga caatgtgact attgtacatc
    15601 aagatatttt aaagaccgat ttgtcaacag ctgttagaga aacatttcat gaggagcttc
    15661 cgttaaaagt cgtagcgaat ttaccttact atattacgac accgatcatg atgcattttc
    15721 ttgaatcaga tttggttgtg gatgaactag tcgtcatgat gcaaaaagaa gtagctgatc
    15781 gtatctctgc tgaaccagga acgaaagctt atggttcatt atcgattgct gttcaatatt
    15841 atatggaagc cagtttagca ttcatcgtac caaaaacagt atttgtccct cagccaaatg
    15901 tagattcagc gattttgaaa ctgacaagaa gagatatacc ggcagtcgaa gtgacagacg
    15961 aaaaagcatt tttccgattg acaaaagcgg catttcagca aagaagaaaa acgctttgga
    16021 ataacttgca gcattcgtat ggcaaagatg accagacaaa agcatggtta gcaaaaagct
    16081 tggaaacagc tggaatcgat ccaaaacgtc gtggggagac tttgtcatta caagaattcg
    16141 ccgcattaag caatgcgatg tcagaaaata tgcagtaaaa aaagaggttg tgacagaagt
    16201 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcatg
    16261 tttttcgttt gtttgggctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    16321 aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa aacagcttct
    16381 catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac tattatgccg
    16441 aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt gatcaaaaaa
    16501 ttcttatact tgatactcac actcattttg tatggattta gaaaagaata tgtgtcttga
    16561 cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca tattaggaga
    16621 gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta ttgattggga
    16681 tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct tcctttactt
    16741 tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt gcagtggctg
    16801 ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc gcattgcgag
    16861 cattgtcaca tcttgtgttt gcgttgattg gggcttggta cttgcaaaag catccgagta
    16921 tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg ttcaattttg
    16981 tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt tactttattg
    17041 gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc atcttgatgg
    17101 ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt atagcaggaa
    17161 cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa aatcggaaag
    17221 agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc aaaaaaaaaa
    17281 ctctcatcta gtaatggata ataaatcctc taagatgaga gttttttgtc ctaaaaagag
    17341 ctgcaccaac atcttctggc acagctctaa cagggggcaa tttatggaag tttgatgact
    17401 tgttctgttt ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt
    17461 tcgcctggtt tatggaaatc tcttgcatct acttttgaga tgaactcagt agaaccagtt
    17521 tttgtgtaaa gcatcgtttc agctccaagt aattcggaaa cgacaacttc tgatttcact
    17581 acggcttcag gtgaagcttc aagggctact tgttcactgt ggatgtcttc tggacgaata
    17641 ccgaagataa cttcttttcc ttcataacct ttttcaacta atactttgtt ttttccttca
    17701 ggaatacgaa gcttaagccc atgtccatct gtgataacac catttttcaa tgtcacatta
    17761 aagaagttca tggcaggaga tccaataaag ccggcaacga acatattgac gggtgtatca
    17821 taaacttctt tcggtgtacc gatttgttgg ataaatccgt ctttcatgat aacgattcgg
    17881 tcagccatag tcattgcttc tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga
    17941 cgctggtgaa gtttggcaat ctcagcacgc atggctacac gtaatttagc atctaaattt
    18001 gataatggct catccattaa gaagactttt gcatcccgaa cgattgcccg tcccaatgcc
    18061 acacgttggc gttgtccacc agataaagca gctggttttc gtttcaagta ttctgtcaga
    18121 cctaagatct ctgctgcatt gtccacacgt tttttgatgt ctgctttatc atatttacgt
    18181 aatttcaagc caaaagccat attatcaaaa acagtcatat gaggatacaa tgcgtagttt
    18241 tggaaaacca tggcaatatc acggtcttta ggtgccacgt cattcatgac ttttccgccg
    18301 atatttagtt ctccttcaga gatatcttcc agtcccgcga tcattcgcaa agtagttgac
    18361 tttccacaac cggaaggacc aacaaaaacg ataaattcac ggtctttgat tgatagatta
    18421 aaatcagtta cagaataatt ttctgcattg tcatattttt tatatacgtt tttcagagcc
    18481 atttctacca taatacgttc cacactttct tttttagttt tattaactat ttaaagtata
    18541 aatgaaagcg ttaactaact tcaatgtcat gttgaacaag aattttttct tttttttgtc
    18601 aatgtgctag ttttaagctt taataaatat ccataagaaa taaaaactga tacactggag
    18661 taaataagta tgttaacaat atcaaaagga gtgtctctta tgaaaatcgc tttgatcgct
    18721 catgaccgaa aaaaacctat gatggtgaaa ttagcaacag cgtataaaac aatattggaa
    18781 aaacatgaac tttttgctac cgggacgact gggcagagga tcacggaagc aaccggcttg
    18841 ccgatccatc ggtttaaatc cggaccgtta ggcggggatc agcaaattgg agccatgatt
    18901 tcggaggatc agttagattt ggtgattttt cttcgagacc cgctggctgc acagccccat
    18961 gaaccagatg taacagcact tatccgttta tctgatgtct atgagattcc gttagctacg
    19021 aatatcggaa cagctgaggt gctactaaga ggactggaag ccggcttcct agattttcga
    19081 agaatcgtcc atgaaataga caagcatcca ctttcatttt gatggataag cgtatggaaa
    19141 aataacgttt aatgttcgcc tatatgtagc tataaaagct aaacaaactg ataatatgcg
    19201 atataatacg aacgtattgg ataatataaa caataatatt agtttttcgt atttgcagta
    19261 aatgtgaaaa tatgctatag tttctgtggg aaaagatccc aaaaataata cagagtagaa
    19321 aataaagcgt taagtgctaa gaggatggga tgttgcctct tggacgaagg acgtaatgtt
    19381 cgcggtttta ttttcgcatt cgctgcatga tacgatgtag cagctcttaa aggagatgct
    19441 aaaaatgaag aaacaccggt tagacgcacg tatgattgct attatgggct tattgattgc
    19501 attgatggtc actttgtccc gactggtagc aatcgagacc ccatttatta aaatcagtgt
    19561 cacatttatt ccacaagtga tcatgggcat tctttttggt cctttttggt cagggatagg
    19621 cgcagtttta gctgatttag taggaatggc cctcttctcg aaatctgcat tctttattgg
    19681 atttacattg aatgcattta ttgaaggagc aatctacgga ttctttttct atcgaaaaga
    19741 gatcacttgg aaaaatgcca ttctggctac gcttagtgtc acgctgatca tcaacttatt
    19801 tttgacacca ttatggcttg cgttgatgta ccatgtgcca ttgttcagct gggtcgtttg
    19861 ggcgccccgc ctgttaaaaa cagtaatatg gttaccgatt caatcaattg ccatttatta
    19921 cgtaggacgt agtattcctt acaaaaaaat tttaagaagt ttagcgatcc acgctaaata
    19981 aaaaacaagc agtcttccca tcccagaaga ctgcttgttt ttattgtgct gtgaattgtg
    20041 cttgatagag ccgtcggtaa taaccatctt tttgaagcag ctcatcatgg gtgcccattt
    20101 cgacaatagc tccctgatcc atcactaata tctggtccgc atttttgatt gtggacaggc
    20161 gatgggcaat cacaaaactg gtttttcctt ccatcattgc taagaaggct tgttggattt
    20221 ttttctctgt caatgtatcg actgagctgg tagcttcatc taagatcaac atagggggat
    20281 tactgatcat cgttcgagca atcgtcaaca gttggcgttg tccatctgat atctttaacc
    20341 cttgaccgcc aatcttagta tctaattttt ccggcaaacg ttctacaaat tcatacatat
    20401 aagaggcttt tagggcatca ttgatttctg cgtcacttgc ttccggattc ccaaaacgaa
    20461 gattttctcg caatgtactg tcaaatagcc aagtatcttg taacaccatt ccaaaacttt
    20521 tacgtaatgt atcacgttgg atagagcgga tgtcatggtc atcgactgta attgttcctt
    20581 ttgttgtgtc ataaaaacgc atcagcaaat taacaagtgt ggattttccg gcccctgttt
    20641 ttcctactat agcaatggtt tgacctggat aagcagtaaa attgaaatct tcgatcaaac
    20701 gctgcttggg gtcataagaa aaagaaacat gatcaaaaga gacttttcct ttgatatttt
    20761 tgggttccaa ggcataggca tctggcaaat caggcttttc ttctggctga tcgatcaaat
    20821 caaatgctcg ttctagacca gaaaaagcgg tttgcagttg tgtcgtgatt ccagaaagtt
    20881 cgataaatgg tttagaaaac tgacttgagt agatcgtaaa gctggaaatc acaccaacag
    20941 tgattgacga accactcttc aatgccaata ggccgccaac taaaccaatc gataaataag
    21001 caaggtgatc tacaaaacga gaagacgggt ttgtcaagga agaagaaaat tgtgcccgtt
    21061 gtcctttttc atatagatca gcatttagca gttcaaattg ctgttgacta gcttcttctt
    21121 gctgaaaagc tttcacgatt ttctggttac cgattcgttc ggtcacaaag cccgagatat
    21181 ccccaataat cctttgctgt ctatcgaaat ttgtttgtga agcacgagca acgatccaac
    21241 tcacaaggaa aataattggt gtagaacaaa gaacgaccaa tgtcagaagt ggacttagac
    21301 gcatcataaa aataaacgaa agaatgatga ttgccactcc agaaaataat tgattgaagg
    21361 ccgcagaaat tgcaatggaa atattgtcca tatcatttgt aaatcgactg acgatatttc
    21421 cgtgggagtt ttgatcataa taacgaagtg gcaatcgatt cagatgagca aatgcatctt
    21481 ttcttaattt tgcaacggat aagtaagcta accgattgct taacacttgg atgagccatt
    21541 gactgactac gttgattaaa acgataccac cgaatagaat aagtaaatgg aacagtcgat
    21601 tgaagcggac ctgtccttct ccaatcattt gatcgatact ttgaccaatt tgtaacgtca
    21661 tcacaactgt tgcaatccca ttgatgattc ctagtatcac agctagccaa atgtcttttg
    21721 cataggcagt aaggtaagga atgaatcggc ggagcgtctg caaggataat ttttttactt
    21781 tcataactat tcttcctcct gtgatgcgac gatttcctga tattctgctg attgagcaag
    21841 taattcttca tgggtaccct gagcagcaat cttaccttga tccatcacta agatatgatg
    21901 tgcttcttga atagagcgga cacgttggga gataataatc actgtactgc caagataagt
    21961 ctgtagcgcc ttacgcaaat ccaagtctgt ttgataatcc aacgcactta aagagtcatc
    22021 taaaatcagt aatgctggct tggcaatcaa agctcttgca atcgtcagtc gctgtttttg
    22081 tccgccagag aaatttttcc cattttccat gacttttgta tctaaaccat ttggaagctg
    22141 acggacaaat tcttctgcct gagcgatttt cagagcttcc caacattctt tttctgtggc
    22201 gtctttctta ccccattgaa ggttttcacg aatagtcccg gtaaatagta cagaagtttg
    22261 gggaacaaga gcaatttgct gacggagatg atccagctgc caagaacgaa catccattcc
    22321 gtccattata actgtaccgg aactgacatc atagaaacgc ggaatcaatt gtgtcaaagt
    22381 cgttttcccg cttccggttg gaccaatgat tccaaggact gtatttcgct tcagctgaaa
    22441 tgtaatattt tggagagaaa gaccagaatc aggagtataa cggaagtcaa catggtcaaa
    22501 agcgataatt gcttcagaag aaaaatcggg agtagtactg acctcagctt caagaatcga
    22561 attttctgtg tctagcacct ctttcacccg atttcctgaa gcttcggcac gtgtgaagat
    22621 cacgacgagg tttgaaacaa cgatcagtgc caaaagcatt tgattcatat aattgatcaa
    22681 agcaagaact tgtccttgtt gcaagctacc aatatttact ttgatcccgc caactgtcag
    22741 caaacagatg atcccgacat tcatgatcaa ggttgtagca ggtgataata gggcagagat
    22801 attggaaaca cgtaaataat tgttcgctag atcatttgtg cttttatcga acttctctgt
    22861 ttcttttttt gtctgcgcaa atgcgcggat gacacgaaca ccgctaagat tctgactaac
    22921 gtgttcattg agctgatcta gtttttcttg cactttttgg tagagaggaa cggttttttt
    22981 gatgataaaa aataaaatga ttgagaaaat aggcaataat gctaagaaaa acagacctac
    23041 ttcccagtcg atcacaaaag ccatgaccac tgacccgata cttaaaaacg gtgctctgat
    23101 caataaacga atgaccattg ctaaagctag ctgaagctga ttgatgtcgt ttgtcattcg
    23161 ggtgatcaag gtatctgttc caaaatgatt caactcgttg tgagagaaag tattgatttt
    23221 tttgattagc tgattacgta actctgtacc aaatccttgt gaagctactg aagcatagta
    23281 ttggcaaatg atagcacaaa tcagaccaat cacagacata gcgaccatcc ataagcccat
    23341 ttcaatgatt tttcctcggt cattcatttt tagtccatta tcgatcaagg aagccatcat
    23401 taaaggcagg acaagttcga agacagcttc aagaaatttg aagaacgggc ccaaaatgat
    23461 ttgcttacga tagtcttttg cataacgtaa taactgaatc atatgattcc tcctattatt
    23521 tatttgtcca caattgaaaa aacaagtact accacattgt agcttaggaa atttagaatg
    23581 aaaagagaac aagccttttc ataaatgaaa gttttattat gaagaagctt tagaaattga
    23641 atagactatt ttttttaaaa aaaagaaaat gagaaaacat gccagtctct tcattcatag
    23701 gaacgtctat tttacttaat ttttctataa atgaattctg cataaaaaat atatcaagta
    23761 aatgtttata gatgaaactc tatttacttc ttttttaatt tgttcttgca atcttttttg
    23821 ctacgtaatt tttgtaaaaa agtgggttga gagaaatcta attgattttc aaagaaggag
    23881 tatgaaaatg agaaaaatat ttattcgttt attgtgggta ctatcttaga ggaataaata
    23941 atagtggaga aaactatggt ttggcgcaga ttctatcagg tttgatggaa cgaagacaac
    24001 ctcccgacat caagtcaaaa gctacttgag attttgatct gaaaataaaa aaagaaaaca
    24061 aggtaggaac atcaatgttt ctaccttgtt ttcgtaatca atgggaaaaa actaagagtg
    24121 gtgtacgtaa tcaataaaaa ggatggtgac aacagtacct ctttactctc tgaaagcatc
    24181 ataactcgtt tttcctcatt atgccaataa attgttgtta taccacatat aaggatttgt
    24241 aatagctaaa aacagacagg aaagttgaaa tctgtgaatc ttattttgta tggaaggatt
    24301 tatagaacgc aaaaatcaag ataaagatga ataaaaatcc tgtttcggct atatattgcg
    24361 tatctacgcc aataaaacga gtttgttctg tca
//
LOCUS       DACDEG010000032        23700 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.032, whole genome shotgun sequence.
ACCESSION   DACDEG010000032 DACDEG010000000
VERSION     DACDEG010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.032"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(461..1090)
                     /gene="udk"
                     /locus_tag="IV142_001353"
     CDS             complement(461..1090)
                     /gene="udk"
                     /locus_tag="IV142_001353"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP8485022.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1174..1869)
                     /locus_tag="IV142_001354"
     CDS             complement(1174..1869)
                     /locus_tag="IV142_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP8485023.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1882..2598)
                     /locus_tag="IV142_001355"
     CDS             complement(1882..2598)
                     /locus_tag="IV142_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP8485024.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2928..3536
                     /locus_tag="IV142_001356"
     CDS             2928..3536
                     /locus_tag="IV142_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP8485025.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3861..4184
                     /locus_tag="IV142_001357"
     CDS             3861..4184
                     /locus_tag="IV142_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP8485026.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4366..5736
                     /locus_tag="IV142_001358"
     CDS             4366..5736
                     /locus_tag="IV142_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP8485027.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5866..7167)
                     /locus_tag="IV142_001359"
     CDS             complement(5866..7167)
                     /locus_tag="IV142_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP8485028.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7521..9512)
                     /locus_tag="IV142_001360"
     CDS             complement(7521..9512)
                     /locus_tag="IV142_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP8485029.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9656..9922)
                     /locus_tag="IV142_001361"
     CDS             complement(9656..9922)
                     /locus_tag="IV142_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8485030.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="IV142_001362"
     CDS             complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="IV142_001362"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP8485031.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="IV142_001363"
     CDS             complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="IV142_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP8485032.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13266..13829
                     /locus_tag="IV142_001364"
     CDS             13266..13829
                     /locus_tag="IV142_001364"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP8485033.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14082..14603)
                     /locus_tag="IV142_001365"
     CDS             complement(14082..14603)
                     /locus_tag="IV142_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP8485034.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14606..15448)
                     /locus_tag="IV142_001366"
     CDS             complement(14606..15448)
                     /locus_tag="IV142_001366"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP8485035.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15441..16166)
                     /locus_tag="IV142_001367"
     CDS             complement(15441..16166)
                     /locus_tag="IV142_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP8485036.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16342..16638
                     /locus_tag="IV142_001368"
     CDS             16342..16638
                     /locus_tag="IV142_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485037.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17206..18798
                     /locus_tag="IV142_001369"
     CDS             17206..18798
                     /locus_tag="IV142_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8485038.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18811..19551
                     /locus_tag="IV142_001370"
     CDS             18811..19551
                     /locus_tag="IV142_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8485039.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19719..21068
                     /locus_tag="IV142_001371"
     CDS             19719..21068
                     /locus_tag="IV142_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8485040.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21196..21786)
                     /locus_tag="IV142_001372"
     CDS             complement(21196..21786)
                     /locus_tag="IV142_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP8485041.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21933..23285
                     /gene="brnQ"
                     /locus_tag="IV142_001373"
     CDS             21933..23285
                     /gene="brnQ"
                     /locus_tag="IV142_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP8485042.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6794 a         4772 c         4511 g         7623 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttcaaacgg tcgcagtccg ttcaactgtt tcgctgcgca ctatttatgg tttaagaaac
      481 tagctacttt tgttgtaatc aaatcaatag caacgtggtt ttctccgcct tctggaacaa
      541 tgatatccgc atagcgcttc gttggttcga taaattgatg atacatcggt ttaacaactg
      601 tcaaatattg ttcgatcacg gagtccaatg tgcgtccccg ttcttccata tcccgtttga
      661 tacgacgaat gatacgaatg tcatcatctg tatctacgta tactttgata tccatcaagt
      721 cacgcagacg ttcgtcttct aaaatcagga ttccttctag aatgatcact tcttttggtt
      781 cctgaatgat cgttgcctga cttcttgtat gcgctacata gtcatacacg ggtttttcaa
      841 tggctttata gttcagtaag tcgcctacat gctgaatcag cagatcatta tcaaaggcaa
      901 atgggtgatc gtaatttgtg ttcaatcgtt cttcaaaact caaatggctc tgatccttgt
      961 aataagaatc ttgttcaagc atcatgatgg aatggtccgg gaagttgttg aaaatcgcac
     1021 ggctgacact tgtctttccg cttccggatc cacccgtcac accaatgata attggcttgc
     1081 tttttgtcat tgactaaaac ctctttcgta tccgtttttt cattctaatc tattatagcg
     1141 aaaaaacacg aaaatgctat atttttctga aaattaatcc ttttcaattt ccaataatga
     1201 ataaagttcc tctaatgaat cgattcgata agtaggctgg atttcttcga tttctggtaa
     1261 tttatttcta ttgaaccaaa tcgtatcgat ccctgccata tttcctcctt tgatatcaga
     1321 agtcaaagaa tcaccaatga tcacagtttg ctttttatca aaattaggaa tgtgggaaaa
     1381 gacatgatca aaaaattctt tcatcggctt ttgcgctccg acagcttctg agacaaagat
     1441 atccttgaag taatcaagca ttcctgcatc tgttaatcgc cgatgttgcg tgtgcgacac
     1501 accgttcgtc acaatataaa gatcagcatg cggatggacc tttttcagga tttctagact
     1561 tcctggaaca agatcatgac cttggttcaa gtagtggcgg tagcgttttt ccatttgatc
     1621 actatctact gttttcccaa attgctggaa aagtaaccca aaacgactgc ctattatttc
     1681 ttctcgtgat agtctgcctt tttcaaactc ctgccataaa tgatgattca aagccttata
     1741 gctggtttcg atttctggtg tcaatgtgat tccttgttct gcaaacaaag cacttaaagc
     1801 attggcttca gttaattgaa aatctaacaa tgtatcatct acatcaaaca ataaagtttt
     1861 atatttcatc tttttgacct cctatttaaa ataaatactt aactttttca tctctgctaa
     1921 tgtcaacgat cgatactcgc ctactcctag ctgttcatct aattccaatg gtcccatgct
     1981 tagccgtttc aatgcggtca ctttctttcc ttgggctaag aacatctttt tgacttgatg
     2041 gaattttcct tcttggatcg tcaataaaac cgtactttgg tgcttgtctg ctttgagtat
     2101 ttttaaatca gccggcttac attgataccc atcagcgaat acgattccat tttcaaatgc
     2161 tgttacgtct gccggtgtca ccctttcgtt cacaactact tgataagtct ttgaaacctt
     2221 tttttctgga agtagcattt catatcccaa ttgtccattc gaagtgatca gcaatagtcc
     2281 tgttgtatct ctatctagtc ttcccactgg atataatggt tggaaacgat cggcacttgc
     2341 aatcagatcg attactgtcg tcttttctgg atctgataca gctgtaacag ctttttcagg
     2401 tttattgaga agataatagg actcatttgt ttctaagtgt tcacctgcta cttcgatttg
     2461 atgcagtaaa ctatctacat ttcgattttg tcgaaattcg actgtgccat ctatcatcac
     2521 ttgcttcaaa gcaaacagcc gcttcatttc cttgcgtgtc gtttctaatt tttcttctat
     2581 taatttatct aaacgcatgc tgtttccttt cttattacta ctaattaatt ataccttgaa
     2641 tattcttgct tgcaaataca tcatcatagt tattccttga cttaattaac agttcacgtt
     2701 acggttaaat agtaaagtga attggatttc tcatacacag cgtaatgacc tggtggagaa
     2761 aagttgaatc ctgactttct aaggggagat gcgcgcatct ctccttttgc gcttttttaa
     2821 gcgccctgcc ttcgatgatg tgcacgagat tagcctttag aattcactta aataaaatct
     2881 gtccgttacc aaagcagaca gtcacatttt aaggaggaat tttggttatg tcagtatcat
     2941 tagaagtaag taaaagagaa gtacgtcccc gttcattaag aaacaaactt cgtcacgaag
     3001 ggaaagttcc agctgttgtt tatgggtata aagtagaaag tacccctatc tcattcgatg
     3061 gtttgacact aagcaaattg ttgcgtgaac atggagcgaa cacagtcatc acgatggaca
     3121 tcgatggaca aaaagtcaat acattggttc acaagatcca aacgaacact tttaccaatc
     3181 atttcgaaca tgcagaattt ctatctgtaa acatgtcgga agaaacagaa gtcgaaacag
     3241 aagtcgttct tgttggcgaa tcagtgggtg ctaaatctgg tggagtgtta gctcaaaact
     3301 tgtatacagt agtcgtatct gctacaccag acaaattgcc tgaacgaatt gaagttgacg
     3361 tgacaaactt ggaattaggt gattctatta cagtagccga tcttccaaaa aacgacgatt
     3421 acaaattcgt gaccgacagt gaagagcaaa tcgctgctgt agtcgctcaa agtgaagaag
     3481 aagaaagcac aggtgaaggt ggcgaaccag aagtgatcgg ttcaacagaa gaataatact
     3541 gaaacaatca ggtggtgtta gaagcggcgg gtcagcagct tttaacacgc cttttttatt
     3601 tctaatcgtt tattttcata tggggcttcc acttttgtaa atggctttac aaacgaaaaa
     3661 tcctaattta ccaagctttt cctcgcatac ttttatcgta cctttataaa aatttatact
     3721 ttgaaaaaaa gtattcaatt attgttgcat ttttcctgat tctttattag aatgaaccct
     3781 cgttgaagta agtatttcta gtttatccta ggattaatta taaaaataaa acattcgttg
     3841 attcgaagga ggttaaacag atgtttgaaa gttttgatag cagccgcaac cgttacgctt
     3901 cagttggtgt cgtatcaagt ttgccagatg aattgattga cagtatatgg tttattattg
     3961 acctagatct taagggagtc attccattag aaaatatatt aacctttgat ttgatcgaaa
     4021 atcgtggaaa agtgacgatg catttttctc aagcaggaag caatgtggaa atggatattg
     4081 atttgccgtt tgcctattcg aaccaatttc ctgctcaagt atacgcctat gatgacggta
     4141 cgagagaaac gatcctttta ccaagtgaga ttagacaata ttaaggtgat agataaacgt
     4201 ggatgtccct tattttacag accaggccaa gttgttgatt ctttgactca attcttggct
     4261 tctttttatg gagtttcata aattatcatt gatttctaat tggtattctc aggataaacg
     4321 ctatatacta ctaactaaat tttaaagata taaggagttt aaaagatgcg cgatttaaca
     4381 aaaggaagcc ccgctaaact gattttgatg ttcactgttc ctttactgat cggaaatgtt
     4441 tttcagcagt tctacaatat gatcgatatg atcatcgtgg gtcagacgct aggtaaaaat
     4501 gcgctggcag cggttggtgc aactggaagc ctgacatttt tgatcattgg ctttgcccaa
     4561 ggtttaacag ctggtttagc cattattacc gcacaacgtt acggtgcaaa agattatcgg
     4621 ggtctgaaga aaagttttgc tgctagtgtt gtcattagtc ttgtcgtcac tgtgatttta
     4681 actgtcctaa gtctcctgtt tattcgtccg atgcttcaat tgatgcaaac tcctcctgaa
     4741 atacttgatc aagcacaaac atttatttca attattttat tagggatctt tgcatcagtc
     4801 agtttcaatt tgctttctaa tgtcattcgc gcacttggtg acagccgcac accactattc
     4861 tttctgatca ttgcggtcat catcaacgtc gtacttgatt tgatctttat tatctatttt
     4921 ggcatgggtg tggaaggtgc agctattgct acagtaatcg cacaagtttc ttccagcgta
     4981 ctgtgtctag tgtatatcaa gaaaaaaatg cctttgttac agttacgcaa aaaagatttc
     5041 aaatttgaaa aagaagagtt tgctgtacac ctaaatgctg ctttgccaat ggcgtttcaa
     5101 tcttcgatca tcgctgttgg tgctatcgtg ttacaagcag cactaaatag tctagggaca
     5161 gatgtcgtag ctgctcaagc cgctgctggt cgtatcgacc aatttgcaaa tcagccgatg
     5221 atgtcatttg gtattgcaat ggcaactttc actgcacaaa actatggtgc gaaagaatat
     5281 ggccgaatct tgcaaggggt acgtcaaact ttactgatga gtgtcggatt tagtctctta
     5341 gcaggtgcta tcgtcatttt cttcggtcat ttctttgttc aattgtttgt tagcccatca
     5401 gaaacacgtg tatttgaatt agcacaaacc tacttcaata tcaatgggtc tctttactgg
     5461 atcttagcag tcctgttcat tttacgctac acacttcaag ggcttggaca aagcaagatc
     5521 cctacgatcg ccggtatgat ggaattgctt atgcgttctt ttgcagctat cgttcttaca
     5581 ggaatgtggg gatacccagg agcagcagca gcttctcctt tagcttggat cggttctgtt
     5641 gccgtgttgc tttactctta tatgcgttcg atgaaacaat tgaaacacat gggtgaagag
     5701 gaacatttca cattagaaca tgcagaagca agatagcctt gattaaaaga aatcgttgcg
     5761 gctaaaaatg aaaactttta ttgaaaatat cttcttcgtt taccgaaaac aggtttttta
     5821 gataacaaaa aggctgtgac aaaaaatgtc acagcctttt ttcttttatt ctgtttttct
     5881 ttccgtggac tctactttga aataatcgat atgtgcatgc tggtattcta ataccgtgaa
     5941 acggaatgat gcaatctcaa tgattgttcc tgcttttatt tctttatatc ggctagaagc
     6001 aaagcccgca agtgtagtaa aattcgtttc ttcaaactct ggaatctcta catgaaaata
     6061 acgctggaaa tcatctaatg ttagtttgcc ggagatgata taagagccat ctgactgttc
     6121 cgctatcaat gcttcgtcca aatctgtctc atctcggatt tccccgacga tttcttctaa
     6181 aatatcttca atcgtcacta agccgcttgt ccctccgtat tcatctttta agatagcaat
     6241 atgcttaccc ttttttcgca tgatcgtcaa taattttttt atagggatcg tttcagttgt
     6301 tacgatcggc tcttcaatca actgttctac tgtcagctgg tcatctttat aagattgctt
     6361 gatcaaatct ggcaaggtga cataacctaa aacattgtct tttgattcta agataaccgg
     6421 atagcgagta tgtccttgtt caatcgaagc ttttatcgct tctttgacgg tttctgaagc
     6481 ttcgaatact tgcatggacg tacggtctac ttgtatatct tttgccaagg tatcatcaaa
     6541 ttcaaacacg ttttccaaat aataatattc ttctttggtc aactctcctt tttcataaga
     6601 attcaatgcg actaatgtca gctcttcttg agacaatgtc tcttctcctt cagatacgag
     6661 acgcacacct aatagcttcc ctagccctgc agctgaatga ttcaataccc aaataaaagg
     6721 aaacattact ttataaaaat aatgaagtgg tttaactacg aacaagacga ccttctccgt
     6781 attgacgata ctatagctct taggaagcag ttcgccaata accacatcga tatacgtaac
     6841 taaaataaat cccagcgcca cagagattgt actgctaata gccgaactaa aaggtagttt
     6901 accgatcact ggttctagta aagccgcgac agatccttca ccgacccacc cgatgatcaa
     6961 accggttagc gtaattccta attgactagc tgataaatag ttatctaaat ggtgtgtaac
     7021 atgaagagct agcttggcgt tctttacatt ttccttttct agttgttcca atcttgatcg
     7081 tctgattttc actaaagcaa attcacttgc tacaaaaaat gaagtaattg caatcatcaa
     7141 aaataaaata atgaaattga taaccatatt ctcccgcctt tctctttttc tattgtaccc
     7201 tttttctaca aaaagattga aaataatgcg tattcatatt taggaataaa aaaggagatg
     7261 cggcattttt cagccacatc tccttatcac ttcaaaatca ttgcgttact tgttgctaaa
     7321 cagctttttc atgacctctg ataaaaacgg tattcaaaag cttgcatcta cggcattaag
     7381 cccaaacaga tgaaaaacat gagaagctgt ttttatctgc tcgttcttac tgcttgttgc
     7441 agtacagctt tttcatgacc tctgataaac ggtgttcaag tagctgacat tcgacattag
     7501 ttcaggtcaa agcgctactt tcacaaccgc tctttcaacc atgttttccg agtatttcca
     7561 atgataagcg gaacagtttc atgttttact tcgctgtact ctagcccaaa ccatgtttct
     7621 ggcgaggtag cgattttttt gattgccagt tttgcctgca tgatttgacg ttgccatttt
     7681 cctagagttg tcacattgga aactacttca tcaatcagga caaattggaa atcgccaact
     7741 tctcgccctg gtgtcagaga gtatctttgt ggctgttttg gcaaacggcc ttgtttcatc
     7801 aaatcatgga tgacttggcg aacatatacg ttgatttcct gttggaccct gaaacctaag
     7861 aacagattca cttggacaat aaaatcagtg tccatcatat ctacttgata ttctttcgta
     7921 tatggctcat ccgtcacttc cacatgaaca aaccaataga ctcgtgctct tttaggccgt
     7981 ttatctaaaa tcgaatagat gaactgtcgg ccaactttat cttcagccat atcaggaacc
     8041 aagaagacca catttgtctg atagattggt aaagaagtat catcacgtaa ttctctaagc
     8101 tgcgggatat aatctctcaa agacacatcc tcggttgacg attcgcgaat aatgttccct
     8161 tgttcccaaa taaacatcac gactaagatg atcaaaccaa tcagaacggc tacaaaaccg
     8221 cctcggaaga atttgacaac acttgagata aagaaaacga cttctatcgc accgaagaaa
     8281 aaggtaacta aatatgcaag ccaacgagaa tgcccatttt ggataagata aaagtaaagc
     8341 agtgctgttg tcatcaacat cgtcacagtg atcgatagac cgtaagcagc ttccatatga
     8401 tgagacgttc ggaatgtgac aaccactaga gaacaggcga tccataaaat cagattgact
     8461 gctgggatat acatttgccc gatactgttc ccaggataca tgatctttaa ccgaggcagc
     8521 agtttcaact tgattgcttc tgaaactagc gtaaacgaac ctgtaatcaa tgcttgcgaa
     8581 gcaatgactg cagcgactgt tgcaaaagca acgccgatca cgatccaact ttgaggcatc
     8641 atttggaaga atggattcag gttcttgatt tgctgtgctt cagggttttg ataaacattc
     8701 aacaaccacg cagcctgccc aaaataattg ataaccaaac aaattttgac gtaaggccag
     8761 ctcagacgaa tatttcgttt gccgacatgt ccaagatcgg aataaagcgc ctctgcacct
     8821 gtcgtagcca ggaaaatatt tccaagaatg aacaagccta atttgttatc tgcactaaaa
     8881 agcagctgga tagcataata aggattcagt gcacggataa ctgtccagtc ttgactgaag
     8941 ttcattaatc ccattacgcc taaaaaagta aaccaagcga acatgattgg tccgaatgct
     9001 tttccgacaa tatctgtacc aaagcgttgg accatgaata aaacaaaaat aataactaaa
     9061 gtgataatga cgatcacgtt ctggttatta ccgaaccggt caaaaaactg cggaattcct
     9121 cgcaagcctt caatcgcagt cgttaccgtc acagcagggg tcaatacccc atctgctagt
     9181 aaggcagctc ctccgatcat tgcaggaatg atcaaatatt tcccaccttt tcggacaagt
     9241 gtgtagagtg agaaaattcc accttcacca tggttatctg cattcaatgc aatcacgaca
     9301 tatttgattg ttgtcagtag agtcaatgtc caaaaaatca atgagactga tccgataaca
     9361 aaatcaggat tgattcctga caatccgcca ttatcttcaa caatcgattt cataacgtaa
     9421 agcggacttg tcccgatgtc tccgtagacg actcccatcg ctacaagcag gccagccatt
     9481 gatatctttt tggtattgtg cgttgttgcc acgtttttcc ctcatttctt gttatatgga
     9541 tatcctttag gtaagcttga atgatagatg atattcttat ccatgacacc cgctttccta
     9601 aattgcttct aattatccaa gtaacacact gaagtgtcaa cttatttgaa taaaattatt
     9661 ctttctttgg acgttctctg atacgtcttg ccacaaatga ataaagagca aggcaaaaaa
     9721 ttgcacccaa taatgctggt atcaaataaa aaccgccgag ttccggtccc agtggtccta
     9781 gtaaaaattc gccgagccaa ctacctaaaa aaccgataat gatatttcca gatacgcctc
     9841 ccgggacttc ctttcctaaa attgcgccag ctatcaaacc taatataccg ccgataaata
     9901 acgaccataa aaaattcagc atctcctttc cctcctttat ccatatttct cacttttatt
     9961 ttacgcttta ttcagaagga ctccaaaaat atgcgccaag aaactccaga aaaaaagaag
    10021 ttgtaacaaa agtgttcagc ttatttccgt agaagctgct tctggaacac cgtgaatcag
    10081 aggttgtgaa atcagcggtt tgacctgagt attagagaaa caaacggaaa aatagttcct
    10141 catattttgc cgttttttga gctaatatcg agggacagct gattgaacat cgtttattgg
    10201 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa cagccaaata aaaacagctc
    10261 ctaatgtttt taacttggct ggacttatta ccgcagatgc aagcttttga acaccgttta
    10321 ttcgaaaaaa aaggactgtg acacggcttt tgtcacagtc cccttttttc gaataattat
    10381 tgtccccctg tcaccttcac gatcacaagt acattattcc tatgaattaa attatttttc
    10441 ttcatttttc gttgaaccat cttcgttcaa cattgctttt cttgacagat ttacgcgtcc
    10501 ttgtttatcg atttcagtta ctttcacaag gatctcatca cctagtttaa caacatcttc
    10561 gactttattg acacgatcgt ttgctaattg ggaaatatgg acaagtccat cttttccttt
    10621 gatcaagttg acgaatgcac cgaatttttc gatacggaca actttcccta gataaacttc
    10681 accaactttg acttctttgg tcaaatcttc gatgatcttg atagcttttt tgatcatttc
    10741 tgcatcagct gaagcaatac ttacttttcc atcttgatcg atatcgattt tcacaccagt
    10801 ttcatcgatg attccgttga tcgtatcgcc gcctttaccg ataacatctt tgattttagc
    10861 aggttcgatc tggatcattt cgatttttgg tgcatattgg cttaattctt cacgtggagc
    10921 agcaatcgtt gaagtcaatt cttctaagat ttccatacgt gcttgttttg cttgagttaa
    10981 tgcttcagtt aggatttgtt ctgtgattcc ttgaatcttg atatccatct gtaaagcagt
    11041 gataccatct tttgtaccgg caactttaaa gtccatatcg ccaagatgat cttccagccc
    11101 ttggatatca gttaggatcg tatagttttc accatcagaa acaagtccca tcgcaatacc
    11161 ggcgactggc gctttgattg gtacacctgc atccatcaat gctaatgtgc cagcacagat
    11221 acttgcttgg gaagaagaac catttgattc caatacttct gctactaaac ggatcgtata
    11281 aggaaattct tcttctgaag ggattacttg tgctaacgca cgttcaccta atgcgccatg
    11341 tccgatttcc cgacgccctg gagagcctgc acgtcctgta gaaccaacag aaaattgagg
    11401 gaaattgtag tggtggatga atcgtttgct gacttccacc cctaaaccat caatgatttg
    11461 gtgctctcct agaggtgcga gtgtacaagc tgataaagct tgtgtttgtc ctcgtgtgaa
    11521 caaacctgat ccatgtacac gtggcaataa gctgacctct gatgacaatg gacggatttc
    11581 atccaattta cgcccgtcag gacggatctt atcgatggtg atcaattcac gtacgacatc
    11641 tttttcaaga tcttctgtga tttgtttaac ttctttcaac aagtgatctt catcttcatg
    11701 acctgcaaat ttctcagcat aagcttcgcg gatatcgatc ttcacctgct cgatattatc
    11761 ttcgcgagca agtttttctt cagtcattac tgctgctttc attgtagcgt aagaagcatc
    11821 aaagatttct tttttcaata cttcatcaac ttgcagtaaa gtcacttcca ttttttcttt
    11881 ccctactgct tgaacgattt cttcttggaa agcaactaac tctttgatag cgtcaaaacc
    11941 aaacagcaat gcaccaagca tgtcttcttc tgaaacttct ttcgcgccgc tttcaaccat
    12001 attgatggct tgtttcgttc cggcaacagt caattcgata tctgtttgtt ctgcttgttc
    12061 tactgttgga ttcaagacgt actcgccatt tacacgacca acttctacgc ctgcgattgg
    12121 gccgtcaaat gggatatctg agattgatag tgctaaagaa gaacctagca ttgctgccat
    12181 agctggcgag cagtctgttt ctacactcat cacgatgttc gttacttgga cttcattacg
    12241 gaagccttct gcaaacatcg ggcgaattgg acggtcgatc aaacgggcag tcaatgttgc
    12301 ttccgtactt ggacggcctt ctcgtttgat gaaacctcca gggattttcc ctgctgcata
    12361 cattttttct tcataattga tcgttaatgg gaaaaagtct gtatcttttg cttctttaga
    12421 ggccacagct gcacttaaga ctactgtatc tccataacgg actaaaacgg ctccgtttgc
    12481 ttgttttgct agctgaccga cttcgatttg taagggacgg ccgccccatg tcgttttgaa
    12541 tacttgtttt tctgacataa cttctccttt tccttctcgt gaaagcaaga cgctactaaa
    12601 caaatgaaaa ctgccgaaag ccgtcttttg agcagctttc atttggttag taggagtcca
    12661 tctagcttga tcacggaatt ttacttaatt gatatacgag taaaaaaagt gagattcatg
    12721 tgaatcccac tctttttgac taaaattaac gacgcaagcc taaacgtttg atcaattcgc
    12781 ggtaacgttg aacgtcagtt ttacgtaagt aagctaacaa gttacggcgg tgaccaactt
    12841 ttttcattag gccgcggtat gaatgatggt cttttttgtg cacgcgtgca tgttcattca
    12901 agtggttgat ttcttcagtt aatacagcga tttgtacttc tggagaacca gtatcccctt
    12961 catggcgagc gtattcgttg atgatttcat tcttgcgttc ttttgaaatt gccatttctt
    13021 tcacctcttt cgattattcc cctgcgactg agtaaaacgt tggtgattcg cttaaccaag
    13081 tggcaggtca tgtgcctaga gcacacagct atactttacc tgaaaccggg ttgaaaatca
    13141 agtagttaat cattaagaaa aggtagtttt caaacaatct tactactact tttccatttt
    13201 tgtggtaaac tataatgcga aaatagactt gaaacacagt cttttaagaa aggggtacat
    13261 attccatgat tacaatggat gatatcattc gtgaaggaaa tccgacctta cgcgaagttg
    13321 caaaagaagt ttctcttcca ttaagcgaag aagatatctc tttaggaaaa gagatgcttg
    13381 agttcttaaa aaacagccaa gatccaatca aagctgaaga actacactta cgaggcggtg
    13441 ttggcttagc tgctcctcaa ttagatattt ctaaaagaat cattgctgtt cacgtaccaa
    13501 gtcctgaccc agaggcagat ggaccttctc taagtactgt tatgtataat cctaaaattt
    13561 tgagccattc tgttcaagat gcttgtttag gggaaggtga aggctgttta tctgttgatc
    13621 gagaagtacc aggatatgtt gttcgccatg ccaaaatcac tgtgtcttat tatgatatga
    13681 atggtgaaaa acacaagatt cgtttgaaga attatgaatc gatcgtcgtt caacatgaaa
    13741 tcgaccatat caacggtgtg atgttctatg accacatcaa cgatcaaaat ccatttgcac
    13801 taaaagaagg cgtattggtg atcgagtaga gaattggcag gttccttcga tactatgcta
    13861 caagaaagct ttataaaaaa ggggagtgct caaaagctgc acttcccttt tttatgtttc
    13921 tccactaatc gaaatttaga tagacatcta gaaatcgcaa ttccaaaaaa ttctgtgtat
    13981 tggatacgca tcttttctta ttttttctta tttcttccat atgggtgttt atctcagaga
    14041 tgatagaata gtttctagga taaaaagtag cagctacgat gttatccttc gtagaggatt
    14101 cctgcttcag ctagtttttg tttgatgttt ttcagcatat gcttttctgg gtagcggatc
    14161 gtatgcagat ggatgccgcc tgttaattcg gataacaaag tagcattact tttttgataa
    14221 cgtctcataa aggaatcgat atcttgtggc gttttgatgt gcaaagttcc tgctaactca
    14281 ccgtaaaggg gatgttccac aattacatca atgatttcac caccattttc aacgattagt
    14341 ctcagttctt cttctgtttg atcttttcca tgctggacag cgattttact gatgtgccca
    14401 gtattggttt cttctgtttc gaggatataa cctcttgctg ttgccacgat acttttacct
    14461 gcagctcgta gtaaagcaat atcgcctaca atgatctgac ggctgactcc aaaagttttc
    14521 gcaaacttgc tagcactcac tggtttttct gtatttgcca aggtttgcat gatttgtttt
    14581 cgccgtgtct ctccttcgat cacacttatt cctccttcat tttgttttgg tattcgcgcg
    14641 agtgtttcat caaatcaaca tacgtgtctg ttattgtttc cttatatccc ttatttttca
    14701 gcagactaac aactttatcc aatacttgct gctctctttt tgcatcataa actgcttggt
    14761 tttcttcttt tttcacttgt cctattttag ttaccgtatc cattcttttt tctaataaag
    14821 caaccaactc tcgatcgatc atatcgatct tatgacgtaa tgtttcaaga gactcttctg
    14881 ttagttcttc ctctactgtc ttgatgatac gagcaggatt tcctgcaatc actacattgt
    14941 cgggaaaaga tttggtcaca accgaacctg cgccgactac tacgttgtcc cctagagtga
    15001 cacccggcgt gacgataact cctccgccta accaaacatt attaccgatt ttaatgggtt
    15061 ttgcgtactc cagaccgctg ttacgtttca ctggatgtag tggatgcgtc gccgtgtaaa
    15121 gctggacatt cggagcgaac atgcagttat caccgatttc aatcgtgctg acatctaaaa
    15181 atgtacaatt gaaatttgca taaaaattct ccccaacaaa tatattatat ccgtaatcaa
    15241 aattgataac tggctccata tagatttttt tccctgtacg gccaaaagtc tctttcaaca
    15301 attgggatcg cagttccgct gattctgctt ggttgatgat ttggctttga cttcttgcaa
    15361 acatacggtc ggcaaccagc tcaggatcat tggcaaaata aagctcgcct gcaatcattt
    15421 tttctttttc ggttttgaat tcagtcataa tctgctccta attcttgtat ttttttgaaa
    15481 acaaccgctc cgtcttcttg aataaacgta taaggctccg ctaaataaac aggcgtttga
    15541 ttgattacta agattttggc tgacggctgg cgaaactgta ccagatcaca aaacggatga
    15601 acttgaaaac tcgttccaac aatgacgatc agctctgctt ggctgacggc ataagccgcc
    15661 tgctccatga ctccatcttg aaacccttca ccatataaca cgatgtctgg gcgtatttgt
    15721 cctccgcatg cttgatgttt gtcgctgtga agatattctt tccagtctac cgtttgttga
    15781 cattttcgac aatgacattg atacagactc ccatgaaaat tgacgagttg cgggctttta
    15841 gcctttgcgt gcaaaccgtc gatgttttgg gaaacaaccc aaacattttt ttctttcgct
    15901 agatgtgcta tttccaaatg aatgatattg ggttttgctt caggatgata aatctttttg
    15961 acaaattgat agaatttatc cggttcattc accattgctt gatgactcag caagtattcc
    16021 ggctgctcca tcccgtggta gattcctgac aaagatcgat agtctggcac tccagaaggt
    16081 gtggataccc ctgcaccggt caaaaatgta atttgctgat aagctaatat ctcttttgct
    16141 gcttcgtcta ctgttaattg tttcatgttt tgtcacctca ctttccatta tagcccatac
    16201 tcgaacaaat ttattgttga aaagtcagaa aaatgggaat aaaaaagtgt tatgggcatt
    16261 tgagcaattt atcacaatgt tcactgacaa aagaatgaaa acgtgttaca atactgctgt
    16321 ataaaagtga ggtgaatcga tatgttacca ttctttattt attggggcgt tattctggct
    16381 tgtatcgcct ggttagcgtt aagcgtttat ttctctgttt tttatcttgt acgcaaagaa
    16441 aatggaaatc tttgggcttt tgctttattc aacgtgattg ccgcaattgt tttagccatt
    16501 acattagccg tatatcgaac atggggctgg ggaatcaccc aatactcaag tttgatttat
    16561 ctaatattag ccatttatgg cgtcgttgtt cttttgcaag cgatcctagg acgtgagcca
    16621 aagaaagcgg cagcttaaat gatcgagatg gaactggaca caggaaattt tctgttctag
    16681 ttcttttttt attgtattac atacattttt gtgtataatt atactatcgt ttgcaatgga
    16741 tagatgattc acattttgat tgttttttcg gtaaaagtaa agatttctat atttatcagt
    16801 attttttcaa aaaccagcag ttatagattg acaaagcctc tcgagtatcg taaacttttt
    16861 ttgtataaat aactgaatac gagatgcctc aatcaatgag gtagaggtcg cgagaatcat
    16921 taaccctgtg gagtgaagca aacatggaga agcaggaata gggaggatcg ccgaaatgtg
    16981 cacttttttg cttggagtgt atgttgggac gcaagttaag agcttgcgga ctgtcttagc
    17041 agtgatgcta agttgcgcta tgttttgtga gaggtttatc accttatgga attttagcgg
    17101 cctttttata tctataaatc ggtcgctttt tattttttca gataaagctt taccgcattt
    17161 catctagacc gctcgtgtat catataatca gaaagggaga aaaaaatgaa aaaaaatatg
    17221 gtttcagttg ccgtactatt tgcttttgtc ttctcactat tcagtgtggg atctctggca
    17281 aatgcagatg aaaaaacacc gagcgatgag tttcgcgtag gtatggaggc tgggtatgca
    17341 cccttcaact ggtctcaaca aacagatgag aataatgcct tgccaattca aggccaaaat
    17401 agttacgccg gcggttatga tgttcaaatc gctaaaaaag tagcagatgg tttagggaaa
    17461 aaattagtca tcgtccaaac gaaatgggat ggtctggctc ctgcccttca atcaggcaag
    17521 atcgacgcga tcatcgccgg aatgagccct actgctgaac gacgaaaaga aattgatttt
    17581 accgatcctt attacgaatc acaattagta gtagtagttc aaaaagatag caaatatgca
    17641 aaagcaaaaa gtttagcaga tctttctgga gcaaagatca ctgctcagtt aaatacattc
    17701 cactatactg tgatcgatca gataccagat gtgcagaaac aacaagcaat ggataatttc
    17761 tcagcaatga gaacagcttt agcttctgga atgatcgatg gttatgtcag cgaacgtccg
    17821 gaaggtgtaa cggcaactag tgtgaacaaa gacttggaaa tgcttgaatt cagcggtgca
    17881 gatggcttcc agacaaaccc ggaagatgtt cagatttctg ttggtatgcg taaaggcgac
    17941 ccggatatcc aaaaagtaaa ccagattctt tctggcatct ctaacgatga acgaaccaaa
    18001 attatggacc aagcagtcaa agatcagcct gctgcaactg atagtgaaga aaaacctggc
    18061 atcatttctg atttcactac gatttggaaa caatacggca atatgttctt acgaggtgct
    18121 ggacttacct tgttcatcgc attgatcggg acagttgtcg gtactacact tggattactg
    18181 attggtgtct tccgttcggt acctgaatca ggaaatgcac tgactcgctt cttccaaaaa
    18241 atcggaaatg cacttctttc tatctatgtc gaagtattcc gcggaacacc gatgatggta
    18301 caagcaatgg tcatcttcta cggtctggca ctcgcttttg gcatttcatt gaaccgaacg
    18361 attgccgctt tgtttattgt ctctctcaat actggagctt acatgacaga gatcgttcgt
    18421 ggggggattt ttgcagtaga tgaagggcag ttcgaagctg cgcaagccat tggtatgacc
    18481 catggccaaa cgatgagaaa agttgttatt ccacaagttt tacggaatat cttgccagca
    18541 acaggaaatg agtttgttat caatatcaaa gatacagcag ttttaagtgt aatcggcgta
    18601 gctgatttgt tcttccaagg aaactctgct tccggtgcga atttccaatt cttccaaaca
    18661 tttacgatcg tcgggatcat ctacttagtc atgaccttta cgatcaccag aatcctgcgg
    18721 gcggtagaaa agaaaatcga tggtccttca gcttatacca agcttgaaga aatcgacaat
    18781 tcaaatttgc aggaataagg aggaagaatc atgaatgaaa aaattattga aatcaaacat
    18841 ctaagaaaaa gcttcggtga aaatgaagta ttgaaagata tcagcgtaac agtcaataaa
    18901 ggtgaagttg tgacgatcat cggttcttcc ggttctggta aatctacttt cttgcgctgt
    18961 atcaatttac tggaaaaacc aacaggcggc gagatcattt ataatggaga aaatgtatta
    19021 gctcctaaat ataatttacc taaatatcga acgaatctag gtatggtttt ccaatccttc
    19081 aacctgttca acaatatgaa tgtcttggaa aactgtatgt ctggtcaaac aactgtcctg
    19141 aaacgagata aagaaactgc caaaaaagta gcaatcgaaa atctggaaaa agttggtatg
    19201 gatcgttata tcgaagccaa accagcacaa ttatctggtg gacagaaaca gcgtgtcgca
    19261 attgctcggg cattatcaat gaatccagat gtattgctat tcgacgaacc tacttcggcg
    19321 ttagaccctg aaatggttgg cgaagtgctt aatacgatca aagacttagc acatactggc
    19381 ttgacaatgc ttatcgtgac gcatgagatg gaatttgcaa aagatgtatc tgaccgtgtc
    19441 atttttatgg ataaaggtgt gattgcagaa gaaggtacac cagaagatat ctttgtccat
    19501 ccgaaagaag aacgtacaaa agaattcctt tcacgtattt taaatgctta ggatcaatag
    19561 ctgattggga caaagagctg cggtgtttac cgcagttctt ttttatgaat tactcataaa
    19621 aggtaaattt ctgaaaattt tggaatctta gttgtgtttt tcagaaaact ctcttaatat
    19681 ggaagtaatt aaactttttt agttaggatg atttaaaaat gaatgaagag caagaactgc
    19741 agcgtggact gaaaaataga catgtccaac tgatttctat tggcggagcg attggaacgg
    19801 ggttgttttt aggttccgga aagtctatcc agctagctgg cccttcgata ttgctcgcct
    19861 atcttattac tggatgcatc tgttttttca ttatgcgtgc gttaggcgaa cttctgttat
    19921 caaacaccaa taaccattct tttctggatt ttgttgcaga atatcttggc aaaaaagcgg
    19981 ctttcatcac tggctggacg tattggttct gctgggtatc gattgcaatg gccgatttaa
    20041 cagccatcgg aatgtatgta cgctactggg cgcctggtgt tcctcagtgg cttcctgaac
    20101 tgattgcatt aggtcttctt ctttgcttga atatggtggc tgtctcattg tttggtgagt
    20161 tagagttttg gtttgcattg atcaaagttg tagcgatcat tgcctttatc ctcgtaggag
    20221 cttatatgat cctcacccac tatacgacag atattggcac agccagtatc acgaatctat
    20281 ggtctcatgg cggattcttt ccaactggtg caaaaggatt tatcttagct ttccaaatgg
    20341 taacctttgc ttttgctggg atagaattag ttggattagt cgctggagaa acagaaaatc
    20401 ctgaaaaagt gttgccggaa gctatcaata atattcctat caggatcatc ttattttact
    20461 taggatctct ttttgtcatt atggcaatct atccatggaa cagcttgaat ccagataaca
    20521 gtccatttgt agaagtattt tcggaaattg ggatcacgat tgctgcttcc ttgatcaatt
    20581 tagtcgtatt aagtgcagca gcttctgcct gcaacagtgc aatatatagt acaggacgta
    20641 tgcttcgttc tttagcgcaa gaaggcagtg cccctaaaaa attcaaaaag ttaacaacac
    20701 atcatgtgcc agggaatgct ttgacatttt ctactatcgt cattttcatt tctgttatct
    20761 tgaattacgt gatgcctagt gaagtattca cactagtaag cagtattgcc actacttgtt
    20821 tcttgtttat ctggagtatg cttgtctaca cgcacatgaa atatcgtaaa agtatcctcg
    20881 gcaaaaaagc tcattcattt aaaatgccgt tgtacccatt tagtaattat ctggtatttc
    20941 tttacatggc ctttgtgtgt gtcgtcttgt tcctaggaaa agatacgaga atcgctttat
    21001 tactaacacc tgtgtggttc ttgcttctat tactaatcta tcatatgaaa tatgaacgta
    21061 agaagtaaga aagtagttgt gacagaagtg gtcagcttcg agaaataaga aggaatttac
    21121 gaaaattgct tcttaaattt ttgtgaaatt tcggcttatt tcttggagat aaaagcttct
    21181 gtcccagcct cttttctatt tccccggaaa caaccgctga taataggcat ctgctttttt
    21241 ataggaaaaa atccaaatac cagatacaaa aaaacgtccg aataacccga tcaacggtac
    21301 aaaaaagaca ccggcaatca agcataagtt aatcatcatg gagataagac ttcgacggtc
    21361 ctttgacaaa actctcacga catgggaatc tggtttattt gctttcatga tttgcctcat
    21421 caagatacca aaagaaaaac tccacatcgt gtatactaag acataagcca tctcaggcca
    21481 aaaagcttca gggttacgcc cgacccaact agtaacagaa ggacataaag tcagccaaaa
    21541 gagcaaaaag atattcgacc agaccactcg gtaattaatt cgttctgcca tctgaaatat
    21601 acgatgatgg ttcacccaga tcaccgcaac aaatataaag ctgatggtat aagctaataa
    21661 tgtatttcgg atgacccata aagaatgaaa gctagatgta ttagggatct tgatctctaa
    21721 tacaagaact ctcaaaacaa tggctaccac tccatcagta aatgcttcta atctcgactt
    21781 tgtcacgatg attcctcctc agatgattca gcgcttttat tttatcactt acgttattaa
    21841 ataaccatcg ttttatatag ggaattttct gaaaattgtt gcatattttt cgaaattttg
    21901 ttacttttaa aaaaaccgta aaggagttat gtatgacaaa aaaattatca tttaaagact
    21961 atctttttat aggctccatg cttttcggtc tattttttgg tgctggaaat cttatctttc
    22021 ccgttcattt gggtcaagaa gcaggcgctg ctaccttttg ggcgaatcta ggtttcttgg
    22081 ttaccggtat cggtcttccc ttcttagggg tgattgccat tggtgtatcg aaaagctcag
    22141 gtgtatatga attagcaaaa cggataaatc aaacatatgc gatggttttt accatcttgc
    22201 tttacttagt tatcggaccg ttttttgctt tacctcgcct tgcaaccaca tctttcgaga
    22261 ttggtttagc accatttatc gatcctagca gacagacgct atatctagca attttcagta
    22321 ttgtattttt tgtgctggct tggtggtttt caagaaaacc gacaaagata ttagattatg
    22381 ttggaaagtt cctgaatcct gcatttttgg ttttattagg tgctttgctt ctccttgctt
    22441 ttttgaatcc actaggttct gtaaatcaag cgcctattca gccaaattat caagagcatg
    22501 cttttttcac agggtttact caaggatata acacattaga tgcgcttgct gctttagcat
    22561 ttgggatcat cattgtcaca acaatccaaa acatgggtgt aacaaaacct gctgaaatcg
    22621 ctaaagacac gatcaaatct ggtgcgatca gcattatatt gatgggaatc atttatactt
    22681 tgctcgctta tttaggtgct atgagcttag gtagctttgc tctaagtgaa aatggcggga
    22741 tcacgctggc tcagattgct gatcattatt taggtactta tggcagtatc ttattagcat
    22801 ttatcgttat attagcttgc ttgaagaccg gtattggttt gattacagct tttgcagaaa
    22861 cattcacaga tttgtttccg caaagaaatt acgcattctt tgttgcactt gctagtatcc
    22921 ttccttgttt agcggcaaac gtaggcttga cgaatatcat tcaattttcg ttgcctgttt
    22981 tgatgttcat ctatccatta gcaatgacac tgattttact agtgttagtt ggtcctttgt
    23041 ttaagcagcg tacggctgtt tatcgaatga ctacttattt tacattgatt gcttcgattt
    23101 ttgatgggtt gaacgcttgc ccagaaacta tcaaacaaac acctattgtt caaaatcttt
    23161 tatatgttgc ggaaagctac ttgccgtttt tcaaattggg gatggggtgg atcgtaccag
    23221 cagttatcgg ttttgttatc ggactgattt ggagtttcgc taagaaagaa gaagtagcag
    23281 attgataaaa gaggttgtga aagagacgtt cagccttgag caataaaaag taattttgaa
    23341 aaatggcttc tcacattttt gcataaattt aacttaatac cgagaaactg acttttgaac
    23401 accgtttatt gagaggtcgt gaaaaagccg cttagtttca agtattaaga aatattttgt
    23461 gaaaatggct tctcacattt ttgcataaat ttgacttaat accgagaaac tggcttttga
    23521 acaccgttta ttgagcgtct ataaactgac ctgagtatta gagaaacaaa acggcaaagt
    23581 agctccatat actttgccgt tttgttttga actaataccg aagagaagct gatcaaacaa
    23641 catttctttt ataatataga ttttagacat atatatgata taaaaatagt tttcgcaaaa
//
LOCUS       DACDEG010000033        23458 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.035, whole genome shotgun sequence.
ACCESSION   DACDEG010000033 DACDEG010000000
VERSION     DACDEG010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.035"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(320..520)
                     /locus_tag="IV142_001374"
     CDS             complement(320..520)
                     /locus_tag="IV142_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8485043.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(825..2057)
                     /locus_tag="IV142_001375"
     CDS             complement(825..2057)
                     /locus_tag="IV142_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP8485044.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2311..2880
                     /locus_tag="IV142_001376"
     CDS             2311..2880
                     /locus_tag="IV142_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8485045.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLC
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3058..3498)
                     /locus_tag="IV142_001377"
     CDS             complement(3058..3498)
                     /locus_tag="IV142_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP8485046.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3656..4420
                     /gene="map"
                     /locus_tag="IV142_001378"
     CDS             3656..4420
                     /gene="map"
                     /locus_tag="IV142_001378"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP8485047.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4452..5375
                     /locus_tag="IV142_001379"
     CDS             4452..5375
                     /locus_tag="IV142_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP8485048.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5451..6590
                     /locus_tag="IV142_001380"
     CDS             5451..6590
                     /locus_tag="IV142_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP8485049.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6583..7383
                     /locus_tag="IV142_001381"
     CDS             6583..7383
                     /locus_tag="IV142_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8485050.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7383..8210
                     /locus_tag="IV142_001382"
     CDS             7383..8210
                     /locus_tag="IV142_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8485051.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8188..8922
                     /locus_tag="IV142_001383"
     CDS             8188..8922
                     /locus_tag="IV142_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8485052.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            9022..9888
                     /gene="rfbA"
                     /locus_tag="IV142_001384"
     CDS             9022..9888
                     /gene="rfbA"
                     /locus_tag="IV142_001384"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP8485053.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9902..10474
                     /gene="rfbC"
                     /locus_tag="IV142_001385"
     CDS             9902..10474
                     /gene="rfbC"
                     /locus_tag="IV142_001385"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP8485054.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10496..11524
                     /gene="rfbB"
                     /locus_tag="IV142_001386"
     CDS             10496..11524
                     /gene="rfbB"
                     /locus_tag="IV142_001386"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP8485055.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11622..12473
                     /gene="rfbD"
                     /locus_tag="IV142_001387"
     CDS             11622..12473
                     /gene="rfbD"
                     /locus_tag="IV142_001387"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP8485056.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12508..14541
                     /locus_tag="IV142_001388"
     CDS             12508..14541
                     /locus_tag="IV142_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485057.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14585..15865
                     /locus_tag="IV142_001389"
     CDS             14585..15865
                     /locus_tag="IV142_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP8485058.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            16075..16881
                     /locus_tag="IV142_001390"
     CDS             16075..16881
                     /locus_tag="IV142_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8485059.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16893..18113
                     /locus_tag="IV142_001391"
     CDS             16893..18113
                     /locus_tag="IV142_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8485060.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            18103..19689
                     /locus_tag="IV142_001392"
     CDS             18103..19689
                     /locus_tag="IV142_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8485061.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19728..21866
                     /locus_tag="IV142_001393"
     CDS             19728..21866
                     /locus_tag="IV142_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8485062.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAESTHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(22234..23217)
                     /locus_tag="IV142_001394"
     CDS             complement(22234..23217)
                     /locus_tag="IV142_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAP8485063.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
BASE COUNT         7392 a         4010 c         4810 g         7246 t
ORIGIN      
        1 taaaaatctt tgataaatct attcgaaggg tcaaatttaa accataattg tgtttttcct
       61 tccatcagca taaaagttca cctcaaaaag agtatacgaa cgaatgttct ttttgtgaag
      121 gcggaattta atatactgaa taatagaaaa caagtacttg tgccaaatta tgtgccaaaa
      181 aaattcgaat tcaacaaaat gtaaacaaat agaaaacctt ctatattaag atttcctgca
      241 ataacaaaca caacagaaga cgtgtaatag ttagtcagga acgtacaaat aacccctgta
      301 tcctttgcgg tacagggggt tattttgtat aagtagacat tgtagtttct aaactgctat
      361 gtcctaaacg tttcgatacg ctaagtatat ttactccttg ataaagtaaa atagatgcat
      421 gcgtatgtct tagtccatca ataaacagtc gaagagaaaa tagggaaaaa ttgattacta
      481 aagaaaaact tactaggtcg acggtgtagt tgattttcat acttcaatag attcgaatta
      541 aacgatttcg attggcttat accagtccta tccaacgaaa gatttatcgt tcttaactct
      601 agcggtataa cttgaagctt tacgtaaagc caatgggaat gaactggaga tgacttttta
      661 ttatttttag taatagctga tgggttaata cttacaactt tccttttcaa agtaccatat
      721 aaaaaaacgg tggggttgtt tagcgaaata aaggatctaa gccaatagct tagatccttt
      781 attctatgct ttatattatt ttttgcatgt gtgcgaaagt atactcaagc ccaatcgcca
      841 tttcggaaaa tcggtactgt agagccatct tcacggattc cgtcgatatc catcttgtca
      901 gatcctacca tgaaatctac gtgggtttga cttctattca atccagcttc tgctaattct
      961 tcatcggaca tttctgtacc gcctttcaca ctgaaggcat aagcagaacc tagtgctaag
     1021 tgattcgatg cgttttcatc aaataatgta ttgaaaaaga tgatgccaga ttgagaaata
     1081 ggtgaagggt caggtacaag tgcgacttct cctaaacgac gcgcaccttc atctgtatca
     1141 agcagttttg ctaagacatc ttctccttgt tcggcagaaa aatcaacaac ttttccatct
     1201 ttaaaagtaa atttcatacc cgagatgatg gtgccagcat aactcagtgg ctttgtactt
     1261 gaaatgtaac catctactcg atgactgtca ggtgctgtga acacttcttc cgtcggcata
     1321 tttgccatga atttttctcc acgagcatta tagcttccag cgccttccca aagatggttt
     1381 ttaggcaatc cgataatgat atctgtaccg ggagcagtat agtgaagggc agaaaattgt
     1441 tctcgattca attcttctgc tttttttgct aatttttcat catgcttctt ccatgctaag
     1501 acagggtctt cttcatagac acgagtcgtt ttgaaaatct gatcccataa tgcctcaact
     1561 tgtttttctt ctggcagttc tgggaagact tttgctgccc attgtttacc agccgcagcc
     1621 acgacagtcc aactgacttt atttgcttga gttgccttgc gtaaattcat tagtgctttg
     1681 ccattcgctg attgataaga cgctactcga tggctatcta caccagcaaa tgcatcggga
     1741 tcggcagaaa caacgctgat cctgcttgct cctttttcaa gccattcatc cgcttgatcg
     1801 attttagatt gtggtacatt ctcaatacga tccgttgcag catgtaaaag aaattctctt
     1861 tggatctggt cgtcagtcca ttgaacgata acctcagcgg ctcctagttt ataagcttct
     1921 tgtgtgatca atcgagcaag gggtgcctgt tccacgctga tctgtaaaac aactgtgtgt
     1981 cctttttctg tggctacgcc agtttctgca attaagcgtg catatttttt tagaagttca
     2041 ttaaaatcag ataacataat aaaaattcct cctcaccatt ttttctggta actattagca
     2101 tgataccatt taattatgat tcaaacaatg aggctttaaa taaaaaaagg aggttatttt
     2161 tttaaggtta agtttcatga tatatgttac tacttgctat ttcagcttgt gaagaaaatc
     2221 aaaaactaat tttgcatatc aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     2281 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     2341 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     2401 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     2461 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     2521 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     2581 gcaagaagag agaagaagtt gtatcgttca ctgtgcttag aagaatatgg tggaggaaaa
     2641 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     2701 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2761 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2821 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2881 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2941 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     3001 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     3061 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     3121 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     3181 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     3241 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     3301 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     3361 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     3421 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     3481 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     3541 taccaaaaca tcattgaaaa gaaaataaaa cctaaagaca cctggggaat gtcatttggc
     3601 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     3661 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3721 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3781 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3841 gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3901 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3961 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     4021 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     4081 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     4141 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     4201 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     4261 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     4321 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     4381 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     4441 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     4501 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     4561 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     4621 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     4681 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4741 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4801 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4861 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4921 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4981 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     5041 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     5101 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     5161 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     5221 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     5281 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     5341 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     5401 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
     5461 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
     5521 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
     5581 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
     5641 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
     5701 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
     5761 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
     5821 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
     5881 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
     5941 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
     6001 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
     6061 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
     6121 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
     6181 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
     6241 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
     6301 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
     6361 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat
     6421 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
     6481 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
     6541 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
     6601 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
     6661 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
     6721 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
     6781 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
     6841 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
     6901 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
     6961 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
     7021 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
     7081 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
     7141 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
     7201 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
     7261 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
     7321 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
     7381 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
     7441 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
     7501 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
     7561 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
     7621 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
     7681 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
     7741 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
     7801 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
     7861 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
     7921 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
     7981 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
     8041 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
     8101 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
     8161 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
     8221 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
     8281 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
     8341 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
     8401 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
     8461 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
     8521 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
     8581 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
     8641 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
     8701 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
     8761 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
     8821 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
     8881 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
     8941 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
     9001 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
     9061 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
     9121 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
     9181 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
     9241 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
     9301 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
     9361 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
     9421 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
     9481 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
     9541 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
     9601 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
     9661 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
     9721 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
     9781 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
     9841 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
     9901 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
     9961 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
    10021 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
    10081 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
    10141 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
    10201 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
    10261 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
    10321 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
    10381 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
    10441 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
    10501 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
    10561 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
    10621 agagaacttg gccggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
    10681 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    10741 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    10801 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    10861 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    10921 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    10981 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    11041 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    11101 agtaacgagt atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    11161 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    11221 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    11281 attgatctta gaaatgatgg gccaaccaaa agacgcatac gaccatgtaa acgaccgcgc
    11341 agggcacgat ttgcgttatg caatcgattc gacaaaattg cgagaagagc taggttggca
    11401 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    11461 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    11521 ataatacaga ggaaatttca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    11581 tgtctctttt ttaaaataaa aatcaaggag cggataacca tatgttttta ttaactggag
    11641 gaaatggaca attaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgccatatg
    11701 tttcaacaga tgcaaaagac ttggatatca cagatgctga gaaaacaatg gcttatatta
    11761 cagaattaaa acccgatgtt attttccatt gcgcagctta cacagcagta gataaagcag
    11821 aagaagaagc aaaagaatta gatgagaaaa tcaatgtcga tggcacaaga aatgtagcac
    11881 ttgcagcaaa agctgtgaat gcacaattcg tctatatcag tacagactat gttttcgatg
    11941 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    12001 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    12061 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    12121 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    12181 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    12241 atcatttgtc gaacgaaaat agctgttctt ggtatgagtt cgcaaaagaa atattgaaag
    12301 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    12361 cgcagtattc agtcatgagc ctgaaaaaga cagaagcgtt aggttttgtc ataccaactt
    12421 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    12481 tttatcaaat agaatttgga gcatgatatg gaaaaacaaa caagaagtgc acgacgaaaa
    12541 gaaaagccaa aagcgaatca ggcagattcg aatttggtgc ttgacctgtt tctaaaactc
    12601 cgctatctta ttggcgtttt agtcatcgtg gtcatcgtta cttttaattt aaacggaagt
    12661 tcacttggtg tttgggataa atatgtgtcg caaagagacg atgggaaaaa aactgatgtg
    12721 atttttggac agaatcggga agtgcggtca gacgaatggc tggtgcagac acctttttac
    12781 ctctcacaag ctgaagagga cttttcgttg gtgaacgagg attattcatt aaacggacaa
    12841 aacatgatca ttgcgtataa ttctcctgta aaggatatta cggttatcgg aaaaccattc
    12901 aactggggat tctttttcct cggaagagat cgaggacttt ccttttattg ggcgatgaaa
    12961 ttagtcgtca tggtcttgct tggattcgaa gtactgatga tattaacgaa gaggaaaaaa
    13021 gctctatcgc taatcggtgc atttgcgctg acattttctc cagcagtcca gtggtggttc
    13081 atgcaacatg tgggcgattt gattttcttt actttgggac tgatgattgc tttttatcat
    13141 tatttctaca atcatgataa aaaatggcta cgaacgttga tgatgctttt ggtggttatt
    13201 ttcggtattg ggtttatttt agtgatttac ccagcccatc aagtgatgct ggcgtacttg
    13261 ctgatttttt attttgttgg cctgcttatt tactatagca agaaagtttc atgggatctg
    13321 ttggatgtag tgttgattgg aggggcagtt ttatttattg gcggagtcat ggttcatttc
    13381 tggctaacgt caaaagacgc attgttagct tctttgaata ccttatatcc aggaaaacgg
    13441 gtaagtactg gaggaaagtg gacgattggc gaattttttt ctttccttac aaattggaaa
    13501 attccttttg aagatatcaa ctattcgaat aactctgaag tggccctgtt ctatcacttt
    13561 tttccaactg tattcctagc aagtccattt gtgttatttg gcaaaaaaga tagcgaacaa
    13621 aagttactgg gacgtatttt gatgattttt tgtctgtttt ctatcttttg gattaccgtt
    13681 gggttgccaa aatggctagc agaaatcact ctgctgtctt atgttcctcc attaagagcc
    13741 atccttactt ttagttttgc ggcttgttta ttatcgatct ggtttatcgc ttatatttgg
    13801 agagaagatg ttattcctat ttggtataaa ttcattttgt tggcagtaaa tgcatggtct
    13861 tatttctatg cgattacgca ttcgaaaatg gataattatt tttcggattt tgaaaccatc
    13921 gcagttgctg tgttgggaac agtgatttta gcttttgcat tatttggctg gagacgcttg
    13981 ttttacctcg cttttgtcgc cttagttatc gtatctggct ttttcgtcaa tccagtcgta
    14041 gaaggtacag gagcgatttt tgaaaaaaca ctggcaaaag aaatccaaaa aatcgatcaa
    14101 caagatcctg gtaaagtttg gttgtctgaa gatgaattat ataacttcac cccaacattg
    14161 ggagtgaaat ccttcaatac agtacgcttt tatccagata tagctgcatg gaagaaaatc
    14221 gatccgaaag aagaatacga gaagtattat aatcggtatg ctcatacaag agcttttatc
    14281 gctaaggaag atacaaagtt cattttagat cgtccagata tgttttcggt cacgttgaat
    14341 tttgaagacg cagaaaaatt agggatcgac tatgtgatca gtaaacgacc attaactgat
    14401 tacaaccaat tatatgaagc gcagttcacg cagctatatg gaccggataa agacggttat
    14461 atgattttta aagtagaata tccggattat ccagatgtac cacaaacaca aactcaggaa
    14521 aatcagctag tcgtacagta ataggctagc tgagaataat cctgaaatat tagtagaaag
    14581 gagagtgaag ataattcaag caatcgtaca aaaatttgtc aacaaaatga gtgtcggttt
    14641 cttgcttttg ttgctgacgc tagtcttttt tgtttggaca gcaggggtag gttttccagc
    14701 aactttgttt atatataaca atagttcatt acttatcgct tttagtgtgg ctattttgct
    14761 cattttaaat gtttataaag caaaaggcgt cgattttctt tatctaggtg ttgcgcttgc
    14821 ccttttcatt ttcttttctt actttcggga attaagaaat gagtctttat ttggagactt
    14881 actgtttcct ttgattattt tattttgttt agtaattaaa tggatcgagt ggacaaaatg
    14941 ggatcgcggt atttatctga ttgtctttac ggtcttcctc ggaatcacgt tatatcgtgt
    15001 atttacagag atccaagtac cagaaggaca aagcatctgg gctccaggca attcgctttc
    15061 tgatatttgg atcaatacga atacaatcgg aagttctttg atgactcttg ctttattgat
    15121 cagtggattt gccagttctt ttgaacgatg gtatgttcga atcatcggtg ggatagcagt
    15181 cgttgcagcc tttttaggta tttgggtctg ccaatctaga ggtgcattag tcgcgttgat
    15241 tgtttttgcg atattggatc ttttacctaa atcgttgatg cgtgtcattc gtgcgccatt
    15301 tattgcctac acggttacga ttcttttagc tttgccgatt tcttatctgg cggctgtctc
    15361 tgaaaaagtc aatttgttta ccgatcgaga agatatttgg cataaattct atcagacgct
    15421 aggagaaaaa tctgaacaag ttttactagg aatgaaaaca tttatcttcc agcgaggaaa
    15481 ccaattttta ggaaaccaca atagttataa ttctattttg aatatctatg gattgatagg
    15541 atttgggatt gctgctttgc tgcttatcct ttttattggt cgcttaacgt tgaaagcaga
    15601 tttatccaac gggcagatga cttttatctg ggcctttttt gcggtgatga tgcagtcatt
    15661 tatggaggat acattgactt catacccatg ggttccgatt atttatatcc tgttggctat
    15721 ggccgttcat cgatatgatg aaccagaagt tgcggaggaa ttctatgtta tggaagaggc
    15781 atatgacgat atgacttatg atgatacatc ttatgaggaa caaccagata gcgctcattc
    15841 ttccaggatc caacgctatc attgataggc acaaaatacg agagaggctg tgacaaccgt
    15901 cgcagccttt tttggtttat tgcaattgtc tagcatcttc tgtaaaatag aaacgttaga
    15961 gaaaatcttg tatcatagaa aattaagaaa gaaataagaa cgcagctcgg taccaaaaat
    16021 atggtaaaat ggcacttgac tgtaattcac tgaaaatcgc ttggaggaac acaaatgttt
    16081 aaagatttgt atttatttat gaaggacatt tatcagaaca gaaaattatt gctacagttt
    16141 tcgatcaatg acttcaaatc aagatatgca ggatcgtttt taggaatctt atgggcgttt
    16201 atcaatccgc tatttactgt attgatttat tggctggtat ttggtgttgg actgaaagca
    16261 caaatgatcg atgcaaaata cccatttatc gtatatttag ttaccgggat gattccctgg
    16321 ttcttcttct cagatgcatt tgcatcaact acggttgttt ttcgggaata tacttattta
    16381 gtaaagaaag tcgtattcaa tattcgtatc ttgcccacga cgaaaatcct atccaatctg
    16441 tacacccatt tattcttcat tctgattggc atagctgtcg cgcttataaa tggtatatat
    16501 ccttcagcaa tgtctttaca gctgatttat tatttatttt gcatgtgtgc tttcttgaca
    16561 ggattgactt ggctgacggc atcgatccaa ccatttttac ctgatatcat gcaattaatt
    16621 acgatcgtgc tgcagttgat catgtggaca ttaccgatat tatggagccc taatcagttt
    16681 gattcaggtc ttgtaagcat attgaaatta aatccgctgt actatgttgt tcaaggttat
    16741 cgtgagtcat ttttaagtga agcttggttc tgggagcatg gaacatatac tttgtatttc
    16801 tgggttttca cgttagtaat gttcgtaatc ggctctgcag tgttccgtag attgaaacca
    16861 catttttcag atgtgctgta aggagaatag ctatgacaga atatgcaatt agactaaaag
    16921 atatcacaaa atcttataat atgtacgcaa aaccttcgga tcgatttcgt gaagccctca
    16981 atccttttaa gaaatcctat catgatgtat tttatgcgtt gaaaaacgtg aatgtagaaa
    17041 taaaaaaagg tgagatgatc ggtttcgtgg gagaaaacgg atcgggaaaa tctacaatgt
    17101 tgaaaatcat tacgggagta ctgacaccaa catccggcac gatggaaatc gaaggaaaaa
    17161 tctcagcttt gctagaacta ggatcaggat tcaatcctga gtattctggc tatgaaaata
    17221 tctatttaaa tggaatggtt ctaggatttt caagagaaga agtcgatgaa atggtcgatg
    17281 atatcattga atttgctgac atcggagacc atatttatca acctgtaaaa acttattcaa
    17341 gcgggatgtt cgttcgtcta gcttttgctg tagcaatcaa tgtgaatccc gatattttga
    17401 ttgtggatga agcattggca gtaggcgatc ttgaatttca actaaaatgt atggaaaaat
    17461 ttacagaaat caagaactcg ggaaagacga ttttattcgt ttctcatgat atcagttcga
    17521 ttcgtcgttt ttgtgatcgt tcctattggt tgaaaaacgg agaagtcgtc gaatatggcg
    17581 atacgatgga agtaacgacg aattatgaaa actttttaaa gaaaaaatca gttaaaacag
    17641 tggatcggaa taaaaaccaa gtagaacggt tcgccgctcc agatatcgtc gacgtgacga
    17701 aagcggaatt gttggataaa gacaaaaatc cggtggatat gctggaacaa ggagaaaaac
    17761 tatacgttaa agtaacttat gaagtaaaag atgatacaat caaaaatccg gttttaggaa
    17821 ttgcgctacg aacagtcgac aatttttatg tatgcggact taatactcta ctagatggta
    17881 tcaagattcc ttggaaaaaa ggagagaata tcttttatct ggagtatgaa aagttaggtt
    17941 tgttagctgg tgaatattat tttgacgttg cggtctttga agaaaatgca acggttcctt
    18001 tggtatataa gacaaagtac atgaacttgt ttgtcagcgg ttcttatatc ggagaaggca
    18061 tcgtggtctt agaccataaa tgggaagaag ggactcatag caatgaaata tgattttgaa
    18121 atggacctag atgaacaaag cagtgtggga aaaattgccg cacagatcaa accaggaagt
    18181 aaagttttag agtttggtcc cggaaacggc cgattaacga aacatttaat cggagcaaaa
    18241 caatgtgaag tgagcattgt tgagttagat aaggaattgt tcgattttgt cagcgaattt
    18301 gctcaagatg gattttatgg tgatatcgaa agttttgagt gggcaaacta ttatgctgga
    18361 cagacatttg attacgtctt gtttgcggat gttttagaac atttggtaga tcctggaaaa
    18421 acattgaaga aagtcagaga atttttgaat gaagaaggag agatcctgat tacattccct
    18481 aacttggcgc ataattctgt catgatcgat ttgttcaata accaattgcc gtgggcttct
    18541 tatggtctcc tagatgaaac ccataattct ttttatactc atgatggctt ccaaaaagtt
    18601 tttgaaaaag cgggattatt cattaatatt gaagattatt tgtacttagc tgtaggtgat
    18661 acagaattaa aatcaactta tgaagaattg ccggaagctg tacgctatga tttcaaaatg
    18721 cgaccatttg gtgaagtcta ccaatacttt ttctcattga tgaaacatcc agtagcacaa
    18781 agttccattg cagagccaca aaattctaat tatgtaaaag tgttagaagt aacccagcaa
    18841 acgaaacaag atgaaacaat ccaacaaata ccattcaata atttcacagg ggaaaatgag
    18901 acactaagtt tccccgtatc agaaacaaca gaaagaatgg tgtttcgctt cgcgcaacaa
    18961 cctagcttta tcgaattcag cgcagaagct gccggagaaa aactaacatt tatcgattcg
    19021 aatgctgtag tcaaaacagt aaatgattgt tatttgtttg atgggaaaga attaccagag
    19081 ttcgttctaa cggatatttc tgggaaagaa gtgactatcc attgtcacta tcgctttatc
    19141 ggggaattga caccaacaat gaaagaattg ttggaaacga ttcgtcccat ggctgaagtc
    19201 acaaagcagc tttctgagaa aaatgacgaa ttagaaagag aaaatcatca tcttcttgag
    19261 gaaaatacaa gactagacca tacgttaaaa acaaccacaa atcgctattg cacattgttg
    19321 gaaagtgatg aatggacaat aaaacataga ctaggacgac gtaagaaaga aacttcgaaa
    19381 aaaatccaag aaaaagaact ttctatatgt attgacgaga aaatctggga tgcagaaaca
    19441 aaaatattaa aaatcatcgg atggggaatc gctaattcgg atcgccagcc cttgtcctat
    19501 aagttatcag ctgaccaatc gccatttttc gaagcaattc ctgtttcacg agaagaagtc
    19561 aatcaagcag aacagctcgc aaaaggaaca gaagcgggat ttgagttacg aatcctttgc
    19621 gagcaagaac gttcattctt agtggaagca gtagctcaaa atggccaatc atggatcatt
    19681 gaaatgtaaa taatgattaa cttataaaga taaggagtaa acgaatcgtg caaaaaaaag
    19741 aaattgccgt atttatcgat caaatcactc gtgaacgggc cactggcgat ttgcttattg
    19801 tgggctgggc aattgatgaa gtgaccaaag aaatccctac gataaaggta gaaaaagaaa
    19861 atgtgattgc tgaatcaaca catgtcgtaa gattggatat caatcattta tataatcttg
    19921 atgttaagac ccaaagcggt ttcaaggtac gtttatcagg gaaaatgaga ggaaaagcca
    19981 tcttagactt ccaaacagcg agacatcaaa atggcatagc tgtgaaatta aacggtaaat
    20041 acccttatga tgacggcatt gaatcttcat gggaaagaaa aaaaagatta ctgaaaaaag
    20101 ggataaatta tgcaaggact catggcgtaa aaaaagcgat ccgtcgggtc aaattggaat
    20161 tgaagccagg atcgatcgac tatgcagagt ggatcagtcg tcatgagaaa ccagatttga
    20221 aagagcaaag gaaagaagca caaggatttg cgtaccgtcc attgatttcc atacttatgc
    20281 ctgtctataa cgttgaaatc aaatggctcg aaaaatgcat tgattcggta ctggatcaaa
    20341 cgtatgatca ttgggaacta tgtatcagtg atgatgcttc tactgatcca gctatccgaa
    20401 agtgtctgga atcctatcaa gcaaaagatg atagaatcaa agtcgttttc cgaaaagaaa
    20461 atggacacat cagtctggca actaattctg cattggaaat agcagaagga gaatttatag
    20521 cactcttaga taatgatgat gaattaccgc catttgcatt atatgaagtg gcaaaagtgc
    20581 taaacgtaca tccagaactg gatgtgatct acagtgacga agataaaatc gatgcagatg
    20641 gcaatcgctt tgatccgcat ttcaaagcag actggtcgcc tgatacattg atgggaaata
    20701 attatatctc tcacttaggc gtatatcgaa caagcattgt taaagaactt ggcggtttcc
    20761 gtaaaggcta tgaaggttct caagattatg atttggtcct tcgtgtgaca gaacaaatcc
    20821 ctgcagatca tatttaccac attgaccgag tcttgtatca ttggcggacg atcccaggat
    20881 caactgcaag taatggcgaa gccaaaagtt atatctatga ttccggagtg aaagcattga
    20941 cagatgccct gtctagaaga aacatcaaag gaagcgtacg tcctggcaga atttctggat
    21001 tttatgagat tgcttatgac gtcttgcaag aggatttggt cagtgtgatc attccaacga
    21061 agaatggcta tgaagatcta aaaacttgtg tggattcgat catcgaaaaa acgtcttatt
    21121 caaattatga aatcattatt gcggataacg gaagtacaga tccaaaaatg caagaattat
    21181 ttgcagaata caagcatcag ctgaaagacc gctttatcgt agaattgatt gacattccat
    21241 tcaattattc tcgaattaac aatttagcag cagaaaaagc aaacggcaaa tactttttat
    21301 tcttgaataa cgatacagaa gtgattgagc cagattggat gacggtgatg gtttcttatg
    21361 cacagtttga tcgaatcggc tgtgtaggag caaaactgtt ttatccagat gatacaacgc
    21421 agcatgcagg agtattatta ggaatcggtg gtgttgcagg gcatgcattg aataactatg
    21481 atcgaacaca ttgtggttat ttcggccgat tggtcattga cgtcaattac ttagcagtca
    21541 ctgcagcttg tatgatggtc aaggcagctg acttcaatgc tgttaacggg tttgatgaaa
    21601 cattagaagt agcattcaac gatgttgact tgtgcttgaa agtttatgaa ctgggtcgtt
    21661 acaatgtcta tgcacatcaa gcagaattgt accatttcga atctaaatca agaggttatg
    21721 aagatactcc agaaaaacaa agacgttttg ctggtgaaat caaaaaaatg caagataaat
    21781 ggccagcata tattgcgcat gatccatttt acaatgataa tttaacaaaa gacggaatcg
    21841 gcgacttttc gttgagacca gattaaacta taataaaaag ctgttatgtt gtgattcgct
    21901 cgcacataac ggctttttat agttttttgt caaaaaaagg cagaaggtgc cgactcaaaa
    21961 aaataagtcg aggttgggac agaagtgttt aactccgaga aataagaaga aattcacgaa
    22021 aattgctttt caaatttttg tgaatttcgg cttatttaag aagaagttgc ttctgctccc
    22081 accgtttatt cgtttttaaa gaatgagaca aaagtgtttc ttacttttgt cccacgctct
    22141 tattcatgct cagagcgcca atagatacaa aatcatagcc aaagtttcac gaaccctagc
    22201 tgtcaaagaa tcgatatttt attttaatat aggctatttt aaaataaatg gtttaaggta
    22261 ttcctctaca aaagccactc tatcaattat catgggttta aaataagaag tgatggagat
    22321 aaccagattg ttcttagcgt caatataaag aacatttcca ctattaccaa ttgctgaata
    22381 tatttcgttc tcagaatcta atacccacca taaatagcca tatctcatat tttgtaagtc
    22441 ttctaaagca ctggttctag gtttgaacat ctcgtttatc cacatgggtg gtacgatttg
    22501 ttttccttta tacatccctt tatttaggca taatacacca atttttgcca gatcacttgc
    22561 cgataaacac aaaccatatc ctgccgtccc gatatttagt ggatcacaaa accagacatt
    22621 ggttttagga gacttatcca ttgtaaactg cttatgttct tcagctgttt cagcataata
    22681 attgacatgg tttggtatcc ctataggatc aaatagatac ttattggcaa aatcaacagt
    22741 tgttagctta cttgtctgat acaacaagcc agttaatatg tgtaaacaga cagtttgata
    22801 gttgaattcg ccggttatac cttttcttcc tcccaataaa tctagtgatg tttttgtcca
    22861 atcttcactt gaacagactt ttgtccatgg atcgccttta catttgtagg gtgctttcat
    22921 tgttagcaag tgcttgattg taatagcgta aatagttttc tccccgcgtt tcactttata
    22981 ctctggaaaa taatctaata ctttatcatc gatacttttg attaatcctt gctctaaagc
    23041 aatgccaatc agcaaagata caatactttt cgttgctgac atgatatgac aagtatcgtc
    23101 ttttttataa ccattccatt catctgtata aacttctacc ccatccttat atgcgacaat
    23161 ttgacatata tttggttgtc gaaaaccaat aaaatcatgc aactcttgtt tattcataag
    23221 atattacctc actttcttct ataagcataa tgtcattaaa aatcgatttc aattttttcg
    23281 attcttcacg taatacaggt attcttatgg actagacagc gctttgggaa gatggattct
    23341 ctgaaaaata taaaggttca ctttatgagt ggataaaaaa agcagcaaat acttcgactt
    23401 ctttaattta ccaacattag tgaaaatcga ttcatctgga gatttgtaat ttagagtc
//
LOCUS       DACDEG010000034        23102 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.036, whole genome shotgun sequence.
ACCESSION   DACDEG010000034 DACDEG010000000
VERSION     DACDEG010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.036"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IV142_001395"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IV142_001395"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP8485064.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IV142_001396"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IV142_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP8485065.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IV142_001397"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IV142_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP8485066.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IV142_001398"
     CDS             complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IV142_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP8485067.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IV142_001399"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IV142_001399"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP8485068.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IV142_001400"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IV142_001400"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP8485069.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="IV142_001401"
     CDS             complement(8053..8523)
                     /locus_tag="IV142_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP8485070.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="IV142_001402"
     CDS             complement(8569..8994)
                     /locus_tag="IV142_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP8485071.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="IV142_001403"
     CDS             complement(9061..9330)
                     /locus_tag="IV142_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP8485072.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="IV142_001404"
     CDS             complement(9341..10936)
                     /locus_tag="IV142_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8485073.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IV142_001405"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IV142_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP8485074.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="IV142_001406"
     CDS             complement(14489..15049)
                     /locus_tag="IV142_001406"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP8485075.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="IV142_001407"
     CDS             15402..16376
                     /locus_tag="IV142_001407"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP8485076.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="IV142_001408"
     CDS             16546..17946
                     /locus_tag="IV142_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP8485077.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVNLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="IV142_001409"
     CDS             complement(18024..18746)
                     /locus_tag="IV142_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8485078.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="IV142_001410"
     CDS             complement(18825..19331)
                     /locus_tag="IV142_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP8485079.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="IV142_001411"
     CDS             19454..21253
                     /locus_tag="IV142_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP8485080.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="IV142_001412"
     CDS             complement(21321..22895)
                     /locus_tag="IV142_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485081.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         6326 a         5015 c         4221 g         7540 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaataa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtca atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 ag
//
LOCUS       DACDEG010000035        22807 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.037, whole genome shotgun sequence.
ACCESSION   DACDEG010000035 DACDEG010000000
VERSION     DACDEG010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.037"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            92..502
                     /locus_tag="IV142_001413"
     CDS             92..502
                     /locus_tag="IV142_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP8485082.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            495..1196
                     /locus_tag="IV142_001414"
     CDS             495..1196
                     /locus_tag="IV142_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP8485083.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            1196..2782
                     /locus_tag="IV142_001415"
     CDS             1196..2782
                     /locus_tag="IV142_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8485084.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            2801..3022
                     /locus_tag="IV142_001416"
     CDS             2801..3022
                     /locus_tag="IV142_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP8485085.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            3019..3780
                     /locus_tag="IV142_001417"
     CDS             3019..3780
                     /locus_tag="IV142_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP8485086.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            4160..4669
                     /locus_tag="IV142_001418"
     CDS             4160..4669
                     /locus_tag="IV142_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP8485087.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(4744..5283)
                     /locus_tag="IV142_001419"
     CDS             complement(4744..5283)
                     /locus_tag="IV142_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP8485088.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(5423..6034)
                     /gene="rpsD"
                     /locus_tag="IV142_001420"
     CDS             complement(5423..6034)
                     /gene="rpsD"
                     /locus_tag="IV142_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP8485089.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            6350..7147
                     /locus_tag="IV142_001421"
     CDS             6350..7147
                     /locus_tag="IV142_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP8485090.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            7175..7810
                     /locus_tag="IV142_001422"
     CDS             7175..7810
                     /locus_tag="IV142_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP8485091.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            7830..8603
                     /locus_tag="IV142_001423"
     CDS             7830..8603
                     /locus_tag="IV142_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8485092.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            9011..9808
                     /locus_tag="IV142_001424"
     CDS             9011..9808
                     /locus_tag="IV142_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP8485093.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            9786..10199
                     /locus_tag="IV142_001425"
     CDS             9786..10199
                     /locus_tag="IV142_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485094.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            10183..12828
                     /gene="mutS"
                     /locus_tag="IV142_001426"
     CDS             10183..12828
                     /gene="mutS"
                     /locus_tag="IV142_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP8485095.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            12846..14954
                     /gene="mutL"
                     /locus_tag="IV142_001427"
     CDS             12846..14954
                     /gene="mutL"
                     /locus_tag="IV142_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP8485096.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            14976..15536
                     /locus_tag="IV142_001428"
     CDS             14976..15536
                     /locus_tag="IV142_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP8485097.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(15621..17609)
                     /gene="treP"
                     /locus_tag="IV142_001429"
     CDS             complement(15621..17609)
                     /gene="treP"
                     /locus_tag="IV142_001429"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP8485098.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            17832..18545
                     /gene="treR"
                     /locus_tag="IV142_001430"
     CDS             17832..18545
                     /gene="treR"
                     /locus_tag="IV142_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP8485099.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            18622..19068
                     /gene="msrB"
                     /locus_tag="IV142_001431"
     CDS             18622..19068
                     /gene="msrB"
                     /locus_tag="IV142_001431"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP8485100.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            19238..19840
                     /gene="ruvA"
                     /locus_tag="IV142_001432"
     CDS             19238..19840
                     /gene="ruvA"
                     /locus_tag="IV142_001432"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP8485101.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            19853..20854
                     /gene="ruvB"
                     /locus_tag="IV142_001433"
     CDS             19853..20854
                     /gene="ruvB"
                     /locus_tag="IV142_001433"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP8485102.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            20883..21461
                     /locus_tag="IV142_001434"
     CDS             20883..21461
                     /locus_tag="IV142_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP8485103.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            21513..21995
                     /locus_tag="IV142_001435"
     CDS             21513..21995
                     /locus_tag="IV142_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP8485104.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            22112..22486
                     /locus_tag="IV142_001436"
     CDS             22112..22486
                     /locus_tag="IV142_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485105.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7422 a         4075 c         4933 g         6377 t
ORIGIN      
        1 tcaaaaattg tatcttagaa acagcatgtt ttgaaacttt cagaaaaggt ttgttagtaa
       61 aatgaaagct atacgtaagg agtgttgttt gatgaaattt tttcaagagt ttttcattat
      121 tattttcttt tctgtattag gagaaggcat acggattttt gctcatttac ctattcctgg
      181 aagtatctta ggtattcttt ttttattttt agcatttgaa atgcatcttg ttgatcctga
      241 aaaaatcgga gcaactggtg attttttatt gaataatcta gccattttat ttgttcctgc
      301 tggtgtaggt ttattggaat attttgatga tattgcttcg atatggcctg ttttactggg
      361 agcagtcgtc gtgtgttcca tcgtcacgat ggttgcagcg ggaaaaacgg cagaaggtgt
      421 agaagctttg ctgggctatg tccgtaagaa aaaagcagaa agggtatctg taaacgaaat
      481 ggaggagaat caaattgact gagttttttg tgaatccatt atttggctta agtttgactt
      541 taggagtgta cgtattatct gacaaatttt tgcggaaata taaactgcct gttttgaatc
      601 ctctcgtatt atcgattgtt gttattatct tatttttgca tttttctggg atcagctata
      661 aagactataa tatcgggggc aattttttga ctatgctgat tactcctgcg acagtggcac
      721 ttgccatccc tctttataaa aacttccgtt tactgaaaga gaatttttat cctgtaatgg
      781 ctgcgatttt agcaggaata gtcgcaaatt gcttggttag tgtagggttc ggttacttct
      841 tctctcttca taaagagatg gtgatctcat tactacctaa atctgtaacg acggctattt
      901 ctgtcgattt atctcatacg atgggcgggg tgaatgccgt gacacttgct attgttgtct
      961 ctacaggtat ttttggttcg ctgatcgcca ctcatatttt ccgcctattc aagatcgaaa
     1021 gtccagttgc tcgcggtgtg gcgttaggaa gtacttctca tgctattggt acagcaaaag
     1081 caatcgagat tggtgaaata gaaggaatca tttccggact tgcgatatgc gtcaacggaa
     1141 tattgaccgt tttattgcta ccgttattct ttcagccttt tgctggcttg ttttgatgcg
     1201 aaaaccacta ttttatgcta caataaccaa aacgttcgag cataaaaaag gagggaaaga
     1261 cgtgtatttg tcttccgagt atcttaaaca atttgataaa gatctatatt ataagattct
     1321 cctcctagaa agttttgagg atcaagcttg gcataccgct gcacaattag cgcaagttgt
     1381 tcagctggat gcccgcagcg tgtcgaaata cctgaacgag ctgtctaaaa actatcagca
     1441 gtttagcggt aagacacacc ctttgtttac aaaaaatcac cgttctggtt ataactttta
     1501 tgatacgctc gattcaattg aacatgagcg ttttttgatc tatctcgtgc aaagtacgct
     1561 gaaattccag ttgctgcacg acattttttt tgaagaattc catacaatgt accaatttgc
     1621 gcaaaagcat tacattagtg aatctactgc tcatcgaaaa atcaatgaat ggaaacagca
     1681 actgcaaact tacggaattc ggctgcaacg aggtacatat atcgctcaag gcgaagaaga
     1741 aatcattcga ctttacttgc atatgacatt ctggcagttg tttcgaggga aaatctggcc
     1801 atttgaaacg atttctcaaa tggacgtaaa gaatatggca gaacatatca tggctttttt
     1861 taatgttcgt ctgaacgaga tcaaaaaaag acgattggaa tatatgctag gtgccttttt
     1921 tttaagaaaa tcacagaagc attatgttgt attgaatgag aaaaaaagac ggctgatttc
     1981 tgacaatctg ttgttccagc gattttgcca ggtcatggag ccggtatttc ctaattattt
     2041 tcaagtagaa gacgagttag gcgcattatt tcttgtactg atgactcgtg aagaatatta
     2101 tagtgatcct aagatacgga agaaaatttt tgattttcat caacaagcaa aaacaccgcc
     2161 ctttacagcc ctttcagaag caaaagccgc tctttctctt tatcaggaag agcaggggct
     2221 gccagcagaa aatctaacct tcgaggcaga aaactattta ttcagcagtc attttttcgc
     2281 ctatcttttt cctaatgcga aagaaacgat tgatggcaat agtagtgatt ttattaatca
     2341 tttagttatt gaaaacaagg aattgaaaca atggctcgta catttttttg aaagtagaca
     2401 caaacatccc aatcatctgg catttaaaaa tcatgcgttt ttgatggggc ggtatctgac
     2461 tgtgtttaaa acattaggag cctttactcc acagcttcca aagatcacga tccttttaat
     2521 gacagatttc ccattatttg aagagcaact gttagaggag ggattacgga catttttccg
     2581 taatgagtat cagctgattt ttctgccaac agattatcgg gggagagaag ttgatcttct
     2641 gatttcgaca agtaaagtcc accgcaaacc atgggcggat cttgactatt ttattgtgac
     2701 tgaagaacta aaattaattg attacatcca actatctcaa aaatttgaaa tgattcaaaa
     2761 acaaaaacta ttaaaacaat aacaaagaag gagaaaaaag atgacatttg aagaaatttt
     2821 accagaattg aaaaaaggtg caaagattat tcgtaaagga tggagtggat ttgaacttta
     2881 tgttgttctc gtatccgatg atgaatttga tggctgtccg gtgacgccgt acttattgat
     2941 caaaacaagt gacgaaggtt tttccagctt cgctcctaca gtttgtgata ttttagcaga
     3001 tgattgggcg atcgtagaat gaaaaaccag tatccagatc tagctaaaca ggttgtattt
     3061 atcacaggag ctgcttctgg aattggtgct gcgcaagccc gagcgtttct tgaacaagat
     3121 gcttttgtat ttggattaga tcaaaagttc ggtaaaatgg aagaaatttc tttgcgattt
     3181 cctgaaactt ttgcctacgc actaggtgat gttcgacaaa tgaaagaact ccaacaagcg
     3241 gtatcgaaat gccaaaatca ttttggtgaa gttacgattt tactgaatac agcggggata
     3301 ttggatgcct acaaaccctt gatggaaact gacgaatcgc tgtgggatct gatttatgag
     3361 acgaatgtga aaagcatgta tcagctgaca aaaatcatcc ttcctgatat gatccgccaa
     3421 aaaagcggaa cgatcatcaa tatggcttcg attgctggct tgattgctgg tggaggtggg
     3481 atcgcttata caagcgcgaa acatgcgatt gtcggattta ccaaacaact cgctctggat
     3541 tacgcaggac agggtatccg agtaaaagga attgctccag gagccatcca aacaccaatg
     3601 aatgcggctg attttgaagg gaaaggtgag atggcagagt gggtggcaaa tgaaacacca
     3661 gtcaagcgat gggcacagcc ggaagaagta gctgagctga cgttatttct tgcctctcct
     3721 caagcaagct acatacaagg aacgatcatc ccaatcgacg gcggatggtt gttaaaataa
     3781 ccttgtgttt ttttcaagct tctgtatgat aaaactaaag agagaaacaa cggttgatga
     3841 taaaaaaagt cgatgactgt tcccgacatt cgatgccgaa cagccagttc aaaaaattct
     3901 ttttaataat cgttaatatt cgaaactaag cggcttttca cgacctcttg atataaacga
     3961 tattcaaaag cttgcaactg cggtaacaag tccagccaaa tgaaaaatat aaggagctat
     4021 ttttatttga ccattcttat tacttgttgc agggcagctt tttcactacc ttctgcgaca
     4081 cttcagttgg aattttcttc ccatcctttg gtcgcggtca ttaccaagat aaaattatct
     4141 tgcaaaggag aaatagaaca tggaaaaaaa taatacccgt tcagccaaac gctggacaac
     4201 gacagatgtg actcggatgg cgctagtcac tggtttatat gtggcagtga cgctggtttt
     4261 gtctgtcttt agctttggag tgattcagat tcgtctctcg gagatgttca actatctgtc
     4321 cttgtataac aaaagataca tcgtcgcggt aacagcggga gtagctttgg ctaatattgc
     4381 ttctcctctt ggactgatag atgttgcagt aggaagcgtc agcacgctgc ttgtattgct
     4441 tatcaactat aaaatcacta gtcgcatcaa aaacatgaaa atcaaaatgg ccgtgacagc
     4501 atgcgtattt gctttttcca tgtttacagt agctggtcag ctaacgattt tatatcaact
     4561 accttttttc ttgaattggt gggtgattgc tttaggagaa ttggcatcaa tgattgtcgg
     4621 tgggataatt atttattgga tcggtaaaaa agtggatttg tcaaaataaa gatctgttta
     4681 atttaaaata gggcagcatg gaagagactg aatgttgagt cttcttccat gctgcttttt
     4741 tgattaattt gtatgaagaa atcgatttag ccgttttttc atcaagatac cgatataagc
     4801 tgctccagtt gccttgattg cttccggcag taaaaacggc agtagtccac tggcaaatgc
     4861 agttgtaaaa gtaagatcac cacttatttt cagccagatc gttccgcaga tcaatgcagc
     4921 aagagagccc attagattgg caaagattgc atataagtaa tgagcatccg ttctttcgat
     4981 catccaacca gttatccacg tttgtaagag aaatccaatc aaatatccgc cagttggccc
     5041 aaataaaaca gctatacctc cagtcatccc tgaaaataca ggaagaccaa tcaagccaag
     5101 taaaagataa actccaacag atatcaatcc tagttttctt ccgagaaaag ttgctgttaa
     5161 tccaatagca aatgtttgcc ctgtcaaagg aaccaaacca aaaggaatgg tcatctgaga
     5221 aaggacagaa ataatcgcgg caaattccgc agcaagtatc aattctttca atgttcggtt
     5281 cataacgccc tcttttcgtt aacttaatat ttttatttgg ttaacgaaat tgtaaaacaa
     5341 agtgattact ttgtaaatag aaaatataaa aaaagaccag ctatccaata gatagcggtc
     5401 tttttcaaga aagcttttga aattataagc tttggttgta gtattcaacg acaagagctt
     5461 cgtcgatttc tggatatagt tcgtcacgtt ctggtaaacg agtcaatgaa ccttccaatt
     5521 tttcagcgtc aaagcttacg aatgctgggc gtccaacagt tgcttctaca gcttctttta
     5581 ctgttacgat gttttgtgat ttttcacgaa ctgagatcac ttgacccact tctacgtggt
     5641 atgaagggat atcaacgcgt ttgccatcaa ctaaaatgtg accatggtta actaattgac
     5701 gtgcttgacg acgagtcgtt gcaagtccta aacggtaaac aacattgtct aaacgttgtt
     5761 ctagtaagat catgaagtta acaccatgtt taccttcttt gattttgcta gcttttacga
     5821 acaagttacg gaattgacgt tcgttcattc cgtacatgtg acgaagtttt tgtttttcag
     5881 ttaattgcat gccgtattct gattgtttgc cacggctatt aggtccatgt tgacctggtg
     5941 cgtaagggcg acgtgctaat tcttttcctg tgcctgacaa tgagatgcct aagcgacgag
     6001 aaattttcca tgatggtcct gtataacgag acattaaaat ttcctccaat gaatataagt
     6061 ttttggagta aaataatccg ttgaaaaatt cataatcata tagtttgttc ttcaatcttc
     6121 acctttgcag ccgcggttac acaatcgaac ccgagacggg gcaacaaact agggatgagt
     6181 tattattttt ctgctgcatt attttacaca aattacagta tatgatagat catccacgaa
     6241 tgtcaagata attagtacat aaaaacgctt tacaaaccta ggctgtcttt ataaaatgag
     6301 agggtatgtg caaatggaaa aaagcgaagg aagtttaagg agaaaatcaa tgaatccggt
     6361 atcaccatta tttgagaaaa tcgatgcttc aattgaaaag aaggcgaatc tttttgaaaa
     6421 cagcttttca cgttacgctg ttcgagcggt tctcgcctgt ttgtttttga ctttaggaac
     6481 ggctgtggca tttgcgatcg cgatgaaggg ggaagatatc gcgcatggtc taggaaagat
     6541 tttatacgca tttatgttca gctggggtct ggtcatgatt ctatacatga acgccgaatt
     6601 aggaacatcg aatatgcttt atatgaccgt gggtgttttt cggcagagac ttgctttaag
     6661 taaggcagcc aagattctat ttgcctgtat ttttttcaat atgattggcg gtatattatg
     6721 tggatttcta atttctatga ctacgccatt tcaagattta acgttggaca actttatgtt
     6781 agaatcaata gcagtaaaac tagcaaaacc ttcagcaacg attttggtgg aagggatgtt
     6841 tgcgaatatc gttgtcaata ccgctgtact gatcagcatg cggatgaaag aggatgtcgg
     6901 aaaagtgatc acagtcgtat tcatcatttt catttttgct tttttaggat atgaacacgt
     6961 gatagcgaat tttcctgcct ttacgcttac ttactttgct tctcacggac agatggatgg
     7021 aatgacagtc agcaatgtgt tacataattt gttttttgct ttggcaggaa attatattgg
     7081 tggcggttta gtaatgggac tgggatatgc ttggctgaat cagtcaaaat catcctatgt
     7141 ggactaaaaa gtcgttcatt aaaggggaag tgccatggaa gttttagttt ttccaaaaaa
     7201 tgaggaaaaa gcgttacaaa tggaaaagta catgaaaaat cttttttcct ttgctggagt
     7261 acctaagccg gaacgcgctc ttttacaaca accgttactg gatgagagca aaaatttatc
     7321 tatttctgaa gttttagctt ttgttcatta ttattatgaa caaatcgaaa gagagtatca
     7381 gtatgtagcg atcgatttag tccagaaaaa catcaaacgg cttacatttg aagacttagt
     7441 cgtattgttg ccactagttc agaaaaaaga atggtgggac agcatcgact cttggagaaa
     7501 agtatacagc acatggtgta aagcctaccc agagcagcgt gaagaagtct ttcaattgtt
     7561 tgaaaaaaag gaagattttt ggctacgtcg aatagcaatc acgttgcaat taggttataa
     7621 acagcaaaca gacacaggac tattgatccg agcaatagac gaagatcgtc aaacaaagga
     7681 attctttatc caaaaagcaa ttggttgggc attacgcgat tatagtaaga ccgatgccga
     7741 ttgggtaaaa agacagctga caacagattt gagtaagtta gctgttcgag aggcaagtaa
     7801 gtatttataa gaaagcgaga cgacaagaga tgagacgcaa attatttgct tttgatattg
     7861 atggaacgtt gttagattca aacaaacaag ctttagatag cacaagagaa gcattggaaa
     7921 aattacgcaa agccggtcat ctggtcacga ttgctacagg gcggagccga tttcatgcgc
     7981 aagagatcat tcgcgatctt gattttacga actacatttt atgtaatgga gcggccggtt
     8041 ttttagacca tgaacaagta tataagaatt tactagatcg cgaccagttg gatgcgttcg
     8101 ttcaagaagc gcatcatgag cagatcgata cagcttttgt cagcttagac aatatcaagc
     8161 gttcttcttc caatgatgta acggtgatgg aagaagcgat gcattctttc ggtgctgtct
     8221 tgcctgaatt agatcagttt tttgcagagg aacaagaaat ataccaagcg ttagcatttt
     8281 tcgatcaaac atatgatggg aagttctctt catatgataa aattcgattt gtccgctggc
     8341 atgagaacag tgtagatgtc gtacctaaaa atggttctaa agcagcgaca attctgcact
     8401 tagcagaaca agtaggcatc cctcatgaag acatcatcag ctttggcgat ggacaaaacg
     8461 accgtgaaat gttgcgcatg tcaggaattg ggatagctat ggggaatgct gtgccagaag
     8521 tacaagcaga agctaagatg gtcactgata caaacgatca ggatggaatt tggaaggcat
     8581 tgaaagagtt aagcgtgatt taaaagaggt tgtgaaaaag ctgttctgca tcaagtaata
     8641 agcccagcca aacaaaaata gctcctccta tttttcgttt ttctgggctt attaccgcag
     8701 atgcaagctt tcgaacaccg tttaaggaag aggtcgtgac agaagtggtc agtttcaaga
     8761 aataagaagg gatttacgaa aattgcttct caaatttttg taaaattacg gcttatttct
     8821 gaagaagtag atgaaacaac gtttattcgg gaaatgggac tgtgacaaga tgtttgtcat
     8881 agtcccattt ttttatttta tttttcaaaa ctaagaaata ccaggaaaga gttccttttg
     8941 tttgaccttt tctttcttat tgaaaacaac tataatatag agtgatgtta aaagagaaga
     9001 ggaatttagt gtgcgggtat tgtttattgg tgatgtagta ggttcaatgg gacgagagat
     9061 gattactgaa tatcttccta gattgaagaa gaagtaccgt ccgcaagtaa cgattgtaaa
     9121 tggtgagaat gccgcttctg gacgtggaat cacagaaaaa atctataaaa aatttttgca
     9181 agacggcgta gatgtcgtga cgatggggaa tcatacgtgg gacaaccgcg gaatctttga
     9241 gtttgtaaat gaagctaaaa agatggttcg tccggcaaat tttccggaag ggacgcctgg
     9301 acaagggatg gtctttgtca aagtgaacca agtcgaactg gcagtgatca acatgcaggc
     9361 tcgttctttt atggtggact tggatgatcc ttttcgtaaa atgaatgagc tggtagaaga
     9421 agccagaaaa cgtacgccga tcatttttgt agattttcat ggagaaacaa caagtgaaaa
     9481 acaagcaatg ggctggtttt tagatgggaa agtttcagca gtggtcggta cacatacaca
     9541 cgttcagaca aatgatgcgc gaattttgcc aagaggaaca gcgtatctga cagacgttgg
     9601 gatgacaggt ccctatgatg gtattttggg tatgcgcaga gaacctgtaa tcgaaaagtt
     9661 tctgactgca ttaccaaaac gttttgaagt tgtggaacaa ggccgatcga ttttaagcgg
     9721 ctgtatcttg gaattggatg atacgacagg tcatgcaaaa gaaatccagc tgattcagat
     9781 caacgatgat cgaccgttca tggagtagaa agagaggcag ttttttgcag caggagccgg
     9841 aaattcaaaa agaagtaagg aaactgacaa aacttttaag agagaatgaa acgatcattc
     9901 gctacaaaga acttgaagaa aagatccagc aaaaccagta tttagctgag ttgagagaaa
     9961 aaatcaaaca agcgcaaaaa gatgctgttc attttgccca ctatgacaaa ccagcagcag
    10021 aaaaagaagc aatcaaacaa gcggatcaat tcatgcaaga atttgatcag catccgcttg
    10081 ttgtagctta tagaaaacag ctgttggaag cagatgatct gcttcatcat ttaacaacta
    10141 tgatacaaga agaaatcaat ggacaaatag aggaggaaaa acatgcctca aaaaactaaa
    10201 aatactccta tgatggaaca atatctaagc atcaaggccc aataccaaga tgcttttttg
    10261 ttctatcgtt taggagactt ttatgaacta ttttacgaag atgcgatcaa agcgtcacag
    10321 cttttggaat taacgttgac gagcagaaat cgaaatgcag aagaacctat tcctatgtgc
    10381 ggtgtacctc accatgcagc acaaggatat atcgatgcct tgattgaaaa aggctataaa
    10441 gtagcgattt gcgagcaggt agaagatcca aagacgacta aagggatggt caagcgcgaa
    10501 gtgatccagc tagtcactcc tggtacagtc atggacagca aaggattgtc ggcaaaagac
    10561 aacaattttt taacagctgt agttagtcaa ggaaatgaat ttggctttgc ttatgccgat
    10621 ttaagtacgg gagaattgaa gacggctcgg ttgcatgatg aagaagctgt tttaaatgaa
    10681 gcctctgcat tgcagacaaa agaactcgta ttaggcagtg agatcactga tacccttcgt
    10741 gaacagctaa gcgggcgttt agggctagtc ttttctcaac aaaatgagat ggaagaaaac
    10801 gcggaattta gctttttgac aagcgaacta gtcgatccgc tggaaaaaga agcaaccggc
    10861 aaactattga cttatttatc ggtgacacaa aagcggagtt tagctcatat ccaaaaagca
    10921 gtagaatacc aaccagatca ttttttgaaa atggattatt attcaaaatt caacctagaa
    10981 ttaagccaat ctattcggac tgggaaaaag cacggcactc ttctatggtt gttagatgaa
    11041 acaaaaacag caatgggtgg ccgtttacta aaacaatggc tagaccgtcc gcttatccag
    11101 gcaaaacaaa ttaaagcaag acaagaaatg gtcgcttctt tgctagacag ctattttgaa
    11161 cgtatcgatt tgcagtcagc attaacgaaa gtctatgatc tggaacgcct agcaggtcga
    11221 gtagctttcg gtaatgtcaa tgggcgagac ttgatccagc tgaaaacttc tttagaacaa
    11281 gtgccaatga tcagagggtt gattgaagga atcgatcaag gtcaatggca aagtctgctt
    11341 tcagaaatgc agccgatgga ccatctcgtc caattgatcg accaagctat ccaagatgat
    11401 ccgccgttac aaataacgga aggaaacatc atcaaagatg gattcaatga gcagctcgat
    11461 atgtaccgtt ctgctatgag aaacggaaag aaatggttgg cagaacttga agcaaaagag
    11521 cgtcaagaaa caggaatcaa gaacttaaaa atcggattta accgcgtttt cggttattat
    11581 atagaaatta caaaagcaaa tcttggaaat gcagaactgg agaagtatga acgaaaacaa
    11641 actttagcaa atgcagagcg tttcatcaca ccagaactaa aagaattgga gacacaaatc
    11701 ctcgaagcag aagaaaaatc tgttgattta gaatatcagt tgtttttagc tgtgagagaa
    11761 gaagtcaaaa aggctatcca gccgctacaa gtacttgcaa aagcaatcag tgcagtagat
    11821 gtgctacaaa gttttgcaac gatcagcgaa cgctatcagt atgttcggcc agaacttgtc
    11881 agtgacaagc atcagcttct gatcaaagac ggacgacatc ctgtagtaga aaaagtatta
    11941 ggacatcagg aatatatccc taacagcgta gaaatggcag aagacgaaat gatcttgctg
    12001 attacaggac cgaatatgtc tggtaaaagt acgtatatgc gacagttggc attaactgtt
    12061 ctgatggcgc aaatgggctg tttcgttccg gcaaaacaag cagttttacc tatttttgat
    12121 cggattttta cccgaattgg tgcaagtgac gacttaattg ctggacaaag tacttttatg
    12181 gtagagatga tggaagccaa tcaagccctg cgttatgcta cgccaaacag tttgattttg
    12241 tttgacgaac tagggcgagg aacagcaacc tatgacggaa tggcgttggc ccaagcaatc
    12301 atcgaatata tccatcgtca tgtacaagca aaaacactct tttctactca ttatcatgaa
    12361 ctgacggtac ttgagaaaga acttccgcag cttaaaaatg tccatgttgg agccgttgaa
    12421 aaagacggag aagttgtctt cttgcataaa atgatggatg gacctgcgga taaaagctac
    12481 ggcatacatg tggcaaagat cgcaggattg ccttccaatc tactagaacg agcagcagat
    12541 atcctgcata cgctagaaaa tggcgataac ggacaccata ctgttccagc tgaagtagaa
    12601 gagaaaacta aaacagccaa acaggagcaa ataaaaccac ctgcagaaaa agtaaatcaa
    12661 gaccatatac aagaccgtgt aaaagaagaa aaccaacagc tttctttatt cagtgaatta
    12721 tccgaaaatg aaacagaagt cattgaatca ctgaagcaaa taaatttact agaaatgacg
    12781 ccaatggatg cactaaacac attatatgaa ttacaaaaac aactgtagga agaaggcgga
    12841 tatcgatggc caaaatccaa gaattatctg aacgtttagc taatcagatc gctgcgggag
    12901 aagtagtgga gcgaccagct tccgttgtga aagaattagt ggaaaatgca attgacgccg
    12961 gcagtactca gattgatatc ttagttgaag aagcgggact gcggacgatc caagtgattg
    13021 ataacggaga aggcattttg gcagacgacg tagagaatgc cttcaagcgg catgcgacaa
    13081 gtaagatcca taatcgcgac gatctttttc gcatacgaac gctgggcttt cgaggggaag
    13141 ctttgccaag tattgcctct gtttctgaaa tgactctgga aacggcgaat caggaagaaa
    13201 aacaaggcac ctatctgtcg ctgaaaggcg gagaagttat tgagcatcga ccagcaccac
    13261 tgcgtcaagg aacgaagatt accgtcagca atctgttttt caatacgcca gcccgcttga
    13321 aatacgtgaa aacactgcac acagaattag cgaacatcgg ggacatcgtc aatcgactgg
    13381 cattgagtca tccatcgatt gcttttcgtt tagtacatga tggaaacaaa atgctggcaa
    13441 ctgctggaaa tggcgatctg aaacaaacaa ttgcgggaat ctacggatta tctaccgcaa
    13501 aaaaaatgct gaaaatcgaa ggaaaagatc tagattttga gttatttggc tatgtttcat
    13561 tgcctgaggt gactcgtgcc agtaggaatt acttatcgac gatcatcaat ggacgtttta
    13621 tcaagaactt ttccttgaat aaagcaatcg ttgcaggtta tgggtcaaaa ttaatggtcg
    13681 gtcgtttccc tattgctgta ttggaaatca aaatggaccc gcttttagta gatgtaaatg
    13741 ttcatccgac gaagcaggaa gtccggctgt cgaaggaaaa agaactaaca gagctaatta
    13801 gtaacgccat tcaaaaagca ttgtctcaag aaaatctgat tccaagtgca gcggataatc
    13861 tacggtttaa gaaaaaagta cctgatcagc ctaaaacaga acaaatcgaa atcgatttgt
    13921 cctacgcttt tgaagaagaa aaacctaaaa agccaagtag tttgaatttt gatcgagaga
    13981 caggtacctt tttcgtagaa gaactaaaga acacatttgt tgacagaatt gttgataagt
    14041 ctgtggaaaa tgttgaaaac tattctggtt tttctgaaga aaacaccact tatccagaaa
    14101 aaacagcgaa aacggtggat aagtttggtg aaaatgttga taactttgta gaaaaaacag
    14161 tggaaaaatc tgaaaagaaa gaatttccca ttgtggataa catggagaaa acaaatgaag
    14221 aacaagcaga taaatccgta gaagaggatg aaaagattta cgaggaagaa ttgacactcc
    14281 atcctgaatt tgatttcact caaggaaacg acaaaacatt agaacaagcc atcaagaaat
    14341 tagaaacgga aaaaccagta caacgttttc ctcagttgga atattttgga cagatgcacg
    14401 gaacgtatct ttttgcccaa agtagcgatg ggttgtacat cattgatcag catgctgccc
    14461 aagagcgcat taagtatgaa tatttccggg aaaaaattgg ggaagtatct gatgatctgc
    14521 aagaattgct tgttccattt gtgttagact atccaaacag tgatgctttg aaattaaaag
    14581 agcaaaaaca cttgctagaa gatgcaggga tttatttaga agaatttggt caaaacagct
    14641 ttattgtacg ggcccatcct acttggtatc ccaatggaga agaagaatcg attattcggg
    14701 agatgatcga tatgctgctg acaactggaa gtgtgagtgt aaaaaaattc cgtgaagcaa
    14761 cagcaatcat gatgagttgc aaacgatcga ttaaagccaa ccattatttg aatgaagcac
    14821 aagcacgtgt gctgctgaag gatctggcac gatgcgaaaa tcctttcaat tgtccccatg
    14881 gccgtccagt attgattcac tttaccaatt cggatatgga aaggatgttc aaacgaatcc
    14941 aagacccgca ttaagtcaaa gagaagaggg aaaacatgaa gattgtatta gcatcccaat
    15001 caccaagaag aaaagagtta ttggctcata tcgtttcaga attcgagatt gaacctgctg
    15061 atatcgatga aacgccattt ccagacgaac agcccgtcga ttatgttcgt agaatggctg
    15121 aagaaaaagc acgaactgtc tgggaacaag atgaaaaaga agaccattta gtgattgcca
    15181 gtgacacgac tgttgttctc aatcaagaga tcatgggtaa accagaagat ctggctgagg
    15241 ctgaattcat gctgaaaaaa ctaagcgggg aaacacatac tgtatataca gctgttgttt
    15301 taaaaacaaa ggaacgagaa gaacggatac tagcagaagc acatgtcact ttttatcctt
    15361 tgacagacga agagatcaaa cgctacttgg agacggggga ttatgcggat aaggcagggg
    15421 catacggcat ccaaaatgct gcgagtgtat tcgtcaaaga aatttctgga gattattaca
    15481 gtatcgtagg atttccgatt ggtgctgtca atcaggcact gaaaacattc atttaaacaa
    15541 aaaagcggac tatctgattt tcgattgaaa atccatgata gcccgttttt tttattgaaa
    15601 agattactta tctactctga ctaagatttt acttcagctt taccgactgc ttcgcccggg
    15661 cgaacaagtc ctgattggtc aagagaaagt cctgcaactt tttcattatt ggtcaaagca
    15721 acaataatgt cagttttctt gccagcttgt tctatttctg gcaaatccat ttcagcaatc
    15781 agggtctctg gtgtgacaag gtatccttct ttcacgaata cattgaaagg aacacccttt
    15841 agttcaacag tatctaatcc catgtgaaca agtacttcca gcccctcatt agaaagaata
    15901 ccaatggcat gttttgtttc aaagacactc gtcactttac cgtttactgg tgcatagact
    15961 ttgccgtttg acggttcgac agcataaccg tcacccatca ttttttgtga gaaaactggg
    16021 tcatttactt cagttatttc cttgatggtc cctgcagcga ctgcgaataa tgtttctttt
    16081 gttgcaactg ccgtaccagt tgctgaaaca ggttcgaaag actgtggcgt tgctgttgcg
    16141 ccatttgctg tttgaagttg tacatcagca gcattttcag gaacagcagg atcaatctta
    16201 ttcaaaattc cctgacgtct aaagatgatc gtcaagccaa atggaatgat gacagcaatg
    16261 atcattgcga tgatgaaagg tacccatgtt tctgcttgga tagctagtat tcctggcaaa
    16321 ccgcctactc cgatcgcgtt agctcgaaca cccatcaagt tagcaaacat gccagctaat
    16381 ccagagccga ccattgcagc gacgaaagga tagacgtatt tcaaattgat tccgaacata
    16441 gcaggttcag ttacgcctaa gtaacaagaa atcattgctg gaatcgagat ttgttcttct
    16501 tttttattcc cacgatgtaa gaagatgatg gctaagactg cagagccttg ggcgatattt
    16561 gataaagcaa tcatcggcca taaattcgtt gatccgaaat ctgcaatcag ctgcatatcg
    16621 atggcattgg tcatatggtg gagaccagta atcactaaag gagcatacag gaatccgaat
    16681 acggcactaa acaaccagct gatagaagac gtcaatccag tatttacgat atttgagatc
    16741 cagcttccga ctgtccagcc gataggacct aaaataacgt gtgcagctaa aacagttggt
    16801 aacaatgaaa acaatggaac aaaaatcatc gagatcgact gagggatata tttgcggaag
    16861 aagatttcta gatacgctaa cataaaccct gccagcatgg ctggaataac ttgcgcttga
    16921 taaccaatca tctggacttg agcaaaacca aaatcccaaa atggaatatc tgctgcggct
    16981 gtggaagcta cactataagc atttaataat tgcggagaaa ctaacgtaat tcctaaaacg
    17041 attcctaata tttgtgtagt acccatcttt tttgcaatac tccaagtgat acctactgga
    17101 agaaaatgga aaattgcttc accgggtaac cacaaaaagg cattgacacc attccaaaac
    17161 ttcgagtgtt caacgattgt tccaccaaga ctttcaaact ggataccttc aagtacattt
    17221 ctgaatccaa ggatcaatcc accgacaatg attgcaggaa tcagtggtgt aaagatttct
    17281 gctaaaacgg caatagcacg ttgaacggga tgaaggtttt gttttgcagc tgcttttcct
    17341 tgttctttgg aaaccccttc aactccagat actgcaacga agtcgttata aaaagtagaa
    17401 acatcgtttc ctatgatgac ttggaactgt cctgcattcg tgaacatccc ttttacgcta
    17461 ggtatatctt cgatcgcttt ttcgtccgct tttcctggat cattcaggac aaaacgcata
    17521 cgagtcgcac aatgactaac tgcagcaata ttttcttttc cgccgatatc atgtaacagc
    17581 ttctcggcat ctgcttgata ttttgccata tcaataaacc cctttctctt aacttgtcta
    17641 tgcaagttgt ttcttcattt actatacttt ttttcggaga gaattgcaag cgttttatta
    17701 aaaagtacga gagaaaatga tatcgtttta aaaagcttaa aataagcttt tcttaaaaat
    17761 aggaacattg aaatacaaaa agagctgtag tatagtgaag aaatgatgga agaacgagtg
    17821 agggaaaaaa catgaataag ttcaatgaaa tttttttaga tttggaacag aaaattttag
    17881 cgaaagaata tcctccgcat tcgcttttac ccagtgagaa ccaattgatc aaaatttatg
    17941 gtgtttcacg agaaacaatt agaaaagcgc tgaatctttt gactacggaa ggttatattc
    18001 aaaaaaaaca aggaaaaggt tcgatcgtac tggatcggaa tcgcttcgac tttcctattt
    18061 ctggattgac tagctataaa gagctacagg agacccaacg tattccaagt gaaacgatca
    18121 tccatacgtt agaagaaaca gaagtgacaa aagcattgaa cgagatcact ggctgggaga
    18181 tcggtgcgcc tgtttggcat ttgatacgcg aaagaaaaat caatggcgaa gtagttatat
    18241 tagacaccga ttatctatta aaggaaatcg ttcctatttt aactccagca caagcacaag
    18301 gatcgatcta tgaatatttt gaaaatgagc tttctttgac gattgcttac gcgcaaaagg
    18361 agatcacagt agaagaagta acggaaatca tgaaaacagc gatggatctt cacgatgaca
    18421 cttatgcagt agtagtcaga agcctcgttc atctggaaga tacccgctgt ttcgagtatt
    18481 ctgagtcggt tcaccgatta gataaattcc gtttcgttga gtttgcccga cgccgaaaag
    18541 tgtaaatagt tcaaaagaaa gcaaattgtt ggcatctgtc cactttggcg ctatgatagg
    18601 atcacaaagg gggcattttt aatggataaa ccaataagac cagaaaaaga agaactgaaa
    18661 gaaaagttat cacccatggc ttatgcagtc acacaagaaa atgcaacaga acgaccattt
    18721 agcgggaaat atgatgattt ttacgaaaag gggatctatg tagatatcgt cagcggtgaa
    18781 ccgttattca gctctgcaga aaaatatgat gcaggttgtg gctggccatc attcagcaaa
    18841 ccaataacga aaagacaagt gcgcgaaaaa gccgattttt ctcatggcat gcatcgaata
    18901 gaagttcgaa gcaaagaagc agattctcat ctcggacatg tgtttaccga tggaccagta
    18961 gatcaaggtg gtctacgtta ctgcatcaat tctgcctcac tgaaattcat tccatatgaa
    19021 aagatggatg aattaggtta tggggaattc aaatcacttg tagaataatg gaaatacaca
    19081 aagagcggta caatacgggg atttactcga tcgtccgctc tttttttatt caagcgattg
    19141 ctatttgaaa gaaacgctcc ctagactcct tttttcacaa ggtgaaatgt gctacaataa
    19201 agggaacgta tgtgtgcaaa agaaagagga ctagaatatg tatgaatatc tgacaggtgt
    19261 ggttaccttt atcaacccct attacatagt agtagaaacc aatggtatcg gctaccagat
    19321 cgcactagga aatccttacc gctacagtag caaactaaat caaaaaataa aaatttatgt
    19381 ccatcaggta atacgtgaag atgcgcatct tctgtatgga tttgattcgc tagaagaaaa
    19441 gcaactgttt ttgcgattag taagtgtttc aggaatcggt ccgaaaagtg cccttgcaat
    19501 catggcaagc gaagaccact ccggtttgat tcaagcagta gaatccagtg atgtcactta
    19561 tttgacgaaa tttccaggag tagggaaaaa gacagcacaa caaatgatct tggatctaaa
    19621 aggaaaattc ggtgagctga gtatcgatac accttttagt ctatttgatg aagcaaacac
    19681 aaaagacgct acagctctat ctgaagcaat ggaagctctc tcggctcttg gttacagtga
    19741 tcgagaaatc aaacgtgtag aaaaacaatt aaaagaagta gataatcaga caacggatga
    19801 gtatctacgc caagcactca agctaatgat gaaaaaataa ggaggagacc aaatgacaga
    19861 tgaatacttg ttatcaccag aatctggcga aaatgaactg agtttggaaa aatctcttcg
    19921 accacagctg ctttctcaat atatcggaca gagaaaagtc aaacaagaac ttcagattta
    19981 catcgaagct gccagaaatc gggaagaacc tttagatcat gttctgttgt acgggcctcc
    20041 tggattagga aaaacgacga tggccatggt cattgctaat gagatgaccg taaatattcg
    20101 tacgacgagt ggccctgcta tcgaacgtcc gggtgactta gtagcaatct tgaatgaact
    20161 ggaagcaggg gatgtcctgt ttatcgatga gatccatcga ttacctagag tggtagaaga
    20221 gatgctttat tcagctatgg aagatttcta tgtagatatc atggtaggac aagggccaac
    20281 cgcacatccg gttcatttcc ctcttcctcc ctttactttg attggcgcga cgacacgggc
    20341 aggtatgctt tctgcacctt tacgagatcg atttggcatc atttctcata tggaatatta
    20401 tgacgaacag gatctgcagg agatcgtttt acgttcttcg gatatttttc aaacagagat
    20461 cattgaagaa ggggcattgg aaattgctcg ccgatcacga ggaacgccta gaatcgctaa
    20521 tcgcttgctc aaacgggttc gtgattttgc gcaagtccaa ggagacggga aaatcgatcg
    20581 tcctatagcg gatcaggcac ttactttgct acaggtcgat caggctggat tggattatgt
    20641 tgaccagaaa ttgttgcgaa caatgatcga gttatatggc ggcggaccag tagggttaag
    20701 tacgctttca gtaaacatag gagaagagcg tgagacggta gaagatatgt atgaaccgta
    20761 tctgatccaa aaaggatttt taaagcgtac acctagaggg cgaattgcta ctgcttatgc
    20821 ctatgaacat tttggttacg actatcatat atgatttttg cttttagatt ggaggaaaat
    20881 gaatgagtga tacaacacgt acgaaacaat tgatcaaaaa cgtattgatt gatttgtgcc
    20941 aaacgcaatc ttttcgaaaa atcagcgtcc aaaaaatcgc aaacgaagca aaaatcaatc
    21001 gacaaacatt ttattatcat tttacagata aatacgattt gcttcgttgg gtctattgtg
    21061 aggatgcatt gcattattta gatccacaat gtctttcact tgataattgg gaagagcaag
    21121 cattgaaaat gctgaaagca attgctgaga agaaagaatt ttatgcaaat actgtgcttt
    21181 ctgacccaga aattttacaa aaagagtttt ctcaactgat ccaaaaatca tttatcaaga
    21241 tttttgaaca aatggatgaa gaaagacaac tttctgcaag agacaaacta ttttatgctc
    21301 gttttttttc ttacggttgc agtggtgtat tagtcaattg gatcacgaat gaatatgaag
    21361 aaacaccgat ggaaatcgct atgcagcttt tccgtttagc caaggatacg gaattatatt
    21421 cttatcgtct ttatgcaaaa gaagagttgg aagaacagta atagaaaaat tttttgttgg
    21481 aaaagcaata atcgctaaaa ggagatgggg aaatgacaaa aattttattt gtttgtttgg
    21541 gaaatatttg ccgttcaccg atggcagaag gcttgctgag aaaacgaata gcattagaag
    21601 gaagggaagc agaatttttt gtggattctg ctgctacaag cacgtatgaa gttggaaaga
    21661 caccgcaccc tggaacgaaa aaaatattaa accaagaaaa agtagatatg acgaatatgg
    21721 ttgctcggca aatcacaccc cacgattttg aaacttttga ttggatcatt ggcatggatc
    21781 aagaaaatgt agaagaactg aaacgaagag caccacgctc agcgcagggg aaaatccatt
    21841 tgtttttaag tagcattcct ggaaaagaaa aagaaaatgt tcccgaccca tactatacca
    21901 ataactttga tgagacatat cacttgatca cagaaggcct gaatcattgg tggaatattt
    21961 ggattgatga aaataaaata acaaacaatt tgtaaagtta acgctttctt tttaaggcga
    22021 tgttagagca tttccttgtt tttttgtaaa atgttaggta aaataaaatt aattaagtaa
    22081 cactttctta gctaaaggag gagtgcattc gatgaacgaa gagacagtat cgccaatgaa
    22141 gaagcttacg aagttagtta ctcatacttc gtatcaactt tttgattctt ttgtaaatga
    22201 cactgaagaa catgctaaga atgtacgatt gcaaggaaag tttattcttg agctgcaaaa
    22261 agctgctact gaacatagtc tagttatctt acaagtaaga gcaaatcgtt tgagtagcaa
    22321 gtatgaaacg atctcaggct gggtggcgac aaaaacggtc gaaaaagacc gagtgatgat
    22381 ccgcctacaa aacgaagagc agcagatccg gatggttcct gtagaacaaa ttgaaaaaat
    22441 aacaatcctt tcaccagcag gggaacagga acggttatca aaataattta taccagatag
    22501 aaaagcacgt atgagcttgt accatcaatc attactaatc aaaaataacc gaatgcttta
    22561 agctgatatc agtaaaacgg aacgttttag gatgagtatc atagcatgac agcattcggt
    22621 tgattagttg aatcgattgg atggtacaag tttttttgat aaaaaaaggt cctgctagcc
    22681 ggggagctag caggataagg agttaaaaat gaaaaagtgt tgttagggtt gtttgttggt
    22741 atgcttatat aatagtcatt agttgtgaat attttgtgac gaaagttttc gaaagttgtt
    22801 aagtttt
//
LOCUS       DACDEG010000036        22804 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.038, whole genome shotgun sequence.
ACCESSION   DACDEG010000036 DACDEG010000000
VERSION     DACDEG010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.038"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..1504
                     /locus_tag="IV142_001437"
                     /pseudo
     CDS             1..1504
                     /locus_tag="IV142_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            complement(1612..2694)
                     /gene="uxuA"
                     /locus_tag="IV142_001438"
     CDS             complement(1612..2694)
                     /gene="uxuA"
                     /locus_tag="IV142_001438"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8485106.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(2685..4331)
                     /locus_tag="IV142_001439"
     CDS             complement(2685..4331)
                     /locus_tag="IV142_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP8485107.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4515..5177
                     /locus_tag="IV142_001440"
     CDS             4515..5177
                     /locus_tag="IV142_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8485108.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            5321..5884
                     /gene="ahpC"
                     /locus_tag="IV142_001441"
     CDS             5321..5884
                     /gene="ahpC"
                     /locus_tag="IV142_001441"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP8485109.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            5894..7423
                     /gene="ahpF"
                     /locus_tag="IV142_001442"
     CDS             5894..7423
                     /gene="ahpF"
                     /locus_tag="IV142_001442"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP8485110.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            7580..7930
                     /locus_tag="IV142_001443"
     CDS             7580..7930
                     /locus_tag="IV142_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP8485111.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            8029..8559
                     /locus_tag="IV142_001444"
     CDS             8029..8559
                     /locus_tag="IV142_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485112.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            8713..9864
                     /locus_tag="IV142_001445"
     CDS             8713..9864
                     /locus_tag="IV142_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP8485113.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(9973..12012)
                     /locus_tag="IV142_001446"
     CDS             complement(9973..12012)
                     /locus_tag="IV142_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP8485114.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(12070..12627)
                     /locus_tag="IV142_001447"
                     /pseudo
     CDS             complement(12070..12627)
                     /locus_tag="IV142_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            complement(13166..13372)
                     /locus_tag="IV142_001448"
     CDS             complement(13166..13372)
                     /locus_tag="IV142_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485115.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(13388..14002)
                     /locus_tag="IV142_001449"
     CDS             complement(13388..14002)
                     /locus_tag="IV142_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP8485116.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(14149..14832)
                     /locus_tag="IV142_001450"
     CDS             complement(14149..14832)
                     /locus_tag="IV142_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP8485117.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(14941..15399)
                     /locus_tag="IV142_001451"
     CDS             complement(14941..15399)
                     /locus_tag="IV142_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP8485118.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(15483..17246)
                     /locus_tag="IV142_001452"
     CDS             complement(15483..17246)
                     /locus_tag="IV142_001452"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP8485119.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(17467..18927)
                     /locus_tag="IV142_001453"
                     /pseudo
     CDS             complement(17467..18927)
                     /locus_tag="IV142_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19135..20061)
                     /gene="glsA"
                     /locus_tag="IV142_001454"
     CDS             complement(19135..20061)
                     /gene="glsA"
                     /locus_tag="IV142_001454"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP8485120.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(20084..21511)
                     /locus_tag="IV142_001455"
     CDS             complement(20084..21511)
                     /locus_tag="IV142_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8485121.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(21542..22756)
                     /locus_tag="IV142_001456"
     CDS             complement(21542..22756)
                     /locus_tag="IV142_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP8485122.1"
                     /translation="MNTLPINTGGTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         6757 a         4459 c         4007 g         7581 t
ORIGIN      
        1 tacaaaatta gatatttttc ttttcctatc tgaacatcgt ttctttatta ctacacaata
       61 tttagcagat catttccata tgtctgaatc aaatttttta ctatacatca aagaattaga
      121 acaagacttt gagcgtctca atctaacaga actgcatatt gataagcaaa aacctttttt
      181 aaagttgaat tttgaaggaa ttgatccggc ttactgctat tatcgtctat ttggaagata
      241 ctgcaacgaa tcagtcagtt atcagatctt gacttctttg ttcagctgtc agacaaattc
      301 gattatcagt ttcagtcagc aaacaaacta tagtgcctct tatttgtata cgaaaatgaa
      361 aaagattaat gcatttttag catagtacgg tttgtctatc tccttttcta ataatggcag
      421 aaagtctttt tctggaaaag aggtacaaat tcaatatgca gcattagata tttattggaa
      481 catcttttca agtactgcta cagaatttta tgatgaagat ccgcaagtag tggcttttat
      541 gatgaatact ttttttaaaa aagaagtact tgaccgacta aatagcggta tgatagataa
      601 attatatttt ctattaaaaa tatgtaaaga gaattttcct gcaacttctg tgaaagacgt
      661 acaaaaagaa ttcacagagt atgaatatat tgactttttt attgatctct ctgtagatat
      721 tttgcgacct aatctgccaa tttgtcgcga acaacgcatc ttagtgaata ttttagctcg
      781 actttctatc acgaaaatcg aatcggaaga aacaagcttg gagcaataca aacttttaaa
      841 aaaagcaaac gttcctcatg ttctttattc aagagaaatg gtggagtccc tttgtactgt
      901 atttgatttg tttatacctg aaaaagaaaa gattctttac tcattggcat tcgcacgaaa
      961 taaactttat aatggatttc ttaacttcaa tcaaccaaat acgccccttc ctactttcat
     1021 gctttatcaa gaacatagcg tattgaaaga agtccaaacc gccattgaac aattttatat
     1081 ggatttcaga gaacaaaatc aacacttgtt accagttatt ttagaaaagg aaaatgtgca
     1141 atggatggtt gaagacctag tacatttgta tgatcgctac aaaaaacagc cgcgaattgt
     1201 gattggtgtg aactatacgc gtgattttta tatcagtaaa gatctgatcg ttaagataga
     1261 acaaattttt tcaaatgaat cgatcaaaat acaaaatgat tttatggaga actgcaacat
     1321 cgttatcagc gactgccctt tgaaacaatt gccttctcat atcaaaaaaa tctatatttt
     1381 aaaaggaatc atcacaccag aagactggaa aaaagtgatt atgaggatct cagaatatat
     1441 ctttgaatta aaagaagaag cactcatgga tcaatcgaaa aaagaaaatc aagttacttc
     1501 ctagaccaaa taagatacat aaatccgaat gcttattaac aacctccgga tttatgtatc
     1561 aagaatttga acatccttct tatttacata ataaaattac agtttttttc actaattatc
     1621 tttacttttg tttccttttt cgattgcttc ccataatcca tttagataaa cagcacctaa
     1681 tgctcgatca tataagccgt atcctggtct tcctgtctca ttccagatca ttcttccatg
     1741 atcaggacgg atgtatccgt taaatccgtt gtcatacatt gtttttagta tgcggtacat
     1801 gtctaatgaa ccgtattcgg atttatgtgg tgcctcatag aaatctcgat tagctaaaaa
     1861 tttaatattt cgaacatgtg caaatgggat tcgatctctt tggcaaaatt ctgctaaaat
     1921 cccatataca tcattttctg gatcttctgc aatactccct gtacaaagag tgattccgtt
     1981 ataagggctg tctactatgc gacacaacca atctaaatct tctcgattct ttacgattcg
     2041 agctaatcca aaaatcgagt atggcggatc atctggatgt acagccattt tgacccctac
     2101 ctcttcacat gttgggatga ttgattggag aaaataggca aaattttctc gaagtttttc
     2161 ttcgtctact gatgcatagg cttcaagcaa ggatttgata ggagctaagc gctcaggttc
     2221 ccatcccggc aatccaaatt cattgctgga atgttcgatg cgcgcgacga tttctcttgg
     2281 atcatctgga atatcatcag atataaaagc taacgtggat gagccgtctt ctaagcggta
     2341 gtcaaggtca gatttcaccc agtcaaatac aggcataaaa ttatagcaga tgacttctat
     2401 tccatattct gccaagtttc ggattgtctg cttatagttt tcgatatact gctcacggga
     2461 aggtaatcca attttaatgg catcatgcac attgacgctt tcaattactt ttagtgttaa
     2521 cccacgagac tcaatttctt cttttagttc tttgatcctc tccctaggcc atacctctcc
     2581 tactggtata tccatcaatg tgcctacaat ccctttgacc gcaggaatct gtctgatttc
     2641 atctaaggta atcttttctt cctgtgatcc gtaccatcta aaagtcattt ccatgataaa
     2701 tttcctccat gttcttgtaa gtaatcctgt aatgtttttc gtacggcatt tggtcctgct
     2761 accatcttga tgaacatttc ttctattttt ctgccaagct ctgcttggta aagatctaca
     2821 ccaaaaattt ctttatttga caaaatagga gccattttct catgaatatg tgatgtatct
     2881 tgcgtaccca gttgaatatc ggaaagctta tgctgaaggt ctgtcaatag tgggtctgga
     2941 ctcgggatga atggttttcc ttgatcatca atgccaataa ggtatcgaag ccaagcagcg
     3001 agagtcaatg gtatgaatac aagtgtctgc gggtctttaa cttctatttc catatatttt
     3061 ttaatcgtct caccataacg aatcgccacc ttttgagagg tatccgtggc gattcgctga
     3121 ggcatattag gaatatttgg attagaaaag cgcacagtaa ttacttcttc cagaaagatc
     3181 tttggatcaa tgatacctgg agactccaca actgggaggc cttcattgta cccgatttca
     3241 gaaactagca gcgataaatc tggatctttc atctcctcag ctatgctcct ataccccagt
     3301 aaacaaccca taatagctaa agacgtatgc aaaggattaa ggcaagtcgt caccttcatc
     3361 gtatctgctt tatctaccgt tgcacgatca gtcaagaata ccccagcttt ttcgaatttc
     3421 ggacgtccat tgggaaaatg atcttccatg attaaataat ggatgtcctc tgtattgaca
     3481 aatggagcaa tattatttcc ttttgcggtt tggaaaatcg acatatttga aaagccctct
     3541 tttgccaaca ttttgctaac tgacaaggaa ggattcgggg tgatacgatc aatcatcgtc
     3601 catggaaacg caacaatttc ttcattcgat aagtattgga taaatgtatt ttgtacataa
     3661 ccttcctttt gccattcttt tgcgatcgtt agcaaagaag tacgtaattg actaccgttt
     3721 tgtgaaaaat tatctgtaga gacaagtgca atcggccaag ctccagataa aaaacgttta
     3781 taaagtagca cagcaagaat actcattgta tgtgatggct tctttggacc attttttata
     3841 tcgactgcaa tcggtcctat atatttatta tttgtatctt tcaatcgata acctttttca
     3901 gtaatagcaa aagtaactaa ttgtaaacta ggattttcaa agtgtcgaat catcttatca
     3961 aagtgttcag aacaatctgg atgacaatac agactgtcag ctgtcgcagc taatatacgt
     4021 ttgacgaact ctccactgct tggcatgacc acttcaagaa tcaaatcatc aaaatcttgg
     4081 taagcccttt caattatttc ttcatcgaat gtttcacaaa caatgacacc agtggacaat
     4141 tcgcctagat ccgctaaacg ttgagcgatt tctgcatgaa atcctcgata aagatttccg
     4201 ccaccgaaat ggatccagac aggatttttt tgtccatttt ctcttaatga tgcacggtca
     4261 aaatgaggta tttctacttc tttttgagat aattcaggga taaagttcct ccagttgttt
     4321 aaatcaatca tttttcccct cctaaactaa catattagtt tttgtattca agtgaatttt
     4381 atgcaataaa aatcgctatg tcaaccaaaa ttttactatt tatacaaatt tactagttaa
     4441 tgtgacatac cagattgttt ttcagacctc ttctgttata ctacgttcat agaaatgatt
     4501 ttggaggaat aaagatgaaa agcagcttgc aatcacaagc ttatcaagcg attcgtaaac
     4561 aaattatcta tgctgactta gaacctggaa ggaaaatatc tgaaaaggca ctggaggaac
     4621 atcttgctat cggccggact cccattcgcg aagcattgat tcaactacgt cagcaagaac
     4681 tcgtgtatac gatacctcaa agtggtacgt atgtttccaa aattaatctt gtttctgcag
     4741 aaaatgctcg ctttgtccga gaacagttgg aagagcaaat catgattgaa tgttgttcca
     4801 aattggacac acaaacacga atagtgctag aaacgatttt agaagaacaa gaaaaagcag
     4861 tcaaaataaa tgacgaacgc gcttttttcc gtgcagataa tttatttcat gaagcttgtt
     4921 tcgaaattgc aggaagaaaa gaagtttgga actggttaga tgaccataat acccatttag
     4981 aacgtttccg ctggttacga gtgaccattc aggggcttcc atgggaaacg atcatggaac
     5041 aacattatga tttactcaag gctttagttg ataaaaaacc agaagaagct cgatttcaaa
     5101 cgggattgca cttacacatg atgctgaaag aacaggaaac tgttactcaa cgttatcccg
     5161 agtatttcca gcagtagcat tttcttgcga cctcttctat ttttataatt aaccttcgtt
     5221 tttgagtaat ctttattaaa taacaaaata tccattgact ttgcaactcc aactaaatta
     5281 caataattat aaataaataa ttattattgg aggttttact atgtcattga ttgggaaaaa
     5341 aatcgaagaa tttaatgcac atgcttatca tcaaggagaa tttttagaag tcagtgaaaa
     5401 agatcttatg gggaactgga gtattctttg cttctatcca gctgatttta cttttgtttg
     5461 tcctactgaa ttagaagact tacaagaaca atacagtaca ttgaaatctt taggtgttga
     5521 agtcttttct tgctctaccg atacccactt tactcacaaa gcatggcatg atacatcaga
     5581 tgctatcggt aaaatcgaat acgtgatgat cggtgatccg tctcatcaga tttctcgtat
     5641 ttttgatgtt ttggatgaag agcaaggatt agctcagcga ggaacattta ttattgatcc
     5701 agacggaatc gttcaagcta tggaaatcaa tgcagatgga atcggtcgtg acgctagtgc
     5761 actgattgat aagattcgcg ctgctcaata tgtacgtacc cacccaggtg aagtttgtcc
     5821 ggcaaaatgg aaagaaagtg gcgaaacatt gaaaccaagt tttgatcttg ttggaaagat
     5881 ttaggtggtg gatatgttag acaaagaaac taaacaaaat ctcgaacaat atttagcact
     5941 gattgaatct cctattgttt tttctgttag tttggataat agtgaaaata gtcaaaaact
     6001 agctgaattt acgaaggaaa tagcagaaat gtctcctaaa atctcttggg aaaaaagaga
     6061 ttcgatgcgc acacctagtt tttctatcaa tccagttggc aaagaaagtg gaatcgtttt
     6121 tgctggtatc cccctcggtc atgaattttc ctctttttta ctggctatgt tacaaattag
     6181 tggacgggca ccgaaaatca gtgaatcttt gagtcttaaa atcaaaaaaa taacacaaac
     6241 attgcatttt gacagttatg ttagcttgac ttgtcataat tgccccgacg ttgtacaggc
     6301 attgaatata ttagctgtat taaatccaaa tattactcat acgatgattg aaggtgggat
     6361 gttccaaaaa gaaatcgatg ataaaaaaat catggcagtg ccaactgtgt ttttaaatgg
     6421 agaagaattt agtagcggac gcatgacgat cgaacaaatt gttgaaaaaa tcactggccc
     6481 tttgattgaa gaagagttgt ttgaaaaaga accttatgat gtattagtga ttggcggtgg
     6541 accagcagct agtagtgcag ctatctatgc agcacgtaaa ggaatccgta caggacttgt
     6601 cgctgattct ttcggcggac aagtcgttga aacactaggg attgaaaata tgattggtac
     6661 ctcttacaca gaaggtccaa aattgatgca gcaagtagaa agtcatgtgc gtagttaccc
     6721 tatcgacatt atgatgaatc aacaggccgt ttccttaaat aaagagcaac attttattaa
     6781 tattggctta gctaacggta tatcacttaa agcaaaaacc gccatcatcg cgacaggtgc
     6841 gcactggcgt tctatcaatg taccaggcga aaaagagttt aaaaataaag gaattgctta
     6901 ttgcccacac tgtgacggcc ctttatttaa aggaaaagag attgctgtta tcggtggtgg
     6961 taattctggt gttgaagcag caatcgattt ggctgggtta gcaaaacatg tatatgttct
     7021 agaattctta ccagaattaa aagcagatca aatcttacag gataaactag tctctcttgc
     7081 gaatgtcact gttatcaaac atgcagcgac gaaagaaatc agcggtacga atcaggtaga
     7141 atctctctcc tatatcgatc gccgtacgat ggagatgcat acactagctg tttcaggagt
     7201 attcattcta gtcggcttac tccctaatac ggattggcta gatagaacga tcgaaatgaa
     7261 tccaagaaat gaaatagtga ccgagaaaaa tggcgcaacg aatatccccg gcgttttcgc
     7321 agctggtgat tgtacagata gtccatacaa acaaatcatt gtttcaatgg gttcaggagc
     7381 tactgcagct ttaggagcat tcgattatct gatgcgcact tgagctatga agattgtaaa
     7441 aagtcagaga gcggaaaaaa ggatggtgac aagaattttg tcacaatcct ttttgttttt
     7501 ttgaataatc gctttttctt ctctttttct tatatactat aagtagtaaa taatttgaaa
     7561 aaaagaaagg tgactgatta tggcgcaaaa tcgtattttt acgacaagtt ttgcaagcgt
     7621 ttatcctatg tatgtagcaa aagcggagag aaaagatcgt ataaaagagg aagtagatga
     7681 aatcatttgt tggctgacgg gttattccca aacagaacta gaatatcaac tacaaaaaca
     7741 agtgactttc gaacaatttt ttgcggaagc ccctgtcccc aatccttcac gttccttgat
     7801 tacaggtgtt gtttgtggtg tacgagtaga agacatcgag gataacttga tgaaagaaat
     7861 tcgttattta gataaaatga tcgatgagct agcaaaaggc aaaaaaatgg aaaagatcct
     7921 cagaagttga tttttccaga gatattcttc tccttcgctc attttgtaac atttaatttg
     7981 gtaaaatgac tagttcacgt taaaataagt caaaggaggt tttcttatat gtttgagacg
     8041 attaaacaaa atattcaaaa atacgcctta ttaagagctg ctgtttatat tatttctggg
     8101 attgcgattg tgctaaaccc ttctgctgtt tttcatttta tcggttatct gatcaccgcc
     8161 tatcttgttt tgcttgggtt gttgaatctt tttgaggctt accgttttaa aaggaaaaca
     8221 ggttcttgga gtatcggtct gttcagtggt ctttttttcc tgatcttagc tctgatcgtc
     8281 tatgcttttg cacctgcaat cgtttctatc ctgccgattc ttcttggatt agctgttatc
     8341 atcaacagct tgttccagtt gttcttcagc atgaatacaa aagctaaaag ttggagtgtt
     8401 tatagtagta ttctgttgtt gattggcgga ttcgtcttat tgttcaatcc attcaaaagt
     8461 ttgatgcttc tattccaaat attcggcttt attttgattt tcatgggcat ccaggagatt
     8521 atcaatttca tcagaataag aaaaatgact atacgttaat catttttagt tattaaaata
     8581 gaccgtataa aaaccaacaa tccaaaaaaa tcttttggat tgttggtttt tttgtgagta
     8641 tattttccta ttttttcaat aatgtatgtt atgctaagtt cgggctatat tttatacaaa
     8701 ggaatgatga gaatggaatt tattggtatt ttatgcctga tattgatttc aacaacgatt
     8761 ggctcacata tctcgcggag attaggtatc ccagcagtga ttggacaatt acttgttggt
     8821 gttttgctag gccaagctgg attgaattgg gtcaaacctg atattctagt ccatgacttt
     8881 tcggagattg gtgtgatctt attaatgttt ttagctggtc tggaaagtga tttatctctt
     8941 ttgaaaaaat atttccgacc tggtatgttc gttgctttgc ttgggatcat cttcccggtt
     9001 gtttttggct ggttgacagg caaagcattc caagtttcgg gaaatgaagc gattttcttt
     9061 gggattattc ttgcggcaac ttctgtcagt atttcagtag aagtattaaa agaactaaac
     9121 gtagtgaata caaaagaagg atcaacaatt ttgggtgctt ctgtggtaga tgacatttta
     9181 gtcgttttag ttctaagttt cagcctatct tttctaacgg gggaagcttc tagcaatgct
     9241 tctttaccgc ttgttttgat tgaacagcta atttattttg tttttatttt cttattagtc
     9301 aaatggattg cgcctttcct actttcattt gcagaaaaag tgtacgcaaa ttcttctatt
     9361 atcatcgtat cgctagttat ttgtttagga atgtcttacg tagcggatct tgttgggctt
     9421 agctctgtca ttggtgcttt ttttgctggg atagctgtca gtcagacaaa ggttaaagaa
     9481 gaagtatacc acaatgtaga ggctttagga tatgcagtat ttataccagt attttttgtc
     9541 agtgtaggat tagaagttga tttttctgac tttagtgaac aactactctt tatttctata
     9601 ttgacgcttg tagcgatttt gaccaaattg cttggaggtt attttggtgc tcagattgcc
     9661 ggtttttctc aacatagttc attgatggtc ggtgctggta tgatttcgcg tggagaaatg
     9721 gctttgatca ttttacaaat tggacagcaa agtcaattga tcgaaccaca gtactattca
     9781 cctttagtta tcgtgatttt attaagtaca cttatttctc cacttgttct caaatatttt
     9841 acaaaaaaag tttatgaagt gtgagtaaat caaaaagaag ttgtgaaaga gacatcgtga
     9901 aaaccgttta tttgaaaaaa ggactgtgac gataacgcca cagtcctttt actgtactta
     9961 aaaagcaatt ttttattgat aattttggtt gaggtattgc aatagttccg atgctcgttt
    10021 ggttgctgaa tcatcatctt ctttattttc caacatgctg accatcatat aaccttggtt
    10081 atcaagattg aaagcaaaca agaaactgtt ctcttgcccc tttacatcct gtttttcttt
    10141 gatttccgct gtaccagttt tcgctgccaa cggaatccct aaagcagaaa gagaatgtgc
    10201 tgtaccattt acatcttgca caacttctct cagatctggc acgatcgttt gtactgctgt
    10261 ttcgctgatg acattcttct tatcttttgt ctctttatct gcaatcaatt taggatagac
    10321 aagtgtgcca ttgttggtaa aaacagaata cattgctgct tgctggatag gagtaattag
    10381 aagttcgccc tgtccatatc cagtatcagc tagcaagata tctgagttaa agctatcttc
    10441 attagaaatt tgtgctggat tcatactaat tggcaaatca aggtcttcac caaaaatgaa
    10501 tttatctaag cctgtacgaa attttttctc acccattttc aacgtttctt gtgccgcata
    10561 tatattatcg gaatagatca aagcagtttt taagtctact tgtgatacat cactactgac
    10621 acgcgttgtt ttaaaagatc cccaagaact gtctttttgc catttaagcc cgttgatcgt
    10681 caacacttca tttggatcga tagtgccgtt gtcgagaccg attgctgctg taatcatttt
    10741 aaacgtagat ccaggagcat aacctgtcgc aaatcggctg atgaatggat gttcaggatt
    10801 ttcttcataa gctttgtaat cttcttgtga gatcccgttt gtcattttgt ttggatcata
    10861 gcttggagag ctagcaagcg caagaagatc accggttttt ggcgttgtcg caacagttga
    10921 tgcagctttt cctcctagac tgtcaaaagc tattttttgt gccttcgcat cgattgtcaa
    10981 tttgatatct ttgccatttt gaacttcatg ttcgatcaga acttttttct ctacaccgtc
    11041 tgcatctgta atgcttaatt tcccacctgt tgtcccacga agatccttat caaaagccat
    11101 ctccaaacct gagcgaccga tttttccatt actgcttaat tctggattct tatcaatatc
    11161 ttctgctgtg atatctccca cgtaaccaat cagctgagca gctgcttcac ccaaaggata
    11221 atatctgccg tctacttctt gaatggtagc tccagctgga agttctggcg ttgctccatc
    11281 aatgattttc aatgggacaa agtaatcggg ttgtacccag ctttgtgaaa tcgcctgatt
    11341 gatagcatct tctgttaagt cgaaggagga agcaatcgct ttgatattgg ctgttttttc
    11401 gcctccatct ccaagtttac ttggaacgac tcctaattgt tttagtttgc ctgttgttgc
    11461 taatggttca ccatttcgat ctataatatt ccctcttgct gcttcttgtg tggtcaggct
    11521 tactttgtca tttccttcca tttctggaaa aaccaagttg ggttgccagt tgatcgtggt
    11581 ttgaccatca tttctgtcta atgttccttt ataagaaaga tctttcaatt cacctaatga
    11641 ggtattcatc tttgctttat atgaaaaact gtaagtagaa tcgtcttttt tatctacttt
    11701 tagattcgat atctgaagtc ctttgacatc agcagcaccg tatatattct ggtatttatc
    11761 taagatctct ttttcagata atgcactttt atttgccgcc ttttttgatg tcatttctgc
    11821 agctttgtta taatctccct tgttcaaagc ttggacaaat tgctcaactg tcttttctcc
    11881 agcttctact gcttgggttt cttgatagtg ctggtagtaa aaataaccgc cacccgcagc
    11941 tgctattaag atcactgcgc cggcaatata agcgcctgtt cgatttttgc cgtgcttgtc
    12001 acttcttttc attttttcct ccttctcttg cttgtcattc ctttatttca ctatttatac
    12061 ctgtttattc taacatatgt ctttgaggaa atccatacca caatccagaa gcacactttc
    12121 tggaaggttc gtttggatac taccaagtaa tttcccaacc gctttgggta gcttgaatat
    12181 tgaagttacg tctttcagct gctgagagat agctttgatc acttgctgct gccttctgat
    12241 cctcccgaag tctccttctt tgtcttcccg aaatcgagca tactggagta aagtattacc
    12301 atccatcact tgctctcctt tgtttatata tacgccatct aaatctagag atttttctgc
    12361 atcgatcttg actccaccag gaaacagttc attaacgcaa tcgataaaat cttgaaatgt
    12421 gatacttgca taatagggag cttcaaattt gaaattttcc tttaatgttt gatttaataa
    12481 atcaactccc ccataagagt aagctgcatt tatcttattc attccgtaac ctggaatatc
    12541 gacatatgaa tctctcatga tagagatcag ttttgcttgt tgtgttttct ggttgtattg
    12601 tgcaaccaga agtgaatccg agcgacctcc accaatcgat cttttttcac cgcatctcct
    12661 cctcacacag caatccactt gtcaggtgag gagcgagttt gtctcatcaa ataagtcagt
    12721 cacaaaaata taaagaaaag gtcagaaacc ttttcgaaca agaattctga cctagcatca
    12781 tctcatcgtc cgagttttag cactatacaa cgtaaagaaa acattcttag ctattggaaa
    12841 aagccttaat ccaacagttt ctgttttacc cattggcgac tttcgacgtt tctgggcaac
    12901 agcctatgtt tgtataggag cctcacctaa caaggttatt actatattct atttacgagt
    12961 tcatgataga cttttcctaa tgattaggca atggaatttg aaatgaaata gtgaaattta
    13021 acaatcttta atatttttta gaaaaaaata acaaataagt tgaaagaatc ccgttgcaaa
    13081 ttctttcagt tgcaccagga ttctttgata attttttagt tttatgtgtt tttttatatg
    13141 ttttttcact tttcggaata aatatttatt tgttaaagta attaaacaat ccgctaatcg
    13201 tttgatctgg catttctatt tccttaccat ctaaagtaac agtagtaacg acaaaatggt
    13261 cttgcaatgg cggaacttct aagtactgcc cttgcagaaa atcattcact gtttcatatt
    13321 cttttttcat ttcttttgaa tctgtcgatg gtttatagct tatttttaac ataactagtg
    13381 ctccttttta ttcgggacgt gcaatcgtga aaatttctcc tattgctgca taatcatttc
    13441 ctgccaaacg gcttacagca ttcaattttc ttggatcgat ccttcctgtc ttatcatcat
    13501 agactgtttc atttaaatga tatttctgta cttctagcaa aaataaatca gctgttactt
    13561 tatttttatt ccgtatctcg acatgttcat ataaggatgt ttcaaaacga acttttgctt
    13621 ctgtcaatgc tggtactgaa atgattactg aatcagttaa agtcaagtca gtcaggtaca
    13681 gttcgctttc ctcattaggc agattggctg ctgttttatt tacttcttct acgttgtcct
    13741 catcaaggat atggataaca gcttctcttg tctcaagaat attctttgct gtgtctttta
    13801 ctgcatcttg ttttctctgg atcgccaagg ataagatggg tcgattcgaa ctgacgatat
    13861 tgaagtaact gaagggtgcg atattcacta tatcttttgc tgatttagtc gttactactg
    13921 ctactggtct agggatgact gaaccaatca gcaacttgta attttcacgt tctgtcaatt
    13981 ctgaaggact ccgttcaaac atggtttcgc cttctttcta taaaattcgt taaaaagcag
    14041 atcttgaaga caaaatgaaa agtaatgctt ttttcatctt cttagaatct gctttaaact
    14101 atctatcaat ttttcttttt tgtcaagaat ctgtgccgtg cgtgtttttt agttatttgc
    14161 tttacttgcg gcttcgacat atcttttagt gatcaattgc ggacgggtga taatagatcc
    14221 aactacgact gaaaaagcac ctaattcata tgctttctct acttgttcag gagagtcagt
    14281 atgtccttcc ataattactg ggatatcgac ttgttctatg atttgattca ataaatcaaa
    14341 atcaggtgtc tgcgtctttc gtgaatattc tgtatagcct gctaaagtcg tagacaaaag
    14401 atcaaaacca tcttcttgcg cttgaagcgc ttcctcaaaa gtcgaaatat ctgccatagc
    14461 taaccgaccg tgttcgtgaa tcatttggac aagttcagca gttgtttttt ctgtcggacg
    14521 tttacgagaa gttgcatcca gtgcaatgat ttctgcttgc gtctccaaaa gttcttccac
    14581 ttctttttct gtcgcagtaa taaacacttc tgacccatcg taatctcttt tgataatccc
    14641 tattaccggt aaatcaacga ttcgcttgat ttcattgata tcttctgtcg tatttgctcg
    14701 aataccaact gctccgcctt cttttgcagc caaagccatt ctccccataa taaaagaact
    14761 gtgtagcggc tcgttttcca aagcttgaca agaaacgacg actccgcctt tgatttgttc
    14821 cagtgtatcc acaatatctc ctccagtaat gttatataaa aatacagctt catataaaaa
    14881 tgtaaccctt ttctattcag gagacaataa tgagctattt ctttcagcag acctttctta
    14941 ctaaaagaaa attacttcaa attcttcgta tacgaccagc ttctcttctg taaattctat
    15001 gttcaacgct tctttataag catgtttaaa aaactcagca tggactttcc gccaataagc
    15061 taatgtccga tccccttctc cttctttcca agcatgctct ttcgtgactt cactaaaagg
    15121 caagatggtc acatttatat ttttgatgat acaaactggt tcattttttc cattcaggac
    15181 cacgtctaat tgaccaactt ttggtaaagg ctcattctct tgtttataca gatcgtaacc
    15241 acttgatgta ccagtcttta tcttttttac aactagttcg ctcaactcgt ctgccatttt
    15301 ttctgtatcc ccaaatgaaa agaaacctaa tttttctgat tgataactag gatatttttc
    15361 ttggaacttt ttgacatatt cttctacttg ctgatacata acatccaccc ctttgctttt
    15421 tataatgaaa aaaccatcca gatactagta taaacaatta tctggatggt gagcataatg
    15481 aattattgaa tattcaagac acgcagacta tcttcaacga tttgttttgg tgttaaatga
    15541 taacgttcat aaagctgccc tacaggaacg ctatccgtga attctttctc tgcaccaaaa
    15601 tttaatacgt tcattgaaga atcaccataa taacgactga tattttcgcc aaatcctcct
    15661 gacaaactac catcttctaa tgtgatgact aattcgtgtg tttcttttaa tttatctaat
    15721 gtttctacgt cattttctgt gatcgttcct ggattgatca acgttgcttc gattcctttt
    15781 tttctaaaag cttttactgt ttcttttcct aattcaagaa aaccgcctaa agctaaaatt
    15841 gctacttttc ctccattatt tactgtttga taggctggaa tactataatc atttgcgata
    15901 gctgtaccat gttctacacc atgtgtagga attcggattg ctacaggctg cgctgtttgt
    15961 tcgaacgccc aatctagcat agagatcata tcctcttttg ttgctggagc aagatatttg
    16021 atgttcggga tacttgataa gtaaggaatg tcaaatgttc cttggtgtgt tcggtcacta
    16081 gctgagatcg ttccgccacc gactaaaata atcactggat tctcgttgat cgctaaatca
    16141 tgtgataatt gatcatatgc ccgttgcaag aaagtacttg attggaagac gactggacgt
    16201 actccctgag aagccaaagc agaagcgaaa gtgatagagt gctgttcagc gatccccacg
    16261 tcaaaataat gatctggatg tttttcttgg aatgttttca gtccaaaggc gcctggtatc
    16321 gctgcattga ctgcggcaat aggagctgta cttgtttgca ttttcttatc caaataatcc
    16381 atgatgatca tattaaatgt ttctccagaa gttacttgct ttgcctcccc ggtggacaga
    16441 tcaaatggta catgccaatg atatttcatt ttattatcga ttgccggctg ataaccgcga
    16501 cctttttccg tatggacgtg aagtacaatc ggatgatcaa tatcttttac ttcttcaaat
    16561 aaacggatca ccgtttccag atcgtttcca ttatccacgt aacgataatc aaatcccatt
    16621 gctttaaata ggttattgct gcttttaccg ttggtttgac gaagctcagc taagttttta
    16681 tataaactgc catgattctc atcaatagac atctcattat cgtttactaa tacgattaga
    16741 ttggattgta aaacaccacc attgttcaat ccttcaaaag ctaagccacc acttagggag
    16801 ccgtcaccga taaatgctac cacatttcct gattctttct tcagatctct cgctttagct
    16861 aaacctgttg ctaagctgat agatgtagat gtgtgcccca ctgtaaaata gtcatgttta
    16921 ctttcatgtt ggttggaata tccgctcact tcacggtaat gtcctttttc aaaaccattt
    16981 tttcgaccag ttaaaagctt atgaccataa gactgatggg aaacatccca aacgatttta
    17041 tcctttggtg aatcaaatac ataatgaaac gcaatcgtta attctactac acctagattc
    17101 ggaccaacgt gcccacctac atcgctgtcc ttttgcaaga ttttttggcg catctcttct
    17161 gccaactgtt tcatttcctc aacagataat tttttgacat ctgcggggct attgattgca
    17221 tccaatacat gtaatactgt actcataata cattctctcc tccgtactat cttcttttaa
    17281 tgaaaataga tttgttacac tttatctatt ctactactta gagttgactc taagtcaata
    17341 gcattttaaa aaaatttcag agaaagcttg ttatataagt agatcttcgc acgaatcagg
    17401 actcaaataa aaaccttcta cagacaatta aaaagctttg tagaaggtct cctaattaag
    17461 gtctcatcaa gaaatatgtt tttttaatag gtgaagttcc tgagcaatat gatacaaatg
    17521 gatgtttgca ccttttaatg agattggata gatctttgtc ttttcagttt ctaatccatt
    17581 ttgatagcta gcagcatcgc acaaaaccac tctttttttc gcacatagac attcatcagg
    17641 caattcttct aatgaacgaa ataaaatagg atttatttca gcgatgcctt cttccgcata
    17701 caaatgaaat tgggttcgat aaaaaaattg tttgactgaa ctttctgaaa caacaaaaat
    17761 ttctatttcc tgtctccccg cttgttcact gttgatcaaa gaaagacttt tgacgaattt
    17821 tcgaatgaaa ttttcattcc atcttagttg gatttgattt ttctttttcc actcttccaa
    17881 tatttctaca atttttccgt acagaagttg ttctttttct gatagaagat agatcctttc
    17941 cggtaaaaat acttgtccat ctgcccacaa cgtacggaca aattgtaaaa aggattactt
    18001 gaaaataaaa ttttctgcat cgtcgatttc agcgatacta ataatctgta aaatcaattc
    18061 attcagtgat aggtcattga gaacaaaatt tttataaaca acttcgaaat cttttcttaa
    18121 cagctccatg ttctcattgg tataatttct actgttgaac aaagaaaaaa tataatagac
    18181 atcttcttct tgtaagtcta gaattccttt ttgctcttcc aatccttgac agactaattg
    18241 aaataacggc ttcgtttctg cgcttttctt atcttcttct ttcagtccaa ggggaaatgc
    18301 ctttcttccg attgcgatct ctattcctcg tatgataaag gacttttcat cttccgaata
    18361 tcttctttta aaaaaatgag tctctacata atcaatcaac tcgttacaag cttcttctac
    18421 atacggattt ttattttcat agccttccca attgacataa cgtactaaag ctagcaagat
    18481 gttccgcgag tcaaattctg ggataatgat ttctttattt ttcaaataat cattttcttt
    18541 aaaaaaatcc aatacgattt ttttgattgt atatactttg ctcaaagaaa taaattcaag
    18601 atcagccagt tctgtactcg tgaatatacc attaaaataa taacttaaaa actttaaaaa
    18661 ataagattga ccgtacagaa actgtgtgag ctcagaaaga gcaataccat gtttgaatac
    18721 gagtttatac ttatgcttct ctcttacttg ttcttttata caatattcta gacactcggc
    18781 tagactttct agatcgtgaa ggatcgttgg tgtagtcacc cttagagaaa tagctgtttg
    18841 ttcgatgtct atttcttgga attcaaataa ttgttcacac agatacactt ttctaaatat
    18901 atttcgctcg atgtaatttt ccagcattcc tgatacacgc ctccagtttt tcttatgatt
    18961 ttatttaatc atatctgtaa atcgtatttt tgtttccttt ttatttagaa aagccctttc
    19021 aaaaaccaat aaaaaaagaa actgggacaa aagtcaaaca agctcacaat ttcagcctca
    19081 ttgtaggtag ctttgagccg tttggacttt taccccagcc tctttcaaga atggttattc
    19141 aaagatgtca gcgtctagtt ctttgactac atctttcaac aattggatac cagctgcgct
    19201 gttaccaaat ggatctagtg ccgggctgaa gacacctatg ccgtaacgat ttggcacagc
    19261 tgccatcaac ccgccgccta caccgctttt tgcaggtaca ccaacatgga cggaaaactc
    19321 tcctgattca tcatacaaac cagcagttgt cataattgat ttgacgattg tagcactttc
    19381 ttcagtaatg atttgtttgc cattccaagg agcaattcct ttatttgcca gcacagcagc
    19441 taattttgct atgcctgtag cggtgacatt gagggagcat tgtctgaaat aagcatcgag
    19501 tatctccggt acgtctcctt cgatcatttg attacctttc atatagtaag caagagagcg
    19561 attgatatca cctgttcttg attcagaatg atagatttct tcattcaatg tgatattagg
    19621 atcatcacag atctctttca taaatgacaa gattcgctca aatttttcat ctgcatcttt
    19681 accggcaatc aacgaggttg ttgcaatcgc tcctgcatta acaaaaggat tcattggatg
    19741 tttgcgatga ttgatttcca tattcatgat agaattgaag gcaaaaccgg ttggttccgt
    19801 gttgattgtt tgaaagactt tttctattcc gttgtctttg attgctaata acaatacggg
    19861 aacttttgac atgctttcga tggcaaagcg cacatcggcg tctcctccct caatcagttg
    19921 ttttgtatcc aaatcataaa tcgccatacc taattgtttg ggatccactt cagctaatgc
    19981 tggaatgtaa tccgccactt gtcctgtttg ataatagtgg aaattttttc caatcaattg
    20041 ttctaattct tttacttcca tttgaataac cagctttcat tttttatttt tctgcatgct
    20101 ggaatttatg acggcatgcg tagatcgtaa atggaataac taagacgata agataagaaa
    20161 caatcagaat cgtttcataa atcccatgct gtccagctgc gatactagca ggtggaacaa
    20221 atgagataca aaaagcaaaa agtgaagtca aaagacctaa acaagcaacg attactttta
    20281 caacttttcc gcctggaacc tgataggctc tttcaagact gtcatcttta aaaaccagct
    20341 tcaaatagcc cgcgaaaaac ataaagtaca acatcaggta gattacaaca gtcaatgaca
    20401 tcgctgcaag aaatgaaaga ttattccccc cgccgcttaa tgtaaagacg gctgcccaga
    20461 ttgttgcaac gatcccattt gcgatcatca aatgagcagg aacaccgtgt gtgttttctt
    20521 ttgcaaaata ttcaggaagt aatccttctt ctgctgctac gtgaagtgct tttgaaggag
    20581 aggttaccca agaagcaatt tctccgatga cacctaaaca aacaagaatc gcaagaattt
    20641 tcgcaatcca tgcaagattt ggatcaatgt gtcttaatag gaattctaac gattgaatca
    20701 cccctgtatt aagagacaaa ccatgttgag gtactgtagt agcgacggtc aatccgccaa
    20761 aagtatctaa aatgattgcc agaataacca atacaaacat tgctaatgga taatcctttt
    20821 tagggttttc catttcattt gcatgactgg cagatgtttc tacgcccata tatgctaaaa
    20881 taaaggaaac aaaaacaacc agcgtaggca atttcgaaaa atcaggaatc aatgttttca
    20941 tagacagtat cgtttgtagt ggagcgcctg aagcgacata atggatccct aagccaaaca
    21001 agactaatgc tggcaacaag ataccagcta caaaaccgat ccgagcaatc aatgaagtgt
    21061 atttggttcc ttttaactgt gagagtaaaa tagcccagaa aataatcaat aggattgcca
    21121 atttcagata tttattgttt tgtatagcac caatacccgt tgcataggat aatgcaccag
    21181 ttaagaaata cagcatggtc aagtagccaa ctgttatctc aaaccattgg aaaaagattg
    21241 cggcaaaacc gaatcgtttt cccaatgtgc gactgaccca agtatagaca ccgccttttt
    21301 cccatccttt caccgttgcc atctctgctg cgcacaatgc cacagggata aaccatagca
    21361 aaccgcctaa taacaagaaa aatactaagc tgaagcctga ggtcgcgaat gtgggatatt
    21421 catataatga aacgaccatt gagatggtca tagccaaaaa gccaaagaac gttaattttt
    21481 ttgcaggaat tttcgttttg ctttgttgca tgaatcttcc tcttttctat ttgaatttgt
    21541 ttcaattgag tttgttgcca aagaagaaaa agtccaatcc ttcttcttgt tcttcttttg
    21601 tagtagtgat cgttgtaaag tgcatcgccg attttaaaat cactgttgtc agcacatagg
    21661 catacacggc agtaatgatg acaccaatca cttgaatccc taagaagtac cagctgcttg
    21721 gattcgttag taaatggctg ttagatgcaa agatccctat cagaatcgta cctgtgaatc
    21781 cgcccatgcc atgaacaccc cacacatcaa gtgcatcatc ccattgtttc tttttacgaa
    21841 aatcaacagc agcatgacag acgatacccg caatgatccc aatcagcatg gcactttttg
    21901 cttctacata accggcacaa ggcgtaatgg ctgccaatcc agcaacggat cctgttagta
    21961 cgtcgacaaa atcaaagcca tttcctttta cttttgcaat gatcagccaa gtaatcatcg
    22021 caaatgcaag agcgatgaat gttgaaccaa atgcattgac tgcttgttca tctgctcgaa
    22081 gtgcaccacc ggaattaaaa ccgaaccagc caaaccaaag cagtcctgta ccgatagcag
    22141 ctgccataag actgctatgc tgacttcttt ctcctaaagg aatctttctt tgaccaagtg
    22201 tgaggataca tgctaaagag ccgaatccag ctgtcgtgtg gatgactgct ccaccagcga
    22261 aatctctaaa tcccatctga tccaaaaatc cgccacccca tatccagtgg caaacaggga
    22321 aataaatcag cagattccaa aaaataacaa gtataatata gccttttaaa ttcaatctcc
    22381 ctgcaaaagc tcctgtcatc aaaggaacgg taatcacgat aaacatcagt tggtaaagaa
    22441 agaacattaa aaatggaatc gttgcaccat gaaacatgtt ggggaagctt gtaacatgtc
    22501 tcatcaaaaa gaaatctgcc gggtttccaa taacaccgcc gatatcccgt ccaaaggcca
    22561 atccgaatcc accaaatacc cacataatcg ttgttatccc aacggagaga aaagattgag
    22621 acatgatcgt tagtatatta ttcttccgtg ctaatccgcc gtaaaaaaaa gctagaccag
    22681 gagtcatcag gcaaaccatt gctgtacata aaatcatgaa tgctgtacct cctgtattga
    22741 taggtaatgt attcattttt ttcgcctcct ttctacgatt acgagtataa aagaaggtgt
    22801 agtt
//
LOCUS       DACDEG010000037        22502 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.039, whole genome shotgun sequence.
ACCESSION   DACDEG010000037 DACDEG010000000
VERSION     DACDEG010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.039"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..575)
                     /locus_tag="IV142_001457"
     CDS             complement(<1..575)
                     /locus_tag="IV142_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8485123.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPS"
     gene            complement(1143..2210)
                     /locus_tag="IV142_001458"
     CDS             complement(1143..2210)
                     /locus_tag="IV142_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8485124.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(2230..2709)
                     /locus_tag="IV142_001459"
     CDS             complement(2230..2709)
                     /locus_tag="IV142_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HAP8485125.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(2726..3187)
                     /locus_tag="IV142_001460"
     CDS             complement(2726..3187)
                     /locus_tag="IV142_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8485126.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(3201..4451)
                     /locus_tag="IV142_001461"
     CDS             complement(3201..4451)
                     /locus_tag="IV142_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAP8485127.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(4829..6280)
                     /locus_tag="IV142_001462"
     CDS             complement(4829..6280)
                     /locus_tag="IV142_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP8485128.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(6601..7365)
                     /locus_tag="IV142_001463"
     CDS             complement(6601..7365)
                     /locus_tag="IV142_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP8485129.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(7393..8091)
                     /locus_tag="IV142_001464"
     CDS             complement(7393..8091)
                     /locus_tag="IV142_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP8485130.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(8103..8882)
                     /locus_tag="IV142_001465"
     CDS             complement(8103..8882)
                     /locus_tag="IV142_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP8485131.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(8898..9842)
                     /locus_tag="IV142_001466"
     CDS             complement(8898..9842)
                     /locus_tag="IV142_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8485132.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(9888..10667)
                     /locus_tag="IV142_001467"
     CDS             complement(9888..10667)
                     /locus_tag="IV142_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485133.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
     gene            complement(11047..13122)
                     /locus_tag="IV142_001468"
     CDS             complement(11047..13122)
                     /locus_tag="IV142_001468"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP8485134.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(13124..14041)
                     /gene="glyQ"
                     /locus_tag="IV142_001469"
     CDS             complement(13124..14041)
                     /gene="glyQ"
                     /locus_tag="IV142_001469"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP8485135.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(14427..15245)
                     /gene="recO"
                     /locus_tag="IV142_001470"
     CDS             complement(14427..15245)
                     /gene="recO"
                     /locus_tag="IV142_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP8485136.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(15388..16287)
                     /locus_tag="IV142_001471"
     CDS             complement(15388..16287)
                     /locus_tag="IV142_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP8485137.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(16302..16703)
                     /locus_tag="IV142_001472"
     CDS             complement(16302..16703)
                     /locus_tag="IV142_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP8485138.1"
                     /translation="MPMDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(16681..17157)
                     /gene="ybeY"
                     /locus_tag="IV142_001473"
     CDS             complement(16681..17157)
                     /gene="ybeY"
                     /locus_tag="IV142_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP8485139.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(17186..19378)
                     /locus_tag="IV142_001474"
     CDS             complement(17186..19378)
                     /locus_tag="IV142_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP8485140.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNTGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(19402..20373)
                     /locus_tag="IV142_001475"
     CDS             complement(19402..20373)
                     /locus_tag="IV142_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP8485141.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            21078..22457
                     /gene="brnQ"
                     /locus_tag="IV142_001476"
     CDS             21078..22457
                     /gene="brnQ"
                     /locus_tag="IV142_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP8485142.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         6334 a         4702 c         3739 g         7727 t
ORIGIN      
        1 tctgacggaa acttgatatt gtgctgttga attatctcag atgaatacag cgcatgccaa
       61 actgacattg gcaatataaa atcgacttta taactcggct cagtgccaat cattccaagc
      121 aacacttttt caattatagc gttatttgta aatatcgtac tatcatatct tgctggcttt
      181 gaaataatat tattatcatc ataatattca taatatccag cataacatgt gtctgcatta
      241 ctccttttta taccatttaa cattgcttca atataattag ctgcaacaaa atcatcagaa
      301 tcaataaaag caacatattt tccttttaca tgttcaagtc ctgtatttct tgccaatccc
      361 aagccagcat tatttttatg aataatacga atccttgaat cctttaatgc atagtcttca
      421 catattttat tagatgaatc cttagatcca tcatccacaa ttataatatc taggttctta
      481 tatgtttgat tacacactga ttccaaacaa cttcctacat acttttcaac attatataca
      541 ggtatgatta cactaattag atcactcgaa ttcatccttc tatcctttca ctcacgtttt
      601 ctcaattagt caatgacggc atcaacgatt ttcttaaatt ctctgaaaaa aatagactct
      661 gatcttgatg atgtgaatga catactatct tcttctagaa aaattatttc ctttaaatta
      721 tttattaaat caataatatg atcctcgtaa gtatgctttc ccttggaata atgaaaatat
      781 acttttgtat tacacaatat tgcaatttta tttcttaata tgattatttc actcattcat
      841 tactgccaca tatacacaac ttataaccac cccctgcaaa gtttagggta agttatcaga
      901 taatatcttc ctcttcatca tccagaatac aaaatctatt tatttataga aatatcatta
      961 atgtttttat ataatgtacc gtgcttgtac tcttgctcta ttacaagcac taaaacacta
     1021 ctattcattt gaattcttac acggataaaa atttacatcg tctatttcaa tattttttct
     1081 ctctatttta ttcacttaat aagttcatta cataatcgaa acatttttta ttttccttgt
     1141 catcactcct tgctcccttt ttgatacaga tttaaaattc tatcaaagaa atttccatcg
     1201 ttaaatttcg ttattgccaa atccttcgaa caacgtgaaa attttacgta tttatcttta
     1261 tcattgattt ttagtattgc agatgtcaac gctgatacgt caccaggttg aaacaaatat
     1321 ccattttctc catcacgtac tgctgaagaa atatctccta catctgtagc aattacaggc
     1381 attccataag aaatcgcctc tagaatagat ataggcaatc cctcgttata ggacggtaaa
     1441 atcatcacat ttgatcttct atagcactcc tccttttttt ctcccacaac ccaacctgta
     1501 aattcaaaat acgattgcaa attatattct tctatcaatt ctttgagcga tatttcttca
     1561 tcacctgctc cagcaatctc aaaaataaac tttttttctg gatattgttc tgcaatgttt
     1621 ttaactgctt ctaccaaatc gtgtacacct tttcgcttta tcaaaactcc cataaacaga
     1681 aatcggcaca tttttgaatt ccactttatg gtatattctg gaactacaat agaattttct
     1741 acaacaacag ttttacaatt tggctcaatt tctttaatta tcttcttcca tttttctcca
     1801 agtacaatcg tggaatcaga ctctcttaac agcaagcgaa tctgattttg tttttttaca
     1861 gaaacactat cataccattt tttaaattcc gatccatgaa gatggatgat tacaggaata
     1921 tttaataatc taaaccactt attcagcaca tatttcctac taaaactccc tctgtacgac
     1981 atgtgcatat gaataatatt aggtttactc aataaagaga tacctataat tttagcaaat
     2041 gcatacataa aatataaaga tttaaatatt gcatttccac ccttataact cggtataaaa
     2101 gtcatctcga tattcttttt ttcccaatta aaattgagca tttgtgtaat tatacttgta
     2161 attccacctt ttaccgagct gtcattacca atcataagta ctctttgcat aacgctttac
     2221 tccttatatt tatatttttt ctattaatac ctctatcgaa ttaataatcg tttccgtgtt
     2281 agatacatac ttgttaaaca tccgaactct tgtttcttta acggcatctt ctaaattttc
     2341 taattcataa caaggataga taatattcat ctcatcaaat tgtctaatta actgcagttg
     2401 gtgatcatcg acatgctcac catacttagc caatcgagga acagcaatga cccttttacc
     2461 tttttttacg gccccaatta tagcacctgt acctccatga gtaatcacca tttctgcttt
     2521 ttgcatagtt tcagtaaact catccctatt tagaaatgct ttataggaat aatttagtgg
     2581 tttataatca ctataaccac tctgtgcaaa tgtactttcg attattactc ctttttcaat
     2641 taaatcatca atagctttta acaatctatc aaattgaaat ttttgtgaac ctaatgttat
     2701 aaatatcatt tttttctcct attatttaat atatcccacc caaataaata gcattaggat
     2761 aaaattgtag catagtttcc cactgaacat aaaattgatc tgcaaaatga tacatcaatt
     2821 ttcctgtctg agtagggctg gttacttttg caaacgattc aatataaatc agtttttttc
     2881 ccatcatttt tgttattaag cagataggga tcatagctaa aacacctgta caaatcacga
     2941 gatccggttt ttcctttata tatataataa ttgatttgaa taaattaata agtatagtac
     3001 acaaaaaaaa cttttctttt ctatttactt gtctcatgta gtacgttttt tcgccttttg
     3061 actcagctga atattctgtc tgttctgtta taacaaaact ctcatacttc tccatcagtg
     3121 gttttaacat cattaactgt tcataatgcc ccccagagga agccgcaaaa catatctttt
     3181 tgttcatatt aacctctcta tcagtcacgc tgaaaaatat ctctagtgta aactttttct
     3241 ttaatatcat ccaagcaggt atcatatcga ttggcaataa tagaatcaca catcgcctta
     3301 aactgctcta aatcgttgat aaccttactt ccgaaaaaag tctctccatc tttcagtgca
     3361 ggctcataaa tcaccacagt cgcaccttta gcttttacgc gcttcataac tccctggatt
     3421 gaactctgac gaaaattatc actgttgctc ttcatggtca gccggtatat accgattaaa
     3481 actggatttt ccctgttgac atcatactga ttatattcag cgtagttgta atagcccgcc
     3541 atctgaagaa cacgatctgc gatgaaatcc ttacgggtac gattagattc aacgatagcc
     3601 tcaatcaaat ttccaggaac atctgcatag tttgccaaca actgcttggt atcttttggc
     3661 aagcaataac ctccatatcc aaaggatgga ttgttgtagt gagttccaat acgaggatcg
     3721 agacaaacac cattgattat ctgctgagta ttcagcccct tcatttcagc ataagtgtct
     3781 aattcattga aatagctgac tcgcagtgct aaataggtat ttgcaaacag ctttactgct
     3841 tcggcttcag tgaatcccat aaaaagagta tccacgttct ccttaattgc acttttctgc
     3901 agaagtgttg caaaaacatg agcagcattt aacaagcgct catcagtcat atcagtacca
     3961 acaataatac gagatggata cagattatca tataaagcct tgctctcgcg cagaaactct
     4021 ggactaaaaa taatgttctt actccccgtt gtctcacgga ctgattcagt gtagccaaca
     4081 ggaattgtag acttgatgac catgatggta tcaggattca cgtccatcac aacctcaata
     4141 actgactcaa cagcagaagt atcgaaatgc tgagtacgac tgtcatagtt agtcggtgta
     4201 gcaataacaa cgaaatctgc ggatttataa gcgctccttg catccaaagt agcagttaag
     4261 tcaagttctt tttctgtaag atacttttca atatattcat cctgaattgg agacttccgt
     4321 ttattaatca gttcaatctt ttcagggata atgtctaccg cggttacctg gtgatgctgc
     4381 gccaatagtg tggcgatact caaaccaaca taaccggtgc cggctactgc gatgttgtac
     4441 tttttcttca caatatctcc tctttcctct cttaagtatg gagaatgtta ctcctacggc
     4501 tattcatcat gtcaaaataa cacagcttag aatgttgata aaataatttt ccagaatgtg
     4561 ataaaatctc tttcatttat aaaccacaca tctaatttta tctattctcc acaagacatg
     4621 tagagtattc tttaataaca agatacgacc ttactaaata cttcaaaaaa ttatcatatt
     4681 taattcataa atataaatct tcattttaaa tgtatgccca aaaatttatc tgtaatgcgt
     4741 catcaaaatc tagattccaa atctttatca atttttatcc actcattcta ccactttagt
     4801 aatattttct atttataaca ctttcgtcct acattgatcc ccccctctga agtacagttt
     4861 ttactgtctt aaataaaatt ttaaaatcta accccatact ccaatttttt atatattccg
     4921 tgtcaagttt tgctacttct tcaaaatcag taatatcact tctaccactt acctgccaca
     4981 ttccagtaat tcctggctta atagcaattc ttgcacgatg atgataatca tattcttcat
     5041 actctgattt aaggcatggt cgaaatccta taaggctcat ctttcctgtt agaacgttga
     5101 aaaattgagg gaattcatct aaagacgtgg atctaataaa ttgaccgatt cctgtctttt
     5161 ttgttccatc tggaagtatt ttatttccga taattcttgg atcaaactcc atcttaaaca
     5221 taagattatt gctcatctta ttttctttca ttaattctac ttttctttct tctgcatcca
     5281 tatacatact tctaaactta tacatcttga acggtttacc attctgacct atacgttctt
     5341 gtgcaaagaa aattgggcct ggtgaattaa tataaataag tggagcaata aagataaaaa
     5401 taattcccgt tgcaatacat ccaacaatac ctgcaagaat atcaattgtt cgttttataa
     5461 atgcctgttg agttgtcata acattgatac ttgttgtaag tactgtatat tctcctatac
     5521 gaccaataga ttgttttccc aaagcactat ctgatacttt tgcaagattc aaatgtacag
     5581 taaggcccat ttcagagcat cgttcaataa attctttcat acacgaagta cttttattca
     5641 aaataataaa gacttcatct acccatccct gacataagta atccgctgca tttttggaat
     5701 cagaaacaac tgtcactccc tcaacatctt caccaatcat gcttttatct atgataatga
     5761 caccatttat tttatacatt ttatagttat gctctttcac attacgaata actacttcag
     5821 caatactact agatgtaact ataataagtg atttgttccc ctcatttttc attttatgtt
     5881 tcaagtaatg tttccataag aggcgagtta tatatgtaat tacaacgtat aaacctccca
     5941 tcaagaaaag aacgactcta gaataatcat ttccaccctg aacactaatg agatacaaac
     6001 ttcctaacag caccaacata atcgcttgct ttacagaaga agcaaattct atatagtacc
     6061 ctctttttaa tacacctcta tatgcttcaa acatgaaaat aactaaaaga tctgccagtt
     6121 caatgaagac tgccatgttt tgatacattg gaatcacata aggattccaa tctacctggc
     6181 gtaagacata cgatagataa aaagcaagct ggatacacaa taaatctaaa ataataaaat
     6241 caatatgttt aacccatccc cttgaacttt tcttgtacat tcctttttac tcccctcttt
     6301 ttaataaaat aaattgtatt acttgttttc tctcctaaat atctttctga aggattattg
     6361 actcgacaaa ttggcttgtg gatattctta tatatagaac atccgtaagc caacttaaaa
     6421 ctccttaaca aaattttttt actcatattg tagatcaaaa caagtatcca agaaatcata
     6481 ttttatttct tttttctgct tactcaacaa cagctaaaag aaaaatcatc ataaacaaat
     6541 tcaactaagc caacgatccc cttgtgaaat caagtttttt cttgatttac gaataaagag
     6601 ctaaaataaa ccaaacttct tcttttttac ctcttcatag actggataac tgatttcatc
     6661 cccgttgatc agatcctttg cgacttgttt catctgtact actttttcct taccaaagtc
     6721 ctttgcaatc tgctcatagc actcttttaa gtaaaaggtg cgcttagaga ccccatgggc
     6781 atcagaagca gccatctgca cgagattatg tttgaccatc gttttggctg ttttctggat
     6841 gtctttgcca aataccccaa cataacttgg cgcagtgagt tgggccaaac atcccatatc
     6901 taggaacgga agcaaatgat ttgggtcttc gcgaaaatgc acgttgcgtt ctggatggac
     6961 gattactggt gtctttccta gttctaacaa ctcaaaaaaa agccgttctg catataacgg
     7021 cacttccagt gtcggaaatt cgatcagaag atatgtatca tctaaatctg taaataatat
     7081 ttcatctcgg tggatgtctt cgatgagcgt tccggtaata cgtacttctt gtccttccaa
     7141 taacgtcaaa ggcaactgtc ttttctctaa ttcggcttgt aacgaagcga ctagagaaat
     7201 gacttgtgat ttttcattgc tgtattttcc attattgtgg tgtggtgtgc ataaaatatg
     7261 cgtgatgcct tgctgaatcg ctttttcagc cattgcaata cttgcttcta agttttctgc
     7321 cccgtcatcg attcctggta ggatatggca atgtagatcg atcatttctc ttgtctcctc
     7381 tcttcttatt ccttaatttc cgtagtaata accagcatct ttgctatgtt ctgctccgtt
     7441 gtacacaaca cctaagactc gtgcttgtac catctccagc agttcttttg ctttagtcaa
     7501 ggattcttta cgtgacatat tttcacgtac gaccaataaa gtgccatccg tttttaaaga
     7561 catgatctgt gcatctgtta cggctactac tggtggcata tcgaatatca ccacgtcaaa
     7621 taactgtctt gcttcggata ggatctgatc cattcttggc gagcttaaca attctgatgg
     7681 gttgggtggc ttaggtccac taggcaaaat cgacaagttc tcgactggcg ttcgttggat
     7741 cacatctgct acacttcctg atgaactaag tgctgtactt aatccactgg cattgttcag
     7801 ctggaatgtc ttatagacga cgggtttacg taaatctgca tcgactagta gaactcgttg
     7861 acctgattta gcaaaaacga ctgcaatgtt tgctgcagtg gtggatttcc cttctccagg
     7921 acctgatgaa gtcacgacaa tcgttttgat ttgctgacca gctgcggagg caaactggat
     7981 attcgtccgg atcgtacgat attgttcggc gattggtgaa gatgggtttg ttaatgtgat
     8041 caaactgact gcatgagttt gtgctgcttt ttgtttctgt gttcgtgcca tttttcttcc
     8101 tcctagactc ttgagcggct tctgcgtgat ggggctgttt tatcgtttgt agcttttggt
     8161 cgtgcaggag ctgttccttt tttacggata ccagcgttca attctttcgc tgacatttct
     8221 ggaactgttc ctaagattgg gaatcctaat gtatctgtca catatttttc atctttcact
     8281 gtccgatcga gtaattccaa taagaatgcc agacctacac ctaccatcat tcctaacatt
     8341 aagccagtca ttaaattcaa tttattattt ggcgaaacag gtgtcgtatt ggcaactgca
     8401 cctgaaatga tcgagatttt atcaacattc atcacatctt ttgcgttctc ttggaaaact
     8461 tgtgaagttg tgttagcgat ctgttgagca gccactgcat tcgtacttat tgcctgaatc
     8521 gagaacattt gtgaattttc tgcctgatta actgagatgg catctttgat ttgttcagca
     8581 gtcattttca cattgtcttc tgtttctaaa cgatctttga cttcattgag cactaaatca
     8641 ctgacgatca tatctttata cgtattgatc atttgcaggt tcgtattgac atcattgaca
     8701 tttgcagtgt ctgattgtgg cagtgtcacg atcaattgtg cttgtgaact gtattttggt
     8761 gtcaagatga aaaatgttac gattcctgag atggccaagc cagcaaacat tgagatgatg
     8821 attattacca agtgtttgcg taatgtttca aaaatttctt tcaagctgat tgtctcttcc
     8881 attttttctc cactttccta tttattctct agattcattt gatcttttaa ttcttttttc
     8941 acacgatcaa gttcttgatc actgactttt tgataggaga tattatcttg catgaagcct
     9001 tctccttgca gctgatcttg cttaatcgta ccaaatgcct cccgataatc taaagcaatt
     9061 tttttcattt catcaaagtt catgtctgtt ttcatattcg actctagagc attcaaaacg
     9121 gattgatagt tgctgacacc gtttagactc aatacttttt tcgctacgcc ttctacgatc
     9181 tttctttggc gcccttgtct accgtaatca ccatttgggt cttcataacg catacgagaa
     9241 taagataatg cctgatcccc gttcaatgta attttaccga ttggaaagtc atagccatct
     9301 tgtgagaaag tgaggtcatt attgacttcg ataccaccaa ctgcatcaac gagttccttt
     9361 agtcctttca tattgataga gacatagtga tcgattggga tgtctacata tttttgtacg
     9421 gtatccatcg ccatggcagc tccgccgaat gcgtaagcgt ggttgatctt gtcttttgtg
     9481 tcacgaccta cgatgtcgac atacgtatct cgaggaatgc tgacgatcgt tgtctgtttg
     9541 tcttcaggac tgactgtggc aaccatcatc gtatctgatc ttccttgatc gacccgacct
     9601 aaatctccag tatcaatccc catcaataga acagagaaag attcttgttt gtccagatca
     9661 actggtttcg ttcgttctcc ttgggttcgt tccactgatt cgtaagtttt ctcaatagaa
     9721 tgtgagagat ccagatataa ttttgctcct actcctacaa ttgctaatac taatacgata
     9781 agaactgcta aaataatcaa aaatattttt ttccctcttg attttcttct tcgtcttgcc
     9841 atagtttcct ccgtttgcta accttgcttg aatcaacgtt ttaactgtca atagcttata
     9901 ttgctatagc ccatttgctg agcaacagtt tgtgcttttg ttgcgtatgc ggctcttatt
     9961 ttttcattcc ggatctgctc tatttgactg agcgccgtca aatattgatc gtaagtagcg
    10021 gtagctgtta cttgaccgtc tttcagcatc gtatcgaata gctgatctag ttgcgtgact
    10081 tgtgcaattt gggtatcagc gaatcctaga tattgcataa cgactgtttt ccaagcacta
    10141 tccttaaaaa agctcatatt ttcacgtaca tgggcgacat ctgcacttgc caacttttct
    10201 ttgatgatga catcatccac agcttgctgc cagtttgata cgtttttagt aaataattta
    10261 ttgacagccg tctggatctt cagttttgat tctgcatcct ctaactgcgt taagactgct
    10321 ttcttttctt cttggatcgc agcagcttct ttaggtaatt ctgattcctt gatgtcataa
    10381 tcatctgccg aaagtttgat agaagatagt ttatgtgaca aatttgaaac tgtttcttct
    10441 gttatttcag gatttaggaa aattttttct tttgtactgt acagcttatc tacttctttt
    10501 gataaagagg tcacggtgtc tttttgattg gtgatggcag attctgcgta attgatccgt
    10561 tgatgatatt gccgctgatt attgagatgg tatccaaaca agcaaatcac aatagcgcct
    10621 gtgcctaccg ccacagtagc caataacttg acgaacggct tttccataga ttcactccta
    10681 aatattgatg atagataagt aaagatgtga ttcattcaca tcagaatgag cgctttttat
    10741 tttaaaagca taattaaact aatatattca tagaaatatt tataaacagg ttttccctat
    10801 ataatcatac cacatacagt ttttttgact agggaaattc ctaatttaaa agtaaataaa
    10861 acagtaacaa gattataatt aatcgttttc atttttattt cctcactttt catgagcgtt
    10921 ttgaaaattc gaacaaagaa taaaataaca aaaattatta aaatagaatt aataacagct
    10981 atttataaca aaaaaaacag agacacagca cgtttcgtgc atgctctctg tttttcaaac
    11041 aaaagactat tttgtgatga tttgatccaa gcttgctaat gacaaagcca tggcagctat
    11101 ttgacttagt tgtgttagtc ggttcttacg tactgctggt tcatcagcca tgaccatcgt
    11161 ttgatcaaaa taattttcga tcagtggacg aagagcgaca agctggtcgt agttttcgtg
    11221 gatcgtttgg tcagcaaaac ttgctttcat tgcttctact gctttgtgta attctttctc
    11281 tgcgtcgttt tcgaacagct cagggtctac ttctgattgt ttttcaagct gcgctttttt
    11341 cgctaagttc acgacacgtg tcaaagcttc cattgaaggt ttgaagtctt catctgcaag
    11401 atgttgtttg aacaattgtg ctgctgcaaa tacttttgtc agatcttttt gttccgcatt
    11461 caaaacagca tcgatgacat catgacgaat attcttcgtt gtcaataatt ggcgcagacg
    11521 gcctttgata aagtcgatca cttgttgttg cccttctgat aaaccaatgc cgaaacgatc
    11581 cgtatcttta ctgataacat cgctaatcgt ttcttgcaga acagacaaag gaaaggccca
    11641 ttttttcgat tcgatgatcc gaacgacacc gtatgcttgg cgacgtaagg cataaggatc
    11701 gtttgacccg cttggaatca tcccaactgc aaagaaagaa aagatgctat ctaatttatc
    11761 tgcaatcgct aaaacagaac ctactgtaga ctgaggtagc tctccttcac tggatatagg
    11821 catgtaatgc tcacgaatcg ctgtcgctac agcaggtttc tcgcctttca gaagagcata
    11881 tttttcgccc atgatccctt gtagttcagg aaattcccct accatgttgg ttactagatc
    11941 aaatttatag atttcagcag ctcgttgcaa atccatcaat tcttcctcag atagtcctac
    12001 ttgttcgcca atgatacggg caatgacttg tacccgttgc attttctcat agatactacc
    12061 gattttttca tggaaagtaa cagtttttag tttttcaacg tattggtcga ttgaagcttt
    12121 cttgtcttca ttataaaaga attccgcatc ttctaaacga gccgtcaata ctttttgatt
    12181 gcctcgaacc acatcttcca attttacccg atcaccatta cgagcagaaa taaaatgagg
    12241 aagcagcata ccttgatcat tccgtacctc gaaataacgt tgatgttctt tcatagaagt
    12301 gactagtact tcttcaggaa cagaaagata tttttcttcg aagtcgccag aaaagacagt
    12361 tggatattcg actaaattgt tgacttcttc tagtaaatct tcgtctagat caataaccca
    12421 gtgatttttt tctgctaatt cgtttgcttg tgtaacgatc atttcttgtc ttttttgcgg
    12481 agacgcaatg acgaattgtt cattcaattt ttcttcatat tcttttgcat gcgcaaatgt
    12541 cacatcttcg cctaagaaac gatgacctcg agaggctctg cctgttttga catccaagat
    12601 ttcgaatggg attacttcgt catctaacaa tgccacgatc cagtgaatag ggcggatata
    12661 ttcaaaatcg tattctgccc aatgcatcgt tacagggaaa gtcaaacttt tgatcacccc
    12721 tgctaagcct gtcaatactt cttttgcttc cttaccatga gtgtatttcg ttacatagac
    12781 atattccaca ccttttaatt ctctaaaagt gatagcgtct gtcgtcagac cttgtccgcg
    12841 gacaaatcct tccgctgctt tcgtccagtt tccatctgcg tccaaagcaa tttttttggc
    12901 tggtcctttg acttcttctt cactgtccgc ttgtcgttct ggaatatttg ttactttgat
    12961 tgctaaacgg cgcggtgtgg aaaatgtttc gatcgtatca taagtcaatt ggttttcatc
    13021 taaaaatttt gctgtttttt cttctaattg tttcatgctt ggtgtgacaa catgggcagg
    13081 catttcttcc agcccgattt caagtaataa gtcttttgcc atcttattca gcctccttca
    13141 cggtttctgg atttgctgtg tttttattca acaaagggaa tcctaatttt tcgcgttctg
    13201 ccacaaagat tttggctaca gcacgagcca tgttgcggat gcgagaaaga taaccggcac
    13261 gctctgttac ggatactgca ccgcgggcgt ctaataagtt gaatgtatgg ctacatttca
    13321 agatataatc atacgccgga tgaacaagat tttcatcgat acagcgttta gcttcttttt
    13381 caaaacgatc aaagttgctt agtagcaagt cttggtcgct tacttcaaaa gaatatttgg
    13441 aatgttcata ttctggttgt ttgaagatct cgccatattt cacgccatca gcccattcta
    13501 aatcatagac actttcaact tcttggatat aagaagcaag acgttctaat ccataagtga
    13561 tctcagaagt tactggttta caaggcagtc caccaacttg ctggaagtac gtgaattgtg
    13621 tgatttccat tccgtcaagc catacttccc aacccacacc ggcacagccc atggatgggt
    13681 tctcccagtt gtcttcaaca aaacggatgt cgtgttctaa tggatcgatt cctaaaaggc
    13741 gcaagctttc taaataaagc tcttgaatat tctcaggtga aggtttcatc accacttgga
    13801 attgatgatg ctgatacaaa cggtttgggt tttcaccata acgtccatca gctggtcggc
    13861 gagatggctc tacgtaagca gcattccatg gttctggtcc gattgcccgt aaaaaggtat
    13921 atgggctcat cgtacctgct cctttttctg tgtcataagc ttgcatcagc atgcagccgt
    13981 ttgatgacca aaatttttgt aatgttaaaa tcatatcttg gacggtcaat tttttactca
    14041 tctgttttgc ccctttcaat tttaaagcgt tgctgtcaaa taagaataca ggaaagaaaa
    14101 cgacaaaaaa tccctatgtc tgattacaga catagggacg aataattcgc ggttccaccc
    14161 tacttccgaa taaagattcg gcagcttcat ttatcgtact cgcaagtgcc tcttcaaaaa
    14221 tcgtttgggt ccggctccca ctctcccgaa ctcgctaaac cacgtgtttt ttactgatct
    14281 tggtcaatgt acgtattcat tttgcagttt tcttgtttct ataatagatc ctatcactga
    14341 acgatctatt taatctatgg ttaataatag acggaactcc ctgtgtttgt caaatggttt
    14401 tgattttttg ttgtatgttt tatttactat tcttcttttt caggtgtctc tttttctttt
    14461 tcagacatgt ctttctctga cgtctttaat acgtgtgccc aagacttcat ttgattgatg
    14521 aactttttgc ttttcaaatg gataccgaca tattcatcat acagctggtc gatgagttca
    14581 cggattgctt gtttggtttc ttctttcaga tggattccac taattttatc ataagaaata
    14641 gctgaaaaca tccgaacgaa ataaacagct cgaggatctg catgataacg gtgatcgtcc
    14701 atcgaccaat gtctttcgca tatcacacca ccatatttgg aagaatagtc aaattttcct
    14761 tttgtttccc cgcatacgac gcagtgtgtc cagattggtg caattccgaa gcgttgcagt
    14821 atttggactt caaaaatatt cgtgatgatt tctgcatcgt ctccttggtc catccgttgc
    14881 agtgcttgtt gcgtaaactg gaacaaatgc gggtcgtact gatgatcttc gattgccgca
    14941 tctactagat tcaataaata tgtgccatag ccgctcaaaa agatgtcttc ttggatgtgt
    15001 cgaaacgcat ggacatcttt gacactgctt aaaaatgaca aaccttcact tcgaaaattc
    15061 ccgatataaa ctgcttcagt aaaagggagc aaagccggag ctaagggctg attttgccga
    15121 tggatctctt tgatataaaa catcattttg ccagaagatt ctgtaaagat tttgactaat
    15181 ttatcttttt ctttgtaatt acggctagat aaaatcaagc cttttgattc tgcaagctgg
    15241 ctcatattct ccctcctaaa aaataaatct ggaaaagcag attctgccga tccagatcat
    15301 ttatgttgcg atcttccgca gcttttctca acacgcggat ttttattcag tttatttcta
    15361 tacctaattt acaagaagct cagaatatca gtattcgtct tttcggtagc cgaagtcttg
    15421 cagatagact tttttgtctc gccagtcttt ttgaactttg acccaaagtt ctagaaagac
    15481 tttgtctcct aaaagatttt cgatatcttt tcttgctttc gttcctactt gcttcagcat
    15541 ttttccgcct ttgccgataa tgatcccttt ttggctatct ctttcgacaa taatcgtagc
    15601 ctggatatgg attttatcat tctcgtttcg cttcatcgaa tcaacaacga ctgcgacaga
    15661 atgaggcact tcgtctcgtg ttaaaaacaa gactttctca cggatcaatt cagaaacgat
    15721 gaagtattca ggatgatcag tcacttgatc atctggaaaa tattgtgggc cctctggcat
    15781 ttcactgact aatacatcca tcaaacgttc gacgttgttt ccttctgtag cagagattgg
    15841 aatcacttgt gcaaaaggca tctgcgttga ataatcttcg atgattccca acaactcgtc
    15901 tggatggatt ttgtcgattt tattgataat cagataaaca ggagaatgca cgttttttag
    15961 acgttcaata ataaaatcgt ctccccgacc gcgtttttga tcagcactaa tcataaataa
    16021 gaccgcgtcc acttctttca acgcactgta agctgcttct accataaaat ctcctaaacg
    16081 atgctttggt ttatggattc ctggtgtatc aataaaaatc aactgagctt ctggtgtagt
    16141 gtatactcct tggattttat ttctagttgt ctgtgccttg tcactcatga tggcaatttt
    16201 ttgacccacg attcgattca ataatgtgga tttccctaca tttgggcggc cgacgattgc
    16261 gacaaaccct gatttatgtt ctggcatata tgttctcctt tttaattaaa caacaaatga
    16321 tatattttag ggacaaataa aatcaaccca acgatgacgg taaaacaagc ggtcactaaa
    16381 actgctccag ctgccatatc tttgattttt ttcccaatcg gatgaaaatg aaaatccgta
    16441 aacatgtcca cgacattttc aaaaacagta ttgatgatct ctactatcca tacgagaaaa
    16501 acagctaaaa gcagccataa ccattctatc cgatcaagtt ggaaaatcac gcccataaac
    16561 aaagcgagga ttccgaacgc aacatgtttc ctcatgttgc gttcttcctc aaaaacagtt
    16621 ttgactcctt ggacggcaaa ttccacggac atcaggaaat gtttgttttt ctctacgttt
    16681 ttatctttta agtccatagg catctaagat ctctttctgt aaaccaaaca tttctttctc
    16741 atcctctggt gtcatatggt catacccgtt gatatgcaag aatccatgaa ccgctaaaaa
    16801 gccaagttcc cgatcatacg aatggccgta agcttctgct tgctctgcag cacgttcagt
    16861 agagatcatg atgtctccca gtacacgagg caatggattc tctccatctt caaaaatcac
    16921 tgggatttcg tcttcgccct cttcttccaa ggcaaaactg atgacgtctg tcggtgcatc
    16981 ttttccacgg taatcacgat tgattacttg gattgctgca ttgtccatga aagtaacaga
    17041 catttctgtt tcttctggca actctaaata tgatgctgca aattgcagca agtcgtcgat
    17101 ttctttcatt ttctcttctg agacttggtt tgtttcatcc atgaaggtaa tatccattat
    17161 acatcgcttc tttctattct tcttgctatt cttcttgttc ttcttttatc ttctcaactt
    17221 ccgatttcat tttaggatat ttgatccgag aatggaaggt actgcttaaa ccgctgatca
    17281 atgatttttc tactttacgg atttctttta acgtcaatcc actgtcgtcc aattgtccat
    17341 cggaaatacg ctcttcaata agattatgga caaaactttc gattttttcg ctcgttggat
    17401 ggtccattgc ccgtactgcc gcttcacaac tgtcagctat attgacgatt cctgcttcac
    17461 ggctttgagg ttttggtcca ggataacgga attgctcttc cgtaacttcc gggttgcgtt
    17521 cctttgcctt aacgtagaaa taacgcatca gcgtggttcc atgatgctgt ctacaaatat
    17581 cgatcaccat ttgcggcata ttataatctt ccaatatttt ggcaccatct gtcacatgtc
    17641 caaagataat atgtttactg tcttctggca ataagaaatt gtgcggattt tccgctcctg
    17701 aaggcaagtt ctcaacgaaa aagctggcgt gtttgatctt gccgatatca tgataatagc
    17761 aagctactcg agttagtaaa gaacgtccac cgatttctgc tacagcatta gcacttagat
    17821 tggctaccat catgctatgg tgatacgtac ctggtgcttc ttccagcaac tgtttcaata
    17881 aaggatgatt gggattgctg agttcattta atacgatcac actatcatca ttgaccatca
    17941 attcaatata ggggtgcaga cctatcgtca gcaagaatga aaacagtgca cctgtgaatc
    18001 cgcagactag cgttaaccaa gtcgttgcat caccaaagct cattccttga tagacgatca
    18061 aaatgacatc catcatcaca ggaaaaataa tgacccacat ggcagcagat aatccttgtt
    18121 ctgagatccg tttacgtctg acaatcgttg ccatgagccc agagaaaaca taagacatca
    18181 agataatcgt tagaaagttc gtgccaatcg aatcataaaa aatgaaaagt gcggatactg
    18241 cttggaaaat accagccatg atccctgctc ttcgattcaa gaaaaagctt aaaacaagtg
    18301 gtgcaaaagc agcgggataa aacagcggga tataagtagc ttgttccgtt tggaataatt
    18361 ggaaaaattt catcagcact atgctgactg acatagctgt cacatagaaa agaacgtatt
    18421 ttgttctttc tcctgtattt tggaattgaa gtgaattata aatcagtacg ccgatttgaa
    18481 gcaaaacagc taaaaccatg gcaattagcg gaaaaatcga agttgtttga ttagtcagtc
    18541 ctagtaattc caattttttc atagctgaag catcaatttg attaccttca cgaacaatga
    18601 tttctccttg gaagatcatc actggttgta cagcttctcg tgcggattgt ttcaattctt
    18661 ctgtcttttt ctcattcaat acgtcattca ctacgatacc ttgatccact aagtagcgaa
    18721 tcatttgttg ttgtgctggt gtgacattca aatattggat ttgctcttct gcttcttgtt
    18781 taaaagcttc caattcactt tcacgaactt gtttgctcat ctgttcgtca atgagcttca
    18841 gactctcatc cctcacagta tgaatctcag tttcagtcat ttcaaatatc gtcgaataaa
    18901 aattgttcgg tagtttttga taaaacacaa cattttctgc attcacattt tcaaaatttt
    18961 tcttcagcgc ggcgatccgc tcttctgtag taggctttgg gatattttca tcttcctttg
    19021 ccttatcaat cttttcgtca taagatttgt tgatggcatc attcgtctta tcgatcaatt
    19081 caaatagttg cttgatccga ttgttctggt catctgcaag atctttttgg taagtgtatt
    19141 ctggagtgac tgcttctgct gccagttttc ttttttgttc ggtttcttcc gtgttctcga
    19201 tcgttttgtt cgcacggata ctttcttctg ctacttggcc ttctctgaaa tcattggcct
    19261 tttgtttgac actactaaat gtaacgccgc agataatgat aaaaaacaag aacagcagga
    19321 aaggcaatgc tgcctttcca agtttttctt gtagtttctg cagtgtttta ttcaacatgt
    19381 gcttatcctc ctaagcaaag cttattcttg atggagatct gccttttcat aagctttgat
    19441 gatttcggca actacagggt gccgaacaac gtcacttgct tcaaaattaa caaaatcgat
    19501 tttcttgatt tgctttaatg ttcgttctgc atgaacaaga ccacttgttg tccctcgagg
    19561 cagatcaatc tgactcgtat ctccattgac aatcattttt gattgataac caagacgtgt
    19621 caggaacatt ttcatctgag caactgtcgt gttctgtgct tcatccaaaa taacgaatgc
    19681 atcatccaat gtccgccccc gcatgtaagc taaaggagca atttcgatca cgcctcgttc
    19741 catcaatcgg ttcgtatgat ctaatccaaa aatttggtat aaagcatcat aaacaggacg
    19801 cagataagga tctacttttt cttttagatc cccgggaaga aagcctagat tttcccctgc
    19861 ttccacagca ggccgcgtca aaatgatttt ttgcacttca ccttttttca aggcagcgat
    19921 tgctaatact actgcaagaa aagtttttcc tgtcccggct ggtcctacgc caaatacgat
    19981 atcatgtttg gctaccgatt cgacatattt cttttggccg ctgtttttta cccgtatagg
    20041 ttttccatta cggtctttga cgatctcttc ctcatacatc tcaacaaaat aatctaatgt
    20101 gcctttttgc cccattttca gtgccgtcac gacatctggt gtacttacat ggattcctcg
    20161 tttgatcaat tcctgcaatg tccgaatgac tgaggcagcc aaggaaatct gggattgttc
    20221 gccaatgatc tgaatgattt ctccacgtgt atggatggtt gtttctgttg tttcttcaat
    20281 cagttttatg tgcttgtcgt gtgcacctaa aagcatgctg acatcatcag cagccgtcaa
    20341 tctaatctct aaagaactag actcttccgt caaaaataga catccccttt ttttcgattc
    20401 attctttcct attttaccat actcagaaga aactatatag caaataagaa aaggttctaa
    20461 aagagttgct taaccataag taccaagaaa cgttaaaaaa tagctgcttc cattctctaa
    20521 aggtctcgtt tgaataaaaa aagctgatca tgtaagctat gcataagata gaggacaagt
    20581 attccgtcta acttagcaag tattctctgg gtatttttct tgtttcaaaa aaagaaaaca
    20641 aaatatttga aatattcttg catttcaaaa aaaacaggtg tattataacg tcaacaaata
    20701 aatatacagc aaagcgtcga ggaaaacagt aggctattca gtgatttatt cccagtgagt
    20761 catgtaagtg agaaatgaca ataaatcgaa tagacgaaca tcatttccga gtcaatgtcg
    20821 aaaacgtaga tcattgtggc tgcctccatt atcaggccag cggataactt tatcgaaaag
    20881 cgtccgtgaa tgaagaagct tttttaagcg aaaaaaggtg gtaccgttct agaatctaga
    20941 gcccttttcc caagtggaaa agggcttttt ttatatttta aggtggtggt tttcatggaa
    21001 tctgatatta gtggtatttc tagtttctgc aacgtttatt ttgttactca aaatgataat
    21061 tattggagga aaaatttatg gaagaaacaa aaacttattc atggaagcaa ttattgatgg
    21121 tcagttcact tatcttcggg atgttcttcg ggtcaggtaa tctgatcttt cctgtccact
    21181 taggtcaaat ggctggatca aactggatca gcgctggtgt aggatttgcc atctcaggcg
    21241 ctcttttccc attattagct atcttagctg tagtagtgac aaaaagtgat ggtctgtatg
    21301 acttagctaa acctgtcgga aaatggtatg ctgccttgtt cttgatcctt gttcatttaa
    21361 cgatcggacc attctttggt acaccgcgga cagcagcaac tgcttttgaa atggctgcta
    21421 aacccttttt gcctgaaaaa tatgattcat taggtatgtt tttattctct gctcttttct
    21481 ttttacttgc ttatttcctt actgtacatc caacacgttt ggtcaaatat gtcggaaagt
    21541 atttaaactc cttgtttctt gcattattag ctatcatttt tgtcgtttct ttattccaac
    21601 ccatggggaa tctaaaccaa tctgctgcag attcttatca gacgaacgct ttgacaaatg
    21661 gcgttttaga aggatacaac acggttgatg cagtagcttt acttgcttta agtgtcactt
    21721 ttgtccatgc agttcgtgat cttggtttta aagataaaaa agtcacagaa ttaacagcaa
    21781 aagctggtag cctgagcgtt gcgttagaag tcatgattta ttttggtctg gtccttctcg
    21841 gtgcgatgag tttgaataag atgtctttat cagaaaatgg cggaactgca ctttctcaga
    21901 tcactaattt ttatttaggt aacttcggtg ctagcttctt aggggtaatg gtcacattag
    21961 gtgtcttcac taccgctatg ggactcgttg tttcttttgc acaagatttc cataaactgt
    22021 tccctaaagt cagttatatg acatggctgc ggttaacgac ttttgtctct tttgtcgttg
    22081 cgaatgccgg attggataat atcatccaat ggtctttacc agtattgatg cttttgtatc
    22141 cattgtcact tgcgttaatc ttgctttcct taacagcaaa attcttccaa aagagtccat
    22201 ttgtctatca agtaacgatg ttcttttctg ccgttccagc cgtattggat atgttggcaa
    22261 attcgcctgc attagtgagt cagcaaactg tcgtagcaag cttactagga ttttatcatc
    22321 attatgttcc ttttgctgca ttaggacttg gctgggtcgt cccaacgttg cttggttacg
    22381 ctggcagctt gctcttttat tatgtatatc gcgtatcagg atataaacaa gaagcaagtg
    22441 agttaccaga agaataacca aatcaaacac aatttaacat aagagatatt aaacaggaaa
    22501 aa
//
LOCUS       DACDEG010000038        22175 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.040, whole genome shotgun sequence.
ACCESSION   DACDEG010000038 DACDEG010000000
VERSION     DACDEG010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.040"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..413
                     /locus_tag="IV142_001477"
     CDS             <1..413
                     /locus_tag="IV142_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8485143.1"
                     /translation="TRHIRNLSNAKLEEEARKVSNYNKKMAAKYVNEEEFCFSKTKIY
                     KRNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLSQGGKDVIENCIALC
                     PNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
     gene            756..1012
                     /locus_tag="IV142_001478"
                     /pseudo
     CDS             756..1012
                     /locus_tag="IV142_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            1399..1626
                     /locus_tag="IV142_001479"
     CDS             1399..1626
                     /locus_tag="IV142_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP8485144.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            1642..2808
                     /locus_tag="IV142_001480"
     CDS             1642..2808
                     /locus_tag="IV142_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP8485145.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            3043..4086
                     /gene="hrcA"
                     /locus_tag="IV142_001481"
     CDS             3043..4086
                     /gene="hrcA"
                     /locus_tag="IV142_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP8485146.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            4180..4743
                     /gene="grpE"
                     /locus_tag="IV142_001482"
     CDS             4180..4743
                     /gene="grpE"
                     /locus_tag="IV142_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP8485147.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            4794..6623
                     /gene="dnaK"
                     /locus_tag="IV142_001483"
     CDS             4794..6623
                     /gene="dnaK"
                     /locus_tag="IV142_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP8485148.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            6774..7940
                     /gene="dnaJ"
                     /locus_tag="IV142_001484"
     CDS             6774..7940
                     /gene="dnaJ"
                     /locus_tag="IV142_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP8485149.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(8308..9045)
                     /locus_tag="IV142_001485"
     CDS             complement(8308..9045)
                     /locus_tag="IV142_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8485150.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(9047..10549)
                     /locus_tag="IV142_001486"
     CDS             complement(9047..10549)
                     /locus_tag="IV142_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8485151.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            10808..12802
                     /gene="uvrB"
                     /locus_tag="IV142_001487"
     CDS             10808..12802
                     /gene="uvrB"
                     /locus_tag="IV142_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP8485152.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            12812..15628
                     /gene="uvrA"
                     /locus_tag="IV142_001488"
     CDS             12812..15628
                     /gene="uvrA"
                     /locus_tag="IV142_001488"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP8485153.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            15891..17156
                     /locus_tag="IV142_001489"
     CDS             15891..17156
                     /locus_tag="IV142_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP8485154.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            17160..17891
                     /locus_tag="IV142_001490"
     CDS             17160..17891
                     /locus_tag="IV142_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP8485155.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            18026..18910
                     /gene="rapZ"
                     /locus_tag="IV142_001491"
     CDS             18026..18910
                     /gene="rapZ"
                     /locus_tag="IV142_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP8485156.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            18907..19905
                     /locus_tag="IV142_001492"
     CDS             18907..19905
                     /locus_tag="IV142_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP8485157.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            19939..20874
                     /gene="whiA"
                     /locus_tag="IV142_001493"
     CDS             19939..20874
                     /gene="whiA"
                     /locus_tag="IV142_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP8485158.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            21442..22098
                     /locus_tag="IV142_001494"
     CDS             21442..22098
                     /locus_tag="IV142_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485159.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
BASE COUNT         7350 a         3771 c         4804 g         6250 t
ORIGIN      
        1 aaactagaca tattcgtaat ttatctaatg ctaaattaga ggaagaagca agaaaagtta
       61 gtaattataa taaaaaaatg gctgcaaaat atgttaacga agaggagttt tgcttctcaa
      121 aaacaaaaat atataaacgt aatcccttcg tatctgaata tgttaaacga cttgcaaaag
      181 gtatttgtca actttgccaa gaaaaagggc catttataaa agacggtgta ccttatttac
      241 attgtcatca tatagaatat ttgtcgcaag gtggaaaaga tgtgattgaa aactgtatcg
      301 cgttatgtcc caattgtcat gctagaattc atgaacttga gttacctgac gataaagaaa
      361 aattgctgaa gatcgttgaa aaaagagaaa atagcttttc taaaataccg taatttattt
      421 ttataggaca aataagctaa tgtgagctat gccttctata taagactaag ttctattata
      481 agtaacgaag taattctcgt tccttcctgt agtaatattt tttaaataac ctataggctt
      541 cttcaaaagt acatcctcca gtaaattcta agtactggag gattcttttt ctagtgttgc
      601 tttactcatg aaaaagtagt tggctacttc atcaaaattt ctgaactttt ttaatgtata
      661 ataaagagct cgatcattta ctacaaggat gctagctcct acattttcac agttttttag
      721 caagtaaatt tttctaaaat cacatcagga aatgcatgag attctttaag agaggcaatt
      781 atagggctat gattacggtt cgtgtaagtg acgccaaaaa agaatggctt tcttatatgg
      841 ctgaatttta tgggattact ttatctgaac ttctaaaaaa ctattcgatg gaacaactag
      901 aagatgaata caatcgtcaa acggctgaaa ttgcccataa acattggata agaagtggga
      961 aaacaacagt attcatggaa gaatccttaa tgaatttggt gggctggaat gacctgccgt
     1021 ttaaaattca cgcccgaaat tcagaagcag ctctgattgg caaatacgtg tattggtaga
     1081 gatagaagat aatcaattcg ttgttatcgc tattcaagta gggcaccgaa gaaatgtcta
     1141 tgattaagag actgtttatt tttacaaaaa aataataatt ggaatctagt taaaactctt
     1201 gaagtgagag atttgactag atttttttat gaataaaata aggacaaaat tgtttaggaa
     1261 gaatcatgag tgtaaaaaaa tcatccaaga agtcgattct tggatttttt agtggatttg
     1321 ttcgccaaat acgtttagta tgcgtatatg agaggttgta agcaaagcgt tgtggtctga
     1381 gcacttaagg aggaatctgt gaaaatagct tttcatattt ttgcagattt tgacttaatg
     1441 ccgaaggtca gctttgcgat taccgtttat cgagaggctg taaaagcacc gtgtagctcc
     1501 gatgacaata agcaagcatt tttagagcta ggtttccttg attctaaata tgcggcttat
     1561 ttcaatgagt tggtgcttga acgcgatttt aagaaggtcg aaaacaggca cgacttttcg
     1621 acgtaaggag aaagaatttc aatgatagaa acaataaatc ccgcagaaaa caaggtttct
     1681 gcgtacattc atatcccatt ttgcgagcat atctgctatt actgtgactt taataaagtt
     1741 tttttagaag ggcagccagt agatgagtat gtggaaatgc tgctgaatga aatgagatta
     1801 atgctggaaa gtcatcctac agcgaatttg gaaacacttt atgtaggagg cggaacacca
     1861 acttctttat ctgctaaaca acttgatcga ctattatcag gtgctagaga gatcctgcct
     1921 tttcaaaatg gtaatgagtt tactgtagaa gcgaatcctg gtgatttgac ccgtgaaaaa
     1981 cttcaagtga tgaaaaacta tggcgtcaat cgtttatcca tgggagtgca gacatttgac
     2041 aatcgcttat tgaaaaaaat tggacgtaaa catactgcag aagatgtgta tcaaacaatg
     2101 caatttttag aagcagaaaa ttttaccaat gtaagcatcg atttgattta tgcattaccg
     2161 ggacaaacac tagaaggata tcgggatact ttggaaaaag ctttggcttt ggatctgcct
     2221 cactattctt tgtactcatt gatcctcgaa aacaaaacaa tgttcatgaa ctgggtacga
     2281 caaggaagac tcgaactgcc tgatcaggaa acggaaacca gaatgtttga agagacgatt
     2341 gaagcaatgg aaaagcatgg acgtcaccaa tatgaaataa gtaactttgg gctctccgga
     2401 catgaatctc aacataacct catgtactgg aacaatgacc actattttgg gtttggagct
     2461 ggtgctagcg gttatcttgg agatattcgt taccgcaaca aaggaccaat ccaacactat
     2521 ttacgcccat tacgagcaaa tgaacgcccg gtattggaag aagaaaaatt gacacgaaaa
     2581 aatcaaatag aagaagaaat gttcttagga ctaagaaaaa aaacagggat tactaaaaaa
     2641 catttctatg atcgctatca aaaaacaatt gaatcggtgt acggtgatgt gctaagggaa
     2701 ttagagcaag aagggttact tgttaatgaa gaagaccgtg tttatttgac accaagaggg
     2761 acttttttgg ggaacaatgt gtttgagcgc tttttatttg aaaaatagtt tgaagaatat
     2821 ttgtttttta tcgaaacttg aaaagaaaaa gtttagaaag tttgacaatg aggtattgaa
     2881 gtattgatat aactggattt acatcaatta tttagaatta gcactcttgt gtttagagtg
     2941 ctaaaaaatg gagtctttct cttgacagga gaagggcttc ttgctatatt tataagtgaa
     3001 ttagcactta gaatatatga gtgctaatga gaggtgagga agatgttaac aaagcgacaa
     3061 aacgacattt tacgtctgat catccaacac tatacgagta gtggcgtgcc cgttggttcc
     3121 aaaacattaa tgtctgaggg agtcaaggcc agctcagcaa cgattcgcaa tgatatgaaa
     3181 gcattagaag atgaaggatt gctgttgaag actcactctt cttctgggcg gattccatct
     3241 gctttaggct atcgttatta cgtggatcat ttgttaaaac ctgctcgcgt agcagaagat
     3301 gatttgctgc tgattcgtca gtctctaggc agagaatttc atgaaatcaa tgagattatt
     3361 aagcaatctg ctgagatatt atctgagctg acaagctata cgactttttc attgggacct
     3421 gaaataaaag accgccgact gactggcttt cgtatcgtac ccttgaataa tcgacagatt
     3481 attgctatcg tcgtaacaga tatggggaat gtagaaagcc aagtgttcac tcttcccgaa
     3541 tatatgggaa gtcaggaact agaaaaaatg gtacagattg taaacgaccg tctagtcgga
     3601 gatcctctga tgacggtgta ccataaactt cgtacggaga ttccgatgat tcttcataaa
     3661 tatttccaga cgacagaagg gatgtctcag ttatttgaca ccgttcttgg tcatgttttt
     3721 gaagaaaagg tttatgtcag tggacaaatg aatctcttgg attatgagcc ccgtcaagat
     3781 cttgatcagt tcaaatcgat gttttctttt atgaaagact cagacggact tacacaaatg
     3841 atcactccga tggatagcaa tattcatatc aagattggtg ctgaattagg caatgattta
     3901 ttgcagaata tgagtatgat tcaagcaagt tacgagatag atggtcatgg aagaggtaca
     3961 atagccttgt tagggcctac tagtatgcct tattctcgga tgttcggtct agtcgatgtt
     4021 tatcgaagag aactagcagc taaacttgct gattactacc gctcgcttga cttgtcaaac
     4081 tcgtaatgtg aataacgaag ctatttgcag ctgatcgttc tctctttctt agtaagagca
     4141 atcaaacagc tgactgaata atgaaaggaa gactgtatcg tgaaagaaaa tcaagaagaa
     4201 ctagataaag aaatgacaga tgctcagcca gaaccggaaa tcgatgtcga agctgcagaa
     4261 gattcgggta tttcagaagc tgaagcggaa gaattcgaaa cagcgaaatt aaaagctgag
     4321 ttagaagaaa tggaagataa atacttgcgg gctcgtgctg aaatcgcaaa tatggcaaat
     4381 cgaggaaaaa atgagcgtga gcaattacaa aaataccgtt cacaagattt agccaaaaaa
     4441 ttacttccat caattgacaa cctggaacgt gcattagcca cagaagtgtc agatgatcaa
     4501 ggggcaggtt tgaaaaaagg cgtggaaatg gtcttggaaa gtttgcgtaa cgcactggaa
     4561 gaagaaggaa ttgaaaaaat ccctgctaaa ggagaagcct ttgatcctaa tcttcatcaa
     4621 gcagtacaaa ctgttccagc gactgaagat acaccagccg atacgattgt agaagtacta
     4681 caagaaggct acaaattaca tgatcgcgtg ttaagaccaa ctatggtcat tgttgcacaa
     4741 taattgtgcg gatataaaac ttacactttc aattaaagaa ggagacgtta attatgagta
     4801 aaattatcgg tattgactta ggtacgacta actctgcagt agcagtattg gaaggaaacg
     4861 aagcaaaaat catcgctaac ccagaaggaa accgtacaac accttctgtt gtatcattca
     4921 aaaatggaga aatccaagta ggggaagttg caaaacgtca agctgtgaca aacccaaata
     4981 cgatttcatc aatcaaacgc catatcggtg aagcaggtta caaagtagat gtagaaggta
     5041 aatcatatac accacaagaa atctcagcaa tgatccttca atatatcaaa ggattcgcag
     5101 aagactattt aggtgaaaaa gtcgaaaaag cagttatcac tgtgccagct tactttaatg
     5161 atgcacaacg tcaagcaaca aaagatgctg gtaagatcgc tggtctagaa gtagaacgta
     5221 tcgtcaatga accaactgca gctgctcttg cctatggttt agataaaaca gatcgcgacg
     5281 aaaaaatctt agtattcgac cttggtggcg gtacttttga cgtatctatc cttgaactag
     5341 gagacggtgt ctttgatgta ttgtcaacag ctggcgacaa ccatctaggt ggggacgact
     5401 ttgataacaa aatcatcgac tacatggtag cagaattcaa aaaagaaaac agcattgact
     5461 tgtctcaaga caaaatggct ttacaacgct tgaaagacgc tgctgaaaaa gcgaaaaaag
     5521 atttatctgg cgtatcaagc acacaaatca gcttgccatt tatcacagca ggagaatctg
     5581 gaccattgca tttggaaatg acattgacac gtgcaaaatt tgatgaatta acagctgact
     5641 tagtagaacg tacaaaagta ccagtacgtc aagcgttaaa agatgcaggt ctgtcacaat
     5701 cagaaatcga cgaagttatt ttagtcggtg gatctactcg tatccctgca gttgtagaag
     5761 cagtacgtaa agaaacagga aaagaaccaa acaaatcagt taaccctgat gaagtagttg
     5821 ctatgggtgc tgctatccaa ggtggcgtga tcactggtga tgtgaaagat gttgttttac
     5881 ttgacgttac accattgtca ttaggtatcg aaacaatggg tggagtgttc actaaattga
     5941 ttgaccgcaa tacaacgatt ccaacaagta aatcacaagt attctcaact gcggcagata
     6001 atcaaccagc agtagacatc catgtattac aaggtgaacg cccaatggct gctgacaaca
     6061 aaactctagg aagattccaa ttgacagata ttccagcagc tcctcgtggt attccacaaa
     6121 tcgaagtaac attcgatatt gataaaaacg ggattgtaaa tgtatctgcg aaagatctag
     6181 gtacacaaaa agaacaaaaa atcacgatca aatcttcttc aggtctaaca gacgaagaaa
     6241 tcgaacgtat ggtgaaagat gcagaagcaa acgctgaagc agataaagct cgtaaagagg
     6301 aagttgacct acgtaatgat gtagacgcat tgctattctc tgtggacaaa acattgaaag
     6361 aattagaagg caaagtggac gaagaagaag tgaaaaaagc agaaacagct cgtgacgaat
     6421 taaaagccgc tgttgaagca aacaacatcg aggaaatgaa aactaaacgc gatgcattga
     6481 acgaaatcgt tcaaaacttg actgttaaac tatacgaaca agctgcacaa caacaagctc
     6541 aagaaaatcc agaagcagct caaggcggag cagatgatgt cgttgatgct gattttgaag
     6601 aagtcgatgg cgacgataaa taatcagttc actgaatttc gagagaaggc caaagttgct
     6661 actttggctt tttcttttga aaaatagttt gaagtatcgg tttttgtttc aaacaaaata
     6721 tgatatgata acaagcgacg tgaaaatgat gagaaccaat aggaggaaag cctatggcaa
     6781 caaaacgtga ttattatgaa gtcttagggc tctcaaaagg agcatcagaa gatgaaataa
     6841 agaaagctta tcgtaagctt tcgaaaaaat accatcctga catcaataaa gaacctgatg
     6901 cggaagaaaa attcaaagaa gtatcagaag catatgagat tttaagtgac ccgcaaaaac
     6961 gcgctgcata cgaccaatac gggcatgcag gcacagatcc aaattacggt gctggtggag
     7021 ctggtggcgg atttggtgga ttcggcggct tctctggtgg cggcttcggt ggttttgaag
     7081 atatcttcga ctctttcttt ggaggaggcg gtcggtcagt cgatccaaat gcgcctcgtc
     7141 aaggtgctga tttgcaatat acggtcgact tgtcttttga agaagcgatt tttggtgtag
     7201 aaaaagaaat caagtataat cgggaagaaa tctgtcatac ttgtggtgga aacggtgcaa
     7261 aaccaggcac acaaccaact acttgtcata aatgtcatgg tgcaggtacg atcaatgtag
     7321 aacgccaaac gccacttgga cgagtgatga gccgtcaaac ttgtgatgtt tgtcatggca
     7381 cagggaaaga aatcaaagaa ccttgtccaa cctgtcatgg ctcaggacat gagaagaaag
     7441 cacattcagt taaagtgaat gtaccagctg gtgtagaaga aggacaacaa atgcgtttag
     7501 ctggtcaagg agaagctgga gaaaacggcg gaccatttgg tgatttgtat gtggtttttc
     7561 gtgtagaaga aagtgatatc tttgatcgtg aaggttctga aatttactat gaattgcctt
     7621 taagttttgt acaagcagct ttgggggatg aagtccaagt accaactgtc catggagatg
     7681 tgaaattgaa gatccctgct ggaacacaga caggtacgaa cttccgttta cgtggaaaag
     7741 gcgctccgaa gttacgtggt ggtgcaacag gcgaccaaca tgtcaaagta aaactgatca
     7801 caccgaaaaa tctaaatgaa caacaaagag aagcattacg tgcattcgct gaagctggcg
     7861 gcatcaaagt tgaagaacag caagaagacg gtttcttcga taagtttaaa gatgcttttg
     7921 gcgggaaaaa gcggaagtag tttttctagt aaaaatataa agtagacttg cccaaatttt
     7981 ctatttctag taaaaacgct aagtcattaa taattaaaaa ccagacgaac aatcatacgt
     8041 tcgcctggtt tttttgtagt tgtcatgttt gtaccaacaa aaaagccagc tttagaataa
     8101 gataatcatc atagtttctg aaggtggtat cgactatgta cttgatatcg tcaatgaacc
     8161 gatggtactt tcttatgcga tggaacaggt agagcgattg cttgtttgtg caggagaaca
     8221 agcgatgcgg ctggtacagt taagtgaaaa aacaagaaac taaaaatgcc cggtcgtcat
     8281 gaagcgaccg ggcatttttg gtttttatta aatatttaat actttattca agaaatcttt
     8341 tgtccgttca ttttgcggat tttcaaagat ttgttgaggc gttccgtctt ctaaaaagtt
     8401 tccgtcgtca atgaacatga cacgattggc cacttcttta gcgaagccca tttcgtgcgt
     8461 aacgataacc atcgtcatac cttgttctgc taactttttc atgacgttca agacatcacc
     8521 gaccatttcg gggtcgagtg cagatgtagg ctcatcaaaa agcatgatgt cagggttcat
     8581 cgctagagca cgagcaatag ccacacgctg cttttgtcca ccggaaagca tttcaggata
     8641 catatctttt ttatcttcta gaccaactgt ctgcagcaat ttgattgctt ttttctctgc
     8701 ttcttctcta gattctttct tcaggtccat cggtgctaaa atgatgtttt ccaaaataga
     8761 aagatgaggg aataggttaa agtgctggaa taccattccg atatgttgtc tcaccaagtt
     8821 gatatctgtg cttttatcca tcaagtgggc gccgtcaata ataatttcac cgtcagaagg
     8881 ttcttctaaa cgattcaagc aacgcaaaaa tgtacttttt cctgaacctg aagggccaat
     8941 gatacagacg acatctcctt cgttgatgga cacattgatg tcgtttaata ctgtgttatc
     9001 gccgtatttt ttgactaaat gctcaactaa gattttttct gccatcttac ttcaccttct
     9061 tttctaaaac ttttgctaat tttgtcaatg ctgtgatcaa aatcaagtac atgatcgcaa
     9121 tgatcaagta aacatacgta ctttgtgttg tacgtgcaac aatgatctta ccagtttgca
     9181 gcaattcgac tacaccgatt gcggaaatga tcgttgtgtc ttttaatgag ataacgaact
     9241 gattgacgaa tgatgggatc atgatgcgta tcgcttgcgg caaaatgatt ttttgcattg
     9301 tccgattata agaaagacct aaacttctgg aagcttccat ttgtccgaca ggaacggcgt
     9361 tgatcccgcc tcgaacaatc tctgcgatat aagcacttgc gttaagagtc aaggtgataa
     9421 caccagcaag gaagtcagga atcgtaaatc caacgatacc aggcaaccca aagaaaataa
     9481 agaacgcaag aaccatcatt ggaattcctc ggatgatatc gacataaata ctagcaattg
     9541 tgcgaagagc gcgtattggt gctacactga ataatccaaa aatcacaccg attactatag
     9601 ccaaagcaaa ggagatcaaa gttaggagaa ttgttctcca aagtcctttc aacaatgctt
     9661 gatagttgtt ttggataagt ccaagaaatg ttgattcatc agcagtggaa gactcactgg
     9721 aagtttctga accatccgct acatattctt taataatctt atcgtattcg cctgttcttt
     9781 ttaattcttt caatccttca ttgaacatat taagaagctc aggattttgt ccttttttaa
     9841 cggcaaatgc atattttccg ccagattctt ttttgatcgg tgtttccaat tcttgtcctt
     9901 gtttgatcgc ataaccgatt accggataat catccatcat tgcatcgatt gcgccaactt
     9961 ttaacccgtc gtacaattga tcagcagcat caaaatattt gatagaatag ccatattcag
    10021 attcatggtc gcttaaaaat tcagcacttt cggtaccaac ttttgctccg actgtttttc
    10081 ctttcaaatc ttcataagac ttgatcgaag tatctccttt tttgacagct aattggatcc
    10141 cgctatcaaa gtaagaatca gaaaaatcat agcttttctt tcgttcatct gttacagtca
    10201 ttccggcaat cattccgtct gcttgtcctg attccaaagc ttgcaaagca gcagagaaac
    10261 cacggtagtc cattcggatg ttaaatcctt gcagtttggc gatgttttcc atcaatttta
    10321 catcgatgcc ttcgtactcg ccctttgcgt tttgaaattc aaatggagca aaagcagagt
    10381 cactggcaat tacgtatacg tcttttttgg gctctatttt ttgcattgtc tcgcttgtgc
    10441 ttgactctgc cgttgtagtg ttgtcagctt gtgcaacatg tccaaacgca agtgtcacaa
    10501 gtcctgtcat taagaaaaca aatgagtgaa ataaatgctt tcttttcata atcaacctcc
    10561 taaatttcca atattttatt attataggcg aaaagtgctg aatcagacaa gactgtaagc
    10621 tcgaaaaaat gaaaaaaatg cgagtttcta acattttatg ttataaaacg taacatgcat
    10681 aataagataa aaaagtggcg aaagtatgtt cggtgtggta taatttttta gtaaggaatg
    10741 cctttgcttt ccttttcttt ttattggtcg taaaatgaaa aggaatgaat gaaaaggagg
    10801 acgaagaatg attgagagag atacctcccg acactttgag cttgtctcga aataccaacc
    10861 ggctggtgat cagcctgaag cgattaacca attagtggat ggcgtagtcg gcgggaaaaa
    10921 agcacaaatc ttattaggtg cgactggaac agggaaaaca tatacgattt ccaatcttat
    10981 tgagaaagtg aataaaccaa cactaattat tgcccataac aaaacattgg ctggtcagct
    11041 gtacggggaa ttcaaggaat ttttccctaa caatgctgtt gaatattttg tttcttatta
    11101 tgattattat cagcctgaag cttatgtgcc ttcaagcgat acgtatatcg aaaaagattc
    11161 aagtgtaaat gatgagatcg acaaattacg acattcggct actagttcat tattagaaag
    11221 aaatgatgtg atcgtcattg catccgtttc atgtattttt ggattaggtt cgccttttga
    11281 gtaccaaaaa caagtagttt cgattcgaca gggagcagaa ctagatcgca atcagttgat
    11341 tcgtgactta gtcagtatcc aattcgaaag aaatgatata gattttcagc gcggtcgttt
    11401 tcgagtgcgc ggtgatgtcg tggagatttt tccggcttca agagatgaac gtgccttacg
    11461 tgtggagttt tttggagatg aggtcgaacg tatccgagaa gtcaatgcac tgacaggtga
    11521 agttttagga gaaacggaac acgtggcaat cttcccggcg actcactttg tgacgaatga
    11581 tgaacatatg gagcatgccg tagcgaatat caaagcggaa ttagaacagc gtttaactgt
    11641 cttgcgaaat gaaaacaaac ttctggaagc tcaacgttta gaacagcgaa ccaattacga
    11701 tattgaaatg atgttagaaa tgggctacac gtcgggaatt gaaaattatt ctcggcatat
    11761 ggacggacgt aaagaaggtg aaccgcctta tacgctattg gatttctttc ctgaagattt
    11821 tttgatcgtg gcggacgaat cgcacgtaac catgcctcaa gtcagaggaa tgtataatgg
    11881 tgaccgagcg agaaaacaga tgctagtaga ttacggtttc cgtttgccat ctgctttaga
    11941 caatcggcca cttcggttag aagagtttga aaaacatgtc aatcagatca tttatgtctc
    12001 tgcgacgcca ggaccatatg aacatgaaca aacagatacc gttatccaac aaatcatccg
    12061 tccgacaggg ctgcttgacc cagtgatcga agttcgtcca atcatgggac aaatcgatga
    12121 tctagtaggc gaaataaatg aacgagtcga aaaagaccag cgggtgtttg taacgacatt
    12181 gacgaaaaaa atggcagaag acctcacaga ttatttcaaa gaattaggga tcaaagtcaa
    12241 atatttgcat agtgatatca aaacgctgga acgaacagaa atcattcgag atcttcgatt
    12301 aggtgaattt gatgtgttag ttggaatcaa tttgcttcgt gaaggattag acgtacctga
    12361 agtttccctt gttgctatct tggatgcgga taaagaaggc tttttacgta gtgaacgttc
    12421 cttagtccaa acgattggac gagctgctcg gaatgaagaa ggtaaagtaa tcatgtacgc
    12481 agacaaagta acagattcta tgcgtctagc gatggatgaa acatcaagac gccgtacgat
    12541 tcagcagaag tacaatgaag aacatgggat cgttccaaaa acgatcatta aagaaatccg
    12601 agatttgatt tctattacga aagaatcgga agatgataca aaagaagcgg tacaagtctc
    12661 gtatgaagaa atgacgaaag aagaaaaaga tacgttattg atgaaattag aaaaagaaat
    12721 gaaaaatgcg gcgaaagcat tggattttga aacagccgct aatgtgcgag atatgatttt
    12781 agaattaaaa gcttcaaagt aggaggacaa catggctaac gataaaatta cgattcacgg
    12841 tgcacgtgcc cataacttga aaaatataga tgtaacgatt ccacgagata aattcgtggt
    12901 cgttacaggt ttgtccggtt ctggaaaaag ttccctagca tttgatacgc tgtacgctga
    12961 agggcagcgg agatacgtag agagcctttc tgcttatgct cgtcagtttt taggacaaat
    13021 ggataagccg gacgtagata gcatcgacgg attgagtccg gcgatttcta ttgatcagaa
    13081 aacaacaagt aaaaatcctc ggtcgacagt aggtactgtg acggaaatca atgactattt
    13141 acgcttgttg tttgcacgtg taggacatcc gatttgtccc aacgatcata tcgagattac
    13201 cagtcaatca gtagatcaaa tggcagatca agtaatggct ttgcctgagc gcacaaagat
    13261 ccaagtgttg gcaccgatcg tggtaaagaa aaaaggacaa cataaaaaaa tattcgaacg
    13321 aatccaaaaa gagggatacg tccgcgttcg agtcgatggt gagatctatg atttaagtga
    13381 agtgcctgag ctggagaaaa acaaaaaaca tgatatcgcg atcgtcatcg accggatcat
    13441 tgtgaaagaa gggattcgtt ctcgtctatt tgattcttta gaggcggctc tgcgattggc
    13501 agacggctat gctctggttg acgtgatcgg tgaagaagaa atgttgttta gcgaacacta
    13561 tgcctgtcct tattgcggat ttactgttgg cgaactagaa ccaagactgt tctcattcaa
    13621 tgcgcctttt ggagcttgtc cagactgtga tggtttaggt gtcaaattag aagtcgatca
    13681 agatttagtg attccagata gaacgaagac acttagtcaa ggtgctatcg ttccatggaa
    13741 tccaatcagc tctcagtatt atcctcaaat gttagcgcag gcttgtgaaa gtttcggtat
    13801 cgatttagat acaccattcg aagaattacc ggaagagcat caggatatca tcttgaatgg
    13861 gacagatgat cttttccatt tccattatga aaatgatttt ggtggcgttc gcgatgtaga
    13921 aacgactttc gaaggggtat tgaagaatat cgagcgtcgt tatcatgata cgaacagtga
    13981 tttcacgcgt gagcaaatgc gtctttatat gacagaatta acttgtcaga catgtaaagg
    14041 ttatcgattg aatccccaag ctctatctgt tcaaatcaat ggaacgaata tcgggcaaac
    14101 aaatgagttg tctattcaaa aagcagtaga tttcttctca aacttaacgt tatctgaaca
    14161 agagaaagta attgccaaac cgattttaaa agaagtaaat gacagacttt cattcttgga
    14221 aaatgtggga ctagactatc taactttgag tcgcgcttcc ggaacattat ctgggggaga
    14281 agcgcagcga atcagattgg ctacacaaat tggatcgaat ctttctggag ttttgtatat
    14341 tttagatgaa ccatcgatcg gattgcatca acgcgacaac gatcgactga ttggctcatt
    14401 aaagaaaatg cgtgatcttg gcaacaccct cgttgtagtc gaacatgatg aagatacgat
    14461 gtgggcagca gattatctga ttgacgtcgg acctggggcc ggagaacaag gcggacaaat
    14521 cgtggctgca ggcactcctg aagaagtagc taatgatgaa aattctttga cagggaaata
    14581 tctttcaggg aaaaaatcaa tccctgtacc aaaagaacgc cgtaaaggta atgggaaagc
    14641 aatcaagatc actggtgctt cagaaaacaa tctgaaaaat attagtgttg aatttccatt
    14701 aggagaattt gtagcagtca caggtgtgtc tggttctgga aagagtacat tggtcaatag
    14761 tatcttaaag aaatcgttag cgcaaaaatt aaataagaat tctgctaagc caggtaaatt
    14821 caagacaatt tccggctacg aaagtatcga aaagatcatc gatatcgatc aaagcccaat
    14881 cggccggacg ccgagaagta atccagcgac ttatacaagt gtatttgatg atatccgtgg
    14941 gttatttgct caaacgaacg aggcaaaaat gcggggttat aagaaagggc gttttagttt
    15001 caacgtaaaa ggcggtcgtt gtgaagcttg tcgcggggat ggaattatta agatcgaaat
    15061 gcactttttg cctgatgtct atgttccttg tgaagtatgt catggcaaac gatataactc
    15121 tgaaacatta gaagtgcatt acaaaggaaa aagcattgct gatattttgg aaatgacagt
    15181 agaagatgct gtagaattct tcaagcacat tccaaagatt catcgcaaac tgcaaacgat
    15241 tgttgatgtt ggcttaggtt atgtgacgat ggggcaacca gcaacgacat tgtccggtgg
    15301 tgaggcacaa cggatgaaac ttgccagtga attgcacaaa atctctaatg gaaagaattt
    15361 ctatatacta gatgaaccaa cgacaggact tcatagcgat gacatcgccc gcttgttgca
    15421 tgtattacaa agattagtag atgctggtaa cacagtttta gtgattgaac acaatctaga
    15481 tgtaatcaaa acagcagatt atatcattga tttaggacca gaaggtggag aaggtggagg
    15541 aacgatcctt gcgactggaa caccagaaga aatcattaac gtaaaagaaa gttatacagg
    15601 tcactatttg aaaaaaataa tggtataaag aggatagata gctgagatag ttaaaaaaac
    15661 ataaaaaaat gaatttttgc ttgttttatt aatatcagtg ttctagaatc attacatggt
    15721 agttctttct acactgattg gtatagacaa aaacttccta taagttcaat ccgttcaaaa
    15781 aagaattgag caatcggagg agtttccttt gattgctttt ttctttatgg tatgatattc
    15841 cttgtgtagt tggaaaagaa aaacaagaga ttatgagtaa gtgagagata atgatgaaca
    15901 gtattgaaaa tgaagaattt atccctatat tattaggaag cgacatgaac gtttatggta
    15961 tggcacgttc ttttaatgaa gcatacggca aaatctgcca agcatatgct tcggatcagt
    16021 tagcccctac ccgctacagt aaaattgtca atgtagaagt tattccagga tttgataaag
    16081 accctgtgtt tatcgagacg atgcttcgtt tggcaaaaga aagatattct gataagagta
    16141 aaaaatatct tttgattgct tgtggtgatg gatatgcaga attgatctcc caacataaac
    16201 aagagctgtc agaatatttt atctgtccat atattgatta ttctttattt gaaagactga
    16261 tcaataaagt aagcttttat gaagtttgtg aagaatatga tcttccgtat cctaaaacat
    16321 tgatcgtcag agaagaaatg ttagtaaatg gacatttgga acaggaattg ccttttgaat
    16381 tcccagttgc tttgaaacct gcaaacagtg tggagtatct ttctgtccaa tttgaaggaa
    16441 gaaaaaaagc ttttatttta gagactagag aagagttcga tttaatttta ggccggatat
    16501 acgaagccgg ttataaaagt gaaatgattg tacaagattt tatcccgggt gatgatagta
    16561 atatgcgtgt gctaaatgcg tatgtagatg aagatcatca agttcggatg atgtgtcttg
    16621 gtcatccact gttagaagat cctacaccag catcgattgg taattacgtt gtgatcatgc
    16681 cagattataa cgaaaaaatc tatcaaacca ttaaggcatt tttggaaaaa atcgaatata
    16741 caggctttgc caatttcgat atgaagtatg atcctagaga tggagaatat aaattatttg
    16801 aaatcaattt gagacaaggt cgtagcagtt tctttgtgac attgaacggc ttgaatctgg
    16861 ctcgtttcgt cacagaagat cgagtgttta acaaaccctt tgttgaaacg acctatggaa
    16921 cgaatcaatc agataaagca agattatgga tgggtgtgcc caaaaaaatc tttttagaat
    16981 atgctcggga aaatgaagac aaaaaactag ctgagcaaat gatcaaagaa aatcgttacg
    17041 gtactactgt gttttatgaa aaagaccggt caatcaagcg ctggttgttg atgaaatata
    17101 tgttccataa ctatattcca agattcaaaa agtattttca tgtgaaagaa ggctagttca
    17161 tggagaattt tttcagtatt ttaggcggaa tgggcacgat ggcgacagaa agttttgttc
    17221 gcttgatcaa ccaccgaacg aaagctacaa aagatcaaga atatttgaat tatgtcttat
    17281 tcaatcatgc aacagttcct gatcgtacag cttatatttt agatcgatca gaagaaaatc
    17341 caatgccgtt tctgctggat gatattgaga aacaaaattt attgcagccg aacttcattg
    17401 tgttgacttg taacaccgca cactactttt ttgaagaatt acaagcggca acggatattc
    17461 ctatccttca tatgccaaga gaagcagcaa atgaacttgt ccgtcagcat acgacaggga
    17521 gagtagcgat tctaggaaca gaaggaagta tgaaagctgg aatctatgaa cgcgaagtca
    17581 aaaatcttgg attcgaaaca gtgattcctg atactgttct tcaagaaaaa atcaattatc
    17641 tgatctatca tgagatcaag gaatcggatt atttgaatca agagctatat tacgaaatct
    17701 tagaagaagc ggtagagcgc ctaaactgtg aaaaagttat cttaggttgc acagaattgt
    17761 cattgatgca tgaatttgct gaagataacc attatccagt gatcgatgcc caatctattt
    17821 tagcagatcg gacgattgaa cgagcgctag ctgaaagaag caaggcattg gatacagcat
    17881 cggaaaagta aaaaaattgg atttggggca aatatcctaa aagatatttg gtccaaatct
    17941 ttttgtaaaa gaaaaagaaa actggtaatg atacgcccgg aatgatataa ttttactatg
    18001 cacaatgtga gaggagttcg taattatgac agataatttg caattagtca tcataacagg
    18061 tatgagcggt gcagggaaaa cagtagctat ccaaagtttt gaagatatgg gctatttttg
    18121 tatcgataat atgccgcctc gattgattcc aaaattctgg gaattaatca aagaatcagg
    18181 caaagtcaca aaaatggctt taggagtgga tttacgttct cgttccttct ttgaagaaat
    18241 ccaaaacatg ctgatcgaaa tcgaaaatac gaacttcatc gacacttcta tcttattttt
    18301 agatgcttca gacgaagaac tagtggctcg ctacaaagaa acaagacgag cacatccaat
    18361 ggcgatggac ggtttgatca ctgaagggat acgtaaagaa cgggcgatac tagacgactt
    18421 gaaagcccaa gcttccgtga tcattgatac gacgacattg acaccacgac aattacgtga
    18481 aaaaatcaat gagtcattca aatcaaaggg agaccaagga tttcggatcg aatttgtatc
    18541 atttggtttt aaatacgggt tgccgatcga ttcggatatt gtgatggatg tgcgtttttt
    18601 gcccaaccct cattatatcc cggaactaag accattgacc ggaaaagatc agccagtcta
    18661 tgattatgtg atgtcctttg atgaaacgca atcattctac cataaatttt tggatcttct
    18721 ggaaactgta ttgcctggat acattaaaga aggaaaaagc agcttgacag ttgcaatcgg
    18781 ctgtacaggc ggtcaacacc gttctgtggc attgactgaa cgtttggcaa atgctttagg
    18841 gaaaaactat aaagtcaatg tgacacatcg ggacaaggat aaaaggaaag agacggtgaa
    18901 ccggtcgtga aaatgaaaac ctaccggata agaaaaccaa agatcgtagt ggtcggcggt
    18961 ggcactggtc tgccagttat cctgaaaagt ttgcgaaatc aaggagcaga tatcacagca
    19021 gtagtgactg ttgcagatga tgggggaagc agcggtgcaa tcagagaatc gatcgctatg
    19081 gctcctcctg gtgacttgag aaatgtctta gtggcgttat cggatatgcc acaattctat
    19141 gaagatattt tccaataccg tttcaaaaag gaagatcagt ttttagccaa tcatacgatc
    19201 ggtaatctga tcattgccgc agtttccgag atgcggggaa gcacttatga ggcgattcaa
    19261 ttattagcaa aaatgatgca tgtagatgga catgtttatc cttcttcaga aactcctttg
    19321 accttgcatg ctgtctttaa agacggaacg actgcagtag gagaatctaa aatcgcgatc
    19381 gaccgtaaaa ccattgaccg tgtgtatgtc tgcaacacaa atgataaaag tgaagccaaa
    19441 gcggctcgaa aagtcgtttc agcaattatg gacgccgaca tggtcgtttt gggacccgga
    19501 agtttattca ccagtatcct tccaaattta gtgataccag aaatcggcga agcaatgctc
    19561 aagacaactg ctgaaaccgt ctatatctgt aatatcatga cccaaaaagg ggaaacggaa
    19621 catttcacag atgccgatca tatacgtgtt ttacacaagc atttgaatca gccttttatc
    19681 gatacagtat tggtcaatac ggaaaaagtg cctgaagatt atatggaccc agagatctat
    19741 gatgagtatc tagtacaagt cgcacacgac ttcaacggat tgagaaatga aggatgtcga
    19801 gtgatttcca ccgacttttt ggaattgaaa gatggtggtg tcttccacga tggagacaaa
    19861 gtagtagaag aattgttccg tttagttttc ggttcaaaat attaagcaat ttattgttgg
    19921 agaaaggagg agaataggat gtcatttgct gcagatgtga agaaagaact aactggtttg
    19981 gaagttcatc gagaacatgc gaaagcggaa ttagctgctt tgctgcgaat gaacggctct
    20041 ttaggtattg tgaaccagca gtttattttg aacgtccaaa cagaaaatgc tgcaattgcc
    20101 agacgtatct attctctttt gaaagatcat tatcaggtac gaagtgagct actcgttcgt
    20161 aaaaaaatga aattaaaaaa gaacaatgtc tatatcgtcc ggttgaagca aggcacaaaa
    20221 gatgtgttga tggatcttga tatcatggat ggtatgatgt tcaatggaca cgtttcggac
    20281 gagattatgg gaaattcgca aaaaatgcgt tcttacttac gtggtgcttt tatggcctca
    20341 ggttcagtca ataatccgga aacgagccgt tatcatttgg aaatattttc tatttatgaa
    20401 cagcataacc aagacatttg cgatatgtta aattattacg gtctacatgc tcgaacgctt
    20461 gaacggcgca atggctatat ttcttattta aaaggagcag aaaaaattgc tgatttctta
    20521 acattgattg gtgcaaccaa ctcaatgctc cgatttgaag atgttcgaat cgtacgggat
    20581 atgagaaatt cggtcaatcg tctagtcaat tgtgagacag caaatctgaa caagacgatt
    20641 gatgcggctt ctaaacaaat cgaaaacatc gaattgatcg aagcaaaggt cggtctacat
    20701 gcccttcctg aaaaacttca ggaaatcgca gaacttcggc ttcaacatcc agaggtcagc
    20761 ttgaaagaat taggggaaat gattccctca ggtgcgatat caaaatcggg aattaatcac
    20821 cgaattcgca aaataaatga atttgcggaa aaactgcgtg aagaagcagt ttaatccaaa
    20881 gaaaaaactg atacatgaag caggagatgc gaatgtatca gtttttttta aaagttatac
    20941 ttgcttttct tccgtcaaac tataattcca tctttactgg agtaacataa tttggaaagg
    21001 cttctattgt ttttaataca atcgtataaa aagcggggag acaactgaat gaagatttta
    21061 gttttgtaca taaatttttg cttaatatag aagtcctgat atagataaga tcaagtattt
    21121 atctataact ggcctgatta attaaaaaac tggaaggaac tgctcgatca tccttgagaa
    21181 ctgctcttct tacttctggt caagattctc gtggttggtg gataaggatg gttgataagt
    21241 tcattggatt agctagtcga aaacaaccag gtgtaattaa tgtagaagtt ttaacaaggg
    21301 gatggagtga tttattcagt gataatcata agttcataaa tagtaattga gatgtatggt
    21361 tggaatacgt tatacctgaa cttgaactac caagatttgg tttagaattt gagaatcgat
    21421 gtttataaag ggagggcaaa aatgcgtttt tttacaactt ttactatcat tatgattgca
    21481 gtacttttta tttttctaga tatagcaaag cgaaatacag catttttatt atatcgggtt
    21541 ttacttcgag caggtctcat aacatttata tcgatcgttg gattttttct attcacagtt
    21601 attgtattta tttggagaac tccagcacca ccattgccag agattacata tggagaattt
    21661 ccttttagac ttgagtatga attgaatgaa gaattacatg tgatagaaga cacgttaata
    21721 gttgaatttg atggatttgg aatgaacgaa ggcataggga gatatcgtag atggacaagt
    21781 cgtttagcca gcggagaaga tttagtgttg ctgttagaag ttagtgataa taagcaaata
    21841 ttttattttc caggacctgc taattattac atgggagata ggttgaatgg ttataatcat
    21901 acttttccta gtgcttcttt cattgaaagg gaaagaggca ttactagaag ggatatttta
    21961 catgacaaag aattattaga acaatttggt ccgcttgatc aaaatacaat aaatgaagaa
    22021 gaattgttaa atcaatataa tataaggcta gtgaattggg aaatatctga accgattgtt
    22081 aacaattttg gtgattagaa aacaattacg ataaatagaa acaagccaaa tcatcagcaa
    22141 gtaaaagctg atgatttggc ctttcttata cgagt
//
LOCUS       DACDEG010000039        21903 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.041, whole genome shotgun sequence.
ACCESSION   DACDEG010000039 DACDEG010000000
VERSION     DACDEG010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.041"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..643)
                     /locus_tag="IV142_001495"
     CDS             complement(<1..643)
                     /locus_tag="IV142_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8485160.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFII"
     gene            complement(793..1506)
                     /locus_tag="IV142_001496"
     CDS             complement(793..1506)
                     /locus_tag="IV142_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8485161.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            1681..3105
                     /locus_tag="IV142_001497"
     CDS             1681..3105
                     /locus_tag="IV142_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8485162.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            3404..4111
                     /locus_tag="IV142_001498"
     CDS             3404..4111
                     /locus_tag="IV142_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP8485163.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            4098..4421
                     /locus_tag="IV142_001499"
     CDS             4098..4421
                     /locus_tag="IV142_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP8485164.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(4510..5547)
                     /locus_tag="IV142_001500"
     CDS             complement(4510..5547)
                     /locus_tag="IV142_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8485165.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(5868..6851)
                     /locus_tag="IV142_001501"
     CDS             complement(5868..6851)
                     /locus_tag="IV142_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP8485166.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(7266..8036)
                     /locus_tag="IV142_001502"
     CDS             complement(7266..8036)
                     /locus_tag="IV142_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8485167.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(8029..8964)
                     /locus_tag="IV142_001503"
     CDS             complement(8029..8964)
                     /locus_tag="IV142_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8485168.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(9280..10929)
                     /locus_tag="IV142_001504"
     CDS             complement(9280..10929)
                     /locus_tag="IV142_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP8485169.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(11046..13649)
                     /locus_tag="IV142_001505"
     CDS             complement(11046..13649)
                     /locus_tag="IV142_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8485170.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(13661..14710)
                     /locus_tag="IV142_001506"
     CDS             complement(13661..14710)
                     /locus_tag="IV142_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAP8485171.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(14736..15050)
                     /locus_tag="IV142_001507"
     CDS             complement(14736..15050)
                     /locus_tag="IV142_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP8485172.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(15066..15512)
                     /locus_tag="IV142_001508"
     CDS             complement(15066..15512)
                     /locus_tag="IV142_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8485173.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(15513..17489)
                     /locus_tag="IV142_001509"
     CDS             complement(15513..17489)
                     /locus_tag="IV142_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAP8485174.1"
                     /translation="MKIEIIIVHLSCIQEVKIMGIEFSERQMKILYFLIENEHTTYKE
                     LSEQLYVSTKTISKDILILKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKIN
                     IADSPEDRLTYVFAKLLNTNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLV
                     SDRNGVRIKADEKSKRRLMSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDI
                     LKKVAELLNRYMNKTQLKFTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNT
                     LILIKMIEKAFSIIVPIDEQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVR
                     IKLNEFYPDEELINNLVLHLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEE
                     IEERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFA
                     DMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQL
                     NEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKL
                     SSTAMNDFAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKI
                     YETFNEIASNHELLANLKTSSSFEKLVYILKEGL"
     gene            complement(17647..18543)
                     /locus_tag="IV142_001510"
     CDS             complement(17647..18543)
                     /locus_tag="IV142_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8485175.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(18536..19216)
                     /locus_tag="IV142_001511"
     CDS             complement(18536..19216)
                     /locus_tag="IV142_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8485176.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(19217..19999)
                     /locus_tag="IV142_001512"
     CDS             complement(19217..19999)
                     /locus_tag="IV142_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8485177.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(19992..20909)
                     /locus_tag="IV142_001513"
     CDS             complement(19992..20909)
                     /locus_tag="IV142_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8485178.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(21073..21396)
                     /locus_tag="IV142_001514"
     CDS             complement(21073..21396)
                     /locus_tag="IV142_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8485179.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
BASE COUNT         6530 a         4504 c         3623 g         7246 t
ORIGIN      
        1 tgattataaa tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat
       61 taaaagactt taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat
      121 tattagaaag tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca
      181 ttcctgaagc tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg
      241 cattttcagg agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa
      301 ctaataccgc tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg
      361 ctgctacaac taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt
      421 gttgtaacgt ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta
      481 agatcactga attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag
      541 caaacgcatc tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac
      601 caaatttttc aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg
      661 cttattgtaa tcgattactt tttccaccca aataatacat cacttatcaa aaaaaagtaa
      721 atacttttaa ttaaatatat atatcttttt tagtataaaa aaataagctc tgaaaaaata
      781 attttcccga gcttattttt caacaaaaac agcttcaaaa acaaagtctt gatataaatg
      841 tctcgatacc gaatactcga aaggtttacc agacgataga gcaacaactt tgtctatctc
      901 aatcataaat tcacctggat ctatttttaa tatttttgcg tcaaattcat tcgcagtatc
      961 tcctttgata cgtatgtgcg aagtctttat ttccagaccc agttcatttg tgatataagc
     1021 atatacagat ttctttaaat gttttggttc caagcctggt atgactgcta atggcatgaa
     1081 tgtttgctca atcgagtatg gttgacaatc aataaaacgg cttcgtacaa tattatatac
     1141 atagtctttt acactgatac ttaatttatc agctactttt ttcgatggaa gttcaattga
     1201 gaaagtatgg atttctgaat ctacgtgttc ttcaccaacc acgctaaaca aaccttcgtt
     1261 tggaccaaga actcgtgagt tatcaattgg tgttctgacg acttcagtcc catacccact
     1321 tctacttcgc aaaactcctt cggaaacaag taagtctacg cctttcttta ctgttaactt
     1381 gcttgtatga taaataactg ccaattcatc tcctgtagga agtttctcac caattttata
     1441 ctctccattt acaatttttt tctttatatc taagtaaatt tccatatact tcgcaatttt
     1501 tttcaatggt gaccctccaa tcaaaaacat atcttcatct atactttaac ataaataaac
     1561 cgataaaatc cacatcaaaa ttatacacac aataaaaaag atatatatat ttaaacaaaa
     1621 ctatttactt taagttgtaa gcggtgttat cattaagtgg aaacaggagg taaaaaaatt
     1681 atgacaacta tgaaaaagct aacttttcca gatgattttt ggtggggttc cgcttggtca
     1741 gctgagcaag cagaaggaaa aggagaaaca ggaaaagcag agactatctg ggatcgctgg
     1801 tttaaagaac aaccttaccg gttttatcaa cgaatcgatt cctctattac aaccgatcac
     1861 atccatcaat ataaagaaga tatagaacta atgaaaaaaa ctgggcataa ctcctttcgc
     1921 gtttctattt cttgggcacg aatgtttcca aatggcggta ccggaaaagt aaatcccaaa
     1981 gccatttctt tctatcgaaa attattttcc gaaatgaata ataacggaat caaagtattt
     2041 gccaacttat atcattttga catgcctgct actcttcaag ataaaggagg atgggaatca
     2101 cgtgaagtag ttgatgctta tgttcattac gctgaaactt gttttaaaga atttggtgac
     2161 ttagtttgtc attggtttac cttcaatgaa ccacttgggc ctattttagg tacttatctt
     2221 gaagattttc attatccaaa cctcattgac tttaaacgcg gagcgcaagc agcattcttc
     2281 actatacttg cacacgcaaa agcaatacaa gtatttaaag aactagctct tcctagtaaa
     2341 ataggcgtta ttctaaattt gagtcctact tttcctcgta gtcaaaatcc tgctgatgtg
     2401 aaagcagcag aaatcgctga tttattttat acgcggagct ttttagatcc tatggttaaa
     2461 gggaattttt ctaaaaagct tatcctgtta ttacaagaat ataatcagct tcctgaagat
     2521 tatacgaaag aagatcttgt atgtatctac gaaaataccg ctcaaattct tggactaaac
     2581 tattacgagc cacgtcgcgt caaatctcgt tcttctatgg tggatacatc tagtccattt
     2641 ttaccagagt ggtttttcga accacatatc atgcctggac gacgaatgaa cgaataccgg
     2701 ggatgggaaa tttacgaaaa aggaatttat gatttgtgca tggatatcaa gaaccattat
     2761 ggaaacatcg aagcatttat ctcggagaac ggaatgggcg ttgcggatga agaacgtttc
     2821 atggatgata cagggcaaat aattgatgat tatcggattg agtatatcaa agatcatctt
     2881 gcttacttat ggaaggcaat aaatgaagga tgtaacatta aggggtatca tctttggaca
     2941 tttattgatt gctggtcatg gatcaacgcc tataaaaacc gatatgggct ggtttcactc
     3001 gaccttacta ctcaaaaaag aacaattaaa aaaagtggcg aattttacaa gcaattaagt
     3061 gatacaaatg gatttagata cgatgtagac ttgttagttg aataatattc taaaattcca
     3121 cttgaagtga acacattaga aaacaagaat aatgagaaat accaataaca aatacttttt
     3181 cttcatactt catttatact tgtcagtata agagactgtg acaaatgtag tgatcctcag
     3241 aagttaggtc tggaaatcta acttgagaag atttttttaa actacgtcca gtctttttta
     3301 tttgtttaaa attgaagttg acaagtcttc attaaaaatt tagaatattc acaagtttga
     3361 cgacaattat gaaaatttat gtcgtgctaa aggagttttg ttattggatg aaaagttaga
     3421 tataaaaact gcgatcaaag acacgttacc taccgttttt ggttatatcg gtattggact
     3481 tgcatttggt atcgttggga aagctgccgg gtttcatccg ctggtcgtca cgttgatgtc
     3541 cctgctgatc tatgctggtt ctgcccaatt tatcacggtc agcatgcttg ctagccatag
     3601 tccactgctc tccatcgttc tctcgacctt tctagtcaat tcccgaatga ttctgatgag
     3661 tatgacgatt gctccttatt tcaagaaaaa tcggctgctt caaaatctgc tgattggcac
     3721 attgctgaca gatgaaagtt tcgcactggg aatgaataaa ttgaactata ctggacaaaa
     3781 gttaaacttt cgttggatga atacagctaa ttggatttct tatttgacgt gggtgggttc
     3841 ttctcttgtt ggcgcacttt tagggaattt tattactgat ccgaaaaagt ttggtttgga
     3901 tttcgccatc gtcgctatgt ttatcgggtt actttatctt caagtcattt ctgatagaaa
     3961 cacctctaaa cgattacagt tgatcctgat tggtctcact ttgattctgg tatatgtcgg
     4021 actgattttt atcccaagta acttagtgat tttagttgta acactgattg ggtgcggatt
     4081 gggggtgtgg atcaaacatg ccttcttttg agtttacatt gctgactatt ttaggttgta
     4141 ctttagcaac ttggctttct aggatcctac catttgtctt actgaaaaaa ttcgatttgc
     4201 cgcaaccgtt gcttgagtat ttaagttttg tcccgatcgt cattatgtct gctttatggt
     4261 tcagcagtct atttacccaa aatatcggac atctgccgca gatcaatctc gaaaatgctt
     4321 tggcttcagt tcctacactt ttagctgcta ttctttcaaa aagcttattg gtgatcgtct
     4381 tggtaggaat tctttccctt tcgttgattc gcttattata aataaaaaaa caagagctta
     4441 tggcaaatta ctgtcatgcg ctcttgtttt tatcaaagtc aaataagaaa aaaatcggcg
     4501 tattcttgct tatccgttga acggaccact aacaacgact tttcctaata tatgtcctgt
     4561 ttctacgtct ttggttgctt gtttcaagtt cttcgcattc atcccatctg aatagacctt
     4621 gctcaatgta ctgcggataa tcccttcatt tgctaaagca gcaatgtgcg ctaaagcttc
     4681 tccttgcgtt tcgatttcat agttatagtc tgttttagcg aacatatact cccagtcgaa
     4741 gctgatagat tggtttttcc aaacacctat atctaacggc tctttgatcc ctacgatcgt
     4801 tcccacatgt ccaaatggtc ggacaaggtg tttgatttga tcaaagtagc gggtaatatc
     4861 gaataataca gcgacatact ctacttgatc gattcctaat tcacgaaaac ttttacttaa
     4921 gtcgtgatga taatcgattg gatagtctac acctgttttt ttcagccatt caaaattctt
     4981 cggacttgca gtcgcgtaga cttctaggcc agcccaatgc gctagttgat tcaaaatcga
     5041 accgacgccc ccagctccgt tgatcactaa gatttttttc ccgcggttgg cattttcttc
     5101 tggagtcaga ccaaattttt caaataacaa ttcataagcg gtcaatgagg tcaacggcaa
     5161 ggctgcggct tcttcatctg aaagattttt tggtgcaaga gcgacaatac gttcgtcaac
     5221 cagttggtat tcttgattgc ttcctgctct tgtcgttgta cctgcgtaaa aaacgcgatc
     5281 gccgacagaa aatttttgca cttcatcacc tacagcaaca actttcccga ctccatcaaa
     5341 ccctaagata cgtaaagcat ttcttatccc gttatcttgg cgaagtttcg tatcgacagg
     5401 attaacagat actgcgttga cttttaccag aagatcccgt gaactgggta caggtatctg
     5461 ttctttttca tcaataaagc tttttggatc atcgatcgac aacccttcat aaaaaccgac
     5521 taccttcatt aattcctttt ctgacatacg aattcctcct tagcttcatc aagttggagc
     5581 atccatacag ttttcatttc aatatatata gtatgctatt tgttccactt ttattcatca
     5641 taattattct cttacttaaa gtaaactatt tgcacttttt tggttaaaaa agaggttgcg
     5701 acaagacttt tgtcacaacc tcttttaaac ggtgttcaat caactgaccc tcggtattcg
     5761 ctcaaaaaac gacaaaatat gaggagctat ttttgtttgg ctgttcttat tacttgatgc
     5821 aggacagctt tttcacgacc tctaattcac ggtgttcaag caagaaatca ttgttcactg
     5881 aaaagcgttg gatgttcttt ttcaaaggtg tctaagatct ctcgtacttc atcagtagat
     5941 ttcgtattca tcaactgaac tcgcaagtca tttgctcctg gaaatccttt gatatagatt
     6001 ttaaagaaac gatgtagtcc tgtgatggaa cgcgggatga tttctgaata atgatcttgc
     6061 agatctagtt gcagacgaag tagtccgatc agttcttttt ctgtatgttc cctcggttct
     6121 ttttcaaatg cataaggatt cttgaaaatc ccccggccaa tcatgatgcc atctacgcca
     6181 tatttttcgg ccaattcttc tcctgtctgg cgatctggga tatctccgtt gatcgtgatc
     6241 agtgtttgcg gagcgatttg gtcacgtaaa gcaatgattt tagggatgac ttcccaatgt
     6301 gcatctactt tactcatctc tttacgcgta cgcaaatgga cagaaagatt ggctatatct
     6361 tgatgaagta ggtgactgat ccattcttcc atctcagaca tttccgtata gccgatacgc
     6421 gtcttgacgc tgacaggcaa accgccagct ttagccgctt caatcaattg tgccgctact
     6481 tctgggcgaa ggatcaagcc actgcctttt ccacgttctg ctacattagg gactgggcag
     6541 cccatgttga tatcgattcc ttggaagccc atttctgcca tagcaatgct catttctcta
     6601 aaaaattctg gcttgtctcc ccaaatatgg gcaaccatcg gctgttcatc ttctgtaaag
     6661 gtcaatcttc ctcgtacact gtcttttcca tctggatgac agaaactgtc tgagtttgta
     6721 aactcagtaa agaagacatc cggtgctgct gcatgcttta cgacatggcg aaatacaaca
     6781 tctgttacat cttccattgg tgctaaaaca aaaaaaggct ttggcagctc tttccaaaaa
     6841 ttagtagtca tttatttgtt actctccatt ctatcaagca gtgaagtcgc aatctttctc
     6901 aaaaacattg tcattatact aggaaacaaa aaaagaagca aagtgaacag cttaaaagat
     6961 gtttctgatt gagcaaaaaa agactgtgac aaaaatctta tcacaattcc ttttccggaa
     7021 taaatggtgt tcaaaagctt gcatctgcgg taataagtcc aaccaaacga aaaataggag
     7081 gagctatttt tgtttggctg ttcttattac ttgatgcaga acagcttttt cacaacctct
     7141 ccactaaacg gtgttcaatc agctgaccct cggtattagc tcaacaaacg acaaaatatg
     7201 aggaactatt ttcccgtttg tttctctaat actcaggtca aaacgctgat ttcacaacct
     7261 cttttctaac ttctcaatcc tcttccttta ttgatcaaat accaagtaac ggcgtacaaa
     7321 acaacgatga atagcacaag tacgatcatc gatgttgcta ctggcacatc gattgttcca
     7381 aggaatccgt aacggaaacc ggagatcata taaacgatcg gattgacttt cgaaacagct
     7441 tgccaaaatg gcggcaacat agagatggca tagaaaacgc cacctagata agttaacggc
     7501 tgcaagacaa atgtaggcac gatcgataca tcgtcataag actgagcaaa aatcccgttg
     7561 agcagtcctg ctaacgaaaa caagattgcc gtcatcaata atgtaatgat gaccatgaac
     7621 catgaataca catgaagtgg tacaaagaac aaggaaatga tcgttacgag agagccgacg
     7681 agaatactgc gacccactcc gccaatcaca aagccccaaa tgatcacatg cgtaggaaca
     7741 ggcgctacca agatttcttc gatgtttttc tggaattttt gagagaagaa ggacgaagaa
     7801 acgttggcat acgaactagt gatagcagac atcataatca aaccaggcac gatgaattcc
     7861 atatatgaaa aaccacccat gtcaccgatc cgaccaccga tcatttttcc gaaaataaca
     7921 aagtacaagg atgtcgtgat cacaggcgga accagtgttt ggacccagat acgcaaatag
     7981 cggttcgttt ccttgatagc taaacttttt aatgcagtaa aataaagatt aaacattttt
     8041 ctcctccgta tgattatttt cttctgtgat tttcaaaaac agttcttcta aacgattcga
     8101 cttattcctc atagaaagaa ctttgattcc ttgtcttgtc agctgatcga agattccgtt
     8161 caccccttga tttcgttcaa cttcgacagc gattgtctgt tcatcttcaa atgtacaagc
     8221 gtatccttca atcacaggtt tggtctcata tggcgctaaa tcaaagataa atgtttcaaa
     8281 ttgcagtttg gatagaagct ctttcatact agtattctcg atcagttcac ctgattggat
     8341 gatcccgatg ttgcgacaaa gcatttcagc ttcttctaaa taatgcgtcg tcaaaatgat
     8401 cgttgtacca ttttcgttca attctcggag aaactcccac atctcgcgtc tcaattcaat
     8461 atcgactcct gctgttggtt catccaagat cagcaaacgt ggttcatgca tcaatgccct
     8521 agcaatcatc agtcttcgct tcattccccc ggaaagcata cgtgcacgga catttctctt
     8581 ttcccaaaga ttggactgct tcaaatattt ttcgctgcgt ttcatcgctt ctttgcgaga
     8641 gacaccgtag tagcctgctt gattcacgac gatttgctgg accgtttcaa agggattgaa
     8701 attgaactct tgcgggacta atccaatctg ttgctttgct cggactagat ccgtatccag
     8761 atcataaccg aagacattca cttttccaga tgttttattg actaaagaag taatgattcc
     8821 gatcgttgta gacttcccag ctccatttgg tcctaatagc gcatagaaat ctccttcttt
     8881 cacggaaaga tcgattcctc gcaatgcttc gaccccactt ccataaacct ttttcaaatt
     8941 atttatttca agcgcatttg ccatttattt cctatctcct tatagacatg attctttatt
     9001 gtatcatgga atacagcaga tgaaaaaaca acttggttcc agcgaaaaat caggttgctg
     9061 ggctcaggaa aatctcctaa tgaaagacat caatcggacg aagtcagaca accttttcag
     9121 aaaaaacaaa tcgaagataa aggattacta ttcatatgtg caacaaaagg agcgtgagac
     9181 aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg aagcaaaagc
     9241 caagattttt gatccaataa atactttcga tcacttcgcc tatcccaata catgccactg
     9301 ccgcttttca tcaaataacg cggcttccgt ttttgcggtt tctactaaag tctctgggta
     9361 atccaaaact ttcagcaagg caatcgcatt tcgggtcctt gaaggtcctt cttttaattt
     9421 gaaatcaaaa ctgattcctt ttccttctgt tacttgttct tcaaagtgga cattctcaca
     9481 ataattcttt aaaatctctg tcaattcgat atcatgtgtg gcgacaaatg ctaaagatgg
     9541 atactccgca agccaccgaa cgactgaagc ggaagaagca atccgttcga tcgtatttgt
     9601 cccttttaaa atctcatcta cgaagcagta gcagcgttct ttggaagcca cttggtccaa
     9661 caaacgtttg atcgatttga tttctgaaac aaaataactg tcgccttcaa ataaatcatc
     9721 ttcgattgcc atggaagtca acacatgtcc aggttgcagg gtaaactgtt ctgcaatcgc
     9781 tgtttggatc gtctgtgcta gaatacagct gatggctata cttttgacat acgtagattt
     9841 tcctgatgca ttggaacccg tgactaatgt attcttctgc cagttgaccg gattcactac
     9901 cgcttctttt aacaatggat gatagatatc tttcgctgtc acaccgccct cagtaaactc
     9961 tggtcggcaa gtgatgggca tataagtacg gtaattcaat attgcagcgg ctacttctaa
    10021 ttctcccagc aattcccaca gcttgatggc ttcagttgaa tgcttttcta atttagtgag
    10081 aacgaaatga taggagataa atggcagcat gaacatgatg ttgacatatt caaaaatcaa
    10141 ctctgcttca ctcccactct tcatacgaaa tgaaaaacct aatttaggga tgtctcttaa
    10201 tggtttagtt tgctgtttca gctcttcttg aagtggtgtc ggcagttttg ctagctgatt
    10261 ggcggctgaa atcgttgaaa ccaaataact catgctattc agctctgttt ctagtgtttg
    10321 tttcttcact agataataga ccgtattgaa tactaaccca ccgattgcca aatagattcc
    10381 tagtacttgt cctaaaagaa gcaaacccaa acccaccagc ggaagcaagc caagaaaaat
    10441 aaataaacct acgcgtccaa tctttttact ttttccatcc gctagatatt gttttgcaaa
    10501 attatgatct ttttttccta aacgagcgaa ttgatattgg attttttctc tcagttgagg
    10561 attttcctga taaaaagcaa tcagtttttc taactgctga tcttcaccaa aatcaaagct
    10621 gcgtaaacgt tgatacaacg cttctgaacc tacactagaa taggtactgt tgattccttc
    10681 gaatacttcg aacatatcca ggtcatacca agtaagatca tcgatttcgc tatcccatga
    10741 acggaacttc ttctccgtga gccatgcttc cttcaagctt tcttctttat caaatctcgg
    10801 ttgataagga attttttccc atttgctacg aactgtttcc tttaatttca aacgtgaaaa
    10861 aatgtctaaa gcaactaata aaataacagc tgcaataata attaaaacga taaattctct
    10921 ttgacccata tccctctccg ttcctttatt tgttagcttt agtatagcac tgttgcgcta
    10981 tttgaaaaga gggtaaaaaa acacctttca aaataaaagg tgtttttttc ttgtatatta
    11041 catatttatc tctcatttat caaaattgac aaataatcat agggaggaat tgttgttaat
    11101 tcttgtttat tctccagtgc attgacaact gtcccaaggg aaaggatagc ctcaggtact
    11161 attttttctt gttctgttgg attagctaag cgaagaacaa atccttgttc atgaaaataa
    11221 gaaggataaa tcgatgatac aattaagtct tcaggtaatt caatcaatga aaatttacga
    11281 ggaacatcaa gttcattttg taatggccaa atcttgttat ctaacctgtg aataaataca
    11341 ttcagtttct gcttttgata agaaatagat tgttctaacc gtttttctgc ctgttttgcc
    11401 actttgaact cattaaatct tccttcttct acattaaatc caaaagtgac tttatactca
    11461 cctatctctt gagctagcgg tgtttccatc atgacatgcc cttcatttgt cgtatctcct
    11521 gaagctcgcc caggtcgcca agcaagatta ggttttccaa gttcccctgt tgttgaaaat
    11581 aaggttatga ttaattgatt ttctatacgt tcatactctt ttaaaccatc tgcataaaac
    11641 gtaaaataat gatcattttc tgccacagag acactcttat cgaatatttc taaattaaca
    11701 ggtttctcta ccatagtttc tgaccaattc ggaggaatga catcaggggt attttcaata
    11761 aatccgtttt gaatttgtgc aattgatttt tcagatttat ctaaaacatt aattgctaag
    11821 cgcatacgat gcgagtaaac agtattatga attttgagtg tgccttcaac gatttgactt
    11881 tctcccagac tgaacgatat ttcataagat atttcttcca tttcgggttt ttcagataaa
    11941 cgatctttta aatctttagg aagttgagct tttccgtaaa caactaatgt ttcttgtaaa
    12001 gaatctttat atactttagc tgtttctaat tttagagata gttctgtatc tccttctaat
    12061 ggtgaataat catacgtatc gccatcattt gctgaatcga tcaaatgaac gaaatcaaga
    12121 tattgtctat ctccaactat caaattcaat tgcccatctt taaaagaaag tttatattta
    12181 ttgttactta tctcagtttt gtctatctcg attgctgttt ctaatttttc attatttttc
    12241 tcaaattcaa caacagtgta tcctagtgct ggcacattta catttatttt tatgtcaagt
    12301 tcataataag ctggttcatc aaaatattcg aaacctgtag caatcatccg ataagcattt
    12361 ttccgttctg gaaaataaac ttctttttca atgatattat agggactacc tttgaagtgt
    12421 attttctttg aatcagctac tacatgcacg attttcgatc cactataatt ctcagtcgtt
    12481 gtgttgaaaa caaggacttc attcgatttt agctttaatg catcagccat ccgttttaca
    12541 ataaggtttt cgattccgtc agctatctca ttagcttctt tgattcgatg gaaaatatct
    12601 tccgcaacat tatctgaaac acagccacca atactatcat gagcctggtt ttgcaaaatc
    12661 tttttccata accgacgaag taggccttta ctgatatgga taccatttct ttctgcaatc
    12721 agcatgagcg gctcaactcg ttttagaatt ttttgttcca atttaaaatt ttctattttc
    12781 atttttgttc ttactgagcc aatagaacga tgaacacgag agtaaaccgg ctgacgaagt
    12841 tcacctttat attcaggtaa ttcttttttg tttctgataa cttctacata agaaggataa
    12901 tctgaaataa tattgtggaa tttactgtat ttattcaact tctctacgac ttgatcaaaa
    12961 tttaaaatca ttgacttttg atctacaccc gaaggtacta aaatatcttt gttttttcct
    13021 tgcatatgaa gcatttcaac tgcttcatcc aacttttctt ttgcaaattc aggaatatct
    13081 ttaattgcgt ctaaggtcat cattccagtc atataaccaa atggaaaatt catggcataa
    13141 acaaatttat ctcccaatcc tttccagata aaataaggag atggaaccat cgtttcaaag
    13201 tttaatcctc gccaacacat gaaattatcg atttctactt gatgcaaaag tgtaggcaaa
    13261 ttcgcattga acccaaacgt gtcaggcaaa taccctacca tcattggatc tccatattta
    13321 aggatcgtat cactgattcc aatcattaag tttcttaaaa ttgattcagc atccactaat
    13381 aaggcatctg tttgtgcata ccacggtccc acgaatagtt gtcctgtttt catcagtcgt
    13441 tggataacct taagcttttg cggattgatc tccaaatact catcgacgat tgaagtttgt
    13501 ccatctaaac aaaagtttac tttagggttc ctttccaatt catctagaac ttcagtaaag
    13561 acctgatcag acaatacgat ggcatcttca tttgtaaaat accattctct gtcccagtgc
    13621 gtgtgttgaa ttatatgtgc atttaccata tcagattctc ctactttcct tcttctactg
    13681 aatttacttg ttgttctttt ttatacttga tggattttag tgcattgacc attaacgctg
    13741 taaccactcc cccaataaca attgatacta aatataaagg tatattcgta acaaaaggaa
    13801 ttgccataaa ggttgaaagc attgtttctt gagtaacacc aaaaaatcca tttagaccac
    13861 atgctactgc agtgcccacc acgatgcttg gtattactcg taaaggatct ttcacggcaa
    13921 acgggatcgt cccttcagta atccccacaa ggcccataat catagctgtc tttgcattat
    13981 ctcgttccga ttcatcataa agttttttgt ttaaaattgt tgctaaaaac attccaaatg
    14041 gcggaatagc aattcctaat aacatggctg tactaggtcc aaagcttccg cttgaaaaag
    14101 aagcaacccc aaaagcatat gcgattttat tgataggtcc tcccatatca aacgccatca
    14161 ttcctcctaa aatagccgac atcaaagcag catttgctcc tgacatgcct tctagccaat
    14221 tagtcagtcc tgttgtcatt gcttgaatag gcgtagctag tacataccat aatatgattc
    14281 caacaactaa tgtagtaaca aaaggaatca gcataattga ttttagacta ataacttgca
    14341 tcggtacttt tacttttttc aatgcattag ctaaatagcc acaagctaat ccagcaacca
    14401 ttcctccaat aaatcccata ccaagatttt gacacatcat cccagtgata aaagctggtg
    14461 ctagaccggg acggtcagca attgaatatg cgatataggc tgaaatcatc ggtacgataa
    14521 actgtaatcc cgtctgacca atcattcgca ggactcccca gaatgtatcc gtctggtccc
    14581 aaacacctgt gccaccaaaa atgactgcaa tagaagttag taaccctgct gctacgacaa
    14641 gcgggatcat ataggaaacc ccactttgga aatgatttag aatttctctt ccttgtttct
    14701 tgaattccat tagtaactcc cccacgatat attttttatt tgttaattaa tgcaattgct
    14761 tcatcaatcg tctcttcacc atcttttact acctctgagg tccctactgt taaaatcggt
    14821 ttaccgtcaa agcgacctcg ttctgaaaca tttgtatcta ctgcaaatat gacaacatct
    14881 gcctcggaaa catccgaaat tgaaagtttg ttttctatcc ccatagcccc ctgtgtttct
    14941 actttaatat ctaacccttt cttcttcgca aacttttcta aattagcttt tgccatataa
    15001 gtatgggcaa tccctgcgat acatgctgtt actgctacta tttttgtcat atgaaattcc
    15061 tccttttaaa atgatagagc ttttaccaat tcttttttat ttctcgaatc ttttattctt
    15121 ttaacaaact cgtcatccat caatcttcca gataattctg ctaaaatacg taaatgatta
    15181 gtacctttat cagtgtcttt tattgctaac aaaaagacga gatcaactgg ttgatcatct
    15241 aatgcgcccc attcaacaga ccgctttaac ttggctacag ccactgttga ttcaattact
    15301 gctaggcttt tgccatgagg aatggcgata tttttcccaa ttcctgtggt catatggttt
    15361 tctctgtcaa atacgtcttt ttgaaattgt ttgtggtcag acacaaaccc attagtctct
    15421 aacaatttcg ataatttttc gactacactt tttttatcag atgcatttaa atctatcact
    15481 atattattta tattgatttt ttcactgatc atttataagc cttctttcaa tatataaact
    15541 aatttttcaa acgacgaact cgtttttaaa tttgctaata actcatgatt cgaagcgatt
    15601 tcattgaatg tttcataaat tttattcaat tcaggtttta ctttttcatt aagtgcaaaa
    15661 aagaaaacag aatgaacaag tgtcccatcc caatcaattc cttctggatt aacgtagaca
    15721 cagattactg gcttttttat aaaatctact tcgatatgcg gcatagcaaa atcattcatc
    15781 gctgttgaag atagtttttc tcttttgatt gctccctcta gtatcccttc tttcgcgtaa
    15841 ttttctttta ccaatttttt ggtaattata tttaaaacgt tcacttgatt atcctttatt
    15901 tggtgggtaa aaaagcaaaa tcgtttatct aataaattta gaaaagcatc ttctgtttgt
    15961 tcatttaact gtagatactg ttttactttt ctgacatctg cccctaacat caaaggacta
    16021 acaacaataa caggaaaatt tgtattttca atcggaatcg tgcttataac aagttcatca
    16081 gtgacttttc tatcttgtag ttcattgatg cttagtacat cttctatctc aaccatatct
    16141 gcaaaaccgt tatttattct ttgctctaat aattttgatg ttccatatcc gctactacaa
    16201 actaatatca ctttcgtttt gtcaggtttg taacgatcca aaagtgattg aatatggagt
    16261 gttacgtaag cgatttcatc ttcattaaaa tgtatacaga atctctcttc gatttcctct
    16321 aataaatcta cacttatcat aaatgacctt ttaaaagaat atttgatttt gtccgtataa
    16381 gggttataga tatttactcc caatttgagt cttttaactg ctgcattgag atgtaatact
    16441 agattattga tcaattcttc atcaggataa aattcattta atttaattct tactaactct
    16501 tgtagctcaa cacccatatc taaataggaa agcttcgttt gatctaagtc gtgattcata
    16561 gcattttttt caatcgaagt aatgtgaatg tcaacataag cttgttcatc tattggcaca
    16621 ataattgaaa atgctttttc aatcattttg attaaaataa gtgtattctt tgagatttct
    16681 tcttcagaat tattactacg tgataagaaa ttgcttgttt caatcctttc tatcgcaata
    16741 gcaaggtgga tagctaaagt ttgaaattca taatctgtaa atttcaattg tgtcttattc
    16801 atgtatcgat taagcaattc ggctactttt ttcaatatgt ccttatttaa gaaattattc
    16861 gcttcaccaa tgaaattgat attcaagtct aagagatctg ttttactgtt agatgctgta
    16921 aagccacccc aataatagtt cacaacttct gacatcaacc ttcttttact tttttcatca
    16981 gctttgatgc gcacgccatt tcgatctgaa actaaggaca tcccatgctt gtttagtctt
    17041 tttctaacat ctttcattgt gctttctacc gtcgacctgc tcacgtataa ttcgtcagca
    17101 agattttgaa tctttagaga atcattagta ttcaataatt tagcaaacac ataagttaga
    17161 cggtcctccg gagagtctgc gatattgatt ttatgactgg ataataaatt tcgaagtgtt
    17221 tctttttttc caataagcga aacgcctttt ttaggttgaa ccaacatttg tacatctggc
    17281 attttttgga ttatttcttt caaaattaaa atatctttgc taattgtttt cgtagataca
    17341 tataattgtt ctgataattc tttgtaagtt gtgtgctcat tttcaatcaa aaagtatagt
    17401 attttcattt gccgttcgct aaattcgatg cccattatct tcacctcctg aatacagctt
    17461 aagtgtacaa ttattatctc tattttcata cagaagaaag ttcttgatta atgtagaagt
    17521 ttctaatatt cttgattatt tcaaatcaat aaaaaagcct gcaatattta tatcacaggc
    17581 ttttttctct attaaatata tataagataa tacatttttc tactagttct cactaatctt
    17641 gtataattaa accctccgca acttgacgga gatggagaag atgcccccgt ctaatctagc
    17701 ggtcatctct cctcccattc cttccaccaa tttttttgac aggtaaagcc cgattccgct
    17761 agattccgta tttgtgcggc tggtattttc ggtataaaaa cgctggaaga ctttttcaac
    17821 agaggtttgc gttcctggtt tgataggatt ggccactgtt aatcgaatga atggatcctc
    17881 ttttgaaaat gtcacctttg cttgtccatc tgcataacgc aataaattct gtatgatatt
    17941 ttgcagcact cgcagcaaca tgagcggatc tgctaataca gttgtattta tcgagtgttc
    18001 agcgaacgct acctcgatat ttttctcaga aaattgttca taaaaagaga aaaatacttc
    18061 ttccacctct ttagaaaaag aaagcgtatg gagatccatt tctttttgct tcgtctctat
    18121 tcgagcaaga tcataaaact gatccgtcat actgagtaat cttgcaacag attgtttgat
    18181 gatcgttaaa tacttctccc gtttctccgt atcctctacc tgttccagta attgtacata
    18241 accgttaata gcagtcaaag gagttctgag atcatgagaa aggccgacga tcgatagtct
    18301 gatgttttct tccatttctt gaaatgcttg tcctttttgt tcattttcat ccaaccatgt
    18361 attcagtgca tctactaggt ccattagatt cttttctcga aattcgactg aaagacgacc
    18421 accatattct tgtcgttcag aaagagtggc gattttctta gttaatctct tgatttccct
    18481 ttgcaggaag aaaataaaac ttcccataaa gataacaacg agaagcaaca gccatttaat
    18541 caaatctaaa ccccactccc cacaccgtat gaatatacac agcatgtgcc tttgctagct
    18601 tccctcgaag attggaaata tgcatattca ctgttttctc actatcaaaa tatggttctt
    18661 gccaaactgt ttcataaaga tttgctcgcg aaaatacttt tttcggatgt tctaaaaaaa
    18721 gcttcagcag atcgaattct cttcctgtca aatggacagc cacaccattc acatttacat
    18781 ccctcgactc tttgtccagt aagatttcct tgtaatagat gcgttgcgac agatctttta
    18841 cttggacaga agtctgatga cgaagttgga tgccgattcg taacaaaagt tcttcgatat
    18901 caaatggttt cgtcacatag tcatccgctc ctgctgccaa caaggaaact ttatctcttt
    18961 gatcgctttt tgctgaaaga acgatgatcg gaacattcga tgtctgtcgg atttctgcta
    19021 acaatgcttc acctgaaagc cctggaagca tgagatctag aaggatcaaa tcgacaggat
    19081 atgtgcttaa caataatttt ccttccgtac ctgaataagc atcaagaagt ttgtatctct
    19141 gttcaagaac ctcctccagt aaggaatgaa cagcttcatc gtcttcgata atcaaaatag
    19201 tttccagcag gaccacctac ttaatgtcag atttttggaa aataaacatt cctagagctg
    19261 atgataccac gattgttact agactgacca gaatcatttt cggtgcttgc gaataagttg
    19321 cttgttgcgc aaagtccgta tacatgagcc acgtttccgt aaaccactcc gttagaaatt
    19381 ccaagccttt gatttttgcg aaaacaccgg gaatgaaggc cgttcccatg atataaagaa
    19441 aagaaagaag caaactgaca ctggtacttc tagtgatgaa tgtcaaagcc actggaacag
    19501 ataatgtccc taatgtcagc agaagcaata caggaaatac ttttagaaca taatttccat
    19561 agtttttgat tccctcaact tcaccaaatc cgaaagcaag tgtgtaagca agcatggcta
    19621 gtcccgtgat caaaaccacg ccaagtgttg tgatcaaaag cgtaacgaga agtttcgaca
    19681 cataataagt cattcgattc cgtcctaaag ataacgtatt gcgaatcgta ccgttttgaa
    19741 attcttcccc aatataaaag cacgcaaatg ctgcgaaagc cagaagaatc catgcagcca
    19801 ttttttctac cattaaaatc cctgttgcac cgttgatgac tgtatccgtt gcttggttta
    19861 attcattgcc gtattttgta tgcatatacc attcgttcca agaataaaaa agcggaagta
    19921 cgaccatcag tcctagtgag acccacattt tacggctctt ccatactttt attttttcag
    19981 cagttaatag attaaacatg tggattgcct ccagttaatt gtaagaaata atcttccagt
    20041 ttttgcccaa ctaggtgaat accatccaca ggaacttttg cttctaccaa tgcttgattg
    20101 atggctgcta cttggtcaat tccttcaaaa agctggataa cacggctaga ttgtacaaaa
    20161 taatccctat aaccaagaga gtcgagaacc gttactgctt gttccgtcgc cgaagtgtca
    20221 atttttataa attgacgaga ttcttgtgcc aattcttctt tcgataattc tttgatcaag
    20281 tgtccttcat gcaaaatacc atagtgagtg gcgatctgtg acaattcatc tagcaagtgg
    20341 ctagaaagca agacggtgat gccccgttct tttaccaaac gttggatgat ttctcgcatt
    20401 tctgccatcc ctgctggatc catcccattc gtcggttcat ccaaaatcaa aaattctgga
    20461 ttcgtgatca gtgcatttgc aatagctaaa cgctggcgca tacctaaaga gaaattcttt
    20521 gctttctttt tccctgtatt ttccaatcgc atcattttca gcaggtcttc gatttctcta
    20581 tccgacacac cgccgttcgc tgcttggata cgtaggttat ctcttgcagt taattctgga
    20641 taaagagcag gggtttcaat cgattgaccc atacgtcgtc ttgctgcctg caaatccgtc
    20701 gttccaaata attcgatcga accttcatca atcgagatca aacccataat agttctcata
    20761 aaggtagact tacccgctcc attttcccca atcaatccat agatcattcc acgtttgatt
    20821 tcgattgata ctttatctaa tgctttggca gcaccgtatt ttttggttat accagttgct
    20881 ttcaagaccg tttcatttcc aaaatccata cttatccttc tttcttattg atgagtgaag
    20941 tatagttgac gggtataaag aaagcagcca gaaatgtata aagaaagtgt aaattttcca
    21001 attgaatgtc aaaaaaaaca atcggacaat acgtgtcttt cctattatca ggcagtttgt
    21061 tatacgcttg tatcagtttc ctgattcttt cttttcgacc aaagtcgttt ctctgtattc
    21121 ctcttcactg aaaatttttg cttctttcag atcaagcttg agtaatttta ccagaccggt
    21181 tataaggata actatagcaa gactccctcc cagaaaaatt agactgtctc gcagacgtgg
    21241 ttgcgttgtc ttccaatcaa agagcgcacc aaacagtgct aatccaagtg gactggccac
    21301 catggtaatc gttgttatcg ttgacataag tcgtccttgt atatgcgttg gaacttctcg
    21361 ctgaagccag accatataaa aagcaaaaga aaacattaat ccttaaaaac ggacttatga
    21421 aaaataatac atgccgtttg ggatttacaa ttagctacca aataagaggt tatgacaaaa
    21481 gacttttcac aatcccttct tccgaataaa cggtgttctc ctgccaactc ctcggtcaca
    21541 agtcacaata tttatcaaac atgagaagca atttttgtaa attccttctt atttcttgaa
    21601 gctgaccact tctgtcacaa cctcagaagc ggataaataa tattgtttga tgatacttta
    21661 tctttttccc gtttgtttat agatcgaatg atattataga ccaagaatcc tacctattaa
    21721 catagctaaa aacacaaaaa aggcactttc caaattgtgg aaagtgccag aaaagttagg
    21781 attaaggtaa ctgcgctcag cattgcttcg cccagtacga ttctcgtaac tcgtgaactc
    21841 gaacgataat cgcaattaac gttttgagaa ctgtgaagct ttacgggctt tcttaagacc
    21901 tgg
//
LOCUS       DACDEG010000040        21221 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.042, whole genome shotgun sequence.
ACCESSION   DACDEG010000040 DACDEG010000000
VERSION     DACDEG010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.042"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            215..421
                     /locus_tag="IV142_001515"
     CDS             215..421
                     /locus_tag="IV142_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485180.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            617..784
                     /locus_tag="IV142_001516"
     CDS             617..784
                     /locus_tag="IV142_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP8485181.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            810..1154
                     /locus_tag="IV142_001517"
     CDS             810..1154
                     /locus_tag="IV142_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8485182.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            1159..1440
                     /locus_tag="IV142_001518"
     CDS             1159..1440
                     /locus_tag="IV142_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485183.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            1543..1857
                     /locus_tag="IV142_001519"
     CDS             1543..1857
                     /locus_tag="IV142_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP8485184.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            1835..3529
                     /locus_tag="IV142_001520"
     CDS             1835..3529
                     /locus_tag="IV142_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP8485185.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            3549..4727
                     /locus_tag="IV142_001521"
     CDS             3549..4727
                     /locus_tag="IV142_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8485186.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            4690..5376
                     /locus_tag="IV142_001522"
     CDS             4690..5376
                     /locus_tag="IV142_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP8485187.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            5376..6536
                     /locus_tag="IV142_001523"
     CDS             5376..6536
                     /locus_tag="IV142_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8485188.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            6546..7421
                     /locus_tag="IV142_001524"
     CDS             6546..7421
                     /locus_tag="IV142_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485189.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            7418..7729
                     /locus_tag="IV142_001525"
     CDS             7418..7729
                     /locus_tag="IV142_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485190.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            7719..8072
                     /locus_tag="IV142_001526"
     CDS             7719..8072
                     /locus_tag="IV142_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP8485191.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            8062..8463
                     /locus_tag="IV142_001527"
     CDS             8062..8463
                     /locus_tag="IV142_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485192.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            8456..8860
                     /locus_tag="IV142_001528"
     CDS             8456..8860
                     /locus_tag="IV142_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485193.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            8872..9477
                     /locus_tag="IV142_001529"
     CDS             8872..9477
                     /locus_tag="IV142_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8485194.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            9497..9859
                     /locus_tag="IV142_001530"
     CDS             9497..9859
                     /locus_tag="IV142_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485195.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            9862..10044
                     /locus_tag="IV142_001531"
     CDS             9862..10044
                     /locus_tag="IV142_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485196.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            10061..13492
                     /locus_tag="IV142_001532"
     CDS             10061..13492
                     /locus_tag="IV142_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP8485197.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            13543..14280
                     /locus_tag="IV142_001533"
     CDS             13543..14280
                     /locus_tag="IV142_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8485198.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            14290..16581
                     /locus_tag="IV142_001534"
     CDS             14290..16581
                     /locus_tag="IV142_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485199.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            16605..18731
                     /locus_tag="IV142_001535"
     CDS             16605..18731
                     /locus_tag="IV142_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485200.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKRSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            18894..19340
                     /locus_tag="IV142_001536"
     CDS             18894..19340
                     /locus_tag="IV142_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485201.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            19342..19479
                     /locus_tag="IV142_001537"
     CDS             19342..19479
                     /locus_tag="IV142_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP8485202.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            19517..19810
                     /locus_tag="IV142_001538"
     CDS             19517..19810
                     /locus_tag="IV142_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485203.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            19807..20031
                     /locus_tag="IV142_001539"
     CDS             19807..20031
                     /locus_tag="IV142_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP8485204.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            20028..21053
                     /locus_tag="IV142_001540"
     CDS             20028..21053
                     /locus_tag="IV142_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8485205.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
BASE COUNT         7475 a         3215 c         4454 g         6077 t
ORIGIN      
        1 gcaatgttat tttgttgctg tctattgttt tttaattatt cacacgataa ctaaaggtgg
       61 gtgaagagaa atgattccat taataatttc aatttttgcg ctctgtctta atgtctatat
      121 gattggattt aaaaatgggc aaaataaaaa atagtagcag ccaagaataa ttttatagtg
      181 tcactgtggc ggaaagggta gacgctaagc atgtgtgcta ggtcaatgct tcggcaacca
      241 tgcaaggttc gattccttgc cagcgacatt aaatgcctat gacggttacg actaccgaaa
      301 aaagatcgtt aagaagctat acggtgctac gtacggcaat gtagtaagtg tgctatctgt
      361 acaccaccaa gcttcggtca ctgtggcgga agtagaagac gcagcggtaa atggcgagta
      421 gcctcgtgag agcctggtaa gttctcgtga gtggtgcaat cccactccag cgactttaag
      481 caaccgaggc atcggcggtt taaaaatata ggggtgcgca atttcgtacg cgttttgtgc
      541 atcgtgcaag ttactattac atattagatc actctttgag tggtcttttt atttttgcac
      601 aaaggaggaa acaacaatgt atagaccaca atacttagaa cagaagtatg aagtaatcac
      661 tgttcataaa ggtaatggcg aaaaagtata tgagtataga agaccaataa agagcgatac
      721 atataaacga aaggaaaaca atgaagttat tccattgtat ggcaaaagaa tagctaagca
      781 ttaaataaga ttgcgaaagg agacggaaca tgaccgagga attctataga tggctattac
      841 agttgacaag agaagatcgt ttggttaagt tctatcagtc tcctaaatgg cgcaggctta
      901 gagagaaagc gatgaaacga gatcactatg aatgccaaga gtgtagaaga ctaggtaagt
      961 atcatagagt agagaacgtt catcatataa aggaagtcaa ggatagacct gacttagctt
     1021 tagatttaga taatcttatt tgtttatgtg ttgaacatca taatgaagtt catggcagat
     1081 atcttacagc attagataaa caagagaaga agatagaaag ctttgctaac ttcgatgcaa
     1141 gtgaaaggtg gtaagtgcat gatcatcaat gataatggca gagagtatga tacagaaaag
     1201 attgaagagt attcatctta tactcaggga ttaattaaac gtttgatata cgttcgctat
     1261 gtaggtatta gggatctgtt atcagataac tgttgtagta aatacaaagt gaatcaagta
     1321 agagaagcgt tgaataaaga taataacgtc gaaagaataa aaaatgtttt tggatatagt
     1381 attgaagaga ttaattatta cattgacttc gctgaagctt tcattccgat ggtgagataa
     1441 ccccccctta aaataaatcg caaatttttt gggggtgatg aaacggaggg ggctgtcagg
     1501 aaaagagatt ttttcgaact ttatcatgaa aggagggcta aaatgtttaa aaacgaattg
     1561 tctcaaaatc gctacagaga aaaattacgc cgctctttaa taagccaatt ggaaagtcag
     1621 aaaacaaata ttgagccatt cttagataat gttgatcgtt atatcagttt atgggaaacg
     1681 gcgatatcac tggaagaaga tatatccgag aacggcatta gactggagaa tggtaaaaag
     1741 aatgaatcag tagcgttgct tgtttctgtc aacaaacaaa tgggattgat gttggataaa
     1801 cttgccatta ctcctgaatt ggtaggtgaa gcaaatgaat caattcctga gttataagca
     1861 tattgaaaat tggttcaaag ctatagaaga aggcactatc aaggtatgca aagagcaatt
     1921 attgctaaaa aattatctag aagaaagagt ctttactaga gaagatattt acttcgataa
     1981 gcagatggta gaggattcaa tcaatatacc agcacaatac tttccattcg aattaattcc
     2041 gtgggaaaaa tttctacaat gttttattta tggtgttcga tggaaaaaag ataaaacact
     2101 agtgttcaat agatatcttt cattaatggg acgtggtaat ggtaaaactg gttttgcttc
     2161 ttggaacaac ttctttctac taaccgctaa acacggtatt aaaaattatg atattgatat
     2221 ctatgccaat aatgaaagcc aagcaaagac tagttttgat gatgtattta aagtaattaa
     2281 agatcatcct gatttagata aaaaagtatt taaagctacg aaggaagtta ttcaaaatat
     2341 cgctacaaac agcaaacttc gttataacac ggcaaatgct agaacaaaag atgggaagcg
     2401 accaggtgca aaccgctttg atgaaattca cgaaaatgaa gattattcaa tgataaatgt
     2461 ggctacttct ggtggtggta aaattcgaga ttatagagaa ttttatgata caactaatgg
     2521 tcatgttcgt ggtggtccgc ttgatgacat tatagaagaa tcaaaaatga ttctttctgg
     2581 agaacttgga attgacaagg atggagcaga attttctagt ttgtttccat ttatttgtcg
     2641 cttggataac gataatgaag ttgatgatcc cgacatgtgg gaaaaagctt gtccaactat
     2701 taattacaat gcagatttaa aacggaaaat gtttcaagaa tactctcaaa tgcaacgtaa
     2761 tgctggttta agacttacgt tcatgaccaa acgaatgaac agacctatgg aagatacacg
     2821 atttgctgtt gcttcatatg atgatgttct gcatacgaaa gaaaaagaat ttcctgaaaa
     2881 aatggatgaa gtgataggaa cagtcgattt tgctgataga cgagattttg ccagcgttgg
     2941 gttgctagga aaatacgata aagatgttta ttttacacaa catactttta tccacgaatc
     3001 agcccttcga ttacaaaaca tcaaacgaga ggttatagat atttctatag atcaaggaaa
     3061 atcacagatc gttcatggaa aaaatataga agctgattat attgtaggtt ggtttcttga
     3121 aatgagtaat aaatattata ttaaaaaaat cgctatggat atgtaccgtg caaaaatatt
     3181 gaagcccgct ttagaagaag caggttttac tgtggaaatt gttcgaagcg gatctgttac
     3241 acatggtatg ttaaaagatc tggttgatga cctttttatt aatcaacgtt tattttttgg
     3301 tgacgatgcg attatgcgtt ggtattgcat gaatgtatat gaagagcata tttctaatgg
     3361 aaatatacgc tatgaaaaaa tagaacctga aactagaaaa acggatggct ttttttcatt
     3421 ccttcatggt ttgaattttt tagatgatat ttatgattct gctcctgtaa cagtcacaaa
     3481 tagctcagta gaaaatacag gaactggatt tactcctcta gtattctaac ttgaaaggag
     3541 gtgagaaagt ggggattttt caaaaggcgg taggatactt cacaaaaaaa gcaacggttc
     3601 ctttagaaga atacttttgt aaattgcaag ttgattttgt gtatcgaaaa tttgcgattg
     3661 aaacttgtat tgatttgatt gcaaatgcga tgagtaaagc ggaattcaag tcatatgaag
     3721 atggaaaaaa taaaaagaat gatctttact ataggctgaa tgtagctcct aataagaaaa
     3781 ataatgcaac agaatttaga aaaaaactga tcaggagatt aatattctac aatgaagtat
     3841 tgatcgtttc tccgtctaat aattctagcg aaatatttat tgcggatagt tgggatgtca
     3901 cagaatatgc attgaaagat gatgtgtttt ctcaagtgca aattaacaac atagtccttg
     3961 atagagaatt tctagaaagt gatgttatct atataaaata cgcagatcaa caaattaggc
     4021 aactagtcga tgcgtattat caagcgtatg ggaaactcat ttctagtgcc atgaatgttt
     4081 acaagcgctc taacgctcgt agatacgtac tgaaagggaa tttattccga ccgcaagaca
     4141 atacaacaca agatcaaatc aataaaatga tgacatcaca atttaaggct tttatggaag
     4201 ctgataatgc aggtgcggta tttcaattac aaaatgagta cacattagaa gatttcagcg
     4261 gaaacttcca aagcaattca agagatataa aaaacttaat agacgacatc tttgagatga
     4321 cagcagcagc gtttcacgtt ccgaaaaacc tactaaaggg agacatgagt gggttatcgg
     4381 atcaagtgga cgctttttta atgttcgaaa tcataccgat tgctgaactt attcaggatg
     4441 cgtttaacgc tagtctctat gaagtagaag aatacttgtc agggaatttt gtacgtgtgg
     4501 atacaactat gatcaagatt actagcttca aagatttggt tgacgctatt gatgtaggca
     4561 ttagaaatgg agtatttaca atcaacgaag gaagagaacg cgttggaaat gatcgctctg
     4621 ataaggcgat ggcagatgaa atatttataa ctaaaaacaa tcaacaagta tcgaaaggag
     4681 gtgaggcgaa tgacgacaat gaaaacattt ctagcagtaa agaatgaagg cgcagtaccg
     4741 caaattttta ttcagggatt tattggttct agttggttct ttgaagggaa tactgacaag
     4801 ggaatcaaaa atattttgga tagtctaggt gatcaagaag aaattgaagt agtaattaat
     4861 tcaaacggtg gagacgtatt tcaagggatt gctattggga acttacttaa gtcaaataaa
     4921 gcaaaagtta acgttgtgat taacggctta gccgctagtg ctgcttcaat tatcgcaatg
     4981 gctggcgata ctataaaaat ctacaacaat gcacaattga tgattcaccg cgcttccaca
     5041 tacggagaag gaaatgtcga tgacttccgt acgattgctg accaactgga atcaattgat
     5101 aaatcggtaa aggcttcata taaaacacga ttcaatggca cagatgaagc attgcaagaa
     5161 cttcttgaaa aagaatcgtt tatggatgca gaaacagctt tgagttatgg attggtcgat
     5221 gaaattatcg atgcagaaaa tagctcaggt actgaagcta aaaaagaaca aagcgttgaa
     5281 gaaattttga atgacgttaa agaaaaaaga gcagaaaaaa ttgctgcatt tacagcagca
     5341 ttaaataaaa catttggaca aggagatgta aaataatgac agttaaaaat ttaaaaggtg
     5401 taacagctgc aagcgaccaa ttgatgaaag cttttaaaga tggtaacgaa gaatctttta
     5461 gcgcagctat ggtaagctta tctaaggaaa ttcaggataa aattttagaa gaagcaacag
     5521 caaaaaatca agatcaatta gtattaatga accgtggtca gcgtgtatta actacacaag
     5581 aaacaaaatt ctataacgaa gtagtgaaaa acgaaggttt tgcaggggtc gaagaattag
     5641 tgccagctac tgtatttgaa cgtgtatttg aagatttaga acaatctcat ccactattgc
     5701 aaaaaattac ttttgttaac acaactggtg taacagaatg gattgtgtca cgtggagtca
     5761 atccagcatg gtggggtaaa ctttgcgaag ctgttaaaaa agttttagat aatggctttg
     5821 atgtaattaa catgaagcag ttcaagctat caggttatat tcctgtatgt aaggcaatgc
     5881 ttgatttagg tccagtatgg ttagatcgtt atgtccgtac tgttttagta gaatcattga
     5941 gaattgcatt agaacaagca attgttgatg gtactggtaa agatatgcca gtcggaatga
     6001 tgcgtgacat gagcaaacaa actagcggag aatatgctga aaaaaaagca gaacctatta
     6061 cagctttaga tgctgtaact atgggcggtt tgatggcgcg actatcaaaa ttcaatatcg
     6121 aaggtgtgaa tgatccgatt tatcgtaatg tgaatccttc tgatgtggtc ctaattgtga
     6181 atccaactga ttactggtct aaagtattcc cagctaagac tgtactaact gctaatggag
     6241 aatacgtaca agtattgcca gtaccagttt cagatttgca gtcaacggct gtgccagaag
     6301 gaaaagcagt tattggggta gcctcagatt acttcatggg tgtaggatct acactaaaaa
     6361 ttgaagcttc agatgaatac cattttgttg aagacgaacg catttatcta gctaaacaat
     6421 atgcaaacgg tcaacctaaa cgtaacgata gtttcattgt attagatatt agcgctttgg
     6481 gaactactac tacaactaca aaaccaacaa ccacaacaac tacaacacaa gcgtaggtga
     6541 tcagaatgaa gtatattctt tgtcagccgg caatcaatcg gtttaaatgg gagcttgaag
     6601 tttgtttaac taatctgaag aaactaggaa tcaaagatat cgtattgctt ttcagcagac
     6661 acgatgatca gattcctatt ttttttgaga aggaatatgg tgttgaagtt catgtgtacg
     6721 atgatctgcg ggacgacaaa gagtatattc cttcgattaa accatattta tggtggaaat
     6781 atttagaaga agatcattcg cgtgaggacg accgatattt ctatatcgat tcggatgtca
     6841 ttttcaataa aagaattaat ttgcgcaaat tgccttctaa agatgatgtt tggtattgta
     6901 gcgactgctg tagttatcta agtcttgatt atattagaag ctgtgaaaac ggagaaaata
     6961 ttctaaaaga tatggcaaac attgtaaatg ttacagtaga atctttggaa actataaaca
     7021 ctaattcagg aggcgcacag tgggttatta accgccctaa agcgaattat tggaaaaagg
     7081 tttatctgga ttctaatcgg ctatatcgct accttagagg gcaaaaaaca aatatacaaa
     7141 tctggacagc cgagatgtgg gcacagcttt ggaacatgat gtatttcaat attggtccta
     7201 aagttcacga ggaattagac ttttgttttg ctactgatcc aatagaaaaa gttaaagaag
     7261 taaaaatctt acacaatgct ggagtaacaa caaacgatga agatttattt ttcaaaggga
     7321 gatacgtgac ttctacgcct tttgatgaag atttatcatt tgtaaacaag aaaaaatgct
     7381 cttacgcata tgttaaagca attaaggcgg tggttagatg acgcctgaac aagtgactga
     7441 agaattgcta atagctgtga aggataatat ttacgttacc tggaacgaag aagatgagtc
     7501 aattaaaaag atgatagcta aaaatgctgt ttatcttcaa agtaaagtga gtacaacact
     7561 ttctttttct cctgaaagct tagaatacgg attgctaatc gaaagatgta gatacgactg
     7621 gaatcgtgct ttagatgagt ttgaacaaaa tttcgctagt gagttattag gtttcattca
     7681 acattatgcg ctacaagaat atattgcagg tgatgtgaat ggcgaataat cgtagactcg
     7741 aagaaacatt caacgatggt tggttaaaga ttttgacgca aactaccaaa agaaatgaac
     7801 taggaaaaaa gattggtgta gaagatacag aaatcacttc tttaaaattt agaaatcttt
     7861 ccatgagaga tagtgatata acagctatgg atgcgatggg atcgaaatta actaagaaag
     7921 taaagactcc atttcatcca atcgccaaga aatttaataa agatcaatat tttatcgtaa
     7981 tcgatagtat gcgttacaac gttatctatg ccgattacga taatttttat atctattttt
     8041 atcttgaaag tgtgggtgaa tatggtgatt gataattcta aagaaaaaga acgtttaaat
     8101 aagcaaattt ctgctatcaa aacttcctta gaagagcatt ttggcctcaa actctttcaa
     8161 gactccgtag gcgaggatga gctacctgat gattttaatt acttcattct cgaaacagga
     8221 gaaatagaaa tgatcactga gccaaaatat agcgtgggtc aaaatctata tctaactttc
     8281 tattcagaaa atagagaaga tttaacagga gattcactag atattatttc attgattcaa
     8341 aatcgttcga ttcgttttca gagaatggat cccaatcatt taaaactaga gaaccaagat
     8401 cgctatatcg atcaattggt atttacgttt agacgattat tgaagagtga ttgtcatggc
     8461 taaaaatagt tgggagctaa aaataaatgg acatgatgaa cttcttgtgc ggatggaacg
     8521 ctattcaagc gagagcgaac gactgattaa cgaagcattg aaatcaaaag gttcggctat
     8581 tgcagtggat aggattacag aaaaaattcc tgtttctgaa gcagatttaa gaagagggca
     8641 ccaacacgca aaaaatagtc gtccacttaa gactcaatac attaatttgg gtttcatcat
     8701 tagacctaca agaaaatttg agtatttaaa atatcctgat ttggggatag gtacttctaa
     8761 aagaaatcag ccggacgaat ttatgagaag aggattaggt cttgcacttg atccaattac
     8821 agaacttctg attcgtcaat tcgataaatt aaataaatag gaggaacaac aatggctaaa
     8881 acaacaacag taacaacttt tgacaatata agtattaaaa gaatttcttt taattttaag
     8941 aatgcaacaa atgcaatttc aactgattgt aatggacaat tagatggcga aacagaaatg
     9001 cagagaattg taaaaaaatg cggttcaaca gaagtaaaat cgaaatctaa accaatcaat
     9061 atgacggtaa caattactgc acatgtaccg atggaagttt atcgacgctt caatgggttg
     9121 aaacaagatg aacgtattaa atcgggaatt tactcttatg gtcctgattc cgtaggggaa
     9181 gatttctctc ttgctgcaga gatcgtggat gatttcgaag aaaaaaataa gctgattggt
     9241 atgttagcat gcacttcgaa tacaggatta acattctcta ttgaaaatgg tgcggatgaa
     9301 gtagctgcgt tagaactaga aacaaaagtt atgcaagatg aatttggtaa attttatcat
     9361 gaagcaattg ttgcagaact tgaagaagac ttaacagatc aatggatgac gaatctatct
     9421 gctgatgtga ttaaaaaaac ttcagttgtg acaactacgg ccactcaatc acagtaaaaa
     9481 aaacggaggt agcgaaatga acgaagatta ctcaaaaatt gaactaaacg atggaacaat
     9541 tttgaattta gaacctaaac tgaatatcaa gaaattattg atgatcaata gagattttaa
     9601 cacagacgag tttgcaaaaa tgactgtggg aaaaggatcc atggatattt ctgttattca
     9661 aggtgcaaag gctgtgtata ttgcttaccg ccaagcgaac atgactgatt atatttcatt
     9721 cgatgaattt atcgataaat gggattttga tatggctact gccagctata tttatcaatt
     9781 gatgatgttc aaacaagcac gcgatgctta tcaaaaagaa tttgaaaaag caaataagga
     9841 aaaaaagctt caaaagtaaa aatgccaaag ctcttagttg aaacgtgggt cgatgtctat
     9901 tcgatgttga ccgacgtttt ttctatgcct tcagatttgg ttttaagcga tatctgttta
     9961 gatgacattt tgcaaatggc ttacaacaag agtgcttatg aaggatggaa aaactatgca
    10021 ataaaccaat cccagaaaaa ctaaagaaag gaggtaaaaa atggctaaaa agagaacaga
    10081 agcagaagta acattcatag ctaacgatga cggattgaaa tctacgttaa aagaaatcag
    10141 tgctgaatta actaaaaata gagcagaatt aaaactagaa caagctcaat tacaacagac
    10201 tggttctgaa tcagacaagt taggaagtaa attatcttct ttagaaaagc agtatgaatt
    10261 acaaagtcaa aaagttgaag taactagcca acgtttagcc aatgccaaaa aatattatgg
    10321 agaaaattcc accgaagttc agaaacttga gagagaactg attaaccaac aaacagcgca
    10381 acaacgtttg tcaaacgaaa ttgataaaac gagtaatgca ctagctcaag caaaaggcga
    10441 aatacagacg tacgagtcta caatgcaaca gttggatagt gaacaaaaaa atgttcaagc
    10501 tagtgcttct ctgattgaat ccgaatacaa aaaatggcaa gcaactgctg gtcaatcagc
    10561 ttctgaatcc gagaaattag cgaaagccca agaatatgtt tctcaacaat ctgaaaatgc
    10621 agagaaaacg atagatatcc tgagacgaca gttagaagct acacagtctg agtttggcgc
    10681 tacatccaca gaagcaatgc agatggaggc gaagcttaat gatgctgaac gtgaatttga
    10741 agagttagga caagctgcta aaaatgtaga tacaactaac ttggacgata tcggaagcaa
    10801 aatagatatg aataatctaa tggaagcttc tgacgtttta agcgacattg gcgataagct
    10861 tacagaatta gggaaacaag cagtggactc tgctaatagt gtaggtagtt cccagagtaa
    10921 aatacaagct aattttggtt tgactaaaca agaggctgaa gaattaacga atgtagccag
    10981 agacatttat tataaaggtt ttggagaatc gttagatcag tccacagatg cattgatttt
    11041 ggtaaagcgt aatttaggcg atttaaataa tcaagattta caaaatatca cggaacaagc
    11101 tatggtccta gaaaacacca tgggcgctga tatggatgaa acgttacgtg gtgtaaatgg
    11161 cttaatggtc aatttcggct tgagcgctca agatgcaatg gatttaatgg tttcgggtac
    11221 tcaaaacggt ttagataaaa cgcacgaatt aggcgacaat atggcagaat atagccaatt
    11281 atggagtcaa atgggatatt cagctgatga aacgttcgga atgcttcaaa atggtttaga
    11341 tgcgggtgct tataaccttg ataaagtcaa tgacttagtt aaggaaatgg gaatatcgtt
    11401 aacagatggt cgatttgagc aaaacatgga tatgtttagt gaaagtacta gaaaagcttt
    11461 tgaagagtgg aaaaatggcg gaggaacaca aaaagacgtt attaattcca tgattcaaga
    11521 ttttagcaat atggatggtc aatacgacca attaaataaa gcttctacaa tttggtctgc
    11581 acttggcgaa gataacgcga tgaaagttgt ccaatctttg actgatgtta accatacatt
    11641 tgatgatgtt agtggatctg cacaaaaaat gaatgaagat tctactactc cgttacaaga
    11701 attaaacgga aaaatagctg aattaaagga ttcattagct cctataggca acacaatcat
    11761 agatgcactc gaaccagtaa ttgattttct aggaaagatg gctgatgcgt ttaataatct
    11821 tccacaacca gtacaggatt tcattgttgc tataggtggt cttacagcag catttggcat
    11881 actgttaccg gcaatactag ccgtgtcata tttatttggt ccgatgatgc ttataattgg
    11941 aggaattata gctgttatag caggcgttat tgtggcaatt aagaactggg gtgcaattac
    12001 tgactggttt agtggtttat ggaaaaaatt tactgattgg ttgggtgata cttgggaaag
    12061 tataaaagac ggagcctcat cagtttggga tggggttaaa gaaacctggt ctggatttgt
    12121 agattgggtt caagatattt ggcaaggagt ttctgattgg tttggagagc tatggagcgg
    12181 attagttgaa ggagcttcca acatctggca aggagtccaa gaaacttggc aaacattcgt
    12241 tgattgggtt tcaaatattt ggaacggagt caaagaagta tggtcgatta tttgggcaga
    12301 cattgtagga attgttcaaa taccatggac attaataacg tcattgattc aagctggtat
    12361 taatattatc gtgggtattt ttgatgtagc tggacagtta ttgggcgcag cttggcaagc
    12421 tgtttggaca cctatttctg atttccttaa aaatacttgg gatactatga cacaatggat
    12481 aagtatcgct tggaatggga ttgtaactac attccatact atatttgatc cagtagtggc
    12541 atggtggaat ggtatatgga cagctattag tactacggct tcaaatattt ggaattcaat
    12601 tagtgcaaca gcttctagta tttggaacag tatcaagaat acaatcacta gcttggtaca
    12661 agcagctgct acagtaattc aaaatatttg gtcaactgta tctagttggt taggtggaat
    12721 ttggaattca atcagctcta cagcatcaaa tatctggaat agcgtgacta gtagtataag
    12781 caatgctata aacgcagcta aaagtgccat tcaaagtgtt tggaatagta tatcttcgtg
    12841 gattagcgga atttggaacg gtatcaaaaa tactgctttg aatctttgga atggaattac
    12901 aagcactatt agctctaaag taaacgatgg aaaaaatgca atttcaagcg gttggtccaa
    12961 tctaacaggt attgtttccg acatattcaa taatgttaaa agtacaattg ctaacatatg
    13021 ggaaggcatc aaaaagactg ttagcgctcc aattgattgg attagagaca aaatcagtgg
    13081 catttttgat aatttgaata tttctatacc acatattccg ttaccggaat ttatcatgga
    13141 aggcagcttt aacccgctaa aaggtcaaat cccccatttg cgtgtaaaat ggcatgctaa
    13201 aggaggtatc tttactaaac caactttact aggtggaatg aacggtgtcg gtgaagcagg
    13261 acctgaagca gttttacctt tgaaaagatc tgttttgcaa gaaattggtg atcgtatctt
    13321 gagtagcaca tcagtttcat ctagggcaca aacgattcaa cctgtgaata actacgaatt
    13381 caatttcaca attgatggta acgcagatga ggttactatg aagcaaacaa ctcaacaaat
    13441 cattgatagc attacaaaaa ttcaaaatga taatgcttcg gcatggcgtt aaacaggaga
    13501 gtatttctcc tgttttttta gtattaaaaa ggatgtgaaa aaatgactga ttgtatacat
    13561 tctataatcg atggatttcc tgattatttg cataaattgg ctttagctga aagaccaacc
    13621 ataccttctc caaaaagaca gagagttgaa acttctgttt taggtaggtt aggtggctta
    13681 gtacaagatt actcgtttga agatatgtcg tttacattgc actataacta tttagaggat
    13741 gtggaagacc atcaagcgtt caagcaatcg ttttatatca tgcgtcattg gttaaactat
    13801 gcaaagaaat tagaattctc tgacgatccc aacgtctatt acgttatcca gactatcgat
    13861 attggggatg cagaaaacga tattgttgaa tggggagagt tcgatgtaaa tattactgcg
    13921 aaaccattcg caagagttca agaagatgta cctataaccg tagataaacc acagtcattt
    13981 aacttgctga ataatagttt agaagaaagt tttccaaaga ttatcatcac tccttcagct
    14041 acttcatgcc agttcatctt aaatgattat gtgtttagtt tcgaaggctt agtagcagga
    14101 actgatgtag tcattgatag tgatttgatg ctttgctacg aagagcaatc ggacggagat
    14161 attttagatc ggtccaacaa aatgaagacc atgcaatatc cgacattgca agtggatatc
    14221 aatcatttta attgcactgg tttgagcaaa atacaaattt atcgtaacgg gttaaggtag
    14281 gtgaaataga tgatcgataa tttaataact atttacgata aaaatgacgc gaataattta
    14341 gctgaacatt tatatgatac gcaaggttta ggcgctttgt cagactggtt aacagctact
    14401 gttagcaata aactaaacgg agccgagata tttcagggta cttattcaat aaacggaact
    14461 aatgcagatt tgattgtaga cggacgtatt attcagtgtt atgtagatga aaatcgagca
    14521 aaacagcgtc tacggattta ttatgcaaaa acttctgtaa taggcaatac gatagaagta
    14581 aaagctgaac ctattttcaa tgatataaga aaatcggtgt tgaataaata tgacagcgga
    14641 acagaaaaga tcactgctac tcaggcatgg caaaacgcaa aagttttagc gaaaccagct
    14701 atcccttcgc agttttcttt ctcgtcatta gtagatacgc ttgctaatgt gaagatagaa
    14761 aaggcgaatt ttttagaatt ctttggtgga aaagagggat ctattctaga tcgatttcat
    14821 ggtgagtttc taaaagataa taacacatta cgtcatgaaa aaagtctagg tacggatcat
    14881 aaaatcaaag cgatttatac taaaaactta actggtcttg acttagagat tgatgctcaa
    14941 agtgttttag ttggagttta tccattcatt agcagctctt cagaaggaga agacgagatc
    15001 actctaccag aagaagttat tttcacggat tacgtggatg attatcctgc tggatatgtt
    15061 tcttttgttg attttaaaga caaagcgact gatgtagcca cattaaggga agctgctaaa
    15121 gactggttga aaacaaacat agataaacaa aaaccacaag tgagtggttc gattgaatta
    15181 gtaccattga ggcatcaaag aggctatgaa aaatttgttg atctagaaaa agtttcgatg
    15241 ggtgacggag tagatgtgta tcatccacag ttaaaagtga atatgtcagc gagaattgtg
    15301 gaatatacgt ttaatgttct aaccaattca tacgataaat tagttgtagg aaacgtcaaa
    15361 acaaacttct tagaaaacac agagaataat gtcagcaatt tgattaatga tgccattgat
    15421 caattgaaaa acggtggcga aatcagtgat ttactcaatg atattgtaga tcatcaaact
    15481 gatatgatta ctggtcaaaa tggtggttat gttttattag atcctaaaga agcgcctagt
    15541 cgtattttga ttatggacac accagataag aataccgcaa ggaatgtttt acaaatcaac
    15601 aatgctggta ttggtttttc taaaactggc attaatggaa catatgaaac ggcatggacg
    15661 ttagatggcg gattcaatgc ctcgtttatt acggctggtg aaatagtagg aattactatt
    15721 agaggtacta cattaattag tgatggtgct gattatagaa caagtattgc taatggcaaa
    15781 atgacttggt actcaaaaaa agttaacaaa gatattatgg agctagaagc acgtgattat
    15841 gtaagtgctg atgccggtat tgtatcatac accatgaaaa ctggtggtgg tttcatgatt
    15901 agaaatccac agggtaactt ggtttttagt acgtgggata atggtaataa cagaccgttt
    15961 ctatcttttg gtgcgcccaa tttcaggtat agcaatgcta gttatgtaac ttctggcgac
    16021 ggtagttctt taagcattaa tggtagtgcg ggtaactcat gggaatttaa ggtagctggt
    16081 aggactatga aatttactag tgatggtatg ctaacgttac caggttgttt ttttggttca
    16141 tgggaagatg ggaaacttgc taggtttgaa caatcaacgg tacaagtata taaagatttt
    16201 actgttagag gtactaaaaa ctcaactgta ccaacagaac attatggaca acgactattg
    16261 aacgcttatg aaactccaga atattatttc gctgattatg gggaagccgt tacaggtgac
    16321 aatggtaaag ttcgtgttga tattgacccc atgtttgctg aaacagtaaa tctaagtcgg
    16381 tatatgacac atgtgacacc tacagaacta gttttgtgtg ctgttactca tgaagatatt
    16441 gaccatttca tcattgaaac tagtaagcca aacgtattag ttagatggaa tttagtggca
    16501 caccgtctag ggtatgaaga tattagatta aaagaggata cagcatatga tagcacagtg
    16561 cttgaccaaa aacgttttta aaacgaagac aaggaggtat ataaatggct agcagtttat
    16621 ataatttggc tttagatttc agcaaagaat taaactacac caaagctatt atggctcgtc
    16681 aaggtgataa agggattacg gtgacggtta aaccgtttct aaatggcttg cagatggata
    16741 cgagtggcgg aacatttact ttaaaaggaa caacaccatc taaccgttac gtagataatg
    16801 ttgcaactag tgtaactagt gaagaagtca cgttttctct tgatggcaca tttatgagtg
    16861 aagcaggata ttataaacac tgctacgtag aatatagaaa agacaatcaa attttaacaa
    16921 cgcaagatat catttttttc tcactaggag tgtctgacat ttcgcaaggc caagccgatg
    16981 aatatgtttc gcaattagaa gagttgattc gaaagtacaa cgaaactttt gatgctttta
    17041 tggctgaaat caaaggtaga gtggatagct taaatcaaca gattactgat ttaactggtc
    17101 aagctaaaac gctacaagac aagttagatg ctctgaaaga agaaatttct aagttaggta
    17161 acttacaagt gatgtacagt aacagcatcg acttcgggga ctatgattat tcggagaacc
    17221 cgaatttaat gccatatatt acagaacctt gggttggacc tttgttaggt aatggacata
    17281 cagttaaaga tagtgttaaa agggttataa ctcatactaa aactagaaca gctaattccg
    17341 gcgacatatt atcgcttggc ttaggtattc cgtgtacagc ggaagctaat aataggtatc
    17401 ttataaccac tttacgacca tctacgacat acacattaag cgttactatg agtgtcggtt
    17461 cagattggac tggagagacc aatactatag gggttaggct tagatattta aatgaacaag
    17521 gtggtataga actgcccatt aatgcattaa taccagcaaa tgtagaacgc gataaaatgg
    17581 tcactcatac atttactggt ataacaaaag ataatgtaac tagcataaca aactgttatg
    17641 ttgaaatatt ctcacttaat agtgaatata aaggaacggt tagtgtcagc tatgacgtta
    17701 agttaaaagc acactaccct aacttactag atggccctta ctggttaggt aaagttcctt
    17761 taggcgagaa tattgctgac cctacagttg tctttccaca caaaactagt gaatatatgg
    17821 tttatggtag acgcaataca gaaaactata tcgctgacca aacatatacc atatcaatga
    17881 aagcaactaa actaacagtt caatcgtttg ccgtgtatat tgctgctggc cgcgttaaag
    17941 taggagacat gaagcctact gaggggttag ctaatacatg ggagttaact tttactgtaa
    18001 ctaagcaaca tatagatagt ggggtaacta attatctgga aatataccaa tacccgtcag
    18061 ctacaaaagg agctgttcaa attgagtggc tcaaactaga aaaaggcaat actcgaaccc
    18121 cgaatattag tgagtataaa tatcgtggta ctggtatgcg tgattcaaac aatccaaaag
    18181 attatgtttg ggatctagca ccagaatatg tcgaagataa tttggccaca gatattaaaa
    18241 tttctgaaat tactggtaaa gcaaacaatt ataccgatgg gaaagtatcg gagattaatt
    18301 cgcagttgac tgcttcaatt aatgaagtag acaccacagc taaggatgct caaacaaaag
    18361 cgaatgctaa tgcgactgct atagatgaat tagacaataa gatcgatgaa cgcattaatg
    18421 atacagctac taccacatta acagttacaa acgggaatac cggatcagca aagctttatc
    18481 gtgaaggaaa aacagtttct atatattttg tggctttaaa cggaaaacgc agtggtggaa
    18541 atgattcaac gatactaaca attccagaag gctatcggcc accaattagt tttgagcaac
    18601 tggttggctc gatagaccgt tctactttga acagtgctca gttatctatt ggtgcagatg
    18661 gagccattaa atggcgaaga aactcaagtt atggatcgga ttataccttt gcaattactt
    18721 acacgattta gaaaagcgtg aatcgatatg aaggcagcat atagaccaat tgaaccttac
    18781 ggattcgagc aaatcattgt gaatgatgaa gaacatttac cggaagaatg cacagaagtc
    18841 gaaccaccga ttccaaattg gaaaccgaaa ttcaattact gggagggaaa taaatgaaaa
    18901 acatttggaa atatggacgt actggcggag agtacgcagg aaaagtattg gacgacatgc
    18961 ttgtatccgt tccttacaca gatcagccac cgcttgaagg aattcgtgct gatggcgaac
    19021 cgttaacaat cgctgatcaa atgtttgatc ctaaattgaa tcaatggatt attttagcga
    19081 acgcactaga tcacaacgat ttaaacaatc tcaaagcaat gtatgagtcg ttagaaaatg
    19141 agaacggcga tttaaaacag atcaatgcca aactcatgct aagcgatgta gcaattaaac
    19201 aggaaaatac tgcattgaaa gaaaaagcgg atagtttagc acaaatcaat tcaaaaatga
    19261 tgcttgcttc gttacaaaat agcaaagaca tttcagaaat taaagagcaa ctaaatccag
    19321 cttcaaaggg aggtgagtag tatgtttagt tttagcgatg tgaaaatgat gtatgattgg
    19381 ggctgtttca ctaacgaaca agtaatggtt ttcgttccgt tgtgcattac tgaagaaaaa
    19441 gcagataaaa tcattagcaa agaagagagc gcatcttaat tgatgtgctt tttattttga
    19501 ttcaaggagt tgtcacatga ttaatttagg ggaatgggga gcgatagcag gatcaataac
    19561 cgctatcgtt tctttgattt tattagtaat aaaaccaatt actgcatctt tctcgaagat
    19621 tactgagact ctttcaaaag taagtcacaa tttagatttg ctgactaaag atttagaatc
    19681 gagcaaatca gatcgattga tgattcatga agaactaaag aaacacgatg aaagattaga
    19741 tacacatgca gaaaaattgg tagaacacac gcaacaaatt aaaactttat ttagggagag
    19801 aagaaaatga ataataaaac gttcgaagta ctaaaatggt tcgcactggt aattattccc
    19861 gcactagcta ctttcgtggg gttagttggt aaagcgctca attggcagta cacagatatc
    19921 tgtgttgtca tcattactgg ttttggcgcg tttttaggga gtgtgttggg tgtatcaaat
    19981 cgaacctaca aaatgttctc ggctgaaagc gaagaaggag gaaacaaatg aaaaagaaaa
    20041 ttactattac tgcgatgagc ctgttaacgg ctcttttttt attacccatt aatacgtttg
    20101 cttatactat taatgacgag tataatttag cgccgaatca aggagactcc agattagcaa
    20161 ttcctaacaa aattattttg cacgaaactg gaatagatgc accagcaaga aacgtagccg
    20221 ccaacatgaa aaataattat aacggaagca attcttatac tacagatgtt attggtgacg
    20281 gtgggattgt ttaccgtgtg ggtgagcaag gatatgtttc gtggggagct ggtaacgcta
    20341 atccttatgc gcctgtacag attgaattac agcgcacata tgataaagca ttgtttgaaa
    20401 aaaactatcg agcttatatt gaatatacaa gagatagtgc aaaaaaatat ggaattccat
    20461 tgactcttga tcaaggaact tctttattta caaaaggaat catttctcat ttgtgggtga
    20521 caaattatgt ttgggggaac cacacagatc catatggtta cttatcgcaa atgggagtta
    20581 gcaaagaaaa gcttgcttat gatttagctc atggatttac cgatgaaaat ccaacaactt
    20641 ctgaaaacaa gcctgtcatt gatccaacac gagctggtgc agctaatcct acgctgacag
    20701 atggaacaaa tcacgcccac attgatcagt ttggggaaat cgaaaacgca aacttgcatg
    20761 tggctggatg gcacattgct aactataaat acgagtatat tttcattatg gactacaata
    20821 ctgggaaaga attagctcga gtaagagctg atggaattta tagaccagat gtaaaccaag
    20881 cttataatac ttctggaaat gttggttatc atgtatcttt caatatgcgt aattttccta
    20941 gtaagaaagt ctatgtaatg atgcgggcaa cgaatgatcc agagggaaac actaaaggcg
    21001 gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga taaaaatagc
    21061 tcctcgttga ggagcagtac ataactatat tgacaactat aaaaattatt cgataaaata
    21121 gtgatgttat cgcatatctt cactatcacc cataaatagt cacactccaa gctatgcgat
    21181 aacaggtttg ttgccacaca ttctactggt tgattgttta t
//
LOCUS       DACDEG010000041        20832 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.043, whole genome shotgun sequence.
ACCESSION   DACDEG010000041 DACDEG010000000
VERSION     DACDEG010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.043"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(160..1368)
                     /locus_tag="IV142_001541"
     CDS             complement(160..1368)
                     /locus_tag="IV142_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8485206.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(1387..1665)
                     /locus_tag="IV142_001542"
     CDS             complement(1387..1665)
                     /locus_tag="IV142_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485207.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1858..2514
                     /locus_tag="IV142_001543"
     CDS             1858..2514
                     /locus_tag="IV142_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8485208.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(2956..3753)
                     /locus_tag="IV142_001544"
     CDS             complement(2956..3753)
                     /locus_tag="IV142_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP8485209.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(3750..4934)
                     /locus_tag="IV142_001545"
     CDS             complement(3750..4934)
                     /locus_tag="IV142_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP8485210.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(4959..6206)
                     /locus_tag="IV142_001546"
     CDS             complement(4959..6206)
                     /locus_tag="IV142_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP8485211.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            6421..8166
                     /gene="dnaX"
                     /locus_tag="IV142_001547"
     CDS             6421..8166
                     /gene="dnaX"
                     /locus_tag="IV142_001547"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP8485212.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            8186..8500
                     /locus_tag="IV142_001548"
     CDS             8186..8500
                     /locus_tag="IV142_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP8485213.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            8525..9121
                     /gene="recR"
                     /locus_tag="IV142_001549"
     CDS             8525..9121
                     /gene="recR"
                     /locus_tag="IV142_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP8485214.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            9185..9829
                     /locus_tag="IV142_001550"
     CDS             9185..9829
                     /locus_tag="IV142_001550"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP8485215.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            9844..10173
                     /locus_tag="IV142_001551"
     CDS             9844..10173
                     /locus_tag="IV142_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485216.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            10187..11125
                     /gene="holB"
                     /locus_tag="IV142_001552"
     CDS             10187..11125
                     /gene="holB"
                     /locus_tag="IV142_001552"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP8485217.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            11161..11985
                     /locus_tag="IV142_001553"
     CDS             11161..11985
                     /locus_tag="IV142_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP8485218.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            11978..12325
                     /locus_tag="IV142_001554"
     CDS             11978..12325
                     /locus_tag="IV142_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP8485219.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            12394..13266
                     /gene="rsmI"
                     /locus_tag="IV142_001555"
     CDS             12394..13266
                     /gene="rsmI"
                     /locus_tag="IV142_001555"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP8485220.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            13375..14496
                     /gene="dinB"
                     /locus_tag="IV142_001556"
     CDS             13375..14496
                     /gene="dinB"
                     /locus_tag="IV142_001556"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP8485221.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(14550..15152)
                     /gene="nrdG"
                     /locus_tag="IV142_001557"
     CDS             complement(14550..15152)
                     /gene="nrdG"
                     /locus_tag="IV142_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP8485222.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(15467..17656)
                     /gene="nrdD"
                     /locus_tag="IV142_001558"
     CDS             complement(15467..17656)
                     /gene="nrdD"
                     /locus_tag="IV142_001558"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP8485223.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            17982..18749
                     /locus_tag="IV142_001559"
     CDS             17982..18749
                     /locus_tag="IV142_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8485224.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            18739..20781
                     /locus_tag="IV142_001560"
     CDS             18739..20781
                     /locus_tag="IV142_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8485225.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT         6608 a         3790 c         4246 g         6188 t
ORIGIN      
        1 aaaatggaat aaatcaacag ttaaactatt cttatcgata aatgaaagct ttaaaaataa
       61 atagatgttt aattaagaat aatcataaac tatttttact caaaaaaccg ctcagattat
      121 tttcagatct gaacggtttt ttttatctat tcatcaagat caaatttcat ttttcaatga
      181 ttcaatcaat tcgccctctg cttcatgact atgttttcgg ccattaacaa gatagagtgc
      241 tgtacatatc aatggaatga ctccggcaat cacatacaat cctctaaatg aagtaagaga
      301 ttttagcatc cccatcaaat aaggaccaac gcctaagcct aggtctaaac caataaagta
      361 ggtagataaa gcaatactga ttcgatgttc gcggacgatt tttaaacaaa cagcctgtcc
      421 atttgacata aacgtaccat aacctaggcc gactaatgct ccagagataa gcaatgtcac
      481 actattcgtc gttacactca gtaaaaataa tcctgctgtt aagaataaat agctaggata
      541 catcacgtat tgttctcctt tgacatcgaa aatccttcca gtcaatggac gtgtaaatgt
      601 gatgaccaaa gcatatacga cgaagaaaaa tgacccagca gcaactaagt ggattacttt
      661 tgtataagaa gataaaaatg ataaaacact tgaataagaa agacccatca aaaaggcaat
      721 tcctgtaatg aaaagtgctt tcttttcaat aaaactattg atattccatg agcgaagaga
      781 tattttttgt tcttcagata atggaatatt ttttacagga aagacaaggc aggcaatcgt
      841 aacaaagaag accagtacaa ttgaaaacca gataatgaaa cgaaaatcag tcatattcag
      901 caaaatcatt cctaaaaatg gcccaacagc ggcagctaaa ctagtactta atccataata
      961 attgatgcct tccccttttt tagatgcagg gatatatgca gtaacgatag catttgtagc
     1021 tgtagaaact gtaccgtagc cgaatccatt caagaatcga actaaataca atgccccgat
     1081 agttggcgta taaagatatg ctaatgttga aaggagatag aagatcgcgc cgcctcttaa
     1141 ggtaaacttc cgaccaaata gttctaactg tttccccatg aataagcgtg ccagcaatgt
     1201 tccgatgata tagatcccag aagcaaaacc tgcttggctc aagctggcgt gtaaggtgtc
     1261 ttgcgcaatg actgccatga tgaccatcaa cagataataa atcaggtaga caataaaatt
     1321 gatcaatgtg attccaataa aatatttatt aaataatttc ttttccatgt atgttataca
     1381 ttccccttaa gctgaatatg cttttaattt ttgtatttga acttctacat cgtgataatt
     1441 ggatggatag cgatcaacaa gttggcgaat aaataaatct cttgtagaca taaacagatt
     1501 taaaaattct tcgcattcgt ttgttggatt ctcatccact gctttgatca attcgatcga
     1561 gttttgatac gcatcattta aaaagactaa agaagctttc atatcaaatc ttgtttgata
     1621 tgctgtttct aatcgattaa tgattgtgtt catccagtaa ttcatgtttt ttccctctat
     1681 tcttaaaaaa ttttacttct gcagtataac gaataaaaaa aaggaaaaaa atcgcataag
     1741 tgatggaaaa aggtacttaa caaatgttaa tatcattagt aaaatacctt tggactttaa
     1801 aaagttttaa agtactgtat gatcttttta gatgaaagtt tggatggggg agcatcattg
     1861 gatagtagag tcttaaagga atatttggat aaaaatgatt ttccgattat acgcaaagga
     1921 tatcataaat atttgacatt cgaagggatg gaagattccc atacgtatat cttgaaagag
     1981 ggaacggtaa aagcaagtgt tatttcaaat gatgggagag aatttaacct tcgctatatc
     2041 aaagatcttg aaatcgtctc tcttttaaaa gacgaatatt cacaattcat cgattcgccg
     2101 tataatattc gagttgaatc agaatatgca gagttttatc gaatcaatcg tgtgcgcttt
     2161 tggcaaaata tcaaccaaga cccaaagcta caacactatg taaaagagta ttaccgctat
     2221 cgtctgcttt attcgatgaa aaaaatgcag caaatgctat taaacggaaa gctgggagct
     2281 gtttgtacgc aactttacga attatatgat ttattcggtg tgcggacaga agaagggatg
     2341 ctgatcgatt tttcgatcac gaatgaagaa atcgccaaat tttgtggcat ctcaactgct
     2401 agcagtgtaa acagaatttt aaaacaactg aaaaaagatg gtattatcac cgttaaagac
     2461 catcatatcg ttattttgaa tatggacttg ttagaagaca atattatctt ttgatgaaca
     2521 aagaggttgt gaaatcagcg ttttgacctg agtattagag aaacaaacgg aaaaatagtt
     2581 cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa caccgtttac
     2641 caagaggttg tgagcctgcc gtttaactct gagtcacaag gaacaatttc aaaaaacagc
     2701 ttttcatgtt tgatgaatat tgtgacttgt gaccgaggag ttggtaggag aacaccgttt
     2761 accaagaggt tgtgaaaaag ctgttctgca tcaagtaata agaagagcca agtaaaaaca
     2821 gtctcttatg tttttaactt ggctggactt atcaccgtag atgtaagctt ttgaacgccg
     2881 tttatttgca acaaaagggg ctgtgacacg atttttgtca cagccctttt tttgcgaaca
     2941 ttcatttagc aatttttaga aacttctgcc gccaccgcca aaagtcccgc caccgctaga
     3001 atgggtagta cttccgccac cgccgcttcc aggaggtggg cttttgggga ttcttcttgt
     3061 cgtgacaaaa gaatttgtca gacgatcggt tttttcagtt aatttgatac tggatttttc
     3121 tcggaatgga tatttgtaga tgcctgattt caattgataa cgcgaaacag tcacaaaata
     3181 aaagacaaga ctaaggataa gagccaaaac aacagcaatg atgatttcta ctgtcgtcaa
     3241 aaccttatag cgtgtaattt tgccagtttc ttcgtctaca cgataatgtc ctcctggaac
     3301 tccgtcttcc acatatgttg ctgatttaga caggtaagct tttgcagcat tgtaataggc
     3361 agcatttgtc atctgatcat aaacatcatc taagatcgaa tcgattctgc tatcagtcaa
     3421 ataatcgatc atattaccag aagtcgaaat gtagatttca cgctgtccca tatctaatag
     3481 caatactgag ccattttgat catttccgac tgcgtctctt aagtaattat cagcaaagtc
     3541 tcttggttct tctgaattag atgtagtcgt tacgatcaag actcgtcctt tgattttttc
     3601 attcagcttt tctgcctgtt gttccaaaga ttggatttga tctgttgtga ataacccagc
     3661 ctcatcattt accgtagctg tatctgcatg agtagccacc ggccagccaa cacttactag
     3721 caaaagaaag aataaaaaaa attttttcat catatcaagt aacctccgat cagaaagagg
     3781 atcgctagcc cagcgaaaat cccaagagcg gtcagtccca attttttctg gctgatcggc
     3841 aggacaccgc tgacctttcc tgtttgtcca ttcattgcgt agtagtagac ttttttactt
     3901 gagtcattgc ttcgataagt aacaagccat accggcagta atacgtaatt atttacttcg
     3961 tttgtgattg aagcagaagt tcgcacaccg gtcaatgttg tatatccatt tgcggtatct
     4021 tttaacagct tctctgaata gtcttttaat tcagattgaa tctgtgattt gattgcctca
     4081 tattcgatat ctcttttttc tgcttggaac ccggctaaat actggctttt aaaatcaatt
     4141 gctttatcta acgggaaagg ttgcacgcct tcgaccatct tttgttgcgt attttttgat
     4201 aaggcatttt ttactagttc tttaaaagaa agcttgcctg ctcttgaaac agcaaattgc
     4261 ttcgtttctg tgtattcgat gtcacctatc cgccatatgc ggataactgt accattcgcc
     4321 tgcatggcac catctacttc agcatctgta gcccaataag ggaaatacac acctgtcaac
     4381 ttatcgattt gatccttatt gaaaaaagct ttgggaacaa accatttttt cttggtccaa
     4441 gcgagaaatc tgttgattgc ttcttctttc tcgacagcaa aaggaagtac tttatttggc
     4501 aagaattctc cgcttaaacg tcccgatagt acgacgggat tatggcagta gtaacaataa
     4561 gtcgcagcag ttgtcgcatc agtcacgatc tctgcaccac agttgggaca aagaaaaaga
     4621 tctatcgctg cggtattatc tgcagattcg tccgctgtat ctgaaaaacc gcctgcctcg
     4681 tcaaccgctt tgcgctcggc ttcattcata tcggcaagct gttcttgggc tgtaaaagta
     4741 aaatcttcgg taggtgaagg ttcttctttg ctttcttcgc cagccatacg cgcttctttt
     4801 tgcttttgct catattggct gacttcttct tctgtataga tattcaggca gtattcgcaa
     4861 tgaaattttt ggtctttggg atcaaaagtc aacggaccgc cgcaattagg acatttatgt
     4921 gtaagaacat ccatcagctg cacacctttc taaataaatt aattaacggg gattccttta
     4981 aacggtttac cacagtttgg acagaattta gggatgccgt tggaaagatc cacgatctca
     5041 ttgcattcac tgcatcgcat ttgtagctta ggctgaacgt tttctacagg tttggcagct
     5101 ccgcagttgg aacagaattt tccggaattt tctgtcccgc aaactggaca tgtccaagta
     5161 tccgcatttc cttgtgcagt ttgttgattt tgtttttctg cttgttgttt gatttgttct
     5221 tggttatttt gagctgcttg agaaagataa gaaccatttg cattcattcc catacctaca
     5281 cccataaagc cggtcattgc tcctgcttca ttttttccgg ctgcttccat accacgtgca
     5341 atggaacctt gcacgtatcc ttcacgtacg ctaggatcac ctagcatcgc cccttcgtta
     5401 cgcatattga tcagtttcac agaatcatcg gtgtaagaaa tgctggctac agccacagaa
     5461 acaatctcca tcccacgtaa ttcacgccaa gaatcatcca atgcagtatc catgtatttg
     5521 cttaattcca aactcttaga tggtacataa gaaatccgtt gaccatctgc agacatctga
     5581 ttgattgctg attgtagcgc tgttaggaat tcagctaaat actgctcatt gatatcattg
     5641 atttccactt gtgttttgtt tttagggatt gcgtttgtat aaaataaaat cgggtcaacg
     5701 acatgaatag aataagtgcc atgtgcccgt aagaataatt ctgcgttata gaaattgtca
     5761 aagtagttca acggcgcgct tgtaccgaat ttgattccct ttatttcttg gagattgata
     5821 taaaaaactt gttgtttctg cggtgtgacg ccgccaaatt tgaatcggtc aaaagtttca
     5881 gcaattgctt ctttcagtgt tccattgaac atggaaggag ctgcgtcatt tttaatggtg
     5941 taataacctt cctcagcggt ataatcgatg atttttccac catcgaccaa aagcatcatc
     6001 atgtttggat agacatggac gactgttccg tctgttaaaa catcttctgt tcctttacga
     6061 ttggagccac gtttgtcatc tttgcggact ttgacacctt ttgtcatgac cgttgtatca
     6121 ctcatattgt ctggttcgat gacttctagc cattgatctg ccagaccgcc gccaaccata
     6181 cttgttgctg ctttgataag acccataaaa aattcctcct ctgattcagc tagtgccaaa
     6241 caattattgt ttatttatta taccatagga agatgtgaaa cttctcggac tagagacgga
     6301 attgaaatgg aaaattgcgt attgtttaaa tacctgaaaa agccgagaag gatagtagtt
     6361 tttttaccta atatcacgta taataagaga tagtgaattt tttaaaaaag ggggatcctt
     6421 atggcatatc aagcacttta tcgagtctgg cgttcgcagc gtttcgaaga catcgttgga
     6481 caaaaagctg tgacacagac actaaaaaat gctattgttt cccacaaaac ctcacatgcc
     6541 tacttgttta caggacccag aggaaccgga aagacgagtg cagcaaagat tttcgcaaaa
     6601 gcaatcaact gtccgaacag taaagacggt gagccatgca acgaatgcga aatgtgtcga
     6661 tcaattactg ctggtacgca agaagatgtg atcgaaatcg atgctgccag caataacgga
     6721 gtcgaagaaa tccgatttat ccgtgatcgg gcaaattatg cgcctacaaa ggcagcgtac
     6781 aaagtgtaca ttattgatga ggtacatatg ctttcaacag gagctttcaa tgccttgttg
     6841 aaaacattgg aagagccaaa agaaagtgtc atattcatct tagcaacgac cgaacctcac
     6901 aaaataccag ctacgatcat ttcacggact cagcgatttg attttaaacg gatcaatacg
     6961 gcagatattg tcgaacatct tgaatttatc ttgaaagaat cgactatcag ctatgaacca
     7021 gctgcattga atgtgattgc ccgttctgca gaaggcggga tgcgggatgc attgagtatt
     7081 cttgatcagg caatctcgtt tagtgaagga acaattactt tagatgatgc catgcaagtg
     7141 acaggtagct taactgacga aatgatggac agttacttga gtgcttgcgt gaaaaaagaa
     7201 gtaccagaag cattggaaac attgaaccaa attctcgcag ccggcaaaga atcacgtcgc
     7261 tttctagagg atatgttaca atactgccgt gacttgctga tgtatcagca agcacctaac
     7321 ctagttgagg aacaatcgag tacgatcaga agtacattta cagaattggc acaaactgtt
     7381 tcagcggatc agctgttttc aatgataaag attttgagcg atactcaaaa tgaaattcgt
     7441 tttaccaata gtgccgctat ttatttggaa gtcgctacag tcaaacttgc acagctaccg
     7501 tctgcttctg ttccttcagc agatgtagca tttgcagatg atcagatagt aaaacagcta
     7561 caacaagaga ttaaggaact aagaaacgaa atcaaggaac tacgcagtaa tacaggaatt
     7621 gagaatacaa aagtacagcc ggtacagcaa acaaatcata aaaaacagac aagtacctat
     7681 cgtgtaccga cggaacgagt atataaagtt ctgcgggaag cgacaagaca gcatctgaac
     7741 caagtaaaag aagtatggga agacttgctg atgagtctat ctgtcactca gcgagcaatg
     7801 cttaaagcaa gcgaaccagt tgcagctgga cctacaggac tagtgattgc gtttgattat
     7861 gagattgttt gtcaaagagc agcaaatgac gaagaattgc agctttcggt acacaacaac
     7921 ttaagccgaa tgatcaaaga ttatgcgcct gatcctgttt atattacaag agaaagctgg
     7981 ccagaacttc gaagaaatta tttgacgcag gcacaagaag agggaacaga aaactctttt
     8041 gcttctgaag actcagaagg agaaatcgag ttgcttcctc aagaacaaga agtaccagaa
     8101 gaaacacttg tagtaaaagc agaggaactg tttggctccg aagctgttac agtggtagat
     8161 gattaataaa aaggagtaga taattatgat gcgtggaatg ggcaatatgc aaggcatgat
     8221 gaaacaagta caaaaaatgc aaaaagaaat ggcagaagca caagagctgc taaataaaac
     8281 agagtttaca ggtaaagcca ccaatgaatt ggtcaaagcg gtttttacag gcgatcgtcg
     8341 catgaaagat atccaaatca atgaagaagt cgttgatcct gatgatatcg agatgcttca
     8401 ggatcttgtc gtaatggcag tcaatgatgc gatcgcacaa attgatgtgg aaactgagaa
     8461 aaaattaggg aaatacgcaa aaggtttgcc aggattttag ttttaaactg aaggagtaat
     8521 gaatatgcaa tatccagaac caattgcgaa actgatcgac agctacatga aacttccagg
     8581 aatcggtcag aaaacagcga caagactcgc cttttatacg atcgatatga aagatgaagt
     8641 agtaaatgaa tttgctaaat ctttgttaag tgtaaaacgc gatttgcatt tttgtagtat
     8701 ctgcggaaac atcacagaag aagatccctg cgaaatatgt agaaatcctt cacgtgatcg
     8761 aagcgttatc ctagtggtcg aagagccgaa agatgtaatg gcaatggaaa aaatgcgaga
     8821 atatcacgga ctttatcatg ttctccatgg tgtgttatca ccgatggaag ggacaggtcc
     8881 agaagacatc aatatttctt ctctgttgca gcggctgcat gatgcagatg taaaagaagt
     8941 gattatcgca acaaatgcaa caacagaggg agaagcaaca gctatgtatc tctcacgtct
     9001 gatcaaaccc gcaggtatca aagtgactcg tctagcacat ggtctgtctg taggctctga
     9061 tatcgagtat gcagatgaag ttactttatt gaaagctgtc gaaggaagac gtgagttata
     9121 aatttctaat taagtgtttt ttcagataca atataaaagc agcacaagag atgggggcaa
     9181 caatgtgaac ggactattta ttacaatcga ggggcctgat ggtgcaggaa aaaccagcat
     9241 cttgaatgaa ctttaccctc ttttgaaaga agtagcaaaa acagaaatca ttcaaacaag
     9301 agaaccagga ggcattccga ttgctgaaga gatccgagca gtgatccttg atccaaaaaa
     9361 tgatcggatg gatgaacgta cggaagcact gctttatgca gcagcaagaa gacaacatct
     9421 agtcgaaaag gttctgcctg ctctggcaaa agggaaaatc gttttatgcg atagatttgt
     9481 cgacagttct cttgcttatc aaggtgcagg ccgacgcatc ggcgttaccg agattgctcg
     9541 gttgaatgag tttgctacag aaggtactac gccggatttt accctttatc ttgacgtaga
     9601 ttcagacacc ggattaagaa gaatcaagaa aaacagacaa aatcaaatcg accgtttaga
     9661 ttcggaaggt ttggaattcc accagcgtgt acgtcatgct tacttaaagc tggcagaaga
     9721 gaatccggaa agaatccaca aagttgatgc aagaaaaagc tttgaagaag tgctgcaaac
     9781 aagttatcat acaattattg aacagtatcc gcaatttttt gaaaactagg aaggtgaacg
     9841 tttatgaaaa ttattttagc gattatccaa gacaaagaca gcaaccgttt atcaaatgaa
     9901 ttgattgatg caaatatccg tgccacaaaa ctatcatcga caggcggctt ccttaaggct
     9961 ggaaacagta cgtttatcgt gggtattgaa gatgaccgtg tagaagaagc attggaaatc
    10021 atcaaaaaaa cgtgtgaatc aagaaagcaa tttgtatcta cacctgttac actggatatt
    10081 tcgatggatg ggggcgtccc ttatccagta gaagtagaag taggcggtgc cacagtcttc
    10141 gtcttacctg ttgaaggatt ccatcaattt taggaggagc tgagctatga atcaagaaat
    10201 aaacttggct caaatgcaac cgatcgtgta tcagcagctt caacggagtt ttgagcatgg
    10261 gcgtatcgct catgcttatc tttttgaagg ggagaaagga acaggaaaac atgaagtagg
    10321 tatatggctt gcacagcacc tgttctgtac ccaaatgaaa gacaatctgc cttgtggaaa
    10381 atgcaacaat tgtcagcgga tcgaaaagaa agaacatcca gatgttttgg ttattgaacc
    10441 ggaaggacaa accatcaaag tcgatcagat cagacgtttg cagacggaat tcagtcgcag
    10501 cggttacgaa tcaagaaaaa aagttttttt gatcaaagaa gctgaaaaaa tgaatagcag
    10561 tgcggcaaac agtttgttga aattcttgga agagccccct ggagattttt tagcggtgtt
    10621 agaaacagat gcagtaggaa gaattctccc tactattcaa tctcgttgtc aagttctgca
    10681 ttttcaagaa ctttcgaaag aagcactgat ccatcgattg caagaagagc agattccatt
    10741 ggaaaaagca aaacttttgg ctttcttaac aaacagttta ggaaaagcag ttgaaatctc
    10801 tcaagatgaa tggtttaatg atgctaagga cttgatttat caatggttcg tttacttgca
    10861 aaagcaagat actcaagcct ttatctatgt ccaaaaaaaa ttggtaaaat catttaaaga
    10921 aaaaacacaa cagtttacag gattatctgt cttgatgttt tattatcagg aagcacagcg
    10981 tcaagcagta gcaaacggcg aatatggcaa agtgaaacag ataaaccaga taattgaacg
    11041 gatattgatc gcagagcaga aactacgcag caatgtcagt tttcaggctg ttgcggaaca
    11101 gttcgttttg catacgattt tttagcttgc cgcaaaggat gatgatcaag gaggatggaa
    11161 atggtagaag tagtaggtgt ccgatataga gatgcaggtc atatctatta ttttgcacca
    11221 ggagactctg aatatgttta tagtgacaag gtattagtcg aatctcagca atccttacat
    11281 attgctactg ttgtccttcc taaaaaagag attgatccca atgaccttcc tgaagctatc
    11341 cgtccgatct tgcataaagc gtcacaagct gaattagaaa aagacaaaaa aaacaaaaaa
    11401 gatgcttatg atgcattgaa aatcgcaaaa gaaaaagtgc agttgcgtga gttggaaatg
    11461 aaaatggtca aagccgaata cacatttgat cgaaataaat tgattttttc tttcacagcg
    11521 gacgggcgaa ttgactttag agaattggta aaagatctgg ccgctgtatt tcgtactcgt
    11581 attgagcttc gacaaattgg tgtccgcgat gaagcaaaga taataggcgg aattggtcca
    11641 tgtgggcgtc cattatgctg ttcttctttt ttaggcgatt tcgtgcctgt atcaatcaaa
    11701 atggcaaaag atcaaagttt atctttgaat ccaacaaaaa tttccggttt atgcggacgc
    11761 ttgatgtgct gtctgcaata tgagagcgaa gaatacgaga cagcgaaaaa agaactccct
    11821 gactttggaa aagtaattgc tacacctgat ggtaaaggga aagtcgtagg gttgaatctg
    11881 cttagccgta tcgttagtat tcggctgact ggacgggaaa atcctgtcga atatgaatgg
    11941 gatgaattga aagaatttat ccaagtaggt gagatgaatg gataagagat cattatacga
    12001 cggattgaat caattagaat cagagttgag aaatacatta acagaacttg ttgaaatgaa
    12061 gcatgctttg catgagatcg tagaaaaaaa tacaactttg gagatggaaa atcaacgtct
    12121 gcgggaacac ctgcgggaat tgaaccaagt tgcacaagct ccagctgaaa atgtgaagca
    12181 ggaactttct aagtctcgta tgaatcttga aaaaatttat gaagaaggat tccatgtctg
    12241 ctacgattta tatggatcaa gaagggacaa tgacgagcct tgtgcgtttt gtctagaagt
    12301 gatctatcgc gaaagcggtc gatgaccaat aacaagagga gactggagcg gagaaatact
    12361 gctccagtct ttttattaag gagattgtga cagatgtata atcaaaaaag tttcaaggga
    12421 aatccggtat atggggattt atatcttgta ccgacgccaa ttggaaattt agaagatatg
    12481 agctacagaa gtgttcgtat gctgcaagaa tccgatctta tcgcaagtga ggatacgcgt
    12541 aatacacaaa agttattgaa tcattttgaa atccatacac cccaaaaaag tttacatgaa
    12601 cataattata aagaacgtgt ccctcaattg attgctttgt taaaagaagg aaaaacgatt
    12661 gctcaagtca gtgacgcagg aatgccttcc atcagcgatc ctggacatga gctagtttta
    12721 gcctgcataa aagaagggat ttctgtgatc tctatcccag gacctacagc tgggatgacg
    12781 gctttgattg cttcagggct agtgcctcaa ccatttttat tttatggctt ccttccacgt
    12841 aagaaaaaag aacagaaaga aacattgaaa aagctaaaag aacagacagt tacgctgatt
    12901 ttttatgagt caccttatcg gatcactgct acattgacga atttcttaga aatttttgga
    12961 aatcgccaag tcgttttgtg tcgggaattg acaaaggtcc atgaagaata ccttagagga
    13021 agtacggaag agctactcgc ttatatcgaa gaacatccag taaagggtga atgttgtctg
    13081 cttgtagaag ggaatttgtt cgaaatgcca gtgacggaag ttgatgaaga gttgggaacg
    13141 ttaaaggaac aagtagaagc aaagatcgct tcaggcgaaa aaccaaatgc tgctatcaaa
    13201 gcagtagcgc tgaaaaatgg gctgaaaaaa caagaagtct accgtcagta ccatgaatta
    13261 gactgagata gtataagttg tctataatga aagactaatg aggaaatcag ctactaactt
    13321 ttcaaatctc ttgaacgcga acgaatgttc ttgtataatg aataagaggt gaggatgatg
    13381 gagctgcaat ttcctttaca taatgataca tcaagaaaaa tcattcatat tgatatggat
    13441 gcttttttcg cttcggttga agaacgagat catcctgagt ttcgcggaca tccattagta
    13501 attgccagac accccagtga cactggtggg cgtggagtag tgactacagc aaattatgaa
    13561 gcaagaaaat tcggaattca ttcagcaatg agtgcacaaa aagcgtatga gctttgtccg
    13621 caagccatct ttaagcctgg cgaccatcat aaatacagcg atatttccaa acaagtacgg
    13681 gaagtattct atcgttatac agatattgtg gaaccagtta gtatcgatga agcgtatctt
    13741 gatgtgaccg aaaataaaat caattgcaag tcagccatca aaatcgcccg aatgatccaa
    13801 gcagatattt gggagagtgt ccagctaaca tgttctgcgg gagtaagtta caataagttt
    13861 ttggccaaat tagcatcaga ctatcaaaaa cctagaggaa tcacggtagt tgcccctcaa
    13921 gacgctgtgc catttttaaa agctttgcca attgaaaaat ttcatggggt agggaagaaa
    13981 actgtaccta gaatgcatga gctagggatt tatacaggaa aagacctata tgaatgcaca
    14041 gaaatgatgt tgattcgaaa ttttggaaaa atgggctatt ctctttatcg taaagtcaga
    14101 ggaatccatg attctccagt aaacgttaca agagagcgaa aatctgtggg aaaagagcat
    14161 acttatgggc agccgttgca aacggaagaa gcagtgctga ctcagttgcg ccaactagct
    14221 gaaaaagtag aagaagcact gagaagagtc caaaaacatg ggaaaacagt agttttgaag
    14281 gtcaggtaca cggactattc tactgtgacc aaaagagtga cattgccaga atatatctat
    14341 aaaaaagaag cactctttta tcaagcaagc cttatatggg aggaaatctt gggagtagaa
    14401 aaaggaatcc gattgttagg gattactttg acgaatctgg acccgatgac ttatgaaaac
    14461 atcgtccttc cattatggga aaatcaagaa atataaaaac agctttgaga gttgaaaccg
    14521 gaaatccgtt ttctctccca aagctgtttt tatatcaagt cttgcttttt gatttgttca
    14581 taggtttctg ttgcatccgt acatttttcc caaatcacaa cttgattgga ttctaaagat
    14641 ttttggacgt caatgatccg ctggttgcta cttccgcgaa attgtagttt taggtcacgt
    14701 ttgcttaatt caaagcgtcc gtcaacaagg atatcgattt gcgaaaggag ttctaattta
    14761 tcttctgaat cttgtagcaa ttcatcaaag gtatagcctg tccatgacca gatatctttt
    14821 gtcgtaccga actcttttcg gatacgtcgt acgacttgca gacaaacatc tgtattcaaa
    14881 aaaggttcgc caccaagtaa agttaatcct tgcacgtaat cgttgcgcaa gtcagcgatg
    14941 atttgttctt ctaattcagg tgtgaatggt ttgccatatc ggaaattttg tgcagctttg
    15001 ttgaagcagc cttcgcaggc aaataagcag ccactaacgt aaagactgtt gcggacacct
    15061 tcaccatcga caaaattaaa cggtttgtag tcagcaatat agttttggct gtagtctttt
    15121 gctaaccatt cttttggttt tggatttctc ataaaaaaac ttctttcatt taaggttcaa
    15181 agatatagct attgaagata aaatctactc gaccacataa ataagaacaa actgacctaa
    15241 atccattgtt caagaaagct tgttcttatt ttgatagagg gcgttcaaag atacaaagcc
    15301 atcgaaagag ggtggtgaaa aaagacttgt catagcctct tattctgaat aaacggtgtt
    15361 caaaagcttg catctgcagt aataagccca actaaacgaa aaatatgagg aactattttt
    15421 gtttagctat tcttattact tgatgcagga cagctttttc acaacctcat ttcatgtgtt
    15481 tcacacgtga ggaaatttct ttatggcggc catgtaccat cggacgagct tgcggatttc
    15541 ctaaatagcc acaagtacgt ttgactacat cacatgtttt tggatcatga tttccacatt
    15601 ctggacattc gaaccctcgt tctgtcggtt tgaaatcacc ttcgaaacca cattgatagc
    15661 aatgatcgat tggcgtattc gtaccaagat acccgatttt gtcatatgca aaatcccata
    15721 cagcttctaa agctttcgga ttttgttgga gcatagggta ttcacagtag tggatgaatc
    15781 cacctgaaca atattttgga taatccttct caaattccaa tttttcaaat ggcgttgggt
    15841 ttttacgcac atcatagtgg aaactgtttg tataatattc tttatctgtg atattttcga
    15901 tgatgccgaa tttttctgta tccaaacggc agaaacggtc agttagactt tcacttggag
    15961 ttgaatagac actgaaatga taaccatatt catcccccca tgcatctgca ttagctttta
    16021 attctttgac gatatcaagt gtgaaagctt ttgcttctgg gtttgtttcc cattcgccac
    16081 cgaagaaagc tgaagcaact tcatacaatc caatgtagcc taacgataca gtagctcgac
    16141 ggtttttgaa taattcattg acagaatcgg tacgtgaaag acgtttcccg aaagcaccat
    16201 acatatataa aattggcgca tttgctggaa tggcttcttt acaacgttct acacgataga
    16261 gaagcgcatc tttcatgatt tctaagcggt cagacagtaa ctgccagaat ttattctgat
    16321 ctccttttga ttcgatcgcg attcttggca aattcaatgt aacaacacct aaattcattc
    16381 gtccgacatt tacttcttga ccattttcgt ctttccaccc ttgcaagaat gaacgacagc
    16441 ccataggtac tttgaaactt ccagttaatt caacgatttt atcgtaattc agaatatcag
    16501 gatacattct tttcgttgca cattccaatg ctagttgttt gatgtcgtaa ttaggatctt
    16561 cagggtttaa gttcacacct tttttcaaag tgaagatcag tttagggaag atagctgtcc
    16621 gcttttcact ccctaatcca ttgattcgaa tctgtaaaat tgctttttga atttctcttt
    16681 caaaccagtt ttctcctagt ccaaatccta atgaagtgaa aggagtttgt ccatttgaag
    16741 taaataaggt attgatttca tattccaaac tttgcatagc atcgaagata tcttttttgg
    16801 tttttgcttt tgcatattct ttttggcgtt ctggcgaatc gatccattct tccgcgtctt
    16861 ttagatgttt tttatagttc aattctgcaa aaggagcaag caattcatct gttcggtcgg
    16921 cagaacagcc accatattga cttgacgcta cattggcaat aatctgtgag atttgtgctg
    16981 tggcggtctg gatagatttt ggcgattcga cttcggcatt gccgattttg aacccattgt
    17041 tcagcatgcc tttgaaatca atcaagcagc aattcgtcat cggtgtataa ggatgataat
    17101 ccaaatcatg gtaatggata tcgccttttt gatgcgcatt tgctatatgt ggtggcaaca
    17161 ttttcagtcc gattgatttt ccaacgatcc cggctgtcag atctcgctgt gtattgaaaa
    17221 cattactgtc tttatttgcg ttttcgttca cgacagtttg gtccttgttg attagctttt
    17281 caatcgaaaa gttgatatct gttgctttac tgcgagcaaa atctcgtttg gtacgataat
    17341 tgatgtactc ttctgctaat tcatattcgt ttttttctag taatatatgt tccacgatgt
    17401 tttgaatctc atagattttg acattttcag caaaccggct tttgatttct tggacaacca
    17461 agtctgcaat catttgaatt ttttcataag taagtggtga taatgagccg tgaattttct
    17521 tttctgcttt gatcagtgct tcattgatct tttgttcatc gaaatcaaca tggcggccat
    17581 cgcgtttgat gactttcatc tgggatgtct ctggctgctg agtaacgaca gttttttctt
    17641 cataatttac tttcattatc ttcaactcct gctaaattgt tcttgtctat cataaaacaa
    17701 aatcggggaa ggagcaacta aatatcggct atatatagta ttcaatgtgc tgtataattg
    17761 gatttctata ctatatgtag tgtatatttt attgtgaaaa aaggtgaaaa cttgtagctg
    17821 gtggatttgt tttttgcata tttaattttg ctgaataaaa taattgaata aaatttttta
    17881 ttcagtccaa gtaaaagtga caaaaacata agagcaaatt attcgtagat atgctactat
    17941 gcaaataaat agaaatgaaa aatgaaagag gaggagaaga attggaaaag atgatcgaag
    18001 tgaaacatct aagtaaaagc tacggcagca aactttctcc cgtcaaagcc ttagataata
    18061 tctcattcac agtagaagaa ggcgagtttg ttgggattat gggcccaagt ggtgcaggaa
    18121 agacaacgct gatgaatatc ttgtccacga tcaatttgcc tacgatgggc agtgtctcga
    18181 tccaaggaaa agaaatcaca aaaatgaaaa acagtgagct cagtgacttt cgtcgaaaaa
    18241 aactaggatt catttttcaa gagttcaatt tgatcgatac cctaaatgca aaagacaata
    18301 tcctattgcc cctagcagtt gaacggatga ctaaagagga aatggaaaag agagtaagac
    18361 atgtagctca attgttgaat atagaagaac tgctcaaacg gtatcccgat gaactttctg
    18421 tcggacagag acaacgaatt gcagctgcta gagcacttgt tgttcagcca caagtgattt
    18481 ttgcagatga accaactggc tctttggact cgaagtcggc tacagaattg ttgaactact
    18541 tgaagaccat gaatcaaaaa gaaaaagcaa cgatactgtt agtcacccat gatccatata
    18601 cagcaagcta ctgcgataga attttattta ttaaagatgg tgtgattttt tctgaagttg
    18661 tcagaagagg gacaagaaga gagttctttg agaaagtaat agacatgcag gcgacgattg
    18721 gcggaggagg aaaaatgaat gctgtataaa ctagccgtta aaagtttttt tcggcagtca
    18781 cgaggctatc ttgtttattt tttcagtttg actttatccg taatgatcta ttattccttc
    18841 agcgcaatga cctacgatca atctttagtc agaagagcaa gtcaagatac ttcgatagat
    18901 ggtggattga gttttggtgg atttatcatt actgttgttt tgcttttttt cgtattcagt
    18961 gccaaccgat ttttcttgaa tagaaggcaa aaggaaatag gcatctatca gctttttggt
    19021 atgaataaac tgcagatatc aggtatatat gtgttagaaa tcatgataat tggattgttc
    19081 gcctgtattt ctgggatatt actcggtatt attttttcca aactcttttc aatgattttg
    19141 gttcgaatga tggatatgga tcttactagt ccatttttta tctccattcc atcagtagta
    19201 gatactttat ttgctttttt catcatcctt ttagcagtat ctgtatattc aatatggaaa
    19261 atatggcgct atccaatgat tcgaagtttt ggagaaaaag aacaagcaga tagttcaaca
    19321 atgcgcttca ggacaaggca ccgattactc gcagtcatcg ggttactact tttaacgagc
    19381 ggatattttg gagctagtca ttttagggaa atcacttcat ggatgatcag ttatcattat
    19441 cttgatacat tagttttctt ttttcctttt ttaatttttt tcgtttgtgt ggtcggtact
    19501 tatttgtttt tctgttatac atttcggtta tttttgatca tattgagtaa atggaagatt
    19561 tattactatg gtatcaattt tttgataatg ggtaacacgc aagttcattt gctcaaaagt
    19621 tggcgtacaa attctctgat tacagtcatt ctcggaattt ctttaatgac aattggcggc
    19681 acggctagta tctctactat tttatcctac caggagaaag tttcctcccc gatggactat
    19741 cagtttgatg ttgagacaga gaaaaaaatc aagccgattc tagcagagga agggcagaag
    19801 ataactaaag aaatcacctt gcattataaa gtcattggga gtatctatag tcaaaatctc
    19861 gaagaaccta tgtttcaaac tgctaatctg atcacggaaa cacaatatca agccttcaga
    19921 aaagttaatc cagatttgcc gaacatttct ttgaaatcac ctggtcatac tgttttgctt
    19981 gatcaaatgc aaactctttt tcggaatatt tcattatatg gatctagtat tggactgcca
    20041 gataatcaag aattgaagat tcaacaaatg cttccaagtg tattaggtga cgaaacaatg
    20101 gtatatctaa gtcctgtact gattgtagat gataccgttt ttgaaaaagc aaaaggtgtg
    20161 gattatcaag tagtcaatgt agatgtatca ggtggaaata atgaaaaaat agatgaacga
    20221 ctgagcaatg acctgtcgat aaagtggctc aatgctgttt attattctta tgatatcgta
    20281 aatggaagtc tacaaggaga aattagcaca aagaaacttc cggatgacca aatgaaggaa
    20341 gcacaattta gccaagaaag ctctcgtttg aatttctctt ccagctattc gaaattgcgt
    20401 gcagttaatc ggcgaatcgg tatcaatatt tttgtcacac tatttgtcgg gatgatcgtg
    20461 attattgcta ctggtagtat tttgacagtc cggcaattag cagaagcaga ggaagaaaaa
    20521 gaaaattatc acctattgac taagctgggg attccacaaa gaaggatcag acggatgatc
    20581 ttcgagcaaa acgcacttac cttctttcca cccatgatat tagggatcac tcacgcagta
    20641 tttgcaatca atgtcttcac tcagtatata aaaactgccg attactggtt ggcttatctt
    20701 gtgagtggct tattgatcct tatctatctt ttgctttatg tcattacgag tcagttatat
    20761 tgtcggatta tcgaggaatg atatacggaa taagacaaga taataaatag aactaaattt
    20821 tgaataccat tt
//
LOCUS       DACDEG010000042        19811 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.044, whole genome shotgun sequence.
ACCESSION   DACDEG010000042 DACDEG010000000
VERSION     DACDEG010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.044"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            251..526
                     /locus_tag="IV142_001561"
     CDS             251..526
                     /locus_tag="IV142_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485226.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            539..2611
                     /locus_tag="IV142_001562"
     CDS             539..2611
                     /locus_tag="IV142_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP8485227.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2776..4662
                     /locus_tag="IV142_001563"
     CDS             2776..4662
                     /locus_tag="IV142_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8485228.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4674..5621
                     /locus_tag="IV142_001564"
     CDS             4674..5621
                     /locus_tag="IV142_001564"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP8485229.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5641..6168
                     /locus_tag="IV142_001565"
     CDS             5641..6168
                     /locus_tag="IV142_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8485230.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6231..6884)
                     /locus_tag="IV142_001566"
     CDS             complement(6231..6884)
                     /locus_tag="IV142_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP8485231.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7016..7858
                     /locus_tag="IV142_001567"
     CDS             7016..7858
                     /locus_tag="IV142_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8485232.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8016..8912
                     /locus_tag="IV142_001568"
     CDS             8016..8912
                     /locus_tag="IV142_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8485233.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8915..9628
                     /locus_tag="IV142_001569"
     CDS             8915..9628
                     /locus_tag="IV142_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP8485234.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9648..10166
                     /gene="msrA"
                     /locus_tag="IV142_001570"
     CDS             9648..10166
                     /gene="msrA"
                     /locus_tag="IV142_001570"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8485235.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10163..10390
                     /locus_tag="IV142_001571"
     CDS             10163..10390
                     /locus_tag="IV142_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP8485236.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10542..11093
                     /gene="lepB"
                     /locus_tag="IV142_001572"
     CDS             10542..11093
                     /gene="lepB"
                     /locus_tag="IV142_001572"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8485237.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11176..11718)
                     /locus_tag="IV142_001573"
     CDS             complement(11176..11718)
                     /locus_tag="IV142_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP8485238.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11955..12353)
                     /locus_tag="IV142_001574"
     CDS             complement(11955..12353)
                     /locus_tag="IV142_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8485239.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12613..13473
                     /gene="ylqF"
                     /locus_tag="IV142_001575"
     CDS             12613..13473
                     /gene="ylqF"
                     /locus_tag="IV142_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP8485240.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13466..14233
                     /locus_tag="IV142_001576"
     CDS             13466..14233
                     /locus_tag="IV142_001576"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP8485241.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14289..15146
                     /gene="dprA"
                     /locus_tag="IV142_001577"
     CDS             14289..15146
                     /gene="dprA"
                     /locus_tag="IV142_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP8485242.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15268..17346
                     /gene="topA"
                     /locus_tag="IV142_001578"
     CDS             15268..17346
                     /gene="topA"
                     /locus_tag="IV142_001578"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP8485243.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17315..18691
                     /gene="trmFO"
                     /locus_tag="IV142_001579"
     CDS             17315..18691
                     /gene="trmFO"
                     /locus_tag="IV142_001579"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP8485244.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18889..19794
                     /gene="xerC"
                     /locus_tag="IV142_001580"
     CDS             18889..19794
                     /gene="xerC"
                     /locus_tag="IV142_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP8485245.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT         6873 a         3414 c         4120 g         5404 t
ORIGIN      
        1 tcgcagcccc atttttttgt tgtgataaaa gaacaagaaa ggcaggaatg ggaaagtaaa
       61 catccttttg tcagaaactt ttatctttct taagttttac ttgatatctg atactatttg
      121 gaatagaatg aggcgttgaa aaatttatgg aaatttctaa ctagcaataa acgatgaaac
      181 aatgaaatat ttttcatcca aaaagaactt tgctataatg atggaagttt gtgtatagga
      241 gatctggaaa atgagagaga gttttgaaca acaaaaaaaa ctgttgcatg atcgctatgg
      301 cgccctttcg atggatgacc gccgtcaaat actttgtaag ttgcgtaaaa gaaatatatt
      361 gatgtatcgt cagttggaac gtctgaaaca tgatctgctt cgtttggagt caaaacgtgt
      421 acagtgtgaa ttagagggga accaaacaca ggttgaagta gtcgaaacca aaattttaaa
      481 gaaaaaagaa caatttttaa aaatgcttac acaaaataaa aaataaaggg gatggacgat
      541 ggaatttgct gagttagtta ttgtatttgc agtaacgatc accttatcga acatagctag
      601 ccgcattttg ccaatgattc cagctccact gatccaaatt ttccttggag tggtattagg
      661 tctgacagag tggggacaat caatcgattt tgaacctgaa ttgttcttag ttatgattat
      721 tgcaccattg ttatttagag aaggagaaaa agcagatatt tcttcgattc tcaaaaattt
      781 cgggacaatt ttgttcttgg catttggagg agtcattttg acccttgtcg gggtaggtgc
      841 gacgttatca ttcctgttgc caagtgtccc acttgctgcc tgctttgctt ttggagcagc
      901 gttaggtccg acagatgcgg tagcagtcag ttctctttct ggacgagtga atatccctaa
      961 aaaggcgatg catattttgg aaggagaagg tcttttgaat gatgcatctg gtgttactgc
     1021 cttccagttt gctcttggtg cgctgatcac cggatctttt tcagcagtaa acgcggggat
     1081 gagtctagta gtttctagta ttgggggagc tttgatcggc tttttgcttg tctggttcaa
     1141 acaaaaaata attcatttga tcgaaaaagc atcagcacaa gatgtcacag ggtatctgct
     1201 gatcgaactt ttgcttcctt ttctagccta tgtattggca gaggcatttg atgtatctgg
     1261 aatcattgct gcagtggcag caggtattct ccaagcttct ggttttagga aaatctctgt
     1321 gtttgatgca gaattgtcga gcttgtccca cagtacatgg acaactattg cttttacact
     1381 gaatgcatta gtatttatat ttttaggaat cgaattgacg caagtgttct cgccagtatg
     1441 gggagacggt ttatatccaa acggtctttt attagcgatt atcgtattaa tctctgtcat
     1501 gctttttgtg atcagattca tttctatcag tcttttttat gtattcaaag atggcagtaa
     1561 aaagttcaaa aaacagttga acgagatctt gatcttaaca tttggcggtg taaagggaac
     1621 agtcagtttg gctacgattt ttattttgcc attttcaatc aataatatga tgttttacca
     1681 gcggtctttg cttttgtttc tgacggcggg tgtcatctta gtgactttgg tcatagggat
     1741 cattgtcttg cctatgctga cggaaacaga ggaagcgaaa agcacggatt tgaatgcttt
     1801 gatgatttta gaagaggtcg ttgaagtttt gcgaaaagaa ataaaggaaa ttgatcgtaa
     1861 tacaaaagaa tttttagcca ctgaagcagt gatagaaaat tatcaagaac ggatacgtga
     1921 tctgtacttg gaagatttaa ccgatgatga aaagcaagaa gtacaagaaa tacaagcact
     1981 gattttgtcg atcgaacggg atggattgga tgaaagttat cgttcgggca agctttcttc
     2041 taatggttat cgtttttact cccgttttct ttctcgattc gaacattcta tcacaagcca
     2101 gattttgtct tttatcggct tttggttcat tgttgtccgt aggttaatgc gaatcgttct
     2161 ccatccgaaa atgttttggc aaagaagata tgctgatcgt cagacattta ttagtgatga
     2221 agatatccaa gaaatcagag agacatatca aaaaaatact cagttgatta ttgaaagttt
     2281 agataacttg actgatattt atgatgaaac gatggttcaa ttttttatcc agcaacgcaa
     2341 acttgaagga ataaaaatga actcggggaa tttgatttct tcgtggatga tccagcaaga
     2401 tagtctgttt acgaaaaaaa tgctaagagg ttattatctt gaaagaaaaa tcattgatga
     2461 atacgaagtt gctgaaaaaa tcacgacgtt ttctgcgaac agctatcgca ggaatatcaa
     2521 tttgttagaa tcttatacga tgaacaaacc atctgataac ttttctttcc gatttgcgtt
     2581 tcgagcaaag tcaaaaaaac gagtccaata aaataaaaag gtaaaaattt tcaagtttta
     2641 taaaaaacaa aacatatcag aacaggatcg aatcggtcct gttctttttt tgagaacaca
     2701 aaaagaacaa ataccaaaaa aattttcctg aagttcacta tttttatgat acaatatcca
     2761 agataaggag ggaacatgtt gaaagaatta aaagtaactg acctcaaaaa aacttatggt
     2821 gaaaaagatt tgtttgacca gatttctttt ttggtccacg aaaaagaccg tatcgggttg
     2881 attgggacga acgggacggg taaaacaagt ctcttaaata ttattgccgg tatcgatagc
     2941 agcgacggtg atcggcaaac agtgttttat ccaactgatt accgtattgg ttatctttct
     3001 caagcagctg aattttccga tgaattaact gttctccaag cagtctttca aggaaacagc
     3061 cccttgatcc aaactgttcg agcatatgag gaagcattga tcgaactagg ggaaaacgga
     3121 gaagaccctg atgtgcaaaa acgttatgct aaagcagaag aacaaatgaa taaagaagat
     3181 gcttggacta cagataccaa cgcaaagatt attttgcaaa aattaggaat cagtgaatta
     3241 gataaaaaaa tcagtacttt atcaggtggt cagaaaaaac gagttagtct ggcgcaagtg
     3301 ttgattgatg aacccgattt actattacta gacgaaccaa caaaccattt agattatgaa
     3361 gcgatcgagt ggttggaaaa ctatttgaaa caataccgtg gcgcactatt gatggttacc
     3421 cacgatcgtt attttctaga tagagtagca aaccgaatct tcgaactttc atttggaaaa
     3481 ttatacgagt ataaaggaaa ttatgaagct tacgtcttag aaaaagcaga acgtgatcgt
     3541 gtagaagtag aacaagaaga aaagcgcaag cggctttata aacaggaatt agcttggatg
     3601 agggctggcg tacaggcacg tggcaccaag cagcaagcac ggatcaaccg tttcgaagat
     3661 ctaaaggaga acttgtacca agtaaaccaa gaagatgatt tagagctgaa cctagcgaca
     3721 caacgtttag ggaaaaaagt actggaaatc aaagatggca gttaccgtat aaatgaccaa
     3781 acactgctag aacatttaga tttattgatt caatctcggg aaagactagg tattactggt
     3841 aaaaatggtg cgggaaaatc gacattgctt aatattctag ctggcagaat tcccctggat
     3901 agcggtacaa tgtctatcgg tgaaacggtt catcttgcgt attatacaca agaaaatgaa
     3961 gaaatggcac cagataaacg gatgattgct tacttacaag aagctgctga agaagcaaaa
     4021 acagcagatg gcagtcaaat cggagtagca gaattacttg aacgattttt atttccaaga
     4081 tttatgcatg ggacactgat aggcaaactt tcaggcggag aaaaacgccg gttatttttg
     4141 ttgaaactgc tgattcagca acctaatgtt ttattattgg atgaaccgac caatgatttg
     4201 gacatagcaa cattgacgat tttggaagat tattttagaa gttttccagg tgctgtgatc
     4261 actgtttccc atgaccgtta tttcttggac aaggtagcag ataagttatt agtattccaa
     4321 ggaaacggaa aacaagaact ttattacgga aacatgtcca gttatttact gaagcaaaaa
     4381 gagacccagc agcctgctga aaaagcgaaa ccaaaaatac agagcaagga acctgctgaa
     4441 aagaagaaat tatcttacat ggaacaaaaa gaatgggaaa cgattgaaga tgagattgca
     4501 gaacttgaag aaaaaatctc attgctccaa gaagagatga atcatcaagg agataatttc
     4561 actcgattac aagaattgca aaacgacgta tctgaaacag aagcacaact tgaagaaaaa
     4621 atggcacggt gggaatactt gagtgaatgg gtagaagatt aggaggaaaa aggatggaac
     4681 aagcgtatct tgacttagga agaaaattgt tggaagaagg gcatcgtaaa gaagacagaa
     4741 caggcacagg cacattaagc ttattcggat accaaatgcg gtttgatctg caaaaagggt
     4801 ttcctttgtt aacaacgaaa cgagtacctt ttggattgat caaaagcgaa ttgctttggt
     4861 ttttaaaagg agatacgaat atccgctatc tattacaaaa taaaaaccat atttgggatg
     4921 aatgggcatt tgaacgctac atcaaaagtc cagactataa tggaccagat atgactgatt
     4981 ttggccatag atgtttgaca gatcctgaat tcaatgaaga atataaaaag cagagcaagc
     5041 ttttttgcga gaagatccta accgatgatt cctttgctga aacatacggc gatcttggac
     5101 acatctacgg ctatcaatgg cgtcactggg aaacaaaaga cggtggcttt atcgatcaaa
     5161 tcaaacaagt gatcgaagca atcaaaaaga ctcctgattc tagaagattg atcgtttctg
     5221 catggaatcc ggaagatgtt cctagcatgg ctttgcctcc ttgtcatacg atgtttcaat
     5281 tttatgttca agagggacgg ctaagctgcc aattgtatca acgcagtggg gatgtgtttc
     5341 taggcgttcc gttcaacatt gcaagttatg cgttgctgac tcacctaatt gcacatgaaa
     5401 caggtttagc ggttggcgaa tttgttcaca cattaggaga tgctcatctt tatatcaatc
     5461 atatcgaaca aatgaaagaa caattgagcc gagaaatacg cgcatttccg actttagttt
     5521 tgaatcagga aaagcagtca gtatttgatt ttgagatgga agatatccaa ttagaaggat
     5581 atgacccaca tccggcgatc aaagctccta tcgctgttta gaatcagaaa ggatgagaaa
     5641 atgttagctg caatttgggc acaggatgaa aacggattga ttggaaaaga agaccgtctt
     5701 ccttggcgtt tgccaaatga tctgaaattc ttcaaacaaa tgacagaagc aaatacacta
     5761 gtgatgggac gaaaaacatt cgaagggatg ggtagccgtc cgttgccgaa tcgtcagacg
     5821 attgttttga cgagagattc ttcctataaa gcagaaggcg tgcatgtcat gcatagtgtg
     5881 gaagacgtgc ttgctctgga aaaagaaaca gacggtatct ttttcatcgc tggagggtca
     5941 gctgtttatc aagaatttct gccattttgt acgatattat accgtacagt catccatcat
     6001 gtattcgatg gagatgctta ttttccacct gttgactggt ctgattggtc actgatcaat
     6061 actagtcaag gagaaatgga tgaaaaaaat cgttatccgc atcaatttga aacgtatcag
     6121 cgtaatgaga atcctgtaaa aagcgggaca ggcgaattga cgatataaaa aagctatgga
     6181 tagactagct gttatttaga gctgtcccat ccatagcttt atttttttta ttaagtaaag
     6241 aataaaacag agaagtacat aaaacctgca cctaacataa caaacaaatg ccaaacaaca
     6301 tgcatatagc ggatagaatt caagctataa aagaatgcgc ctatcgtgta agaaagcccg
     6361 cctgctacca tcaaaataac accagtcatt ccaagagagt gataaagagg aacagcagcc
     6421 acgacacaga gccaccccat aaaaatatag atgatcgtag atacttttga tactctctcc
     6481 cgtttatgca acgtcaacgc tttatatata atgccaagaa tcgcaaacaa ccagattaat
     6541 acaaataaag tccaacctag ccatcctcgg atactcagca aacaaaaagg agtataactt
     6601 cctgcaatca aaagaaaaat cgaactatga tcgaatactt ggaacacctt ttttgctttt
     6661 gtaaaaatca atgaatgaaa caaagtagac gttaaaaata aaagaatcat catcgatcca
     6721 tagatagcat aagagacaat atggagggag gaatgcaagt gggcaccttt tactaaaaga
     6781 accactagtc ccgcgatact caatccagtc ccaattccat gggtgactgc attaaacact
     6841 tcatttaata tttcatacgt tcgattaaat ttcgtttttt ccatgaaaac ggctccttta
     6901 ctaggtgtaa ttaatgaaaa atcattattt gtctgttata ctaaaaatag aaattgtctt
     6961 acatgtagtt taacatgaat aaggcatctt gttaaagaaa gagagagaga aaataatgac
     7021 aaatgtaaaa atcgtaacag attcttcatg tacgatggaa aaaaatgtac gcgatgcttt
     7081 aggcattcgt gtcatgccgc tatccatcat gatcgacgga acagtatatc ccgatgatga
     7141 ccaactagac ggcgaaagat ttatggaaat gatggcgaaa gcaaaaaatc tgcccaaaac
     7201 aagccaacca ccaattggag aattcatctc gatgtatgat gagctaggag aagacggcag
     7261 tgaagtgatt tcgatccata tgacaaaagg cttgagtgga acagtagaag cagcaagaca
     7321 agccagtaat ctaacaaaaa cgaaagtaac ggttatcgac agtgatacca ctgaccaagg
     7381 attatctttt caggtaattc gagcagctga attagcaaaa gaaggtaaaa cagtagagaa
     7441 aatccttgaa gaagtcaaaa aagtacaaga caatacaaaa ttatacatcg gaatatccac
     7501 actagataac ttggtaaaag gtggaagaat cagccgaaca actggtttgc tgtctagtgt
     7561 tttgaacata cgcgtggtga tgaatttcgc ccattctgaa ttaatccctg taactaaggg
     7621 gcgaggaaag aaaacgttcg ataaatggtt taataatttg aaaaatgaac tgaaagaatt
     7681 gccaaatgtg cgacaaatcg gtatttcaca tgcggatgca aaagaattgg cgcaagaatt
     7741 caaagagggt ttgcaagaat tattcccgga catgcatatc cctgtactgc atacgaatcc
     7801 ggttatcgct acgcatactg gtaaagacgc gtttgcaatc atgtattatg tagattagtc
     7861 gaaaagagga taagccctca gcctgaaagg cttgcagctt attctctttt tctttataaa
     7921 attaggaaaa taattgtttt cttttttcat aaagactacc tttttgataa aatttttgat
     7981 aacattagaa ccaatcgaat acaagaggtg aaaaaatgaa attaacgaaa cgacatttga
     8041 tcctcgtttt gttcccatta ttaattgcga tcatcatcta tgcaggttta gctgcttcta
     8101 taccgaaagc tggcccacta atgaaaccag taactactgt gagagaagat ccaaataaaa
     8161 aagaaaaact tcactacata gctatcggag attcgctgac tgaaggagta ggagatcaaa
     8221 ccaagcaagg aggattcgtt ccccttgtcg caaatgatct aaaagaccgt tataatttga
     8281 ccgcggttga aacagaaaac tatggtgtaa atggtgaacg aagtgaccag atcttaaagc
     8341 gagtaaaaac aaaagaagaa attcgaaaca atatcaaaac agctgatttt atcacactga
     8401 ctgttggcgg taatgatttg atgaaagtta tccaaaataa tttattcggg ttaacggtca
     8461 attcctttaa aaagccactg aaaaactatc aggaaaatgt cacagaacta ttggaagaat
     8521 ttcgatccct caataaaacc gcacctattt acattttagg catctacaat ccattttatt
     8581 tgaactttcc tgagatcact gacatgcaga caattgttaa caattggaac aaaggaacag
     8641 aaacggtgac aaaaaaaatg ccgaactgct actttatccc aatcaatgat ttgctttatc
     8701 aaggattgaa tcaggaagta ggtgtcacag aagaaggaac agatcaaaca actagcacga
     8761 cagaaaatct aaatggcatc aaaaataatg tgctctatga agaagatcat ttccatccaa
     8821 ataatttagg ttatcaattg atggcaaatg cagtgagaga tcagctgatc gagacacaaa
     8881 accaatggtt agagaaggag gatgatcaat aaacatgaaa acaagatctg acgtatcttc
     8941 aaacaaaaca aagaaaacaa attactggaa attcgccttc cttatccttc ttggggtagt
     9001 acttggttcg accgtatttt taggcagccg tatttttgct aatcgggaac cagaactccc
     9061 agaaatacca gctcttacgg aaagacaagg agatcctgtc ttaacgatca attcaaataa
     9121 agaaaaagtc aatcaaatca tcagcttttt ccttagtgag tatcaaaaag atagtgatat
     9181 tgaatataaa ttttatttgg aaaatgaagc actactcaat ggtactttcg aagtactagg
     9241 ttttcctatc gatttctatt tatattttga tccgtatgtg atggaaaacg gaaatgtcca
     9301 actaaaagct agaagtcttt ctatcggaac attgagtttg ccaatcaaag atgtgatgaa
     9361 tatgatcaag agaaactata aattaccaga ttggatcgaa gtcaatacag atgatctgac
     9421 tatcatgctt aggttggatc aattccgaat gcaaaacggg atgtatatta aagcagataa
     9481 aatcgactta gtaaatgatg acattcggtt tagtctatat ttgccaaaag atacctctga
     9541 tgcttcaaac aactcaaata ccaacaaaac tagcaccagc agtactgaaa gcaagacaaa
     9601 agaaaataaa aaatcatcgg atgagtagta ttagaggggg aatcataatg gaacgagcaa
     9661 tatttgcagg aggctgcttc tggtgtatga tccagccttt cgacacgctt cctggtatcc
     9721 atacagtgat gtcaggatat actggagggc atgtaccaga tccgacttat gaacaagtaa
     9781 aaagccaaac gactggacat acagaagcag tggagattat ctatgatcca acattgattt
     9841 catatgaaca attagtagaa ctttactggc agcaaactga tccaactgac gctttcggac
     9901 aattcgagga tcgcggtgac aattatcgcc cggtgatctt ttatttgaca gaagaacagc
     9961 ggatcattgc tgaaaaaagt aagaaacagt tagctgaaag tggacgtttc gaacagccaa
    10021 tcgttacaaa aatcgaacca gcccaaccat tttatctagc agaagaagac catcaagagt
    10081 attataaaaa gcatcctgaa aattttaaaa gaaaccatgc acgccgggct gcctttatcg
    10141 aagaaaattg gaggaacgaa gggtgaatcg aagctttttt cattatttaa tgacactgcg
    10201 aggcggtggc acacatgatg ctttatctgt tttcgctaca gaagccggta aagacatcca
    10261 atttcctaag cacagctcta gctacgaaga aatttctaat tatctggaac tgaatgtcga
    10321 ctatttgccg tccatggata tctttgacga agcatgggaa aaatatttag aaaataatca
    10381 tgacatataa gagatagttt tgtactttat gatcaaaaaa gttttttgtc caatagccta
    10441 attgaaagaa ttatttttat ttcagctatc tagtcgcatt tttttgatat ttttcttata
    10501 ataaagaaag aatgaaaaaa agagggatgg attgatagag attgacaaaa aaacaacgct
    10561 atattaatcg cttctggctt gtattcaaat acttacttgt ttctgtattc cttgcgttca
    10621 tgttgcgggg atttttattc atcccggtac cagtggaagg caattcaatg gaaaacgtgt
    10681 tgaagcaagg tgatatggtt gtgatggaaa aattttcgga gatcagacga tttgatattg
    10741 ttgtttttca attagcagat ggcacgattt atatcaaacg agtaatcggt cttccaggag
    10801 aaaatgtcag ctaccaaaat gatcaattga agatcaatgg gaaagtagtt aaggaacctt
    10861 atctaactaa aaacctgaaa tcagatcatg caaatgcatc ctatacgacg gactttacac
    10921 tacaagagct aacgggacaa tccaagcttc cggaagataa ttattttgta cttggagata
    10981 atcgaagagt ttcaaaagat agtcgttcgt ttggcacgat caataaaact gatattctag
    11041 gtaaagcaag atttgtctat tatccacttg atgaaataaa atggatccag tgagaaacaa
    11101 taaagcgtgc agtctgttga gactgcacgc tttattgttt aatccaaaat cagtttacta
    11161 ccttagtacg taaaatcaaa cagctcgttt aaacactttt ctagcctcta aagttttgat
    11221 aaatgaataa acaaccatta tttgaatagg gatttggatc atattcctga tgaatctacc
    11281 tagaaaaatt actttgaaat ctgcaccaaa caaaacagac atcagccaag aattgataaa
    11341 aaggctgact gggatcataa tcaataattg ggcagcgatg acggaaggca ggtttatctt
    11401 tttatgatat aaaaataagc cattaataaa tccaccaagg aaagccgtga taagaaaagc
    11461 agcttgaaac gttcctgaac caaatatcgt gaaatttaaa gtgttcgaca acatttcaag
    11521 caaaccagcc cagatcggtc catatagata acccataagc gccatactta aaaatccgaa
    11581 tgtgatttga aaataagcag gaccaatcgc tatcttgtat aagatgacat caagggctaa
    11641 taaaagagca gaggtcgaca acaatcttgc attcatttcg ggcaatctaa aaacgtcttt
    11701 tgcaatcaaa gtgttcaaaa tattctctcc tttgtcagtt aataataaaa ggataccata
    11761 aaatcaccaa tgtgtcatga cacattggtg atttacatac actggcatct aacaattaaa
    11821 aaaagggtga gaagactgct tttataaact gtaaaaaaga gattgtacga ataaaaaaaa
    11881 tagaggcgga gaatacttat cgtatcatcc gcctttgttt ttttgttata aaaaacgtca
    11941 gattagttat ctgcttacat cttcatccat acgggttcta atgtatttcc gtctagatct
    12001 tggacttcta atgaaaacat ttcttcttca ggcattccca tatctacttc gtggaaagta
    12061 ccaccatttt cttttgcaat ttgagcaaat ttttttactt catccgcgtt ttcaacacta
    12121 aacgaaatca aagcaccact tgttttttta ttatcagcta tttctttatc ttttataaat
    12181 tttttataaa aatcatgaga gagaagcatt atccaaaaag tgtcatccca taccattgaa
    12241 ccagcttctt cagtagaaaa ttcttcattt tttttaaaac ccagcttttc ataaaaagca
    12301 acggaacgat tgacatcagc aacaggaaaa ttaacaaaaa ccatactatt catttgtttc
    12361 gtctcctttc tgttttgttc gtctatcatc tcttatttag gaaacgctgt caaaaattta
    12421 gattcatata tagaaaccaa aataaaaatt tgcatcatca aaaaagtgaa gatgtgatag
    12481 aatgaggact ggttcacttt cacttaacta atagatggaa tttctttata ataaaaaagt
    12541 gaatttatat tgaaaaagaa aaaaacaagt aaagccgggg aatattccgg tatgaaagga
    12601 atggagagaa caatgaccat ccaatggttt ccgggacata tggcaaaagc ccgacgagaa
    12661 gtttctgaaa aaatgaaata tgtggacatc gtatttgaat tggtagacgc acgattacct
    12721 ttatcttctc gtaatccaat gctagatcag atcattcagc aaaaacctcg ccttgtttta
    12781 ttgaataaag cggatctggc agatccacaa cagacaaagc tgtggcaaga atattttcgt
    12841 aaacaaggcc atcttgcatt agctatcacg gcacaagaaa gtaaaggaat taaaggtcta
    12901 gtacctgcgg ctaaagaagc attgaaagaa aaacgggcaa gagatgctgc tcgggggatc
    12961 aagcctcgcg ctattcgggc aatggtctta ggtattccaa atgttgggaa atctacgttg
    13021 atgaatcgac tagtaggcaa gaaaattgca cagacaggaa ataagcctgg cgtaacaaaa
    13081 ggtcagcagt ggcttcgttt aggtaatgag ttggaattgt tagacacacc agggatttta
    13141 tggcccaaat ttgaagatga agaaatcggc aaaaaactag cgctgacagg cgcgatcaaa
    13201 gaccagcttg tccatttaga tgatttagcg atttacggat tagacttttt tgcccgatat
    13261 tattcaggaa gaatcagcga tcgttatggt ttatcaatcg agcaagaaga aaaattggca
    13321 ccggaattac tgatggatat tacagcaaaa cgtgggtata aagaagacta tgatcgtggc
    13381 agcgaattag tcgtgcatga tattcgtcaa ggaaaacttg gacgatacac actggatcgt
    13441 tgcgaagaaa tggagcaaaa aagtgatgac tgaatccatc caaagtatca aagaacgatt
    13501 gaaaagcata cagagaatag atgatcctac actagaatca ttaagaaatg atccacgcaa
    13561 aggcgtgcag caagcactgg cttcttttga acgacgattg aaaaaggaag cattaattaa
    13621 ggaaaaatat gaagaaatgt tttgctttga aaagcaaggg tacgcacaag gtcaccaatg
    13681 gattgccggc attgatgaag taggaagagg accattagct ggacctgtcg tggcagctgc
    13741 agttatcttg ccgaaaaatg agatcatatt agggctgaat gactccaaac aattatcaga
    13801 gaaaaagcga gaacttcttt atcaacaagt tcaagaaaaa gccctggcaa tcgggatcgg
    13861 gatcgttgat cagcagacaa tcgatgatgt gaatatttat gaagcaagta aattagcgat
    13921 gatccaagca gtagaaaaat tgtccattgt accaagctat ttgttgatcg atgcgatgac
    13981 cttggatttg cctattcagc aggaaaaaat aatcaaaggg gatgcacgat ctgtgtcgat
    14041 tgctgctgca agtatcattg ctaaagtata tcgagaccaa ttgatgaaag aatatgacgt
    14101 gctttatcct ggatatggtt tctcgcataa tgcaggttat ggaacaaagg aacacttaga
    14161 aggacttgaa cgacaaggaa ttacgccaat ccatcgtaaa agttttgcac ctgtaaaaaa
    14221 atatttacaa taaaaaacag ccgcttttac gtatcttatt agaaagagag acgaaaggcg
    14281 gttttatgat gtatcaaata gaagaaaatt tattgaaact tgcttataca aaaggaatca
    14341 gtagtcaagg aaaatgggca cttgctaact ggatgatgca gtatccttat aaagaaatcg
    14401 gatttgaaga catcattaag ataggtaaaa taaccaaaca tcgggagctg tttcgcaaaa
    14461 gttggcagga aattcacgct aactggggca aaatccaatc gaaacagtcg tttatcactt
    14521 gctttgatcc caaatatccc cctcagctgc ttcacttgac ctatccgcca attgtactgt
    14581 tttataatgg tgatctatct ttactttctt gtaacatgtt aagcgttgtt ggtggaagac
    14641 acacttctgc tttagccaaa aaaacagttt attatttact aaaaccagtg atagaagctg
    14701 gatatgtgat cgtcagtggc tgcgcgaaag ggatagatac atacggtcac caagcagcaa
    14761 tctctcatgg tggacggaca atcgcagtca ttggaacagg gatagagcaa gtctatcctg
    14821 ctgaaaatcg tgacttgcaa gaggtgattg caaatgatca tcttcttttg agtgaatatc
    14881 ctcctcaatc cggcccgaaa aagcattgtt ttccaatgag aaatcggatt atcgcagctt
    14941 tgtctaaagg aacatgtgtc tttgaagcca agaaaaaaag cggctctctg ataacagcac
    15001 agcaagcatt ggatcttgga agaacagttt tttctgttcc aggaaatatt ttaacagaac
    15061 attctaccgg atgtcatcat ttgatccaag acggtgcagt ttgcacggtt tcgggtgaag
    15121 atatcttggc agaatttaaa gaataacacc atacttttca tttgcttttc ttgacaaacg
    15181 ttttctgaat ggatatgatt agctacgatt tgtatgaatc ctcttttaat agtaggaaca
    15241 cgacaatgga aaggagtcca ttcagtaatg gcgtataaat atttagtgat tgtagaatca
    15301 cctgcaaaag ccaagacaat tgagaaatat cttggacgaa actataaagt aatggccagt
    15361 gttggccata taagagattt accgaaaagt aaaatgggta tcgattttga aaacaattat
    15421 gaaccccatt atatttctat acgcggaaaa ggcgatgtca tcaaaagcct gaaagccgca
    15481 gcaaaaaaag ctcaaaaagt ttacttggca agtgacccgg atagagaagg agaagcgatt
    15541 gcttggcatt tagcgtacct acttgggttg gatctgaaag aaaaaaatcg ggtggtcttc
    15601 aatgaaatca caaaagacgc agtcaaagca gcttttaagg aaccaagaac gatcgatgta
    15661 gatttagtag atgcacagca agctcgtcgt accttagaca gaatcgttgg ttattcgatc
    15721 agtcctattc tctggcgtaa ggtcaagaaa gggttaagtg caggacgtgt ccaatctgtc
    15781 gctttaaaaa ttattattga ccgtgaaaaa gagatccgag aatttgttcc agaagaatat
    15841 tggagcatcg acggtaattt taaaaaagct cgcaagaaat tcaaagcaaa tttctgggga
    15901 atcgacggta agaaaaagaa attaccagat gcacaaagtg taaaagaagt cactgctaga
    15961 attgatggaa cagacttcga agttaaaaaa gtagaaaaga aagagcgcaa aagaaaccct
    16021 gcgaatcctt tcactacaag tagcctgcaa caagaagctg cccgtaagtt aaactttcga
    16081 acaagaaaaa ccatgatggt agcacagcaa ttatatgaag gaatcacttt gggtaaacaa
    16141 ggaacagtcg gcttgattac atacatgcgt actgattcta cccggattgc cgatacagca
    16201 aaagcagagg ccgcagcatt tattgaagat acgtatggaa aagaattttc tagacatgat
    16261 caccgaaaga tcaaaaatgc tcaaggcgca caagatgccc atgaagctgt gcgtccaacg
    16321 agtgtgcttc gtacaccaga tgaactcaaa caatatttag ataaagatca attgaaactt
    16381 tatacattga tctggtcacg ttttgtggct agtcagatga caccagctat tttagataca
    16441 atgaaagtga cattagaaca aaatggtgtc atcttcattg cgaacggttc aaaaatcaaa
    16501 ttcaaaggat tcatgcaagt gtatgtggaa ggtagagatg acggcaaaga agagaaagaa
    16561 aatattcttc cagaacttga agaaggcgat gtagtccaat ctgttgatat tgaaccaaaa
    16621 caacatttta ctcaaccacc agcacgtttt agtgaggcta cgttgattcg tgcactagaa
    16681 gaaaacgggg tagggcgacc ttccacttat gcaccaacac ttgatacgat ccaaagacgc
    16741 tactatgtaa aattgacaca aaaaagattt gagccgactg aactaggaga aattgtcaat
    16801 tcattaatct gtgaattctt ccctcaaatc gtcgatatcc atttcacagc agaaatggaa
    16861 ggagatttgg ataaaattga agaaggcacg gaagcgtggg tcaaagtggt tgatcgtttc
    16921 tataaaccgt ttgaaaaaga actgacgaat gcggaagaaa aaatcgaaaa aattcaaatc
    16981 aaagatgaac cagcaggatt cgattgtgat gtctgtggac atccgatggt gataaaatta
    17041 gggaaatatg ggaagtttta tgcttgcagt aattttcctg attgtcggaa tacgaaacca
    17101 atcgtaaaag aaatcggtgt gacttgccca gtatgtcatg aaggacaagt cattgaacga
    17161 aaatctaaga aaaacagact attctatggt tgttcccgtt tcccagaatg tgaattcact
    17221 tcttgggata aaccagtggg cagaaattgt ccaaaatgtg acggatatct ggtcgagaag
    17281 aaagtcaaag gcggtaaaca agtagtctgt gtaaatggag attacgaaga agacatccaa
    17341 aaataataga aaagaggttc tttcattgaa aatggtaaat gttgttggag cagggcttgc
    17401 cggaagtgaa gctgcttggc agatcgcaca agcaggagtc cctgtccgtt tatatgaaat
    17461 gcgtccagtc aaatcgactg aggcgcatca cacaagtaat tttgctgaac tagtatgttc
    17521 caattcatta agaggaaaca gtttggctaa tgcagtaggg ttattgaaag aagaaatgcg
    17581 ccgattgaat tcggtagtaa tcacttcggc agatgaaaca gcagtgccag ctggtggtgc
    17641 cttagctgtt gaccgcgatt ctttttctga aacaattacc cgaaaagtca aagagcatcc
    17701 attagtaaca gtgataaacg aagaaattac tgaaataccg gaaggaatca ccgtagtagc
    17761 aacaggacct ttgacttctc atccattagc agaaagcatc aaagcattca atggttcaga
    17821 tggtttttat ttctatgatg cagctgcacc aatcgtagac aaagctacga tcgatatgga
    17881 taaagtatat ttgaagtccc gctatgacaa aggagaagca gcttacttga actgtccgat
    17941 gacagaagag gaattcaaag ctttccatga ggcactaatc aatgccgaag tggcagaact
    18001 taaatctttt gagaaagaaa aatattttga aggatgtatg ccaattgaag taatggcaca
    18061 gcgaggattc aaaacgatgc ttttcggacc tatgaaacca gttggattgg aagatcctaa
    18121 aactggcaaa cgtccttatg ctgttatcca gctgcgtcaa gacaatgctg cagcttcttt
    18181 atacaacatc gtgggctttc agactcactt gaaatggggc gagcaaaaac gtgttttccg
    18241 gatgattcct gggttagaaa atgctgaatt cgttcgctat ggtgtgatgc accgaaacag
    18301 ctttatgaat tcgccagaat tgctgaaacc gacttaccaa tcaacgaaac gcgaagatct
    18361 gttctttgca ggtcagatga ctggtgtaga aggctatgtt gaaagtgcag cgagcggact
    18421 acttgctgga atcaacgccg cacgcttagc aaaagaaatg gaaccagtcg agtttccaca
    18481 agaaacagct attggaagta tggcttacta cattactcat gcggaaggaa aacatttcca
    18541 accgatgaat gccaactatg gtctgtttcc tgaactgcca gagcgcattc gagacaaaaa
    18601 agcaagatat gaagcaattg cacaacgagc tttaaaagct aatgaggcaa taaaaacaga
    18661 attagaaaaa actgcaatcc caaatgagta aggaaactcc atgaagaggg tgagacggat
    18721 agtctcgctc tttttctggc gtttttctta agaatttctc aaacaaaatg tgaggaattg
    18781 cgccaattgt gaacaaactt agaaatattg gttaaattct gacaatttat ttgtcttttt
    18841 ataaacgctg tgctaaatta ttctctggga caaaaggaag gtgagaaaat gacagaagcc
    18901 acgtgggaac aaaaattttt gcgttatttg attgtcgaaa gaggatattc agaaaaaacg
    18961 aaagaagcat atgaagaaga cattctccat ttcaaacatt ttttggagga atctggagac
    19021 gctgatctgc tgaaggcgga atattttacg gttcgtgtct atttgagtgc attagctgat
    19081 catcgtccta gctatagcag aaattctatc agtcgcaaga tagccagcct acgttcgttt
    19141 tatcagtatc ttctcaaaga agaagtgata aaagaaaatc cattttctta cgttcatctc
    19201 aaaaagaaaa atttgagact gccacgtttt ttttatgaaa atgaaatgca agcacttttt
    19261 gacagcgtag caggagatag tcctttggat ttgaggaacc gcgctttact ggaagtcctt
    19321 tacgggtcag gcatccgttt aagtgagtgt agcagtttgt cggtatcaga cattgatttt
    19381 gattcagagg tcatgttgat ccatggtaaa gggaacaaag agagatatgc tcctctaggt
    19441 tcttttgctc aagatgctct gcaagaatat ttcagggaag gccgccaggt attgatgaca
    19501 aaatatcacg aagagcatga tatcgttttt gtgaatcatc atggaaaacc aatcacgcct
    19561 actggaattg aatatgtctt gaatcaaatg atcaaaaaaa gcagtttgga tagtgatatc
    19621 catcctcaca tgctgcgcca tacatttgct actcatttat taaataatgg ggcagatttg
    19681 cggacagtcc aagaattgct aggacatgcc aatttatcga ctacccagat ctatgcccat
    19741 gtaacaaaag aaagtttaca aaaaaactat cgttctttcc atccaagagc gtaaaaataa
    19801 ggaaaagaac a
//
LOCUS       DACDEG010000043        19480 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.045, whole genome shotgun sequence.
ACCESSION   DACDEG010000043 DACDEG010000000
VERSION     DACDEG010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.045"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(70..1050)
                     /locus_tag="IV142_001581"
     CDS             complement(70..1050)
                     /locus_tag="IV142_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8485246.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(1074..2558)
                     /gene="galT"
                     /locus_tag="IV142_001582"
     CDS             complement(1074..2558)
                     /gene="galT"
                     /locus_tag="IV142_001582"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP8485247.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(2570..3559)
                     /gene="galE"
                     /locus_tag="IV142_001583"
     CDS             complement(2570..3559)
                     /gene="galE"
                     /locus_tag="IV142_001583"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP8485248.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            3807..3974
                     /locus_tag="IV142_001584"
     CDS             3807..3974
                     /locus_tag="IV142_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP8485249.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(4036..5847)
                     /locus_tag="IV142_001585"
     CDS             complement(4036..5847)
                     /locus_tag="IV142_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP8485250.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(5844..6209)
                     /locus_tag="IV142_001586"
     CDS             complement(5844..6209)
                     /locus_tag="IV142_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP8485251.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(6372..6767)
                     /locus_tag="IV142_001587"
     CDS             complement(6372..6767)
                     /locus_tag="IV142_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8485252.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(6785..7747)
                     /locus_tag="IV142_001588"
     CDS             complement(6785..7747)
                     /locus_tag="IV142_001588"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP8485253.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(7747..7959)
                     /locus_tag="IV142_001589"
     CDS             complement(7747..7959)
                     /locus_tag="IV142_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP8485254.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(7980..8678)
                     /locus_tag="IV142_001590"
     CDS             complement(7980..8678)
                     /locus_tag="IV142_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP8485255.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(8698..9240)
                     /locus_tag="IV142_001591"
     CDS             complement(8698..9240)
                     /locus_tag="IV142_001591"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP8485256.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(9372..10376)
                     /locus_tag="IV142_001592"
     CDS             complement(9372..10376)
                     /locus_tag="IV142_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP8485257.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(10373..11362)
                     /locus_tag="IV142_001593"
     CDS             complement(10373..11362)
                     /locus_tag="IV142_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP8485258.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(11359..12165)
                     /locus_tag="IV142_001594"
     CDS             complement(11359..12165)
                     /locus_tag="IV142_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8485259.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            12331..13287
                     /locus_tag="IV142_001595"
     CDS             12331..13287
                     /locus_tag="IV142_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8485260.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVSSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(13364..13882)
                     /locus_tag="IV142_001596"
     CDS             complement(13364..13882)
                     /locus_tag="IV142_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8485261.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(13970..14119)
                     /gene="rpmG"
                     /locus_tag="IV142_001597"
     CDS             complement(13970..14119)
                     /gene="rpmG"
                     /locus_tag="IV142_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8485262.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            14347..14793
                     /locus_tag="IV142_001598"
     CDS             14347..14793
                     /locus_tag="IV142_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP8485263.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(14988..16883)
                     /gene="asnB"
                     /locus_tag="IV142_001599"
     CDS             complement(14988..16883)
                     /gene="asnB"
                     /locus_tag="IV142_001599"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP8485264.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(17208..18182)
                     /locus_tag="IV142_001600"
     CDS             complement(17208..18182)
                     /locus_tag="IV142_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP8485265.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            complement(18516..18603)
                     /locus_tag="IV142_001601"
     tRNA            complement(18516..18603)
                     /locus_tag="IV142_001601"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18569..18571),aa:Ser,seq:cga)
     gene            complement(18748..19314)
                     /locus_tag="IV142_001602"
     CDS             complement(18748..19314)
                     /locus_tag="IV142_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8485266.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
BASE COUNT         5890 a         4098 c         3306 g         6186 t
ORIGIN      
        1 aattttgccc cttcctgaaa cacaaaaaag ctgcggtatc acttcatttc cccacagctt
       61 ttccatcttt tatttcgttg attctcgaat aatcaactct gtttccacaa taatctttct
      121 tgaaaccggt gctttggaat tacttaattg aagaatcgtc tccactgcta gctctcccat
      181 ccattcggta taaactttta cagtagaaag agagggcgtt acatatttcg ctacactgac
      241 atcattgaaa cccatcacag aaatatcttc tggtaccact aaaccttttt cttgaatcgc
      301 tttcagtgcg ccaatcgcta atgcatcatt agctgcaaaa agcgcatcag gaacatgcgg
      361 attcgttttt aaatactgct tcaccgcttg atatcctgac tcgacagaaa aatctgcttc
      421 gataattgga ccttcgtcgt agcctttttc tgccaaaact tgttgaaatg ccataattct
      481 gggatctttt agccgctgat ggtttttctt cgtatattct gttccagcaa gcatagcgat
      541 atgttgcgct ttatgggaga caaaatgttc aatgactttt tgaacacttg acttaaagtc
      601 gacaactaga gagtcgattc cttgagaagt gccgtcagaa tctaccaaaa gcaaggcatt
      661 ttgggatcct ttcatttcta ataactgagc ttcgtcgaat tttcctagtg cgatcgttcc
      721 atctgtcgga agatcactaa tggaatccca cgtttctttc aataaagtaa cgtttgcttc
      781 ttctgccttt ttttcgattc ctagacgaat agataaataa taaagatctt caagctcttc
      841 cgtatcgtcg taccactgga tcaggcgaag cgtttgctta gtatttgctg catttttacg
      901 atgtttcgta taatttaagc gttctgctgc ttcaaaaatt ttttgctttg tggctaagcc
      961 aacggaaaga tcaggatcat agttgagaac tcttgaaaca gtagcaggag aaactcctgc
     1021 caactgcgca atgtctttaa ttgttgccat ttgtctctcc tcctttttta cgtttataaa
     1081 attgcagtaa atcgttcaaa cgctgcctgt cctttttcat ctcgcttgaa tactccagcg
     1141 tcttccagta cacgtgcaaa aacctgacct acagattttt gtacgacatc ggtgaccgta
     1201 tctttcgtca aatcaggatg ttcttttttt aattgttctg cccacggacg atgatattca
     1261 gccatctggt tttcttctcc taataagaaa tgctctactt ctactagttc tggtttcaaa
     1321 cgaggtggta aaacagcaag ccccattact tcgatcaacc caatgttctc ctttttgata
     1381 tgttggacat ctttatgtgg atgaaaaatc ccatcaggat gttcttctga aacattgttg
     1441 tctcgaagga caaggtccaa ctcaaagtaa ttgtcccttc ttcgtgcaat tggcgtaatc
     1501 gtatggtgtg gtgtaccatc tgctgaaaaa gctctgatag atactgtttc atccgaatat
     1561 gctttccact tttccaatac tgagttagct gcttcaatca acaatttttt ctctgttgat
     1621 tgcaaacgga tcacagacat cggccatttc acgactcctg cgatgacttg cggataatct
     1681 gctagtttga taagtttttc tatcggtgct tttgccatag gaaattcatg acgtcctgcc
     1741 tgataatgat catgagaaag aatcgatcct ccaacaatcg gcaagtcagc attcgaacca
     1801 acaaagtaat gaggcaagac ttcagtaatc cgaagcagtc tagcaaatgt ttcctttgtg
     1861 attttcatcg ggcggtgttg ttctgataaa atgatcgaat gttcattgta ataagcataa
     1921 ggagaatatt ggaatcccca gctttcaccg tctagattca ttcgaataat ccggtgattg
     1981 gtgcgggctg gatagttcaa gcgacctcta tatccttcgt tttccatgca aagcatgcag
     2041 gtaggatagt ctacttttac cgcctcgcgt tctgctgcga tttgttttgg atctttttct
     2101 ggcttagata aattgattgt gatttccagt tcaccatact ccgactctgc cggaaatttg
     2161 atgttttttg cgatggctct tgttttgatg tagtcatttt tcttacttaa attaaagaaa
     2221 tactcagttg cttcctctgg gcttttcgaa tagtgctgtg caaagaatgc attgacgact
     2281 gaaggtggcg gtgtgagaaa atccattaat tgcgcttcaa aaatctcttg ttctgctggc
     2341 aattctgaaa gaacaccatt ttctcttgcc ttagcgacaa gctgatccaa taagtctaca
     2401 gattcttcct taactggtct tacttcaatg ctttctagag catcttctcc taccatccct
     2461 aaaagacgat tctgtaaata taagcggtcc atttccatcc atccgccagc ttcgatcgca
     2521 agtgtggcaa aatcacaaat cgtttgactg attgacatat ctcattcccc tattcttcat
     2581 aaccatttgg atggctgaca tgccaatccc atgctgtttt gataattgct ttgatatctg
     2641 tgtattcagg tttccaaccc agaacctctc tcgctttttc acttgaagcg actaatcggc
     2701 taggatctcc agctcttctt ggtgctattt ctgctggaat ttcttttcct gtcacttctc
     2761 tagctgcttc tagcatttcc ttgactgagt atcctttgct gctgcctaaa ttgaagaagt
     2821 tgctttcatt tccttctttc aagtattcca aagctaaaat atgtgcagca atcaaatctt
     2881 ctacttgaac ataatctctg atacatgttc catctggtgt atcataatcg tctccataaa
     2941 cagccaaggc ttttctttga ccaagagcta cttgtaaaat gattgggaca agatgcgttt
     3001 ctggtgtatg atcttctccg atagatgcat ctgcttttgc tccggctaca ttgaagtagc
     3061 gcaaagcgac atagcgcatg ccatatgctt ggtcacacca cttcatcatt ttttccatca
     3121 tcaatttact ttcaccatac ggatttttag gattagctgg tgtatcttct gtgattggag
     3181 actctttcgg ctctccataa gttgctgcag tagaagagaa tacgatttta ttcacgttga
     3241 actcgtgcat gacttcaagt aagatttgca taccgtatac gttgttgttg aaatacatca
     3301 atggtttttc gacagattct cctactaaag agcttgcagc aaaatggatt actccttcaa
     3361 tcggttcctt ttcaaatacg gaacgtagaa attctttatc ccggatgtct ccttcataaa
     3421 aatgagcatc tggatgtact gcttgtttgt gacctgtgag caaattatct accacagcta
     3481 cttgataccc cttttgaact agctggtcta cagcgtgcga accgatataa ccggctccgc
     3541 ctagtactaa aattgtcata aaaaacactc ctttgattag taaaattgtg tttgttgatt
     3601 ctatataatt taaaaaacga agtttacttt tatggtgaaa acaaacaaag taaaatttcg
     3661 taaattcata tggtaaattt ataataaaac atttagtaaa cattttcaag gattttttgc
     3721 aaaggtttac aaacaaacgt aaaactagcc tttttttctc ttttgttcca taatagatat
     3781 aacaaataat aaatgaggtg gattcaatga aaaaatttgt atctggaata ttagtaggaa
     3841 gtctggcaac agccgctgcc gttgctggtt tggttgcttc tgtgaaaaaa acagtgatcg
     3901 atccaattga cgaaaaagaa gcaatgatcg aagaaaaccg taaaaaagca atgcgtaaac
     3961 gcgtttctcg ttaataatta tttaaaaata cgcatgaaga ctttctaaaa aacagataag
     4021 tcatcatgcg tatttttatt tgtcagtcaa tataagttgt gctattttat ctgcctgtct
     4081 cagattccat ggatctgtat aaggtctagc tactgctgca gttagccaat ccacaccttc
     4141 aacaggtatt ccatagcaat aaaagtcaga ctggatttcc cctgaagcat caatcacgcg
     4201 attcgtacct cgctcaacat ctatagcttc tgaatgataa cttcctgtat tatctgaaaa
     4261 tgaaaaaggg cgaacaagtt tgttttcctt caaatttttt atgacaggat tacttgaacg
     4321 agaaaagctt gtttgtggca atcgagcttc aatcaaatag cgactgctga tttctttatc
     4381 ttgtagagaa ataacatagc ggttgtgctt ttgctgaata tcaaatggcg gttcgatcaa
     4441 atgaaaaata cccgcatcta tcagtgctgc taattctttc gtccgaatga ttggcggacc
     4501 gatcgttaaa aagctattta gatgagtaaa ccatccccat agtatttctt tgtattcctt
     4561 agcagaaaaa acgtgccatt caatagcctg ccggattgga tcacgccagt cttttaatgc
     4621 atcaaatgtt gaagcaaatg ctcctgtgca attgcctttt tccgcttctt ttatctgccc
     4681 ttcaataaat attcggctag cttctgaaaa gggctcattc tgataggcaa gaggagtttc
     4741 taaatacgac caatcccatc tataagggat tagtgctgga tgctttccga gacactcctc
     4801 tgaagagagg gagagaaaat catgctcaaa atttttcttt ggaatcggca agtgatattc
     4861 ttcgatgatt ttcttgtaat aaaacaactc aacgtctttt ttcagatagt caaaaaatgt
     4921 ttcgccagtc aaaaatcccg actgatgcca ttgctccagg ttttcttttg ttagtaaccg
     4981 tggccatgcc attgctccgc cttgcttttg atttcttcct cgagggtaat aagggagccc
     5041 ccttcttgat ccactgtata caatcggttc cttgcctgat ggatgataga ccagttttcc
     5101 cttttcgttc tcgaagcgcc ctcctcttga tactgtaaac aatccgacat aatcaaaaaa
     5161 acttaagcct agacctctta aaaagacatt ttctttaggc ggtatcttcg aaagatctac
     5221 atctgcagga ttagaaggaa gctgatacag taaaacattt ttctttgcgt agtctaccca
     5281 cactctttct tcttctgtct catcattttc ccagtgaccg cttgctaaaa caagctgatc
     5341 gatagaatag ctttgctgat ctgtctgtaa aagataacca tcttttcttt tgcttacttt
     5401 ttttactaaa gaataaacaa actgaataga agctggaaat tcttcttgca actttgagaa
     5461 aaaccagcgt tgatagagtc cgtataaaca tcgagtagat tgctgatctt tttccagacg
     5521 attgatttct tctataatta gttgcttatt tttggttacc gtctgttcga tataatctgt
     5581 agcaggtcct ttactccatt gaaacaagtt gggtccctgt gttaccaatc ctccactgga
     5641 caatgtttca tctgtaaaaa gcgtcacttg ttgagaaaca gaattcatta ataactccgg
     5701 tggctgattc aaccgccaaa ctcgacctcc aggaccattg ggatcgaaaa gaaaaagttc
     5761 aagagaatgt ctgtcctgat tattatggag aatcctttct gctacactta atcctctagg
     5821 tccggcacca atgatcccta ttttcatttg catgcctcca tttcgataaa ttttttcaat
     5881 aaaacagcat ggttctgatg ttcatttttt gcaccataaa caaaagtaat gactggatgt
     5941 tctttcataa gttgactgag tttaaaaata gcttgcttct tttcttcttc gaaaatcaat
     6001 tcttgctggt atttctttgc aaatgaagca aatcgttctg gtacatggtc aaaagttttg
     6061 cgtgcttcat tactaggtgc aatttctttc aaccacagat ctaactgcgc tttttctttt
     6121 gaaattcctc gtggccagaa tctatccacg agtattctat agccatcctc tttttcagca
     6181 tcaaggtaaa cacgttttaa tttgatcata gttgtttcct tctttctacc ttttcgctta
     6241 gcttatatat gtagataata gcacaaaata aaaaaaatga acggcacaac tatccattgc
     6301 gccgttcatg tgatcttggt caaaatctaa tgattgattc ggtttttaaa gctgtcatac
     6361 aatcatctgt tttatacttt tttacttttg atttttccag accatccgtc atatccaccc
     6421 ttaagtaaat aaatctcttg ataacctgct ttacgtaatt tatttgctgt acggatgctc
     6481 aaacttttct tttgatcgta aatatagaca ggctggtctt ttcgaataga tcctaatgaa
     6541 tctttcaaaa cagtgtaagg gatgtttctt gcacctaaaa tatgcccagc atcaaacaca
     6601 tctttttcgc gaacatcgat tacttgtgct tttctcattc cttctcgaaa ttcttcttct
     6661 gtcaaagtcg ttgctgaacg tttggccatt actcgtagat agacttccca aaggatgatt
     6721 gcaagcacaa tcaatagcaa aatgatatta attacccata aaaccatgta atgcctccgt
     6781 ttttttaagc aaatttcaag gctaatgagg cagcaccaat gacaccagct tcattaccaa
     6841 gctctgctaa tttgattttt gttgattctc ttacttgcgg gaaagtaaat tctttaaaat
     6901 atttctctac acggctacgt aaaaattctc ctgctgccga tactcctcca cctaacacga
     6961 tacttgacgg atttaatgta ttccccaagt ttccgcaagc taaacctaag tagaagcata
     7021 cgcgatcaac aaccatcaat gcaaacggat cgtcttcttg agccatttca aagatatctt
     7081 tacttgttac ttcttctccg ttgtctagca ttgctttcag ttttgaatcc cctgcgtatt
     7141 cttctgccaa gaaacgacct aggcgtacca cacctgtagc gctagataca gtttctaaac
     7201 atccgcgttt accgcaagta cattcgaacc cttcaggatc cacagtgata tgtccgattt
     7261 ctcctccagc tccgctcacg ccgtgaatca aatttccgcc agcaacgata cctccgccaa
     7321 cacctgttcc tagagtaacg aatacaacgt ctggatcgtt ttctccagca cctttccatc
     7381 gttcacctaa agcagcaaca ttcgcgtcat tatcgatagc gaatttgatt cctgttcctg
     7441 cctcgatcag ttttttcaca aattgaactt ctgtccagtt taagttatac gcaccaataa
     7501 ccgttccagc ttcgctgtcc acgcttcctg gggtacccat gccgattccg ataaagtctt
     7561 cggctttcat attatataaa ttcaaacgat gattgattga ttcaatgatt tcaggaacga
     7621 tatgcttacc atcatccaaa atattggtat cgatgctcca tttttgctga atctctcctt
     7681 ctggtgttaa aattgcaaat tttgctgttg ttccacctaa atcaatccca ataatcttct
     7741 tgtccattac aaatttcctt tctttcggct ttcttcaatt cggtgctctc tttttaatat
     7801 gcgccacgct gtcatgtatg tatcatgatc aatcaaacgg ctatcataca gattacgcaa
     7861 ttcgatagtc attaattcaa tgtcatagat gcgtgcaccc acatagataa agatgccaaa
     7921 ttttttgaac agctgctgta catcatataa gctctccatc tttaagcact tccttcatat
     7981 tatacaagta aaccatattt ttttaaaccc agtaaaatac atattacaaa aagaaataca
     8041 aaaactagtg tagaaagaat cctttggtgt atcgaatagc ttgtctgttg tttcggtaga
     8101 cccaaaacat tccctaacac taatccgcca attagcccac cgacatgtcc ccaaacatct
     8161 acagaggtat ccagaagacc aaaaacaaat gtcaacacaa taaataaaag aaaatgtctg
     8221 actagctgct ggatcgctgg gttatgttta aaatgaaacc ctaagataaa caacgcaccg
     8281 aacaaaccga atatcgaagt acttgcacca ccagacagaa cattcgcttc gttaaaagca
     8341 aagcttgctg tatttcccat tatcccgctg aacatataaa tcaagaaaaa acgccaatgg
     8401 ccatagattg cctcgatttg ctgccccata aaatacagta tcagtgagtt cattacgaaa
     8461 tgcatcagtc caaaatggat gaagattggt gtcactaaac gccaccattc attgaagtga
     8521 acaagataag gaccatacat tccggcataa taagggatct caagtcgcgg aaataattcc
     8581 atcatgagga agaccgctgt ctgtatccct aaaaaagcat aggtccataa cggtcgtctt
     8641 aaccatgcac gcaattttat ttgctgttga taattcatga aaacctccca tttcatttta
     8701 gtcaataaac aattgttgga tcttctgatc aaagctctcc ggttgccatt gttcattcaa
     8761 ttgttcacga aaaacaaggc tacatgtttt tttatgaaaa ttatttaaat accggtcgta
     8821 ataaccaccg ccaaaaccga ttcgataacc ggctctattg tagataatac ctggtacaat
     8881 gaggagatcg atcatctccg gcaaatagag cttctctgat tgtggctcaa gaacaccaaa
     8941 actcgaacgt acaagaacgg actgttcatc catttcataa aacgccatct gacgtttcgg
     9001 caaagtttta ggtacaacta ttttcttttg ttctttttct gctcgttgga taaccggctg
     9061 tgtatccagt tcaaattcat tcgataaagt cgtccctatc acttttgctg acttccagta
     9121 ctcgctggcg aatagctgtt tatagattcg ctgttctttt tcctttttct tgccactttt
     9181 ggccagattt ttcagctttt cgattccttt ccggcgtaat cttactttca attggtccaa
     9241 aaactcaccc ttccttctct cataaaatgg tataggaaaa aaagaaaaat agcaatcgaa
     9301 ctttcaaata aatgcataaa tagaataaaa agaagaccta gaaatgattg gccttccttt
     9361 tttaatgaat ttcaactgtt cagcgcgtgt ttttgtctga taaaaagtgc atacagaaaa
     9421 tatggcgcac caaaaagggc aatcaaaatt cctgtaggta cttccccact tagtagaatc
     9481 actctagcaa ccatatctgc actaagcaga aaaatggaac cgaacaaacc gctcagaaca
     9541 aaggtccagt tattttcttt acgtacgact agcttcgcag catgaggagc tagtagtcct
     9601 acaaaagata aactacctgt caccgctaca ctaccgcttg ccaaagcaac agctaacaac
     9661 aaaaggattt tttgactttt agccacgtta aggcctaatc cttgcgcgcg ttcttgacca
     9721 aatgatagca atacaatcct ctgtgcataa agtcctccta gcggaattaa gataaccgcc
     9781 catggaagca atgcccaaac ataaggccag ctcgcacccc agattgatcc cgctaaccat
     9841 gcattcacaa atccataatt ttccttagat aattggatcg tgcatagcaa tgttaatgca
     9901 gaaaggccgg cattaacggc aataccggac aataagattc gattcatacc caaaaagcca
     9961 tttcgatcat aggccgcaag atagacacag caagcagcta aaacaccacc tagaaaggct
    10021 acaactagca atagaaacgg cagcgaatag tgcgaaaaga aacctaaata aagcatcact
    10081 aaaaatcctg ctccggcatt aatgcccaat atactagaat ctgccaattc atttcttgtt
    10141 actccttgca agatataacc agaaacgcca agacaagctc ctgctaataa actgatcagt
    10201 aatcgaggaa ctcgaaattc tctaacaatc agcgccaaag aagatgattc tttccctatg
    10261 aacgtggcaa aaagctgatc caggtaaata aaaggcgtac caatcattaa actacctata
    10321 gacaggatga caagtacaaa aagcaagaaa tagaacgtcc aatatatttt tttcaccgtt
    10381 gttccctcct tacttgtatc agtaaaaacg gaacgccaat caatgcaatg actaatccaa
    10441 aaggtgtttc aaaaggggga ttgatcatcc tcgctgtaaa atccgccaac ataaccaatc
    10501 caccgccaac taaaaaagag acaggtagaa cataacgata gtccgttcca accattccgc
    10561 gaacaactgg cggaaccaac agcccaataa atgaaacagg tccgactaac gaaacggcaa
    10621 tggctgctaa cactgcaact agacagaaaa caatcaaccg cgttcgttta ggatgacttc
    10681 caagagctaa tgcatgtgtt tcactcaaat gaatcaaaga tattgctttt cctaatgaaa
    10741 aagaaaccaa aacagcaagt atatataaag gaaacaatac tgctaattgt ttccatgtga
    10801 tattggcagc tccgcctaca aaccaaaaag caagatcctg ctgcagatta aagaattggg
    10861 taagtgcttg gctgatagca gtaaaaaaag aactaatagc tacgccagct aaaatcagct
    10921 gtaaaggatt catgcccatt ttcgaaaact gcaaaaaacc aaatacgagc aaagttacag
    10981 caaaagaacc tgccaatgac attagaaaca cagaaaaatc agaagcttta ggccaaaaag
    11041 caaaactaag ggccattgcc aacgaagcac ccgcgttgat tcctagtaag cctgaatctg
    11101 ctaaaggatt tcttgtcact ccttgcatca atgcaccgct caatgcaaaa caactcccta
    11161 caagaaatgc tcccgccaca cgaggcaaac gaatatttcg tatgatttgg tgactttgtt
    11221 ttgatctatc aaaatcaaac aacgcctgcc aaaaatctgc ttttcccaca gtcgctgcgc
    11281 catttgaaag agcaacaaaa aaaataatca aaagaacaaa acagaccaaa agcaggagtc
    11341 tatttttctt catagtcctc ataattgatc ctcctgctct tttggcaaca aatcataggt
    11401 tagaaaaact ggtttatttg ttcttggaag cgtgacaatc gttgcatcaa tctcaaatat
    11461 ttttttcata cactcttttg tgatcacttc ttcaggtgta ccttctgtga tcaacttacc
    11521 gtttttcatt gctaataaat aatctgaaaa acgtgaagca tgattaatgt catgaatcga
    11581 catcaagatc gttgtttgtt tcatttgatt gatttcagtc aataactgta ggatctctaa
    11641 ttggtgtgcc ggatctaaaa atgttgtcgg ttcatccaag atcaagatat ctgtttcctg
    11701 agcaagcgcc atagcgatcc agacacgctg ggtttgtcca ccagacagcg ttgcgatatc
    11761 ccgcatacga aatccagcta gacctgtagc atccaacgcc cactcaattg cttcatgatc
    11821 ttgcgtgtcc atgccactga acagtttttg ataagggtat cgtccgtaag atactacttc
    11881 ttctactgtc atacctgtag ggtgttcact agtttgtgct aataatgcaa ttttttttgc
    11941 aagttctttt gaactgtatg tagagatatc agagttattg acaatgatct ttcctgattt
    12001 cacaggtagt atacgggtaa aacttttcaa taaagtagat tttccacttc catttggccc
    12061 aatcaaacta gtgattttcc cttcaggaat cgtaacagac atgttttcaa tgattttttt
    12121 gttttcgtag ccggctgcaa tgtcagctgc tcttaatctc gtcatttcac ccatcctttc
    12181 gctgaaaatc attctcaatt atgacgctgt ttcaacattt cgtcaagcgc tactttcata
    12241 tctatagcca aatgaagcgg ttgacagcgt tcgaaatatc aggaataata accaatgaga
    12301 aagattctca attatgttag gaggaaagaa atgaaattaa aaacaaaagt attcacatta
    12361 tctagcatat tgctgctggc tggagtactg agcgcttgct ccccctcttc agataaagaa
    12421 acagctacaa ctgccaattc gacgacttca tccaatagta ctcatacagt cacagataca
    12481 cttggacata aagtagagat tccaaacgcg cccaaacgga tcatcggcag ttacttagaa
    12541 gactatctga ttgcactagg ggaaaaacct atcgcccagt ggactgttgg cagtggttcc
    12601 attcaagatt atctgcaaga tgatttaaag gatgtcccaa cgatttctta tgaccttcct
    12661 tatgagaaag tgttaagctt tgagcctgat ttgctgttga tttcttcttc cgctactgtc
    12721 gaaggcggga aatacgaaca atatagcaaa atcgcgccaa cttatgttgt aaaaaacgga
    12781 acagatgtca catgggaaga acaactaaaa gatgtcggca aagctttggc aaaagaaaaa
    12841 gaggcagaaa aaatcatttc cgattatcaa aaatccatca agaaaactcg agaagaacta
    12901 gcagataaaa tcgagaacaa gacagctgca gtgttgtggg tgaccaacaa ctcagccttt
    12961 atggtcagca aaaatcggtc aagtggaaga atcgtatacg acgatctgga atttggtgtt
    13021 cctgatctag tggaagaggt ctccaaaaaa gccacttctg actggtctgc tgtttcctct
    13081 gaaaagctag ctgaattaga tgctgactac atcatccttg tgagcagtga caaagatgct
    13141 gccatgttca atgaaccaag ctggaagaat ctaaaagcag tgacagacaa tcatgtactg
    13201 caattcggac ctgaatctag ttggctatat aacggaccta ttgccagtca ggaaatggtc
    13261 aatgatatca aagaaaaact aaattgattt taactaaaaa aagcatcttc acgattagtc
    13321 gtgagatgct ttttttctta ttaacaaact agacgcatag attttattct tcttttgaag
    13381 caaccctatt ttttactctt tcccattttt gaaaacgatg ggcatataga ttttcttgat
    13441 ccgttatccc ctcatactcc tctataaggg cgaagtctga aaaatcaata ttcccaatat
    13501 acgtatcgcc ttcaaacgtg ctatcgatca atgttcgcca gatccgacca gtgtaaggaa
    13561 gaagttcttg aaaaatccga ctaccacccg aaacatagat atcttctgag atcgtttgac
    13621 tatacgacaa aagttcatcg atcgaatgca ttacttccgc attttctttc gtgcgaaaat
    13681 ttggttgcgt tgtcaacacg atgatatggc gataaggaag tgaaagatcc cccattcctt
    13741 cataggtctt tcttcccatc actaaaattc gatccattgt gtgttcgcga aagaaacgca
    13801 tatcatttgg caatcgccat ggtaacaaac catttttgcc gatcagacca tttttatctt
    13861 gtgcccacat ggaaataaac atattatccg ctccttatga ttttagatgt tttgagataa
    13921 aaaaagtctc aattctttga ccagcaagga attgagaact ttaaaaacat tattttgttt
    13981 cacggtgcaa agtaactttg cgttcgcgtg ggcaatattt ttgtttttcc aaacgatcag
    14041 gattgttacg tttgtttttg cttgttagat agttacgttc tttacaagaa gtacactcta
    14101 aagtgatgtt tacgcgcatg tttttccctc ccatttttac aagaatcctc taagatgact
    14161 ctcagacttg attatcttac catgattttt tctagattgc tagtgttttt cacgattttg
    14221 ttaagtaaaa ctacttgacg ataaatacaa cggttttact tctcaaacaa aatctttttc
    14281 tttttgtata aataccctta cacttgttta tatagattat gttttttaat aaagggggat
    14341 actaatatgc catggaacat gaaggacttt cctgcatcaa tgaaaaactt agataaattg
    14401 acaagaaaaa aagcaatcga catcgcaaat gcattattag atgaagggta ccctgataac
    14461 cgtgcgatcc caattgccat tgatcaagca aaaaaatggt atgaagatgc tagtgaatca
    14521 gaaaaacgaa ctttcgaaaa agaaaaaaat ccaagcaaga cggatgaaca cgatacgaat
    14581 cctcgtgctg gaaaactgct tgattcagat gtgatcgtag aatatgagga agaacaatgg
    14641 atcgtgaaat ctaaaggggc aaaaaaggca agcaaccatt ttgatacaaa aaaagaagca
    14701 atcgaaaaag gaaagcaagt agcacaaaac aaagaatcaa ctttgttcat ttataaaaaa
    14761 gatgggacaa aagaaaaaga aatcgattat tgaacatatg taaaggggtt gtgacgaaaa
    14821 tcgtgtcaca acccccttat gttcgaaaca atggtgttcc tctgccaact ccgaaccata
    14881 agtcataata tctataaaac gggaagagct gttttttgaa attgtttctt atcactcagt
    14941 gttaaacggt aggagaaaac ctctttttta ttgatatttg attttcatca aacagtcaca
    15001 tagtcgattg cttctgcttg cggaatcgtt tcatctacaa agtagacttt ataccatgtc
    15061 aagaaactga agattgtcca gatttttctt tgctcttctt tacgtccttc gtgatggtca
    15121 tctagcaact tcatgatttt ttcttgatca aaaaattctt ttgcaaaatc tgcttcgaat
    15181 aatgctttga cttgttcgta gccgtgttct tctttcaacc aagctttgat tggcacaggg
    15241 aatcctaatt tcacacgatt cgaccattct tccggaagat gtttgttcgc agcttgtctg
    15301 aaaatatctt ttgtgttgtt ttgattcaat aaaaacttac ttggtatttg ttgagccaat
    15361 ttcatgactt cgatatccaa taaaggtact cggacttcca aagaacttgc catagaaagt
    15421 ttatctgcct ttaacaagat atcttttggc atccattgat gaagatcgac atattgcatc
    15481 ttattgactt catctttgat tccttctgtt ttctcgtaat gaacagtcat gatttcatcc
    15541 acagatggtg ctgtttgata ttttggtgtt aacacttctg atgcttctga ttcttcgaac
    15601 actcgagcat gaccgataaa atattctttt gcaggagcaa gtgattcata taaatggatc
    15661 cgtccatgga aatttttcat ttttccgatt ttacgtccta aattatagcg tgttcctttt
    15721 ggtaattttt tcaatccttg agcaatcaca cggatgaatt ttgaattcgt atggaatcca
    15781 tacgtttggt atcccgcaaa cagttcatcc gccccttctc cagattggac tacccgcaca
    15841 ctttgtgaag caagatttgc taaaaagtac aaaggtacgc aagaaggatt tgaatcaggt
    15901 tcatctaagt gatactggat caaagggaat gctttgaacg acatgtctcc gtcgatcact
    15961 gctgctgtat tatctaagtc caatagttcg gttagttttc tggcttcaat cgcttcatta
    16021 taggttttat catcaaaccc gattgagaaa gaatgatctg gtttcaatac agaggttaca
    16081 taactggaat caaccccgct ggataagaaa gaaccaactt ctacatcact gaccgtatgt
    16141 gcttcaatag aagcttgaac ggtttcgtcg attttttcga tccattcttg tcgggaatgc
    16201 gtttcttttg tttcaaaatc tgcatcccaa tattgctgaa tgtctatttt tccatcttta
    16261 tacgtataat aatggccttc aggcaaacga tagacatctt tgaagaatgt ttcgccgttt
    16321 aatggagaat attggaaagt catataaggt ttcaacgctt ctttgttcaa ttctttgtta
    16381 aaatcaggat gaggcagaaa acttttgatt tctgaaccaa acataaatgt tccgttcatt
    16441 tctgcataat aatatggttt gatcccaaag tggtctcttg caccgaataa ctcattcttt
    16501 tcattatccc aaatagcaaa agcaaacatt ccgcggatct tttgcaataa ttctgttccc
    16561 cattcttcgt aaccatgcaa aaggacttct gtatctgcat gtgtttgaaa aacgtggcca
    16621 gctgcaatca attcttctct caaaggctgg taattataga tttcgccatt aaaaataatg
    16681 atctttgtcc gatcttcatt gtaaataggc tgagtaccgc cctctaagtc aataatactt
    16741 aatcttctga atcctaaagc aacatgttta tcgatatatt ctcctgaact attaggtcca
    16801 cggtgaacga ttcgatccat cattgtatta ataattgttt ttttgttgtc cttgtcgtta
    16861 acaaaaccta caataccgca catttaactt tcacatccta atataaaatt actgtttatt
    16921 tcattcatat gcatcttatc atagaataaa tagcggacga atattttctc tcttacaatt
    16981 tcgtcacgtg aaaacaaaag atgaaagttt ctgtttttac agaaaataaa gtcccaatat
    17041 ctaatttacc atgttttctt ttttgaggct accttattct cttcgtgtta aaaaaatatt
    17101 agtggatgct atttccaaaa aaaccagcaa aaaaagacac gacctaaaga tcgtgtctaa
    17161 ttatgactgt atcagaaaat atgataccaa acggatctat tcattaatta attgacatag
    17221 tttatctgtt gctcttttat gactggataa ctgaccaatt catgacgatc caagtcttct
    17281 ttattcatat ttacagcagt aatttgactg tcttcatacg tacgataata ataaatccct
    17341 ttatcaacgt tacagcaaga agaataaatc gtatattcgt attttctttc tccagtatcg
    17401 catactcctt tttgttgctc gactgagcct agtatatgaa aaaattgact gatactttca
    17461 gattctgaat cacctgatgc agcattcatt ttagtaaatg tggcttttac aaatcgagaa
    17521 acagaagaga gatctccagg taagcccaat cctcccatcc cacgactata aacattcaag
    17581 ctgatctggt ttgaaaaatg attttctggt gttccatttg atagcgagcg ataattgttc
    17641 agattaaata attgataatc aaatggcgga ttatttgtca acacaccgac aggattatca
    17701 tataaatgaa gtccatcttt catactttca attactatcg atttttcttt atcagctaat
    17761 aaccagtgta aaggtgacaa gggtagtttt tcactaaaat cgatattgac tagattgata
    17821 gtagccaata attctttagc ttcgtctatg gttgagcatt gccctagtat ccatggaata
    17881 aactcaaatg gtgcaacgtt atcttttcct tcttgtaatt ctttataatc agcattgcca
    17941 ggaaaattca gtcctgccat actcaaccct ttttcatttg tagcctcata atagagtgga
    18001 tagttggata ccccagctgc tattcctatc attgcataat gtttatctaa attttttacc
    18061 tttctaaaat cgaaacgata atttcttgga gtaatagtga ccacttcatt ataagacatc
    18121 tcataatcaa aattccttcc aaaataatgg tctttcgttg cataagtgat tgcggtacac
    18181 atcgtttccc gtcctaaaac aaatcgtttt tacactttta tcttacatct agtatttttt
    18241 ttatcaaaaa aaggccaaag aagattgatt tactagcatt tataaaaaat agtactcagg
    18301 gggaatcaaa aaatattgta ggttaaaagt acaacaataa ggggctgtga caaaagtctt
    18361 gtcacagccc cttattccga acaaacggtg ttcaaaagct tgcatctgcg gtaataagcc
    18421 caatcaaacg aaaaaatagg aaaagctatt tttgtttggc cgttcttatt acttgatgca
    18481 ggacagcttc ttcacaacct ctaatcgttg atataaggaa ggtacaggat ttgaacctgc
    18541 gcgccggtat tagccggttc gccggatttc gagtccggtg cattaccact ctgccaacct
    18601 tccgtaagta ctatttttaa acttttttca cgaaatgtca agcctatttc taatagataa
    18661 ataacagaaa tcgctttttt tagcaaaaaa gtccattgac agtttgcagc caacggactt
    18721 accgatcaaa ttcatactga tagaacacta tctgattatt tcaggattat ctaatgctaa
    18781 aatcgagtca attgtccgtt tgatcagtgc aatgatttgt tcttctgttg gatgcatgat
    18841 gtttgatgac atcaatgacg ctaagccagt cactgctacc catgtaccag tatgcagagc
    18901 atcgacatat tcatccgata attgattgta tctttcatct tctttaacta tttcacggaa
    18961 ataattgtat gaaaaatcat gcattttctt tccgtcccca tgttcatcaa tgtacaatgc
    19021 catgtaaaga ctatgatgct tttttgcaaa gtagatatag ttcaaaccga gatcaatgat
    19081 tttatcacca gtacgttcaa tagggaaaac atcttctttt agttgttcaa agatagaatc
    19141 cagcaacgta ttttttaaat cttccatgtt tttgaattcc agataaatcg gctgtgtcga
    19201 aatccccatc tttttagcaa tatttctcgc agtaaatcct ttaaaccctt cctttgcaac
    19261 aacatcataa gcagctttta gaatttgatc cttggtatag acttttcttc tcattaaaac
    19321 tcccccactt ctttactgca gtatatgttg ttttattgta ccctattttt agcatttttt
    19381 gcaaacattt tactcaaaac gttatatttt aaattttttt tcagtcttct ttaacttttt
    19441 tttgcaaaat taaaaaaatt tggttaaatt caatgggttc
//
LOCUS       DACDEG010000044        19305 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.046, whole genome shotgun sequence.
ACCESSION   DACDEG010000044 DACDEG010000000
VERSION     DACDEG010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.046"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            633..1961
                     /locus_tag="IV142_001603"
     CDS             633..1961
                     /locus_tag="IV142_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP8485267.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2163..2996
                     /locus_tag="IV142_001604"
     CDS             2163..2996
                     /locus_tag="IV142_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP8485268.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            3012..3749
                     /locus_tag="IV142_001605"
     CDS             3012..3749
                     /locus_tag="IV142_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP8485269.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3904..4872)
                     /locus_tag="IV142_001606"
     CDS             complement(3904..4872)
                     /locus_tag="IV142_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP8485270.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELKKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5104..5937
                     /locus_tag="IV142_001607"
     CDS             5104..5937
                     /locus_tag="IV142_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP8485271.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5971..6810
                     /locus_tag="IV142_001608"
     CDS             5971..6810
                     /locus_tag="IV142_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8485272.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6836..7396
                     /locus_tag="IV142_001609"
     CDS             6836..7396
                     /locus_tag="IV142_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8485273.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7595..9316
                     /locus_tag="IV142_001610"
     CDS             7595..9316
                     /locus_tag="IV142_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP8485274.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9481..10623
                     /locus_tag="IV142_001611"
     CDS             9481..10623
                     /locus_tag="IV142_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8485275.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10639..11850
                     /gene="thiI"
                     /locus_tag="IV142_001612"
     CDS             10639..11850
                     /gene="thiI"
                     /locus_tag="IV142_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP8485276.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12423..13070
                     /locus_tag="IV142_001613"
     CDS             12423..13070
                     /locus_tag="IV142_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP8485277.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            13463..16108
                     /locus_tag="IV142_001614"
     CDS             13463..16108
                     /locus_tag="IV142_001614"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP8485278.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16418..17731
                     /locus_tag="IV142_001615"
     CDS             16418..17731
                     /locus_tag="IV142_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP8485279.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17721..18374
                     /locus_tag="IV142_001616"
     CDS             17721..18374
                     /locus_tag="IV142_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8485280.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18429..19100
                     /gene="radC"
                     /locus_tag="IV142_001617"
     CDS             18429..19100
                     /gene="radC"
                     /locus_tag="IV142_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HAP8485281.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGIYKLVHINYIRKFTVILPSGNTIMLSIIERHNAFFSS
                     SEDSL"
BASE COUNT         6477 a         3263 c         4043 g         5522 t
ORIGIN      
        1 atcatgatgt cgtaggttcg agtcctactg ccggagtaaa taaagcttat gcaaagaaaa
       61 agaaatccaa tcacttgggt ttctttttct tttattatta tgatgaatca agctttctgt
      121 ttttgtcacg tatgttgtaa tctttacgaa agagtagagt tggctactca taatcaacca
      181 tacttcccat catcgctaaa tacgaaataa atcagcattc ctacaccaat caaagcacaa
      241 gctgcaccaa gaaaagggat gacattccca aagttatctg tattcataag tatgccacca
      301 cccacaaaca aaaagccgcc agcaatcagg tttttcattt tattcatctc ctttcttacg
      361 ttatagtatt tccttctttc aatgagtatt ttgaaagcta tcttattgta tgatgttctt
      421 ctaggcctct tctttattat acaggccatt ttcgttatct catagaaata ttccttttta
      481 tctgaaaact attgtatacc gtatgacatt tttcttcatg aagttgtaag tatattctca
      541 ttcaaatgaa aatttcatca tagaagagat tgtatttcta aattcttaag agtaaaatac
      601 gaagtataac ttggatttgg aaggatgatt ggatgaaagc tattacgcct gatatgacaa
      661 caaaatttaa caaacagttt caagcaaata tcaaacagca ggcattgcag cgggcagttg
      721 tgaagaacgg aattactgct tctgctgaaa atgtttcagc acatgtggag aatacaccgg
      781 tattttctat agatttggct acggggaaag tggctaatca aatgcaatcg gggcgttgct
      841 ggatgtttgc tgcactgaac acattccgtc ataagatgct gaatcaattc catttaaaag
      901 agtttgaatt atcacaaaat tacacttttt tctgggataa atatgaaaag gcaaattatt
      961 tctatgaaaa catcattgca acaggaaaag aggcaatcga tagccggaaa gtagctttct
     1021 tactcgctac tcctcaacaa gatggcggcc aatgggatat gatcgtttca ttattccaaa
     1081 aatatggcgt tgtgcctaaa tcggtgatgc cggaaagtgc gaatagttca aattctcgag
     1141 atttgaataa ctacttgaac aaattactgc gtaaagatgc agtagtgtta cggaaaatgg
     1201 ttgctcaagg agaaacatta gacgctattg aagaaaagaa agaagaaatg ctagaaagca
     1261 tttataactt cttagctatc tcattaggaa cacctccaaa agaattcgat tttgagtacc
     1321 gcgatgaaga aaagaactat catcttgacc gaggactgac accacaaatt ttttatgata
     1381 aatacatcgg tgtaaaatta gatgattatg ttagcgtgat caatgcacca acaaaagaca
     1441 aaccattcaa tcgttcttat acagtagaga tgctaggaaa tgtcgttggc gggaaagaag
     1501 tcaaatatct gaatgttgat atggaaactt tcaaaaaatt agcgattgct cagcttgaag
     1561 aaggagaatc tgtttggttt ggttgtgatg taggtcaatc ttctactaga actagtggca
     1621 tcatggcctt agatgcttat tcaatggatg atctatttga tacggatttt acaatgacta
     1681 aagctgaacg tttagatttt ggagaaagtc tgatgaccca tgcgatggta ttgaccggtg
     1741 ttgacttagt agacgggcaa tcaacaaaat ggaaagtcga aaatagctgg ggcgaaaaag
     1801 ttggaaagaa tggcttcttt gttatgagtg atgcctggat ggacgaatat acgtaccaaa
     1861 tcgttgtaag aaaagattta ttgacgaaag agcaattaaa tgcgttcaat gaagaaccaa
     1921 ttgttctatc tccgtgggat ccaatgggtg ctttagcata gacgagtatt tcaatgaaga
     1981 gtatgtagta aagataaaaa gagctggctt aagccagctc tttttatctt taaagtccat
     2041 tccctgtttt ttatggtccc tgtgctataa ttctaaataa aggataagga ttggaaataa
     2101 tccttttatc attaaaaata tgatgacgaa gtagaataaa attgggagta ggggggaaag
     2161 tcatggatat ccaaactgtt ttatctgatc tgaaattaaa tggtaaagtg attccagttg
     2221 ttggtggcga tgttaatcag acctatcgta taaaaaccga acatcgtgct tattttttaa
     2281 aaatccatcc gaatgtaaaa aaaggatttt ttgaagcaga agtagacgga ttaaaagaac
     2341 tgtcagcatt cgtacgtgta ccggatacgt atatgctagg tgaaacaagt gaaggcgctt
     2401 atttgttaat ggaatggatc gagccgggaa aaggggatca acgagatctt gctgcagctt
     2461 tagctaatct ccatcagcaa acagcgccac aatttggttt tcgtaaagat aactatttgg
     2521 gtacacttgt tcagaaaaat tcttttgaag aagattggtg gacttttttc tttaaagatc
     2581 ggcttgaatc ccagatttct ttggcagaag aaacgaatcg atggaatgta cagcgtcaag
     2641 aaaagtatct gcgatttaaa gaacgtgtat tgaaatcggt tgaaccaaaa aagatcactc
     2701 cacgcctctt gcacggagac ctgtggagtg gtaatgtgtt ctttgaccag caagggcatc
     2761 cagtttttgt cgacccagct gtttcttatg ggaatcggga acaagatatt gcaatgagcc
     2821 agttattcgg tggtttccga ccggaatttt tagatgctta tcaaactata tttccattag
     2881 aaaagggatg gaaagaccga ctgccgattt accagttata ttatcttttg gcacatttga
     2941 atatgtttgg agaaagttac ggttcccaag tagatcaatt actagaaaat ttttaagagg
     3001 agggcgtgtt tttggcaaaa aaatatacca aaaagcatga agtttcatat tatgagtgtg
     3061 acatcaacca aacgatgaca tttccttcga tgttaggagt agttatcaaa acgtctgaag
     3121 atcaaagcga ccagttggga agaggcagtg aattcgtcaa tagctttgga cttacttggg
     3181 tcatcacgaa ttattccatg gagattaatc gtctccctag agtcggtgaa gtaatctcag
     3241 taacgactca agccatggag tacaataagt ttttctgtta tcggaacttc tggatccatg
     3301 atgaagatgg gaaagaatta gtcaaaatag aatctgtttt cgtattgatg gattttgtga
     3361 atcgaaaaat gagcagtgtt aatgaagaga tcattgctcc ttttgagagt gagaagatca
     3421 agaaaatcaa gcgtcaggaa aaaattgaaa agattgattc gggcttcatg ttgccatatc
     3481 gtgttcgttt ttatgatatc gatagtaatc agcatgttaa taatgcgatg tattttaatt
     3541 ggatcattga cgtattagga tatgactttt tgactacgca tgttccagaa aaagtaatta
     3601 ttcgatttga taaagaagta gagtacggga atgagatcga aagccatttt gaacaaatcc
     3661 ctatcgaaaa tggtgtcaaa acaaaacatg aaatccgaat gcaagaacag ctgtattgtg
     3721 aagcaaatat tatatggaaa aataaataaa aaagaggttg tgacagaagg gggcaacttc
     3781 aagaaataag aagaaattta cgaaaattgt ttctcaaatt tttgtaaatt ttcgacttag
     3841 ttttcgaaga agctattttt gtcacagcct ctctttttaa gtttagctat tagataatga
     3901 tgtttagttt tggataaacg aaatcaattc ttcacccgac aaaccatagt ttacagcaat
     3961 caatagtttt aaacgtgctt tttgactatt aatactgtta cagaaaataa tccccatttt
     4021 ttttagttct tttcccccgc cttcataatc ataaacaggc tctgcgattc cattgaaaca
     4081 acgagaaaca agtactacag ggattttttg gacaagaagc ttttccaaag cagctaaggc
     4141 ttgtggaggg agattccccg cacctaaagc ttcgattacc aaaccgtcta ctttcgtatg
     4201 ggcgatggct tccaataagt ccccttgcat cccagcatat gctttaacga tcgggatagt
     4261 cccatctaca gcggaaatgt ccagtcgctt tgtttctaaa agttcctgca agaataaaat
     4321 tcgattctta gttactaagc cgatgggacc caatgtaggt gttcggaaag tagcaacatt
     4381 tgtggtatgg gtctttgtca cgtagcgtgc agaatggatc tcatcgttca tgaccaccaa
     4441 aaccccctta tctagcgctt cttcacaact tgcgacacga atcgcacttt caaaattata
     4501 aaggccgtca cttccaagtt cattacttga acgcattgcc ccagtcaaaa caattggtaa
     4561 tttgttgcca atcgttgtat caagaaaaaa agcagtttct tctaatgtat ctgtaccgtg
     4621 tgtgatcacc actccggata tacctgatgc ataagcggac aagatacgtt cttttaatac
     4681 caacatgtga gaaggcatga tgtgtggtga gggaagattg aagatatctt caactaataa
     4741 ttctacatgc ttggggattg tgatttctgc atctaataat gggtttgcca catcaggact
     4801 aacaccgccc gtgagcatgt tttctttcat ggcgatcgtt ccgccggtgt gtagtacaag
     4861 tatttttttc attttacaga cttccttacg aataattttg catagccgtt aaatcttatt
     4921 gtatactata taaaagagtt ttgctatcag tgaagaataa cgaatagcct aacttatcat
     4981 aaaaaagctg accttattaa aatggaaaag actcaacctt gacctcctaa tacaatggag
     5041 tatctggttg agtttctttt acaagaaagc agccaaggaa atcaatcacg aaggtggccg
     5101 gaaatgatca gaatcggatt aacatcgttt aaagaacatg acaaattaac tggtaaaaag
     5161 aataacactc tttatgaata tgcagggttt ctacccttag tggaattaga tactgcctat
     5221 tatggaattc cccgaaagtc ctcagttgag aattggataa gtgaagtacc tgaatcattt
     5281 cgctttgtag tcaaagtcta tcaaggaatc agcggtcaag gagactggaa gccttattat
     5341 cagaccgaag aagagatgat tgcgcatttt ttagatgtta tgtcgccaat ggtagatgct
     5401 ggaaagatgt tttgcttttt agtccaattc ccctcatcat tcaaatgtac gaaagaaaat
     5461 gtccagtact tgcgcaaact tagaaagtgg tttgagggtt atcctgtttc tatagaattg
     5521 cgggattatt cttggtacag cgatgaattt cttgaacgta caagaaactt tatgtctaga
     5581 gccgcttttt ctttagccat catcgatgag ccacagttgc tgaatacaac gattcctttt
     5641 gatccatttg tcaccaatcc gaactttgct ctttttcgat tccatggtag gaatgcgcaa
     5701 ggatggcaag caaatgataa agagtggcgt aaaaaaagaa cgttatatcg ttattcagaa
     5761 aaagaactgc aagaactagc acatgaagtc aaagtagttg attctaagac aaaagaggta
     5821 ggtgtgattt ttaataataa ttctggtggt gatgcggctg aaaatgcatt agcactgcaa
     5881 aaaatattaa atatcaagcc agatggactc aatccaaccc aaatggatct gttttaaggg
     5941 cttattttat tcaagaaagg ggcgaagtct atgatcaaag ctattttttt tgatattgac
     6001 ggaacattgg tcaccaatcg ttcaaaagcg ttggaatcaa caaaacaagc aattgaatat
     6061 gctcggaaaa acggcatttt atgcggtgta gcaacggggc gcagcccagt aaagatcaaa
     6121 gaaatcattg atgaattgga attagatatg tatgtcgtgt ataatggtca actggttttt
     6181 actgcagatc gcacgattat cgaccaccct tttgaacaaa aagtattgga acatattgtt
     6241 gaatttgcag atgagaacca tcgacaaatc gtttttggtg cgagaaatcg gttagatgga
     6301 tcaacgacga tgctattagg acaatcgatt tttatcaagc gactagtcag ctttttaccg
     6361 cgtaaattcc cggtacgtct gatgaaaaaa atcctccagg tttttagccc tcaccgacaa
     6421 aaggatcgtt atgaaacgtt agagatatta aaagaaccaa tttatcaatg tatattgctc
     6481 agtccggagt cagaacagaa aaagttagaa catcgctttc ccgagtgtac gtttcagcgt
     6541 tcaaatacgt atactgtaga tatcatccca aaaggtggtt caaaattact aggcatccaa
     6601 gctttcgcaa atgctgtcga aattgaaatg gaagaaatta tggcattcgg cgatcattac
     6661 aatgatatcg agatgctgaa aggtgtagga attggagtgg ccatggggaa tgctcagatc
     6721 gaagtaaaac atgctgcaga ctttgtgact caatcaaatg aagaagatgg gatatacgaa
     6781 ggactgaaac acttcggtct gatttattag tcaatgaaag aaggaaaaaa tcagtatgga
     6841 agcagagaca ccttttcaaa aagtagaaac attccacaaa acttttgatc caagaagacc
     6901 agagataccc acggctttta cagaaaatga agctcataca cgtgctgcgt tcaaaataga
     6961 agaacttgtt gaatttgttg cagctacttc acctaataaa gaacaactgc atgaagctgt
     7021 tatgctgctg cacgaagcat tagataaagc agaagaaaaa atacttggta aaacacagtg
     7081 gggaagtgat ccgctggttg aacaagtaga tgcactgaca gatatccttt actttactta
     7141 tggttctttt tcattactag gaatagtgcc tgatcgaata ttcgaaatcg tccatcaggc
     7201 aaatatgggg aaattatttc cagatggaaa acctcattat gaccctgtaa caaataaagt
     7261 gttgaaacct gaaaactggc agatggaata tgctccagaa cctaaaatcg cggcagaaat
     7321 ccaacgtcag attcaagaag cgattgctaa gaagaaatcc gctgatatag gcatgtgcac
     7381 aaatgaaaaa gagtgatcag agtttcacta tttttgttat ctttatacga tggaaaaaca
     7441 aaagattttc gtttaagtta gtgaatgcat gaaaatagta tgctataatc tagaatagat
     7501 gtcttttgca cgagagacgt attatttgga tttatgccaa gcagaacgcc atctgttgca
     7561 taaaagatgc atactgaaaa cggaggaacc ataaatggga aataatgtta tcatcggaat
     7621 cgtcattgca atcattatag tcgccattgt cgtctttttg gttagttact tgatgaagaa
     7681 aaaaaaccaa gatcgattgg atgccttaga aaaacgcaag gaagaattat ttgacttgcc
     7741 agtgattgaa gaagtcgatg atgttaaaaa aatgcatctg gttgggcaaa gccaaaatac
     7801 ctttcgtgaa tggagtcaaa aatggaatga catatctact tcctcgttcg ctgaactaga
     7861 aagtcaaatt ttcgaagtgg aaaacctcaa tgaaagtttt cggttcttaa aaggaaaaaa
     7921 agcagttgtt gaagcagaag ctacaatgga ccaaatggag aaacaagtga cggaaatccg
     7981 tgaaggtcta aaggaattgc gggaaacgga agaacgcaac tcactagctg ttcagcaggc
     8041 acttgacgtc tatgaagaac tgaaagaaaa agtcagagaa gacgagcatg aatacggacc
     8101 agctcttttt gaattgcaaa aacagatcaa agtgatcgaa aaagaattta cgcagtttgt
     8161 gactttaaac acttctggag accctgttga agcccgcgag gttttggata aagcagaaaa
     8221 acatacgtat gaagtggaag ctctgatgaa aaaaattcca ccgctgtatg aggagctcca
     8281 taccactttc cctgaacaac ttgaagaaat cagtgatact tacgagaaat tgatggaaga
     8341 acaatatgta ttcccagaag aagatcttgc tgaagatatc gcaaaagtaa gtcgaagaat
     8401 tgaaaattca ttagccaatc ttgaaaaaac agaagttgaa acggtcgaat ttgaaaaccg
     8461 tgaaacagct gatttgattg attcgttata tgatatctta gaacgggaaa tggaagctca
     8521 gagatatgta aaaactaacc aatcaacgat tgcagagtat atcaaacata caacaaagaa
     8581 taaccgtcaa ttattgattg agctggatca cacatctcaa agctatacat tgaaccacaa
     8641 tgaaattggc cgtgtacgag gtttccaaac cgaagtggaa gaaatggaac gtcaaaatga
     8701 acagatgatt ccacagatcc gtcaacacga aattccttat tcagaagtgc gcacgttcta
     8761 taaaacagtc ttcaaagtct tagaagatat cgaaaagcaa caagtagaga tcgacgactc
     8821 attacatgaa ttgagaaaag gcgaaaaaga agcgcaagaa aagatcgata cattcgaatt
     8881 caaattgcgc agtttgaaac gttttgtaga aaaacaacgt ttgcctggct tgccgaatga
     8941 ttacctagaa ttcttttttg ttgctactga ccgtattgaa gaactaagtg ttgtcttgaa
     9001 taaaattcga gtaaacatgg aagaggtcaa tcgtttagtt gctctatgtg aagaagatct
     9061 agaattatta gataagaaga cacatgattt agtagatgca gcagcattaa cggagcaaat
     9121 gctccaatat gcaaatcgtt atcgtcatac acacgaagaa gtgagagaag caattgataa
     9181 aagctactat ttattcaata aagaatatca ctatcaagaa gcattagacg aaatcggtac
     9241 agcattagag cgagtagaac ctggtgcttt caaacgcatc gaagattttt attttaatca
     9301 tccagactta gtataaagca aaaaagttag taaaccagca agagcaagac ggattattgt
     9361 cttgttcttg tctttttttg tatatttgaa aattgaaaca agtgaaaatt tcacctgctc
     9421 ttgaaagcac cttggacaac cgttataata gaaaagaatt tcaaaaaaga ggggtttctt
     9481 atgatatatt ttgataacag tgcaacaacc ccaatctttc cgcaaagctt agatgcatat
     9541 gtaaaaacca gtcagcggat tattgggaat ccatcaagct tgcatgattt aggcaatcaa
     9601 gctaaccggt tgttgcagca agcaagaaag cagatcgctg atttgatcca tgtagaacca
     9661 gaagaaatct attttacttc aggcggaaca gaaggagata actgggtatt aaaaggaacg
     9721 atgatcgaaa aaagagaata tgggaatcat atgatcattt caggagtcga acatccggcc
     9781 gtcagcgaga cagcagaaca attaaaagaa ttaggaatcg aagtaagtat cgccccagtg
     9841 gataaacgcg gatttgtacg tgtcgacgaa ttgaaagaat taataagaaa agagacagta
     9901 ttagtctctg ttatggctgt gaataatgaa gtaggatcga ttcagcccat ccaggaaatc
     9961 agtgatcttt tggatgattt tcctaaaatt catttccatg tagatgctgt tcaggcgatt
    10021 ggaaaagtac cattctcgca atggttgaca gaaagagttg attttgcaac attttcagct
    10081 cataaattcc atggcccacg gggaactggt ttcatttatt ggaaaaaggg tcgtcgttta
    10141 gcaccattac tgacaggtgg agggcaagaa agaaatcagc gaagtgggac agaaaatgtt
    10201 ccaggtattg tggctatggt taaagcttta cgtttatcat tagagataaa ggaacaaaaa
    10261 cctgagcaga cacaacgctt aaaagtagct ctaatcaatg cgttgaagac gtatgagaaa
    10321 gtgacgattt tttcagaagg aactgactat gcaccgcata ttttatgttt tgctctaaaa
    10381 ggaattcgcg gcgaagttct cgtccatgcc ttagaagaaa aacaaatatt tgtttctact
    10441 actagtgcct gctcctcacg taaacatatg gcaagcagta cattgcacgc aatgcatgta
    10501 ccaaatgatc tggctacatc tgctgttcga atcagcttag atgaaaacaa ttcgcttgca
    10561 gaagtcgagc aatttatgat tattttcaag cagctttata agaaattttc tgcaatcaat
    10621 taaaagagga gtgttcttgt gaaatacagt gaaattatgg tccgttatgg ggaactttcg
    10681 acaaaaggaa agaatcgaaa aagctttatc atgcaactag cacaaaatgt cagacaagct
    10741 ttgaaagact ttccagaaat caaaatccat gctgatcggg accggatgca tcttttattg
    10801 aatggcgcag acgggcagct agttattcca aaactagcaa aaatatttgg gatccaaaat
    10861 ttctctccaa gtattcgtgt agaaaaagat gtcgaagttt tgaaacaagc tgttcaagac
    10921 atcatgaaag aaatttatac tgggaaagaa acatttaaaa ttatcgctaa aagaagcgat
    10981 caccaatttg aattgacaag taatgaattg aatcaaacat taggtaatgc tgtttttgat
    11041 attttcccgc atatccaagt tcaaatgcgt caaccagata ttccattacg tgtagagatt
    11101 cggcgtgatg gcgcttatct atcgtatgaa acgatcaagg gagctggagg attgcccgta
    11161 ggaacaagcg gaaaaggaat gcttatgctt tctgggggga ttgattctcc tgtagcaggg
    11221 tactttgcca tgaagcgggg agtggaaatt gaagctgttc attttgctag tccgccgtat
    11281 acaagtgaac aagcactgca aaaagcaaaa gatctaacag ccaaactagc accttatgtg
    11341 ggaacgatcc attttatcga agtgccattt accgagattc aagaagaaat caaaaagtca
    11401 gtgccacaag gatattggat gacaattact cgtcgaatga tgttgcgttt gaccgatgaa
    11461 attcgccgta ttcgtcatgg tttagtcatt ttgaatggtg aatcattagg tcaagtggct
    11521 tcacaaactt tgcaaagtat ggtggctatc aatgaagtaa cgaatacgcc aatcattcgg
    11581 cctgtagcga caatggataa acttgaaatc attgaagtag cagaacagat cgatacattt
    11641 aatctttcta tccagccttt tgaagattgc tgtacgattt ttgctcctcc acagccaaaa
    11701 acacgccctc aactggaaaa ggtccatcaa tatgaagagc gtttagcaat agatgaaatg
    11761 atccaacgag cactagctgg cttgaagatg gaagagatac aagcagaatc gtctaatgaa
    11821 aaaactgaag aattttcaga attgctataa gaggttatga aaatagtgtt ttgaccagag
    11881 tattagagaa acaaaacgaa aaaatagctc catgtatttt accgtttttg aactaatgtc
    11941 gaagattagc tgattgaata ccatttatca agaagttatg gaaaagccgg ttctttttta
    12001 ctaactgaaa aataaaattt tatttagaga tcaagctaca gttgataaat gatataaaaa
    12061 agagaatttc ccatgaaata gcgaggttgt gacagaagtg ggcagcttca agaaataaga
    12121 aggaatttac gaaaattgct tttcaaattt ttgtgaaatt tcggcttatt tttcgaagaa
    12181 gttacttctg gaacaccgtg tatcagaggt tgcgaaaaag ctgccctgca tcaagtaata
    12241 agaacagcca aacaaaaata gttcctcata ttttacgttt ggttgagctt attaccgcag
    12301 atgcaagctt ttgaacaccg ttttatttgt ctcacaccct tttccttcct acttgtaacc
    12361 ttgctcccgt gttagaaaca tgttacacta aatatgtgaa attattaaca aggagcgtcg
    12421 ttatgcgaga aaaaacagat acactgccaa aagcaactgc gaaaagatta cctttatatt
    12481 tacgctactt gaagatgctg gatgactcag gtataagtag aatcaaatca aatgagttca
    12541 gcgaaatcac ccaaattcct tctgcaacga tccgcagaga cttctcgcaa ttaggcgagc
    12601 taggcagaag cggatatggc tatgatgtcc cttttcttat cgatgtattc aataatatcc
    12661 ttaatactaa agaagaaaaa cgaatcgcat tagtaggata tggaaatctt ggcaaagcat
    12721 tgaaacacaa caattttaga agaaatgaaa atctaaatat cgtgtgcgtc tttgataatg
    12781 atccagcact gatcaatcga gtaattgatg gagaaatgat ctatccgatt gatcgtttcg
    12841 cggagatagc caaagcaaaa aatgtgacag tagcaatctc tacggtacca agtaaatatt
    12901 cacaatctgc tattgatgaa attgtcaaag gaaatgtaac cgctatactt aattttgcgc
    12961 ctgacagagt aactgtccca gcttatgtca atgttcaata cattgatttg acaaccgagt
    13021 tgcagacgct tatttacttt gatgagaatt actcagaagt tttttcttag gaaaatttga
    13081 gtaggcttat tgatcttgcg agaactcgtc gtaattcttt caaaaatttt ttagtactaa
    13141 atgaagtggt tttttcacga ccacttgttg actaaaaaaa aaaaccattg tagaatgaag
    13201 cataacgctg aacaagagga gtaactggca cattcattct tagagagaga actatctgct
    13261 gaaaggttct caatgacaac cagtgaaggt agcttgggag tttctgtctt gaatggagta
    13321 gagatagacg agtcgtgatc gttaactcac ttgagaggca ataatgattg attattgcaa
    13381 tttaggtggt accgcgtaaa tcatacgtcc tagaatacga aagtatttta ggacgttttt
    13441 tagtttaaag gaggaaaaca gcatgacgga agaaaataac ctatcaacaa aatataatcc
    13501 gcaagaagtt gaagctggac gttatcagca ttggttagaa caagatttgt ttaaaccgag
    13561 cggagataaa aaagcaaaac cttattcaat cgtaatcccg cccccaaatg ttactggaaa
    13621 acttcattta gggcatgctt gggatacgac cttgcaagat atgctgattc gtcaaaagag
    13681 aatgcaagga tatgatactt tatggctgcc tggtatggac catgcaggaa tcgcgacaca
    13741 agcaaaagta gaagcgaagc ttagagagca agggatttct cgttatgatc taggtcgtga
    13801 aaagttcatc gatcaagtgt gggaatggaa agaagagtat gcgagccaca tccgcgaaca
    13861 gtgggctaaa atgggcttat ctttagatta cagtagggaa cgttttacat tagatgatgg
    13921 attatctgaa gcagtacgta aagtttttgt taccttatac gaaaaaggtt tgatttatcg
    13981 tggtgaatat atcatcaact gggatcctca agcacgaaca gctctatctg atatcgaagt
    14041 tattcataaa gatatcgaag gcgcctttta tcatatgagc tatgaactag cagatggatc
    14101 aggcgtagta gaaattgcga caacccgtcc agaaacgatg ttgggagata cggcgattgc
    14161 tgttcatcca gaagacgagc gttatcaagc gctcatcggc aaaaaagtga ttctaccttt
    14221 agtcaataaa caaatcccaa tcattgcaga tgaatatgta gatatggaat tcgggacagg
    14281 tgtagtaaaa atcactccag cacatgatcc aaacgacttt gaggtaggta atcgtcatga
    14341 tcttccacgc gtaaatgtta tggatgatga tggtacgatg aatgaactag caggaaaata
    14401 tgctggaatg gaacgttttg ctgctcgtaa agcaatcgtg caagatctga aagaaatcgg
    14461 tcgattgatt aaaatcgata cgatgatcca cagtgtgggt cattccgagc ggacaggtgt
    14521 cgttgttgaa cctcggctgt ctactcaatg gtttgtaaaa atggcgccgt tagcgaaaaa
    14581 agccattgat aaccaagaaa cagatcaagc agtggaattc ttccctccaa gattcaatca
    14641 aacgttttta cgttggatgg aaaatgtcca tgattgggta atctcacgac aattatggtg
    14701 gggacatcaa attcctgcat ggtatcacaa agaaacaggt gaaatgtatg tagggatgga
    14761 agcaccagcg gatagtgaaa actgggtaca agatcctgat gttttagaca cttggtttag
    14821 ttcagctttg tggcctttct caacaatggg ctggccagat acggaaagcg aagattataa
    14881 gcgatacttc cctactagca ctttagtaac cggttatgac atcattttct tctgggtaag
    14941 cagaatgatt ttccaaagct tggaatttac tggcgaacgt ccatttgaga atgtcttgat
    15001 tcatggattg attcgtgacg aacaagggcg gaagatgagt aaatcattag gtaatggaat
    15061 cgatccgatg gaagtaatcg aaaagtatgg agcagatgct ctccgctggt tcttatcgaa
    15121 tggttctgca ccaggacaag atgtccgctt cagctatgaa aaaatggatg cttcttggaa
    15181 ctttatcaac aaaatctgga atgcttcacg tttcgttatc atgaatatcg aaggaatgac
    15241 cgtagaagag atcgatttat ctggagaaaa atcagtggct gaccggtgga tccttactcg
    15301 tttgaatgaa acgatcgaac gagtaactga gctatttgat cgttttgaat ttggtgaagc
    15361 aggtcgacaa ctgtataact ttatctggga tgatttttgt gactggtaca tcgaaatgag
    15421 taaagaagta ctttatggag atgatgctgt ttcaaaacaa accacccgta gcatcttagt
    15481 ccatacactt gatcagatcc tacgtttatt gcatccaatc atgccattcg ttacagaaga
    15541 aatatgggaa catattcctc atcagggaaa ttctctagta gtggcagaat atcctgttgt
    15601 acgtccagag tttgatgatg aaacagcttc taaagggatg gaagtattga aagaattgat
    15661 tcgagccgtt cgaaatattc gttcagaagt gaatacacca ttgtctaaac caattacgtt
    15721 gctaattaaa acaaacgatg ctaaaatcga tcaattttta gttgaaaata caagctatat
    15781 cgaacgcttc tgtaatcccg aagagttgac gatatcaagt gagatcactg cgccagatct
    15841 agcgatgtca gctgttctga caggtgcaga aatcttcttg ccgttagcag gattgatcaa
    15901 tattgaagaa gaaatcaaac gattagaaaa agaactggaa aagtggacaa gcgaagtaaa
    15961 acgtgtccaa ggaaaacttt ctaatgaacg ttttgtttct aacgcgccgg aagaagtagt
    16021 agaggcagag cgtgcaaaag aaaaagatta tttagagaag caaacggctg tcaaagaacg
    16081 aatcgaaagt ttgcgtacta ttcaataaga atagaaaaag agagtgtgaa atcagctagc
    16141 tattactaaa agctagctga ttgaacatcg ttacaggaaa gagtttgagc ctcactctta
    16201 gcttcgattc ataaggagat gtgacataaa ttttgtcgca tctctttttt agtctactct
    16261 tattatgtag gagaaggagg gaggtggatc atgtatcaag agtcaataaa gtagttgata
    16321 aaatcgttcc tctctctgtt tcttttctct ttgtcatact tttatttttc ttatgtgttc
    16381 tatttgttat aataaaagag aaatggggga attgttgatg acgattgaag aagcaataga
    16441 atggatccat agtcgattac catttggttc acgtcctggc cttgaccgag tcgaagcttt
    16501 attagaagtg gtagggcatc cagagagaaa agtaaaaacg atccatatcg ctggaacaaa
    16561 cggaaaaggt tctactgtag cttatttgag aagcctatta gaagaggctg ggctgacagt
    16621 aggtacattt acttcacctt atatcgaaac gttcaatgaa cggatcgcaa tcaatggcaa
    16681 aagcattcca gacaatgatt tgatccagct tgtaaaaaaa tatcagccac tgatcaaaca
    16741 acttgatcaa tttgaagcag tagctggtat tactgaattc gagacattaa cagcaatggc
    16801 atttgattat tttttgatga agaaagtgga cgtagcgatt atcgaagttg gcttaggcgg
    16861 cttattggat tctaccaatg tagctgtacc aatgctgacg ggaataacaa cgattggact
    16921 tgatcatacc gaaatacttg gagatacaat tgaagaaatc gctgcacaaa aagctggaat
    16981 catcaaacaa ggcgttccag tagtaacagg aaatattgcc ccagaagctt ttactgtcat
    17041 ccaacaaaca gctaaagcaa aaaatgcacc gctttatgta tacggggaaa attataaaat
    17101 aaactatctg catcctgatg agatgtgggg agaagtattt gaatttttta atgaaatagg
    17161 aaagatccct caattaacca ctccgctttt agggcaacat caagctgaaa atgcaggagt
    17221 agccatcgaa ttgtattttg tttactgtca attggaaaag ctgcctttcc gagaaaaaga
    17281 tgttttgaag ggattgaaga aatctcgatg gccaggaaga atggagcgtt tgagtaacga
    17341 gccattagtt gttttagatg gtgcccataa tacgcatgca atgaaccggc ttgttgagaa
    17401 tttgaaaaaa gaattcaaag gatatcaaat caacattttg ttttccgcct tacgaactaa
    17461 aaatgttaga gaaatgctag aacgattgca gcaagtacca aatgtccata tttatttgac
    17521 gacctttgaa catccaaaag ccatcgaatt aagtgaagta tcagatttag caagtgagag
    17581 agtatcaatc gtttcattgt ggcaattcgg attagcagat atattggaaa agatgacaaa
    17641 tgaagacgta ttagttgtga ccggttcact ttatttcatt tcagaagttc gccatttatt
    17701 aatacaatta ggaggagatc atgaagtata atgcagtgat ttttgacatg gatggtttgc
    17761 tgttcgacac agagttagtg tattacgaag ccacacaagt agtagcagac aagatgggtt
    17821 ttccttatga taaagacctg tatttaaaat tcttaggtgt ttcagatgaa gaagtatggg
    17881 cgaattatca tatgattttc tcggaatttg gcaaaacaag tgtacaaaga ttcatagatg
    17941 attcatacca ggaaacgctt gaacgttttt cgcaaggagc ggtacaaatg aagcccggtg
    18001 tgttggaact cctctctttt ttagaagaac atcagattcc caaagttgtc gcttccagta
    18061 accaacgagc agtcattgaa cttttattga agaagaacaa tctgtttgaa cgatttgaga
    18121 cgattgtttc tgcagaaaat gttcaacgag caaaaccaga cccagaaatt ttcttgttag
    18181 ctaatcagaa gctaggtaca gcgaagaaag aaacattgat tttagaagat tcacaaaatg
    18241 gtattttagc agcttctgcg gctgagatcc cggtagtcat ggtaccagat ctgcttccac
    18301 catcccaaga attgatcaaa aaaacagctg gggttgtttc ttctttacat gaaatccctg
    18361 cattgttaaa ataaaaatcc atctcttttc gtatcttatt ttagataaga tagaaaggag
    18421 atgttttttt gggaaaacga ttgatcaaag aagtacctac tagctcttta ccaagggaac
    18481 gtatggaaat ttatggcgcg gaagctttat cggatcagga gcttttggca atcttactgc
    18541 gtaccggtca acacccctat agtgtgatgt cgattgctgg aaatctgtta aaaacgtttg
    18601 gaggactggc ctctttacga caggctactt tgcatgagtt agaagagatt cgagggatag
    18661 gacgcgtgaa agcaatcgaa atcaaagcct tgatcgaatt aggtcgacga attcagacgg
    18721 atcatgaaag agcggctcca ccggttcgta ccagctacga attggcccag caattgattc
    18781 tggaaatgaa agatcacaaa caagaacatt tggtttgtat ttatttggac acgaagaatc
    18841 aagtgatctt aaaaaaaaca gtgttcatcg gttcgctgaa tcagtcagtc gctcatccta
    18901 gagaaatttt ccattatgct gtacgttatt gtgcagcaag gatcgtttta gcgcataatc
    18961 atccttccgg aatatataag ttagtacaca taaattatat aaggaaattt actgttatat
    19021 tgccatctgg aaatactatt atgttatcga taattgaacg ccataatgca tttttttctt
    19081 cttccgaaga ttctttataa atttcatcga attcttttga tagaataaat tcgacatttt
    19141 ttatatctat tttagctggc tctacttcaa tttcatttaa ttctttcatt aaagcttctc
    19201 gatctttttt gtattcctct aagtctataa cttcattcaa gtaggctttc ttcaatctat
    19261 ctaatttatt ctcaatattt ttcttactat ttttcttttt aataa
//
LOCUS       DACDEG010000045        18564 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.047, whole genome shotgun sequence.
ACCESSION   DACDEG010000045 DACDEG010000000
VERSION     DACDEG010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.047"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(111..566)
                     /locus_tag="IV142_001618"
     CDS             complement(111..566)
                     /locus_tag="IV142_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485282.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(720..1928)
                     /locus_tag="IV142_001619"
     CDS             complement(720..1928)
                     /locus_tag="IV142_001619"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP8485283.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2040..2372)
                     /locus_tag="IV142_001620"
     CDS             complement(2040..2372)
                     /locus_tag="IV142_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP8485284.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2520..3395
                     /locus_tag="IV142_001621"
     CDS             2520..3395
                     /locus_tag="IV142_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8485285.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3477..4058)
                     /locus_tag="IV142_001622"
     CDS             complement(3477..4058)
                     /locus_tag="IV142_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP8485286.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4064..5323)
                     /locus_tag="IV142_001623"
     CDS             complement(4064..5323)
                     /locus_tag="IV142_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP8485287.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5325..6371)
                     /locus_tag="IV142_001624"
     CDS             complement(5325..6371)
                     /locus_tag="IV142_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485288.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6606..6881)
                     /locus_tag="IV142_001625"
     CDS             complement(6606..6881)
                     /locus_tag="IV142_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP8485289.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7124..8434)
                     /locus_tag="IV142_001626"
     CDS             complement(7124..8434)
                     /locus_tag="IV142_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP8485290.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="IV142_001627"
     CDS             complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="IV142_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP8485291.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9980..10660)
                     /locus_tag="IV142_001628"
     CDS             complement(9980..10660)
                     /locus_tag="IV142_001628"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP8485292.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10709..11344)
                     /locus_tag="IV142_001629"
     CDS             complement(10709..11344)
                     /locus_tag="IV142_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8485293.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11410..12813)
                     /locus_tag="IV142_001630"
     CDS             complement(11410..12813)
                     /locus_tag="IV142_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP8485294.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12800..13831)
                     /locus_tag="IV142_001631"
     CDS             complement(12800..13831)
                     /locus_tag="IV142_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485295.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13862..14083
                     /locus_tag="IV142_001632"
     CDS             13862..14083
                     /locus_tag="IV142_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP8485296.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14248..14985)
                     /locus_tag="IV142_001633"
     CDS             complement(14248..14985)
                     /locus_tag="IV142_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP8485297.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15138..16478)
                     /locus_tag="IV142_001634"
     CDS             complement(15138..16478)
                     /locus_tag="IV142_001634"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP8485298.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16715..18238)
                     /locus_tag="IV142_001635"
     CDS             complement(16715..18238)
                     /locus_tag="IV142_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP8485299.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
BASE COUNT         5247 a         3944 c         3124 g         6249 t
ORIGIN      
        1 aaaaatcttg tcacagtccc ttattctgaa taaacggtgt ggaagagaca gcctatcagt
       61 agtaataaga cacatctctt tcacagcctc atttttgcgt aaaaatcgat ttattcgaaa
      121 aattccccgt atacatcttc tacacgttga tctggattga tcacgatata caagttacct
      181 gttttttcgc tgatttttgt tgattgatat tgattgtcta atccttctgt tcctttatct
      241 agatatggga agtacacaag atcaggtgtg ccatttcggt aaaggatttc cattcgctcg
      301 atatcttcgt atttcaatgc gcgattaaac atgcccattt ccagtccgcc taggttgata
      361 tcatgtgtcg caactgcgtc cccttcatgg aagatttcga ttttaaatcc agcacacgga
      421 tggatttcaa caaattcact gccattgatt cgtccaaaac ttgttgtgat ttgtttgatc
      481 caaagatcgc cgatatgacg gcggttgatc gtccaggttt ctccatttct aaaataaaat
      541 tttagcgctg tcatttcttt actcattaaa aagactcctt tatgatgtaa tttattcttg
      601 tactacctca gtttagcaca aatttatgta agtggtcaca tcttatgttt tttcttctaa
      661 tgacgtatct gcccacttta agtagccaac aaagctgagc agatacagtc tagtgttttt
      721 taaatcaatt tttctttcga taaaaaatcc aaaacctgtt ttttatcatt ttttattctt
      781 tgttctacga cggctttttc agctaattgc aaagcttcac taacccactt accaggtttt
      841 tttccagttt cttctagcaa gattttacca tctactgcta attcgcttaa tgacttgata
      901 ggtaaacgat cataggattc tcctacggca tccatctgag gtgtttcgtc aaaatagatc
      961 atcaattctt ctacttgcag tgcatgtttt ttgcctaaga agtacaattc gaatggttcc
     1021 aaagctccag tttctcgttg tcgtagtcct tggacaagtg cttgaacgtc tttaatcatt
     1081 tggttagaac acttccacga tttcaaaaag gaacgaatct cattttcttt tagatccagt
     1141 tggctgatca ataatgccca tgcttggctc tcttctttca atgggacgga tggtaattca
     1201 gcaaaacgta aaagtgcttc gccttcttct ttcaaaccgg gacagcaaat gtagcactct
     1261 gtttcaataa atgcatggat tcccgcggcc cgttcatttc ccaagagcat cttgatgaat
     1321 tccacattta tacgttctac agatatttta cctaatagct cgtggttttc tttgatggcg
     1381 tagaaggttt cttcctctaa tttgaaccct agttgactga caaaacgcaa cccgcgcatc
     1441 attcgtaagg catcttcatg gaaccgctca tgaggattgc ctacagctcg caagatctgt
     1501 tcttctagat cttgcagacc atcgaacaaa tcgatgattt ctccatcttc tttcaaagca
     1561 aacgcattga tcgtaaaatc tcttcttttc aaatcttctg ccaacgaacg aacgaattcg
     1621 acatgatctg gtctacggta gtcctgataa gttgattccg tccgaaatgt ggtgatctca
     1681 tattgttcat cattttctaa taccaacaca gttccatgtt cgatcccaat atctatcgtt
     1741 tttggaaaaa gctgtttgat ttcttctgga aatgcgcttg ttgcgatatc cacatcatgg
     1801 attggctggt tcagcaagat atcacgaaca cttccaccta caaaataagc ttcatatcct
     1861 gcattctcta attttttcag aactggaatt gccttttggt attccagagg taattgtttt
     1921 aatttcattt ttccaacctt tcctgcgtgt ttcttgtcct gagataaaga ctcagttgca
     1981 tggtgactgt ttgacaagtc cgtttcagag tcttcaaggc caggaccact tcatcttgtt
     2041 tactcgtcct tacgctcaaa gcgattatgg tctctttgat tgaatttttc catgtttttt
     2101 tcaaaaactt cagttaagtc gatattcaat gaattagcca tgatcatcgt aacgaataaa
     2161 acatcaccaa gttcttctgc taccgagtta actggttcag aagccttttt gcttttttct
     2221 ccataagtgt ggttgacttc tcttgccaat tcacctactt cttcagttaa tcgagccatc
     2281 tgagctaatg gagagaaata ccccaccttg aattgctgga tatattcatc cacttcctgc
     2341 tgcatactgt ataaagaacg ctttttatcc atctttttcc ctccaaaact atcttaccaa
     2401 aactttaacg caaatcctag ataagagcaa ttgagaaaat cgaatttcca tgctacattt
     2461 aggaaggaat ttctaaatgg aaattctgtg aaaatacata attgaaagct ggaattcata
     2521 tggtcaaacg aaaaacagta aaagaagttc tttttatcat tataggaaca agtatttatg
     2581 catttggtct tgtttacttg aacatcgcta atcatttagc agaaggggga gtctcaggga
     2641 tcaccttgat cttaagagct ttatttggta ttgatccagc ttatacgact ttattaatca
     2701 acatcccatt gattttacta ggaggaaaga tcctcggtaa acgttccttg gcctatactg
     2761 ttctaggtac actttctttg tcattctttt tatggatctg gcaaagggtt ccactagaaa
     2821 tcaatttaca gcatgatcta ctgatcgttt ctctacttgc gggtttagtt gcaggtgtag
     2881 gaagcgggat cgtctaccgc atgggtggaa caacaggcgg cagtgatatt atcgctcgga
     2941 ttttagaaca aaattacggc atcactatgg ggcgttccct attggcattt gatatcatcg
     3001 tcttacttgc ttcattaacc tatatcgact tgaaacgtat gatgtatacg ttgattgttt
     3061 cgtatgtttt cagtcgtgtg attgattcga tcttagatgg cggatattct gcaaaaggtc
     3121 tacttgtcgt ctccaacaaa agcgaagaga ttgcaccact actgatgacc ggtctgcaaa
     3181 gaggtgtgac tttcctttct ggagaaggcg cattctctgg taattcaaaa aaaatcatct
     3241 atatggtcgt tagctctagt gaattaactg aagctaagag gatcgtccac gagattgacg
     3301 aaaaagcttt cctttcaatc atcaatgtgc atgaagtgga agggcaaggc tttacttatt
     3361 tgaagccaca gacaagatta ctgaaaaaac tataagaaaa aagacaaaga taggctatga
     3421 ggaaagattc cacatccagc tgacctttgt cttttttagt actatactat ttttaactaa
     3481 ttgttatgtt cacttacccg gttacttttc ttttgtgtct gcaaaaggat ctttcggtta
     3541 acttcatcag ataatttcaa aaaagcatcc ctatctccat cttctagtgc ttgatcgatt
     3601 tgagcataaa gcatttttag ttttgcctct tcttcttttt cttgaaaata tcgttcaatt
     3661 aattcagtat cttcttcact gactgaagaa tcccaagaag aaaatggatt gtcttctaag
     3721 atagatagat attctcttgt ctgccaagca ttttcgaata tacattccac atataaaggc
     3781 tttttccaat tcaaacggat ttcatgaaaa atctggtctg tgtccgtaaa ttcttttccc
     3841 tgcaaaaaca atttcatggg ttccccttca acagatagat cactgatctg cagtcctcga
     3901 tccgcaaaat tcgccccttc aacaaagtgg acattgctta aaatcgcttc atgattggca
     3961 agataattca agatccaaaa aacttctctt cggctaaaag atacatgatt gaccatccac
     4021 gttaaaaaat tcttcttttc ccgtacatca attagcattt ttctcacctc tctgttaggt
     4081 catctaataa tgacagtgcc tccatatctc ctggttcaag tgttaaataa taagtcaata
     4141 attcttgcgc tcttttcagc tgcccttctt ctcttaagaa aaaggcatac tctttcaaga
     4201 aatctggttc atgtgatagt tcatgataag cctgttcata gtgtacagct gcaacggtaa
     4261 agtcttctaa ttcattataa gcatgggcta ggttccattc tgcataagga ttagcagttt
     4321 cttccatctg attgatcact tgtatcacat cttcatatct ttcatcatcc aagtaaagat
     4381 tacttaaagt aagtaatgtt tcatcttgtt tgtctcctac ttctaaagct ttcagtaaaa
     4441 gttcttctgc tcgctgtcta tcatgcagac ggaaagcatt ttccgaggcc aggtgataaa
     4501 gttcgacttg gtaaggattt tctttgattc cttcttctaa cactgtttgt gcttcttcaa
     4561 tcatttcttc ttcttgaaga gcttgtccta gatatagata taacgattga taatgcggat
     4621 taacttctcg aagttcttcc agatagttga ttgcctgctg attttctttc aattgaagat
     4681 tcgtaaaagc tagctggaac agtcgatcat ctgtcctttc ttctttcagt gcttcttcta
     4741 aaggtacgat agctgcttcg aattctcctt gcatacttaa ggattgtcct aaacgttcct
     4801 gcatcgagat tcctgaaatt tcatttatcc cgcttgccaa caatgtttca taaaccactg
     4861 ctgcctcttt gaaacgatca actgaaaaat acaattcagc tagcgcaaac tggatcaatg
     4921 gttcatcagg aagcaaccca gctgcttctt tcaattttgc ttcactcact tcagggattc
     4981 cgataacttg gtacaaatca gctatcgcca ataagctttg cggatagtaa tcactttgct
     5041 tatccacttt ttccaggtat tcaaaagcta agtcgatctc attatcttcg attgcaattt
     5101 ctgctaacgg gatgttcagt ccgtcatttt ctgggtattg gttgatcagt tgttcaaaaa
     5161 tctgttttgc ttcgatcaaa aagccaatag ccagcaattc ttcgcctagt tcagctaata
     5221 tatctgctgg gtcttctttt aatgcttttt ctaaaaagat ttgtgcttgt gacaagtctt
     5281 catctgataa agcttgcaac attttttcgc tatttgtatt catttcaatt ccctcttatc
     5341 tcactgacga tttgttgaaa taccgtcatt gttctcaata attctttttc gtttggatgt
     5401 tggtatccgt ctattatacc tattttttct aaaacaagta tttttttgct tttttctgcc
     5461 aaaaaaagaa ctttttccaa atactccatc tttgaaatct gttccagcca gtggattgga
     5521 tagccaagca agtccagcca cacaaaaaag ttttttagtt ggaaatcaaa aggatatgct
     5581 tggagattcc acacgaaatg aaacatcatt cctaaaaatt ttttcatact agcagaaagt
     5641 agatggccat tttcggtcag ataaaaagct tgttcaaaca gtttaccata atttgacagg
     5701 ttttctgcct ctaattgata aaaatagatc atttcttcga tcatcccggc aaacggccga
     5761 gtcgttaatt gctttttatt tggatagttt ttatacagat tttgcaaaaa acgatgatca
     5821 caaatcattc cgcaacagat aaaagtcaaa aagtcaatct gctttccgtc cacctgccgc
     5881 tcgctgattg tttgatcata aacgatgcgt tctggcagat tgatggtttg cagcactaca
     5941 cgatttggta gctgcaaaat cgtccgctgc ggtgttaatg ctcggcttag agaacggatc
     6001 gatacaggga tcatccatag tgatgaatca agggttgtat gtttttgaaa aaaacctgcc
     6061 agatccatga tcccttcatt tccaaatccg atgataaaca tggattgatt ttcgggatag
     6121 cgtttgacga aatcaagcag gttctttaat tctgttaaat gattgcattg agtgttggca
     6181 caaatatacc agtcattgtt tgcttgattt gcaaataacg ggttgatctt atctgcaaac
     6241 aaatcataat agcgctgatt cgttaaaaat aagattggct gatcaatgga aagttctctt
     6301 agctgactga ctagcgtttc tccgtaaaac actttcgtca acgcatcttc tacttgataa
     6361 tcaagttcca tacgcatccc tcctcttctt atttttattt taccattagt aagcaaattc
     6421 tagaaaagat aagagcaaag ctcacctcat aacttggaaa taaataaaaa ttaaataaaa
     6481 tacatgtgtt ttctatgtat gacatacgga ttcggaatct ctgtctctta tttattcctt
     6541 ttctgctagg aaataaaaag aaaagcactt gtagccctag agccacaagc gctttttcaa
     6601 ctgtcttatt taacagcgtc ttttaatgct ttacctggtt tgaatgcagg tactttgcta
     6661 gctggaattt cgatttcttc accagtttgt gggttacgac ctttgcgagc tgcgcgttcg
     6721 cgaacttcaa agttaccaaa accgattaat tgaacttttt cgccgttagc taaagcatct
     6781 tgaatagttg aaaatacagc atctactgct gcagttgcat cttttttagt taaatcagta
     6841 gctgaagcaa ctttttcgat taattctgct ttgtttgcca tggatagatt cacctcctcc
     6901 acaagagata tgatagaaat atcatttagt taaaactttc tgagtggtcc agaagtttaa
     6961 aaagaaatat tgattaactt caatattctg ttatcacgat atcatatgaa ccgcttaata
     7021 gcaaggtttt taccaatttt cacatgcttt ttttagtcat agaaacgtaa caaacgaaac
     7081 tgtctttttc ttttgaaaaa aaaacgtcga tatggtaaaa tgcctatttt ctcctacgag
     7141 ggataattcg gatggacgtc ccttcaaaag taaatgcttt tctgatttga ttttctaaga
     7201 aacgttcata agaaaaatgc atcaattcct cttcattgac aaaaacgaca aatgtaggtg
     7261 gtttgacagc gacttgtgta gcataaaaaa tcttcaggcg ttttccttta tctgttggtg
     7321 ttggattgat tgctacagca tccataatga tatcattcaa cacagccgaa gagatacgaa
     7381 gattctggtt catacttact tgttcgatca tttcaggcag acgttccaac cgttgtttag
     7441 tcactgcaga aacaaaaacg attggagcgt aatcaagata ccggaattca tcacgaatct
     7501 cttgttcgaa atcacgcatt gtattggttt ctttctttac aagatcccat ttattcacga
     7561 cgataataat tccacgacca gcttcatggg cgtaacccgc tactcgcttg tcttgttccc
     7621 gaatcccttc ttcagcattc agtaccatca aaacgatatc agaacggtca atggcacgca
     7681 ttgcacgcat cacgctgtat ttttcagtat tttcgtatac ttttccgcgt ttacgcatac
     7741 cagctgtatc tatcattaag aatttctgac cactttcaga aacgaagtgg gtgtcgattg
     7801 cgtctcgggt cgttccttca atatccgaaa caattacacg ttcttctcct aaaatcgcat
     7861 tgatcaaaga ggatttacca acatttgggc gaccaatcaa actaaactta atcgtgtcgt
     7921 cgtcctcttc ttcagtctct tcagagaaat acttcactgc ttcatctaaa atatcaccaa
     7981 tccctagtcc atggcttcct gaaacaggat aaggatcgcc tagccctaag gaataaaatt
     8041 catagatatc gtttctcatt tctggattat ctactttatt gactgctaaa atcactggtt
     8101 tattacttct gtataaaatt ttcgcaacca tctcatctgc gtctgtcacg ccttcgcgtc
     8161 cgctgactac acaaatgatt acatctgctt cttcaatcgc gatttctgct tgatgcttga
     8221 tctgatccat aaaaggttca tcgccaagat caattccgcc tgtgtcgatc acgctgaatt
     8281 ctcgacctaa ccattcacct tttgcataaa tacgatcacg agttactcca ggagtatctt
     8341 cgacaatcga gatacgctca ccagcgatgc ggttaaaaat cgtagactta ccgacgttcg
     8401 ggcggccaac gattgcaatt gttggatttg ccatattatt tcctcctcac aagtttccaa
     8461 tctcacttag tttaacgaat gcattgccaa ctttcaagca tagattgtca attaaacgaa
     8521 gtatttttat tccttattga aatactaatc gcttttcaaa agaaaaactg agacaaaagc
     8581 agctgctttc atctcagtca cagatacaaa atcaattatt tttcagaatc atcggttgct
     8641 ttttcttcta cttcttcttt caacgcatca cctaaaatgt ctcccatagt aaatccagta
     8701 ttttcttctg gtaattcata agactcaaca ttttttggtt cttcttctga agctggtttt
     8761 tcttctaaag ctttgatact caatgcaata cgatgttctt ctggatggat ctctaatact
     8821 ttcactttca cttcgtcacc ttcatgaagc acttcatgtg gtgtcgcaat atgtttatgt
     8881 gaaatttgag agatatggac tagcccttct actccaggga acacttcaac aaaggcaccg
     8941 aagcttgtca aacgtttaac tgttccgtct aatactgtgc caactggagc ttttgtatcg
     9001 atttctgacc aaggtccagg ctgtgtgtct ttgattgaca aggaaacacg ttcttgttca
     9061 ggattgatag acaacacttt gacatttaca tcatcattga cagccaatac atcacttggt
     9121 tttgctacat gactatgtga aatctctgag acatggacta gaccgtcaat tccgcctagg
     9181 tcgacaaatg cgccaaaatc agtaagacgt gcaacacgtc cactgagaac atctccctca
     9241 tggatagttg aaagaatttc ttttttcttt tcttctcttt cgttttccac taccgcttta
     9301 tgcgataaga tcaaacgatt ttctgatggt tcgatttcga tgattttaaa gctcaatgtt
     9361 ttgcctttat agtctgaaaa gtctgcaaca aaatgatctt ccaccattga tgcagggaca
     9421 aaaccacgta caccgacgtc aacgactaaa ccgcctttga caacatttgt aacaggtgct
     9481 tcgatgacct tgccctcttg gaaatctctt tcgatatctt cccaaacttt tttagcatct
     9541 aaacggcgtt ttgaaagtag gtagctgccg ttttctttat ctttgccgat cgtactgata
     9601 acgactaaat ctaatacatc accgactttt actacttcgc tgatatcttc taccggcaaa
     9661 gttgataatt cttttgccgg aacgactcct tcaactccag ccccttcgat tccgacaatc
     9721 acttgtttgt cttcaatgac taaaacttcg cccttaacaa catcgccaac ttttacttct
     9781 tgtacgcttt tcattgcgtc ttccattgac tggttgttct cttgattttg atcaaattct
     9841 gtcataacac ttgtcctcct aaccaacatt ctgccgtaag tacggtactg tttttagttt
     9901 aattgattat tcaaaaaaaa taaagcgatt tcgttcgttt tccttgaaaa aacaattgtt
     9961 tttcattttt tttcttaatt taaaaaagaa gataaccttt tttcaaaaca acagctttta
    10021 ttgctgcaac gacttctgga atcgatttgc cagtcgtatc gatacgaaca gcatcctctg
    10081 cttgcttcag cggagagacg tcccttgttg aatcataatg gtctctttcc tgtatctctt
    10141 tcgtcagtgt ttcaagatca gtcggaattc ctttttcttg gttctctttg aaacgtcttt
    10201 ctgcacgctc acttacgctt gctactaaaa agattttcac ttctgcattc ggtaatacag
    10261 ctgtcccgat gtctcttccg tccatgacaa cgccaccgcg ttcccctatt tttcgttgga
    10321 cagcaactaa ttcttctctg acttttccat gtgcagagac ttcagaaaca gcttttgtga
    10381 catctggttg acggatttct aaagtcacat cttttgatgc tatgaataca tgctgtccat
    10441 cttcttgttg cgaaaaagag ataggatact ttttgatcag cgaaactagc ccttcttcat
    10501 ctgaaaaaga tacattgttc gtaatagcta aataagttac tgcgcgatac atggcaccgg
    10561 tatctgtata aatataattc aattcttttg ctaatatctt ggcaacggta cttttcccag
    10621 atgaagcagg cccgtcgatt gcaatactga tttgtgtcat gatataactc ctttttctca
    10681 gaaaaaaagc cgcaaagcgc ggctattatt atttaattct taattcttga ccagggtaaa
    10741 gcataaagtt gtttggatct aagccattca attcaaacaa ttgatcagtg gtgatccctg
    10801 cacgctcagc cacttgtttt ggaccttcgc ctgcttgaac agtcgttgtt gaaccggctg
    10861 cagctgatga agaaggtgtt gtttctgctg cattgctgct tgaatcccct gcttgataag
    10921 ttgcctcact tgaggaagtc ggtgttgtct ctgctgcaat actgctttct gacgttacac
    10981 ctgtatcttg agacacagta gattcttccg cgattgtact agattctact gttgaagtct
    11041 cagttgactc ctcttttgtt gtacttgtag aactttctac tactgatgtc gaagtttcag
    11101 aagaagttgc tgctgttgca ttgttgcttg atccatttct gatccagaaa taagcacctg
    11161 ctggaatagc gatacataag accagtaaaa tcaaaaatag cgaaagaaat aatgtatttc
    11221 ctttcttttg ttgtctctga gaacttcttg atgaaacttc atcatctgta tcgtagattg
    11281 gttgttccca tggttcttgt gtttcgttat tttctgtaga ttgatgtcta tcttttctgc
    11341 tcactgtttt acctcctaaa ctctatcgtg tgtattatac cataggaaaa agtgtcttgt
    11401 cctaaaaaat caatgttcca tgtttttttt aaataaattt agtaaaatcg tttcccaatg
    11461 aagttgttca gtcttagaaa aaagcttcct ctttggcaga agttctgctt ttgctccatc
    11521 aatatcacaa caattctgcg gcttttcgga gagtttttcg ccaaaataag ctaagatgaa
    11581 ttttctgcgg cagttttctt cattaatata gcgaagcatc tgctgcaggc ggaattcttt
    11641 ttctttttcc tgacgtttga gccgttccac ccaattttct ggtttttcag actgctggat
    11701 cagctccatc catttttttt gcagctcatc aaagggagct tgctttgtgg catattccaa
    11761 ataaagctca aaactttgtc gctgttctct agaaagttga ttaaagaaat aatggatacg
    11821 ttcatcccca ctttgataaa aaagaattgc tgcactttct tcctgatctc tgcctgctcg
    11881 accgatttct tgtacatagt tttctagact gtctggcaaa tcataatgga ccacataacg
    11941 gatatcagat ttatcgatcc ccataccaaa tgcattcgta gcaatgagaa actgcagctg
    12001 attttttaca aactgctgtt gaagttgtct tctttgagct gcatcaagac caccgtgata
    12061 gtatccaact gtaaaacgtt ccctgaataa atgatacaat cgttcgactt ctttttttgt
    12121 tgcacaataa atgattgccg ctccttgtgc tctttccatg aagtgttcaa gttcttgttc
    12181 tttttgctgc gttttccgaa caaaaagcga gatattttgc cgattgactg attgtctgat
    12241 ctcttttggt ggtcgatcaa atagtacatg acgtatatct cttgctacta aatctgttgc
    12301 tgtcgccgtt agtgccagtg tgaccgggtt tcctaaaagc tttcggattt ttcctaactg
    12361 ttgatattct ggacggaaat caactcccca ttgagacacg caatgagcct catctactac
    12421 atataacgca atattttgtt ttttcagatg ctctaaaaga gaaggattag tcaacatttc
    12481 gggactcaaa aacaaaaatt tatattgcga tagatgacgg aggacgtatc ttctttcaga
    12541 ttgcgaaagt aaactattga aagcagctac tcgtttctcc cctcgttttt gtaattgcgt
    12601 cacttgatct tccattagag aaagcagcgg tgacacgatg atgaccagac cttccattaa
    12661 ataccccgtc aattgatagc aaaggctttt tcccgttcct gtaggcaaaa tagcgagagt
    12721 atcttctcca tcaagcaacg cctgaataat ttcttcttgg ccgggacgaa atgaatcaaa
    12781 tccaaaatat ttttttaatt catttattaa cattctgtat cgcttccttt ctcaatagat
    12841 agatttgata caaacgaaat tctccataat ctaattgtcc atctacattc cattcgctaa
    12901 agcgccactc tcgcacggaa tccttatttg gttccaaacg ttcgattgtt tctgcagaga
    12961 gaatccactc gtatggaaaa tcatcaaaaa acaaggccca ctcaataaga tgatctgtta
    13021 ctgttcctct ttttaaatga cggattgcca tgatctggtc aattgtctga ccattcataa
    13081 acatctgctt ggtgatcatc atgctttgat tgaaattttg ctgtaataat gaatcgatca
    13141 aagcgtgcca aagcgaatct ggccgttttt cgatttgtgc taacaaaagg tggatacatc
    13201 tgctttgttg cagataaacc tctgactcat cattgttttt tgccagctga taaggaagca
    13261 gccctgttcg ctcatttcca gataattggt tagctaaaaa atctgctgct tcttctggta
    13321 tctccgaaaa cagctgcacc aaactctcat acgcagtatc aatcaaaatt tcacgtggca
    13381 attggctttc actaagccat ttttttagtt ggaaagtata aaaaggactt gtctctgctg
    13441 gaagatagtc ttgaataccc gttgccagat tagaaattac ttgaacagca aatttaatga
    13501 gccgccaact agtttcccct gttctgccat agcggtcaaa acgcaaacca gtatgttcta
    13561 tttgatgttc ggacaacata tcagcccctg caggcgttag ctttgcttca ttttcattta
    13621 tttcgatcca accttgctgc gcaatttttt gcaaaatttg ataaaactta tcctgcttca
    13681 attctggaaa actgttatgg ataaacaaaa gttcgtgggt aaatccataa ataagtactg
    13741 aagtcgttcg ttttcccaca aggagttggt aaagagaact aatccgcagc ttgtgtcctg
    13801 atgaaaatag cgataaaata aaaacaatca tattattctc cttaagttag gatggttaga
    13861 aatgtcacta ctatgtaaac ttgtacctga acgctgtatt gcatgcggtc tttgtcaaat
    13921 cgaagcgcca gatattttcg attatgatga agaaggaatc gttttatttg cccaagaacc
    13981 agaggctcat caacagttcg tgatgccgga agaagaaaca tatgtaaaaa aagcgtatca
    14041 aaaatgtcca gttcgagcga ttttattaga aaataaaata taaatataaa cgaaacttta
    14101 aaaacgtaca ctaagttctc taaacttgaa aataagccgg gatctgtttc tcttgttccg
    14161 tgaaatcccg acttattttg aaagattcat ttgctatcta tagttaattg actagttttt
    14221 tcacggaccc tataggaccc atttatatta tttcaacttt ttcagccagt tttccaatgt
    14281 attgacgatc acagtttgct ggtctgcgtt agaaatttct gcctctccat ctcctttttg
    14341 ctcaccatag ctgccaaaac ctccatgatt tccgccattg attgatacat actctgttct
    14401 ttccggtaaa tactgcttag cttcttgata agcttcctga ttcaatactt tatcttttgt
    14461 cgccgtgatc gataaaacag gaacagctga ttctgtcaat gttccttttt tgtcgggata
    14521 acttgccatg aagaataccc cttttaaatg tttgtctgaa tgattcttat tgacataacg
    14581 acttgccatt acgccaccta atgaatggcc accgatcaca taatcggaat ttggaaaatc
    14641 ttctttgacc ttatccgctt tgtttccgcc catcactgct agatcaagtg gcattttgac
    14701 aatagcgact gaaaagccgt tttttgctaa atcttgcgcc caaatactat aactttctgg
    14761 ctctacgaga gcaccaggat aaaaaataag taatggagac tttccatgat tctttttctc
    14821 tccttcaaaa aataagtaat cttctttgtt ttttccttct tttaccaaca cttgcgcttc
    14881 tgacgatggt tcgtacgtat tttgctgtat atagtaataa ccggctccac cgagtaatcc
    14941 taaaatgatg actaagccaa ttagtattcg tctaaatatt ttcactttct cccacctttt
    15001 ctatctgagt gacccctttt tcttaatcaa gtatgattat accttaaaaa aataagagag
    15061 aaatgcaaac tttccagcat caaataaaaa aagacggaat atccatttga atatccgccc
    15121 tttttgtatg tttattttta tttagcacat aaacgataca cagcttctgc ataaatttcc
    15181 attgctttat acatactact taattcccat cgttcattcg cctggtgcat aaagtcagga
    15241 gtatcaggga acatcgcacc gaatgccaca cactggttca tcgtacgagc aaaagtagcg
    15301 ccgcctgata caaacggttc agtcatgtct ccagtaatat cgcgatatgt ccccaataga
    15361 tttttcacta actcactgtc tagtggcacg tataatgaat caaggaagtc aaattcttca
    15421 taagtcaacc catatttttc tgctgtttta gttaatttag ctactaaatc atctttcttg
    15481 aatgtaacag ggattctcat atctacaccg atttttgttt gttctggtgt gatttccaaa
    15541 cttgcaaagt tcatcgttag ctcgccggat tgttcatctt ctgttttgcc cacgacagct
    15601 tcaccagttg cattttcttg aactaatttt cctaagaaat tgattgggcc aaaatcaaat
    15661 acttctgaaa gagcaattgc cagacgagaa atagcattca ctccttgtgc tgcatctttc
    15721 gcgtggatac ttttaccaag aacgacgatg ccttcacctt gttcttcgaa atcgaattcg
    15781 tgttttttca atgcttcttt tacttcagaa agcttttctc cagagtaagg agctgcatca
    15841 ggaacaacat tcaacgctcc tccagctttt acagaaaact cgttcgttcc tggtcctgtc
    15901 aagtaagctt gcaataagcc tttttctgca taaattaaag gaaattcagc atctggcgca
    15961 aatccttgcg taattccttc ttctttttcg ttatattttt ctaaacaacg ccataaattt
    16021 tcttcatctg taccaaagat aaaacgaata cgtgtcttga attccacgcc agcatcaatc
    16081 aaagctttga cggcatacat tgccgcaata gaaggacctt tgtcatcttg ggatccgcga
    16141 ccaactaacc agccgtcttt tatagttgga tcgaatggtt ttgtttccca attattttcg
    16201 tcccctgctg gaacaacgtc catatgacac aagataccga ataattcatc tccagagcca
    16261 atctctgaat aaccgtaata gccttctgga tctttaaatg ttctaaatcc tagcttttca
    16321 ctgatttcca atactttatc caaagcttca atcacttttt taccgaaggg atgtccttgt
    16381 cctgaatctg cttcatctag tacggaagga attcggatca attcgctcaa tgattctaat
    16441 gcttgttcat gatgttgttc tgtcacaaat tttttcatga tattatcctc ctaatatgtt
    16501 aaaaagggtc atgtcaaatt cctccgacac aacctcttct tctcaaataa tgacgtttta
    16561 agcctgctcc atcagcaagc agaatgttct taacaaatga tgaaagaaaa aacactcact
    16621 gaatggagct attgacttgt ccacgtgctt atcgatagcc gtttatcggt ttcttcattc
    16681 ttatgataga ccttgtacct gtatttgtct aggtttagaa cattgctgct acacctagga
    16741 tcacacagat ggctacaaat aatatcgcaa ttaatttacc ggtaaatttc caccacactg
    16801 aaacatcgaa acgagcaatt gctacagcac ccattacgat agcagatgtt ggtgtaatta
    16861 agtttactaa accagatgca gattgataag cagtgattac taaatcctta ccgacgccgg
    16921 caaattcacc cataggtccc atgattccca ttgttgcagc agctaagccg gaagttgaag
    16981 gaatcaagaa tgacataggg atatagaaaa taaatgtaat aatgatgaat acacttgaag
    17041 ataatgaact taatccttgt tcgccccaat gtaagatggt gtctgtgatt aatccattgt
    17101 tcataaccac ttggatacca cgagcaacag ctactacgat tgctacaccg atcaaatcag
    17161 aagcaccagc taaaaattca gagacaaatt cagactcttt catgccataa gtgattgcta
    17221 ctacgatgga cattactaag aataacatag taatttctgg gaagtaccag tcacctaatg
    17281 gaagcatgct tttacctaga accgtaccta gtaccggaag gtttgttaac cagtttgtca
    17341 atgattcaaa aatcgtgaaa ttcgcattca aggttgtcca aggaatcaaa ctgatgatca
    17401 tgataccaaa agtaataaag aacaatacat taacatgttt ttgttttttc gtgattactt
    17461 cttgacgacc gattgcttca atatcgaaat gcttcatatc ttctttacgt tggttgtata
    17521 ctaatgattt tgttggatct ttttcgatct ttgatgcata acgatacaca tatacaatag
    17581 aaatagctaa caaaataacg aacatcaaga atcgaagacc aataccttca cccatcgaaa
    17641 tcccaacagc ttgtgaagct acgcctgttg caaaaggatt taccgttgaa gctagacagc
    17701 ctacttgaga ccctactaat acgattgcta cagctactac tgtatcaaag cctacagcca
    17761 tcatgactgg gatcagtaaa gcatagaatg gaatcgtttc ttctgccatt ccgtaagtag
    17821 agccgcctaa tgcaaataac agcatcaata ctggaataag cattttttct tttcctttga
    17881 atttcttcac aactgaagca atcccggcat ctagtgcccc tgttttgttg ataataccta
    17941 aaaatccacc aacaaccaaa atgaagaaag agattgggat tgcagcaggt gtttctttag
    18001 ttagctcccc cgctggtaca cctagcatcc cattgattgg cgccatgaaa atatcccaca
    18061 gcccttgagg attttgttgt actcgttcat atgatccagc gacgatgttg cctgcatcgt
    18121 ctacactata gtgcccagct ggtataaacc aagtaagtac agcgattact gcaataataa
    18181 taaataagac cgtatacgct gaaggcattt gaaatttttt cttttttgtt tgttccatct
    18241 tcttcttcct ttcttttccg ttagagacaa caattttggc tctaatgtta ttttatcaaa
    18301 aagagtatca gaacaaaaat aataacacgc attttgtaaa acttttgttc aaaaagttcg
    18361 tttttcccta ttccctcctc ttttttcagt tttttaaagc tttcttcttt ttcattgtag
    18421 cgtttccttt tttttataaa caaacatttt cgtcttttaa aaggagaaga atttcacatt
    18481 tgcaaagaat tgattgattt ttatgcataa atcaaaaaaa tactcaaaac aaacaaataa
    18541 aacgtttaca ttattgcatg tctt
//
LOCUS       DACDEG010000046        17932 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.048, whole genome shotgun sequence.
ACCESSION   DACDEG010000046 DACDEG010000000
VERSION     DACDEG010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.048"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(51..1517)
                     /locus_tag="IV142_001636"
     CDS             complement(51..1517)
                     /locus_tag="IV142_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP8485300.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1904..2500
                     /locus_tag="IV142_001637"
     CDS             1904..2500
                     /locus_tag="IV142_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8485301.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2558..3040)
                     /locus_tag="IV142_001638"
     CDS             complement(2558..3040)
                     /locus_tag="IV142_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485302.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3146..3343)
                     /locus_tag="IV142_001639"
     CDS             complement(3146..3343)
                     /locus_tag="IV142_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485303.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3638..4396
                     /locus_tag="IV142_001640"
     CDS             3638..4396
                     /locus_tag="IV142_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP8485304.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4456..4884
                     /locus_tag="IV142_001641"
     CDS             4456..4884
                     /locus_tag="IV142_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485305.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4930..5002
                     /locus_tag="IV142_001642"
     tRNA            4930..5002
                     /locus_tag="IV142_001642"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4963..4965,aa:Gln,seq:ctg)
     gene            complement(5152..5523)
                     /locus_tag="IV142_001643"
     CDS             complement(5152..5523)
                     /locus_tag="IV142_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8485306.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5738..5923
                     /locus_tag="IV142_001644"
     CDS             5738..5923
                     /locus_tag="IV142_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485307.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6006..6194)
                     /locus_tag="IV142_001645"
     CDS             complement(6006..6194)
                     /locus_tag="IV142_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485308.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6218..6481)
                     /locus_tag="IV142_001646"
     CDS             complement(6218..6481)
                     /locus_tag="IV142_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485309.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6639..6896
                     /locus_tag="IV142_001647"
     CDS             6639..6896
                     /locus_tag="IV142_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP8485310.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6967..7158)
                     /locus_tag="IV142_001648"
     CDS             complement(6967..7158)
                     /locus_tag="IV142_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485311.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7299..7619)
                     /locus_tag="IV142_001649"
     CDS             complement(7299..7619)
                     /locus_tag="IV142_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485312.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7642..8076)
                     /locus_tag="IV142_001650"
     CDS             complement(7642..8076)
                     /locus_tag="IV142_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8485313.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8411..9049
                     /locus_tag="IV142_001651"
     CDS             8411..9049
                     /locus_tag="IV142_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8485314.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(9373..9456)
                     /locus_tag="IV142_001652"
     tRNA            complement(9373..9456)
                     /locus_tag="IV142_001652"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9420..9422),aa:Leu,seq:cag)
     gene            9984..10484
                     /locus_tag="IV142_001653"
     CDS             9984..10484
                     /locus_tag="IV142_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8485315.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(10674..11018)
                     /locus_tag="IV142_001654"
     CDS             complement(10674..11018)
                     /locus_tag="IV142_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP8485316.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYVRIALGIR"
     gene            complement(11172..12092)
                     /locus_tag="IV142_001655"
     CDS             complement(11172..12092)
                     /locus_tag="IV142_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8485317.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            12279..12770
                     /locus_tag="IV142_001656"
     CDS             12279..12770
                     /locus_tag="IV142_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP8485318.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(12794..15169)
                     /locus_tag="IV142_001657"
     CDS             complement(12794..15169)
                     /locus_tag="IV142_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP8485319.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            15495..16121
                     /locus_tag="IV142_001658"
     CDS             15495..16121
                     /locus_tag="IV142_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8485320.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            16144..16848
                     /locus_tag="IV142_001659"
     CDS             16144..16848
                     /locus_tag="IV142_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8485321.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            17040..17873
                     /locus_tag="IV142_001660"
     CDS             17040..17873
                     /locus_tag="IV142_001660"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP8485322.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         5568 a         3492 c         3019 g         5853 t
ORIGIN      
        1 aaaaacagga actgctgcaa ataagcaatt cctgttttta tgttcattga ttaatgaata
       61 gagcccatta gttttttgat tggttctttc acagtaaata gcaagatacc accgacgatt
      121 gctactgcac cgataatacc gaagtaagca gattctgttc ccggtgtgta gaagcgtgca
      181 atttgtgcat tgattgcttg tgaagaagca tcagctaaga accaaatagc gattgtttgt
      241 gattcaaatg ctcgtggtgc taattttgtt gtcacagaca aaccaactgg tgaaagacac
      301 aattcagcaa taaccacgat gaagaaactt cccaacaacc acaatggact tacttttcca
      361 cttgtaccat aaagcatacc cggcaacatc aatagcaagt acgataatcc tgcaaataaa
      421 agtccaagag agaatttcac tactgtagat ggttgacgtt caccaagttt cgtccacatc
      481 gttacgaata ctggtgtcag aataaagatg aaaattggat tcaatgattg ataccagctt
      541 gcttggatcg ttacaccaaa tagcgtagat tgtgtacgtt cactagcaaa taaagctaag
      601 atagatgaac cttgttcttg taatgaccag aaaatgattg ctgcaaggaa taatgggata
      661 taagcccata ctcttggttt ttcttcagca gttacatctt ttgaagtcag cattttgata
      721 aagtaataca acggtaagat aatccccaat acactgattg tattgacaaa aaagtcaatt
      781 gtcagatgac cagttagttg tgccccgccg aatactgcag cgataacaac aagtgcgatg
      841 atcaagttac ggatgaatcc tttacgttct tcagttgtca aaggattcgg cggtgttgta
      901 cccactccgt ctaatgtctt gcgtccttgg atgatgtatt gtaataatcc aaagaacatc
      961 ccaatagcag ctaaagagaa ccctaaatga tagttatatt cttgaccgat tgtaccaacg
     1021 attagtggtg ctaataatgc ccctaagttg acacccatgt aaaaaatcga aaatcctgca
     1081 tctcttcgca agtcagtctt tgaataaaga tgtccgacca tgccagaaac atttggtttc
     1141 aaaaaggcgg ttccgataat aatcaatgcc atcgaaacaa acaatgtact tacaccaaat
     1201 ggtaaagcca agacgatgtg gccaagcata atgaaaactc caccgaagaa tacagtgcgt
     1261 tttgctccta acactcggtc agatacccat ccgccgacga tactagacat gtagatcaaa
     1321 gatccgtaga tcgacatgat agctaatgct gttgctcgtg gtaatccaag tcccccgttg
     1381 gcaactgtat cgtacatata atagataagt aatgcacgca taccgtaata actaaatcgt
     1441 tcccacatct cagtgaagaa caaagttgac aatcccttcg gctgcccgaa gaagcccttg
     1501 tcttgctgtt tttccataat ctatacccca attctttaaa ttttcagaaa atattataca
     1561 tctttaccga aaaatttaac taaaaaactt atttaagaaa tataaactaa ttttctataa
     1621 aagcaagtaa tttttaatgg caatatcata aaaaaaagaa gatagagatt taataattat
     1681 atatcagctg tttttttcaa ataagcaaaa attcctaaaa cacaacaatg aaagcgataa
     1741 ctaagaaaat ccttacataa cgatttttct tattaaaatt gttttttgaa cttcgcttat
     1801 tttttgtgag catatctgtt tacttacttt ttgtaaggtg ctatacttca tttcgtaatc
     1861 aattaaaaaa atgcaaaacg caaatcaaag gagaaatttt attatgacaa cttttactga
     1921 cactttgaaa aaccgccgtt caatctatca tctaggacgt aatgtttcac tttctaatga
     1981 agaattaaca actttgatca aagaagcaat taaagaaagc ccaactgctt tcaatgctca
     2041 atcaacacgc gcagttatcc ttttcggaga tgcacatgaa aaactttggg aaatcactga
     2101 agaagcactt cgcccattga ctccagcaga agctttccca aatacacaaa acaaattagc
     2161 tggtttcaaa aatggttatg gtacagtatt attcttcaaa gatactgacg tggtaaaagg
     2221 tctacaagaa caatttgaat tatatgcaga caacttccca gattggtcag aacaatcaaa
     2281 cggaatcgca acagctaata cttgggtagc attagttgat aaaggattgg gcgcaaactt
     2341 gcaacattac aacccagtta ttgacgaagc agttgcaaaa gaatggaata tcccttcaaa
     2401 ctggaaacta cgttcgcaat tagtattcgg ttcaccagaa acaccggctg gggaaaaaga
     2461 atacatgaac gatgccgatc gcttccgcgt atttggataa ttttatttga aataaaaaac
     2521 agaggttgtg acaaacatct tgtcgcaacc tctgttttta ttctggatca atcataatat
     2581 caatctctgc ctcatcgttt aagcgttggg acaaaaatgg aactaattct tctaatgatg
     2641 cagaaaattt cagcatcggt cctcgccatc cgtttcggaa aaaattcatt tttacgattt
     2701 tagaaccttt tggctgatat aatacaattt tattgatctc agaaaaagga acaccttttt
     2761 tgtcaaagct attcaaaaca agtccttctt ctgtgattcc tctactgtcc aacaaaaaac
     2821 taataaaaat cattgctgac aagatccctt ttgccaattc atctgctgta ggatgactga
     2881 tgagtgtata ccaaatgact agtgacacta agaagggtat caccaagtag acccatcgat
     2941 ttcgcacagt ccgataaata atttcttttc gaaccagcca aagaaataaa aacatcacaa
     3001 caacgaataa aaacaataga accgtaaaga atgttaccat gacaactcgc ctcgcttttt
     3061 cttctccaga aaataggaaa agaatactga tttaccttct caaagaaatt ggacttgcag
     3121 cacgccgcac gtccaattgt tttctttatt caaatgtatc atattttgca acaaccatgc
     3181 ttgctttttc atctaataat tcttggtcct ctgcagcaca ttgggcaact ttgaccacgg
     3241 cgcagtttgt catttttgat acaacttcac caatgaccgt ttttgtaaaa ccaattggtt
     3301 tgcatgcata ttgttctcct aaattaatca tttcttccgt catcaaaatc ctcctaaact
     3361 gctggaactt ttctccacat taccactgct atttctttgg agatttaact cattgatttt
     3421 tatttacagt taaaaatcaa ccttacaggc attatagcgt actgttttcg ttttcacaat
     3481 taaaacagct gttgtattta gaagaatttt tttcaaaaaa ttaaaacata ttccttaaaa
     3541 actaattatt tcctacatat taaaatataa tacttccatt tccaataaaa atacatttat
     3601 aataggaaag ataaacagct gaaaagggag atttcaaatg aagaaaaaaa cgttcaaaaa
     3661 actattgttt ctagggggca cagctgctct tttattaagc ggtacaagtt attacctaca
     3721 tatccattct ccagaacata caatgaacac tgcacaaaaa agttcaacac aaaaaacgaa
     3781 aaagacaaaa gagaaagcag aagttttact agatgttcca ttagagagcc agtttgattc
     3841 tccttcttta gaaaatggct gtgaagtcac ttcgctttct atgcttttat ctttttatgg
     3901 ttatgaaaca actaaaaacc agttagctga gcaattggat tacgtccctg ttttcaatgc
     3961 agatgggact catggcgatc ctaatgaagg atttgttggt gatatcagtg gcggagactg
     4021 ggctatggtc gtctatgttc ccccagttgc ctcccttgct cagcaaatcg tccaaacgga
     4081 ttatcacact tttccaaaaa ccgatgctca actcgatgat atcaaaaaag cactatctaa
     4141 aggaaatcct gtctggactt ctgtgactat caattttgaa gtcccagatg aaactgattt
     4201 tatgacttgg accacaaata atggagaagt aagagtcact cctcttgttc atgcttgtgt
     4261 agtcactgga tatgacaaag aaaatattta tgtcaacgat ccctatggtg taaaaaatcg
     4321 agcagtaccg atcaacgatt tttcggcgat tttcactgca atgggtggac agatgctaac
     4381 tttggagaaa agctagtaat tcaaacaaaa atcccttata ctcaagatac caaacctttg
     4441 aagggagcgt agataatgga aaaattcaca cataaaaaaa tggatccaaa tgaaattccg
     4501 attatttttg tccgtgactg caagggaaat gttcaaggta aagtctctat taatgagtgg
     4561 aacgagcgtc gcagacctgc taccctcaat gaactggaaa tcaaattgta tcgtcaatct
     4621 cttgtttatt acgcagatca agagtacgag aaagccaccg atttattgaa atttctaatt
     4681 gctcgaacag agtatactcg ctttgaatat attgaacgtt tagcaaacat ctatcatatt
     4741 atgaatgaac ctgtcaaaga atatcagtta ttggacaccg tcttgtcagt agcagaactg
     4801 attgcccttc ctgctggatt agaaaagaaa ctagtacgcc gccttttacg agtaaagcaa
     4861 caactatccg atcaagaaaa ataacttgcg tttttttagg aaagtgctat gattattttg
     4921 ttgaataaat gggatgtagc caaactggta aggcacttga ctctgaatca agcaattgta
     4981 ggttcgaacc ctgccatccc aatttgatgt gcttgctgaa aaaatgaacc ccctgtaaaa
     5041 gaacaatctt tttgcagggg gattattgta ttatttttac tacttgtatt tagtttacat
     5101 aattaaatta tgaataataa aatcgggcgt attcttaata caaaaactga tttacccaat
     5161 aattgctaat gctttctccg gaactttttc tctaggaact tcctcatacg tttctatgtg
     5221 tgctccttta ttgctaattt tcaaataatg attttctttt aacacagaaa gaccattaaa
     5281 ttcaaccgtt cgttctttcc cattggcatc agcagctttc tgcacatacc ttgctgtacc
     5341 atattcattt acttcctctg gtttttgtgt ctttacatag acctctcctt tttcaactaa
     5401 tggatttagc tgatcaagta taccagggat ttctccataa cttccttcag tataatattt
     5461 cagtccaaat agcaaaatac cactaatcgc cgccagtcca atcaaatatt taatcacttt
     5521 catctttcta cctccttgtt atatattcag tatatgggtc agttaaagaa attccatccg
     5581 tcccaagttg gagatttttc tcctacttta gaagcgagaa tcgagtgttt ttttagtaaa
     5641 aatgatcgtt atttgatatt attgacgtgt ggataaatca gtttttctcg ttttgtccat
     5701 gtaaaaaatc atgttgatag caaaggatgt gttcaaaatg aatcaagaag aattatttca
     5761 aaaagtcaag gaaatgatta agaacggcaa tttcgatggc gcaaaacgat ttatcgaaga
     5821 acataaagaa caattaggac cttataaaga gaaagcccaa aatcttttaa aagatgtaaa
     5881 tatcgacagt gtcaaaaaca aatttaaaaa tctattcaaa taagtaaaac tttcactttt
     5941 gtaaaatcaa aggaggacaa tgctgctgca tcgtcctcct ttggttcttt tctgaatact
     6001 tcatactaaa ataaacgtgt cggatcgtaa tgataagtca cattgccgtt cagtgaggct
     6061 tttcccacca catatttacg attctccaac ttcagccacg cttctcgaaa agccattaat
     6121 tcttctcttt ttacatcaat cttttcgatt tttccatcga tcaaatcttg aataagttgt
     6181 tcatatggat tcattttttt cctccatctg ctgtcaatca ataagttgta aaaataggtt
     6241 catatgcttt tttggtatct agatcttctc tgactttgac tgtcgtataa ataatttttt
     6301 ctactgggat actataataa cttccttcac cattattgaa tcggtgaacg tctttctttg
     6361 ctaaaatacc atttatttta ttcgtcagta cacgttgttc gacactgtta cttacaacat
     6421 agtaaatcac ccgaatatta ttgatcaaat gcaaatcgat ttctaacact acatgatcca
     6481 tgaaaatccc actccttctt tcactttgat tatagcaagt ttctcctcta atttcaaaat
     6541 cgatacagat tttaccggat aaatccacaa aactgcttgc gccatttcgc acaaagtgtt
     6601 attttgtatc atggacttta tttatcggag gcaatagtat gatcgcgatc ggtcaatttg
     6661 ttttttatat tccttttttt attatgataa gcattctttt ctattacata aaatggacaa
     6721 aaaagaagtt ttcagtttta ctggcctctt tacctgctgt atattttact tatcaaattt
     6781 tttcttttag gcattgggaa acaacgtctg tattaataac acacatcatc gaattgacgc
     6841 tttcagtaat ttttcttatt atttggatct attttttata taagaatcag aattgaatcc
     6901 ctcgcatgtt taaaaagaaa acgattaaag atgcagcaaa tttctgccga tctttaatcg
     6961 ttttttctat tctgttatac caaaaaaatg tttgaatcgg tgaaggatcg ttccttgttt
     7021 taattctata aattcttgtg cgatcgggta cttttcttcc aaattttctt tgttcttttc
     7081 gatcaggctt ttcgcttctt catatttttt atctgcacat agtgctttta cttcctgtaa
     7141 tacttttcct gcttccattt gcctcaccct cttgccattt tattgcttta agttttattt
     7201 aagttatacg cattattcta gtttcatccc aaataacctt ttttcatttt tactcctcga
     7261 actaatcagt tttctaaagg ctgattggtt cgtctttttt atttttcctc catttcattc
     7321 tgctcttctg ttaatttatc taatgctttg attgatgctg cggctcttgc ataatcatag
     7381 ctgtataatt ccataaattt atcaggcaga acaaaccacg ctggatgata accgttagct
     7441 gtgacaggga atcctttatc ttgttggtta tctccatccc atctcagagc aacacctttt
     7501 tttcctttcc actttactaa agcgatcgag tatcccttct ttgtttgtgt gccgtcttca
     7561 ataattgcta aaatttctat tttttctttt ggcgcacgga cttcttgagg acgaaccatt
     7621 ttctattcct cccttatctc attacttcac gaccaacaca ctgcaagggg catgtgatac
     7681 gacataagaa gcggtcgatc caactaatgc ttgttgtata gcaccttttc ctgtcgctcc
     7741 cataacgatc aaatcaatat tttcttgtcc agggatgaca ttggcaatca aatttcttgg
     7801 atctccagtt tcaactattg cgtggatctc agcaatgccg tactctttgg catcattgat
     7861 ttttttcagc atggctactt ctgttctttg tttttcatct gcatagattt ttgaaaaggc
     7921 ataggcacta tgggtcagct caactttgtt gatgattgac acgataaata agtgggcttc
     7981 attgtccttt gctaagtcaa gggcttcagc gaacgctttg atcgattgtc ttgatccgtc
     8041 aactgctacc atgatgttct ggtatctttt actcattgga accgcctcct ttttctttta
     8101 ttgtaacatc tcaagcaaaa atacaaaaaa tatacgcaat atgtaaataa aatgatgaac
     8161 aaaagcaatt tctttcaagt tatttataca tttcttttct catcagaaca cctgttctaa
     8221 tcccattatt tctgcattat gtgaaattcc tcatcatttt tcacatcgag cgaacatcat
     8281 tctgttacaa aaaacgtgga tttgtcataa attgtcatgc ttcttttacc tatgaaatat
     8341 tttcacgtgt taaagtattc ctcgttgaac acaacaaata aaacttttta acgaataaag
     8401 gagatttttc atgacatcac ttaaaacttt actttttgga acaactttgg ctgccggcgc
     8461 tgcattcttc atgggaacga ctgctcatgc ggacgaagct tataccgttc aatcaggtga
     8521 tactctatca acgatttctc aaaaatacgt tggtgataac tccttgatca atgccattgc
     8581 agaatcaaat tcaatttctg atattaacct gatctactca ggacaacaat taacaatccc
     8641 aacagaaggc tctgcacaag cagctgctga acctcaagca gccgttcaag aagcaccagt
     8701 ccaagctgaa cctgttcaag ctgaacaacc tgttgtacaa gaaacggttc aaacggaaac
     8761 acaagcagcg cctgttgctg aaacacaacc tgctcctgct gtaacagaaa ctgctgctac
     8821 tccagcaagt acaagctctg caaaagaatg gatcgcacaa aaagaatcaa gcggttctta
     8881 cacagcaacg aacggacgtt acatcggccg ttaccaatta gattcttctt acttaaatgg
     8941 tgactactct gctgcaaacc aagaaagagt agcagaacaa tatgttactt cacgttatgg
     9001 ttcttgggaa gcggcgaaag cattctggga agcaaacggt tggtactaag attcattatt
     9061 tgaacgttta aacacattca aaatatagta agaggtcatg cgatttattc tttcgcatga
     9121 cctctttttg agtttaagtt tagtctttat tccttaacta tcgtttgcca gtatttcttg
     9181 tatcagaatc tataaatcta ctatttgaat caatctatta ggttttaaac tgtcccacct
     9241 tcacacaaat tttttttgat ttatttgaat ctggttccgt tgtttaaaat ttttcctata
     9301 ttcttttaga taatatgtta ttttctttct catcgctcca ggtgtacctt ctctttataa
     9361 aaaaacaaat tatgcggcta agaagactcg aacttccacg aggtcaccct cactagcgcc
     9421 tgaagctagc gcgtctgcca attccgccat agccgcatca catcttttag tttaggatta
     9481 catgtgtttt ttgtcaagaa catatttttg ttctttttca tttctgtacg ccctccctta
     9541 tgccatctac acaaataatt catccgagca actccatcat ctattgaggg ttggtgaaaa
     9601 aatctgtttt atccatatga tcgatttcaa tgttaaacga acgactctgg gattagtaac
     9661 tattttattt ttttcttcat ttcttttttc ctctttttct cgattcagct atcttctttc
     9721 atttggatat tgattatttc ttctgttgat tactatcctt ctggaattct agacgctgaa
     9781 gtaattcacg ttttctttct tctagagatc ataagtaacg aatgaacgaa cttcaaataa
     9841 tttttttatt gtaattcata tttgtattgt tcgatgattc ttaatacgat atgacacaat
     9901 aaataaaaat aggcatggct atttacaaca atagtgctat agtagaaaac aaatgaataa
     9961 ggttacttaa agaggtgaat acgatgaaac tgatcaaact atgggaagca gatttaaaga
    10021 aagcttatca gctacaaact agctttggac caaatgagag tggatttatc aacaatgctt
    10081 atggctatac atttgctgaa tttttagatt atgtaaaaaa atgcgaaaaa acgtctttag
    10141 gaatcgatct tcctgagagt tacgttcccg caaccgtctt tattttagaa agtgatgaac
    10201 atgagtatgt aggaattttt aatttgcgcc ataaactgaa cgaaacatta agaaacggcg
    10261 ctggccatat tggttacggc atctccccta attatcgtaa aaaaggatat gccacacaag
    10321 gattagcatt agtactacaa gaggcaaaaa aaatcggcat tgatgaggca cttttgtctg
    10381 taaataaaaa aaatcttggc agtcttaaag cacaacaaaa aaatggggct gcgatttatc
    10441 gtgaagatga gaaagaatac tatacgaaaa tcaaactgat gtaaaaaaga aggattccaa
    10501 aaggaaaggg atttgaatgt cctttatcaa tagaacacat ttatttaatt aacttataat
    10561 atagcagaca aaattttata aacaaagaaa ccttttatct cttttcttct agaaaggata
    10621 aaaggctttt ttgtttttcc tatttgcttc tcatagctag tcagccaaga tttctatcgt
    10681 atccccaacg caatccgtac ataacggctc atcttatctg tcgtccatgc tggataccag
    10741 actagtttga cttctgcttt cgttacttct ggcacttctt ttaatgcgtc atggatagat
    10801 tctgttaaaa tatctgctaa tggacagccc atagttgtta gagtcatctt aatgactgtt
    10861 tctccatttt caggattgaa ttctatatca taaatcaagc ctaaattgac gatgtcgatt
    10921 cctaattcag gatcaatcac catttccaag gcagcaagaa tacgttcttt gattagttct
    10981 atttcttgtc cagaccgttc ttggtttgct tcgctcatcg tacgacactc cttaacacga
    11041 tttttattta tcatactctt tttttagctg cttgtaaatg agaatttaga agtaagattc
    11101 tcgtttaaat ttataaaaat gttcttatta gatgtaatag attaaattta ttctaaaata
    11161 aacctgattc ctcatttttt ttgtgaacga ttttttattt ctggcaatac gccagttgtt
    11221 cgatacattt tttgttcgtt ttcatttgca aaccggccaa atactgaatg agttggtaaa
    11281 atattgataa atgcatcgtc gtcgatttca tgaacgatct gatcaagatc atacatttcg
    11341 tagcgggtga tgaccatcat gatcatgttc ccttgttttt ttgaatagcc accgacagac
    11401 ggcagtaatg tcattccacg taccatccgt tttgctagtt cttctgttac ctcttctggc
    11461 ttgacggtaa cgatcattgc tgttactttt tgatgactcg tgtggatggc atcaattact
    11521 tgagtcattg cataaatgga aataatcgta tacaaggcac tttcccagtt aaagaagaag
    11581 cctgcgatga ctacaatgat accatttaga atcagcatgt agtttccgac tgtttttcca
    11641 gttgtttgtg aaagtacaag agagatgata tccatcccac cagttgtaaa gcccattttt
    11701 aaagataatc ctacgccaag acctaataaa acaccgccta ctagggcatt catcaaaata
    11761 ttatcagtga tctgtccgac tggaagcagc atcgtcatca ccgatacaca tacaacattc
    11821 acaaaactaa ggatcgttgc tcgttttcct actttgagaa accctaatac aaacacaggg
    11881 atattcaaaa gaaaaataaa tgttcctgtg tctatcttga tatgaaacca ttcatataaa
    11941 agcgaggaaa tgatttgagc gatcccattc atccctgcag aaaagacatt ggctggaatc
    12001 aaaaaataat ttaaagcgac cgcagcggtc aatccggtta tcaaaataac cactattttc
    12061 tttaatcttt catttttatg atacgcagtc ataattttac tcacaagcac ctctccccta
    12121 ctttggtatt ccaaatgtaa gaataacagt ttatctatta aaataaaata cttcgaactc
    12181 aaatgagaag aattttagca aacaggtaag aatctgccct aatcctatga taagatagat
    12241 gtatggtaaa tatttatatg tatatcagga gggttcctat ggcaaaagat gctagcttcg
    12301 acattgtatc agaagtcaat acagaagaag taaaaaatgc gattcagcaa acaacaaaag
    12361 aattgtccaa tcgctttgac ttcaaggatt ctacagtaga aatcaaatta gagaacagca
    12421 atctagttgt attgagcgat gatgatttca aaatcgaaca gatcaaagat gtattatttg
    12481 gtaagctgat caaaagaaat gtgccaatca aaaatatcca tttttccgaa tcagaacacg
    12541 ctctaggtgg caaagctcga caaactgccg atttggtcaa cggaatcgat cgtgaaaacg
    12601 cgaagaaaat cactacagct atcaaaaatg aaaaactgaa agtaaaagca caaatccaag
    12661 aagatcagat tagagtgaca ggtaagaacc gtgatgatct acaagctgtg attgctttat
    12721 tacgtaaact tgacttacca atcgagttgc aattcactaa ctatcgttaa tcaaaaaacg
    12781 aggctgacgt ctgtcattcc tcgttttttt gatttactgc ttcttttgat aaccaaaaac
    12841 gctgtattcc ttcttccaat tccccaacac ttcgtacttc tcttgctgtt tgccaatgtt
    12901 gtgggaataa acgtcgataa gaaaaactag aaaatcgttg atctgccaat acaacgattc
    12961 cttgatccgc catgccacga atcactcgac caccagcttg caagactttg ttcattccag
    13021 gaagctgata agcgtaagca aatccaaggt gttccttctc atcataatat gatttgatca
    13081 gttcctgctc atgattcatc tgcggcaaac ctacacctac gatcatactg ccgatcaatc
    13141 ggtcccctct caaatcgatt ccttctgaaa aaatcccgcc aagtacgcaa aatccaatca
    13201 atgtttcagc gggatttgtt tgaaattttg ttaaaaatcg ttcgcgttct tcctcattca
    13261 tgtccgtttc ttggattagc acattgctgt cgggatattg ctgtctgaat gcttccaccg
    13321 caagatctaa atattgataa gaaggaaaga aaacgaaata attcccgacc tttccatcag
    13381 caaaagcatg aatcgtctct actaattttg ccagactgtg ttcccttttt cgataagtcg
    13441 tctctaggta atttgcgatg aaaacttgct ggtttccttc tgggaaagga ctcggcagtt
    13501 tgtaagccag acttttttgt ccccccagtg tttcctgata ataagagagt ggtgaaaaac
    13561 tagcagaaaa aagaatactg ctttttcctt tatccaacga ctgctcaaga aacaagcttg
    13621 gatcaataca aaactctttt actatcagat cgcgatacgt tttttcgact gttgtttcgt
    13681 agtgatcatc ataaaattca ctgaccttca aaaaatgaag caaatcaaaa taataaggaa
    13741 gcaattgttc ttgtgatggg tgctcaggaa attctgccag ccattcttta attttttcgc
    13801 ttaattgata acctgccttt actaaagatt cagccggtgc tttttgatga tgataatccc
    13861 aatgatcttc ttttgctatt tctcggatcg aatcaaattc ttttaatagt ttgttgaaac
    13921 ggcgatgaag ttttttgaat tcctttggta tagcttcctt acttcgtttc gttttttcat
    13981 aagatagttc tgccgaatac atctctcggg aacgattcac caaattatgt gcttcgtcaa
    14041 tcaaaaacag atagtcctcg tttttttcat cttcaaaaaa acgtcttaaa taaacagttg
    14101 gatcaaataa ataattgtaa tccccaatga ttacgtcaca ccagatactg acatctaatg
    14161 atagttcgaa aggacaaagt gtatgctttc ttgcataagt ttcgatgact tcacgagtga
    14221 tctgattctc atgattcaaa agatcccata aaccttcatt aatccgattg taataaccat
    14281 tggcatatgg gcactgatct ggattacagg tcgtctcatc taaaaagcag atcttgtctt
    14341 tcgccgtcaa agtgacactt tttacttcag ctcctgtatc ttttaatgct gtcatagcat
    14401 cttcagctac ttgtctagtt attgttttag ccgttagata aaagatcttt tctccctctt
    14461 cttcccctac agctttcaaa gcggggaata acgtagagat tgtttttcct gttccggtag
    14521 gtgcttctac aaacaatttc tgtttcgtac ggatcgtttt ataagcagct accgctagtt
    14581 cacgctgacc tttcctatac tcatcatggg ggaatttcaa agccatcaaa gaggcattgc
    14641 gtacagtgcg ccagttttct tgaaaaacaa gccattcttt ataatcttca gtcagctttt
    14701 taaaaaaagt ttctaactgc tctcttgtct gatgttcaac ctttctcgtg atcacttctt
    14761 ctgccgtctg gaagtaagtc aattgcagat ttatttcttg taaatcatgt tcttgactat
    14821 agatataggc atacaccctt gcttgatgaa agaaaagttc cacttgttct ggttctaaat
    14881 cttcaaatcg tggttcggag gttttgattt catctatata ataaataccg tctcttttga
    14941 aaataccgtc tgcacggcct tcaacgatca ggtaatattc atctaattga actgttgttt
    15001 ttaaaaagac ttctttttca tagttttctc ccgcatcttt ttggagcttg cggtggattc
    15061 tcgctccttc taatgcagta tggttacttt ttttacgatt gtcgatactt ccacgtcgca
    15121 aaataaattc tactaagtgc cgcacagata tttttgactg tttgatcatt tccaccacct
    15181 ctttctctct aagattacct gaaaaaacct agtttagcaa aaagagctct ctttccaaac
    15241 ataaaaaaac accctgctga ttaggcaggg atataggata tagaatttgg atttttggat
    15301 gaatctaatg taacaaggac atattaaaat gctatgaaaa cttactttcc ttctctttag
    15361 aatatccaaa tacttttcat atagtctgat aattcagata agtaaagaga aataagtacc
    15421 tgtttttgtt tcctagcaat gaatgcgtta tactaatgga tgaaagaagt tttggctatc
    15481 aaggaggaat gcacatgcat atcgaaagat taaaaacagg aatgattgaa gaaaactgtt
    15541 atttggtcta caacgaagaa gcacttttga tcattgaccc tggagaagat gcggaacgaa
    15601 tcaaacaaga aatcaaaaaa acaaaccaac agccggtagc gattttgctt acccatactc
    15661 actacgatca tatcggtgca gtggaacctt tacgcgcgta ttatcaaatc ccagtttatg
    15721 tcagcccact agagcaaaaa tggctaggtg atccgatttt gaatctttct ggactcggta
    15781 gacatgatga tattgctgat gttattgtct cgcctgcaga atatgaattt gaaatgaaaa
    15841 cttatacttt aggaaacatg tcttttcgcg tcgtccctac cccagggcat tctataggaa
    15901 gcgtcagctt tatttttgat gattttgtcg tttctggcga cgccctgttt agaggaagta
    15961 tcggtagaac cgatctgtat acagggaatt tagaacaatt acttcatagt attcggaccc
    16021 agctgtttgt attacctgat gaatttgcag tctatccagg acatggcgat gcaacgacga
    16081 ttgaacagga aaaaaggaca aatccttttt tcaatcgtta aaacgactta ggaggagaat
    16141 aaaatgacag aaactacact ttatatcgta cgacacggaa aaacgatgtt caatacaatt
    16201 gaacgagtac aaggctggtg tgatacaccc ctcacaaagc aaggacaaga aggcattcat
    16261 tatttaggtc gcgggttaaa aaatgtcgac ttttcatttg cttattctag tgacagtggt
    16321 cgtgcaatcg aaactgcccg tattattcta ggagaacatt ctaagggaaa agaaattcct
    16381 tattttattg aaaaaaggat tagagaatgg tgtttcgggt ctcttgaagg tggatatgac
    16441 atggaaatgt ggggtgtgat ccctcgtgta ttgaatttta aaacatatga tgaaatgttt
    16501 accacagaag tttcttttga acaaattgct aatgctgtat acaacttaga taccgctggg
    16561 tgggcggaaa cttatgaaac attgcgagac cgtgtatgga caggatttga agatatcgcc
    16621 catcactgcg aaaaaaatgg tggtggaaat gttttagtcg tatctcatgg tttgacgatc
    16681 gcctttttgc ttaatctgat cgatcctaaa cagcctgttc gtgctgggct tttaaatggc
    16741 agcgtaacaa aggtcactta tgcaaacggt cgattttcta tccacggcat caatgatacg
    16801 tcttatatcg ataacgggaa aacacatagt cgcgactcgt ttctataatg gggatatttt
    16861 aatttttacc agagaatcat aaaaggcaaa gtctgaacga gggctttgtc ttcttttttc
    16921 tcttcgtgta cttatctgct ttatttctca agtttttctt aatattaaaa aacagattgg
    16981 gtttctcaaa aaataacggt atactataag atgtatctta attgggaagg aaggaattta
    17041 tgttttttgc aaatattatc aaagcagtga ttctcggtat tattgaagga ataacggaat
    17101 ggctgcccat cagtagtact ggtcatttga tcctagcaga tgaattcatc aaactggatg
    17161 ccagttcaca atttatgtcg atgttcaatg tcgttatcca attaggggcg atcttggctg
    17221 tcgtcgttct ttattttcac aagttaaacc cattttctcc aagtaaatca acggtagaaa
    17281 aaaaagatac gtggacactt tggtttaaag tgatcgttgc ttgcatacca gccatcatca
    17341 ttggtttgcc tttggatgat tggttagaag caaatttcca caagttctta ccagttgcca
    17401 ttgttttgat cgtttacggt attgccttta ttattgtcga aaaacgcaac aaaaacaaaa
    17461 cacctcgttg gacttctctg aatgacttta cgtaccaagc agctcttctt gttgggttat
    17521 tccaagtctt ggcacttgtt cccggaacat cccgttctgg cgcaacgatc ctaggatcta
    17581 tcttgattgg tgcttcgcgt tttgtcgcta cagagttttc atttttctta gggattcctg
    17641 tcatgtttgg cgcaagcggc ttgaagatcg tcaaatattt agctgaaggg aattcattca
    17701 aaatggaaga aactgttgtt cttcttgtag gaacattcgt atcttttgtg gtttccgtct
    17761 tagctatcaa attcttacta agctatttga agaaaaacga ctttaccgct tttggctggt
    17821 atcgtattat ccttggtatc attttgattg gttattggtt tattgcaatg tagaaaaaac
    17881 cagaacactt ctcttatttg agaaatgttc tggtttttta ttttctttaa ta
//
LOCUS       DACDEG010000047        17319 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.049, whole genome shotgun sequence.
ACCESSION   DACDEG010000047 DACDEG010000000
VERSION     DACDEG010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.049"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(47..358)
                     /locus_tag="IV142_001661"
     CDS             complement(47..358)
                     /locus_tag="IV142_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP8485323.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(360..557)
                     /locus_tag="IV142_001662"
     CDS             complement(360..557)
                     /locus_tag="IV142_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP8485324.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(951..2678)
                     /locus_tag="IV142_001663"
     CDS             complement(951..2678)
                     /locus_tag="IV142_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8485325.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2671..3864)
                     /locus_tag="IV142_001664"
     CDS             complement(2671..3864)
                     /locus_tag="IV142_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8485326.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4051..4695)
                     /locus_tag="IV142_001665"
     CDS             complement(4051..4695)
                     /locus_tag="IV142_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8485327.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4789..4923)
                     /locus_tag="IV142_001666"
     CDS             complement(4789..4923)
                     /locus_tag="IV142_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP8485328.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4925..7057)
                     /gene="feoB"
                     /locus_tag="IV142_001667"
     CDS             complement(4925..7057)
                     /gene="feoB"
                     /locus_tag="IV142_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP8485329.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7054..7527)
                     /locus_tag="IV142_001668"
     CDS             complement(7054..7527)
                     /locus_tag="IV142_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP8485330.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7928..8152
                     /gene="nrdH"
                     /locus_tag="IV142_001669"
     CDS             7928..8152
                     /gene="nrdH"
                     /locus_tag="IV142_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP8485331.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8311..10470
                     /gene="nrdE"
                     /locus_tag="IV142_001670"
     CDS             8311..10470
                     /gene="nrdE"
                     /locus_tag="IV142_001670"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP8485332.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10520..11485
                     /gene="nrdF"
                     /locus_tag="IV142_001671"
     CDS             10520..11485
                     /gene="nrdF"
                     /locus_tag="IV142_001671"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP8485333.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11574..12713)
                     /locus_tag="IV142_001672"
     CDS             complement(11574..12713)
                     /locus_tag="IV142_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8485334.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13022..13735)
                     /locus_tag="IV142_001673"
     CDS             complement(13022..13735)
                     /locus_tag="IV142_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP8485335.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13989..14690)
                     /gene="nagB"
                     /locus_tag="IV142_001674"
     CDS             complement(13989..14690)
                     /gene="nagB"
                     /locus_tag="IV142_001674"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP8485336.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14924..16456
                     /locus_tag="IV142_001675"
     CDS             14924..16456
                     /locus_tag="IV142_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8485337.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16647..16826)
                     /locus_tag="IV142_001676"
                     /pseudo
     CDS             complement(16647..16826)
                     /locus_tag="IV142_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(16846..>17319)
                     /locus_tag="IV142_001677"
     CDS             complement(16846..>17319)
                     /locus_tag="IV142_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HAP8485338.1"
                     /translation="VGYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHDVKVLET
                     LSEEANLPNILGDKGYISHKIHEKLALKGITISVPPRKNMDKSEKLDHSLLGKQRKTV
                     ETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         5375 a         3546 c         2980 g         5418 t
ORIGIN      
        1 tttctaatca aaaaattttg agacatattt aatattataa gtatcattaa aattgagatt
       61 tatctccata atctgctcga tataaaccat ttattccagc agttcttcca atttcactca
      121 aatttcttat taattcctct tgatattcag tgaatcttat tttattagta taagcagtaa
      181 aatatagata attcaccaat ctatttatta aaggactggg atctaattca tatttttgtt
      241 tttctaattt taaatatacc ttttgtaaaa cctcaagaat atcagaatat ttaggatctt
      301 tacttataga tatatcatta tataattcat gaacaatttg cttagcattt tttttcattt
      361 tagcacttcc ctggaattgc tccacctaaa aaaccaccta tagacattcc tgcaatacaa
      421 gtagttgcct tggcccaatc gaccgtacat ttatttttag tgcaatacac tccatttcca
      481 taatattttc cactatgagt ggtaccccca tagataagtt gtgtctcttt aatagacaaa
      541 atttttaaat gtttcataat ctaatcaccc ctaaattaat gtttttagac tattattcta
      601 acattcatta aatttcaaaa aacaattcct tcttgaaacg tagcaaaata attcctgaat
      661 aagaatttaa tcacttttaa gtatataaac tatgtatatt ctttaatatt ttttgagaat
      721 gcacttcgta cataataaac tttaatcaca ttagtctttc atacctacga tcgatctctt
      781 tgactcgcac ccataaattc ataatagttt gtcggcgtct ttgaacgttt cgaagatgag
      841 ctacgcattc tcttttttta tagacaaaaa agatagagac gaacatggag cttccaaatt
      901 tggatatttg atccaatttt ggaagctcca tttatttcgt gtctgtcttt ttattttttc
      961 cagctttcga ctaattcttg gttctctttg atccagtctt ttgctgcttg ctgtggatct
     1021 tttccattgt tgatttccaa cataactttt tccatatctt tttctgacca atggaaattg
     1081 tctaatactt tgtaagcttc tggctggtct tctttcaatc cttttcgaac catcgtatgg
     1141 atctgctctt cgcttcccat tccgttttca ggatcttcta agtatttcag atcatatttg
     1201 gcaaacatcc aatgaggtgt ccatcctgta actacaattg gttcgtgttt tttgatcgct
     1261 tgacctagtg caacggtcat tgctcctgaa gaagatgtct ctactttcca gtctttcaag
     1321 ttgtcatatt tttggatcgt gttttcagca gccgtaacta caccagctcc tggctcgatt
     1381 cctatgattt tcttacctgc ctgatcggtt aactcatcga tcgaatttac atccatgtag
     1441 gctggcactg ctaaaccaac ttttgctcct gtcaaattgg cacctaggtc ttcgacttga
     1501 tctttatatt gtgcatattg tgaaccatga gttttaggta gccaagctga aaccatggca
     1561 tctgactcgc catttgaaac agatttccac atgattgaat tatctaatgg cgtcatcgtt
     1621 acatcatagc ccatttgttt caatacttca ccgactacat ttgttgaagc aacttctgta
     1681 tcccattcaa cataagataa gttgatacgt tttgtttctt tggcagatga aaaactccct
     1741 ataccaatcg cacctaagat cactactact acagcaccaa tgatcagtcc ttgacgtttt
     1801 ttacttttct gtgttgttgc tcctgcagct tttttattgt ttggctggtt caaatgctgt
     1861 gtaaaacggt caatgatgat tgctaagata actaatgcaa ccccgttgac aaatccattc
     1921 ccgacttgtg ctctttgcaa tgctgataat acgccgcgtc ctaagccggg tgcaccgatc
     1981 atagaggcaa taacgaccat tgaaagtgac agcattgttg tttgattgat ccctgccatg
     2041 atcgtacttt tagctaaagg caattctagt ttgaatagtt tttgacgact tgtgctcccg
     2101 aatgaatcag aagcttctac taattcttta ggaacttgtc gaatccctaa attcgtaaaa
     2161 cgaacggttg gtggcagagc aaagatgact gaggcaaata cccctggaac catcccgata
     2221 ccgaaaaatg caactgctgg aatcaaataa acgaatcctg gcattgtctg cataaagtca
     2281 aggatcggtt tgataatact ttgggcttta ctgctttttg ccatcaagat ccctaatggt
     2341 accccaatga tgatcgagat cacactagat aaaagaacaa gtgtgacagt attcatcaaa
     2401 tcattccata gaccttggtt ataaataaac catagaccga ttaatgtgaa caacgtaagt
     2461 cctggacgtt tcttagaaat aaagaaagct gctaccgtca ataacaagat aaacaaagga
     2521 gctggaatca ctaacagtaa atttgtaatc cccgacatca ctgcctgtcc aatcgtttgt
     2581 aaaaatgaaa ataggccaga aaaagttgtc gtaaaccatt ctgtgatatt ttctacccag
     2641 tttgccactg gtaattgata gttcaataga ttattcatgt tcactcacct cgcttgtttc
     2701 tgctaatgct tcaatcacac tacctcggat gacgacacct tttaattttc catcctctac
     2761 aactgcaagt ggagtcggtg agtcataaat aatattgaaa atatcactta ccaccatgtc
     2821 tttcccaatc accgtcacat tttcatctag gaagtcgatc aatggacgtt tttgcttcct
     2881 agcttctaac gcttgatctg cagtgatgat ccctttcagg tgtctctttc tgtcaacagc
     2941 catcagcatg ctgacttctt cattacgcat ccttgtcaat gcaacgtttg gtccatcaga
     3001 ttcgatattc gtcgtcaatg caggtaccat gatgttttga gccgtaagca ctttggaacg
     3061 atcgacttct tccacaaatt cacgtacata atcatttgct ggattggtca atatttcttc
     3121 acctgttccg atctgcatga tttcgccgtc tttcatcaag gcgattctgt ccccaatccg
     3181 taaagcttca ttcaaatcat gggtaatgaa aataatcgtt ttttgaacat tggcttgcag
     3241 gtccagtaat tcgtcttgca tttctcgccg gatcagcgga tcaagagccg agaacgcttc
     3301 gtccatcagc aagatctcag gatcatttgc taaagctctg gctaggccaa cccgttgttg
     3361 catcccacca gataactgac taggatattg gtctttaaat gaaagcaaac tagaattttc
     3421 taatgctttt tctgcttttt cttgtcgttc ttctttaggt acaccacgga cttctaaccc
     3481 gtattctgta ttttccaaga tcgttcgatg tggaaacaga ccgaagtttt ggaaaaccat
     3541 attgatttta tgacgacgga cttctcgcaa ttcttctttg cttaacttcg atacatcttc
     3601 tccatcaata tagatactgc ccgaagttgg ctcaatcaga cgattcaata gacggatcaa
     3661 tgtggatttc ccactcccgg aaagccccat gatcacgaag atttctcctt cttttacgtc
     3721 aaaattcacg tcatacacac caaccgttgc accggttttc tttaaaatct ccgttttcgg
     3781 ctggtgatca ttcatcatat ctaaagcggc ttgtgtcttt ttaccaaata tctttgttaa
     3841 atgttttact tctactttta ccaaacaaat tctccttttt tatgtaaatt catttttctt
     3901 tctgaaaaaa gtgaccacac tatgttaaca tcatgtagtc actttgtcaa aaatgaaaga
     3961 aatttgcttg tccaaactga aaaaaacttg gctaaatctt tgtaaataaa aggtttagcc
     4021 aagttaatat ttttttaata tttcacacaa tcattctgca agctgatgct ccgggtaaaa
     4081 aaagtttgtc atacgctgta gtgtatattc atttccaata aaatgaacga tgtcccctgc
     4141 cgtaaattta gcataggggc caggtgaaac aagcagttct tcattatgag aaattgcgat
     4201 aactgtggcg ccagtcgcct gccagatatt caactcgcta attgaacggt ccagatgcat
     4261 tgctttttct gataaagata attcaaacgg aagtaaagga ttcatcttat gtattcgctt
     4321 cgtttgattg accagctgat ccaataattg agaaaaatga ttcagctcat cttgttgctt
     4381 ctccacactt gccatgattt ctgcttttgt atcttggatg gattgtacat cttgatattg
     4441 caataggaaa tcttttgctt tttccttcga agcaacaaat gccccacttc catgccgtac
     4501 ttccatgatt ccaagatcta ccagcacatt gatagccttg cgggtagtct caggagaagc
     4561 attgtacgta ctggcaagtg ttgagcgggc atggatcttc tcacccactt tatatcggtt
     4621 ttcaacgata cgttcggcta aatcaacagc aatctgctgg tagcgcgggc ggacgatttt
     4681 tttctttcca gacattttct ttcctctttc tttttttact ttccctcatc ttactatgct
     4741 ctagcaaaat aagcaaaaaa ggggagaata gcctcttgca acgactgatc atcgcttgat
     4801 ctgttccttt tttactgggc aagctgtttg acagtcatca caattcttac cggattttaa
     4861 acgagtatag acaattgcgc ctgcacttcc aaagattaac acacttaata agaatgtcgc
     4921 catcctattc ctctccttct aatgacaata tagtataaaa ctggttcttg gattttggct
     4981 gacggaacaa aacataaacg agtcctatca tcagtaaaat agcaagatac gtccctaacg
     5041 ctacctgtcc accctccaat aagtgtccca actgataaat cacaaaacta actaaataag
     5101 ctaaaccgca ttgatagcct atggcgatcc aagtccattt cttggcattc atttcccgat
     5161 ggatagcccc aattgccgca aaacaaggcg cacaaagtaa attgaaggct aacaatgaat
     5221 aagctgctgc gggagtatat gtgtgttgta gcacttgcca gatttctctg ccattttcgg
     5281 atacttccgc atgaccaaac aaaataccaa atgtgccaat aatattttct ttagcgacca
     5341 atcctgtaat cgctgcgact gttccttgcc agtttcccca accaagcggc tgaaaaacaa
     5401 ctgccaggat tcgcccgaaa aaggctaaga tactttggtc ttcagaaaca ggttgagcat
     5461 aaaaattata agaagagctg aaccagatca cgatactaga aacaaagata atcgttcctg
     5521 cttttttaac aaaagacttg ctgcggtcat aggtttgtct taggaccgct gaccattgag
     5581 gaaaatgata tgcgggcaat tccatgataa aaggagctgt atctccagca aacaaacgtg
     5641 tttttttcaa gctgattcca gagaaaataa ttgcagtcat accaagaaaa taggcagaag
     5701 gagctaccca agattgatga ggaaaaaagg cccctgctac taaggcaata atcggcaact
     5761 ttgcagaaca aggcatgaag gttgtgacca tgatcgtcaa acgacgatct tgctcgtttt
     5821 caatcgtgcg actggccatg acgcctggca ctccacatcc tgtagcaatc agcataggaa
     5881 tgaaggattt tcctgaaaga ccgaatttcc tgaaaagtcg atccatgaca aaagcgatcc
     5941 tcgccatata tccgcagtct tccagaaagc tcaagcataa aaacaatata atcaattgcg
     6001 gtagaaatcc taacactgct cccacgcctg cgatgatgcc gttcaagatc agctcctgca
     6061 tccatcccgc tacttgccat tcagctaaca atctgccaac atgttctggt aaccatgtac
     6121 caaacaatgt gtcattgatc caatctgtcc ccattgttcc tactgtttga atagccagat
     6181 aatagatcag ccacatcaca aatgcgaaga tcggcaaggc taaccaacga ttcgttgcaa
     6241 tctgatcgat tttgtcactc atcgataatt ggaaagaatc atttgacaca acacagagtg
     6301 tacatagttg ggtaataaat tcataacgtt cattgaccag aatcgtctca ctatcgtcgt
     6361 ccaatagttt ctctgttaac tggatcagtt gctcgatctc tttttgctga gaaactgcta
     6421 tctcgtaatc ctctcttacc ttctgatctc gttcaaaata tttcattgca taccagcgtt
     6481 tcttgccgct ttgttctcct agtacatctt caatttcatg taaagctgct tctaaacgtg
     6541 gatcatatgt acagggaaca ggaatagaag gccgcttttt tgcagactcc tgaatctttt
     6601 cgattagttt ttcgattcct tgctttttca atacgctgat tggtaaaact ggtacaccaa
     6661 gaccatagga aagcttttct agattgattt tttttccttc ttttttgatg acatccatca
     6721 tattcagacc aatgatcata ggaatgtcta aatccaataa ctgcgtagtt aaataaagat
     6781 ttcgttctaa attggtagca tcaataatgt tgatgatcag ttcaggctgt tcctgaacca
     6841 aataatctct tgcaatcact tcttctggtg tataaggaga caaagaatag atgccgggta
     6901 ggtcctgaat atgaatgttc ggattttttt tcaacgatcc ttcttttttt tctactgtta
     6961 cgcccggcca attccctaca tattgattcg aaccagtcaa gagattgaac aagctagttt
     7021 tcccgctgtt tggatttccg actaacgcga tattcattct ttttcctccc tttgtatcaa
     7081 gattagttcg gcctctgact ttcgtaacgt cagttcatac cctcgcactg tcagttcgat
     7141 tggatctccc agaggggcta gttttcggat aactattttt acatttcgcg tcaatcccat
     7201 atccattaac cgtcgcttca ctgcgccttg tccgtggatt gctacgacga tcccagtctc
     7261 tcctgctttt aattgatcaa gagaagtcca gctttcagag gaagcagtct ttttattgac
     7321 aaagatttga cctagtattt cccgacttac tgccactcga tgacttttta ccagtaaaat
     7381 ggcattgttg cctgataaat tgacgacgac gattttactt ccggcaacga tcccgatatt
     7441 ttgcaaatgg cgttttgttt tttcttctgt ttcgattcgt tcgacttgat aaacgaaatt
     7501 ctgctttgcc tgcgctaagg tctgcatatt gattcctctc ttgtgaaata aatcatgtat
     7561 taaaatcatt cattatatgt tctaattttc ttacgtgtta cgcaaataaa tgagaatcat
     7621 tctcacatag aatactctcg gtaattgaga atgtcaatta tacttgtgat attatgagcg
     7681 aatctttagt attgatagat aaaaatgaga aaaaaatatt tcttcatagt tttccacaat
     7741 tttttctcta ttaaatttgc tattttccca cacgatactt gttttcagct cttttttcaa
     7801 aaacacaaaa cttagtattc cacagtttcc acaacacaat atgtagtgtt tgttgtttga
     7861 cttttttact aagagtttta tactatctat tatagataat aagaatgtgt tagaggaggc
     7921 actcaccatg aacgtaaaaa tcttttctaa aaataattgt atgcaatgta agatggtcaa
     7981 acggttttta acagaaaatc aaattgcttt tgaagaagtc aatatcgatg aacagcctga
     8041 agcagtagac tggttgaaag cacaaggatt ccaaagtgtt cctgtcatta cttctgatgc
     8101 agaaacagtt atcggattcc gtcctgatca gttgcgtaaa cttgccagct aacatgacta
     8161 aaagaatcaa aacgtgatac atggatttga agggcttgag acagcaccag tttttgtcgc
     8221 aaacttccaa atcctttttt aaagcttaaa aacaatgcaa tgatttgcaa tcaagcatca
     8281 tcaaatacga aatgaaggtt gggcgtacgt atgagtctta aaaatttaca agatgtcagt
     8341 tatttcaagc tgaataatga aatcaatcgg ccagtcaatg gtcaaatccc tttgaataag
     8401 gataaagaag ctttagttgc cttttttgag gaaaatgtca aaccaaatac gatgactttt
     8461 ccatctatta tggaaaaaat caattacttg atcgatcaag aatatataga agcagaattc
     8521 attcaattgt attcaccatc atttattgaa aagctatatc aatttttgga cgagcaacat
     8581 ttttctttca aatcttttat ggctgcttac aaattttatt ctcaatatgc gttgaaaacc
     8641 aacgacggtt ccgcttatct ggaaacttat gaagatcgag tagccttcaa tgcgctttac
     8701 tttgcgaacg gggatgaaga attagcactt gctcttgcag acgaaatgat ccatcaacga
     8761 tatcagccag ctacaccttc tttcttgaat gctggacgta aacgtcgggg cgaattggtg
     8821 tcttgtttct tgactcaagt cactgacgat atgaactcta tcgggcgctc aatcaactct
     8881 gctttgcaat tgtcccgtat tggtggcggc gtagggatta ctttatcaaa tcttcgtgaa
     8941 gccggcgctc ctatcaaagg atatgagggt gctgcaagtg gtgtcgttcc agtcatgaaa
     9001 ttattcgaag atagtttcag ctattctaat caattaggtc agcgtcaagg agctggcgtg
     9061 gtttatttga acgttttcca tccagacatt attgctttcc tttcagcaaa aaaagaaaat
     9121 gcagatgaaa agattcgcgt aaaaactctt tctcttggcg tgatcgtacc tgataaattt
     9181 tatgaattga cacgtaaaaa cgaagatatg tatttattta gtccatatag tgtcgaaaaa
     9241 gaatatggtg tcccattctc ttatgtaaat attacagctg agtatgatca tctagttgca
     9301 aatccaaaca ttcgcaaaca aaaaatcaaa gcgcgtgatc ttgaaaatga aatctcaaaa
     9361 ttacaacaag aatctggcta tccttacgta atcaatattg atacagcgaa ccgcgagaat
     9421 ccaatcgatg gcaagatcat catgagtaat ttatgttcag agattttaca agttcaaacg
     9481 ccttctgtga ttaacggcaa acaggaatat gaagtattag gaacagatat ctcatgtaac
     9541 ttaggctcga caaatatcgt gaatttgatg caaagcccta actttggtca atctgtgcgt
     9601 acgatgactc gtgcattgac atttattaca gatgcatctg atatcgatgt cgtaccaccg
     9661 atccaaaacg gaaataagct gaatcatacg attggattag gtgccatggg cttacacact
     9721 tatcttgcaa aagaacaaat ggaatatggc tcagaggatt cattagactt tacaaatatt
     9781 tacttcatgc tgttgaatta ttggacactt gttgaaagca acaatatcgc tcgtgaaaga
     9841 aaacaagttt tccataattt tgaaaaatca gcttatgctg acggcactta tttcgaaaaa
     9901 tacgtaactg gagaattcca gccaaaatca gaaaaagtca aagcattgtt tgaaggcatt
     9961 tttatcccaa ctgctgaaga ttggaatgct ttgaaacaag cagtaatgaa agatggactg
    10021 tatcatcaaa atcgcttggc agtagcacca aatggctcaa tttcatatat caacgatacg
    10081 agtgcaagta ttcatcctat cactcgcatg atcgaagaac gccaagagaa gaaaatcggt
    10141 aaaatttatt acccagcgcc ttatctatcc aatgagacga ttccgtatta cacttctgct
    10201 tatgatatgg acatgcgtaa agtaatcgat gtttatgcaa cggcacaaca acacgtcgat
    10261 caaggcatga gtttgacttt attcatgcgt tctgaaatcc cagaaggcat ttatgaatgg
    10321 aagacaacaa cgaagcaaac gacacgtgat ttgaatattt taagacatta cgctttccac
    10381 aaaggaatca aatccattta ttacgtacgt acctttactg atgatgcaga agaaatcgga
    10441 agcaatcaat gtgaaagctg cgtgatctaa tatcatagat agattcgctt attcatattt
    10501 taattaaagg agcattctca tgtcagaaac atattatgaa gcaataaact ggaatgaaat
    10561 tgaagatatc attgataaat ccacttggga gaagctgaca gagcaatttt ggctagatac
    10621 acgtatccca ctttcaaatg acctagatga ttggcgtaca ttgtctcaat tggaaaaaga
    10681 tacagtagga catgtatttg gcggtttgac actgctagat actgtccaat ctgaaagtgg
    10741 catggatcag ttgcgtaaag atgtccgcac tccccatgaa gaagccgttt tgaacaatat
    10801 tcaattcatg gaatccgttc atgccaaaag ttattcttct atttttagta cactgaatac
    10861 gaaaaaagaa atcgaagaaa tttttgactg gacaaatacg aatccttatc tgcaaaaaaa
    10921 agctgagcgt atcaatgaaa tctacaaaca cggtacaccg ctagaaaaga aaattgccag
    10981 cgtcttttta gaaacattct tgttctattc aggtttttat actcctcttt attatttagg
    11041 aaacaacaaa ttggctaacg tagcagagat catcaaacta atcatccgag atgaatctgt
    11101 acatggaacc tacatcggtt acaaattcca attaggattc aacgagttac ctgaagcaga
    11161 acaagaaacg ttaaaagact ggatgtacaa tcttctttat gagctttacg aaaacgaaga
    11221 acgttataca gaagaattat acgatccaat cggctggaca gaagaagtca agacattcct
    11281 acgctataat gccaacaaag cattgatgaa cctagggatg gatccactct ttccagatac
    11341 tgccaatgat gtcaacccaa tcgtaatgaa cgggatttca acaggcacaa gcaaccatga
    11401 cttcttctca caagtcggaa acggctattt gttaggtcat gtagaagcga tgaaagacga
    11461 cgattatttg attggattgg aataaggatt tattttaatt caaaaatttt tcgaaaaata
    11521 aaaagagttt agcgtacaga tattttaaat gtacactgaa ctctttttta tgcttattca
    11581 cttgtttctt ccagcacttt tggactgtct aacttttccg aaacatgttc cattcttctc
    11641 aataaataga ttttccggat atttaatatt atcgtcttaa tcactgcata gacaggtatg
    11701 caaatcagca tgcctaatat tcctgcgaca cttcctgctc caatcaatac caagatgatc
    11761 gtcaaaggat ggatattcat tgatttccca aataacaacg gcttgatcaa atttccatct
    11821 aattgttgga tcactaaaat cgacaacgcc atatacaaag cttgaaatgg agagataaac
    11881 aaaccgacaa tgattgctgg taccgctcca ataaaagggc cgacatacgg gataatattc
    11941 gtgattccgc aaatgaccgc aagcaacaag ctataaggct gatcgaatat tctcattgcc
    12001 aaaaagctca ttacaccaat aatcaaagca tctaagatcg tactgctgat ataggcagat
    12061 aatgtttcgt ttaattcttt taccgtttgg cgtaactctt tacgaatcgc tttcggaaag
    12121 aagtgagaca acgcatcaat aaatttgtgc ccatccttga acataaatag caaaataaat
    12181 ggaacggtaa ataacaaaat aaaaaattgc atcataaatg aaatgatttt tgagacactt
    12241 gcagataaac tgactaatat gatattgaac aaattactaa gagataaatt tgctgatgct
    12301 aattgttctt ccaaattgaa atttttaaac tcttcccgct gcgccaattc atttaaccat
    12361 ttgcttgttt catctgcata cattggcaag ctctgggtca attgaattgt ctgttccatt
    12421 aactgaggca ctacattcat gacagttaaa acaactaaaa tgattacgac caaaaacgtc
    12481 aataaaaaac cgaacactct cgggatccga tgcttttcga gaaaaacaac gattggatcg
    12541 aacatataat agataaaacc agcaacaagc aatggtaaaa aaattgccga aagcattttc
    12601 aatattggac taaaaatatt cggcatttgt aaaataaagt acacgccgat gatcgtgagt
    12661 actagctcca ctgtccagaa aaataatttt gattgtttaa aacgtgattg catacatctc
    12721 ctccaaggtc ctttgatact tctattctat catttaacaa agagttacgc gctgagattt
    12781 tcatttgctt aaaaaaaaca aaaaaaataa acatatttta atgagtagac gagtacttaa
    12841 acaaaaacac ggtttggagt agtgacaaaa attatactgc agtccctttt tccaaataaa
    12901 cggtgttcaa aagcttgcat cttcgtaata agcccagcca aacgaaaaat ataaggaact
    12961 atttttattt ggctgggctt attacttgat gtaggacatc tattttacaa cctcagatca
    13021 tttattgcgc aatactgact aaatgcaact ttgtgatatt tttgtgtacg gacaactcct
    13081 cgatcacatc ggcataagtg agcccttttg gaagatcaat cgtatagata tttttgtaca
    13141 ctcggtagtc atcaacgaat tttacgtcaa aattcacatc ttcgattacg atatttttct
    13201 cttcaaaata atcggtaaga aaatctttgg tttcttttct atggataaat tggatttcta
    13261 actttttagt cgtaggtaca tggatcacgc gtttcactac tgtgagtgca atagcaatcc
    13321 cgataaaact gatgatcgca atggaatagt aacccatgcc gatggcgatc cctaatccag
    13381 caattgccca taaagaagcg gcagtcgtaa gtcccgtcac cgattgtttc gtgacgatga
    13441 tcgttcccgc gcctaagaaa ccgacaccac tcaccacttg agcaatcagc cttgcttcat
    13501 ctgaacgaat cgttccagca agctgtggat actctaaagc atctcttaat gcaccactag
    13561 cgatctcaac ttggatcaat gcaatgatac atgctcccat acaaactaaa atatgcgtac
    13621 gcatacctgc tggacgattt ttatattgtc gttcaaaacc gattgctccg ccaaatagca
    13681 tagcgatccc taaacgtaaa ataatctctg gaattgtcaa taccatatca ttcatctaaa
    13741 caccctccag tccaatatat catgtctgaa aggagcagac aaaaaaagtg caatttttac
    13801 ctcttacgaa aaaaggactg tgacaaaatt tttgtcatag tcctttttat gaataaacgg
    13861 tgttcaatca gctgaccctc ggtattactc aacaaacggc aaaatatggg gagctatttt
    13921 tcctttgtct tctctaatac tcaggtcaaa acgctgattt cacaacctct ttagcctact
    13981 attgaaaatt acaacttact tgctgcttct tcatcgatga tcacaacaac attgtcgtga
    14041 ttttgcaata cacttgctgg caaatgattc gttacaggtc cttcaatcat ccctttgatt
    14101 gcatcagctt tgctttcgcc aaatgccatc aagacgattt ctttactttt cataattgaa
    14161 ccgattccca ttgaaacagc ttttgttggt acatcttctg ctttatcgaa gaatcgtttg
    14221 tttgcattga ttgttgattc tgttaagtca actactgaag tcgttccgtc aaatgaagca
    14281 ccaggttcat tgaaaccgat atgtccattt tgcccgattc ctaagatttg gatatcgatt
    14341 ggatgtgcat cgatgatttg atcatacttt ttgcatgctg cttccagatc ttctgctttt
    14401 ccatctggta caaacgtttc tttgaatggt tttttatcaa ataattggac atccatgaaa
    14461 tgacggtagc tttgttcatc tgaaccgcct agtccaacgt actcatctaa gtttacagaa
    14521 gtcatttcac tgaaatccaa atcgcttgaa gtcatttctt tatataaagt aactggtgtg
    14581 cttcctgttg ccaatcctaa tacttttgca cctttgttca taccttcttt gatgatttca
    14641 aatgcttttt ttccgccttc ttcagcgttt tttacacgaa tgatttccat agtgattgct
    14701 cctttatatt ttggtatagc ccaattatag cattcttttt ttctttttac aataattagt
    14761 atagaccaaa ttgcattttt ttcattttct tgacaataaa taggaatccg tgttaggttt
    14821 cataagatgg ttcaatcaaa caatccatgt tgcatgaaaa ccaagcaagt gattcactgt
    14881 ggacgcttta tgtctgcagt ttttttatga aaaaggagtt attatgagta tactgacatt
    14941 aaacaacatt acccagcaat ttggcgacaa aatcctctat gaaggtgtcg atctgcaatt
    15001 aaatgctggc gaacatcttg ggctgattgg acaaaacgga gccggtaaaa gtacattgat
    15061 taaaatcatc actggtgaga tcttaccaga tgacggacag atccattggc aaaaaaatat
    15121 tcacaaaggt tacctcgatc aatatgtgga agtcgatgaa acattaacaa tcgaagaatt
    15181 tctaaaaact gcgtacgcaa aagaatacga aaaggaagaa aaaattgctt ttctttacca
    15241 aacatatagc gagaccttcg atgattcctt gctggaaaaa gctgggaaac tccaaacgga
    15301 attagaccaa ggagtgtttt atcaaatcga caccttagtt gctgagatga gcagcgggtt
    15361 ggggatcgat gtcttaggtt tagattcact gttgaaaaat ctaagtggtg gacaacgttc
    15421 aaaggtgatt ttagcaaaac tacttttaga aaacccagat gttctgatct tagatgaacc
    15481 aaccaatcac ttagatgatc agcatatcca atggctgacg cagtttcttc aggattttga
    15541 cggtgcgtat atcgtgattt cccatgacca agaatttctt gatcagatca ccacccatat
    15601 cgcagacatc gagtttggaa agattactaa gtatactggc catctaaaac aagcattgaa
    15661 acaaaaggaa caaaatcgcg aaagctattt gcgccaatat catgctcaac aaaaacaaat
    15721 cgaaaagacc gaagcttata ttcgaaaata caaagccggt tcccgatcaa cgatggcaaa
    15781 aagccgacaa aaacaattag ataaaatcga acggctgacg cctccagctt cagcaagtaa
    15841 agctgtcttt gattttccat tctcgcctat tgtcacgaca ttagcagtgg aaacaactga
    15901 tttagtcatc ggatatgatc gaccgctact tgcacccatc aatttgacca tccgctttgg
    15961 tgaaaatatt gccattcgag gcttcaacgg gattggaaaa tctactttat taaaaacact
    16021 gatcggtgag attgaaaaaa taagtggcga tttccacttc ccagataaca caaaaatcaa
    16081 ttacttttca caagatctgt actgggaaaa tccattagaa acaccactac aatatttaag
    16141 cagtcaattt ccaaaagcga ctatcaaaga attacgtcgc caactagcta aagcagggtt
    16201 agttaatcaa ttagcaagtg aacctctcac tacattaagt ggtggggaac aaactaaggt
    16261 gaaacttgca cagttgacaa tgaaccaagg taatttactg attctcgatg aaccgacgaa
    16321 ccatatcgat caagaaacga aagaaagctt acaagacggg atacagaaat ttccaggaac
    16381 agtaatcatc gtctctcatg aacaagaatt ttatcaagat cttgtgcaac gagtcattga
    16441 gattgaacca aaataaacgt ttgattgaat agttgaaaaa aaggagctgt gacaaaagtt
    16501 ttgtcacaac tccttcttct gaataaatgg tggccaatta gctgaccttc ggtattagct
    16561 caaaaaacgg caaaatatga ggagctattt ttatttggct attcttatta cttgatgcag
    16621 aacagctttt cacaacctct tttcgtctat tttttatttt cgagatttct ctttccttta
    16681 aaaacaactg tttgaaagaa gaacgcacat ccttgaaaaa caagcccgct aaccgttaca
    16741 cctaaccagc catactgctg ccacattgtc gtccctagca atgaaccagc cgcacctcca
    16801 ataaagtaca taaacataaa tacagacccg tggtgctagt taattttaat atcctaaaga
    16861 atatctcaaa gttccttcaa aacgtcgttg tgctcgactt aatagaacac tgtaagccag
    16921 taatatgctt tcaaatctac tctctagccc tttaacagaa cgtgagttga aattttgaca
    16981 acctaatttt tctaaggaag aaaaaacagt ctcaactgtt tttctttgtt ttccaagtaa
    17041 gctgtggtct agtttttctg atttatccat gtttttacgt ggcggaacgg atatagtaat
    17101 gcccttaagg gcaagctttt catggatttt atggctaata tatcctttgt cccctaggat
    17161 attgggaaga ttcgcttctt ccgataaagt ctctaacact ttcacgtcat ggacacctgg
    17221 attcgtgatt gagtaagtaa tcggaaagcc agtcttagta acgatcatat gaattttaag
    17281 accataaaaa taagattttt ttgttgagtt atagccaac
//
LOCUS       DACDEG010000048        17173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.050, whole genome shotgun sequence.
ACCESSION   DACDEG010000048 DACDEG010000000
VERSION     DACDEG010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.050"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(435..1004)
                     /locus_tag="IV142_001678"
     CDS             complement(435..1004)
                     /locus_tag="IV142_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8485339.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1078..2367)
                     /locus_tag="IV142_001679"
     CDS             complement(1078..2367)
                     /locus_tag="IV142_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485340.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2721..3569
                     /locus_tag="IV142_001680"
     CDS             2721..3569
                     /locus_tag="IV142_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP8485341.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPVPVR"
     gene            3583..4656
                     /locus_tag="IV142_001681"
     CDS             3583..4656
                     /locus_tag="IV142_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP8485342.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4646..6817
                     /locus_tag="IV142_001682"
     CDS             4646..6817
                     /locus_tag="IV142_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HAP8485343.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(6929..9085)
                     /gene="copB"
                     /locus_tag="IV142_001683"
     CDS             complement(6929..9085)
                     /gene="copB"
                     /locus_tag="IV142_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP8485344.1"
                     /translation="MHMGHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHMGHNHG
                     DIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGMEFPFQ
                     VTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYIYSVYS
                     FIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPESVKRL
                     TKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGIKKQAN
                     DKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAKWLFYV
                     ALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAAKNGLL
                     LKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGALEANAN
                     HPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLGLNVEQ
                     ALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVMLTGDNK
                     NAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLARASIGM
                     AIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILAIPLAA
                     GILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9228..11414)
                     /locus_tag="IV142_001684"
     CDS             complement(9228..11414)
                     /locus_tag="IV142_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP8485345.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11414..11623)
                     /gene="copZ"
                     /locus_tag="IV142_001685"
     CDS             complement(11414..11623)
                     /gene="copZ"
                     /locus_tag="IV142_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP8485346.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11636..12076)
                     /locus_tag="IV142_001686"
     CDS             complement(11636..12076)
                     /locus_tag="IV142_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP8485347.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12150..12623)
                     /locus_tag="IV142_001687"
     CDS             complement(12150..12623)
                     /locus_tag="IV142_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
                     /protein_id="HAP8485348.1"
                     /translation="MGRENRPSQGRSKKSPAFRIQAKKWFLQEQQSFMVSNPSWIVEG
                     NYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDMPESFSEKLNRK
                     YLEFLSFIWHFEGNNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN"
     gene            complement(12933..14399)
                     /locus_tag="IV142_001688"
     CDS             complement(12933..14399)
                     /locus_tag="IV142_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8485349.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(14710..16794)
                     /gene="cadA"
                     /locus_tag="IV142_001689"
     CDS             complement(14710..16794)
                     /gene="cadA"
                     /locus_tag="IV142_001689"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP8485350.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
     gene            complement(16936..17124)
                     /locus_tag="IV142_001690"
                     /pseudo
     CDS             complement(16936..17124)
                     /locus_tag="IV142_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
BASE COUNT         4899 a         3629 c         2976 g         5669 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat cgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa gtgaaaaaaa gatcaatgaa tcaactagaa aactatatag aagagcaaat
      241 aaaaaagcac tcaaattgag tgctttttta agctcactat cactaaaact atcactaatt
      301 ttatttataa gcgaacatgt ttctatcact gattttttaa atctagcgaa aaacccttat
      361 cttaaaggcg ttcgttcaat tctttggcaa ggtcttcaaa gcctggcttg cctagaagtg
      421 cgaacatggt tttcttatag ctcatgtaga aaaattttct tgatgatgat tttatccatc
      481 aattgagatt gactgttttg ctctcctatg atttcagcta tgattgtccg attttgcggt
      541 tttcttctta actggtagtt gattttccca gtttccatca actcgctaag catatccacg
      601 actccgccaa atccggtctt tttcgtaata aaacctagtg ctaccattcg ttcatctaga
      661 ttttttgctg taatcgtcac accagtaatg agcgagtgct gtaatgtttg ggccagctct
      721 tccatccctt cagcattgga tacgacggaa gctgttttgt taataggttc ttcgtttatt
      781 tcttttggtt ctttcctctc cactttcact gtttgacgaa ggacatctag ttcttgcaag
      841 agcgtttcgt attgttcttg ggtcagcatc acgccagcta ctttttcacg attaaatata
      901 tatacgccag ttcccgcgtc tcgcgcttga gcaaatacat ccatcggcga tcgtttcact
      961 tctgtgatcg aagatgtggg aacttctaat ttctttaatt ccaatggatt tcctccttta
     1021 ctaatcgctt tttattatag cacagactga cttgttttga tcttttttcc aaaggtttta
     1081 tcggtttttg cctaaccagt aattgatatc ggtaagaagt ctttcgatac caaatgaagg
     1141 atggtacaga tacgtttttt gatcttcgtt atggtctagt ggaaaaaaat gatcggtgat
     1201 cactagatct gcataccgag gcggcttatt tcgagtacat acatgtatag agatccgttt
     1261 tcgatgtctt agcgttttgt tcagtacccc agcaagtctt cttgcttctt ggtgttcata
     1321 gcgaaaaaaa aagacgattt tcacttcagg agggtggaca tctaaatacc ctcgttcctg
     1381 gagtgttttc agtaaaagca tcccccactc tactgcgggg tgtgcaaatg cttgttggat
     1441 cgctagccga aattgatata gtttatcttt ggaattggga aatttttcta atgtttcctg
     1501 ataataacga acttcttcta tcagccggtc atggatacca attacgtaac gaagccattc
     1561 ttggtaaagt acttttgtca gctttgattt gttttcgctc tcaaattcgc tattcagcca
     1621 aaacagcgaa tcaagctttt cagtcacaat cgtcaatttc cgatagtctt gtgtctcttc
     1681 tatccgtttc caccgctttt cagtaatctg gttacagctc aagcgcaaaa ttgctagata
     1741 atttttttct tttattggca aaatctgctc ttctctaaat agcaattcag gaaacaattc
     1801 aaagaatagt gttcgactgt ctcctaaaat ctcgatttct gccggatcct ttctttgctt
     1861 caataattgg atgaacatct cagaaaagga ctgctctcca tcatctgcaa aaaaatgcgg
     1921 aaaatctttc aaaaagcaaa acagcagata tcttactttt ctttctgaac caaagatctg
     1981 tgctttttta taagaaaaag aaaggtggta ctcgccaaac cactttctca atcgctcttg
     2041 cgtagaacga tcgtacttct gctcgtaagg ttcattcaat agcagatttt tcagtaaaca
     2101 aaacgggggt gattggatca gaaaaacaaa caagagataa ggttcgctat atccttgatc
     2161 ataaactaac tgaacggttt tcttatgaag aatcagctgg atgggacagt cgctttccgc
     2221 caaacgtttc agatgccgat ccactgtttt aacatcgatt ttcagttctt cagctaaatc
     2281 agcttttcgc cagatcaaat ctttcattag attgattagt gaaaataaat tctgtgcatt
     2341 ttttgataat agtttttgcc atctcatcga tcatttctca cctgatttct aaacatattg
     2401 tttgatcaaa atgggttcac tcctaaaaag ataaaaaact ccctttctat ttatttaaat
     2461 acgcaaattt tcctcattat ttttaatttt actcaattta atcgtattct catttcaaaa
     2521 cgaatgtact ttaaaaaaac ataataaaat cactttgctt gtaggatgta ttcccttcca
     2581 ttacaatgaa ataaatagca attttatgag aaggtgatgt gaaaatggaa gaaaaaatcg
     2641 aagaatccaa acaaaaaatc gatcagttga tacaagaatt agaaaaagcg gtcatcggca
     2701 aacgttcaac tatccaattg atgatcgtgg ccctattagc aggtggtcat gtcttattcg
     2761 aagatattcc cggtgtaggt aaaacgttac tggtcaaagc actagcaaaa gctgtacagg
     2821 gaaccttcag cagagtccaa tgtactcctg atctgctgcc tagtgatatt cttggttttt
     2881 ccgtatacaa ttcacaatca aaagagtttg aatttcgtcc gggaccgatc tttaccacga
     2941 tcttgttggc agatgagatc aatcgcacga ctcctcgtac tcagtcagca cttttagaag
     3001 caatggccga gaatcatgca acgatcgaca acaatacata ccctctagac aatcattttt
     3061 ttgtcttagc tacgcaaaat ccaatcgagt atgaaggaac atatcctttg ccggaagcac
     3121 agctcgatcg ttttttattc cgactacaaa tcggttatcc ttcttttgat gacgaacttt
     3181 tgctactttt agataaattc gaaaaagtgt tggataactt aaatgaagta ttgaacagtt
     3241 atgaaataga agaaataaaa acaaatgtgg cgaatgtctt tgtcagtccc gaagtggcat
     3301 cttatgcctt gcagttagtg tcagcaacaa gaagccatgc tgccatccag cttggcatca
     3361 gtccacgagg aagcctttcc tttattcagg cagcaaaagc ttatgcttta atccatggga
     3421 gaaattacgt cacgcccaaa gatttgcagg atttagttcc ttacgtgttt tcccatcgcc
     3481 tgatttttta cgatcgttca ttgaaggaag acgaaaaaaa acaaatcatt catactcttg
     3541 ttgcacaaat tcctgttcct gtgaggtaga ttgaggtaga taatggaaaa aatcaaagat
     3601 tatttcatta attttgtgtt atttctggta tatttcatca ttgtgctttt tgctcttgta
     3661 ttcaacaatt ctttaggctg gttcttgttg gcctttttta ctctttatgt atcatggagc
     3721 ttgatttcat taagcagctt tctaaagaaa ctttcggtta cttgtacatt gcagccactc
     3781 gtttatcgca aaaaaagtat gcaggttatt ttttccatag caaaaaagag aactttttct
     3841 ttacctctcc ctcgtttaca aatcggcttc ccagcaatct ttgcagaaaa aaaacaagag
     3901 attttgattc ttacaaataa accgcaagaa gtccaattgc tttggcagcc aaaagaaaga
     3961 ggatgccatg aaacattaac tgtcattttt acagcctatg atagtctagg acttttcaga
     4021 aaaagtgcaa aaagagagat ctcttttcct gtgactgttc tgcctgcttt ttgtaagatt
     4081 caagcagaaa aattacagag agttctggaa aaaaaacagc agctgaacgc gtaccaaaga
     4141 ggtcttgatt ttcgggaaca tcgcgcttat cgtccaggtg attctttcaa ccgaatcgat
     4201 tggaaattgt ctgctcatac tcaagagtgg ttataccgtg aatacgatta tcaagaagaa
     4261 gaatgttctc ctcttttttg cttttggggt tcgcacagtc caaaatttga acctctgcta
     4321 gacttgtatt tttctttatg gcatttgaga aaagaaaagc agccggaact tctgatatta
     4381 ggcaaccgcg cctttcatgg caaagacccc ggtcatacct attttgcttc tttggaagct
     4441 ggcgatgaga aagctttttt cgcacaccta aaaaaatatg caaaaaaaca aatcgtcctc
     4501 tttgttcctg agaattctcc tgaagtctca gaagctattg aaacattgtc aaaagatcga
     4561 acggtttatc ttgtttattt cgtagaaaaa caactcatgg tccaagaaaa agacaagata
     4621 tgttcattgc caggaggtga atggattgac gattaaatgg gaaaaatggc tgtgtggcgt
     4681 ttactccttt gtgatactaa gtgtaggtgc tccgatattt gtagtagcta atcaattgca
     4741 aaacagctac cttcttatcc taactattgg tgtcctatgc cttattgtct gctgtattcc
     4801 taaaaaatgg gtacgtttgc tgctttcttt gccagtaatg ataggttgtt tgtttttgta
     4861 ttttccttcg aagacatttt ccttattatg gatcttacag tttttttcgt cagtcacaga
     4921 ggaattcttc catctatccc aaggtaaact ttattatttg ccagaaaaga cagctttttt
     4981 tctcataatg ttgctgatct atttatttat tacattgacc gtggattacg actatttgta
     5041 cgcacccttt ctttctttga tggcttactt catgatgctt accgtttttc gggaaaaaga
     5101 tttactgtac gaaatgattg cggtagtaac tgttttgttc gtgtatctgg cagcaaggca
     5161 atttttctcc atgcggttgt tgtcgggaaa cactcgattc cagtcattga ctacagttct
     5221 gcttatttgt ttcttaacta ttagcgctat cttgccttta tatcttccga aagtacacca
     5281 atctctcgag gaaacgagtg aaccgatccg agaatatctt aatgatgaag ggctttatga
     5341 tacactccat gagtatcaat taaccggaag tgcaagaaca ggcttcagtg agaatgatga
     5401 acagcttggt ggacctttat atgataatgg cgaagttgtt tttacagcga accaaaaagg
     5461 aaatggctat tggaagatcg aaaataaaaa tcattacacc ggttctggat ggatagaaga
     5521 tcaggacgca cccatccaac caatccaaga attccctgtt gacatcacga gctaccaaac
     5581 cgttatggaa gacaaagaaa acattacatt gaccctaaaa aaacaagctt tcttacctta
     5641 tccttatgga aatactacct tgactgattt ttcattagct agtggacaaa cgctttacta
     5701 tttgcctgaa agccagcgat tcattcctgt cccgaatgaa tcgacacgtt ctgcaacttt
     5761 acaaacagaa aggttagttt ttacgagaga agaattaaaa agcagtcagt taaaagctga
     5821 tgatttagaa aatcagcaga caaatcttca attgccaaaa agtttgccta aacgtattga
     5881 ggacttggcg gtcgaattga ccaaaaatga agcaacttta attcgtaaag tcgaagctgt
     5941 cgaacagtat ttaaaaacaa gcggaaattt ccgctattcc aaaacggata ccggacatac
     6001 tccgaaagaa cacgattatg tggattattt cctctttgat tcaaaagtcg gctattgtga
     6061 caacttttca acttctatgg tcgtccttct tcgaacactt ggtatcccag ctcgctggac
     6121 aaaaggtttt acaaatgggc tcagaacagg cacagataat caagggaatg aggaatacag
     6181 cattctcaat agtcatgctc attcttggcc agaggtatac tttcctggat ttggctggct
     6241 tccatttgaa ccaacccctt catttgccaa tccaatgggt tcacaaccag ttgctgaaac
     6301 aaccgaaacc gtccagcctg ctatcgaaca accaaatgaa agcgaaaata tggcagaaac
     6361 tatttccagt gagagatctg aggtcattcc atcagaagaa acaacactca caccagctta
     6421 taaaatcgtc ttattcagcc ttggggtgtg tttatacgtc ttgttgttct ttctattgtt
     6481 attcagaaaa tctctgcgct ggacaagtta tcttatctat cttcattttt ttgcttctac
     6541 taatacgatt gcatatcaaa aacttcttaa ccatttggaa acattggtac cgcgcaaacc
     6601 cgcagaatct ttgcaggaat atagcctgcg aatagaacaa gaattcaagc tagatctgcg
     6661 tttcattcga ttaactactc aatatgaagc agaaatatat ggacgacaag cagatccggt
     6721 aaatttcgat aaaaaaacct atttagaaat tacgcatata ataagaaaaa cggtatacaa
     6781 aaaagttctg acaaaaggga gccgcttcaa aaaataagat aaaatttctg gagaatctac
     6841 ttctggaatg ccgagaacca gctgcttgag tatcgattat tcgaaaaaaa ggttgtgaca
     6901 cgttttttgt cacaaccttt tttcattatt atttgatttt caatgtcaaa gcatttagag
     6961 ctacaactac cgtacttaac gacatcaaga cagcaccaac tgctggactt aatatgatac
     7021 caattggcgc aagaattccg gctgctagcg gaattgcaag aatattgtaa cctgcccccc
     7081 accagaggtt ctggatcatt ttctttcgcg tttgcttggc tagatcaaga aaatgcaaaa
     7141 tatcttttgg atcgctgttt gtcaatacga catccgcaga atcgattgcg atatctgtcc
     7201 ctgcaccgat tgccatcccg attgaagcac gggctaaact tggcgcatca ttgatcccat
     7261 cacccaccat gattactttt ttcccttggt caaggtattt ctggacaaca gcttctttat
     7321 catctggtaa taaaccgcca taatattctt cgatccctaa ataatctgct actgcttggg
     7381 cagcgttttt attgtcacca gtaagcatga caggagtgat ttttctttct ttcaatgttc
     7441 ggataaattc ttttgcttct gtttttacta cgtcacctaa agcaacgatt gcagctagat
     7501 gattttctaa aatcaggaag ctgatggtat ttccttgttc ttggtaaggt ttcaataatg
     7561 cttgttctac gtttaatccg agacgctcaa cttctttttc attaactatt ttcacttgtt
     7621 ggtttttgac tgttgcctct agcccaactc ctgaaagatt ttttagattt tcggcttgat
     7681 aaggcttgat ttcatgttct ttcaaataat tcataatacc aacagccaat ggatgattgg
     7741 cattcgcttc caatgctcct aaatattgaa ggatttcatt tttattgaac gcatcatcaa
     7801 gtacttcgac acccgttaca gtaaattttc cttctgttag tgttcctgtt ttgtccagca
     7861 tgatcacatc tagatcattg gcttgctcca gtgcattgcg gtttttcaaa agtaatccgt
     7921 ttttagctgc tattgatgta gagcgagcaa caactaatgg gatagctaaa cctagagcat
     7981 gaggacaggc gatgataaag actgttacca tacgttctaa tgcattagga agatctgtca
     8041 agaacaacca ggcgatgaat gccaggatac ccacaattaa tgcgacataa aacagccatt
     8101 ttgccacttt gtcagataat gactctagct tggatttttc tccttgtgct tttcgcacca
     8161 tttccataac cttcgctaga taaccatttt caccagttcc agttacttcg atttcgatcg
     8221 ttccatcccc attgatagaa ccgccaatca ctttgtcatt ggcttgtttt ttgattcctc
     8281 ttgattcgcc agtcacagct gattcgtcaa caattgtttc acctttcaag attacaccat
     8341 ctgttggcat tttatcacca gaccggacga tcaaacgatc accttcgttt acttctttca
     8401 atgaaactgt ctcttctttt ccatctttcg tcaagcgttt tactgattct ggcagtaatt
     8461 ctgctagttt ttgtaaagca ttgctggcat tcgacacagc attcatttca atccagtgac
     8521 ctagtagcat aataacgatc aaagtagcca attcccagaa gaaatccatg acgtgtgtat
     8581 gtggattgag cagatttgcg ataaacgaat agacactata aatatatgca actgtaatcc
     8641 ccatcgcgat cagtgtcatc atagcaggac ttttttgttt caattccatc ttagctccac
     8701 ttaaaaatgg ctgaccgcca tagataaata gtatagttgc taagatcaat acgagccaat
     8761 cagaaccagg aaacgttact tgaaatggaa attccattcc catcattggt gaaaaaagaa
     8821 tgattgggat agctaggata agcgatagcc aaaatttttg tttgaaattt cccatgtgca
     8881 tggaatgatc catgccgcca tgatcgtgat ccatgtgcat cgaatggtcc atttgtttgc
     8941 tgtttgctat atctccatgg ttatgtccca tgtgcatgga atggtccatc tggctatggt
     9001 catgtttcat ctccatctgt ttttcatgag attctgtact ttctttgttt actacttctt
     9061 ttttatgttc atgtcccatg tgcatggaat ggtccatctg gctatggtca tgtttcatct
     9121 ccatctgttt ttcatgagat tctgtacttt ctttgtttac tacttctttt ttatgttcat
     9181 gttttttctc catatcgttc ccattcattt ttactccatc ccttctctta ctttttcatg
     9241 tgccgattta agctcaggga gttcaacaat acactgacgg aactaaacgc catcgctcct
     9301 cctgcaatga ttggattcaa aaagcctagt gcggcaaaag gaataccaat cgtattatag
     9361 acaaaagccc agaaaagatt ttgtttgatt tttctcaatg tcaccctaga caactcgatt
     9421 gtttgtgcaa tggatgccaa actgctactc atcagcgtca catcggctgt ttccatcgcg
     9481 atatccgttc cacttcccat tgccatacca acatcagcta atgctaaagc cggtgcatca
     9541 ttgattccat cgcctgccat accgactttt ttgccatctt tctgaagttt ttctacgtag
     9601 gctgctttat cctctggaag tacttcagca aagatatgct ttggatcaat accaacttgt
     9661 ttaccaatcg tttcggctgc cagtttgttg tctcctgtca gcataaaaac atctaatcct
     9721 ttattctgga gctgcttgat tgcttgtttg gcttctagtt tgatttgatc agccacggca
     9781 atcaacccga tcacttgctt ttcatcagct aaaaacatga ctgtttttcc ctgctgttct
     9841 aattctaacg cgtgttcttg gtattcatca aatgataggt tcaattcaat taatcgttta
     9901 cgtgtacccg caaaatagcg tactccgtta atcgtaccgc tgattccagc acctgggtga
     9961 gctgtaaaat caatcatatc gtatgttgct gcttcttgca gttttccgta ttccacgatt
    10021 gctttaccta gtggatgttc agaagcttgt tctaaagtat aaaaaataga taggacttct
    10081 ttagaaccga ctagatctgt aactttgggc tttccttctg taatcgtccc agttttatcc
    10141 aaaacaatgc tgtctaagtg agcagctgct tctaatgctt ctccgccttt aattaaaatt
    10201 ccatttcgtg caccaacacc cgttcctacc ataatagctg ttggagtcgc taaacctaat
    10261 gcacatggac acgcaatgac taatacagag acactatgga tcaatgcgag ttgccaatca
    10321 ccagtgatca aacctgtagc tatgagcgtg ataaacgcga tgactaatac gatcggaaca
    10381 aaaatccctg agatccgatc tgcgattttt tggattgggg ctttgctccc ttgagcatct
    10441 tccaccattt gaatgatttg cgctaatact gtctgcttgc cgacttgtgt gacttccatg
    10501 tgtagcagac cattcgtatt gattgtcccg ccaaaaagcg tgtcatccgg attcttttct
    10561 acaggaagat tttcaccagt aagcatactt tcatctatcg cactgcttcc tgaaataatt
    10621 cgaccatctg ctggaatctg ctcaccagga cggatgacta gttgatcacc aacgactact
    10681 tcttcaatcg ctaacgtttc ttctttcccg tttcttatca cttgggcggt ttttgtttga
    10741 agagacatca attgtttgat tgcattacct gttttcgttt ttgctgtatg ttccaaatat
    10801 ttgccgagca aaatcagcgt gatgatcata ctgctgcttt caaaataaag atcttggggg
    10861 tgtccacgga aaaatccgtt gtaaatgctc aatgcaaagg cagccgatgt tccaattgct
    10921 accaatacat ccatatttgg tgctttcgac ttgattgcat ggtacgcacc tttataaaaa
    10981 cgagcaccga cataaaattg gacaggagca gacagcacta actggacgat tggaaaatga
    11041 aaaaagtgaa caatcgccgc atggcttcct aacatcatag caatcattga caacatcaac
    11101 ggcaaagtca aaaccgtact caatataaga tcgaatagca tttttttcaa atacgcttgc
    11161 ttttcttctg caattttttg tttgtgggct tcatcgtaca aaattgcacc atagccgatg
    11221 ttttctacgc tttggattaa tttttcagtg gtcgtccctt catatttgac acttgctttt
    11281 tcagtggcca agttgactgt ggcattgacc acaccaggtt gtccgttcag ctctttttcg
    11341 atcctagcag aacaatttgc acaagtcata cctgtaatga cgaacgtatc cattttggtt
    11401 tctgctgttg ccatcaaatc acctctgctt tataacccaa tccattgatt gcttgacaaa
    11461 tcttttcgga ttgaactttt gtttcatcaa acttgaccgt accgtttgct tttttcaaat
    11521 ttactttcac tttttgcacg ccgtcaagtg cgcttactgt ttcttctact cttgctgcac
    11581 aatgattgca gctcatacct tcgattgaaa attgttgttt cattttttct tcctcctatt
    11641 gatgttgttt acattcacat tgacctggga tacagttaca ttcgatcgta tctactggtt
    11701 gcttttgttc cagttgtttc aaaatctcat tgatatcttc ttgagtcaaa gtggcttctt
    11761 ctactagatc cgcaatcgtc tctccaatcc tttttgcaca gatatgggaa aaaagatttt
    11821 ccgtcgcact gcgaactgtt tccatctccg agacagctgg atgataaatg aatttttttc
    11881 cttcttgttc tgtccagatc acttcttttt ttaccaaacg acccagcaaa gttttgatcg
    11941 ttgctggttt ccaatccatc gaatcagcta agatctgcgt gatttcttgg gctgtcgtct
    12001 gtccaagtgt ccaaataaca cgcatgatct cccactctga atcactaatc tttactggcg
    12061 ttttgacttt ttccatttat ttcacacctt tcgtttacaa atgtaatcat taacttatga
    12121 atacagatta cacttgtaaa caaaaactgt caattatttt tgctctctat tttcaattta
    12181 gttaaaaatt cttgtttctc tcgtcttgtt ttaaggatga tcagtttatt tcttgcatta
    12241 gctacttgga tcagctgctg tatttttggt tcattgtttc cttcaaaatg ccagataaaa
    12301 gataaaaatt ctaagtattt cctgtttagt ttctccgaaa atgattcagg catatctggc
    12361 cgagattttt tgtttatctt tctttttaga gaacgccaaa ctactcggta gatggcgaga
    12421 taacgcggca ctttaagcca aatgatctta tctgcttctt taatcctttc ctctaacgta
    12481 gaagtataat ttccttcaac gatccatgac ggattagata ccataaacga ttgctgttct
    12541 tgcaaaaacc acttttttgc ctgaatgcgg aaagccggtg atttctttga tcttccttgc
    12601 gagggacgat tttcccgacc cactttgtcc aatcacgatg atcttcattc ttctccctca
    12661 cttctctttg gaatttggtt cgatcatacc acttataacg ccgcaaaaaa acaagggact
    12721 gtgacaaaag tcgtgtcaaa gtcccttgtt tcgaataaat ggtgttcaat cagctgacct
    12781 tcagcattag ctcaaaaaac ggcaaaatat gaggagctat ttttgtttgg ctgttcttat
    12841 tacttgatgc aggacagctt tttcacaacc tctgattcac ggtgttccag aagtagcttc
    12901 tgtcacaacc tcttgtaaca agtatttcta ttttaaggct gaaccactgt ttctggttca
    12961 gtaatttctt tgtttttgtc tcgacgagca attgctggca agatcatacc taataagatc
    13021 aagacaattg gtgtgatgat attcgtgaat aattggaacc accatgcttt aggatcagca
    13081 gtataatcta cttttggcac cattcctagg atacatgcaa acgcagtgaa ggcaaagcac
    13141 caagcaccaa agataaaacc gatttttgga tttttaacaa atttatattc agaattgaat
    13201 ttcttataag ctttattcaa catcatatat gccaagaaca cccataggta acgcattggc
    13261 atgacaactg agttcaagtt cgtcaaccat tttactaatt cattcatatc tttgatacca
    13321 aagatcggca acaaaataat gattcctact agtatacctg tcagaagata accattcacc
    13381 aatgtacctt tacgattacg tttacgtagc cagtttggta caaattctgg atccgcatct
    13441 gctaacaaga tttttagtgg tgcatcaatt gaaaaggcta atgcggagat ttgtccaatc
    13501 atgtttgtca atgcgtaaat gatcatcaat gagttaccga taccatagta attacctaaa
    13561 atttgaaaag cactgtatgc cccatttgcc atcaagtctt tagggatatt atcgctagca
    13621 aacagcatac ccattgcgac tgagcctaat acagcacaaa tccctaccat tgctgctaag
    13681 aagatcatcc ctttaggaaa ttcctttgca ggatttcttg tagagttgac ataaggggat
    13741 attttttccg cccctccaac tgcaaatact agcatcgaaa cggttgtgaa ataactgaaa
    13801 tcaaatgttg gaatatatgt tttcacttga ttcatatgtg gtgtggcaaa ttccatcgtt
    13861 ggattgatga acggtgccgt tacagctaaa agaataaata agatcgacat gacgaacatc
    13921 gctgttccgg ccagtccgcc aatcactttc aaagtagaaa gtccttttgt tgataaatat
    13981 aaaaaggcaa gaaatataat aagtgaaata actgctacca tttgtactga catcgtattt
    14041 acgatatttc catttccttg gactgcccaa ccaccagcaa tcagaattgc ttgaggcttt
    14101 tgtgccaaat aaggaatatg gacgacccag taagtccaag cggcatagta tgccagtcgt
    14161 ttattagatg tcgtattttc gacccatgaa ctgacgcctc ctttactatc cttgaaggct
    14221 gatcctaatt gtccaacgat caatgcgtat ggaataaaat aaatggccaa aatcaatagc
    14281 caagaggtga caactgatac tccttgctga gcatagttat taactacatt ccccatgccc
    14341 caaaccatgt taaatgcaat taacgcaaca gtaaaccagc gtaattgttt gttgttcatt
    14401 cgttctctcc tttatgataa aaatttgtta tccagatgta atcatattca atttttaaca
    14461 aacttacaag acagtgttat tttatgtgtg tattctttta tttaatacac atcgtttttt
    14521 catatttttt ccatcaaaaa aataagttat tttcttttat ttcattgacc ttattgaatt
    14581 tcaagctatt ttgaattttc tgcaaaatta aaaacattag ttattttata atcctatcta
    14641 ttttctttac atttttcatt aagaaaagga tataaaaaaa agaggacgtt cattcgtcct
    14701 cttcattgat caacgtttat tttttacatg caagcttcgc atggcattca aaacagctaa
    14761 tactgtcacc ccaacatctg caaatactgc atctcccatt gatgcaaaac caaaagcacc
    14821 taaggaaagt accaaaattt ttatcccaat agcaaagaca atgttctgtt tgacgatttt
    14881 caatgttttc tttgctaaat ggattgcttc tgcaatcttc gatggttgat cattcatgat
    14941 gactacatct gctgcttcaa ttgcagcatc gcttcccaat ccacccattg cgattcctac
    15001 atctgctctt gctagcaccg gtgcatcgtt gattccatct ccgacaaatg cggtcttttt
    15061 attgttactt tgtaagattt cctctaaccg ctgcacttta tcttctggaa gcaattcgct
    15121 atatatcttg tccacgccta cttgcttccc gatatgatca gcaatttttt tcgagtcgcc
    15181 cgtcaacatg actgtgtttt tcacaccagc ctgttttaat tgaaccaatg cgtctacggc
    15241 atcttctttc aaggtatctg caataacaag ataaccactg taactttgat cgatcgctag
    15301 ataaagcaat gtaccgattt cttgacttga aggaaaagag atttcaaatc tttccatcaa
    15361 tttgtgatta cctactaata cttgtttgcc atcaacttca gctaagatcc cttcacctgc
    15421 aaattcctgg atattagctg ttggtaaaag cggacctgcg tagccttcta aaacagattg
    15481 agcaatcgga tgtgtagaaa actgttctgc acttgcaaca tattgcataa atttctttgg
    15541 atcaattcct gttggatcga tcgtctggat cgaaaagtca cctttcgtta atgtgccagt
    15601 tttatcgaat accactgtct cagtttgtgc aagcaactcc aagtaatttg aaccttttac
    15661 taatacaccg attttacttg ctcctccgat accgccaaag aaactaagtg gcacggaaat
    15721 cactaatgca catggacatg agatcactaa aaatgtcaat gctcgataga tccattcaga
    15781 aaacgccgct ctagaaacaa caagtggagg taaaacagct aataaaacgg ccaatcctac
    15841 aacgactggt gtgtaatagc gcgcgaattt agtaatgaaa ttttcagctg gtgctttttt
    15901 actgctggca ttttctacta attctaataa tttgctgata gtagaatttt cataagattt
    15961 ttctacttga actaaaaatg gtttattttt attgataaat ccgcttaata ctgtttcacc
    16021 aacatataca gatttaggga ctgattctcc tgtaagtgct gacgtatcga ctaatgattc
    16081 tccttcgatg atcgttccat ctaacggtac acgttcacca ggcttcacaa gtacaaactg
    16141 accgatcagc acgtcttcag ggttcacggt catcaatcct tccgccgtct gcacgtgagc
    16201 tgtttctgga cggatcgcca tcaattcgcg aatcgatttt cttgaatgac tgactgccaa
    16261 tccttggaaa tactcgccga tttgataaaa tagcatcact gctacggctt ctggatattc
    16321 accgatcaac atagcgccaa tcgtagcaac agacataaga aaattctcat caaagatttt
    16381 tcctttgacg atattcatca ctgccttctt cactacatca tatcctatca gcagataaat
    16441 caataaatac cctatcaagc gaattggttc attaaaatca aataatagca ataaagcgat
    16501 acctgcaagg gaaaataaaa tccggaacag cgtatttctc tttttttcat ttccatgttc
    16561 agatactttt tccttatcta tttctgttac tttgacatct ggttctaatt ccttcactac
    16621 tttttttgtt tcttgttcaa tcgtagccaa atgatcttcc tctgcgtcaa tcgttagttt
    16681 tccagaagtg aagtttaccg ttgcttcctt gactccattg attttctgaa ctcctttttc
    16741 gattttcatt gcacagttcg cacaatctaa accttctaat ctgtaacttt tcattttaat
    16801 gacctcctcg cctcaacata tgaatatctc ttcataacta attataaatg aatggttatt
    16861 catatgtcaa ggtatccgaa agaaaaaatg aaaaacatct gcatcggaaa atttcttcca
    16921 tacagatgtt ttggcttatt ctttcttttt ctcttcgatc attgccactg ctcttatcca
    16981 acccgcactt gccttcttga tcttgacagg atagcaacta agtttgaagc ctgttgctgg
    17041 caatttgtct aaattcatta atttttcgat aggacaatat cttttttcaa tccctgcaaa
    17101 atgtccttcc cagatatacg aagctccact acgctttcaa aaatatagaa agagttcttt
    17161 aatgaaaatt att
//
LOCUS       DACDEG010000049        16986 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.051, whole genome shotgun sequence.
ACCESSION   DACDEG010000049 DACDEG010000000
VERSION     DACDEG010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.051"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..639)
                     /locus_tag="IV142_001691"
     CDS             complement(<1..639)
                     /locus_tag="IV142_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8485351.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIIN"
     gene            complement(887..1723)
                     /locus_tag="IV142_001692"
     CDS             complement(887..1723)
                     /locus_tag="IV142_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP8485352.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1734..2663)
                     /locus_tag="IV142_001693"
     CDS             complement(1734..2663)
                     /locus_tag="IV142_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP8485353.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2855..3208
                     /locus_tag="IV142_001694"
     CDS             2855..3208
                     /locus_tag="IV142_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP8485354.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(3282..3938)
                     /locus_tag="IV142_001695"
     CDS             complement(3282..3938)
                     /locus_tag="IV142_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP8485355.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            4164..6098
                     /locus_tag="IV142_001696"
     CDS             4164..6098
                     /locus_tag="IV142_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8485356.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(6184..7407)
                     /locus_tag="IV142_001697"
     CDS             complement(6184..7407)
                     /locus_tag="IV142_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP8485357.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(7401..9299)
                     /locus_tag="IV142_001698"
     CDS             complement(7401..9299)
                     /locus_tag="IV142_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP8485358.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(9293..10411)
                     /locus_tag="IV142_001699"
     CDS             complement(9293..10411)
                     /locus_tag="IV142_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP8485359.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(10494..11327)
                     /locus_tag="IV142_001700"
     CDS             complement(10494..11327)
                     /locus_tag="IV142_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8485360.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(11320..12237)
                     /locus_tag="IV142_001701"
     CDS             complement(11320..12237)
                     /locus_tag="IV142_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8485361.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(12464..13588)
                     /locus_tag="IV142_001702"
     CDS             complement(12464..13588)
                     /locus_tag="IV142_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8485362.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            13771..14679
                     /locus_tag="IV142_001703"
     CDS             13771..14679
                     /locus_tag="IV142_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8485363.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            14820..16433
                     /locus_tag="IV142_001704"
     CDS             14820..16433
                     /locus_tag="IV142_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP8485364.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         5364 a         3305 c         2806 g         5511 t
ORIGIN      
        1 attaatgata taagctttta aatctttaca taaatactca tattcgagtg attttttata
       61 ttttttaatt gtgccgttta cgagaatctc ttcatctttc aatactaggc ctattttatt
      121 tttataacct ggcatgctca ttagataaag gatttcatcg taatggatat agtgatcgct
      181 attaccataa cggacagttg tccacctttt tttcgtagca tgttgaacag gatagttttc
      241 tttttctttc aataagtaac tttggatgct ttttttattt ttttcataag tgaattcttt
      301 tatcaaataa ccactaggcg taatctttcg attaataaca gttattgctt tattcggatg
      361 agacgttaga taaacaattt tacacttttc attcttttct ctaagcattt cgcctagttc
      421 gataccgcta taataggcgt tcaaatcgat gtctaggaaa aaaatatctg aggaatagat
      481 ttcattattt ttaatgaatt caacaaattg tataacgttc aatccctgaa cgatctcaaa
      541 tttccaagga cttaataaaa ttctggattc ttccaaaatt tctactagca ttttttcaaa
      601 aaccaaacta tcatccaaaa taaatatacg cgttaccata aaaagtacct ccactcgata
      661 ttttactatc aaaatcattt catacaactt ttcaacatac atgttaggaa taaattgtaa
      721 ttctcttcac aatcttttcc aacacctttg aaaccgatat caaatacttt caggatgctg
      781 cttctaaaat aaatatcaaa caaatgaata catcatgcaa aaaaagccag acaaccgacg
      841 tccttgcgtg tcagctgtct gagctatgtt tatttatttt gttcttttaa aaagaacgat
      901 acacatactg atttttcgga gcagagactt tctcagcgtt tgtaacaact acagatggga
      961 tttcacgctt gaatggttga taatagtctg attggatggt tccttctatt ttataccatt
     1021 catcattttt aaccgttact ccttccggca tatgcgtcaa caaaccaaag actcctgaat
     1081 ctgccacaca atggataatg ccgaatcgga acaagaagat atctgtattg ctatcgttcg
     1141 gtgtttggta aacaaatcct tcatagctga tttttttgcc aatgaattcg ctagggtaat
     1201 tataaattag ctccataact tctaaatagt tttcatctgt gatcttgata gtttgcttat
     1261 ccccatactt ttctttcaac tcattcattt ggtctaagta gtctgatttg ttgaaataaa
     1321 tactggtatc cggctttaag tattgtgtct gcatctccgg atcccctact gattctttac
     1381 ttactgggaa attaaatcct ttggcttcta cgatcgtagt gtcgagacta actgttggaa
     1441 acaacgtccc cacgattact ggcaaagcta gcagtacata agcgattcca cgttgataag
     1501 gttttgacaa cccatggtca tgatcgtgct cgtgtgcctt ttcttctgct tttccgtttt
     1561 tgacccacaa atacagctga actaaagcca aaagagcaga taaaatcatt gaaagaatag
     1621 ctaagtagcg ataatggaca ttgatatatt gatcgagttt gccagaaact tgcaagtaca
     1681 tcatcaattc gaaataccca gataaaatca aaaaccgcag catattttcc ctcctaaata
     1741 aataatgtga atccaataac gctaagggca atcagtccga tcagcgataa aataaagcgt
     1801 cctgtaaaat atcgtttcat catcagtaga tttttgatat cgaccattgg gccaaagacc
     1861 aaaaaggcag ctacaggtgc tgtgccgaac agacttaaca aagaagatcc tataaacgca
     1921 tctgcttctg agcaaagaga catcgtaaag gccaataaca gcatgatgat aatggctaaa
     1981 atctttgttg agccgagcgt tagcataatt ctagttggta aataagtctg catcgcagct
     2041 gcgattaatc cgccaatgat caaataacga cctgtatcaa aaaattcgtc gattgaatgt
     2101 gtcaacacat gcccagtttg atggatcagc tgattttttg gttcattctt ctcactttgt
     2161 tcttcttcat ggacatgatg atggtgattt tctgctactt tagacgtgat tttttctttc
     2221 aatacagcgt ctttttgaaa ataggcaagc cagacaccaa tcacaagggc aacaaaaaag
     2281 ctgcctatca cccgccagct tgccatctgc caagaattgc caaatgcgat aaaagtggaa
     2341 aaaatcacga ttggattgat gatcggagca gtcagcataa aagcaaacgc tgtgtgaact
     2401 ggtacgcctt ttttaacaaa ttgatgaacg atcggtacga tgccgcattc acatgaagga
     2461 aagaaaaatc ctaagatcga accagtaaaa atagagaaga tacgattttc aggaagccat
     2521 ctcttcacac gttctggggt caaaaatact tgtaaggcgc ctgagataag acagcctaat
     2581 aatacgaaag gtaatgcttc aataacgatc gataagaaaa tcgtactcat ctgcaaaacc
     2641 gaatttggaa taaaagaaaa catgagtttc ctccttttta tttctagttc attcgctttg
     2701 caaatatacg cttttattca aaaaaaacaa gttgctctgt aaatttttac acaactctta
     2761 ttttttacca aagcacttca attgactttt tctgttattc ccattaaatt aaggtctcat
     2821 acatatcact tctatcataa aagggagaat aaaaatgaaa caaaccacta acttgactga
     2881 agttcaagac atactgcaac agtcatccgt tgccgtcatt tatctttcga tgcctaattg
     2941 cagtgtttgt cacgctgttc gtccacgact agaggaactt ctcacacatt atgatattcc
     3001 ggcacttcat ttggatgcat ttgaaacacc agaagtagct agtaaatttg aagtgctgac
     3061 tgcgccagtt gtcctgattt ttcatcaagg aaaagaagtc tttcggcaag cacggttcat
     3121 cgactttaaa aaaatccgtt atctactaga tgaactgaca gcagaagatg attccctttc
     3181 ttatgaagaa atcttccgaa caaaataatc gttgtccaaa cagaaaaaag cgattgaaca
     3241 agtgaaagtt ttgccttcat ttgttcaatc gctttttatt tttattctgc tgtattttcg
     3301 gaatgcgcat ttgcgtcaga atgcaagaaa tagatcaagg tttgcaattc attcgtcaaa
     3361 tcaacatttt ggatcaatac atctgacgga gcattcaatc gagcagcggt aaagttcaag
     3421 attcctttga ttcctgcttc cgttaactga ttgactactt cttgtgctct ttcagcgggt
     3481 atcgttaaaa ttgctacttc gatttgttgg atcttgattt gttctgacat atcttccatt
     3541 ggataaacgg ggataccgtc tacgatgcgc ccaacgattt cctcattcac atcaaaagca
     3601 caactgatac gtatgctatt actttgatgg aatttgtatt ttagtaaagc acttcccaag
     3661 ttccctacac cgatcagcgc aacatttgtc aattgatcgt cgtttaatgt ctttgcaaaa
     3721 aagtgcataa gatcttctac atcataacca tacccacgtt ttcctaattc gccaaaataa
     3781 gaaaaatctc gacggatcgt tgcactatct acttgaacgg cttcacttaa ttcagttgaa
     3841 gacacttttt tctttccagc attatttaaa atccttaaat agcgataata caaaggcagt
     3901 cttttagctg tcgcctttgg aataatatga tctttcacga aatgacctcc acgtattgtc
     3961 taattacatc tttgatttct ccatcatagc agtatttata ggaaaaaatc aattccattg
     4021 ctcagatatt gtgaaatttt attctttatt catttattta tcatttctgc aaatctcgca
     4081 gtataatgga atttatggta gactactaat tgaaaaatag ttttttattt gaggagaatt
     4141 cacaaagtag aaaagaggat tccatgatat tattacaagc caatcagatt gctcgccatt
     4201 ttggtgcaga agtcttattt gaaaacatac atttagaaat cagtacaggc gctcgtgtcg
     4261 ctttagttgg cagaaatggc gctggaaaat cgactttatt aaagatcatt gctggaattg
     4321 aagcacctga tgaaggaaag atcgctaaaa ataaaacagc gactctcgga tacctcgctc
     4381 aggacacagg acttgattcc aatgaaacag tttggaacga gatgctgaaa gcttttgaac
     4441 aagttagaca aatggaagag cggatgcgcg agctagagat tttcattagc gaaggtacgc
     4501 cagacacacc tgcttatagt tcttatttaa aagaatacga ccggctgcaa cacgattttt
     4561 cagatcgaaa cggctatggt tatgaaaatg aaattcgttc cgtattgcat gggtttaaat
     4621 tcgattcttc attttatgat cagaaaatcg atactttatc aggcggacag cgcacacgtt
     4681 tagctttagc tcgcatgctt ttgcaacacc ctgacatttt gattctagac gaaccaacga
     4741 accatttaga tatcgagaca ctttcctggc ttgagaatta tctgcaaagc tatactggtg
     4801 cattattgat tgtttcccat gaccgttatt ttttagataa agtagtcaat gaagtctatg
     4861 aattaagcag aagaaaaatg cgccattaca aaggtaacta ttcgaaatat ctagatttga
     4921 aagcagaaca gttagcaagt gaatggaagg cttatgaaaa gcagcaaaca gaaatccata
     4981 aattagaaga cttcgttgct cggaaccttg ttcgcgcctc taccacaaaa cgagcgcaaa
     5041 gccgcagaaa gacattagaa aaaatggacc ggctggatcg tcctcaaggg gatgaaaaac
     5101 aagcaaactt tctattcagt atagaaaagc cttctggaaa tgtagtcttg caaatcgaag
     5161 atgccgctat cggatatgat aatgtcacct tatctgaacc aatcaatcta gatatccgtc
     5221 gtcaagaagc gattgctcta gtaggtccga atggtatcgg gaaatcgacg ttgctgaaat
     5281 caatcattca ccagatccct tttatccgtg gaaaagaaac gctagggaca aatgtcacca
     5341 tcggctatta cgatcaaggt caagctgatc tgcatacaaa caagacgatc ctagcagaat
     5401 tgtgggatga gcatcctacc actccagaaa aagagatccg aagtgcttta ggtgctttct
     5461 tattttctgg cgaagatgta gaaaaaccaa tccctctgct aagtggcggt gaaaaagcac
     5521 gggttgcttt agccaagtta gcgatgaaca aagaaaattt tttgattttg gatgaaccaa
     5581 caaaccattt agatattgac aacaaagaag tattagaaaa tgcattgatc gattatgacg
     5641 gcacactgct ttttgtttcc catgaccgat atttcatcaa tcggatcgcg acaaaagtcg
     5701 tcgaactctc tgaaaatggc agcaaagtct atctaggaga ctatgattat tatttagaga
     5761 aaaagcagga agaagaagaa atcgccgaat tgttggcaca agatgagcca gaatctgctc
     5821 ctgctcccac gaacaaatct gattattacc aaagcaaaga acagcaaaag cttatccgta
     5881 gccttcaacg aaaaattact caggttgaag aagaaatggc gcggatcgat gaatggatcg
     5941 atcagttgga acaagaaatg gtcgcgccag aaaacctgga agatcacgta aaactcaatg
     6001 agctgaacca agatttagaa gctgctagac aagatcaaga aacaaaattg acggaatggg
     6061 aagaactaag cttggaatta gaagaatatg aaaattaacg aaaaaaccaa aacagaaacc
     6121 attcatgctg ttttggtttt tcttttttta ttttatcaaa caaaaattcc atatttacct
     6181 ttttcaccaa taagaagacc aattactagt cattctcact aaagcttcta aataatagta
     6241 atcaccccaa atacaaggtg tatttacccc attatttttg ggcttattat aaacagactg
     6301 tgttaaaaag ccatcttctt cttccgttgt agcatatctt tctttcaatt gtcgcataat
     6361 tttccaaaca gcagactggt ataagttctt ttctgttttg tctaatagac ctgattctat
     6421 taattctaat aatccacaaa ccatgatcac catactagat gtatcttttt cttctctgct
     6481 agaatcatca aagatcaaat cccaataaca aatggtatct tctggcaacc gattcataaa
     6541 ataattggct aatattttgc ttaaatcaaa atatttttgt tctttcaaat aacgataact
     6601 taatgcaaaa ccgtaaatcc cccatgcttg gcctctagcc caacaggaat tatcactata
     6661 accttgatgc gtacttccat atcttggttt tccatctttg acatccatat aaaatgtatg
     6721 ataagttgaa ccatcttctc ttactaaata atctgctgca ttttgaatat gagcctgagc
     6781 agctttttta tatttttgat ttccagtttc ttcactcgcc caatacaata atggaagatt
     6841 catgcaacaa tccatgatca ttctaccttg ttgtgaagga tcactaggat ctccccaagc
     6901 ttgtataact cctgccttct cccaaaaacg ctgaagtaat gtttctgcag catcaattga
     6961 agcttccttc atttttcgat ttcccgttat tttataatct gctacggttg ataaagaata
     7021 caaaaaacca agatcgtgat gattagtgat aatttgacag tcaatcctgt ggataaaatc
     7081 tatatctgct ttttcagctg tttgttgaaa taaaggatca cccgtcttct caaataacaa
     7141 ccaattaatt cctgtccaaa atccttctgt ccaaccaata ttgtctctta atggataacg
     7201 tccttttata ggacactcgc ttgggaattg atactgactg ttactgatac gtttgattaa
     7261 cctctccgtg tgtctgcgac aacgctcgat ctcattattc cattcttttt ccattaattc
     7321 tttataacat tcatttatcc atttttctgg atgtattatc tcttcaaaca tcactttatc
     7381 aagcatttcc ccaccagcct ttagcataat tttagtcttt ttttattgtt tttattttgg
     7441 atagttagcc cggttgtccc atcaaaatca attttgggac gattgttcca tacttctaaa
     7501 ctataatccc gatcttgatg agcaaccact cctgttccga gccaatgggt tccttttcca
     7561 aatgtaccct ctaatccagg aataaaagtg ttttcccatg tcatcaagtt tgtattgggc
     7621 aaacccataa tagctgtgga ttttcgttta atagtatcgt cacttaaaca gataatacct
     7681 gaaaagccag cttcactcac cctatagttt tccttgtctt cttctatgat tattccttct
     7741 ttctcaggag aacattcaat agcaaaccct gtctcatata gaacatattc tgtatctaat
     7801 tctactttgt gtacgcgaat ctgccagcct tcaaaaggaa taagccaagt tctgattttt
     7861 gcctgtaaag taggttccca tgtagaggca agtacctctt tcttacatac aatgtctcgt
     7921 acattatttc gaacgatgaa ctgtccatag gttttgatgc tctcattctt aaaactggtc
     7981 gtgatatatg tgtccgctcg ctgaatccct agcattgaat ccattgccat ttctgctttc
     8041 aattgattcg ctcgcggtat cgaaaaacca aattgactag aataagcaaa ttttaaatat
     8101 ttttcattgg tcaatgtatg gaaatttggc cctaattgtc ccgcattcaa aaaaaatgta
     8161 tgtccattat catgttgtat gagcaaattg gcttctgaaa gaagtttagt tccttcacga
     8221 ataggcatag gcatttcttt agccgtccaa tactcatggt cttcaggtag cgccaacaat
     8281 aaaaaaatct tattcagcca taatggagat aacgtactat tatagggttc agtcaatgtc
     8341 aattgagggt aggtataacc aatattcagg ataccttttt catcaaatat ttctttttgc
     8401 atccaccatc ttatattccg attgactaac cccttgattt cacctaattt aaaaggcaaa
     8461 agatcggccc acaacatagc tgaccaaaag cttgaaactg caaaacgata aatcatgctt
     8521 cgaccaaaag gcacatttgc accatcttcc gcaaaaaaat gaatatagtc tctagaaaaa
     8581 tcaatagctc ggttcttata tatctggcta atgggctttt ctggttctag tacgctataa
     8641 atcaaaccat aataatgaaa tgcaaaagga ttataataat cttggcgacc tcttgaactg
     8701 tctcgatacc agccattttg aagataacaa ttttctatat tatcaaatgc ttctttcagt
     8761 gctgattctt taactggata accaaatttt tggataattg ttccgacaat tatcttaaaa
     8821 aattgccaat taccatctgc acattcatat tcaaaaattt gcgagaacca atgaataatc
     8881 tggctttttt ctcctgtatc taattttttc cataataatt gttcatgatg atataacgct
     8941 aataacattg cgggcatttc taccatacgc tggtctcttt catgtatttc tccccaataa
     9001 tcgggatggg aaggatctgt tccagctttt attcctgata gaattaattt caaccatcgt
     9061 tcatctaatt ggtcaattgc gggccccgct ccccataaaa cccgagagaa tccttccatt
     9121 cctgcaattt tatttccata tcctacccct tgatgtatat aatcaatttg tgcgccactt
     9181 gctcgataaa acggctggag tggttgattt atctttttaa ataagtcaaa gtaatcctgt
     9241 ctggtcctta atagtggtac tggatgtttc attgtatttt ctcctctttt tactaccaaa
     9301 tagctaattc cgactgttct aatcgaagta agccttcaat aaaaaaataa tcagaataga
     9361 tcagtggcac attataagtc agttctctat ggtatgcttc tgctccgcct ccaatgatcc
     9421 catcttcatc catattccaa tttgcatagt gcttttctgt cttttggagt atattgatag
     9481 cagcttcatg atacattttt gatcgtacag aggaaacata tcctgctaat tccagaagac
     9541 cgcatgcagc gcatactcca gcactggtat caatattcga atcttttaca gcaaaatcaa
     9601 tgagaggaat ataaccagtt ttttcgattt tctgaataaa atttactgct atcttttctg
     9661 ctatagctag atagtacgga ttttttgtgt aacgataact taacgcgaat ccatataaag
     9721 cccatgcatt tcctctgctc caacaagaat tctcattgta tccttgcccg ccaagtaacc
     9781 gaatatattt ccctgattct gcatcaaatt ctgcgatatg cccggttgat ccatctttcc
     9841 tcactagata cttagctgtt gtatctgcat gctgccgagc aattttagag aacctcggat
     9901 caccagtttc cctcgaagcc caatacagta atggtaaatt catcatacta tcaataatca
     9961 tccaaccagg tttatggtca ttccatgcta ctaaatagtt ccctgaaata ttatagcgtc
    10021 cagcaagtac cgtagctgca tgtaacccta gttgtcgact atgtttgttt tgagactgtt
    10081 gataatgtgc aatagccgat ggtaagaata aaaaacctac atcatgatga acgtctgaaa
    10141 aatggtacag ggcagattct aataattgtt cttgttgtat tgctgcctct ttgaactttt
    10201 ctcgttttgt cttgtgaaag agctgccata aaataccact ccaaaaacca ttggtccacc
    10261 agtcgattcc tctgtccttt attacgtctg tatattttcc gttgtaagaa atatatggaa
    10321 ttttattccc cattctgtct aattccgcat ctatcttttc agaagctaca ttcaaaattt
    10381 ttcttgacca ttccttcgtc tgttcattca tggatatccc gccattcttt taatttttat
    10441 gaatgtggag ttgctcctag aacgaactag atggttaaca ttcgttattt tttttactta
    10501 tttgcagctc caatgtcatt ctccttaaat ttagactgcg cccacacata aatcaagacc
    10561 attgggatca ctgaagttgc taaaattagc ataattgcgc cataattaat tggctcacct
    10621 gtactagaaa ccgtcatatt tttgataatt aagggaaccg tatatttatc tttatctgaa
    10681 agcatgacta atggaaatag atattgattc caacttccca taaagataag taaaccacta
    10741 gtaataatcg taggtgataa aatcggtaca gcgattttcg taaaaatcac ccactcattg
    10801 gcaccatcga ttctggcagc ctccatcagt gctgtaggaa attgactgct atattgtcgc
    10861 atcataaaca ctgaagttgt actggcaatc gaagggatga tcacacctgc ataagtattc
    10921 gataatccta aacgactcat aatgtaaaat aaaggaatca atgtggtaaa aaaaggcacc
    10981 atcctagata gcataactgt attgaacaac agtttattcc ccataaaacg atactttgct
    11041 aatgcataac ccgccattgt agaactggct gtagctgaaa ctgtactgat tacagcaata
    11101 acaatcgaat tccaaataat caagggataa ttatatctcg ccatgatggc ttgccagttt
    11161 tttaatagca tatccccaaa cttgaaattg attccgccac ttaaaatttc ttgattactt
    11221 tgcgttgaag ctacaatcat atataaatat ggcatcaaaa atattaaggc accaaacaca
    11281 ataatgacgt acgtaaaaat tctgctttgt atcttttgtt tagtcatttt tatcacctcc
    11341 gaatttaaat tgaatagccg ataatataac agatacaaac accatcgtcc aggcaatagc
    11401 agaggctttt ccaaaatcaa ccatttcaaa acttgtttta tataaattga ctcctacgac
    11461 caaagctgaa ttttctggtc cattggaagt cccaaagaat atatttggga tattgaaatt
    11521 acccaagcca ttgatagtgg ctaaaactgt ggccattaaa atgattggct ttagcatggg
    11581 gatcgttaat aaaaagaatt gccgaaattt cgatgctcca tctatttcag cactttcgta
    11641 tatctcttta gaaatccctt gaagaccagc taaaaataaa atagtgttat atcctacact
    11701 aagccaaatc atcgtgatga tcattgatat tcgtatccaa aaaggatttg ttaaccactg
    11761 tacaccgtta attcctagcg cattcaaaaa tccattgaat aacccagtcg gattgaaaaa
    11821 agtcatgaat acggaagcta cagccaccgt tgatgtaatg gttggaatat aatagacagt
    11881 tctaaaaaat ccttttcctt ttaactgtgc tgaattcaat aaaacagcaa agtaaatagc
    11941 taatagcatc gttattggaa ttgatcctgc catgataatc aaaacattcg tgtaagattt
    12001 aataaacaat ggatctcgga agatagctag ataattgtca aatccaacaa attcaaatga
    12061 gtagccaatt ttcataaaac taaaagcaaa cgagataatg atgggaatca aaatcgtcac
    12121 aataaagaaa ataacaaaag gtgtgaccaa tccataagca ctttttttat gagacgtatc
    12181 tgttgctttt ttcttgctgt tctttattgt cttgcttggt aacgaagatt gcatcatcgt
    12241 taagtcctcc taatattttt taatcaccag ttaattttac gccgtactta tgagcaaaat
    12301 tttctgcttc ttttagatat gaattttcaa aatcacctgt ttgccaatag ttatatgcag
    12361 cggtagtcaa ataagacgta gcatctgaat aagtggctgg taaaacagta tttgatgtat
    12421 tttcattgat tttattcaat agttcatagt atttttgtcc tccttagtag tcaaactttt
    12481 tctcagctgc ttctgtttga taggccaatg agttagatgt agccacgccc agctccagtg
    12541 cgttggctaa gcattcttca tctgtcagca taaatgtaag aaattgttgt gccacttgtg
    12601 gatttttggc atctttaggg acataaaaac ttgatcctcc tgatactgca ccttttcctg
    12661 gattttcttt tgaaaaagga atcagtggag ccatgcgcca tttgtcagat gcatcaggaa
    12721 aattcaaaga catattcgta gcataccagc cgccagcaac aaacattgca gattcttgaa
    12781 atgctttctc agcatcttgt gcaccgtaga aatgtacaat atctgagtcg atcatttctt
    12841 tgattaactt tgcagcatgt ttcgcttctt tagtattcag acggatattt ccttcttcat
    12901 caagcaaaga ttgattctgc tgggctagca tgttgataaa caattcttgg tccccagatt
    12961 ctggcaaggc aattccaaaa ctaccagttt tttcttttaa tgcttttgcc acctggatat
    13021 attcttccca agaactgatt tcttcaaaat ctacacctat ttcagcaaat ttttttggtt
    13081 gataataaca aagtgctggg gtaaaatcac tagcccaagg aataatcgtt tcattactcg
    13141 tttgttgacg caaaatattc aatttttgat cataaaactg atcgccatat ttgtcataaa
    13201 atccatattc atcagcacta taaaaagcat cttgaaattt ttccagcaca cctgctgctg
    13261 aagtgtctgc catggagata atatccggca tatcttcgtg tgttaccaat tttggggtga
    13321 tcatttgtac tgttgctgtt cccgaagcta cattcacaat ttttaagtca atatttgctc
    13381 cagttttgcg gttatagatt tttgcagcat tttcaacata gtttgtcagt ttgccgtttg
    13441 cgacatatac ttctaatgta gtggcttttt ttatcattgg atctgcgatg tttgctgtta
    13501 cttcttcgtt agtttttcca catgcactta agctgaataa tagggtaatg tttagcaaag
    13561 ctatcaccaa tttgtatctt tttttcatgt ctatcccttc tttcttttca agttcttata
    13621 taaaacgctt tcacttatgt atattaacgg aattagtaac acaaatattt atataaaaaa
    13681 atattttact tgtcgttttg gcttttcatt tttgttataa tagtcattaa ttataaatag
    13741 aaagcttttt atataaaaag agaaaggaaa gtggttcttt tgttaacaaa caaccaatcc
    13801 tccaatcttg tcaaaaaaac actaacctat tccatagata cccatcttca tttatctgct
    13861 gctccctatt ttcgactact aataaatgaa agcgctaatt tatatgctga atttgcacat
    13921 aaatctatag aactagataa tccttccctt ttattcattg ataaacagca agaagtccaa
    13981 atcttttcta ctcaaaagga aacacaaact tttatcatta tccagtttta ttttgatgag
    14041 caattattag cacatattac gggacaatct ttttctatag atcaagaaat aacatctatc
    14101 attacttttc tgaaaaatga acagttcaaa attgattatc taaaacatag taagccagct
    14161 atttcctatg atcaggaaat caattctttt ttcatactat gccaacagat gagcattcat
    14221 ttgctttcct tattagttac ttattgtctg caaaaaattc aattgtcttc cttaccaagg
    14281 cattatcaac aggaacttgc tttattgaca ataccttcaa atgagaaagt cattcctaaa
    14341 aaaaagcata ttctttattc agatgaatta gttgatccaa tcatacgata tttagaagaa
    14401 catctttcag aaaatttttt gcttgaacaa ttagcccaaa ttttttttat cagtccagcc
    14461 agcttaaaaa aacattttag aaaagtgaat ggaaaaagtg ttattagcta ttttaaagaa
    14521 ctaaaaatgg aaaaagcaaa acgtctcgtg ctagaaaatg aattaaccta tacagaaatt
    14581 gcagatatat taggatttca ttcgatccat catttttctt cagcttttaa aaaatataca
    14641 gggttatccc cttcaaagta ttatctatcc ttaaaataga aaaaaatttt taggttttat
    14701 ccacaaatta tttctctata ttttctgtct attcaaaata aacaataatt ttccggcaca
    14761 attatttgct gttttttacc ataacatagt acgatcaacg tagaaaagga gatgttttta
    14821 tgaaagttgt tgtcattggc ggtgtagctg gcggtccttc atttgccact cgtttccgtc
    14881 gattgaatga agcgcacgaa atcattatct atgaacgcgg agaaaatatt tcttatgcaa
    14941 gttgtgcttt gccttattat ttaggaggtg tgatcactga ccgtgactcg ctgatcgaac
    15001 gtacaccaga aatattgaaa acaaaaaaca acatcgatgt ctttactaaa cacgaagtaa
    15061 cagcaatcga tccttctact aagcgattaa cagttaaaga cctatccaca aatgaagaaa
    15121 taaaaacaga ttacgataag ttgatcatct cttctggtgc tagaccagat tatccggata
    15181 ttcccggagt ttttgaagca gaaaacggct ttgtcctccg tagtgtgacg gatgcggatc
    15241 gaatcaaatc gttccttgaa gaaaaaaatc cacaacatgt cgtcattctt ggtgcaggtg
    15301 ttatgggtct ggaattagct gagaatttca agcatcgcgg cttagacgtg actttaatcg
    15361 atcaattgcc acaagtcgct ttcccttatg atccagaaat tgctaattta gtttatgaca
    15421 aattgctgaa agaaggatta gccgttcatt tagaaacaag agttactgag atccgtgata
    15481 aaggtcgaga aatagtatta tcagatggct ccgtcctttc tgctgatatg ctaatttttg
    15541 ctgttggtgt ttctccgaag aatgaagtgg tgaaagcaac cggcatacaa ttatcagata
    15601 caggtcagat cattgttgat gaccagttac aaaccaatct gccggacatc tatgcgattg
    15661 gcgatattat cgaaacgact agtgtagtga ctggtcagcc gatccaaagt atgctttcca
    15721 gtgcggccaa tcgtcaagga catatgctgg cagatatttt aaatggtacg cctatgcgct
    15781 atcgcggata tattggtgca ggtgtcgcaa aaatctttga tcatacagca agttatgcgg
    15841 gaatgacaga acatgaatta aaagcatcag gcataacaaa ttataaaact gtttttatca
    15901 ctccctttga ccatgcctat ttctatccag aagctacaag attaaatcta aagctgattt
    15961 ttgatgcaga tagcggtcgt attttaggtg gacaagcatt tggagaaaaa ggtgtcgata
    16021 aacggatggg agaactttct gtagcaatca ccggaaactt gacagtcttt gatttgcctg
    16081 atttggagtt gccttactct ccaccgtatt ctactacccg tgacccgttg aatatagctg
    16141 gttatgtcgc aatcaatcaa atgacgaata tcgtagaaac gatcaaagca agtgatatac
    16201 ccgaaaacga tttgaaagaa gcgttctttt tagatatacg tgaacctaat aaagcacctt
    16261 ctggcagtat ctcggcaaca aaaaatattc caatgaatga gttacgagat cgaatcaatg
    16321 aaatcccaaa agataaaaaa gtttacatta ctttcagaag aggattgaat acttatactt
    16381 ctgcccgaat cttggcaggt ttgggtatca aagcgatttt gattgaagaa taaaaaaacg
    16441 accgcggtgg gtagccgcgg tctaggagtt atttttactt gaggagtaaa aatgaaaaag
    16501 tatatagggt tgttggttgg tatgtatata gaatagaaga taaatgtgaa gaaattgtgc
    16561 attatttctt cacatttatt aggttatagt ttaagaaagt ttaaacattg cgcaatttaa
    16621 ttattttagc tgtttctttt cataaaaaaa catgttgtga ctctgagaca aatcatagtc
    16681 catagtgatg tctctagagt aactacgcct aatattacga ggcatcatat taatctatca
    16741 gcactagtac ctaaactagc atccctttcg aatatttttt cttatataat aaagtttgct
    16801 ttcactcctc taatctcacc aaatcgactg gcttgcgttg tgtgatacta ggaactgatt
    16861 ggtttagaac gacttttcta ttgtaatcat atttttcgcc atcctcgttg tcttctaatc
    16921 cttcaatgaa tgatccctaa tgtgtgtatt aaataattta atttttttgt tcctatttat
    16981 ttaaca
//
LOCUS       DACDEG010000050        16639 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.052, whole genome shotgun sequence.
ACCESSION   DACDEG010000050 DACDEG010000000
VERSION     DACDEG010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.052"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(63..701)
                     /gene="plsY"
                     /locus_tag="IV142_001705"
     CDS             complement(63..701)
                     /gene="plsY"
                     /locus_tag="IV142_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP8485365.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            828..1262
                     /locus_tag="IV142_001706"
     CDS             828..1262
                     /locus_tag="IV142_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP8485366.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            1428..3473
                     /gene="parE"
                     /locus_tag="IV142_001707"
     CDS             1428..3473
                     /gene="parE"
                     /locus_tag="IV142_001707"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP8485367.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            3485..5935
                     /gene="parC"
                     /locus_tag="IV142_001708"
     CDS             3485..5935
                     /gene="parC"
                     /locus_tag="IV142_001708"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP8485368.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            6212..8443
                     /gene="pflB"
                     /locus_tag="IV142_001709"
     CDS             6212..8443
                     /gene="pflB"
                     /locus_tag="IV142_001709"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP8485369.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            8687..9448
                     /gene="pflA"
                     /locus_tag="IV142_001710"
     CDS             8687..9448
                     /gene="pflA"
                     /locus_tag="IV142_001710"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP8485370.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            9533..10459
                     /locus_tag="IV142_001711"
     CDS             9533..10459
                     /locus_tag="IV142_001711"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP8485371.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            10631..11524
                     /locus_tag="IV142_001712"
     CDS             10631..11524
                     /locus_tag="IV142_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP8485372.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            11658..11783
                     /locus_tag="IV142_001713"
     CDS             11658..11783
                     /locus_tag="IV142_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8485373.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(11917..12549)
                     /locus_tag="IV142_001714"
     CDS             complement(11917..12549)
                     /locus_tag="IV142_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485374.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(12697..13239)
                     /locus_tag="IV142_001715"
     CDS             complement(12697..13239)
                     /locus_tag="IV142_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP8485375.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13374..13763
                     /locus_tag="IV142_001716"
     CDS             13374..13763
                     /locus_tag="IV142_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485376.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            13767..14531
                     /locus_tag="IV142_001717"
     CDS             13767..14531
                     /locus_tag="IV142_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8485377.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            14660..15607
                     /locus_tag="IV142_001718"
     CDS             14660..15607
                     /locus_tag="IV142_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8485378.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            15737..16477
                     /locus_tag="IV142_001719"
     CDS             15737..16477
                     /locus_tag="IV142_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485379.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         5876 a         2812 c         3456 g         4495 t
ORIGIN      
        1 aataagggag tgtgacatcg atttgtcaca ctcccgtttt atctctttgg aatgaaaaag
       61 tatcaggatt ttttcttgct gctattcaaa ccaaacggga ttcgactttc agtatggttt
      121 ctgatacgct tgatattgtc tttatgccga taaaagataa aggaagaaac taaaaaggca
      181 atgatcgtca gtaaccaatt gggcttagcc aaaatcacag gagctacata aggaaggacg
      241 atcgtagata aagtgatcaa tactgcactg atcatactgc tcaaactcac catacttgta
      301 caaaataaac taacgataaa gatgatacaa gaataaacaa aaaatacagg attaaatcct
      361 aaaagcattc ctgcacttgt cgcaacagct tttcctcctt tgaacccagc aaaaatcgga
      421 aatgtatggc caagaatggc gcatacccca aaccaaagag ggttaatccc agaaaggtgg
      481 aagatcatcg gcaaacaagt agccaatgta ccttttaaaa tatccataag caggacaaca
      541 atcccggctg ttttgcctaa tacacgaaat gtattggtcg ttccagtatt accgctcccg
      601 tgttgtcgga tatctttttt gaaaaacaat ttgccaatcc agacaccgga aggaatagaa
      661 cctaaaagat atgctaaaat aagtaataag atcaatttca ttttttaccc tctttcaagt
      721 tgaaaactat cctttatttt agcatgaata ggtaacagga acaattgaaa aaatgaaaga
      781 tacaagtata atagatggaa taaaaagaaa agcaaggggt ggcagaaatg tctgtaaaaa
      841 atccaacaca agaaacgatt ttcaactatt tggaagaagc aaaaaatatt gccgtcgtag
      901 gcttgagcaa taaaccagaa cgaacaagtt acatggttgc acaggtattg caagacaatg
      961 gatataaaat cataccagta aatccattat tggctggtga agaaattcta ggcgagaaag
     1021 tctatgcgaa tttgacagaa atcgatgatc caatcgatat cgtagatgta tttagacgga
     1081 gtgaattttt acctgaaata gcaggtgatt ttttgaaaac tgatgctaaa gtgttctggg
     1141 cacaattagg gattgaaaac aaagaagcag ctgagatgct aagaaaagca ggacgtacag
     1201 atatcgtaat ggatcgttgt attaagattg aacttggtaa gttgaaagaa aaccaaaaat
     1261 aaaaatgacc acaaagaaga gatgatcgat agaggggaat gtcgaaaaag cggttaggag
     1321 ataattaaaa ttgatttctc gtctaccctc ttttttttca cgaccttttc tagtatgata
     1381 atcgtggtgc ggttttgccg caaagtatta ttgaaggagc gtttgccttg gcaaaaaaag
     1441 taaacaacga atacaatgac tcgtccatcc aagtattgga aggattagag gcagttagaa
     1501 aaagaccagg gatgtacatt ggatccactg acagccgagg cttgcatcat ttagtctatg
     1561 aaatcgtcga taatgcagtt gacgaagcat tatcaggtta tggcagtgag attgatgtga
     1621 ccatccatga agacaatagt ataactgttg cagatagcgg acgtgggatg cctgtaggaa
     1681 tgcatgcttc cggaatacca acagttgaag tgatttttac tgtgctgcat gctggcggaa
     1741 agtttggtca aggagggtac aaaacttctg gtggactgca cggtgtagga gccagtgtgg
     1801 taaatgcctt atcgaaatgg ctaattgtaa cgatcgttag agatggcgta gagtatcagc
     1861 agaaatttaa aaacggcggt aaacctgacg gtactttgaa aaagatagga aaaacaaaaa
     1921 aagcaaacgg gacgactgtc cattttttac cagatgatac aatattcagt acaacgaaat
     1981 tttcatatga aattttagcc gaacggttaa gagaatctgc ctttcttcta aaaggcgtta
     2041 aaatctctct ttctgatctt cgtggagaag aaccagtcaa ggaaattttt cattacgaag
     2101 aaggaatcaa agaatttgtt gattatctga atgaagaaaa agatacattg acaccagttg
     2161 tttatttttc aggagaaaaa gaaggtatcg aagtagaggt cgcttatcaa tacaatgacg
     2221 gttattcaga aaatgtttta tcttttgtca ataacgttcg gacaaaagat gggggaaccc
     2281 atgaagctgg tatgaaagcg gcgatgacga aatcttataa tgaatatgcg agaaaagttg
     2341 gtttgctgaa agaacgggac aagaatctgg aaggtagtga tttcagagaa gggttagctg
     2401 ctgtactttc tatccgtgta cctgaaaatc ttctgcaatt tgaagggcaa acaaaagaaa
     2461 aacttggtac gccagttgct cgtaccgtag tagataacgt catcagtgaa caaatgggct
     2521 tttatttgca ggaaaatagc gaaatgagcc agatgttggt ccgtaaagca atcaaagcca
     2581 gagaagcgag agaagcagca cgtaaagcca gagaagaaag ccgaaatggg aagaagcgta
     2641 aaaaaggcga atctcttctt tcaggaaaat tgacacccgc acaatcccga aaccctaaga
     2701 aaaatgaatt gtatttagtc gaaggggatt ctgctggcgg atctgctaaa caaggaagag
     2761 acagaaagtt ccaagcaatt ctacctttac gaggaaaagt catcaatacc gaaaaagcta
     2821 aaatgcaaga tatcttgaaa aatgaagaaa tcaatacgat gatctatacg attggtgcag
     2881 gagtcggtcc tgagttttct atcgaagact gtaattacga caaagtaatc atcatgaccg
     2941 atgcggatac agacggcgcc catattcaag tattactgct tactttcttt tatcgttata
     3001 tgaaaccgct gattgaagct gggaaagtat atatcgcgtt accgccttta tataaagtat
     3061 ccaaaggaca aggaaagaag caagtcattg aatatgcgtg gacagatgac gaattggcag
     3121 caatgattaa aaaagtcggc aaaggatata tgctgcagcg atataaagga cttggagaga
     3181 tgaatgcgga acaattgtgg gaaacaacga tggatccgac ttcccgtacg ttgatccgcg
     3241 tccgtatcga tgatgctgcg caagctgaac gtcgagtaac cacactgatg ggtgacaaag
     3301 tagaacctcg aagaaaatgg atcgaaaatc acgtgcaatt tacgctcgaa gaagatggca
     3361 gtatcttaga taaaaaagag gatactgaaa tctctccttc agtttctaat gatttattag
     3421 atgaagaacg agcagataaa aatgaaaaca atcagttatt tgaagtagaa taggaggtca
     3481 cctgatggaa aatcgacaag aagtacaaga attaacttta gaagaagtga tgggtgaccg
     3541 cttcggacgt tattccaaat atattatcca agaacgtgca ctgcctgata tccgtgatgg
     3601 attgaagcca gtgcaacgcc ggatattatt tgccatgaac aaagatggca atacgtatga
     3661 caaaggattc cgtaaatcag caaaatctgt cggaaatatc atggggaatt atcatcctca
     3721 tggtgacatc agtatttatg aagcgatggt ccgcatgagc caagactgga agttgcggga
     3781 agtactgatc gaaatgcacg ggaataacgg gagtatggac ggtgatccac ctgctgcgat
     3841 gcgttatact gaagccagat tatccaagtt aagtggagag atgctagcag atatcgagaa
     3901 agaaacagtc gatctcgtgt ggaattttga tgatacagaa aaagaaccaa cagtccttcc
     3961 agctcgatac ccaaaccttc tcgtcaatgg atcgacaggt atatctgctg gatatgcaac
     4021 agaaatcccg actcataact tagctgaagt gatcgatggc acaatctata tgatcgatca
     4081 tccacaagcg agccttgaaa aattgatgga atatattcct ggacctgact ttccaacagg
     4141 agggatcttg caaggaaaag atgaaatcaa aaaagcttat gaaacaggac gaggaaaagt
     4201 tattcttcgt tcgaaaacaa agatagaggc aatgaagggt aataaacagc aaatcgtgat
     4261 cacagaaatc ccatatgaag taaataaagc gaccttggtg aaaaaaatgg atgagatccg
     4321 tttgaataag aaaatcgatg gaatagctga agtacgagat gaaagtgata gaacaggtct
     4381 gcaaattgtc gtcgaattga aaaaagacgc caatgctcaa ggaattttga attatttgtt
     4441 caaaaacact gagctacaga tcaattacaa tttcaatatg gtcgctatcg accatatgac
     4501 tccgcatcaa gttggactga aagacatttt aagaagttat atcgagcatc gaaagcaagt
     4561 cataacaaaa cgtagtcaat tcgatttagc caaagctcaa aaaagacagc atatcgtaga
     4621 aggattgatg aaagctttat cgattctgga tgaagtaatc gcaacgattc gtgaaagtaa
     4681 agacaaaaaa gacgccaaaa agaatttagt cgacgtcttt caatttacag aagaacaagc
     4741 agaagcaatt gtcacattgc aattataccg tttgacgaac acggatatca cagagcttca
     4801 aaaggaatcc gaaagcttga ttgcgcaaat cacagagttg aataaaatcc tttcaaatga
     4861 caaagagctt ttctctgtta tgaaaaaaga attgcgagaa gtgaaaaaaa attattcttc
     4921 cgctcgtcta acgacaatcg aagacgaaat cgaagaaatc aaaattgata cgcaagtctt
     4981 agtggcacaa gaagacgtaa ttgtcagtgt gacgcgagaa ggctatgtga agcgaacaag
     5041 ccttcgttct tatagtgctt ccaaaccaga agaaatcggg atgcgagaag gcgactactt
     5101 gctatactca ggtgaattga gtacattgga ccatgttctt ctgatcacaa ataaagcaaa
     5161 tgtgatctac cgccctgtcc atgaactacc cgatttgaaa tggaaagatg ctggtgagca
     5221 catttctcaa acaatctcca atctgtctgt agacgaatcg attttagctg ttttcccata
     5281 tcaaaaaatt caagcagaga aaacatttgt attcatcagc aagaatggat tgatcaagca
     5341 aacacgaatg acagagttcg aaccatggcg cacctataag agccgtccgc tcagctgtat
     5401 gaagctaaag gcggaagaag atgaactgat tgctgtgtat ctgaaagaag atcagccagg
     5461 cttagatgtt ttcttagtaa cgcatcaagg aatgggtttg cgctatcctt taacagatgt
     5521 tcccgttgtt ggagcaaagg cagctggagt gaagtctatg aatttgaaag atggagatag
     5581 gattatcaac gggctgcttg tgttagcaga aggtgatacg ccgatcgtaa tcgttactca
     5641 gcgaggagct gttaaacgca tgcttgctca agaaatcagc caaacttcac gtgcgaagcg
     5701 gggagtgacg gtcatgcgtg agctgaaaaa aaatccgcac cgtattatct atatgtcgga
     5761 aggaagatca aaagaaataa ccttgatgaa tcaaaaaggg cagcagttaa cgattgaccc
     5821 aactgatttc cctataggtg atcgaacatc gaatggatca tttgcgatgg atgagaaaaa
     5881 aggtggagaa gtcatcgaag ttatcgatgc tcctgagatt ttcatcgagt cgtaactgtt
     5941 gtagtatttt aatgaagaga cgccatctgt attaatacag gtggcgtctc tttttcattc
     6001 atttttaata aatccttata cgtttgcaaa aaaagatgaa agtgttcaca atgcttaaat
     6061 gttggtatgt caacgttgat ttaaatatat gtaaataaaa cataaaaatt gttaaataaa
     6121 aaacaagaaa acccttgcaa agatagggtg ctgtgttata ctaatcacgt aaaaactgtt
     6181 acataaaaca gaaatttagg aggtccatac aatggagcaa tggaatggat ttaaaggtac
     6241 caaatggaaa gaaggcgtag acactagaga ctttattcaa aataactaca ctgaatatcg
     6301 cggtgatgac agctttttgg aaggcccaac agaagcaaca gaaaaattat gggatgaatt
     6361 gcaaaagtta aatgatatcc aattcgagcg taacggtgtc tatgatatgg acacagaaat
     6421 cgtatcaact attacttcac acgatgcagg ctatttggat aaagatttgg aaaaaatcgt
     6481 cggtctacag acagacaaac ctttgaaaca agcattcatg ccgttcggcg gaatcaatat
     6541 ggctaacaat gcgctaaaaa gcaatggcta tacaccaaac gatgaattga cacatatttt
     6601 cactgactgg cgcaaaacac ataatcaagg tgtatttgac gcttatactg ctgaaatgcg
     6661 tgctgctcgt aaaaacaaaa tcattacagg gcttccagac gcttacggtc gcggacgtat
     6721 catcggtgac tatcgtcgtg tggcattgta tggaatcgac tatttgatgg aacaaaagaa
     6781 aaaagatcat gatatgactg gttatcatac aatgagtgaa gaagtgattc gtttacgtga
     6841 agaaatcact gaacaatatc gtgcattagc gcaaatgaaa gaaatggctg cgaaatatgg
     6901 ctatgatatt tctaaaccag ctgcaaatgc aaaagaagct gtccaatggc tatacttcgg
     6961 atatttagca gcaatcaaat cacaaaatgg tgctgcgatg tcaatcggac gtatctcagc
     7021 attccttgat atctatatcc aacgtgattt ggataatgga gtaatcacag aaaaagaagc
     7081 acaagaattg atcgaccact tagtaatgaa attacgtatg gtcaaattcg cgcgtacacc
     7141 tgaatataac caattattct caggattccc tatctgggct actttatcca ttgcaggtat
     7201 gggattagat ggtcgttcat tagttacaaa gaatgatttc cgtctgcttc atacattaac
     7261 aaatatggga ccttcaccag aaccaaactt gactgttttg tattctgaac accttccaga
     7321 aggattcaga acatatgcta gcagaatcgc tatcgaaagt tcttcgattc aattcgaaaa
     7381 tgataatcag cttcgtgaac aatggggttc tgatgattgt gcgattgctt gctgtgtatc
     7441 aggaacagtt gtcggaaaag acatgcaatt ctttggcgct cgtgctaact tggctaaaac
     7501 actattatat gccatcaatg gcggagtcga tgaagtgact ggcgcccaag ttggaccaga
     7561 atatcgtcca atcacatcag aaatactaga ctttgaagaa gtgaaacata acttcatgaa
     7621 tatgatggat tggttagcag aactatatgt caatacgttg aacatcattc attatatgca
     7681 tgataaatat tcttatgaag cagctcagct ggctttgatg gattctgact tgcaacgcac
     7741 atttgctaca ggtattgctg ggatctctca tgcggcagac agcttgtcag cgatcaaata
     7801 tgcaaaagta aaagcaatcc gtaatgaaaa aggaatagca gttgattttg aagtggatgg
     7861 cgacttccct aaatacggaa atgatgatga ccgtgcagat gaattagttt catggatctt
     7921 ggaatacttc atgggacaaa tcaaacgtca taaaacttac cgcaactcaa aaccaacaac
     7981 ttcattattg acgattactt caaatgttgt ttacggtaaa aatacaggaa atactcctga
     8041 tggccgtgaa gctggaaaac cacttgcgcc aggagcaaat ccaagttaca atgcggaaca
     8101 aagtgggtta ttagctagcc tgaactcaac agctaaaatg aattacggct gtgcattgga
     8161 tggaatttca aatacccaaa caatcaatcc ttcaggtctt ggaaaagaac aagatacaag
     8221 aatcgaaaac ttgcgtaatg tcttagatgg atacttctca aaaggagctt atcatttgaa
     8281 tgtcaatgta ttcagtaatg atttattgcg tgatgcaatg gaaaatccaa tgaaatatcc
     8341 gaacttgacg atccgggtat ctggttatgc agttaaattc cgtgacttga caagagaaca
     8401 acaattagat gtcttgatga gaacttcaca cgacagaatg taaaaagttc gtgaaaaaag
     8461 tttttcgact tagcgtcgaa aggacgttga atagccatcg catcaaaatc gaataatcag
     8521 tatgcttatt tgaaagaaac gatttacagg ggcaggactt gtatgttccg tccctgtaaa
     8581 ttttacacat aacgaagcgg caattgcgaa aaattttgtt cagaaagctt cgcttcgaca
     8641 gctaacttgt acttaaaatg aaatcaaaga aaagatgtga acttaaatgg aagaaaaaac
     8701 aatcggctat gttcattcta tcgaaacttt cggttcagta gacggacctg gactgcgctt
     8761 tgtcgtgttt atgcaaggat gccgcatgcg ttgccaattt tgtcacaatc cagatacttg
     8821 gaatattggc ggagggaaag aatacacagc agatgaactt ttagataaag cagaacgctt
     8881 cagaccttac tggggagata aaggtggtat tactgttagt ggtggagaac ctctattgca
     8941 gatcgatttt ttgatcgaac tatttaaaaa agcaaaagag cggaagatgc atactacatt
     9001 ggatacatgc ggaaaacctt ttacgtatga agaacctttc ttttcacgtt ttcaagaatt
     9061 gatgaagtat acagatttac tgttatttga catcaaacac atcgataatg aagagcataa
     9121 aaaactaacg cattgggaca atgaaaatat tttagaaatg gctcaatatt tgtcaaaaat
     9181 caacaaaccg gtttggattc gccatgtact agttcctgag cgaagtgatt atgatgaata
     9241 tttgatccga ctagacaact ttattcaaac cttgtcaaat gtagacagag tagagattct
     9301 tccttatcat acgatgggaa aatacaagtg ggaaacactt ggtttgaaat atccattaga
     9361 agggatcgaa ccacctacaa aagaacgtgt cgaaaatgct aaacgattgc ttcatacaga
     9421 agagtataca aaatatttaa cgcgataggt cgcatcggaa agaagataag tattgcttct
     9481 ttccgttttt ttgctattat taaataggag aaatcacaaa ggagcgaatg acatgtcaaa
     9541 aattttagtt tttggtcacc aaaatccaga tacagatgca atcggggcag ccattgcgtt
     9601 ttcacacttg caaaatgaat taggtaaaga aacagaagca gttgctctag gtacacctaa
     9661 tgaagaaaca caatacgcat tagaccactt tcagctatct gcaccacgag tagtagagtc
     9721 agctaaagca gaaaatgcag atgtcatgct tgtggatcat aatgaattcc aacaaagtat
     9781 ttcagatatc agcgaagtaa atattttagc tgtagtagac caccatcgta ttgcaaactt
     9841 tgaaacagct aatccattgt actatcgtgc agaaccagtt ggatgtacaa gtacgattat
     9901 tttaaaacta tataaagaaa accaagtaac aattcctaaa gaaatcgcag gaatcatgct
     9961 ctcagctatc atttcagata cattgttgtt caaatcacca acatgtacgc ctgaagatga
    10021 agcagctgca aaagaacttg cagcaatcgc tgaagtcgat ctaaacactt atggacttga
    10081 aatgttgaaa gcaggaacaa atctaagtga taaatcagct gcggttcttc ttgatttgga
    10141 tgcaaaaagt ttcccaatgg gcagttccaa cgttcgtatc gctcaagtca atacagtgga
    10201 ccttaatgaa gtgatggatc gacaagcaga gctagaagca gcaatggaag atgaaatgaa
    10261 agcaaatggc tacgatctgt tcgtattagt tgtcacaaac atcttagaca gtgattcaga
    10321 attacttgtt gctggtaaag gaaaagaaaa agtagaagaa gctttccaaa caactttgac
    10381 caacaaccgt gctttgctta aaggtgtcgt ttcacgtaaa aaacaagtcg tacctcaatt
    10441 gacggctgta tttagctaat caaatggtag tatgaagcat tcaaaatgaa tggataagcg
    10501 attgatataa aaagaaacat agaacatact aaaggccgaa gaaatggagg agaataacag
    10561 tcaattatga ctcttgttct tcctcctttt tactttaagt aaaataaaac ggatagaaaa
    10621 gagaacatat atgaactatt attcaaaaaa taaggaaaac ttttatcaaa aactgaccga
    10681 tgatccgctt ttttcttcat tgaccgatta tctatatgaa catcgagaac aagaaacgat
    10741 tctaagagaa ttgaaaaaag aattttcgca aaataaattt tcccattttc ttgatttatt
    10801 gatcgatgca ggattaatca agagggaaga acgaagatat catttgaact ttccaatatt
    10861 tgatcctaaa gattacttgc aacaagccac ttcggctgct gaaaccatag ctgaacaatt
    10921 gaagagactc tctgttgatg aacaaaaatt agcgatggga gaagttattt gggcgtattg
    10981 tttcgaagat gaacggaaag aagcgtattt ttatggagta agaaacagcc gagagacaga
    11041 attgctgagg acaactgcag gaaatgagaa gtaccgtttt atcacacttt cctctataga
    11101 acacttcccg cttactttgg ctaattactt ttttgtacag aagaatcagc ttcctgtaac
    11161 gaaggcattc aaagaattag ccgaacttat tggagacgta aatgaatcgt acttttttga
    11221 tcaaatagaa gttatcgttg acaggatacg taagaataaa tataaaaatc gccggcctag
    11281 tattttccat cagtctctac ttgtgacgaa tactataaag gaagaagaat cattcacatt
    11341 agaattacct attgtcgaaa aaaacaattt tgagattgaa ttaccaacat tggatccttc
    11401 attgacaatg gaggaaatgg cctttcttaa acgacaaatc ttttctgagc tgagcaagaa
    11461 atttataccg catgcatttt cttatatcaa agaatatgga acagttttag tgagtaaaac
    11521 ctaagagagc agtaagcaaa aattatactg actcttgcag gaggagcctt tctcttcctt
    11581 tatggttaaa agaattattt gagtattttg cctatctacc acctattaga ttcactacat
    11641 ggtctggaaa caaaggaatg caaaaaaatg gtgagaaatg tggaatgaca aaggaagtag
    11701 tgataagaaa agttagattt ttaaataatc aatattatga ttcagtcaag tatggcattt
    11761 tatgggaaga actagctgat tagattagga agtccatttg ataatccaga atcactaaga
    11821 aacgaatggc gagtcattaa taatttatcc aatcttgggt tattctttta gattgaagtg
    11881 tttaaaacta aaaaagcatt tatgaagtcg taatagtcag gcttcaaaat gctttttatt
    11941 ttgtttgttt tttctttgcc acataaatac acctagactg acgatgaaga gcatatccaa
    12001 cagatttcct gcgacttgga tcgtcgaagg gttggtcagt ggcattaaag caaggacaaa
    12061 aaaggaaaac aagctgtaaa taaaacaggt gacaggaact aatcgaaaga agagcggaca
    12121 tttcaattgg ctaaaaatga gtgaaaaaat aaaataagta aaaatggata aaatcacgac
    12181 taaaccattc atgataaaga agaaaagcgt attgtgatta aacgaataaa aaagaattgc
    12241 taaaaaagaa aacgttttaa atgcaatcaa tacgcttgaa gcttcgttta acgtaccagg
    12301 atgtcgatga aaaaaatagg caccgaaata aacagaaaac agtaagccgc aaagagaaga
    12361 caaaatcaaa aaaaacggat tttcttcggc ttctgtgata tcagaatgat ccaaaagcaa
    12421 ccagcctaat gcactagaag gcgcatgtga ggtcttaagg tatgaataac atagaaaaaa
    12481 taagatcgtc aaagttgcaa aaaaagagcc taaatgtaaa aaattagtgg ttctacgttg
    12541 aatttccata atatctccaa attaaattga taagtatata aagtataata acaaattgat
    12601 gagtatatgt cgatgttttt taatcaaaat aataaataaa aactgtctaa catttaaaat
    12661 ttaatttaga tattagacag tttttaagtt tgtgagttat tttccttgtc gttttctcac
    12721 ttttttagtc cagaaaccac ctgaaatata ttttggctca taagaaatga taaaagcacg
    12781 ctcttcgatt tctttgatca ggcgatacag ttcacgttcg ctttttctag gagaaagtat
    12841 ttcgagaact agccgctcac cttccagccc cattgcagaa gtctgtgtca caccataacc
    12901 gtgttctctc agtatacccg gcaaactttt atcttctgcg ctatttgctg gcaaaatcgt
    12961 tgtgaccatg atgtatccta atgccaattt gtcttcaatc aaaattccga cactgatacc
    13021 tactgcgtaa cctaacgcat agacaaataa attcagtgga ttatctaaac gatctaaaac
    13081 aagggataag ccgacgatat aaatcgtgat ctcgaccata ctgacaagtg gagcaatcag
    13141 tcgatagcct ttcattgtca acatgaaacg taacgtgttc agagtgatat aagcaaaatt
    13201 aatgaggaag atcatcccca acatttttgg atcaatcatt tgtaaaactc ctttcattta
    13261 tataacaaca atatcaaaaa aaatcgtaag tgaagaaaaa ttgaaacttt atacatacaa
    13321 aaaaatgata aaatgattat aatagaatta taaagatagg ttaggtgatt aaaatgaaaa
    13381 cggtcaatcg tgtgttttgt gccagccacg tgctaagtct ccttctcagt atatacttag
    13441 gtgtctattc gattcaacag ccaattgagg tagagaacat tgtttttcag atgtcgctga
    13501 cagatggttt aattttaagc gtttttttct ttttgatact gtttattggt aatattttcg
    13561 gtagcgttgc cagttttttg aatttttcta tttatccact cctttcgcta gcattaggag
    13621 tggtgggact ttgttctctg tgcttatttc ccgagccatt cagtcctgca tttatattat
    13681 ttggtacact gaacctgttc cagacaactg ttggagcatg gttgctatgg cgatcaggaa
    13741 atttaatgaa aataggtgag taacaattga atccaattat attgaaacgt tctgtccatc
    13801 atattccagt gttagaagtt gttctgaaag aattaagaga ggaacgtatt cccgttgtca
    13861 tctattatca cggatggcag acaagtaagg agttagtttt gacgcaagga agaaaaattg
    13921 cgcagcaggg cattcgtgtg atcctgccag atgcgatgaa tcatggggag cgcaaacagc
    13981 cggtttcttc tattccatcc tatacatttt gggatagtat ctacggaaat ctgtttgaat
    14041 ttgacacact tatcaagcat ttagaaaaaa gaggtcttct acatgatcag cttgcagttg
    14101 gcggggtttc gatgggggga atgacaacat gcgccttgtt ggctaaacat cctcatatag
    14161 tagcgggcgt gtgtttgatg ggttcccctg cacctttgga atatggaaaa gaaatcgtaa
    14221 ggcgcgcatc ggaatataat tatgtcgtac caaaagacta ttttgatttg atttcatggg
    14281 ttaagagcta tgatttgtca ttgcagccgg aaaagttagc tggacgtcct ctgttctttt
    14341 ggcatggaca agctgacgaa aaaatccctt atatacaaac ggaacgattt gtagaaaaaa
    14401 atcggcataa gtcttacgga aaagaaattg tttttcgttc ctcaaaaaca cagcgacata
    14461 tggtacaaat accactaatg gaagaagctt ccactttttt agcaaaaaat ttgataaaaa
    14521 agtataaata attgaaacgg aaaatcactc atgacgatag aggattttcc gttttttatt
    14581 ttgggctata ataaactctg ttaggaaaat ttggtataat tgatagtaag aatgacaaca
    14641 tgttagaaag gaagagagca tgcccacttt agcagatgtt gcaaaacgtg ccaatgtttc
    14701 aaaaatgacg gtctctcgag tcatcaatca tccggaacag gtgactgacg aattgaaaga
    14761 gttagttttc tcagcaatgg ctgaactgga ttaccgtcca aatattgctg caaaagcact
    14821 tgtcacgaat cggtcgcaga tcatcaaatt atttatactg gaagaaatcg acacaactga
    14881 gccctattac atgaatttgc tgatgggaat cgctaaacgg atcggtaaag cgcattattc
    14941 tctccaatta gttacaaatg atgccttcga cgtcggttct tgtgatggat acatcatcac
    15001 aggtgtcaga aaaaaagact tcgattggat cgaccgtttg gaaaaaccag ttgtcttgtt
    15061 tggggaaaat tctgaaggat atgattatgt agacagcgat aatgaatatg gtacagcaaa
    15121 agcaacgcaa tatgccttag aaagagatta tcaaacgatt atttatatcg gtatcgaagt
    15181 ggacgagcct tttgaaattt caagggaaac tggttattta tctgtgatga aggaagttga
    15241 tcttcctagt aaaatcttgc ggtttccaaa tcattcgaca gaagtagaaa gatatatgac
    15301 agaaaactgg gcagatttta cagataaaac ttgttttgta tgtagttcag atcggctagc
    15361 actaggagca gtaagaggga ttataaatgc tggaggacac ttgcccgaag aatttggtgt
    15421 gattggattt gatggggtat ttttagacca aatcagttca cctaaattga caacgatcaa
    15481 gcaagatatt atacgtatgg gtgaagtatg tggagaaatg ttgttaaaaa aaatagaaga
    15541 aaaaggggcg agtcaaggtt atcgtcgttt ttttcctgaa ctagttatca gagagacaac
    15601 gaattaagtc ttattactat atatatattt atgaaattca tcgctatttt cttaaaataa
    15661 tcagcgcatt gctactgaaa acaatgaatc tgtgctatta ttgagaaaag ttccattttt
    15721 gagaaggatg gtttgaatga aagaaatcag acgaaaacat ttccgttttc ctgcgtacga
    15781 tgatgagtta ggcgtaaaat taaccaacga caagaagcga gtattatttg acggacaaga
    15841 agatcttttg actgaacaga atcagtcccc gtttgaacag atgaaagaat ttcatttcac
    15901 gaacacgaac accgagaaaa tccgtcgcaa gcgtcgtgca acaaatggat tgaagtcagc
    15961 tccgcgtaag gaaaatttgg aaaagcataa aggaaaatta ccggattata ccggccatta
    16021 ccaatcagaa atcacatcga caggacgaaa aaaacttttt ggtgattcct ctcgttctgt
    16081 cagacaaagt gaaacaagga cagagtccaa gcatactaca cgaaacgcat ctgttgacac
    16141 gaaggtgcgg gagcgttctt attttgttcc taaatatgta cccgcatcga ttatcccaga
    16201 ccctagagaa agcaaaattt ctgaggatca tttaatcgaa tcgatgaaaa aaacggatga
    16261 ctcttatctg ttgttcgata cagaaccgac gccgtatcaa gaaaaaagaa atggtgagcc
    16321 gtctgtacga acattcaatc gaacaaaaac gttagaggaa acaaagcagt cagaagaaaa
    16381 aaaatcgaaa aaaagccatg gtatcttaga acgctcatta caaggattga tcgatgatca
    16441 aggaaactct ttgggagaaa atagctattt taaataagtg atatacaaat ttatattcgg
    16501 ctacagttgg aaaggaaacg ctgcggcgtt tccttatttt atactctatc cgaaagttta
    16561 tatcgtatcc tcaaattttt ttgagtttaa gcgaattttt tctcccaata agtaagaaaa
    16621 taatggcaaa ataaaaaaa
//
LOCUS       DACDEG010000051        16596 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.053, whole genome shotgun sequence.
ACCESSION   DACDEG010000051 DACDEG010000000
VERSION     DACDEG010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.053"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(251..616)
                     /locus_tag="IV142_001720"
     CDS             complement(251..616)
                     /locus_tag="IV142_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485380.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            824..1306
                     /locus_tag="IV142_001721"
     CDS             824..1306
                     /locus_tag="IV142_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485381.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1503..2393
                     /gene="pstS"
                     /locus_tag="IV142_001722"
     CDS             1503..2393
                     /gene="pstS"
                     /locus_tag="IV142_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAP8485382.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            2718..5189
                     /locus_tag="IV142_001723"
     CDS             2718..5189
                     /locus_tag="IV142_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8485383.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(5292..7028)
                     /locus_tag="IV142_001724"
     CDS             complement(5292..7028)
                     /locus_tag="IV142_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP8485384.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7025..7729)
                     /locus_tag="IV142_001725"
     CDS             complement(7025..7729)
                     /locus_tag="IV142_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8485385.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7860..8363
                     /locus_tag="IV142_001726"
     CDS             7860..8363
                     /locus_tag="IV142_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP8485386.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8323..8661
                     /locus_tag="IV142_001727"
     CDS             8323..8661
                     /locus_tag="IV142_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP8485387.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            8682..10100
                     /gene="ffh"
                     /locus_tag="IV142_001728"
     CDS             8682..10100
                     /gene="ffh"
                     /locus_tag="IV142_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP8485388.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10212..10790
                     /locus_tag="IV142_001729"
     CDS             10212..10790
                     /locus_tag="IV142_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP8485389.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10794..11315
                     /locus_tag="IV142_001730"
     CDS             10794..11315
                     /locus_tag="IV142_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8485390.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11394..11948)
                     /locus_tag="IV142_001731"
     CDS             complement(11394..11948)
                     /locus_tag="IV142_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8485391.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12201..12476
                     /gene="rpsP"
                     /locus_tag="IV142_001732"
     CDS             12201..12476
                     /gene="rpsP"
                     /locus_tag="IV142_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP8485392.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12488..12739
                     /locus_tag="IV142_001733"
     CDS             12488..12739
                     /locus_tag="IV142_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP8485393.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            12864..13655
                     /locus_tag="IV142_001734"
     CDS             12864..13655
                     /locus_tag="IV142_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485394.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            13825..14346
                     /gene="rimM"
                     /locus_tag="IV142_001735"
     CDS             13825..14346
                     /gene="rimM"
                     /locus_tag="IV142_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP8485395.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14336..15097
                     /gene="trmD"
                     /locus_tag="IV142_001736"
     CDS             14336..15097
                     /gene="trmD"
                     /locus_tag="IV142_001736"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP8485396.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15233..15580
                     /gene="rplS"
                     /locus_tag="IV142_001737"
     CDS             15233..15580
                     /gene="rplS"
                     /locus_tag="IV142_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP8485397.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            15838..16131
                     /locus_tag="IV142_001738"
     CDS             15838..16131
                     /locus_tag="IV142_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP8485398.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         5592 a         2934 c         3392 g         4678 t
ORIGIN      
        1 cacaactgtt tttagtgttt ggagagaaga aaagaagtaa aaaatagaaa aactagtgat
       61 agttttggtg atagtgatat taaaaatcag aaaagataga aaaagcagga gatagataaa
      121 atgctgatat ttcagcattt tttaagagta taaaagagaa aaaaagtaat aaatgtatac
      181 atgatataag aaaaacatat atgtataaaa agaagaaagt agcttctctt tgtacatata
      241 tggtttgttt ttatggatgc cgagcttttt tataagataa ggctaccgcc cataacgacc
      301 caaggatttg tggaactaag taaatacctg caagaagcaa gatcagctgg gtcggtttta
      361 aaggatgata gaagtaacgc cattcagtaa aattggcgca aaataaatag gaagtgacag
      421 tagatataat attggcaatg aataaagata taatacgcct gttcctttga aaataatatc
      481 cagcaaaaag agataaaaat atcaatgtga tgaacccgct tacggaatga taacgaaaat
      541 caagcagtaa ggcgaaatgc acataaggaa caaaagataa aagcaacgga aggtttttcc
      601 agcaagtttt tatcatcttt catcaccacc taacaagtgt tattattttg ttagttttat
      661 tgtaaattga atcctgcaat aatcaattac tttttcatta tttctagatt aaatgaataa
      721 actctcatgc cattatctct aaaaaaaaag tttttccccc taattttttt agatagaaaa
      781 ttgtatagta tgatgagaga aaaagtagtt tgggggaacg ttaatgagaa gaaaagcagt
      841 aatcgatcgg ctttatttat ttttttggtt ttttcctatc gttttgctac cttttacatg
      901 ggtaaatgct gttgtacaag gtgcggagat attaggcctg atccaaggaa gcggatttgt
      961 ggtttctagg aattttccag ctttgactgt tttctattta gtgataagtg taggtctctt
     1021 attaccaaaa tctctgatca atcactacaa ttggcagctg ctttactttt cttgcttatt
     1081 agtatttaca tctatgtttt ctttgctgcc tcgcttgatt atcggcccta atggtttgag
     1141 cgagagtgga ttgaaggatt cagcttattc ctccatgtat ttcttgacga tcaaaccatg
     1201 gttctattta gtaattgcct catttattct tgcaacattt ttttacttct ggacagaaat
     1261 caggataaaa agtgaacgat ctgtatctat aaaaaataac tattgatggt ggatcatcag
     1321 taaaaaagca tatgagctgc gtaaaatgca tgtttctcat atgctttttt tattattatg
     1381 taaagaatgc gaaaaacgtt tacatgattt ttacataact taactatatt tttacagcaa
     1441 ccatacgagg gatttacagt cgactgataa actttagttg taaataagaa aggagcaata
     1501 aaatgaaaaa agtattgttt actctaggta tgatgggaat tcttttagcc ggatgcggct
     1561 caggatcagg aaatacagat ggctctgcca cacaaaataa ttcaagtgac tcaaatgaac
     1621 aagtaaaaat cgttgcggta ggttcgacag cattacagcc gttagtcgat gcagcacaag
     1681 aaagctttgt acaagaaaat ccaaattatc aaatctctgt acaaggagga ggaagcggaa
     1741 ctggtttgag ccaagtcgaa gctggagcag ttactatcgg aaactctgat gtatttgcag
     1801 aagaaagaga cggagttgat gcttcaaaat tagtggatca taaagtagca gtggtcggga
     1861 tggcgccaat cgtcaataaa gatacagatg tcaaagatat caccaaacag gaattgattg
     1921 atatctttac aggaaaaatc actaactgga aggaagttgg cgggaaagat caaaaaatca
     1981 acgtcgtgaa ccgtgcaaac ggcagcggta cccgcgcgac attcgaaaaa tggggactgg
     2041 atggtgctac ccctgtgcag tcccaagaac aagattcatc aggaacagtt cggcagctgg
     2101 taagtcaaac accaggagca atcagctatt tagctttctc atatctcgat gattccacac
     2161 aagcactaag tatcgacggt gtagaaccaa aagaagagaa tgtagcagat aacagctggg
     2221 gaatctggtc atatgaacat atgtacacga acggaaaacc ttcccctgaa gtccaaaaat
     2281 tcttagacta catgatgaca gaagaaatcc aagaaggacc tgtcaaagaa ttgggctatt
     2341 taccaatcac aatgatggaa gtagaacgtg atcacgaagg aaatatcaaa taactagcag
     2401 ataaacatgc cacggtagtc gttactatta aggaaaaatc aactatgttt cccacatata
     2461 catttttcct ttaattgtga aaaatagttc ctcatatttt gccgtttttt gagctaatac
     2521 cgagggtcag ctgattaaac accgtttatt cgaaaaaaag agctacgaca tagaactgtc
     2581 gtagctcttt ttttgctata tgacaggaat gaaaggtgtt tgcaagctta atgtgaggaa
     2641 cactttccat atctgttcta tactggatag gagaagtttg gaaatatcca gaaaacgaaa
     2701 tcaatcgagg tagccacatg aagaataaat ggataaatcg aagtaaaaaa aagaaagtaa
     2761 cacaaagatc atcagataaa gtcatccgtc tttttccatc gtataaagaa ggattaacgg
     2821 cagatcaagt caaggaacga atagaaaaag gtgcagcaaa taattcagtc gatcccacat
     2881 tcaaaacaaa tcagcaaatc gtattggaaa acatatttac gtatttcaat ttgatctttc
     2941 ttatcttagc cattttgctt tgtttagtag aatcctataa gaacttgact tttttaccag
     3001 ttatcatagc caatacagga atcgccattt ttcaggaaat tcgatccaaa aagatcttgg
     3061 acgaactaaa tgttttaaat acagcaaaag tcaatgtcat acgggacggg atagaacaaa
     3121 caatagatat agaagacctt gtaatagatg acattgttat tttagagact ggacatcaaa
     3181 ttccagcaga tgcagaggtc gtggaaggaa aactgcaagt taatgaggct ttattgacag
     3241 gcgaagcaga tgaaataacc aaagaaatag gcgatccatt aatgtccggc agtttcattg
     3301 tttccggaaa agcttacgcc cgattagaca aagttggagc agattcgtat atttcccagc
     3361 tgacagtcaa agccaagaca atgggagaag gagaacagtc ggagatgatt gcttctctta
     3421 atcaattgat caaatggatt gggatcatca ttattcctat cggtatcctg cttttttctc
     3481 aaagttactt ttttaatgga aacacgatca aagaaagtgt cgttgcaatg gaagcagcat
     3541 tgatcggtat gatacctgaa ggactgtact tgctgacgac gattgcatta gcattaagtg
     3601 cgactaaact agcaaagcaa agagttcttt tacacaatat gaaaagcatt gagacattgg
     3661 caagggtaaa tgtcctttgt gtagataaaa caggaacgat cacagaaaat aaaatgtctg
     3721 ttcaaaaagt gatcgtctct aaaaaacaag aaataatcag tcagtcagat caattggaag
     3781 catgcattgc tgactatgca aaagcgatgg caggagacaa tgcaacaatg gaagctgtta
     3841 aagcatattt tacaaaaacc actgatcaat cctatcacca tgttattcct ttttcttctg
     3901 tccaaaaatt cagcagtgta gcgttggcag acaaggtgta tgttttagga gcgcccgaaa
     3961 tggttttgcg cgatcaattg ccggaatatg cggaagagtt tgtttctttc gctgaaaaag
     4021 gctatcgcgt gttggtcttt ggtgtgtacg atgggattct tgaagaacca gtattgacag
     4081 aagcagtcac accattaggc tatattctga ttgctaatcc tatcagaaaa gaagcaagag
     4141 ccacatttga ttacttcaaa aaacagcaag tagccatcaa agtgatttct ggagataatc
     4201 cgctcaccgt atccaatgta gctgtccaag cagggatttc acatgctgat cagtacgttg
     4261 atgcatctaa attatcagaa gaagaatatg aagcagctgt cgaaaaatat acagtttttg
     4321 gacgcgtcaa gccagaacaa aagaaaatct tcgttcaatt gataaagcgt aacaatacag
     4381 tagctatgac aggtgatggc gtgaatgata ttctagccat gaaagaagca gactgcagta
     4441 tagcgatggc ttccggaaat gaagccgcca tgcaggcctc acaagtggtc ttacttgatt
     4501 ctgacttttc acgcatgcca gaagttgttg cagaaggacg gcgcgttgtg aacaatattg
     4561 agcgatcagc cagcttgttt ttagtgaaga atattttctc ctttttgctc tcactgtttt
     4621 cagtgatctt tgctctgacc tatcctttag aaccatcaca aatcacattg attagtttgt
     4681 ttactatcgg acttccatct ttcttgcttg ctctagaaga aaacaaaaag cgaatcagag
     4741 ggaaattcat catgaatgtg atggaaaaag ctgtgccagg tggactaaca gatatgatcg
     4801 tagtcggtgc gttggtgatc tgcggggtga ctttgaatct aaataaaaca gatgtttcta
     4861 cagcatctac catgcttttg attgcagtag ggttcctcgt tctctataaa atctgttcac
     4921 cgctgaataa attcagaagt cagatcattc ttttctgtgc tagcggtaac tttttttcgg
     4981 tcatcttttt gcataagctg ttttcaatca ccggtatctc agctgtatca cttttgctgt
     5041 tgagcattct atttttctcg gcagattcga tttttaggca tttgaccaca ttggtagaat
     5101 atttgtttga acgtaaaaat gaaaaagagc aaaagccgtt tagtcgcgta gctaaaaaat
     5161 ttctatcgat atttaaacga gaaaattgac gaaaagtatc tcaaaaaaga gtaaaaaaga
     5221 acaaccactc gtgtatcttt cgaagcagga tgagtcatcc aactttaaaa cgcacgagca
     5281 gttgctcttt tttacatttt cggaatggta acggtgaaag tcgaacctgt tccaagatga
     5341 ctgtctacag taatcgtacc attaaggagt tgcacgtagt cttttacaat cgctaaccct
     5401 aatccggtcc cgcctgaatg tcggcttcgc gccttgtcta cgcggtaaaa acgctcaaag
     5461 atccgttcct gatcctcaat gtcgatacct ataccaaagt cttggacaga aaacgataga
     5521 tcatctgttg cctgatagcg gataataatc tcgctgtcgc tttttgaata ttggacagca
     5581 ttttcaatca gatttttgat aattggataa aggatatctg ttttagtagt aaatggttcg
     5641 ttctttccaa tcaatcgaat cgtcaaccgt ttcttttcga tgattggctg ataattttga
     5701 acgatttgct ggaaataagg ttctgtataa attgtctgga tctcgtaaga attctcgcca
     5761 tcttttgaca gctggatgat ttcccgaatc agtttatcta accggatcgc atctttttgc
     5821 atgatctcaa gaaaagaagt cagagtttgc gggtcatctt ttgcaccatc cagtaaagtc
     5881 tcggtaaaac caatcagtga agtcacagga gtttttaatt cgtgagagac gttgccaacg
     5941 aaatcctttt gtagcttttc taaacgtcgc actttcgtta agtcatacgc cacccctaga
     6001 atctgtccgg taccttctga attagtaaag tagcgcaaac tcaagtctaa tgtctgccga
     6061 ccttctgtaa gggtgatctc ctgatggatc agcggatgct caggagtcac ttgatgaatc
     6121 agctgaatga gttttggctc ttgaatgact tctgtgtacc gctgttcagg ttgatagctt
     6181 accacgttta aatgtagctg catctttgga ttaagcagta gtaaacgaga atcagcatca
     6241 tcaatcagga atacgccaat catcagttcg ttcagtaacg tatataattg ctcttctgtg
     6301 gtagtacacg cccgatacat cttactcatt tgttcgctca acgcattaat cgtctgatac
     6361 aattcttccc attgatcggg tgtttgcatg attacttccg cctgattagg attttgcacc
     6421 atttttttta gtacgggcag cacggttttc aatggctggt ttttttgata gatcagataa
     6481 tagatcattc ctgcaagaac aaggaagaaa gacagaaaga agaaaaagac ccaacggcga
     6541 aaactctcag ttctcggtaa aaaaccgctt gtcggttctg cgatacgtaa aatggcttct
     6601 aactggccac tttttttgac tggtaatgcg acatataaaa gatcctcatt caaagtagta
     6661 cttttgcgca aagcgctacc taggttccca ccattcaata cggctttgat ttcgggacgg
     6721 gagtttcgct gttcattcaa tgtttcattg tttgtgtcaa ataagatctt tccagtagca
     6781 tccattaaag taatccgttc gttcgactga tggacgaatt cttccaaagc agttttgttt
     6841 tgcgctgctt ggaggttttc gacatctaat tgccggatca gcagttgtgc tttttttgtc
     6901 aggtagcttt cttgctgttc cacgatctgc tgttgaaaat aatgggaaat caattgccag
     6961 ccaccaaaaa acaaactaac taaaagaata agaaagaaga gacgttcata aatttttttc
     7021 catttcatcg tttgggttcc tgaaacttat agccaaatcc tctaatagtc accaagtact
     7081 ttgggtgttt tggatctcgc tcgatttttt ctcgcaaatg actaacgtgt acatctacga
     7141 ttcggctttg tcctgcgaag tcaaaattcc aaatgcggtc tagcaatgtg tctcggtcga
     7201 tcacacggtc ttttcgtttc atgaaataaa caagtaattc aaattctttc ggtgttagtt
     7261 caatcagatg gtcgtctacg gtcacttgat aattggtcaa atcagcagtg atttgtccaa
     7321 tcgatagata ttcaggctca gcttcatctt ttgattgctt acgaggttca attcgtctga
     7381 atattgcttt catacgggct aatacttcgc gaggactaaa tggtttggtc aaatagtcgt
     7441 cagcacctat ttctaggcca atgatccgat ccacttggtc atctttagcc gtcagcatca
     7501 aaatcggtgt ttcatttttt tcttgacgca gccgctgagt gatctccatg ccatccatcg
     7561 ttggaagcat cacatctaaa atgatgaaat caaatgattg ttctaatgct aattctaatc
     7621 cttcgcctcc atctgtggca ctggttacct tgtagccttc tttttccaaa ttgaacgtca
     7681 agagcgttac aatagaaggt tcatcatcaa caactagtat ttttttcatt caaatctcct
     7741 ttaaataggt ttcattctgt ttatatttta gcatagaatc agtacaaagg gctattttac
     7801 aaaagaaata aatggtataa taggaaggca ctttgctgaa tagaaaggaa gagcagtcaa
     7861 tgattggaat cagtgcatgt ttaggcggtc tggcctgtcg ctatgatgga cagaaaaaag
     7921 aagtaccaga attgatggaa ctagttgaaa ctggagaagc tgtcatggtc tgtccagaag
     7981 tgatcggcgg attaccaatt cctagagatc ccgctgaaat cattggtgga aatggattcg
     8041 atgtgtggaa agatcaagca aaagtatgga cgacaagcgg cgaagatgta acggaagcgt
     8101 acaaaaatgg cgcgatcaaa gcctatcaaa aattgcagga aaaacacatt gatctagtca
     8161 tcatgaagga aaaaagccct tcatgcggaa cccattcgat ttatgacgga agtttttccg
     8221 gaataaaaaa aaatggtgcc ggagtggcta ctgcttattt tattcagcaa aaaatgactg
     8281 tgctctctga tgaagaatgg ctgaaaacta gaggtgagca gaatggaaat cgaaaagacc
     8341 aatcgaatga acgcgctctt tgaattttat tcgaccttat tgacagaaaa acaaatgaac
     8401 tacatggaat tatattatgc agatgatttt tcattaggtg agatcgcgga agaatacgaa
     8461 gtaagccgac aagcagtata tgacaatatc aagcggacaa gcaagatctt ggaggattat
     8521 gaaaaaaaac tccatctttt ttctaattat attgttcgag aacaggtatt ggaaaatatg
     8581 actgcatata ttacagataa gtacccggaa gataaaaaac tacttgaata tgtacagcaa
     8641 attcaagcaa tcgaagaata aataaaagga atgaagaaaa tatggcattt gaaagcttaa
     8701 ctgaacgtct ccagcaggcg atgggaaaaa taagaaaaaa aggaaaagta tcagaagctg
     8761 acgtaaaaga aatgatgcgg gaaatccgcc ttgccttact agaagccgat gttaacttgc
     8821 aagtagtcaa agattttact aagcgcgtac gcgaacgtgc ggtaggggca gaagtgctgg
     8881 aaagtttatc acctgcacaa caaatcgtaa aaatcgttga tgaagaattg acgatcacac
     8941 ttggttctga aacagcagag ttaaataaat caccaaaaat accaactgtt atcatgatgg
     9001 ctggtcttca aggggcaggt aaaacgacct ttactggtaa attagccaat tatttaaaga
     9061 aaaatgaaaa tgctcgtccg ctcctaatcg caggggacgt atatcgacca gctgcgatcg
     9121 accaattgaa agtattaggt caacagctag acgttcctgt atttgatatg ggaactgaag
     9181 tcagcccggt agagatcgtc cgtcaaggga tggagttagc aaaagaaaag aaaaacgact
     9241 atgtattgat cgatacggca ggacgtctgc atatcgatga aacattgatg gatgagttga
     9301 aacaaatcaa agaattgacg caaccaaatg agattttgct tgttgtcgat gccatgactg
     9361 gtcaagatgc cgtcaacgta gctgatagct tcaaccagca attaggtatt acaggggtcg
     9421 tcatcactaa acttgatggg gatactcgag gcggggcagc actatcgatc cgctcagtga
     9481 caggcgcacc aatcaaattc atcggttctg gtgaaaaatt gactgactta gaagtattcc
     9541 acccagatcg tatggctagc cgtatccttg gtatggggga tatgctgaca ttgattgaaa
     9601 aagcgcagca agactacgac gagaaaaaag cggaagaact tgcacagaaa atgcgggaaa
     9661 attcattcga tttcaatgac tttattgaac agcttgatca agtgatgggt atgggcccac
     9721 tggaagatct aatcaaaatg atcccaggaa tgaatcaagt ccctggaatc gaaaacgtca
     9781 aggtagatcc aaaagatgtt gaacgcaaaa aagcgatggt ttattctatg acgccagcag
     9841 aaagagcaaa tcctgatctg ttgaacccaa gccgtcgtcg ccgaattgct gccggttctg
     9901 gaaacagtgt tgttgaagtg aatcgaatga tcaaacaatt caaggaatca agaaaaatga
     9961 tgcagcaaat gtccaaaggg gatatgaaca tcccaggtat ggaccaaatg tttggttcag
    10021 gcgtcaaagg aaaactaggc aaaatggcga tgaatcgaat gatcaagaaa aataagaaga
    10081 aaaagaaaaa acggaaataa gatgaaaaaa acagcttcgg tacagactga aaatgtctac
    10141 tgccggagct tttctatcag caatttttat tctttactaa tacaattact ctaaacgaag
    10201 aggtggcaca gatgagatgt ccgtggagtg aacaaacaga aagtatgcgg caatatcatg
    10261 atactgtctg gggtgttcct gaatatgatg accaaaagct gttccgaaaa ttgatgcttg
    10321 atattaatca agccggattg agctggcaga cgattttaaa taaaagtgaa gcattcgatg
    10381 aagcatatga tcgattcgat atcaaaaaag tcgcaaatta tggagaaaca aaaatcagtg
    10441 aactaatgga aaacaaagga attattcgta accgaagaaa aatcgaagca gccgtccaaa
    10501 acgccaaagc agtcttgaaa attcaagaag aaatcggcag tttttcagag tatctttggt
    10561 cgttttctaa tggaaaaatc gttgattctt cttatcattc acaagaagaa gtcccaacaa
    10621 caagtcctct ttcagatgaa ttagcaaaag atctaaagaa aagaggattt aaatttatcg
    10681 gaagcacaac aatctatgct tttttagaag ctgtcgggat catcaatgat caccttgaca
    10741 gttgtttccg caaagaagag gtcgtacgta tcggcagaaa gggcgaataa agcatgcgaa
    10801 aaatcatatt ttatggtgct ataagtttag atggctattt ggcaacgaaa acggatgatc
    10861 ttcaatggct ttttgacact ccaacaggta agcaaactac ttatgaagct ttttatgaaa
    10921 caatcgatac gaccatcatg ggcagaaaaa cttatcagga agcaaaaaaa tatttggaga
    10981 ccgaagcaat ttatccagag aaaaagaatt tcgttttttc cacccacgca caattacaat
    11041 tgccagatgc tgaggtcgta aacgaagatc ctgttgcttt tctaaaaaat ctacaaatga
    11101 cagaaggaaa agatatctgg gttgtgggtg gcggtcgatt gatcaaaccg ctattggaac
    11161 atcaaatgat tgatgaatgg tatatccaga ttacccctgt attattaggg gacgggattc
    11221 ctttattcca aaaaggaaca tatgaagcgc gttttgaaat gctagacacg acacgattcg
    11281 gtgaatttat cgaattgcat tatgtacgaa agtagaagta aataaaataa aaaaggcaac
    11341 tatatgcaga aatgtcctgt acatagttgc cttttttatt accgttatgc tttttactct
    11401 gttaatttgt tagaaaaatc tagatacgca tctaaaatcg tttggaagaa acttaatgtt
    11461 ccttcaacga tattaccatt ttcatcaatt agattcgcaa cgtttccgat atatgcttcc
    11521 ggttgctgca aggttggcac attcaaaaag acaagtgatt ggcgtagatg gtggtttgcg
    11581 ccaaagccgc cgatcccacc tggtgatgca gtaacgacta aacctggttt atgatcccaa
    11641 acactgtgtc cataaggtct tgagcctaca tctaaagcat ttttcaatac tgctggcaca
    11701 gaacggttgt attcaggagt aacgaaaacg acaccgtcta gtcccctcac ttcctcacga
    11761 aaacgagtcc attcagcagg agcctgttct ggagtttcga gatcctcatt gtaaaatggc
    11821 agatcatcga tttttacaaa tacactttcg tacccttcag gaagcaattt cgcaaaagct
    11881 tctgctactt ttttgttgta agaatctttt cttaaactac caataaaaaa tccaattttt
    11941 tttgtcatac gatttttcct ccttcaaact atctataata atcttacaaa agataagtaa
    12001 ctggagcaaa taaaaacact tactgacaaa agctacatag aagatagatt acttattttt
    12061 aacaaaaaag aagtctaaaa tcttctattt tctaaaaaaa atcgaattta atcaagctgt
    12121 aaagaaaaaa agctttacag ggtaaaaaaa aactgttaca ctacatcttg tgaataaaac
    12181 aatggaggtg gaaataaata atggcagtta aaatccgttt aaaacgtatg ggttctaaaa
    12241 agagtccgtt ttaccgtatc gtcgtagctg attcacgttc tcctcgtgat ggacgtttca
    12301 tcgaaactgt aggtacttac aatcctttga aagaccctgc agaagtagtt ttaaaagaag
    12361 acttagttct tgactggtta tcaaaaggtg cacaaccttc tgacactgta cgtaacatcc
    12421 tttcaaaaga aggcgttatg caaaaacacc acgaagctaa attctcaaag aaataaggtg
    12481 accggatatg aaagatttaa gcgacttagt cttaacaatc gttcgtccgc tagtcactta
    12541 tcctgatcaa gtcaagttag acgtcgtaga gtcgaaagac ttttacgagt acaatctaac
    12601 agtggctcct gaagatattg gccgtatcat tggaaaacaa gggcgtgttg ctaaagcaat
    12661 ccgcacaatc gtttacggtg ttcggatcaa tgcaccgaaa aaagtgcgtt tgaatatcat
    12721 cgatgataag gaaaagtaaa taaacgtgct gtactcgaat tttcgagtgc agcttttttg
    12781 ttttttttat taattgaaga aatgattatt tggcaaaaca agcaagaaag cgtatcctta
    12841 taggaggagg gagtgaacag ccaatgagaa agaaaatgtt atttattcca tttgctctat
    12901 tgttaagcgc ttgtagttcg gttactcaag aatcagacaa tacacatcta tcaagcagta
    12961 tatctgaaac tgttaccagt ccagaaaaaa gtagtaccaa aacggcttcc accactgaac
    13021 aaacaatggc tacctcaaat aatgagaaaa agactgcttt agaccagttg aaagaacaac
    13081 agccaaatgt tcccatgcct ctagatgtac cagtctcttc aggctatctc aatatcgcag
    13141 cgacacatac aaagcaaggt tattctattt tatattacag aacagatcgt cccctcgggt
    13201 tgaatgctga cgaattgaat caagaaacgc ctattgcaac ctatctatat caatatgggt
    13261 ttgcttctag tcaagaaaca atccaagttt tgcagccatt tgagatcgac acaaatggtc
    13321 agcaagtgga cttaggttct cgaatcacag ggtatcagca aggtgcagca ggttcaagtt
    13381 ttcttgaatg gcaagaagga aactggcgta ttcgaatccg tgggaataat atagaaggcc
    13441 aagatccctt actattagct aaagaaatcg tagcttattt ggaagaaaac agtctaccag
    13501 cacctgaaca atttggtaaa attactgttg atatgggtga tacgacgaat cgagccgtag
    13561 aagtaagctg gcaagaaccg aaaaatgcct atacgatcac tcatcaagat cctatgtctg
    13621 cgttgaagat ggcagtttct atgaagaggt tgtgaaatca gcgcgctgat ctgagcccat
    13681 catttaactc caagtcacag gaagtaatca aaaatagcac gccgcatttt tgcataattt
    13741 tggcttaata ccttaaaaga aaactagaat tgcctcttca cacatgctaa aataaaacag
    13801 acaaaaaaga aatggggata taagttgact gaatacttga atgtaggaaa aatcgtaaac
    13861 acacaaggta tcaaaggcga agtccgtgtt atctcaacaa cggatttccc agaagaacgt
    13921 tacaaaaaag gaacggtgct aacactattc caagacggaa aagcacctgt ggaattaaca
    13981 gtcaaaagtc atcgcaaaca taaaaatttt gacttattga gttttgaagg acatccttct
    14041 atcaatgatg tggaaaaata tcgggatggc atattaaaag tgtcaaaaga caactctgta
    14101 gatctagctg aaaatgaatt ctattaccat gagatcatcg gtctgcatgt attagaagat
    14161 ggaactgagt taggtaaagt aaaagaaatc ttgtcaccag gtgccaatga tgtttgggtc
    14221 gttcaacgtc cgaaaaaagc tgatatattg cttccatata tcgcttctgt cgtcaaagaa
    14281 gtcgatttag aagctggagt ggtccgtgta gaaattccag aaggactgat tgacgatgaa
    14341 gattgatgta ttgactttat ttccaagaat gtttgaaggt cctatggggg aatccatcat
    14401 cggtaaggct gtagataaag gattattaga tatcaatatc tctaacttcc gcgattattc
    14461 agataacaaa catcagacag tagatgatta tccatacggc ggtggagcag gtatgctgct
    14521 caaagtccag cccatctatg ataacatcca agccattgaa aaagctgcac cggatgtcaa
    14581 aaaaagagta attcttcttg acccagctgg taaacgattc gatcagaaaa tggcagaaga
    14641 attttcacaa gaagaacact tagtattcat ttgtggtcat tatgagggat atgatgaacg
    14701 tatccgcaca ttagtgacag atgaagtttc cttaggagat tatgtcctta ctgggggaga
    14761 attaggtgcg atggtcatga tcgatgcaac agttcggctt ttgccagatg tgttagggaa
    14821 tcagacttca gctcaaacgg attcttattc aaccggttta ttggaacatc cccaatatac
    14881 tcgcccggca gaatataaag ggatgaaagt accagaagta ttgacaaatg gaaatcataa
    14941 attgattgaa gaatggcaat taaaagaatc actaagaagg acctatcaac gtcggcctga
    15001 tcttttggaa acacttgaat ggacacctca aatgacgaaa tttttagaag aaatcaagaa
    15061 agaagaacaa gaaaaaacag cagatgaagc caagtgactt tttctaaaat cttctttaca
    15121 gtaggttgat aatatgttag tattttatgg tgagtgcaaa agcacttaac tattacgata
    15181 ttccgctgtg agaaaagctc ataagaatat ttggaataag gagaagaaaa ccatgaatcc
    15241 attaatcgaa gaattaacaa aagaacaatt acgttctgac atcccagcgt tccgccctgg
    15301 tgacactgtg cgtgttcacg caaaagttgt tgaaggtact cgtgaacgta tccagttatt
    15361 tgaaggtgtt gttatcaaac gccgcggtgc tggaatcagc gaaacttata cagtacgtaa
    15421 agtttctaac ggagtgggcg ttgaacgtac attcccattg catacaccac gtgttgcaaa
    15481 aatcgaagta gttcgctacg gtaaagtacg tcgtgcaaaa ttgtactacc tacgtgcatt
    15541 acacggaaaa gctgctcgta tcaaagaaat ccgtcgttaa tcattgttat cagactgaga
    15601 aagaaccctt ggtattcaag ggttcttttt tcttagcatg taatttgaac accctgatcc
    15661 agttttttct ttaattattg gtaggggaga ataatggatt agaataaatc tacctataag
    15721 tagtagtaaa aaaaagagca ctatgataat atctttctta agagtaaccg ctttaataaa
    15781 aaatccagaa tattctaaca taaatggagt attcttagaa tcaaaaggag aaagaagatg
    15841 gtttatactt atacggagca agaaaagatg aaagttttag acacattcat gaatgatgga
    15901 aaagtaaaac aagtaccaag ttcaacaaaa aagaaataca tcctattgaa agaagtcttg
    15961 aaacgctttg accatggagt gacttataca gaaacagagg ttaattcgat actgctgaac
    16021 gtattttcta gtggcgatta tgtcgaacaa agacgttatc taatcacatt tggctttttc
    16081 aaaagaagtt cagacggtcg ggcatatcag atgatgggaa ttgaaaatta gaagttttaa
    16141 tttatactca ttgaaaaaag ttcaagtatg cgatattggt tctacaccgc atacttgaac
    16201 tttttttgtc atctgtatct ataatacaaa gccaagaacg ttattcaaaa tatggatagc
    16261 tatgataaaa gctaatgagc gattcgcagt taaataagca atcgtcagta aaatcgttcc
    16321 aggagcgtac atgaaaaaat caattggatg cgttacatga ataaagataa ttctctttta
    16381 tttgtacgaa taaataccaa aacgtacctc ctaaaattag attttggaca gatgcccttt
    16441 aaaaagataa caagatttcg ttcttgaaac aatatatatg cttttattcc aatgaggttg
    16501 tgaaaacagt attttgtcct gagcgttaga aaattgaaaa tggggaatag caactcgtac
    16561 tttaccattt tttaagtgaa tacaagatca gctaat
//
LOCUS       DACDEG010000052        16431 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.054, whole genome shotgun sequence.
ACCESSION   DACDEG010000052 DACDEG010000000
VERSION     DACDEG010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.054"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..546
                     /locus_tag="IV142_001739"
     CDS             <1..546
                     /locus_tag="IV142_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAP8485399.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSLVQFRKTTVTVV"
     gene            735..1403
                     /locus_tag="IV142_001740"
     CDS             735..1403
                     /locus_tag="IV142_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP8485400.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1603..1833)
                     /locus_tag="IV142_001741"
     CDS             complement(1603..1833)
                     /locus_tag="IV142_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485401.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1960..3342)
                     /locus_tag="IV142_001742"
     CDS             complement(1960..3342)
                     /locus_tag="IV142_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP8485402.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(3498..4685)
                     /locus_tag="IV142_001743"
     CDS             complement(3498..4685)
                     /locus_tag="IV142_001743"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP8485403.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4692..5723)
                     /locus_tag="IV142_001744"
     CDS             complement(4692..5723)
                     /locus_tag="IV142_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8485404.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5739..7193)
                     /locus_tag="IV142_001745"
     CDS             complement(5739..7193)
                     /locus_tag="IV142_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8485405.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            7368..8273
                     /locus_tag="IV142_001746"
     CDS             7368..8273
                     /locus_tag="IV142_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8485406.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            8364..9041
                     /locus_tag="IV142_001747"
     CDS             8364..9041
                     /locus_tag="IV142_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8485407.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            9322..10218
                     /locus_tag="IV142_001748"
     CDS             9322..10218
                     /locus_tag="IV142_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8485408.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(10273..11643)
                     /locus_tag="IV142_001749"
     CDS             complement(10273..11643)
                     /locus_tag="IV142_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8485409.1"
                     /translation="MEEKKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11881..12627
                     /locus_tag="IV142_001750"
     CDS             11881..12627
                     /locus_tag="IV142_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8485410.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12624..13463
                     /locus_tag="IV142_001751"
     CDS             12624..13463
                     /locus_tag="IV142_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8485411.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            13464..14414
                     /locus_tag="IV142_001752"
     CDS             13464..14414
                     /locus_tag="IV142_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP8485412.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14555..15307
                     /locus_tag="IV142_001753"
     CDS             14555..15307
                     /locus_tag="IV142_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485413.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            15326..>16431
                     /gene="queG"
                     /locus_tag="IV142_001754"
     CDS             15326..>16431
                     /gene="queG"
                     /locus_tag="IV142_001754"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP8485414.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVI"
BASE COUNT         5335 a         3082 c         3306 g         4708 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg gcgtatcaaa tgaaagctta tagaaacaaa
      301 ttgaaggagt acaaaatctt tcaaagcatg tctcggaaag ggaactgctt agataattca
      361 cttatggaga atttttttgg cttattaaaa caagagattt ttcacgggaa agtttataac
      421 tgtttcgagg aattaaaatc agccatcgat agctatatct attactacaa taatgaacgg
      481 ataaaacaga aattaaactg gcaaagtctc gtccaatttc gtaaaacaac cgtaacggtt
      541 gtgtaaaaat atcattttag agcataaaaa aaaggagtgg atttctccac tccaaaaaag
      601 tctaactttt gggggtcact acagtgtcac agtccttttt ttagtaaaag gatacatttc
      661 ttccttgtat attccatgcg tgttcgtgag aaagcatata taatttaatt aacagatagt
      721 tagggggaga aaaaatgatt ttttcaatta caatgaaact ggtgttcggg ttgatcggtt
      781 tattattagt ggtacgtttg cttgggaaga aatccatgtc agaaatcaca ccgtttgatt
      841 tagtctatac gcttgtttta ggtgggatct tagaagaatc aacctatgat gacaatgtcc
      901 atgtagggca tgtcctattt gccattgcgc tttgggcagt aatgatttat ggtatcgaac
      961 gtatcgtcca aaaaaatgaa aaggtcaatc gttgggtcaa aggcgagcca tctgttctaa
     1021 tcaaagacgg cgtcatcaac atgacagagt tgacaaataa ccatatcgag atggagcagt
     1081 tgcgagcaat cttaagacag caagaatgct tttctcttga aaatgccaaa catgttatcc
     1141 tagagaatgc cggtcaaatg agtgtattga aaaaatcaga tgaagacaaa gctttatcga
     1201 tcctgcttgt agatgaaggg caaattcagc ataaagtgct tcagtccaat caattgactg
     1261 aagcatggct tatggagaac ttgaagaaag aaggctatgc agatgtaaaa cagctgattt
     1321 atgttgaatg gtcagaagaa aaagggctct acatactaaa taaagaagac gtcatgcatg
     1381 aatcttaccg aatagatgga tagatagaca gaggttgtga aatcagcgcc ttggcttgtt
     1441 actcaaaact aagcgacttt ttcacaacct ctgatatcta atatacgttg ttccagaagt
     1501 tgcttcttcg aaaaataagc caaaattcca caaaaattta agaagcaatt ttcgaaaaat
     1561 cctttcttat tttttgaagt tgatcacttc tgtcacaacc tctcatgtca caaaccattt
     1621 ttttgtttcc tttaactcaa tataccgtac gtcttcccac aatatcggct gatggtcaat
     1681 cataaagtaa tcttcatagg cttcaccaat gaagtgccct tcgacctcat ccaagagtct
     1741 tccaaattca tcttttaaat tgagctgaat gattactgct ttacgagata aaaaggcttc
     1801 tgtcagtata ctatcgattt cttgcaatgt catttgttgt ttaggaagag gctgtttgcg
     1861 tgcttcttct tcattttttt gataccgttg actagttctt ccatcgcaaa agcagtcccc
     1921 cattttaatc caaatggccg atctcgatac tcgttatagt caatgaattt ttttgctgtc
     1981 cgtctcatct tttccacctt ctaatccact cataccacct gcatgcccgc caacgagaga
     2041 actacgtgcg atggcgcgtc ctccttgcaa gagactatgc gcatggatga ttgattgaaa
     2101 gccgtatttt tttctaatcg tatcgacaac aaaatcaact ttcgcttcat ttatctgctt
     2161 ttcaggatct tcaaaaaggt taagctgtaa acctgtcgcg tatacgaggt cggaacagtt
     2221 cacgcctaca cgccgaatat cctggtagtg gtaatgctgg tggaaaatgg tcaataaagc
     2281 atctgctaga tctctgctag catttgtcgc ttcgattttc atttgctggt gaaacccggt
     2341 cttgccattt ggatcaagat agcctaaaga gtatcctacc cataagctga ctacttgtgt
     2401 ttttgctgca gatcgtctca agcgagtgcc gacttggtcg gccatttctt tgataacgat
     2461 ctcgatttct tctcgtcttg tataatctct atctaggatt tgactatttc cgatactttt
     2521 tgattttggt gtatattctt gtcccaaaaa acttcgatcg atgccccaag catgggcata
     2581 aagctgagta ccgatgaccc ctagcttgct tttcaagtga taataatccg cgtgagctaa
     2641 atcataaaca gaatgtatac ccatttgttt cagacgtttt gctgttcgtt tgccgattcc
     2701 ccaaaagtca gtgatatctg agattttcca taacttttct ggtacatctt cataacgcca
     2761 ttcagcaaac atttgctttc cgtcttttga gccattgtct aaggctagtt tagcaagtag
     2821 cgggttatcg ccaattccta tagtcgtgta gattccggtc tgctcaaaca catctttatg
     2881 gatcagttta gccatgtcgt acgcatttgt tttaccaaag agtgccatgc tatttgttac
     2941 atcaaggaag ctttcatcga cggaatagac atgatggttc gcttcgtctg catatttttt
     3001 atagatagaa ttgatttctc tgttcttttc catataatag gccatccttg gggcaacaat
     3061 atataaatca cttggatagg ggaagggaag atctctggca cggctgatat tggtgatgcc
     3121 gtaagctttt tttgctgcag gactggaagc caagattaat ccgctacccc gtgtttctgt
     3181 cgaatcggac ggataggaca tgactactaa tttagttgta aggggatcaa gatccttttc
     3241 aacgcactct acggaggcgt aaaaagattt gcagtcaata cataaaatat ctcttgaagg
     3301 ttctttttca taaacaaata ccggattttg attcgcccac ataacaatca cctcttcaac
     3361 acaattagaa cacacgttct ctttccgtgt caataaaaaa cagcaatttt caaatacgag
     3421 caaaaggctc acatttggaa attgctgaga ggttattttt atctatttgt ttttagctga
     3481 gatcgttgat ttttttatca aacatgaaca gcttctggat cgatctttgt tgtcccattt
     3541 ccaatgtgtg caacataaaa ttccggagca tagccgacaa ctttcatgta ttcatcatag
     3601 acattgtttt tgaaggcagg tatattttct tctttgacaa gagcaatcgc acagccacca
     3661 aatccagctc ctgtcattcg tgcacctaag acaccttctt gtttttgagc agcatctact
     3721 agtgtatcta attcgatccc agttacttca taatcatagc gaagagagtg gtgagaatca
     3781 tttaacagtt gaccaaatgc ttctaagttc ccagcttcta attcagcttt tgccttcaaa
     3841 gtccgctgat tttcagaaac ggcatggcga gctctgcgta tcaatgtatc atcgccgatt
     3901 tggtcaatag ttgattcaaa ttcttcttca tctaagtctc ctaaagaaga gataggcaaa
     3961 gttttttgca aacgtgccaa ggcttcttcg cattcgctac ggcgttcatt gtattttgaa
     4021 tctgccaatt cccgccgctt attcgtattc atgatgacga ttgcataacc atctagtttc
     4081 actggaacca tttcgtattt cagcgtattt gtatctaata agattgcatg atctagctca
     4141 ccgaagccga tggcaaactg atccatgata cctgaattga caccgatgaa ttcattttct
     4201 acttttttac ctgtttggac tagttccaag cgatctgttt ttaaatcaaa cagatcttct
     4261 aatacaacac ctactaataa ttctaatgaa gcgctactgg aaagtcctgc accattaggg
     4321 attgttcctt ccactaacaa ctcaaaccca tgatcgattg agtgtccagc ttcttttaat
     4381 ttaaggacca ttccttttac gtaatttgcc cagtttgctt ttttatcata ttctagctca
     4441 tctaaagtaa aactaatgac accgatatcg tcaaagtttg tggaataaac taatatttta
     4501 ttatcgtctc gaggtgctgc tacaccttgc gttccataag tgatggaagc ggggaagaca
     4561 tggcctccat tgtaatctgt gtgttcacca atcaaattga tgcgtcctgg tgagaagtat
     4621 tgtgtagttt ttttaggtcc gaatgtttcg gcaaattttg tgtttaagct tcttttgatt
     4681 tccatgatga gttattcctt tcttgttttg attttgaatt ctgtcactgt ttcaaatact
     4741 gatccggctt ttaaatgaat cgaaccaaat gctgagaagc gctctgcacc tggagctgtt
     4801 tgagtctcaa atgtgatgcc accgtgatgt gccagttttc ggttgcgcat ttctggtgtt
     4861 tctgtgccga aatttgcagt gaaaataacc acactagaag catctgtagc aatacctact
     4921 tggattcttt tatcggggct agtcaagcga gcggcttctt tacccaagcc ggtttctttt
     4981 aagaaaaatg gatgatcgat tccgtcaaca agattttttt gatccaaatc actagcaaaa
     5041 acggacgcta attttttagg tatttgaaaa tcgaaagcac ttcctgcagc attttctttt
     5101 acccctatag gaatactatc tgttcgcaaa ggagcataag cttcgctatt gagccaaagc
     5161 tcatgttgat cgattgactg agaagcatct cccgtcaagt tgaagtaaac atgatttgtc
     5221 ggattgaata aagtatcttg gtcactggtg ccgcgagagg tcactcgcca gatattatct
     5281 tttgtcaaag tatagcggat ttccacatcc attgtacctg gaaaaccatg ttctccgtcg
     5341 ggacttgtag tagtaaaaat gacagaagct tcattttcgc cattcaaaat gacataagac
     5401 cattttttta attcaaatcc aggagcacca ccatgcagat tatgtccagt ttttggatct
     5461 gtggctaatt ggtaagtttt tccattgaga gaaaagcggc cattttcgat tctccctgcg
     5521 gtgcgaccga tagaagcacc gatatacgga tctttttcga tatattcttc agcggtgtca
     5581 aatcccaaaa ctaattctcg atcatttgat tttaaactga cgattctagc acctaaatca
     5641 gagatagaaa gtgctagctt gtttttgttc tccaaggtta tcaagtgata accttggcca
     5701 aaatcttttt ctgtaatctt cattttcaag atcctttctt aatgggaagg aactactgca
     5761 tcgtgaagtt catcttccat tgtatcagct ggagccaatt tgttttccaa ttcctcaacg
     5821 atttgtgcat gttttttctc tgtcaatgta atcttccaag caaaaataat agcggagaaa
     5881 atcatcaaag cagcaggagc atagaatgca taagctttga aagtcgttac gccatgagca
     5941 gtgatatcag cagcagtagc attaccagtc atcccagaag cgatagcgac aaaccctacg
     6001 atcccgttag acaaagcacc agcgatttta tctagtaaag ggcgaattga tagagtaaca
     6061 gcttcgttac ggataccatt tttccattga ccatattcaa cagagtctgt gatcgtcatc
     6121 aaagcggata agaaaatcaa ttgttgaggg aagtagaaca atactagccc gatgataacg
     6181 attggcaaac ttgaacctgc gatcgtaaag aaaaagtaag aagaaatcat cattgcgatc
     6241 cctccaaggt aaacgtagcg acgagagatg acgcgtgtaa gcattgggaa tagtggaaca
     6301 gctactaaac caagtaaggc agcaactaga ccaacgatcc aaaattcagt tggttggcca
     6361 agtacatatt tgaagaagta gaataatacg gcagttgttg ctacattggc aatcgcataa
     6421 gcgatataag caagggcaag ccacatcaac tgatcatttt gagcaactgc tttgaacaca
     6481 tctttgatcc cagctttttc attttgctga cggatcgcag tatcgacttc ttttgtgccc
     6541 aaggcagtga taatcgcagt accgcctgaa ataacagcag caatcacacc gaaccagaac
     6601 cagcctgaag cgccttcaat atgtttatga gtaacaagat aggtaaagaa agtaacgata
     6661 gggacaacga ctagagtagt accatttgca cctagagaag agccgaaacg agcaaacgta
     6721 gcaaattttt cacgttcctt tgtttccgta ctcaaagctg gaatcattga ccaaaaagcg
     6781 atatccttaa atgaataaaa actatccaat aagatgaaaa cgattgcaaa taatatgata
     6841 aataaagtcg tattatgagt agctaagcca aagaaattgg taaagattgc taccaagagt
     6901 acggaactaa caacgccgcc gataactaac catggtttga atttaccgta ttttgtacga
     6961 gtattatcga tgatgccacc aatcaaagga tcaaaaataa tttcaactaa gcggatacct
     7021 acaactaagc ttgtaactgt agcaatcatt tttgcattcg ttgccttatc tgttcctgcg
     7081 aacatctggc ttgtcacgaa taccataaaa taagtgctta atgtaacata gaatgtgtcg
     7141 tgtccaaaag cgcctaaagc gtaggacaca cgttggatca ttttgctttt cataatctgt
     7201 attcctctcc tgttcaatat ataatactaa gtaaaccggt tacaacgaat aacaaagatc
     7261 ttttaatgac ttaaacttaa caagaaatcg cttccttttt aatatatttc tgtgactttt
     7321 atttaacttt ttgttgcatt atactaaagg agtaaggagg gatagacgtg gagcaatcga
     7381 tttttttaaa aaaggaacat caacaagtag aaaaagaatt gtattttgat ttttgtggtt
     7441 tctctaaaac gatgccgttt cattcttttg gtcctgctat tcgcaatgat tacatcctgc
     7501 atgtcgtgat ggaaggcaaa ggatcttatc atgtgaaaaa tcaacaattc caattgaaaa
     7561 aaggtgatct gttcttgatt cgtccaggag actcgacgtt ttacttatca gatggagaag
     7621 atccgtggat gtattgctgg ctttcatttg gtgggagtgc tgcagaagaa atcattcggc
     7681 agtcgctttt cagagaagac caatacacga tggtctcttc tgacatacaa aaatatatcg
     7741 acattatttt agaatgcatg aaagatacgg aagaaacctt ggaaaatgaa ttacagttaa
     7801 cagcattaac ctaccgtttt ttatcagtcc ttttaaaaga tggcggaaag gtcagcggtg
     7861 gtgtacaaaa ggttcattcc tctttagctg ttgaaacagt caaatacatc gaggcacatt
     7921 atgctgaaaa attgtctgtt gaggaaatcg cccagcattt agcggtcaat cggagccatt
     7981 tgtcgcgagt attcaaaaat catatgggta ccagtatcaa ggaatatttg atcggggtac
     8041 ggatcaatcg aggggcgttt cttctttctc tgacggatga atccgtggaa agtattgcct
     8101 atcaagtagg gttcaatagt ctagtcgtat ttagccgaat gttcaaaaag agtaccggag
     8161 aaacggctac gaactatcgt aaacgtatga aaaacgaaaa ttttaagaat attgctttgg
     8221 ataccttgaa aaaacagttg gaaaaacaaa ccgcagtttc ttggacaacc tgattagtct
     8281 ggttaaaatg acaagcatcg gcaaaagaaa cttgtggcgg ttatgctaca ctgtaaagcg
     8341 aagactaaaa aggaggaaaa gggatgcgta cacttatcat cattagccat ccaacgatcg
     8401 aagaatcttt aaatcagcaa tttttcaaag aagcttctgc tggtttacca gctacttggc
     8461 atcatttgga atcaagttat ccagacggaa aaatagatat agagactgaa ctacagctct
     8521 tggcccagca tgatcggatc attttccagt ttccgtttta ctggtatagt tctcctgctc
     8581 atttaaaaat ttggcaagat aatgtcttgg aacgagcagc acatgtatta aaagggaaag
     8641 aactaggaat cgtattgaca acaggtgtgg cggaaaaaga ataccaagct ggaggaaaag
     8701 aagaatatac gatttccgaa tttttgaggc cgtatcagcg aatcgcaaat aaattccata
     8761 tgacctatct tccgccattc gttttagcac aatttatgta tttatcccag gaaaaacgtt
     8821 gggagaagct aattgcttat cagcagtatc tctcattgga aggcaaacca tcgctgactc
     8881 agcggattga ctggtttatt caaagagtac aggaaaatca gaaaatgcag gaagaagaat
     8941 ctgaaaaaca agcatatatc gtagaagcac tgactgaggc aaaagagcag atcgaagact
     9001 tgacctttac actgcaagaa atgaaaggaa cttcattatg acaaatgatt ggctgatcca
     9061 acaattagat gaattaagcg aaaaatcaga atatcaagac cgtgcgctct tatatgaaat
     9121 aaagaaactg atggctgaga tagacgaacg aacaatgcag ctgcaaggag aaatcgacgg
     9181 acaatcttgg aatcatctca actggtgaaa ttttcttata aataacaaag ataaaaatgt
     9241 gcacagcgac aattatttgc tacaataaga atatgaagcg tttgataacg ctattataca
     9301 taaaaattgg tggtgaaaaa aatgtatgat gaaaatgagt ggggagaaaa tgttcggatg
     9361 acccgaagaa gacagcaaaa acaggcagct cgtaagcgga tgatcatgat tggggcagca
     9421 gttgccgtgt ttttgatcat agcaggagga gttgcgggtc tatattttca tggaaaaaat
     9481 tcgtcagaag cgaaaagtac gaccataacg gaaacaacga caaagaaaac agagacgaaa
     9541 caaacaagtg agtcatcaac taaaacaaaa gagacacaaa cttctgaaga aaaaacagca
     9601 aatggtttgc cagagcaact tcaagcaatc gtaaatgatt attcgaaaaa gatcgaagaa
     9661 aagacgcctg cattgattga agaataccag acagaaattc agggaaatca agagggaata
     9721 gctggtttat cagcagttgc taaccaaaaa gcacgtgagc ttcaagcaat ctccgacgaa
     9781 gggatcagaa aattacgtgc agcgtatcag gcagcagaaa ataaagacgg tgtagactta
     9841 gatactttga tcaatcaatt atcggctaat tatacgaatc atgtcgctaa gatttccgat
     9901 atttatttgc agactagcgc ctctcttcaa gcagagtcaa caagcacaca agacacaaca
     9961 tcttctgatt ctgagacagc agagtcggta gaagcttctg aaatgcaagc tcgaacaaca
    10021 caagacacaa cagattcctc tgaaactaat accaacagcg cgacttcaca ggctgcgaca
    10081 accgtagttt tggaaggaga aggtccaaat caaatagccg aacgaactgg cgtaccagta
    10141 gaaacgatct tgtcgctgaa tgggatgagc atggagaatt atttcctgac tcccggtgag
    10201 acgctaaaat tgaattgata aaagcaataa aaaagaacga agcttccact aaggtgaagc
    10261 ttcgttcttt ttttattttc cggttctttt ttcgcgtaca aagtaatgga tcgtcatgta
    10321 aagcaagaaa acgagtccaa taatcaatga gattctcgtt tcaggattga taaacatgaa
    10381 aatcaatacg atagctaaaa aagcaagtgt cacataattc gtataaggag atagtggcat
    10441 tttaaaagga tgatcatgca ttttatctgc gtgcttcttt ttgaagcgaa tctcgctgat
    10501 caaaatcact acccaaggaa ccataccagg caaaatactt gagctgtata cgaaaacgaa
    10561 aatattttct gactggttga tgaacattgg tagaagataa tttaagatca gaccaattaa
    10621 gattccacct gagattgcta cgacaggata aaaaggcgca ccgtgacgag atagtttcag
    10681 aaatttttta gacatctttc catttatggc aagtgtatag accatacgac ttgcgctgaa
    10741 aatccctgaa ttgcaacctg ataatgccgc tgtaatcacg acaaaattaa tcagtcctgc
    10801 agcaaatgta atgccgactt tagaaaaagt ttgtacaaaa ggtgaaccaa tctcactcaa
    10861 ttgattccac ggatagatac tcacaatgat aaaaatagca ccaatataaa aaatcaagat
    10921 tcgaccgata gtggactgga cagcttcaat gatcgtcttt ttaggattct ctgcttcacc
    10981 agcagtcatt ccgatcagtt cgattccttg ataggaagca acaacgatcg ataatgcaaa
    11041 gaaaaatcct ttgacgcctc cagtgaagaa tccaccattt ttccaaagat ttgaaatccc
    11101 tactgcctga ccatgattac caaatccaaa taagattacc ccaagacctg caatgatcat
    11161 caaaatgatc gttatgactt tgatgatcga aaaccaaaat tctaattcgc caaacgcctt
    11221 gacagaaata agatttgcca gcataagaaa caagacgaca atgattccgg gaataattgc
    11281 cggcaaatca ggccaccaat aattcatata agatcctaca gcaatcactt cacttattcc
    11341 tacgacaata tattgaaaaa tattactcca agcggtcaag tagccagcca caggatggat
    11401 gtactcgctc gcgtaatttg caaaagaacc agtcgaagga tcaacgtaaa gcatttcgcc
    11461 taaagcacgc ataatcaaat acaaaaagaa tcctgcaatc gcataggctc ccatcactga
    11521 gggccctgtc caacgtatgg ttgaggaggc acccataaac agaccgacac cgatcgttcc
    11581 acctagtgcg atcatttgga tatgacgggc actaagactt cgagttaact ttttttcttc
    11641 catacaaaaa ctcctttcgt tgttgagtca acaagaaaaa ataatcaaac gtttcaacac
    11701 atgtttttca ttattaggtt agccttattg aactcttgat gtgattctac agaaaatttt
    11761 cataaaatgc gaggaaaatt cgaaaaaaac tttttcatca ttctacttga aaatgctttc
    11821 cattttagtt aaactatatt taaataaaaa gttaggtaaa cctaaaaagg agtgtaagat
    11881 ttggaacgta cggccattgt aattcaagac ctttcagccg cttatcaagg gaaaactgtt
    11941 ttgaaaacta tcaacttgac gataaatcca caaaaaatta ccggtattat cggaccaaac
    12001 ggtgcaggaa aatcaacatt tatgaaatct ttgctagagt tgataccagc tactactggt
    12061 aaagtcacct tttctggaca gccggtaaac tcagtccgta aaaaaatagc ctacgtggaa
    12121 cagcgaagtg aattggatct ttcctttcca attgatgtat taggcgttgt acttttagga
    12181 acatatccat ctttacgaat tggacaaaga cctgggaaaa aagaaaaaga acgtgcaaga
    12241 caagctttga aaaaagtagg gatggaagaa tatgccaaaa gacagatcag cgaactatcg
    12301 ggtggacagc ttcagagagt ttttattgca agagctctag cccaaggagc agaatggatc
    12361 tttttagatg aaccattcgt agggattgat gcgttaagtg aacgaaagat ctttgacatc
    12421 ttgcaggaat tgaagaattc aggaaaaacg attttgatcg tccatcatga tcttcataaa
    12481 gtagaagaat atttcgatga ggttattctt ttaaataaac agctgatcgc ttccggtcca
    12541 gtacaagaag tctttacatc gaagaacctt caattggcct atggtgagat catcagacat
    12601 ttagtaaaag gaggagagaa aaaatgatcc aatcctttat tgatggatta atgaattatc
    12661 aattcctgca atacgctttg gtcacttcca tgcttgtagg acttgcttct ggagtcatcg
    12721 gttcttttat tattttacga gggatgtctt tgatgggaga cgctatctct cacgcagttc
    12781 ttccaggtgt agccatttct tatatgtttg gttttagtta catctttgga gccactgctt
    12841 ttgggatgct aacagcagca gtgattggtt tcgtgactca gcggagccga ttgaaaaatg
    12901 atacagcgat tggaattgtc tttagctcct tttttgcatt agggattatc ttgatttcat
    12961 atgcccagag tgcgacagat ttgtatcata ttttatttgg gaatgtctta gctgttaggg
    13021 aaagcgattt gttgctgaca gcactcgtta gtggaatcgt cttgatattt gtctttttct
    13081 tttataaaga attgaagatc acatcattcg atccgacaat ggcaaaagct tatgggctga
    13141 acacatcgct gatccattat cttttgatgt tctttttgac attagtggct gtagtcagtt
    13201 tacagacagt aggaacaatc ttggtgattg ccatgttgat cacaccagcc gccacggctt
    13261 acttgctaac gaaccattta ctgaaaatga tcattacagc tgcaggaatc ggtatgctaa
    13321 gtgcagttgt cggtgtgttt ttcagttata gttacaattg gccatcagga gctacgatcg
    13381 tgttagcatg taccgcattt tttatccttg cttttttatt ttctccaaca aaaggaattt
    13441 tatttcagaa aggaagcaga tagatgaaaa caaaaaaatc actcttttta atcttggcag
    13501 ttagtttctt ggtattagcc ggttgcggaa agcaggcttc tgaccaagcg gatgaaggaa
    13561 gtaaagaaaa gttgtcagtc gtggctacca attcgatctt ggcggacatg gcaaaagagg
    13621 tcggaacaga tcaaatagat atccacagta tcgtaccagt cggaacagat ccgcatgaat
    13681 atgaagtact accagaagac atcaaaaagg caagtgatgc agatgttatt ttatacaacg
    13741 gtttgaatct tgaaacaggt aacagctggt tcgataactt gatggaaacg gctaaaaaag
    13801 aagaaggaaa agattatttt gcagttagca aaaatgtaga acctctttat ttaactagcg
    13861 gtgaagaaca tacaaaagca gatccccatg cttggctaga cctatctaac ggaataaaat
    13921 atgtggagga aatcgcacgt atattctctg aaaaagatgc agaaaatgcg acactctata
    13981 aaaaaaatgc agaagcatat gtggaaaaac taaaagaatt agatacacaa gcgaaggaaa
    14041 gttttgcttc tatcgaagag aacaaaaaat tattagtaac aagtgaaggg gcattcaagt
    14101 atttttcacg agcatatgat ttgccagcag cttatatatg ggagatcaat acagaaagtc
    14161 aaggtacgcc tgatcaaatg aaagcgatca ttgatcagat aagagcaaaa gaagtaccgg
    14221 ttttattcgt ggaaaccagt gtagactcaa gaagcatgga acgggtagca aaagaaacgg
    14281 gtttgaaaat ctatgataaa ctgttcactg attccatagc aaaagaagga gaacaaggag
    14341 attcttacta tcagatgatg aaatggaata ttgaaacgat ccatgaaggt ttgtcacaaa
    14401 caaaagaaag ctgaaaagtg caaggtctag cttgaggagc agaccttgca ttttttataa
    14461 gagatatgaa aaaaaattat gctgtttttc gtcgtacaga tacctttttt gaagaaattc
    14521 cgttatagtt agagtaagga aggaaggtgt ggctatgaat tttacacgta aagataaata
    14581 tacgattaaa gaagaagtct ttcgattgat cagccagcag gaaaataccg acaaggatca
    14641 agaccttcaa tttcttgaag cagaattagc taaattattc atagcaaatc aaaatgagac
    14701 aacagaacca gatgaagagc tgtacatagg caaatggttg tttgatgaat ttcagccttt
    14761 agatgaaaat accggtatcg gttattttgt gttcctttct ccagtcggct tcacacctgg
    14821 cgtattagtt aaacaaaacg acgaaataac cgtcaaagca tggcctgcat tattttcagt
    14881 catcaaagca gaaggagtaa tcaaaaagat ccagtcggcc tattataaaa gtcaatttat
    14941 tgccaacagc aaattaatga atatgtttta taatcgtcta gtagaaattg ctaggcctgg
    15001 gaaaatcccg caaacattca cgtacacaca gcaaaaaaat ctctatgaag aagtattgta
    15061 ttcccgcgat ctgtctgagt tttcgccatg ggaagaggta aggctccatg aactgttaag
    15121 tacaaaacca gtttcattga atttttttaa tgcattagat aacaaaagaa aattaatttc
    15181 aacaaattat tgggaagtat ttgctgcaag aaaaaaatgg atggaagaaa agaagattgc
    15241 cgatcagcag atccagtttg tccagctgaa aaaagaaaaa ctatattatg ggttatacag
    15301 ccgttaacag caagcgagga agaccatgac attaaaagaa gaaatcatac aagaaagcaa
    15361 acgattagga attgacaaaa tcgggttcac cacggcggaa ccttttgatt cattaaaaga
    15421 atcgttggaa gaacaaaaag cagctggcca tacttctggc tttgaacatc caaatatcga
    15481 tgagcgtctt tatccagaaa tgacatttga tcagccgaaa tcgatcattt ccattgcact
    15541 tgcgtatcct actaggatcc atgaagaagt gcctagggat gaaaaacgcg ggcagtttgc
    15601 tcgtgcttct tgggggatcg attaccatga tatactcaga gaccgtttga accgattgat
    15661 cgaattcatc caaacaagag cagaaaaatg gcaaaaagaa gaggaatggc gtctagctcc
    15721 gcaagtagat actggagaac tgatcgatgt cgctgtagca cagcgagctg gtttaggatt
    15781 tattggacga aacggattgt tgatcactga agagttcggt tcatttgtct atcttggtga
    15841 gatcgttact aatattgtat ttgagcctga taaaccagga gaatttggtt gcggtgactg
    15901 tatgcgctgc gtgactgctt gtccgacaaa agcattgctt ggtgacggaa gaatgaatgc
    15961 ccagcgatgt ttgtcttatc aaactcagac aaaagggatg atgccggaag aatatcgtaa
    16021 aaaaatgcga aatgtcattt atggatgtga tatctgccag ttagtctgcc cttacaatca
    16081 aggaaaagat tttcattttc atgaagagat ggaaccaaaa gtagacgaag tatatccaaa
    16141 attaaagcca atgctttcga tgtctaataa agacttcaaa cagcaatttg ggcatttagc
    16201 tggttcatgg cgtgggaaga aacctttgca gagaaacgca ttgattgctt tagctaatct
    16261 aggtgatcga acggcattgc ctgagatttc agaatgttta acagatgtcc gccctgtcat
    16321 cagaggaaca gcagcttggg ctattggacg tctaggaaca aaagaaccgg aaaaatggct
    16381 tgatgtatta gatgaaatat tagctaaaga aacagaagaa gtcgttataa a
//
LOCUS       DACDEG010000053        15940 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.055, whole genome shotgun sequence.
ACCESSION   DACDEG010000053 DACDEG010000000
VERSION     DACDEG010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.055"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            21..1448
                     /locus_tag="IV142_001755"
     CDS             21..1448
                     /locus_tag="IV142_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8485415.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1540..1905)
                     /locus_tag="IV142_001756"
     CDS             complement(1540..1905)
                     /locus_tag="IV142_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP8485416.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(1902..2267)
                     /locus_tag="IV142_001757"
     CDS             complement(1902..2267)
                     /locus_tag="IV142_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP8485417.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2459..2707)
                     /locus_tag="IV142_001758"
     CDS             complement(2459..2707)
                     /locus_tag="IV142_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485418.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2706..2834
                     /locus_tag="IV142_001759"
     CDS             2706..2834
                     /locus_tag="IV142_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP8485419.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2891..4585
                     /locus_tag="IV142_001760"
     CDS             2891..4585
                     /locus_tag="IV142_001760"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP8485420.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4685..4858
                     /locus_tag="IV142_001761"
     CDS             4685..4858
                     /locus_tag="IV142_001761"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485421.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5236..6369
                     /locus_tag="IV142_001762"
     CDS             5236..6369
                     /locus_tag="IV142_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP8485422.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6400..6720
                     /locus_tag="IV142_001763"
     CDS             6400..6720
                     /locus_tag="IV142_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485423.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6749..7213)
                     /locus_tag="IV142_001764"
     CDS             complement(6749..7213)
                     /locus_tag="IV142_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8485424.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7345..8598)
                     /locus_tag="IV142_001765"
     CDS             complement(7345..8598)
                     /locus_tag="IV142_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8485425.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8627..8944)
                     /locus_tag="IV142_001766"
     CDS             complement(8627..8944)
                     /locus_tag="IV142_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8485426.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(8970..9290)
                     /locus_tag="IV142_001767"
     CDS             complement(8970..9290)
                     /locus_tag="IV142_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8485427.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9754..11169)
                     /locus_tag="IV142_001768"
     CDS             complement(9754..11169)
                     /locus_tag="IV142_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8485428.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11186..12502)
                     /locus_tag="IV142_001769"
     CDS             complement(11186..12502)
                     /locus_tag="IV142_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8485429.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12652..13350)
                     /locus_tag="IV142_001770"
     CDS             complement(12652..13350)
                     /locus_tag="IV142_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP8485430.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13639..14628)
                     /locus_tag="IV142_001771"
     CDS             complement(13639..14628)
                     /locus_tag="IV142_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP8485431.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14708..15541)
                     /gene="msrA"
                     /locus_tag="IV142_001772"
     CDS             complement(14708..15541)
                     /gene="msrA"
                     /locus_tag="IV142_001772"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8485432.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
BASE COUNT         4960 a         3224 c         2719 g         5037 t
ORIGIN      
        1 tacaaggcgg aggctctact atgattgaag aatatattga gaaaaatatt ttaagacagc
       61 tgtttttatg tgggcaattc tatgtaaata aagaagtcaa tctagaaaag ctctccaatc
      121 tcctgcatgt ttgcaaaacc actttactca atgatatcaa taacataaaa aaagaatttg
      181 aagaacagat cgcttataca catcgggaaa aggaccgtta cactttatat ttttcagaac
      241 atatacctcg atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt
      301 gtctgcttta tttggaagaa gatgaacctg attatcttca attaactgaa tgtgaattca
      361 tctctgtcag caaagcttat tcattaaaaa aacaagtatt agcttacttt aatgactgcg
      421 ggatcgaaat cgaccgctac tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc
      481 ttaatgtgtc gtatcgttta ggcggtttta attcttggga gctacctgaa tcgttttttg
      541 aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag tggcagattt tatgataaag
      601 aaaataaaga aatcttatcg attggttttg ctatcagttt cctacgccag caagtgtgtg
      661 ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt tacaattatg
      721 tcgaatcagc atgggaaaac actgatttcc aaacctatta taaaaaagaa gaattcgcct
      781 ttattttgac actattcaat ctttgtaatt atggtttcca ttcttatcag ttgattgcgg
      841 aagactttca acagctacac caagtgttta ttgacaatac tcccgaaata aaagaactag
      901 ttgctacctt tgaatctcac ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg
      961 cgctgattca tctgatgcgt tcggcttggg ataactatca attattcatg ccagaaaaat
     1021 tttatctttt aaatgaggaa cagacaaatt tatataaaga agtccagaca attttttcca
     1081 gctggtccag ccagttacct tatgatctac ggttgaatcc taattgcatg cgggcttttg
     1141 tgatcgaatt gagcggaatt ctccgtctga caaaagaaca tctgactatc tatatcgtca
     1201 caaattcgga tgttcattat ctgatttatc gggaagcctt ggaagctgta actactttcg
     1261 attttcaagt tgcaccaaca atttattcaa gtatatctga tataaagaag tatgcgcagc
     1321 agtcctcaaa ccgggtatta tgtgaacgta cattatatac accagatgcg gtccaatatg
     1381 aaaatattat tcctatttcg atcaatacga tcgatcgagc tatcatttca gctgtgcaaa
     1441 acaaataagg ggctgtgaca aaaatcatgt cacagcctct tatttgttga agatgcccat
     1501 ttctgtaaca atctcttgat acacgtcagg ctcacaacct cacttatttt ggacatctag
     1561 gggtccgatc tctttattta aaaaataaga aatgatggtt cggataacta ccaacgccgc
     1621 tagttggaaa atatcctgca aggtaggttt gataatcgat tcgatgatcc cagctgaaac
     1681 aagaatttcc aaacttagaa gtatatagcc tcccaacatc tttttaatag cattgttctc
     1741 gcttactcgg tcgctttgca cttttgtatg gacaaataaa tatttcaaaa aatccttgat
     1801 tgatagacaa acaccccaaa ccaataaaca gatagcaaag atatcaagtc ccagtacaag
     1861 tttttcgaaa tatggaagaa gaaactgcat caactctgtc gtcatcctac ctctcctctt
     1921 ctttgttttt ctttgtcttc tatttcctga ttcaagaaat aagaaatgac tgttcgaatg
     1981 ataataataa gtgcaagctt caaaatatct gtccaagtcg gcttgataac tgactcgatg
     2041 atatcagcaa cgactagtac ttccaagctt aacaaaatat aacttgctag cagttttctt
     2101 gtatctgcat tacgagtatt acgttctgca tagtttttct tgacatgaga ccaatcaaaa
     2161 agtttgaata gaacaagaac gatcccgaca attaagatac cgatagaaca aatatttaaa
     2221 atagcaatga tccattctag aaattcaatt aaatttgagc gaaacatata cgctacctac
     2281 tttcttttaa tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt
     2341 aaaagttgtt ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc
     2401 tccctccatc cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt
     2461 agtccgtcat cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc
     2521 taaaaataga accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta
     2581 caggaaaatc agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca
     2641 tttggcattc taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt
     2701 ttttcatgag taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg
     2761 ctgcatatct aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat
     2821 tcgaatcaat ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg
     2881 aggaatcaca atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc
     2941 agcggctgtt tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc
     3001 attggatgat cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg
     3061 gaataaaaac catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct
     3121 taattaccgt acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga
     3181 gccagggatg actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga
     3241 caaagcacgt ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa
     3301 taataaagac cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa
     3361 actagacaat atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa
     3421 cttctggttt atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt
     3481 gcgtcgctat atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt
     3541 caatcgaaca cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa
     3601 agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac
     3661 accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga
     3721 agaagaatac gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac
     3781 agattcagct actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc
     3841 agcttctagt ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa
     3901 attctttgct gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca
     3961 tttgttgttg aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc
     4021 atttatttca accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctattgt
     4081 cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta
     4141 tttcctttat ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg
     4201 aaaagaaatg ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa
     4261 tattcgtgaa aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat
     4321 gccttatgct tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc
     4381 taaacattcg accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat
     4441 ctttactgaa caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat
     4501 tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg
     4561 tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt
     4621 aataagaaca accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc
     4681 gcagatgcaa gcttttgaac accgtttacc aagaggttat gaaaaagctg ccctgcatca
     4741 agtaataaga gcagccaagc taaaacagct tcttatgttt ttgacttggt tagacttatt
     4801 accgcagatg caagcttttg aacaccgttt accaagaggt tatgaaaaag gcgtttagtt
     4861 tcgagtatga agaaaaattt tgtgaaaatg gttcctcacg tttttgcata aatttgactt
     4921 aataccgaga aactgatccc ttctgtcata acctcttttt atttatgtct gtttcttttt
     4981 gaaaacaact attttttcaa cgtttactct cttgccaatc caacatcatc taacggatac
     5041 aactatttca atgattacca ttacactaaa aaatcggaaa cacacctctc cgaacacaaa
     5101 tacataagac tatagaatgt ttcatttaag aaaaaaagcg tttcattatc aaaaaggtaa
     5161 gatctccttt aaaatgaaat tatctgtagt actaataatg cagacaattt tatgaatctg
     5221 aaaggagcat ctccaatgaa agcagtcact tggcaaggaa aacagaaaat ggaaatcaga
     5281 aacgtagatg atccaaaaat attagaacca acagatgcaa tcatccgtat cacagcaaca
     5341 gccatttgcg gctccgatct gcatctttac catcatggtg aagcagtttt ggaaaaaggg
     5401 tatgttgtag gacacgaacc aatgggaatc gttgaagaag tgggatctca agttcaaaca
     5461 ttgaaaaaag ggcaacgtgt ggttatccct tttaatatta gttgtggtca ttgtcatttc
     5521 tgcctgaatc acatggaaag ccaatgtgat gaatcaaatc cagatcagtt atatggcggg
     5581 cttttcggat ttggcaagtt gaacggtaat cattggggtg gtcaagcgga atatctaaga
     5641 gttccttatg cagactctac ttcatttgtt gtaccagata atgatcttcc tgatgaaaaa
     5701 gttttatttc tatcagatat tttgcccaca gcttattgga gcgttgaaaa cgcaggagta
     5761 aaaaaaggcg atacagttgt gattctaggt tctggtccag tcggtctatt cgctcaaaaa
     5821 ttcgctgtta tggctggagc agagcgcgta atcgcagtag atccagtaca gcaccgtcta
     5881 gataaagcgg ctaattacaa tggggttgaa acttatttct tagaagacac ggcacaagct
     5941 ggaacagaat tgtatgaact ggctaaaggt ggcgcagatg tcattatcga ttgtgtagga
     6001 atggacggat tagagcctgt caaagaaaaa gcgaaaaatt tagtaagctt acaaagcggc
     6061 acaatctctc cattacagat ggcatcgcaa gctgtcaaaa aatttggaac agtccaagta
     6121 actggtgttt atatgactcc agcttcttct tacccgctgc aagaattttt tatgcgggat
     6181 atcgctgtca agcatggaca ggcaccaatt atccatctaa tgccaaaaat ttacgcaatg
     6241 attgcagaag gactattcga tcctacacaa atcattactc attcgctgcc actatcagaa
     6301 gcagcacaag catatgagat ctttgacaaa aaagaagata aaaatataaa agttgttttg
     6361 aaaccttgaa ttcagtaaaa aatacaggtg attttcttta tgcccaaaat caaacgaaat
     6421 acccatgaga tcgaaatagg aaaagaagaa gatatcgaaa tacgaggacg tcgcttccgc
     6481 ttctatttcc aaaatcgtta cacattgatc tcactggctg tcgatttatt aaccgggatt
     6541 ttctatattt ttggaagcct tgcttcctta actaaaatcc ccgatcgata tggcacctat
     6601 ttttatttgg ctggtgctat tttcttgacg attcgtccta ttctcagaat cgtaaggaac
     6661 atttttattt acgacgaaaa aaaagcaatg aagagaacaa gcaaaaaaaa agaggcatga
     6721 gaatttttca aatctcatgc ctcttttttc accctatcgt atcactttga tacagacgag
     6781 cggtcgtccc actttcttcc ttgaccattg tcaaaaatgt ccactcgtat cgttcataaa
     6841 acgtggtatg atctgtaatt aagtaaagtt tttcgtaccc aagttgatgc atttcttttc
     6901 tgatttccgc taatatccgc ttggctatcc cctgcttacg cttttccggt tcaacaaaca
     6961 gggcacaaag atttggtgtc agatcagggc ggttatggaa gtcgttttca atgacgcccg
     7021 ctcctccgac gatggtttct ttttcatcta agaccacata ccattgagga atactttcct
     7081 tattagcgat tccagtacga atactttcct catacacaac agcaggaact ccccattttg
     7141 atgaaaacca attcgcgctt tgttttacta attctgggtg ttctcttaat ttgatccagt
     7201 tatatgtaac caatgtttaa acctccgact aaaataaaac gtttacaaaa attatacaag
     7261 tctttatgaa aattagcaat gacaacaata gtgatgtaaa aaaaaaaacg gaaaacaatc
     7321 acttgttctc cgttttttga ttaatcaagc atctaaagct tcttttcttc tttcttggat
     7381 cgtagctacc ttgacgaacg gtagatagat caaaatagaa agaaccaaat tgacagcggc
     7441 tagtacacca cctgcaatac tttttgttgc caatacaccg ccaataattg gtggagttac
     7501 ccatggaggc ataacggtag ctgctggcac taaacctgta gaagtcgcaa agtaagcaac
     7561 actaactaag accattggtg tcaaaataaa tggaatgaac atgatcgggt tcaacacgat
     7621 aggtaaccca aacatcgttg gttcattaat attgaagaca cctggcgcaa tcgacaagtt
     7681 tgttacaacc atatatggtt tgtttttacg tcctactaaa taaatagcta acagtaaacc
     7741 taaagttgca cctgttcctc ctaagttgac aaagctgtca aagaaaggtt tgttcacgat
     7801 aaacggtgcg ctatgtccag ctgcgattgc attaacgttt gcttcaatgg ctggcgcatt
     7861 gattgtttgc atcaaaggat cgaccatatt cgctccgtgt aaaccaaaga accataagaa
     7921 tggtgtgata aatgcaagca gtaaggcaga tggataagag tttgcaagtc ccataaatgg
     7981 ttcttggacc actgtataga aagaacctac gatatcatca acaccgaatg cagcaaaaat
     8041 tgcagcaact aaagcaaacg cagagatagt gatcattgct ggtagtaatg aagcaaatgc
     8101 ttttgcaact gccggtggca caccatcagg cattttgatg atcaattttt catttccgct
     8161 taaacgtaca aagatttctg ttgaaataat cccaacaata atagcaataa acaaaccgtt
     8221 tgagctcata cctaatgctc cgcctaaagc gaagaaagag cctaaagaaa tcacacctgc
     8281 ggaaacaccg tcttttcggt agccgttagc taaatgataa gcaagcaaga aagcaatcaa
     8341 gacagaaaga atggcaaatg taccgttcca agcagcagcc ccgaatcctt tccatgattc
     8401 accaccaaaa attgagttca tcaattcttg gaacccggga atcggtaagt tattgatcat
     8461 tactgctaat gctcctaaga tcattaacgg catactcacc ataaatgagt cccgaatcgc
     8521 aactaaatgc cgttgtgcac caatttttga tgctacaggt ataaaatgtt tctccataaa
     8581 tgcaacaaat ttatccataa atcttccctc ttttctttac tgtagattac gcgttcaatc
     8641 gacgatacag atcaatgatc tcttttgcta aatctgtaaa agctatagat gtcatcagat
     8701 gatcttgact gtggaccgtc aaaagcgtga cttcaacgtg atttccttgt gcttcttgtg
     8761 tcagcatctc tgtttgagag tgatgtgctt ggactaaaga ttcctctgca tctttgattt
     8821 tttcatctgc taaagtgaaa tcaccttttt tcgcagcagc aatcgcttcc attgcatcgc
     8881 tttttgcatt tccgccaaac atgatcagac ccatgatggc ttctagattt ttttgatctt
     8941 ccatttattg tatcctccga taatttttat tatttgttta ttagtttttc tgcttggtcg
     9001 agtacttttt caccattcat catgccatag tcagccatat tgattacatc taaaggaatg
     9061 cctttaggtg ctagcttttc agcaaattga ttttgcataa agcgtacttg cggtcctaaa
     9121 agtaaaacgt ctacattttt agattctagt tgattatccg catctgaagc agatacagca
     9181 aagatgtctg cttccattcc ttttgcttct gctgcctttt gcatttttgt gactaggaga
     9241 cttgtactca ttccagctga acatacaagc atgattgttt tcttttccat tttaaacacg
     9301 ttcctttcta tacttacact tgtatataag caagtagcgt gccaacttta aaagcgcttt
     9361 aaaaagttga tatcatcaca tttaaggtct ataccaagtt gcatacacac taaaaaagaa
     9421 agcgcacact aaacacacaa attaattttt attacctttt ttcttttaac gtgcacaaaa
     9481 aaagttgtga cacaaaactt gtcacaactt ttttcaaata aacggtattc aaaagcttgc
     9541 atctgcggta ataagtccaa ccaagttaaa aacataagat gttgttttta ctttgctatt
     9601 cttattactt gatgcaggac agctttttca caacctctca ataaacggtg ttcaatcagc
     9661 tgaccctggg tattagctca aaacgctgga ttcacaacct cttttatttc aaataaccag
     9721 taaatacaat gaacatctac aaaaaacttc tatttattta tagatttcgt caggcaaatc
     9781 caattctctc ttatcaatca aagtctgata ccacgaagca gattttttca atgttctgct
     9841 tcgttcatcg ttcaattcga ttcgaactaa tccatatcga ttcttaaatg cattcattgg
     9901 tgacacacag tcagtgaatg cccataacat atagccttcg caatttgcgc cttcttctgt
     9961 tgctttgatc aagtaatgca aatgctgtcc gataaattcg atacggtaat catcttgcac
    10021 gattcctttc tcatccatat actgttcttc atattctctt cccataccgt tttctgttat
    10081 cagccaaggg atattatgat aattttcttt tagatacatg gctatatcat acatgatttt
    10141 cggatagatt tcccatcccc tggaattatt cattttcttc ccaggtaaat caaagacttc
    10201 ataatatcgt tcaggatgga aaggtgtctc ttcattccat tgataacgag gggcgcgaac
    10261 gcgtttagga taatattgat tgattccaag gaaatctact gtatgatgct gaatcgtttc
    10321 taaatcctct gttgcaggat cgaaataaat cgattgtttt tgacacaact ccaggaactc
    10381 ttctgtgtag tatcctttta ccatcggatc aaaaaatact cgattataaa acaaatcata
    10441 catataagca gctttttggt cttctggaga attggatcga gcatagacca tttcaggatt
    10501 taaaatacag ccaatccttc caccataatt tccgctgcgg taaatctcta ctgctttagc
    10561 cgttgctaaa actttatgat aattccacat catccacttt tttgtgtttt gttcatgcgg
    10621 ccagcgaaca gcatccaaat agcaacgcgt ttggggaacg attggttcgt tgaatgtaaa
    10681 ccagtttttt accctatcac cgtaacgatc aaaggcaatt tgagcatact tagcataaaa
    10741 atcaactact tttcttgaag accagccatc atatttttcc aaaagaaaag ctgggacttc
    10801 ataatgttcc aaacaaatca tcggttctac gtctgcttcg atcaaagagt cgatcatttc
    10861 acttatatgc tgagcatatt cttcatctac tatacagttc tcataatcag taaaaaaacg
    10921 agcccaattg atagaagtcc gataatgtgt cagatggatg gatttcatca gatccacatc
    10981 ctgctggtat tgttccataa aattagttgc tacggctggg ccatacccat tatgccaaac
    11041 aaaatgttca tttttatacc agctatctat aaacgaatcc tgtccttctt tttttccttt
    11101 ccaaccttcc gtttgccaag cagaagatgc agcaccaaga ataaaatttt ttggaatagt
    11161 aattttcatg atttcctcct gttttttaga tcccatcatt ttccacacga tttgctactt
    11221 ttacaaatgg cagatagatc aaaatagaaa caataataca aacaagttgc gtaataactg
    11281 caccgatatt tcctgctgtt gccagataag cattgacgat cggcggcatt ggccaaggga
    11341 ccattacaac tgctttcgcc gcaaatccta aagcggttgc aaaataacca atcgttccag
    11401 tgataagagg cgttaagata aacggaatag ctaaaattgg attcagcatg atcggcatac
    11461 caaaaataac tggttcattg atgttgaaga tcccaggagc aagggataac ttggtgattt
    11521 gtttcatatc ttcccgtttt ccaactaaca atactgcaat cagtaaaccg atcgtgactc
    11581 cagacccgcc catactcata tacatatccc aaaatggcat agtaataatg ttcggtactt
    11641 cttttccagc ttcaaaagct tccgtattta cagcaattgc cgctaaaagc aacggttccc
    11701 gtatcggctt caccatttga tttccatgga taccaatcac ccaaaagact tgcgcaataa
    11761 acatcaatag caaaataccc ggtaaccctt gtacgacacc ttctaatggg cgttgtacga
    11821 cattgtaaat gatatcataa aggtacattc ctgtaagttc tttgatgata aatcccgcag
    11881 ccgcgatacc cgatacagtc agaatcgtcg ggaataaagc agaaaaacta gcagaaacat
    11941 tcgttggcac ggtatcaggc attctcagct gtagtttctt ctgtttcctc aaccagcaat
    12001 aaagttcaat agaaaggatg gagaccaaca ttcccaaaaa taaccctttc gtatctgtat
    12061 attgtttagc caatacgttt tctactataa cagtctgatt ttcattcact aattcataaa
    12121 cagttggatt aacagataaa taagacatca ctgcgatgat accagggaat acaccatcca
    12181 ctttattcag cttggctaat tcgatcccta tcagaaaaac agcatagatg gtcaagaaac
    12241 ttagtgtgac atagctgatg gcagatgcga taggctttaa ttcttctaaa aaacgcaaga
    12301 aacttatctg tgctaagcca ttttccgagt caaagaccat tgctgaaaac aatgtgccaa
    12361 aagcacccgt aataattacc ggaatcaatg cagcaaatgc gttttttatc accatgatat
    12421 accttaaatt attgattctc gttgccacgt tccctaaaac attggctaat ttatccataa
    12481 aactcattcc agatcccctc attcctgctt cttctccttg acgtcatctc tttttgtcta
    12541 ttagtcagcg cttacatttc gatgaacaga aactatttac tcctcttttt taccaaatat
    12601 aaaacaaaaa ttcatctgat gtatttatca taaaatacat cagatgaact gtcaattagt
    12661 ttctttttct cctattattt tctgtaattc tctccgattt attgccatat ggacagtcat
    12721 tgcatcaaac gcagcaagtg cattccgcct ttcgatggcc tgaacgatct ctgtatgaat
    12781 atcgatcgtc tcgattttca gatgctggat atcataattt ttattaaaca aagcaattga
    12841 ttcattaatg attgggataa tatgcagcaa ggctacgttt ccgctagctt ctgcaatcat
    12901 cgtatgaaaa tcgatgtcta attgaacatg ttcttgtgtg ccttctcgaa aagaagtttc
    12961 aatcttttgc gttaattttt tcatttggac gatttgattc ttcgttgcat attccgctgc
    13021 caaacctgcc atcttaggct ccaataaata tctcagttca aataagtctc gtatcatccg
    13081 atcctgatct tcaatcaaag aaaaaccgag tgggtcattg gcaatccctc gcttatcact
    13141 gacaaatgtc cccgctcctt gccgaacttc aaggatattc ctggaagcta gagcccgcac
    13201 agcttctcgc accgtgcttc gccccacctc taaataatcg gacaattcat attcattcgg
    13261 cagtttatct cctacttgca actgcatttc ctttaaatat ctaaggatct tgtcagtagt
    13321 tacttctact agggttttat tttcttccaa accacatacc tccttttcat ctgatgtatt
    13381 ttttcgtaat tattataaca cgtttgaatc atctgatgat tttttgtata caaaattgat
    13441 taatccgttc atagacatac aaacgaagaa gatatttacc ataaaaaaga ctaggataaa
    13501 attcactcct agccttcgat catttttatt ttgaacgaca aaaaatatga tgttcaataa
    13561 cagacgatct tcattcgatt cttgatgtca tcttttgttt tttttaatat atggataaaa
    13621 agtttgatta catcgtttct attctagaat ttggtcgatt ctcaattctt cttctggtga
    13681 gaaatctaaa ttatccatca ttcgaacact ttcttgtaac tgacttaacc gacttgctcc
    13741 gaccagtaca cttgtaacag atttttgtcg caaattccat gcaagggcca tttgcgctaa
    13801 tgattgtcct cgtgatacgg cgattgtttg cagtgctttg attttttcca gagttgatcc
    13861 aacttgttct tctgaaagga atggtatttc cttacgatgt gctctagaat cttcaggaat
    13921 accatgtaga taacgatttg tcagcaaccc ttgagcaagc ggactgaacg tgattgcccc
    13981 tagcccttct tcttctaata ctggagatag atcgtcttct agtagtgcac gagaaaacat
    14041 attgtagcgc atctgatgaa taatgaacgg tgcttccttt ctttttaaaa tttcagtcat
    14101 tttttttgta tcttctccat tatagttgga tataccaata tataatgctt ttccttgtct
    14161 tacaagttgc atcaaagctt ctgctgtttc ttccaaaggt gtatcagggt ctgggcgatg
    14221 atgatagaag atgtcaacat aatctagccc cattctttgg agactttggt cacaacttga
    14281 aatgatggat ttacgagagc cccattcccc atatggccct tcccacatat gataacctgc
    14341 tttgctagaa ataatcagtt catcacggta ggcttgaaaa tcttctctaa aaatacgccc
    14401 aaagtttttc tctgcagctc cagcagggcc tccataatta ttcgcaaggt cgaaatgggt
    14461 aattcccatg tcgaatgcgc ctcttaaaat ctctctttga ttatggatag gatcataatc
    14521 accaaaattt tgccataatc cgagagataa aatcggtaat ttcaaacctg aatgaccgga
    14581 tttacgatat tccatttttt cataacgtgc actatctgca atatacaatt tttctcctcc
    14641 tcatcaaatc tttttaaaac gctttctttt ttctattttt agtttactac attttaaaga
    14701 aaaggagtta atcaaaatga ttttttcgta tatttttttg ccatttgttt tttactcgtt
    14761 gatagatcag aaattgatta cgtgcttgct ggattttttt ataacgtttc ggttgttttt
    14821 tgtaaaattg ctgatgatag ttttctgctg gccaaaacgt accggctggt tcgatttttg
    14881 tgacgattgg ttggtggaaa gttcctgaac tgtctagttt ctgttttgat tgttccgcta
    14941 attctttttg tcgttcgact tgataaaaaa tcactggacg atattgcttc cctctatctt
    15001 gaaattgtcc aaatgtatct gtgggatcag taatttgcca atagattgtc agcaactcgc
    15061 tataacttat ttctcttgtg tcataaatga tctccacagc ttccacatga cctgtgtaac
    15121 caccacttac ttgatcataa tttggttttt caacatgtcc gcccgtgtat cctgacaaaa
    15181 cagataggat cccttttttc gattcaaacg gctcgaccat acaccagaaa catccccctg
    15241 caaatactgc acgttcttga tagtcagcat cttgcatggc atgcttggaa aagtcatatt
    15301 tttgctcctt atcaaatcct tccgtaatca ataaataaaa gtccattaca tcaggcgtca
    15361 gattcattcg gatagctaat ggacgtagtg ttgcttctag ttttgataac tgttctttaa
    15421 cggatatatt ttctcttatg tcattctttg tctgaatcaa tacttttcgt tcccaatctc
    15481 gtgtattcgg attcaaaatc agattataaa gtgtttgaat gaatatttgt tcctcatcca
    15541 tagattctcc tcctttccga agaagacaag ctagtgactt tagctgtaat ttatcataaa
    15601 gtgaggaaat aagcgattac ttcatccccc tacagatttt tcgtataaaa aagagagaat
    15661 ccttatcgac agattctcct ttttaatcaa tttattttat aaatgaaaag ttaatttacc
    15721 ttttatggaa aactatttta aaaaataaac ataaatatgc agttaatgta acaagataag
    15781 aaataatagt cgttactgta taacttgtgc ttactttaat atcagcaact cctttgactc
    15841 cgcttatttg taccaatcca ttagcattca cctgctcctt tacaggttga tgatttattt
    15901 ctacattcgt tcctaaataa gcatatacag gaagtgtgat
//
LOCUS       DACDEG010000054        15775 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.056, whole genome shotgun sequence.
ACCESSION   DACDEG010000054 DACDEG010000000
VERSION     DACDEG010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.056"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(9..311)
                     /locus_tag="IV142_001773"
     CDS             complement(9..311)
                     /locus_tag="IV142_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485433.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(531..897)
                     /gene="ssrA"
                     /locus_tag="IV142_001774"
     tmRNA           complement(531..897)
                     /gene="ssrA"
                     /locus_tag="IV142_001774"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1068..1841)
                     /locus_tag="IV142_001775"
     CDS             complement(1068..1841)
                     /locus_tag="IV142_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8485434.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1985..2335
                     /locus_tag="IV142_001776"
     CDS             1985..2335
                     /locus_tag="IV142_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP8485435.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2316..3032
                     /locus_tag="IV142_001777"
     CDS             2316..3032
                     /locus_tag="IV142_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8485436.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3032..4480
                     /locus_tag="IV142_001778"
     CDS             3032..4480
                     /locus_tag="IV142_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8485437.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4550..5059)
                     /locus_tag="IV142_001779"
     CDS             complement(4550..5059)
                     /locus_tag="IV142_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485438.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5049..5774)
                     /locus_tag="IV142_001780"
     CDS             complement(5049..5774)
                     /locus_tag="IV142_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485439.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(5997..8117)
                     /locus_tag="IV142_001781"
     CDS             complement(5997..8117)
                     /locus_tag="IV142_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8485440.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8347..9525
                     /locus_tag="IV142_001782"
     CDS             8347..9525
                     /locus_tag="IV142_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP8485441.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9541..10299
                     /gene="aroD"
                     /locus_tag="IV142_001783"
     CDS             9541..10299
                     /gene="aroD"
                     /locus_tag="IV142_001783"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP8485442.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10322..10807
                     /gene="ybaK"
                     /locus_tag="IV142_001784"
     CDS             10322..10807
                     /gene="ybaK"
                     /locus_tag="IV142_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP8485443.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10878..12131)
                     /locus_tag="IV142_001785"
     CDS             complement(10878..12131)
                     /locus_tag="IV142_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8485444.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12248..13597
                     /gene="gdhA"
                     /locus_tag="IV142_001786"
     CDS             12248..13597
                     /gene="gdhA"
                     /locus_tag="IV142_001786"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP8485445.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13709..15055
                     /locus_tag="IV142_001787"
     CDS             13709..15055
                     /locus_tag="IV142_001787"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP8485446.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(15363..15749)
                     /locus_tag="IV142_001788"
     CDS             complement(15363..15749)
                     /locus_tag="IV142_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8485447.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         5161 a         2822 c         3170 g         4622 t
ORIGIN      
        1 agcctacttt aatctttata cctttctgga tcaacgaaag tatacttcaa atagtcataa
       61 cgccgatgat cgctccgtgc gtctggcaca tcagtcacga tatcaaacaa aaaatacaca
      121 tctttcttca ttctggtttt tgttgccgga attttaaagt agttcttatt agagtaatag
      181 agattgatta ataagctatc ttcgattgct aaaaagaaaa cttctgaatc ccacacctta
      241 taaaaatctt tgacaaatct attcgaagga tcaaatttaa accataattg tgtttttcct
      301 tccatcaaca taatgttcac ctcaaaaaga gtatacgaac taatgttctt tttgtaaagt
      361 aggaatatca ttgataatat ggatggaaca tatatattaa aaagtgttct ttttgaatgc
      421 tattttgaat gccattttca ttcatttaac gaaaatctgc gaaaataaaa aatgctcaaa
      481 cccttataaa ataaggattt aagcatctag cgaaatctgc tgaaatctca caatggagac
      541 ggcgggagtc gaacccgcgt ccaaacacat cgccacttaa aaatctacgt tcatagtcaa
      601 ctcatttaag gattcacttt acagcttgcc gagtgacagg cattctgtat cgctagtctg
      661 atgttctctt tttcaactta cagacggaaa gttgaaacgt atcccactaa agttaggacc
      721 cttactcgag cacatgggcg atgccgagag gatctacgct aactgttttt aggcagctaa
      781 agcgtaagag ttgttttcgt ttttagcagt tatatttaac tgtaacgttt ttacgtagac
      841 gtaacctacg aaacgcaatt caagctcgac tgtgcctgtc gaatccgtaa cgtccccgtt
      901 agctaacaag ggttacgtgt atttttcata caaaattagt atagcatata ttgggataaa
      961 ttcaataaaa aagcgaatca tatcccatta tttatataat aactgcccct ttcttaagaa
     1021 aaaataagcg attttcttaa gaaaagaggt agcttcaaaa aaagatctta aatcagatca
     1081 aaatacttca atgcgtggac aattccgcct tctgtatttt tctttgtgat aaaggtagaa
     1141 agttctttta atccgtcata ggcgttgccc atagcgattt tgttgtcgca agcttccaac
     1201 aaggcaaaat cattcggacc atcgccaaat ccaaaagtag gaactttttc cagctgaagt
     1261 tctttttgca gacgtttgac acctgcccct tttgagattc ctttcttcac tgtatcgatc
     1321 gagtaaggac cattccgata aaaagttagc tcaggaaaat gttcgtgata gtattcgtca
     1381 cctgattgag ataagatcag caacatattt actgtgtttt ctttaaagcc taaaggatca
     1441 atttccggaa catctgaatg aatgaagtcg taagcattga tcattgtttg gctatgaccg
     1501 gtacaccaga ttttctgatc gttatagtaa gaaagttcat gaccgttcaa ctgtacatga
     1561 tcatacattt tttgacattc ttctgaagaa aatgtttgag aatagatttc ttcgccgtct
     1621 actcgaatga aagaaccatt catggtgacc gcagaagtaa tgcccgcatc ttccatgata
     1681 gagtggattt ctgcttctgt acgtcctgta gcgatgacgg ggagaacatt gttttctttt
     1741 aatgcgttca tagcttttgc cacttcgggt gtgatttttg atttcgcatc tagtaatgtt
     1801 ccatcaagat caaagaaagt gattgcacga taatttgaca caaataaaac tcctttttta
     1861 ttggaataag tttatcttaa caaagaatga acaaaagaca aacctttcgt gaagaattcc
     1921 tgaacatatt ggttcagtca acaatttaga gtatactata agaagagaat ttaggaggga
     1981 aagaatgatt tttttcagta ttggtatctt attcttaata attggcagtc tttttcggat
     2041 tctgccctca aaaggaaatc tgccttttta tgggtatcat tcgcctcttg ccgcaaaaac
     2101 agacgctcat tggcggctgg ctcaaaagac aagcggaaac tggttttttc ttatgggctt
     2161 actgatggca ttgatcggct attatttaaa gacatctggg catacgaatt attttctgat
     2221 tgaaatgctt ctgctagtat ttccaattat gcctattttt attatgacag aaaagaaatt
     2281 acagaaatat gacctggaaa ctggagggaa cgataatgaa tattttaatg attgaagata
     2341 atcaatctgt ttctgaaatg atgcagatgt ttttcttaaa tgaaggttgg gaagcaactt
     2401 tcaagtatga tggaaaagaa gggctcgatg cctttttaga aaacccacaa cattgggata
     2461 tgattacact agacctgaat ttgcccacac tggacggtat gctagtagga agagagatcc
     2521 gcaaagtttc aagtactgtg ccgattatca tgctgacagc tagagattct gaaagcgatc
     2581 aggtgatcgg cttagagatg ggggcagatg attatgtcac aaaacctttc agtcctctga
     2641 cgcttattgc tcgaatgaaa gctttgcatc gccgtagtga gttggcagaa gcaagagaag
     2701 aagcagtagt ttcagatgat cattttgata tcgagactag tcatttcaag atgaatacga
     2761 aaacacgaga agcgtatctg gacaaccggc caattgaagg actgacgcct aaagagttcg
     2821 atctattgtt tactttagca aaaaaaccac gtcaagtatt ctctcgggag cagttgcttg
     2881 aattagtctg ggattatcag tactttggtg atgaacggac agtggacgcc catatcaaga
     2941 aattacgaca aaaaatcgaa aaggtcggac cacaagtcat ccaaactgtt tggggagtcg
     3001 gctacaaatt cgatgattct ggtgttgcct aatgcgttat ttgtatcagc aactcttagc
     3061 tttctgcgga atggtcgcgt tgatcttatt gatcgttgga gtttctttta cacaactgac
     3121 aaaacaaacg attgaagaaa acaattacca gcaactgttt ggatatgctg aatcagttga
     3181 aaagacaacg caaacctatg ccgatacgct cccgcagttt agtcaagatc aggcgtttca
     3241 aaatgcattg ctgctgacag agcaagtact gacagaacaa aatgtgaact ttatatttat
     3301 cgataaaaac gaacgtgtca tttatccgac taatacagct ttactgaatt ttaccttaac
     3361 agctgatcaa tggaaaaatc tgaggaatgg acgtcaagaa aaattcacat cgaataaaaa
     3421 tattttagga caagcacagg cgacctctta cgcacttgtg ccatttaatt tgaatcatga
     3481 attctatgga gcacttgtag ttagtcaacc tgcgcgaaat atcgataaca gcgttcgtcc
     3541 aattacactg aacctgttca aaggatttat tttttccagt atcgttgcag ttattgccag
     3601 ctatgctttt gcagcattcc aagttaagcg tatcaatcgg ttaaggaatg cgacgaaaga
     3661 agtgacgaat ggtaattttg atgtacagct ccccgtacat gacaaagatg aatttgatga
     3721 gttagcagat gatttcaata aaatgacgaa ttcattgaaa gaatctcaag cagaaattga
     3781 agaacaagaa aatcgtcgaa gacaatttat ggcagatgct tctcacgaga tgagaacacc
     3841 tcttacgaca atcaacggac ttctggaagg gttagaatac aatgcaatcc ctgaaaatca
     3901 gcgagaaaac gcgattaaat tgatgaaaaa tgaaacagag cgtctgatcc gtttggtgaa
     3961 tgaaaacttg gattatgaaa aaatccgtac gaatcaaatc agtatggtca ttaaaaaatt
     4021 caatggaaca gaaacattga aaaatattgt tgcacagtta gaagctaaag cagaagcagc
     4081 aggagataca ttgacattga aagccgttga tgatattgat gtttacgctg attatgaccg
     4141 atttgtccaa atcatggtca atattattca aaacgcgata cagtttactg aaaacggcca
     4201 aatcacgatt actttagaaa aaggctatct tgaaacgatc atcacgattg aagatacggg
     4261 aattggaatg tctgaacaac aaatgaaaag tatctgggat cgttattata aagtagatcc
     4321 ttcaagaaaa aatacaaaat atggtgaatc aggattaggt ttaccaatcg tgcagcaact
     4381 tgtacgcttg cataaaggaa aattagaagt agaaagcgaa ctaggaaaag gaacaaaatt
     4441 cgctgttcgt ttgcctgacg tagaaatcaa aaaagagtaa aaaacaagcg gtataaaaga
     4501 tcatgaatcg atcttctata ccgcttgttt ttactataaa atgattgatt tagcccgcgg
     4561 gctctaaacg atcaatataa ggactttgag caatagtagc atcatgagta aagagtaaga
     4621 taccttgatt ttcttcatga gctaaataag aaagcaagtg gagtagctct tggatctcca
     4681 atacactaag ttcatcaaat gtatcttcaa taacaatatt gtttcgcttg gaaagcaagg
     4741 catgaagtaa ttgcagcttt actttttcaa aagacgtcaa ctcagctgcc gcacaattaa
     4801 gcaaggaagg agagatatca agtttttctt gataggccaa acgatcttgt ttgatttctt
     4861 tttccggtac atccaacagg acatggtctt taagattcag ataaggaaac atcggccaat
     4921 ttttttggat gatcgcaaaa ttatccagtt ttgcaacaag aacctctttt ttcaagtgct
     4981 ccaatatttt atgctgtttt tcgagaggac acaaaataac ggtcaagtgt tcagcatgaa
     5041 tcaaatattc aggtttcatt acgtccaggc ctcctacttt gcaaatgatg gataaaaaaa
     5101 gtgaaaggga atagactaag aatgagacag catccactga gcccaataag agtttgggca
     5161 aatcccttaa gtatatctgt gaaaaaaaga tcattttgga aatcgatggt aaaaaaagtc
     5221 tgttgatcca tcggaattgt aagtccgctt accgaagtca agatatcacg gatatctggc
     5281 agttcagtag tctttaagac aagaaaattg atcctttgaa gaagcgcttc ataaactgac
     5341 tggaagctta cgatccacaa taaaaccaag atacaacaaa gaaagagagg aaagaacagc
     5401 tgccagaaaa taaaaaggaa taatttggtt ttagaaagac cagtcaggtg ccaattgatc
     5461 aattcctgct tgttttgcct gactgaatag taaaagaaaa agataaaaag gaaggcaaat
     5521 agaaaaaata aactgaatat gccttttata taaaatcggc tagctaattc taaagccgga
     5581 agaagctctg tagatgcaga agcgccaata aatgaaagcc gatcttggac ttgattggct
     5641 aaagccgatt gcaaatggta aagagtcagg catgctatca ataaaaaagc agaaaagctt
     5701 gaaaataaac aatagagtat tgtctgcttt ttgtaataaa ttaatcgagc agtagcatag
     5761 cggattattt tcatactttt cacctctata aaaagtatag aaaatgaata tgaataaagt
     5821 atgaaattcc tttagtgaaa tcacgaatta aaaccagaaa atattttgta aaaagcaaga
     5881 ggttgtgaag aagctgccct attctattac cgtagatgca agcttttgaa cactgtttat
     5941 tcgaaaagag gagcagcgac aataattttg tcgctgctcc ttttaaattt attttatcat
     6001 tcggaagaac ttgtggtcgt tccgtgaagt tcttggtacg tttgcgtttt ataaagatca
     6061 acagttgatt gtccgttgtg ttcactaaag acactagttg acttgtcacc aagagatttt
     6121 tcagcttctt ttaatttctc taagccattc ttgtagtcat aatcactggt atcaattggg
     6181 tctaatccac tattcgtata gaaccgaaga agatcaccat tattgatttg gtcagaaaca
     6241 gctaactgtt tatcaacttt ttctttcagt gaatcaattt cattctgtac ttcctcagta
     6301 ggttcagtaa tagcttcacc agtttcgtta ctatagatcg taccaccgta gtaagtatat
     6361 tttggtgtaa caaaatcacc atcacggaaa gaaacaagct gttcatgatc tttcgagaat
     6421 agatcttgtc ctaactgaat ataatttttt gtatctacac ctagtaagtg aagtaatgta
     6481 ggcaaggcat caacttgtcc gccgtaagtg tgattgattc cgccctgtgt ttgtccaggg
     6541 atatggatca tataagggac acgctgcatt tgtgcattgt cgtagtttgt ccaagtagaa
     6601 cttgtttttc caactaattc agctagattt ttatttcttg aattcgagac accgtaatgg
     6661 tcaccgtaaa ggacgatgac tgaattatca tacaagccgc tttctttcaa ataattgaag
     6721 aattcttgta cggcggtatc taaataattg gctgtcgcaa agtaaccgtt gatcgtttca
     6781 tcatttgtat tggctaacgg gaatcctgct tcgtcattgg tgaattttga ataaggataa
     6841 tggttagata cagcaatgaa ttttgaataa aatggttgtt gcagacgttc caagtattgt
     6901 acggattgtt ggaaaaatgg tttatcatgc aacccgtatt ggaacgaatt gtcatcatta
     6961 acatcataat aagaagaatc aaagaaataa tcatatccta aattcttgta tgtctcgttt
     7021 cgattccaga atgtaccagc gttcccatgg aataccgcac taatgtaacc ttgtgtttga
     7081 cttaaaatat caggtgcagc ttggaaagta ttttttccgc ctaattgtgt gaacaaggag
     7141 ccttgattca agccaaacaa tgaattttcc atcagcgttt ccgcatcgct tgtttttcct
     7201 gcttttactt gatggaaaaa gttgtcaaaa ctgaacgtgc tgttgctgtg gtaaagacta
     7261 ttgatgaaag gtgtaacttc atgctggaga ccattctcat ctgtcaattt ataatcgatc
     7321 aggaattgct ggaagctttc taaatggata taaatcacat tttttcctct agcgagcccg
     7381 aacaaatcgt catttggtgc tgcataatgt tcttttacat aattttctac ttctttcata
     7441 tcattgggac ttgcttctgc ccgcacttgg tttgtctggt aagtttgaac accgtcataa
     7501 accatgaaag cattcaagcc aagatatttg accaaataat cacgagagaa tgttcgagtc
     7561 agcaattctg gacgatcagc ttccgccata aaaagatttc ctgagaaaat cataactgct
     7621 aatgtagaga tcgcaaaagc cattcttgct cggatcgggc gagggtccaa cttgattttt
     7681 tttgcaatca gcagaacagt caagataata aaatcgatcc aatagatcac atcatatggg
     7741 cgaaacagtc gaatggcact ttctcccaga ccactcgcta ctttcccagc acctaaaatc
     7801 gtgttgatcg taatgaagtc cgtgaattct cgataataag tgacattcga aaaaacaagc
     7861 aatgtcaaaa gaaaataaat gaccatcatc gtgatataag caactctagg gcgtctgata
     7921 taaagagcaa ttccaagtaa taacaatgta gttgcaatag ggttgataaa caagataaag
     7981 tattgaatgc cactttcgat tccgagatgg aaatcaacta aataggcaaa aatcgttttc
     8041 aaccaaagta aaacgactag taaagaaaaa aatccgagcc tcttattcaa aaaggcaggt
     8101 gtttttatac atttcaagat aatcttcctt tctgcacttc ttaatcacag aagtgtcctg
     8161 aaaccatgtt acacagctca cttattctca tttattttac tagtatgaca atatagtgtc
     8221 aattgaaacc agaagtttat gctgtttcat taaggttttc tttaagcttc atgaaaaaat
     8281 gagcagtaag acaagtccta tgttttttgt tatacttggg aagagaaagc taaagcaggt
     8341 gaaggaatga aagtaaaagt aaaaaaaaga gcaagcaatc gattgaaaaa aggatatcca
     8401 ctgatccaaa aagaagacct tgctcatccc caaaaagaaa ccacgcaatg ggtggaatta
     8461 gttgattcgc aaaaccaatt tatcgcaaaa ggatatctag gaaagcagaa taagggaatc
     8521 ggttgggtat tgagccagca gaacatccca tttgatcaat ctttttttga agaatttttt
     8581 gtgaagtcaa aagaaaaaag aactttcttt ttttcagatg attctactac cgcttttcgt
     8641 atattcaatg gagaaggaga cgggatcggc ggtctgattg ttgattatta tgcaggttat
     8701 gctgtttttt cttggtataa cgaaacgtta tatgcacatc gtgaattatt gattcaagct
     8761 tttcgtgaaa tctacccgga aatcatagga ggctatgaga aaattcgatt tgaatcagta
     8821 aatctgcctg agagccagtt catttacgga aaagaggcac cggaaccctt gatcgtaaaa
     8881 gaaaacggag taaaatttgc cgtttattta aatgaaggat taatgactgg gatcttttta
     8941 gaccaaaaag aagtacgggg atcattagca gaaggtttcg cttctgggaa aacagttttg
     9001 aacatgttta gttataccgg cgccttttct gttgctgccg caatgggagg ggcaatcgaa
     9061 acaacaagtg tcgatctagc aaaacgcagt ttaaaaaaaa cacaggaaca gtttgaagtg
     9121 aatggtttgg atgtcgatca gcaaaaaatc attgttatgg acgtattcga gtatttcaaa
     9181 tacgccaaaa gaaaacaatt tttatatgac atgattgtat tggatccacc aagttttgcc
     9241 agaaataaga aaaaagtctt tcgcgtagca aaagattatg gggaactggt caaggatagt
     9301 ctggatattt tagcagaaga cgggatactt attgcgtcta caaatgctgc gaatgtctcg
     9361 atagaacaat tccaaacaat gattgaagag gaattccatg ccaaaaatgt ttcttttgaa
     9421 caacttgctt tatatcgtct gccaaaagat ttccagacga cggatacctt tttagaaggc
     9481 aattatctga aagtgtttgt ttatcaaatt aaaaataacc attagaagga gcgatgcaga
     9541 atggttcggg taaagggatt gaatatagga agcggtagac caaagatttg tgcgcctctt
     9601 atcggggccg tccgagagga agtattacgt gaagcagttc ttgcacgtga agcaggggcc
     9661 gatttagtcg aatggcgagt cgatcattat cgtgaagttt tttcttttga tgaagtagca
     9721 gaaacattgg ttcttatcca tgagatgctt gatggactgc cgttattatt tacgtttcgt
     9781 accttagctg aaggcggaga acaagacata tcgatccgtc agtatcgcga cctttatgaa
     9841 catgtgatac acacaggatt agtagatatg gtcgatatcg agttgttcaa agtagaaagt
     9901 ctaggaaaaa gactgataga ggaaatcaag tctttaaaca ttcctttaat tatcagtagt
     9961 catgatttta aagaaacacc agcagaccct gtcttgcttt accgattgaa tgttatggaa
    10021 cattttggag ccgatatcgg aaaactggca gtaactccca ataatgaacg agatgttttg
    10081 cggttgatgg agttgactag acgggcaaat gcttttgtct ccatgcctat tattacgatg
    10141 tccatgggaa atcttggtaa aatttctcgt ctggttggcg gaacgacggg ttccgttatg
    10201 acatttggtt ccttaagcag cgattctact tctgctccag gacaattacc ggtcaaagca
    10261 ttgaagcaaa ttattcaatt attaaatgaa ccgttgtaga acaagtttga ggtgatattt
    10321 tttggcaaag aaaaaaatag taaaaacgaa tgcaatccgt atggtcgaac aaaaaaagat
    10381 tccctataca gagcacgaat atgagtggga cgaaagccac ctaagtgcgt catcggtcgc
    10441 tgaacagctt ccagaaagcc aatctcgtat atttaaaaca ctggttgctg tgggcaatgt
    10501 gacggggcct cttgttgctg tgataccggg agaagccgaa ttgaatctaa aaaaattagc
    10561 aaaagtttca gggaataaaa aagtagaaat gctccattta aaagatttgg aagcaacgac
    10621 cggatacata cgtggtggct gctcgcctat aggtatgaaa aaactatttc ctacttattt
    10681 agatcaaatt gcagaaagct atgaacaaat cattgtctcg gctggcagaa gagggctaca
    10741 gatggagtta gcacctcaag atatctgtgc attaacgagc ggacaatttg cagatataaa
    10801 acaataaatc atacgtataa aagaaagaag gaggccggga tgttgtttgt ccagtctcct
    10861 tctttctttt ttatacatta tgctctttgc ccggcattca ttcctgctac atgccctgta
    10921 gcgaaagccg ctgtgatatt aaagccgcca gtataaccat tgacatccag taattctccc
    10981 gcaaaataca gaccatcaat gattttactc tccaatgttt taggataaat ttcctttaga
    11041 gaaacccctc cgccagttac aaatgatttc tcaataggaa aagttttgtt gattgatagc
    11101 ttaaactctt tgcatagttg aacaaaatct tgaatttgtt tttgagaggc ttgttgtcct
    11161 gtcagatgat ccatttcaag ccgctcaaga aagaaaacta acaatcgctc aggcaatata
    11221 ccatgccaag cattgaccaa gttctttttg gttgctttac ttttttctgt caatatatga
    11281 ataagctctt gtttggtttg tgtagggaaa caatccagag aaagtgtcac tggttctccc
    11341 gttttctcta attctttatt gacaaaactt gaacaacgca gtgcagccgg accagaaata
    11401 ccgaaatggg tgaaaagtaa atccatcgta tgttcagtca atacacgacc ttttttattt
    11461 aaaacgcgta aagtgatgtc ttgtaaagaa agcccttgaa gtgttttctc ctgaatatac
    11521 ggttcatccg atatcaaggg ggactctgtc gcataaagag gcgtcaccgt gtgacccact
    11581 cgcttcacga tcttgtaccc atctccagta gaaccagtag atggataagt ccgtccacca
    11641 gttgttaaga taatggcagg agcatggaac tcttcgaatt ccgtgcgtat accgtaaatt
    11701 ttatgttctt tgtggatcaa tttctctact cgagtagaga atagaagcgt cacatctagt
    11761 tctttcagac gattgagcaa tgcttcgata atcgtttttg atttatttgt tacaggaaac
    11821 atccgtccat gatcctcttc ttttaaatgt acgccttgtg attcaaagaa gttcatgata
    11881 tcgaaattat tccattgcgc aaacgtacta tataaaaact ttccatttcc aggaatatgt
    11941 gcaatcaaat catcaacagg acgattgttc gtaacattgc accgaccacc acctgtcatc
    12001 agtagttttt ttcctgcttt tttgtttttt tcgatcaaga gtacgcgtgc gccttgttca
    12061 gctgcactga tcgctgccat catcccgctt gttccagcgc cgactacgat cacatcatac
    12121 agtttttcca ttccctcacc tcaaggaaag tgtagcatat ctgaagaaag aaaacagaaa
    12181 aaaatgtttt gagccaaaag agaaaatgag ctaaaatgag ggaagataag caaaggagcg
    12241 tggaacgatg acaaacgcaa cagaatatgt tcaagcaatt caagaaaagc tacatcaaaa
    12301 ggatcaaggg caagaagaat ttttacaagc aatcgatgaa ttcatgccca cggttatgtc
    12361 ttttttggac actcatcccg aatatatcga aaagaatatc ttagaacttt taacggaacc
    12421 agaaagagtt attcaatttc gagtgccttg gcaagatgat gaaggaaact ggcgagtaaa
    12481 tagagggtac cgtatccaat acaactcagc tatcggccct tataaaggtg gattgcgctt
    12541 ccatcctagt gtcaatctaa gtatcttgaa atttttggct tttgaacaga ttttcaagaa
    12601 tagtttaacc ggtctgccaa tcggtggagg aaaaggcgga agtgacttcg atcctaaagg
    12661 aaaatctgat aatgagatca tgcgtttttg tcaaagtttc atgctggaat tgtcaaaaca
    12721 tatcggccca tcgattgatg ttcctgcggg agatatcggt gtaggagcta gagaaatcgg
    12781 ctatttgttc ggcgaatata aacgattgaa aaaatatgat actggtgtgt tgacaggtaa
    12841 acctttggat ttttggggaa gtaagatacg tacggaagct actggttatg gattagtgta
    12901 ttatgtcaaa catctattga acgaagaaaa agattcattt aatcaaaaaa cagtttttgt
    12961 ttccggaagc gggaatgtag ccatttatgc aatcgaaaaa gtgcaagagc taggcgggaa
    13021 agttgttaca tgttcagatt ctagcgggta catctatgat ccagaaggaa tagatgtgaa
    13081 actattgaaa gaagtcaaag aaatcaatag acaacgtttg acggaatacg caaaccaacg
    13141 tccttctgct atttatcatg ctggagaatc tgtttggaca ttgaaagaaa aagcggatat
    13201 cgctttgcca tgtgctactc aaaatgaaat agacgaaagc ttggccgaaa tcttagtaga
    13261 aaatgggatc aaaatcgtag cagaaggcgc taatatgcca tgtacagtaa aagctgtttc
    13321 tgttcttcat gaagcaggcg tgtggtattg tccaggaaaa gctgctaatg ctggaggagt
    13381 agcagtatct gctttagaaa tgagtcagaa cgtccagcga ttgacttggg aaagagagca
    13441 agtggatgag cagctggatc aaatcatgga aacgatttac catacgtgtc gccaaacagc
    13501 agccgagtac ggcgacgaaa aaaatctgct tcttggagca aatgttgctg gatttgaaaa
    13561 agttgccaaa gcaatggcag cagaaggttt agtttgaaaa atgaaacttt cttggaaata
    13621 ttgtcagcat tttgtttatt tgcttgaaaa ataataaaaa aaaacgtaaa gtattacctg
    13681 tagagtaaac ctataaggag gagaacaaat ggcacacatt cattttgact attcaaaagt
    13741 agcaccgttt gtcaacgaac aagagttagg gtacatgcaa agccaagtaa cagcagccca
    13801 caacgaatta cgggaaggta ctggcgcagg aagcgacttc cgcggctgga tcgatttacc
    13861 aacgaactat gacaaagaag aatttgcacg tatcaaagaa gcagctaaaa aaatccaatc
    13921 tgattctgaa gtattagttg ttatcggtat cggtggttct tatttaggcg ctcgtgcagc
    13981 gatcgacttc ttgaatcaca cattcttcaa tttattagat agcaacacaa gaaaagcacc
    14041 acaaatcttc tttgccggaa attcaatcag ctcaacatat attgctgact tgatcgaagt
    14101 aatcggtgat cgcgactttt cagtaaacgt tatttcaaaa tctggaacaa ctactgaacc
    14161 agcgattgcg ttccgtgtat tcaaagagct tcttgttaag aaatatggac aagaagaagc
    14221 aaacaaacgt atctacgcta caacagataa agcaaaaggt gcagttaaag tagaagcaga
    14281 tgcagaaggt tgggaaactt tcgtgattcc tgatgacatc ggcggacgtt tctctgtatt
    14341 aactgctgtt ggtttgttac caatcgctgt tagtggtgca gatatcgatg cattgatgca
    14401 aggtgccgct gatgcaagca aagcatactc aagcgacaaa ctagaagaaa acgaagctta
    14461 ccaatacgca gcaatgcgca acattcttta ccgtaaaggc aaagtaactg agttgttgat
    14521 caactatgaa ccaggaatgc aatacttctc agaatggtgg aaacaattgt atggtgaatc
    14581 tgaaggaaaa gaccaaaaag gcatctatcc ttcaagcgca aacttctcaa ctgatttgca
    14641 ctctttagga caattcatcc aagaaggacg tcgcaatatt tttgaaacag ttgtgaaggt
    14701 tgaaaaacct cgtaaatcaa tcacaattcc tgaacaagca gaagatttag acggtctagg
    14761 ctacttgcaa ggaaaagaag tggacttcgt aaatacaaaa gcatttgaag gaacattgct
    14821 tgctcataca gacggtgacg tgcctaactt gctagtcaaa attccagaaa tggatgctta
    14881 tacattgggt tatacaatgt acttctttga aattgctgta ggtatttcag gttacttgaa
    14941 tggaatcaac ccattcgacc aaccaggtgt agaagcttac aagaaaaaca tgttcgcact
    15001 tctaggcaaa ccaggctttg aagatcttgc taaagaattg aacgaacgcc tgtaagataa
    15061 gggtttttcg ctagatttaa ataatcagtg atagaaacat gttcgcttta aaatgaaaac
    15121 tggtacccat ttaggtactc aaacatactg catcgtttat tcagaataag gggctgtgac
    15181 aagacgtttg tcacagtccc ttgtttgtat agaaatcgat tgattgcctt gctctttagt
    15241 ttgttttagg actttaagaa cagtgaggct gacttcgaag gttaattctg tttttttgtt
    15301 ttctagaatc agtaagcaaa aaagcactca cttcgagtgc ttttttgcga tatattctta
    15361 gcttaagata ggcctaattt ttcttttaca gatgagggta ccttatcgct ggtaattact
    15421 tttttctcta tgatccgctt ttcgcttgct ttcaactgca agatcgttcc ttcggggtaa
    15481 gcagaaccat tggcagagtc accagaagta atgacatcga aatctaactg tcttttttca
    15541 ccgttttcag cataagcatc tacgttgtat ttaaaacctc ttccaattac ttccccatta
    15601 tctgctttga tatcagtttc tgcaggcatt ggagctttga ctaccgcata ataatcttta
    15661 cctacataag tagactgata gtagtcccaa cctttccatc ctataaaagc aatcaatatg
    15721 ataacgatca accctaaaat ttttttcatg aaagatctcc ttttttattt ttatt
//
LOCUS       DACDEG010000055        15669 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.057, whole genome shotgun sequence.
ACCESSION   DACDEG010000055 DACDEG010000000
VERSION     DACDEG010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.057"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IV142_001789"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IV142_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP8485448.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IV142_001790"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IV142_001790"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP8485449.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="IV142_001791"
     CDS             complement(2169..2906)
                     /locus_tag="IV142_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP8485450.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="IV142_001792"
     CDS             complement(2997..3524)
                     /locus_tag="IV142_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP8485451.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="IV142_001793"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="IV142_001793"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP8485452.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="IV142_001794"
     CDS             complement(4401..4886)
                     /locus_tag="IV142_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP8485453.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="IV142_001795"
     CDS             complement(5039..5236)
                     /locus_tag="IV142_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485454.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IV142_001796"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IV142_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP8485455.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IV142_001797"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IV142_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP8485456.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="IV142_001798"
     CDS             9211..9423
                     /locus_tag="IV142_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP8485457.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="IV142_001799"
     CDS             9425..11104
                     /locus_tag="IV142_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP8485458.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="IV142_001800"
     CDS             complement(11358..11558)
                     /locus_tag="IV142_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8485459.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..11869)
                     /locus_tag="IV142_001801"
                     /pseudo
     CDS             complement(11708..11869)
                     /locus_tag="IV142_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            complement(12249..12322)
                     /locus_tag="IV142_001802"
     tRNA            complement(12249..12322)
                     /locus_tag="IV142_001802"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12286..12288),aa:Ile,seq:cat)
     gene            complement(12634..13032)
                     /locus_tag="IV142_001803"
     CDS             complement(12634..13032)
                     /locus_tag="IV142_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485460.1"
                     /translation="MDKGNSVTFALENISLNKIHFELTDNFSYGDLEFNIELGYKEDE
                     AEYLEKKLVSLRAHIVGGMEGAPVDILLFSVIRVSTNEQPYKKLVEESMGELAKPILN
                     KASNLISNLYSEVGVMPTVIDLYQLYSQQK"
     gene            complement(13025..13477)
                     /locus_tag="IV142_001804"
     CDS             complement(13025..13477)
                     /locus_tag="IV142_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485461.1"
                     /translation="MKSINDLLKKHPDLYKKLNSEQRNLSKQLLKIRLKKQKNVTEFS
                     KMLGISEKKYLEFEFGDMHIPVAEYNKLLKQVENLSYKDSMNFTIKVAGGPIKYDRIE
                     NKPYIRNKSYSVFKSRKRKEVSSNMVYASVNYQDTSRYIVEKEVYVHG"
     gene            complement(13464..13871)
                     /locus_tag="IV142_001805"
     CDS             complement(13464..13871)
                     /locus_tag="IV142_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485462.1"
                     /translation="MFFLPLKYLEIYSVKKNKLIGENDSPIKTPIELSLRNRMGILQR
                     SEIVKELFYHKFSYEIRLDDTYEHYRIVFFVYNSIEDATATTALTFGFTKNGTINSDK
                     TRQAATESDDICKKVCNGEENCWIGEEKLNEIY"
     gene            13850..14152
                     /locus_tag="IV142_001806"
     CDS             13850..14152
                     /locus_tag="IV142_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485463.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(14188..14559)
                     /locus_tag="IV142_001807"
     CDS             complement(14188..14559)
                     /locus_tag="IV142_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485464.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(14561..14962)
                     /locus_tag="IV142_001808"
     CDS             complement(14561..14962)
                     /locus_tag="IV142_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485465.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            complement(14976..15383)
                     /locus_tag="IV142_001809"
     CDS             complement(14976..15383)
                     /locus_tag="IV142_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485466.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
BASE COUNT         4668 a         3227 c         2576 g         5198 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa taacattacc agaaggataa tataggttag
    11881 tacacgtata aaaatatctt ttatattgac acaatttatt taacaagtat aataataatt
    11941 gaaagtgaat ttaaacaaat atacaccata aacacaacgt acctgcgaat acgttgtgtt
    12001 tttttctttt tcaaaaaaag acgcttaccg ctgcgaaaca gtaagcgcca ttgtcggctc
    12061 taatgtttat ctagccaatg actaaataat gccaaagtta aaccaattaa caatggcaag
    12121 agcacgtgct ccaaagtgaa ttcaaacaaa tcatacacct cctcccacga aaatctctgt
    12181 ggaagcgacg agccgatatt ttattatacc attattgaat tttatttttt atcaaataga
    12241 ttaaattatg taccctgtag gactcgaacc tacgaccgga cggttatgag ccgtctgctc
    12301 tgaccaacta agctaagggt actggttgtt gccacataaa gccataaaca atcaaccagt
    12361 agaatgtgtg gcaacaaacc tgttatcgca tagcttggag tgtgactatt atgggtgata
    12421 gtgaagatat gcgataacat cactatttta tcgaatgatt tttatagttg tcaatacagt
    12481 catctaaata cttcatatca atcatttatt cagaaacaaa ctatcttgca ggatctatac
    12541 gagttaatta cagagcaaaa aagcctcaac caaggatgac tggttgaggt ctttgtcttg
    12601 caaatttaaa tagataactc tattacttgt attttatttt tgttgactat atagctgata
    12661 taaatcaata actgtaggca taactcctac ttcgctatat aaattagaaa tcaaattaga
    12721 agctttattc aggataggtt tagctagctc tcccatagat tcttctacta attttttata
    12781 tggctgttca tttgtagaaa cacgaataac tgaaaagagt aaaatatcta ccggtgctcc
    12841 ttccatcccc cctacaatgt gagctcgtaa agaaacaagt tttttttcta gatactctgc
    12901 ttcgtcttct ttatatccaa gttcaatatt aaactctaaa tctccataac taaaattatc
    12961 agttaattca aaatgaattt tattcaaact aatattttca agtgcaaaag taacactatt
    13021 tcctttatcc atgtacatat acctcttttt ctacaatata gcggctcgta tcttggtaat
    13081 taacactagc atagaccata tttgacgata cttcttttct ctttctactt ttaaatactg
    13141 agtaactttt attccgaata taaggtttat tttcaatacg gtcatattta atagggccgc
    13201 cagcaacctt aatagtgaaa ttcattgaat ctttatagga caaattttca acttgtttca
    13261 aaagtttatt gtactctgca acaggaatat gcatatctcc gaactcaaat tcaagatatt
    13321 ttttttcact tattcctaac atttttgaga attctgtcac gtttttttgt ttttttaatc
    13381 ttattttaag taattgtttg gacaggtttc tttgttcaga atttaatttt ttatataaat
    13441 caggatgttt ttttaataaa tcattaatag atttcattca acttttcctc ccctatccag
    13501 caattctctt ccccattgca tactttctta catatatcat cactttctgt agcagcttga
    13561 cgggttttat ccgaattgat tgttccattt ttcgtaaaac cgaatgttaa tgcagtagtc
    13621 gcagtagcat cttctataga attgtatacg aaaaaaacaa ttctataatg ctcataagta
    13681 tcatctagtc ttatttcata actaaattta tgataaaaaa gttcttttac tatttccgat
    13741 ctttggagta ttcccatccg atttcttaag cttagctcaa taggtgtttt tattggagaa
    13801 tcattttcac caattaattt atttttttta acagagtaaa tttcaagata ttttaatgga
    13861 aggaaaaaca caattatggt ttaaattcga tccttcgaat agatttgtca aagattttta
    13921 taaggtgtgg gattcagaag ttttcttttt agcaatcgaa gatagcttat taatcaatct
    13981 ctactattct aataagaact atttcaaaat tcctgctgcg aaaactagaa tgaagaaggg
    14041 tgtatacttt ttgtttgata tcgtgactga cgtgccggac gcacgtagcg atcatcggcg
    14101 ttatgactat ataaagtata ctttcgttga tccagaaaga tacaaagatt aaataaaaaa
    14161 ataacaaaca aactcgcttg aaattcatta ggtgagtttg tttattattt tttctgcttc
    14221 tcgattcatc agcgccatat ctgttttcgt aatggtttca ttaggaatat agtcagcaca
    14281 ttttcttttc tctaacattc tatacccact attatgtatt cctaaggtat tcaattcctt
    14341 aactgttgaa aacaaattcc aaacggtttg gtggcttcct tttccgctac ttccattata
    14401 aataatatca taattttctt tcaaatactc atcagatttt ttaaaagcac tgtagtatgc
    14461 tcttgaaaca gcagttcttc tataagattc tttagaatta tatttatcat ctgattcaat
    14521 ttgtttggaa atagataaat attcattaaa gtcaaacatt ttattccccc aaagttgcta
    14581 ctacagattt ccccatatat ttatagtcag ataatacatt atcgagagta tccaaaacat
    14641 cgtaatctga ggaaaacaat tcagatgttg ttttgataac aaagaaatac aagtttaaat
    14701 cttcatcata ttttatattg atttcagctt ttctttttaa aaccgtattt atcacatcaa
    14761 aaattttgcc atcattactt gaaattgtgt taatctcatt ttttgtagta attacaagtt
    14821 gattatcatt tttagttttc ggagtagaac ttgcatccac aacaactgga gatgctagaa
    14881 ttaccgatgc gcaagcaaaa atcgcagcaa aatttattaa gctaccactc cgatgtgtat
    14941 ttgatggtac tatatcaagc atccgtatcg tctccttaat ttttttccac tggtttgttg
    15001 accttgatat caaagatttg ccttccaatc tcagttccat taaatttaac aacagcataa
    15061 tgctctcctg cttcttcaac caagacattt ctggcgttta tattaaagtt tatatcgtta
    15121 acttgaggag gcaagcttac cgatacttca cccgtagaaa aaatttgttt ttcattcttg
    15181 tttaaaactt caatttctac tatttttatc ttttctgtat cctctatctc agacaccaca
    15241 gctgttacag aaaatgataa agcagtagga attaaaggcg cattaataga aagagctgga
    15301 ttaattactt tattaataac attagtgcca aattgctgac tttctacatc ttctgaaatt
    15361 attaattgta ctctataaga cattgatttc tcctaatttt tacatttatt taataaattt
    15421 atgtttcact tttgctagtc aatttagttt aacaatagta acaaagcttt gtcaacggaa
    15481 atattagcta attttgtaag catatttata agtatatttg taagcatatc tgacatttaa
    15541 tcagtagtaa aagtgcctct atataaggaa caaactgttt ttttactatc taaattcgta
    15601 tacagtttta ctaacaaatt agctaacaat attagcaact atatcaacaa ctatatgtac
    15661 ttcccctca
//
LOCUS       DACDEG010000056        15205 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.058, whole genome shotgun sequence.
ACCESSION   DACDEG010000056 DACDEG010000000
VERSION     DACDEG010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.058"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            93..1211
                     /locus_tag="IV142_001810"
     CDS             93..1211
                     /locus_tag="IV142_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8485467.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1339..2715
                     /locus_tag="IV142_001811"
     CDS             1339..2715
                     /locus_tag="IV142_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP8485468.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3116..4696
                     /locus_tag="IV142_001812"
     CDS             3116..4696
                     /locus_tag="IV142_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP8485469.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            4894..5117
                     /locus_tag="IV142_001813"
                     /pseudo
     CDS             4894..5117
                     /locus_tag="IV142_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5322..5642)
                     /locus_tag="IV142_001814"
     CDS             complement(5322..5642)
                     /locus_tag="IV142_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP8485470.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(5790..8024)
                     /locus_tag="IV142_001815"
     CDS             complement(5790..8024)
                     /locus_tag="IV142_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP8485471.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8503..8769
                     /locus_tag="IV142_001816"
     CDS             8503..8769
                     /locus_tag="IV142_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP8485472.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            8769..10496
                     /gene="ptsP"
                     /locus_tag="IV142_001817"
     CDS             8769..10496
                     /gene="ptsP"
                     /locus_tag="IV142_001817"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP8485473.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            10627..11706
                     /locus_tag="IV142_001818"
     CDS             10627..11706
                     /locus_tag="IV142_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8485474.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            11723..12946
                     /locus_tag="IV142_001819"
     CDS             11723..12946
                     /locus_tag="IV142_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8485475.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            13035..13922
                     /locus_tag="IV142_001820"
     CDS             13035..13922
                     /locus_tag="IV142_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP8485476.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTNTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            14008..14238
                     /locus_tag="IV142_001821"
     CDS             14008..14238
                     /locus_tag="IV142_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP8485477.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(14295..>15205)
                     /locus_tag="IV142_001822"
     CDS             complement(14295..>15205)
                     /locus_tag="IV142_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP8485478.1"
                     /translation="DRDGKVIYVGKAKRLKNRVSSYFHQNKQHSKKVLRMIHHITDFD
                     FVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVHITNKGFVLTDTPTARTYGP
                     FRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQEMTFEQKKKELQGLFQGRN
                     KKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRRIQEQKQFLRTPSLTFSMPL
                     AADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAHLSLKRQLSKEEIDPVQILI
                     SYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         4947 a         2610 c         3103 g         4545 t
ORIGIN      
        1 aaaagtatgg caagttttca aaaataaaaa aagaataaaa gaatttttaa cgaatataat
       61 atatactagt aagaaagaaa ggaggaaatc tattgtttga aaaacttaga cagtctaaac
      121 taatgttttg gtcgcttgaa ttattgatca tagctgcatt gatcatgatt tcatcagaaa
      181 taaactttat tttcgaaccg atcgggacat ttttttccac gttatttgca cctgtactga
      241 ttgcaggctt tctttactat ttattgaatc ctattgtcaa gctgttgatg aaagcaaaaa
      301 tcaaaagaat atgggctgtc gcaattgtgc tgctcctttt agttgcagca attatttgga
      361 ttttacttag tgtcatccca agtctagtac agcaaatttc ctcattggca tctaatatgc
      421 ctgatttcat caaacaggta gaaagctggt taaaagaggt agccgaattg cctttgttca
      481 aggaagtaga tatcaataaa tattttgagc aattagacat ttcttacggg acaatcattc
      541 agcaattcct tagtggagta tccaatagtt taggttcgat cgttggaacg atcgcttcgg
      601 caaccgtagt aatcattaca gcaccattca tcttgttcta tatgttgaaa gacggagaaa
      661 aactagtgcc aaacatccaa cagtttttcc cagaaaaaag aagaaaccaa attgtggagt
      721 tattggggca actgaataaa acattggcta attacattag cggacaagca atcgaatgtt
      781 tattcgttgg taccttcaca tttttaggat atatggtaat tggtgtggat tatgctttct
      841 tatttggggt cattgcggga ttgacaaacc taattccata tttaggacct tatctaggac
      901 tagcacctgc atttctagta acagtcttca atgatccggt aaaagcatta ctttgttgtg
      961 tggttgtgct aatcgtccaa cagttggatg ggaatatcat ttatcctaat gtaattggga
     1021 aatcactgaa aatccatcca ttaacgatca ttatcgttct tctagtagct ggaaatattg
     1081 ccggattgct tggtatcttc ttaggtgttc ctttttacgc aatctgcaag acgatcattt
     1141 cctatataat caaaatcgtc aaagaagaca agcaaaatga aaacaagaag aaaattgcta
     1201 cgacctcgta ggtagtttta gtacacttgt ttttattgca aagaaaattg ctttgtgata
     1261 ccattttaac gtggctatag tgccacgttt ttttctatcg taaactaaaa attaatttta
     1321 taaggagaga atgcgattat gaatgctgac cctgagagtc agtcgctgtt agcgcagatt
     1381 ttattattga tcgttttgac attgatcaat gcttttttag cagcttccga aattgctgtt
     1441 gtttcgatca ataagaatcg aatcgagcaa aaagctgatg aaggagatgt gaaatctcaa
     1501 aagcttttaa aaatattgca gaatccaaat aatttcctat cgacaatcca agtaggaatc
     1561 actttggtca acattttgtc tggtgcttca ctagctaata ctttatcgga acggttagct
     1621 ccggtattag gtggcggcac agcggcaaaa aatattgcaa atatcattgt gctggctatt
     1681 ttgacctatg tatcgattgt ttttggagaa ttgtacccta aacgtattgc tttaaataaa
     1741 tcagaagaag tcgcaaagtt cacttcaggg ctgatacgct tgattggtgt agtagctaag
     1801 cccttcgtct ggctgctttc tgcttccaca agtttattgg cgcgtatcac gccgatgact
     1861 tttgatgatg aagattcaaa aatgaccaga gatgaaatgc gttatatgct tgaaaacgaa
     1921 ggcgtattga ataatgaaga attagaaatg ctccaagggg ttttttctct tgatacgaaa
     1981 gttgcccgag aagtcatggt cccacgaacg gatgcattta tgattgatat caacgacact
     2041 gtggaagaaa atgtgaatga agttttatcc gaaaactatt caagaatccc tgtttataac
     2101 gaagacaaag ataaagtagt cggaatcctt catacaaaga atttactaaa agctgcccat
     2161 aaattcggct ttgataattt agatatcaag aaaatcatgc aggagccttt attcgttcct
     2221 gaaacggtct ttattgatga tctgttatat gaaatgaaaa agacgcagaa tcagatggct
     2281 attttattag atgaatatgg cggcgtcgtt gggttagcaa cactagaaga ccttttagaa
     2341 gaaatcgtcg gagaaatcga tgatgaatca gatgaagttg agaatcttta tgaaaaaatc
     2401 gatgaacacg aatatatcat ccaaggacgt atgctgattg atgagttcaa tgaagctttt
     2461 gacagtgatc tgcatatgag tgatgtagat acgatggctg gttatttgat tacagcatta
     2521 ggcatgattc cggatgaagg cgaaaagtta tcgtttgatg tcgataatat tacgctggtt
     2581 tctgaagaaa tggaaggttc tcgaatcctt aaaatccgtg tcatttttca tgatccggaa
     2641 gaaacagaag cggaacctga agaagaacga agatacttca gaaaagaatt cgaagatgac
     2701 gagccaagaa gataattgga aacttggtta tgaaagacgt gttgcttgaa taaacaagca
     2761 acacgtcttt tcttttagtc agctgacaaa aaaatatttg actcttatgg tttggtggct
     2821 ttttcattcg ctgaatccat gctatactga ttcagttgta gtagttgaca aaggttgtga
     2881 aaaagctatc ctgcattaag taataagagc agccaagcaa aaacagcttt tcgtattttt
     2941 ggcttgggtg ggcttattgc tgataggctg tctctagaac gccgtttact cggaaaaaga
     3001 gactgctaca cggcttttgt cacagcccta tatgaaattg tctttcttta agagatcgga
     3061 caatctgtgc ctagagaaaa tataaataat tgaatggaag aaatggagtt aggttatgaa
     3121 taatccaaaa ttaaagcaac aagtggacaa tcgcagaact tttgcgatca tttcccatcc
     3181 ggatgctggg aaaacaacga ttacagaaca attactattg tttggtgggg ctatccgtca
     3241 agccgggacc gtaaaaggca aaaaaacagg taattttgcc aaatcagact ggatggaaat
     3301 cgagaaacaa cgtgggattt ctgttactag ttctgttatg cagtttgatt atcaagggaa
     3361 acgagtaaat atcttggata caccaggaca tgaagatttt tctgaagata cttatcgtac
     3421 tttgatggcc gtggatagtg cggtcatggt aatcgatagt gcaaaaggga tcgaagcaca
     3481 gacaaagaaa ttattccaag ttgtaaaaaa acgtggcatc ccgatattta cttttatcaa
     3541 caaactagat cgtgatggtc gtgaaccatt ggaattgtta gaagaattag aagagctatt
     3601 agatatcgaa tcttatccga tgaattggcc aatcggtatg ggtaaagggc tagaaggtct
     3661 ttatgatatc taccataatc gtgtagagtt ttatcgtccg gaaaattatc aggatgaacg
     3721 attagtagaa ttggatgaag atggactgct tcccgaaaat catcctctaa ctgaaaattc
     3781 cttatatgaa caagtcttag aagaagtaga actaatcaaa gaggctggag atgcatttaa
     3841 tcaagaaaaa attgctcgtg gcgatcagac accagtattt tttggttctg cattgactaa
     3901 cttcggtgta cagacttttt tagagacttt cgttgaattg gcaccagctc cttatggaca
     3961 caaaacacaa gatgatcaga tggtaagtcc ttatgaggaa gaattttcag gtttcgtatt
     4021 caagatccaa gcaaatatga atccagccca tcgtgaccga atcgcttttg tccgaatctg
     4081 ttctggaaca ttcgaacgag gaatggatgt ctggttggaa cgtacaaata aaaaactgaa
     4141 attaagcaat gtcacacaat ttatggcaga ttcgagagaa aacgtcgaaa aagcagtagc
     4201 aggcgatatc atcggtgtat atgatacagg caattatcag attggggata cattgttcga
     4261 aggaaaacta aaagttgctt atgaagaact cccttcattt actccagagc tgtttatgaa
     4321 agtgactgca aaaaatgtca tgaaacaaaa atccttccac aaaggaatct atcaattagt
     4381 tcaagaagga gcgattcagc tttataaaac atatctaacg gaagaatata tcattggagc
     4441 tgttggtcag ctacaattcg aagtattcca atatcgtatg ttaaatgagt ataacgccga
     4501 agtaatcatg tctccaatgg gacacaaaat cgctcgctgg atcgatccag aagatttgga
     4561 tgagaagatg agttcaagtc gcaatatctt ggctcgtgac cgtttcgacc aaccgttgtt
     4621 cttattcgag aaccaatttg cagaacgctg gtttgcggat aaatatccca atgtaaaatt
     4681 aaaaagtttg atgtaaagaa gtcttgacac aagctgccct gcatcaagta ataagaatag
     4741 ccaaacaaaa acagcctctc atattttgtt tagagccgtg acggattttc cgtgacggct
     4801 ctttttacta tattaaaatc atagaaatgt ctaatcaaaa aaatataaaa attattttgc
     4861 gtacatatga ttaaagaaag gagagtgtga cacatggaaa cagaatggca aagacgtgaa
     4921 agaaaaaaac agaaaaagaa gagatgttac gattatgtcg attattagat gtaaaagact
     4981 gggacaaaaa cgagcaaatt gcacaaaaag tacgatcaat catccataaa gaggaaaaag
     5041 ttaaaaataa agacacgcgt agaactgaaa atgaaaagag aaaagtacaa aagaaaagag
     5101 aaaaatcaaa aagctagaaa gaaaaagagg ctgagacaga agcgtttaac tccaagaaat
     5161 aagctggaat tcacaaaaat ttgaaaagca attttcgtga atttcagctt atttccgaag
     5221 gagttgcttc tgctttcgcc gtttatacgt gtttagagcg tgagacaaaa gtgttcttta
     5281 cttttgtccc acgctcttta attattatgt tcaaaatttg tttacacggc taatgtagcg
     5341 cctaattcat gttttggaat cgagatttca acaaggcctg ccaattcgat tactttgatt
     5401 tcatcctcgt ggtaaacggc gtctttgatt ttatctaaga aataattttt agcatcttcg
     5461 atttctttcg gctggatttt acgtttgtca ttgatcagca gaatttcatt atgttcaggg
     5521 gcttcagtgt agacaatagt ggtatttccg gctgtgtaga ccttgactag atgggcatca
     5581 gtgttttcaa gctggtttaa tacaagacgg gaatggctgt ttgttacatt taccaatttc
     5641 atggcgctca cctcttttat gtatttttaa ttgcttgttt ctcttattat aatttctttt
     5701 ttataaaatg aacagtattt tgacttttct gaaaaaagaa tgagacggca tttctcaaag
     5761 aaataccgtc tcacgctata aaactggatt tattcagccg tttcttcggc tgattcttta
     5821 gccaaacgtt ctggtttaga agtaacgatg atttttccat ctttatccag ttttgctttc
     5881 aactcatgga tagaagggtg atcaagataa aattcagcga taccatcttc gatttgttct
     5941 tggatcgtgc gtcgaagagg acgagcaccc atagatggat catagcctaa gtcaactaat
     6001 ttttctttga cattcgtagg aacttcgatg tgaagttgct gtgctgccag catttggttg
     6061 acatcgtcta gcattagtgt gacgattgtc atcaagtttt cttttgataa tgcactgaat
     6121 tcgatgatcc cgtcaaaacg gttcagaaat tctggtgtaa agtagttgtt taattggttc
     6181 aatacagaac gagtaacacc ttcgcgtgcc gcaccaaaac caacgtttgc ttctacttta
     6241 ccagttccgg cattacttgt cataatgatg atcgtatctt taaagctgac cgttcttcct
     6301 tgtgcatctg tcaatcgacc atcatctaag atttgcaaga acatatgcaa aacatctgga
     6361 tgagcttttt ctacttcatc taacaagaca agactgtaag gatttctccg tactttttct
     6421 gttaattgac cagcttcttc gtatccgaca taacctggag gagaaccaat caatttagag
     6481 acactatgtt tttccatata ttcagacata tcgaaacgaa tcattgaatc ttgtgaaccg
     6541 aatagttcgt aggcaagttg tttcgcaagc tctgttttac ctacaccagt tggtccaaca
     6601 aataagaagg agccgattgg acgattctgt ttgtttaatc caacacggtt tcggcgaatc
     6661 gctttcgcta ctcgatctac agcgttatct tgtccgatga catgagcttt caagtcatct
     6721 gctaagtttt tcagttgtgt ctgttctttt tcttttaatt cacccactgg aatacctgtc
     6781 cgttgttcaa cgattttttc catatctttt tcggtaatca caggtgtttc ctcttcagtg
     6841 agctgacgtt ctttcatttt ttgaagttta ttgatctgat cgcgataata agctgctttt
     6901 tcgaaatctt cttctcgaga agctaaaact ttttgttctt ctgcttcttg cagttttttc
     6961 tcgattgttt taggatcaac gatttggatc gtcaagtttt tcttagaacc tgtttcatct
     7021 agcaaatcga tggctttatc cggcaagaag cgatcttgga cgtaacgatt ggataaagtt
     7081 gcggctgctt cgatggcctc atcagtatat tttacatgat gatagtcttc ataacgtttt
     7141 tgcaaacctt tcaaaatact gatcgtttct tcgacagttg gttcatctac ccgtacaggt
     7201 tgcatacgac gttctaaagc agcgtctttt tcgatgatgc ggtactcatt taaggtagta
     7261 gcaccaacca tttgcaattc tcctcgagct aaagctggtt ttaaaatatt accagcatcc
     7321 atatttccat caccagcaga accagcaccg acgatttcgt gaacttcatc tatgaatagg
     7381 atcacatttt ctgcttgacg gatctcttca atcaattttt gcatacgttc ttcaaattgt
     7441 cctcgtatac cagtaccttg gactaaagat acgacatcta aacgaatgac ttctttgtcc
     7501 attaattttt gcggtacatc accatctacg attttttgtg ctaaaccttc aacaactgct
     7561 gttttaccaa cacctggttc tccaatcaag acaggattgt tcttggttcg gcgatttaaa
     7621 atttcaatga ctcgtttgat ttcgtcatcg cgtccgatca caggatcgat atcaccttgt
     7681 cgtgcttgtt ctgtgatatt gattccgtat tctcccaata aaccttctgt attgccttga
     7741 ggtggctgac caccattgaa gttgttgttt ccgccaaatt gtgttggtgg tgtttgctca
     7801 taatttcctt gctgttgcat ttggcgagac atggaacgga aaagatcgtc caaactgcca
     7861 aatccaaatg gatcattttg ggccatatta gtcagacctc cattttgttg atttttgatt
     7921 ttttgataac agctctgaca gtaatcgagt tgtgtccgct gaccattgac agtggcatac
     7981 aagtgaatcg ttgcctcatt cttgccgcag ttttgacaaa gcatgaatca ttcacgtcct
     8041 ttcacaaaaa tgggtacatc catattttac atctcgtttt tcatgaggta aaagaataac
     8101 ccctagtttt ttaagagatt tatgaggtag aaaaggtgaa atctctattg accattcctg
     8161 acctttgact cttattatac taaaaataaa aagggaagca agtaattcgt ttcatatgaa
     8221 aacaaagagg gtcaaaggaa agaatgataa acgtcaggag aagagaaagc gacaagaaaa
     8281 aggaaataaa cacgtttgtt ataaaatgtg aagaaaagga tgaaaaagaa tccggaataa
     8341 tttgaatact tttatctgaa attgaatgaa aactgttgta tctaacgaaa aaaaatgtta
     8401 atctttacag atgaaagggt ggcatttgtt aggattctct gaattactaa aaaagaaaac
     8461 cctcacataa aaacaacact cacttaaagg agaccgatta atatggaaaa gaaagaattt
     8521 cacgtagtag cagaaactgg gatccacgct cgtccagcta cactattagt acaaacagca
     8581 agcaaattta actctgatgt aaacttagag tataaaggta aatcagtaaa cttgaaatca
     8641 atcatgggcg ttatgtcttt aggtgtaggt caaggttctg atgttactat tactgcagaa
     8701 ggtgctgacg aagctgatgc aatggcagca atcgttgaaa caatgaagaa agaaggctta
     8761 tctgaataat ggttgaaatg ctaaaaggga ttgccgctag tgacggtgta gccgttgcaa
     8821 aagcttacct gctagttcaa ccggatttaa cattcagtaa ggcaacagta gaagatactg
     8881 cggctgaaga agctcgtttg gatgctgcat tagctaaatc tactgaagaa ttgcagcaaa
     8941 ttcgtgaaaa agcagcgcaa agcttaggtg aagcagaagc gcaagtattt gatgcccatt
     9001 tgatggttct ttcagatcca gaaatggtag gtcaaatcaa gcaaaatatt aaagacaaca
     9061 gcgtaaatgc tgaatcagcg ctaaaagaag taacggatat gtatatcggt atgttcgaag
     9121 caatggaaga taatgcttat atgcaagagc gtgctgcaga tattcgtgac gttgctaaac
     9181 gtattttagc gcacttatta ggcgttactt taccaaatcc atcaatgatc aacgaagaag
     9241 tggtcgttgt tgcacatgac ttgactccaa gtgatacagc acaattagat cgcaattttg
     9301 taaaagcgtt tgtaacagac attggtggac gtacttcaca ttctgctatc atggcacgtt
     9361 ctttagaaat tccagcaatc gttgggacaa aagaaattac tgctaaagtc aaagaaggcg
     9421 atattttggc tgtcaacggg atcgaaggcg atgtaatcat cgatcctaca gatgaacaaa
     9481 aagctgaatt tgaaaaagca ggtgcagatt acgcagcaca aaaagcagaa tgggaaaaat
     9541 taaaaaatgc agaaactgtc acagctgacg gcaagcattt tgaattagct gcaaacatcg
     9601 gaacaccaaa agatctagtt ggtgtccata ataatggtgg agaagcagtt ggtctgtacc
     9661 gtactgaatt cctgtatatg gattctccag acttcccaac agaagatgac caatatgaag
     9721 cttacaaagc tgtattagaa ggtatggaag gaaaacctgt agttgttcgt acaatggaca
     9781 tcggtggaga taaagaacta ccatatctgc aattacctca tgaaatgaat ccattcttag
     9841 gatatcgtgc attgcgtatc agtttatctg aacaaggcga cgaaatgttc cgtacacaaa
     9901 tgcgtgcatt gcttcgtgca tctgttcatg gtaatttacg tatcatgttc cctatggtag
     9961 ctacactcaa agaattccgc gcagcaaaag cgatctttga agaagaaaaa caaaaattga
    10021 taagtgaagg caaggaagta tctgatacga tccaagtcgg catcatgatc gagattccag
    10081 cagcagcagt cttagcagac aagtttgcaa aagaagtaga tttcttctct gtaggtacga
    10141 acgatttgat tcaatacaca atggcagcag accgtatgaa cgaacgcgtt tcttacttgt
    10201 accaaccata taacccatca attttacgtt tgatcaaaaa tgtaattgat gcagcacacg
    10261 ctgaaggaaa atgggcaggt atgtgtggag aaatggctgg agatcaaaca gctgttccat
    10321 tgctagtagg tatgggtcta gatgagttct caatgagtgc gacatctatt cttaaaacgc
    10381 gtagtttaat gaaacgcttg gacacagcga aaatggctga acttgctgat cgtgcattga
    10441 aagaatgcga tatgatggaa gaagtcgttg aacttgttca tgaatatgta aaataaacaa
    10501 cgtatcaaaa gctggtgtgg cttgtaaaag agctacgcca gtttttttgt tcttttttaa
    10561 gaaaacgttt ttgtcagtaa gaatagacaa aaatagggct taagtttgag tgacagtttg
    10621 taggctatgt tatactatat aagtgaaggg ggacgtaagg tgaaggtatt attatatttt
    10681 gaaagtgaaa agatgcttgc aaaatctgga atcgggcgtg cgcttgatca tcaaaaacgt
    10741 gctctgacgg aagtcggtat ctcttataca ctagatgaaa aagaagatta tgacatcctg
    10801 cacatcaata catacggaat caatagtcat aatatggtca ataaagccag aagagaagga
    10861 aaaaaagttg tctatcatgc gcattctaca gaggaagatt tccgaaattc ctttatcgga
    10921 tcgaaccagc tttctcctat cgtaaaaaaa tatctggtag gattgtacca aaaagcggac
    10981 tatctgatca cgccaacacc ttattcaaaa cagttgttgg aaagctatgg tatccgtgtg
    11041 ccgattcaag ctatttccaa tgggatcgac ttagagaaat atcaccctga tccaatgaaa
    11101 gaacaaaagt tcagagaata tttcaaactg tctccggatc aaaaagtaat catttgtgtc
    11161 ggtttgtttt tcgaacgtaa aggaatcatt gatttcgtag aaattgctaa gaagatgccc
    11221 gaatacacat ttatttggtt tggtcatgtt cccatgtact cgattcctcg gaacatccgg
    11281 aaaattgtaa aagaagatca tcctgaaaat gtcctgtttc ctggttacat tcgtggagag
    11341 atcatagaag gtgcgtattc aggtgcagat ctattctttt tcccttctta tgaagagaca
    11401 gaagggatcg ttgttttaga agctttagca agcaaacaaa acgtacttgt tcgagatatt
    11461 ccagtgtata atggctggtt agaagatagc aagaactgct atatggggaa gacaaacgaa
    11521 gaatttatcc gactgatcca acagattacc aatcaagaac taccgagtac gacacaagcc
    11581 ggttatcaaa cagcaaaaga gcgaagtatc aataaaatcg gtgaagaact taaaaatgta
    11641 tacgaaagtg tactgaatga aaaagtctct agcaaattaa aaaaagtaaa tcaggtcaag
    11701 gactagtttg gaggcgaaaa acatgaaaat cggctttttt accgatacct actttccgca
    11761 agtcagcgga gttgctacat cgatcaaaac attaaaacaa gaattagaaa gacatgggca
    11821 tgaagtctat atttttacaa cgaccgatcc taatgcggat aagttggaaa aagatgtgat
    11881 tcgaatgcca agtgtaccat ttatttcttt caaggatcga agagttgttg ttagaggcat
    11941 gtggtatgct tatttggttg ccaaagaact agaactagat cttattcata cccatacaga
    12001 attcggagca gggatacttg ggaagatggt agctaagaag ttgaaaatac ctctgatcca
    12061 tacttaccat acgatgtatg aagactacct tcattatatc ggtaaaggaa aagtgatcag
    12121 gcaatcccat gtaaaatact tgtctagatt atttgcaaat catacgacag gtgtagtttg
    12181 tccaagtgaa cgtgttattg atacattacg cagctatggt gtaatagcac cgcttcgcgt
    12241 gattcctact ggtatcgaca ttgaaaaatt caaacgttct gatatcactc agcaggatat
    12301 cagcaaatta agggagtcac taggtcttca ggaaaaccat ttgatgattc tttctttaag
    12361 caggatttct tatgagaaaa atatccaagc cttgattaat gggtttcctc aggtcatctc
    12421 cgcttatcct aacgccagat tagttatcgt aggaaaagga ccttatgtag aagagttaga
    12481 ggaattgatc tcagaactgc agttagaaga atttgtgcag ttcacaggag aagtggacaa
    12541 caatgatgta gcactttatt ataaagcggc agattatttt gtcagtgctt ctacttcaga
    12601 aacacaaggg cttacttata cagaagcgat ggcagccgga acaccttgtg tcgtggaagg
    12661 aaatgcttat ttgaaccgat tgttcgatca tccttcatta gggaaaactt ttgaaaccga
    12721 cgaagacttt gcaccagcac ttattgatta tatttccagt gaagttccta aagatccaaa
    12781 agttttagaa gctaaacttt atgatatttc atctacacat ttcggcgaag ctatgatcga
    12841 attttatcaa gacatggttg tttatcatga agaagagatg cgaagaaaag aagaagaaga
    12901 ctcgattgaa cgaatcaaaa taaaattaaa ttcatttaaa agataaaatt caaacatttt
    12961 ttatgaaaaa atccttgttt ctagcgtata ctagcataga agcaaggatt tttgattttt
    13021 aggaggtcga aaaaatgaaa cttggtttta tcggaacagg cgtcatggga agtgctgtcg
    13081 cacgccatct actagaagca ggacacgaag tagctgtcta taatcggaca aaagcaaaag
    13141 ctgatccttt agtaacagaa ggagctatct gggcagatac accaaaagcg gtagcagaac
    13201 aaagtaatat tctctttaca atggtaggtt accccaaaga tgtagaagaa atctactatg
    13261 gacagtcggg gattttttct gcagatatct caggacatat tttagttgat ttgactacaa
    13321 atacaccaag cctggcagaa aaaattgcaa aaacagcaaa agaaaaaggc gcagatgcgt
    13381 tagatgcacc ggtatccggt ggtgacttag gagcaaagaa tggtacactg acgatcatgg
    13441 tcggtggaga agaagccgtt tatgatcaag tactgccttt attcaaagaa tttggtacga
    13501 catttacatt gcatggttct gctgggaaag gtcagcatac aaaaatggct aatcagatca
    13561 tgattgcagg tacgatgaca ggaatgacgg aaatgcttgt ctatgcacaa aaaaacggac
    13621 ttgatttaaa gaaagtgatt gagacgcttt caggtggaag tgcagctaac tggtctttaa
    13681 gcaattattc tcctcgtatt ttaaaagaag attacacacc tggttttttt gtcaaacatt
    13741 ttatcaaaga tttaaagatt gcattagaag aagcagaaaa aatggatctt gtattgccag
    13801 caactaccca agcattgaaa ctatatgaag agttggcaga taaaggtttt gagaatgacg
    13861 gaacccaagc tttgatcaaa ctttggtggc ctgaaggaaa aataccagaa aaaaaatcat
    13921 aaaaaacgag tttttttaga gaaaatcaat gcaagtaggc tatgcttttg atacaatata
    13981 acagaaataa gaaaaggagg acctccgatg acacattcac aattagtcgc aatcataaaa
    14041 cgactggagg ctatggtcga gtcagcagat aatgaactgc aagttcgccg ttttgaaaaa
    14101 gaaggcgtag agaagtgtac agtaacttat gataaagcga ccgatacatt tgaattaact
    14161 gaaacagata ctcatcagca atacgaattt gataatatcg atatcgtagc gatggaaatc
    14221 tatgacttaa tccaataaat tttaggataa ttgacatttg aagttgaaga tgcctaaaag
    14281 aagccgagaa gcaactaatt tagttgcttt tcagcttctt ttttgtttaa cagttcaatt
    14341 tcaaactgtt ctttttctaa ttttttgcgg taactgatca agatttgtac aggatcgatt
    14401 tcttcttttg acaattgacg tttcaaagat aaatgagctt ctttcttttc ataatagaag
    14461 tcaggagaaa cgtcaccaga ggtaatcatt gtttctgcaa gttgaccata acaaacgagg
    14521 tagtgctttt tctgactctc atctgctgct aatggcatgc tgaaggtgag cgatggtgtt
    14581 cttaaaaact gcttttgttc ctggatccgt ctaataaaat aagtaactaa ttcgatatct
    14641 ttttgaagca taccggcttt ttcgaaattc aactgataga tgaaatgttg ttgtcttttt
    14701 ttcaagtaag tcagtagctt tttatttcgg ccttgaaaca gcccttggag ttcctttttt
    14761 ttctgttcaa aagtcatttc ctgtaggtca ggaagctgga ctcgcaaagc taacaaacta
    14821 atttcagaca accaaggcat ttgataagtt tcttccatta tacgcaaaat agacggcatt
    14881 tttttataga gacgaaaagg cccgtatgtt cgagccgtag gagtatctgt taatacaaat
    14941 cccttattgg taatatgtac ataattataa ttactgaaat aattcatttg tcggttataa
    15001 aaaggacggt aatgttggat caattgacac tcgagcaata aagcatctaa ttccgtgtct
    15061 actacgacga aatcaaaatc tgttatatga tgaatcatac gtaatacttt ttttgaatgc
    15121 tgtttattct gatgaaaata gctgcttact ctatttttga gccgttttgc tttaccaaca
    15181 tagatgacct taccatctcg gtctt
//
LOCUS       DACDEG010000057        15177 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.059, whole genome shotgun sequence.
ACCESSION   DACDEG010000057 DACDEG010000000
VERSION     DACDEG010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.059"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(90..1193)
                     /locus_tag="IV142_001823"
     CDS             complement(90..1193)
                     /locus_tag="IV142_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP8485479.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(1415..1885)
                     /locus_tag="IV142_001824"
     CDS             complement(1415..1885)
                     /locus_tag="IV142_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP8485480.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            2142..3461
                     /locus_tag="IV142_001825"
     CDS             2142..3461
                     /locus_tag="IV142_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAP8485481.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            3902..5542
                     /locus_tag="IV142_001826"
     CDS             3902..5542
                     /locus_tag="IV142_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP8485482.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            5696..7018
                     /locus_tag="IV142_001827"
     CDS             5696..7018
                     /locus_tag="IV142_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP8485483.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(7080..7511)
                     /locus_tag="IV142_001828"
     CDS             complement(7080..7511)
                     /locus_tag="IV142_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8485484.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(7575..8411)
                     /locus_tag="IV142_001829"
     CDS             complement(7575..8411)
                     /locus_tag="IV142_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP8485485.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(8601..8903)
                     /locus_tag="IV142_001830"
     CDS             complement(8601..8903)
                     /locus_tag="IV142_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP8485486.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(8929..9360)
                     /gene="ruvX"
                     /locus_tag="IV142_001831"
     CDS             complement(8929..9360)
                     /gene="ruvX"
                     /locus_tag="IV142_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP8485487.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(9357..9623)
                     /locus_tag="IV142_001832"
     CDS             complement(9357..9623)
                     /locus_tag="IV142_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP8485488.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDSAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(9877..10602)
                     /locus_tag="IV142_001833"
     CDS             complement(9877..10602)
                     /locus_tag="IV142_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP8485489.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(10968..11038)
                     /locus_tag="IV142_001834"
     tRNA            complement(10968..11038)
                     /locus_tag="IV142_001834"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11004..11006),aa:Gly,seq:ccc)
     gene            11155..11907
                     /locus_tag="IV142_001835"
     CDS             11155..11907
                     /locus_tag="IV142_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP8485490.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            11923..12402
                     /locus_tag="IV142_001836"
     CDS             11923..12402
                     /locus_tag="IV142_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP8485491.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(12653..14233)
                     /locus_tag="IV142_001837"
     CDS             complement(12653..14233)
                     /locus_tag="IV142_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP8485492.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(14226..14969)
                     /locus_tag="IV142_001838"
     CDS             complement(14226..14969)
                     /locus_tag="IV142_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8485493.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
BASE COUNT         4580 a         3086 c         2681 g         4830 t
ORIGIN      
        1 caaattattc tattagtgac atccttgaac tataaaaata ggagtacggc atcatctgcc
       61 gcactcctat ttttttcgtc atttgtttct taaataaaac gattctcacg caattttatt
      121 tttttgatat cttctactgt ttgtgttcca gctaattgca tgaccatttc caattctgtt
      181 ttaaagaaat caaacacttg atggactcct gtacttcctc ctaaagacaa tccataaata
      241 acgggtctgc caatagcaac aagatctgca cctgatgcga ttgctttgaa aacatgctgt
      301 ccgcgtcgta caccgctgtc aaacacgatt ggtacacgtt tatctactgc ttctgccaca
      361 tattgcaacg aatcaaacgc tgctggacca ccatctagtt gacggccacc atgattagat
      421 acccagattc cgccagcacc agattctaat gaacgataga catcttcttc tgactggaca
      481 cctttcacat agactggcaa atcagaatga gcagcaataa attctacatc ttttggacta
      541 agcttctgtt tggaagattt gtacacagca tccatcgttt ggcctactcc tgattgataa
      601 gcctgaacga tcggcattgg cagtgggaag gtaaacccat ttctgcggtc tgtttcacgg
      661 tttccgccaa ctgttgcatc cgctgtcaat acgattgctt ttgctccatt acgcttggcc
      721 acttcaagaa tatccaaatt gatcccatca tctttgctca tataaaattg aaaccattgc
      781 ggtgctttct ccccgcctgc ttctcttatc tcttctaatg tacaagaagc gtaagaactt
      841 gctgtataga tcgtcccaaa atctgctaca ccttttgcag aagctttctc cgccttcaca
      901 tgagctaagc catgagcagc aactggtgcc atgatgattg gtgcagtcag cgtttctttg
      961 tcaaaatgtg tagttgtatc gggtaattct acatctctta atacatgagg aataatcagc
     1021 tgatgattga atgcccgttc attttcttga taagtaaata agtcgccagc accactgctg
     1081 atgtaaccat aaccaccagc tggaatcact tgtgaagcgg ctatttctaa atcttccata
     1141 ttgataaaat ccactattcc ttcatttgta cctgcttgat acgttttttc catttcaatg
     1201 ccttccctct ataaaaaatt gaatgaataa tatgatagcg tttacacact ttatattact
     1261 cttccgtatt gatacaggca aataatctgt ttacatttca gtcattccag ttgttactac
     1321 tctgaaagaa atgtttagaa cacaaataga taaaaaacga tgggattttg acaaaagtcg
     1381 tgtcaaagtc ccttttacga aatactgtgg atgcctattg aaataagtgc gggttgtttc
     1441 tttgtatttc aatcgtttct tgccattctt cgtttaattt tggattaccg ttttcttcct
     1501 tgaacgcata aggtcccaat ccaaaaagct cagttagtat ttgattcacg attccatata
     1561 gctcggtgtg atcggacaca tgattattga agatcatctc attatcacca cgtttgaatc
     1621 tcacattcgg gctctcatga tgaacctcta tgcagttttc cccttttttt aactgacgga
     1681 tttctgaaaa catctcataa atatcttctc caataaaacc ttcagcaagc gccagactgt
     1741 ccacaagacc gcctactgtc aactcagaga ttatcgcttt gttggcacca agctttaatg
     1801 tatagcttgt tgcttctact acgccgcatg cattgcaacc accttctgat agttcgcttt
     1861 ttctgatcaa ttgaacttct ttcattttct gttcctcctc ttagttttct acttatgtat
     1921 tttttatcac ctgacgtaag tgtattttat cgtcacgatc acgtgattta ttcttcagag
     1981 attctacctg aataattcat acttttaatt aaaaccatgt gaaactgcct cacggcagct
     2041 tacaattact ttttcatctt cacccgttaa gtgatgaaaa aagaacccct ttctgctatg
     2101 gttaaagtga ctaaaccaac caaaagaaag gagttctttt catgactagc ttacacaaaa
     2161 accaagtaaa attcaattca aatatcacta tttctcatac aggtggtcaa ttatcgagtg
     2221 attcgggtct cgtcctagtg aaagagctga tgaacatctt tggtttttct gaactggcta
     2281 agcaacacat tcacattgaa gacgaacgag cgtattttac tcatgacaac ttatccatac
     2341 ttgagcagtt cattatgcaa ttaattgctg gttactcagc tgattctgca gctaatctcc
     2401 tgagacaaga tcctgtgttt aaagctgttt tagataggaa agaactcgct tctcaatctt
     2461 cactttcccg atttttagat cgactatctg aggagaatat ccatgaactt caagctttga
     2521 accaagaact tatcgataaa gcacgcctca tccgtaatga tacggaatta atcattgatt
     2581 tagattcgac ccattcagat acatttggtc accaagaaca aacgaattat aatacacact
     2641 accaaaccta tggttaccat ccattggtag cttttgacgg attgactggt gacttcttaa
     2701 aagctgaact acgttcagga aatcaatata catcaaaagg cgtaaaggag tttctcacac
     2761 ctttattaga gcactataat cactctctac caaacactga catcttggtt cgtggagaca
     2821 gcgggttcgc tacacctggt gtgtatgatt cgtgtgaatc aaaaaagagt cattatgtta
     2881 ttcgactgaa gaataatcgt agactaggtc aaatagctga gaaatcagta ctttatggcg
     2941 ataatcaaaa gtgggaagaa cgagaagttc agtacttctc gaccacttat caagcacaat
     3001 cctggtcaca aagtcgtcgc gtgtgtatac gctcaacacg tgaagcgggc gaactactct
     3061 ttcgacatga atttatcgtg acgaatctat cagaaaatgt ttctcctgat accatctttt
     3121 ctatctatgc caaacgtggc acaatggaga acttcattaa agaagcgaaa gctggctttt
     3181 actttgacaa aacagacagt cctcgctttt tggagaatca tgtccgaatg atgctcagct
     3241 tgatagctta caacttagtc aacttcttaa gaacgattgg ctttgaagaa gtccaaaagg
     3301 gaatgaccat tcattctatt cgattgaagt ttctgaaagt tgctgggaaa ttagtccaaa
     3361 cgggtagacg agtctatctc aaattatcta gttatcatgt gtatcagaat gaattctaca
     3421 gggtctttgc tcgcctgagg cgagccagtc aatggatcta atccaacaat ctaacgattt
     3481 ttttactggg aagttatcca gggagaagta tgctcaaaat gccttacacc caaaatttat
     3541 tcctcaatat ttttgaacag tgaatgtttg aagtaacttt ttagtcaaaa tcagttgata
     3601 gggatattag tgatacatat ttcaataaaa tgtgccaaag aattaaagct atgaattatt
     3661 caggattcta tttatctatc taacatatcc tttattcttc tacttttctt tctttagcat
     3721 ataagagata cgcatagtaa tcaagcaagg attgttgata taaaaagaac aaataccgct
     3781 attttgattt cttttgactc tattactcat ataaataaaa tttattatac ctgctctttt
     3841 tgactatatc ctcatctgac tcctatactt cgtttgtatt aaaacttagg aggaatcatt
     3901 catgcttaca gggctaaact tattaaataa tccttttcta aataaaggaa cagcttttac
     3961 aaaagaagaa cgggcaaaat acggaatcac tggtatgctt ccaagtactg tccaaacatt
     4021 ggaacaacaa tctgtccaag cttacggtca atacttaagc aaacagaccg atttggaaaa
     4081 acgtatcttc ttaatgaatc tattcaatac gaaccggaca ttgttttata aattgatggg
     4141 cgaacattta gttgaattca tgcctgtcgt ttatgatcct gtcgttgcgg attcaatcga
     4201 acaatacaac gaaattttct tgaaaccaca agatgctgta tttctttcaa tcgacgaacc
     4261 agaaaatatc aaagcaagtt tgaaaaacgc agcagatgga cgcgacattc gtttgatcgt
     4321 tgtgacagat gcagaaggaa tcttaggtat gggtgactgg ggtgtgaacg gtgttgacat
     4381 cgctatcggt aaattgatgg tctacactgc cgcggctggt atcaatcctg cacaagtctt
     4441 gcctgtatca atcgacgctg gtactaataa cgaagaatta ttgaataatc cattatactt
     4501 agggaatcgt catgctcgtg tagaaggcga aacttactac gaattcattg atcaattcgt
     4561 tcaatcagct actgaattat tcccagaatt gttattacat tgggaagatt ttggacgagg
     4621 aaatgctgca acgatcttag aaaaatacga agataaaatc actactttca atgatgatat
     4681 ccaaggcaca ggaatcgttg tactagctgg tgtattaggt ggattgaata tatcaggtga
     4741 gcaattgaaa gatcaaacga tcttgacatt tggtgctggt actgctggtg taggtatcgc
     4801 aaacatcctt ttagatgaaa tgatccgtca aggtgtacct gaagaagaag cacgccaaca
     4861 tttctatcaa gtagataaac agggtttatt gtttgaagat acaaaaggat tgactcctgg
     4921 tcaaatccca ttcgctcgta aacgctcaga attcactaat gctgatgaat tgacaaactt
     4981 agaagcagtt gtaaaagcaa tccatccaac gatcatgatc ggtacttcta cccaacctgg
     5041 agcattcact gaagaaatcg tgaaagaaat ggcggctcat accccacgtc caatcatcat
     5101 gcctttgtct aatccaacaa aattggcaga agcaaaagca aaagatttga ttgaatggac
     5161 ggacggaaaa gctttagttg gaacaggtat ccctgctgct gatgtggaat ataacggtgt
     5221 aacttaccaa atcggtcaag caaataatgc gttgatgtac ccaggtttag ggcttggttt
     5281 gattgcttca acagctacac gagtaaatgc tgaaatcatt tctcaagcta gtcgagcatt
     5341 aggcggaatc gttgatgtaa caaaaccagg agcagctatc ttgcctccag tagcaaaaat
     5401 cactgaattc tctcaaacga ttgctgaaac tgtcgcaaaa tcagttgttg cacaaaaatt
     5461 gaatcgtgaa gaaatcacgg atatcaaaga agctgttgaa tctgctaaat gggttcctga
     5521 atataaatct ttagaagatt aagattgtta tagtaaaaga acatacagaa actttgtatg
     5581 acgtctaaaa agaaaaaagc gactgcggca aaacagcagc cagccaacta ctgctctttt
     5641 gccgcttctc tttggctttt ttcatacgat acagaataag aaaggatctg taaaaatggc
     5701 acaaaaaaca acaaccgcaa ctgcagttga ggaaaagaaa aaagaagggt tttggtcaac
     5761 aaaaattacc ggcgtaagtt tgccccttta tttgatcatg gtccttgtcc tgatcattgc
     5821 aatggcttta ggaaaattac cgacgaatat gattggccca atcgctgttt tggtcatttt
     5881 cggtaattta tttcatttta tcggaacaaa aatccctata gtcaaaagtt atttaggtgg
     5941 tggctccgtt tttgcgatct ttgcctctgc cgcgattgct acatttggga tcttacctga
     6001 gtatgtagta aaatccacag aaaactttgt cagcaatatg ggctttttag acttttatat
     6061 tgctgccttg attactggta gcatccttgg tatgaaccgt aatctattga tgaaagcttc
     6121 tgttcgtttt attcccgttg cacttatttc aatggtcgtc tccttttttg ccgtaggact
     6181 cgtaggtatg cttatcggaa atggttttgc aaattccgtt ttatatatct cattgcccgc
     6241 aatggctggt ggtgttggag ccggagctgt acctttatca acgatttatg caaatgtttt
     6301 aggtacggac gcttcttcta tcatttcccg cctgattcct gcgacagcaa tgacaaatgt
     6361 cctagctatc attggtgcag cgttagttgc tcgtgcggga caatcaatgc caaaattcaa
     6421 cggtaatggt aaattaatgg aaaaaggcga ccttggcgat ggaaaaccta gaaatgtaaa
     6481 accttccatt ccacaattag gtgtcggttt gatggtatca ttgactttct tcatcttagg
     6541 acaaatctgt aactattttg ttcctaaagt ccatgcttac gcattcatga tcattatcgt
     6601 tgttatctgt aaaatcgcaa acctattacc agaatattac gaagatgcag ctatcatgtt
     6661 caacaacttg attgtcaaaa acttgacagc agctgttctt gccggtatcg gtattgcgtt
     6721 attgaatcta aatgtattgg cttctgcttt gacatggcaa tttgttgtct tatgtttgac
     6781 aagtgttatc gcgatttcag tagtttcagg atttgtcggt cgtttgtttg gtttatatcc
     6841 aatcgaatca actattactg ctggcttatg caacaactcg atgggtggta caggaaatgt
     6901 agctgtctta tctgcagcta atcggatgga acttattgct tttgctcaaa tgggcaatcg
     6961 gcttggtggt gccatcgttt tgatcatctc aggtttcttg atgcaattac tttcttagtc
     7021 aaaaaaaata cctgatcatc aagtatcggc ttgatgacca ggtatttttt gataaaaatt
     7081 tacaggattt tcaaaaatct tttttgcatt ttatcggaat gataaccgat gcgttcataa
     7141 aatcggtgtg cttctactct gtattctgca gaattaagtc ttattgctga tattcctcgt
     7201 cttttcgctt cttctacgcc agtcatcaag gcctttccga ttcctctttt ttcagcatgt
     7261 ttagacacag caagtgccat aatattgaac atcggatcgc tgaaaatcga ttcataggac
     7321 tccgcttgta catagccagc tgctttttta gaagtaggat cttcatagac caagaaaaaa
     7381 tttttttctg gctccttcaa cagtttcttc atttgtctca ccgtaacggt taatggtaca
     7441 tcatatccta attgttccgc attgatttct cgtagtgctt ctgcatctcc taaacgcatt
     7501 tcccgcaaca tccaaatcac cagccaattt ttattttttt aaatgaattt ttttatcaca
     7561 gtatactcct tttttcactt aaaaaaagac ttttcagtga ttttgacttg cttgaatcca
     7621 gatccttcta atgtttgcaa gatgacgaaa gcattgtaaa gattgatcaa aagaaccacg
     7681 atgacaatcg cttgaaaaat aaaggtcact ttttttggag acaaaaccct gctaaatctt
     7741 gctccccaca aacctccaat aacagcagcg gggataacat aaaacaacat agataaatca
     7801 aagcgcataa agctacttgt taaagcaatc gttaccaatt ttgccagttg agaaaagaaa
     7861 atcgtagata gagaatatag tgttgcttct tttattggca atgcaaacat cagcatcaac
     7921 aaagaaacat tgactggtcc accaccaata cctaaaaaac tagctaaaaa gcccagaacc
     7981 cccccacata taaggtacca agtccatgac gtcaaatgaa attttggctg atgatgcttg
     8041 gtgtagaaga acgcaaaaac caaggtcaat accgtcaaaa aaatttggat cagctgcaca
     8101 tgcttctcgt tttcaaataa ctgtaaaaaa acttcgaata caatgttgcc gactactcct
     8161 ccaacaacag ctccactaga tacccaaccg acaatctgcc aattcagtga tttcccgctt
     8221 gatacttgtc gcacagtcga aacaatggac atcgtgaata ccgcaactgt tgagtaaaaa
     8281 gaaatccctg caacagaatg ggcgccgata agatcaagaa tcggtttgat caacacgcct
     8341 ccacccatcc cggaaatagc gccaactgtg ttcgccagaa cgatcaccac aaaataaatc
     8401 agaccaatca tcttgtattt cctttctctt gtttatatct tttagcgatt gttactcttt
     8461 tcttttatta tacagtaaaa gaaacaaacg aggcttcgaa agcttttcta ctttttaagg
     8521 aaatttagtg ctaaacgaaa aaaccgggct ctacttttca gagagtccag cttttcttac
     8581 accttatcga ttcttttgtt ctattcttct tccgccatga atgtattgaa tacttcttcg
     8641 atcatatccc attctgcgtc tgtttcgatt tgttctaact cgccttctgt tccatcttcg
     8701 ttttcgatgt atgcataagc ttgtaattct acatcttcat cttctgaaac accagctgga
     8761 tataaaagaa catagttacg tccaaattct tcttgtccgt caactgttag caagatttcg
     8821 tataatgttt catttccttg gtcatccacc aatgtgatgt gttcgtgtcc ttcgtgttcg
     8881 tgggaatggt tatgttctgt cataagatta cctcttttct ttttattatt aaaataattg
     8941 cttacctgag ccgtctaaat aattttgtaa aatcatcaca gctgctaatt tatcgatgac
     9001 tttttttcgt ttcgctcttg aagtatttgc ctgctctact agcatacgtt ctgcctgaac
     9061 agtggttagt cgttcatctt gataatcaac cggcaaggag aatagttctt tgatcttctc
     9121 tccatatgac atggaagctt ctgcccttgg tccgatggaa ttgttcatat tcttaggtaa
     9181 tccaactaca aaacggtcta cttcgtactg cgccactaat tccttcaacc gatcaaaacc
     9241 aaactcttct tctgcctcat tgattcgaat gatttctact ccttgagctg tccagccaaa
     9301 gggatcgctc acagcaacgc ctactgtctt agaaccgaca tccaagccca ttactctcat
     9361 aaattcactc catgatttga gaggtagtct ttgacaatta cttccatgat ttcatcgcgt
     9421 tcgtgacggc gaatcaaatt tcgtgcatca cgataacgag gaatgtaggc cgagtctcca
     9481 gaaagcaagt agccgacaat ctgattgatt ggattgtagc ctttttcttc caaagcccga
     9541 taaacaatcg ctaatgtttc gctcacttct tttttgcgac tatcgtcaaa accaaaacgt
     9601 acggtttcat ctgtaaaacc catgattcta cacctctttc ccgcaaggta attccagctt
     9661 accttctaca cccttatttt actcgaagaa agggaaaact acaaacaaat attccaaact
     9721 tctgaataaa ttaagaaaca cgatttgtct gatttttcct tttttctgtg gctttttcaa
     9781 aagccctctt tccataaagt atacgctata tttctttgaa agtatgtttt tacacaattt
     9841 tttcataaag ttcataaaaa attcgaattg ctgctactaa tttttcgttt tcgttgcttg
     9901 ccctgtctca taattgatgg tgtagccatc ttgaacattg tagataaaga cattgaattc
     9961 aatttttttg tcttcaatgc tttctgcttc caactgcaca cctcttgcta ccagttcatt
    10021 tccctcgaaa tgaggagtga cacgatagcg tacatgatga tttgtttcct tgatatatgc
    10081 cgcaatatca ttttcgtggg caagcatgta cggagcattc aacgaacgtg tccctgtcat
    10141 taagttttta atgttattat tttgaccagt caactggaaa ccaatcaaat ggctgcggtt
    10201 gtataaccat tgtccgtcac ttaatttctt ttgtttccaa cctgtcggat caatatacaa
    10261 aggttctctc ttttcagtag gaaacaagtc ttttcccaac atcgcattgg cttgtcctac
    10321 tctgttcaat ttgtctatat ttgaatagct ttcccatgtc cctttggaaa gactaagatc
    10381 ctcttttgta aactccggct gattcccatt gatttctatt tcttgatgtt ttccatcata
    10441 ttctggcaca tccgctactt gagaagcttg cggtgtggaa tcaaaaatgg ttgtaatttg
    10501 ttggaagata tctgaatctt tttgtgtggt ggaaataccg agtgcccctg caatcacagc
    10561 agcgacgatc cccacgatcg ccttggttgt ttttttgctc atttctttac tcctttacta
    10621 acagttccct aaatcttaac atatgttccc ctataaataa atactggaaa gaaatagaaa
    10681 cagtaaaaaa ataaaaccag tttttagacg gcttaagaat acaaaaaaag agacggattc
    10741 atcgtctcta ctcaatcaca aaacactatt tacaaggtat ttattatcat tcaaaaaatt
    10801 tttcttaata ctcaaaacta aacggcttct gtacgacctc ttggtaaaca gtgttcaaaa
    10861 gcttgcatct gcggtaataa gtccaaccaa gtcaaaaaca taagaagctt ttttgcttga
    10921 ctattcttat tacttgatgc agggcagctt tttcacagcc tcttttaagc gggtgacggg
    10981 aatcgaaccc gcgacatcag cttgggaagc tgggatttta ccactaaact acacccgcga
    11041 tacaaaaaca gtatacgcct ctttcactcc ttcctcaagt ttttttattc tttacgcttc
    11101 cgatatgttt tgtagtaaaa taaaactaat caatcaatta ttggagaaga tgctatgacg
    11161 ggaaaagaat tagatctgtt gctggacact tgtttactcg ctgggaaaat catgatggaa
    11221 agcaatgcgg agatgtatcg ggtagaagat acgatgagtc gaattgcttt agcttctgga
    11281 aatttccgtt tggtcagcta tgtcactcaa accggtctct ttattggtct tgatcgtacc
    11341 tcgaccatcc gaatggaaca aattaccaac cgttcgatta atttagaaaa agtagtaaaa
    11401 gtaaatgatc tttcacgtaa atatgttgct tcagagctaa cattagaaga actttactgc
    11461 gaactgcaag caatcgaccg agaccgttct ttctttcctg tctggttgca aatcgttagc
    11521 gcggcagtga tcagtggaac aattatggtg ttatttggcg gaaagatgac cgatttacct
    11581 gtgactttag tcattggagg attgggatac gcagcttatc tatatagttt gaaatttttt
    11641 agaatcaaat ttttatcgga atttttatct tctttattga ttggcatagc cgctatctac
    11701 agcgtacgtt ttggcttagg aagcaatcag gatctaatca ttatcggatg tgtgatgcct
    11761 ttagttcctg gagttcaaat tacgaacgct atccgtgatt tgctagctgg acattatgtc
    11821 tcaggagttt ctcgcggaac agaagcgatg atgactgcta cgatgatcgg ttttgcgatt
    11881 gcttttattt tccagctttt ttattaggaa aggaacacaa gtatggggac gatatttatt
    11941 cagttttctt ttagtttttt agcgactgcg gcatttgccg ttattacaaa tgtacctagg
    12001 cgttcattga tttgttgtgg tctttctggt gcttttggct ggatgatcta ctggatctgt
    12061 gttcaaatag gcggatctgc tgcttttggt tcattgcttg gttctttagg agtagcattc
    12121 atcagcgatc ttttctctaa acgtttgaaa atgcccgtaa cgattttcaa tattcccggg
    12181 atggttcctc tagtccctgg tggtttagct tatcaagcgg ttcgaaatct cgttactgaa
    12241 tcctatcgag aagcggctca ttatgcggtc caagcaatta tgattgctgg agcaatcgct
    12301 ttaggtcttg tattatcaga agtctttaac cataacatcc gtaatttcaa agtaaagcgt
    12361 gacgcaatca tgatgaaacg aaaaaaacgg ctttttaaat aaacgaagtg taacaaagaa
    12421 aaggttgtga aagagacgtt cagccttgag cgatgacaaa atatctcaca aacattgcga
    12481 caagtaaccg caggagcaat aagaagtaat tatgagtatt aaaaaaagtt ttgtaaaaat
    12541 agcttctcac atttttacat aaaattgact taatactgag aaactgactt ttgaacaccc
    12601 tctatctgta aaaaaggggc tgcgacatga tttttgtcac ggcccctctt ctttattcaa
    12661 attttttcca aaacgttcgt tgataataaa cgatccatag agcggaaagt attagaacta
    12721 aaccgatcac aggaatattc actaaccaaa atggataaaa caaagccaac attccgtata
    12781 agacgataag aataatactg atgatcaatg aacccatcat agttgcgact aaacccaaac
    12841 ttcctgaacc tctgttaaat aattggttaa tattggtcca gttcagcagc agcaaccgat
    12901 aatctctagc gaaataacgt agtgacaata agtagcagcc gaataaagaa ccaaatagca
    12961 agctagggat aaaaaacaaa ggcagctgaa aagacagtcc gcttaataat gcgatcccac
    13021 cagttaaaaa actctgcaat atccatccga atctgaactt ttcttttaaa tattggttca
    13081 tagagattgg caaagaacgg ataaacagaa aattttcttg atccaaagaa ataagattgc
    13141 tgataaaaga agtctgatta actgttaaga aagccaatgc gatccctacc aggaaaacaa
    13201 caccgattaa cttattatct aaatcagtta attttatttc gcctcctagg gcaaaagcaa
    13261 gaataaaaat gattggagtt aacaaggaag aagaaagaac ttgcataatc aaattaggtt
    13321 cttttactaa ttgagcattg taactaaata aaagctgatg cacattttgg ttctttttat
    13381 gggttctttt tattttgaac tcactgtttg aatcagtcag ttgctctgta agctttggta
    13441 ctatcagctg ttttacagca aggaaaaatc ccagagccgt gatagctagt attccccaac
    13501 ccaataaacc ttgttgagaa agtggctgac tggcagcatc atagaatggc aataatatcg
    13561 aaatcggatg ccgatctgtt aactcatttt caatagtccc cgtttgatga ttcatccaca
    13621 tgattccagc aaatgcgatc agcatcgagc tgccaagcaa gatactagaa actgtttttc
    13681 tatgtttttt aaacactgct gttcgagcca aaccaaaaac aatcaaacta gccagacaaa
    13741 aaagaataat caaaaataga gaaaacaaaa ggattgagat aatgatagta agcaaaatcc
    13801 ctagatcagc tcgccatccg gttaaaataa acatgactag aagcggaaaa acaaaaggaa
    13861 tgatggtcaa agcaactacc aaaatctttg ctgaaaaaac ctgttcttgt ccaaaaggca
    13921 gaggcaaaaa tacttgcagg tcatttcctt caaaaaagat attataaatg actgatatac
    13981 tttgagttaa acctaacata ccaaaaagtg ccacgtaata agtaaagaaa cctggcaatt
    14041 taacaaagtt catagcaaac atagcaaatc cgtaaatcag taaaaaaatc agccctgaaa
    14101 aaagattttg agacaaaaga tagatagtta atcgctttcc cttttttcct ttctctcttg
    14161 ctttattcgt gagctgtgga ttaacatata aaagattaac tcgaattaat tctaataatt
    14221 gtcgcttata catacggtcc atctcctgtc gcttgccttc cagccatttt caaataaatc
    14281 ttttctagcg tttcatcagg tgagccattc aataattcac taactgcccc ctgataaatc
    14341 aattcacctt tcttcaaaat agccagttta tcgcataact gttgtgcggt atccaataca
    14401 tgtgtagaaa aaatgactgt ttttcctttt gcggcatgag ccttcatcat ttctttcaaa
    14461 tcaaatgctg cttgaggatc aagtccttgc aaaggttcat ccaataccca aatatctgga
    14521 tctggcagca acgtgccgat caaaatcgtt ttttgacgca ttccatgtga aaaacttgcg
    14581 attgtttcat tttgatgact atacatatca aaaagcgctg tcaattctgc taaacgcttt
    14641 tctttttttt gtgtgtctaa ttcataggca gctgaaatca agtcccaata ttctccagcc
    14701 gtcaattgta aaaaaatatc tggggaatca ggcacataac cgattttttg tttgatactc
    14761 aatcgatttt ccgtcaattt cattccatct actaaaatcg ttcccgatga tggttcaata
    14821 atactaacta aacttttaat cgtagttgat tttccggcac cattatgtcc taaaaatcca
    14881 aaaatttccc cttgtcttat cgagagagac aagtctttta atgcttgttt tttcccgtaa
    14941 cttttactta cattgatgaa ttcaatcatt tctgtactcc ttttgtttgt tattactttt
    15001 tattgtaccc ttcgttattg gtggagtaaa tgtcaaaaag agtgcataac aaaaaaggat
    15061 agaaacgact aggctgctac ttgggttgtt ctcttctaca ggaaaacact cttctaacag
    15121 gtaagatatg agtctttgtt ttctttaaaa tgattcattt tctatctttt cttgcat
//
LOCUS       DACDEG010000058        15140 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.060, whole genome shotgun sequence.
ACCESSION   DACDEG010000058 DACDEG010000000
VERSION     DACDEG010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.060"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IV142_001839"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IV142_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP8485494.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="IV142_001840"
     CDS             complement(322..891)
                     /locus_tag="IV142_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP8485495.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="IV142_001841"
     CDS             1077..1979
                     /locus_tag="IV142_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8485496.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IV142_001842"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IV142_001842"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP8485497.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IV142_001843"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IV142_001843"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP8485498.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IV142_001844"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IV142_001844"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP8485499.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="IV142_001845"
     CDS             8547..8732
                     /locus_tag="IV142_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP8485500.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="IV142_001846"
     CDS             complement(8794..9990)
                     /locus_tag="IV142_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8485501.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="IV142_001847"
     CDS             complement(10160..11596)
                     /locus_tag="IV142_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP8485502.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="IV142_001848"
     CDS             11889..12698
                     /locus_tag="IV142_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8485503.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(12884..15037)
                     /locus_tag="IV142_001849"
     CDS             complement(12884..15037)
                     /locus_tag="IV142_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP8485504.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT         4428 a         3207 c         2674 g         4831 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gacaattttt gtcacaacct tttttgccga gtaaacggtg ttcaaaagcc
    12781 agtttctcgg tatgaagtca aatttaagca aaaatgtgag aagctatttt cacaaaattt
    12841 ttcttcatac tcgaaactaa gcggcttttt cacgacctct tttttatttt gccatgaaca
    12901 tatcataata cgcgtccatt atttctttag caatcgtcaa gtttatgtgg tcttgttcgt
    12961 ctaataagtt cggcaagaca acactgatgg caatttccgg attgtctgtt ggtccgtatg
    13021 ccacgatatt actattgact gtcgcaatat ctggatgacc ttccgcaacc gtttctgcag
    13081 tccctgtttt tgcggaaagg tccattttcg cactggctaa tggtttggct gttgtgtaag
    13141 gatcggtacc atggacaact tggtggaatc cttcacggag gatcgccata ttttctgcag
    13201 aaatattgac cgtattttct actgttgtgc caatttcttt cttcacttca cccaagtttc
    13261 cattttcgtc attgccatag atcgctttga ctaggtgcgg ctgtactcgt ttcccgccat
    13321 tagcgactgt tgaagcatat tgagctaatt ggattggtgt atatgtgtca aactgtccaa
    13381 atgccaaatc ggtaaagtta cctggagcat accattcttt gccattatcc gcattgaact
    13441 tcttcatata atcaacagaa cgtgagatcc caggagattc attcggcaaa tcgatcccag
    13501 tcgttgtacc aagaccaaac tctttgaaag agtctctcaa ttgctgataa gctttagctt
    13561 cttcatctaa actaggtagt cccattccag gtgtatagtc tagtcctaac atctttaacg
    13621 ccactttgat catatacgta ttagaagaca attccgatgc tttgactgca tcaagagcaa
    13681 cttgtccgct tcgattaaag acagagcttt tttctgaagc accttgcaga cgaatcggct
    13741 catcaatcaa aacttgattt ccgctgatcg cgttacttgc ccagcctgct gtcaaagtac
    13801 cagccttgac tacagaccct ggatcaaagg cgctagtaat cgtgcctaaa gcattctcgg
    13861 tgatttcttt ggaaccttgt tcatgactga aaccagtcat tgctagcact tctcctgtct
    13921 gaggattcat agccactgca taagcaccag gcgaatactg tgctttgccg ttgttgatca
    13981 atgtttgata attccttttc aaaatttctt ctacttttga ttggaattct gcattcatag
    14041 ataacattaa attagaacct ttttccccag agaagatttc tttttgattc gaaacatttc
    14101 cttcattatc aagggaaact tcatattggg ttttggttcc ttgcaggaca tcttcatatt
    14161 gtttttccaa ataactttgt cctacacggt cattccgaga ataacctttt gctaggtatt
    14221 tctctgcttc atctgctgga agtccttgct tttcagtcgt cacactgcca agaatacttt
    14281 tcagtgaact tgctgcatta tattctcgtg tccagtctgt gccagtcgaa actcctggaa
    14341 gttcagaagc gcgttctgcc acaacagcta actcttgatc agtaacacca gagtttttga
    14401 tatagacagt gtttaattca tatgcactat tcatccgttt aaagatggta gcaattttta
    14461 attgctcctc gtttaaatta atctcatcag ctgtgacttt attgaccatc gcactatatt
    14521 gttcgctgtt ccctaattgc ttttcttttg ccgaaagtct ttttgtggct tctttcaaat
    14581 gtttgggatc agccagccaa aaatcttttt tatctctatc tgtcagattt tcatctaccg
    14641 gaacatcgat cagcgagttc agcttcgttg cgatttcaag caaatcttta gccgtaactt
    14701 gtgttcctct cgtatatgtg atggcctgat tcgctttatt cgtaactaaa gcttttcctg
    14761 tggagtcgta gatcattccg cgaggagtac tttcttggac cgtaatcgta gaagtagact
    14821 tcactcgcgc ctccatctct tcgccgttca cgatctgcaa ataacccaat ctagcaatca
    14881 acgcaacaaa taacgtaaat ataatgaaaa aaagaaaatt taagcgaaaa ggaacatggg
    14941 accggcgaaa cgttttgttc ttaggttctt gtgcaccttt tcttattttt tctaaccaag
    15001 aagtatttaa tatttttttc ataaaatctt tcatcatttc ctatccttcc gcctatttac
    15061 attcatgctt cattgtaccc aaaaacttct taataaaaaa gaaaaaatgg gcggagcaag
    15121 aaattcttta cctaatttca
//
LOCUS       DACDEG010000059        14743 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.061, whole genome shotgun sequence.
ACCESSION   DACDEG010000059 DACDEG010000000
VERSION     DACDEG010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.061"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            48..1787
                     /gene="yfcC"
                     /locus_tag="IV142_001850"
     CDS             48..1787
                     /gene="yfcC"
                     /locus_tag="IV142_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP8485505.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1944..3128
                     /gene="iadA"
                     /locus_tag="IV142_001851"
     CDS             1944..3128
                     /gene="iadA"
                     /locus_tag="IV142_001851"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP8485506.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3239..3442
                     /locus_tag="IV142_001852"
     CDS             3239..3442
                     /locus_tag="IV142_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP8485507.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3543..4205)
                     /locus_tag="IV142_001853"
     CDS             complement(3543..4205)
                     /locus_tag="IV142_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8485508.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4218..5720)
                     /locus_tag="IV142_001854"
     CDS             complement(4218..5720)
                     /locus_tag="IV142_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8485509.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6268..7947
                     /locus_tag="IV142_001855"
     CDS             6268..7947
                     /locus_tag="IV142_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP8485510.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8074..8553
                     /locus_tag="IV142_001856"
     CDS             8074..8553
                     /locus_tag="IV142_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP8485511.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8772..9452
                     /locus_tag="IV142_001857"
     CDS             8772..9452
                     /locus_tag="IV142_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP8485512.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLIDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9460..10791
                     /locus_tag="IV142_001858"
     CDS             9460..10791
                     /locus_tag="IV142_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8485513.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10806..11768
                     /locus_tag="IV142_001859"
     CDS             10806..11768
                     /locus_tag="IV142_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP8485514.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11879..12217
                     /locus_tag="IV142_001860"
     CDS             11879..12217
                     /locus_tag="IV142_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP8485515.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12296..13003
                     /locus_tag="IV142_001861"
     CDS             12296..13003
                     /locus_tag="IV142_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP8485516.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13106..14587)
                     /locus_tag="IV142_001862"
     CDS             complement(13106..14587)
                     /locus_tag="IV142_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8485517.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4669 a         2541 c         3163 g         4370 t
ORIGIN      
        1 attttttatt catcaaaatt actgaaaaaa gaaaggaaga aataaatttg aagaaaacaa
       61 attcttcagg aaaaccgaaa cgcgacttca ctaaattatc gacaccccat acgtatgtca
      121 ttatttttgg tgtagtcatc tttgcatgga tactgacttt tgtcgttcca gcaggaaagt
      181 tcagcacgca agatattgaa tacaaagatg caaatggcga aacaagtact cgtactgttt
      241 tacgtcaaga ttccttccgt tatgcttacg agctggataa gtcgtatgtg tttgatcaat
      301 tagaagaact gcaagatcat ccagcagaac gtgaaaaact ggatgttccg gaaaaaggat
      361 tggaaaaagt cattgctgac ggagaaaaaa atcttacgca ggaaaaacta gatgaaatct
      421 ccttgacaga tgatgtgctt tacgatcagt acggcgaaaa tatctatgat acttctaaaa
      481 aattgcataa aacagccaaa atatggggaa cagatgattt tggcggcttt ggtttcttaa
      541 attttgtttt tgaaggacta gtatctggag ataagtatgg atctgctgta gggatcgctg
      601 cattgatcct tgttgttgga ggtgcttttg gtattattat gcgtaccgga gcgatcgacg
      661 caggtatcta tgcgtttatc agtaaaacta agggactgga gcgtttagct ttaccgctat
      721 tattctttgc tttttcattc ggtggcgcta cattcgggat ggcagaagag gtaattccat
      781 tctcaatggt gatggttcca tttgtcattg cgttaggata tgactcgatc gtagcagtaa
      841 ccgtgactta tgtcgcatct caagtcggaa atgcgacctc ttggatgagc ccgtttagcg
      901 tagcggtggc tcaagggatt gctggaatcc ccgtgttgtc tggtgcgaca ttccgattga
      961 ttatgtgggt agttgttact gcattagccg ctggttatat gatgatatat gcagaaaaaa
     1021 tccgtaaaaa accagaaagt tcgttgactt acaaatcaga tgattatttc cgttctcata
     1081 tcaaaaagac atctgatgaa aataaaaaat tcatgcttgg gcataaattg atcttattag
     1141 agatgttagt tgtccttgtc tggatcgttt ggggcgtgac tcaaaaaggc tattacattc
     1201 cagaaatcgc ttctcaattc tttgtgatgg gattagttgc tggaatcatt gctgttcttt
     1261 tcaaattaga tggaatgaaa gtcaatgaca tcgcttcttc tttccaatct ggtgcagctg
     1321 atttagctgg tacagcaatc gtagtaggta tggccaaagg gattcttctt gtcttgggag
     1381 gatctgatgc atctgttcct tcagctttga atacgatttt gcatgggatc gggacggcgt
     1441 tgaccggtgt tccagctgtc attggtgctt gggcgatgta catcttccaa agtttgttca
     1501 acttggttgt tacatcaaat tcaggacaag cagctttgac tatgccaatc atggctccac
     1561 tggcagattt agtaggtgta tccagacaag tagcagttct tgcttatcag cttggttcag
     1621 gttttatgga cgcttttaca cccgtatcag ccagcttgat tggtgtgcta ggtgttgcac
     1681 ggatcgaatg ggcaaaatgg gcaagattcc aaatcaagat gcaaggattc ctgtttgttt
     1741 taggaactat tttcatcatg attgccattg caataggtct gcagtagtac tggagacctg
     1801 taaataaaaa agtaatccaa acagtagata aggactccaa gaaccgtcat tttacggtaa
     1861 aatcaaagga gtaaagatcc tatcgtttgg attatttttt aagatcctat catcagttat
     1921 acttaaagaa agaggaaaaa tgtatgaaaa tcattcgaca aattgaagtg tttgcaccag
     1981 attacttagg aaaaatggat gtactgattg ccggtgggaa gattttggct gtagaagaac
     2041 agctagaagg cggctacgaa ggagtggaag tagaagaact ttcaggtgag ggaaaagtac
     2101 taaccccggg tttcattgac tgtcatttcc atattttagg tggaggagga gaaggcggtt
     2161 atcaaaatcg aacgccagaa gtcacattga gccaattgac tacagctggt gtaacgacag
     2221 ttgtcggttg tttgggaaca gacggagaag gtagagatat gactgcgttg atcagcaaag
     2281 cgaaaggtct ggaagcagaa ggaatctcca cttatgtata cgaaggttcg taccgattgc
     2341 cggtaaaaac agtaacagat tctatcatca aagacttttt gacaattgat aaaattatcg
     2401 gaatcggaga aatcgcagta tccgatcacc gttcttctca gccaagtttt gaagagttcg
     2461 ctcgtgcagt agcagatgct cgtgtaggag gcatgttatc cggaaaagct gggatcatca
     2521 atgtccactt aggcggagga aaaagaaagc tagagctatt gacacgaatc gtagaagaaa
     2581 cagaaattcc gatcacacaa tttttgccta ctcatgcaaa tcgcacacca gaattatttg
     2641 aagcttgcgt ggcatttgcc aaacgtggtg gcacgattga cttcactgct agcgaagatc
     2701 ctgatttttg ggagaagaca gatggtgaag ttcgcttcag caaagcactg aaacgattga
     2761 tcgaggaaga tgtttcttta gataatttca cgatgacctc agatggacaa ggaagcttgc
     2821 cttattttga tgaaaacaat catttcctag ggttaggagt aggaagtgcc aaggcattgc
     2881 tagtagggat caaagaagct gtgcaaaaag aaagtatccc tttggaaatc gccttacgag
     2941 ctataaccag taatccagca cgtatattga aattggataa aaaaggaaaa atcgaaattg
     3001 gtgctgatgc agatctgtgt atcttggata aagaaacgct tgatattgat acagttatcg
     3061 ctaaaggaga aataatggtc caagaaaaag aagtcaaagt atgggggact ttcgaaaaaa
     3121 gtttttaatc aacagctcta actatctttt ttagaaaacg agcgttctgt ttgtctgtat
     3181 ctgttgttga tgttaccctg aaggtagatc aataaacaaa ctaagaggag gaagcacaat
     3241 ggcagattta aaaggacgtt ctaaagatat gaaagataaa gtagtcggtg aaggaaaaga
     3301 agcatacggc aaagcaacag acgacaagag caaagaaaca gaagggaaag cacaatcact
     3361 aggtgctgac atcaaagaaa aagctcgaaa tttaggcgac gaagtcaaag acaagttttc
     3421 tcgaaaagac gaaaaaaaat aaaaaccact tattactaat tgaatcaagc aaataaaaaa
     3481 tccgaaccat atctgcgaga cagatatggt tcggattttt tatttgatca ttatcctgtt
     3541 ttttaaactt cgattttttg aaatttttta ttttttagtt caaagacttc atcacagctt
     3601 tttgctactt caatctcatg agtcacgatg ataatacagc gattttgttc atgggcgatt
     3661 tcttggaaaa gtttgacgat atcttgtgaa gttgtttcat ctagatttcc agtaggctca
     3721 tccgctacga ttagttgatg ttcacagcag atagcacgaa cgatagcgac tcgctgctgc
     3781 tgacctcccg aaagtttggt cacctgtttt tttgctaata tttcatcaat tcctacacga
     3841 gccagtcgat ctaaagcaac ttgtcgataa ttctcttgct tggtcttagt gatttccatt
     3901 gctgctatga cgttgtctag agccgataaa taaggaagca agttgtaagc ttggaaaata
     3961 atcgagacat catttttacg ataacttctt aacccgattt tgttcagtga acgatcttga
     4021 tagtatacct ttccttcttt tggtgcatct agtccagaaa taagcgaaag aaaagtcgtt
     4081 tttccagaac cacttgctcc taaaatagcg tacatttttc cagcttcgaa attcagttct
     4141 acatctttat aaagcgcatc ttgcggatct tgataccaaa atcccaattg ttctgtccgt
     4201 aacatcaaat tcctccttta gtttaatagg atttttcttg ggtttaatct taatatccct
     4261 atagaagcta aaaagacaga aaacagactg attccaaatg ctactccggc taatagtccc
     4321 aattgtgcag gtttgacatt gatttccaat tcgctgactt gatcagaaac agcaaatgga
     4381 ttattcgtgg ttccttgttg accatcgcgt tgaccgcctt gttcttgtct gttacctggc
     4441 atttgtccac cgccaggagc ttgctggttt tgtgtcgtgg tttctgtctg ttggtcaagt
     4501 agctgctgtc ccacggcatt cgcgacgacg tttccgctaa gcgaagcaac tcctaatgca
     4561 agaatcatac aaatagccac ttcggtaaac aattggagaa tgattttact tctggattct
     4621 cctaaagata gcaagacgcc taactcatgc cgacgctccc gaatggtaat cataatgatc
     4681 agagtaagta tgataatacc ggctacagcc actagtaaaa caacattttt agaaaaactt
     4741 gccacgttgt ttaaaggttc caacattgac tgatacatgg aatcatttga ttgtaagcta
     4801 aatgtatctg tgtcgatcag cttctcagct tctttgagaa actcatccat tttatctgga
     4861 tcagatagag tgtaaatagc agaatctatc gtgttatcgt cagatgtccc atttagttca
     4921 tttgcaaaag tgtaagaagt gtaaagtgta ttcgacggat tcatgaagtt gaatttcata
     4981 cccatactgc cgcttgtttc gctggattca taaattcctt tgatcgtcat ttcataagtg
     5041 gtatcttcat cttcagtact cgtgattgtg aaagtatctc cgactgccag gtcatttgtt
     5101 tctgctagag tagaatcaat gactacattg tttgtgcctt tgtcgctgtc atctattcct
     5161 tcaccatccg tgatttttgc tgttccatca gaaaaattac tattttgtgc agttgctgac
     5221 acaccactga tttgaaagtc tgcttgagtc atttgcggat tcccgcccat tcctccagca
     5281 ggttcaccgc ctgcatcagt agtttctgaa ctgtcttcac ttgaatcaga actagtgatg
     5341 gcagttattc catcttttgc catagctgag gcggaacttt caaaggaata actctttaca
     5401 ttatctaatt cagctatttt ttccgcgtcc gttaaggaaa ctggtgtaag agaaaaactg
     5461 cctggatctg gacgttcttc agtggtagaa ccgcttgaag aagtttgttc ctgtttttta
     5521 aatgaagctt ctcgattggc acttaaagtc actgttgctc caacttcttt ttgtgcttga
     5581 ttcgctgctt cttctgcagc gcttcgaatc gtgaggccag ctagcacaaa aataagaatg
     5641 gctgtgaaca cggcaaacaa taacaatgaa cgtccccatt ttacttttgt acttttccat
     5701 gcacgtttca taaaattcat attttgcatc cctcctagta gatgcatttg ataatagaca
     5761 acctatctta aagctagctt aaatcttatg ctgtttgcac accatcaaca tgaggttctg
     5821 tatatataaa aagtcactat agttattgca aaaaatgtgg taaaatgaaa aaagtatgtt
     5881 tcaaaccgac gaagaagaaa tgaaagagga atagcaaaag aaatcctaga tgctacagag
     5941 ggatttttac gtgcatagtt gttctaacaa ttgctgatac tagcgattta gtttatttgt
     6001 tgtataataa tggggaacag ctgaatactt atataagtat tctggctata gcgtttcgca
     6061 aaagatccat tttgttaatg aagtatatcc aaaataaaat gcccatccaa ctgtcagaac
     6121 gcggatgtgc tggtaatttt cctgctgatt ctccataagt tgagaaatag tgattttgca
     6181 tatgcttcat tggaatggct catatattta gataaagaag aaaatcgaca tctttaaact
     6241 tggatgtcat aagaagaggt gaagtttgtg agtaccatta aaattatccc attaggcggc
     6301 gttcgtgaaa atggaaaaaa catgtatgtc gcagaagtgg gcgaggatat ttttatttta
     6361 gattgtgggc taaaatatcc tgaaaatgag ttgttgggga tcgacgtcgt aatccctgat
     6421 tttacgtatt tagaagaaaa cattgatcgt gtagctggtg tctttttaac acatggacat
     6481 gcagatgcga tcggggcact tccttacttg ttgtcaaaaa tttctgtacc agtattcggg
     6541 acagagttga cgatcgagtt agcaaaattg aatgtcagtc gtaatgaagc agccaaagga
     6601 tttaaggatt tccatgtggt agatgagcat acagaaatag actttggtga aacagtagtg
     6661 agctttttcc gtaccactca tacgatacca gattcagtgg ggatcaatct gaaaacaaaa
     6721 gaaggtagta tcgtttatac gggagatttt aaatttgacc aaacggctat cccaatgtat
     6781 caaacagatt atgcacgatt ggcggaaatc ggcaaagaag gagtcttagc cttactaagt
     6841 gaatcggcaa atgcagaaaa tcctcagcct gtagcttctg aagcccaaat agcagatgaa
     6901 gtatttgata cgatcaaata ttgggaaggg cgtatcatcg tcgcttgtgt agcaagtaat
     6961 ttgcaacgag tgcaacaagt attggatgca gcagaaaaag cacaacgaaa agttgtcttg
     7021 actggtcaag atttcgaacg aattattcgc acagcgatgc gtttagaaaa acttcaatta
     7081 ccaagtgaag atctgcttgt tacgttgaaa gacatgaaaa actatgatcc agaagaactg
     7141 atcattttag aaactggccg aatgggtgaa cccatcaagt ctctgcaaaa aatggcaaac
     7201 ggtacacacc gtaatgtcaa gattgaagaa ggagatttgg tttatatcac tactacgcca
     7261 agcatcgcga tggaaaccat cgtggcgaaa acggaagata tcatttatcg agctggtgga
     7321 acagtgaagt tgatctctga gaatatgcgc gtttctggcc atgccaatcc taatgacttg
     7381 caattaatga tcaatttgat gaaaccaaca tattttgtgc cagttcaagg agagtatcgt
     7441 gaattagctg ctcatgcaga tttggctcat gcagtaggga tgccatacaa aaatatctat
     7501 attactggac gcggagacgt gctggaatat aagaacaagc gtatgagtgt tgcaggaact
     7561 acaagtgcag aaaatgtaat gattgatgga ttaggcatag gtgatatcgg aaacattgtt
     7621 ttacgtgatc gaaaaatctt gtcagaagac gggattttcg tagcggttgt cactatcagt
     7681 cggcgagaaa agaaaatcat ttcaaaacct caaattactt cacggggatt cgtttacgta
     7741 aaagctagcc gtgatctgat gaaagaaagc agcaatatga ttgaaacaat cgttgagaaa
     7801 catttagaaa gcaatgattt tgagtggagc aaattaaagc aagacatccg agatcaatta
     7861 ggccgttact tatttgaaca aacaaaacgc cgcccagtga tcttgccagt cattatggaa
     7921 gctactcaac gtcgaaataa aaaataaaca aaaagagtaa gatgaacctc tctatagagt
     7981 gctcgtcttg ctcttttttt gcgaaaaaca aataattttt gaatacagca tccctcgctt
     8041 tataataaac gatagaataa agggagtgaa tcaatgatga gtcaaacaaa agtcgaacgt
     8101 tatttagaag aacaaaagat cagctatcaa ccacttgatt ttagtcatgt ggtagaagaa
     8161 tctttcacag aagaactaca aaaaagaggg attgatccag ctttgatatg taaaactctc
     8221 gtagtgaaag gaaacaaaac tggggtgatc atcgcattga ttcctttgaa cgatcactta
     8281 gattacaaaa aaacgagaca agtgacgaaa gatcgtaaag ttggttttcc tggaatggat
     8341 tttgtattaa tgcatacagg atatccccac ggagcaaaca cccctattgg aataaaactc
     8401 gcacatcctg attattgttt tttagcagac agttctttaa aagaaaaaga agaagtaatt
     8461 gtgtcaagcg gtgaaatcgg gagaagcctt cggataaaag tagccgattt agaaaagatc
     8521 gtccatccta tttacgctga tttaattagt taagggacga gaagagaagc taagcagagc
     8581 agagacctca aacttgagat tatatctgct tggtacttgg tgcatcctgt attatctgtt
     8641 acaattgctt tgtacatact gtactatcaa tccaatgaaa atatatttag cagctatatt
     8701 ttttcattga tgtgcaagtg atgaaatcgt ttttaataaa aaagatacag agaaaacgcg
     8761 aggtgtaaaa tatgaaaatt acgtatcatg gacattcctg tttaacaatt gaaatggaaa
     8821 atggccaacg cgtgatgatt gacccgttta ttacaggaaa tccattgata gacttaactg
     8881 cagaagaagt aaagacagat tggttgctga ttactcatgg tcatagtgac cacataggag
     8941 atatgattcc tattgcaaaa gaaaatcaag ccacaattat cagtattgtt gagattgctg
     9001 attatgcgaa cagcagagga gtagattcat tcggtatgaa tatcggtgga aaacatgcgt
     9061 ttccattcgg cgtggtaaaa ttcgtacatg cacagcatag ttcaagctat gaagtagacg
     9121 gtatcgttca gtatatgggc gagcctagcg gaattattat tcaagcagaa ggaaaaacga
     9181 tttatcatgc aggggatact gcttatttca gtgatctagg attgcttgca gaagaattcg
     9241 atattgatgt cgctttcttg ccgattggcg ataactatac gatggggcca gaagatgcag
     9301 caaaggctgc gcaaacaatt cgtgccaaga aagtagttcc aatccattac aatactttcc
     9361 ctgtcattga acaagatccc gaatcttttg tagagttact ggaagatagt gaaggaaaaa
     9421 taatgagcgt ctcggaaacg ctggaagtgt aggcagaaaa tggcgacaaa acatgatttg
     9481 attttagaat atattgaaag tttacctgtt ggcaatcgaa tttctgtaag aagcatcgca
     9541 aaagaattga atgtcagtga aggaacagcg taccgtgcaa tcaaagatgc agaaaatgtc
     9601 ggactagttt caacaattca acgagtaggg accatccgta tcgaacgcaa attaaaaaaa
     9661 catattgaac gtttgacatt tggcgaggtc gtcaggatca ttgaagggga cgttttaggc
     9721 ggggcggcag gattagacaa ggtcttgaat aaattcgtca tcggcgcgat gactaaacat
     9781 gccatggaac gctatattac gccaggatca ctaatgatcg tcggcaaccg ggcggaagtc
     9841 caaaaattag cattggaaga tggcgccgca gtattgatca caggaggatt caatacgact
     9901 gctgagattg caaagctagc tgatgatctt gaaatgccga tcttacgcac ctcttatgat
     9961 acatttacag tcgcaaccat gatcaaccga gcgttaagcg accaattgat caaaaaagat
    10021 atcatgcttg tcagtgatat ctatatgcca gctgaaaaga ctcattattt gcagcagaca
    10081 gatacgattt ctgattatca gcgtttgtca gaagaaacgc agcattcgcg ttttccagtc
    10141 gtcaaccgcc atcatcgttt gatggggatt gttacggcaa aagatgtttt agggaaatcg
    10201 ccaaaccaga tcatcgatcg ggtgatgacg aaagaaccaa tcagtgtaaa gaaaacaatg
    10261 agtattgcct ctgttagcca tcaaatgatc tgggatgggc tagaagtcat gccggtcgtt
    10321 tccgatgact tgacattgga aggcttgatt acccggcaag atgtaatgaa agcgatgcag
    10381 cttgtccagc gacaaccgca gattgcagat acgatttcgg atcagatatc tggaaatatc
    10441 catacgattg atactgaccg tgaaggaaat cgtttggaaa accctaagtt caaattcgta
    10501 gtagcaccac aaatggtcaa tggcgttgga accatttctt ttggcgtact tagcgaaatc
    10561 atttctgatg tcgcacaaaa aacaatggtg atgaaccaaa aacgaaatat cttgattgaa
    10621 caagtgaacc tacattattt gcggctgatc caattggaaa gcgaattaga cattcgtcca
    10681 cgtgtattgg aaatcggcag acgttccgct aaactggata tcgaagtgtt tattgaaaac
    10741 accatcgttt ccaaagcaat cgttgtttgt caagtaatgg aacgttctta gaaagcagga
    10801 gtgaaatgac agtacagcaa gaaatagtag atacaataaa aaaatacgat aaaatcatta
    10861 tccacaggca tttgcgccct gatcctgatg cattaggatc acaagtagga ttagccgaac
    10921 tattacggga aagtttccca gaaaaagaaa tacgtcaagt aggtgaaacg attgaagggc
    10981 ttcgcttttt agcagaaatg caagaagttg aagatgtctt ctaccaagga gcgctagtca
    11041 tcgtgactga tacagctaat tcacccagaa tcagtgatca aagatatcag ttggcagaaa
    11101 aactgatcaa aatcgaccat catcccaatg atgaccctta tggagatctt gtgtgggtaa
    11161 atacaaaagc aagtagctgt agtgagatga ttgcagaatt tgctttgatg tttcctgaat
    11221 ctttaaagat gaacgttaag gctgcacggt tactttatgc aggaatcgtc ggagatacag
    11281 ggcgcttttt gtatccctcc acgacgtccc gtacattaga gattgcttcg atattgagaa
    11341 attatccatt tgatgcaagt gctttgaaca gagagattga acaaatgccg atgaaagtcg
    11401 ctaaactttc cggttatctt tatcaaaata tccaggtgga tgaaaatggt gcaggaagaa
    11461 taatccttcc gcaagaaatt cttgataaat atggaatcga tgattcagag acagcttcga
    11521 tcgtttcact tcctggagtg attgacgaag tgctggcttg ggggattttt gttcagcagc
    11581 cttcagggta ttaccgcgta cgtttacgtt ctaaggggcc gatcatcaat gaattggcta
    11641 aacgccatca tggaggaggc catccattag caagtggtgc gaatgcaaaa gatttagcag
    11701 aagtagagga aatctataaa gagatccaag atatatgcca atcgtataag agcagaaaaa
    11761 cagaatgata cttcgttcga aaacgtgcct tttgtagtaa aaatcacaaa tgtatctttt
    11821 caagatcttc tttttccgtt acaatagtgc tcactaaaga ttttaaagga gaaagaagat
    11881 gacattacca aattgcccag aatgtggctc agagtacaca tatgaagatc gaggcttgtt
    11941 gatttgccca gaatgcggga acgaatggaa caaagaagaa caagaaactg tcatagaaga
    12001 cggattagtg gtattagatg caaatggaaa ccgtctgtta gagggcgata gcgtgacagt
    12061 gattaaagac ttgaaagtaa aaggtgcgtc aggtgccatt aaacaaggaa caaaagtcaa
    12121 gaacatccgt ttagtagaag gcgatcataa tatcgattgc aaggtagaag gatttggacc
    12181 gatgaaactg aaatcggaat ttgtgaaaaa gaattaaaga atattcaagg tcttttcaca
    12241 ttcttttcct actactaatg ataaaataaa gtaaacgaaa aggagtgaaa cgaaaatgta
    12301 tggttatgga atgtatggtt ttttagatcc gacatttctt ttggtcattc ttggattagt
    12361 tatttccgga attgcgtcag cgtacgtcaa tagtactttc cgaaaatacg atcaagtgag
    12421 aagcaaaaac aatgtgactg gtacacaagc agcacaatat atattacaaa gtcaacagat
    12481 aaataatgtc ggtgttcaac aaattgccgg agatctcaca gataactaca attcaggtaa
    12541 taagatgttg agtttgtctc aagcaactgc acagtctact tcagttgctg cgataggtgt
    12601 agcagctcat gaatgcggac atgcggtaca agatgcttcg ggttatgtcc cattgaagat
    12661 tcgtgcagca cttgttccag cagctaactt tggttcgatg atttcgttcc ccttgattat
    12721 ggtaggtgta ttgcttagct ggaactctac gttaatcaat atcgggattt tcgctttttc
    12781 tttagcatta cttttccaat tggtgacatt accagtcgaa tttaacgcgt ctcggcgagc
    12841 aatccaaatt ttgagtgaag gcggactact gacggaagaa gaagtaccga tggcaagaca
    12901 cgtattattt gcagcagcac tgacttatgt tgcagcagcg ctttcgacat tcctgcagct
    12961 gttgcgcttg atcttattgt tcggcggcaa ccgaagagat taaaataaag aagttgtgaa
    13021 atcagcgctt tgatctgagc taataccgag agttagctga ttgaatacca tttatttgga
    13081 ataagaggat gtgacaagaa ttttgtcaca tcctctttgt tttgttctca taaccttttt
    13141 tcatatacag ttcatgaacc gcttttatta acttgccaaa taacactgta ttgtgcgttg
    13201 tatccagata gaaagtctcc gtttcatctg aaattttctc atgctgatct gtaatgatca
    13261 aatcagcatg ttctgaatat gcggtaattt taacagcatc tttgcgaaat aactgctgca
    13321 aatggctttg aagatgataa tttcctgtaa actgtttggt tatctgcaaa tggatgagta
    13381 actgctcttt cgtttctgtt tgtagtaagt gttggatcaa ttgactaagg tattgactga
    13441 tttggtttct ttccccattt tttgaaaaaa ttgtatcgat tttttctctt aatttctttt
    13501 cctgtataaa agaactagaa tgattggacg tggcttctgg aaagcaaagt gtagaaaaac
    13561 tactgctgga ttcacccaac agttcgaaaa aaacaactgc caacacaaga aaataaaaat
    13621 aaattggtat tttattttct aaatggttga gaggaaactg ttgagttaag taagtcatca
    13681 attgaccgac ttgtcgacaa ataaggtttt tgcttgtgga aaataaaaaa ccaatctcct
    13741 gtttttgtcg aaaagagatt tgatttggaa aaaaacaata gaaaaaataa ttaaagtagg
    13801 ctctctctgt ttctgtctct tcacaagggc gactcactga aaaagaagta aagtattctg
    13861 aataatcatg tgtatgctgc aataagtcca tcaattcagt gatttcagaa gaagtttttg
    13921 gaaaatatgc ctgttgtttg tttcgtaata tgaataaagt taacagcaag tagatatttt
    13981 tttcttgttt tgaaattttt tctatttgct catctgtact gttcattttt agtgcattta
    14041 attcaagtga ggaaaatggc caatcgttac ctccaaatgt atctgaaaga aaaaagtaaa
    14101 gatagagacg gatagataac tcatttcctt caagagtaca gcaatttttt acagatagga
    14161 tccgtaaatg gaattttttt aaggaatgat tgagctgttt tgtcagtttt tcgagatagg
    14221 agggcgatat ataaagttgt tcaagtaaat atttttttgg tacagcagtt tgtggataat
    14281 tgattagtaa catcaaatag ttaaaacgat tggattcttt taggtagcat aattttaact
    14341 tagaaaaaac agctaacttt ttagtttgat cggtttcttt ggtttggatc tctcctttat
    14401 gattagaata aataaattca tcaatcaata aaaattgctt gagtgataca tttatttttc
    14461 ggatcagttc tttgattgtc cgttttggaa tatgtaagtc attggataat tcttgtaaag
    14521 aacaattatt tttttgaatg accatttgta gaacaagtaa ctcttttttt cttgcttcaa
    14581 ttagcataaa tacctccttt ttattctgtt tttgtatata gttagacaaa aactgtgagt
    14641 ggaaagtcat ttcagtctgt gttattctca cagagtgtcg ccttcttttt tatataacgt
    14701 tatttaattt ttttgttacc ttgtttattc tattgtttat ttc
//
LOCUS       DACDEG010000060        14652 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.062, whole genome shotgun sequence.
ACCESSION   DACDEG010000060 DACDEG010000000
VERSION     DACDEG010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.062"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="IV142_001863"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="IV142_001863"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP8485518.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="IV142_001864"
     CDS             complement(2260..2511)
                     /locus_tag="IV142_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP8485519.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2657..3280
                     /gene="lexA"
                     /locus_tag="IV142_001865"
     CDS             2657..3280
                     /gene="lexA"
                     /locus_tag="IV142_001865"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP8485520.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3460..3678
                     /locus_tag="IV142_001866"
     CDS             3460..3678
                     /locus_tag="IV142_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485521.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3967..4479)
                     /locus_tag="IV142_001867"
     CDS             complement(3967..4479)
                     /locus_tag="IV142_001867"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP8485522.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4493..6790)
                     /gene="recJ"
                     /locus_tag="IV142_001868"
     CDS             complement(4493..6790)
                     /gene="recJ"
                     /locus_tag="IV142_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP8485523.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6932..7366)
                     /locus_tag="IV142_001869"
     CDS             complement(6932..7366)
                     /locus_tag="IV142_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP8485524.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7376..8164)
                     /locus_tag="IV142_001870"
     CDS             complement(7376..8164)
                     /locus_tag="IV142_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8485525.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8161..9102)
                     /locus_tag="IV142_001871"
     CDS             complement(8161..9102)
                     /locus_tag="IV142_001871"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP8485526.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9279..9866)
                     /locus_tag="IV142_001872"
     CDS             complement(9279..9866)
                     /locus_tag="IV142_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP8485527.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10087..11400)
                     /gene="obgE"
                     /locus_tag="IV142_001873"
     CDS             complement(10087..11400)
                     /gene="obgE"
                     /locus_tag="IV142_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP8485528.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11688..12698
                     /gene="gap"
                     /locus_tag="IV142_001874"
     CDS             11688..12698
                     /gene="gap"
                     /locus_tag="IV142_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP8485529.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12783..13718)
                     /locus_tag="IV142_001875"
     CDS             complement(12783..13718)
                     /locus_tag="IV142_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8485530.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            13904..>14652
                     /locus_tag="IV142_001876"
     CDS             13904..>14652
                     /locus_tag="IV142_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAP8485531.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERPVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTP"
BASE COUNT         4113 a         3247 c         2588 g         4704 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aacaactaga taaactaaat
     2581 aaaatacgaa cattcgtttg atttagcgct ttttatatgg tactatttat ttatcaagtg
     2641 aaagaaggtg taccttgtgg cccgacaaac tgaatcaaga caaatcgaag tcttacgata
     2701 tatccatgaa caagtaaccc aaaaaggata cccgccgact gtccgtgaaa tcggggaagc
     2761 tgttcagctt tcttctactt ctactgttca cggtcatctt gcacggcttg aaaaaaaagg
     2821 gtttatccaa agagatccga cgaaacctcg tgcaatcgag ttgacaaaac aaggtttaga
     2881 aaagattggg attcgtccaa caactattcc agtacttggc gtcgtaacag ctggagaacc
     2941 cattttagca gtagaggatg cttcagactt ctttccaatc cctcctaatc tttcttccga
     3001 agaaggcagt ctctttatgc tgacaattcg tggagatagt atgatcaatg ctgggatatt
     3061 ggatggtgac aacgtgatcg ttcggaagca agaaactgct caaaatggcg atattgtcat
     3121 tgcaatgact gccgaggacg aagcgacttg caaacgattc tataaagaaa aaaacttttt
     3181 ccgtctgcag cctgaaaacg acttgctcga accaatcatc ttagatcaag tatctatatt
     3241 aggacgagta gtaggacttt atcgtagtca tatctattaa ttgctactaa ttaaaagaaa
     3301 actttttaaa aagagtttga gacgttggtc tcaaactctt ttttcgcccg ggttcttctt
     3361 ctttcaaatt tttatcactt ccctctttac aaaccgaaca aatattcgta tactaaatat
     3421 acgaacaatt gttcgcataa attaaagagg tgagaattca tgaaatcaat gagacgtctt
     3481 ggcttagtat taactgtttt gtttttaggt attattattg gtaatttcgg catacgtcca
     3541 gctttacttg tgtttgttcc gttatttact ttgtggttca tgttgtggga tgaaaagaag
     3601 taccgtcaat ctgagcggcg cagaattcat gaagatcatt tctatcatga gccctactac
     3661 agatccaggc aatcataata aggattgact gctaattatt caaaagacaa gaagttaaac
     3721 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
     3781 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
     3841 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
     3901 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
     3961 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
     4021 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
     4081 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
     4141 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
     4201 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
     4261 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
     4321 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
     4381 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
     4441 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
     4501 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
     4561 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
     4621 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
     4681 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     4741 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     4801 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     4861 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     4921 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     4981 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     5041 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     5101 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     5161 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     5221 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     5281 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     5341 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     5401 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     5461 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     5521 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     5581 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     5641 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     5701 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     5761 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     5821 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     5881 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     5941 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     6001 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     6061 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     6121 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     6181 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     6241 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     6301 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     6361 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     6421 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     6481 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     6541 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     6601 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     6661 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     6721 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     6781 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     6841 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     6901 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     6961 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     7021 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     7081 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     7141 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     7201 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     7261 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     7321 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     7381 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     7441 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     7501 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     7561 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     7621 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     7681 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     7741 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     7801 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     7861 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     7921 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     7981 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     8041 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     8101 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     8161 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     8221 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     8281 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     8341 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     8401 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     8461 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     8521 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     8581 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     8641 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     8701 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     8761 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     8821 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     8881 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     8941 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     9001 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     9061 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     9121 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     9181 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     9241 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     9301 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     9361 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     9421 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     9481 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     9541 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     9601 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     9661 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     9721 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     9781 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     9841 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     9901 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     9961 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
    10021 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
    10081 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
    10141 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
    10201 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
    10261 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
    10321 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
    10381 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
    10441 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
    10501 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
    10561 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
    10621 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
    10681 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
    10741 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
    10801 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
    10861 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
    10921 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
    10981 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
    11041 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
    11101 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
    11161 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
    11221 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
    11281 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
    11341 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
    11401 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
    11461 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
    11521 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
    11581 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
    11641 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
    11701 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
    11761 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
    11821 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
    11881 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
    11941 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
    12001 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
    12061 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
    12121 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
    12181 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
    12241 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
    12301 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
    12361 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
    12421 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
    12481 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
    12541 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
    12601 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
    12661 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
    12721 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
    12781 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
    12841 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
    12901 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
    12961 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
    13021 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
    13081 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
    13141 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
    13201 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
    13261 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
    13321 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
    13381 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
    13441 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
    13501 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
    13561 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
    13621 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
    13681 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    13741 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    13801 tataacccgc gaattgtaaa attaagttag aaaaataaaa aggcatttat ggtacactca
    13861 aattgtattt ccgacgaaag aaaacaaagg agtgtaacca taaatgacct acaaacatct
    13921 taccatagac gaactgacaa tgatagaatc atattatctt caacataata agccggttga
    13981 aatcgctaac cgaatgggac gtgctataca aactatttat aatgtagtca ataagttcaa
    14041 gcaaggtaag actgctcttg attattggca ccagtataaa gaaaataaga aaaaatgtgg
    14101 tagaaaagtc attcaattac ctgctcatga agtagattac attaaagaga aagtcactct
    14161 tggttggacg cctgacgtca ttatcgggcg aaaagaaagg cctgtttcat gcggtatgag
    14221 aacactttat cgtttatttt ctaaaggaat atttgatatt gacacactac cgatgaaagg
    14281 taaaagaaaa cccaatggcc atcaggaaaa acggggaaaa caacagtatc agcgctcaat
    14341 ccatgatcga cctgataatt atcctgattt caattctgag tttggtcacc ttgaaggtga
    14401 tacgatcgtt ggcattcatc ataaaagtgc cgtcattact ttagttgaaa gattatctaa
    14461 agtcattatc acgattaaac ccaacggccg taaggcatta gatattgaaa ctgcccttaa
    14521 tcaatggttt tctcgcttcc ctaaaaactt ctttaaatct attacgtttg actgtggaaa
    14581 agaattttct aactggaaag ccattagtaa ccaacatgat attgatatat attttgcgga
    14641 ccctggaaca cc
//
LOCUS       DACDEG010000061        14544 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.063, whole genome shotgun sequence.
ACCESSION   DACDEG010000061 DACDEG010000000
VERSION     DACDEG010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.063"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(195..761)
                     /locus_tag="IV142_001877"
     CDS             complement(195..761)
                     /locus_tag="IV142_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8485532.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(761..1717)
                     /locus_tag="IV142_001878"
     CDS             complement(761..1717)
                     /locus_tag="IV142_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP8485533.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1717..2373)
                     /locus_tag="IV142_001879"
     CDS             complement(1717..2373)
                     /locus_tag="IV142_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP8485534.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2743..4761
                     /locus_tag="IV142_001880"
     CDS             2743..4761
                     /locus_tag="IV142_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8485535.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5188..7602
                     /locus_tag="IV142_001881"
     CDS             5188..7602
                     /locus_tag="IV142_001881"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP8485536.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            7950..9503
                     /locus_tag="IV142_001882"
     CDS             7950..9503
                     /locus_tag="IV142_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP8485537.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(9884..11668)
                     /locus_tag="IV142_001883"
     CDS             complement(9884..11668)
                     /locus_tag="IV142_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8485538.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11683..13404)
                     /locus_tag="IV142_001884"
     CDS             complement(11683..13404)
                     /locus_tag="IV142_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8485539.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13504..14265
                     /locus_tag="IV142_001885"
     CDS             13504..14265
                     /locus_tag="IV142_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8485540.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4716 a         2698 c         2900 g         4230 t
ORIGIN      
        1 aaaaagctgt cctgcatcaa gtaataagaa cagccaatca aaaacagctt ctcatgtttt
       61 tgatttggtt ggacttatta ccgcagatat aagcttttga gcaccgtgaa taaagaggtt
      121 atgaaatcaa cgctctaacc tgagtattag agaaaaaaac gcaaaacata tcatttatgt
      181 tttgcgtttt ttatttatct ttttttctca atacaatata aaataggcgg ctggttcttt
      241 tggttgataa accgataatt cagcacatta tatgtttcct gtggcagttt ggaacaaaaa
      301 gaatcaacca tatccagttc tttttctcca ccttcatgac catagtagac gacaaggatc
      361 aatcgtccgc gaggcacaag tcgtttcaat atttcttcca tagctgtgcg tgtagtctct
      421 ggcatagtga tgattgcttt atcacttttt gggagatacc ctagattgaa aatccctgct
      481 ttgagatttt gttgttcagc tatcacttct cctaacgttt cgtggccttg tagaaacaat
      541 gagacacgtt cttctaactg acgctgttct agcctttcac gagtagcatc gattgcttgt
      601 tgctggatat caaaactata gacatgacca gtttttccga tgtgttctgc tagaaaaacc
      661 gtatcgtggc cattgcccat cgttgcatct acgacgtgat cccctggttg caagatttct
      721 tgcagtaatg tatggctgaa atgcaaagca gtttgcaaca tcagacgctc acctttttcc
      781 gtacatcata ttttccttgg aagctatctc tgcgaagaag ttcttcatca attgcattca
      841 agacttccca ttttttcaag ctccacattg gtccaatcaa ggaatcccac ggcgcatccc
      901 ctgtcaaacg atggatgatg atctcttttg gtatcatttc cagctggtcg caaatgacat
      961 tgacatagtc aggacggctc attaattgaa ggcgaccttc atgatagtcc cgcaacattc
     1021 tcgttttgcg catcagatgc atcaaatgga gcttgattcc ttggatatct gaatctaaaa
     1081 tggtgcgtct gacattttct ttcatcattt ctaaactttc gcctggtaat ccgttgatca
     1141 aatgcgtaca aacacggata ttatgtttgc gtaatttagc cacgccatct aaataagttt
     1201 gataatcatg tgctcggttg atcagttttg aagtttcttc aaaagttgtt tgcaatccaa
     1261 gttctaccca taaatacatc cgctcgttca gttcagcaag gtaatcaact acttcatctg
     1321 gcagacaatc cggtcgtgta ccgactgata atcctacgac acctggtaaa ttgactactt
     1381 gctcaaatcg ttctttgatt atttcaactg gcgcatgggt gtttgtaaaa ttttggaaat
     1441 aaacgatgta ttgttggaca tgaggccatt tttgatgcat catgtcgatt tctttctgga
     1501 attggattgg aagagggtct tcaggtgcaa cgatcatgtc tcccgaacca gatacactgc
     1561 aaaaagtaca accgccatgg gcaacagtcc catctcgatt cggacagtca aatcctccat
     1621 cgatcggcac cttgaaaatt ttcccgccaa actcatttcg taaagcgtaa ttccaagaat
     1681 gatatctttt atttccttct gaataaggaa aagtcattaa aagtccttct ttctgtaaga
     1741 gttatctatt tgttttcttt tttctctata cactggataa gtcaggcaca gccaagaaag
     1801 actgagtaag tagccgccga tgatatcagt aggaaaatga acgcccagat aaatgcggct
     1861 tatcccgaca cttactataa taaacatcat cagcaattga agcaatgtgc gtaaggttct
     1921 tttttgaaca aaagctggaa gcagaaatag taaagaaccg taaagaatca tactgcctgc
     1981 tgcatgtcca ctagggaaac tgtaacttgt ttcaactact aagtgcgcca atgaaggacg
     2041 ctctcgcatg aaaaatagtt tgatcaccgg gttgatgatt ccagcaatta caataaatcc
     2101 agaaacgaac caaaatgttt ctgccctttt tttcttatac cacatccata aagccgcaca
     2161 agcaaataaa atgatgacgg taagaggatt accgaattta gtcataaaga gttggtagct
     2221 gttcagatca ggataagaag ctctgacaat gcctgtaaac cactgatcga aaccagaaag
     2281 ccacgacaga tggaacttta cgatccagcc caaaatgaaa aacaggacaa gaaagaagct
     2341 accagccacc tgaaatgatg ttttatattt catacaattc ctcctttgaa gcaatgactt
     2401 attatagcat ttttggtcca gcgaaaaaaa cggtaaagag ataaaaccaa tgtatgattc
     2461 ctctttgaat aaccaatatt cttataaaaa aaataactgt aatcggaaag acattttttg
     2521 gtaatgcaaa tgaaatgtta gggaaataaa atcagtgttt ttcgaaatcc ttttaaacaa
     2581 gaacgtagac gtaggaagaa cttgaaaatg aacagaagtt gttcattacg gtaaattaca
     2641 atatgattac aaaaaacaac aaagctctta aataactttt aataaatatt aagagaagtt
     2701 ataattatta ataatagata aattgaagtt ggaggataga agatggaaaa cattgcaaga
     2761 aaagaacgga gacgtttaga acagaccaaa cgttttcgaa aagcaaaacg aggggcagca
     2821 atcgtgggaa ctgcgatggt aggctgttct gtcgctgccc cattagtaca gccggttcct
     2881 gttcaagcgg atgaaacacc tgttcagttc ggcgcaagaa tcaatacagc ggcatttatc
     2941 acagaaattg ctacttatgc tcaaccaatc gctcaagcca acgatctata tgcatcagtg
     3001 atgatcgcac aagcagttgt agaaagcggt tggggcagta gtgcgttatc tcaagcacct
     3061 tattataatc tttttgggat aaaaggcagc tatcaaggac aaaccgtcta catggatacg
     3121 cttgaatttt taaataatca atgggtaaat aaaaaggagc ctttccgaca atatccttct
     3181 tttgctgaat ctttcaatga caatgcctat gtacttagaa atacttcatt tggcaacggt
     3241 tattactatg ccggtacttg gaagagcaac acgaaatcct atacagatgc gacagcttgt
     3301 ttgacagggc gttatgcgac tgatcctggt tacgcaggca aattaaataa tatcatcgca
     3361 acttacggat tgacccaata tgatacacca gctagcggaa acgcaggcgg tggagcgact
     3421 acaggtggca ataatactgg aagtgcaggt agtactggcg gttcaagcaa ttctggtaat
     3481 acaaactcag gttcagcaac ttcgaccact tatactgtcc aatctggtga ttctgtttgg
     3541 ggaatctcaa ataaattcgg tatcacaatg gatcaattga tccaatggaa caacatccaa
     3601 aacaatttca tttatccagg acaaaaactg acaatcaaag gtagccagga aaatggctct
     3661 tcgacgaata attccggtaa caataccaac tcttcaggaa atgctggtac atcaaacgga
     3721 ggacaaacaa ctggtgcgaa atacactgtt caatctggtg attctgtttg gaagatctca
     3781 aatgatcacg gaattacgat ggctcaattg attgaatgga acaatatcaa gaacaatttc
     3841 gtttatccag gacaacagct aatcgtatca aatggcggtt cttctaatgc tggagcaaat
     3901 actggctcaa atactgggaa tacaagtggc tctagttcgt ccagctcaag ttcttctaat
     3961 acagcttcag gaacgaaata tacagttaaa gccggagact ctgtttggag cgtctcaaat
     4021 aaatacggaa ttacgatggc tcagctaatc caatggaaca atatccaaaa taactttatc
     4081 tacccaggac aacaactgac tgtttcaaac agtggatcaa caaacagcac gtcaggtaat
     4141 acaggttcgt caaccaatac aaataacagc agttcagtta atactggcag cagttcgtct
     4201 caaggcactt ataccgtgaa agcaggtgaa tctgtttgga gcgtggcaaa taaaaacggc
     4261 atcacgatgg atcagttaat cgaatggaat aatatcaaaa acaacttcat ttatccagga
     4321 cagcaattga tcgttaaagg tggaacttcg gcaaatacaa atacaggttc aactacttct
     4381 gcagcgaaac caaatacacc gaatacgtct gctacgacaa gtacatcatc cactggtaat
     4441 acgatgtata ccgtgaaagc gggcgagtca gtttggagcg tagcgaacaa acatcatatc
     4501 acgatggatc agttaatcga atggaataat atcaaaaata atttcatcta tccaggacag
     4561 caagtgattg tgaagaaagg ttcatctcaa tcaacaaacc aacaatcagt tactggttcg
     4621 aaaacctata ctgtgaaatc tggagaatct gtttggagtg ttgctgacag tcatggaatt
     4681 acaatggctc aactaatcga atggaacaat attaagaaca actttattta tccaggtcaa
     4741 acgttgattg tagctaaata aaatagctgt tcgaagaaat aacgattgaa ttccacaaaa
     4801 atatttgtta ctataaggct acttaaatat gacaacactt gaaaaggaag tagtaaaaag
     4861 actgaagttt cagagagcgc atggctggtg agaatgtgca cggatgcttt tgaactcgcc
     4921 tttgagttgc ccttgctgaa tacccgagta agcaagcggc ggtgacggcc gttacaacgt
     4981 tcgaggcctt ttcactaagg taaaattagg tggtaccgcg tagattacgt cctataaaaa
     5041 gagcaatctt tttataggat ttttttattt cttttgtgaa gtttgagaaa acaaagcaaa
     5101 aggaaataaa aataaacaga ttctgccggt tttttcgtta tatttataat agaaacaagt
     5161 gttttcaata gaacaggagg acagcaaatg agttacaatc ataaagaaat tgagaaaaaa
     5221 tggcaaaaat actgggcaaa aacgaatact ttcaacaccc atgatgatcc tgaaaagcca
     5281 aaattttacg cactggatat gtttccctat ccatccggtc aagggctaca tgtagggcat
     5341 cctgaaggtt atacggcaac ggatatcctt tcacgtgtga aaagaagtca aggatacaac
     5401 gtgctgcatc caatggggtg ggatgcattc ggtctgcctg ctgaacaata tgcgctagat
     5461 acaggaaacg atccagctga gtttacccaa aaaaatatcg aaacattccg acgccaaatc
     5521 aattctttag gatttagtta tgactggaat cgcgaagtga atacaactga tccggagtat
     5581 tataaatgga cacaatggat ctttacgaag ctttatgaaa aaggtctagc ttatgaagca
     5641 gaagtagcgg taaactgggt acctgaatta ggaacagtta tttcaaatga agaagtgatc
     5701 gatgggaaaa gtgaacgtgg tggctatgat gtagtcagaa aaccaatgcg ccaatggatg
     5761 ctgaaaatca ctgcttatgc tgatcgctta ttggatgatc tagaattagt tgattggcca
     5821 gaaagcatta aagaaatgca aagaaattgg atcggtcgtt cagtaggagc aaatattgaa
     5881 tttaaagtgg ctggtacgga taaatcctat accgtattta cgacacgacc agatactcta
     5941 ttcggtgcaa cttactcggt tcttgcacct gaactagatc ttgttagaga aattacgacc
     6001 cctgaacaaa aagctgctgt agaagcttat atcgaagaga cagctaaaaa atcagatcta
     6061 aagagaacgg atcttgcaaa agaaaaaaca ggagtattta ctggtgctta tgcaatcaat
     6121 ccagttaatg gcaaggaaat cccaatctgg atcgccgatt atgtattatc ttcatacggc
     6181 acaggagcca tcatggctgt tccagcacat gatgaacgtg actttgaatt cgctcaaact
     6241 ttcggactag aaattcttcc agttatcgaa ggtggagatg ttgaaaaagc tgcttacaca
     6301 gaagatggta cgcacatcaa ctctgaattt ctagacggca tgaacaaaca agaagcaatc
     6361 gataaaatga atgaatggct agaagaaaat ggcgtaggga aaaaagaagt cagctatcgc
     6421 ttacgtgact ggctattctc ccgtcaacgt tactggggtg aaccaatccc aatcatccat
     6481 tgggaggacg gaacagtcac agctgtccca gaagaagagc taccattacg gttgcctaag
     6541 acaaaaaaca tcaagccaag cggtactgga gaatctccat tagcaaatat cgaggagtgg
     6601 gtcaatgtcg ttgatcctgt aacaggtaaa aaaggccgtc gcgaaacgaa tacaatgcca
     6661 caatgggcag gaagctcatg gtattacctg cgttatatcg accctcataa caaaaaagaa
     6721 ctagcagatt atgaaaagtt gaagcgttgg ctaccagttg acatctacat tggtggagca
     6781 gaacatgcgg tacttcattt actatatgca cgattctggc ataaattctt atacgatatc
     6841 ggtgttgttc ctacaaaaga accattccaa aaactataca atcaaggaat gatcttaggc
     6901 gaaaacaacg aaaaaatgtc aaaatctcgt ggtaatgtag tcaatcctga tgatgtggtc
     6961 gaaacttacg gtgcagatac actgcgcatg tacgaaatgt tcatggggcc attagatgct
     7021 tcgattgctt ggagcgaaaa tggcttagaa ggaagccgta aattcttgga tcgtgtatgg
     7081 cgtttgatcg ttgatgagaa caataaaatg cgtgaccgta tcactacatt gaatgatggc
     7141 aagcttgata aagtgtatca tcaaacagtg aaaaaagtga cagaagacta tgaaaatctt
     7201 catttcaata cagcaatctc ccaattgatg gtctttatca atgaagccta taaagtggac
     7261 gctctgcctt atgaatatat cgaaggattt gttcaactac tcgcaccgat tgctcctcac
     7321 atgggtgaag aactatgggc aatccttgga aatgatggtg gtatttctta cgcaccatgg
     7381 ccaacatacg atgaagcagc acttgttgaa gacgaagtag aagttgtttt ccaagtaaac
     7441 ggtaaagttc gtgcgaaaag caatgttccc cgagacttag gaaaagatga gctagaaaaa
     7501 gcagcattag ccaatgaaat agtgcaagaa tatattgaag gcaaaacagt cagaaaagtt
     7561 attgcggtac caaacaagtt agtcaatatc gttgctaatt aattggctct ttgtcaataa
     7621 ggactgatga gttgtgcaaa atcaaatctg ggccagaatg aacttcattc tgtctcagat
     7681 tttttgttat ttcaaattga ttaattgatt gatcaaaaag gttttgattt tcatgttctc
     7741 aaacacgttc aatgtctttt tcttctgaat ggtcttgtaa gagatctctg taatcatcaa
     7801 atttttgtct attaatctta gtattttttg atgtataaac tacctgaaat tgagagtaca
     7861 taccaaattc ataaattttt tattagtggg actagtgatt gtatggattt tgacgcataa
     7921 tgaatgaagt atgagaaaag gagagacaaa tggatcaaga actatttatt atcgaagtat
     7981 tatcagctaa acaaaaaatt atcgagaaag cgaaaaaaag agcgaaaaga ataatgttca
     8041 agcttttaag cttagtattt ttctctggtt tgcttttttt cggagctgta tcgttcctat
     8101 atcgccatat gagtgcagta gtccgttcag ctgaagggat gcaaaaatta aataatttgt
     8161 atcacttttc gtggggagtt ggcggaataa caattgttgt gcttattgtt tttcttctac
     8221 ttatttggct ccgttaccat caattgtcgc gtcatttcga tcaatggctt agtcaaagag
     8281 cacaacagtt tttacataat aaaccaactt ctcaagatga tgactatctt tattttgaga
     8341 agacttcgac gagtcaagct tttcggatac aaaaaagtga ttgccagtta ttgctgagat
     8401 cacttgatga tttacctatc tacttaggcg aaagcaaatc aaaattcgga atgaacacat
     8461 tccatatttt tttactagaa aacgaaccca gatgggaaaa aagccagtct tcgcctggtg
     8521 gatacctgac aactaagatg agtacattag tcactggttt tttcctcatt cttttagctg
     8581 gaacaatcta ttatgggaga tacactcagc caaaaatgtc ttctcatata aatgctgctt
     8641 ctcacatgac aatgaactca actaacgata cgcatataca aaagacaaat gaagggttgt
     8701 tagctgatcc taagcaaaag gtaaatttga caacacaaaa gataaatgat ctcaaacttg
     8761 atacagagac gcactctctc tatatgacaa cagatagtgg tgctaattgg gtatttgtgc
     8821 cgattgattc gcaatggttg cgttttggcg aatacaccct gacaacgggg ctgattcctg
     8881 aaggttattg gatggacaac acatatgata tttccgccga tttctcatgg tttatctatt
     8941 cttctgggga tgatgtacgt ctgctgactt caagagataa tggtaaaaca tggcaaacgt
     9001 ctattatcac ccaatcagtt cagccgatac gttatcgaaa agcggtattt tatggcaacg
     9061 atggtctatt tatctgtaca tcagccagtg ggatgtctgc agaaactttg tacatttaca
     9121 ctactatgga tggcggaatc acttggataa aaagtggaag tacaatgatc aatcagccag
     9181 tacaaaatgt cagcttcgtt acaacttctt taggttttgt ttcgacacgt gaaagtttgt
     9241 tttatacaaa caacggagga cgctcattca aagaatcagt tctgtcgatt ccggaaaact
     9301 atcagacagg ggggatagat ctttttcaat caccaaatga agttactcaa ataagcgcga
     9361 atacgttaga gacaaaattt tatttgttga aaaaaggacc aatcgatgca gggaaaatgt
     9421 ttgcctgcct cttccgttca actgataacg gggaaacatg gcaattcgtt cagcaactat
     9481 cgcaagtaga acaaggagat taaagaagag agattgtgag cctgacgttc agcaccgagt
     9541 cacaagaaac aaattcaaaa aacagcttct catatttgtt gaatagtgta actcgtgatt
     9601 cggagttgac aagagcaaca agaagcgatt ttgaagtata gcttctcgta ttttacaaga
     9661 tcgtgtctta ttgccgatag gttgtctctt gaacaccgtt tattcgtaaa aagggattgt
     9721 gacaagtctt ttgtcactac ttaccgaacg aaactgaagg agctatccaa aaagtatcat
     9781 tagtattatt ctttgtgttt ctagtggcac cactagtaca tttaaaaaag ctatcgatca
     9841 tggactaatc atacatgatc gatagctttt tagataatgt ttgttacgct atctgaaatt
     9901 gactattata aagactggca taaagcgttg gtgattggag tagttcagaa tgtgttcctt
     9961 tttcgacgat atcgccattc ttcattacaa ggatcatgtc tgcattttca atcgttgaca
    10021 aacggtgtgc aatcacaaaa cttgtcctat cttttgtcac agcttccatt gcttgctgaa
    10081 tcaatgcttc tgttcgtgta tctacgctag aagttgcttc atctaaaata acgacaggag
    10141 gattcgctag aatgactcga gcaattgtca ataattgttg ctgtccttga gataacgtgc
    10201 cgttttcgct agaaatgatc gtatcatatc cgtctggcag tgttcgaata aagtgatcac
    10261 attgtgcgat ttcagccgct tcgataattt gttctcttgt ggcatccatt ttcccgtagg
    10321 cgatattatc agctaccgtt ccttcaaaca gccaggtatt ttgtaaaacc atcccaaaag
    10381 aacgacgtag ttctccacgt gaaagcttgg taatatcaag accgtcgaat tcgatctttc
    10441 ctttattcag ttcataaaaa cgcatcagca gattgactaa agtcgttttt cctgcaccag
    10501 ttggaccaac gatcgctacc gtttgttttg gctttactga aaaatcgact tgattcatca
    10561 atattttatc tgatgtgtaa ccaaactgta catttttgaa agctattttt cctttcggtt
    10621 gttctaaaag tcttgcatct ggaaggtctg gaatttcatc tgtttcatct aatatctcaa
    10681 atatcctatc gattgctgcc attgcggatt ggatcgagtt gatcacataa gaagcggtag
    10741 aaattggttc agaaatctga ttgatgtact gaagataagc ttgtaagaag ccgatcgtca
    10801 caccacctga tagaacaagc atagcaccga ataccgcact tatgatgaag gcgagttgat
    10861 taatgaaacg aatggccgga tagatagcac agttcataaa ttgagcacgt ttaaaagcgt
    10921 tgtattgctt tttatttact tcgtgtatag cagcttctgc atgagcttgc tgattgaatg
    10981 ttttgaccac tagattacca gctaagattt cttccattcg attatttaaa acacccagtt
    11041 catactgatt ctgttcagaa atctgtttgt ttttgttcgc aatcttagtt gtaacaattg
    11101 aactgccaac gatcagcaat aagacgagca aagtaagctt gctttcgata taaaacagca
    11161 tcagaccagc agcagctatt gttacgacag aagtaaaaaa ttgattgata cctgtcaata
    11221 acacttgaga aagttgattg agaccggtag ttgtccgact aatgatgtca cctacttgat
    11281 gattgtcaaa aaacgacatc ggtaatgttt tgaatttttt tgtgatttct tttcgcaccc
    11341 tcaatgtgat cttttcgctt aacgctgcca ttgtccgttc ctgaataaat gaagtgatgc
    11401 tgcttaagac tgaacagccg atcagcaata agacagggaa caacaatgct tgtttgatat
    11461 cactgaaaga aagctgtcca atacctgttt ttcttatttc tgataagaga tcatcgatcc
    11521 cgattcccat gatcaagggc atggctacga ctaataggtt cccgaataaa ctgcatagaa
    11581 acaatccgta aaaatattta tgttcaggtt tgatcatttt ccaaaaacgt atcaaagcac
    11641 gcgtgttcaa cgtttttttc ttttccatcg tatcccttct ttctatattc cttgtgattt
    11701 tgcaaactcc tgataagttt ggcaattaga cagtagttct tcatgagtac cttgtccaac
    11761 gatttttcct tcatctaaaa caatgatgtt atctgcttgt tggattgtac tgaggcgttg
    11821 agcaacaatc acgagagttt tctccgacat gtgtttatga agggctgctc ttaatgctgc
    11881 atctgtttta tagtctaaag cggagaaact atcatcgaat acatagatct ctgctggttt
    11941 gatcaacgct cgagcaatac atatccgctg tttctggccc cctgaataat tagaaccgcc
    12001 ttgagcaaca aagctgtcca atccgtcagg aagggtgtcc actagttctt ctaattgagc
    12061 gatcttgatc gctttagaca actcttctgt tgaggcatat tcatttccca tctgcaagtt
    12121 gcttaaaatt gtcccgctga ataagaaagc cttctgaggg acataactga tgtgggagcg
    12181 gatctgttct tgagtcattt gagtaagagg aataccacca agggttactt ttcctatcgt
    12241 agggtcacta agtctcaaga gaagtttcgc caaactactt tttccagatc cggtcccgcc
    12301 aacgatcgct gttgtttttc ctttaggtat tgtgaaatca atatcttcta gaactggatc
    12361 agagatttca gtatatttaa atgtgacatg ttcaaaagca gctgcatttg tctgatcaaa
    12421 ggttggttca gctactgttt tgatcgtctc ttcctcaatt tcgatctctg cttgcagtat
    12481 ttcttctatc cgtcgtaatg aggcaaacga tttagggata atgaccaata ccatagctgc
    12541 gatcatcaag taagcaagtg tcagcataga gtattcgact acagcagtca cgacaccgat
    12601 ttgtaatgct ccttctgcta caagatttcc gccaaaccac aaaatagaag cataaacgat
    12661 tcccatgagc aaaaaagcaa ttggagtgac ccaagcaaat attcggttca ctttgatcat
    12721 ttgattagca tagtcagcaa agctcttatt cgttcgcttt tcttcatatg attgattatc
    12781 aaaggcacgg atcatattaa tcccagtaaa gaattctctc aatgtgatgg tgatccgatc
    12841 catttttggc tggatactca aagataaagg tgcccctcgt ttcatcagtc gataaacaat
    12901 ccatacataa aaaacaacag agatcaatgt aacccatgtt agtaatagag aataacgaaa
    12961 catcatgaga atggtgaaaa cgcaaataac cggtgctggt aagatcattt ggcaaaataa
    13021 aacaatcatt tgctggacat tatcgacatc attcgtcatt cttgtcaaaa gagatccaga
    13081 actaaaacga ctgatatcct tcattgacag ctgttggaat ttacggaaat atttctcccg
    13141 cgtttggaat ccaaacctag cggaagccaa tgctgaaaag taactagagg caactgcagc
    13201 gattgttcct agaaacgcta caagcagcat ttcaactcca atttttttta taactgaggc
    13261 ttcgccactt gctatacctg tgtcgatcag ctttgctact aattttggta cacctaatgt
    13321 accggcgatt tctaaacaga tcatcacaaa ggtaaggaga gcgagtcctt tgtttttctg
    13381 taagaatgtt tttaagatag acatattatt cctccttttt tcctgatact tttcgattct
    13441 ataccttcaa gtaacttgaa ggtcaataga aaaaagaata aactgaaaaa gaggtgagaa
    13501 aaaatgaaaa aaatgtatac gattggccaa gtatcggaac tatttgattt gccaaaatca
    13561 acgatccgct attgggatga acaaggatta atccgttcaa gtagacaaga agagaatgat
    13621 tatcgattgt ttgatatcga tgatattttt atgctttatg atatcgtttt ttatagaaaa
    13681 ttagatatac cgatcaaaca gatgaagaat ttatatggaa aaacattgac ggagttatat
    13741 gagacacttg atgaaacaga aagaagaatc cataaagaac tggtggtgat gaaacaaaaa
    13801 caaaaagaga taagggagca aaaaaaacag ttgaaattga tgatcgatac aaacgaggaa
    13861 gaatttcctg tagaagagat cccttttgac tgtatgatct caacggagtt tgaagatatt
    13921 gtggaaatca aaaaatttct acctaactat tcaagctttg ggatgatgag tatgccagct
    13981 tccagttcac agacgatgta tggatttttt atcgacccta gtgaagtaca tttatttact
    14041 caagacgtga tttgggagaa aaaagatacg gctgtttatc gtcgattctt attgaaatcc
    14101 gagatgaacc atgcggaacg taataatatt ttagaaatca gagaaagaat gtatgaaaaa
    14161 ggatggaaaa caggagaagt gatcggccag tatctattga cgaatacgga tgaaaacaat
    14221 atcagaactg aatattatca tgcatggata gaaatgaaaa aataggaaat atgtgtttaa
    14281 gataactcga aaatgatggt tgttttagtt ataaagaaaa attttctggt tcgattacgg
    14341 gtttaatgtg ggggattatt agtcgtggat agatagaatt ttagtttttt agaggaaata
    14401 tgtctttaaa aatggaatgg ttgcaattct tagcgatata tacgatgtaa ttgtcctctt
    14461 aaaaaagtat ggaaaaataa ttgtattggg tgttatgtat aattcatggt gaactgagac
    14521 taatgagaag aaaaattcat attg
//
LOCUS       DACDEG010000062        14490 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.064, whole genome shotgun sequence.
ACCESSION   DACDEG010000062 DACDEG010000000
VERSION     DACDEG010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.064"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(316..738)
                     /locus_tag="IV142_001886"
     CDS             complement(316..738)
                     /locus_tag="IV142_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP8485541.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLNQFLPNF"
     gene            complement(728..2902)
                     /locus_tag="IV142_001887"
     CDS             complement(728..2902)
                     /locus_tag="IV142_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HAP8485542.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2918..5260)
                     /locus_tag="IV142_001888"
     CDS             complement(2918..5260)
                     /locus_tag="IV142_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485543.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(5282..7825)
                     /locus_tag="IV142_001889"
     CDS             complement(5282..7825)
                     /locus_tag="IV142_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HAP8485544.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7835..8233)
                     /locus_tag="IV142_001890"
     CDS             complement(7835..8233)
                     /locus_tag="IV142_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8485545.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(8314..8598)
                     /locus_tag="IV142_001891"
     CDS             complement(8314..8598)
                     /locus_tag="IV142_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485546.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8622..9620)
                     /locus_tag="IV142_001892"
     CDS             complement(8622..9620)
                     /locus_tag="IV142_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8485547.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
     gene            complement(9613..9960)
                     /locus_tag="IV142_001893"
     CDS             complement(9613..9960)
                     /locus_tag="IV142_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485548.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFLEGASVVVHEVVIVDKI
                     PKLLLERYGISHE"
     gene            complement(9976..10626)
                     /locus_tag="IV142_001894"
     CDS             complement(9976..10626)
                     /locus_tag="IV142_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485549.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(10637..11809)
                     /locus_tag="IV142_001895"
     CDS             complement(10637..11809)
                     /locus_tag="IV142_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP8485550.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(11828..12700)
                     /locus_tag="IV142_001896"
     CDS             complement(11828..12700)
                     /locus_tag="IV142_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8485551.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(12722..13642)
                     /locus_tag="IV142_001897"
     CDS             complement(12722..13642)
                     /locus_tag="IV142_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485552.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(13642..13911)
                     /locus_tag="IV142_001898"
     CDS             complement(13642..13911)
                     /locus_tag="IV142_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485553.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(13908..14288)
                     /locus_tag="IV142_001899"
     CDS             complement(13908..14288)
                     /locus_tag="IV142_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAP8485554.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
BASE COUNT         4046 a         3109 c         2285 g         5050 t
ORIGIN      
        1 tccccccact tattacatat aacctttaaa taatctctaa ataaccctca aataatcctt
       61 actaatactg aaacgtaaaa aaaataaacg gatttcaaac aaaatacgtt tattttttct
      121 attgatttta gctcaaaatc gattcaattc gagtgaaatt tgcttcaatt ttcaattggt
      181 ttttgttcaa attttgaaac atttttgctc aaaaaatgag tgaatttgtt tcaaaagaaa
      241 aacaaatacg atcgaaaaaa atttgaactc ataacaattt tttgtttttt tcaacgtatt
      301 ctctttcaat tttcactaaa aattaggaag aaattgattc aatgtctttt caaaaaagaa
      361 tgtttttctc tcaatttttt tgacgtttct atcatgaatc acgagaaaac ttgggacact
      421 tttaacatgg taacgttgta aaaaagtttg aagttcttct tgtcttggtt cttttcttgt
      481 ttcaattcga tatacattcc tagagaattt tttgtcgtat ctttccaaga aagattcaac
      541 ttcttggcat tttttacaat catttcttgc tagatacaca aaaaatgatt gattcgtttt
      601 ttgaagttct agtacttctt ctaaattact attgattagt tttccatttg attcttcgtt
      661 agtttttatc agtttaaaac aaccaaataa gaggaaacta acaataagta tcctaacaat
      721 aactttctta tttcgcatat tgtttctcca aatttggttt ccgaatatag acgactcgcg
      781 gtggttcctg gacgtcttca gctgttcgat acaccacaaa ttcgcctgtt ctcaatgctc
      841 gaatgcgatt cggattgaat ttaaagcggt ctacgtctcg tttcgtgcct ctttctcctg
      901 tccaatcaac ctttcccata tcagaataac ctgcttcttg tgtcgtcatt tccgttaatt
      961 caatgtcttc ttctgtcccc attaaatctg cccaataatc aatttctgtc gggtcatttg
     1021 tcttcccaat aaagaacgta ttcacattcc caatcaactg ttttcgaagg acaggatcaa
     1081 ttctatcaat gtccgcaagc gtttgagggg catacgccgc atgcacccca gcgcctcgtc
     1141 ccttattgac taagtctata aggttatcat ttagataaga agctggttcg tcaaatatta
     1201 ctaggacttt cgagcgattg gctgtttctc ctgcttgttt atacacttct gacgcaaaga
     1261 aattaatatc tcccacaagc atttgagcca atgtccgaat aaactcacta taaatcaatc
     1321 cgtttaacga gatataaaca atctttcctt ctttaacaac ttcttgaata tctaattctc
     1381 tttttcctcc agaagtatca aagagttcac caagctcact atccaataac atattgatat
     1441 tggtctgtaa attctcagaa gtcgcataca aataaaacgg gttcttctta tgttcgtaac
     1501 gaacaaacaa ccgtttaaac aacgtttgct cgatttctgt catgagagaa atgttttctt
     1561 ttaataatag ataatacgtt tctaagtcaa cggttttacg atcatgttca acgattcgct
     1621 cttcacttaa ctgttccaca tcggcaaaat cacgttctgt tttgtccatc gcttcattct
     1681 ttttcgtttt tttcttcgtg gttttcttct tttctacgac cactttttct ttaaataaat
     1741 ctggattttt caactgaata cgatccgcaa acaaatgtaa gacgtttcgt ggcaataaat
     1801 aatgttgtaa ccaaactaga tttctttcaa ttttatggct cttttggaac tcgtctaaaa
     1861 gctgaatcgt tacctgtaat aacgctttgg ctgctgtcga ataatacacg ctttctgttt
     1921 ctgctagatt ggttaattta tctcgaatgg caatatcatt gccatttttt acaggattat
     1981 aacgcaaatc gccaccgtcc acaaagactt caatcgggcg attgtatgcc tctgccactt
     2041 cttttaacgc ttgtaaagtc tctcctgctc ctttcccatc gactaagata acggggatac
     2101 gaaacttaca cgcatgttct gctagtaatt ggatcaccgt cgtttttcct gaacctgtag
     2161 tcgctggaat aatcacatgt tggttcaatt cactaaagtt catcacataa gggagcccat
     2221 actcatctgt tcctaataga atcactttgg ctaatgaagc cagttgttta ttccgcaaag
     2281 caacgtcttt tatgacaatt gctacctggt acgcttgctg atactctgct gtatattttt
     2341 gggactcctg aaatttattc ttccgacgtt ccttgtattc tttactattt agcaaagctg
     2401 tttgccgttc tttctttgaa acaacttttc ttttttcaat aaaagacgca tacgcaatca
     2461 aacaacttcc tgcacctgtc ccacctaaaa ggagggcaaa aatcgtttgt ccattaaact
     2521 gcaacgcatg agaaaacaag tttaatggaa ataataagta acacgacaaa taccctctag
     2581 gaagaacaaa aataaaacaa aatagattta atagcgtaaa acctaacgca aaaataaaaa
     2641 aaagagagct tcttcgtttt aagaagctct catactcaaa gtcataaata tgtgcacgtg
     2701 tctctttttt atctatcata aagacaatcg ctaaaggaat actgcctaca ataaaaaatg
     2761 gatatagaca aacacctaat aaagtgacga taaaataaag ttgttgaaac cgactttttg
     2821 gcgtttcttt ttcttgtttt cttttgcgat cgacagaaaa catttgctca aaatgtgtcg
     2881 gtcgttttgg tgcattcatc atactatcga tttcctccta tcgttttcgt tgtttcattt
     2941 ctcttcgata aatcggttgt ttttctcgtt ttcgtttcat ttttgcttga gttgctcgaa
     3001 actcactccg tgtcttcggt gcttgttttg gtgattttgt ttgtttcatg gttgagtatg
     3061 ctttagatac ggttgaaggc gctttggttt gtgctgttac tcgattgact gctgacatat
     3121 gttcccgaac cccctctgga cgtcgaacag atttcggtgt ggtagaacct gttgctcgtg
     3181 gtacattccc ttttcttagt ggtttttgta ccgcatgttt ttgtcctgca actgtccggg
     3241 ttttcatcga acttgttttt gttacgggag taagtgcctc cttccgacga cgacttgttt
     3301 gtccctgacg acgttttcga tcgttcaatt ctgttctagg cgtatgcttc tggccataag
     3361 acttgtgttt ttgctttgga ttcgctaatg acaccaccat tgcagaaccc tgattgcgat
     3421 acggactacg agacgcacct tttctacttc tttctcttag actaggattg ccttgcaatt
     3481 ctcgttgtaa taaatcggct gtttttcttg cctgtttctc cattgctgtt cgttgcatac
     3541 gttcttgact ttgctcttct gcttgtttcg cttgatcatg caagtctctt gctttttgtg
     3601 ccatacgttc catacgattt ggtgctttat gacgaggttg ttgtgcggat tcacgccgaa
     3661 aagcacttcg tgtggcttgt ttcgccgttt gtggcatacg aacgtctgag ccacccccac
     3721 gttttcgttg acgttcttgt ctcttccgtg cgttttcttt ttctttttgt ttctgtgctt
     3781 ttctcgcttc tttgcgttct ttcaaccgtt gtttggcttc ccttctcaat tttgcttctg
     3841 ccccaaatgg atttcccgca aaagctaacg ctcctcgaac acccattctt ccagtaaata
     3901 gactgcctaa aaagcctcgg aacaaataaa ttcccaacgg cgcaaacaaa taaacaatga
     3961 tcaagagaat tttttcccat ggatcgtctt gtgtactact taattggaac ccgtaactaa
     4021 gaaaagaaga aaaaaggata catgcaaaac ctgtcattcc ttttaaaaac gtcaaaatac
     4081 caaaaacttt ggcatagtta ccaaacacat tcattgtgac aagaaacaat cctaaaaaaa
     4141 gtaagaacgg caacatcggt actaacaata gttgcatagt tccaataatc aatcgaaaca
     4201 tacttaagac aaaataaaca acggtttgaa tcagattcac gacaaaataa aacaaggcaa
     4261 atgctgtatt tttccaacta ttgtaatatt gtatgttctt gtttttcagt gtatctgctt
     4321 catactctgt gactttgtca tgaattttct cccctatttt tgtacctaaa tcattgtcca
     4381 ataaaagatt gattcgatca tattcttttc cagcatattt gactttcttc tcacgaattg
     4441 tgtctacatt ggttgtgcca taatttaaca acagatatgg ttcataaaca ttactataaa
     4501 agacccgagc cgccatcaat ttgcctgccg tttccacttt tttatccgct gttacctctt
     4561 ctgtttcttt tgtttcataa tttgtttctt cttcaggtgt taacactgga ttgaccttca
     4621 cgaacgccgt ttccactgta gaatctaaag aaaaaagaac atcaaaaaga ttcttatttg
     4681 tcccagaatc ttttaatacg cttaacccag taaaaataag gatcgtcata agaaagacac
     4741 ggagtgcttg tttcattcga gcctctccag catatttgat tgccatcata attgccgaaa
     4801 aactgattcc aatagttcct gcaatactta acatattgtc cgcaattttt tgtgttaagt
     4861 tttgaatggg ttctcgaatg gcggaaataa tatctaattc aaagagaaat tcaaccattg
     4921 aagcattgaa ttctcctaac atttttacgc cactccaaac aaaatttttc atcactcctg
     4981 caacattgta cgatagcttt tttttaaggt tcattgactc atagtcattg gtcatcaatt
     5041 caaacgaatt ctttttgtaa tttttataca attgatccac atcagtcgtt acttctttta
     5101 cttctacttt atcgttggca ttctctcctt cagaacgatc ggtttcagtg acacccgcac
     5161 catcattcat ttgatcggca gaaacagtag ccccttgtag acagaaaaaa agacccccta
     5221 ataaaaggag cgtccaccat ttcagagatt tttttcgcaa aaaaaccacc tcttttttgt
     5281 tttatcgttg tttttcattt tctaacattg cttgaatttt ttcatcttga ttttcagtag
     5341 aagaaacagc caataaccaa ctatcaaaca acacatcaat ggcaatcggt tgatttcttc
     5401 ccaaatgatc ctggaataaa caggttcctg tttgtaattg ttccatcatt tctaaattgg
     5461 ctgaattcac ttccatatca aagaaggaca atagtttctc tcgtgcttct ttttgtttcg
     5521 gacgaaacgc aaatttgtaa gaaattaact cttttttatc ttcttgatcg taatctttaa
     5581 acgcttgcac aacaaggtaa atatccgtat tattcgcacg tccttttcgc aagctatcat
     5641 caatcaaata gcttccttgt gcggtatctt catacccttt tgcctcgtca taaataatcg
     5701 cagcgtcttc ttctttgttt gtagaaaaga tatacgtaaa cttcataatg acttccataa
     5761 ttgccattcc tgcaagttgt tcgctattta atcgacccga atcaatctct ttttgtgtcg
     5821 gaatgcgtat tccttgtgtc cctaacacgt tgatttgtga atcaaaacgt atcggcgtac
     5881 tgtgctcatt cccaatgaga atctttccta aaccaacgct atgccctaaa atcaatttag
     5941 ctagttcttt gtcacttttt cgaatttctt cgataacttg acttaacgaa tgcttacctg
     6001 gcatggtgta aactacatcg atggcgtctt ggatcaccgt tttcttgtgg ctcgctgttt
     6061 ctcgattggt atctacttct cctaacattc ctaacacatt tttagcggtt tgaattgcct
     6121 cttcttttgg taaaaacaat agaggatcaa gcatccctcg acagctttca tctgaagata
     6181 acgaaataaa attaattcgt tcatacatag ccacaaactc tggtatgtgt ccgaattttt
     6241 caagtgcctt taaaaagaac ttttgggttt catttttcgg atcaatataa agaatttttt
     6301 gacctaaaaa catcagccat aaaaaaatat attttacaag gaccgatttt cctttacctg
     6361 gtggaccaat aatcaacgta tttccattgg tatgttccgc gccttcaata cttttctttg
     6421 ttaaattggg ataataccat actagtttac tagatagcgc taacgcttct ttcgcagatt
     6481 taaacttttt tgcaataaca atccgtccta aaggaaatcc tttcttattt cctacttctt
     6541 tctttaagtc caagccataa tctgccacaa aacctgtcgt agcgacctgc tcgtaactac
     6601 gaaaacgttg cgtacttcca attaagcatt gattaaacaa cgttagctga tccgctaccg
     6661 atcgataaaa tttgaaatct gtagactcaa tcgcaaattc aatatctttg actcgttctt
     6721 ccaattcttc ttttgaagaa gcagatacaa ccagcgtgac ggtagaataa cacaatcgtt
     6781 tttctttgcg cttcagctta tcttttacat ccattaaccg ttctgtacca aaatttaata
     6841 catcgtctga atctaaaatg ccgtcaacgg tctcatattc tttttcttgt tcataaatcc
     6901 gtccagccat tttattaatt tttcgttcat cagattttga atgctcaaat cgaagtcgaa
     6961 tatgcgtatc aattggaaaa gataagctat cctgaagatt ttgaataaag ctactcccat
     7021 acatggctaa aggagcgtcc gtttgactta aaaacgtcga ataatgcgtt tgattcaact
     7081 gttcaatcgc aatgtatcct gcttcattcg aaatgacgcc ctcgcaaatt tccattggcg
     7141 taagctgtct ttcaggcaat ttttcttctg atcgatggaa aaaataataa taaatttgtc
     7201 caatctcttg ggcagataag cgctctatcg gcttataatt ggacaattcc gtatagagct
     7261 ccttttctaa tgtttcatac gttggtaaca cactacttat aggaactcgt tgtcccgtaa
     7321 tacgatagat aaacttttta aaaacatgct taagcaattg aaaatattcg atcggatcag
     7381 acactttcat ttctgtagtg aaacgcacaa acagatacgt tcgatactcg tttaattgca
     7441 cttcgtcttt taaaatcttt cctgcctgtt caaagtacac tttcccaaga tctgcgcatt
     7501 ctccctttac caaatctcga atggtttctt caatgtgcgt atctaggtta aattgacgtg
     7561 gaatattgat aaaatgatag tcatattcat catgtccaag tatgccttcg ccatcttgaa
     7621 gatagttctt gaaaaaatca atgtcattta aaggaaatac ttgggaagaa agttgatagc
     7681 ctacccacac atcatcattt ttggtcaaaa caaaatttcg gataatggcg tctatcggaa
     7741 attctaatcg aggaagcttc gtttttttct ttttgaactg ccaaatcccg ataataaaga
     7801 cgaccaaaag aaacaaaaac gccatttact cgactcactc cttccatttg tgtagggaat
     7861 acgtcacttt ttcatggaaa aacggcacat acatttcatg ttcaaagcgt tgtttccgtt
     7921 tctgataata ggacgttcgc ccaattaaat actcgaaaaa gtgtttattg tcccatttca
     7981 ttgaaaacaa tgtccaaacg agaacaccaa tcccaaaaat cataatcaac caggactttc
     8041 gaaacagtgt ctgcaagaaa ctaatttgat tcacatacgc aatcaaaaat aggatcaaaa
     8101 gaatgaagag aaatacccca cctatgacta attgttccat gcgaatgcca ttagaaatcc
     8161 agggaatttt ttgtccttta atcccgtaaa ttttgtgagg gtgcttaaac tcctttgtat
     8221 agttgtattc catgttcact ctacctcctt ttacaaaaaa agaagcgacc agaagttcta
     8281 atcttctgat cgcttttgca gtttgtcttt ttcttatgat aattgtatcc agggcacaat
     8341 catttgcgtt acccccacgt tctcttcact atccttaata aagataatgt tctttagcca
     8401 ttcaccaata tcaacggcat tcgataacaa taagacgagc attatcaaaa taaagacgcc
     8461 aataatggca aacgctgccc gcgcaaagcc ttgggtcgca taagcaccaa ccattagaac
     8521 aatcgcaacg attgccaata gccaaatgac ttgtccagat acatcatcaa ataacgattt
     8581 aagatcaaaa agtttcattt ccattctcct ctaagttcat tttattcatt taacgtagta
     8641 acaaaataac tatcgccact tgtttttact tccattgaaa agtttgattg gaactcgttt
     8701 cctttctcaa aatagaaacg atacttccct tgcaaaaagt agtttccttc tttatttttt
     8761 actgcatgat ccagttgtac attgatcaat tcgccgcctt ctaacccttt ttcgttagaa
     8821 atcaatgcca atttttcatc atttttgaca tataactcaa aaaaccgttt cacaaatgct
     8881 tccagcttga tttcttcgct ttcagtgacc gcttgcccag aagaataaaa tcgattttcc
     8941 tgatagttca cagaattggt attcttactc tcagaaagat tcaaaaattg aacgccatca
     9001 agtactttta aaacattgtt ttgataactg actggaaaag aaagttgact gcttttccaa
     9061 tgttctcctt ctgtaaaagt aacctcataa aataggcgat acattgcttg tgtcaaatcg
     9121 atttcttcta gtttaataaa gcgaacatct ttcgctactc gattggtagc cgtcgttaca
     9181 aagcctagat cagacgcttc caatccttgt gctaaataat gattgacgga agcctcccat
     9241 tcgtttcgtt tttctggcga ataagtaaat aacctttcaa taaaatgttg tccctcgtac
     9301 ttcaattgtt ccgtagaaga caaggtttgg ttgacttcag ttaacaatgt ttctggattg
     9361 acttgttttt tcactgctaa acgcgttaat gtatccactc gtccaaagtt taggaggagc
     9421 acgacaccca ttaacagaaa caaacaaaaa aaagcgcctg aaaatacact catggcactt
     9481 ttttgcgaaa attggatcat tcgatctgac ggttctgttt tctttttttg gaacaaaaaa
     9541 cgtttctttt gtttcttttc cttctgttgt ttcggtggct tagtttggaa cgcttgtgga
     9601 ttaaattttg cattactcat gagaaatgcc ataccgttct aacaataatt tcggtatttt
     9661 gtccacaatc actacttcgt ggacaaccac acttgccccc tccaaaaagg caagtgcttc
     9721 tttgtaggca ttttgactgt atttttttga taaagcatga caaatttctt gataaaatag
     9781 ttgactgcct accgaatacc gattattgtg tatccgccac aaaacgaaca cactgatttg
     9841 aatcgacggg cgtaaaaaag aaaaattctt tccataagaa aaagaacgtc cttcttgggt
     9901 ttcttttgct tgttctgttg gtacttcttg tacctctctt attgattctt gtctctccat
     9961 aaccttctcc ttttcttatt cttctaaaag ctgttgatta aagctacttc ccaaattttg
    10021 cataaacgtg attttctttg acgctggatc aaaggttacg tcaaaaatat tcatggcatt
    10081 attccaggaa ctatcaccaa ttttattgcg atacgacacc acaacgagcc cttggatagt
    10141 cgctttttgt gtgcttttta aataaacaga acatttctct aattcagaag acaccccgtt
    10201 tgaatcacca taggaaacgc cttctttgaa atattgcttc atcagtttgt cagaaagata
    10261 tggtgcacaa ttgttttttc gttcttgaaa agaatcaggc tcataattaa acagaacctc
    10321 gaacgcttct gtcactgttt tttgaaaggt ttgaatgttt cctttttctg ttgaatcatt
    10381 gtttttcaat tcttcatttg ctttttttaa cgtcgtgacc tcgctttcta cttgttgaag
    10441 ttgttctttg agtgacgtta caaggtcttc ctgtcctttc ttctcttctt ggaacttgtt
    10501 tatctccacc gtcagtttct cttcctttgc tgttagctgt agcttttcat gaataagaaa
    10561 aaatgtactg cctacaccaa ataagaacaa tccgcctaca caaacataaa taaatcgttt
    10621 cttcatttga cacctcttat ttctttatcg gtcgagcaaa acaaatatca ccaccattaa
    10681 gcaaataccc aaaggataaa ttgacttgcc cttcacctac accgttagta ttcattcccc
    10741 cttgttcgat tactttcgta gaaagtccat gccaatcttc cgctaaaaca gctgtgtgtc
    10801 catcatttcc cacaccagaa cctaagttca caatgataat atcgccagct tttgcctcgc
    10861 tctcaggtat ttcttgtaag tattgcttac ttcctcttgc gtcgctcgtc attgagccgg
    10921 taaaccaccc aacgttcgca ggcgttttat accctgcttt cgttaaaaca agccacacaa
    10981 agctcgaaca atcggcatag ccatttttat ctggattttc gacactacca aattgtgccc
    11041 gttgcggttg tgaataatga aaataaccaa tcagactttt tgctacgttt aatacattac
    11101 tatcggaaga actgcttgtt cctgttgttt ctagacaata atcggtcaag tctttacttc
    11161 ctgcgtttgc cccattttct aatgtaattg aagcgacggg atcactgtca ttctttccta
    11221 gttttgcgta ccattgattc gcaaaagtaa tacgattccc catcattggt atccctgcac
    11281 gctcaaattt cacttgccac tgttctgttg cttcctctac gctttttgct tgtaatgcaa
    11341 cttgtgaagc tttttctgtt gtgtttagtt catgaatcaa aaactcaact tgtacaccta
    11401 aatctgacca ctctttgttt cgctctttgg caaaattttt caaagcagtc gttcgccctg
    11461 ccgtccattg tgcaatccca tgcccagata atggatcgtt tggtcgttca atcgctgtgg
    11521 gactcatact cgactcttgt tgaaaattgc ctaacatacc cgctagaccc tctaatgtcg
    11581 tatttggaat atgtttttgt acttcctttg caatttgaag cccgtttgtc tccatgcctt
    11641 ggttcgttaa tgagatagta cttgcgacaa tttcggttgg actacagtcg atttcttcct
    11701 cttcagaacc actactaatc aaaagtatca taaacagaaa accaaacata catagtaata
    11761 gtgtgggtag acaaccgaac ccacaccata aaagaagttt tttcttcatg tttgatcctt
    11821 cattcgctta gttcattttt tgtgttgctt ttcttttgcg ccaaaaagca aaaccgcttc
    11881 ctgaagccaa taatccacca agtgctgtcg caacgagaga cacttcttcg ccagtcttag
    11941 gcaatacttt catttttcca tcagaccctt tcaccgcaat atttccgctt ggaagttgtt
    12001 cgaccttgtc tgaaatgggc gtcaagccat ttcctgattc tttataagcg acaccatcaa
    12061 cgacggagac aattttttct cctgaagctg tttcaattgg tgtacttggt gttggaatat
    12121 gggttaattc agacgcatta ttggtttcaa tttgaacatt ttgtccacct gtttgttggc
    12181 tgccaatact tccatctggt tttactacga cgtcctgtac tggaggcgta gtgttcgtcc
    12241 ccccattatt ggtaggtggt gtcgttggag gagtcgtatt tccttcattg cctcctggat
    12301 tcgacggctt ttctggtgtc tctgtattac caccatttcc tgggttttcc tcaacgggtg
    12361 gaacttcagg tgttccttcc cctccgccag tatctggatt ttctggtact tcaggcgttt
    12421 ctgtgcctcc acctgtatct ggattttctt ccgttggtgg aatctctggg gtttcaggag
    12481 tttctggtat ttcgggaatt tctggggttt cttctcctcc atctgttggt ggtaaaacag
    12541 gttcttcagg aacttcaggt gtttcttccg ttggtggaat aatttctgtc ggtggtacac
    12601 ttggatcacc tggtgacgta gtatctgcat acgcactggt tcctaccata cctagcaata
    12661 cagttgcact aaataaactt cctaaaatca tttttttcat gtgtttttcc tcttctctta
    12721 tttattgatt tatttcaaaa tcatctagtt cgggaagcgt ggaaactaga taattttttt
    12781 cctgttcttc caattgaaaa gacacttttt ttgtttcttg tgtctttttc tcggtttcca
    12841 atgtaatgac atacgcaatt tgccaatgct tgccttcttt cttcatttcc caggataaaa
    12901 tacttttgac tttttccttt tgaggactga tttgtttcgc aactttttcc gttagaaacg
    12961 attgaagctt tttcctatac cgttcttctg ccatttcacc cgaataataa ttcgtaataa
    13021 aataacgact aaatgtatca atttgtggtt gttttgtcgc aagttcttcc gcaaatgcaa
    13081 tgcgttcttg taactggtgc tcctttttcg ttagttgttc aatctctctt tgcgttgaaa
    13141 gttgcataat aaaaagacca caagatacaa cgacgataaa tccaaacaaa agccattgaa
    13201 aagacatttt tttcttctga agagagccgt tgctttgtgt aaacgctgtt tctacagaaa
    13261 gtggtgtttt cttttccttt ttgatttttg ttggtcgacg ttcgttcttt tggtctttct
    13321 ttacttgata aaacttcggt aattcggaat ttaaggtttg aataaaggtt tgtgcttctt
    13381 cacttaattg cccattttct gttaaagatt gttcaatcaa tgaaatcaaa tgaaactctt
    13441 ctactggaaa atcatacgag ccttcaaata ctggaatttc atggttttct gtgtagattt
    13501 ccaaggtaca ccagccaatt tccgctcgtg ccaatagctc ttcattttct cttttcaatt
    13561 gaataaatgc cttatctaat gcaatttttt tatctgttag taaagacatg ttatcaactt
    13621 ctgtaaatcg aatcatcatc attagtcaaa cacttcttcc accacttcta ccgcatacac
    13681 cacttgaata gggttattta aagaaaattt cacccgatat tcttgattgt tcaaactgac
    13741 gatatagcta tcctcatctt cagcataata ggtatattcg ttgcctttga cttgaaaata
    13801 tttttggatc gcttgtcttt gttgccattc gccaatcttt ggaaaatagg cgaaatgata
    13861 gccaaataaa acgcctatcc ctaataaaac aattcctgtt actataatca tctggtttcc
    13921 tcctgtacct gtttatcttc tattgactct tttgtcggta agcaataaag aaccagtaac
    13981 acaagagagc caatcgttgg gacaaaatag aaaaaaataa atgcccaatg aaaacctgcg
    14041 tctcgtaaac gtcgaacgct gagagaaaga gacgggacaa gaactacgaa acaatacaca
    14101 atcaatacgc ctatcaaaca accaattatt gtttcagaag cattcaacgc tcctgctagt
    14161 ccaatcgcta agcctaggcc aaacccaatg agggcattca ttaacgcaac gagccaatac
    14221 tcttttcttg tggctttccc tttgtaatta aagctattct gccaaaattt tttataattt
    14281 gtgatcatat ctttgtcctt tctaatgttt atttttgatt ctagtttctt ctgttttact
    14341 aacaatgaaa cttgccataa aaaaggtggc caatacacca ccagctagaa atccacttac
    14401 aaataacaaa attttttcct cctttctaaa aaatttcgcc ataaaaaggc aaagtatgaa
    14461 cacgataaat accatgtaca tactttgcct
//
LOCUS       DACDEG010000063        14339 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.065, whole genome shotgun sequence.
ACCESSION   DACDEG010000063 DACDEG010000000
VERSION     DACDEG010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.065"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(91..432)
                     /locus_tag="IV142_001900"
     CDS             complement(91..432)
                     /locus_tag="IV142_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8485555.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(432..662)
                     /locus_tag="IV142_001901"
     CDS             complement(432..662)
                     /locus_tag="IV142_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8485556.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            949..1287
                     /locus_tag="IV142_001902"
     CDS             949..1287
                     /locus_tag="IV142_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485557.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(1492..2067)
                     /locus_tag="IV142_001903"
     CDS             complement(1492..2067)
                     /locus_tag="IV142_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP8485558.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2676..3257)
                     /locus_tag="IV142_001904"
     CDS             complement(2676..3257)
                     /locus_tag="IV142_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8485559.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(3440..4066)
                     /locus_tag="IV142_001905"
     CDS             complement(3440..4066)
                     /locus_tag="IV142_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8485560.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LIESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4088..5419)
                     /locus_tag="IV142_001906"
     CDS             complement(4088..5419)
                     /locus_tag="IV142_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP8485561.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(5574..5975)
                     /locus_tag="IV142_001907"
     CDS             complement(5574..5975)
                     /locus_tag="IV142_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP8485562.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(6061..6267)
                     /locus_tag="IV142_001908"
     CDS             complement(6061..6267)
                     /locus_tag="IV142_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485563.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(6362..7864)
                     /locus_tag="IV142_001909"
     CDS             complement(6362..7864)
                     /locus_tag="IV142_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP8485564.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7882..8337)
                     /gene="argR"
                     /locus_tag="IV142_001910"
     CDS             complement(7882..8337)
                     /gene="argR"
                     /locus_tag="IV142_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP8485565.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(8435..9052)
                     /locus_tag="IV142_001911"
     CDS             complement(8435..9052)
                     /locus_tag="IV142_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485566.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            9273..10646
                     /locus_tag="IV142_001912"
     CDS             9273..10646
                     /locus_tag="IV142_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8485567.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10721..11833
                     /locus_tag="IV142_001913"
     CDS             10721..11833
                     /locus_tag="IV142_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP8485568.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFEEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11905..13542)
                     /locus_tag="IV142_001914"
     CDS             complement(11905..13542)
                     /locus_tag="IV142_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP8485569.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLLDEAKESLENLIQKLN"
     gene            complement(13710..14318)
                     /locus_tag="IV142_001915"
     CDS             complement(13710..14318)
                     /locus_tag="IV142_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8485570.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3894 a         2994 c         2539 g         4912 t
ORIGIN      
        1 acactagtag aatgaacgac atttacttgg ccttttttta atttcgttat gtttagcata
       61 taaaaatatt atagagttta cttacaatgc ttaaaacata tactgtataa tctgatcaat
      121 tttagccatg tcttgcaatg gtaagttttc tatttttttg agttttcgtt ccttaaaatc
      181 aagagatttt aattgtgtaa taattacttg tccttttata tctagctctt caggtaatga
      241 gtaacgtgtc ggtaaattct ttatagtgga agtaattgga catatgaccg aaaacattgt
      301 tttacggtta aattcataac gagagacgac tagtcctggt cgtctttttt gaatttcttt
      361 tcctacggag ggatcaaaat caatccatac aatatcacct tttttaggaa tataaattct
      421 ttcttctgac actataaaat ttctctccct tcaggagtta aatcttccca ttcgtctttc
      481 tcataatact cggcctcttc cccaccacta aatggatctt caatttttgg aactaacgta
      541 attgttccat catcagaata cacaacgata tattcttggt tttctgaagg tttctttccg
      601 ttatcagaag gtaaagttac cactacagaa cttccttgta atctagattt tgtaacaagc
      661 attatcatca ctcccttaat aaaagtatac tatataacac taagtaacgc aaaattcccc
      721 tttgtaaact atctagaaaa ttgttttgtt taaagtatat cttattggat ataaatagaa
      781 ataaattgta gccagttcat tttttcttta actaaaaaaa taatccaaga aacattgaac
      841 ctatggatcc aaaagctacc caaatactat agctaacccc ccgctacctt caattttgca
      901 gataaaacgc tttgggagaa aatttcaaag acaacaggcc atcctatcat gcccgattat
      961 aaacaaaaag ctggctattc cttttgctct gacaaatttc aattgggaaa gttcacgaaa
     1021 ctagcaggtg aagaagtttc aactgttcgg atcaaagaat gcccgcttca aatagaaaca
     1081 ttggttcaac gaatttcaac tagacaagac tttgctattg tagaacgtga gatcgtagct
     1141 atttttgtag aagaagggat tttacatgat ccaactcata tcaatgtgga taaatggcaa
     1201 cctcttatct ataaattcag agaatataca actgccaacc agtcgttaac tttgaatttt
     1261 agattccaag aattcaaaga gtcataatga taaacatcgt taggtataaa taaaaaagac
     1321 tcgcagcacg agtaaaaaac aaatagaagc tatacattag aaaggtcgac tgtgacccta
     1381 tagatgcagt atatgcagct tatgagcatt tcttctgttc tctttgtttt tgggatctgt
     1441 gctgaagtct ttttcgtttt ttgccgctct ttctttgaaa aatcgattat gttatacctt
     1501 ctccattact aattcaggca tctgctgagt aatgattttt cgcgcaaaag aaatatctgt
     1561 tacccaaggt tcgatttgtt cttcagtcaa gacaagcggc attcggtcat ggattggtgc
     1621 gactgtgcgg ttaggttcag tagtcattat gattgattct gccatttttt catcctgatg
     1681 gctacgataa aaaccgccaa tatacactat tttttcctta tttgaaaaca taaatttgtc
     1741 tttttcttca ttccactcat aaaagccact cattgggaaa acacaacgat tttgcaaaaa
     1801 tggttgatga aaggtctttt tttcttctac tgtctctgca cgggcattaa tcatcaattg
     1861 tcctttttta aatccggtaa atccccactt catgatttcc ggagtgagga tgttttcttc
     1921 ctcttttctt aacgtcacaa caagatttga cggaaaaact tccccagctg ctaattctgc
     1981 tgcctgcttc ggttgtttcg tctgggcttc tcgataataa ttctgaagga gctctgactc
     2041 tagatcaaaa aaataacgac cacacatcct aatccctccc aatctttttt tctatcatac
     2101 actgcaacaa gaagttcgca ccaacaaaac gctttcaatt tttgtagaac taaaaaagtt
     2161 gtgacaagtg acaaactctc cagtgaatgg tattctcctg ccaactcctc ggtcacaagt
     2221 cacaatattc aacaaacatg agaagctgct ttttgaaatt gttccttgtg actcggagtg
     2281 aaacggcagg cttacgacct ctgggtaaac ggtgttcaaa agcttgcatc tgcggtaata
     2341 agcccaacca aacgaaaaat atgagcagct atttttattt ggctgttctt attacttgat
     2401 gcagggcagc tttttcacaa cctctccact aaacggtgtt caatcagcta accttcggta
     2461 ttagctcaaa aaacggcaaa atatgaggaa ctattttgcc gtttgtttct ctaatactca
     2521 ggtcaaagcg ctgatttcac aacctctaat acacggtgtt ccagaagtag cttcttcgaa
     2581 aaataagccg aaatttcaca aaaatttgag aagcaatttt cgtaaattcc ttcttatttc
     2641 ttgaagctga ccacttctgt cacaacctct aattattaaa gaaccttttg cgaagttaac
     2701 aatctttctt tgatcaattg aaaattctgc ctaagtagtg atagatcctg tgtgactttc
     2761 aacaataaga tacttccctg gatttcagca atcaacgctt tggcttgacg gtctgcttct
     2821 tcatcggaca gtttttcttc agaaagtgcc aggatatttt gtttcgtata cttgacccat
     2881 tgattgacta attctagtag caacagacgg tattcttcat ccactgtact caactcaata
     2941 atcaaattac ctacaggaca gccatgataa gtgatctgtg tctcatgatc agcgaaaatc
     3001 cagtccaaca tttcttctaa tgcttcttct ggctgttttc cagcatttaa gatgtcttcg
     3061 aacaattctg tctgccatac gtccatatgc gcatggatca ccgctagaca aacttctttt
     3121 tttgaagaaa agtaataata aaattgacct tttcctgtat cagaagctgc tagaatatct
     3181 cggattgacg tttcatgata gcctttttta taaataagat ctttggtcgc ttccaagatc
     3241 ttttcctttg ttttcaatga tattctcctt tttcatttct catagtccta tattatacag
     3301 taaattcatc attttttctt cttaatcgtt taaaaaagac tgtgccaaat tatctgacac
     3361 agccctcact ataaaactgt tctcatatag ctagatgttc attgattcaa gaactgcgag
     3421 gacaaaagca attcctatct tatgatagtt gctctaatac ttcctctgtt gtcatcactt
     3481 gatctgcaat aaagccaaag ttcgtcaacg ctgcatccat tgcttcttgg ccaggagcac
     3541 caattgcatc tgtcgctacc acaacattga atccactctc tatcaattca cgcatatggg
     3601 aatcgctaca caagtttccg ttcattccac caagaattac tgtgtcgatt ccattttttc
     3661 tcaattggag cgccaaatca ttgctttctg gtccaaagat tttatgagga gagctgatga
     3721 tcgtattttc atccaaataa ggtttcaatt cttcgataaa atcggctcct gaaccttctt
     3781 ccactgattc atattgactt tttctagcaa acatctgctg ctcaagcatc atatgttctc
     3841 ctgcaccgcc aaattgccat ttatggtcat gtgggtaaaa ataatgagga gaaataaaga
     3901 gtttgtatcc tttttctttt gcttgtttta ccatttcaat catattttcg atcgtattgt
     3961 atttttctaa gatatctttt gtcaaaccaa agccaccgcc tgctggtttc aagaactcga
     4021 cttgcgggtc agtgagaact actgctgtat ttttattatt gaacatggtg gattctccat
     4081 tcgttattta ttttaataca tcatttagag cttcactcat tgtcggatga gaatagattt
     4141 gatctctcaa taacgtataa ggaagttttc cattcatagc tagcgaaatc aagttgatga
     4201 cttcatgaga atcttccgca tagatacttg cccctagaat caagtcagtt tcagggtcga
     4261 ctaaaacttt gaatacacct ttaggatctt ctaatacttg tgctttaggg acaccgcttg
     4321 tcatatactt gaataatttg taattgacgc cttgtgcttt ggcttgtttt tctgttaaac
     4381 ctacattgga taaagtcgga gtgataaata cactgtaagg cacagctttc cggttattcg
     4441 ttgttcgttt attctcgccc ttcaactgat ccataatgat tcgataatca tctagagaga
     4501 tataagtgaa ctgcaatccg cctttgacgt ctccaatcgc ccaaatgttg tctgcatctg
     4561 tacgaaggta ttcatccaca gcgattgctc cgcgatctgt tagtttaaca tctgtatttt
     4621 ctaatccaag tgatgctgta cttggttttc ttccagttgc agccaaaact tttgacgctt
     4681 ggattgttac ttcttggcca tcttctgtaa aggtgacggc agctgttgat tcttggtctg
     4741 ctacttgatc tacagataca cctagatgga attcgattcc cgcatttgtc atgtcttcta
     4801 aaatcatttg cgcgatatct tcatcttcac gtggaagaaa ttcagaatgg gcatcaagaa
     4861 cgacaacttt agatccgtat tcattgaaca ttgatgcaaa ttccaagcca atatatccag
     4921 ccccgatgat cactaatttt tccggtaatt gttcctgatc catcgcttgc gtagaatcaa
     4981 tcaagtagcg acttgtattc aatcctttga ttggtaaaat gattggttca gaaccagtat
     5041 tgatgaaaat ccgttcgcct tcaacaatca ctttttcacc attattcgtc acttcgattt
     5101 gatggttaga aagaaaacgt gctgtaccgt ccatcactgt cccagttgcc tcatcgctga
     5161 tcatatgata atttttggcg cgaagcatgc ccgtcaattt ttctttccga gcaacagcct
     5221 ctgtaaagtc gattcctctt tctccattga aaatcaatga tttagaagga atacagccga
     5281 tgttgataca tgttccgcca tacatacgtg ttgacttttc aacgactaaa accgattcac
     5341 cttttgaagc taacgccttc gctaaagtct taccgccctt tccaaaaccg atcacaatat
     5401 tttgatattt ttccatcatt tttccctcgc ttctttttta cctaccgatt ggtacaaaaa
     5461 tatgataacc gataattgtc tattctccaa atcatctgct ttatttgtca aaaaaaacca
     5521 gacaaaaagc ggtcctttca cgtttttagt ctggttcgat ttcctttatt cttctaaaca
     5581 atcgttactt ctacatcgat atttcctcta gttgctttgg agtaagggca gaattcatgc
     5641 gcatcttctg caattttttg ggcttcttct tcgctgatgc catcgatatg cacttctaat
     5701 actacaccga tctgatagcc gccatcatct aaattataaa gagagacacg tgcttttact
     5761 gtggactctg ctgtcattcc tgcttctttg atcatatgtt gtaccgcccc attgaaacaa
     5821 gaagcataac ctgctgcaaa taattgttct ggatttgttc cttctgcatg gattcctgga
     5881 ggagtgattt tcatatgcat cgagccattt ggtgcctcga cttctccatt gcgcccgcca
     5941 tggttgatca ttgctgtttc gtatgctttt ttcattgtac tgctcctttc gattgaccat
     6001 ttgctacttg gctataatta tagtgtatga caagtaaggc gctttctaca aaaattctgc
     6061 ttattcgaat accagaatgc cgtcttctac tatttttcca aaaaacaaaa aaatactact
     6121 cgtgaacaac agcgtcatat ttgttccaaa ataaatagca atcttttcta cataatcttt
     6181 tatatgattg attttaagat acatatatac cagaaagttg accccataaa ccaatactgt
     6241 taaaaaaata accatattcg ttaccatttt ttcctccttt tgctggtctc cctaccagca
     6301 ctcgatttaa ccataccact ttttttgccg tccaagcaac tagcctttca aaaagactgg
     6361 tttattctgc tgttaatgat tcaagatcag aaaaatgtcc gaataatttt gctaatttta
     6421 ctaacgccaa cttgttcttt ccgaattcat cattttctat ctgtaatact agtaacggct
     6481 catgtaagct taaacggatc aaaaaccagc cgtttccgta tggctcttct agatcatatc
     6541 gaataccttc ttcattttcc gggtcgaagt tcatcccttt ttcttcttcc aacagtcgct
     6601 tcatttcttc taatatcttg tttccttgtt cgcgtggttc atcgctgaac aattttaatc
     6661 ggatttcttg tacttctacg ggctgtttca actctttgat ttgttcatct aatgtgatac
     6721 cttttctttt taattctggc aaaagcatca ggatctttgc gatgacatat gcaccatcat
     6781 ccagaaaata gttttccttg aatgcagcgt gtccgcttgt ttcgatggct agctgtgtat
     6841 ctttcccaag acggttaagt tctaatgctt tattgattac atttcgatag ccggaaatat
     6901 aacgaacttg gatgcctcct ttttcttcaa tgaatctttg tagatgagaa gaagtagggg
     6961 aattcgtcac gattcttgtt cctggatgct cttttaatac gattgtactt aatactgcga
     7021 tcaaattatt tcgattgatc actgttccag aagcactgac aactgcagaa cgatccacat
     7081 ctgtatcgaa aatgatccca agatctgctt catttgctaa aacagcctgc ttgatacttt
     7141 ccatcgcttc tttattatca ggatttggga tatgattagg aaatcggcca tctggttcca
     7201 aaaattggga acctgttgaa tccgcaccta agctattcaa tactttttca gcaaagaatc
     7261 caccagcacc atttccggca tcaacaatga ttttccaacc atttagtggt ttgctttcta
     7321 gtgttccaat ccccttttta atttttgtga ctaaatcttg cgcataaggt gtcaaaaggt
     7381 ctgctttttc tacgcttcca gtttgttcgc tttttatttt ttgctgctgt gataggataa
     7441 aagcaatatc ttctttttct gctcctcctt ctgaggtaaa gaattttatt ccattataat
     7501 aatagggaag atggctagca gtcagcataa ccgcagcatc gcacgagaac tgttcaaatt
     7561 gagtggacat aaacatagca ggcgtagtag ctaattcgaa atcgatgaca tgtatccctt
     7621 gttctttcag actttggatc aacgcctctt tcagtgctgg tccgcttatc ctactgtcat
     7681 gaccaatccc aatcgtccat tttttatttg gatcggttga tttcttcttt ttctttatcc
     7741 agtttgctag tccccagccg atctttttta ccatgtctgt cgtcaagttt acttcatatc
     7801 cttcaactgg taatgcgatt cctcgaatat ccgatccatt ttgcagcgct ttcatatctt
     7861 tcatataaaa gtcctcctca tttattccct cattttttcc agcttttctg caaatcgctg
     7921 tgcttcttct tctgagcggc aaataataat gaatgtatct acaccggcca tcgttccgac
     7981 aacttcaggg taagccactt catctagcca attcgttacc acatcggcac catttcgttc
     8041 agtcaatacg ataaccatga actgaacaga ctggatacgc aaaacctcgc gtctaattgc
     8101 tgtacgcaaa cgttcttcat ttaaagaaac agatggctgg ttaaataatg tatagcgtac
     8161 tattttgttt tcattttgac tttttactaa cttcatttct cgaatgtctc gagaaatcgt
     8221 tgcttgagtc acatcggcgc ctgcttcatg cagccgcgca agcagctctt cctgtgtccc
     8281 aataggttgt tctgtaatga tctggcggat cagtgcttgt cgctgtttct ttttcatatt
     8341 agacacctct caatgttgat tatagcaaaa gataagtaat atttcttcat attttggtat
     8401 tttttacttt tattttttgt tttcattcag gttgctaacc tttttctatt ttaacacgca
     8461 aaatcgtctt cattttttca ggtgctgatt ttcttggatc gctgatgtaa atctctttgt
     8521 gttctttaga agctctttca tagccttctt ttgcgatgaa tgcttctaat ttctcaaacg
     8581 ttgcaggttc ttcggcatag ggcccgacgt gtaagatttg ggcacaaaga ccttcttcca
     8641 tctttttgaa tactacttgt tctttcaagt cttccggaag tttgctagaa cgctgaatgg
     8701 cttcttgtgc tatcgcttct gtaacaaagt caggctgtcg gatcatcaat tgataagaaa
     8761 aatgttcttt tttcatcact ggttcatgca aatacttttc ttgcagtccc cattgtcctt
     8821 ccagtggata gacagtatac atctgataat caggaatcag ccaatttttt ttaggcgaca
     8881 tgcgtattgc atagctgact gcatatagac aggctactct tcgtttaaag tcttcctgat
     8941 tcgggtcgcc gatccctctg atgctaaaga atggtttttt aggaacctct agtatttgcg
     9001 ggcttttttt catactatag ctcttgtctt tacgccattc aattttttcc atttttcttc
     9061 ctccgcgaaa ttccatttag ttatttatta tcatacctct ttttgagtga cttggctaat
     9121 cgtgttgaca ctatttttca gtttgtgcta gaataagtga agtaaataaa tcgtacagat
     9181 aacacgtttc ggcgtgtcga gagagaggcg gtttctattt ttataataga aatcactctc
     9241 tttttttacg aaatttttta gaggtgatgt aaatgacaaa acaaaaacag ctgcttttag
     9301 tacctgcagt aattgcaaca gggatgatgt cttttgcggg cgtattgatc gagacggcca
     9361 tgaatgtaac tttcccaact cttatcagac agtttgattt aacgactgca caagtccaat
     9421 gggtaacaac gatttatctt ttgatgattt cgatcattgt tccgctgtct acttatttga
     9481 atcgcaattt tagtctcaaa aaactctttt tagcttcaaa tctgcttttc ttggcaggta
     9541 taatgatcga tttcttctct cctaccttca tggtcctatt gtttggtcga ttgctccaag
     9601 gtgcagcgac aggtatcgct cttccgttga tgttccatat tatcttgaca tttgcccccc
     9661 tcaatcgacg aggcgcgatg atgggtgtcg gtactttgac aacggccatt gctccagcaa
     9721 ttggccccac ttatggaggc attatgactt cacattttac atggaatcat atcttcctgt
     9781 ttcttgtgcc cgttttgatt ctttctttga ttatcggctg gtttgctatt cccgagatgg
     9841 cagtacaaaa aagtggttat ttggattgga taagcgtttt aggaatttgt cttttgttta
     9901 gtggaagtct aacatttttg agtacgttgg gaacagtttc cagctggatt gctttaatca
     9961 tcggcggtat tggctggttc cttttttact atcgaagtaa aaaagtggag catccactga
    10021 tccgactgtc tattctttca aataaaacat tccgcttgtt tttgattggc ttcttagttt
    10081 atcagttttt attacttggt gtatcttttg tcttgccgaa tttccttcag atcgttcaag
    10141 gaagtagtcc ttttgtcgct ggattagcga tgcttcccgg cgcagcggtt ggtgctttgc
    10201 tttctccttt ttccgggaaa atgctggatc aatatggtcc gaagaagcca atttttgctg
    10261 ggctgattct ttctttgatt ggttggctag cattattcct actcatagga cacgcttcag
    10321 tgggtgtatt gattgcttgt catgtgtttt atatgatcgg tatcggatta tcttacagca
    10381 atatgatgac caccggaatg aatgcactga atgaagaact acaaggagac gggaacgcaa
    10441 tgttcaatac cttgcagcag ttttctggcg ctgtggctac ttcattagta gcggtcatta
    10501 tcaattacgt ccagcatcat acttcccata actatgaagt gtctactaca ttaggatcaa
    10561 aagcagcatt aggcgtcttg ttgctgttgc ttctcgtaag ctttgctcga ttcgcctatt
    10621 atctgttctt tgctaaaaaa gcgtagattc catatacggc aggtgttgca tctgccgttt
    10681 ttttgtgtga aaatggttgt ttgtaaaagg aggctttttt atggaaaaaa ttgccattat
    10741 cggtggcggg atcattggta tgaccctagc cagttactta gatacatcaa aattcaatgt
    10801 tgctttattt gatgaaggga caggacaggc aactaaagct agcgctggga ttatctcccc
    10861 ttggttgtcg aaacgcagaa acaaacaatg gtatcgtctg gctaaagacg gcgctgcttt
    10921 tttccctaaa cttataaaag atttccaact tgatcaaacg atttacgcac aatcagggac
    10981 acttttgctt cgacctgaaa aagatttagc tgaattggct gttcttgccg agaaacgaaa
    11041 agaagaagcg ccagaaattg gaaacatcga atggctttca cctgaacaga catctcaagc
    11101 acttccgtta ttgaaaccag cagtttcact gcgtatcagt ggtggtggtc gtctggatgg
    11161 aaaagcatat ttagaacatc ttggtcagcg tttagcacaa aaacaaatgg agcagatcca
    11221 ggcaaaagtc actttgcata agaagagcga ttcttggctc atcaagggac cgtttgaaga
    11281 atacacagct gatcgtgtaa ttttgacacc tggcccggca cttaagcctt tattacaaga
    11341 attaggctat gctgcagata tacgtccgca aaaagggcag ctcttagcat ttcagacatc
    11401 ttataagatg agtcaagact ggcctgttgc tatgcttgac ggagaagctg atttcattcc
    11461 tttcatggat ggaaagatcc ttttaggtgc tacccatgaa aacgatcaag gctgggattt
    11521 ggctcctact agcgaagctt ataagggatt gactaagagt acagaacagt tcttgactga
    11581 tccagatcag ataaaaaact ggccatacga gttccgtgta ggtacccgtg cttatacgtc
    11641 agatttttcc ccattttttg gcgtactgcc agatgatcca agcttactgg ctgctagcgg
    11701 tctaggctct tctggcttga ccacaggtcc ttatatcggc tatctattag ccaacttctt
    11761 aaatactggt cattggtcta cggaagatta tcaaaaggac atctccacct atattacgaa
    11821 aaatccatca tagaaaaaca gctttttcaa gtaaggatcc cgagcgatat tgcgcatgga
    11881 ttacttcttg aaaaagctgt tattttagtt caatttctga atcaaattct ccaaactttc
    11941 ttttgcttca tctagcaaga gatcttttcc tatctgagct tcaatgaatc gtaaacgaat
    12001 atcaagtcgt tgtttcacca gatttttata ggacgtatcc tcaaaagaaa caggaaaatc
    12061 tggtataggt aaatagtgga tgtcttcaaa tggaccaaac gacttgaatt ctgccgtttg
    12121 ttgcacgatt ctttcaacga ttgacaatgt cgtttcaggt ttgatttttt tatcatcgaa
    12181 atatccagta ttcaggacat agcaggctgt ttgctgtttc aaaaacaact ctcgaaaagc
    12241 ttgatagtct tccctaagag gatagacacg gaaaggatta gcaaatggtt caatgactag
    12301 ctcatctagc tgtctttttg tagtgatatt ttccgcgtct gatcgttttg ttgctaatgt
    12361 tgcaccaaaa gacgctgcta aaactgggtc attgatcttg ataattggcg gaagcgaatc
    12421 atctttcatg atccaaaaaa cagcatcgat cttttcgcta agatgatcga ctcggtcggg
    12481 tgtcgcataa cgtgatttga ctgtccgccc atttccgttt cgtatatctc ctgttactag
    12541 gactttttct cctttacttg tacatgtcac gcccacattc tgacaagtaa ggaaagaaga
    12601 aatggccgga tcatcagacg gatagtcttg tgttttatca aagtaagaag gttccaaggc
    12661 agtagcaaaa ccattttttc gagaaataac gaaagcatcg tcatgcaaga tcgaaacaga
    12721 gtgaccttcc gtatgttttg ctaaggtaat cgttgatttt ccagaaccgg acaacccaaa
    12781 tacagccatc gtataggtct ggtcattact agcatattgt ttcatcccac cgtggcaggc
    12841 aataaagcca ttgcgatttc ccaatgccca agcaagcgtc aaagtcgctt tttttaattc
    12901 tccaaagtaa cgtaatccta gcacagccgc tgtattatgg agagcatcaa acaaaatgag
    12961 tccatttgga aatgtttcat gtgtccattc gggatcacaa aagatataaa gatctccctc
    13021 atcaaaggaa acagactttt gataagtctc taagatcttc gaattcgctg gctgaaaatt
    13081 cagcaaataa gaatacaaat tgttctcaaa tccttctgga atcagcaaat gactttgcac
    13141 agaaaattct tcagataggc caacgattac ttttgtatgg taaaactggc gcttgcgact
    13201 ttgatacaca gcttctctta aaattttctc gtatttgctt tgttccttgt ctaagtcctc
    13261 aaagatccgc cgagcacttg ctgttcttcc cacaatgctt ccatcattga atactagtgt
    13321 cctaacatca gctggtaaat ctagttcttc agcatgtgag attgttaaat ctgtgatgat
    13381 cgttcctgga ctcccttttg ctagttgata agctgtcttc ctgtcaaaaa cttcatgaac
    13441 attattaccg taaaatgtcg tttctacaat cgatcgaata ctggataaca gtggatttcc
    13501 acttgtgatt tcgtttaatt cgagactctc ttttgtactc atggctcctc ctcattccct
    13561 aaacaaaaaa acgttttctt tattatacat tattcgagtt tttatttgta ctgctgcgct
    13621 gtgggaagca aacgagttat ttttcacaca aaaaaaccga gaacttgaat aaacaagttt
    13681 ctcggtttca tcattttcct tttatcggct tagtaccagc cgtttgctaa ccagaatgtt
    13741 ttagcagcat cccatgaacc ataacgagat gttacatatt gttctgctac acgttcttga
    13801 ttggcagctg aatagtcgcc gtttaagtat gaagcatcta attgataacg gccgatgtaa
    13861 cgtccgttag tagctgtgta tgaaccgcct gattcttttt gtgcgatcca ttcttttgca
    13921 gagcttgaag ttggcgctgt tgcagcagct ggggcagcag gagctacttc aactgtttca
    13981 acaggtgcaa cgtatgtatg ttcttgcgtt gctgtttcag ttgtagatgc tgtattttct
    14041 gttgttttgt tttctgttgt tgctgaagaa tcatttgaag ttgggattgt taattgttca
    14101 ccaacataaa tgcggttgat gtttgcgatc gaattttttt cagcaattgc atcgatcatt
    14161 gagttgtctc ctgcaaattt ttgtgagatc ttagataatg aatcacctga ttttactgta
    14221 taaacttcat cagcgtgagc atttgtagtt cctacaaaca ttgcagtagc accagcagca
    14281 aaagttgtac ctaaaacaat tttcttaaat gaattcatat taaactcctt tttttctcc
//
LOCUS       DACDEG010000064        14111 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.066, whole genome shotgun sequence.
ACCESSION   DACDEG010000064 DACDEG010000000
VERSION     DACDEG010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.066"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..921
                     /locus_tag="IV142_001916"
     CDS             <1..921
                     /locus_tag="IV142_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8485571.1"
                     /translation="SKKQVAFNTYVGYKHYVHNRIYKFFYPKKIKLNRLKPYHLQEFY
                     QYMLNDNCTANTVIHYHAFIRKALQEAVITELISTNVADKVRKPKKKQFVSQVYNQQE
                     ILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGLKWSAIDFLNKKIMINHVIIENPENM
                     SQLIKKDQTKNSSSYRTLPLVETIERALVNQAKWQQDNRILLGDDYILEDNEYVFTME
                     DGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRHSNASIMLDSGQNMKSIQEWLGHASY
                     STTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            1495..2064
                     /locus_tag="IV142_001917"
     CDS             1495..2064
                     /locus_tag="IV142_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP8485572.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            2051..2911
                     /locus_tag="IV142_001918"
     CDS             2051..2911
                     /locus_tag="IV142_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8485573.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            3101..3805
                     /locus_tag="IV142_001919"
     CDS             3101..3805
                     /locus_tag="IV142_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8485574.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3810..5645
                     /gene="walK"
                     /locus_tag="IV142_001920"
     CDS             3810..5645
                     /gene="walK"
                     /locus_tag="IV142_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP8485575.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            5642..6958
                     /locus_tag="IV142_001921"
     CDS             5642..6958
                     /locus_tag="IV142_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485576.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6959..7828
                     /locus_tag="IV142_001922"
     CDS             6959..7828
                     /locus_tag="IV142_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485577.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7883..8692
                     /locus_tag="IV142_001923"
     CDS             7883..8692
                     /locus_tag="IV142_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8485578.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(8795..9424)
                     /locus_tag="IV142_001924"
     CDS             complement(8795..9424)
                     /locus_tag="IV142_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAP8485579.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            9594..10886
                     /locus_tag="IV142_001925"
     CDS             9594..10886
                     /locus_tag="IV142_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8485580.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10962..11624)
                     /locus_tag="IV142_001926"
     CDS             complement(10962..11624)
                     /locus_tag="IV142_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8485581.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11849..12133
                     /locus_tag="IV142_001927"
     CDS             11849..12133
                     /locus_tag="IV142_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP8485582.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            12184..13809
                     /gene="groL"
                     /locus_tag="IV142_001928"
     CDS             12184..13809
                     /gene="groL"
                     /locus_tag="IV142_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP8485583.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4751 a         2382 c         2987 g         3991 t
ORIGIN      
        1 tcaaaaaaac aagtagcatt taacacatat gttgggtata agcattacgt ccataatcga
       61 atttacaagt ttttttatcc taaaaaaatt aaactaaata ggttaaagcc ttatcattta
      121 caagagtttt atcaatatat gttaaatgat aactgtacag caaatacggt tattcattat
      181 cacgcattta tacgcaaagc tcttcaagag gctgttatta cagaattgat aagtacaaat
      241 gtagcagata aagtaagaaa gccaaaaaag aaacaatttg tttctcaagt ttataatcaa
      301 caagagatct tgaaattact tgacattttg tcagaagaaa aactctattt ggtagttctt
      361 ttaacagcat tttatggatt gcgaagaagt gaagttttag gattaaaatg gtctgctatt
      421 gattttttaa acaaaaaaat catgataaac catgtaatca tagaaaatcc agagaatatg
      481 tcacagttaa taaagaaaga tcaaacaaag aactcttcca gctatcgtac attacctttg
      541 gttgaaacga ttgaacgagc attagtaaat caagctaaat ggcaacaaga taatcgtatt
      601 cttttaggag atgattatat tttagaagat aatgagtacg tatttactat ggaagatgga
      661 cggttgatga agcctcaata tgtaacgcat cgtttgagta agttgattaa gaaaaatgga
      721 ttgaaaaaaa ttcgctttca tgacttgcgt cattctaatg cttcaataat gttagatagt
      781 ggccagaata tgaaatccat tcaagaatgg ctgggacatg catcgtattc gacaactgct
      841 aatttataca cgcatttaac cgcaggagta aaagatcgtg cagcagaagc attagaaaac
      901 gcatttgggt ttaaagaata aaaaaacaaa aaacaatatc tttggggtat cgcttcttgt
      961 ttttagaatt tttttgttgg caatttgttg gcagtatgtt ggcaatgaaa aaattcacaa
     1021 actaaaaacc tatttataaa gctttataaa acttttttca caaatagagc atttggttaa
     1081 gagtcagctg ctctaccaac tgagctaacg gcccgtacaa aaagtactta ataatagtag
     1141 cactaagaat tttaaaatgc aagactacat ttaaagaaaa tcagaaaaat ctttacttcc
     1201 ttataggaaa tcattcatag attattcaca tttgctcgct attatataag cataccaaca
     1261 aacaaagttc ttgcaagaga acaaacccta acactttttt cattttttac tcctttcccg
     1321 ccgtctcccc ggcgggtctc ctgttcttaa cgataaaaga caagaaggct tagccttctt
     1381 gttttttgtt ttcatggaat cccgctaatc aaatcaccta gttaaagcaa ctagattgaa
     1441 tgtttatcat aagtctgata tcatgtttat gaaatgaaaa aaggagcggt tatgatggaa
     1501 aaaatgcgtc agcaattaat ggagtggggg aaggaactag aaacattccg tctgcctcac
     1561 tgggaagaac tccctgactt agaactttat atggaccaag tcagaacgtt agttgatcgt
     1621 tatctttcac ctgtgataca aggagaaaag catccattgc ttacctcttc tatggtaaac
     1681 aattacgtta aactaggttt gataccagca ccagttaaaa aaagatataa caaagaacac
     1741 gtagcatttt tacttgcaat cactacgttg aaacaagtat tgactattcc tgaaatcaaa
     1801 gaagggatat tgttccaagg aaaaacagta ggaattcgtg aagcctataa tctattttgc
     1861 gatgaacaag aagcagctgt gtggatggtc agccaattag cacaaggtaa atctcatccg
     1921 caaaaattcg accaggcaac ccctgtagaa tatattgctg taagagcggc aactttatcg
     1981 tttgcaatga aacttcttgc ggaaaaaacg attgttctag aaaccgaata tttaaaagag
     2041 gagaaaacaa atgaaaaaca ataaaatcgc aattttagta gattcaggta cggatgtgcc
     2101 agaagaattg ataaaaaaat acgagatgta tatgatccca ttgaaaatta tttataaaga
     2161 tcaggtatat acagataaaa ttgacattac ctctgaagag atctatcaac gtttgccaga
     2221 ggaaatccct ggtacatctt tgccggatgg tgagtcgatc acagaaatct ttgagcaaat
     2281 caaaaaagac ggttatgaaa aggtacttgc ggtaacaatt tccagtggat tgagcgggac
     2341 atataatgtg gttcgtttaa ttgcacaaca gcaagaagaa ttggaagcat atgtactaga
     2401 tacgaaaaat attggtattg gtgcaggttt ttctgcgatc caagcagcaa aatggctgga
     2461 agaaggagta gaatgggatc aactgatcag taatctgaat gagttagtcg aaagaacaaa
     2521 agttttcttt aatgttgcga cattggaata tcttcaaaaa ggtggacgga tcggtttagt
     2581 tgcttctatt gtaggaaccg ctttaaagct taacccaatc atctcatgta atgaagaagg
     2641 gatttattac acagtaggaa aagctagggg acgtaaaaaa agtttggata aaacggttgc
     2701 ttacgtcaaa gaaaaaatcg gtaatgcaaa ggtcttcaac ttagctgttg cccatgggga
     2761 tgcgaaagaa gaagcaattg aaatggaagc gagacttaaa agagagttcc cacaagcaaa
     2821 ccaaatctac tttggccaga tttctccggc attagtagtc cacactggtc cgggattatt
     2881 gggtgtcggt gtacaagttt tagaaggata aatcaaaaaa aggaaacact gaggtgaaaa
     2941 tcagtgtttt ccttttttat tgaagttaga acaaaaaata ataaagtatt ttgcactttc
     3001 attttaattt tctcttttgt tttataggca aagatgaaac aaaagcagaa atacggtata
     3061 atgagaaggt atgacaataa tatataagag gtggctcttt atgaaaaaag ttttagttgt
     3121 cgatgatgag aagccgattt cggatatcgt caaattcaat ttagccaaag aaggctatga
     3181 tgtttatact gcatatgatg gggaagaagc cttggaaaaa gttgctgagg tagaacctga
     3241 tttgatttta cttgatttga tgctacctaa aatggacggc ttagaagttg ctcgtgaggt
     3301 acgcaaaact tatgatatgc cgatcattat ggtaacagcc aaagattctg aaattgataa
     3361 agtcctaggc ttagagttag gcgccgatga ttacgtgacc aagccatttt ccaaccgaga
     3421 attagttgca cgtgtcaaag caaatcttcg tcgcggtgcc acggctgcga aggaaccaga
     3481 agaggcagca cctgcggaat tgacgattgg tgatttgacg atccatccag aagcttacat
     3541 ggtgacgaaa cgaggagaaa caatcgagtt aacacatcgt gaattcgaac tactctttta
     3601 cttggcgaag caccttggac aagtgatgac aagagaacat ttattacaaa cggtttgggg
     3661 ttatgattat tttggagatg tacgaacagt ggatgtaact gtacgtcgtt tgcgtgaaaa
     3721 gatcgaagat aatccaagcc atccaaatta tttagtgaca cgtcgtggtg tcggttacta
     3781 tttgcgtaac ccagaacagg agtaattgga tgaaaagaaa agtccgcttt tttcgatcag
     3841 tcaattttaa gatcgctatt acctttattc tgattttatt gatctcgatc gaaattattg
     3901 gtgcctactt tatacgtggt ttagaacgtt ccacgatcaa tacatttatt aaagatatga
     3961 atcaaacggt ggaatcatta gcaacaacaa tcagcccgga attgaatcga aaagataatg
     4021 cagatgatga agaggtaaat gcgaatatca aaagatttat tgaaaacagt gcgactagcg
     4081 atatcatcga aatacgggtc gttgacgaaa aagggatcat ccgaggaaca accgatgtca
     4141 atgaacaaag cgctgtggga aagaaaaatg attatgttga tatcaatgat ttcacgacta
     4201 aacgttatgt tgctttagat aacgacgata aacgggtgaa tatcaatgtt caaccgatcc
     4261 tgtccccgac cggcgatgcc gttatcgggg ctctttacgt caaaagtaat atcgaacaaa
     4321 aatattcgga aatcaataac acagcagtca ttttctttac tgcctcacta attgcggcat
     4381 ttatttcaat gatcgtatct gttttagttg ctcgttcgat tacacagccg atcggtgaaa
     4441 tgagagaaca agccattcga atcgctagag gagactacag tcggaaagtc aaagtgcatg
     4501 gacaagatga acttggacag ctggctgata ccttcaatca gttagctgaa cgtattgaag
     4561 aagctcaaga tacaatggaa gctgaacgca atcgtttgga tagcgtgcta tcccatatga
     4621 cagacggcgt cattgcgaca gatcgcaggg gtaaagtcat tacgatcaat gatatggctg
     4681 tttccttgct agatatcaaa aacgaggaag caatcggaca atcgattttg acacttttag
     4741 atatcgaaga agaatatacg ttgcggaaat tattagaaga tccaaacgaa atggtacttg
     4801 accgttcaac cagtacattg gaaagtgatc agatgatctt gcgagtagac ttttctatga
     4861 tccgtcgcga atcaggtttt atcagcggat tagtagctgt cttgcatgat gtgacagaac
     4921 aagaaaaaac agaacgggaa cgtcgtgaat tcgtctctaa tgtttcccat gaacttcgta
     4981 caccgttgac aagtatgcgt agttatatcg aagcattgag cgaaggtgcg tggaaagacg
     5041 aggaagtagc accaaatttt ttgaaggtaa cgctagatga aacagaccga atgattcgaa
     5101 tgatcaatga cctcttaaat ctatctcgca tggatacagg taatacccag cttcaattag
     5161 aatatgtcaa tttcaacgag ttagtgaatt ttgttcttga ccggtttgat atgatggtgg
     5221 gaaatcagga aaaaaattat aagatccgtc gtgaatttac gcaaagagat ctttgggtcg
     5281 aagtagatac agataaaatc atccaagtag tcgataatat catgaataac gcgatcaagt
     5341 attcaccaga tgggggaacg attacttgtc gcttattaga aacgcataat aacgtggttt
     5401 taagtatcac cgatcaagga ttggggattc ctaaaaaaga tctgaaccga gtgttcgagc
     5461 ggttctatcg agtagataaa gcacgtgcaa gagcacaagg tgggacagga cttggattag
     5521 ctatttctag agaagtgatt aaggcgcatc gaggcgctat ttgggcagag agcaaggaag
     5581 gaaaagggtc gactttttat atctcgctgc catatgaacc ttatgaggag gaatggtggg
     5641 aatgagaatc tctgaaaaag tcgttcgtat aggactcatt ttgatggttg cgttaagcat
     5701 atacttctct tatgcaatct ggctgagccc agcaggaaaa agttcaatca acgtagatga
     5761 gacaaacact caaatgatcg agtctcaaag caatcgaaaa acttccgaag tctttcttcc
     5821 gcttcatgtt acatggcacc attcgggaat gatccaagaa accaacagtg aaaatctcgt
     5881 gtaccgattg caattgatca tcgataaagc aagatttgga aagctttctg aagtagtttc
     5941 aggagacaaa tcacagtttg aacaggtgaa aaatctgaac tctggttttg agttggccta
     6001 taatgcgcct tttttgctaa gagaatacaa ggaagcattt gatttagatt tagatgtgtc
     6061 gaacttggca aaagaaagcg aaaatatgta ttttactaga atacaattcg atcaaggaaa
     6121 taaaaaaatc cgatttttga attattcgaa ccatctcgta tatgaagcaa atatttcgtt
     6181 gaattggaca gatatcaata aagaattaac agcaaaagac acaaaatgga cagaaatgac
     6241 aacagaacca tcgacgatcg acacgcaata tgctacgaaa cattcgatca aactgaaaaa
     6301 gtatagttat atcctgtcac agcaacccta tacacttttt agaaatgctt ttttccagaa
     6361 accagatagc gtgaaaaata atgatgaaag caatgaattg attttttacg gtgggaatga
     6421 aacaatgtct atccattcgg aaacgcaagt agttgatttt cgtggagagc tgccagagaa
     6481 aaaagaaaat gctagtgtat tttctgaaag tttcccctat attagcaaat taggcagttc
     6541 tttagggaac ctcagatatt ttgaccgcag tgacaatacg atcgattatc ggatattcgt
     6601 ggaaggcttc ccggtttttg ggacagacag cagaggacag attggtgtgg aagttggcgg
     6661 tgaaacgaat ggttctgtgc aagtgaatat ccaaaccagt ttgaatacga ttcaagtgcc
     6721 gataccttca gacgaagaag tagaacttgc aagtacgaat gaaatcatcc agcagttaaa
     6781 agctaaggga gcagatagca acaagattgg ttcagtgatc gttggctata catggcaaaa
     6841 tgtcaaagaa acgaatcgtg tcgttgatct cttacctgaa tggtatgtga aatataaaga
     6901 taactggtat tcagctaacg accttctgga aaaattacct ggattggagg cgaattaatt
     6961 ggatttcaaa aaaatcgaat ggatattttt cgtcgctttt ttagggttga atgtgttttt
     7021 attcagcagt tatcatgacg cggtatacca agaaaacaat gtcattcgct ctaatcagac
     7081 tgaatcaatc caacaacgtc tggctagcga cgatatcact tacacgggga agatttcgtc
     7141 tgagaacaag caaggctatt atcttagcgc ggagcagacc aatcttagca cttcattagc
     7201 gaattatcgc aaggctggta aaagcggctt gctggcaagt ggtgtgtttt tagacggtga
     7261 tgttttgacg aaatctttat ccagttcaga tgctgaaaag aacgtgatcg atccagataa
     7321 aattagtagt tccttggaag attttttatc taacaaagac aatattttat tcggtaatga
     7381 ttatgtgtac acgaaaaact tttctcattt agaagaagaa ccactagaaa tccaagcaag
     7441 ccaagagtat gaaggcatcc cattcaatga tgatacagca aaaatcgttc tttctttaga
     7501 aaaaaacgat aatgattata gcatcacgaa atacatgcag acgcatttat ctgatatcga
     7561 gcagctgcgc gaaaagacgg aactgtatac ggaagaggaa gcaatcaata cattgtacat
     7621 caataacaaa ataccaagag gatcaaaaat tttatggcgt cagctagctt attcctgtat
     7681 tctgaaagtc cgggaaaaaa atgtgtatgt tcctgtttgg catgtagcga tcgaaacaag
     7741 cgataaagtg gtccaaattg agagtgtcaa cgctttcagc aatacgatcg tgacaaacaa
     7801 tactgtacca aaagtggaag atcaataaaa aagcgagtat aatgaaaaaa gtttacaaag
     7861 taagaaggga agtaggagtc aaatggctga agaaaaacca gcttttaaaa tcagtatcct
     7921 agcaagtgga agttcgggta attcacttta tatcgaaagt gaaaaaaaac ggcttttggt
     7981 cgatgcagga cttagcggta aaaaaatcac ttccttgatg gctcaaattg atcggacacc
     8041 ggaccaatta gatggaatac ttgtgacgca tgaacatcga gatcatatac atggcgtggg
     8101 tgtcttggct cgtaagtatc atttggatgt ttttgccaat cagcagactt gggaagcgat
     8161 ggaaccactg attggaaatg taccagtgga acaaaaacat ctatttgaga tggggaaagt
     8221 cagaacattc ggcgatatcg atattgagag tttcggcgtc tcccatgacg cagcggctcc
     8281 gcaattttat cgtttccata aagaaaatcg gtcattcgtc atgttgacgg acaccggata
     8341 ttgcagtgat tatatgcgag gtgtcattga aaatgccgat ggttatttga tggaaagtaa
     8401 tcatgatctt gagatgttac gcatgggacc ttatccgtgg aatttaaaac agcgtatttt
     8461 aggagataga ggacatcttt ccaacgaaga cggtgcactt gtcatgactg atgtgatcgg
     8521 taatcggacc aaacgaatct atcttggcca tcttagcaag gaaaacaata tgaaagaact
     8581 tgctcattta acaatggaaa atgttttgaa agaaaaagat tttggcgttg gacatgattt
     8641 tgaaatctat gatacagatc cagatgaagc aactgagcta ttttcgattt aaacagttca
     8701 atcaataaaa atataaaaag cagaagataa aatcaatcag ggttggtttt atcttctatt
     8761 tttatttttt tagcatatta aaaaaataac cgtattatcg actgactaca ctgatgtgct
     8821 gcttttcatg tttctcatcc agtatctcat ctactaatgc aatcgcgtaa tctgccatgc
     8881 tgatcgtact gtcgccagca gcgtttcttt gtaaataatc atcacttaac aaataagtac
     8941 ctgtacgttt gccatgtgga tcaaataaag aagctggact gatataagtc caatgtagat
     9001 ttttagattg tttcaatttt aacaatcctt cgtacatatt gaaagctgtt ggatagaagg
     9061 gtgcattttt aggtacttgg tcaatcatac gagtcgtctt ttctgtatct aagaataacg
     9121 aagaggcacc cccaacgaca attaagcgtg tatcggttcc ttcaagcatc tcaatcaaat
     9181 gggacaaaga agtttgatgc atttcttctt ttcctttggg agcgttgaaa gcatcaacga
     9241 caacatcaaa agatgcaaga tcagtagttg tcaactcaaa taaatccttt tgtataaacg
     9301 gaacatctga tggcaatttt tctggatgac gcacgattgc cgtcacgtct aaatctctgg
     9361 acaatgcttc gtctaaaata aatgaacctg catggccagt agctccaatg agtgcaattt
     9421 tcataaaaaa cctccttttt tattcctgtc cattgtaagc agttgctgaa tattcgtcaa
     9481 agaatccgat ttttgaaaag ctcagtgcgc attcctttca tggttcgtga tcattgggta
     9541 tagttaaatt aagaaaactg attgataaaa atagaagttg aaggagataa gaaatgaaag
     9601 agtggttaga tcgtgccttt ttaccaatga taacatctgt tgtgaatctt agacccttaa
     9661 ctgctttgcg taatggaatg acttacacga tcccaataat tattgttggc tcgctgtttt
     9721 tcttatttgc tcattttcct gtggattaca tcgctcagtg gatagacaaa acaggatttt
     9781 ctatgtattt tacacaagta taccaggcta catttgatgt gatagccttt tggatcgttt
     9841 ttgccattgc atacgcttat gcaaaagaag aagacatcga tcgcttacca gtcggcataa
     9901 cagccttgtc tgcttatcta ctgcttattc agccactaaa tactcagata gatactgtct
     9961 ggacaggtgc tgaagggata ctaggagcag ttacaactgg gctcatcatc ggatggggct
    10021 atgcacgttt agtaagactt gcgaaagggt ggaagcaaag agagaagata cctgaaaatg
    10081 tttggcagtc ttttacttca ttgattccaa tcattgttat agtcactttt acgcttatcg
    10141 gttcagcggt ttttcaacag ttgttcagaa tgactgttgt tcaaggagta tggacatatg
    10201 tccagcttcc cattcaatcg ttcatagaca cgttgtttgg tgtgctttta ttaggttttt
    10261 ttgttccgtt tttatggtta tttggtcttc atggatctgc gatggtcaat gggttggtta
    10321 gtcccgtctt gcaagcgaat tcattagcga atgcagaaat cttagcctca ggacaagagt
    10381 tgacggtggc aaatggcgga catatcgtta ctcagcaatt tctcgatcag ttcatgacgg
    10441 ttacgggtgc tggattgacg ttaggggctg tattttttat gacggttttt gcgaaatcga
    10501 agaaatacag agaattagga aatcttgctc ttttaccagc ctttttcaac atcaatgaat
    10561 caattatttt ttccacaccg atcgtgatga acccgatgat ggctgttcct tttattttcg
    10621 caccggtcgt ttcaggattg attacctatt ccgctttata ttttggattc gttcctttat
    10681 ttacagcagt ccaagtccct tggacaacac cgccgatcct ctcgggtttc ttagtaggcg
    10741 gagtgcctgc agcgatttta caaggtatcg tgttgtcaat tagttttttc atttatttcc
    10801 catttttgaa aaaaatagat tctgcaaact gcaaaaagga aagacagaca aaaaatgatg
    10861 gagcagcaga aaagatgaac gactaaaaaa caaaaatact cctgtcgctt tagattggga
    10921 aaatcaccaa tctaggatga caggagtatt tatagtcatg tttatactag ccctttttgg
    10981 acagctaatt gaacaagaac aactaatgtg ttcattccta cgtgtgtgat catcgatgtc
    11041 cagatccgac cagttgcttt gtactctagg ctgaagaaga aacctaggaa aaagtagatt
    11101 agcagatgac catcattatg agcaaacgca aacagcaaag agctgatcac tgctccgacc
    11161 caaaaatttg aatatcgact gatgatcccc agtatactgc ggcggaatac gaactcttcc
    11221 atgatgggtc cagcaacagt tgtcgccaaa ataaaagcgg gtgcttccat gatcatctgg
    11281 atgatatttt gtgtattctc ggaaggggtt gctttgccaa acaatacaga ttctaacatg
    11341 acagcgatgc tttgcagaat caatgcgaca aagattccaa ccaatccata aataacaatc
    11401 tttggagcag aagctttttt taccgctgat tcgatcgcta agggttcacg ttgcttaaaa
    11461 taaaaaatag ctaggacaac ggcgcctagt atatacatga ctgcagttgc tgtgatcaat
    11521 tcagacgtag attttacaag tcctatcgaa gctaggatca agggtgagat aaaaattaga
    11581 ccatagcaaa aaatgctgag gaagctatat ttttttaaag acatgtaatt cctcctttta
    11641 agggataaca agcatgcaag gcaagcttat atcgattgat ttcaattata tcataatttc
    11701 aataagcagc tgtcagaata aaatttcttt tctttgattt aaaagcttgc aaaaaagagg
    11761 gagaatgagt attattaaaa atgtaggtta gcactcgaag gttaagagtg ctaacagatt
    11821 aaaaaaagag ttatcggagg gatttatcgt gttaaaacca ttaggtgatc gcgtcattat
    11881 cgaagtcgca aaagaagaag aaaaaactgt cggaggaatc gttttagctt ctgctgctaa
    11941 agagaaaccc caaactggga cagttgttgc agtcggtgaa ggccgtttgt tagaaaacgg
    12001 tgaaaaagtt cctgccgctg taaaagcagg cgaccaagtt atgttcgaaa aatacgccgg
    12061 tacagaagta aaatacgaag gaaaagaata tctgatcgtc gcaggaaaag atatcatggc
    12121 gattgttgag taaatttgga agataaaaaa ataatcaaaa ataatcaatg aggtgaatca
    12181 atcatggcaa aagaattgaa atttgcagaa gacgcacgcg cagctatgtt acgcggggta
    12241 gataaattag cagatacagt gaaagtaaca ttaggaccta aaggccgaaa tgtcgtacta
    12301 gaaaaatctt acggctctcc attgatcaca aatgatggtg tgacgatcgc aaaagaaatc
    12361 gaattagaag atcattttga aaacatgggt gcaaaacttg tatcagaagt agcatcaaaa
    12421 acaaatgata ttgcaggaga cggtacaacg acagcaactg ttctgacaca agcaatcgtt
    12481 cgcgaaggtt taaaaaacgt aactgcaggt gctaatcctc taggcattcg tcgcgggatc
    12541 gaattggcaa caaaagcagc agtcgaagaa ttgcataata tctcaactgt tgttgattca
    12601 aaagaagcca ttgcacaagt agcagctgtt tcttcgggtt ctgataaagt tggacatttg
    12661 attgctgatg caatggaaaa agttggaaac gacggtgtca tcacaatcga agaatcaaaa
    12721 gggatcgaaa cagaactaga tgttgtagaa ggtatgcagt ttgatcgtgg ttacttatca
    12781 caatatatgg taacagataa cgacaaaatg gaagctgttc tagaaaaccc atatatcttg
    12841 atcacagaca aaaaaatctc taacatccaa gatattctgc cattgttaga acaaatcttg
    12901 caacaatcac gtccattgtt gattattgct gacgatgtag atggtgaagc attgccaact
    12961 cttgtattga acaaaatccg tggaacattc aacgtagtag ctgtaaaagc tcctggtttc
    13021 ggcgatcgtc gtaaagcaat gcttgaagat atcgctattt tgacaggtgg tacagtaatt
    13081 actgacgatc taggtttaga attgaaagat gtaacaatcg aaaacttagg aaatgcttct
    13141 aaagttgttg tagataaaga caatacaacg atcgtagaag gttctggaga aaaagaagca
    13201 atcgaagctc gtgtgcaatt gatcaaaaac caaattgctg aaacaacttc tgattttgac
    13261 cgcgaaaaat tacaagaacg cttagctaaa ttagcaggtg gtgtagctgt tgttaaagtt
    13321 ggtgcagcta ctgaaacaga attgaaagaa ttaaaattac gcattgaaga tgcattgaat
    13381 gctacacgtg ccgcagtaga agaaggaatg gtctcaggtg gtggtacagc tctagtgaac
    13441 gttatcagca aagtttctgc tgtagaagca gaaggagacg tggcaacagg tatcaagatc
    13501 gttgttcgtg ctttagaaga accaattcgt caaatcgctg aaaacgctgg ttatgaagga
    13561 tcagtgattg ttgataaatt gaagaacgtg gaactaggca ctggtttcaa tgctgctact
    13621 ggagaatggg taaacatggt tgaagcaggt atcgtagacc caactaaagt aacacgttct
    13681 gccttgcaaa atgccgcttc cgtttctgcc ttgttattaa cgacagaagc agtcgttgca
    13741 gataaaccag aaccagcagc gccagcagct cctgcaatgg atccatcaat gatgggcggt
    13801 atgatgtaag aaaaagcaac ttgcttatct acaactgatc attagtgaaa gacagataaa
    13861 aagaggctgg aacagaagcg tttaactcca agaaataaga agaaattcac ggaaattgct
    13921 tttcaaattt ttgtgaattt cagcttattt ccgaaggagt tgcttctgct tccgctgttt
    13981 atacgtgttt agagcgtgag acaaaagtgt tctttacttt tgtcccacgc tcttttttgt
    14041 gtgtaattat aatgaaaata gttattttta atagttataa tattttatct attaaaagta
    14101 aatatccatt c
//
LOCUS       DACDEG010000065        13748 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.067, whole genome shotgun sequence.
ACCESSION   DACDEG010000065 DACDEG010000000
VERSION     DACDEG010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.067"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..864
                     /locus_tag="IV142_001929"
     CDS             <1..864
                     /locus_tag="IV142_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8485584.1"
                     /translation="PMSQMMDFLSIFTDGMVAGSRILKIMDTEEYAPQQNPGADQMIT
                     SGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGSGKSSIINVLMRFYEFYE
                     GQILIDDHDIREYPMEELRKKMGMVLQDAFLFYGDIAGNIRMMNPAITDEQIRHAAEF
                     VQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVTDPKILVLDEATANIDTE
                     TEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGHIVEHGNHETLLAQNGLY
                     ADMYKLQSSGI"
     gene            1190..2041
                     /locus_tag="IV142_001930"
     CDS             1190..2041
                     /locus_tag="IV142_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8485585.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEDLQVHLNDSVYIALTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSIHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFSCIEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            2138..4018
                     /locus_tag="IV142_001931"
     CDS             2138..4018
                     /locus_tag="IV142_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8485586.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEAEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVPFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTETAIYGITLPKK
                     KPFIYSMIGGAVSGIYLMASGTISYTMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(4058..4960)
                     /locus_tag="IV142_001932"
     CDS             complement(4058..4960)
                     /locus_tag="IV142_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8485587.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            5191..6594
                     /locus_tag="IV142_001933"
     CDS             5191..6594
                     /locus_tag="IV142_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8485588.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            6594..8027
                     /gene="ascB"
                     /locus_tag="IV142_001934"
     CDS             6594..8027
                     /gene="ascB"
                     /locus_tag="IV142_001934"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8485589.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQVKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            8172..9098
                     /gene="trxB"
                     /locus_tag="IV142_001935"
     CDS             8172..9098
                     /gene="trxB"
                     /locus_tag="IV142_001935"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP8485590.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            9312..11039
                     /locus_tag="IV142_001936"
     CDS             9312..11039
                     /locus_tag="IV142_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP8485591.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEEAKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            11111..11404
                     /locus_tag="IV142_001937"
     CDS             11111..11404
                     /locus_tag="IV142_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP8485592.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(11496..12356)
                     /locus_tag="IV142_001938"
     CDS             complement(11496..12356)
                     /locus_tag="IV142_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP8485593.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLKQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            12448..12876
                     /locus_tag="IV142_001939"
     CDS             12448..12876
                     /locus_tag="IV142_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8485594.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            13038..13214
                     /locus_tag="IV142_001940"
     CDS             13038..13214
                     /locus_tag="IV142_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP8485595.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            13241..13687
                     /locus_tag="IV142_001941"
     CDS             13241..13687
                     /locus_tag="IV142_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP8485596.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         4498 a         2350 c         3074 g         3826 t
ORIGIN      
        1 ccaatgtctc aaatgatgga ctttttgagc atcttcacag atgggatggt tgctggtagt
       61 cgtatcttga agattatgga tacagaagaa tatgcaccac aacaaaaccc tggagcagat
      121 caaatgatta cttcaggtaa gattgagttt cgcaatgtaa gcttttctta tgatggagag
      181 cacaatgtat taaacaatat ctcttttgtt gctaatccgg gagagaccgt tgcattagtg
      241 ggacacaccg gaagtgggaa aagctcgatc atcaatgtgt tgatgcggtt ttacgaattt
      301 tatgaaggtc agatattgat tgatgatcat gacattcgag aatatccaat ggaagagtta
      361 cgtaagaaaa tggggatggt cttgcaagat gctttcttgt tttatgggga tatcgcagga
      421 aatattcgga tgatgaatcc agctatcaca gacgaacaga tacgccatgc ggctgagttt
      481 gttcaagcaa acaaattcat cgatgaatta ccaaaaggat atcactcaaa agtgatcgaa
      541 catggtgcca gctattctag tggacaacga cagctgatca cttttgcgcg aacaatcgtg
      601 actgatccta aaattctagt attggatgaa gcaacagcaa atatcgatac tgaaacagaa
      661 ggattgatcc aagaaggatt ggcgaaaatg cgacaaggac ggacaacgat tgcgattgcc
      721 catcggctgt caacgattcg agatgcagaa ttgattttag ttttagacaa aggtcatata
      781 gtagaacacg ggaatcatga aacacttcta gcgcaaaatg gtttgtatgc agatatgtac
      841 aaactgcaaa gcagtggtat ataaaagatg ttgtgacagt agcattttga cctaagcact
      901 agagtgaaaa taaaacatag cttcccatat ttttcatctg tgagagttag tgtcgtagtt
      961 accagattta aaaaagacga cgtcagctag tcagaagtgc ctaatagccc ctaagaaagc
     1021 gttgacaaaa tcgtttgctt tcgataatac aaagaaaacg atacaacttg gatagtgatt
     1081 gcctaatgca agctaaacct gatgagtctc ccatggtttc atggacggct ttttgggttt
     1141 ttctttttgt tttggataca gccctatata tcatggaaag gagcaaaaca tgctgattga
     1201 gaaagtactg aataacaacg tcgtaataac cagagatgaa agaaatcagg aaatgatcgt
     1261 gatgggaaaa ggtttggcat tcaaaaagaa agtgggtgac ccgatttttg actcatgcat
     1321 cgacaaaaaa tttcagttga caaatgacgg actttcacaa cagttccaag aattggcaca
     1381 agcgattcct cttgagtact tagaaatttc ttgtgaaatc attgaatata caaaagagga
     1441 tctacaagtc catttgaatg attctgtgta tattgcattg acggatcatt tgtatacagc
     1501 tatcgaacgt caaaaaatgg ggatctttgt gccaaatgta ttgctgtatg atatcaaaca
     1561 actgtttcct aaagagtatg ccattgggaa aaaaacagtg aagaaaatct cggataaata
     1621 ccacatcaag ttatcagaag acgaagccgg atttatcgca ttgcatttag tcaatgcaca
     1681 aggcattcaa agccagcaag acaataccta cctgtttacc acaacaatac aggatatttt
     1741 aaatatcgtc cgctattact ataagattac atttgatgaa gattcaatcc atttttttcg
     1801 attcgtcaca cacctacgtt tttttctgac acgcgtccta gatggagaga aatcagaagt
     1861 gggagaagtt tctgaggaat tgttccaggt tattcaaaaa aaatataagg acacgtttag
     1921 ttgcattgag aaaatagcag actttttaac cggtgctttc gactaccaga tgtccacgga
     1981 tgaaaaaatg tatttaacga tccatactgc gcgtttagtg catgagaatc gttgtaaata
     2041 aataaaaaag gaggacaaat taacaagaac gtatgaaaat aagggtagtg acattcagtt
     2101 ggctaaacct tccactaatt aattggaggg aatagaaatg ggaaaatacc aggaattagc
     2161 agaaaacatc gtaaagaata tcggaggaaa agaaaatatc cgtagcttga cacactgtgt
     2221 gacgcgactt cgtttcaaac taaaggagga aaataaagca aaggatgatg ttttgaaaaa
     2281 gatggaaggg atagtcacca tcatgaaaag cggcgggcaa tatcaagttg taatcgggaa
     2341 tcatgtgcca gatgtctatg cagaagtact gacagtagcc gggatttccg atgaagcaga
     2401 agaagaggtt tcttcaagta gtaatctgtt caatcgatta atcgacatat taagtggctg
     2461 tttccagcct tttttaggtg ctttggctgc agcgggaatg gtcaaaggat taaatgcttt
     2521 gttcttgttt ctcaaatttt atactgaagg atcgggtacg tacatcatgc tgaatgggat
     2581 aggggatgca attttttatt tcatgccagt gatcttaggg tacacagcag cgaaaaaatt
     2641 tcatctgaat ccgatggtgg ggctcgtgat tggtgcagca ctttgttatc cgactgtaca
     2701 aggagcagca ctacaaagtg gtttccctga tggcagtcct gcgccttata gcttgctggg
     2761 tcttccggct tatgaaacat ttttgggtat cccatgggtg ggcagcagtt ataccagtag
     2821 cgtcgtacct gttatcttca ttatcgcatt tgccggacaa gtccaaaaaa ttgccaagaa
     2881 atggattcct gcagtcgtcc agaccttttt ggttcctttt tctgtgttat taatcgcact
     2941 tcctgctggc tttttgatta tcggtccgat tgtcagtata ctgacagatc tattaagcac
     3001 gggattccaa gcattgatgg acttttctcc agctgtgtat ggtgcagttc tagggtttct
     3061 ttggcaagta ctggtgattt tcggacttca ttggagtatt atccctcttg cgattatgca
     3121 gctagcgcaa aatgggtgga gtcagatgtt agtaggcgtt tatggcgcga gttttgcaca
     3181 gacagcagtg gtattggcga tgtatttcaa gttcaaggat caaaaaatga aggcgctttg
     3241 catgccagct gtgatttcag gaatcttcgg cgtaacagaa acggctattt atggaatcac
     3301 cttaccaaag aagaagccat ttatttactc gatgattggt ggtgctgttt caggaatcta
     3361 tttgatggca tctggaacaa tcagctacac aatgggcggg ttagggattt ttggggtggt
     3421 aaactacatc acggaaacag acgccagtgg tatgtttcaa gcgtttatcg gcattgctat
     3481 tgcttcggta atcgggtttt cgctgacttt tttcttttgg aaggatgaag caggtgaaac
     3541 agatgatata cagaaaaaat caggtaaaga aattgtaaaa acaccgatac aaggacaaat
     3601 cgctccacta aatgcagcaa aggatgcagc ttttgcacaa ggaacactag gtcgaggaat
     3661 cttgatctac cctgaaaaag gagaagtacg tgcccctttt gacggaacag tcatgaccct
     3721 gttcccgacg aaacatgcaa ttggaatggt ttctgaaaca gggctggaat tgctgattca
     3781 tgtcggattg gatactgtgc agttggaagg gaaatatttt gaaagcttag tgcagcaagg
     3841 agaaagagtg aaacaaggcg atctgttgct acgcttcgac aaaaagaaaa tcgaaaagga
     3901 aggatacagt ttagaaacac ctgtgatcgt caccaattac acggattatt tggatatttt
     3961 ggaaaaccaa ggggagacgg tcgggccaga cgatgcactt atcaccgcac tgacctgaac
     4021 ggttaggtga agaatggact aggcttaact attcttttta ttccacttct ttattttcaa
     4081 aattgaaagg ctctttcatg atggctgagc tgatagggcg tttatcgaat ttttgaccga
     4141 cagaaattag atggactaaa ttctgttgat aattttctgc aaaaacacaa ctatataaca
     4201 cgtctagaag ataacaaatt gatgtattga tcgtaaaatt accaattttt gaatacagtc
     4261 gctcgcgtgt cgtaagtcgc aaagcggctg tactcattgc agatagcgtt gaatcaccga
     4321 tactggtcaa agctaaaatt ggaacatcat tttgtttcaa tatttctgcg gtacgaagga
     4381 tcacattgtt ttcacccgta taagaaatca gaatcccgca catatcactc gtgcaattgt
     4441 acgcttcata gaacgattca ccaaaagtgg tgacaatcgt gacatccatt ttgatgcgct
     4501 tcattttcag catgaaatct tgagaaatca aagtattcga gttactggcg aaaatcacga
     4561 ttttttttgc ttgaagcatt agttgtttgg ctttttgcag ttggtcatga tgaatgagag
     4621 aaagtgtatc atgaatcgtc atctgttcta aagaagccat tttatttgcg atcgtcataa
     4681 taccgtctgt ggtggaaaaa ggaagattcg catctacgtc ttgaaaattc ccttcaagat
     4741 aggctgtttc agctaaaaaa gcattacgaa aactgaccca gccatcaaaa cccatttttt
     4801 ttgctacacg gatgagtgta gacgggtgga catagcatcg ctcagcgatc tcttggatgg
     4861 tcatttcgcc aagtgctgga gaatcgctta agagaaactg gatcacttca ttttctgaag
     4921 gagaaaagga gatttctttg attttttcac gtaataacat gtagttacct tcttcttatt
     4981 tttttacgta aattataact tttttgaaaa tgttttgtaa acggatttat actatataac
     5041 ttaaaataga ctttttttga caagatattt acaaaaatcc actagaaaac agtaactttt
     5101 gtaaatgtta ctggaaagaa acggatttct attgtttgtt ttgtttacaa aaaagataat
     5161 gaaagtgtaa aaaagtaaag gagtgagaac atggcaaaag gaatcaaaat cgctacgatt
     5221 ggtggaggca gcagttacac ccctgagttg atggaaggat ttatcaaaag atatgaggaa
     5281 ttaccgatac gagaaatctg gctcgtagat gtagaagaag gaaaagaaaa gcttgagatc
     5341 gttagtcaga tgtctcagcg tatgtgggat gcttctccgt atgaagtcaa aatctatgca
     5401 actctggatc gggaagaagc attgaaagat gcggattttg ttacgactca gtttcgtgtc
     5461 ggtcaattag atgcacgaat caaagacgag cggatcccta gttattatgg gatgattggg
     5521 caagaaacaa atggtgctgg cgggatattc aaagcttttc gtaccgttcc agtggtcttg
     5581 gcaatcgttg aagacatgaa gcgtttatgt ccaaatgctt ggctgatcaa tttcgcgaac
     5641 ccaagtggga tggtgacaga agcagttatc agatatggca aatgggataa agtgattgga
     5701 ttatgcaatg tgccagtaat ggcgatgatg acagaaccag caatgcttgg caagaaaaaa
     5761 gaagagctga tctacaagtt tgccggtcta aatcatttcc attggcacaa agtagcagat
     5821 aataaaggaa atgaccgcac ggcagaattg attgatcgtt tatacgatga agacaatgga
     5881 ttgccgaaaa atatctttga tatcccgttt ttcaaagaac aattaaaaca aatgaatatg
     5941 atcccttgtg gctaccaccg ctattactac cgtgaagaag aaatgctgga acatgcttta
     6001 gaagaatatc aaacgattgg tacaagagcg cagcaagtga aagaaacaga agcagagcta
     6061 tttgaattat acaaagatcc agaattagac cataaacctg aacaattgca aaaacgaggt
     6121 ggcgcttatt attcagacgc tgcctgtgaa acgattgctt caatctatgg aaataaagaa
     6181 acacagatcg tggtatctac aaagaatgat ggagctgttc ctgacctgcc ggcagattgt
     6241 gtagtagaag tcactgccta tgttggcgca caaggagcga gaaatgtagc gtttggttct
     6301 cttcctgctg ctgaaagagg ctggttgcaa gtaatgaaga atatggaatt actgacgatc
     6361 gaagcagctg tgactggtga ttatgggaca gctttacagg cgtttacgat caatccgctt
     6421 atacgatcag gaaagacagc aaaacggatc atggatgagt tgtttattgc acaccagcag
     6481 tatttaccgc aattcaagga aacaatcagc cgattaaaag aagaaggaat cgaagttcaa
     6541 gatgaactgg caagagaatt atcaggtaat acacatataa tggaggaagt ataatgggat
     6601 tcagaaaaga ttttttatgg ggcggtgcta cagcagccaa tcaatgtgag ggcggttatg
     6661 atgaaggtgg acgcggacta gcaaacgtag atgttgtgcc aatcggaaaa gaccgttttc
     6721 ctgttatcac cggaaagaaa aaaatgtttg aatttgatga ggatcatttt tatccagcaa
     6781 aaattgcaat cgatatgtat catcgttata aagaagatat tgctttgttt gcagaaatgg
     6841 ggttcaagac ttatcgaatg tccatcgcct ggagtcgtat ttttccaatg ggggatgaaa
     6901 aagagccgaa tgaagctggc ttgaaatttt atgaggatgt ctttaaagaa tgccaaaagc
     6961 acggaatcga gccacttgtg acgattacgc attttgattg tccaatgcat ttgatcaaag
     7021 aatacggtgc gtggcgttcc agagaactcg ttggttttta tgaaaatctg tgtcgagtca
     7081 tcttcaatcg atataaagga cttgtcaaat actggctgac attcaatgag atcaatatga
     7141 tccttcatgc accattcatg ggagcaggac tgtactttga agaaggagaa aacgaagaac
     7201 aagtcaaata tcaagcggca catcatgagt tggttgcaag tgccatcgca acgaagattg
     7261 ctcatgaagt agatccggag aacaaagtag gctgtatgct ggcagcagga gcatattatc
     7321 catacacttg caatcctgaa gatgtttggg cttctagaaa atcagatcga gaaaattatt
     7381 tcttcattga tgttcagtca cgaggagaat acccagctta tgcattgaaa gaaatggaac
     7441 gaaatggcat tcagatcgaa atggaaccag gggatgccgc attgctgaaa gaacatactg
     7501 ttgattttat ctctttttct tattattctt cccgagtttc gactaccgac aacgaactct
     7561 tggaacagac tgcaggaaat atctttgctt ctgtcaaaaa tccatatctg caagcaagtg
     7621 aatgggggtg gcaaattgat ccattaggct tgagaataac gatgaatgat atttatgatc
     7681 gttatcaaaa accattattt attgtagaaa acggcttagg tgcagtcgat acgccagatg
     7741 agaatggtta tgtggaagat gattaccgaa tcgattacct ggcagcgcat atccaggcga
     7801 tgaaggatgc agtagaacta gacggagtcg atctattagg ctatacgact tggggatgta
     7861 ttgatttagt atcagctggt accggtgaga tgaagaaaag atacggattc atttatgtcg
     7921 atcgtgataa ccaaggaaat ggcacattag cccgttcaaa gaaaaaatca ttcaactggt
     7981 ataaaaaagt aattgattca aatggagaaa atctttcgaa cgattgatca atgatttata
     8041 aaagtctatt gtttttcagc ggatttttcg agtataataa agctattcga gaaacgcaag
     8101 cagaacaata gacttttttt cgcattggct gtaaagtatg cttacttgat taagtaaagg
     8161 agaatctaca tatgtatgat gtaatcgtta ttggcgcagg tcctgctggg atgactgctg
     8221 ctttatatgc ttcacgttca aacttatccg tattgatgat cgaacaaggt gcccctggcg
     8281 gccaaatgaa caatacggca gaaatcgaaa attatcctgg attcgattcg atcatgggcc
     8341 ccgatctagc ttataagatg tatgaaagtg tctcaagatt tgggacagaa aatgcttatg
     8401 gaatcgttca agatatcaag gatcacggca gccataaaga agtcatctgt gaagataaaa
     8461 gttacgaagg aaaaacagtc attatcgcaa caggctgtat ccaccgtaaa ttaggcgtac
     8521 ctggcgagga agaatttgct ggacgaggcg tttcttactg tgcagtctgt gatggtgcgt
     8581 ttttccgtaa caaacgtcta gtagtcatcg ggggtgggga ctcagcagta gaagaagcca
     8641 tctatttgac acaatttgct tctgaagtag tgatcgtcca ccgtagagat gaattacgtg
     8701 cacaaaagat catccaagac cgtgcatttg caaatgaaaa aatttccttt atgtgggata
     8761 cagtcgttga cgaaatcgtt ggaaacgaaa tggtagtgac aggtgtgaaa gcacgtaatg
     8821 tcaaaaccgg caatgaattt gatttagcag cagacggagt attcatctat gttggactgg
     8881 atccattaac tgcacctttt agagaagcag gtttgacgaa cgaagctggc tggattccaa
     8941 cagacaacga aatgaaaacg aatatgccag gcgtttttgc catcggagat gttcgtgaaa
     9001 aagatttgcg acaaatcact acagctgttg gtgacggcgg tattgctgga caacaagtgt
     9061 ataaatactt ggaatcttta gaagaaacaa ctgcataagt ttaaaaagag tagaagaacg
     9121 ggttcaattg ttcttttact cttttttttc gcgtgaaatt tactgaatct gatttatgcg
     9181 aacacgtcca acaccttttg ataaaagaat cctttcttga tttaagcgtt ttttggtcct
     9241 catactttca tatctaataa gtgaatgcta tacttattaa gaaccatatt tcagaaagag
     9301 gtgttatttt catgtcttgg gaacaagtgt atgaacaatg ggtacatgaa gaaaatttag
     9361 aagaaaactt aaaaaaacaa ttaaatgatc tgggtgaaga tcctgaaaaa cttgaagatg
     9421 ctttttatgc accgttggaa ttcggtactg caggcatgcg gggaatctta ggacctggga
     9481 tcaatcgaat gaacatctat acggtaagac aagcaacaga aggactagca cgtttcatgg
     9541 atacacaagg tgaagaagca aaacgccgag gtgtcgctat tgcttatgac tcacgccatc
     9601 agtcaccaga atttgcaatg gaagcagcca aaacactggc acatcacaat atcccgtctt
     9661 atgtattcga aagtctgcgt ccaacaccag agctttcttt tgcagttcgt catttgaaga
     9721 cgtttgcggg aatcatggta actgcatctc acaacccagc ggcttacaat ggatataaag
     9781 tctacggaga agatggcgga caaatgcctc ctgcagacgc agatgcattg acgaaatatg
     9841 tccgtgaagt cagcaatccg ctaaaagtcg aagtattatc tgacgaagaa gctaaacata
     9901 gcgggttgat cacgatcatc ggagaagaag tggacgctgc ttatctagaa gaaatcaaag
     9961 cagtgacgat tgatcgtgaa ttggtagaaa caatggggaa agatttgaaa cttgtgtaca
    10021 cgccacttca tggaacaggg aaaatgttgg gcgaacgtgc gttgaaacaa gctggattcg
    10081 aacaattcgt actagttccc gaacaagctg tggcagatcc agatttcact acggtcaaat
    10141 cgccaaatcc agaagaacat tcagcttttg aatatgctat acgacttgga gaaaaagaag
    10201 gagctgactt gctgattgca acagatcctg atgcagaccg tttaggcgca gcggttcgtt
    10261 tgccagatgg ttcttatgaa gtattgactg ggaaccaaat cggggcattg tttgctcaat
    10321 atattttgga agcacatcaa tcaagaggca ctttaccaga aaacgcagcg gtattgaaat
    10381 caatcgtttc cagcgaattg cctacagcaa ttgcggaaca ctacggtgct accatgttca
    10441 atgtgttgac agggtttaaa ttcattgctg aaaaaatcca acaattcgaa gaagaccatt
    10501 cacatacttt tatgtttgga ttcgaagaaa gttatggtta tttagttaaa ccttttgttc
    10561 gcgataaaga cgcgatccaa gcattgcttt tattagcaga agtagcagct tactataaaa
    10621 aacaaggcaa aacgttgtat gacggcttgc aaacgatttt tgaaacctat ggctattttg
    10681 cagaaaaaac gatttctgtg acaatgagcg gacaagaagg ttctgcgaaa atctctgctt
    10741 tgatggaaaa attccgtaaa gaagcaccga cagaatttgc cggagcaaaa gttattcaaa
    10801 cagaagactt taaagaattg acgagaacag accgtgaagg aaacgtagaa aaactaacaa
    10861 caccgccatc cgacgtattg aaatatacat tggaagaaaa tggctggatt gctatccgcc
    10921 cttcaggaac tgagccgaaa atcaagtttt atattggtgt aaaaggaagc tctacagaag
    10981 acgcagccga aaaagtagcc tcttatgaag cgattatcaa aaagatgaca gaagaataaa
    11041 aaacttgaga ctgatggaaa aagtggtaaa ctttagaagt cagtcttttt tcaagttgga
    11101 ggtgaatgga ttgccagaca cgcaaaaatc aaaagtagag aaagtcagtc aattattcaa
    11161 aatgttgagt gatcccactc gtcttaaaat tctgctatat ctgaaagacg gggaacaaaa
    11221 tgtgacagca atcacccaag cagtggaaat ggagcagtct gctgtatcgc atcaactacg
    11281 tttattaaga gaaaatcatg tcgtcaagtc tcatcgcgag ggaaaagcga ttttgtatag
    11341 tcttgatgat tctcatgtac tggatatttt gaaccagaca ctcaaacatg tggaacatca
    11401 ataacgatag gtgcaaatag ataaacactg gagtgtgaca acgaaatttc ttcgttgtca
    11461 cactccagtg ttttttaaga aaaagacaaa aatgattaat cgcttttttc tgttgtttca
    11521 gttccataat aactatgatc ttctaggtct aaagcattca aaataagcga agccgtttct
    11581 tcaatagata aagatgccac attgatcact acacatccta gtttttcata aagatcattc
    11641 gcaaaatcga gttcagctct aattttttcg atatcggaat aagcagtatc agggtttaat
    11701 ccgtaagagc gcatccgttc tttacggata ttatttaaaa tatctgggtt attcgttagt
    11761 cccacgattt ttttaggatc gatctcccaa agctgtttgg gaatatgtgc ttgtgggatc
    11821 aaaggtaaat tggcaacttt taaatttttg ttagctaaaa acaagctcaa aggcgttttg
    11881 gaagttcggg atacgcctag caaaacgaca tctgcttcta aaaaaccacg aggatctttc
    11941 ccatcatcgt attttacagc aaattccatt gctttgatcc gtttgaaata gttctcattc
    12001 aataaatgga gggcaccagg ttgccgaatt ggcttgatac cggttaaacg ttcaatttct
    12061 gatatcggtg gcgtcatcac atcaaaacta aataattgat gttcttcaca aaaagaacgt
    12121 gttatctcga tcagttcttg attgattaat gtatgcagga caagtgcttt ttcttctaat
    12181 gcatcttgca atgcctgctt taatgttttt tcatctgtca cgaacgttct gcggaatagg
    12241 ttgaactcaa cggtaggata ctgagccata gtagcttgcg ctagttttgt cgcagtttct
    12301 ccagcagagt cggagatgac aaaaaaagta acgatttctg tagaatcgga agacatggat
    12361 aaaacctcac ttgcattttt tttatgttta ttataccata ctctagaatg cgtacttcgt
    12421 aatcattttg atttggagga ataagtcatg aaaaaacaga cattgatcga acaatcatta
    12481 gaaacattga aaaataacgg ttttaaatat actaaaaaaa gagaagcatt gttaacttat
    12541 ttagtgaagc gtaaccgcta cgtatctgct aaagaagttt ttgactatat gaatcaggaa
    12601 ttcaaagggg taagttatga tacgatttat cggaatctcc atgattttgc agagatcgat
    12661 ttactggaag aaaccgattt aaatggagaa atgaagtttc gttttcattg ctgtcagggg
    12721 gaaatcggcc atcaccatca ttttatctgt acagtgtgcg gaacaacgaa agaaatccat
    12781 atgtgtccga tgaattattt cgaagaacag ctaggtggat gcacgatcga agggcataga
    12841 ttcgagattt tcggaagatg tgaaaattgt caataagggt ttaaataaaa taacatttga
    12901 taattttaaa aaaaagcaag atttccttga cggtcaaaac agcattagat ataatattta
    12961 caggacagtc ttgttttttg agatatattt caataacaat tacggttact ttttaagctc
    13021 ggagggaggg aattaacatg tcaaaaacag tcgttcgcaa aaacgaatca cttgacgatg
    13081 ctcttcgtcg cttcaaacgt tccgtgtcaa aagcgggtac tttacaagaa tctcgtaaac
    13141 gtgaattcta cgaaaaacca agtgtaagac gcaagaaaaa atctgaagca gctagaaaac
    13201 gcaaaaaatt ctagttctgt gatgaattgg agttggtagt gtgtcactac taagtagatt
    13261 aaacgatgac atgaaagcag cgatgaaagc gaaagataag gaatctttac aagtcatccg
    13321 aatgatcaag tcatctatac aaaacgagca gattaaagaa ggtcacgact tgacagaaga
    13381 agaagaacta acggtgttgt ctcgcgaaat gaaacagcga cgtgattctt tgcatgagtt
    13441 tgaagaagca ggacgtgacg atttagctga aaaagtaaaa agtgaaattg taatagtcga
    13501 aaaatatatg ccggaacaac tttccgatga agaaattcga caacttgttc aagaagcaat
    13561 cactcaaact ggtgcttcat caatgaaaga atttggcaaa gttatgggtg caattatgcc
    13621 taaagtcaaa ggaaaagcag atggaaacca agtgaatgcg attgtgaaag aactattaca
    13681 atcttaattt tatcatttaa actgcggaca gtgattgtcc gcagtttttt gtgttcgtaa
    13741 aaaaagaa
//
LOCUS       DACDEG010000066        13670 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.068, whole genome shotgun sequence.
ACCESSION   DACDEG010000066 DACDEG010000000
VERSION     DACDEG010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.068"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..322)
                     /locus_tag="IV142_001942"
     CDS             complement(<1..322)
                     /locus_tag="IV142_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP8485597.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKW"
     gene            complement(469..1242)
                     /locus_tag="IV142_001943"
     CDS             complement(469..1242)
                     /locus_tag="IV142_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8485598.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(1416..2276)
                     /locus_tag="IV142_001944"
     CDS             complement(1416..2276)
                     /locus_tag="IV142_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP8485599.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(2276..3694)
                     /locus_tag="IV142_001945"
     CDS             complement(2276..3694)
                     /locus_tag="IV142_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8485600.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(3751..4200)
                     /locus_tag="IV142_001946"
     CDS             complement(3751..4200)
                     /locus_tag="IV142_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8485601.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(4645..4908)
                     /locus_tag="IV142_001947"
     CDS             complement(4645..4908)
                     /locus_tag="IV142_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP8485602.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(5007..6302)
                     /locus_tag="IV142_001948"
     CDS             complement(5007..6302)
                     /locus_tag="IV142_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP8485603.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(6299..7570)
                     /locus_tag="IV142_001949"
     CDS             complement(6299..7570)
                     /locus_tag="IV142_001949"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP8485604.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(7735..8604)
                     /locus_tag="IV142_001950"
     CDS             complement(7735..8604)
                     /locus_tag="IV142_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP8485605.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(8896..10506)
                     /locus_tag="IV142_001951"
     CDS             complement(8896..10506)
                     /locus_tag="IV142_001951"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP8485606.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(11699..13039)
                     /gene="glnA"
                     /locus_tag="IV142_001952"
     CDS             complement(11699..13039)
                     /gene="glnA"
                     /locus_tag="IV142_001952"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP8485607.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(13067..13459)
                     /locus_tag="IV142_001953"
     CDS             complement(13067..13459)
                     /locus_tag="IV142_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8485608.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         3863 a         2995 c         2326 g         4486 t
ORIGIN      
        1 accacttgtg gaagaaccat tctgccacac cgatcaatag agcaggaatc aatactttca
       61 tccaaatatc gactgtatca ttcataaaat atagatagag ccacaagata actgcagctg
      121 aaccagcatc tgcgatcgtc gctgcgatgt tccctacttt acgtaagatc aacagatccc
      181 ctagcacata gatcatgact acaataatta ccccaacaat cgttgaatcc ataaagctaa
      241 caccgtataa acctgtcagc acgacccaca gaatcgccca gatcatgatt gccttcacta
      301 ccaacgcctt cacatgtttc attttctgca ctcctttctc ctggataaaa aagtaagatt
      361 atctaacaag ataacctttt cattattata ccatcggctg agagaagcga gcaaagataa
      421 ggtcctatct aaaaatgagg aagccactta gaacattcac gtaataattt atccgtacag
      481 acattcgata tggtgttcct ttactttcgc atactgttct tttgtcagtt ctttacttgt
      541 caccagataa tcgatttcat gaaaagcaat ggcttgatgc atgaatttat gtgcaatttt
      601 ttgctgtgta actaaaacgg caagccggtc tgattgtttt ctaaaagctt ttttgaccat
      661 tgcctctcct tcatgagttt ctgttactcc ttggctgaaa tctagcccgc gacaactcat
      721 tatcgctaaa tccgcataat atgacaatgc atcgttatag gcttttgaac cgttgattgt
      781 tgctcgtttc ggaacaattg cccctcccaa tacagatact tttgccgttg tatattcact
      841 caacatagca gctgtgacga tgccattcgt aatgatctgc aaaccatcaa atcttgctag
      901 gtttctgact aagtgtaaag aggttgtact ggaatccaag aataagacca tatcatctcg
      961 aatcaaagaa gaagctgtat ctgcaatctt acgtttttct gtttcttctt ctaatttggt
     1021 gatcttcatc aggtaaggca cgtcaatttt tcgattgtca ataagcatgg cgccaccgcg
     1081 aactctacgg atcaatccac ttttttccag aaaaacaagg tcacgtttaa tcgtggcttc
     1141 gctataattt actaactcga tcaaatcggt gactcttgca aattggttgt tttttagata
     1201 attcacaatc tcctgctgtc tattctccac ttcattcaac ataaaatcac cccatttact
     1261 ttatatagta acaggaaaaa agaggttgtg acagaagtga ttggcttcga gaaataagaa
     1321 gaaatttttc gaaaaaacta cttctgaaca tcgtgtgtgg ttaagagggt atgacaagac
     1381 tattgtcata ccctcttgac cacacatttt tatttttagt ataaactagc cttgcctact
     1441 gtgttcaaaa tttccatttt atgtttgacc acttcttgta cctttttatt agctgctgga
     1501 taaattgcat tcggctcgta catcgatgga ttttcactca atatttctct caaagcattg
     1561 aaaaagacgc ttttaagatc tgagctcaaa ttcactttgg caactccgta ttttactgct
     1621 tcgctgactt ctgcgtccgg attacctgaa ccgccatgta aaacaaatgg aagatcgatt
     1681 tgttggttca attctttcag taagtcgata tttaattttg gagttctgtt tttcggatac
     1741 aagccatgcg ctgttccaat tgctacagca agtgtatcga ttcctgtttc tttcacaaaa
     1801 atcttcgctt gatcaggatc tgtataaatg attgtttcag cacctccttc agcagaacca
     1861 ttattcccaa tcgttccaag ctcagcctcc actgagacat ttaccgcatg agctaacgca
     1921 accacttttt tcgtaatctg tacattgtcg tcgaatgatt tcaaagacgc atcgatcatc
     1981 acggaagaat agccgtttcg aatcgcccgt aagacatctt ctatcgtacc gccatgatcc
     2041 aagtggataa cgatcggcag tttacttgta taagcatagg ctcttactgc cgcaacaaac
     2101 tcatcactta aataggcgat ttcatcagga tgaatttcta agattactgg tgcttctaat
     2161 ctctctgctt cttcgatgat tgctttcatc atatcatagc tgcaaatatt gaaggctggt
     2221 actgcaaatt gattctcttt agccactttc aataactctt tcattgtata taacattacg
     2281 cttccacctc atttgttaga ttttcatttt tcgtccactt gtcttgtttt ttctctttca
     2341 ctgttttttt caatagtcca agcataaagg ctgtagtgac tgtacctgat gcaattgcca
     2401 agacatacat aggtaaattg ttcataacat ttggaattag catcacccaa atccctccat
     2461 gaggtgccat tgaagtaact cctactgcca tggaaatccc tgcagcgact gctgaacctg
     2521 tcatcaaact aggtatcacg cggatcgggt cagataccgc aaacggtata gcaccttctg
     2581 taatatacga agcgcctaaa atccagcatg attgacctga agtccgttct tctttggtaa
     2641 atttatgttt gaataataaa gtggctaaag caatacctag gggaggtgtc attcctgcag
     2701 ccatacaagc agcaatcggt gcatttactc cagctgacat caaaccgatc gagaaagtcg
     2761 aagtcgattt attgatcggc ccacccatat cagctgccat catgatcccg ataagtaatc
     2821 cgaacaatag cccggatgta tttcccaagc tattcagcca atttgtcaaa gcatccaaca
     2881 cccatgtgat cggttgttca atcacaaata ccatcaaaag cccaataatt aagatcgata
     2941 aaaaggggac gacaatcagc tgataaatag cagaaaattc tttttttacg ctaattttat
     3001 ttgccagaaa atcacagata taccctgcta gcacgccacc aatcattccg ccaagaaaac
     3061 ctgaaccacc tgtagaggcc agtaatccgg caacaattcc tggggcaaaa cctgcttttc
     3121 cagcaatcga tgtagcaatc cccgcgccta acgcaggaac catcatggca aacgctgtat
     3181 ctcctccgat ccttgaaaat gcagctgcta acacattata gtctgctgag ttgggatcag
     3241 ccgctgtaat cccaaaggca aaactcagtg cgatgatgat tccgcctgcg atcacaaatg
     3301 gaagcatgta attcacacca ttcatgaaat gattatagat tgtcggcttt ttctcatttg
     3361 cagctggttc tttttcatta gacacaacga atacgccaat atctctcaat gcttcgtcta
     3421 ttactacttg cggttctttg attgctcggc ttgtagagac acgctttact tttttcccgg
     3481 caaatcggtc caattcgatt tctttatcta ctgcaagaat gattgcatct gcctctttga
     3541 tttcttgttc agataactga ttttctgttt ttacaccttg tgtttcgaat tttgcttgat
     3601 ggtttagatt tcttgctgtt acttctaatt tttctgccga catataagta tgagcaattc
     3661 cagtcgggca agcagttaca ccaacgattt tcatgattat tttcctcctt tttcttcact
     3721 atcgatactc ggacaaagac tctactattt tcattcttga ctaatctgtc tgatgatatc
     3781 tgctggcgta gtggcttctt ttattccctt tctaaaatct gcattcatca atcgtctgga
     3841 gagtgtgctg agaatctcta gatgcatatc tgctgatttt tcaggaacta cgatggcaaa
     3901 aatatattca attggcattt catctatccc gctatagtcc gttaatttgg ctgtatgaaa
     3961 aaaacacagt gctggttttg tcacatattt gctttttgca tggggaatcc caaatccatc
     4021 ttctattcct gtggagatct cctcctctcg tttgaatagt gccttaacga ctttttgaac
     4081 atttcttacc ttatttgttt ttttcaacac atcccctatt cgctcaaata atgatccttt
     4141 tgaatctatc gatggatcga aaacaatcag ttcttctgcg atcaattgat caaacctcat
     4201 aaaaaacctc tcccccacaa aaaatgttaa cgttttcttg gaactgaggt cagtatagca
     4261 tcgctagctg taccgttcaa tattatgaat gaacagagga aaaagaccca tcatccgcgt
     4321 gtataagtca ttaaaatgaa cgatctagct cacaaacaat cgcaagtcta taacaaaaca
     4381 aaaagccttc cttgaaagga aagcttagct tctaatttta ggtaaatggt tttttacttc
     4441 gaaataaccc ctcttcattt cgttaatata aggtgttttt atcaagtgga taattaaaac
     4501 tatatactag tttttcattg ttttgtgcat tatgatccta cgactttttc agcatcaaac
     4561 gaagttttaa attggcaaaa aacataaaaa aacagactat ccctatcaga tagtctgcta
     4621 aacgttgagt tatcagcgtt tttattattc cgcagcgttt gcgtctttga gaccgtattt
     4681 tttgttgaaa cggtccacac gtccgtctgc ttgtgtgaat ttttgacgtc cagtgtagaa
     4741 tggatgtgag tcagaagtta cttcgacacg gatgactggg tatgtattac cgtcttccca
     4801 ttcaactgtt tcttgtgaag tttttgttga acctgacaag aatttgaaac cagttgttga
     4861 atccatgaat actactgggt ggtaatctgg atggatgttt tctttcatga ttttttcgct
     4921 cctttgccct gctccatttg ctgaaccaga gttgatttgt cgatttacaa cattaacatc
     4981 ttaacatgtt tcagtcaagg ttgcaattat cttttcagat ttctggtcgg atttttttta
     5041 ccaaaggaga catcttgaaa ttctttgaaa aattgctgat tgttttttgt tttacgcaaa
     5101 aagcggatca gttgttccgt atattctaat gaatctccag tcatattttt tcgaagtttc
     5161 caaatttctt ctaattcttc acttggcatc aataattctt cttttcgggt acctgatttt
     5221 ttgatatcga tggctgggaa gatccgacgt tcagataatt cacgtgaaag ttgtaattct
     5281 agatttccag ttcctttgaa ttcttcatag atcacgtcat ccatacggct tcctgtatcg
     5341 actaaagcgg tcgctaaaat cgtcaaactg ccaccttctt cgatgtttct tgctgccccg
     5401 aaaaatttct taggtttata caatgcggct gggtcaatcc cgccactcaa tgtacgtccg
     5461 cttggtggaa cgactaagtt ataggcccga gcaagtctag taatactatc catcaagata
     5521 acgacatctc gtttatcttc gactaaacgc attgcgcgtt ccaatactaa ttcagaaact
     5581 cgagtatggt tttgcggctg cagatcaaat gtagaagata ccacgtcgcc tttcacgctt
     5641 cgttctagat ccgttacttc ttccggtcgt tcatcaatca gtagaacgat taattctact
     5701 tccggatagt tttcggtgat accatttgcg atttctttta atacagaagt tttccctgct
     5761 tttggcggtg cgacgatcaa tccacgctga ccaaaaccga ttggagaaaa gatatcgatc
     5821 attcgggtag atagtctttg cggtgttgtt tccagttgaa gctgtctttg cggataaaga
     5881 ggtgttaagg ctggaaaatg cggacgttct tttgcttctt ccggatcttt tccattaacg
     5941 ctttccacgt gcatcaaacc ataataacgt tccgattctt taggtggacg ggcttttcct
     6001 gccactttgt ccccatttct caaactgaat cgtctgattt gtgaggcaga aatatagata
     6061 tcttctgcac ttggtccata attgattggc cgtaaaaaac cgtaaccatc ttgaccaacg
     6121 atatccaata ttccttccat ataataaaat ccttgtttct ctgcttgtgc acgaatcaca
     6181 gctagagata attccttttt attcatctta ctgtaataag gaattttaaa ttctttggca
     6241 taactataaa cttcttttaa cgttttgttc tctagttctg ccattgtcaa ataatcgctc
     6301 attcagctcc cacttcctct atcatttcaa ttgatgcccc taaggctgtt agtttctcga
     6361 taatatgatc ataaccgcgt aagatataat ctacattgta aatcgtcgtt gtcccttctg
     6421 ccaatagtcc agcaatcact aaacaagcac ctgcccgaag atcagaagcc acgacttctg
     6481 tgccgtgaag tttatttgga ccattcacga tgatcatatt tccttccacg gtaatatcag
     6541 cgcccattcg tgctagttcc ggcacatggt tcacacgttt cgcatagatc gtatcgatga
     6601 tttctgaagt gcctgtagtc atcaatagta acgcagtaag tggctgctgc agatctgtgg
     6661 caaatcctgg ataaggatag gtcatgatgt ttgcgggttt taattctttc gaggggtaaa
     6721 cttcgatttc atcttcgctg attttcatat ggacaccaat ttcctgcaat tttgctataa
     6781 agctttccaa atgttcaaag atcacatttt tcactttgat accatttcct actgcagctg
     6841 ctaatgctag ataggttcct gcttcgatgc ggtcaggaat catgaaatgc cgacagccat
     6901 gtaatgtttc gactccttcg atacggatca catctgtacc cgctcctcga acttttgccc
     6961 ccatattatt cagtaaagtt gccacatcta tgatttccgg ttctcgtgca gcgttttcaa
     7021 ttactgtctg tccttttgct tttacagcag ctaacatcac attgatcgtt gctccgactg
     7081 aaaccacgtc catgaaaatc cgattgccac gcaatccttt attttctgtc cgtaaataca
     7141 tggcaccatg ttcatttgtc acttcagcac ctagagcttc aaatcctttg acgtgtaggt
     7201 caatcggtct agggcctaaa taacagccac ctggcaggcc tactaccgct tcgccaaatt
     7261 taccaagcag tgttcccata aaataataag aagcacgcaa actattgatt tttccgtgcg
     7321 gcatcggaat cgatacgact tctcgaggat cgatttctag tatgttgttg gaaaaatgga
     7381 cagttgcgcc catgatctct aaaatttcaa ttaatgaatg aacatcttgt atgtccggca
     7441 caccttccaa gatcactggg gaatcagcta agattgcagc tggaatcaat gcgactgcac
     7501 tattttttgc accgctgatc gttacttcac cagctaaagg tcggtttcct ttgattacga
     7561 tttttttcat ctgtttccac cttgtttatt aatctttttt catggatatg cttgtatttt
     7621 atcatagata aaaaaaagaa acgagcatgg tctgccgttt taagtaaaaa agaatggaga
     7681 gcgacttcaa aagtcgttct ccattcctat atatcgtatc tgttatgtgg atgcttacgc
     7741 tttgttagct gaaccgaacc agtcgatatg agatttagca tcttcaacga ttgctgcttt
     7801 acctggagct aataatttac gagggtcgaa tcctttacct tctttatctt taccagcttc
     7861 gatatattta cgagtagctg cagcaaatga taattggaat tcagtgttaa catttacttt
     7921 tgagataccc attgagatag ctttttcgat ttgatcttga ggaatacctg atccgccatg
     7981 taatactaaa ggcatgtctg gacctacagc atcagcaatt gcttgtaagt ggtcaaaagc
     8041 taatcctttc cagttttctg gatattgacc atggatgtta ccgataccgc aagctaagta
     8101 gtcgatacct gtagcaacca tttgtttgca ttcttcaata tcagctaatt cgccgttacc
     8161 gatgattccg tcttcttctc cgccgattga accaacttca cattcaactg agataccttt
     8221 tgcatgtgct ttttcaacaa cgtctttagc taatttcaag ttttcttcaa atggtagatg
     8281 tgaaccatcg aacattactg aagtatagcc taattcgata cattccaaag cagcttcgta
     8341 atcaccatgg tctaagtgga tagcaactgg aacagtgatg ttcattgctt caactaaatc
     8401 agtgattaaa tctttacaga ttttgtaacc acccatgtat ttagctgcac ccattgatgt
     8461 ttggataagt actggtgctt gttttgcttc agccgcttca aggattgctt gagtccattc
     8521 caagttgttt gtgttgaatc cgccgacagc gtaaccgcct ttacgagcag cttttaaaaa
     8581 ttcagctcct gatactactg gcataataaa attcctccta agttttgtaa aactaaattt
     8641 gttaagacaa ttcttaacca acgaccctat tttaccaaac atttcggtag ttttctacta
     8701 aaatcaatga aaatgaaaat tttcaagaag ttttttgcaa aaaataaaat atccaatagc
     8761 gctttaatcg aaaaaagtct tttgctttct atcttttttc ctacaaataa taacaaatca
     8821 gaaaaagaga aaagtgacaa gaaaattgct tatccccttc tcttgctact tttctctttc
     8881 tatcaagttc aaaaattatt ttgtttcgtg atctgctaat gatgctccga caaatgcttt
     8941 gattaatttt tgcgggcgat ttggacgaga aatcaattct gggtggaatt ggcatcccac
     9001 aaagaatttt ttctctggca gctctacgat ttctactaaa cggttatctg gtgagacacc
     9061 tgaaaagact agcccttgtt cttcaaacaa ttgacgatat ttgttattga actcatagcg
     9121 gtgacggtga cgttcttgca caacatcggc tccatcatat gcttcagccg tcttcgttcc
     9181 ttttttgatt ttacatgggt ataatccaag gcgtaaagtt ccacccaaat tctcgatgtt
     9241 ttcctgatca gccattaaat caataatatt gtttgctgta tctggattcg tttcagctga
     9301 tgacgcatct tctaatccaa cgacgttacg tgcaaattcg acacaagcca tctgcatacc
     9361 aagacaaata cctaaaaacg gaacgtcatt ttcgcgagca taacgaatcg cttcgatttt
     9421 cccttctact ccgcgatcac caaatccacc aggaaccaag attccatctg cgtctttcaa
     9481 acgttcttct acattttctc tcgttaattc ttgtgctttg atccaatcga tctcgatgtc
     9541 tgaattatag gcgaaaccag aatgtttcaa tgcttcaact actgagatat atgcatctgg
     9601 taactcgaca tatttaccga caagcgcaat ccgtactttt ttcttcaagt tcaagacacg
     9661 ttcttctaat tctttccatt ctgtcatgtc tgcttctggt gtatctagtt ttaaatggtc
     9721 gcaaacaatt tgatccattc cttgtgcttg taaaactaat gggatggagt aaagtgtatc
     9781 tacatctggc gactcgataa ctgcttctgg ttcgacatca caaaattgtg ccaatttgtt
     9841 tttcacgctt tgagaaacag gttgctctgt acgtaccacc aaaatattcg gttggatacc
     9901 taaaccacgt agctctttta cactgtgctg cgttggttta gttttcattt ctccagcagc
     9961 tttcaagtaa ggaatcaatg ttgtatggat atacataaca ttatctgtac cgacatctgc
    10021 tttcatttga cgaagtgctt caaggaatgg caaagattct atatccccta ctgttccccc
    10081 tacttctgta atgatcacat ctgaatcagt cattttagct gctcgcatga ttttttcttt
    10141 gatctcattt gtaatatgag gaataacttg aacagttgct cccaagtatt cgcccttacg
    10201 ttctttacgc aaaacttcag aatagatttt tcctgtcgtc acgttggagt atttgttcaa
    10261 attgatgtca ataaaacgtt cgtagtggcc cagatccaaa tctgtttcag caccatcatc
    10321 tgttacgaat acttctccgt gctgatatgg gctcatcgtc cccggatcca cgttgatgta
    10381 tggatcaaat ttttggatag ttacttttaa gccgcggttt ttcaataagc gtcctaaaga
    10441 agctgcaaca atcccttttc cgattgatga aacgacaccg ccggtcacaa aaatatattt
    10501 tgtcataagt tcaaaaactc ctttttaatt tatgtgcagg aaaagtctca gacaataaac
    10561 aaagctccct attcttcaat aagaataggg agccgaaatt tctgtaaact tctgaccctc
    10621 taaaagggtg cccaaggtgt ataatacaag ctgactaagg gaaagtcaag tactcaaatg
    10681 aatactatcc gcttacaatt ttttcttttt cgatatcctt tgagaaatta tttcttaagt
    10741 cctttatgag tgcaaaaaaa atttaaccca cataataagc ttgatataaa catagctaac
    10801 gttaaaccta agcaaatcag aaaatcgtta ctgaaaaatt ttaagtgaaa taatagataa
    10861 aaaaataaaa caaacattat gatagataat atcccaccta acaaagaact actttttcct
    10921 acttgcagca acagtttata tagcttatcg tagaaacaat ctttttcttt cacccttatt
    10981 tacctccttt acgtaaaaaa cgtagtccac agttctcgga tttttggcat atcttttcta
    11041 ttaaataata tcaaaaacaa aacaattcca ctaatttttt caaagatgac ctttttttca
    11101 acaaaagtgc cttgtaggga ttcattgaag gtaacgaatc acataagtaa aaaataagga
    11161 gatgcgacaa aagtcgtgcc tccttatttc taaataaacg gtgttctcct gccaactcct
    11221 cggtcacaag tcacaatatc catcaaacat gagaagctgt ttttgaaatt atttcttgtg
    11281 attcggagtt aaatggtagg ctcacaacct ctggataaac ggtattcaaa agccagtttc
    11341 tcggtattaa gccaaattta tgcaaaaatg tagaaagcca ttttcacaaa atttttctta
    11401 atactcgaaa ctaaacgtct ttttcaaaac ctcttggtaa acggtgttca aaagcttgca
    11461 tctgcggtaa taagttcagc caattcaaaa acaagaaaag ctgtttttgc ttggctattc
    11521 ttattacttg ttgcagggca gctttttcac aacctctcca ctaaacggtg ttcaatcagc
    11581 tgaccctcgg tattagctca aaaaacggca aaatatgagg aactattttt ccgttagttt
    11641 ctctaatact caggtcaaaa cgctgatttc acagcctcta attcacggta ttcctgtttt
    11701 aataaagttc tagatattgt tctctttccc actcggaaac agtttgtctg aatgccgccc
    11761 attcgagacg ttttgcttca acaaaatttg agaagatatg ttgtcctaat gcgtcaatca
    11821 ttacattatc cttacgcaat tctttgatag cattatggat agttgaaggt aagtcctgga
    11881 tatgtgcagc ttctctttct tcctcatcca ttacataaat gttacggtca acagctgctg
    11941 gcggtgtgat ctgattacgg ataccgtcta gtcctgcttg caacaatact gccattgcca
    12001 agtatgggtt tgcagatgga tcaactgacc gcaattccaa acgtgtggat aaaccgcgag
    12061 attctggtac acgtactaat ggcgaacgat tacggccact ccatgcgaca taaacaggcg
    12121 cttcataacc tggaacgaga cgtttataag aattgactgt tggattgcaa acggctgtgt
    12181 aggcacgagc atgtttcagc aaaccaccta agaaatgata agctgtttca cttagttcca
    12241 attcaccgtc tttatcatag aaaacatttc cttcatcatt gaacaaagac atattgcaat
    12301 gcataccaga accattgatc ccaaataacg gtttcggcat gaaagtcgcg tgaagtccat
    12361 gcttgcgtgc aatcgttttg acaacgagct tgaaggtttg gatattatca caagcatcta
    12421 ccacatctgc atatttaaag tcaatttcat gctgtcctgg cgctacttca tgatgtgaag
    12481 cttccacttc aaagcctaga ctttctaatt ccaacacgat gtcacggcgg caattttcac
    12541 ctaaatctgt tggtgcaaaa tcaaaataac cacctttatc attcaaggtc gttgtgatcc
    12601 caccgtcttc gtctaattta aataagaaga attctggttc aggtcctaaa ttgaaagagg
    12661 taaaaccaag gtcgcgcata tctttcaatg ctcgtttcaa attcccgcga ggatcgccgg
    12721 caaatggtgt cccatccgga ttatagatat cacaaattaa acgagcaact tttccatgag
    12781 cactttccca cgggaagatc atccaagtgg agagatctgg atacaaatac atatcgcttt
    12841 cttcaattcg aacaaatcct tcaatcgaag aaccgtcaaa catcatttta ttttctaata
    12901 ctttgtcgat ctggctcact ggaacttcta catttttgat cgtacctaag atgtctgtga
    12961 acatcaatcg taaaaatcgt acgttttctt cctcaatgat tcgtttgatt tcttcacctg
    13021 ttatctgttt ctttaccatg gtatccctct tcctttttta atttattcag tcaaagaatt
    13081 gtctttgcaa tccttgcgac tgataatgac ctgttttgct taacccacct tgcgccaaaa
    13141 tctcatcacg aaaaatttgt cgaacgtcat gatctgtcaa cggcttcttg ttttttgctt
    13201 cttgtttttt cgcctgtgcc atttcgtata cacgtttgat cccagccata ttcaatccct
    13261 ctgaaagata gtctttaatc tcaagcaaca tatcaatatc attcagcgaa tacatacgtc
    13321 ggtttccttc gcttcgttcc ggatgaacta aatcttgttc ttcataatag cgaatttgcc
    13381 gagcagacaa atcagtcagc ttcataactg taccaattgg aaatacggac atagatcggc
    13441 gcaattcctt ttctcccatc agacgtcccc tttcgtttta taagttggct aaagtgtacc
    13501 aattaaaaaa aatattgtca actgcatatg ttaggttttc taacatgaga tctctatttt
    13561 ttataaaata acaaaaaaat aagaacacaa aaaaggtatc tagttgattt tcgtaccaga
    13621 taccttttat tttttattat actgtactat cctttattag tgtttttatt
//
LOCUS       DACDEG010000067        13590 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.069, whole genome shotgun sequence.
ACCESSION   DACDEG010000067 DACDEG010000000
VERSION     DACDEG010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.069"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..197
                     /locus_tag="IV142_001954"
     CDS             <1..197
                     /locus_tag="IV142_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485609.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="IV142_001955"
     CDS             314..811
                     /locus_tag="IV142_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP8485610.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            786..1292
                     /locus_tag="IV142_001956"
     CDS             786..1292
                     /locus_tag="IV142_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8485611.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            1276..2781
                     /locus_tag="IV142_001957"
                     /pseudo
     CDS             1276..2781
                     /locus_tag="IV142_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
     gene            3526..5352
                     /gene="ltrA"
                     /locus_tag="IV142_001958"
     CDS             3526..5352
                     /gene="ltrA"
                     /locus_tag="IV142_001958"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8485612.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            5387..6403
                     /locus_tag="IV142_001959"
                     /pseudo
     CDS             5387..6403
                     /locus_tag="IV142_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            6406..8583
                     /locus_tag="IV142_001960"
     CDS             6406..8583
                     /locus_tag="IV142_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP8485613.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            8580..9581
                     /locus_tag="IV142_001961"
     CDS             8580..9581
                     /locus_tag="IV142_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP8485614.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            9578..10510
                     /locus_tag="IV142_001962"
     CDS             9578..10510
                     /locus_tag="IV142_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8485615.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            10755..10871
                     /locus_tag="IV142_001963"
     CDS             10755..10871
                     /locus_tag="IV142_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP8485616.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            10887..12806
                     /gene="tet(M)"
                     /locus_tag="IV142_001964"
     CDS             10887..12806
                     /gene="tet(M)"
                     /locus_tag="IV142_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP8485617.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNVCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            12925..13092
                     /locus_tag="IV142_001965"
     CDS             12925..13092
                     /locus_tag="IV142_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8485618.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(13152..13505)
                     /locus_tag="IV142_001966"
     CDS             complement(13152..13505)
                     /locus_tag="IV142_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8485619.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSIM
                     ESLASGINEARNIED"
BASE COUNT         4436 a         2449 c         2863 g         3842 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gactacgagt taccctttac
      481 ggttgacgaa tacacttcca ttggcgaact caatcgacta tgggaaatgg tatcggaatt
      541 acccgaagaa ttacaatcgg agctatctgc tctgctcact catttttcaa gcattgaaga
      601 actaagcgaa catcaagagg atattatcat tcattccgat tgtgatgata tgtatgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtaccagcta gtcttcaaaa
      721 ctatattgat tatcaagcct atggtcggga tttagacctt tcaggaacgt ttatctcaac
      781 caatcatggg atttttgaaa tcgtctatta aatctgtcgg tacattacta ctggcagatt
      841 ttctatttta cggggtggct caatcagcta cccctatttt ttatgaaagg attgattaca
      901 tgaagaaaat acgaagctat accagtatct ggtctgtgga aaaggtactg tattctatca
      961 atgattttag acttccgttt cccataacct ttacgcaaat gacatggttt gtcgtgtcac
     1021 tctttgcagt gatgatactt ggcaacttgc cccctctttc catgatagag ggagcatttc
     1081 tcaaatactt tgggattcct gtggctttca catggtttat gtctacaaaa acttttgatg
     1141 gtaaaaagcc ttatggattt ttgaagtctg tcattgctta tgcactgcga ccaaagctga
     1201 cctatgcagg aaaaaaagta acgcttggca gaaaccagcc acaagaagcc attacagcag
     1261 ttaggagtga attttatggc atatccaatt aaatacattg aaaacaatct cgtctggaat
     1321 aaagacgggg aatgttatgc ttactatgag cttgttcctt acaattactc atttctaagt
     1381 ccagaacaga aaatacaagt gcatgattct ttcagacagc ttatcgcaca aaatcgtgat
     1441 ggcaaaattc atgctttaca aatcagtaca gaatccagca tacgttctgc acaagagcgt
     1501 tccaaaaatg aagtcactgg caagctcaaa gcggttgcct atgacaaaat cgaccaacag
     1561 acagacgctt taatatccat gattggcgaa aatcaagtga actaccgttt ctttatcggc
     1621 tttaagttgc ttctcaacga tcaggagttt tctatgaaaa gtcttaccgt tgaagcaaaa
     1681 aatgctttgt ctgattttgt ctatgatgtg aaccataagc tgatgggcga ttttgttagt
     1741 atgagtaatg atgaaatcct gcgttttcag aagatggaaa agctcttaga aaataaaatc
     1801 tctcgtcgtt tcaaaatccg caggttagat aaggacgact tcggctatct gattgaacac
     1861 ctttacggac agacaggcac tgcctatgaa gagtatgagt accatctatc aaagaaaaag
     1921 ctggataatg aaacgctgat taaatactat gacttgatta agcctactcg ctgtttggtg
     1981 gaagaaaaac agcgatattt gaaaatccag caggaagatg aaaccgtcta tgtagcttac
     2041 tttaccatta acagcattgt cggagaactg gacttcccgt cctctgaaat cttctactac
     2101 cagcaacagc aatttacatt cccgattgat acgtcaatga atgtggaaat tgtagcgaat
     2161 cgtaaagccc tatctactgt ccgcaataaa aagaaagaac tgaaagactt ggataaccac
     2221 gcttggcaaa gtgataatga aaccagctcc aatgtggcgg aagctctgga aagtgtgaat
     2281 gagctggaaa ccaatttaga ccaaagcaag gaatctatgt acaagctgtc ttatgtggta
     2341 agggtatcag caaatgatct tgacgaactc aaacgtcgtt gtaatgaagt gaaagatttt
     2401 tatgacgatt taagcgtaaa actggtacga ccatttgggg atatgctcgg cttacatgaa
     2461 gaatttttac ctgccagcaa gcgttatatg aatgattata ttcaatacgt gacctctgat
     2521 ttcctcgctg gtttaggttt tggtgctact caaatgctgg gggaaaatga ggggatttat
     2581 gttggctaca gcttagatac tggacgcaat gtctatctga aacctgctct tgccagtcaa
     2641 ggggttaagg gttcagtaac caatgcgtta gcgtcggctt ttgttggttc gctgggtggt
     2701 ggtaaatcct ttgcgaataa ccttatcgtc tattatgcgg tgctttatgg ggcacaagca
     2761 gtgattgtag acccaaaagc aggtgcgaaa cgttcctact tgaaaagagt aggcacagca
     2821 cttcattaaa tcaatattta atgtgtgttg gagaactaaa cacacgaata gtcgaatgac
     2881 ggggtaacgc cctgaaaggc tactttaata ctctgactgg cagaaacttg agtaaacaag
     2941 gctctgtcag aagctcggtg aagtcggcag acagacaccg taacagataa tgctgacgaa
     3001 agattcccag aggtgggcgt tgtgtcctat atgccggaag tctataaaat atctatggtt
     3061 agaatgtaga tgagaggtct actgacgaat ccttgaatgt acaggtctat atcggggctg
     3121 gatagaaatg tccagtgaca caaacagtgt cgctactgtg ggtgagtaag actccgcatt
     3181 tatgaaaatc catataacgt taaaggcgtt atcaaggtag caggcttata gaggaaacct
     3241 aaggatatat gtacagatag tgtgtttgga acgttgaaag ctgacaacat ggagatttta
     3301 ctgtctgtga agtgttcaca tcagaaagtt attgtgagat tagcaatgat ataaggtgtg
     3361 ttaatgtcag tgagagtggt ggcacagtac ctatgaaacc gtgataacaa gcggtggagg
     3421 gatagccact agtcatatga agaatagaat tactaagaaa aatggtatgg gttcgagtaa
     3481 gactaagaga tgtacttgct cctgcaaaca aggagggtac agaccatgct aaagaaaaac
     3541 aagctccgct ataacgagta ttatgatatg caacatattt atgacgagtt atacgcacag
     3601 agcaagaacg gtaataactt ttataagtta cttgaaatta ttggttcgga acaaaatatt
     3661 tgtttagctt accgtaatct gaaatcaaac agtggcagta aaacggctgg aacggatgga
     3721 atgacgatag acgatataaa acaactcagc aatgctgaga ttgttgctac cgttcgggaa
     3781 agccttagca actaccgccc caaatcagtc agacgtgttt tcattccaaa agctgggagc
     3841 gataaaatgc gtccattggg gattccgtgt atctgggaca ggctggtaca acaatgtatc
     3901 ctgcaagtcc tagagccgat atgcgaacca aagttccata atcattccta tggattccgt
     3961 gcgaatcgaa gtgcccacca tgcagtcagc agagtaacga cactgataaa tttatcaaaa
     4021 tatcattact gtgttgacgt agacattaaa ggatttttcg ataatgtcaa tcatggaaaa
     4081 ctattaaaac aaatctggac acttggtatt cgggataaaa gattgatttg cattatcagc
     4141 aaaatgctga aagccgaaat tgacggcgag ggtgtcccag aaaaaggaac gccacaaggc
     4201 ggtctgttaa gtccactgct ctccctcata gtccttaatg aattagactg gtgggtaagc
     4261 agtcagtggg aaacattcca gccgaagaac cgcagtaaaa atgggtggct tcaatacgca
     4321 aagaaatata ccaaactgaa aagcggtttt attgtacgtt acgctgatga ttttaaaatc
     4381 atgtgttcaa cctatggaga agcacaaaga ttttaccaca gtaccgtaga ttttcttaat
     4441 aaaaggctga aattagaaat cagtccagag aaatcaaagg tagtaaatct gaagaaaaat
     4501 tcatcagatt ttctggggtt caaaatcaaa gtcattccaa aaggaagaac gaaacacggt
     4561 tatgttgcca aaacagatat gaaccaaaaa gcattaaaga aagcgaaaac aaatctaaag
     4621 ttaaaggtaa aagatatagc cagacatacg actggcttca atatcagcag atataatctg
     4681 acagttatcg gtatgcagaa ttactactgc atagcaacta acgtatataa caacctgaca
     4741 gaagtaagtt acgctctctt accgaccata cgaatccgtt taaggaacat tgcaaaatca
     4801 gttccatttg aaagtacaag ttcagaattt cagagcagga caaaaggaat cagaccaaaa
     4861 acaaaaatag tgatgattgc ggataatccg ctattgccga ttcaaggagt acaacacaaa
     4921 aatcctatga atttctcgca agatatttgt aattttacaa agcaagggag aaacaaggtg
     4981 catgaggacg ttgtagttgt aacaaaagaa gaaatccgag cgttgttgga aaatgaaaac
     5041 cctgccgata gcgtagagtt taacgacaat cgtatttcgg catatatcgc acaacaaggc
     5101 aactgctatg taatgaatcg tcgaggaact ccgtccactc tgatatgtat tcacaaatca
     5161 gacaaaggag ataaccttga cagatattct aatctggcat ttgtggaaat tcccattgca
     5221 aaggcaatcc taacagaatc agcagacgaa gcaaaatcac tgttaaaagg ttatgttctt
     5281 aatagtcagc agaagaaaaa actgaacaga atacggacga attacggata ccagacaata
     5341 actgggaaat aacttataaa tctatttgga tatgatggaa cgccgtatga agcgaaagtt
     5401 tcatgtacgg tgtgaagcgg gggaaaactt ggagataaca tcaaaaagtt acctatcgct
     5461 ataacgtggc agatggaaag aaaccttgcc agagatttcc catgaaatca atatcgtcac
     5521 tctgacttct gatgagaaaa acaaaggctt acttgaccct tatgtgatta tgaaaaatcc
     5581 caaagattct gaatcactgg ctattgatat tctgacattc cttacgggga tttcctctcg
     5641 tgatggggaa cgcttcccaa tccttagaaa agccattcgt gcagtaacca atagtgaagt
     5701 acgagggttg atgaaagtga ttgaggaatt acgggttgag aatacgccac taagtaccag
     5761 tatagccgac catatcgaaa gttttacaga ctatgacttt gcacatttat tattcagtaa
     5821 tggttatgtg gagcagtcta tcagcttaga aaaacaactg aacattatac aggttgcgga
     5881 cttggtactt cccgacaagg aaacttcctt tgaggaatat accactatgg agcttttatc
     5941 cgttgctatg ctgattgtca ttagtacctt tgctttagac tttatccata cagaccgaag
     6001 cattttcaag attgtagatt tagacgaagc atggagcttt ttacaggtag cacaaggaaa
     6061 aacactatct atgaagctgg ttcgggctgg tcgtgctatg aacgctgggg tatatttcgt
     6121 gacccaaaat acagacgacc tcttagatga aaaactgaaa aataacctcg gcttaaaatt
     6181 tgcatttcgt tccactgacc ttaacgagat taaaaagacc ttagcctttt ttggtgtaga
     6241 cccagaggac gaaaacaatc agaagcgatt gcgtgatttg gaaaacgggc aatgccttat
     6301 cagtgattta tatggtcgtg tcggtgtgat acagttccac cctgtatttg aagaactgct
     6361 ccatgccttt gataccagac cacctgtgcg aaaagaggtg taaatgtgaa accatcaata
     6421 gtaaacagaa taaaatcaaa ctggacgctg aaacgtctag gtaaagtggc aatgacagtg
     6481 gctttcacac ttgtgattgc catttttctt ttagccatgc tgggaacggt ggttcaagct
     6541 gcgggcttgg tagatgatac ggtcaatgtg gcaaatgaat acagccgata cccacttgaa
     6601 aactatcaac tggattttta tgtggataat agctggggct ggcttccgtg gaactggtcg
     6661 gacgggattg gaaaacaggt catgtatgga ctatatgcca ttaccaattt tatttggaca
     6721 atcagtttgt atgtttccaa tgcgacaggt tacttagtac aggaagccta ttccttagac
     6781 ttcatttccg ctacagcaga ttccattggt aagaatatgc agaccttagc tggtgtgagt
     6841 gcaaacggat tttcaacaga gggtttctat gttggattcc tcttactctt gattttggtt
     6901 cttggggttt atgttgccta tacgggactg ataaagagag aaaccacaaa ggcaattcat
     6961 gccattatga attttgtgct ggtgtttatc ctatcggctt cctttattgc ctacgctccc
     7021 gactacatta aaaaaatcaa tgacttttca tcagacatca gtaatgccag tttatcactt
     7081 ggcacgaaga ttgtcatgcc ccattccgat agtcaaggca aggacagcgt ggacttaatc
     7141 agagatagcc tgttttccat acaggttcag caaccgtggc tactgcttca atacaacagt
     7201 tcagacattg aaagtatcgg tattgaccgt gtggaaagcc tgctctccac cagcccagat
     7261 tccaacaatg gcgaagacag agaaaaaatt gttgcggaag aaattgaaga cagaagcaat
     7321 accaatctaa ccattacaaa gaccattaac cgtttaggta cagtcttctt cctatttgtc
     7381 ttcaatattg ggatttccat atttgtattc ctattaacag gaatcatgat tttctcgcag
     7441 gtacttttta tcatctatgc tatgtttctg cctgtgagct ttattttaag catgattcca
     7501 tcatttgatg gtatgtcaaa acgagccata acaaagctct ttaataccat tttgacacga
     7561 gctggaatca cattgattat tacgacagca tttagtattt caaccatgct ctatacctta
     7621 tcggctggtt atccgttctt tttgattgct tttctacaga ttgtgacctt tgcaggaatc
     7681 tacttcaagc tgggcgattt aatgagtatg ttttctctac agagtaacga ttctcaaagt
     7741 gtgggaagtc gtgtgatgag aaaacctcgt atgcttatgc acgctcacat gcaccgtcta
     7801 cagcggaaac ttggacgttc catgactact ctaggggctg ggtctgccat tgttacaggt
     7861 aaaaaaggac agtcgggttc ggggagttct gcaaggacac aagcagatca ctcccgacca
     7921 gacggaaagg aaaaatcaac acttggaaaa cgtatcggtc aaaccatcgg tacagtagct
     7981 gataccaaag acagaatggt agacactgct agtggtttga aagaacaggt taaagatttg
     8041 ccgaccaatg caagatatgc agtatatcaa ggaaaatcca aagtaaaaga gaatgtccgt
     8101 gatttaacca gtagtatttc tcaaaccaaa gcggacagag ccagtggacg caaggaacag
     8161 caggaacaaa ggcgaaaaac cattgcgaag cgtcgctctg aaatggaaca ggtcaaacag
     8221 aaaaaacagc ctgcttcttc tgttcatgaa agaccgacta caagacaaga acaatatcat
     8281 gatgaacaga cctcaaaaca gtctaatatt cagacttcat ataaggaatc tcaacaagcc
     8341 aaacaagagc gtccagcagt taagtccgat ttttcaagtc caaaagtgga acgccaaggc
     8401 aataccgttc aagaaaaaac cgttcaaaag ccagcaactt caaccactac agcagataga
     8461 acttcacaac gtccaatcac aaaagaacgt ccgtctactg ttcaaagagt accactacaa
     8521 aatacaagaa gtagaccacc aatcaaaacc gccaccatta agaaagtcgg taagaaacca
     8581 tgaagttgaa aactttagtg attggtggtt ctggattatt cttgatggtc ttctcactgc
     8641 ttctgtttgt tgccatttta ttttcagatg aacaggacag cggaatttcc aatattcatt
     8701 atggaggtgt gaatgtttcc gcagaagtgc tggctcataa gcctatggta gaaaaatatg
     8761 ccaaagaata tggcgttgaa gaatatgtca acatacttct tgcgattata caggtggaat
     8821 cgggcggtac tgcggaagat gttatgcagt cctcggaatc cctcggtctt ccacctaatt
     8881 cattgagtac agaagaatcc attaagcaag gtgtgaagta tttcagtgaa ttattagcca
     8941 gtagcgaaag gctcagtgta gatttagaat cggttatcca gtcctacaat tatggtggtg
     9001 gtttcttagg gtatgtggct aatcgtggaa ataaatatac ctttgaactg gctcaaagtt
     9061 tctcaaaaga gtattcaggt ggcgaaaaag tgtcttaccc caatcccata gccataccta
     9121 tcaatggggg ctggcgatac aactatggca atatgtttta tgtgcaactg gtaacgcagt
     9181 atcttgtcac aacagagttt gatgatgata cggtacaagc catcatggac gaagcactga
     9241 aatatgaggg ctggcgatac gtttacggtg gagcttcccc gactacttct tttgattgta
     9301 gcggactgac acaatggacg tatggaaaag ctggaattaa cttaccacga accgcacaac
     9361 agcaatatga tgtgacccag catatcccac tatcggaagc acaagctggc gatttggttt
     9421 tctttcattc tacctataac gctggctctt atattactca tgttgggata taccttggca
     9481 ataaccgtat gtttcatgca ggcgacccaa tcggttatgc cgacttaaca agcccctact
     9541 ggcaacagca tttagtggga gcaggacgaa tcaaacaatg agaaaggaag atttaatgat
     9601 gaaatttaga aaaaatcaga ataaagaaaa acagatacca aaggaaaaga aacctcgtgt
     9661 ctataaggtc aatcctcata aaaaggttgt gattgccttg tgggtacttt tagggcttag
     9721 tttcagcttt gcgatattca agcactttac agctatagat actcatacta ttcacgaaac
     9781 aactatcata gaaaaggaat acgttgatac tcatcatgta gaaaattttg tagagaactt
     9841 tgcgaaagtc tactattcat gggagcaatc cgataagtcc attgataatc gaatggaaag
     9901 tctaaaaggc tatctgacag atgaacttca agctctcaat gttgatacag tacgcaaaga
     9961 tattcctgta tcgtcttctg taagaggatt tcagatatgg acggtagagc caactggcga
    10021 caatgagttt aatgtaacct acagtgtaga ccagctcatt acagagggag aaaatacaaa
    10081 gaccgtccac tctgcttata tagtgagtgt ctatgtagat ggttctggaa atatggtact
    10141 ggttaagaat ccgaccatta ccaacatacc taagaaatca agttataaac caaaagccat
    10201 tgaaagtgag gggacggttg attccattac aaccaatgaa atcaatgagt ttttaacgac
    10261 gttcttcaag ctctatccta cagcgacagc cagtgaactt tcctactatg tgaatgacgg
    10321 gatattaaaa ccaatcggaa aagagtacat ctttcaagaa ctggtaaatc ctattcacaa
    10381 tcgtaaggat aatcaagtca cggtatcgct gacagtggag tatatcgacc agcagaccaa
    10441 agcaacgcag gtatctcaat ttgatttggt acttgaaaag aacgggagta attggaagat
    10501 tatagaataa caaatattgg tacattatta cagctatttt gtaatcacgt actctctttg
    10561 ataaaaaatt ggagattcct ttacaaatat gctcttacgt gctattattt aagtatctat
    10621 ttaaaaggag ttaataaata tgcggcaagg tattattaaa taaactgtca atttgatagc
    10681 gggaacaaat aattggatgt ccttttttag gagggcttag ttttttgtac ccagtttaag
    10741 aataccttta tcatgtgatt ctaaagtatc cggagaatat ctgtatgctt tgtatgccta
    10801 tggttatgca taaaaatccc agtgataaga gtatttatca ctgggatttt tatgcccttt
    10861 tgggcttttg aatggaggaa aatcacatga aaattattaa tattggagtt ttagctcatg
    10921 ttgatgcagg aaaaactacc ttaacagaaa gcttattata taacagtgga gcgattacag
    10981 aattaggaag cgtggacaaa ggtacaacga ggacggataa tacgctttta gaacgtcaga
    11041 gaggaattac aattcagaca ggaataacct cttttcagtg ggaaaatacg aaggtgaaca
    11101 tcatagacac gccaggacat atggatttct tagcagaagt atatcgttca ttatcagttt
    11161 tagatggggc aattctactg atttctgcaa aagatggcgt acaagcacaa actcgtatat
    11221 tatttcatgc acttaggaaa atggggattc ccacaatctt ttttatcaat aagattgacc
    11281 aaaatggaat tgatttatca acggtttatc aggatattaa agagaaactt tctgccgaaa
    11341 ttgtaatcaa acagaaggta gaactgtatc ctaatgtgtg tgtgacgaac tttaccgaat
    11401 ctgaacaatg ggatacggta atagagggaa acgatgacct tttagagaaa tatatgtccg
    11461 gtaaatcatt agaagcattg gaactcgaac aagaggaaag cataagattt cagaattgtt
    11521 ctctgttccc tctttatcat ggaagtgcaa aaagtaatat agggattgat aaccttatag
    11581 aagttattac taataaattt tattcatcaa cacatcgagg tccgtctgaa ctttgcggaa
    11641 atgttttcaa aattgaatat acaaaaaaaa gacaacgtct tgcatatata cgcctttata
    11701 gtggagtact acatttacga gattcggtta gagtatcaga aaaagaaaaa ataaaagtta
    11761 cagaaatgta tacttcaata aatggtgaat tatgtaagat tgatagagct tattctggag
    11821 aaattgttat tttgcaaaat gagtttttga agttaaatag tgttcttgga gatacaaaac
    11881 tattgccaca gagaaaaaag attgaaaatc cgcaccctct actacaaaca actgttgaac
    11941 cgagtaaacc tgaacagaga gaaatgttgc ttgatgccct tttggaaatc tcagatagtg
    12001 atccgcttct acgatattac gtggattcta cgacacatga aattatactt tctttcttag
    12061 ggaaagtaca aatggaagtg attagtgcac tgttgcaaga aaagtatcat gtggagatag
    12121 aactaaaaga gcctacagtc atttatatgg agagaccgtt aaaaaatgca gaatatacca
    12181 ttcacatcga agtgccgcca aatcctttct gggcttccat tggtttatct gtatcaccgc
    12241 ttccgttggg aagtggaatg cagtatgaga gctcggtttc tcttggatac ttaaatcaat
    12301 catttcaaaa tgcagttatg gaagggatac gctatggttg tgaacaagga ttgtatggtt
    12361 ggaatgtgac ggactgtaaa atctgtttta agtatggctt atactatagc cctgttagta
    12421 ccccagcaga ttttcggatg cttgctccta ttgtattgga acaagtctta aaaaaagctg
    12481 gaacagaatt gttagagcca tatcttagtt ttaaaattta tgcgccacag gaatatcttt
    12541 cacgagcata caacgatgct cctaaatatt gtgcgaacat cgtagacact caattgaaaa
    12601 ataatgaggt cattcttagt ggagaaatcc ctgctcggtg tattcaagaa tatcgtagtg
    12661 atttaacttt ctttacaaat ggacgtagtg tttgtttaac agagttaaaa gggtaccatg
    12721 ttactaccgg tgaacctgtt tgccagcccc gtcgtccaaa tagtcggata gataaagtac
    12781 gatatatgtt caataaaata acttagtgta ttttatgttg ttatataaat atggtttctt
    12841 gttaaataag atgaaatatt ttttaataaa gatttgaatt aaagtgtaaa ggaggagata
    12901 gttattataa actacaagtg gatattgtgt cctgtatgtg gaaataaaac acgattaaag
    12961 ataagggaag atactgaatt aaaaaaattc cccctctatt gtccgaaatg cagacaagaa
    13021 aatttaattg aaataaagca gttcaaagta actgtgatta cagagccaga cgcaaagacg
    13081 cagagccgat aaaatgagat taatacaatc tcattttatc ggctctttcc gttatgtatg
    13141 gattctttta attagtcttc gatgtttctt gcttcgttga taccgctggc taaagattcc
    13201 attatggata gttctttgtc tgtaaagcta tccatgtatt tctctatctg taatcgtcgg
    13261 gtgcttttta ccaagttatt agcaggtaag aaaaattcat caacggaaac atgaagtaac
    13321 gatacaaggt cataaagaac ttgtatgctg gggtgttgcc ctttattttc aatattagtt
    13381 aagtaccgtg ggtcaatttc aatcaatgct cccacttgtt cacgagttaa acctcgtttc
    13441 aatcgagctt ctttaatggc taaaccaaag gctctaaaat catatttatc ttctttttta
    13501 cgcatagtag accacctcta tacattttat tgttcctact gaattaaaaa caggtataga
    13561 aaaacgtgtt atatggttta taggtttata
//
LOCUS       DACDEG010000068        12922 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.071, whole genome shotgun sequence.
ACCESSION   DACDEG010000068 DACDEG010000000
VERSION     DACDEG010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.071"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            4..2376
                     /locus_tag="IV142_001967"
     CDS             4..2376
                     /locus_tag="IV142_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485620.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="IV142_001968"
     CDS             2437..3897
                     /locus_tag="IV142_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP8485621.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERSNGTYDFDRFYQGKGDINAQILFSYKAEQYYKGYKKHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3908..6112
                     /gene="topB"
                     /locus_tag="IV142_001969"
     CDS             3908..6112
                     /gene="topB"
                     /locus_tag="IV142_001969"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8485622.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSLLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKSQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYHKAAACTSEACDFRLWTTIAKKKLTATQLKEII
                     QNGRTSQPVKGLKGQKGSFEATIVLKEDFTTSFEFSEKKKTNYKKRTRRTTK"
     gene            6358..6954
                     /locus_tag="IV142_001970"
     CDS             6358..6954
                     /locus_tag="IV142_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8485623.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYSKAEWYELGKIEIKSRYGNPVIAYDPERTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTIRSYIMALN"
     gene            6967..7866
                     /locus_tag="IV142_001971"
     CDS             6967..7866
                     /locus_tag="IV142_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP8485624.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFENRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYEETMDSISNLMEKVI
                     HVEEKERKIAYQRHQQMNREMER"
     gene            7869..8354
                     /gene="ssb"
                     /locus_tag="IV142_001972"
     CDS             7869..8354
                     /gene="ssb"
                     /locus_tag="IV142_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8485625.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGILIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            8720..8980
                     /locus_tag="IV142_001973"
     CDS             8720..8980
                     /locus_tag="IV142_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485626.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9427..9633
                     /locus_tag="IV142_001974"
     CDS             9427..9633
                     /locus_tag="IV142_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP8485627.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9633..9857
                     /locus_tag="IV142_001975"
     CDS             9633..9857
                     /locus_tag="IV142_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485628.1"
                     /translation="MKTQHKLLNVKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFE
                     DLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            9872..10309
                     /locus_tag="IV142_001976"
     CDS             9872..10309
                     /locus_tag="IV142_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485629.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEAGVKPSTIKPSEYQALE
                     LTYGKFIETKKAKTVSSEWLDYFENVNKYGACYIMKKEDNENE"
     gene            10302..11009
                     /locus_tag="IV142_001977"
     CDS             10302..11009
                     /locus_tag="IV142_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485630.1"
                     /translation="MSNVVYQLTVEDVSHFLTNKEHLNEVVRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDCKRMPYQRFERKKGQ"
     gene            11390..11569
                     /locus_tag="IV142_001978"
     CDS             11390..11569
                     /locus_tag="IV142_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485631.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQELKQKYGKSESRIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            11662..11847
                     /locus_tag="IV142_001979"
     CDS             11662..11847
                     /locus_tag="IV142_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003027804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, HicA family"
                     /protein_id="HAP8485632.1"
                     /translation="MPLTGKEMLKLLKKNGWVERRQEGSHHHLYKDGVRITVPVHANQ
                     DLGRGLEQKILKDAGLK"
     gene            11881..12312
                     /locus_tag="IV142_001980"
     CDS             11881..12312
                     /locus_tag="IV142_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HicB family protein"
                     /protein_id="HAP8485633.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
BASE COUNT         4748 a         2183 c         2702 g         3289 t
ORIGIN      
        1 gacatggcag aagaaaaaga accactagac aatagtcgct tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaacgaa caaattgaga agatgtttga acaccagaga
      121 aagaccaacg gtcagccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gaacgtttag aaaacaaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacag
      241 gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcggc
      301 ctggatatga caatcgaaaa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgctacgatc cgaaaatacc aacggaaatt ggaaacatta
      421 gaacagctga aagaaagatc agaaaaagga aaagaaaatc ttcttcctga agtacaagca
      481 attattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 gactatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgcg
      661 ccaactgatt ctttgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt agaattgatc
      781 gatcaactca aaacgttgga taaccaatac gccgcagaag caggttatta taaattctat
      841 tttgaagaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaacccat ctatttagag ttagcaaaat tgtgcgaaca agtacctgaa
      961 aaggaacaaa aacaaacggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taagcaagaa
     1081 gtcaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa
     1141 gacgtgaaag aacttttgaa ttttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc tgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaaggat tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctacacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt
     1501 ccgaaccgtg tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacggggg gcgtatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac ttcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagcg
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tgtagaaacg gcagagaata agaatattgt gcccgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtgat tttaaaagga
     2221 aaaagttatg gaaaagagat tcaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 ccattccgat tttcacttga aacagtgtca gaagtggcca aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgc gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt ttgaaaatga ccgaaaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagacta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac tctaagcaac aatttaaagc agccacagat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtagaaa atcttcattt tgagacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gccgaactaa agccaaaaca tgaacggcaa aatttcttag ttcgagacga tcgtttagat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaatg agacagtagg ggcgagttat
     3001 caaggaacgt tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggctttaac ctaaagatcg gtgaccctaa gcagttaaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac tgaatcgtga tcagttaaac
     3181 gatacgtggt tagtttcgat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccatcga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagttgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aagtcattta tgaagaagtc gcaaaagaag aaaagatcac accagaagct
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaatt ggtttccgct
     3541 cacaaagtaa atgcgtcttt tggtcagcag ctttctgtta acgattcaat agaagctatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aagcttgtga acgctcgaat
     3661 ggtacgtacg attttgatcg tttttatcaa ggaaaagggg atattaatgc tcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa agcatgaaca tgaatttgta
     3781 cctgaagtga aaaaggattg gaacgatcaa ttgaagcatg agatccagca acaagaaatt
     3841 agaaagcaaa aacgtgcgat gttgtttcaa caaggcagac aacaagaaag ggagtgagat
     3901 cgtcacgatg aaaactgtca ttttagcaga aaaaccgtca caagcgaagg cttatgccga
     3961 tagcttttct aaagccaccc gaaaagacgg gtattttgag atccaagatc gattgtttcc
     4021 tggtgaaact gtgattacgt atggttttgg tcatttagtc gagttagatt cgcccgatat
     4081 gtacgatgaa aattggaaac aatggtcatt agaacattta cctattttcc ctacacatta
     4141 tcactatcat gttcctaaag ataagaaaaa gcaattcaac gtggtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgc gaaggggaac tgatcgcttg
     4261 gacgatcatt caacaagcag gcgccgatca tggaaagata tttaaaagac tttggatcaa
     4321 ctccttagaa aaagaagcca tttatcaggg attccagcag ttacgagatg cagaagagac
     4381 ctacccaaaa tttgaagaag cacaagcacg ccagatcgcc gattggttga tcggcatgaa
     4441 cggtagccta ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa gtttttcctt
     4501 aggcagagtc caaaccccaa cgctttacat gatttatcaa ttacaggaaa agattagaaa
     4561 cttccaaaaa gaaccatatt ttgaaggaaa atcccaagta atcgctcaaa atggcgcatt
     4621 tgatgcgaag cttgatccga atgaaaccca agcgacgcaa gaagcatttg aagattattt
     4681 aaaagaaaag ggggttcaat tgggaaaaca gccaggtacg atccaccaag ttgaaaccga
     4741 gaaaaaaagt gcggcaagtc cacgtctttt ttctctatcc agccttcaat ctaaaatgaa
     4801 tcagctcatg aaagctagtg ccaaagatac tttggaagcc atgcaagggt tgtatgaagg
     4861 aaaatatcta agctatccgc gaacggatac cccttatatt acggaagggg aatatgcgta
     4921 tctgttggat cacttagacg aatacaagca ttttttaaaa gccgaagcga ttccgactcc
     4981 tattcataca ccgaatagtc ggtatgtgaa taataagaaa gtccaggaac attacgcgat
     5041 cattcctaca aaaacggtta tgacagcagc ggcttttgaa caactaagtc cactgcagca
     5101 ggctatttat gaacaagtgt taaaaacaac ggtggccatg tttgcggaaa agtataccta
     5161 tgaagaaaca acgatcttga cgcaagttca gcagcttcag ctaaagacag tcggaaaagt
     5221 accagtagat ctaggttgga aaaagctgtt tggcaaagag agcgacagaa aagaaaagga
     5281 agaagaaccg ctacttccta aagtgacaaa aggagaagcg gtgacggtag atctacaagt
     5341 ccttgaaaaa gaaacgaagc caccacaacc ttataccgaa ggtacgttga tcacagcgat
     5401 gaaaacagct ggaaaaaccg tggatagcga agaagcacaa tccatattga aagaagtaga
     5461 aggaatcggt acagaagcaa cacgagcaaa tattattgaa actttgaaac aaaaggaata
     5521 tatcaaggta gaaaaaaata agcttgtggt gacaaataaa gggattttac tttgccaagc
     5581 cgtggaaaaa gagccgctgc taacaagtgc cgaaatgacc gccaaatggg aaagttactt
     5641 attaaagatt ggtgagcaga aaggaacaca agctactttc ttagctaata tccaaaaatt
     5701 tgtctctcac ttgttggaag tcgtaccagg acaaatccaa tccactgatt ttggatcgac
     5761 gttacaagag gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt tagggatttg
     5821 tccaaaatgc caggaacaag aagttttact ctatcataag gcggccgcat gtacgtctga
     5881 agcttgtgat tttagattat ggacaacgat tgccaaaaag aaactcacag cgacacagct
     5941 gaaggaaatc attcaaaatg gccgaacaag ccagccagtg aaaggattaa aaggtcaaaa
     6001 gggttcgttt gaagcaacga ttgttctaaa agaagacttc acaacgagct ttgaattttc
     6061 tgaaaagaaa aaaacgaact ataagaaaag aacacgccgc actacaaaat aatggagggg
     6121 ttatcttatg cgaaatacaa cacacaaaca acaagaaatc gaaaatcaaa aagcaaaaat
     6181 tcgttcagaa caagagaaat tacgtgccag taaaagaaga cttcagcaat taaagaacca
     6241 gcaacggatt caaaaagaaa ttgaattaga tcgataaaat cttcctggct attgtatacg
     6301 cataaactta tgttatacta tatttataag tttatacgta tacaaaggag ggagaaaatg
     6361 acaaaagcaa tggtttacaa gctgctagaa gaatttaacg gaactttggc tttgaaggat
     6421 gccaaaaaag cgggcatttc tcctgtaacg atcaaacgta tggtagatcg tggagagctg
     6481 gatcgtgagt atccaggttt tttcacctta cctggtcaat ttcctgatga attgtttatg
     6541 gcacaaaaga aatatgagcg cggcattatt tcgcatatca ccgcgttgga tttatatgat
     6601 ttaacggata tgatccctag acagatcgat ctaaccgtac cttacggtta tcacgtttct
     6661 gaaaaggggt tgaaagaatt cgcagttgag ttacactatt caaaagcgga atggtatgaa
     6721 ttaggtaaga tcgaaataaa gagtcgttat gggaatcctg ttatagctta tgatccagaa
     6781 agaaccttgt gtgacatttg gaatccctgg tacaacgttg aggatgaaat caaagtaaaa
     6841 gcaatcaaaa actacatgga gtcggacaga aaaaatctta gaaaattgaa tgagtatcga
     6901 cgaattttgc ctacagacaa aacgatacgc tcttatatta tggctttaaa ttaaaggaga
     6961 agacacatga cacctacgca attaaaagca aaagtgaaga atatttccag agaaaaagaa
     7021 gttgatccgc agcttgtttt aagacatttt atgatggaaa aattcttgga aaaaatctca
     7081 gagtctcctt atcgtgaaaa ttttgtgtta aaaggcggct tcttaattgg ctcaaagtat
     7141 ggcattgaaa atcgaacaac aaaagacatt gatacgactt tacgggaaat gaaagtaacc
     7201 aaagaaacat tgacaacggt tctaaacgat attttttcaa cgccgacgaa agaaggcatt
     7261 caatttgaga ttcaaggaat gaaagaaaca agggaagctg attattatcc agggtttagc
     7321 ttacgtgtac tggcacattt ggaaaacatg cggccagatt ttaaggtaga cgtgacgaca
     7381 ggtgactcga tctatcctgc aacgatcact cacagtcata aattgatgtt tgaaaatcga
     7441 acgattcctt tggaatctta tcctacagaa caaattattg ccgaaaaatt aagtgcaacc
     7501 tttgattttt taacagataa ttcgagagga aaagattttt atgatctcta tactattcct
     7561 aaaatggaaa agatcgatga gaagaccttg aaagatagtg tgagaaatac atttacacgt
     7621 cgaggaaaaa ctgatccgtt aaagatttat tacgaaaagg atatggatgt actgaaaaat
     7681 agtcatgagc taaaagatac ctggaagaaa tatcaaaaaa gcaatcctta tgctgcgtcg
     7741 atcacgtacg aagagacaat ggattcgatt agtaatttga tggaaaaagt gattcacgtc
     7801 gaagaaaaag agcgaaaaat agcttatcaa agacaccaac agatgaacag agaaatggag
     7861 agataaaaat gatcaataat gtaactttag taggacggtt aaccaaagat ccagatttac
     7921 gttataccgc aagtggcaca gcagtggcta cttttaccct agccgttaat cgtaatttca
     7981 cgaatcaaaa cggcaatcga gaagctgatt ttatcaattg cgtgatttgg cgcaaaccag
     8041 cagaaacgat ggcgacatta gctaaaaaag gcatcttgat cggcgttgtc ggacggattc
     8101 aaacacggac ttatgacaac cagcaaggac aacgtgtgta tgtgacagaa gttgtagctg
     8161 ataacttcca actattagaa agcaaagcag cgacagagag ccgtgcgcac gctgatcaat
     8221 cgtccacgag tccaagcacc accacttttg aacaacggga tacagcgacg cctaacaaca
     8281 atggattgaa cgcttcacag aatccatttg gtggacaatc aatcgatatt agcgatgatg
     8341 atctaccgtt ttaagtagca ttttctattg gttgcatttg ctacggttta tgttataatt
     8401 tagtcaacaa aaagaatttt caatctataa ccaaaaacca ctagctggcg ggctagtggt
     8461 tttcttttac gtttctatta taagaattaa cgagtaggct tactgtcttt cttggcaaat
     8521 ttacaatcaa gccatttgca gatatagtgc gcggctactt ctgccatgat tgcaaaaaca
     8581 aaatcaaaaa gaattttcaa acctataacc tcctcccctt gccagattag gccggggtag
     8641 cagacagcaa gactattata gcataattga aatatccaaa tgattggatt tttgctataa
     8701 ttttagcgag gtgattgtga tgaacaaata tgttggtttg cttgataaaa tccgagtaat
     8761 aaaaacgcaa ccgttattgg ttcgctttac tttacaaacg attcatgaat cgattaattg
     8821 tgtagttgca gatattgaaa ttatcgataa attgctaatt atggatgatg gaaaatacaa
     8881 tattgccgtc acaggtcact tcaataaacg aaatcagctg gttattgcta gcatgtatgt
     8941 tagaaatccc gatcatttta cacgatcgat gggcatataa ataaaagaag agttgtagtc
     9001 ctaaatgagg attgcagctc tttttgtttt acatagaaac gggaacagga aaaggcctgt
     9061 ttaggaaggc acaagcgcct tctcaattaa aaaacctttc tctcactcca accgccaccg
     9121 ccgttcgaga agaaaggaag tgaacgctgc acgttcacta agatatggtg tcttgcaaag
     9181 caagacaaaa taggaaaagc ttcctctcac caaaaggttc gttcctgttc gctatctctt
     9241 tattgatttg ccagaccggc aacaagttgc cttttatcac caaaaagcga caaggacaag
     9301 ggtaaatggc acgctgcgcg cccttgtcct tgtcgctttt cgttgattca ttctggccaa
     9361 caaatcaata aagaaagaac taacgaaagg aagtaaaacc tttcctaaga ggaggcagaa
     9421 tcaatcatgc gaaagaaatt tgattattgg ggtgttccct tcagcgaatt attcccaaac
     9481 tatcatgcac cacatactgt cgaatgcgat tgtggagaaa gagcgaaatg tatcaaaagc
     9541 tatcgtttgt atcaatgtcc aacatgcggg aaaaaatata ccttgtctta tggcgattac
     9601 attttgattg atgagaaagg aaagaaacgc tgatgaaaac acaacacaaa ttattgaatg
     9661 taaaagaagt catttctgaa aaagtagcag aagctttact tattccgcgg tactggacga
     9721 tagaaacccc tttagtagag gacgcaccga ctatgctatt tgaagaccta gtgaagattg
     9781 caggatatga actgcattat caagaaaact tggaaactta tgaaaacgaa tattggttaa
     9841 ccaaattaga gaactaaaaa ggagaaaaaa tatggctcag aattactatg acgaatttgt
     9901 caaattacct ttggataaaa tggcacaaaa aatggaggat atgacgttcc tataccatga
     9961 aacacgtgta cccaaaaaac attacaagga aaaattgtca gtcgcagtgg aagaaatgat
    10021 tgaatcaggt gtcgaaatga atctcattgc gacgtactat cgaacattgg aagaactgaa
    10081 aaaacaaaat ggaaaatggt ttttccaagc cttactatgc ttggaagcag gcgtcaaacc
    10141 aagcacaatc aaaccttctg aataccaagc tttagaacta acgtatggaa aatttatcga
    10201 aacgaaaaaa gccaagactg tttcaagtga atggttggac tactttgaaa atgtcaacaa
    10261 gtatggcgca tgttacatca tgaaaaagga ggacaacgaa aatgagtaat gtggtttatc
    10321 aactaacagt ggaagatgtt tctcactttt taacaaacaa agaacatttg aatgaagtag
    10381 tcagacggat tcaaaaagac tttagttgta tggaagaaat gaagtacttt attcaaagag
    10441 tggaagaaca gttgtatatg gaaagcgaaa cgagtcaaac gaaggtcaca gtcagccata
    10501 atcatttaga cgtgtataaa cgtcgtcctg aaacggtaaa aaaaggaaca gagcttctac
    10561 gactagaaac agaaaaatac atcatgaatg tgtacctaga aaggagtgag ctggtcgaaa
    10621 actttgtcag ctacacgatt tgtaggaaac actattactc agaatcagaa tattattcaa
    10681 tttttgagag catggaatta atggaagaag aaatggaaga gctaaacagc aaaaatgcga
    10741 cggtagacga ttattacctt gtatttacta gatttctgac gtgtgggacg gaatctatcg
    10801 tgttaatggg aatggattat gaactcgctg tacccgaagc atttttgagg gaatatgtag
    10861 aagaagtaga taacccaggc gacgactggt acacgacttt aactgatttt ttagatacct
    10921 acacttacga cgatacagaa gaaatccaaa atgagcttga ctgtaaacga atgccatatc
    10981 aacgatttga aaggaaaaaa ggtcaataac catttatacc gaaaagtgag aatgtgtgaa
    11041 ataagaacaa aaaaccttga aattaaggga atggttctta tttcgcacca atcgctccaa
    11101 ccgcccccgc cgctcaggtg gtgcgaaata agcgggcaac gaccgataac ctaagagaaa
    11161 gaaggaaatc catatgaata aaatcattcc tctgttgttc gtactgtttg ttggcgagat
    11221 acttatgcgt catgctgcag ctgtacattt aaacttcgta gtattcttag gaatactggt
    11281 attttgtttg atcttttggc gatttgtatt agcccttgtg agtctactat tcaagatgat
    11341 tgcagccata tttttcatag ggttatttcg atgaaaggag agagtactaa tgccaattgc
    11401 aataggaaat aaacgtttac ccgtgacgtt ggatgaaaag agacaaaaag aattgcagga
    11461 actcaaacag aagtacggca aaagtgaatc caggattatg tgtattgcgt tagatttatt
    11521 gattgcacaa gaaaaagcag ggtttgatgt accagcactc aaaaagtgac gtcacttttc
    11581 atttgaagaa acaaaagtga tagcacttta cgcacgtgtc gatacgtgtt atgattattt
    11641 tgtaaggagg tatagtgcac aatgccactt acgggaaaag aaatgcttaa gttattgaaa
    11701 aaaaatggct gggttgagcg aaggcaagag ggatctcatc atcatcttta taaggatggt
    11761 gttagaatca ctgttcctgt tcatgccaat caagatcttg gccgaggact tgagcaaaaa
    11821 attttaaagg atgcgggatt gaaataatct cgcattcgat taaagaaagg attgactgaa
    11881 atgttaagtg agcctttagc aaaaacctat cctgctattt ttagcccaga agaaggtggt
    11941 gggtacttta ttgaatttcc tgatgttcaa ggtgcttaca caggaattaa cgaagacgat
    12001 atatcttatg gaattgctat ggcacaagag gtgttaggta tggttttagc agattatatt
    12061 gagcatgaag atctacttcc agaaccaaca ccaatcaata agatttctgt tgaagatgat
    12121 tcttttacta ctttaattcg tgtagatgtt gccaaatatt tgaaagacac agaacttgtc
    12181 aaaaaaacct taacaattcc tcaatgggcg gataagctag gcaaaagggc tggcataaat
    12241 ttttcagtcc ttctaacaga atcaattgca gataaagcag ataacattct tcgttctgga
    12301 agaaataatt agaactcaaa agagtcagaa acttaaaaat ctttggcttc tttttttact
    12361 acaaagatgg cttctttaaa ttatttgcct gattgattta aaaaaattgc gcaaagacta
    12421 cgagctaatt aagttgctcg tggtctttct ttatgaaaac tttttagatc tagataaaaa
    12481 cattgagaca ctttgaaaaa tttttttaat agtaatgggc gcttctatgt caagctgatt
    12541 cttctttcgg taaattaatt ttacttagaa tatcactatc ttgataaaaa atggtgtatt
    12601 cacaaattct ggcataaaaa atacctactt tctagttaag gttcctgaat aagtcgcaat
    12661 ccaattattt ccagctgatt tataactttt taaatataat tttccataat accaataacc
    12721 attacttttt tctgtccaat ttcgttattg agaagatcta gaaggccagc caactttatc
    12781 aaaaatcatc attcctgtca ctcgtatcgt tttagaagct gctcgagtaa gtaatggaga
    12841 aactgttaaa taattttaag taacagttaa gtcgttagtt gggttatcaa tggctattcc
    12901 gtccttatct gaacctcgaa ac
//
LOCUS       DACDEG010000069        12416 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.073, whole genome shotgun sequence.
ACCESSION   DACDEG010000069 DACDEG010000000
VERSION     DACDEG010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.073"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            526..2337
                     /locus_tag="IV142_001981"
     CDS             526..2337
                     /locus_tag="IV142_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8485634.1"
                     /translation="MNENGEFEESKKYQATSEEEKAAVRELLEEAVPTDFLKEKENDH
                     FYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYYKFYFEEVKNGEVV
                     DQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKEQMQSKETKTKQKG
                     YQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFPERSFRNQLLIEEQ
                     FPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGFVREIEATPEDKKR
                     IESGQIEVIKKTYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQLDKKGQSELQAGIQ
                     AVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNTRNTPTQHLATSIH
                     ELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTLEKAIPYIAAWTKNGQTLDDK
                     EAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQEHKNTVETAEQKNI
                     VPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSE
                     EFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2398..3867
                     /locus_tag="IV142_001982"
     CDS             2398..3867
                     /locus_tag="IV142_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3991 domain-containing protein"
                     /protein_id="HAP8485635.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVGDDRLDHAVAFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFN
                     LKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRR
                     KQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKAT
                     YEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKE
                     ACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEF
                     VPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3878..5887
                     /gene="topB"
                     /locus_tag="IV142_001983"
     CDS             3878..5887
                     /gene="topB"
                     /locus_tag="IV142_001983"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8485636.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKRSEGFI"
     gene            6233..6829
                     /locus_tag="IV142_001984"
     CDS             6233..6829
                     /locus_tag="IV142_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8485637.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            6842..7741
                     /locus_tag="IV142_001985"
     CDS             6842..7741
                     /locus_tag="IV142_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP8485638.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKDGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            7744..7875
                     /gene="ssb"
                     /locus_tag="IV142_001986"
     CDS             7744..7875
                     /gene="ssb"
                     /locus_tag="IV142_001986"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8485639.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            7897..8229
                     /gene="ssb"
                     /locus_tag="IV142_001987"
     CDS             7897..8229
                     /gene="ssb"
                     /locus_tag="IV142_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8485640.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            8251..8508
                     /locus_tag="IV142_001988"
     CDS             8251..8508
                     /locus_tag="IV142_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485641.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(8667..8789)
                     /locus_tag="IV142_001989"
     CDS             complement(8667..8789)
                     /locus_tag="IV142_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP8485642.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            8979..9203
                     /locus_tag="IV142_001990"
     CDS             8979..9203
                     /locus_tag="IV142_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485643.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
     gene            9646..9852
                     /locus_tag="IV142_001991"
     CDS             9646..9852
                     /locus_tag="IV142_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP8485644.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9852..10103
                     /locus_tag="IV142_001992"
     CDS             9852..10103
                     /locus_tag="IV142_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485645.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEVLLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            10284..10556
                     /locus_tag="IV142_001993"
     CDS             10284..10556
                     /locus_tag="IV142_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485646.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            11001..11180
                     /locus_tag="IV142_001994"
     CDS             11001..11180
                     /locus_tag="IV142_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485647.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            11239..11595
                     /locus_tag="IV142_001995"
     CDS             11239..11595
                     /locus_tag="IV142_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485648.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT         4587 a         2043 c         2579 g         3207 t
ORIGIN      
        1 ttgggaaaat ccaaaagaaa attagtacga ttgcaaaatg aattaaacga acaaattgaa
       61 aagatgtttg aacaccagag aaagaccaac ggtcaaccaa tgaacgataa gagaaatggg
      121 cattcttggt ttaggcaaca agagcgaatc gaaaataaag tgcatagtct gagagaagag
      181 atcaagcaac aagaaaaaca agtagaaaaa cttgaaagac aagaagagat aaaggaaatg
      241 ggctataaca agtacggcgg actggatatg acgatcgaga atattccaag aatcaaagaa
      301 gaaatcgaac gctttgaaaa aggtgaatcg actttttcag ccgctacgat ccgaaaatac
      361 caacggaaat tggaaacatt agaacagctg aaagaaagat cggaaaaagg aaaagaaaac
      421 cttcttcctg aagtacaagc aatcattgac agcggacgag tgacgcaatg gaaaaaaaat
      481 ccgaccattt attttctgaa aggctatcga aaagtcgctt tagaactgaa tgaaaacggg
      541 gaatttgagg aatctaaaaa ataccaagcc acgtcggaag aggaaaaagc agctgttcgt
      601 gaattacttg aagaggctgt accaactgat tttttgaagg agaaagaaaa cgatcatttt
      661 tatcgagtag aattcaacga aaatgatcca gaaaaaggat tgaaaagtta tggtggggag
      721 atcgtaacat tcgaattgat cgatcaactc aaaacgttgg ataaccaata cgccgcagaa
      781 gcaggttatt ataaattcta ttttgaagaa gtgaagaacg gagaagtagt cgatcagatc
      841 cgaatggatc taggggatgg cgtcaacagt aatcaaccca tctatttaga gttagcaaaa
      901 ttgtgcgaac aagtacctga gaaggaacaa aaacaatcgg tcaaaaatga acctgtttcg
      961 aaagaacaaa tgcagtcgaa agaaacaaaa acaaaacaaa agggctatca aaaacgaacg
     1021 gcagaagaga ttaaacaaga agttaaatcc ctttcgcaac aggcactgga tgcggtgaaa
     1081 aagcatactc attctcctga agacgtcaaa gaacttttga actttatgtc tcgttttcca
     1141 gaacgatcct ttagaaatca attattgatt gaggaacaat ttccaggtgc aacggcttgt
     1201 ttaggtcgtg ccgcgttgaa aaaagaagga atctatatca aaaaagggga acaagggaac
     1261 aagatctttg tcagaaaaac ggtcaaagga ttttatgaga aaggacgtgg ctttgttcga
     1321 gaaatagaag ctacaccaga agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc
     1381 aaaaaaactt actacacgat tgaaaaagtg tttgatatca cacaaacaca gttaaaacct
     1441 gaagactatc cgaaaatctt tccgaaccgt gtctttgatt ttcaattgga taaaaaagga
     1501 cagtcagaac ttcaagctgg gattcaagcg gtagccaaca atattggaat acaaattcga
     1561 gatatgacag aaagtgaagt ctatcaaaga gaattaggac aagcacgtgg ggcgtatgtt
     1621 cgtaacgaat ttactggcga agaagaaatc gtcatgaata ctcgaaatac tcctacgcag
     1681 catttagcta caagtattca tgaattagcc catgcaagaa tgcacaaatt ttcagaatta
     1741 gataccgcaa ctaaagaatt gcaggcagaa atgacttctt atattgtgtg caaacatttt
     1801 ggaatggaca cgttggagaa agctattcct tatattgcag catggacgaa gaatggtcag
     1861 acgttggatg ataaggaagc tgcggaacgt gggaaaatca tgaatgatgt cagccgtgtc
     1921 gcaaatgaat tcattcaaac catttctaat gaaatcaatc aatacagaga aagagaacca
     1981 gagatccaac cagcagaagt gaagcaggag cataaaaata ctgtagaaac ggcagaacaa
     2041 aagaatattg ttcctgttgg ttcactttgg atcgacaaga aagacgggaa agtaatggaa
     2101 gaagatacgg gtcgaacgtt acatttgaag gatgcggcgt ttattgaaga tccacagcat
     2161 aatcaagtaa ttttaaaagg aaaaagttat ggaaaagaga tccaaacggc gttaccttca
     2221 gaagaatttg cgctaggaaa ttcattccga ttttcacttg agacagtgtc agaagtggca
     2281 aaacgaaaaa atgaaatcta ttatgaacaa caattgtcac aaaaagaacg tgtataaaac
     2341 aagtaaaaaa gactatctga aaaacggata gtcttttttc ttttgggagg ttttaaaatg
     2401 accgagaaaa aaatgagtct tattgatcga tgtaaacaga tcgatatagt ggattttgct
     2461 cgaaacaatg gaatggctgt agtcaataaa ggacgagact atcgcttaga agaccatgat
     2521 tcatttgttt ttgatcgaag aaaacaacgt ttttattgga acagtcaaaa tattagtggc
     2581 gacattattg aattagctaa actttttttc atagacaaag agatccaaga ccctaagcaa
     2641 caatttaaag cagccactga ttttattcta aagaatgaag acaaaacaga acgagtggaa
     2701 aatcttcatt ttgagacaga aaagtacaaa gatcaccccg ttgattatca accactcaca
     2761 gaaaaaggaa ggaattatct gaaggaagaa cgaaaactac cagagtggct gattgattat
     2821 gcagaaaaag aagggttgat tgctgaacta aagccgaaac atgaacggca aaatttttta
     2881 gttggagacg accgtttaga tcatgcggtt gcttttttat ggaaagatcc acagacagga
     2941 gagacagtag gggcaagtta tcaaggaacg atcgtcgatt ttaatcgttt cggtaaacgg
     3001 ggaacgtata aacacattga caagaatcct acgccaaatc acggatttaa tttaaaaata
     3061 ggcgatccga agcatttaaa attctttgaa agtagtattg atctgttaag ttacgctgca
     3121 ttgaatcggg agaaactaca agatgcctgg ttagtttcga tggacgggtt aaaacatcat
     3181 gtaatcagtc attacgttga agagtcgatt tctgaattaa gacgaaaaca aacatttcct
     3241 caatcgattg aaatatgtgt ggacaatgat cgtgcaggac atatttttta tgaaaaagaa
     3301 cagatgaagg gaatcgttga tccttttacc aataagaaaa tccgttgtga gcgaggaatt
     3361 ccaaatgatt ggcaagtacc taaagaatac aaagctactt atgaagcagt cgcaaaagaa
     3421 atgagtgttg aacctgaagc aattatggcc atacacaaaa ctgaaactaa tttacagctg
     3481 acaaatcaat tggtgtctgc tcatgatgtt caatctacgt ttggaaagat gttggcgaag
     3541 ggcgaaccag tagaaacaat tgatttgaaa gaagcatgta ccacggtcgc gaaggaactg
     3601 aaagtatgtg aacgcgcaga tggaacatac aactttgatc gtttttatag tagaaaagcc
     3661 aacataaaag acgttaatgc aggaatcctt ctttcctata aagcagaaca atactacaaa
     3721 ggctataaaa agcatgaaca tgaatttgta cctgaagtga aaaaggattg gaatgatcaa
     3781 ttgaagcatg agatccagca acaagaaatc agaaagcaaa aacgtgcgat gttgtttcaa
     3841 caaggcagac aacaagaaag ggagtgaagt cgtcactatg aaaaccgtca ttttagcaga
     3901 aaaaccgtca caagcgaagg cttatgccga tagcttttct aaagctaccc gaaaagatgg
     3961 gtattttgag atccaagatc gattgtttac aggagaaact gtgatcacgt atggttttgg
     4021 tcatttagtc gagttagatt cgcccgatat gtacgatgaa aaatggaaac aatgggcatt
     4081 agaacatttg cctattttcc ccaatcaata ccactatcat gttcctaaag ataagaaaaa
     4141 gcagtttagt gtcgtcaaac gacagctgca gtcagctgat acgattgtga tcgcgactga
     4201 tagcgatcgt gaaggggagc tgattgcctg gtcaatcatt caacaagcag gcgccaatca
     4261 aggaaaaacc tttaaaagac tttggatcaa ctccttagaa aaagaagcca tttatcaggg
     4321 attccaacag ttacgagacg cagaagagac ctatccaaaa tttgaagaag tacaagcacg
     4381 tcagatcgcc gattggttaa ttgggatgaa cggtagccca ctgtatagtt tactcttgca
     4441 gcaaaaaggc ataccaggaa gtttttcttt aggtagagtc caaaccccaa cactctatat
     4501 gatttatcaa ttgcaggaaa agattagaaa cttccaaaaa gaaccatatt ttgaaggaaa
     4561 agcccaagta atcgctcaaa atggcgcatt tgatgcgaag cttgatccaa atgaaaccca
     4621 agcgacacaa gaagcatttg aagcctattt aaaagaaaaa ggtgttcaat taggaaaaca
     4681 gccaggtacg atccttcaag tcgaaacaga gaaaaaaagt gcggctagcc cacgtctttt
     4741 ttctttatcg agccttcagt ctaaaatgaa tcaactcatg aaagctagtg ccaaagatac
     4801 tttggaagcc atgcaagggt tgtatgaagg aaaatatcta agctatccgc gaacggatac
     4861 cccttatatt acggaagggg aatatgcgta tctgttggat cacttagacg aatacaagca
     4921 ttttttaaaa gccgaagcga ttccgactcc tattcataca ccgaatagtc ggtatgtgaa
     4981 taataagaaa gtccaggaac attacgcgat cattcctaca aaaacggtta tgacagcagc
     5041 ggcttttgaa caactaagtc cactgcagca ggctatttat gaacaagtgt taaaaacaac
     5101 ggtggccatg tttgcggaaa agtataccta tgaagaaaca acgatcttga cgcaagttca
     5161 gcagcttcag ttaaaggcaa tcgaaaaggt gccgctagat ctaggttgga agaagttatt
     5221 tggaaaagag agcgacggaa aagagaagga agaagagccg ctacttccta aagtgacaaa
     5281 aggagaaatg gtgacggtgg atctacaagt tcttgaaaaa gaaaccaagc caccacaacc
     5341 ttataccgaa ggtacgttga tcactgcaat gaaaacagct gggaaaaccg tggatagtga
     5401 agaagcgcaa tccatattga aagaagtgga aggaatcggt acagaagcga cacgggcgaa
     5461 tattattgaa accttgaaac aaaaggaata tatcaaggta gagaaaaata agctcgtggt
     5521 gacgaataaa gggattttgc tttgccaagc cgtggaaaaa gagccgctgc ttacgagcgc
     5581 cgaaatgacc gccaaatggg aaagctactt gttaaagatc ggtgaacgaa aaggaacaca
     5641 aaccactttt ctagccaata tccaaaaatt tgtctctcac ttattggaag tcgtaccagg
     5701 gcaaatccaa tccactgatt ttggatcgac gttacaagaa gtgaaagcag ccagtgaaaa
     5761 gcaagaagcc gcgcgtcatt taggaatttg tccaaaatgc caggaacaag aagtcttact
     5821 ctatcaaaag gtggcagctt gtacgtctga agcctgtgat tttaaaaggt cagaagggtt
     5881 catttgaagc aacgattgtt ttaaaagaag acttcactac aggctttgaa ttttctgaaa
     5941 agaaaaaaac gaacttcaag aaaagaacac gccgcactgc aaaataatgg agggttaatc
     6001 ttatgcgtaa taccacacac aaacaacaag aaatcgaaaa tcaaaaagcc aaaattcgtt
     6061 cagaacagga aaaaatgcgt gccagcaaac gaagactgca gcaattgaag aaccagcaga
     6121 ggattcaaaa agaaatcgaa ttagatcgat aaaatcttcc taactattgt atacgtataa
     6181 acttatgtta tactgttttt ataagtttat acgtatacaa aggagggaga aaatgacaaa
     6241 agcaatggta tacaagctgc tagaagaatt taatggaact ttggctttga aggatgccaa
     6301 aaaagcgggc atttctcctg taacgatcaa acgtatggta gatcgagggg agctggatcg
     6361 agagtatcca ggttttttca ccttacctgg tcaatttcct gatgaattgt ttatggcgca
     6421 aaaaaaatat gaacgcggca ttatttcaca tattaccgcg ttagatttgt atgatttaac
     6481 agatatgatc cctagacaga tcgatctgac tgtaccttac ggttatcacg tttctgaaaa
     6541 aggattgaag gaattcgcag ttgagctaca ctatacaaaa gcggaatggt atgaattagg
     6601 taagattgaa ataaaaagtc gctatgggaa tcctattata gcttatgatc cagaaagaac
     6661 tttatgtgac atttggaatc cctggtatac cgttgaggat gaaatcaaag taaaagcaat
     6721 caaaaactat atggagtcgg acagaaaaaa tcttagaaaa ttgaatgagt atcgacgaat
     6781 tttaccaaca gacaaaacga tgcgttctta tattatggct ttaaattaaa ggagaagata
     6841 catgacacct acgcagttaa aagcaaaagt gaagaatatt tctagagaaa aagaagttga
     6901 tccgcagctt gttttaagac attttatgat ggaaaaattc ttggaaaaaa tctcagagtc
     6961 tccttatcgt gaaaattttg tgttaaaaga cggcttcttg attggctcaa agtatggcat
     7021 tgaaaatcgc acaaccaaag atattgatac gactttacgg gaaatgaaag taaccaaaga
     7081 aacattaaca acggttctaa acgaaatttt ttcaacgccg acgaaagaag gcattcaatt
     7141 tgagattcaa ggaatgaaag aaacgagaga agccgattac tatccaggat ttagcttacg
     7201 tgtgctggca catttggaaa acatgcggcc agattttaag gtagatgtga ctacaggtga
     7261 ctcgatctat cctgcaacga tcactcacag tcataaattg atgtttgagg atcgaacgat
     7321 tcctttggaa tcctatccta cagaacaaat tattgctgaa aaattaagtg caacctttga
     7381 ttttttaaca gataattcga gaggaaaaga tttttatgat ctctatacta ttcctaaaat
     7441 ggagaagatc gatgagaaga ccttgaaaga tagtgtgaga aatacattta cacgtcgagg
     7501 aaaaactgat ccgttaaaga tatactatga aaaggatatg gatgtactga aaaatagtca
     7561 cgagctaaaa gatacctgga agaaatatca aaaaagcaat ccttatgctg cgtcgatcac
     7621 gtacgatgag acaatggatt cgattagtaa tttgatggaa aaagtgattc acgtcgaaga
     7681 aaaagagcga aaactagctt atcaaagaca ccaacagatg aatagagaaa tggagagata
     7741 aagatgatta ataatgtgac attagtagga cgcttaacca aagatcctga tttacgttat
     7801 accgcaagtg gtactgcagt agcgactttt accctagccg tcaaccgaaa tttcacgaat
     7861 caaaacggca attgagaagc cgattttatt aactgcgtga tttggcgcaa accagcagaa
     7921 acaatggcga cattagctaa aaaaggtagc ttgattggtg tagttgggcg gattcaaacg
     7981 cggacttacg acaaccagca aggacaacgt gtatatgtga ctgaagttgt agcagataac
     8041 tttcaactat tagaaagtaa agcagcgaca gagagccgtg cgcacgctga tcaatcgtcc
     8101 accagtccaa gcaccgccac ctttgaacaa cggaatacag cgacgcctaa caacaatgga
     8161 ttgaacgctt cacagaatcc atttggtgga caatcaatcg atattagcga tgatgatcta
     8221 ccgttttagt ttgagaaaga aggagtaagc atggcaaaaa aaattagtct gacgatggat
     8281 ctattgatac ccgtaattgc atttatagac gggatttctt tgggcgtcac tgaaaatcct
     8341 ttggtaaata gaattgcgaa aggatcacta tttcttgtat tcgtgtatct tgtctgttct
     8401 tatcttttaa aaagagcagg aaaggataat tccttttggc gattgactaa agtcacagta
     8461 gcagcgacag ctgttatact tacggctatt ctaccgcttt atatgtagag ttaacgggaa
     8521 atcgttgaac ttcttgcgaa atattgctat aatgaacatg aaaaagagga gttgcgctaa
     8581 caacccctca ttaacagcac cgattaaaga acggtggcag aatattagtt atttggaaat
     8641 aaccgctgac ctgctaaagt agacggttat ttctttttgt catttttaag catatttaca
     8701 atcaaactaa tcaaagcaat ggtaaacata ccaaaaccaa gaatggtttg aattgtctca
     8761 tatgcggaca aaaggcttac tcctttcata gattttagca cttacattca taagcaccac
     8821 cacctttcca gaagtcgcca ccgtcttcaa actttactgt cttgactatt atagcatatc
     8881 taaaatcgaa taaaaattct caattttagc tgtttttgct ataattttag cgaggtgatc
     8941 gtgatggaac aatatattgg tttactcgat aaattagggt gatcaagaca cagccattgc
     9001 ttgtgcgatt tacattacgc acgagtcatg aaccaatcaa ttgtgtagtg gccaatatag
     9061 aaatagcgga taaattgctg attttggaag aaggcaagta tactatttca gtgacaggtc
     9121 acttcaataa acgaaagcaa ctagtcattg aatcaatgca aattagaaac cctgatcatt
     9181 ttacgcgatc gatgagcata taaaagaaga gctgcagtcc taaatgagga ttgtagctct
     9241 ttttgtttta catagaaaca gtaaaaatga aaaggcctgt ttaggaaggc acaagcgcct
     9301 tctcaattga aagacctttc tctcactcta accgccaccg ccgttcgaga agaaaggaag
     9361 tgaacgctac gcgttcacta agataaaggg tcttgcatag caagacaaaa tagaaaaaat
     9421 ttcctctcac caaaaggttc gctcctgttc gttatctctt tattgatttg tcagaccggc
     9481 aacaagttgc cttttatcac caaaaagcga caaggacaag ggtaaatggc acgctgcgcg
     9541 cccttgtctt gtcgcttttc gttgattcat tctggccaac aaatcaataa agaaagaact
     9601 aacgaaagga agtaaaacct ttcctaagag gaggcagaat caatcatgcg aaagaaattt
     9661 gattattggg gtgttccctt cagcgaatta ttcccaaact atcatgcacc acatactgtc
     9721 gaatgcgatt gtggagaaag agcgaaatgt atcaaaagct attgtttgta tcaatgtcca
     9781 acatgcggga aaaaatatac cttgtcttat ggcgattata ttttaattga tgagaaagga
     9841 aagaaacgct gatgaaaaca caacacaaat tattgactgt aaaagaagtc gttcaaattt
     9901 tggtggacaa agaagtcatt tctgaaaaag tagcagaagt tttacttatt ccgcggtact
     9961 ggacgataga aaccccttta gtagaggatg caccgactat gctatttgaa gacctagtga
    10021 agattgcagg atatgaactg cattatcaag aaaacttgga aacttatgaa aacgaatatt
    10081 ggttaaccaa attagagaac taaaaaggag aaaaaaatat ggctcaaaat tactatgacg
    10141 aatttgtcaa attacctttg gataaaatgg cacaaaaaat ggaggatatg acgttcctat
    10201 acaacgaaac acgtgttcca aaaaaacatt ataaagaaaa attgtcggta gctgtggaag
    10261 aaatgattga ataaggtgtc gaaatgaatc ttattgcgac gtactatcga acactggaag
    10321 aactgaaaaa gcaaaatgca aaatggtttt tccaagcctt actatgcttg gaagtaggtg
    10381 tcaaaccaag cactattaaa ccttctgaat accaagcttt agaactaacg tatgcgaaat
    10441 ttatcgaaac gaaaaaagcc aagactgttt caagtgaatg gttagattat tttgaaaata
    10501 tcaataagta tggcgcatat tacaccatga aaaaggaaga caacgaaaat gaataaggtg
    10561 atttatcaac taacaacgga ttccatatca acgatttgaa agaaaaaaag gtcaataatc
    10621 atttataccg aaaagtgaga atgtgtgaaa caaggacaaa aaaagcctag taactaagga
    10681 aatggttctt atttcgcacc aatcgctcca accgcccccg ccgcttaggt ggtgcgaaat
    10741 aaacgggcaa agcccgataa cttaagagaa agaaggtgat ccatttgaac aagattattc
    10801 ccttattgtt agtactgttt gttggcgaaa tacttatgcg agatagtgca gctgtacagt
    10861 taaacctcgc tgtatttttg gtaatactgg tgttttgttt gatcttttgg cggtttgtat
    10921 tagtcctcgt gagtctacta ttcaagatga ttgcagccat atttttcata gggttatttc
    10981 gatgaaagga gtgagtacca atgccaattg caataggaaa taaacggtta cccgtgacat
    11041 tagatgaaaa gagacaaaaa gaattgcagc aactaaagca gaagtatggc aaaagtgaat
    11101 ctaagattat gtgtattgcg ttagatctat tgatcgcgca agaaaaagca ggatttgatg
    11161 taccagcgct aaaaaagtga cgtcactttt taatttgatc tatataagtg atatcacttt
    11221 taccaaggag ggaattaaat gttaaaaact aaaaagaatt cgtacaaatt aaagcttagt
    11281 atactatcta ttatttgtct tttaggcatg ataattagtg gtcatttgta tagagtggca
    11341 tatacgcagt ttattgagta caaggttcac tctttaggat tagctgggga tcgatacgtt
    11401 gaatatgtcc attattcatt aaaatgttct tcatatattc ttctttggct tattttttca
    11461 ttgtttgaat tgctaaattt aatagcaata tcttcatatc acttagataa gctaaatacc
    11521 aaatctagcc gttataacga agtgtttaag gaaaatgtga agttagaaag tgacttaaac
    11581 aagctaaaaa aataaaaaga agaaaacata agttaactaa aattatttta ttaagaatga
    11641 tggaatataa ggataaggtt accaacataa cgatcattac ttctctgata tgctatggac
    11701 ttctatcaat cagtattttg tctatagatt atggagtaac tatactgcaa gccttagttt
    11761 tggctatgcc tatgtatcta tctttgttta tcatgcatga aatatataag ctatttgttt
    11821 tatcaaaaaa tgtagaaata cgggtaaata ttttggtgaa tagttatcaa aaagaactca
    11881 aagaaagaag acggtggcgg tatttattga aaggatgaaa aagtaggatt tgacatactg
    11941 gtattcagaa agtgccgtca cttttaaaac aatggaaaag gtggagataa gatgaagaaa
    12001 aaacaaacta aactatcatt agcagatatt taacacagtt aaccgcaact gaagatggtg
    12061 ttgttgaatt tgaacaatct gaaattgcgg tagtagatga tcgttacctt aaaatgcctt
    12121 acttctttga ccaagcaaag gtcatttgcc tttgtcacta cgatggggta cgtgatttta
    12181 ggcgttaaaa agtagtgatt atatcaactt tacgagtaaa tataaattta gacatgaaac
    12241 tgaattatcc aatggaaaat catggtttat aatctagaaa aattatagta atgattggat
    12301 tatatatatt ctttcgtatt ttcgtacaac tgtagcttgt caaaattgag aaatttaacc
    12361 gattaggtac tttacatcgt tatttgggta atttctaaaa catgaagctt accaac
//
LOCUS       DACDEG010000070        12359 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.074, whole genome shotgun sequence.
ACCESSION   DACDEG010000070 DACDEG010000000
VERSION     DACDEG010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.074"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(276..1445)
                     /locus_tag="IV142_001996"
     CDS             complement(276..1445)
                     /locus_tag="IV142_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP8485649.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            1649..2824
                     /gene="mltG"
                     /locus_tag="IV142_001997"
     CDS             1649..2824
                     /gene="mltG"
                     /locus_tag="IV142_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP8485650.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            2960..3439
                     /gene="greA"
                     /locus_tag="IV142_001998"
     CDS             2960..3439
                     /gene="greA"
                     /locus_tag="IV142_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP8485651.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            3608..4339
                     /locus_tag="IV142_001999"
     CDS             3608..4339
                     /locus_tag="IV142_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP8485652.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            4336..5403
                     /locus_tag="IV142_002000"
     CDS             4336..5403
                     /locus_tag="IV142_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8485653.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            5410..6042
                     /locus_tag="IV142_002001"
     CDS             5410..6042
                     /locus_tag="IV142_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8485654.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            6182..6847
                     /locus_tag="IV142_002002"
     CDS             6182..6847
                     /locus_tag="IV142_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP8485655.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            6875..7168
                     /locus_tag="IV142_002003"
     CDS             6875..7168
                     /locus_tag="IV142_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP8485656.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(7236..8183)
                     /locus_tag="IV142_002004"
     CDS             complement(7236..8183)
                     /locus_tag="IV142_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP8485657.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(8361..9164)
                     /locus_tag="IV142_002005"
     CDS             complement(8361..9164)
                     /locus_tag="IV142_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP8485658.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            9310..10224
                     /locus_tag="IV142_002006"
     CDS             9310..10224
                     /locus_tag="IV142_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP8485659.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10272..10742
                     /locus_tag="IV142_002007"
     CDS             10272..10742
                     /locus_tag="IV142_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP8485660.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            10827..11435
                     /locus_tag="IV142_002008"
     CDS             10827..11435
                     /locus_tag="IV142_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP8485661.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            11569..11769
                     /locus_tag="IV142_002009"
     CDS             11569..11769
                     /locus_tag="IV142_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8485662.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT         4156 a         1898 c         2487 g         3818 t
ORIGIN      
        1 catgaatgat ttgctagcac atattttagc aatctaaaga tactatttta gatttaatag
       61 gccctttgtc aaataatgtt ggtaaagaga aatcttagaa taatttggac agaacagaaa
      121 gaagaacacc tctttctgtt ctgtttattt ggaattaaag tctgtagcaa aaattgattt
      181 ttagatttgt cccacactct ttcctacgtt tagtcgagta ctttttttaa gcaaatgcta
      241 ttcaaagaag tagtatttgc tatcttttca tcatatcagc tatttgccaa cttagtagtt
      301 ccttccatca ggtcatagat ttggtttctt agatcagcag taggcagttt gatagttttg
      361 tcagctttta tattgctttc catgatgata gcatcctgtg aatctttgct aaatatagca
      421 gatgtatcaa aagagtcgat atttccttgc gacaggatat aattatctcc gctatatacc
      481 ccaccactat atggatgtcc attcgcattt tcacgccata atgagaagag taagtctttg
      541 gaaactagtt ttccagataa aacttgtgta aacagcgtat ttaaatcata gacggacata
      601 ctgatattac cagtccctaa ttcgttatgg atctctgttt cagattctgt cagtggttta
      661 aagtaatcat cctctttaga catttgatag gatagtgcat gattggatga atttttgact
      721 tggtcataaa attttgtatg ctgtaatcct aaaggttcga tgatatcatc ggtcaataat
      781 ttttcatacg gttgatttgt taatttgatc aaaattccag tcaatatggt aaagttcaat
      841 gaatcataat gagaggtctg ataatctgta aattttaagt gctgaatcgc aaattgaacg
      901 acttcttcat cagtcatcgg tatggttgga gaagcagttc ggtgcaagcc tgagcgcata
      961 tacagcaagt cacggatagt aatttgctgg ctgccagcaa tttgtggata gaattgagcc
     1021 agcttagtat ctaaagaaag gtgcccacgc tcgacttgtt ttaaaatcaa aagagtagtc
     1081 aatgcttttt gaaccgaacc aatctgataa taaagagaag gatcatttgg ttgttgcgtg
     1141 agtttattcg catagccata gcctttttcc atgatgatgc gattattttt ctttacgtaa
     1201 attgagccaa tataatcatt ttttcttaat agttgatcga ttttttcaac gagctgttgt
     1261 tcattggtag tcagtatcaa ctttttttga gctgattgag catgggcaac tatctgagag
     1321 tgttcattat tgtaaaattg ccatccaaga atagttcctg tagagaacgc actaaggcta
     1381 aacaacacga caacgatcat taaaaatgaa tattttcttt ttttagcatg ttttcctttt
     1441 ttcataatag tttatttagt ctcctttctt ttttacagaa agaataaagc taaaatagct
     1501 tgtatcttga taacatttat tttaatctta tagtaatgga tttactactt acatttttga
     1561 tagaatattt aaataaaatc attcacgcca attcaaatat catgtataat gaataaagtt
     1621 acgaacttga tagtaaggag gagttgtttt gtcaggaaac aaacaacacc caagtaaaga
     1681 taaaaaagaa gccagtaagg cagctacagc agatataatc gacaagcgga gaaatctgcg
     1741 taaaaaagaa gataagattg tgaggaagat cattttagtg attgctctta cattattgat
     1801 tattggcgga tttcttggat ttaccgtgta tcgttatgta gatagcgggt tgaagccttt
     1861 agataaatca gatgaccaat tagtccaagt cgagatcccg agtggctctt ctaacaagca
     1921 gattggtgag attttggaga aggacaatat tattaaaagc gggattgttt tcaattatta
     1981 tacaaaattt aaaaatttaa ctggctttca agctggctat tatcagttag cgcctaatat
     2041 gactcttgac gagattggca aacagttgca agaaggcggt acttctgagc caacaaaagt
     2101 tgcagatggg aaaattgcca ttccagaagg gtacgatatc gatcagattg ctgaacgggt
     2161 agcgaaggta actggtaaag acaagaaaga atttttagat ttagtgaatg atgagacttt
     2221 ctttaacgag ttgcaccaaa aattcccaga actgttggaa agtgcatcta aagctgagaa
     2281 cacaaaatac cgtttggaag gttacttatt cccagccaca tatgattatt acacgagtac
     2341 ttcgcttaag gatctagtga ttgaaatggt gaacaagacg aatacggtca tgcaaaacta
     2401 ttattcagct atcaagcaga aaaatttgac tgtacaagaa gttttaactc tagcttctct
     2461 agttgaaaaa gaaggcgtaa aagaaaatga tcgtaaaaac attgcacaag tcttctttaa
     2521 ccgaatcaaa gcaaatatgc cacttcagtc agatatctct gttttatacg cattaggtga
     2581 acacaaagaa ttagtgacgt atgaagatac agctgtagat tcaccatata atttgtatac
     2641 caataccggc tatggacctg gaccttttga caatccaagc gaagaggcaa tcaaggcagt
     2701 attagaacca gcagaaaatg actattatta tttcgtagca gatacgagca ctggaaatgt
     2761 ttatttcgct aaaacttatg aagaacatat ggaattagtg gaaaaatatg taaataattc
     2821 ttagaaaaac aggctctaga cattgatcat ttttagaaaa acatttacat ttgaagaaaa
     2881 gcgtggtaat cttttttgga ctatattcct caaaagatta ccttttgcgg tattttatcg
     2941 aaaaaaggag acgttacaca tggtagagaa agtttatcca atgactctag aaggtaaaga
     3001 aaaattagaa aaagaattag aagaactaaa aacagtcaaa cgcggagaaa tcattgaacg
     3061 aatcaaaatt gcccgcggtt tcggagatct ttccgaaaac tctgaatatg aatcagcgaa
     3121 agatgaacaa gcatttgttg aaggaagaat cacaacaatt gaaaatatga ttcgttttgc
     3181 tcaaatcatc gataacgacg gtgtagatgc agatgaggta tcaattggta aaacagttac
     3241 attcatcgaa ctaccagatg gagaagaaga agagtacacg attgtcggaa gcgctgaagc
     3301 agatccgttt tctggcaaaa tttccaatga ttctcctatt gcgcaagcat tgattggcaa
     3361 acgtaaaaat gatgaagtaa ctattgctac tccaggtggc gatatgttag ttaaaatcgt
     3421 caaagtagaa caagcgtagt ctgactgaaa gatctagaga gttcatctta ctgatgatac
     3481 actctagatc tttttctctt tgtgtatttt agatttcaac aaacgatctt acatctaaag
     3541 tcctatagcc aacagttaaa aaaataggta tgatgaaatg agaatgtgtc aggagggaga
     3601 aagagcgatg aatagttctt ggcgtttttt tgtggtagtt gaagcactac tgctgttatt
     3661 tgctgtttgg caaatcgtaa acaatactgg acttttgctt ttagtgatat tcggtatttt
     3721 caatatatat ctagctatgc gcaaacatcc tcgtactaaa tttcaaaatt ttcaattgat
     3781 attaggaagt ttggtcattt tttttagctt agtcaacagt cctgctttat ggatgatggc
     3841 tgtatttgcc gttttattta ttggtctgaa aggtgtagag atttcaggga tagatttaac
     3901 caaaaatgct ttttggcgaa agaaacagat catgatgatc caaacagagc aattaaaaac
     3961 acataataat gagcggaaaa gacagcaatt attcggtaac caacgtattg gaaacgacgt
     4021 ctatgaatgg gacgatatca acatcgccgt catttctggt gatactatca tagatttggg
     4081 caatacgctt ttgccaaaag atgataatat tgtgattgta agaaaaggga taggcagaac
     4141 acggatctta gtacctttgg gcgtagccat ccgattagag catgcgacat tagtaggaaa
     4201 tgtcttgttt gaagaagagc aattttcttt gaaaaatgaa caaatcaaaa tctacagcag
     4261 tgattatgat gaaaaccctc gtagattgaa gattattact aataccttga ttggagatgt
     4321 cgaggtgata cgaatatgat gggaaaaata tccagagcga tgctagctgt ttattcgggg
     4381 attgcggctt tccttattat tctattttca cttttcactt atttttatgc cagcaatcaa
     4441 agtcattggt ggggagaatt actgcgtgca cgtttattat atgttccgct tatcttccat
     4501 ttgttagcca tttccttagg tgtaggatta attgtctttc tattattatc acttattcaa
     4561 aagacaaaat acgggaaaat cgaagaaaag ctgcgtgcac tttcttctgg caattatgaa
     4621 tccaaattgt tgcttcttcc gatcccaagt gcatcggacg atttatacat caaagatatt
     4681 gataaggaaa tcactaagat aaaagaaaaa atgattgaag tatctagtga attacaaatt
     4741 gtaacgagcc gtccccaata tgtagatgga caaaccaaag aagaaatttt agaattagaa
     4801 agacatcgat tagcccgtga gctgcatgat tcagtttcgc aacaattgtt tgcagcaatg
     4861 atgatgatgt cggcattgac ggagcaagca gaaaaaagcg agacaccaga aatgtttcgc
     4921 aagcagttga aaatggtagc agagatcatc aacgcttccc agtctgagat gcgtgcgctg
     4981 cttcttcatc tacgtccagt caatttagaa gagaaaagtt taaagcaagg tatcgagcag
     5041 ttattgaagg aattgcaaaa taaaatccag atttcgctga aatgggatgt agaagatgta
     5101 aaactatcta gttccattga ggatcacttg ttccgaatcg ttcaagaatt gttatcaaat
     5161 acattaagac atgctaaagc caatgaatta gaggtatatt tgcacaaaat agacaataat
     5221 cttttgttac gtattattga cgacggaaca gggtttaata tgaatgaaac gaaaacggga
     5281 agttatggat tgaacaatat caaagaacga gtagctggta ttggtggaac agtaaaaatc
     5341 attagtttta aaggtcaagg tacaagtgta gaaatcaagg tccctttgat gaaggaggca
     5401 tagataagga tgataaaagt tttattagta gatgaccatg aaatggtgcg cttaggcgtc
     5461 tcttcttatt tatctattca agaagacatt gaagtaattg gagaagctga aaatggacga
     5521 caaggctatg agaaggcgat gacacttcga ccggacgtca ttttaatgga cttagtcatg
     5581 gaagaaatgg atggtatcga atcaacaaaa gcaatcttaa aagattggcc agaagctaaa
     5641 atcattatcg taaccagttt tattgatgac gaaaaagttt atcctgccat tgaagcaggt
     5701 gcagcagggt atttgttgaa gacctctacc gcacatgaaa ttgctgatgc tataagggca
     5761 acgcaacgtg gcgagcgagt attagaacca gaagtgacaa caaaaatgat ggaaaaaatg
     5821 agtcggcgaa atgagcctgt attacatgaa gaactaacga atcgggaaaa tgaaatttta
     5881 atgctgattt ctgaaggaaa aagtaatcag gaaatagcgg acgaattgtt tattacactg
     5941 aaaacagtaa aaacacatgt ttccaatata ttggctaagc tagaagtgga agaccgtaca
     6001 caagccgcta tctatgcatt caaacatggt ttggtgaaat aaaaacgatg ataaagagga
     6061 taatcaaaag ctgagaaaat aactattatt tttcttggct tttttacaat aatgatgctt
     6121 gcttttgttt ttgtaaaggc tttgttatac tgagaaaaga agatactaaa ggaggaaaac
     6181 ggtgaaacaa aattttgcaa ttgtaggtct tggacgattt ggcggaagta tctgccgaac
     6241 actgatcgaa gctggtcaag aagtactggc tattgatagt aatgaagatc gggtcaatga
     6301 atatatgaat atagccactc atgctgttgt ggcaaatgca caggatgaaa tgacacttcg
     6361 atcattaggc ataagaaatt tcgatcatgt aattgtcgct attggagaag atattcaagc
     6421 cagtatctta gttactctga tggtaaagga aatgggcgta ccaaatattt tagcaaaagc
     6481 ccaaaatgag tatcatgcca gagtacttga aaagatcgga gctgatcgcg tcgtccatcc
     6541 cgagcgagac atgggaataa ggattgcgca taatttagtg tcaaagaata ttttagatta
     6601 cctagagttg tcagataaat attctttagc agaaatccga gtctccaatc ctaaattttt
     6661 caacaaaaca ttagctgaat taaatttcag gcaacgtttt gacttaacag tagtcgccat
     6721 acgaagagta gatcaaacgg ttatcgcttc tcctgcagca gatgaatatg ttcgtgaaaa
     6781 tgataatcta ctggttattg gagagacagg cgatgtggat attctagacg ataagatgaa
     6841 tcaatagaaa gaaaagagaa agtaggttgt tgggatgaaa ttaacggtaa cgccaaaagc
     6901 acaaaagtgg tttgactcag aaattgatct gccagaaact tacggaatac gtttttttgg
     6961 aaaagtctac ggcaaaacag aggttcatga tggtttttcg attggaatgt cagttgaaca
     7021 gccagaacat cctgtaaaga aagtggaaat ggatgggaca ctttttttta tcgaggaagc
     7081 agatgaatgg ttctttaaag gatatgattt acttgttgat tatgatccca acctggagga
     7141 gccaacctat cactttaaaa aacaataaaa aaagagaggg tgacacaatt tttgtcacct
     7201 tctctttttt cttttgaaaa tattgttttc agctattaat atcctgcaga caattttact
     7261 tgattggttt gaagctcatg ggtggcaaag tagcttttca gattggttaa aaaaatagcc
     7321 attaatttct tttgaaaatc aggtgtcaaa cctgcaatgt gggaagtaat cagtacattg
     7381 tccattttcc aaagaggatg atcttgtgga agaggctctt cctcaaaaac gtctaatgct
     7441 gcaaaggaaa gttgctggtt atttaaagcc tgcaccagat cttgtgtctt aacagaagca
     7501 ccacggccaa cattgatgaa tacagatttt ggatcaaagt gttcaaacat atgctgatca
     7561 aagagcccag ttgtttcctc cgttagaggt aagatattga ctacgaaatc tacttcagga
     7621 agaacttcct ttagttgatt taaagaaaaa gtcttttcca ttccttctgc tggatgaccg
     7681 gatgtattga ttccatagca gttcatgtct aatgctttgc atttttttgc aatcattttt
     7741 ccaatatggc cagttccaat gattagtatt tttttccctt ggatagattg gtaatggacc
     7801 gatgaaccta accatttcat gtcactctgt gccttttgtg cttgaaaaag accacgagaa
     7861 tacccaagga tgattcctaa gacatgctcc gtaatcgatt cactgtggat gccactacca
     7921 tttgaaagta aaatatgttt ctttgaaaaa gtctcaaggg gtagctgatc aacaccagct
     7981 gagatagatt ggacccattt caaggtagaa gaggctaata gtttttcttg gtagtctgag
     8041 ttccagccca aactgatctc tacattttgt agatcctctg gttgtaattc tgttttaaag
     8101 atataaccag gcgctatagt atggatttct tcgataaatt catcacgaaa tggacgatct
     8161 gcataaatga ttctttctga catagttaat ttcccgcctt tctttactct aagtgtagcg
     8221 catacaagaa gaactttgaa gcgccttgct tgttttttga gaaagaaata tttttcaaat
     8281 aaaaaagggg ctgtgacaaa aaaatgtcac aacctctaaa agggtctatt gaagtagaaa
     8341 taacgagttg tctttatttc ttaattgatc gatatttttg cccatccttt gacagcttga
     8401 gcaaaaataa agattcctgc aaaagcaatg aaggcgctag tgtcaaaaga aggaagaaag
     8461 accatcaaga ttgttgcaat tatagtaggc aatgaaattg aatagatcaa tgtttttaaa
     8521 caatcaagaa aagtagcttt tgttagtcga aagcgtgcat agagaaaagc agcaatgttg
     8581 gtaaatagca aagtgatcag cagattcaga aaactaggat aaatagaaaa tataaatgtc
     8641 aggactttta tccaaagtgg tatagatgct tcacttaaaa aagaacggag ctgcttacct
     8701 gttaagtttt ttaaagattc attggtgtag ggaagttcta attggttatt gttcaacaaa
     8761 gcagaagtcg tgcctgtatt aggaagagct atgataagtt tgttttttaa catcccgaca
     8821 ctaacaaaat tccccatcaa atcagtagag atgtcctgtt ctgttcgttt tccctctgga
     8881 tcaaatgtga agataatgga gtttgtttgg taaatgaatc cttctttttc ttcggtttga
     8941 agctgcccgt tttggattgt aaaatcagga atgcgagtag caatcttttg cccatccaat
     9001 tgaatgttgt ttaagacttg aaaaacttga aaagagattg gcaaagccat aattatactc
     9061 aaaactaaaa gatagacaat ggctttccaa aatgggacat ttctcgcctc ctttaggtct
     9121 ttgaatcgta taaaggaact gataattaat tgttttgttg tcataagtac tcctcatcta
     9181 gtagtgtcat cacttgcaat tctagcgaag tatcaagtga aatacaagtg ctttaaggaa
     9241 acagctgaat gattggtgaa atatcaaaaa aatgttattc ttgtctaaag actatccaat
     9301 ataaataaaa tggaggaagg aatagtgcct acacagccac agagatcatc aaaaaaacta
     9361 tttggagtag tggttttgaa tgcggtgatc actttatcag aatttattgg agggattatg
     9421 tcagggagtt tgtctcttgt ttcagatgct ttccataacc ttagtgatac attggcaatt
     9481 attttttctt acggggcaca aaaaatggcc cgaaaagaag caaataaaaa acgtacgtat
     9541 ggctaccagc gtttagaaat attgagtgct ttcatcaact cattcatatt gatcatatta
     9601 tccttgtttt taaccgttga agcattcaag cggttcactt caccagaaaa gataaatagt
     9661 catttgatgc taacagtagc agttattgga ttgctcgcga atttgttctc agtattatta
     9721 ttacgacaag aagcagatga aagtttgaat ataaaatcta gttatttgca tttgttaagt
     9781 gatacactgt cttcggtaag tgtgattatc ggagcaattt tgatccgttt tttcgatatc
     9841 tactggatcg atccagttat tacactgatc atttcgatat atatattgat agaagcgata
     9901 atggtgatca aaaaagctgc cgctattttg atacaaagtg cacctgctat cgactatgaa
     9961 aaaatggaac aagaaattga agcaatcgat ggggtaaagg atgttcatca tgtccatatt
    10021 tggcagtata gcgaaaagat aattattttc gacggacata ttgattttga agatcagcta
    10081 ctaagcgaga ttgaaaagat atatcctcgt ataatttctt tattaaaatt aaaatatggt
    10141 attacacatg tgacgatcca agcagagaca aaggcaaagg atcaaaaaaa attaattttt
    10201 ttaaataaag atgaaagtta ttgaaatata gagtatgata aataagaatt tttaataagg
    10261 taggttggct catgaagcag tatcgacagt taaaaggata tttagaagca aaaggatatt
    10321 gggagatact agtctatctc ttttttggtg gtttggctac gattgtaaat tttgttagtt
    10381 ttgctctcgc acgccaatat tttgatttaa gtatggcctt atccaattct atttcgtggt
    10441 tttgttctgt attgtttgct tttgtaacga acaaactttg ggtattccat tcaaaatcac
    10501 cgaattttac acatgcactt attgaatgcg gtaagttctt cttctaccgt atcctttctt
    10561 acggattgga catgggggca atggtattgt tgatcaatgt tatgaacagc aatgagtatg
    10621 tggcgaaaat catcactcaa attattgtta tccttgcgaa ctatattttc agcaagttgt
    10681 ttatttttaa agaaactgag gtttttgaag aagaagtagg tcaaagtgaa aaagacgact
    10741 aggcataaca attgaagaat ttccttctct ttgttactct ttgtaatgta gacattatta
    10801 agaaaacatt gaggaggaat ttttttatga cttacacatt accagatcta ccttatgcat
    10861 atgacgcatt ggaaccttat attgacgaag aaacgatgca tctgcatcat gataagcatc
    10921 acaatactta tgtgacgaat ttaaattcag caattgagaa atacccagaa ttaggcgaaa
    10981 aaacaataga agaattatta tctgatatgg acgctattcc aacagatatc aagacagctg
    11041 tacgtaacaa tggtggcgga catgctaacc attcattttt ctgggaaatc atggcaccaa
    11101 atgctggtgg cgaacctaca ggagaaataa aagaagcgat taatgaagct tttggtgatt
    11161 tttcttcttt taaagaagaa ttcaaaaaag cagccgctgg acgatttggt tctggatggg
    11221 cttggcttgt aatggaaaat ggaaaattag ctattacctc tactgcaaat caagattctc
    11281 cattgatgga aggaaagaca ccaattctag gtttggacgt ttgggaacat gcttattatt
    11341 tgaaatataa aaatgtccga ccagattata ttgcagcatt ctggaatgtt attaattggg
    11401 atgaagtaaa caaacgtttc gaagcagcaa aataaccatt tcctccgtca tatcagtctt
    11461 aaaaataatt tggaaaataa aagagcgaaa gcttgctagc gttttctaaa ttgggtataa
    11521 tgttcttgca aggttacttg caagaacttt tttggaggat atacatttat ggaacacgga
    11581 acggttaaat ggttcaacaa tgaaaaagga tttgggttta ttacagtaga cggcggagat
    11641 gacgtatttg ttcatttctc agcaattcaa ggagacggct tcaagagcct agaagaaggt
    11701 caagaagtag aattcacgat tgttgaaggt gcacgcggac ctcaagctgc tgaagtgact
    11761 aaattataat aatttgttga ataaagacac gacttcttag agagtcgtgt ctttttgtct
    11821 atttatgaag tttttagatt ttttggctaa gtaaatgcat ttgttcactt gacatgtcct
    11881 aactaattat caagcaagga acattttctt cgttagtaat ggattttaaa gaaatttcag
    11941 tttttggatg ttgaataaaa gaccatttgt gaaaccacta gtatacatac tcattaccat
    12001 gtcaattcta ttactttaaa agaaaatcgt tttactttga agaggagcat atctatccat
    12061 taaaagatga tggtaatgca atcactagtt cgagtcattt taagagccat gcagtgagct
    12121 ggaaataaaa cgaatagaag atgggtttaa caaaaggacc agtcatagga agaaattgtc
    12181 gtgaaatcga atgataatct gaacggtagg caaagactgt taaaaagatg ggaaaggtat
    12241 gtgcgatcat tttactgcac gattatttgg aaaatacctt gttgttcgct ttttctggaa
    12301 aaaatacata aaaaaagata aaacgattgc gaactgcgtg tattcggtct ttcgatctt
//
LOCUS       DACDEG010000071        12245 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.075, whole genome shotgun sequence.
ACCESSION   DACDEG010000071 DACDEG010000000
VERSION     DACDEG010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.075"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..358)
                     /locus_tag="IV142_002010"
     CDS             complement(<1..358)
                     /locus_tag="IV142_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8485663.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVSQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVR"
     gene            992..1963
                     /locus_tag="IV142_002011"
     CDS             992..1963
                     /locus_tag="IV142_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP8485664.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     GSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            complement(2041..2277)
                     /locus_tag="IV142_002012"
     CDS             complement(2041..2277)
                     /locus_tag="IV142_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8485665.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(2246..2377)
                     /locus_tag="IV142_002013"
     CDS             complement(2246..2377)
                     /locus_tag="IV142_002013"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8485666.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            complement(2453..2836)
                     /locus_tag="IV142_002014"
     CDS             complement(2453..2836)
                     /locus_tag="IV142_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP8485667.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            complement(2869..3654)
                     /locus_tag="IV142_002015"
     CDS             complement(2869..3654)
                     /locus_tag="IV142_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAP8485668.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            3832..4932
                     /locus_tag="IV142_002016"
     CDS             3832..4932
                     /locus_tag="IV142_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8485669.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            4922..5746
                     /locus_tag="IV142_002017"
     CDS             4922..5746
                     /locus_tag="IV142_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAP8485670.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            5752..6558
                     /locus_tag="IV142_002018"
     CDS             5752..6558
                     /locus_tag="IV142_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8485671.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            6555..7385
                     /locus_tag="IV142_002019"
     CDS             6555..7385
                     /locus_tag="IV142_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8485672.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            7392..8423
                     /locus_tag="IV142_002020"
     CDS             7392..8423
                     /locus_tag="IV142_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8485673.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(8530..9803)
                     /locus_tag="IV142_002021"
                     /pseudo
     CDS             complement(8530..9803)
                     /locus_tag="IV142_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            10080..10289
                     /locus_tag="IV142_002022"
     CDS             10080..10289
                     /locus_tag="IV142_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485674.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            10339..11055
                     /locus_tag="IV142_002023"
     CDS             10339..11055
                     /locus_tag="IV142_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAP8485675.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(10957..11477)
                     /locus_tag="IV142_002024"
                     /pseudo
     CDS             complement(10957..11477)
                     /locus_tag="IV142_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            11765..>12245
                     /locus_tag="IV142_002025"
     CDS             11765..>12245
                     /locus_tag="IV142_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8485676.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     "
BASE COUNT         3524 a         2273 c         2784 g         3664 t
ORIGIN      
        1 ggcgaacata attctttcca aaagttgaga aatgggggat tttctcctcg aaggagtagc
       61 ctaaaaacca gcggtaagcg acattggtat cgacttcctt aatggtttgt cgcatggaac
      121 ggataccgaa taaatactga ataaacacca atttgatcag aatcactgga tcgatacttg
      181 ggcgacctaa atcggagtaa gtggcttcga cgattggata aataaattca aaatcaagcg
      241 cggcatcaat ctttcgaacg agatgatctt gagaaactag atcatcaatc gtaagcatcg
      301 caaattgatt tctaccattc gtattgtttt tagacatcat agctatcact ccttgcattc
      361 gttttcataa atctattata aaagaaaaga ctgtagacat catccataaa atggactttg
      421 tctacagtct ggataacgtg attttttgct gtaatgacaa ggttttagct gtttcagata
      481 ccatgtatcc aaaaatagtt tattgagaaa ataggagagt atttgatact aactagtagg
      541 aaaaaatgag ataaaaagta aatggaatca tttaaggtaa tctgaaataa aaaatataca
      601 gccctgtggt actttaccta caaggctgta tatatttttt actctcagcg tttttgactc
      661 cgtgtctgac tccgttttat tgattttgaa tgttattcaa tgaccattta acaggtcaaa
      721 tttgatgttt ttgattccgt atgacgccgt ttgatatcta gtgatatagt gatttgagtc
      781 tacatcgaat aagggaataa accaataaaa aatgatttag aatattaaaa taacaaaaaa
      841 gaggactacc tgtattagtc ctcttttttg accagatttt gaccagtcta ttttacatcc
      901 tagtatattt atatgagcta attttatgat taaatggtaa gtttttcctt gtgtgtttta
      961 gtttaaacta gtgtgtaatt caaaaatcta tatggaagga acagtattaa gaatagaaca
     1021 gggttctcta catgatggtg ctggtttacg gaccgttgtt tatttaaagg gctgcccatt
     1081 acgctgtgcc tggtgttcga ttcccgaatc acaaagcaag cagattgaaa aaggatttgg
     1141 ccagaccatg accgctgaag aggtaatgga tgagattgag aaggatgctg ttttttactt
     1201 tcactcggat ggcggagtga ccattagcgg aggcgaggca ctggttcagg cggattttgc
     1261 taaagaaatt ttgcaaaaat cgaaatacat tggaatcaat accgtactgg aaaccagctt
     1321 ttgtggtgct tataatgaaa tccaaaaggt agctccctat gtggatacat tgtttgtgga
     1381 tgtaaaaatg tttaccagca agctccacaa acaatggacc ggcttagata accaacaaat
     1441 tttgaagaat attcgtcgct ttttaattga gtatcctaac tgcgaagtgc gaattcgagt
     1501 tcctgtggtt ccaggtatca atatgaacct gaccgagctg ctgacgattg cttgttttgt
     1561 ggcagatctt gatcggttcg taccgttaga attactaccg taccactgct atggtatgca
     1621 cggttaccaa gcattgggac tggagtatcc gttggcagac acgccagcgc cttcacctaa
     1681 agaaatgttt gcattggcag atcaactggc tcgcacggtt cctcatttgc tggtcacagc
     1741 tttaaccaaa acatttattt atttattgaa tccaagcttt atcttatgga taaatgaaag
     1801 acactcacta gctgcagccg gctcaagaat agagcttttg ctgaagtcag tgagtgtctt
     1861 tttacgatgt tttttcgaag ttaatttgtc gaatgaacac tggctcaatc ggtacatctt
     1921 cactgtttgt cggctgagct gcgatcgctg ctacgatttc taacccttgg tatacctgtc
     1981 caaaaacagt ataacgacca tccagctccg gtagtcctcc aagctccaag tatgcttgct
     2041 ttacattagc aggtcagcct gcgcctttgt gttttgcaca ataaaaaact ggttgccgtt
     2101 ggtgtggggg ccgtggtccc ctaaggccaa ggcgccatta aaatgataaa gtccgcgatg
     2161 cacttcatca gcgaaaaagc cctcatagct ggaaacagct tcatcaccag aaccatccaa
     2221 agcgccacct tgaataacaa attccttaat gacacgagcc gaaaggtgtg ttgtcataaa
     2281 cgccttgctc agctagggag cgccagttgg ctacagccag gggagcttgg tcaggaaata
     2341 agcggagacg aatttcacca gcatcggtga ccagcacaca aacttcttcc tgctgcggtt
     2401 tttctaattg aaagagagga aggtttgcaa ctgaaacgcc ttgatcctgc catcaaatat
     2461 cgaaaatccg ccactcgacc ttcgcctgat caccaaggcg agccattccc aataaaacgg
     2521 tataggcggt ttcatccgct tgaaaggtca attgcagctg tcctcgtaaa tcgttgtagt
     2581 catctggggc ggctacttga taaaaaagat gaaactgcgc atgcaaatat tccaaaggct
     2641 gatctttaaa ggctgcaaaa taagcctgaa ctttttcccg ctgcaagctg tcagccacca
     2701 aaaaattttc cagttgttgt tttggaacgg ctgctaacaa ggatcggcaa accgcctcaa
     2761 ttttcaacgc atcccaatgt cgcgcaatta aataatggat gggtgcctct tgattgtagt
     2821 cccacaggtt gctcattttt ttcaccactt tcttctatta tataggaatc acaaaatcgg
     2881 cagcggcgtc aggcggcgtt cttttccttg aatgactagc aacttttcta cctgacagtc
     2941 tgccaacagc tgactcattt ttttaaagcc cagctgataa tcttcggcaa catgagcatc
     3001 cgagcctaag gtaaagagct ttccgccgac actttgatac aaaggaacgg cataacggta
     3061 aaggtcttcg ttgccgtatt tcaagaagct cttggcattt aactccatcg ccaattctcg
     3121 ttcaacaacc tttttgaaaa tcgttagcag ctgagcctca aaatgctccg cgaattcttc
     3181 cgctgtgaag gtgaagcggc gtaatccata atcaaagtga gtcaaaatat ctccttctgg
     3241 aaaatccgtc aagacctgca acatctgatc aaaatattcc ttggtagttt gaatgggatc
     3301 tttggttaaa accacatcgt ccatataatc aaagcggcca ttttgatgaa tacttaccag
     3361 ctttaaatca taaggatgct gagcgaggta atccacgatc cgctcttctt gtcctggaac
     3421 aacgccaatc tcgatacctc gcagaaattt ggtggaatag gtttctttta aagagtccag
     3481 tttgtttata taggctggat aatccggaat gtcatctttt ccggtagatg gattgtttaa
     3541 atcaaagtga tccgtagaaa cgaaaaactc cggctgatag gctaaatagt tttcaaattt
     3601 ttcctctgaa tcaaaggaaa aatacgtgtg gagatgctga tcataatact gcatagggag
     3661 cctccttttt tctactatca taccgcaaaa gctttagaag gttaacggga attcgtcgaa
     3721 atttacaaac catatacaac ctttacattg ttcatgaaac cttttcaaga aactgattct
     3781 ctcattacaa agctttgtta gaatggaatt atcgaaaggg gagaagaatg aatgaaaatg
     3841 aatgaaaaaa ttcgtaacat gactatcggt ttattttgtg cagcaacagt aagtatcctg
     3901 ctgacaggct gcggctcagg tgaagcaacg aagcaatcgg ctgcggcaga cgactcttta
     3961 gatacgatta tagaaaaagc caaggaagaa ggagagattg ccagcttggg aatgccggat
     4021 acttgggcaa attgggtagg cacttggaac gaccttgaaa aagaatacgg cttaagtcac
     4081 gtggatacgg atatgtccag tgcagaagaa ttggctaagt ttgaatcaga aggaaaaaat
     4141 ggaaccgccg atattggtga tgtcggtatc agcttcggtc cgttggctga aagcaaagag
     4201 ttaaccttac cttataagac caactattgg gaggaaattc cggattgggc aaaggatgat
     4261 gatggggatt ggttgttaag ctatacggga accatttcct ttatcaccga taaagagaat
     4321 gtgaagcaag caccgactag ctggaaggag ctgcaagagg gtgactatca agttagcgtg
     4381 ggggatgtga cgattgccaa ccaagcacaa tttgctgtat tggctgcggc gattgccaac
     4441 ggaggtgacg aaaaggatat tcagccaggt ctgaattact acaaaaaaat ggcagaaaaa
     4501 ggccgtttgt ccaccgttga tgccagcgta gcaaatcttg aaaaaggtga aatcgatgtg
     4561 gcagttgttt gggacttcaa tggactaaat tatcgtgacc aaattgactc cgagcgtttt
     4621 gaggtaactg tgccaacaga cggctccatc attagcgggt atacaacctt aatcaataaa
     4681 aatgcccctc atcccaatgc ggcaaagcta gcacgggaat atattttatc tgatgaaggg
     4741 caaatcaatt tggcaaaggg ctatgcgcga cctattcgca aggtagagct gcctaaagaa
     4801 gtagctgaaa aattactgcc ggaggatgct tatgaagcgg cggtgccagt tgaagacaat
     4861 gaagcttgga acaagacctc tgtagagctt ccagagcttt ggcaggcgga ggtactgacc
     4921 tatgcaaagt agacttgaca aactgattgt agttatacta gacggctgtc gctacgacac
     4981 agccgtcgcc cagcttgggt tcatgaatca tttagttgag caccagcaag caagcttatt
     5041 gaaggtaaaa agtgagctgc ccagcaattc acgtccgtta tacgaagtac tgatgaccgg
     5101 cgttccagcc tatgaaaatg gcatctatag taattaccat gctcagcgat ccaaagagtg
     5161 gtcgctgttt gaattagtta aggaagctgg cggaaaaaca ggagccgccg cttaccactg
     5221 gtacagcgag ttgtacaatc aagcccccta taatcgtttg gttgatcgca tccagcatca
     5281 ggaggagcga ctgattcagc atggcatctt ttatagtgag gacacttacc ctgattcgca
     5341 tttatttgcg gatgcccatt atttagtgaa tcagtatcag ccagactttt tggtcgtaca
     5401 ttcgatgaat attgatgatg tcggtcacaa atttaccgcc gattctcatg aatatgcagc
     5461 agcggttaat caggcggaca gattattggc tatttacctt cctgaatggc tgcaaaaagg
     5521 ttatcaagtc gtggtgactg cggatcacgg gatggatgcg cacggactgc atggcggcag
     5581 cttggctgca catcgtgaag ttccttttta tttgatttca aataaacgcc aaaagctgaa
     5641 ggaacaaact gtaccacagc tgctggcggc tccattgttt tgctggttat taggaattgc
     5701 accgtcagag aaaatgcagg acattcaaga attgatgagg ggatgaaaga gatgaaaaaa
     5761 cgcttggttc tggttcctgc ctcctttatc ttctgtgcat ttttactgct gccgctgctt
     5821 tttatggttc tgtgcagctt gcacagtgat ttaactaacc agtggacatt ggccaattac
     5881 agtgatattt tcagcaacaa atattacaca caggcctttg ttaacagcgt actgattgct
     5941 gtcagctcca gtgtgattgg gttaatcggg acgtttattt tatgtttatg cttaatgaat
     6001 ctatcgacta aattgcagga gaaaatcacc tttgtttcca atttggcagc taattttgct
     6061 gggattccgc tggcattctc ttttattatt ttgttgggga atgtaggtgt gctgaaaatg
     6121 attttgccat ttctagcgga cttcaatttg tattcttggt ggggattgac catcacgtat
     6181 acctatttcc aaattccgtt aggggtcttg tttttgtatc ccagcatgaa ggaagtgaaa
     6241 aaggaatggc tggagtccgc acaattgctg ggcgcttcat cagcgtatgc ttggttcaag
     6301 gtggctttgc cttttttgcg accatcgatt gccagcacct ttgtcatctt atttgctaat
     6361 ggcatgggga cctatgaaac agcgtatgcc ctgaccagca gtaatgtaaa tcttttaacg
     6421 attcgaattg cggctttagt gtcgggagat gtgttcgcca agcctaattt aggcagtgcg
     6481 ttggcagtcg ctttaggtgt cctgctggta acggcaatgt tgattattca aaagaaaggc
     6541 aaggtggtgc agacgtgaaa aagttaataa aaccgatttt gttctgcttt gtcctgtatt
     6601 acttgctgcc gattattgga acgattttgt atgccagctc cactaaatgg tcaaaatcgc
     6661 ttttacctag tgattttacc ttgcaatggt tccaacagct gctgacggac agggaattta
     6721 ttgctgcggt gggtcgctca cttttattag caggtgtggt cttggtgacg attctgttgt
     6781 tgatgattcc caccattatt tggattcatt tgtatttccc tcgtttgaat cgttggctgg
     6841 aaaagctgct cctgctgccg tatgctttac caggagtgat tttggtaacg gctttgcttc
     6901 gaacgtatgc tgaaactggt atcccaatgt ttgtagtgtt ggtaggtgca ttgtttatta
     6961 cggcgttgcc gattgtctat cttagtctta acaaccagat gcgcttaatt aatctgaagg
     7021 aattagtgga tgcagcagaa accttgggcg ccccgatgtc tacgattatt atccaggtgc
     7081 tgtttccaaa catccgtatc ggtgtgaccc tagtttctct gatgattttt tcttcggttt
     7141 ttggggagta catgctgacg aacctgttga ttggcggtcg atttcagacg gtacgcatct
     7201 atatgctgcg gcggatgaac gaaaacggcc atttggctag cgctgtgatg gttttgtatt
     7261 tgctgtttat ggtgggaatt gctttaacta cctttatgct gacttatcga caaaagcaac
     7321 agctgtccct taagcagacg agggaagaga agcagtcgaa aaagaagccc ttaaaggagt
     7381 tgtgacggaa aatgttttta gaaatacatg atctcaatct gttttttgat gaaaaacaga
     7441 ttttagctat tgatgaaatt cagattgaaa agggtgagct ggttacttta ctaggtccca
     7501 gcgggtgcgg aaagtctacg ttgctgcgct gtatcacggg attggaacaa ccaaagagcg
     7561 gtgagattgt gctggacaat gagaatattg ccgataagcc tacgaaggat cgcaatatcg
     7621 gctttgtttt tcaacagtat gcattgtttc caactatgac tgtttttgaa aatgtagcct
     7681 ttggtttgaa ggtaaagaag ctgagtcctg aggtgattca ggaaaaggtt tttgagatgc
     7741 tatcattggt ggatatgacg gagcaggcag ataaaaatgt ccagtttctt tctggcggtc
     7801 aacgacaacg ggtggcactg gctcgctcat tggtcactga accgaaagtc ctgctgctgg
     7861 atgagccatt aagtgctttg gatgcccgaa ttagaaaaca gctgcagcgg gatttgcgcg
     7921 ctatccagca atcgctaggg atgacgatga tttttgtgac tcatgaccaa gaagaggcga
     7981 tgcgaatcag tgatcggatt tttgtgatgg aagctgggcg ggtggctcag gtttccacct
     8041 cgaaggaatt gtatcagaat cctcagagcc gatttgttgc ggaatttatc ggcaactata
     8101 atcgtttcga atggtatgag ctgaatcagt atacccacga gttttccgct aagggcaatc
     8161 gctgcattta ttatttaaga ccggagctga ttcaatttga accggttcct cagggggtta
     8221 agattccggt tctatggaag gaatcattta ttttaggcaa cattcaacgg tatgtttttc
     8281 aaacgctgga gggcaaagaa attttagtgg atcggttgaa cgatcgggaa gagaaaatta
     8341 gcgatgcctt atacattcag ctgaaagata ttctagcaat ccctgtggaa attgatactc
     8401 agcattttgc acaacaaagc tagtgtatag tcggcaaaca tttctggctc tttgtcaaat
     8461 aggactgata gagtgatata agaacatcta aggtagaatt gattttctgc tttagatgtt
     8521 tttttatgtt taataagtga tcagatgatt gattaagaaa attctggttc tcatcctaac
     8581 ccgtaagcta ttcgtttcaa tgctttgatg tgggtatgca gattttctat tttgccattt
     8641 gagtaaggca actctaaggc taaacgaatg ccggattcat agtttataaa cacttttttt
     8701 atatgatgca gttctcttgg aaccgacttg ggcattgttt ttacaagctg aaagaagatg
     8761 tccgcttctt tgtcataaaa agcttgaaga atttcttgat agtaagcata agctattcgt
     8821 agttcatcag aagtagaaag tagacgttct acgacttcac tttcggttaa ataggcccaa
     8881 ccaaaattac gccattttgt ccatttatca taaacaagcg tgtcctgtcg tttgatcaga
     8941 agtcgagata aagccttcac ttgtcgatag tgtttggctt gtattggatc gtttcggtcg
     9001 aagcttttca tgactcatag tcaaatatcc ttaaaactgc gaatgaggtg cttgacgatg
     9061 tgaaagcgat ccacaacgag tttggcatga ggaaagcact cttttatcag caaagcataa
     9121 gaggcgttca tatctgttac gagtattttt acgtttaaac gtgcacgtcg ttcaaattgc
     9181 ataaaataag aaatgagatc tttcttctta cgtgaaggaa gaatatccag taatttccca
     9241 gtatcgccat ccatacaaga aaaagaatag ggaccgactt gatttttcaa tgaacgaaat
     9301 tcatctacca atgaacagcg aggcaaccaa tttattttca cgtttgtcat ggggagcaga
     9361 gaagcgagtg ttcgcgaaac ggttgaccaa gaaacgccgc aatgtttaga aatcgtcgtc
     9421 attgcttgtt tttcagtcaa ttcctccaat attttataat gtacttgttt ggcgataaaa
     9481 cagttttttt caacaatgta ggtattcgcc gtaaaatagg tgtgacagcc acgacagagg
     9541 taccgttgct ttgccaaagc aagataagtc cgttgattcg cacacttatt gagaaggacc
     9601 agcgattttt tctttccgtt cttaacgatc agcccatgtt gattcacctg tttacactga
     9661 ggacagggct gaatcgtttt aatgagcgtg gcagaaacaa cgatactgtt gattgaatgt
     9721 atttttttct tctcgacatg agtgatttga atgtttccat ctttgattcc tagtaatagt
     9781 ttgataagat gaagatcagg catacagaac gctcctttga ttgtgttgtg gtgattcaat
     9841 tctaacggag cttctgtatg ttttgttttt ttatgctcaa aaggactgat agaatgattt
     9901 actatcagtc ctagaaatta tagcgccaca tttctttcac ctttgacttt tcagacggca
     9961 gtttttatgc tgatactgta gagagagcag gacacagctt ttgcttgttt tgggtaaggg
    10021 ggaaagaaga tgaccaaagc acttattttt gattttgatg ggacgatgat tgacagtgaa
    10081 tggatgtttt acgagagtat tcatcactat ttactgaagc ctatttactg gagcagttcg
    10141 gctataaaat tccttttgca gactatcaac aaggtgttgg gacggcagca gaactgtcct
    10201 tttatgaagg gataagtcaa gcggttggga gaccagtgga tgctgaacat tttgagcagc
    10261 ggatcagcag cactggaaaa tcggagtagt ctcaattctg aactgcggga gctagactat
    10321 ttttttgaag cacatccgat gattaaggaa aaaattgatt cctttgtgac gatcgatgat
    10381 cttcaggtcg gcaaaccaga tccagaaggt tatttgttgt gcttgaaacg gctgagggtt
    10441 gaacaggctg ttgtagttga ggatagtcca gtcggtgccc aagcagcacc gcatatgaca
    10501 gttgatgtac aagcgttgga tgcagatttt ttcgccttta gcgggcataa aatgtgcggg
    10561 ccgacaggaa tcggtgtatt atatggcaaa cgtcaattgt tagaacaaat ggagccagtc
    10621 gaattcggcg gtgaaatgat agattttgtc aatctttacg atagttcatg gaaagagctg
    10681 ccatggaaat ttgaagctgg tactccgaac attgcaggag caatcgatta cttgaatgag
    10741 attggtatgg agaatattca tcgttatgag caagaactgg ttgcctacgt tttacctaaa
    10801 ttacaagcga tcggcggtat caccatttac ggaccgcaag atcctgaaca tcatactggc
    10861 gtggttgctt ttaatttgga tggcatccat ccccgtgatg tggcgactgc tctggatatg
    10921 gaaggtgtcg ctgtacgttc aggacaccat tgtgcacaac ggttcatcga cgatttctgt
    10981 ttgactaatt ggtcgtttaa acagtcgatg ataatgccgt ttgagcggct ccaatgtcgt
    11041 ggaatctttg agtaagagtt tccaatatcg cttgagccga cggtacttct tctgattttc
    11101 agattacgtg attgattttc gtacagttct atgagaatcg cctgcttcag ttcctttgtt
    11161 agttgatgat gttcatcaac taagtccgtt ttcgcagaaa aggtctgccc acaatcatag
    11221 catagaaaac gggtacgttt taattctaaa atagtcaact tttctttgac tttcaacatc
    11281 tgagtacgtg tcgtatagga accgttgcgg ataatacgaa tcgatccaca gtgttcacaa
    11341 tgggaaggtg agtcatccaa tctcccttta ataaactgcg cttgaacgcc tttatattct
    11401 ttttcaatca gccattcttc atcaaaaaat aaatgtttat ctgttaatcc gagtaatttt
    11461 ctagtatgat tatccataga gcgactcctt tggtagggtg atgttttggt cgactaaatt
    11521 ttaccaaaag tgagtctctt tttgagttga aacgaagaaa aagtgttagt agaaaatcgc
    11581 cttcgatttt ccaacaacac aaaatattat agaagccttt tttctaaagg atttcaaggt
    11641 gtctgtttgc atttattaaa aaaaagaaaa agtattggca acagaaggcg tcaccaacac
    11701 tagatattat agagccataa tatgctgtat aatatctagt gtataggctt aagtggaggt
    11761 atatatgaaa atattaactg ttgaagatga taatatgata agagagggaa taagtgaata
    11821 tctttcagaa tttggatata ctgttattca agctaaagat ggaagagaag ccttgtcaaa
    11881 atttaatagc gatataaatt tggttatctt agacattcag atacctttta taaatggttt
    11941 agaagtgttg aaagaaatta gaaagaaaag caatttacca attctaatct tgactgcatt
    12001 tagtgatgaa gaatataaaa ttgatgcatt tactaattta gtagacggat atgtagaaaa
    12061 gccattttca ttgccggtct taaaagctag aatagattct ttaattaaga agaattatgg
    12121 acatttagag aagtttgaat ataaggatct atcggttaat tttaatagct atacagctaa
    12181 aataaatggt gaagaaatag atgtaaatgc aaaagaacta gaagtattaa aatgtttatt
    12241 ggata
//
LOCUS       DACDEG010000072        11730 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.076, whole genome shotgun sequence.
ACCESSION   DACDEG010000072 DACDEG010000000
VERSION     DACDEG010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.076"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            691..1164
                     /locus_tag="IV142_002026"
     CDS             691..1164
                     /locus_tag="IV142_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP8485677.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1173..1400
                     /locus_tag="IV142_002027"
     CDS             1173..1400
                     /locus_tag="IV142_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8485678.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2036..2407
                     /locus_tag="IV142_002028"
     CDS             2036..2407
                     /locus_tag="IV142_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485679.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2663..2905)
                     /locus_tag="IV142_002029"
     CDS             complement(2663..2905)
                     /locus_tag="IV142_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8485680.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2937..3839)
                     /locus_tag="IV142_002030"
     CDS             complement(2937..3839)
                     /locus_tag="IV142_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8485681.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3852..4040)
                     /locus_tag="IV142_002031"
     CDS             complement(3852..4040)
                     /locus_tag="IV142_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP8485682.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4054..4617)
                     /gene="amaP"
                     /locus_tag="IV142_002032"
     CDS             complement(4054..4617)
                     /gene="amaP"
                     /locus_tag="IV142_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP8485683.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4655..5548)
                     /locus_tag="IV142_002033"
     CDS             complement(4655..5548)
                     /locus_tag="IV142_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8485684.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5626..6564)
                     /locus_tag="IV142_002034"
     CDS             complement(5626..6564)
                     /locus_tag="IV142_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8485685.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6598..6948)
                     /locus_tag="IV142_002035"
     CDS             complement(6598..6948)
                     /locus_tag="IV142_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8485686.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(6981..7883)
                     /locus_tag="IV142_002036"
     CDS             complement(6981..7883)
                     /locus_tag="IV142_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP8485687.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7876..8733)
                     /locus_tag="IV142_002037"
     CDS             complement(7876..8733)
                     /locus_tag="IV142_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP8485688.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9067..9876
                     /locus_tag="IV142_002038"
     CDS             9067..9876
                     /locus_tag="IV142_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8485689.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            9916..10413
                     /locus_tag="IV142_002039"
     CDS             9916..10413
                     /locus_tag="IV142_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8485690.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(10798..10871)
                     /locus_tag="IV142_002040"
     tRNA            complement(10798..10871)
                     /locus_tag="IV142_002040"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10835..10837),aa:Ile,seq:cat)
     gene            11058..11381
                     /locus_tag="IV142_002041"
     CDS             11058..11381
                     /locus_tag="IV142_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485691.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT         3403 a         2183 c         1967 g         4177 t
ORIGIN      
        1 ctattctttt tcatcttcgt atttttgtta caaaattatc gaaaaaaata aacatgtgcc
       61 atctatctgt tggcacatgt tttaggattt atggtacttt ttattcggca atccatttta
      121 tttttctact tcttcttaat acctctaaca cttttgattt ataaactaat ccaatgcatc
      181 tacttgcgct tattaagcta tttcagctag atagtaccac cccatgcgtc aatgcttgat
      241 tttccttttg tataacattg tataagcaat aaaggacaaa taagtggtga ttacaccaaa
      301 ccattgaata agaaaaattc tgatacgtaa ataaacatga acttgaaaaa attaatgaat
      361 cggttagcta ctacgtagtt gtcttttttc tcatacagtt ctgttaactt acacttctct
      421 tcgctagata ataaaaaatc ctccaactgt attgtccaat ataatcccag gcatctaaac
      481 tagcattata gaagagacac acatagaatc taaaataact ggttttaaat gtttcttaca
      541 atcaaaaaaa gctgttaaat gcactccaca ccttcatact ctatcatttt ttataaacga
      601 tatattttta ttgtttttcg ataaaaaata gttgttttac aataattatt ctgctatatt
      661 ttagctatca aagataaaag agggataaaa atgaaattta aaacattgct attaaaattt
      721 gtcgttttag gtataagtgc cgtatttatt ttgttcggcg tactttttta tactcagatc
      781 gctacaagcg aaaaaggatt caattttgat tggcagacca cattatttgt ttctgtcatt
      841 tactttgccc ttctcctagg gatcgttatt gcttatttct tattgaaact attgaaaaaa
      901 atcgaagata accaagcttt ttccactcaa agcttaaaca tactaaaaaa aatcgaacga
      961 accattttgt ttattggttt cgcctttttt ggcttacttc ctaaatttat gaaagtagcg
     1021 gatttagacg atgctccagg tttgatgttg gtaggaatcg ctgtcgtatt cattcctttt
     1081 gcagtataca cattaacaag cgtgctggaa aaactgcttg aacatgcgat tttacttaag
     1141 acagatcatg atttgacagt gtgaggtgcg acatggcaat tatcgttaat ttagatgtag
     1201 tattagctca aaaaaaaatg agtgtgactg aattgagtga gaaagtgggc attacgatgg
     1261 caaacatctc tattctaaaa aatggaaaag caaaagccat ccgtttttcc actctcgaaa
     1321 aaatctgcca agttttggag tgtcaaccag gtgatttgtt gacctataaa aatgactcga
     1381 atactatacc agaagaataa aataaagtag aacaaacagt aaataaaaaa taatctgaat
     1441 gatagatgtg tgagactttt agaagttatt tacgtctatt attcagattt tttatttcga
     1501 ttgaataccg ttttatctat aaataagata tgtactgact catacaaagg gggccttttc
     1561 ttctccatgt actatcactt ttcctgatac aactggtttc ctgttacgtt gtagttggat
     1621 ctgcttttag tataatactt tattcattcc accagactga tagcagtttg agcagtctaa
     1681 ataatcatgt agtattacag gccattctgt ttcatacctt gaagtgctta aaacttcagc
     1741 taatggccca ttgtgttaac tcttactcca ttctttttta gtaagctccc atatatactg
     1801 taaagccttc tatatattat gtcatgttgc tttttaacat ttactatgta gttttgataa
     1861 aatgatttga ataacagtga tggacaattc ttttttattt ttaacattat tttgataata
     1921 atgattattt atttaaaaat acattagata acatttgtta cgacacgaaa aagcttcaca
     1981 aacaaatatc gttattttaa aatgagggcg aactcacaca attaggagga atagtatgcc
     2041 atttattgtt tacttttttc tttcattact cgtgatctat attccattac caacattttt
     2101 cctgctcgtc aatcgtctag cagagcaaca tgatacgatt gctgctctta cgaaactttg
     2161 tttacttcta tttgtttgtc tagattactt tacgagctat tattggctaa aacagacaag
     2221 agtaaaaaag agatattatt tcttccttgt ctttttcaat ctctttgagt ttctgatcca
     2281 ttgcttttta cttcatcagt atcatacgat ccatttgtgg atcatctggg ttttcaaagt
     2341 ggtcgtatta ttttgtatgt tcacctttcc tatctcaaga aaatttaggg aggcattgtt
     2401 ttattaaaga agcttctctg tatcaataaa gacacattta aggaaaaact tttaaaaatc
     2461 taattgggaa tttgcccgca taaataagag gctgtgacaa aagtcttgtc acagcctctt
     2521 attccgtata aacagtgttc aaaagcttgc atcttcgata atatgcccaa ccaaatgaaa
     2581 aatatgagaa gctatttttg tttggctgtt cttattactt gatgaagaac agctttttca
     2641 cgacctcttt ttaaaagtct ttttacagtt ttcgagcaaa gaatgatacg attagtacta
     2701 aaatgatggc accaatcaat gatggcacga ttgccattcc tgcaatactt ggtccccaag
     2761 aacctaataa tgcttgacca agtgctgatc caactaaacc tgctacgaca tttgcaataa
     2821 tccccatcga agaaccttta ttcgtgattg ctcctgcaat tgctccgata attcctccga
     2881 cgataagtga ccaaataaat cccatgtttc gttcctccta atgttttact tccttattac
     2941 tgaacccgtg gttctgtatt ttcttttact ttttgtgcgc cgcttccagc tgctgatttt
     3001 actttatctg tttgattcga agcaaattca ccggtttttt tcgctgcgtc agttactttg
     3061 tcttgcaccg tttcttgatc tttttcaaat tcttcttttg ttttgatgtc cactacgtta
     3121 gcgttgactt cgatcacttc taaatgtgtc atatttttta cttctttgcc gattacttct
     3181 tgcatttctt caaagatttt ttcgatatct ttgccatatt ccacaatgac atccatatca
     3241 actgctactt gttttttacc gacttctgtt tcgatcccag ccgttacatt atctgtattc
     3301 acaagttttc ctgccacatt agagaagaaa ccaccgtttg cagataataa accgtctact
     3361 tgttcaattg cgattccgat gattttttgc acaactttat cttcataatt cagctcaccg
     3421 cgcattgaat cttcctgttc agaatcatgc tttccttggt tatttgtatg atcagaacca
     3481 agttttcctt gtccgtttgt gtggtcagaa ctaagttttt cttgtgcttt cgcatggtca
     3541 gaaggatgtt tttgctcttc cacaggttta gaaggatcga ttgcatcatg ttgttctgga
     3601 actttagatt ctggagttcc tggatgatga ggtgcatgct tttctgcatc atgtttcttc
     3661 tcttctactc cttgttgaac gttagggact actggatctg gatgtgcaga tactggtgct
     3721 gttttgttct tttcatcgtt cacaccattt gattggtttg gtgtcgttgg gtctggatgt
     3781 gcagacactg gtttttggtt gttcatttta ttgttattat ttttgttatt gttattcatg
     3841 gatgtttcct cctaagattt aaaatagtga tctaagattt tcgtttgttt cagataaaaa
     3901 ccgatagcga cgccgatcgc tgccatgatc aagatcaata aggtcttgaa aaagcccacg
     3961 gtgatccata aaacggctaa tatcaatcca agtaccccaa agatgatagg gagcttgtat
     4021 gtcgaaaata gttctttcat ttttggtccc cccctattct actcgtttgc ggtcattgct
     4081 tcgtttagct ggttcttcaa agcctgtgta tttcaccgat actttcactg gctcttttgt
     4141 tcccaatatg tgttgtactt cattttcgat ttctttcatc aatgttcctg ttttaccaat
     4201 gagtgctgaa gttcgtttca actccccttt tacattgacg gcgatcttgt ttttcgttgc
     4261 tttgacattt acttttggtg aatggatgaa ttcatcttca tgcaagtgag aacgaaccat
     4321 tccttcaatt gcttttttat ccaacgtcaa ctccccatca ccatgtttca gtagaaagtt
     4381 tccgattgtt tttggataaa acaggaaaac aagtaacgtg ataattgtca aaatagcaaa
     4441 tgcaacggca agccaaaata catattgttg cacgtatgaa ttagtgatgg aaaagaatcg
     4501 aaacttctct agctgaaaag gcaatggata ggttgcactt aatagtatcg caaacaaaag
     4561 cacagatagc aaaagtaatg ttccgatcac tccgatcgtt tttacacctt tattcatttt
     4621 cgcacctcct cataggggtg aaacgatcaa cttcttataa gttgatcgct tctccaccag
     4681 ttactccgta aatttgggct gtaacataac ttgctttatt cgatgctaag aaaacataaa
     4741 ctggtgctaa ttctcctggc tgacctgcac gttctagtaa actttgctga ccaaattcag
     4801 ggatcgaatc agaaggttgt ccttcgtcta gttgtaatgg tgtccagatt ggaccaggtg
     4861 ctacaccatt tacacggatt cctttaggtg caagctgttt tgctaaaccg atcgtaaagt
     4921 tagcgattga agcttttgtt gctgcgtaat ctaataaatg ttcgcttgga ttgaatgctt
     4981 gaacagaagt tgttgttacg attgctcctc ctgctgggat atgcggaaca gcttctttca
     5041 ctaaagcaaa catagcgatg atgtttatca taaatgtgtc tttgacttgc tcaagtggta
     5101 actcagcaat agactgacaa gaaatttgct gtcctgcgtt tagtaccatc gtatctagcc
     5161 cgccaagttc actcacggct ttatctatga tttctttagg tgcttgttcg tcacgaagat
     5221 caaaaggtag taagacaatc ttacgtcctg catctttcac gtattttgct acttcctctg
     5281 catctttttc ttctcctggg aagaattgaa gagcgatatc tgccccttct ctagcaaatg
     5341 caatcgctgc tgcacgaccg atccctgaat ctccaccagt gattagtaca cggcggtttt
     5401 ctagttgatt attgccttca taggattctt ctccacaatc aggaacagga atcatattct
     5461 gctgtaaagc tggtgtatcc tgatcttgtt taggaaattt ctccgtatgg tacaagtttc
     5521 gcgggtcgat taattttggt tctgtcattg ctgtattcct cctagttttt aaattgtatt
     5581 gccaaaacaa atttctttgt ttgatacctg ttttaaatag attctttaat ttgtataatc
     5641 tttccggtat aaatggtaac ctaaaactac tgctgctatc aaagagccaa agactacgag
     5701 tataaagcct agagcagaac ttttacccgg taatcctcct gtattcattc cccaaatccc
     5761 tgcgaacaac gccggaacgg aaatgattaa tgcagctgaa tctagatatt tcatgatatg
     5821 atttagatta ttgtccatca tcccgttgaa tagatcacca atactctcta ataaatctcg
     5881 atagatctcg atcatttttt ctgtttgtcg ctgtctcaaa tggacatcat agagtagctg
     5941 ttcatcgtct aaacgtttga tgaaagtttc agaattccat aattgatcca ttgtttcccg
     6001 ttgatctctt aaggtatgat cgatatatac gatgtctctt tcaagatctg cctgtttata
     6061 cagttcttca ttttccgttg ttttacgagc tgctttatcc aaagcgtcaa ttcgcttttt
     6121 catctctctt agttctttca cgtaatgggt gtaaatatct aataatgtat acgtgatgat
     6181 cttttcaaac gagtttaaat gatttccatg ctttgtcagc agccgatcca caaattcggt
     6241 ttttctatcg atacaagtaa tcaagagatt atcggaaagt acaaaagata ttggcgttaa
     6301 ttttttttcg atacgattat ctgactgatc tagattaatc actaccaaag aaaatggatg
     6361 ctctagtttc gtctcataaa ggcgctccat ccgagaaact tcttccggat attcggttcc
     6421 tacgaaaata tcatcaggaa gctcgaattc ttcgattgct tgctcaatct cttcgggctt
     6481 tgcactatca agtacgagcc aattatattt tttttcatct gtttctatta cattcttttc
     6541 ttcatccaca aagtagtatc tcaatgtata cctccttttt ggactttctc gaaactatta
     6601 gctgaagaag cagttcttta atttgatatt ataatcgagt actgcgattt cattttctaa
     6661 tgaaaagctg tcaagctgtt tgttgaagtc catgttcaat cgtttaccga aataattttg
     6721 ctctaattta gatgtataga tgtccaataa tggatgctcg attttagtcg catattctcc
     6781 aaattccgaa acaaaaggaa tgatttgaaa aaagctgcct tttgcaaaag cacctttgtc
     6841 ccttagaaat ggatcggaat cattgataag cgcgagtgcg atttttgtat tttccccaac
     6901 cttttcaatt aacgtctcta ctgcattttt agacattgta atttccattc ctactccccc
     6961 tcttcagttg ttgtcttcta ttatttaaca actacatctg tttttggttg aacttttgca
     7021 tattttggat atttagggaa tttagctaca caacggaaag ccgtcatccc tgcatatttt
     7081 tcgtaatatt tcttcgttgg cccttcaaaa tccttgctcc acggtttcac atgtcctgaa
     7141 aaatggataa tgtaaggatt gttgcgagtt tcttcgtatt cacgttcacc tgctgctgtt
     7201 ggatgttttt ttgcttttgc catgatatag ccttgtgcat tccaacgcgg atgaagtggt
     7261 aaccaacggt catgtaagat agcatttaat gcatcttggt catggaatcg taatttttct
     7321 ggattatgtt cgatgaaatc taatactttt tgcgtaatat tttcatctaa ccattttttc
     7381 acattgatca gcatcaaacc tgagttgaaa taacgcattg atttagctga aattttcatt
     7441 ttttccagtc gttgatggaa cccagcgtct tcaacagctg ctacgattga atcaccgaag
     7501 tcaagacgcc ataatttaga gatatcttgt aaagcgatca tgtcgcaatc catatataag
     7561 atacgttcaa cttctgtgcc gcggaacaat tccggaatcg caatacgata ataagctgtt
     7621 tctggaatac gatcgctgat taccacattt gtaaagaatt ctttattgat tttcaagaat
     7681 tccacatctg agttcattcg tgcgtttttc actgagaagc gtaaaagctg tttactttca
     7741 aaatcgatat catcgtcaat cacatagaac cgaacaaatt tagacgggtt gcaattatct
     7801 aatactgaga caaataatgc agctaaatgt ggtacgaatt ttgtgttaca acttgcgact
     7861 actgctattt cttttttatt cattgatctc gcctactttc gttagagtac tgtgtgctaa
     7921 tttttttaag tataaattgg tatacggatg atctttcaac gtattccaag gtttatcatg
     7981 tcctgtaaag tgaacgatcg aaggtttttc atttccttct ttatactgac gttcatattt
     8041 ttcatttggt gctggatgac gttcaaaaat cagcgaagtc tgcatgttcc attttggatg
     8101 aagttgttcc caatcttcat aaagtacagc attcaaagca tcttggtcgt gatagatgat
     8161 tcgatcgcca ttttcactga ggtaatggat agttttttct gtaatttctt ttttattcca
     8221 ttgatcaatg tcaatcacca tcactcctga attgaagtac aagtcatcgg aatcgattcc
     8281 taatctttcc aaagctttca cttggcctgg atcgataata gcgccgattg tttttccatt
     8341 taacggctca tcatatagtt ttacgatatc gtctagtact aacacatctg aatctaagta
     8401 aaggactttc ttataatcct ctttagctaa taagtttggt aaagaaattc ttaagtaagc
     8461 ggtagttgtg atatggtcac tgactaggaa gtcttcgtag atatcttttt ccactgtcag
     8521 gaattggata cttgcattac ttgaatattt atttaccgtt tcttccaatc cttctttact
     8581 atattctgat agaccatcgt caatcacata aaattttatg cgtcttgttt tattgcaatt
     8641 ttctaatgca gttgcgatca tcacgctcaa gtaaggagca taattttcgt ctgaagcagt
     8701 taccacagga actacgccgt atctcttctc catcaaaatc actccttttt ctaatctttt
     8761 tctaacctgt tctacatcta gaatattcct catttggttt taaagcaaaa tatatgcttt
     8821 gttttcattg aatattttaa aattttttta tcacaaaacg catcggtaag cgctaactat
     8881 tcgtttttca tggcttttta ttcaaaggta ttgaccaaaa actactatat atagccacct
     8941 ttttcttaag aatgtttttt gaaaaaattc ataatgacaa agcaagttag tttcattttt
     9001 ttaaattctc atttaatctt aaggtaacag gaaaatagat atgtaaaaag aaagtaggaa
     9061 gataaaatga aactagcagc gattgattta gacggtacct tgcttgatcg aaatggaaag
     9121 ataccagaag aaaatatcca agcacttcgg aaatttgatg aaaatggcgg aatcgtaacc
     9181 gtcgctaccg gaaggaacag catttctgct aaagatattt tttccgaatt aggaatcggt
     9241 ggctatttga tttcttccaa tggttcactg attgctgaaa tgaaggatgg aaaaatggcg
     9301 aaagttctca gacgttcaaa aatagatagc gatacactga gaaaagcctt cttctatgct
     9361 caagaagcga aaatatccat cattgctagc cgagaaaccc aagatgatca gctgacattc
     9421 gatgaatcaa atctagtcaa agatgaccct tattatgaac atttcaattt acagaaccat
     9481 tcttttgatc aaattttaaa tatgttggaa gatccttctc ttagctatct taaattagca
     9541 ttaacagata agagtgaaga aaaactacag aagctacaaa gagatctgca agcagatggt
     9601 atccaatcag tattctctga tccgcatttc ttagaaatca cgcctacagg tatcactaaa
     9661 gctcattcac ttgtttttct ggcggattac cttggaatcg aatcgagtga catcatggct
     9721 tttggcgatc aagaaaacga cattgctatg ttgaattaca gcgggttgag tattgcgatg
     9781 gggaatgcac agcgacaagt aaaagaaatt gcaaaccaag taacactgac gaatgaagaa
     9841 gctggtgttg cttatatttt aaaaaacttc tattaaaaag aataaaatga cgatatgaat
     9901 gtaggaggaa agaaaatgtt agttattatt gatatgcaaa atcatattct tgatccaaac
     9961 agcgaatttt atattccaga gtcagatcaa ttggcagaac gtattgctca aagattagct
    10021 aaagcacgcc aaaataatga atatattctc ttcacacggg atataccagt tgaactgaag
    10081 gacaaagaag aagaacgaga agatttgcaa ttgatccctc ttctttctcc acaagaaaat
    10141 gaacgagtga ttaaaaagta ttactttacg attccacctg aaactttagc tgaaatccag
    10201 gaaactttat ttgaaagcaa agaagaacaa aaagagatcg aagtagtcgg gattgaaaca
    10261 aacttgtgtg tactctctaa tctgataggc ctgcagagtg cttttcctga agcagacttt
    10321 tttgttgatc caatgttagt ctccagtaga aaacacggag aatccgcttt agagctgttg
    10381 aaagatttca acgtctctat cgtcgaatca taaaaatatc tatcttcatt tacgatattt
    10441 aacacacaat aaaaaaagca cgcagaactg ttttagtcct acgtgctttt tctatcaaag
    10501 tagattggct tttttatgac acagagtgaa acggcaggct cacgacctct ttcttaaatg
    10561 gtgttcaatc agctgaccct cggtattagc ttaacaaacg gcaaaatatg aggaactatt
    10621 tttccgtttg ttaagctaat actcaggtca aaacgctgat ttcacaacct cttccttaaa
    10681 cggtattcaa aagcttgcat ctgcagtaat aagaacagcc aagtaaaaac ataagaagct
    10741 gtttttactt ggctgttctt attacttgat gcagggcagc tttttcacaa cctcttatgt
    10801 accttgtagg actcgaacct acgaccggac ggttatgagc cgtctgctct aaccaactga
    10861 gctaaaggta catcataaat aaaatttatt tttgttactt ttcaactata gcacgaatga
    10921 caaactctca caagtttcaa aatataagta aaataatatt gttattctta tttcttttac
    10981 ttgtgtcata actattttgt tactttaatt tttatttgct tattttagaa aaaatgttat
    11041 tcttttattt attctcaatg ttaggagtga ttattttgcc atttattgcg attttattcg
    11101 atttagttgc ggcagcagct tattttctcc aattaaatta tcaaacctca ggaattctgt
    11161 ttattggaat gatttttcaa ggtgtgatta cccttttgct tcttgtcatg atgctcagct
    11221 ataaaggaaa aagatatgcc cgtattcaaa caaaagtatt tgttaaatat gtttccattc
    11281 gctatgggat catcattctt tcctttttag tgaatgcgat cgtcctcttt ttatatgtat
    11341 tgaattactt agatatcaat ccattgattt tttctcgata gataagacac attcagcgga
    11401 acagttcctg cttatcagca gaaaactgtt ctgctttttc atttttcatt gatctattct
    11461 tcttttattt tcctagcatt tcatttgtga tgtatgcaat aacatgttat cttttattat
    11521 atgaatcgtt gaggagtccg ttacatgatt acgttggtga gagttttgtt ttgggtgcct
    11581 gcggtcgcac tggtcgcaag tattgtttat cttatgaatt ggaacaaaga acggttttat
    11641 ttagctattt tgactcttcc ggctatctac tttatgtgga aagtttttaa ttacaactac
    11701 tttgaacccg atagtgtttt tatagaagaa
//
LOCUS       DACDEG010000073        11655 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.077, whole genome shotgun sequence.
ACCESSION   DACDEG010000073 DACDEG010000000
VERSION     DACDEG010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.077"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1419
                     /locus_tag="IV142_002042"
     CDS             <1..1419
                     /locus_tag="IV142_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP8485692.1"
                     /translation="LADGQKGDNRLKTLKEIQQIYPQIPESKGGFPGYGGIPITHYKK
                     YWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAERQSSMVVNDPKTELYVASKDE
                     LEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDIDTATQLTNTFTNTIYYDANAS
                     DNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNNLVELLSEIGGFNYQDPDNEFK
                     QLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNILSTAITGLNPFTLTKIAKMTSQ
                     STIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHQGKKFHEERVKVGYRGFCEINFDCH
                     LEQGDHVCLKYDHRGKEYVAWYEFSQRVLKDEEGNIVYRKKRGETHLPELEKEAVLTL
                     DEDKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHIIVSIFLSQLYKELSTQCVKTKG
                     DKCHRRIHFILDEFGNSVTCLA"
     gene            2114..4030
                     /gene="ltrA"
                     /locus_tag="IV142_002043"
     CDS             2114..4030
                     /gene="ltrA"
                     /locus_tag="IV142_002043"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8485693.1"
                     /translation="MERDKTMTNTKGISLKNKQLRHAEYYDMTETFDELHAMSVAKSN
                     FKNLMKFIIDDNNIFLAYRNIKKNKGSATAGYDGVTIKDIEKLACGDFLTIIKRRFKD
                     YRPRKVKRVEIPKPNGKLRPLGIPSIWDRIVQQCILQILEPICEAKFNKHSYGFRPNC
                     STENAIADCLARINQSKMNYVVDIDIKGFFDEVNHTKLMRQIWTMGIRDKQLLVIIRK
                     MLKAPIVMRNGNVVFPTKGTPQGGILSPLLANICLNEFDWWIANQWEERTVKELKHHY
                     RKNGAWDKGNIYSKLRSHTSLKEMYLVRYADDFKIFCRTGEEAQRAFYATQKWLRERL
                     KLPISMEKSKVTNLNKAESEFLGFSIRLHKKGKKKVAFTYVCRKAEENIKNKLKQQIK
                     KIQYAGAKKNRHRAIGDYNMMVIGIHNYYRIASHCSVNFTEIYRSVRTVIEARLKARG
                     MTKNGIYGQDHEGYRPYLKSKQMRYIEGFPILPIGYIKKRTPLNKRRNLNKYTKEGRK
                     LIHQEQQAIESWKIKYLVKGFSNNKKYTVEFSDNKISKFISQKGKCAITGNELLMEEI
                     ECHHINPYHLSKDDSYKNLVIISREIHRLIHATSEETILKLLRLLKLNHYQIRKLNTY
                     RKKAANFPISIKMN"
     gene            4147..4947
                     /locus_tag="IV142_002044"
                     /pseudo
     CDS             4147..4947
                     /locus_tag="IV142_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            4944..7316
                     /locus_tag="IV142_002045"
     CDS             4944..7316
                     /locus_tag="IV142_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485694.1"
                     /translation="MIIGGIFSGIADWWSGLFGDGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKIKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLENDPNKVEHIPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNVAKNVGSGAKNLASAGKSGLSNLNDIRKNGFGN
                     RKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTAE
                     DLTEKAKDTLEEKLDGTRAKELAEAVKNPSETLEKQKEKASEVKEGLKDAYGEGQVNA
                     AVKANTTTEKENQEGAAIPSAKDHQDELHSNENSEEGQNGQLPKTDPEQYRYKSNINS
                     ENEKRISDLPKRNQEVQTDDDSIDLPNDNGVEVGTEKSTKIGLPKAQNLPMKDSRLNQ
                     FKENDQSFVHQSTAGNVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAP
                     EPIQRVHTGYKPANVAFTAPTKINVTRETKNRNNELKEQLLRDIKQDIKP"
     gene            7368..7700
                     /locus_tag="IV142_002046"
     CDS             7368..7700
                     /locus_tag="IV142_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485695.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            7701..8294
                     /locus_tag="IV142_002047"
     CDS             7701..8294
                     /locus_tag="IV142_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485696.1"
                     /translation="MRVKGQNSVDKKSIFQFGKPEERSILDLFPYKSCTEEGVLVTKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFIIEGRTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            8457..10481
                     /locus_tag="IV142_002048"
     CDS             8457..10481
                     /locus_tag="IV142_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP8485697.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIKNLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            10481..>11655
                     /locus_tag="IV142_002049"
     CDS             10481..>11655
                     /locus_tag="IV142_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8485698.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMNF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAMKHWGEGGNDNGTITGEWTNPFPGSSLDKSTFSGGQLFGTNPGS
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     "
BASE COUNT         4161 a         1880 c         2436 g         3178 t
ORIGIN      
        1 ctagcagatg ggcaaaaagg agataaccga ttaaaaacgt taaaggaaat ccaacaaatt
       61 tatccgcaaa taccagaatc aaagggaggt tttcctggtt atggcggaat accaattact
      121 cattataaaa agtactggta tattcagacg gatacagtaa atacttgtat tgttggtact
      181 tcccgttcag ggaaaggaca gatagaagtt ttagcaacca ttgacaattt gtctcgtgca
      241 gaaagacaaa gttcaatggt tgttaatgat ccaaaaacag aattatatgt tgctagtaaa
      301 gatgaactag aagctagagg gtatgatgtg tatgcattta atatcaccga tccactgcaa
      361 tcaatgagtg ataatccatt ggcactcatc gtgaaatact ggaagcgagg agacatagac
      421 acagcgacac agttaaccaa tactttcacg aatacgattt attatgatgc caacgcttca
      481 gacaataaat actttaacga aaatgcgcaa aaagctgtca atgcgcttat ttttgcgtta
      541 ttggaacaag cggatcagac aggggattac tccaaattaa caccgaataa tctcgtcgaa
      601 cttctttctg agattggtgg ttttaattac caagatcctg ataatgaatt caagcagctc
      661 aatgctttag atgaatttat gaatcagtta ccacctggaa acatagcaaa aaaacaatat
      721 ggggcaacaa aaattggttc tgataaagcg aaaggcaaca ttctatcgac agcgatcaca
      781 ggactaaatc catttacact gacgaaaatt gcgaaaatga cttctcaatc aaccattgat
      841 tataagtcca ttggttttcc gaagtatttg gatctcaaac tacacgagag tctgttaaac
      901 cagcgaattg ttgtagagtt ttatcatcaa ggaaaaaaat ttcatgagga acgtgtcaaa
      961 gtaggctata gaggtttttg tgaaattaac tttgattgtc atttagaaca gggagatcat
     1021 gtttgcttaa aatacgatca tcgaggaaaa gaatatgttg cttggtatga gttttctcaa
     1081 agagtattga aagacgaaga gggaaatatc gtttatcgaa aaaaacgagg agaaacccat
     1141 ttgccagaat tagaaaaaga agccgttcta acattagatg aggataaaag taacttatta
     1201 gtagagtcta ttcggatgca ctataccgat aaaccaatgg ctatttttat gctcacacct
     1261 gattatgatc catctaacca tatcattgtt agtatttttc ttagtcagtt gtataaagaa
     1321 ttatctacgc aatgtgttaa gactaaagga gataaatgtc accgtaggat tcattttatt
     1381 ttagatgaat ttgggaatag tgtgacctgt ttagcctaag gataaaagag gctagttcca
     1441 acgaacattg gaataacttt actatcgaga aatcaaatga cggggtaacg ccctgaaggg
     1501 tttccactga tactgcgaca tgcgttagta ggatgtgcat ccaaacgcgt atgaagctcg
     1561 ctgaagtcgg cagaagggta ccgtaaggaa agacacgata cgtatctacc acgcaagtgc
     1621 tctagaggta gggtatgtac ctgatatgtc gggtgtccta aaaagttcaa taaggtgaga
     1681 atgatttgat tatctgacaa acttgcgaat ggaatgtctg aaacgaataa ctcatagaaa
     1741 tgtgaggggg aagggggata cgtcctcttt ccattgactc gaggttgagt aaaaatgtgt
     1801 ttctatgaaa aaccttgcat ggttaaaggc tatggagtag tcaacaggca aaagcaagca
     1861 cctaagttga aatctatgga tagatagtaa ggaacagggg aagcatctag cacggaggga
     1921 gcataccgaa gatgtgtttc taaggaaaaa ccgaaatcgg ttataactta ggtgaataga
     1981 tgtgaaagta gagccacgac cagtgaaaat ttcgtaatga aattggagga acaggctcaa
     2041 gtcgttattt ataatataag aaacctattt aaaatcatgg attctcgtaa gactaaaaag
     2101 gtcacgaacc agaatggagc gtgataaaac catgactaac acaaaaggaa ttagcttaaa
     2161 gaacaaacaa ctcagacacg ccgaatacta cgacatgact gagactttcg atgaattgca
     2221 cgcaatgagt gttgcaaaat caaattttaa aaatctcatg aaattcatta ttgatgacaa
     2281 taatatcttt ctagcgtata gaaatatcaa aaagaataaa ggaagtgcaa cggccggata
     2341 cgatggtgtc actataaaag acattgaaaa attggcatgt ggtgactttt taactattat
     2401 taaaaggcga tttaaggatt acagaccaag aaaagttaaa agagtagaga tcccgaaacc
     2461 aaacggaaaa cttcgacctt taggaattcc ttcaatatgg gacaggatag tccagcaatg
     2521 tattctacaa attttagaac caatatgcga agcaaaattt aataagcata gctatggatt
     2581 cagaccaaat tgttctacag aaaacgccat agcagattgt cttgctagaa tcaatcaaag
     2641 taagatgaat tatgttgtag atattgatat aaaaggattc tttgacgaag taaaccatac
     2701 caaacttatg cgtcaaatat ggacaatggg gattcgagat aaacaactcc ttgtaattat
     2761 tcgtaagatg ttaaaggctc cgattgttat gcgaaatggc aatgttgttt ttccaaccaa
     2821 gggtactcca caaggaggga ttttaagtcc actgcttgcg aatatatgcc tgaatgaatt
     2881 tgattggtgg atagctaatc aatgggagga acgaactgtc aaagagctga aacaccacta
     2941 tcgaaaaaac ggagcatggg ataaaggaaa tatctattcg aaattaagaa gccacacaag
     3001 tttaaaagag atgtatctgg tacgctatgc agatgatttt aaaattttct gccgaacagg
     3061 agaagaagcc caaagggctt tttatgccac acaaaaatgg ctcagagaaa gattaaagct
     3121 cccaatttca atggagaaat ctaaagttac gaacctaaat aaagctgaaa gtgaattctt
     3181 gggtttttca ataagactcc acaaaaaggg aaaaaagaaa gtggctttca cgtacgtttg
     3241 tcgcaaagca gaagaaaaca ttaaaaataa actaaaacaa cagattaaaa aaattcaata
     3301 cgctggagca aaaaaaaata ggcaccgagc aataggtgac tacaacatga tggtcatcgg
     3361 tatccacaat tactatcgga tagcttcaca ttgtagtgtc aatttcactg agatttatag
     3421 aagtgttagg acagtaatag aagcaagact gaaagctaga ggaatgacga aaaatggaat
     3481 ctatggacaa gatcatgaag gataccgtcc gtatctaaag tctaagcaaa tgagatatat
     3541 tgaaggtttt ccgatacttc caattggcta tattaaaaaa agaactccac tcaacaagcg
     3601 gagaaatcta aataaatata ccaaggaagg gagaaagctc attcatcaag agcaacaagc
     3661 tatagaaagc tggaaaatca agtatcttgt aaaaggtttt agtaacaata agaaatacac
     3721 ggtggaattt tctgataaca aaatatctaa gtttatttcc caaaaaggga aatgcgctat
     3781 cacaggaaac gaacttttga tggaagaaat tgaatgccat cacattaacc catatcattt
     3841 gtccaaagac gatagttaca agaaccttgt cattatctct agagaaatcc atagattaat
     3901 ccatgcaact agtgaagaaa ctattctgaa actgcttcgg ttactgaaac taaatcacta
     3961 tcaaattaga aaactaaaca cgtatcgaaa aaaagcagct aattttccaa tatcaatcaa
     4021 aatgaattag ataggaaaca aattatattg taattaacga tggaacgccg tgtgcggtga
     4081 aagtcgcacg cacggtgtgg accgggggaa aagacggaga taatatcaaa ctcttaccta
     4141 tcggaattgc caccaatgga taatatggaa ggaatcatga cagtcacagc tggtagaaac
     4201 atgttgtggg atttgtttat ccaatcctat cagcaacttt atgcgaagta tggggaacaa
     4261 aacgcagcga ttatcaaaga aaattgtcag acacatattt atattatgtc gacagacgat
     4321 aataccatag aagaattttc caaaaaagtg gggaataaga cggttgaaca agagaacagt
     4381 accatcaatt cattaggtat gaatactcat atcaatcgca atgttgattc agatcgcatc
     4441 ttaactcctg aaagaatttc tactttttta gaaggtgaaa cgattgtact cgctccttta
     4501 aaacgacaag atttgaaagg tcataaaata cggccgtatc ctatttttaa tcatggggaa
     4561 acgaatcttc cttatgctta tcaatatatt gaagatttca atccatcgtg ggatatcaat
     4621 gatatagaca tacattgtac acatgcggac ttggatctta aggctttaca aattgattac
     4681 gattattttc tacctgatat ctatgcacgt cgactttatc acaatatgca cgatcaaata
     4741 gaagaggaag ctaaagaaaa catttttatt aaaatcgttc aatctttaga caactcaacg
     4801 gcacaaaagt tacttattca attttatgaa gagaagcaaa tagatcgttt gcaagaatac
     4861 cttgaaaaat tgcgtcaaaa tgaagaaatt acagaggtta taaggaaacg acttttgcta
     4921 gaagctgaaa aggcggtgaa agaatgatca ttggaggaat attttccggc attgccgact
     4981 ggtggtcagg cttatttggg gatggggatg cagatcccaa aaccgtaacg aaagtcttac
     5041 aagattatgg taattatctg cagtatcaag atgtattcag ttggatttgt aatacttttt
     5101 tatggggttt gattaaaggg ttatataatc tcaatacttt actagaaaaa acaatttatc
     5161 aaagctttga tttaaaagac ttattgaacg ccgctggtgt taatgaactg tttcaaagtt
     5221 tgattagtaa ggtattggct cttttttgtg tcgtcacatt aatttattta ggcttaaaat
     5281 tttttatgag taaacatcca ccaaaaatca aaaatatctt ggttaatcta tttatggcca
     5341 tgattttgat ttttggtggt agttctttaa tcgatgaagg gctaaatatt agtaaaaact
     5401 tttacggaga cgtgactgca gcaaataaag cagataaaac tggttcacct gcttttcaaa
     5461 ttattcaaaa taatgtgatt gatgtgagta cgttactaga aaatgatccg aacaaagtag
     5521 aacacatacc aacagacaaa agaaatgctc tcacggcgaa aaattttaaa acagcgaata
     5581 tcaatgggat tattacacct gatcaagcta agaaaatgtc tgaatcaagt gatattagta
     5641 cagtacaaaa agatcggtta aaggcattac agtatcgact agagttagac gataatggga
     5701 aagaaatccc tgttcaaatt agtgatgaag gtttagcaaa atatgtgtat acttcaggct
     5761 atcgccgata cattagtagt ccaggtatta ttttagctgg tgaatcttct ttgtcggtag
     5821 cctatttatt tatcttgttt acgattgtta cttgcattat tgagctggta tttaaaaaat
     5881 tctatttagt gattgccgcg tcaacggact ttgagacagg acaacggatg aaaagtgcta
     5941 tagaagatat ttctcaatcc ttcttgttat tggcatttac gattttagaa ttacgaatct
     6001 atacattaat gctttcaggt atcggtgatt tgcatgcggc aggaaagatc aatggattct
     6061 tgtatgttgt cgctttaatt gtattaacca ttgcgctatt caaaggctct caatcagtta
     6121 cgaaaatttt cggcgttgat acgagtctga aaaacggcag caattctctt cttagcatgt
     6181 ttgccttagg aaatgttgcg aaaaatgtgg gtagcggggc taaaaacctt gcaagtgctg
     6241 gtaagagtgg tttaagcaat ttaaatgata ttcgtaaaaa tggatttggt aatcgaaaag
     6301 atgttacccc tgataacggg gaagaacaat cagacgataa agctacaact gtacctaata
     6361 gtaagaaaac agtttccgaa atgttcggga aagcgaaaaa tggtgtttct aaagccgcag
     6421 aaggtcttgg ttacgtcaat gaacgtgggt taaaaggaac agctgaagat cttacagaaa
     6481 aagccaaaga caccttagaa gagaaattag acggcacgag agctaaagaa ttggctgaag
     6541 ctgtgaagaa tccatccgaa acactagaaa aacaaaaaga aaaagcttca gaagtcaaag
     6601 aaggactaaa agatgcgtat ggtgaaggac aagtcaatgc agcagtaaaa gctaatacaa
     6661 caaccgaaaa agaaaatcaa gaaggtgcag cgattccttc agccaaagat caccaagatg
     6721 agttgcattc taacgaaaat tcagaagaag gacaaaatgg tcaattacca aaaacagatc
     6781 cagaacaata tcgttataaa tctaacatta attctgaaaa tgaaaagcgc atttcagatt
     6841 taccaaaacg aaatcaagaa gtacaaacgg atgatgattc catcgatctg ccaaatgata
     6901 atggggtcga agttggaact gaaaaatcaa cgaaaattgg tttacctaag gcacaaaatt
     6961 taccaatgaa agatagtcgt ttgaatcaat tcaaggagaa tgatcaaagc tttgttcacc
     7021 aatcaactgc aggtaatgtt caaatgaaac caagtcgttt gcctgaaaat ggaaatctga
     7081 caactgtttc ttacaattta tctgaaagtg gggagacagg gaatgcgtcc gttcaaaatg
     7141 ttgtgacaag agaagctgca ccagaaccta ttcaaagagt tcacacaggt tacaaaccag
     7201 caaatgtggc atttactgca ccaacaaaaa taaatgtgac aagagaaact aaaaacagga
     7261 acaatgaatt gaaagaacaa ctattgaggg atatcaaaca ggatatcaaa ccataaaagg
     7321 cctacaagtt aggccttttt tctatgtatg aaaaggagaa atcatttatg gataaatata
     7381 ttaacatgaa aaatcctcca agatttagtg gggaatggaa gttttttgcc tttttctttt
     7441 tggatctatt aacgcttggt ggttgctttt tttgggcgaa aatagtcaat aaccttgtcc
     7501 atttaccact cttttttgca ctcttgaatt atggcttatg gattgcgata gctctagtga
     7561 ttgtttggcg accctataat aacccaggtc aacgacaaat ttttgcgatt atcgagtcta
     7621 ttttttttac ggacaagaac cattatcatg cgttagatcc taaccgaaat tatcatggta
     7681 cgtataagga ggatgaataa atgcgagtca aaggacaaaa ttcagttgat aagaaatcca
     7741 tttttcagtt tggaaaacca gaagaacgat ctatcttgga tttatttcct tataaatcct
     7801 gtacagaaga gggagtctta gttactaaag aagatcattt tcaacgttat tatcgtgtag
     7861 tttctacaga cgtggaagga ttaaatgaac aagagaaact agaacgaatg aatcagttga
     7921 ccaccgtcat gcgtacgtat gtgccaacaa ttaagttagt tagtttgacc acagagacgg
     7981 atttaacgga acaaatcgta caaaagcggc agttattaga taagaatcgt atggaacaat
     8041 ccttaggaag aaatctacag cgattaagaa aatatgaaaa gaaattagta gaggaaatcc
     8101 aggaattgaa acgagcagaa caagaacgtc ctgacttaag cttctttttc atcattgaag
     8161 gaaggacaat caaggattta caaaccagaa accgtcagtt aatgcgatct agtggcattt
     8221 tagggttaaa accactctac aaaaaagaat tgatcaagat tctgtatcga atgaacaaca
     8281 tgaatgatga gtaggaatgg agaagagaga atgaaatatc cgttgatatg gttagaaaaa
     8341 gatgttccga aggaagagac cactagaaaa tttcctcaca ctttaagcct gggtaagttg
     8401 ggaaaagggc aatcttttca ccaagtaaag aatcaaacaa gaagaggtga ctgtacatgt
     8461 tagaagcgat taaaacagct ttttcaccac aaaatgatca aaaagtactt gatccattaa
     8521 agcaactagg aaatcgggaa atagaaattt taaaatccaa aggatatgat ttagattttt
     8581 taaaacaagt gatgccacaa ggtggattaa atttcgatga agagtatgtg acctatggaa
     8641 atggttattc acggtgtgtg acagtttatc actacacaaa aaatcctact cttttttggt
     8701 tggccaatct catgaacaat cctcatacag tggccacact agatgttagc acagatgaaa
     8761 agcaaaaagt gattgatcaa atcaatcgcg cattagatga attaaatgat cgatcagata
     8821 atgaacgaaa agcgactgac cgaaataaaa gtgcggaaga atacctggag ctagcggaat
     8881 atgcccgaaa attgacttct aatggggaaa tttctaaaca agtcaaagtg agagtacatg
     8941 tttacggaaa cacgattcaa gaagtagatg aacgggcgca ggaactgatc aaaaatctaa
     9001 aagggatgga ttatcgtgcg gtgacttatc tgcataaaac caaacgagat tggcaatcga
     9061 tgtttaccat gttcgatgaa caagctgaat ggttcggaaa tatcaaaggg caatcactac
     9121 catcgttaaa cattggcgga ggtttcccgt tccaccacca aacgctaaaa gatccaggag
     9181 gtacacaata tggggaaacc atgacaggcg gcccatttgt ctggaatgcg acgtataaaa
     9241 atagtgtacg gttgtctact aataccttgc ttctaggaat gatgggagct ggtaaatcga
     9301 ccgttcttaa aatgatcgcg gaagcccatt tagctgcagg agactttatt tggggctttg
     9361 aaaagggaaa agactttatt cctttcttaa aggaatataa cggtattatg gtgcgtttag
     9421 acggatctga cgggatgatc aatcctttag agatatttgc cacacggacg tatgatgaag
     9481 catccagttt atatgacgat ggatcagtca aagatttgaa gatcaatgaa gcagcgtcct
     9541 atcaaacaca cttagacaaa gtggtctatc aggtacagtt agtgagtcct cagctaaaag
     9601 gaacgatgaa agcagaattt aagacctact taaatcgttt ttatgaagag tatggaacag
     9661 taccacgcgg ctttacttcc tcaaattcgc gttcaaatac tgagacgcaa gtcacaggga
     9721 aagatccaga agcttatccg acgttcaaag aatttttaga ctttttagga caactggaat
     9781 taccaggtgc ttcccaagag aaaaagaatc gaaaagaaga aatggaatcc atcgtagaat
     9841 ccttatgtga gacgtatggc atgatttttg atggtcacag tacgattcga caccttgacc
     9901 aacaacagtt ggtctttttt gacattgact ctattacagg cttagatcga ggggtacaac
     9961 aatgtcaaat gtacatggct atgacgttga tttggaatca ggcattgatt caaggaagaa
    10021 gacaacgacg tttaattaaa gaaaaacaaa tggaagaaca agacttcaaa cgcttcttgg
    10081 cgatgattga tgagtgccat aatgcgatta atccagattt tattgaaacg gtaaattatg
    10141 tcacaaactt tcaacgagaa atgcgtaaag tagaagcaat gactgtttta gctacacaat
    10201 ccccacaaga aatggtgcca gaaaatatgt cttctgataa cttctcgaaa ctgaagaaag
    10261 tctttgaatt ttcttctacg aagatcttta tgcgtatgga tgaatctatt ttacgtcata
    10321 tagaaggatt agttggtaaa tcaatgacga actcagaact agaatccatt cctcaacaaa
    10381 atcaaggaga cgctgtgatc aattttggct caaaagaaac cattcgtgtt cattttagtc
    10441 cgaacaaacg tcaattaaaa ttgtttgatg gaggaaaata atggaaaacc aaaatgaaag
    10501 tttaatcaag cagtatgtta agcgtcgtgc aaagcgacgc ttatttttgt ggttgtttgg
    10561 tacttctgca ggtttgatta ctattttgat cactgtgttc gtgacccttt ttctaatact
    10621 ggcggcgggt tcgattgata attctgattc agattcaagc agtggtggcg aagcttttac
    10681 aggagaatat tcggaaggat tacccattta taaagaaatc aaaggaagag gccctttttc
    10741 tgatgagatt gcgcaatatg ctgtaggtgc agcagtgaaa tataaattat taccaagtgt
    10801 gatcctttcg caatacggtt acgaatcagc gtttggtacg tcggcgtccg caagaaatga
    10861 tctcaactat tttgggatta cgtggttcga tggctgtttg tttcctaaag gaactgcacg
    10921 aggaattggt ggtattgaag gtggctggta catgaatttt cccaactcaa aagctgcatt
    10981 ctcctattac ggctttatgg tagccacaca atcaaatttt aatgcatgtg taggaaataa
    11041 aagccctggc gcaagcttat tgatcttagg acgaggtggt tatgccgctg caggaattac
    11101 tgaagatagt ccgtattata caggttgtat gtcaattatc acttctaata aattaaccga
    11161 atatgatgaa ttcgcgatga aacattgggg tgaaggtggt aatgataatg gcacaattac
    11221 tggtgaatgg acgaatccat tccctggcag cagtttagat aaaagtacct ttagcggtgg
    11281 tcaattattt ggaacaaatc caggcagtga attccgtccg aatggcttcc atgatggatt
    11341 agattttggt tcagttgatc atccaggaag tgaaattcac gcggtgcatg gaggaaaagt
    11401 cgtgtacgtt ggcaatcctg gtatttcagg tttaggtgct tgtgtgatcg tgattaatta
    11461 cgacgggttg aatatggtat atcaagaatt tgcgaatagt acaggaaatt ctcgtgtgaa
    11521 ggttggagat caggtgaaag ttggtcaagt aatcgggatc cgagatacag cacacttgca
    11581 tttaggtttc actcgaatgg attggcgtca agcacaagga cacgcattca cagatgatgg
    11641 tacatggatt gatcc
//
LOCUS       DACDEG010000074        11486 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.078, whole genome shotgun sequence.
ACCESSION   DACDEG010000074 DACDEG010000000
VERSION     DACDEG010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.078"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            293..499
                     /locus_tag="IV142_002050"
     CDS             293..499
                     /locus_tag="IV142_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP8485699.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(701..1702)
                     /locus_tag="IV142_002051"
     CDS             complement(701..1702)
                     /locus_tag="IV142_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8485700.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1707..3619)
                     /locus_tag="IV142_002052"
                     /pseudo
     CDS             complement(1707..3619)
                     /locus_tag="IV142_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
     gene            complement(3787..4293)
                     /locus_tag="IV142_002053"
     CDS             complement(3787..4293)
                     /locus_tag="IV142_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8485701.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4453..4893
                     /locus_tag="IV142_002054"
     CDS             4453..4893
                     /locus_tag="IV142_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8485702.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4919..6076)
                     /locus_tag="IV142_002055"
     CDS             complement(4919..6076)
                     /locus_tag="IV142_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485703.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6079..6435)
                     /locus_tag="IV142_002056"
     CDS             complement(6079..6435)
                     /locus_tag="IV142_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485704.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6733..7707)
                     /gene="sgrA"
                     /locus_tag="IV142_002057"
     CDS             complement(6733..7707)
                     /gene="sgrA"
                     /locus_tag="IV142_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP8485705.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7903..8742)
                     /locus_tag="IV142_002058"
     CDS             complement(7903..8742)
                     /locus_tag="IV142_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8485706.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8927..9814
                     /locus_tag="IV142_002059"
     CDS             8927..9814
                     /locus_tag="IV142_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP8485707.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10408..11103)
                     /locus_tag="IV142_002060"
     CDS             complement(10408..11103)
                     /locus_tag="IV142_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP8485708.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(11087..11485)
                     /locus_tag="IV142_002061"
     CDS             complement(11087..11485)
                     /locus_tag="IV142_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP8485709.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3427 a         2292 c         1721 g         4046 t
ORIGIN      
        1 atatgattgt tatgtaaaat aaaaaagttt aggatacatc gaatttttgg ggaaattctt
       61 ttgggggtat cctaaatctt ttctattata ttcccatatt ttctattatt ggtctagacg
      121 tatgatggta aactttgtac ctatctgatt gctgaatcat taattgctta tcattagcca
      181 tctttgtttc aagttcgcta gatgatcatt ttattcacat cctaacaaac ttgtcacatt
      241 tatagattct taatctattt atcatacaat tataaaaaag gaggtgctgc ttatgaaagc
      301 tatccatatt tctttgcttt ttttcgctag tgcattgatt ttatttgcta ttcatctttg
      361 ttcccagcct tatatcaata acaacggaat attagtggaa ccagcattct tttgcttacc
      421 actcggcttt ttgtcccttc ttgcctccgc aggcttcgga tttgttgctt tttttaaacg
      481 atcaaaacaa caaatctgac ttataaaatt tacgtaaaag agatcgtgac aataatcgtg
      541 tgatattccc aaatttcgaa taaaaggtgc cccttgccat ctctaactcc tcagtcacaa
      601 gttacaatat tcataaacca tgaaaagctt gtttattgaa attgctcctt gtgacttgga
      661 gctaagtgac agattacaac ctcttttgca tcataagact ttactccttg aaaaacatta
      721 ctaacttttg atatgtttct tgcgcttgtt tattgctgaa atcaaactga tattcatggt
      781 tcaaaacagc atttttctcc gcataatcta cttttgtgac tttcaccccc gctttctcaa
      841 atacagaagt tacttcttcc atttgtttcg taaacgtatt tgtatttcca tccgttaaga
      901 atgtgttttg cgtccaacga tccgcatgtc tgaccaaggc taaacttttg accttctctg
      961 attgttcaaa gtccttatta tcaaagtaag cccatccaca tttttcataa agaaagctag
     1021 agattttatc acttacttga gtaagtgcag aaggatccaa cagaccagac aaagaaataa
     1081 acttcgtaat ctgcttgttc tccaaagtcg gatcaagatg atcagctttt ttaatttctt
     1141 cattttgaag tgctaacaaa tattctccac taatttgtgc acctgccgaa tttccaccaa
     1201 tcgaaatatt cgaccaatct gcttgatctt ttatctcttt tttagctgct tgaatggctt
     1261 cgttcaattg gataactggt gtaggatgtt ttgcttccgg tgatcgttca taatctatat
     1321 ttacgatgat ttgctgcgtg tctgaacata gatattccaa ataatcacga caatcatttt
     1381 tatcccctcc tacatatgcg cctccatgca tccagaaaag aatcggtaat tttttctttt
     1441 ccactttcgg tgtgtagata tccatattat tatttttgta ctttgaagga taggatattt
     1501 ctttttttat atcgatttgt tcgatttttt gctggaaatc atctggtcgg gcatatactt
     1561 gttctttttc acttgtgtca aattgttttc taacaagcaa gctaatcggc tttggtgtga
     1621 tttgattaat caaaaataca gtgactaaaa taactacgat gataactggt actgcaatca
     1681 ctattcgctt gaaccatttc attcatttac cctctctttc ttcgccaaat ccatgtgata
     1741 atcgtgctaa cagataaaaa tccacctgaa taaatgagaa gcgaatgttt cttttctcca
     1801 gttttcggca acattctttt tttgcatctg tctttttttg tactttcact gtcatctgaa
     1861 tcggttgcgt atcatcactt gattgatcct ctcgaggttc attctttccc ttattgtctg
     1921 cattctctgt cggatcttgc ttttttggtg gagctactgg gatttttttg taatagaaaa
     1981 taatagttgg tatcgtctca ttaaaaatcc cttcagtcgg tccttcaaca tttgctaatt
     2041 cgtatccttc gaatgtcttt tgctctgtca tataattttc tccataagct ccgtaaagct
     2101 ggatttcgtc ctggtctttg agtagacttt tattgttttc atccacatac tttacgttta
     2161 tctgatcatg gaacactact ttcaaaaagt ccgttacatg tttcgtgtag gcagcatggt
     2221 caacttcctg tgcctttcca tgccctgctc cttctactaa taaaagatct ttataccctc
     2281 ctgataactc atataatttt tgcgcgttag acacaggtac tacatcatct gctgttccat
     2341 ggataatcaa tttaggcaat gaatctgaca ttccaatcgt aggtagagca gcttttgtat
     2401 ccaaatgcat ctcctgatca aaatagtatt gtttcaaaag gcccataaat aaatcttcat
     2461 gctcttgatc cccaatgatg tctaactgat tttttatggc atccggtagt gcgggggcta
     2521 ctgtctggct gtacgtaaaa cgtagttgtt catcaattga tgcaaatccg caattttcaa
     2581 taattccttt gacattgaca ggaatatctt tcgctaatac agacatcatc gtagctgctc
     2641 ccatcgaacc accataaagt aaaatctttt gagatggccg ctctcgaact tcttgattta
     2701 tccagtaaag gacgtcatct gactcatatt gtccataggt cacataatcg cctccgctat
     2761 taccagtcgc tctggaatca acaaataata aattataccc ggccttataa aaatcattgt
     2821 attcttctgt aacgatcccg ttattccaat tccctcgaaa tccgccatgg atcatgactg
     2881 ttttatctga accttgatcg acataatatg catatacatc catcattttg ccatcttgtt
     2941 tgtttagtac agtttgatga agttgttttg tatttggata gaagcctgtg ttttctgtag
     3001 gcgttgtccc atcatctgta cctccaagca tcgacatcaa cacactgttt tttgcaaaag
     3061 cgatatcgta caatagataa gtagcaatca gatgtttgat aatctcttta tctacaccat
     3121 aatcattaac agcaagctca gtgactaata actctattaa ttttgacttg atgtcttctg
     3181 ttggtccatt aagtatttct gtcatctgtt taccgaagta attcaaatat tctttaccat
     3241 tgaaattttc acctttaccg atatcatcat tttctgtccc attctctttg tcacttactt
     3301 tagcagaaga ggattcgtcg tttgacattt ttgtagctgt tgcttcttct gtttgttggt
     3361 tatctgtggt agcggtcgtt tcttgatctg tgctagtaac agatgtctca accgttgttt
     3421 cagtatttgt ttctgtttgt gaagtactcg atttcgtttc atttccagca tcagtagttt
     3481 ctgtggtttc tacctcccga gattccgatt cggaagaaat gctctctcca tatactgtcc
     3541 attgcgggac gcttgtactt acagcaaata agatgaccaa cgctcccttt aatacttgtt
     3601 ttgttctcaa gctattcact cctttttatg tttctaggag aattatatgt aaacgcaaac
     3661 attttttata caaaaaataa atggtttatc ccataaaaat agaacaggat tattttctca
     3721 ttttacgtaa taaagcagaa tggataaacc agtatccatt ctgctttttt taaacgaaga
     3781 aacaacttat tgctccaaaa atgtcagaaa tcgtttatcc caaattttct cgtaaaaagc
     3841 gattgtttcc tttgctttca taaaaggatt atcataagtt gtcgttgctc ctctacacat
     3901 cataagctgg tatcctaatc catgagcaaa cttcacagtc gtatctatac aatactctgc
     3961 ttgtgcccca cataattcga tcgaatgaca gtccaattca gttaataaaa cactcaaatt
     4021 agtcttataa aatgaattcg catgggtttt tctcacaaaa taactgtgct caggaataga
     4081 taattccgac aatagctccc atggttttgt ttgtggcact aattgagcat cttcgtgttg
     4141 aacgaaaata actggtttcc caatcgtttc gtatgttttg attcttttat tcactaatgc
     4201 tgtcactgtt tctaattcgt gtatcggcga agaatcaaaa catacccctt tttgtaaatc
     4261 gatcactact attgcgtcgg ctgtgtttga catacttgtg ccttctttct tttttcttga
     4321 tttaatagta tttcttatct agctagttcc attatacgtg acaacatttt cttattttac
     4381 acctctaaat ggcacttttc gactatttct tcatgttatt atttcataaa tacttagcta
     4441 ttggaggaat gtatgatgat taaattgtat acaggttctg gctgctcgtc ttcaagacgc
     4501 gcaaaacgtt ggcttatcgt ccatgatata ccgtttatcg aacagaactt aattcgtcaa
     4561 ccactaacca aaaatgaatt tatccactta ctaaaattga caaacaacgg cgtgaccgaa
     4621 atgctctcta ctcattctat tagttataaa aatcttgttc acagaataga tgaactcagt
     4681 ttgagtgaac tatatattga gattcgtaca tccccttctt tattgaaaaa accgattatc
     4741 tacgaccaat ctgcaaaact tttgactgga tttagcgaag ataaaatcag gatgttcctt
     4801 tctaaaacta aaagaaagca agagctcaac gaactcttgc tttctggtca tcttgtcgat
     4861 tttaaagaaa ataatctttt tgcaatgtct taattttggc ataaacaatc tgcttttctt
     4921 aattaacgaa atccaatttt tccaatatgg atctattttt ctcattcctg atttttatga
     4981 tttcagcttt cagacgcgcc caatcatttt ttgtaggttg tccattccag taaaatatct
     5041 gtgtattcgt ttgatcatat gcctttttgt cgataggata atcgctaatg acaatatcag
     5101 gtaactgatc agcggatgca ctatatttta ttgggcgttc agtcatagat aatatccttt
     5161 tctttgccca taattcctgt gttttaccat actttgattg aatatagaat atgacaggtt
     5221 gttcgtgttt tataaaaata tccctgacta acatacagta ttgaaaaatc aaccgtctgt
     5281 tcttttcata ataagtatta agctctggaa ttctgcgagc taccccacta aaaaactgtt
     5341 gcatgattgg atacatcact ggaaatatat gctgaaaatc tctgtcttct gctgattttc
     5401 caaaagcatc caccttcttt tttgtcccaa aaacccttga atacaaatgg atcattgcca
     5461 aattgacata tagataattt ttttctgaag ttgtaaaaca aatcttgaag tttttttcaa
     5521 aattttctac aatagacgat tcaatccagc cccaactttc ttgtacttta tctggagtat
     5581 ccaacaaatc ttcttccaaa taggtgttca ttacaccaaa cataaaataa aggtaagctt
     5641 gctctgttgg agtgatgtga ttcatcgacc agaagtttgc ttcaaaaaaa ggctctagta
     5701 tgtaatgcgt aaactcttct tttgttatca atggatgcac aaatttgttc atctcttctg
     5761 ggacatacgt aatcgagtgg ccttgtttca ttcgttgcaa cgaaattctt agtaggctga
     5821 tccatttctt tctatctata ctacgcgtat atggcgcatg tttcgcaaat ttttctaata
     5881 gttgacgttt attttttata cttgataata tatcagaatg attttcgtcg aatgactgcc
     5941 aatacatcaa gtgaaaaaag tatcggatac ggtattcttc tccttctatc ggattacctt
     6001 ttttcaagct gcaagataaa tgatagtttt tcaaaatagt attaagttct cttaattttc
     6061 tttgaatcgt acgcaaactt atataatttt ttgactaaga ttttctaagg attgataagt
     6121 acctttgtaa agtgaatcca gtaaaatata accaatcgtt ccctctaaaa gtatagaata
     6181 aactttactc agtaaaaact gagaagaaaa ctcgactttt atcatcttgt tttctgaaag
     6241 aaaagataat ttatattctg gatacctttc gcaaaacgat tctgcttgtt gaatcaccga
     6301 attcatctta gccagggtgt agtttagatc taaagcgata tccttcacca catataattt
     6361 ctcgtctctt tcataacaat agttcaaaaa ttgatatact tgcctttcat tgatatcaat
     6421 aaattcttca aacaaagtac atactccttt ttatctatta tttagactta ttccgacagc
     6481 tacatgaata acgtacaaat agattcgtta ctgatttttc agagatacta ccatgcgcag
     6541 tgtgcattgt caattattct tgctcttatt ttccatttgt gttcctgtta tttcctgttt
     6601 ctcaaatatg ttagtaaaca aaaatagatc tggcaaagct acaacttcgc caggcaatac
     6661 agcactctcg aatgaaatat tcactcaaaa atctatcgtt ttattcaatt ttagatctgt
     6721 tgcttttatt ctttatttca ctcgatggaa gagagaacga aaatagcttg ctcctacaat
     6781 caaaatgagt aacacaccca tccatgtaat tagcttgttc aaggttctac taccagtttc
     6841 tggtaaatcg ctttttctat tatctgtttc tttcgctttt tcttgatgat caattccacg
     6901 agaaacaaaa tcaggctgtt ttagcacact ttttaggttg ctccctgcat ttctcttttg
     6961 ttccttagtt ggtatgatag gtaccaaatc aagttcatta tttacttttc ctgtgctgct
     7021 ttcgctgctc gtgcttcctg tgctactctc actactcatg cttcctgtgc tactttcact
     7081 actcgtactc tccgtactgc tttcgctgct cgtgcttcct gtgctacttt cactactcgt
     7141 actctccgta ctgctttcgc tgctcgtgtt ttctgtgcta ctttcactgc tcgtactctc
     7201 tgtactgctt tcaccgctcg tgttttctgt actgctttca ctacttgtgc tatccgtact
     7261 gcttggcggt ccaacaactg tccaactgct tggtttcaaa ttctccgtga tcgtcatctg
     7321 tccatcataa aaaacaatca aatcaacttt atctttactc ttgatctcat caatcgatag
     7381 ttctgcagaa atcattattg tatactcaat accagcggtc cattgtacgt cttttaaata
     7441 aacttcccca ataacttttt ctgaacttcc attaccatcc caaccatatg ttagaacagc
     7501 atgtacatca ctagcattca cgcttttgtt tggtttgact ggaatagtaa tgacttgttg
     7561 tgtcatgtca taaacaatcc gatcagtttt cacagtaaat gaaagattct catttgcccg
     7621 ttcatttgca gaagtactga ttccaatcat tgctgcaatt atactcagcc caagaataac
     7681 aaataatacg gtcgctgttt tcttcatttc tatcactctc catataatta ccttctattt
     7741 gataaaggaa ttatataaaa tgaaaatgat gagaacacga cagtcagttg tgaaaatcta
     7801 acaaaagatg tcgtgtattc gcttgaattt gtttactttt tcgtttaatc aatgacagac
     7861 ttaaaaaagc aagaccgata tcagccttgc tttcttcatc ttttattgat aaacagcagt
     7921 gtatttcttc atccgctttg ttttatatgc atagagtaac aaaccaacaa tcaagactaa
     7981 caagaataat gtagttgata tagatgcttc agccccaaag tttcccccag taattattgt
     8041 attttgcgat tggatcagag tggagaaaaa accttgttct tgcgttccgc tgactggtat
     8101 acccaaaata gctccttgtg tccaattcca agtaatatga gcaccaatag ccgtaaataa
     8161 acttcctgta ctgtagataa gtgcgctgaa aaagcaacct gctaaaaaga tattgaaaac
     8221 tgccagaaat gtcatgttag gattcaatcc gtgcaaaaga gcaaataatg cagcagatcc
     8281 aatcattgct attaacggac caaatctaag catctgatgc tgaaaaaagc cacggaataa
     8341 aagctcttct gatgccccca ctgcaataaa taaaataaaa ctcgttatca gcggcacaat
     8401 gtttgcgttt cctgaatcta accgatacgc ccccgtcaaa tataatggac tagtaataag
     8461 cagaaacact ccaattccaa caacgagacc tcccgctaaa tacccccagt cttttcctcg
     8521 aacagaaaca ccataatctt ctattctttt tccttcgacc ttactagcaa gaaataaagc
     8581 tagtaaaaaa acagctaaat gtccgaccgt accggcaatt gctccaatcc attcactatt
     8641 tccaataaac atatccgcaa gtgcattatt gacaagaatt cctaaaacaa aaccaactaa
     8701 gactattaaa acagatccaa tcaaccgaat aatcctgatc atctttcatc cctcctttat
     8761 attaagttta acaacccttt tctttcttgt ctcgtgaaaa ctatgtaaat gttctccatc
     8821 atagagagaa tagattttta cgaactaaga atatctacag caaccttacg aaaatgaaag
     8881 actgtctccc taattcttct gttagactgt ttttaataag gaaaggatgg gaaatttttt
     8941 gaaaaaacaa ctactaatta tgtgcctttc agcatgtgca attttactat tctcaggttg
     9001 tagcaagtcc aatgaaacga ttgcaacgtt taaaggtgga acacttacag ttcaggattt
     9061 ttatgataac ccaaaagtta aatcaaataa tcaaacacta ttaaaaaaaa tgattgtctt
     9121 caaagcattt gatgatgtat acggaaaaga gatttcttca aaggaagtaa caaaagaata
     9181 taatgaacaa atcaaaaaac tcggtccaaa ctacaaggaa caattaaaag cagttggcca
     9241 aacagaagag acatataaga aactctttaa acagatgctg gctttccaat acggtttgaa
     9301 agccaatgta aaattaacag ataaagatct agacactgct tggaaagaat tctatccaga
     9361 agttagtaca cagatcattc tattttcgac taaagaagaa gcagataaag caaaaaaaga
     9421 agcaaatgaa ggagaaaatt tctccaagct tgttcaagct tatggaaaaa ataaaacatt
     9481 aaaggaaact gatggaaaaa tgaattttga ttcaaccaat ccagagattc ctactgaagt
     9541 aaaaaaagca gcatttaaac taaaaaacgg ggaagtttct gatatcattc ctgtaacaga
     9601 tccaacgact tatcagcaaa gctattatct agtaaaaatg gtaaaaaaac aggataaagg
     9661 ttctaataaa gacaaataca aatctgagct agaaaaaatt gctaccgaag caaaaactct
     9721 tgatacggaa ttcatggata aaacaatccg taaagtgatg aaaaaagaca atgtaactat
     9781 taaagatcct tatgtaaaaa atatattcaa ataaacaaat ttgacccaat tggggatgtg
     9841 gcaaaatttg agaagtgatt tttggaaatt ccttcttatt tttgaggcaa accacttttg
     9901 ccacaacttc atcggggcct ttttttattt gtaccaaaac aaatcactgg atttgttaca
     9961 atcatgagga tataatttaa aaaagttttt tatttctatc acatgaatca atgcgccatt
    10021 aagtagattc tttcatgctt ccctattttt tcacttgccg tatcgcttca aaaacaacta
    10081 tcataaactc aaactcgact gaaaagttat tttaaaaaag actaaaaata tcccttctgg
    10141 acttgttctt cttattctgt tatactagag aaacacgaaa gatattttta tgcgacactt
    10201 ttcatttgtt tttatgaaat ttattcaaaa ctggcgtaac ttcagagtta caaggatgta
    10261 ttagaggtta gggaatacat cgtttaaatg agaaataagt aatagttagt aatagtcacc
    10321 caaactttaa attaatgaat attgaatcgt cagatagcct cctcgagttt tcgagaaggc
    10381 tatcgtttat tttatggaga aattgattca gaagtaatca gcgacctttt ttagttcacc
    10441 aatcgtttct gcttggaaag tttgcaccca atcttcttgg aagcccctca ctgctttatc
    10501 aatcgagggc atctctaata cttgatctag tagctcttta cctaacgtta tcgcatgagc
    10561 tccagatcga atggctgtag tgacttgttc acaatttttg aaactagctg ctaatatttt
    10621 tgttggtctt tgcgtacgtt cgatctcagt ccgcaacgaa tcaatcactt tcatagcatc
    10681 aatgccttga ttttgcatcc tgttataata aggtgcaata taatctgctc cattattaat
    10741 agctaaatac ccttgtaatt ctgtgtaaat agctgtaccg gtaatcaaat aaccttcttt
    10801 tttcaacttt ttgatcgctt gaaggcctgc ttgattcacc ggcaccttga taaacgtatc
    10861 ttttcccagt tcttctatga taactcgagc atcctctatc atttcttctt ccgttcttcc
    10921 tattgtctga atatggatag gaatctgtcc gattattttt ttgatttctt tcatatgcga
    10981 gaagaagttt gacgctcctt cttttttcag aattgtagga tttgttgtta cgcctgttaa
    11041 attcaaaaac tgttgacaag aataaatact ttttgtattt gctgtatcaa gcatgatttc
    11101 catatagctt tcccccattc tgatcctctt gatcgatttt aatgattgtc ccctttctta
    11161 aatgcggcat ttccttatct tctacataca ttgttcctgg aagttccggc tcatgagatc
    11221 catcaaattt tatcgtgata tgtcctaatt cattcaaatt agtttggaca acatctccta
    11281 ctgctgtgat tacatacatt tgtttatcaa aacagagctt catatcacaa gttatttctt
    11341 ttcttacctg attcaattca atcttataac aataatctgt taatgtttcc ggtgcttcat
    11401 ctccaaataa aatcattatt ttttctttcc tgaatacttc tgcttctata ccaacatctg
    11461 ttacaaccgt ttcaaagatc gtcatc
//
LOCUS       DACDEG010000075        11390 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.079, whole genome shotgun sequence.
ACCESSION   DACDEG010000075 DACDEG010000000
VERSION     DACDEG010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.079"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(53..988)
                     /gene="murB"
                     /locus_tag="IV142_002062"
     CDS             complement(53..988)
                     /gene="murB"
                     /locus_tag="IV142_002062"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP8485710.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1061..1981)
                     /locus_tag="IV142_002063"
     CDS             complement(1061..1981)
                     /locus_tag="IV142_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8485711.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2094..2846
                     /gene="xth"
                     /locus_tag="IV142_002064"
     CDS             2094..2846
                     /gene="xth"
                     /locus_tag="IV142_002064"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP8485712.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3833..4366
                     /locus_tag="IV142_002065"
     CDS             3833..4366
                     /locus_tag="IV142_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP8485713.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4856..5380)
                     /locus_tag="IV142_002066"
     CDS             complement(4856..5380)
                     /locus_tag="IV142_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8485714.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5373..5846)
                     /gene="tsaE"
                     /locus_tag="IV142_002067"
     CDS             complement(5373..5846)
                     /gene="tsaE"
                     /locus_tag="IV142_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP8485715.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5969..6949)
                     /gene="pta"
                     /locus_tag="IV142_002068"
     CDS             complement(5969..6949)
                     /gene="pta"
                     /locus_tag="IV142_002068"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP8485716.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(7102..7782)
                     /locus_tag="IV142_002069"
     CDS             complement(7102..7782)
                     /locus_tag="IV142_002069"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP8485717.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7908..8777
                     /locus_tag="IV142_002070"
     CDS             7908..8777
                     /locus_tag="IV142_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8485718.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8803..9318
                     /locus_tag="IV142_002071"
     CDS             8803..9318
                     /locus_tag="IV142_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485719.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9365..9928)
                     /locus_tag="IV142_002072"
     CDS             complement(9365..9928)
                     /locus_tag="IV142_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8485720.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9983..10162)
                     /locus_tag="IV142_002073"
     CDS             complement(9983..10162)
                     /locus_tag="IV142_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485721.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10239..10748)
                     /locus_tag="IV142_002074"
     CDS             complement(10239..10748)
                     /locus_tag="IV142_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485722.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10844..11224)
                     /locus_tag="IV142_002075"
     CDS             complement(10844..11224)
                     /locus_tag="IV142_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8485723.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
BASE COUNT         3270 a         2333 c         2024 g         3763 t
ORIGIN      
        1 agcaggacgg ccaccgtcct gctcttttac ttattttaag ttccattact tattaattgg
       61 ctaaacttct gcttgtttct gatattgctt gtttttctcc aatgatccgc acttctgttt
      121 ctaaagacac atcaaatttt tctttgatta cttcttggat gtgcgcaatc aattcaatat
      181 aatctgtcgc tgtcgcatga tcgatattga cgatgaatcc agcatgtttt tcagagactt
      241 gtgcaccgcc ccatttcagg ccttgcaatc ctgcttgttg gatcagctga cctgtatagt
      301 gtcctactgg gcgcttaaag acacttccgc atgaagggta ttctaatggc tgttttgatt
      361 gacgcaattc ggtcaactca tccattcttg ctttgattgc ttcataatcc cctgtttgca
      421 aactgaatcg agcttccaat acgatagcac gtaattcttg gactttactg tgacgataag
      481 aaaataccat ttcgtctttt gtcaaagttt tcaatgttcc atcttcaagc aaaagatcca
      541 cttcagcaaa aacatctttg atctctccgc catacgcacc tgcattcata taaaccgctc
      601 caccgacact tcccgggatg ccgcaagcaa attcaaatcc agtcaatgat tctgccaaag
      661 ctgcataagt cgtatcaatc agtgaggcac ctgcttccgc aatgaccatc gttcctttga
      721 catgaatctg tttcatttca gtcagcatga tgactacacc gcgaatcccg ccatcttgaa
      781 cgatcagatt gcttgcattt cctaatacca tccaaggtat atcatggatt cggcaataat
      841 cgatgatttg ctttacttct tcttgttttt tcgggaatgc aaggacatca gctggtccac
      901 ctgttttcgt aaacgtatag ttcatcaatg gttcatcaaa caatatagta atcgtggcta
      961 agctttcaac tatttcttct ttattcactg aggattttcc tttcttttct caaacgtcct
     1021 cattattcta tcacgcttaa tacaatcttg ccagtcttgt tcaaagttga tgacggtagt
     1081 gatctttaag cacttcctca cataattcat gacttcttac aacttctttt tgtttaagca
     1141 attctgtttc cgatttacct aaacggatct gagaaaagaa ggcctctacc attggctcaa
     1201 acccgcgttt gactagcgtg tttgtccagt cgccaaattc tttcactttt tttccatcag
     1261 catcttgaat gacaagctgg gtcagatcgt ttacttcata agtaccattt ttacttgtta
     1321 cttgatattt ttctatatta gcgccacttg ctaaatccat cgttaaaata gccgtttgat
     1381 tttgtgtttc taattgtaaa atggcatatt ccaaaaattc ctttgtttct cttatctggt
     1441 attttgttct caaaatgggt tcatctaata aatacacagc tgtatcaata agatgtaaaa
     1501 acaaatcata aaggacgaac tctgtcgttt cttttgccgc tattcgattt ttttgcaggt
     1561 ggatcaaccg cttatctggg atacgtttca attcttctac cataggagca aagcgccggt
     1621 tgaaaccgat catcaaaatt atttggttct tatcagctaa ttcttgcaat tcacgaactt
     1681 catttagatg gacactcagt ggtttgtcaa tataaacatg gataccattt tctagacatt
     1741 ttttggctaa ttcataatgt acttttgttg ctgcgtggat catacatgct tcgatttttt
     1801 cttctaaaag ctcatcgatc gtttcatata gatgttggaa cccgtactct ttttgaattc
     1861 tttcttttgt ttctttattt cttgttgcaa aataaaaatc aagtgttcct tgttttttcg
     1921 cataagttgg taaatatgct ttttctgcga tattccctac accgattaca ccaatcttca
     1981 tctactttcc tcctggatca tttagttact ttcatcttac accatttttt actaaaatga
     2041 ggtacaatag tatcgcaaag gtaaggaaat taattcgaag gagtgtgctt ttattgaaat
     2101 ttatctcttg gaacgtaaac gggcttcgtg ccatcgtaaa taaaaatttt ttagaagttt
     2161 ttcatgagtt agacgccgat tttttctgtc tgcaggaaac gaaactccaa gcaggacaaa
     2221 ttgatttaga tcttccaggt tattatcaat attggaatta cgcggaacga aaaggctatt
     2281 ccggcacagc gatttttgct aaaaaaccag cattgaatgc cacttacgga atgggtatcg
     2341 atatccatga tacagaaggc cgtctgatta ctttggaata ttccgatttc tttcttgtaa
     2401 cctgttacac gccaaattct caaagcgaat taaaacgttt agattaccga ttagaatggg
     2461 aagaagcctt ttataattat ttggaaaatc taaaaaaaca aaaaccagtc atcgtgtgtg
     2521 gggatctaaa tgtcgctcat caaaagattg atttgaaaaa ctggaaaacg aatcaaaaaa
     2581 atgctggatt cactccagaa gaaagagcgg ctctttctcg actgttggat aacgggttta
     2641 tcgatacatt ccgttatttt tatccaaccc aggaaggtgt ctactcttgg tggaactacc
     2701 gattcaattc aaggaaaaac aatgctggtt ggcggatcga ttactttttg acgagcaaag
     2761 atttagagcc aggactagct gatgcaaaga tccatacgga cattatgggc agtgatcatt
     2821 gtccagtaga attagatttg aaataaagag gttgtgaaaa agtcgtttag tttcgagtat
     2881 taagaaaaat tttgtgaaaa tggctttcca cattttgcat aaatttggca taataccgag
     2941 aaactggctt ttgaacactg tttatcgaga gattgtgaaa aagctgtcct gcatcaagta
     3001 ataagaacag ccaaacaaaa atagttcctc atatttttcg tttggttggg cttattaccg
     3061 cagatacaag cttttgaaca ctgtttatcg agagattgtg aaaaagctgt cctgcatcaa
     3121 gtaataagaa cagccaaaca aaaatagttc ctcatatttt tcgtttggtt gggcttatta
     3181 ccgcagatac aagcttttga acaccgttta ttaagaggtt gtgaaaaagc tgcactgcat
     3241 caagtaataa gaacagccaa acaaaaatag ttcctcatat ttttcgtttg gttgggctta
     3301 ttaccgcaga tgcaagcttt tgaacaccgt ttattaagag gttgtgaaaa agctgcactg
     3361 catcaagtaa taagaacagc caaacaaaaa tagttcctca tatttttcgt ttggttggac
     3421 ttattaccgc agatgcaagc ttttgaacac cgtttattga gaagttgtga aatcagcgtc
     3481 ctgacctgag tattagagaa acaaatggca aaatagttcc tcatattttg ctattttttg
     3541 agctaatacc gagggtcagc tgattgaaca ccgtttagtg gagaagttgt gaaaaagtcg
     3601 cctactttcg agtcgtaaga aatttttttg aaaatggttc ctcacaactt tgcataaatt
     3661 tgacttaata ccgagaagtc atgatcccca tctaactcca aatcacaagg aatgagttca
     3721 aaaactagct tttctcaaca aatattacat aaaaaattct atgttttttg ttaaatttat
     3781 ttgctttttt caaaagtatg ttacagtaat ttcccaagga ggttttacag aaatgaattt
     3841 tgttgccatg gattttgaaa ctgccaatca tcagccttat agtgcttgtt ctcttgcgct
     3901 agtcatggta aaaaatagcc agatcgttga tgaattctat acattgatac agcctgagac
     3961 tccttttttc tggcgaaatg tccaaattca cggaatccat caagaagacg tacgcaatgc
     4021 gccgaaattc ccagaagtct ggcaaactat ccaaccatat tttcaaaaaa atcgtttagt
     4081 cgttgcacat aatgctgctt ttgatacaaa ggtcttagcg ggatgcttag attattatca
     4141 tcttgaacag cctaattatc tttctttatg tacagtgaaa agtagccgtc gcctgtttcc
     4201 tgaatttcca aatcatcgat tgaacactgt gtgcgaaaat ttgaacatcc aattagatca
     4261 tcaccatgat gcattggaag atagtcgtgc atgtgcggaa atcctgcttt atcaagaaaa
     4321 aaaattcggg acagatccat tgaaaaaact tgtgactata aagtaaaaga atacttatga
     4381 aacacacaaa gaggtcgtga gcctgccgtt tcactccgag tcacaaggag caatttcaaa
     4441 aaatagcttc tcatgtttat taaatattgt gactggtgac cgaggagctg gcaggagaac
     4501 accgtttatt ggagaggttg tgaaaaagct gttctgcatc aagtaataag aacagccaaa
     4561 caaaaatagc ttctcatatt tttcatttgg ttggacttat taccgcagat gcaagctttt
     4621 ggacaccgtt taccaagagg ttgtgaaatc agcgttttga cctgagtatt agagaaacaa
     4681 acggcaaaat agttcctcat attttgccgt tttttgagct aataccgaag gtcagctgat
     4741 tgaacaccgt ttattcaaaa aaaatggact gcgacacgac ttttgtcaca gtcccttcgt
     4801 gtgtttttct gccttctcaa aaggatgaca gtttctcact cttttcctat ttcttctatt
     4861 caatcaaata actcatttta tatacatcta aaaattcacc tagatctgtt cttgcccctc
     4921 taggcatgac cgcttcgatt tggaagccca tttttcggta aaggtgtacg gctctttcat
     4981 tacgaacttg aacgtcaagc tccaatcgaa aaagtacacc catttccttc gcccaactga
     5041 tgatttcttc tagcataaga gtgcctagtc ccattcccca atattcctgc agaatactga
     5101 taccgacttc tcccacgtga gacaaacgaa attgactgtc tgccttgaca gatgctgttc
     5161 caataatcgt cccttcatag attgcaagca gcaataaatt attattgctt tcgtatatat
     5221 aatccaactc ttcttctaag gtttctggtg gtaacaggag ttctgcttca tccaaaacta
     5281 aaaattctgt ctccttatta acttgtccca tcatcaaaag aatgtctttt gcatctcctg
     5341 gcactgcttc ccgcaagatg atcccaactt cttcactcat gattttcagc ccatttttct
     5401 agaattcgct gtttgaaggt ttcactttgc tccccgtatg agattagttt cacataccgc
     5461 ttttctaaat ctgtatcact tttttctaaa atcagttcca aataatcctc cggcagtgct
     5521 tcttctaata gatttcccca ttcaacaacg gaaagcccat caccttcaaa atattcatct
     5581 aaccctaaat ctgctccgct agctgctacc cgataaatat ccatatgata caacggaatc
     5641 cgaccttgac tatattctct aataatcgtg tatgtcggac ttttgatcat ttgcgttatc
     5701 cctaatcctt gggcaattcc tttcgtcaac gttgttttac ccgcacctag atcaccagtc
     5761 aatatcagat tgtcacctgg cattgcggaa tgaccaattg cctctgcgaa ttgttctgtc
     5821 attgttaatc ctgatagttc gatcattttt catcttctcc cgctcttctt tctttttgcc
     5881 agtatatcgc gtaatgaagt ttttttaaag aaaaaatgaa ctagtcaaga acagcagctg
     5941 ctgtttttgg ctagttcgcg tgtatttgtt aatcaatcaa tgtttgtgct gctgtgataa
     6001 tcgataattt atatacatct tcttcatttg ctccacgaga caaatcagaa accggtttgt
     6061 tcaagccttg tagaattggt ccgattgctt caaagttacc gaaacgctga gcaattttat
     6121 agccgatatt tcctgactgc aattctggaa atacaaagac tgttgcttga ccagctactt
     6181 ctgaatctgg cgctttcagt tgtgcaactg aaggaacata agaagcatca aattgcaatt
     6241 cgccgtcgat catgtattgt ggcgctagtt ttttagcgat tttagttgct tcaactactt
     6301 tgtctacttc tggcgatttc gctgagcctt ttgtcgagaa gctcatcatt gctacttttg
     6361 gttcgatatc gaacaattct gctgtttttg cagaatctac ggcgatttca gccaattctt
     6421 gcgcagttgg attcacgttg atcgcacaat ccgaaaagac atatttttct tgatctcttc
     6481 cacgtaccat gatcatcgct ccgcttgtcc gactgatgcc aggctttgtt ttgatgatct
     6541 gaagtgctgg gcgaacagtg tcgccagtcg aatggatcgc tccagagacc attccgtctg
     6601 caatccccat atacgtaagc atcgtaccaa agtaattcac atcttttaaa atcttttctg
     6661 cttgttcttt ggatgccttt ccatttctac gttcaacaaa agtatccacc atttcatccc
     6721 acttatcgta attatcaggg ctgatgatcg taaagctaga tgttttgatt cctctggcat
     6781 gcgctgcatt tcttatttct tcttcgttcc ctactaaaat gggttccaca agttcttctg
     6841 ctttcaaccg ggcagctgcg cctaaaatac gagcatcagt agcttccggg aaaacgattt
     6901 tgatcccacg gcgaacaatt ttaaatctta aactatcaaa caattccaca agatgacctc
     6961 cagacgtttt tatatgtatt atcatacact attccagaaa aaaagaacag tttattttat
     7021 ctgtatcagt caaaaatgct ggaaaaatta agtttttcac atacaaagcg aactgcacta
     7081 taacaggaaa gcgctgtttt attacactgt ttcaggcagt tgccaatcaa tcggttcttc
     7141 tcctaacgca aggagcgcat cattcgtttt cgagaagggt ttggaaccaa aaaagccacg
     7201 acttgcagat aacggactgg gatgtggaga tgttatgatg atatggcgac tcttatcgat
     7261 cattttgatt ttttcttgtg ccggttttcc ccacaagata aacacgattg gtttttcccg
     7321 ttcatttaat ttttcaatga tcgtgtctgt taatctttcc caacctttcc cgcgatgaga
     7381 atacgcttgc ccttcccgaa cggtaagaac ggtgttgagt aaaaggactc cctgtttagc
     7441 ccatgacact agatttccat gttgaacagg tgcgattcca agatcatcat aaagctcttt
     7501 atagatattt cttaacgatg gcgggatctt tacgccaggt tgcacagaaa aagataagcc
     7561 atgagcttga ttttctccat gataaggatc ttgtcctaaa atcaccactt taacttcttc
     7621 ataaggagtc aattccaatg cttcatagat atgatacata tctggatgga tcttttggga
     7681 cgcatattct ttttttaaaa attctcttag atgcagataa taatcttttg aaaattctga
     7741 ggacaaaact tcttgccaac tgttatgaat gatttctttc aaaaactcca cctcctaata
     7801 aaaagaataa ctcacatttt cttcaatcac aagggacaat cttcaccctc agcacttgat
     7861 taatatggta aactaggaac aagaagcaga aaggaacgat aatcattatg atcaaattaa
     7921 ttgcatcaga tatggacggc acattattag atgcccatat gagtgtttct cctgaaaatg
     7981 ccgaggcgat acgttttgca aatgatgccg gtgttgagtt catggtcgct actggtcgaa
     8041 actatcaaga agctcgcgca gctttagatg aggtaggaat cgattgtgcg atgatcactt
     8101 tgaacggggc acaggtattt gataaagaag gaaattcatt gtttaccgtg tcgattccaa
     8161 ataatcaagc aatctctgtt ttagatattc tagatgctaa cggtatttac tatgaagtag
     8221 caaccaacga tggtctttat tctgaaaatc aagcgaaacg aattgaaagt tttgcttcca
     8281 tggtggctac ccatttacct caccttacgt ataaaatggc gattgccatg gcctcagcta
     8341 agctggagtt attgcacatc acatacgtgg acagtattcg tgctattttg gaagaaaaga
     8401 atctggaagt tttaaaaatt atttgttttc atacagaagg gccagctgta ttaggtccag
     8461 ttggaaagca gatttctctt ttaggcgatc tagccgttac ttcctctggt caaaacaata
     8521 tcgaagtgaa ccataaaaac gctcaaaaag gaattgctgt tgcccatgtc gctcacgagc
     8581 gtgggatttc tttagaagaa gtcatgacga ttggcgataa cttcaatgat gtcagcatgc
     8641 tgcaagtagc aggagtcagt ttcgcaatgg gaaatgcaga aatcgaagta aaagattatg
     8701 ccaaatatac aacggacaca aaccttgaat ctggcgtagg aaaagccata ttgcgcgcga
     8761 ttgcagaaga cttgtagttt tatctagaaa agagggatat agatgtcaga atttttcatt
     8821 caggaaaagc agcttggacg tgcaactcgg acaattgtca aaaacgaaaa aggacattcc
     8881 ctgtttttga tggttggtcg ctggggaaca aagggcgatg cattgtctct ttatgccatg
     8941 aatggcgatc tcgttgctca catcaagcag ataagcctga cattcggtac gcgttttgag
     9001 ctgtataaag agttcgaaaa ggtgggaacc atgcagaaaa tattcaattg gcccggtgac
     9061 ttttattata ttcgtagact tcactggaca gtccaaggtg atatctataa tcatcaatat
     9121 caaatccatc atttcaatca gcaaatcatg aaaatggata aagcaacact tttttcagga
     9181 gattactatg tattggatgt ctcaaatgat gaagatgctc cagtttgtat ttgtattgct
     9241 gcggttttag attactggct ctaccgtaaa gacaaaagtc gcgataagcc tgtttcttac
     9301 cgacttaact taagttaaaa agatgtacct gaaatgcgga aatacgcatt tcaggtacat
     9361 ctttttagat attcttttgc atatgatcat aaagatgacc actgatcatc cgctgaccga
     9421 caactttgaa gtcatggcgt tcgtataatt ttttagcctt tggatttttt tcatccacac
     9481 ttagaccgat gacggagcga ttagctttct tcgccaattt aggtaatgct tctaataatc
     9541 gcgaaccgat gccttgtcct ctaaaatctt ctgatacact gatagagtcc aaataccatt
     9601 cgttgggaaa cgtttcttta tcaatgaaca aacgtgtaga cgcatctagc tgatatttct
     9661 ctaagatagg taccagtggc tgatcgatga ttgattcttc ttcatctgta taaccgtaag
     9721 caactcctgc aactttaccg tctatttctt ctacgatacc tcgtgaataa ctgtaacgat
     9781 aatttggatc agtgattgct tcttctaaaa tctctaaagt cttttgttcc cctgtctgct
     9841 tcaaaaatgg cagttccata tcttttagaa tcactaagat caatggggcg atttctggag
     9901 catcttcttt tctcgcaaaa cgaatcatag tttcctctcc ttttctccat tctaacatgc
     9961 agaaaaagaa atcgcaaaat cgttatttta aaaaatcttc aatcttttca gtaatgaaat
    10021 aaccatgagt aacgattctt gccgcttcag ttcggcaata agggcagacg actggcattt
    10081 ctttatcatt atgttcgact acattattga cttcttcgta tgcaaatttt tttccgcaat
    10141 tagggcattt cattttttcc atgttatctc aacttccttt catctttact ctcttagctt
    10201 actcttatat tgtaaaaaag agaagcaata tccttcttct atgcagatat ccatacctga
    10261 cggtcctgtt ctccatctat taatcggact ttataataag gacgttcatc tttgactaga
    10321 agtgtccact ctttcacttt tgcttttttc ccgtagtagc tgactgcttc cttcatcgct
    10381 gcttgcggtt gtttgaactc ttccgttttt tctatatcaa aaagatgttc tgcggccttt
    10441 tccgtgtcat ttcgtacggt tgttttccca gtggatggat tgactgctat gacttgtcct
    10501 tcacaaaaaa actggtattc atatgttgca ttcgtaggat tctccaaatc tttttcaact
    10561 gcaaaatgaa tagcagatat gttatgttct tttgtttcgt tttttacgat tgctgctgct
    10621 tttttcaatg agatccgata ttcctggtca ctaatatcat ccatgtgaaa agcggcattg
    10681 agcagtcccg tcataccggt aatccctatt acgatgaggg cagcaacacc aaacatcatt
    10741 tttttcattc ttcattctcc tgtttctttt ttttaaagaa tatcaagaaa tgaacgaatg
    10801 aaaaatcgaa tgaaacgaaa aaaaagtgac aattttgtta tctttattcg tgcagttcta
    10861 atggcaatcc gtctggatca aagaaaaacg tcattttttt tcctgtaaag gtatcttctc
    10921 taatcggttc tgattcgatc cctaaggact gcaattctgc aattgtttct tcaatatcgt
    10981 ctactttgaa agcaagatga cgcaagccac aagcttctgg atagtttggt cgttgtggcg
    11041 cattagcaat tccaaaaatc tccaattcag cacttcctag tttcagatct aatttatagt
    11101 catctcgttc tttgcggtaa ttttctctga ttacttcaaa tcctaattta ttaacataaa
    11161 aatcacgtga tttctgataa tctgaaacaa tgatggctat atgatggatc gttgtaaggt
    11221 tcatatgagc actcctattc ttcgtttaag ctcggatcta tattaatctc cagttccatt
    11281 gtaaatgtgt cagattgaaa aaaatagctg tttttccttt ttataagaaa aaaggcaaaa
    11341 aaaacagaaa gtaggtcctc caagacactc ttcctgttcc caatataata
//
LOCUS       DACDEG010000076        11341 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.080, whole genome shotgun sequence.
ACCESSION   DACDEG010000076 DACDEG010000000
VERSION     DACDEG010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.080"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(368..1486)
                     /locus_tag="IV142_002076"
     CDS             complement(368..1486)
                     /locus_tag="IV142_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485724.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     NVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1957..3264
                     /locus_tag="IV142_002077"
     CDS             1957..3264
                     /locus_tag="IV142_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP8485725.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3383..4582
                     /locus_tag="IV142_002078"
     CDS             3383..4582
                     /locus_tag="IV142_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP8485726.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4609..5877)
                     /locus_tag="IV142_002079"
     CDS             complement(4609..5877)
                     /locus_tag="IV142_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP8485727.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6040..6686)
                     /locus_tag="IV142_002080"
                     /pseudo
     CDS             complement(6040..6686)
                     /locus_tag="IV142_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7094..7597)
                     /locus_tag="IV142_002081"
     CDS             complement(7094..7597)
                     /locus_tag="IV142_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8485728.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7720..8484)
                     /locus_tag="IV142_002082"
     CDS             complement(7720..8484)
                     /locus_tag="IV142_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP8485729.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVISLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8592..8867
                     /locus_tag="IV142_002083"
     CDS             8592..8867
                     /locus_tag="IV142_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP8485730.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9305..10255
                     /gene="yidC"
                     /locus_tag="IV142_002084"
     CDS             9305..10255
                     /gene="yidC"
                     /locus_tag="IV142_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP8485731.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(10434..10634)
                     /locus_tag="IV142_002085"
     CDS             complement(10434..10634)
                     /locus_tag="IV142_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485732.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
BASE COUNT         3401 a         2386 c         2002 g         3552 t
ORIGIN      
        1 tttttaaacg gtgttccaga agtagcttct tcggaaataa gccgaaattt cacaaaaatt
       61 tgagaaataa tttttgtcaa tctcttctta tttcttgaag ctgaccactt ctgtcacaac
      121 ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagcccac caaacaaaaa
      181 atatgaagag ctatttttgc ttgctgttct tattacttga tgcagaacag ctttttcaca
      241 acctctcata cacggtgttt aatcagctga ccttcggtat tagctcaaaa aacggaaaaa
      301 tatgtgaaac tatttttccg tttgtttctc taatactcag gtcaaaacac tgatttcaca
      361 acctctttta ataacgaaga atcgctgtca gactcttttc atctggcgca tctttttgat
      421 ccaaaataaa cactttcccg ccattaatga tcacatcatc tgctaatgca ttcagcactt
      481 gacgccgatc atattctgtt tcaggatctt caccgaaacc atcagctaag tttgacgtag
      541 cgataaataa atgtgacact tttccttctt tcgcagctgg aacgatatca actaactgat
      601 cgatgaattt cttatctaag agtttattgt attcttttga ttcttgagct gcgagttcac
      661 ttgacagctt cttcgcccct tcttcgatat ctctaaagga tagctgcgca ggagaagcag
      721 aaatcgcaac gtctgcacaa taatagggat ttttagcaat ttttttgaac atcgtctgat
      781 tttctggtaa tgcaaataaa taaatcggcc attgttcagg attttcgaaa gaatccttca
      841 agtacgtatc aacagcttgg taatagttga cccaatcgat ctctacttct tcatcttttg
      901 gatttacgcc atggaaagtt actccatctc gggctccgcc tcctccatta gaagagaagt
      961 tcaaaccgcc acctgtcagt tcagatccca atgcagtaac gacattaacc ggtgcccctt
     1021 ctggtagaga tacttctttt acttgtttgt tgctgacctc atacaatttc atggagtcgc
     1081 ggttcaatcc caataaataa taagaatagt tgaactgccc atttttgata atagccagta
     1141 aataaggaag atcacttaca tagtattgat cgtctaccgg gatactcaaa cgatggatat
     1201 aggtctggtc agcagttata atgaccgcta cactcgtagt tgcactacgc caaaacgaag
     1261 gatctcctaa caaattttca attttttctt ggaacgggga ccagtcatgt tcggaatatt
     1321 ttttctcaaa acgtttcttt gcttctttcg caaaattttt gaatttgatc tgatcttttt
     1381 ccacatcttg atgggcgaca tgcgtattca gcatgatcgt gacgaatggt cctttagcgc
     1441 cttctgaagt caattgtgtc aataattctt tatccttttt tgccataaac gtacccctcc
     1501 taaaaaaatc attcctttgt ttctatctaa agtctaccac aacactattt ttccatacaa
     1561 taaaacgtat ctaaaaaaat aaaacggttt ctacggtttt aaatttagct atttttttaa
     1621 gttatcttca taatttcctc aattatttag ccccagtctt caactattct tcatgaaatg
     1681 ctgataaact aagaaaaaga ataaaagagg tggatcttat gcatttgatt atgattttca
     1741 gtttattaag tattatcagt ttatttgctt tcagcgcttc ccgcttatta gatgcggaaa
     1801 cacagcctat tcctgtaaaa gtacgtaaaa acgaccgcta aacttccata acgcgcgttc
     1861 ttatcctaaa gttttttcag aaacattgaa actctctccc ctctctgata aaattttaat
     1921 ttgtagcgaa acaaattaat tacaaggaga tcggatatgt ccataaaaaa aacacgtcta
     1981 gtcctattat tggctagtct cattctttgc cttgcttctt ttctaccagc tgttgctgtg
     2041 caagctgaag atacgttcaa agtaaatgca aaagcagctt ttgctgtaga tgcgcaaagc
     2101 ggtaagattc tttatgatca agatggagaa aaaaccatgg ggatcgcttc aatcacaaaa
     2161 atcatcggtc tttacatcgt ccttgatcaa gtaaaagaag gaaaactgtc atggggtgat
     2221 aaagtttcga tttcagatta tgcagaaaat ttgagtatca ccccagattt atcgaatgtt
     2281 cctttgcata aagaaaacac ctacactgtc aaagaattat tcgactctgc aatcatccag
     2341 tcagccaatg cttcaatggt ggccttggca gaaaaaatct ctggcagcga agctaaattc
     2401 actgaacgaa tgaaagaaca attgaaagat tggggaatca aagatgcaac aatcgttaat
     2461 gcatcaggat tgaacaactc ttatcttggc gaaaaccgac cagaaggcac tggagaaaac
     2521 gacgaaaatc aaatgtctgc tcaagatgtg gccatcgttg cccgccattt gatcttggat
     2581 ttcccagaaa tactggatgt atcaagtact acgacccaaa tgtttggcga aaatacgcaa
     2641 tcaccagtgg aaatggtcaa ctggaactgg atgctcccag gattcgttaa ttacaaggaa
     2701 ggcgtggacg gattaaaaac tggtacgact gagcttgcag gtgcttgttt cgttggtacg
     2761 atcactaaaa acgatcaacg gatcatcact gtcgtattga atgcggacgg ccatgcggaa
     2821 aacccgagtg cccgcttcaa tgaaacagac cgtttgatgg actactgcta tgacaattgg
     2881 tcagaaaaag agctcggaaa agctaacgca agtattccta gtctaaaaac cattgatgta
     2941 aaagacggaa aagaatcaac agttcctgtt gtgcttaaaa gccctgtaaa agtatgggta
     3001 cgaaacgata tggatactgg caaattaacg atcacgccaa cacttgacaa gaagcaagtc
     3061 actgacaatg agatccaagc acctatggat aaaggtacaa aaatcggtac cgctacgatc
     3121 accttggcag acgataaatt aggctattta gaagacaaca atgcaccatc aacagagatc
     3181 attacggata aaacagtgga aaaagcgaat atattcgtga ttggctggcg acgcgtcgca
     3241 gaatttttcg gcaacttgtt ttaattgaac aataagaaaa acatctttcc atcggaaaga
     3301 tgtttttttg ctttttctag tttaaataac agcttttttc atctattttc gttatactgt
     3361 ataagaaaaa ggagttgaat ttatgaatct aaccagagat ctgatctttt tagttctcta
     3421 tcttattggg gtattctcta tcgtttattg gcgaaaacag aagaaggatc cttccttgtt
     3481 cttttacggc agtacgtgga catttacggt aatcttaact tattttgtcg tgttggaaat
     3541 tttttattcg accttttatt ttttgattcc attgttgttc tttagtatct ttgctttttc
     3601 ttatttcaca gaaaaacgaa ggctactgaa tggtctttta ttcaatgttt tactcatcag
     3661 tttcggcatc tacttgtttg tcttgctgta tgaaacgcaa aatatttttc ttggcggact
     3721 gatcgccttg atcacgattc cattgctact tgtcctttta tttggtattt atggattgat
     3781 tgtttttctt ttttggaacg gggtcacagt tttacgtaga gaatcgcatt cattagctaa
     3841 tcttttaacg ttgatactag ccatttttct tacactgttt cttgtttttg actttttctt
     3901 gttaaaatat ttgccgcaat ggataaatgc tttgtttttc tgcgttcccc tgatcttgat
     3961 ctatctattt atcgtattct acaacttttt gactgtctca ttcttgtatc agttcaaccg
     4021 gcctcgatac aatcaagact tcatcgttgt gttaggcgct ggattgatca atggagaaac
     4081 agtttctcct cttctagcca agcgaatcaa taaggcaatt gctttttatc gggctcaaag
     4141 ccgtgctaca ttaaatccgc ccatcttatt gatgtctggc gggcaaggag cagacgaaaa
     4201 agtaccagag gccattgcga tgaaacaata tgccatggaa caaggaatcc ctgaaagaga
     4261 cattcttgtc gaaaccaatt cgacaaccac tttggaaaac atgctctatt caaaggaaat
     4321 catggatcag caaatgaaag ggccataccg cgcgatcttt agttccaaca attaccacat
     4381 ttttagagcc ggtctttatg cgcggcaagc caaattgaaa gcaaatggta ttggtgcaaa
     4441 aacagctttc tactacttgc ctaatgcgtt tttaagagaa tacctcgcca tcttgatgat
     4501 gaacaaaaaa agacatatga tcatttgcgg attgcttttc gcaggctccg cattcttatc
     4561 gctgattact ttacttttct aaaaaaacaa ggtgtgacaa aacatatgtc acaccttgtt
     4621 tttttctttc attctcttaa ctattggcaa aaagagtatc ccttttttga ccgctacttg
     4681 ctgagcagaa taaataccgc tgttgcctga acacataaag ctgatcaagc agatcataaa
     4741 gaagtatact gccgcttgtg aaccgaacaa ttcgatcccc atcacaaagc aggcaatcgg
     4801 tgtatttgtc gcaccagaaa atactccgat aaagcccaat cctgcaagaa aaggaacaga
     4861 aacatggagc aaaggcgcta gtgctgcacc aagcgttgca ccaatttcaa acaatggtgt
     4921 cacttctccc ccttgatatc ccgcgcctaa agacaaaact gtgaagaata acttcccaat
     4981 aaaatcatag acatgtgcct gtccattaaa tgattcttga agtaacggaa gactcaatcc
     5041 gagataacgt tgtgtttgca aaatcaatgc tgctgccaca actacagctc cacctatcgt
     5101 atttcgacaa acaggatgag gaatccattg tgcatatcgc gcttttaaaa agacgatcga
     5161 acggctgaat acccaaccaa tcaaaccaaa agcaatccca gcaacaaaaa ctttgaagaa
     5221 cagcaacata ctccattcag gaatcacacc catttggtaa tgtagatgag aaacaccata
     5281 gctttctgtc atgaaatttg caaacagtgc agcaaagaag ctagggaaga tcgcttctgt
     5341 tcggactttc ccgattgcca acacttctaa tccaaataat gttccagcta aaggtgtgcc
     5401 aaatacagaa ctaaaacccg cactgattcc actgataatc aagatttccc gttctgattt
     5461 atccaatcgg aacaaacgtg ccgtattatc tgctaacgct ccccccattt ggactgctgt
     5521 tccttcacgg ccaacagatc ccccaaataa atgggtagtt atggttccaa acagtgtaag
     5581 tggaataagc cgcaacggga ttttttcttc tccaccattt ccttgatcga tcactaagtt
     5641 gtttccacga ctagaaagtc cgccgtgata cgcatagaga taagcaaaaa gcgcaccact
     5701 aactggtagt agacaaagga gccaaggatg tgatagtcgg atatctgtca caactgtcag
     5761 gctatttaag aaaaaagcag acaagctccc catgaatata ccgagaatcg ctgtgatcac
     5821 cagccacttc agcatataca ctaaaatgcg tactggactc ggtaattgtt tttccataaa
     5881 aaattcctcc tcaataaaaa aacgcctact aaagaaaggg gctgtcccca gactttagta
     5941 ggcgtcatta gctatctttc gttagcattt aaaggcgaac accatcacct attcacttcc
     6001 attaaaagat agcatatttt tctgtaaaaa acaataaaat tatcgttctt tctttacacg
     6061 ttcagcaaat tcatagaaag gagccaattt cgttaactgt tgattacatt ggtcactatc
     6121 atgacaaata taattcccct ttttcgtata cgtgccatca gaacctgctt tcgttgtaga
     6181 aagaaaaagc gaaacattcg atgttttctg acagattgca cagattccct tatttatcgt
     6241 tggtgataaa gtaccgctaa tcccttttaa tttttcatct tcgtaataga ccatgaattt
     6301 tttctgcgta cctggatcat tccagccgat gtacgtatgt tcttgcaagt cgatttcatt
     6361 ccaagctggt tgtttgagtt ttttcacttt tcggaataat ttctcgattt gtggtttgct
     6421 aggctgcata aacggctgaa cgatttgctt cagctcctcc aatgctcttt ctgcttgcgc
     6481 ttttgtcagt tttacgtcta aaactgtcgt taaaaatgta tcgattctgg cagttcgtga
     6541 tctaatatgt ctcgaatctt ttcagctgct agtgcctgaa ctgtttgtac cgtttttgga
     6601 tcattgacag atgagtacgc atttaataac tgttctactt ctcttattat aaaaaaatat
     6661 tggtatggtt ttattgtttg ttccatgatt ttaaagtcct ttcttctaac caaagtgcat
     6721 actttgtaga aaaaagtgat cttctgcaaa gtatgtgtta cctgaagatc ccggactctt
     6781 ttaaataaga tacgttttgc atcttatcat cacccccttt aatgcttatt attctatgta
     6841 catgattcat tcgataaata aaagtaaacc gtatcttgaa cagatagtca actgcaaaca
     6901 gaaaaaagga ctgtgacaaa agtcgtgtca aagtcccttg tttcgaatga atggtgttca
     6961 aaagctggca tctgcggtaa taagccgaaa cttcacaaaa atttgagaag caattttcgt
     7021 aaattccttc ttatttcttg aagctgacca cttctgtcac aacctcaata tggtctatta
     7081 ttggttttat ctattaaatc gaggattctt tcccaaagta acgttgccat ctcttacgca
     7141 tacttgctga cataggctga gcggcttctt tgactgcgca gtatttgtct acgaatgtta
     7201 cgatatatcc ttctttgtac ttaggcggtg caatcgttgc gccccacatg tgcttcaaaa
     7261 tgatatctcg ctcaagatcc gacaattcgg tgatcttttc tgcattcttt acagcaattc
     7321 ttggatggat atacgcatgt gtgccttcat caaatttcgt cgtacgccaa tcatagtaga
     7381 acagatcatg caaaagtcca gcacgcgctg ttgctttagc attgccaccc catttttttg
     7441 cgagcagata actatagtaa gaaacactga tggaatgctc caaacgagtg gaatgaacat
     7501 gctgtgtgta attcgctaat tttttcactg cttctgtttc taaaagatct tctacataag
     7561 acatatattc ttcgtcttca cgccattttt ctgtcataat tatcgatctg ccaccttttt
     7621 gaatttcatt ttcttctgaa attgatctaa gtataccacc agtgttcgga ttattctagt
     7681 cgccttggat aaaatagcca aagcttaata gttccttaat cataaagaaa aatgataaag
     7741 aacaatccct gccgcaaccc caacattcaa cgattccgca tttcctttca tcgggatata
     7801 tacgttctga tcagtcaaag acaagatttc ctgacttacc ccctgacctt cgttccccat
     7861 gattattgcg aagttatctg atttttctac tttgtctaat gcgtgtgctt cttcatttaa
     7921 ttctgtgcca tacactgtaa accctttgtc tttcattaaa gaaatcactt ctgtcaaatc
     7981 tttttgcaac acaggcaaat gataattgct tccctgcatg ctgcgcagca cctttgtact
     8041 ataaatatct gccgttcctt tgcctaataa aacaccagcc atatcaaatg catctgccgt
     8101 acggatcatc gtccctacat ttccaggatc ttgtacatta tctaacaaca gccatcctcc
     8161 tgaaaaatct ggcgtgcctt cagccgttaa cgccatcact gctaagattc cttgtggtgt
     8221 aggtagatcc gatacagcat ccatgacctc atccgaaact agaaaagtag aaatctcttt
     8281 gtttgcggcc agccagtctc cccattcttt catgcctcgc tgtgtcacaa aaagttcttt
     8341 tatttctgca tcagacgacg cagcttcttc aatcagatgg aacccttcca aaagatattc
     8401 ttccttttct tcccgatatt tctttttatg tagttttttt aattctttga ttcttggatt
     8461 ttttgctgat tggatctctt tcacattcct cacctcccca tgattatatc atggaatagg
     8521 tgcttgagct tgtttcatcc taaaaaaatc ggtatcatta atttataaaa taggaaagaa
     8581 aggtgacgaa catgcgcaaa gttaaaatga acgtccaagg acgcgtccaa ggcgtaggat
     8641 ttcgctacat gaccaagatg gtcgctgatc aactaggcgt gactggaacg gtaaaaaacg
     8701 aagatgacgg ctcagtcacg atcgaagcaa tcggcaatga cgacatcatc cagaaattta
     8761 ttgaagaagt caagaaatct ccgagcccaa gtggacgagt gcagtatgtg gatgtccaag
     8821 aagacccgat gattgaggaa cgaaagaaat ttgatgtggt gggataaggt tgtgaaaaag
     8881 ctggtctgca tcaagcatta aggacaggtt gtgaaaaata gctctccata tttttcaaca
     8941 ggctggactt aatgccgtag atgcaagctt ttgaacaccg tcgataaggt tattcttgag
     9001 gcgctttgcc tcaaaaatta aggacagcag tgaaaaagct gatctatcac ttgcagtgag
     9061 ttcatttgac tccgcttttt cgggcagaaa aaaagttttt agtttattaa agtaagaaag
     9121 aaacacttgg aatcattcta ctttttccta gtatttcttt cttgattttt tgtctttttt
     9181 tcaaaatttt tcgctacgta aaagaacgaa tatttatatg aaatttctat gaagttgatt
     9241 gaattccatg cgctttttta gtatagttaa gtttgtgtaa aaattataga agaggaagta
     9301 ttctatgaat aaatggaaaa actggttatt gggatccgga cttgtcagta tcctgctctt
     9361 tttatctggc tgtgtgcgga tggattcaaa cggaaaccct gacacttcgg gaatcgttta
     9421 tcgagtactc gtccatccta tgggccaagc gattacatat ctagttgaaa atttcaattg
     9481 gtcttatggc tgggcagtta ttgcaatgac tgtgatcgtc cgtatcatca tcttgccact
     9541 aggtattagt caatcgaaaa aaacaatgat ccaatctgaa aaaatgcaag cgttaaaacc
     9601 acaagttgaa gcagcccaac aaaaattgaa agctgcaaca acacgtgaag aacaaatggc
     9661 agcacaagca gaaatgcagc aagtataccg cgaaaacgga ttgagtatga ccggcggaat
     9721 cggctgtctg cctctattga tccaaatgcc gatcttctct gccttgtact ttacggcacg
     9781 ttatacagaa ggtatccggg aatcatcttt ctacggcatc gatttaggct caccaagtat
     9841 ggtacttgtt gccattgcag gggtcgctta tctgcttcaa ggatacatct caacgatcgg
     9901 tattcctgaa gaacagaaaa agacgatgcg tacgatgctg atcgtctctc ctgctatgat
     9961 cgtcttcatg tcgatcagtg cgccagccgg tgttacactt tactgggtag tcggtggtgt
    10021 gttcagctgt ctgcaaacat tcatcacaaa cgttatgatg aaaccacgtt tgaaagcaca
    10081 agtggcagaa gagttgaaac aaaacccacc aaaacaagtc gtcactccaa gaattgatgt
    10141 gacagaatca gctaaaccag ctgacagcaa acaagcaacc aaacaaatca agcaacccaa
    10201 aaacagtaca aacaataaag gccgcaacgc aggaaagcag cagaagcgga agtagatagt
    10261 aaataagagt gtaataaaaa ttcattaaca gttttagcaa ttaaaaatat cgaccaagat
    10321 ttttgaaaaa gaaatctggt cgatattttt atttaagtga atgtttaatt tgaatcatcg
    10381 attgatcgct atctaattgc agcagcatct ttttatgaag aaacaacttt ttcttatcta
    10441 tcaacgtaga caggtgttgc tgtttcttct aatagtgtac ttaattcctt aatattttgt
    10501 tttccttctt ctaacatcca actactcgct ttttcttcgc cttcggtaac aaaaggcgcc
    10561 actcccttcg cccatgttgc tcgcccacat aagacaccat tctccataaa ggacaacgcg
    10621 gagataccac ccatctaaat aacatccata aacctttcta gtagaagaat agtgatagta
    10681 tccatccctt tttctactgg gaatacaatt acacgattca aagcttgata cacaacattt
    10741 cccttcttat aaaccgctat tgtaaccgaa ttgttttgaa tcaccttttt gggatagaaa
    10801 ctggctcctt tccgcaactt cttaaatgtt ggtttctttt atactttctt tgtagcaact
    10861 acaaatttct tcaaatctct aaatgtttgc ataacaaccg cctttctgac ttttactcag
    10921 gatggtattt aaaaatcagc aaaataaggc ttctctccat cttttttatt tcctagctgc
    10981 tttaaagaca ctcttgctgt tttttaatct ctatcaaggg ggctctgaat ctattttgat
    11041 gaacttatct tattaatttc tcaaatgagc tgtcagacac aactgtattt tttcaatctc
    11101 ataccggaca actatgccag cttaaaaaaa tttcctctgg caaaacttct ttattgaaaa
    11161 atgacaatga aaaaagacca ttctttttgt tgaatgctca ttgtgtaaac atatttcctg
    11221 cgactgctct tcgaaccatt ctcagtgcaa tccctaattt atctattaaa tcttctttta
    11281 gttcctcatc tttcatattc aaaatttcct ttgtatgtta cacaagtttg ttctctaaat
    11341 a
//
LOCUS       DACDEG010000077        11303 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.081, whole genome shotgun sequence.
ACCESSION   DACDEG010000077 DACDEG010000000
VERSION     DACDEG010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.081"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            138..1373
                     /locus_tag="IV142_002086"
     CDS             138..1373
                     /locus_tag="IV142_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP8485733.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(2372..2632)
                     /locus_tag="IV142_002087"
     CDS             complement(2372..2632)
                     /locus_tag="IV142_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP8485734.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2659..3960)
                     /locus_tag="IV142_002088"
     CDS             complement(2659..3960)
                     /locus_tag="IV142_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8485735.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(4252..4710)
                     /locus_tag="IV142_002089"
     CDS             complement(4252..4710)
                     /locus_tag="IV142_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP8485736.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(4723..6048)
                     /locus_tag="IV142_002090"
     CDS             complement(4723..6048)
                     /locus_tag="IV142_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP8485737.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(6065..7477)
                     /locus_tag="IV142_002091"
     CDS             complement(6065..7477)
                     /locus_tag="IV142_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8485738.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(7494..8258)
                     /locus_tag="IV142_002092"
     CDS             complement(7494..8258)
                     /locus_tag="IV142_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP8485739.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(8362..9237)
                     /locus_tag="IV142_002093"
     CDS             complement(8362..9237)
                     /locus_tag="IV142_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8485740.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(9492..9576)
                     /locus_tag="IV142_002094"
     tRNA            complement(9492..9576)
                     /locus_tag="IV142_002094"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9540..9542),aa:Leu,seq:caa)
     gene            complement(9594..9665)
                     /locus_tag="IV142_002095"
     tRNA            complement(9594..9665)
                     /locus_tag="IV142_002095"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9631..9633),aa:Gln,seq:ttg)
     gene            complement(9675..9747)
                     /locus_tag="IV142_002096"
     tRNA            complement(9675..9747)
                     /locus_tag="IV142_002096"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9712..9714),aa:His,seq:gtg)
     gene            complement(9809..9893)
                     /locus_tag="IV142_002097"
     tRNA            complement(9809..9893)
                     /locus_tag="IV142_002097"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9857..9859),aa:Leu,seq:caa)
     gene            complement(9914..9984)
                     /locus_tag="IV142_002098"
     tRNA            complement(9914..9984)
                     /locus_tag="IV142_002098"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9950..9952),aa:Cys,seq:gca)
     gene            complement(10030..10101)
                     /locus_tag="IV142_002099"
     tRNA            complement(10030..10101)
                     /locus_tag="IV142_002099"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10067..10069),aa:Gln,seq:ttg)
     gene            complement(10113..10185)
                     /locus_tag="IV142_002100"
     tRNA            complement(10113..10185)
                     /locus_tag="IV142_002100"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10150..10152),aa:His,seq:gtg)
     gene            complement(10188..10261)
                     /locus_tag="IV142_002101"
     tRNA            complement(10188..10261)
                     /locus_tag="IV142_002101"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10227..10229),aa:Trp,seq:cca)
     gene            complement(10268..10348)
                     /locus_tag="IV142_002102"
     tRNA            complement(10268..10348)
                     /locus_tag="IV142_002102"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10312..10314),aa:Tyr,seq:gta)
     gene            complement(10350..10425)
                     /locus_tag="IV142_002103"
     tRNA            complement(10350..10425)
                     /locus_tag="IV142_002103"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10390..10392),aa:Phe,seq:gaa)
     gene            complement(10461..10533)
                     /locus_tag="IV142_002104"
     tRNA            complement(10461..10533)
                     /locus_tag="IV142_002104"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10498..10500),aa:Asp,seq:gtc)
     gene            complement(10543..10615)
                     /locus_tag="IV142_002105"
     tRNA            complement(10543..10615)
                     /locus_tag="IV142_002105"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10580..10582),aa:Val,seq:tac)
     gene            complement(10631..10702)
                     /locus_tag="IV142_002106"
     tRNA            complement(10631..10702)
                     /locus_tag="IV142_002106"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10667..10669),aa:Glu,seq:ttc)
     gene            complement(10717..10806)
                     /locus_tag="IV142_002107"
     tRNA            complement(10717..10806)
                     /locus_tag="IV142_002107"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10770..10772),aa:Ser,seq:gga)
     gene            complement(10846..10919)
                     /locus_tag="IV142_002108"
     tRNA            complement(10846..10919)
                     /locus_tag="IV142_002108"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10883..10885),aa:Asn,seq:gtt)
     gene            complement(10929..11044)
                     /gene="rrf"
                     /locus_tag="IV142_002109"
     rRNA            complement(10929..11044)
                     /gene="rrf"
                     /locus_tag="IV142_002109"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(11138..>11303)
                     /locus_tag="IV142_002110"
     rRNA            complement(11138..>11303)
                     /locus_tag="IV142_002110"
                     /product="23S ribosomal RNA"
BASE COUNT         3575 a         2453 c         1834 g         3441 t
ORIGIN      
        1 aaatttaaat ttagaagttg tgaaaacagc gagagacagt ttaatattca aaatatacat
       61 aggctactcg aacgctgtgt tttttagtat actagtaaga aacgaaggga tagactcatt
      121 ccaataagga ggatttcatg aagacaattc tttttgctcc tgcagtattc aatttagctg
      181 agacaacacg aatgattgag gttgccaaag ctatgaaaga ccgttattac tgtgtattct
      241 ttggcttttc taaaactttt gctcctttga tcacagaaag tggctttgag tatcatttac
      301 tagcgccgat ccttacgaaa aaacaagaag aacaaatcat aaaatttgac cagttgcgta
      361 cacttaaaaa cccttttacc aaagaaatgg ttgcctctcg tatcactagt gaacagagtc
      421 tgattgctaa gtttgagcct cgactgattg tgacaggttc gaatgtgaca atttttttat
      481 cggcccgcaa taaaaagatt cctttagtat atgttaaacc ctatgctttg agtgaacctt
      541 tttttcaatc agagcatgcg ctgatgcccc acgagttacg tttcttcggc cctttaagca
      601 atcccctttg gcactggaca aaaaagcgta tagtaaatac ccgatggatc cccaatgcct
      661 tcaaacacgc tgcacaaaaa gaaatgcttc ctttaccaaa aacaagttta caaatgatgg
      721 atgctgatct aaatctcatc acaacgccta aactattttt ggaagaagaa cagctgccgg
      781 aaaattatcg aatcgttggt ccaatttttg ctaagctcag tacttccttg cctgaagaag
      841 tgctaacttt tatacagcga aagcaacaag aaagaaaacg tattatctat ttcgcaatgg
      901 gtagttcagg caatccgcgt atgttacgca aaattttgtt cttcttacaa aaacaaccgg
      961 atctagcggt tattgcacct attgactatc tgcttgatga cattcaatct gactcagaag
     1021 atcttttaat aacagaatat ctgtctagtc atcttttgca tgaattcatt gatttttctt
     1081 ttatccacgg cggggaaggg accgtccaaa ctgcctgtga atccggcaaa ccgtttatcg
     1141 gtgtggggat gcaatttgag caaagctgta atattgacta ttgtgtaaaa tacggaaacg
     1201 caatcgccct cacaaaacca gtgactgaaa gaaaactgca gattgctctg cgcgaagttt
     1261 cttcacccaa gattcgtcag caggcacgca cgctgcaagc cgtttttcca ataaatggtc
     1321 cacaacaagc aattcaagaa atcgaaacct tcctgagcaa ccacccaata tagtttcaag
     1381 tagaaaaagc ttttttaata gagactcatc agtaatttcg ctattcgaga cccagacagt
     1441 gaaaatacaa aaaaagtaaa tagtggattg aaaagttaga gttacttaaa ttacggtgtc
     1501 taaaaaacac aaaatcatcc ttaaaataaa agatctgacg ctatccaaat gagcataggt
     1561 agcgtctgcc atttattctt agtcaaattc tttgacacct ttctctggtt ggatcatctc
     1621 aacgtttctt caaaagaaag accataataa ttaggcaaat acattttctt aaaatgtgtc
     1681 atatagatct agaagcatcg ctttgtagag atttgtgccg ctagtcccct caacaattac
     1741 taaatcaaat gtaggattta ccactttatc tttgataatc aacctccctc ttctgttgag
     1801 acaatgatct cgcaatccga tgcatactcc tatataaact gccagcaatc cccacatatt
     1861 gagtagatca tgtcagaatg ttctatgtcg tcagcaacga tacactaaaa gtcacgttca
     1921 ccatgactga ttgctattcc cagcacaatt atttctatac acgtttcatg atttgaataa
     1981 acactcacat catacatatt accattcata caccatactg cagcccctac tatatggttg
     2041 taagctccca aatctatatt tgacttgatc actttggtag tagatctact aattctaaat
     2101 ccattgctga taaaatttcc attttttcct ccttctttct gccaaaacag ttaaaacaat
     2161 aataatcttt atgtaaaaaa gggctgtgac aaaagccttg tcacagtccc atattccgaa
     2221 taaacggtgg tcaaaagctt gcatctgcgg taataagttc aaccaaacga aaaataagcc
     2281 aacatttcac aaaaatttaa gaagcaattt tcgtaaattt cttcttattt cttgaagcta
     2341 accacttctg ttacaaccac ttttagaaca tttaaagctt cgatgtgaca aaccatctat
     2401 gctgctcgat cataaaattg atagcaaaaa ttccacaaag tacatatgtc ccccatgaaa
     2461 acaaaacatt catatgcggt acaatgatct ttggaagaat cacgatcaaa aatgatgcca
     2521 gtgcccaaat ccatcgattg atttgaatat gatatgtagc tagcatattc aatataaggt
     2581 gactagccgt taataaaaag ataaaaacaa aaaataaaat cagccaatac attgtttccc
     2641 accttctaaa aaccaaactt agacagtctg gacttcgttt tctccacgac tttgttcatc
     2701 tgacagcatc tttttatcat aagcttttac aaatggataa tagataataa atgctatcaa
     2761 tgtgttgatc atgatcaaga taacaacacg ccagtcattt cctgctgaaa gataaccgcc
     2821 gattggagct ggtaatgtcc atgttggcaa aacagtaaat ctattgacca tttctaattt
     2881 gactgcaaaa taactgataa tagtcaaaac taaaggaact aagttgaacg ggatgaacat
     2941 gtaaggattc aacatcatag gagctccaaa aattatgggc tcattgatat tgaagatacc
     3001 aggaataata gaaaaacgac caacctcttt caagaatttt gatttacaga agaatataaa
     3061 caaaacagat accacgatcg ttgctcctgc accaccaatt gttaccgtcc attgataaaa
     3121 ctgttcagga gcgatatagg gtaattgggt ggcagctgta ccatctgcca atgcctttgc
     3181 attctcatcc aacatgatca gccacattgg acgaacaaca gcccctacaa tactcatgcc
     3241 atggatacca aatgaccaca acaaatggat caaaaacatt ggtagtaaaa caccaagcaa
     3301 attattccct aaaatattgg ttaaaggctc gaataacgat aataaggcag cgtttacatc
     3361 aaagcccgcg acgcttcgaa tcagccaagt aattgttaaa atgactgcac ctggaatcaa
     3421 tgcctcaaaa ctacgggcaa cagcaggtgg cacttgttct ggcattttga tagtaatatt
     3481 tcgattttta aagaaacgat atacttccac tgcaaaaatc atagataaga tccccgcaaa
     3541 catcccagat gctcccaaag aactcatagg taagacccag ccaagaggtg cctcttcagg
     3601 agttaaaacg tttacaggaa cttgtaacat aaagaacgtt cctaaagata atataccacc
     3661 tgtcacacta tctaatccat atgatttagc tagatccgaa cccataacaa aagttgcata
     3721 aatagccatc aatcccatcg tcatcctaaa gggtaaaaca atcgttgccg aataaggggc
     3781 aataaattca ttccatccag gaaacggtaa attgataaag accataaaaa atgtcccaat
     3841 caaaatcaat gacaatgttg aaacaacccc attacgaatt gctaataaat gacgctgtgt
     3901 accaattttt gccattggtg ggacaagttt ctcttcgacc catttcatca gcttttccac
     3961 taaaaccact ccttttcaat aaaatatgaa ccattcattg catgacaacg caaagaatgt
     4021 tcataactaa ttatataatt aagaaatttt atttcaatat attctcttaa ataatcaaat
     4081 ttatttttaa gaatcctgaa aataagtttt ctaaatcata ggagcaacaa aaaaattcga
     4141 taattaacaa aaatcaaaat gattaaatct agtgattgaa acatatcatt tcaaaaaaat
     4201 tttgtaaatt cacccataaa tatcacccta tttaactttt ttcatcgtac actaaaattc
     4261 aaaacaacta tctttaatct ttacttcttg gttttgtttt aataaatttc cttcaaaaat
     4321 gcctggagaa actacaatga tcaccgtatc tttttgtgct tgctccaact tcttcaaatc
     4381 tataatagct aacagtgtcc cttcttttac ctcctggtta ctttcaaccc gtatctgaaa
     4441 cggacttcct tcgagttcaa ctgtatctaa acccatatgg atcagtattt ttctgccaga
     4501 ttcactttcg atcgttatgg catgtcgggt aggaaatata ttatcgatta ttcctttaac
     4561 tggcgcatgg atatatccat catgattcgt tatagcgtat ccaactccta tcatttttga
     4621 agaaaagacc tcgtctttta ctgctgttaa tggaataatt tgaccactag ctacagcttt
     4681 tagaatgttt cttgttttaa agaaatgcac tttttatcac ctctacttta atgctggcca
     4741 atattttttg tttactttga tcatttcatc tagtattttt tttgcgactc tggcatttgg
     4801 tacggtttga ttcaatgtaa atgcttgtaa agctttttga taagaacctt caaagtaagc
     4861 atctactagt aacttctcta ctgcaacttg tgcctccatc aatcctttat gaaaatcact
     4921 gattggtttt cttagtgtaa ttggctccac accagttgca ccaacatatg ccggtatttc
     4981 aacaactgca tctgaccgta gattcggaat cgccccttga ttaggaacga ttagcataaa
     5041 gcgacgacga tcatcattca acaaggaaat agctatatcg acgatatatt gtccatgctc
     5101 cccaaaatta aagttcaata cttcttccac aataccgcgg cgtattttat cagccatttc
     5161 tttggtattt ttctctcgtc catccatgac catattagca cgtgtatact ctttgtcagt
     5221 atgctcaacg accatatcag gatataagta atactctaag taattattcg ggatattact
     5281 tggaaaattt ttcgtgatga ctgacatcat ttgaaaggtt ttttgccaag ttttatctcc
     5341 tatattaaaa tcggcgactt gcatttcttt tgttattagt ttttcaataa tctctggcat
     5401 aatttctcgt tgcaattctt tatcataaat cgatgtatac cagccaaagt ggtttaaacc
     5461 ataatacgta acgatccaat tcttacgatc ataaccgtaa ttcactgcaa tcgtttcttc
     5521 aattgaaata gtcatatcac aggcattaat catttttaca ttcggatatt tacgacgaac
     5581 agcttcagaa acgatcgact ctggatttgt ataattcaaa atccatgctt tgggggcata
     5641 gtcttgtata tgatcgacaa tttccaataa acctttgatt gagcgcatcc cataagcaaa
     5701 tcctccgagt ccacacgttt cttgtcctac taatccatgt ttaagtggaa tcttttcgtc
     5761 cttttctcgc atctccaatc ctccaacacg aatttgtgag aaaacaaaat cgcatcctgt
     5821 aaaagcaagc tttggatcat ccgttgcacg aatgcgaata tttgaaaaac cttttctttt
     5881 cagcataaat ttaacaatca agtacatatc catatttctt tcttcatcaa tatcatataa
     5941 acgtatttca gataatggca accgtttttc attgttcaaa atggcttgaa cgattcctgg
     6001 tgtatacgta ctcccgcctc cagctatagt gataatctgt tttttcatca tcatcttttc
     6061 ctttctattc ttctaataat ttattgaatt ctgccataac ataagtcact tctaagccga
     6121 tgataatttg ataatttttt tcagttggtt taatgatccc gcttatccct aattgcttga
     6181 ttcgctgttc ctcgaccatg gtgggatcca ctacctctac ccgtaggcgt gtattacaac
     6241 tatagacatc caggatattg gcttttcctc caaacgcttg tagcattttt gttgccatat
     6301 attcataatt tttacttgct aaccctaact ctgtttcttc taaggttact tcattgccaa
     6361 actctgcctt agcttcacgt cctaatgtct gaatgtttct cttattgatc aaataataaa
     6421 aagtaacgta atacaaggca aaaaagaaaa ttccgattgg aagaatcaaa atagcattgg
     6481 tagcgattcg aaaattgatg agataatcaa caatacatgc tccgaatgta aaaccaattc
     6541 gtatattgaa taagtaacag acaatccctg ctaaaccggc aaaaagtgca tggatcacgt
     6601 acaacatagg agaaagaaac atgaaactaa attcaacagg ctcagtgata tttgcaataa
     6661 atgaagttcc tgcaccgctt cccatgattc ctttgaccat ctctttattt tctttataag
     6721 ctgctttata gatagctaga catatagctg gtataccaaa catcattact acaaaaaaaa
     6781 ctgataaaaa cagtccagcc tgaggatctc cgttgataaa gcgtgtcatc tctcctctga
     6841 aaacttcctt gctaggagat gtatagcttc ctaaatcgta ataaatatac gtattcaaca
     6901 catggtgcag tccaaagggg atcagcaaac gattcaaaaa cataaatatc cccacaccta
     6961 atgctcccat atcaacgagc agttttgcaa aattatcaat cccaatttgg atgagcggcc
     7021 agataaagcc caaaaaaaca gctgcgattg tttgtagaat catcaccata gtcaatggga
     7081 atttttcgcc cgcaaaataa gaaaaaacta ctggtaattt ttgttcttta aagcgattat
     7141 acgtccatgc cgctactaaa ccggaaataa ttccaccaaa aatccccata ttcacttctg
     7201 ggtccataat ttctaagcct tttttcaagg tagcaatcgc taaaaaacca gtaagtgcag
     7261 gtattccttt gtccttattt ttgacaaaac cgattgcaac tcctatagca aataagatat
     7321 ccaagtttcc aaaaacggca tttccagctg caccaattac tgggatgttc agcatatcgt
     7381 cagctgataa acgcatgatc aatcccgcta atggcatcgc tgcaactgga agtagcatcg
     7441 ctcttccaag cttctgcaca tttgatttaa cattcatttt tattcctcct ctattaaata
     7501 tcctcatatg ttactaactc aatcaaagga tcatttttca ctttttgagc aaatgctgaa
     7561 tgaattaaaa attcgacttg gtgggtacga ggatacacaa aacttgaacc atttaacaat
     7621 tcctgatcca aatacgctgc gtgacacatg acttcgatcg ttttatcttc tctcaatatc
     7681 atgagtaaac ttgccaaata ttcttccaca ctcgtattga tttgtcgttt tttttcatca
     7741 gtttctccta cgacatcgaa acaaggctca aaataatcga catgacgaac ttcatctttt
     7801 cgttcaaagt tgctccgaac cggcaaatca tatttttttg ctaatgtaac aactacctcc
     7861 tgattttcat ccaaagtatg agtatgatga tgactgtcta aatgagttgg tatgatcccc
     7921 gcatgataaa ttttttgaat ctgagcattc cattcctgat acagttgatc tttgtcaatg
     7981 ttaaatggat gctgataata agataaggat ctgaactctc cgctttcgat aagagtatcg
     8041 acagttttga ggagtggttt gccacacgtc aatgttaaat ggactcctac acctagccta
     8101 ggcatttcct ttcttagttt cacagcatgc tcgaaacccg gcatatttgc catcaatgta
     8161 gttgatgtca aaattcctcg ctgataagca tccataatcc cataattcac accatggcta
     8221 tagccaaaat catccgaatt aaaaatcact tttcccattt cattctcctt cttttttcta
     8281 ctaaaatagg cgcaaccaaa taatcatagt ccatttacta tgtgtcattt ggttgcgcct
     8341 atagatagtt acgcccttat tttaatagat tcttgctaga tgaattgcca aatagatgat
     8401 ttcattttct tgaatggcaa gttgaaattg ctgttcgatg atatctttaa tctttagtgc
     8461 tgtacaatac gctttgctat gtttaaatgt ttcagtaaaa aaaccaatat tttctactgg
     8521 cgtttcttcc tgataaatca agcgtttaac caggaatttg atatgaacca ttaagcgatg
     8581 ataagtcagt gattctttat cataaacgtt tgaataggat ttttctagat gggacaaaat
     8641 cgtggtcgtc atttctacta tttttaagct ctttaaacta ttaggctctc ctagctcagc
     8701 attaatgata tgcatagtga tgaatcctac ttcatttgac cctaactcgg tttgatatcg
     8761 atgattgatt cgctcgacca cttctactgc tatatcatat tcatttggat aaaggaatct
     8821 catttctggc aacataattg ctgtgacatc tctatcttct ctttttcgtt gaatagcaaa
     8881 ataaatatga tctgtcaatg taatatagat atttgaatct aatggagagg agatcctagc
     8941 tgttatcagt tcaatcactt cttccgcaaa atccataaat tcgattggaa tctgcgagac
     9001 tatctcactg aatcgtttga tcttttcttt agaatctaat acaaataatt tgtcagcatc
     9061 ctcaacatca aatggcttac tcactttagc agaaaagcca atccctttcc ccatcgcaat
     9121 tttttctatt ccatcgtcat cgatcaatac agcattatga ttcagctttt tcatcacata
     9181 caccgttaat tcacctcctc tatatgataa aacaaaacaa cttgcagact ccttcataca
     9241 tatttttgtc tccaaacaat ttttaaagac aaaaaacgac ctaagaaaga accctttctt
     9301 aggtcgtgcc taattgaata gtagcaccct acataattta caaaaatagt ttaacacata
     9361 ataaaagcgt tatcaatatc ttttttactc gttttatgat aatacttgta tcgaattgat
     9421 ttggaggtaa ttacataaaa acaaaaaaaa tatagcaact ctctataaga aagttgctat
     9481 attgataatt ataccggcgg ccggggtcga accggcacgt cctcaacgga cactggattt
     9541 tgagtccagc gcgtctgcca attccgccac gccggcaaat ttaaaaataa taactggggt
     9601 agctggattc gaaccaacgc atgagggagt caaagtccct tgccttaccg cttggctata
     9661 ccccaagaat aaagggcgaa tgatgggaat cgaacccacg aatgccggag ccacaatccg
     9721 gtgcgttaac cacttcgcca cattcgccat cataaaagat gttgtttaat ttctcaataa
     9781 aaaactaaga tgaagacatc ttagtcaata ccggcggccg gggtcgaacc ggcacgccct
     9841 caacgggcac tggattttga gtccagcgcg tctgccaatt ccgccacgcc ggcaagttga
     9901 tttattagta ctaaggcggt aaccggattt gaaccggtga tgaaggtttt gcagacctct
     9961 gccttaccac ttggctatac cgccattata ctattatcaa tctataaaat aaagaataaa
    10021 ttatagaaac tggggtagct ggattcgaac caacgcatga gggagtcaaa gtcccttgcc
    10081 ttaccgcttg gctatacccc aagaataaaa agggcgaatg atgggaatcg aacccacgaa
    10141 tgccggagcc acaatccggt gcgttaacca cttcgccaca ttcgccatgg caggggcagc
    10201 aggaattgaa cccacactaa cggttttgga gaccgctgtt ctacctttaa actatgcccc
    10261 tataaaatgg aggagagtgg attcgaacca ctgaacccta aggaacggat ttacagtccg
    10321 tcgcgtttag ccacttcgct actcctccat ggtggcgcgg gacagaatcg aactgccgac
    10381 acacggagct tcaatccgtt gctctaccaa ctgagctacc gcgccatatt ttaaaattat
    10441 taattgaata acaagaacga cggttccgac gggaatcgaa cccgcgatct cctgcgtgac
    10501 aggcaggcat gttaacccct acaccacgga accagttcat tatggaggtt aacgggatcg
    10561 aaccgctgac cctctgcttg taaggcagat gctctcccag ctgagctaaa cctccaacat
    10621 tcaattaaaa tgacccgtac gggattcgaa cccgtgttac cgccgtgaaa gggcggtgtc
    10681 ttaaccgctt gaccaacggg ccaggtaaat ttgctacgga gagtaaggga ttcgaaccct
    10741 tgagacagtt tggcaccatc tacatgattt ccaatcatgc tccttcggcc tctcggacaa
    10801 ctctccagag tgaaagaagc tcaatactct ttctcaaaat gagaactccg gcagtaggac
    10861 tcgaacctac gacatcatga ttaacagtca tgcgctacta ccaactgagc tatgccggaa
    10921 taacatcagc gtggcgacgt cctactctca caaggggcaa cccctcacta caatcggcgc
    10981 taagaagctt aacttctgtg ttcggcatgg ttacaggtgt atccttctcg ctatcgccac
    11041 cacactgtgg tgttatcttt tattgagtaa attttgttca ctcaaaactg gatttgaagt
    11101 cacataagtt tttttccgag tacttttctt ttaacctatt ggttaagtcc tcgatcgatt
    11161 agtatcagtc cgctccatac atcactgtac ttccactcct gacctatcta cctgatcatc
    11221 tctcagggat cttactttct taaagaaatg ggaaatctca tcttgaggtg ggcttcacac
    11281 ttagatgctt tcagcgttta tcc
//
LOCUS       DACDEG010000078        11257 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.082, whole genome shotgun sequence.
ACCESSION   DACDEG010000078 DACDEG010000000
VERSION     DACDEG010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.082"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IV142_002111"
     CDS             6..305
                     /locus_tag="IV142_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8485741.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IV142_002112"
     CDS             378..1739
                     /locus_tag="IV142_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP8485742.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IV142_002113"
     CDS             1761..2021
                     /locus_tag="IV142_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485743.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IV142_002114"
     CDS             2035..2787
                     /locus_tag="IV142_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP8485744.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IV142_002115"
     CDS             complement(2881..3486)
                     /locus_tag="IV142_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8485745.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IV142_002116"
     CDS             complement(3539..4513)
                     /locus_tag="IV142_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP8485746.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IV142_002117"
     CDS             complement(4891..5670)
                     /locus_tag="IV142_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8485747.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IV142_002118"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IV142_002118"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP8485748.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IV142_002119"
     CDS             7161..8558
                     /locus_tag="IV142_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8485749.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IV142_002120"
     CDS             8639..9079
                     /locus_tag="IV142_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8485750.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IV142_002121"
     CDS             9227..9553
                     /locus_tag="IV142_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8485751.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IV142_002122"
     CDS             9554..10303
                     /locus_tag="IV142_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485752.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IV142_002123"
     CDS             complement(10383..10838)
                     /locus_tag="IV142_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8485753.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3575 a         1963 c         2305 g         3414 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacag
//
LOCUS       DACDEG010000079        11170 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.083, whole genome shotgun sequence.
ACCESSION   DACDEG010000079 DACDEG010000000
VERSION     DACDEG010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.083"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            132..1391
                     /gene="lysA"
                     /locus_tag="IV142_002124"
     CDS             132..1391
                     /gene="lysA"
                     /locus_tag="IV142_002124"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP8485754.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1378..2178
                     /gene="dapF"
                     /locus_tag="IV142_002125"
     CDS             1378..2178
                     /gene="dapF"
                     /locus_tag="IV142_002125"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP8485755.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2181..3062
                     /locus_tag="IV142_002126"
     CDS             2181..3062
                     /locus_tag="IV142_002126"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP8485756.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3059..3775
                     /locus_tag="IV142_002127"
     CDS             3059..3775
                     /locus_tag="IV142_002127"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP8485757.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3781..4476
                     /gene="dapD"
                     /locus_tag="IV142_002128"
     CDS             3781..4476
                     /gene="dapD"
                     /locus_tag="IV142_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP8485758.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4492..5493
                     /locus_tag="IV142_002129"
     CDS             4492..5493
                     /locus_tag="IV142_002129"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP8485759.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5490..6851
                     /locus_tag="IV142_002130"
     CDS             5490..6851
                     /locus_tag="IV142_002130"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP8485760.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6811..7962
                     /locus_tag="IV142_002131"
     CDS             6811..7962
                     /locus_tag="IV142_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP8485761.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7937..9106
                     /locus_tag="IV142_002132"
     CDS             7937..9106
                     /locus_tag="IV142_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8485762.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9281..10660
                     /locus_tag="IV142_002133"
     CDS             9281..10660
                     /locus_tag="IV142_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8485763.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10941..11170
                     /locus_tag="IV142_002134"
                     /pseudo
     CDS             10941..11170
                     /locus_tag="IV142_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3723 a         1950 c         2498 g         2999 t
ORIGIN      
        1 tttaaaaaaa tatttcgaaa tatactagac aattcagaaa attttgttta atattattcc
       61 aataaaattc aaatctttat caaatgaaat tccaataaaa acggaaaaaa agtgaaaaga
      121 ggaatgaaaa aatgacagaa ttgataattg gcggagtcac agcaagcaca ttggcagaaa
      181 agtataagac acctctgtat gtgtatgatg aagaaaagat gagacaaacg atgctgtcat
      241 tcaagcgagg ctttgtatcg aaacgttttg atacaaaagt gctttacgcc tctaaagcat
      301 tccaaacgat tgagatgctg catttagttc gtgaatatgg attcgggcta gatgttgtca
      361 gtggtgggga aatctatacc gctttgcaag ctgattttcc tagagatact atctacttcc
      421 atggtaacaa caagacgcca gaggaactga tctatgcact tgaaaatgat ttgttgcata
      481 ttgttgccga taatttgatg gaagttgaat tattagcaga attaagccaa cgctatttaa
      541 aaaaaatctg tatcatgctt cggttgaatg tcggagtaga agcacatact catgaatata
      601 tcgtcactgc gcatattgat tcaaaattcg gcatgagcta tgaaagtaaa gagtgccaac
      661 aagcccttca gttgataaaa gaaagcagct atctagagtt agaaggattt catgctcata
      721 tcgggtcaca aatttttgat gttactgctt ggttagctga aatcgacaaa ttgacgaatt
      781 acttaaccga ttttgaccaa cctctttcct tgaatcttgg cggagggttt gggattcgtt
      841 atacacaaaa tgatcagccg atcccagtcg aagaggcact aaaaaaactg attgaatata
      901 cggaagaagc tttggaaaaa cgaaaactga ccattcgtca attactgatc gaaccgggta
      961 gaagtattgt tggagaagct ggtacaacac tatacacgat tggtttcatc aaaaagacac
     1021 cacataaaaa atattacttt gtagatggcg ggatgacaga taatatccgt ccagctctgt
     1081 atcaggcaga atacgattgt gatttagcag caaaactgtc ggaagaaaaa acagagaaag
     1141 tgacgattgc aggaaaaatg tgtgaatcag gcgatgtggt aattaaagaa gcttatttgc
     1201 cgaaagcaga atcgggtgat cttttagctg tttattccac gggagcttat ggatactcga
     1261 tgagcagcaa ctacaatcga gcgacacgac cagcagttgt ttttgtcaat aatggccaat
     1321 caagactgat tgttcgcaga caagcatttt ctgatctact aaggggggaa gtttcctatg
     1381 atccttcata aatataatgg ctgcggaaat gattttatct tattagatta cgcagaagac
     1441 acagattata gtcaattagc cgcttcttta tgcacaaaag aaaaatatga tacagacgga
     1501 ttgatcgccg taaaaatcga tccgttggaa atgatctatt acaacaaaga tggcagccgc
     1561 gcgccgatgt gcggaaatgg cattcgctgt tttgctcgat acgtaaatga tcagaacatg
     1621 atgaatgaac aggaattcaa cgtagagaca ttagcaggca caagaaaaat tacggtgttg
     1681 gatccctcac ctttttattg cagcgtagat atgggagtgc cagatgattc tccggaaaaa
     1741 atcaaagcta atcaaactta tccaataaaa aaccaggaaa tagagatcaa tggtgaaaaa
     1801 gtaacgatca ctagtttatt tttaggaacg attcataccg ttgtttttgt tgaaaatgcg
     1861 cgtgaggaag tctggagaaa aagaggtgaa ctgatctgtc atcatccttt gttcaaagaa
     1921 aaaaccaatg tcaactttgt agaagtagtc aatgaacaag aattgatcgt ccgtacctat
     1981 gaaagaggag taggttggac acttgcttgc ggtacaggtt gctgtgcctc ttatgtggtt
     2041 gctaaggaaa ctgggaaaat ttcagcagat caggtagttg ttcacttgga acaaggagac
     2101 ttgatgatct ctggtgagga tgcgatacaa atggctggcc cagcagttca tgaatggaca
     2161 agaatattgg aggaatgaag atgataaaag gttcgatcgt ggcgctgatc acgccgatga
     2221 atgaagaggg atcagtagat tatgctggtt tggaaaaatt gatccaattc catttagatg
     2281 aacaaacaga tggattatta gttttaggta caacaggaga atcttctacg ctcacacaat
     2341 ctgaggagga gcagatcctt caactgacag taaagaaagt ggctggccgt gtgccggtga
     2401 ttgcaggagc tggaacgaat aatacgaaag aaacgattga aaaagcgaag cattttgctt
     2461 ctttgggggc agacgcactt ttggtcatca ccccttacta caacaaaaca agtgatgcag
     2521 gactagctgc tcattttaca gcaattgcag aagcctcacc gattccgctt attttgtata
     2581 atgttccttc tagaacaggc atgtcgattc caatccatgt attagtaaat ttagcagaac
     2641 atcctaatat cattggtttg aaagaagcct caggagatat ggcttatgtg atggatgccg
     2701 ctcggttgat tggagaagaa ttcttccttt acagtggaaa tgatgatttg atcctaccag
     2761 taatgagtgt aggaggaagc ggtgtaataa gtgtatgggc gaatatccaa cctaaaatag
     2821 tccatgaatt agtaaaagat acgcaggacg gaagatggca gcaagcaaaa gaaaagcaat
     2881 taaatgcact tgaactgatc catgccttgt tcagtgagac caatcctatt cctgtcaaag
     2941 cagccatgtc attattagac ttaccttccg gacctctacg cctgccttta gtttctcttt
     3001 caaaagagaa aaagaaacaa ttagctcagc ttttgttaaa agaaaggacg gttgaaaaat
     3061 gaaaatcgga ctgattggca gtggacagat gggaaataga gtcgaagaag ttgcacaaga
     3121 aagaggagat acggtccttc ttttacggac ggcacctaaa gaagcaacta cgcttgcttt
     3181 tacggaactg ccagaactgc ttattgattt ttctcatcca gataatttag agaaaatcct
     3241 ttcgtatagc ttatcatatc aacttccgtt agtcattggc acgacaggtt atacacaatc
     3301 acagttccaa gaaataaaag aacatgccaa acatgtacct gtgatgtata gtgctaattt
     3361 ttcgttcggc atcatggtga tgaatcaact aatcaagcaa gcagctgcaa tgttgcaagg
     3421 ctggcagatc gaactgattg aaaaacatca tagtcgtaaa aaagatgcac catctggtac
     3481 tgcaaatatg ctgcttcaaa caatccaaga agtgcaaaag ctgcagcccg tatacaactg
     3541 gcaagggaaa aaaagagaag aaaaccaaat tggtgtacac agtattcgtg caggttcttt
     3601 acctggtgaa catgatgtat tgtttgctgc tactgatgaa atatttacga tcaaacatga
     3661 atctttttct aatcgaatct ttgctgcagg cgcagtagaa gcagcggact ggttgaaaga
     3721 tcagtcacca ggttattaca aattagaaga tttattaatg gacaaagggg tgtaaaaaac
     3781 gtggaagctc aagaaatcat ccgctacatt caaacggcat ctaaaaagac gcctgtgaaa
     3841 gtctatctga atgtaaccga gccaatcgaa tttaaagaaa gtaaagtttt tggtgaagga
     3901 agcagcttcg ttgtttttgg tgactatgag acgattgctc cggttttaga agctgaatca
     3961 gaaaaaatca ttgaaatcga aatcgaaact gctgcaagat acagcgcagt tcctttgcta
     4021 gatattaaaa aaattaatgc tcgtatcgaa ccaggtgcta ttatcagaga tcaagtgtca
     4081 atcggcaaca atgccgtgat catgatggga gcgattatca atattggtgc agtaatcggt
     4141 gaaaacacga tgattgatat gggtgctgtc ttaggtggac gagcgacagt gggcaaaaac
     4201 tgccatattg gtgcaggagc tgtcttagca ggtgtgatcg aaccagcatc ggccaaaccg
     4261 gttatcgtag aagacggcgt actggtaggt gcgaatgcag tgattgttga aggtgtccat
     4321 atcggaaaag atgcagtcgt ggcagcagga gcagtggttt tagaagatgt ggcagcagaa
     4381 actgttgtcg gtggaatacc tgcacgtgta ttaaaaataa gtgatgatca gacaaaagaa
     4441 aatacagcat tgattgctgc gttacgcgaa ttatagagga gatggagaaa aatgcgcgaa
     4501 atagatacct gtattgtggg ggcaaccgga ttagtcggcc aaacgatgct caaagtgtta
     4561 gaagaatatg attttccagt aggacgattg aaattattag cttcaaaacg ttcagccggt
     4621 aaagtttgtg tattcaagaa aaagaaacac actgtacagg aattgacaga aacttcattt
     4681 gaaggatatg atttagcgct attttctgca ggtgcttctg tatccaaaga gtttgcgcca
     4741 atcgcacgaa gtcagggact atttgtcata gataattcct cagcatggcg ggaagattcg
     4801 gatattccat taatcgttcc agaggtcaat ctggaagact atacattgaa tcggctgatt
     4861 gcaaatccaa attgttcaac cattcaagcg gtacttccgc ttaaagcttt acaagaagcc
     4921 tttggtatcc agcgagtcaa ttattcgacc tatcaagcag tttctggttc cgggcaaaaa
     4981 ggcattgaag atcttcatcg tacgcaaaat gacgaggaac cagttttgta tccttataat
     5041 attagccaaa cggtgattcc ggaaatcgat cagcctttgg aaaatgggta tacaaaagaa
     5101 gaacagaaaa tgataaatga gacccgtaag attctgcatc agcctgcttt gccagtatca
     5161 gcaacctgcg tacgtgtgcc aatcgaaaat ggacatggag tctctgtggc agttcaactg
     5221 caaaaagagt ttacgacgga agaaattaga gagtgtctca gccagtttcc agggattcaa
     5281 ttagaagatg atctagcagc acataaatat ccgacgagca ttcttgctag aggaacagat
     5341 atggtttatg ttgggaggat cagaaaagac acttctttag agaacggatt gttgttctat
     5401 acgactgccg ataacatacg caaaggagcc gctgcgaatg ccgtacagat tgctgcagct
     5461 ttaatgcaga aaatagcgga ggtggcaaga tgagtaatac gctgaaagta gcgaaattcg
     5521 gcggaacatc gatgtctcat gaagagcaat tcaaaaaagt ccgtacaatt gttctttcag
     5581 atcccaatcg ccaagtgatc gttgtttctg ctttaggcaa acgtacgtca gatgatgaca
     5641 aagtaacaga cttactttat ttgatttatg cttaccttca gcatcatgtt gattggacac
     5701 ccctttggaa aaggatttgt gatcgtttca ttcaagtaag agatgttctt gccctgcagt
     5761 tacctattga agatgaacta gcacgcatac agaaacaact ggaagacgaa aagataactg
     5821 aagattattt agtaagtaga ggtgaatatc tgactgctca actgatggct gagtatcttg
     5881 gttaccagtt tattgacgca aaagacctga ttttctttga ctatgatggg aaaatcgatc
     5941 aggaaaaaac agcacaggaa atcacaaaag catttaaaag atatccgaaa atggttgttc
     6001 cagggtttta cggggcgaac cctgcaggaa aagaaaaatt attgggccgt gggggaagcg
     6061 acattaccgg cgcgatactg gcgaagatgt tgcaagcgga aagttatgag aattggacgg
     6121 atgtttcagg aatcatgatg gctgatccgc gaatcatcga tcatcctaaa atcatcgagg
     6181 aaatctcttt tagagaactg agagagatgg cttatatggg ggctaacgtt cttcatgaag
     6241 cagctgtttt cccagttcaa gaggctgata tccccatcca aataaaaaat accaatgatc
     6301 ctttcgctca agggacaaaa atatcgaatc acgaaataga aaaggaaaat ggactgacag
     6361 gaatagctgg acgcaaggat tttctttcca tcactttatt caaacgtcat atgtccgatg
     6421 aaatcgggtt tatctggaaa gcaatgagca tttttgctaa acatgagata tcaatcgaac
     6481 atattccttc agggatcgac aatatcggag tgatcgtctc agcagaagcg attgccgata
     6541 agttatttct catcacgaaa gaattaaaag aaacacttgg cgtggaagaa atcgtcgtga
     6601 tcgaagactt agctttgatc tctgtcgttg ggggtccgca taaagaattg attggattat
     6661 ctgggaaagt gttatcgata ttgaacaaat tagaaattcg cacatcgatc ttatcacaag
     6721 gagcacagga gttgaatctg atcatcggtg taccgaataa tcaatatgaa acagtagtga
     6781 agggtatcta tgaagggatg gtgaatacgg atgctgaaag aatatcgaat ggcactccat
     6841 caaattcctg aaatcgggtt tcatgaattc aagacaaaaa aatatttata tgaacaagta
     6901 aaaaacagcg gggccgttat ccatgaaatc ggcgaaacag gactactcct ttattttgat
     6961 aatcagcaag aagaaacgat tgcttttcga acagatatcg atgcactgcc agtcaaagaa
     7021 gaaacgaatc tctcttttgc ttctaagcat gaaggttata tgcatgcatg tggtcatgac
     7081 ggacatatgg cgatgctttt agggttgact gactttataa ctaaacacat tgaggatctt
     7141 atttataatg tagtactgat ctatcagcca tcagaagaaa tttccggtgg cgcacaaagc
     7201 gttattcagt caggattgtt ggataaatat gaagtacaag caatatttgg ctttcatcta
     7261 tggcctggac tagctaaggg agaagttttc tctcgaccag gacctttgat ggcacaaagc
     7321 agtgaaacag acattatcat taaaggccag tctgcacata ttgcttctag tgaaaaagga
     7381 atcgatagtc tggaggcagc tgtacgattt ttgaagaatg tttatgattt tgaccggcaa
     7441 ttaccggcag agtggaatca tctaatgaaa tttggccaaa tccaaggtgg aacgatccga
     7501 aatgttttag ctggagaagt tattgtatcc ggaagtatcc gttcttacag ttcagatatg
     7561 caaaattatt tgaagagggg attagaccat ttagcagaac agtttcactc tacagctact
     7621 atttcttttc gctataatga cggctatcca gctgtaatca accatagagg cttatatgca
     7681 gcactgttag attcaaacaa actacatgaa ttagatgctc cagttcttca agccgaagat
     7741 tttggcatat atacgcaaca atatccatgt gtatttttct ttttaggtgt cggtgatacc
     7801 cctgctttac atgatccgac gttcgatttt gatatggatg ttttagaaaa tggtgtagaa
     7861 tggtacaaaa cgattctttt tacagaaaat cttcttgtaa aaatcgatag cagttcaaaa
     7921 gaggagcgag taaataatga tgaatttatc caatcgattt agtcctaggc tttctagaat
     7981 agaagtatca aaaatacgac aattcgatca gcaaatatcg tcgataccag atgtgatcaa
     8041 actcaccctg ggagaaccgg attttccaac tcctgaacat gtcaagcaag caggaatagc
     8101 agcgattgaa gaagactttt ctcactatac cggaatgaga ggattagagg aactgcggga
     8161 agcggcttgt atatttcagc aacagcgtta tggattaact tatgatccgc aaacggaagt
     8221 tttgacgaca gttggtgcaa cggaagcgat tgcctctgcg ttgttatctg ttttggaaga
     8281 aggagacaag gttcttattc cagcgcctgc ttattcagga tatcagccat tagtggaact
     8341 agctggtgct gaattgatcc cgatagatac ttctgacaca gggtttgtct gtcagccgga
     8401 acaatttgaa cgagcatttg aacaatatgg atcagctgtc aaagcagtca ttttaaacta
     8461 tcctaacaat cctactggga cgacattgtc tgcaaaacaa ttgaaagcaa tcgctgaggt
     8521 tttaaaaaaa tatccggtat ttgttataag tgatgaagta tatgctgaat tgacttatag
     8581 tggaacgcat atgtccatag ctacttatct tccagaacaa acaatcgtga taagcgggtt
     8641 atccaagtca catgcgatga ctggttggcg agtgggcttt atttttgctc aaaaagccct
     8701 tattgacgaa ctaattaaag tccatcaata tctggtgact gccgctacaa cgatgtcgca
     8761 aaaagctgcg gctgaagcgc taataaatgg ggtgaaagat agcgagaata tgaaagagca
     8821 atatcaaaca cgaagagact atctggtcca gcaattggcg cctttaggat ttgaagtgac
     8881 ccagccaaac ggtgcatttt acttgttttg caaactccca gctgccattc aaactgactc
     8941 ttggcagttt tgtgtagaat tagcggaaca ggcaaaagtt gcgtgtatcc caggaattgc
     9001 atttggccca gaaggagaag gttatatccg gatttcttat gctagcagca tggaaaaatt
     9061 acatgaggcc tgcaaacgga tcaaagactt tttaacagaa cagtgattca tagattttat
     9121 caaaaaaaca atcgttaatt ttgcggtata ttctgcaagg agacgattgt tttttaaatt
     9181 tagttcaaat gaacgcgttt cttttgattc ataactaatg taatgttaag tttaaattgt
     9241 ttagaatgat tttaaaagaa tattcaagga ggtttcatca atggaaaaaa tcaaagttgt
     9301 cattgtaggt gcatctcatg gagggcatca atcgatcttg gaactattga cacgatacgg
     9361 tgaaaatgta gatatcacgc tatttgaagc aggagactat atttccttca tgtcatgtgg
     9421 tatggagctg tatttagaag acaaggtaac agatgtaaat gatgtacgta atttcagacc
     9481 agaaaacttt ccgcaatcca atgttcatat tttaaataat cacgaagtaa aaacgatcga
     9541 tgccgataaa aaaacagtaa cggttactag cgcatcagat aacgaaacaa aagaatatcc
     9601 ttatgacaaa ttgattttga gttctggtgt caaaccaaac aatctgccag taccaggagc
     9661 agatctagaa aatgtctact tgatgcgtgg atacaattgg gcaaccaaaa tcaaagaaaa
     9721 attaaatgat ccggctatta aaaaaatcgc tgtcgtaggt gcgggttaca ttgggatcga
     9781 agcagcagaa gcaagccgaa aagctgggaa agaagtcgta ttgcttgatg tgatcgatag
     9841 acctttaggc acttatttag atgctgagat gacagatatt ttagaacaac acctaaatga
     9901 aaacggtatt gaagttttga caaatgcaaa aatcgaagct tttactggta acggaaaagt
     9961 tgaggcaatc aaaacaagtg aaaaagaaat cccagcagat ctagtcattc aagctgccgg
    10021 tgtgaaggca aatacagaat ggttaaaagg gatcgtcgac ttagatgaac gtggctggat
    10081 ccaaacaaat gaatacttgc aaacgaatct tccagatgtg tatgcagtag gagatgctac
    10141 acttgcttat tctatcccag cacgcaaaaa aatgccgatc gctttagcga ctgttgcacg
    10201 tagagaagca cgttatgtag taaaacatct ttttgaagaa gtaccaaaca aaccgttcgg
    10261 cggagtcgta ggttcctctg cattaagtgt ctttgactat cattttgcag caagcggatt
    10321 gaatagcttt acagcagcaa aagcaggagt agatgtacga gtgtcttatt atgaagatac
    10381 gttacgacca aaatatgtac cagaagaaca aggaaatcca aaagtgtcca tccaattatt
    10441 ctcggatcca atgacccatc aattgctagg aggagcagta ttatctacct atgatgttac
    10501 aggtcaagga aatgtattgg cactagcgat ccaaaacaaa ctgactttag aagaactagc
    10561 agaagctgac ttcttcttcc agccaggctt tgaccgacaa tggagcatgt tgaatctagc
    10621 cgctcaacaa gcgttaggag aagaaccatt tgtagaataa gaaaacgaaa tatttgaaaa
    10681 gaggttgtga aaaagctgcc ttgcatcaag taataagaac agccaagtaa aaacagcttc
    10741 ttatgttttt aacttggctg gacttattac cgcagatgca agcttttgaa caccgtttat
    10801 tttcaaaaaa gggactgtga cacgatttgt gtaccgaccc ccaaaagtta gaccaaaaaa
    10861 tctaactttt ggaggtcggt attttttatg gttaaatata cttttgaact taaattaaag
    10921 atcgttcatg attatttgga tggcaaaggc gattggaccg taaaaatcca gatcaagaga
    10981 ttgaagcgga aatgcttaaa attcgggaga agcacaagga ttatggctgt ttacggatga
    11041 ccaatgaact aagaaaccgc ggtttctcta tcaataaaaa gaaagttcag cgcttgatca
    11101 agaagctagg aataaaagta acggcataca cgcgtaaatc acgtcgctac aattcttatc
    11161 gaggaaaagt
//
LOCUS       DACDEG010000080        11120 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.084, whole genome shotgun sequence.
ACCESSION   DACDEG010000080 DACDEG010000000
VERSION     DACDEG010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.084"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            390..1829
                     /locus_tag="IV142_002135"
     CDS             390..1829
                     /locus_tag="IV142_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8485764.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            2124..2342
                     /locus_tag="IV142_002136"
                     /pseudo
     CDS             2124..2342
                     /locus_tag="IV142_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2591..2806
                     /locus_tag="IV142_002137"
     CDS             2591..2806
                     /locus_tag="IV142_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485765.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(3023..3279)
                     /locus_tag="IV142_002138"
                     /pseudo
     CDS             complement(3023..3279)
                     /locus_tag="IV142_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(3466..4377)
                     /locus_tag="IV142_002139"
     CDS             complement(3466..4377)
                     /locus_tag="IV142_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8485766.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(4396..5400)
                     /locus_tag="IV142_002140"
     CDS             complement(4396..5400)
                     /locus_tag="IV142_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP8485767.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(5397..7520)
                     /locus_tag="IV142_002141"
     CDS             complement(5397..7520)
                     /locus_tag="IV142_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8485768.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(7525..9972)
                     /locus_tag="IV142_002142"
     CDS             complement(7525..9972)
                     /locus_tag="IV142_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8485769.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(9959..10348)
                     /locus_tag="IV142_002143"
     CDS             complement(9959..10348)
                     /locus_tag="IV142_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8485770.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(10408..10911)
                     /locus_tag="IV142_002144"
     CDS             complement(10408..10911)
                     /locus_tag="IV142_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP8485771.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(10924..11085)
                     /locus_tag="IV142_002145"
     CDS             complement(10924..11085)
                     /locus_tag="IV142_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002392227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485772.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
BASE COUNT         3245 a         2334 c         2002 g         3539 t
ORIGIN      
        1 tgatatttag tataatccta tggaattgaa tatcatagca atgtatttga aaagcaaatt
       61 ggaaaacaaa taggtgcaac accataaata tgaaaaaatt gggaaaaagt agaatggaaa
      121 tttgcttacg tctcacgaga tagctcacac tattaatctg aagaacaatg aagtcgactt
      181 ttagaaattg ctagaagaaa gtaaagcgaa ttatcactag ttactataga gtaagttcaa
      241 ataaagcatc tccaacttac atggttacat gaggatatca aatcgaaatt actcaagctt
      301 cctatggcta gagtcgcgag aagttgttgg gcagtagaca taggttatat tgttcttagc
      361 tacacgatca ttagaaagga aatcctatta tgtatagtta tcaagaagat gcaaaagtcc
      421 aaaaaaatcg ctggtggatt ttgattgcta tatctatgtt taccttcatg tccacattgg
      481 atggcagtat tgtcaatatt gcactaccta ccatctcaga tgatatgggc ataccaatga
      541 atcaagcgga atgggtagtg tccgtttatc tgatgattgt ctgttcttgt ttgttgctgt
      601 ttggtaaaat tggcgatagc cgtgggaaaa tcaaagtatt tcggattggt acgctgatct
      661 ttgtcttggg atcgctgttg tgtgggttca atcattcgct aggtatttta ttgttcgcac
      721 gggtggtaca agccattggt gctagtatga ctatggctac aaatgccgga atcatcacgg
      781 aagtctttcc catacatgaa cgtggtcggg cattgggagc aataggtgcg tttgtttcat
      841 taggctcgat tgctggacca ggtcttggcg gcttgatttt gtcccaattt tcttgggcgt
      901 acattttttg gatcaatgta ccggtgggaa tcgtaacgat gttgattgct gaaaaattct
      961 taccaaaaga tatcaccatg agcggccata caatcgatgg agggggcttt gctgccattg
     1021 cggctacgat tatcagcttt tttggagcaa tctttttggg gcaagaaagt ggctttttcc
     1081 atccagtccc ccttgtactg ttcgtaattt cagtgattgc ctttgtcagc tttttaaaag
     1141 ttgaaaagaa agcagcctct ccattgattt ctttcggtat ttttaagaac aagattttta
     1201 ccatgagctt gattacagcc ttgttgattt tttcttccaa cttctttgtc aatgtagtga
     1261 ttccttttta tctgcaaaat gctcgcgggt tgagtcctag ctatgctggg ctgttgatga
     1321 tggtctttcc tttgatgatg gtcgtcgggg cgcctttaag cggctacctg accgaccggg
     1381 ttggcccgga gattttggta ttagtaggct tgggcttatt gtcggtaacc caggtcatgt
     1441 atatgttttt gtcagtggac tctcctttgt ggctatacat tgtcgctact gccatcatgg
     1501 gtgccggcaa tgccttgttc cagtccccca ataatacgat ggttatgagc tcggtcacta
     1561 tcgaaaactt gggactggca ggctcgatga attcctttgc ccgaaatttg gggatggtca
     1621 gcgggatttc gctttctacg acggtcttat atcaagcgat gtctgctaag atgggccagc
     1681 aagtaactaa ctatatcgca aatcggccgg atgtcttttt atatggaatg aagattacct
     1741 ttttaggcag ctttgttttg tgttttgttg cctttttcct gactatctgg cgtatcaaaa
     1801 aacaaggagg tattcagaaa aatgtctgaa aaaaccactg ttgaagaatt tttggaggcc
     1861 tgcgtatggg tgagtgggat ttccatggat gatggaagat gaagtgaact tctaaaagcc
     1921 gaaaaactca agtattatct tcaaaatact agtgctcaat gagatgcttg atttgatcac
     1981 aagaagactg cgaaagccgc tggcaaccgc aagctaggta tcccaccgat ggactacgtt
     2041 ttagaggcta ctggttatga atacggagcc aacgctctca tctgcataag aatacatcac
     2101 ccagattatg taaagatttt cgatacttct gtcaaaaaat cattaagata gataaatgat
     2161 ttccgtctag tcaaattggt tcagaatgta gacacacaga tggtaagaag tcactcacaa
     2221 ttcgagaatg gacttgtcct atttgccgta tacatcataa tcgaaacgtc aacgatagta
     2281 tcaatatttt gaccaagggc tatgaataca agcattgcct tagaaaacat caagaacctt
     2341 aggaacaacg gggatagctt ggttaataag agataccgct gtttaggctt gcaagtatac
     2401 tcttttatca agaattccgt ggctttagtc atgagaggct taatactatg aaaattttct
     2461 ttagcatttt tgatttaggt tccttttcta attataataa gatgaccact acagtaacct
     2521 actgaaatat ataaacagcc actgtagtac cattttcaaa gtagtctatg agaggagaat
     2581 cttcattctt atgaacgctg gaattgcgga tatgaattta ataaaaaaaa cccttaatga
     2641 ttttacttcc aattcaatat caaaaggtac tggtatcaat ttaagcacaa taaaaaagct
     2701 gaaaagtggt gaaagatcag tggaaaagct gaatctttta gatgctataa aaataacgga
     2761 atttgctatg aaaaatggaa aagctgagat tgaaatatgg agataattat cgtctagtga
     2821 attacttgcc acttagctga atttaacgat ccttggtgct tgaaggggga gattcttggg
     2881 aatagagcat acttgcaagc ctatgtcttt actaacaacg atatctctta ttaaccaagc
     2941 tatccccgta gttctaaggc ttttactttt atctaagcaa taatcgtatt caaaggaaat
     3001 ttttgaccct tagaaagtct cattttatca gacgtgagct taggcattgc tgaattttgt
     3061 taattcggtt ttaggttgtg ctttgagcat aatatgaaag tattctttat cacgatttca
     3121 ttcatctaag gtaatgtgat gtgattctga aattctttca aaggcagttt ttgcataagc
     3181 tgacattaca tcatcaatga catgtttaca atgtttcact actaaaataa gggggtaacc
     3241 aagaagaaaa actgaatgat tatttgtatc taattccatg gtatatcaac catttcctat
     3301 atagactgat tatgacatgg attaaaataa aaaggcacta tgtcttaatt gtcggttttc
     3361 gagttaccga atggcattca tcacccacct atagaggatg ggcgacttct gcctgaaata
     3421 agttacacta cttataactt ctaataaaat tagattgagt cattattatt taacatcaac
     3481 gattttccag tttccatttt tttgtaatac taatttgtac tgaaagtagc tagtggtttt
     3541 tgatacatta tcaaggtatt ttatctttgt actcactaga aaattttttc cgttttgtag
     3601 ataaactgga ttctctaact ccgaaaactt taagtcaata ttgattggtc gcatcgcacc
     3661 attttcaaca taatactcca attctttttc agaagctgat ggataaagtt taaaaaatgt
     3721 ttctaaaaaa tcggtgattt cttccgttgt tttggcatca atttctgaat cgttttctac
     3781 ttgttttgct tcatattttg ccttgtctgg ctttccaatc attgttggca aactagttac
     3841 taaatgattc ctgttttcat cttcaaagat tgtcactcga tatgcagaag aaattgattg
     3901 cgtctttttt ccctctgtaa tatgttgctc cactaaaaat gaaacgacaa attcttccga
     3961 gtgtttttca acatctagaa tttttacaga ttgaacttcg gaactcgtgg gaatatctgc
     4021 tcgaaccata tcttggatta aagcgagacc gttttctgta aggtactgtt cgagattact
     4081 cattctcttt tcaattgctt ctttgttatt tttccatgaa aaatattctt tcgcaaaatc
     4141 ctctgtaaaa ctttcaacgc cagaagtatt cactaccttc tcttttataa ccactttttc
     4201 atgagttgta tgttgatcaa tggcggtgaa atttttataa acgccaaaag caagactaca
     4261 agacaataaa agccaaaggg caagcaccat ttttcgatgc ttacccacgg atacaactcg
     4321 ttcttttttc ttttttgggc tcttttcctt ttgattccgt tcaattttta tcttcattct
     4381 ttttcctctt ttctcttatt tgtatcggcc agctccgata agatgctgcc gccaatagtt
     4441 ttccgttaga ttcgtccacc ccactggatc tcctgcatga aacatgcgat tctcccctgc
     4501 gtaaatgccc acatgggtca cataagtacc ggcatcatag gttgaatgaa agaaaactaa
     4561 atcacctggt ttggcatctt ttagatcaat atgagtcatg gcatcatatt gatcctgagc
     4621 cacccgagga agtttcaggt caactttggc ataacaccat tgggtcaagc cactacaatc
     4681 aaaccccgtt tctggacttg agccaccaaa cacataggcc gttccttcat acttaaatgc
     4741 ctcatcaaaa atttcttgga ctattttgtc atcaaactga gttgtagtta ggtattgctt
     4801 cactaataaa cagtaaaaca tattgccata attataccgc cacccaccat tttccttaat
     4861 cgcgattggg ttggggtaat cgactttttg accacctgat ttttcctttg aaaaagattc
     4921 agctaaagca aaactatgct tcttcccgtt tttcccgaca tgatctagat acccgccacc
     4981 atagttatag gcctgaataa tactatcttg atcacaccct agttctttgg aacgcttaac
     5041 caaagacgca aaatagagac agccttgttt gatggatgcc tcggtactta atgaattagg
     5101 tggtaatccg gctgactcag aactttgcat aacgtcttcc attgtgccac cagattccac
     5161 ttgaataatc gccaaaagaa ttggaatata ctctgttata tccgattctt tggcatactt
     5221 ttccaccatt gcacggtgtt tcaatacttc ttctgagaca ctagttccgc caggagaagt
     5281 gccagaacta ccatttccct cactgtcttc accaaacaac aaacccacac acatcaaaat
     5341 acccataaaa gagatggtca gaaaagttaa tgaaataaca cccactttct tcaagttcat
     5401 tttttctcac ctgaccttga ttttggacga tggttcattc ttttttgtac tttttgattg
     5461 gtctgctttg gcagagtgga cctctgtttc acttgttgga aagaattttt cttacttgct
     5521 gattggcttt tcccttttga aatgatttct tgactagtta gttttcgttt gatttcggga
     5581 ttcagatttg ctctcacaat ccgctttggt gcttcttttt tcagcatcat atctttctga
     5641 ggttgagctg ctttatctat tggaatattc gcccttcttt ttccctgacg atttgttgct
     5701 tcactcagta tcttgcgttt ttcgtccatt ttcgcatttc ttcgttgtcg ttgtttttct
     5761 cgcaaatcca ctcgatccgc tttttcttgg aacagtccgc gcttgaattc ttctggtgct
     5821 tttttcgact tttcgattcc tttatggaga ttgtatttca aattcgttgg cgtattcctt
     5881 acctgatcct ttacttgttt agtcttatct ttcaccaaag cttgggtatc caaaactttg
     5941 ccgatttttc tcccagccaa attcattcgg gattgtttct tattatttga aactggattc
     6001 tccttagtta agtcagatag tatttcgtga tcgttcggta atcgtgaatt ctttcgtaga
     6061 gaagaaccgg atggcttaaa tttggatttt gattgttcct ttgccaactt atttccgact
     6121 gctgcaccac caccaaaagc caaagctcgt cccacatttc gattgagttg ccgcaatttt
     6181 cgattcatta acatttgcgg ttttcgcatg acgcgtcttc ccattgactg gctatcatta
     6241 ctctggaggt tgaacatact cataatgtcg cccaatttga aatagatacc ggcaaaggtc
     6301 acaatttgca gaaaagcaac catgaaaaat ggataggttg ccgaaatatt gaaaaacatc
     6361 gtggagatgc taaatgcagt cgtaatcacc aaagtaacac ccgctcgcat cataatcgta
     6421 ttgaataaac gaataatcgc cttctttcct aaactttcat aagtcggcaa catggacaat
     6481 aagaaactaa ttggcagaaa catcgcaaag ataatgaaga gaatttgcga gaacaacata
     6541 atccctgtta gaagaaaaac aaagaaggaa atgccaatat tgaacaatac caaaaagacc
     6601 acggtcccta aacggttcat ggtcttggta atactgagat tcgcattgtc attgtcttca
     6661 atctctgcct tgaccgcttc ttcccgatct ttgcctttgt tttcatctgg actcacagat
     6721 agaatcttgt tgacccggtc ttcaccaatt tcttctatgt tggaatcatc aaattgcagt
     6781 agtaaccaag gttgttggac ttgaatcgaa aacaaactat cccgaatcaa atccacacta
     6841 tcttttcctt ggctttctga attgggtaca acgatctcgg ttccaagggt aagggccgct
     6901 tcactaacat ctgagctaaa gtcattgatt tttgtgatat aggtaggcgc ataagcaatg
     6961 aacgaacccg atagtagaaa aatcatcaca aaattaatca cggctcgaac tgctttcgtg
     7021 gtttctcgtt ttaataatcc ggtatacgcc acatacaccc ccagagctaa aatcatcaat
     7081 aatagaaacc caaaataaaa tccggaggtc tggagtccat tttcagtaat accagccaat
     7141 gtttggatat tctttccaat actttcagcc gtatccgaaa tgaaatctaa tttgtaagcc
     7201 tcttgaacca cataacccgt ggcgttcgac aaatacaaac tcaccgtcca aatgaaatta
     7261 gtaatggcat aaagaccata catcacgctt ttccctaagc catcacccca attccaaggc
     7321 aaccaatccc aagagctatc cacaaagaaa tctagttgat agttgttcag tgaatatttg
     7381 gaatatagat ttcctgcttc aactgtatca tcaactagcc ctgtcgcctc ggctaccgta
     7441 ccaactaaac tcaatagaac tatcagagtg agtatgctaa taaaaacgat tcccacaatc
     7501 cgaaaaagtt tctttttcat cccctcactc cattctctct tgaacaggtg gacgggtatc
     7561 aaaggcatgg aagagttctt caaagactgg atgtacctga accacgccta cccgaccata
     7621 caaatcctgc atcaagcatt ggccattttc caaatctcgt aggcgttttt gattgctttc
     7681 atcttctcga tccaagccaa agaattccaa ggttttcttg atttcatgaa tatcggtact
     7741 gcgaaacgca aacttcaacc cgatattatt cttcaagcgt tcatccagta aatcatcact
     7801 attttgcgtg acaaagtaaa ccccggcatt catagcccgc ccagcacgaa cgagcttatt
     7861 cgataatgtt ttcccttgcg ctacttgaag aaaactccac gcctcatcta agtctacaat
     7921 tttgaagatt tctcgattac tatggataaa gtccaaggca aaagtggaaa tgacgattag
     7981 catggaaaca ctaagcaatt ccatggtggt atattcttca aaagtggttt ctgcatctgg
     8041 taaaaccaaa tccgctactt gaatgatatt taactgccta tctaaactaa tcgactgggt
     8101 aatcgaacca tctgagaaaa gcaaatgggc aaaatcataa tcggtaaagg actcgatgtg
     8161 atccgcgata ttttccgcaa ctggtgagcc ttcttttcga agctcattaa ttacttgtaa
     8221 taatccccgt tgattgcttt gggtaaccgt ccgaatggcc ttacgcaaca ctggaaactt
     8281 ctccccatct cgactagaaa ttcctgttaa aaaggtcaga atatcaattg ctagactttc
     8341 agaatccttc tttttctcca taatcacata aggatcaagt aatccttgat tttttggctt
     8401 actagttaag ttgagaatat tgatttcatc cgcaatttct ggcaaggttt ctttccagcc
     8461 accccgttcc ccttttggat cgacaatcac agcttgtcca ccaaaaagga cggcgtaata
     8521 aactaagaga ttgttactaa aggactttcc gccaccgagt gaaccgagga atgccgctgc
     8581 tagcgcattg gtaactgaac ccttgacccc ttgtgaggcc aatgccggtt tcaggtaaac
     8641 attgcgcccc gtatctacgt tataaccaaa gtaaatgcct tccaactccc ctaacatctg
     8701 agtggcgcca aaaccaagtc cggcgagaaa atcagacgta acatactgga tataatcatt
     8761 catgtatcgc ttactagaag gtaagaattc ttcatgtagc cccatcatat cgccaaaagg
     8821 ccggactagt tttacattgg tatcatcata aaaatctagc acttcatcac accggcgctt
     8881 tagttcatct actgattcgg cacttactcg aaccacatag ctcagtttgt acatggattc
     8941 tttggactga tccaaggtag tttccaattc atctacggaa tccaatgcat ctaagacatt
     9001 ggaatttgtt tcattgtcag attgataggc atggttgtcc aagtctttta attccttttt
     9061 cttattgcga acggtagcaa gtgccttttt gttcgtcaca atctccacat tcatactagt
     9121 gtcaattggg aatgtaaatt gttgttgctg gtaatagaaa agttctgagg agggaaactc
     9181 catttccccc acaattgtat taattgttaa gtacgctaca tatgattcat gattctcatg
     9241 ttccatgcat aagtaccggg gcttttcttc aatcaaacaa cgactgggac gtaataagtc
     9301 gtaacgtttc accaaagttt ccgacttcag ctttttcttt ggtagctgaa attcatattc
     9361 ttcaaatggt gtaccctttt cgccatagat atgttcaatc aaatacatca gatcactcgg
     9421 tgtgactcgt ctgactttaa atcttcgagc caatttactt tccattagtt tttcaagctt
     9481 ggtatatcgg cgaatttctt cattggataa ggaaacaaaa tcgcccatca aatgatgatt
     9541 cactccatag acaaattctt gaaagccgga gaagaaagat ttcttgagat tctttagatt
     9601 gacctcttca tctgtcgcaa tcagtttgaa gccaataaaa aagcgataat caatctgact
     9661 atcgccaatc atggatacta atgcctctgt ctgtaaatca attcgttgtt tcgctacttc
     9721 tgttaatcgc ccggtaattt ccttttttga tcgttcttgc gtggcacgaa tactactttc
     9781 tgtggcaatt tgtaaggcat gaatcttgcc ttcacgattt tgcgcaatca attgacgaaa
     9841 gttatcgtgg acttgatatt tctgttcagg tgacaaaaag gagtaattgt aaggtattaa
     9901 ctcatagtag gcaaaacatt ctccctcttg attaaagaca agattatttt caatatactt
     9961 aatcgggtac atagttcaca ctccttacca gagtaatggg ttcgtcccat cgtttcttct
    10021 gttctttgac tttctttcct gcgaaagtga ccttaaatcg taagtgatag ctaataaaag
    10081 atcgtaaaaa tcccaatggt ttcttaccgt caaacgtttt ttgcgacatg aaccaagtaa
    10141 ctgccaccgg tatacctaag tattttaaaa aggccccctc tatcatagat agtggaggca
    10201 catgggcaaa tacgatgaca aacagcaagg ataaaacaaa ccaagccatc tggttaaaag
    10261 tcacaggaaa aggcagttga aagtcattaa ttgcataaat gaccttttca actgcccaaa
    10321 tactggtgta gctttttatt ttcttcatca aatcattcct ttaaaaagaa ggatagttta
    10381 gaaatcaact acccttcaat aattttgtta atatggatac tcaaaaacac ctcgactcgt
    10441 taccaaataa ttgcctccaa tctccaagtc tctaccacac gcatcaaaat caatatacgt
    10501 cttcaagtgt tctggtactt ctccaaaatc accagcttct acaatcaaga aatatgctac
    10561 atcactcata tcatcgcaat ctggataata gataatatca tccacatgct ctaccatctc
    10621 ctcaaaagaa ttgaagaaag catgttgaat ctcagaagca acttcaccaa ctggcgttcc
    10681 ttcaagttcc tccgctaagc cacacaaccg attaatttcc tcaattggcg tatactcatc
    10741 aatctcaaag ggaagttcat aatcatgaat tgcatattcc tcgtattcgt catttaaccc
    10801 aattcgttct ttcacttcat caaaatcgac aggtggggta aaccacgccc ccaccaattc
    10861 accttcgtta tatttcccga ggttcgcaat ataaacacgc atctgttcca tcgtgcttcc
    10921 cccttacgca ccgacaattc ggttaaacaa actgagtaag acatccttca ccccagaagc
    10981 attaaagacc aatccgaccg caatcaaagc aatcaccaaa aagccaatca gtttggaaaa
    11041 ctcccgctta aatcctaaaa acaaaccaat gaccacaatt gccagtaaga ctaaggattg
    11101 ggcattggtt aaaaaccatt
//
LOCUS       DACDEG010000081        10927 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.085, whole genome shotgun sequence.
ACCESSION   DACDEG010000081 DACDEG010000000
VERSION     DACDEG010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.085"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            8..81
                     /locus_tag="IV142_002146"
     tRNA            8..81
                     /locus_tag="IV142_002146"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="IV142_002147"
     tRNA            101..173
                     /locus_tag="IV142_002147"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="IV142_002148"
     tRNA            201..274
                     /locus_tag="IV142_002148"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="IV142_002149"
     tRNA            288..361
                     /locus_tag="IV142_002149"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="IV142_002150"
     tRNA            385..474
                     /locus_tag="IV142_002150"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="IV142_002151"
     tRNA            487..560
                     /locus_tag="IV142_002151"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..654
                     /locus_tag="IV142_002152"
     tRNA            579..654
                     /locus_tag="IV142_002152"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            656..726
                     /locus_tag="IV142_002153"
     tRNA            656..726
                     /locus_tag="IV142_002153"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gly,seq:tcc)
     gene            740..813
                     /locus_tag="IV142_002154"
     tRNA            740..813
                     /locus_tag="IV142_002154"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Ile,seq:gat)
     gene            838..925
                     /locus_tag="IV142_002155"
     tRNA            838..925
                     /locus_tag="IV142_002155"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ser,seq:gct)
     gene            947..1018
                     /locus_tag="IV142_002156"
     tRNA            947..1018
                     /locus_tag="IV142_002156"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Glu,seq:ttc)
     gene            1041..1130
                     /locus_tag="IV142_002157"
     tRNA            1041..1130
                     /locus_tag="IV142_002157"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Ser,seq:tga)
     gene            1143..1216
                     /locus_tag="IV142_002158"
     tRNA            1143..1216
                     /locus_tag="IV142_002158"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Met,seq:cat)
     gene            1235..1307
                     /locus_tag="IV142_002159"
     tRNA            1235..1307
                     /locus_tag="IV142_002159"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Asp,seq:gtc)
     gene            1311..1383
                     /locus_tag="IV142_002160"
     tRNA            1311..1383
                     /locus_tag="IV142_002160"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Phe,seq:gaa)
     gene            1388..1458
                     /locus_tag="IV142_002161"
     tRNA            1388..1458
                     /locus_tag="IV142_002161"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Gly,seq:tcc)
     gene            1471..1544
                     /locus_tag="IV142_002162"
     tRNA            1471..1544
                     /locus_tag="IV142_002162"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1505..1507,aa:Ile,seq:gat)
     gene            1556..1627
                     /locus_tag="IV142_002163"
     tRNA            1556..1627
                     /locus_tag="IV142_002163"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Glu,seq:ttc)
     gene            1893..3086
                     /locus_tag="IV142_002164"
     CDS             1893..3086
                     /locus_tag="IV142_002164"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP8485773.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3216..4688
                     /locus_tag="IV142_002165"
     CDS             3216..4688
                     /locus_tag="IV142_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8485774.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4771..5559
                     /locus_tag="IV142_002166"
     CDS             4771..5559
                     /locus_tag="IV142_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP8485775.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5626..6294)
                     /locus_tag="IV142_002167"
     CDS             complement(5626..6294)
                     /locus_tag="IV142_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP8485776.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6409..8121
                     /locus_tag="IV142_002168"
     CDS             6409..8121
                     /locus_tag="IV142_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8485777.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8124..9896
                     /locus_tag="IV142_002169"
     CDS             8124..9896
                     /locus_tag="IV142_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8485778.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            10022..10471
                     /locus_tag="IV142_002170"
                     /pseudo
     CDS             10022..10471
                     /locus_tag="IV142_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(10601..>10927)
                     /locus_tag="IV142_002171"
     CDS             complement(10601..>10927)
                     /locus_tag="IV142_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8485779.1"
                     /translation="GKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVE
                     FVHQYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSD
                     TSGEAA"
BASE COUNT         3225 a         1951 c         2466 g         3285 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gccatgcggg
      661 tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg attctcgtca
      721 cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg cctgataagc
      781 gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat tggttttggg
      841 gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg aaaccggcgc
      901 gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc cgttggtcaa
      961 gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt acgggtcatt
     1021 aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa
     1081 aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct ttttcatatt
     1141 atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt cgtaggttca
     1201 aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg ggttaacatg
     1261 cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt ggctcggtag
     1321 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt ctgtcccgcg
     1381 ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt gtcgcgagtt
     1441 cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt tagagcgcac
     1501 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt ttattggccc
     1561 gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta
     1621 cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga atgattttaa
     1681 aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga cagttctaat
     1741 gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
     1801 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
     1861 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
     1921 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
     1981 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
     2041 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
     2101 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
     2161 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
     2221 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
     2281 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
     2341 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
     2401 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
     2461 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
     2521 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
     2581 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
     2641 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     2701 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     2761 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     2821 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     2881 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     2941 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     3001 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     3061 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     3121 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     3181 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     3241 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     3301 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     3361 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     3421 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     3481 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     3541 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     3601 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     3661 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     3721 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     3781 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     3841 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     3901 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     3961 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     4021 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     4081 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     4141 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     4201 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     4261 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     4321 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     4381 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     4441 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     4501 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     4561 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     4621 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     4681 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     4741 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     4801 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     4861 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     4921 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     4981 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     5041 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     5101 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     5161 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     5221 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     5281 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     5341 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     5401 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     5461 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     5521 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     5581 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     5641 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     5701 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     5761 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     5821 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     5881 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     5941 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     6001 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     6061 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     6121 agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     6181 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     6241 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     6301 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     6361 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     6421 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     6481 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     6541 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     6601 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     6661 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     6721 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     6781 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     6841 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     6901 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     6961 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     7021 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     7081 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     7141 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     7201 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     7261 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     7321 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     7381 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     7441 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     7501 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     7561 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     7621 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     7681 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     7741 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     7801 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     7861 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     7921 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     7981 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     8041 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     8101 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     8161 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     8221 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     8281 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     8341 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     8401 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     8461 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     8521 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     8581 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     8641 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     8701 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     8761 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     8821 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     8881 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     8941 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     9001 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     9061 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     9121 atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     9181 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     9241 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     9301 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     9361 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     9421 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     9481 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     9541 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     9601 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     9661 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     9721 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     9781 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcat gagttattag
     9841 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa agttaaatta
     9901 aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt ttttataagg
     9961 ggcagaaatg atgtgcaccc caaaagttag actagaaatc taattaaaag gggtgctttt
    10021 tttgcggaaa tatacatttg tttttaagaa aaaagtagtt tcagactatt taaataacga
    10081 aggcggctac aaatatcttg cacataaata tcaaataaat cgtacactgg ttagacattg
    10141 ggtaaggatt tataactatc atggttggga aggcttggtt ggaggtggca aaagctacac
    10201 tacaaaattt aaacttgatg ttatagaata tatggaaaca aatggtcttt ctatccaaga
    10261 aactgctaaa aaatttaata tcggttcaaa tagaactcta agtaaatgga tagagcaata
    10321 tgaagaaggc ggtgcttctt cacttgagag ccaaaaaagg ggcagaaaaa ttagtatgaa
    10381 ttccaagcta aacattccta aaaaacttaa agatgagtct cttgaagaag aagttattcg
    10441 tttaagagca gagaacgcat atttaaaaaa gtggaatggc aacacttttt ttgacaaaaa
    10501 ttataaggtg ttgtgaatgc tgcggtgtta tggctacgat tgcccttgac aatgagcatc
    10561 ctcggatctg attttctccg gcagaagcac aatcattcaa ttacgctgcc tctccagagg
    10621 tatcagatcc gcgctcatta tcaaggattc gcttcgccaa tctctgttgt cgaatttgga
    10681 tcggtgtttc atacgccaac gtgccatgta agcgtagata gttccaccaa tgcacataat
    10741 caaacaattc tagacgtagc tgtgccagtg tctcaaattg gtattgatgc acgaattcta
    10801 ctttgacaga tttataggtt gattccacaa cggcattatc atacggacaa cccttcctac
    10861 tcaatgaacg agtaattcca aaaccattca gaatctcatg aatggtttgg ttatcaaatt
    10921 cctttcc
//
LOCUS       DACDEG010000082        10769 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.086, whole genome shotgun sequence.
ACCESSION   DACDEG010000082 DACDEG010000000
VERSION     DACDEG010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.086"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            119..313
                     /locus_tag="IV142_002172"
     CDS             119..313
                     /locus_tag="IV142_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8485780.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            316..1518
                     /locus_tag="IV142_002173"
     CDS             316..1518
                     /locus_tag="IV142_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8485781.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(2101..3732)
                     /locus_tag="IV142_002174"
     CDS             complement(2101..3732)
                     /locus_tag="IV142_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP8485782.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(3746..5419)
                     /locus_tag="IV142_002175"
     CDS             complement(3746..5419)
                     /locus_tag="IV142_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8485783.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(5432..5749)
                     /locus_tag="IV142_002176"
     CDS             complement(5432..5749)
                     /locus_tag="IV142_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8485784.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(5774..7174)
                     /gene="lacG"
                     /locus_tag="IV142_002177"
     CDS             complement(5774..7174)
                     /gene="lacG"
                     /locus_tag="IV142_002177"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP8485785.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            7411..8196
                     /locus_tag="IV142_002178"
     CDS             7411..8196
                     /locus_tag="IV142_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8485786.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8199..8891
                     /locus_tag="IV142_002179"
     CDS             8199..8891
                     /locus_tag="IV142_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8485787.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(9165..9536)
                     /locus_tag="IV142_002180"
     CDS             complement(9165..9536)
                     /locus_tag="IV142_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP8485788.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(9936..10166)
                     /locus_tag="IV142_002181"
     CDS             complement(9936..10166)
                     /locus_tag="IV142_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485789.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(10260..10598)
                     /locus_tag="IV142_002182"
     CDS             complement(10260..10598)
                     /locus_tag="IV142_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485790.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
BASE COUNT         3427 a         2262 c         1822 g         3258 t
ORIGIN      
        1 gagttgggaa gtcctgaaca agtgatggat tttcaagaaa gtaccgttca ttatacaaaa
       61 ataaagaaat aacactatta cccaatgata gtgttttctt tgaaagtgag tttatacaat
      121 gaaaaataca atttgtcgaa aagatttaga ggctctagga tttaaaacac atcaagctac
      181 cacaataatc cgacaagcaa aaacaagaat ggtaaatcag ggatatccat attataataa
      241 taaaagagtt ggggtagtac cccgacaagt aattgaagaa attataggtg ttcctctttc
      301 acaggaggaa taactatgaa aacaaaatac ccttgtgtgt atcaagataa aaatggtcgc
      361 ttttcttatc tagttgagtt aggaacagat caagtgactg gaaagaggat ccagaagaaa
      421 ggaacaaaag attctctagg aaaacgattc tcatcagctc gtgaagcaca caaagaagtc
      481 atgcgtataa aaaatgaata catggttgta aatggatttg cgaattatga tttaacttac
      541 aaagaattta tggagcagtc ttacgttcca cactacaaag ctagcgtaca aaaaagtact
      601 tggagttctc gtgagtgtgg attgattcag attactgaat ttttcggtga taagaagctt
      661 cgtgaaatca atgtgcgaga ttgtgaaaat tatcgaattt ggttattgaa cgatagcgga
      721 tactcacaag catacagttc acttatgtac ggtatgtttc gtaaatcatt agactatgcg
      781 gtcaccctgc aatttctgaa tgagaatatt tccaaaagaa caaaagcaat tccaaaagga
      841 aaatcaatcg ttccttactg gacaaaagaa gaatttgaag aagttttggc aaaaatttat
      901 aaagatgatt tctacgaaca tatgtgtttt ttaatgatct ggctatatta catgactggt
      961 gttcgtgtaa gcgaaggatt agcacttaat tggaatgacg tggacttcaa acgtaaaaag
     1021 ttgagggttc atcacacctt ggaaatgaca aatcaaaaag attttattcg gaaaccttac
     1081 acaaaaactg aaagcggctt gcggatcatt tctctggacg atgatacaat tgaagtgcta
     1141 aaaaagtgga aagttattca aaaaaatcat ggtgtagaac aatttattct tagctacact
     1201 gacctcccac tctatcgttc aactgttcaa cgaattattc agcgttatgc caaactagct
     1261 aaggtacccc taatacaagg aaaaggatta cgtcattctc atgttagcta cctaatcaat
     1321 gagtttaacg cagacattct ggtcgtgtcg caacggttag gccattctag ccctgaaatc
     1381 actttaaagc actacgctca tctatggagc cgtaatgatg aaggaattgc agaacaaatg
     1441 acaggaaaca ttaaatttac ctgtgcaaca aaatctaaag ttgcaaactt taatggaaat
     1501 caagcagtaa aaatctaacc ctaccatttc cctaccaaaa tgaggaatag gctgaaaaag
     1561 cactgatata atagcttctt tacgaggcta ctcatattga gtgggaataa agagccaaca
     1621 taatatggag ggggaatccc tataaatcaa cattcttcaa tagctaaaat gcttgatttt
     1681 cattaaaaat aagatggttc cctaccaaaa gagactataa tataagttaa tttgtttcat
     1741 tcttatcata aaaagaagtt ctcgaaaaac gaactgtttt ccgagaactt ttttctatcc
     1801 atctaagtgc tcaaatcata ttcacgacgg aattctaagg ttaccctttc ttaaacaaac
     1861 tttatgttat ctgcacaact ttttgcaatt ttttcattgc ttcttacgaa atcgttgaac
     1921 tctctttagc ataatacaaa gtgtagatga agtctggtgc tttactcgtt ttggtaaaaa
     1981 tgactggaaa tcgtttctgg ttatttttgg caaaggcgag gtgcaggttc gattcctgcc
     2041 ttctacgcta acgatgtggc tgaagggaac ctccgatctt gaatcgaaac gatccgcagt
     2101 ctattgtttc accaatgaaa caaaatcaac gttcgtccaa gcatttcctg aagccttgct
     2161 ataaacccca atttcacaaa cacctgttgt aattggaatg tctttgattg taatttttgt
     2221 ccattcactt gttttaacaa ccgcagtatt tagttccgct ccaccataat ttttgatata
     2281 taactgatta gtatcaaact ctcccccaga cattacatag gcactcaatg tgtaagttcc
     2341 tttgcttatg ccactaacat tttgataagc tgatgcctcg tatgcttgat cctgccaaaa
     2401 agttagtttg tagtccccaa aaaaagtgcc cgcttccgtt ttgatcgtac ccttagcggt
     2461 tccttgttga aaccaggttt gccaattgga aggactggtc gttggtccat cgctttcgaa
     2521 acttgcattt ttaactagat ttatttgcgc ccctgcttgt ttaaacgcac gcaagcttgg
     2581 caaggcgttt ccagcaaaat caaacatcgc ttggttgtcc cattcgtttc ccatagtgga
     2641 ttgaacactg agataattca tacctgcttg tgttgcccaa ctcacatttc cgttgtacca
     2701 aagaggttcc caatagaaga agcccgcccc gcgattattc ggtactgcct gaatccgatc
     2761 ggcaagatcc agcaaatact ctgcttgtcc tgtcggtgtt ggttcatatc cccccgcagc
     2821 tgcttcggct tcaccaaaag catttggcat agtatcggca ttcgctgttg tatgcccata
     2881 agctgtttca actacgatca cttctttacc ataccgttgg ctgatcgcat tcatattggt
     2941 ttgaagttca ttcattgaac cgtgccaata cggataatac gacactccaa ttaaatcaaa
     3001 tgacacacct cgactcgtga tttcatcaaa ccaccaagta aatgctcctg tatcgccacc
     3061 atcagctaag tggagcatta ctggagttgt tttaggttga gtgtctcgaa cagcttggat
     3121 accagacttc aaaaaagcag ccaatcgatc aaattctccc ccgccttctc cccaagactt
     3181 gccataaggc cagagcattc cgctattcag ttcattgccg atctgaacca tatcaggata
     3241 aacgtccagc tgcttcattt ccgacagaac gttctttgta tagtcgtaca atgccgtatt
     3301 catttcttca aaggtcagac cttgccatgc tttaggaaga ttctgtttcc caggatcaac
     3361 ccaaaaatca ctgtagtgaa aatccagcag caccttaagt cccagatttt ttgctcgttt
     3421 tgcaaggtca atcgtcgttt ccagatcatt tgtccctgct ccataacttt ctccattact
     3481 attataagga tcctgccaca aacgcagtcg aacatagttg acaccattat tttttaaaat
     3541 ctctaaggca tcttttgaaa tatcgtcttc ataatatttt gcaccagatt tttccatatc
     3601 tgacaggatc gaaatatctg ctccgcgaat aaattcgctg gcagatgctt caacagtttt
     3661 tgaaacagcc cccaaagccc ctaacacagc tgcaaataaa acagctttac gcaaccaaat
     3721 tcccattttc attatggctc ctcctttatt gaaattggtt cagaataaac tgaacagctt
     3781 tttcaggatc tctcgttaat ccgatatatt ctgctccttt tgtcgcaacc agcttgattc
     3841 ctaagcgatc ggtgtcttct ttgatatcat catagtaaga attgacttgc ggtgctaaga
     3901 ccaccatatc aaaatcctgc agcatttcgt aatgagaacc ataagcaccc gccattgcac
     3961 tgatcggtgc tttcgattca gatgcacctt tttttaaagc attagctaac atcgcacttg
     4021 tccctgcgcc agcacaaagt accaacacat tgatttcttt atttgcatcg ctgataactt
     4081 gactcgctgc cacaggtact tcctcacgta catcggcaga agcaccttcc tcggttgcga
     4141 tcacctctgc tttttgtgct tctttctcta ctaagacagc atcgtatgct ttgcaaaatg
     4201 gtagatagat caggaagtca acgacgatta gaaccgctgc tagaacaaaa gctaagatac
     4261 ccatgcccgt tcctagaacc aaaccgatcg gtgctggagt tgcccaaggc aaaacgtaca
     4321 taaagctgtt catgcccaat acatcgacaa aaaacttgaa caggcaaacg tttacaacag
     4381 gtgttaacaa aaatggaata aaaaaataag ggttcaaaat catgggtgcc gcaaatagca
     4441 atggttcgtt gacagcgaaa cttactggaa taaaggaagc tttcccaact gccttcaact
     4501 gcttagactt agcaaagagt aggaagataa aaggaacaac caatgttgcc ccagttcctc
     4561 ccatagttcc tacaaaattc cctaaaccca ccgtcagtac tttagaagct tgttcaccgg
     4621 aattgaacaa ttgcagattg gtttccacat tggcatagat aattgccgca attgccggct
     4681 ccacgatcga cggtccatga acaccgacaa accaaaacag cgccatcgct ccccagataa
     4741 tagcgattcc taagtacccg tcagctgctg taaatagcgg ctgcagcacc gtaatgattg
     4801 cttctgcaaa tgagatttgg aagatttgtc gaatgcctaa atcaattaaa acggccgcaa
     4861 atactgaaaa agagaaagga aaaatatctc ggaaggtttg cgaaatcgtt ccaggaactt
     4921 ctttaggcat tttaatggta atatctttca gcacacaaaa cttatataaa ttaactgtta
     4981 aaaatgcagc gacaaaggaa gccagtagtc cctttgtccc catatattcc gttgaaaatc
     5041 caccttcaat cggagagacg cttaacaaca ggaaacttac cacagaggcc agcatcacag
     5101 aggtggtgtt gattactttg tttttcggta agcgacgatt catcgaatca gccaagcagc
     5161 gagcagtggt tgctgaaacg agtaacccta caacacccat cgaataatta tagactttcc
     5221 atagccatgt ggagacagat tcaggccatt ggtaaccaaa aatctcagga accgcagcaa
     5281 tcaaaatgaa aatgcttgaa aataaaatag ctggcatcgc tgttaaaaat ccgtcacgaa
     5341 cggctcctaa ataactatta cgtgagattt tttcaaaaaa tggtttccct ttttcgattt
     5401 gtttaatgat tccatccata gcgttcttct cctatccttg attctgtctg tacagtacaa
     5461 taaagtcttg aatcagatct ctcagcaata aagtggtcat taaatgatcc tgcccatgga
     5521 taaaaatgaa tcccatctcc atctctttcc cagatgcttc ttccgctaaa atttttgttt
     5581 gcgaattatg tgcaagggtt aaattttcgt cggcttcttt caacgccgaa tcaacatgtt
     5641 caaagtttcc ttgtcttact tctttcaata atttcaataa tgtactgcga gcgtcgcctg
     5701 aatatgcaac gatctcaaaa ccaagcattt gtaattcctc tttattcatc ttttcttcct
     5761 ccattatcac ttattaaata atcgttttgg tttcactgac caacttgtac cagtaagctg
     5821 attcttttgg gtaacgcttc tgggtttcaa aatcaacata aaacaagcca taccttttgt
     5881 tatacccatt tgtccatgag aaaagatcca taagcgacca caaaaaatac cccttcacat
     5941 ttaccccggc tgttatcgcc ttgcttaagg attccaaata gactctcaaa tagtcgattc
     6001 tcggctgatc cataataatg ccatcttcaa actgatcttt ataccccatc ccgttctcag
     6061 tgatatagat tttgttgtaa tgtgggtaat cgcttttaat tcgcagcaac aagtcatata
     6121 gaccttctgg ataaatcagc cagtcccaat cagttcgagg aattccttct ttataaatac
     6181 gttctccgat tcccttcact ttataaacag aggtcccttt ttcacccgta ccgttatgat
     6241 ggattgcatt ttctccatcg taagctttga caaaatggca ttggtagtga ttgatcccta
     6301 agtagtcatt acgcgtggat gcttttttca attcaacgaa atcttcttca ggaaaatgat
     6361 agctggcctg atttgcttca catatctcat caagagcagt caaggtttca gttgaataat
     6421 agcctaaata ggtagcatct aataagaaac ggatcgacaa tgcatcgtct aaaaaggcag
     6481 catgcttatc ttccggcgcg tctgttgccg catatttcgt ctctaatgag tgaactacac
     6541 caatttcacc cggaagttcg ttttctttga aatagttcac tacacgggca tgagcaacca
     6601 tcatattatg aagacaggcg acgatctttg taaaatcata tttgattcct ggtgggaaaa
     6661 cacctaacaa atattgattg gttgcaactg gatagatttc attaaaggta ctccacacct
     6721 tgacttcttt gaattcatga aaacaaaaaa tggcatagga aacaaatgct tctattgttt
     6781 ctcgattcaa aaaatctcca tggtcaaaca aacgtttagg ggtatcaaaa tgatgcaacg
     6841 tcacaaacgg cgttacgttt cttttcttac attcttcgaa cacacgatga taaaaagcga
     6901 ttccttctgg atttggtttc ccagctccat caggaaaaat gcggctccaa gcaatggata
     6961 atcttagacc atttacacca aaacgttcac acaattcaat gtcctttgca tattgatgat
     7021 aaaaatcact tgccggatcg ggactgaatc gcccttgttc ctctaaaaag tcatcccaag
     7081 caacagcgcc tttgccacct tccttagtgg ctccttcaac ctgataggct gctgtggctc
     7141 cgccaaaaat aaaatcttct ggtaatttaa gcatgtacat ctctcctcaa atctataatc
     7201 agttttcgct ctttctaact gattgcattg taaacgcatc caaaacattt gtcaacaaaa
     7261 acaaacacaa ataatcataa aaaccagaaa acagcaataa aatctattgt aacaaacata
     7321 aaaaccaccc taaatgttta cttttgttcg aattagattg actgttgtcc gtttatatga
     7381 tagattatcc ttaaattaga ggtgaagaca atgttgaaaa aagaaagatt gcttactatc
     7441 gtagaaatgg tcaataaaaa aggaatcctt accgttaatg aaatcatcaa tcaattagat
     7501 gtttctgata tgaccgttcg cagagattta gatgaattag aaaaatcggg caagctgctg
     7561 cgtgtccatg gaggggctca aagtctttcc tacactttag atcaagaact ttctcacact
     7621 gaaaaagcca ctctgcagat tcaagaaaaa aaagaaattg ctgccacagc agcctccttg
     7681 attcacgagg gagaaacgat tttcttagga ccaggtacaa caatcgaact attggcgacg
     7741 catttactga acaagaaaat tcggatcatt acaaataatt atccagtttt tgatattctt
     7801 gctcaagcag atggaccaga aattctactg attggcggtg atttccggaa aaataccggc
     7861 gcatttgtcg gaccgatcac caacgacaat ttaagaagac taaagtttac aaaagcattt
     7921 atcagttcaa atggcgttca caacgaagcg atctctactt acagtaccga agaaggagaa
     7981 gcacagcaga tcgctctaaa taattcccgt aagaaatatc tactcgttga taataaaaaa
     8041 ttcaatcgag atgattttta tgtgttttat aatttgcatg atttcgatct gctcattacg
     8101 gatcgccaga tctcgcggga ggttcaagag cactatgcac aatatgtcga tctgcaaatt
     8161 gctgattcaa ccgaaaatac cactatagga gcgtaactat gaaagggatt atatttgatt
     8221 tcaacggaac aatgtttttg gactctcatc tgcatgaagc tgcttggctg catatgatac
     8281 atgatcatac aaaagatggt ttatcagacg aggatatttt aaaaaatatt catggtcgga
     8341 cgaatacgga gattttgaaa catttcattt cagagcattt gaccgcctct gagatcgccg
     8401 tattgtcaga agaaaaagaa agctattatc gtatgctttg cttgcaaaat gaaaacgagc
     8461 ttcagttgac aaaagggctt gaagcgactc ttgaccagct gactgaccaa cagattccat
     8521 taacgattgc cacggctact gtcaaagaga atgttgcttt ttattttgat attttcgatt
     8581 tagcccgctg gtttgactgg gacaagattg tctatgacga cggctctttc cctgggaaac
     8641 cgcaaccgga tatttttctt aaagcggcag acaaactgag cttagctccg gctgattgtc
     8701 ttgttatcga agatgcttac tcaggattac ttgctgccaa acgtgcgggg atcggaacaa
     8761 ttattgcaat tgatccattc ggtaaaaaca aaacagtatt tgaacaggca cagctaaata
     8821 aagatggaac catcaaagat tttactggtt tttggacaga gcacctgtct gccacatccg
     8881 ttaattctta aacgattcaa ctaactacca aagaaaagca actgaaatgc acacctcctt
     8941 ttataaaaaa ctgccagtga aacgacatga aagatacact gctatatcct tagtgaaata
     9001 tagcagtgta tctttgactt tttcattctt ttaaccatat gacgcgttca tctctatcta
     9061 taattaacat taaaaatagt gttttcacat tatttctcaa accaatctta aaaatcatca
     9121 ggagtacttt tttgatatac ttttattaat gctgggcgta atggttaaaa gatttccact
     9181 agttcctctc caccaagctg agttgaaaaa tgcttatctg taaacaacat agacaaagag
     9241 gaaataaacg tttccgtatc tttctctgtc ccaaggatat taattaattt tcctactagg
     9301 taagtatcat taaacttaag atctgccttc acatcaaaag ttttgataaa agcttttatc
     9361 atgcgtacaa ttttgtcttt atcgccataa ttgatttcac cactcatatc aatatagtca
     9421 ctaatccctg aagaaataat ggcaactgct tcttctttcg ccttatcaga taattcattt
     9481 aatgactggg attgattgat gacttcttga gttttatctg ttacgtaatc tctcattatc
     9541 ctatcggcat actccgcaaa gtaatcttga aaaaattctt ctacagggta accaacttcg
     9601 ttttcccaac caggagaaat ttcattatca gtacttatta aatctattaa aaaccaaccc
     9661 gttctctgag tgttgattct ataatatttt taatttccta tataaatcaa aaatattata
     9721 gaatcaataa gctaattaag aaacaaactt tagaaactct agaagaactt ctgaatttat
     9781 atagaagtgt gtagctactt actaaatata caaaagttaa aataaaatac tctggatact
     9841 ctttatactg cattaaagtt agtaatatta acagcaaaga tatcgaaaaa acaataacat
     9901 aatatgtgtc aaaatttttc ataataagaa ctcccttact gttgcatact aacataatcc
     9961 aaaaactctt ctacttcagc taaagacatt ccactttctt tagtccaaaa aacagttttt
    10021 tctatcgatc cttctctgta aactgtaaat gtcggagtcg agttaattga gtagcgactc
    10081 ttgaattctt tccactcatt ttcatcttct ctaacttttg ctacattcac gtaataaata
    10141 ttttcttttc ccttttctac cagcatatcg aatagcttcc tgcttttgat gatcgatttc
    10201 ttgtgctaca tctagtaaat tttcatcaat ttctatatca taaagtattt cactagtgtc
    10261 tacatccaca atatatactt tttatcagta gcaatttttg agtaatccgt taaaatgatc
    10321 tcagtctcgt cactttcatc agtataaata tacgttaaca atgaactcac cttttcaatc
    10381 ggtgttagct ctaagcctac tgtatttttt actattgagt cattatttac caccataact
    10441 tcgtactgta tataccatcc tatttggttt ccatcaatat cagtaattgg ctcactagct
    10501 aatggagtct ccggaacaat attttcctga tatttcttaa actcttctgt agcacgaaaa
    10561 aggttatcat gttcctcact tgaaactggt tgagtcatag aaaatgttac ttggctaaac
    10621 agagggaaaa taaacactac aatcaacaaa gtataaaaaa aagcccttct ttttcatttt
    10681 ttatcttcca atcacaaatt gaaaaactta ttcctgcaac tttgagtcta gtatcacaat
    10741 cgactgacta caactattca tttacattc
//
LOCUS       DACDEG010000083        10678 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.087, whole genome shotgun sequence.
ACCESSION   DACDEG010000083 DACDEG010000000
VERSION     DACDEG010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.087"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(198..1046)
                     /locus_tag="IV142_002183"
     CDS             complement(198..1046)
                     /locus_tag="IV142_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8485791.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1210..2112
                     /gene="gnd"
                     /locus_tag="IV142_002184"
     CDS             1210..2112
                     /gene="gnd"
                     /locus_tag="IV142_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP8485792.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2114..3115
                     /locus_tag="IV142_002185"
     CDS             2114..3115
                     /locus_tag="IV142_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP8485793.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3112..3396
                     /locus_tag="IV142_002186"
     CDS             3112..3396
                     /locus_tag="IV142_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8485794.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3429..4226
                     /locus_tag="IV142_002187"
     CDS             3429..4226
                     /locus_tag="IV142_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8485795.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4240..5073
                     /locus_tag="IV142_002188"
     CDS             4240..5073
                     /locus_tag="IV142_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8485796.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5091..5948
                     /locus_tag="IV142_002189"
     CDS             5091..5948
                     /locus_tag="IV142_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8485797.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            5970..6479
                     /locus_tag="IV142_002190"
     CDS             5970..6479
                     /locus_tag="IV142_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8485798.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6516..6917
                     /locus_tag="IV142_002191"
     CDS             6516..6917
                     /locus_tag="IV142_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485799.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7029..7382
                     /locus_tag="IV142_002192"
     CDS             7029..7382
                     /locus_tag="IV142_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485800.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7416..7859
                     /locus_tag="IV142_002193"
     CDS             7416..7859
                     /locus_tag="IV142_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8485801.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7876..8961
                     /gene="uxuA"
                     /locus_tag="IV142_002194"
     CDS             7876..8961
                     /gene="uxuA"
                     /locus_tag="IV142_002194"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8485802.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8954..9667
                     /locus_tag="IV142_002195"
     CDS             8954..9667
                     /locus_tag="IV142_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8485803.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            10419..10619
                     /locus_tag="IV142_002196"
     CDS             10419..10619
                     /locus_tag="IV142_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8485804.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3562 a         1692 c         2316 g         3108 t
ORIGIN      
        1 tactaacttt gttatcgcat ctaacaagta actcgaataa taccaataca accttctcca
       61 aagatgcgat gacatacgtt ctaacccacc gactattgat gttctgtacc acccttgcag
      121 aaccaaaaaa gccactacca aatatttggt agtggctttt tttgttttat ggatttttcg
      181 ctttttctgt tatggtgcta ttctttgatt tcagaaatag tatgtcgaac tttagcgata
      241 gaacgacgtg attctttttc gttgtaaaac atcaaaatca catatagaga gtccaaaatg
      301 cttagttgag aaatacggga agctaaagct tcagatcgga attctgtttc ttcagaaata
      361 gaaataaaaa cgacatcgcc aagtttagca agggaagaat tagcttggct agtaataacg
      421 attgtttttg ctccagtttt tcttacggtt tcagcaatat ggattgtttc ttttgattgc
      481 cctgtgtgag aaatcaaaac agcacagtct ttttccgtca ataaagaagc ttccatcaat
      541 tgaatatggt aatccgtgct gtgagaagtt gcgatagggg aacgcaaaaa tttatgataa
      601 gcgtcagttg ctacaatctc tgaaccgcca acaccgaaaa aatagacacg tttagattca
      661 ttgataatag tagccgcttt ttttagatcc ttttgttgta acaacttttt tgtgtcattt
      721 aaagttgaaa tactagactc gaacaccttt tgcgccatag tgagctcagt atctgttttc
      781 tgaatatttt cgtggataga aatagcagat agatcatttt cttggatcaa catggccatt
      841 ttaaaatctt taaatccatt ataaccaagt ttttttgtaa attgaaaaaa agtagagtca
      901 gcaatgccca gttcacttgc taaattgcta ataggactat gacttacttt gcttggattt
      961 tctaaaatat aatcggcgat agcctgttct tttgggctga agtcgttgta cattgttttg
     1021 atttgtagtc ttatggagac gctcatgaga attcctcatt ttcataataa gattatcatt
     1081 ttttccgttc tcattaatgt taaagataca aggagagtag gaaaaaatca aacaaaaaat
     1141 atttttgtaa aatctattta caaaaaaatt tttttgtata atgagggtat aagaaatgga
     1201 ggcgctatca tggaaattgg tgtaattggt ttgggaaaaa tgggattgaa tttagcttta
     1261 aacttaaaag agcaccatca caaagtacaa ggactcgaca tttcaagtac agctgttagt
     1321 caggcaagag aaaatgacat tcctgcttat caagagatct ctgaatttct agctactttt
     1381 gagcagaaaa aaatcatctg gttaatgctt cctgctggag agataaccga aaacatgtta
     1441 agaaaacttt ttccaaaact cggaccagga gacattatta ttgaaggcgg aaactcttat
     1501 tataaagatt caatccgtcg agcagaagag tttcagaaaa atgaaatcgg ttacttggat
     1561 tgtgggactt ctggaggaat cgaaggagca cgacatcaag cctgcttaat gattggagga
     1621 gataaagaaa cttttctatc cgtggaacaa ttattcaaag atgtagcaat tgaaagagga
     1681 tatctgtatg ctggtgcatc cggaagcgga cacttcttga aaatgatcca taatggaatc
     1741 gaatatggaa tgatgcaagc aatcggtgaa ggattccaac ttgtacaaaa aagtgagtat
     1801 gcttttgact tggaacaagt cgcacgtgtt tggaatcatg gttcggtgat tcgcggctgg
     1861 ctgatggaaa tcatggaaga tcagttaaac gaacatccca atttaagtaa ttatcgggga
     1921 atcgtttctg catcaggtga agcaaaatgg acggttgaaa cagcgctaga aatggctgtt
     1981 ccagttccag taattgcaac tagtttgttc atgaggaatc tttcacagga agaagatagt
     2041 ttttcagcaa aagtagtttc agcactaaga aatgggtttg gcggacatga aatagttgaa
     2101 aagggtgagt aaaatgccag attatcatat agcaatcgtt aattccagca gtttcggaag
     2161 agtctttcca caccacttgg aaagattaaa gtcagttgga gacgttcatt tctttactgt
     2221 tgatcaagag attccaggaa aagaactagc ggagttactt aaaggattca atatcattat
     2281 tgcaagtgtt acgccatttt ttcgacgaga tttttttgaa aacaaagatg aactgctatt
     2341 gatttcccga catggtattg gttacaacaa tattgattta aaagcagcaa aagaacacca
     2401 gacaatcgta tctatcatac ctgctttagt tgaacgaaat gctgtagcag aaaataatat
     2461 aactaatttg ttgtccatgc ttcgacgcac gacacaagca caagcaagag tcaaggacga
     2521 tcgatgggaa gatcgggcgg agttcgtcgg taggacgctg tttaacaaaa cagttggtgt
     2581 gattggtgca ggaaataccg gaagcggagt aatcgaaatt ttgcgtaacg gttttcgctg
     2641 tgatgttcta gcttatgatc cttacaaatc agatttatac atcgaaagct ttggtgcaaa
     2701 aaaagtagaa cttcaagaat tactggaaca atcagatatc atttgtttat gtgcgaattt
     2761 gacagaagag aactatcaca tgatttctga taaagaagtc gatcagatga aagaaaatgt
     2821 ttatctatct aatagtgcaa gaggcgctct agttgatgaa gaagcgatga ttaatggtct
     2881 gaaaagcgga aagatcgcag gatatgccac agatgtttta gaagaagaac cgggacgtca
     2941 agatcatccg tatttagcgt ttgaacaagt agtgattacg ccacatatat cggcctacac
     3001 aagggaatgt ttagaagcaa tgggtgaaaa atgtgtcaga gatgtcgaac agatcagtca
     3061 gcaccttctt cctgaacgtt ctgtccagac aaagagcaac tatataaaag gatgatgaag
     3121 aaaatggtga acggtttaaa agtgaaaaca ggccctcaat tttatttata tgaagaaggt
     3181 ggtatttcaa aagtttctga tctcttgaaa tcatacggcg cgaaaagagt actggtcacc
     3241 cacggaacgg tatcatggga aaaggcgcta cctaaattag tatttttgaa tgacgaaacg
     3301 attcaatttt tttatcatcg ttattctgga gaatgtagtt atgctgaagc cagacgaata
     3361 gctacaatca tcaaaaaaat gaaatcgatt tcgtagttgg cgttggcgga ggaaaactga
     3421 ccgatctagt gggttatagt gccaatcttg caagaatcga tttcggagtc atccctactt
     3481 tagctagtaa ttgtgctcca tggacacctc ttgctgtcat gtataaggag aatggacaag
     3541 cagaagggaa aacagaacac ttttacagac aagctgcttt ttttataaca gatccccaat
     3601 tagtgattga ttcccctgtt ccatatttca ttgccgggtt agcagataca ctagcaaaat
     3661 ggtatgaatc tgagctcatc ttagaacaag attttttgca agatgaatca tttttgaagc
     3721 tagcacaaga tactgcgaag atctgcaaag aagagatttt gaatcattct gctaaagcaa
     3781 tccaagatat ggcacaaaga aaactgacat cggagttcaa acagctttca gagattatct
     3841 ttgctgttgc tggtttagtt ggaggattag gagataaata cgcaagaaat gcggcagcac
     3901 atgctatgca tgacgctatc agtaaatata ttccggaaag ccatcgtttt ctacatggag
     3961 aaaaagtagc gtatgggatt ttctatcaac tggcactaga aggtcgctgg gaaaaaatag
     4021 acgagttata tcctttgtat cgggaattag aactgccggt ttctctagaa caaatgggat
     4081 tatttccgaa agataaacag ataatagata aaatggctga atttattgat tcgaaagaga
     4141 aagtgcattt gattcctata gaggtaacac agaaatcact gatacagtca atgatggaac
     4201 tggaaggtta tatcagaaag atctgaagga ggaggacaca tggaaacatt aagtgtagta
     4261 caaagtttgt tgattgcttt atgggtagcc gcggttatgt cccgctggtt aggtggagga
     4321 gccacgttga ccttacgttt ttctcctttg atgactggat tagtcgtcgg gttgatcatg
     4381 ggggaagttg cacaagcgat gattgttaca gccgctttgc aaatgattta tatgggggtt
     4441 ttttctccag gaggatcaat gccagctgaa ccatctattg cagcagctat cgcagtacca
     4501 gtcgcattat taggggatct gtcgccagaa gcagcgattg ccgttgcggt gccagtcgga
     4561 ttgctcggaa gttacttgta tcagtttcgt tttttcatca atactttttt aggtaaatat
     4621 acagacaaag ctgtagaaga tttgaataca aaagctatgg cccgatcaat tattctttat
     4681 ccaacggttg cctcattttt attattcgtt ccgcttgttt tcattgcttt atatttaggg
     4741 gcgccattga tcgctgatgt aatcaaagct ttagagggaa cagtcgttat ccacattctt
     4801 gaagtagtag gtggagggtt agcggcaatc ggtatcgcga caactgtata cgttatcggt
     4861 cgaaaagatt atctggtctt tttcttcctt gcttatttta tgagtgttgt tttgaaatct
     4921 ttagatatta cgatggttac ttatgccatt tttgggatta ttatcgcttt gatctttgtc
     4981 cagtcgcaaa aagggaaaca agtacagcct gccacaagca ataacaattc agcatctttt
     5041 gatgacgaag atgactatga tgacggattt taatttagga ggaatgggaa atggctgaaa
     5101 taatggataa tcaaacaggt gctcctgaag aaatcacgaa aaaagatgta acaaaagcct
     5161 atctgagatg gcattttgca aacgaaattc ctcactcttt tgaacgttac ttggcgccat
     5221 cactactata cggaatgatg ccaatcttga aaaaacttta taaagatgaa gactctttaa
     5281 aagcagcata taaacggcag ttactgttct tcaacactca attaagctgg ggtggcggtg
     5341 tgattactgg cctaatgtct tctatggaac agcaaagagc agaagaagag tatgaagaaa
     5401 aagagatctt gatgcaggat gacttgatgt ataacacgaa agcgggattg atgggggcac
     5461 tagcaggaat cggagatgca atcgattcag gaaccgtcca atatattttt atcgcaatcg
     5521 cggtaccttg ggcacaacag ggaagtcctt taggcgcact ttttcctttt atctgctttg
     5581 ggttgtatca ggttttacta ggggtatttt tcgcaagaca agctttcact atgggccgta
     5641 atgctacagg cctaatgcaa aacactggtg ttcagacagc gattgaaatg ctatcagttt
     5701 tagggttgtt tatgatgggg gtgcttgcgg gaaactatgt aaaagtagaa tcttcattga
     5761 aatttgcgat atccggacgt gagtttgtta tccaggaaat tcttgatcaa atcgtgccag
     5821 ggatattacc tttagctgtc gtaatgggtg tgtacttgtt ctatacgaaa aaaggattga
     5881 aagtcacaca agcattatta tggctgactg gaatattaat tgtgctagca ggaatcggta
     5941 ttttataaaa agaagggaac gtgaataaga tgggtgtagt caatttagcg cgtgtggatg
     6001 agcgtttgat ccatgggcaa gtaatggtaa cgttatctca aaaaagtgga gtgaattcca
     6061 tttttgttgt agacgaagtt gtagcaaaag ataaatttat gagagatctt tacaaaaatg
     6121 caggaagccg tacaggacaa aaaacaattg tcattacacc agagaaagct aaattctatt
     6181 gggatgaata tcaatttaaa gaatacaatt gtattttgat cgcaaaaaca gtccaaacga
     6241 tttacgactt agtaaaacac ggaatcccca tggaagaatt gaatattgga gggattgctc
     6301 aaaaaaatcc tgatacagat cttttagtga cgaaatctgt ttatttaaat aaatcagatg
     6361 ctgaaaaatt gaaagaaatg aatgaacaat atggcgtaaa gaatatttat tttcaagcca
     6421 ctccatctgc acctaaaaca ggattaaaag atgtattgaa caagtttgat ttgtcataaa
     6481 caaatgctta gaaaaaagat aaagagggcg aaaaaatgaa gaagaacgag ctgtttcgtg
     6541 attgggaatt tcgttatcgc tatatttacc gtaagcgccg tacaaaaaag agtaaacagc
     6601 gttttctttc agcattagta tcagatattt acagtatgag aacagatgtg acagtcattg
     6661 cttacgatac gctagcctac cgatcaaaaa atatttatgt tggagatatc gaaaaagcag
     6721 agaaagttat ctgtacttat tacgatacac ccgttcatgc attaggaagt tatttcatgt
     6781 ttgattggaa agaccaaagg aaaaaaacaa tctactctat attattgtcc ttcattttgt
     6841 tgttcagttt aggttggtgg ggaatgatga tatataacaa aaatcctcat cacgtattcg
     6901 atctgctatc cgtataaaca ggtatcactg tactagcatt tgggagctac tttttcttat
     6961 tgggaaaagc agctcgcgga tggcctagca gacaaacttt tattcgaaat acttcatcca
     7021 tattaacgat gctagaaatg atccgtacga ttgatgatcc gagtgtagcg tatgcgtttg
     7081 ttgatgaagg ctgttatgga gaaaagggct tagacagcgt gagatcgggt atgaaaaaag
     7141 aggctattct tttttatcta gactctgttg gggcagatac accgctacag ttttcgggaa
     7201 attatttttc aaataaagaa cagtggctga aacaagttga taagttgaaa gaaaaaaacg
     7261 tcaattatat ttttagtgca agaaaaaagc aagcgcagtt cttctatttg accaaaacag
     7321 atttacgagg gaaaacattt aattggcaaa atgccaatca gattattgct ctttttcgat
     7381 aagtaaaaag tgcaaaacaa ggagtgagaa gactaatgtt ggaaatcatg atcgttacac
     7441 atggtaagtt gagcgaagga ctgaaagatg cggcggaagt aattgtcggg acatcgagcc
     7501 atattgaaac aatgtgctta gagtctgggg aggacatcca aacattcgga gagaaggtca
     7561 aagaaaaatt aactatgatc aacacagcag aacccatgct agtatttgtt gatctaatta
     7621 gcgcaagtcc gtacaatcaa gcgctcttgg cagtcaatga actgactgaa gaaaagaaag
     7681 cagccactta tgtcataggg ggcgtgaatc tgccaatgtt gttagaagca atcaatcatc
     7741 agatgcttca cacaccgata gaagaagcgg tggaacaaat tgtcaaacaa ggaaaagata
     7801 atttaactgt ttggcatgct tcagcggaag aaaaaaaatt agatgaggac gatttttaga
     7861 aggaaggctg aaaagatgaa gtggggattt agatggtatg gtgaaaaagg ggattcgatc
     7921 ccattaaaat atgttcgcca aataccggga ataaatggga taattggaac tttgctgact
     7981 aaaatgcctg gagatgtttg ggagatttca gaaattgagg agttgaaagc atctgttgag
     8041 agccaaggac tggaactgct tggaattgaa agtgtagcga tccacgatgc aatcaaggca
     8101 gggacagatg aacgagatta ctatattgat aactatatac gaacaatcaa aaacttgtct
     8161 cgatgtgggg ttcgattgat ttgttatagc ttcaaaccaa tttttggatg ggcgaaaact
     8221 gatttgttta atgaaaacaa cgatggaagc tattctcttt tatacgatca gaaaatcgtc
     8281 gatcagatgg agccaggaga aatgtatcaa ttgatacaca gccagtcaaa gggattcaaa
     8341 ttgccgggtt gggaagaaga acggttgaaa aaattcgctc agctggaaaa actgtatgaa
     8401 ggaatgaccg aagaagaatt atttgaaaat ctgacttatt ttttagaaaa agtgattcca
     8461 gtgtgcgaaa aagcagatgt aaaaatggct atccatccag atgatccgcc atgggaactt
     8521 tttggttttc ctagagtgac gaaaaatctt ggcgacttga agaaaattct aaatgcagta
     8581 gattctccat ataatggact aacgctgtgc acaggatctt taggggcaaa tccggaaaac
     8641 gatttagttg aaatcatcca tgaggtaggt tcgcgcatcc attttgtcca cttcagaaac
     8701 gtggagtttt taggagaaag aaagttcaaa gaaacggcac atccctctac agaaggctcc
     8761 cttgatatgt atcatattat gaaagcgttg gtgcaagaag ggttcgacgg tcctatacgc
     8821 cctgatcatg gtagaaccgt gtggggtgaa gtggctatgc ctggatatgg cctgtatgat
     8881 cgtgcaatgg gactcactta catgcaaggt ctatatgagg cgataagtaa ggaaaaacca
     8941 ctgggagagc attatgaata aaatcaagct cttcatcttt gatatggatg gccttttatt
     9001 agaaactgga cgtttagctt atcgtgctta tgtaaaatca gcacaaaagt atgattatga
     9061 aatgcgtaag gaagtctatt atttactaac tggccaaaca gaaatggcga taagagaaca
     9121 aatgggcatt ttgtacggag aagatgttcc atatatcaaa tggcgagaag caatcaatca
     9181 atataaagaa aaaatcgtga aagaagataa gagagtatat acgaaaaaag gagcagaaga
     9241 aattttatct tttgcaaaag aaagaggaat ccatacaatc gtggcttcgt ctaatacacg
     9301 ggtaaaagta gaaatgtatt taaaaatgga aaatctatat gccttgtttg atcaaatcat
     9361 ttcaggggat gatgtaaaaa aaggaaagcc ggaaccagag atctttttga aagcttgttc
     9421 aaaaatgaat attcctccaa gtgaagcact tgtatttgaa gactcgattg cagggatcga
     9481 agcagcaagg cgagcgggca ttttgtcttt tctcatcaaa gatcatttga tagggttgcc
     9541 cgatcataat gggaagcata agttgaaagt agatgtaagt ttatatgaat ccaataaagc
     9601 agactatgaa ttttatgacc tgcatcaagc aaaacgattt ttagaaagta aagggttgta
     9661 tctctaataa agaggactat tttctagtcc tctttattta ttgtcctgca cgactgaggg
     9721 aacagatgaa aaagaagtaa ttttttattt tgaagtatct accttatttg tagtgaacaa
     9781 tttagaacaa acacaaaacg catctactgt tatctaccag tagatgcgtt taaagagaag
     9841 aacgagaatc gtccatatga gggtttccac tccttatgca gattttttta attcttgcac
     9901 tgcttgttcg atcgtaacac ctgtcgctac atgattggta ttttctttgt caaaaacgac
     9961 aaatagattc aaatctgtat ccaaggttac tcgatattgt atatctttgt tatagaatgt
    10021 catagccatt ctcctttctt taaatacttg ctgaaaggca acgaacgcgt tgataacgaa
    10081 attccattta taagtatacc accaattgaa aggttttcat agttaagaaa ttttaaagtc
    10141 aaaagttgca gataaaagca aataaacaag aaaacaaaaa gtgaatcaat cgcggttatt
    10201 caactttttc ttcgattcga agacaataga agacaatgaa ttttatattt cattgcatta
    10261 tccttgtgtt attagaaaaa gcatgatata ttaaatgagt aatctatttt gaaacgtaat
    10321 ttgagcgata tatctacata gataaaactc cttttgccta tcaaatattt attgcaacga
    10381 aaaaacgtac attgatacgt atcaggagga atttatttat gaataacggt acagtaaaat
    10441 ggttcaacgc agacaaaggt tttggcttta tcactggtga agatggaaat gatgtatttg
    10501 cacatttttc agctatccaa ggtgacggct tcaaaacttt agaagaaggc caagcagtgt
    10561 cttacgatgt tgaagatggt caacgtggcc ctcaagcaac aaacattgtt aaagcttaat
    10621 aaaaattgac ttttaaccgt cttttcgaaa gaaaagacgg tttttttgta cgaaaaaa
//
LOCUS       DACDEG010000084        10639 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.088, whole genome shotgun sequence.
ACCESSION   DACDEG010000084 DACDEG010000000
VERSION     DACDEG010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.088"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            23..646
                     /locus_tag="IV142_002197"
     CDS             23..646
                     /locus_tag="IV142_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485805.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1095..2144
                     /locus_tag="IV142_002198"
     CDS             1095..2144
                     /locus_tag="IV142_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8485806.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2141..3022
                     /locus_tag="IV142_002199"
     CDS             2141..3022
                     /locus_tag="IV142_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8485807.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3025..3795
                     /locus_tag="IV142_002200"
     CDS             3025..3795
                     /locus_tag="IV142_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8485808.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4074..4928
                     /gene="aroE"
                     /locus_tag="IV142_002201"
     CDS             4074..4928
                     /gene="aroE"
                     /locus_tag="IV142_002201"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP8485809.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5106..6269
                     /locus_tag="IV142_002202"
     CDS             5106..6269
                     /locus_tag="IV142_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP8485810.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6386..6796
                     /locus_tag="IV142_002203"
     CDS             6386..6796
                     /locus_tag="IV142_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8485811.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6970..9198
                     /gene="topB"
                     /locus_tag="IV142_002204"
     CDS             6970..9198
                     /gene="topB"
                     /locus_tag="IV142_002204"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8485812.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9336..9653
                     /locus_tag="IV142_002205"
     CDS             9336..9653
                     /locus_tag="IV142_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485813.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9813..10049
                     /locus_tag="IV142_002206"
     CDS             9813..10049
                     /locus_tag="IV142_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8485814.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10046..10444
                     /locus_tag="IV142_002207"
     CDS             10046..10444
                     /locus_tag="IV142_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP8485815.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3714 a         1772 c         2254 g         2899 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aattggcgca gctggcgtag ataagttgta ttaatggtga gactttccca
      901 tagatacaac tttttagagt gcatttctaa agcttgtatc tatgctagac atgaacattg
      961 actgccgcat agattagaat ctatgcggtt ttttgtgtaa agacaatgac cgctttggcc
     1021 attgtctttt tttgtagact ggatacttgc ctgattataa taaataaaaa gattgacttt
     1081 gcaggaggaa aaaaatgaaa aacaaacgat taataacagt tgtagtaatt attttagttt
     1141 atttagcagg tgcatttatt tatggaaaag cctcaggcga aaaagaaagt acttcgacga
     1201 accagacaaa aaaaacaaca gttgttgggg tattgcaata cgtgagccat ccggcattag
     1261 atgagattta tcgaggaatc aaagatggtc tgaaagaaga aggattaaaa gaaggagaaa
     1321 atcttgagat caaatttcaa aatggacaag ccgatcagag caagctagcc acaatgagtc
     1381 aacagctagt acaatctgat ccagatgcct tgattggaat cgcaacacct gccgctcaat
     1441 cattagcaaa cacgacttca tcgatcccag ttgtgttagg tgctgtaacg gatcctgtgg
     1501 gggctggatt agtcgcttct ttagagaaac ctggtggtaa tgtaacaggt gtttctgatc
     1561 agccgcctgt tacttcacaa atcaagcttg cttctgattt gttgccaaat gcaaaaaaag
     1621 tcggaatctt gtatgcttct tcagaggata attcaaaata ccaagtagca caagcagaag
     1681 aagctgcaaa aaaagaaggg ttagaatcag taaaatatgc ggtgccttcc actaatgaga
     1741 ttgcacaaac ggttcaagtg atgagccgac aagtcgattt tatctatgtt ccattagata
     1801 ataccattgc caatgcgatg caaacagtgg taaaagaagc aaacaaagca aatatccccg
     1861 tcattccatc tgtggataca atggtggaac aaggtggact agcaacaatc gggatcaatc
     1921 aatatcaatt agggcttcaa agcggtaaga tggcggcaga acttgcgagt ggaaaagaaa
     1981 aaccggaaac gacgcctgtt tatacatttg atcaagggga tacagtgatc aatcaatctc
     2041 aagctgatcg cttaggaatc attgttcccc aatcgatgaa ggaaaaagcc aaaatcatta
     2101 cagatgactc gcagcaagaa acatctaagg aggatgacaa atgatcgtat cagctatttc
     2161 acaaggcatg ctttgggcaa tattaggatt agggattttc atgacttatc ggatcttaga
     2221 ctttcctgat atgacgacag aagggtcctt cccattaggt gctgctgtct gtgtgacagc
     2281 tattactcat ggggtagcgc cgattgtagc tacattatta ggcgtaggtg ctggcatgat
     2341 agcaggactc gttacggggt tactttatac aaaaggaaag ataccggtta ttttagcagg
     2401 gattttagtg atgtcaggat tgaactcagt tattttgttt gtgatgcgaa caccaaatct
     2461 ttctttattg aatcatccga ttctacaaga cagcttccat cgctttgggt tacctgatta
     2521 ctttgacaca gtccttcttg gcttcatctc tttgggcatc ttgattgctg tactgctatt
     2581 cttcttcaat acagatttgg gtcaaggcta tattgcgaca ggagacaacg aaacgatggc
     2641 tcggtcgtta gggataaaaa ccgatcgtat gaaaatttta ggtttaactt tgtcgaacgg
     2701 tgtcattgca ttatccggtg cgctgattgc ccagaacgac ggatatgcag atgtaaataa
     2761 agggattggc gtaatcgtca tcggtcttgc ttccatcatc atcggtgaag tgatcttcca
     2821 agaattgacg ctagcagaaa gattgattgc tattgttgtc ggcagtatca tttatcagct
     2881 attgatttta gtagtaatca aactagggtt tgacacgact tatctgaagt tgttctccgc
     2941 tatcatctta gctatttgtc tgatgatccc gcaattgaag aaagcactga acttaaaaac
     3001 gggttttgag aaggaggcat agtaatgact gatatattga caatcaaaaa tggcagaaaa
     3061 gtcgtaaaca atggaatgaa tgaagagaaa gtactattgg atgaaattaa ccttacaatc
     3121 agagaagggg acttcatcac tgtccttggc gggaatggtg ctggaaaaag cacactgttc
     3181 aatacgatcg ctggtacgct tcaactcagt caaggatcga tctcttttaa agatcagctg
     3241 atcacaaaag attcagaaga aaaaagagca cgattccttt ctcgagtttt ccaagacccc
     3301 aagatgggaa cggctcctcg gatgacagta gcagaaaatt tgctacttgc acaaaaaaga
     3361 ggtcaaaaac ggaaattccg tttacgtaac ttgaaaaatc aaaaagaaga ttttttcaga
     3421 ctatgtcaag aagtcggtaa tggcctagag caacacttgg atactccagc gggtgaatta
     3481 tctggtggtc aacgccaagc attaagcttg ctgatggcga cgatcacagc tcctgagtta
     3541 ctattattag atgaacatac tgcagcttta gatcctaaaa cagctaaagc attaatgcga
     3601 ttaacaaatc agcggatcaa tgaacaaaaa ttgacttgtc tcatgatcac gcatcgaatg
     3661 gaagacgcct tgaaatacgg aaatcgtctg atcgtactcc aaaaagggaa aatcgttcaa
     3721 gatatttctg aaaaagaaaa gcaacatttg acaatgggcg accttttgca gttctttgaa
     3781 gaagtagtgg attgatttcg acagaaaaat tcctttcagt agaaggactt gaaaactcat
     3841 tgacatcttc ctttgattca tcctatactt aaaacaataa acaattaaac atatttgggt
     3901 gttttgtagg atcacgtagt caatgatttt aacaattaaa tagaatattc tttgctctga
     3961 caagtaaacg atgagaaaag aatttgctac tttgccacct agacgtttcg tactttttcg
     4021 tatcaaacgc taagtggctt tttttattga agtaagaaca aaggaggaat aagatgattt
     4081 ctggacaaac caaattagct gggctctttg ctgatccggc ttctcacagt ttatccccat
     4141 tgatgcacaa tactgctttt gaagcaaaca acatcgatgc ggtgtattta gctttccaag
     4201 taaatccaat gaatttgaag caagcagtcg aaagtattcg aacttttgat atgttaggtg
     4261 taaatctttc gatgccgaat aaaacagcgg ttattcctta tctggatgag gtaagccagg
     4321 aagcgcaatt gatcggagca gtgaatacga tcgtgcacga aaagggtcgt ttgattggct
     4381 ataatactga tggtgcgggc ttcatgcaat ccgtgcaaga agctgggatt tcgatcaaaa
     4441 agaaaaaaat gactctactg ggagctggag gtgcagcaaa ggcagtcact gttcaagcag
     4501 cgttagacgg cgctgaggaa atcgtagttt ataaacgaaa caaccaaaca ttcgctcaag
     4561 taaaagaaac attcgaacaa ctcagctatc gaacttcttg taagataaaa gttcgtgact
     4621 ttgccgatac gcatcaatta agaaaagact tgaaagaaag ctgtcttcta gtcaatgcaa
     4681 cagatatcgg gatgggaaag aaaatcaatc agtcaccgct cttagatata agcatgatgc
     4741 atgagaatct agcggttttt gatttgattt atgcacctag agaaacacgt ttattaaaag
     4801 aagcaaaaaa agtaggagca aaaggataca atggacttgg catgttgatc taccaaggag
     4861 caatcgcgtt tgaattatgg acaaaggaaa agatgccgat ccatgtgatt gaaaagctgt
     4921 ttgattaaaa aaggataata gataaagatc ttttattctc gcataaacgg tctcaagaaa
     4981 agtaatcctt tatgtatcga agcatttatt tcgttatttt ttattaaatt atcaaaaaat
     5041 gataggcttt tgtcgattgg ttatcgtatc ctttatttat cagtaagaaa cgaggaggaa
     5101 ccacaatgag tcggatggat cgctataagg acatccataa gaaggcgaaa cctctgaagc
     5161 gggatacggg gagtattttt gctagaagag aacgaaaacg aacgcgtatg gaaaatgagg
     5221 aagatgctac ccgtatttat gaaagagagc agcattcatt tgaacatttg aaccaatacg
     5281 agcaacagat atacagagat gcaccaagag cagaagaaca acaaaaaaat aaaaaatttt
     5341 tcaatcggca aaagaagaac aaaccgttaa aacctcgaag aaagcgatcg tggaaaaaaa
     5401 tcattctatc gattttgctt gttttgatcc tttattcagg tatctcattt tttattggta
     5461 aatatgtagc tgaacatgac acaagcttag aggcagcgac ttcagaaacc tttcatgggc
     5521 aaaaagcgac gaatggtgcg cataatattt tgatccttgg aagtgataca cgcggagaag
     5581 atgccgggcg agccgatacg atcatggtct tacagttgga tggtccttcc cataagccga
     5641 aactgatttc ttttatgcgt gacagcttcg ttacgatccc aggggtagga caaaataaga
     5701 tcaattcagc ttatgcatac ggaggagccg atctagtgcg tcaaacttta gtagaaaatt
     5761 ttggaatcga ttgtcaatac tatgcgaaag tcgatttcaa atcgtttgaa aaagtgatcg
     5821 atgccttatt catgaacgga gtgaagatcg atgcagaaaa agatctgaat ctggatggtg
     5881 tagatattaa aaaagggggc cagaagatgg atggacatgt tcttttgcag tacgcacgtt
     5941 tccgaatgga tgaacaagga gactttggcc gtgtcagacg ccaacagcaa gtaatgaacg
     6001 ctattttcag ccaattgaag aatccattga atctgatccg tgcaccttat gcagctggta
     6061 aagctttagg atatacatcc acagatgtca ctgcttggtt cttgacgaaa aacctctttt
     6121 cgattgcacg aggagctggt ggcattgatc gaataagtgt gccagtagac ggttcgtgga
     6181 attatgggca aagtgattat gctggcagta ttcttgaaat tgataatcaa atgaatcaat
     6241 ctgctattac agattttttg aataaatgat cataaaaaag atcaagaaga agctgcgcaa
     6301 ggcttttgtc atgaatcaat caaacgattc actcgtaaaa aacgctttct tatggtagat
     6361 ttaaccaaga gaggagctgt ttacattgga tatctatctg aacttcaaaa atgagtcgcg
     6421 tgaagccatc gctttttatg aaaaagtttt tcagactgaa gcatcaggta tcaccacata
     6481 cggtgaaatg cctggaatag aacaaatgaa cttgccagaa gaagcaaaaa atctaatcac
     6541 gaatgcacgg ctgaacattt atgatacatt agttatgttt gcagatacgc cagaaggaat
     6601 tggtcctgaa ttagttgtgg gaaataatgt ttctttagtc atccagacaa gaaatgaaga
     6661 agaaacgaag cgcctatttg aaagcttagc tgagggtggt aagattgaga tgccactgga
     6721 tgctgttccg tgggctcagt tgtatgggat ggtaactgat cgatttggca tcaactggca
     6781 gattaaccaa gagtgacagc taaagatgaa tattttctgg aaacatcaag cagcaaacgg
     6841 atcatcgttt gtttgcttga tgtttttgtg tctatttcga ctatacggta tctttttttt
     6901 attgccagat gcgaaaatat gttccataat agagaagtcc gcaaaaaatt aagtaacgga
     6961 ggtcagaaga tgaagacggt gatactagct gaaaaacctt cacaagcaaa agcctatgcc
     7021 gaaaccttcc aacagcaaaa gcggcatgaa gggtattatg aaatcagcga tccattgttt
     7081 gctggtgaag taaccatcac ttatggtttt ggacatttgg tagatatggt tgctccagga
     7141 gcttatgaag agcgttgggc aaaatggtct ttagaaaatt tgcctatttt cccagaaacc
     7201 ttccgttacg aagttccaat ggacaaacgt gcacaatttt cgatcgtaaa acgagaattg
     7261 cagtcagccg ataccatcat tattgcaaca gatggtgatc gagaaggtga agccatcgcg
     7321 tggtcgatta ttcgacaagc aaacgcattt tctaaggaaa agaactattt acgtttatgg
     7381 atcaattctc ttgaaaaaga agcaatctat gaaggtttta agaatctcca acctggaaag
     7441 aactattttc caaaatataa agaagcgcaa gcaaggcaga acgctgattg gctgattgga
     7501 atgaatggaa gtccattata tagcttgcta cttcaacgaa aagggattga tggcagtttt
     7561 tctcttggtc gtgtacaaac accgacactc tatatgattt atcagctgca agaagaaatc
     7621 aaacacttca aaaaagaaac gtactttgaa ggagaaggtt taattacgac aaacaaaggg
     7681 cagttttctg gaaaaatcgt accaaaaaaa gcatttaaaa cccaagaaga gttaacgaag
     7741 gaaatagaaa aacttggagc gcatttaggt aagcaaaacg gacggattct tgaagttacg
     7801 aaaaaggaaa agaggttgca tagtccacgt ttattttcgc tatctagttt gcagacaaag
     7861 atgaatcagc taatgaaagc aagtgccaaa gctacacttg atgctgctca aggattgtac
     7921 gaaagcaagt ttctaagcta tccaagaacg gattcctttt acattacaga aaatgagcat
     7981 cagtatctgg ttaaacatct gcaaaattat aaaaaatttt taggaataga agaagtacag
     8041 acaactgaga tcaaagcaaa gaaacgatat gtcgatgcga aaaaagttca agaacatcat
     8101 gcaattattc cgacaaagac gattccgact cctgcacgct ttgcgaagct ttccaaatta
     8161 cagcaagcaa tctatttgca agttttgcgc actactgtgg ctatgtttgc tgcagattat
     8221 atctacgaag agacgaccgt aatgacgggc gtacagcaat taaagctaaa aagcatcgga
     8281 aagatcccat tacagcaagg ttggcaggaa atcttacgca tcaagtcaaa aaagaaagaa
     8341 gctcagactc ttcctcctat cgtagaaaat gaagaagcag aagtagaact gaaaagtatg
     8401 gagaaagaaa cacaacctcc aaaaccctat accgaaggaa cgttgatcac agcaatgaaa
     8461 actgcgggaa aaacgctgga taatgatgaa gcgcaagaaa ttctcaaaga agtagaagga
     8521 atagggacag aagctacacg agccaatatc attgaaaatc ttaagcagaa gcattacatc
     8581 gaagtcaata aaaacgaaat aactgttacg ccaaaaggaa tcactctttg taaggcagta
     8641 gccaatgaac cattactgac gagcgcagaa atgactgcgc gctgggaagg gtacttgaga
     8701 aaaattggta aacaagaggg gacaccatct gtttttttag agaatatcaa aaaattcatc
     8761 ctacatttat tggcagaagt tcctaatcaa atcgacaagg tagacttttc agatgagatt
     8821 agcgggacac gtcagataaa agcgatggag aagaaaaacg atcaattagg aagatgtcca
     8881 aaatgtaaaa agggaatagt catgctttat cctaaaatcg ctacatgctt gaatccggaa
     8941 tgcgacttca aattgtggcc aacagtagct aaaaagaagc tgactaaaac gaatctcaga
     9001 gatttgcttt caaaaggaaa gacaagtaaa gttgtcaaag gttttactgg taaaaaaggg
     9061 aaattcgatg cagttttagt attaaaagaa gatatgacaa tcggtttttc ttttccatgg
     9121 atcgaaaaca gtggaacaaa agcagttgat ggaaacaaag aaacatcaag aaaaaataat
     9181 gaaaacgaag tagagtaaat gaaaacaatg aaatagcgga atatttattc cgaaaagtga
     9241 gaatataaat accaataaaa ctaaaaaata tcagtgaatc aagcaaaaac gttggggtag
     9301 gctgatttta tatagatagc aaggagtttt tttatttgaa aaaagtgcat tcatttttaa
     9361 attatcttat gggagcaatc acgattgtgg ctgtgtacca agcatttcta aagtatcctg
     9421 attacgggac gaagttgttg tcattgacgc acaatcaaac agcatattca ccaacgttga
     9481 ttttatcgac tttgactgca ggagctaaaa gcgctactta tttttttcat aaaacgacaa
     9541 gcatgctaaa gcgaaagaaa cagcgaaaac aattgttggt ggtggtagcg gctatgatca
     9601 ttatttattt tctaccagtt attacacaag tatttcatca atattttggt taagaatacg
     9661 agagtttgtg acaaaagtca tgtcacaaac ttttttcttt gtcatttttt cgtaagaaaa
     9721 agcaaaataa aagtatatac attgattaaa agtatatact tggatataat gtacttacta
     9781 tagaatgaga atggtataga gggtggcgaa atatgaaaaa aatgaaaaag tataaaatta
     9841 gaaaatctgg caatagcgat gtaacaacta tcccaccaga agtaaaagaa ttcatgggtg
     9901 tgcagacagg tgacgcaatc agttatgttt ttcaatctga tggttctgta cgaatgatca
     9961 aagcacaaga agaacctgat attgattcat tagttgattc gatcatgaat caatatgaag
    10021 acgcgttaaa agatttggtt gatttatgag gtatctttcg actaaagaaa ttatcaaaat
    10081 caacgcgaaa gttattttac gtcactcgcc aggcgaaatg atcggtgtaa aagatgcaaa
    10141 tgcattagat atggccgtca aacagccttc acaagcagta ttcaatcaag aactctatcc
    10201 tgaaatctat gaaaaagcgg cgattttagc aattaattta gctaaaaaac atccttttca
    10261 taatggcaat aaacgaactg ctcttgttgc catgttgttg tttcttcaat tgaataattg
    10321 tccagttgct ttttctcgtc aagaagctgt tgattttatc ataatgatta ccactagttc
    10381 attggatttc gattcactaa agtccaagat aaccagttat ttacgacaaa ccgcaataaa
    10441 atgaacgaat taaaaacttt atttctcaat tagatcagca tgtcattaag gaatatgctg
    10501 atttttttat gtttaatcat acaaaatagt tttttcataa aaatatggaa aatatggatt
    10561 aattttgaaa taattgattt aaaaggaata caaaagagtg aatagtagga aatagtggtt
    10621 tagaactagt gggctgatt
//
LOCUS       DACDEG010000085        10575 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.089, whole genome shotgun sequence.
ACCESSION   DACDEG010000085 DACDEG010000000
VERSION     DACDEG010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.089"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..208)
                     /locus_tag="IV142_002208"
     CDS             complement(<1..208)
                     /locus_tag="IV142_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8485816.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYD"
     gene            complement(226..546)
                     /locus_tag="IV142_002209"
     CDS             complement(226..546)
                     /locus_tag="IV142_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8485817.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            845..1621
                     /locus_tag="IV142_002210"
     CDS             845..1621
                     /locus_tag="IV142_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP8485818.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            1636..1839
                     /locus_tag="IV142_002211"
     CDS             1636..1839
                     /locus_tag="IV142_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8485819.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            1855..2193
                     /locus_tag="IV142_002212"
     CDS             1855..2193
                     /locus_tag="IV142_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAP8485820.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            2180..2359
                     /locus_tag="IV142_002213"
     CDS             2180..2359
                     /locus_tag="IV142_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485821.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(2402..2872)
                     /locus_tag="IV142_002214"
     CDS             complement(2402..2872)
                     /locus_tag="IV142_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485822.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            2959..3657
                     /locus_tag="IV142_002215"
     CDS             2959..3657
                     /locus_tag="IV142_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP8485823.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            3835..4176
                     /locus_tag="IV142_002216"
     CDS             3835..4176
                     /locus_tag="IV142_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485824.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            4169..4840
                     /locus_tag="IV142_002217"
     CDS             4169..4840
                     /locus_tag="IV142_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP8485825.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            4846..5532
                     /locus_tag="IV142_002218"
     CDS             4846..5532
                     /locus_tag="IV142_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485826.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            5535..6284
                     /locus_tag="IV142_002219"
     CDS             5535..6284
                     /locus_tag="IV142_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8485827.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            6296..6565
                     /locus_tag="IV142_002220"
     CDS             6296..6565
                     /locus_tag="IV142_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485828.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            6727..7029
                     /locus_tag="IV142_002221"
     CDS             6727..7029
                     /locus_tag="IV142_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485829.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            7026..7187
                     /locus_tag="IV142_002222"
     CDS             7026..7187
                     /locus_tag="IV142_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8485830.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            7184..7489
                     /locus_tag="IV142_002223"
     CDS             7184..7489
                     /locus_tag="IV142_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485831.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            7489..7845
                     /locus_tag="IV142_002224"
     CDS             7489..7845
                     /locus_tag="IV142_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP8485832.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            7805..8050
                     /locus_tag="IV142_002225"
     CDS             7805..8050
                     /locus_tag="IV142_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485833.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            8047..8466
                     /locus_tag="IV142_002226"
     CDS             8047..8466
                     /locus_tag="IV142_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP8485834.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            8463..9020
                     /locus_tag="IV142_002227"
     CDS             8463..9020
                     /locus_tag="IV142_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP8485835.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            9017..9313
                     /locus_tag="IV142_002228"
     CDS             9017..9313
                     /locus_tag="IV142_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485836.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            9390..9803
                     /locus_tag="IV142_002229"
     CDS             9390..9803
                     /locus_tag="IV142_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP8485837.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            complement(10261..10536)
                     /locus_tag="IV142_002230"
     CDS             complement(10261..10536)
                     /locus_tag="IV142_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485838.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
BASE COUNT         3888 a         1641 c         2174 g         2872 t
ORIGIN      
        1 tatcgtacaa aactgaaaat tcaaaatgag aggcgctgaa atgtgcaagt tcatgagata
       61 aatgaaaggc ttctgcagtt tcaccgtata gattttcatt caaaaaaatg attctgggtt
      121 ttggatagta gaaaccaggc tctttcatct ccatataaac tactttcaaa ttgtattcgc
      181 tcaacatttc tttcaatttc aaatacatac aaaccatcac tccaactatt cattttcctc
      241 taaagcttta gcaattgcaa tcgctttacg cattgtctcc ttagatattt cttttccatc
      301 aaaagaaaaa acagtatcgt cttctgataa atccacatgt ttaggggttt ctctttcttc
      361 tctacctaga aggtagtcta cagagacatc gaaatagtca gcaatccgac ttaattcatc
      421 tgaattagga gtattatttt tccactttgc tagataaccg ttagaatatc caaaattaat
      481 ttctaactgc cgtatagata tctttttcct cttcgccaag tcttttatta tttcataggt
      541 attcatcgat aaaacaacct ttctaaatgt ttacaaaaaa agtttagaaa aatacgcaga
      601 aataatttga ctaattctga gtaatgagct atactatatc ttgtaaacag atttaacaac
      661 taaaaagaca acaaaaaaca ctattgatat gtaaatgcag accgccaaga aagctttaaa
      721 atcaatgttt ttatgtctta tttaattatg ctcttagtat agagtattac tcagatactg
      781 tcaattgaat ttagaaaaaa gttgttaaat ttgtttacga atataaaaga aaggaatgag
      841 aaatatgaac acaccgcaaa tttttaattt cgaacaaaac gaagttcgga caattttagt
      901 aaatgatgaa ccatattttg taggcaaaga cgttgcgagt gttttgggtt attcaaacac
      961 taaagatgct ttgtcgcggc atgtagattt ggaagataag atggggtcgc gaatcacgac
     1021 ctcaggtcaa tcaagagaga tgacaatcat caacgaatct ggtttgtaca gtttaatctt
     1081 aaaatcaaaa cttccctctg ccaaaaaatt taaacgttgg gtaacaagtg aagtgttgcc
     1141 agcaattaga aaacatggag gttatctaac tccagaaaaa gtagaagaag ctttgcttaa
     1201 tccagataca atcattcaat tagcaactca actaaaagaa gaaagaactg gaagattaat
     1261 cgcagaacaa aagattgcag agtacgaacc taaaatctcc tatttagata gcatattatc
     1321 ttctacagat tcagtaacaa ttagtcagat tgcagcagat tatgggatgt ctccacaaca
     1381 gatgaataaa ttacttcata aactaggtat tcagaaaaaa gtcggtaacc aatggttatt
     1441 gtgcaaaaaa cacatgaacc aaggatacac aaaatctcat acaactgaga tcccgaaagc
     1501 cgatggtggc actaaaattg taatgaatac caaatggaca cagaaagggc gtctatttat
     1561 ctacgaatta ctaaaaaaag aaggatatta ccctcaaatg gatttagagg aaattggtta
     1621 gaaaggagtt ttagtatgac tgacattgca gaaatcactc aacgagatag agaaaaaatc
     1681 aaagaatatg tcgaaagttc aaagttctta acttacacca tgcttgctga aagatttgga
     1741 attagcaaaa gctacttatc tttaatttta aacggtaaaa agacttctgc agaagcaaac
     1801 agaattatag attcgattat cactatgtac gaattgtaaa gggaggaaca gctaatgcaa
     1861 tatctagaag caaaaatccc aattccagaa ggctatgtaa ttatctccca agtggattat
     1921 gaggagttaa aaaaagctga tgatactggt agatggatga cgttgccaga agtgctagaa
     1981 cggattaaca gaaaatatga ttggtttact tctagagttt taaagaaccc aagatataga
     2041 aatattatcg atatcgagaa aaacaaaaat ggatttgtct attatccagt tgaaggaaga
     2101 gacacatatc tatttttaag aagtaaaaca cttgaatttt tagaaacaaa tttttcggaa
     2161 atcttaagga ggcaagcgga tgggaaaatt taatagagcg ctagtgttca gcgcaccgct
     2221 aatcatctac gctttaggac tttggggaag caggcaagcg ttgataggaa cgattgttta
     2281 catggtttgg atttttatgg ggcttgatga agctgaagct gagtacagag cgaaaaagcc
     2341 aaccgagggg gctgactaaa aatgaataga aaagagaaac tagaatgtat attactatta
     2401 ctcagtttaa ttctttcact aatttctcta ttgggaagtt tgtatttttg atatcaacaa
     2461 ttttgtggat ttgggttcta tttagagtaa atatcatttt tgtttggtga ttaaggagtt
     2521 tattaaaatt aattttccct acttcaaatg caaggtaaca agatatagca cttttaggag
     2581 gaatatttag cggtacagct tcagagtata ttctattatc atcgactgat cctcctttag
     2641 caattaattt cttaaatgga gtagctgacc atctgcggtt taagttagcg gatgaaagtt
     2701 ctaaattaac taacatagct ggttctgttg aatagttaga aatgattact tttgtataaa
     2761 tcatatctgc agcgaagtaa gaagcattaa gttcaacttc tagttgaggg cgcattcctt
     2821 ttatttttat ccctgttaaa aacgtactca atataaaact tattattgac atccattgaa
     2881 aaattgttaa gtgaaaaaaa ttcaaaataa acaccaccag tttttaacta aattatacca
     2941 aaaaggagag aagaaataat gcaagaatta gtaattttga aaaataaaga agctgtgact
     3001 acgagcttac aagtggcaga aagcttcgag aaaaaacatc aacatgtttt aagagatatt
     3061 gatgcactaa aaaaagatgt gtccaatttt ggacagatgt ttgtagaagg taatgaacca
     3121 gattcatatg gcagaaatcg acgagttttc ttcattagta gagatggatt tttcttgctg
     3181 gctatggggt ttacaggaaa gaaagctatc tacttcaaac aaaaatacat tgaagcattc
     3241 aacgaaatgg aagatgttat tcgcaagaat actgttcctc aaacaattga agatatgatg
     3301 atctatcaat tagaagaaat gaaagatgtt aaaaaagatg tttccatgct taaagatact
     3361 atgcgaatta gcggacaaca agagtttgaa attaagcaaa aaggaaatat gaaagttatg
     3421 ggagttctag ggggaaaaga aagccgagct tatgaagaaa tcagcaaaaa agtattctca
     3481 aaattttggt ctgaatttaa acgtaccttt tcaatcccaa gatatggcga gttacctcgt
     3541 aagagattcg atgatgctgt ttcatttatt gaaatgtggt tgccagaaac tgcgatccgt
     3601 atggaaattg atcaactgaa cagacaacag agactttttg gtgatgacaa tgaatagagc
     3661 tgaagcgcta agaataggga cggcaattgc taattgctgg tggaaatact ataaaccaat
     3721 catcctaagc caacaacata ttgacaagca aaaagcatgg caacaaataa aaaagtgact
     3781 ccgccggcaa gcaaagagtc acaaagaaaa cacatcataa ggagatttta gcatatggaa
     3841 aaagaacttt ccactctaga tcaatatttg attgatcctg attggggcaa gccgaaaatt
     3901 gaggaaacaa gtggtcgaaa aatcagacga aatcttttga cgaatgaaga actagcttgg
     3961 gatcaagatg atttaggcaa ccatgtaact atttgggatc atgtttatct tatccatcta
     4021 tcgaagcatt cgaataaacc tgaatatatt tacgtcatcg aagatggctt gattgatgcg
     4081 ctagaagagt acgacagaga taacttgatt gatatctctt attacggacc aggtaagaaa
     4141 tacattgctg aaatggaggc agaatttgat gagtgaaatc aaagggacaa cgaactttga
     4201 aaaacttttt agtcgtaagt taaataaaat tctcaagaaa aaaggaaatt ttgattattt
     4261 atcttgggct cacgcgtggg agattatgaa aaagaatgat ccacaggcaa cggtaactat
     4321 taatgagtac aaacactaca gggttgtttc tggaactcat caagactttc ttgttgagga
     4381 atataaacct tttcttatgg atgaaactgg gacttatgta tctgtctcag taacggttaa
     4441 aggacacacg gaaaccgaat tatttcctgt tttagattat cgaaaccaac cagttgttaa
     4501 accaaatgca atgcaaatca ataactcatt gaagcgatgc tttgtgaaag cattggctct
     4561 acacggactg ggattatatg tatttcaagg ggaagatatt ccaacaccac ctagaatcga
     4621 tacaaagaaa ttaaacatgc tagagacgat tctagaagct ttcaatgagc agatgggtaa
     4681 agatatgacc aaaaccttaa tcgaatatgt taatgagcag acagataaat tagggctctt
     4741 agctgataac gttgaaacta ttgaacagtt aagctatgag caatgtgcct tgatggagcg
     4801 agcaatagca gctaagagaa aagaattaga taagaagtga tatgagtgtt taaaccatta
     4861 atcgattcat attcagcggt tctgaaaaag ttcaaaggaa aagacataag cgcaaccatc
     4921 aatgaggaag tgaacattga tcgactaaag acgatgtatg acggctacga tggtgatcga
     4981 gtcattgaaa ttcgttttat tgatcctaga cgtttcaccg tacagcaacg aaacttcatc
     5041 tatgcgctga taggcgatat ttttatcgat acaggcatgc caacggactt ctggaaggaa
     5101 ttcttctact ttcgttttga aggtgtcaca gggcgcaaaa taagcctgaa agacgaatcg
     5161 aatacaactg tgagtgatgc caatgtctta gcaaatatca tcttagattt catctttgaa
     5221 catcatattc ctttcaaaga aggctatgag attttacctg cgaatcaaga atattacttc
     5281 tacaaatgca tcacaaaaag agtctgctgc atctgtggca aaacaggagc tgacatcgat
     5341 cactttgaca aagcgctagg aagacgaaag cgcaaagaag ttgatcatgc agagtacaca
     5401 tttgcagcac tctgcagaat ccatcacaca gagaagcaca aaataggtgt gattaatttc
     5461 aaaaataaat atcaaatcaa agggatcaaa ttaaaccaga aaacaatcaa aaagttaaat
     5521 attggagggt aaaaatgaca gaacatcgaa gttattatgc gattatacca gccaacgtaa
     5581 ggtacgacaa aagacttaaa ccaaatacta agttgttata cggagagata acggccttgt
     5641 gtaatgaaag aggcttttgt tgggcaggca atgagtactt tgcagattta tatggtgtga
     5701 ataaagagac catatcgcga tgggtaagtg atttgattaa gtttggatac ttgaatcggg
     5761 aaatcattta caaagagggt accaatcaaa taatcaatag gtacctacga attaatcaat
     5821 accctattga cgaaaaacgc aataccccta ttgacgaaaa agtcaaagat aataatacat
     5881 ctattaataa tacatttaat aatacaaaag aatatataag agagttaccg ccttcgaaaa
     5941 aatcgaaggc taagcccgtc cgtcataaat acggagagta taaaaatgtt cttttgtcag
     6001 atgagcagat ggagaaactc aaaacagaat tccctaatga ttaccaagag cgaatcgaac
     6061 gactgtcaga gtattgtgaa tcatctggta agacttataa aaactatttg gcaactattc
     6121 gaagttgggc aaggaaagaa aaaagtgaac ctaagaacgc aagcagtgga tacaagcgca
     6181 caggaagacg agagaagctt cctgaatggg caatcgacca agaagcctat ctcaagaaaa
     6241 aagcgctaga acgagctaat agacaatcaa aagcaccatt ctaagaggtg gaaaaatgaa
     6301 gatcgattat ctagaactaa ttaatgaaat agcgaattat aaaaagggcg aggaattaga
     6361 cgtcctgaga gacgtatatg atcaactcga agaagctgga attgaaggaa ttaagaatga
     6421 tcgttcgagt tggagtaagc tcagatacta tttcgcactt tatatcgata caacacaatt
     6481 aagaaattta gcatatacca aattactatt tgttgattgt gttaaaggct tgcaaaaaca
     6541 tcttaatgaa cttgagcagg tgtaatcaga tggacctaaa gacatttaca gcacagattg
     6601 aactaatgca tcaagaagct ttaagacaaa gtgtatcgta cgaagacaag tggctcaaca
     6661 cgttccatgg cggacgtgag agcgcacttg atcaagtact caaattactg aaaggagaat
     6721 gtcgggatgg ataagaaagc ggcaatgcag cgaattatcg aattgactta ttcagaagat
     6781 tggcaaaatg acaaagaagc tgcttcagaa gtgatgaggc ttggaagagc gatgtgggca
     6841 gacaagagca acaagccaag accacgaaaa atcgcaattt ggcacggtga caaacttcta
     6901 gtgataggga cagctgaaca gttagcaagt ctcacaggct tgcacgagaa aatcgtgagg
     6961 aaaagagcaa ggtgtggcta cacagacgct aagaagagaa cgtttagata cgtggaggaa
     7021 tcatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     7081 acgatctgga acgaacaaag gcagtgatgg tcaatgaaac ggcgagaaag gcaatcgatt
     7141 tgcacataga gaatttaaga agggaaatcc atcgattgga ggaatgagcg tggataagaa
     7201 agcagcaatg aaaagaattg ctgaattaac caagtcagaa tcttggcaag aagacaaaga
     7261 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg actgaaaagc ccaaacggag
     7321 aacgccgaga aaaattgcaa tctggcatga tgaccgaatt ttagtaacag gtactgctga
     7381 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     7441 atggattgat tcaaaaggac gacagtttag gtatgtggag gagagataat ggatctcatt
     7501 acacaataca gtgacatcat cctcaagaaa atcatgatga agattcagaa agataaaaaa
     7561 tcaaaagaac gagctgaatt agttaagtta gaaatggctg aaacaggagc aggagtgcga
     7621 agtagcaggc attggaaagc agcagcaaac attgaatttt attacaacga aattcaaaaa
     7681 gggttcgatc agatgcgtga gctggatcgg caaacaaatt ggagcaagaa acttcatcaa
     7741 gatcgtttca aatttgtaga gaagtataaa gaaatattag aagagtattt gaggaggaca
     7801 gcaaatgata aaaaaactcg ttcaattcag catggattta tatgatatcg aatcaggagc
     7861 aacactatct gtggaatcgg accatctaat cataaatttt ggtggaaagc gccagattat
     7921 tttgtgggta gttgatgatg tactgtttcc agaaattgtt catgatttcg aagaatcaaa
     7981 agcggttgag tttgaaatag tgaaaaaagt aatggaattg attgaaaaat acgaggagga
     8041 cagcgaatga taccgaagtt tagagcgtgg gataaacgaa agaacgtaat gagagatgta
     8101 gccgtcttgc attttactaa aaacggcaaa acaaacttta ttgaatattg gataaatcct
     8161 accgaattga aatcatatca cgtgcgaaac atcgacctca tgcaatccac aggaatgaaa
     8221 gataagaatg gtgtggatat atttgaaggg gatatagtat tagtcagcgt gcgaaatggc
     8281 ttcgattact tagataataa agtctgtatt gtcaaaaatt caatagatta ttccggatta
     8341 gtttgtgcca ctgttgatga agacttagag tatcaaattt ttaacacaga gctgtttgaa
     8401 gaatacacgt acgaagtcac cggaaatata tacgagaata gcgagttatt ggaggaacag
     8461 cgatgaataa acaggaagca atcaaaaagt tagaaagtat taaagcgata ggaaatgatg
     8521 caatagctgc ttgctataac gagagtataa attcaggtat tacgttaatg aaaaaaatag
     8581 acgaaccgca gaaaccagtt attccacaac tcgtggccgg ttggcttgag aaatctacgg
     8641 acccttttac aaaagctgaa aaaatagcgt atttaatcaa atctaaagat ggtgattcat
     8701 attatttctg tgattggttt gtacgagatg gcatagtgac gcaagagcaa ggagaagaat
     8761 tactcgcttg ggcaacaaga caatcatatg aaacactatt gagcctatac aacggctacg
     8821 aggttgagaa agagccgtta tgggcaataa agaatgccga tggaaactat cttactaaat
     8881 gtgctttatg gggaaaagat ggagtaaatt atagttttga atgcaatcca tctcatcgat
     8941 tgcttttcac tgataaagca acagcggatg ctgcagcatt gttggtgaat ggaacagtgg
     9001 aagaggtggt agaaagatga aactaaaaga cggattttac gctagcagtc acggtatcgg
     9061 cggtttaatg ctagatatgc cgacaaagaa ccctaaaaca cgcaagaaat caaaattcaa
     9121 agtcggtgac atggtccgct gtgaagcaga agagttcgtt tatccgttca gaggatacgt
     9181 taaaaagata ctgtcaaact cagcaatcat tcgtattgaa aatacaatga agtgtgatcg
     9241 ttcgactgca aaacacaagc attatttagc tgtagcgaga ttagttgaca tggaagtaat
     9301 caagagcaaa taaaaaaagc cggatcgctc cgactgattc aataaatcca acacatttat
     9361 tatatcacat aaaggagcgg tttgacttga tgcaattgtt acgagaggta gatttcaaac
     9421 agacaagatg taatgcgaga gatgtgctga agaactttcg gcgtttggag cggatggcag
     9481 gtcgctcttt gatagatatt aagtcgccga ttataacgga tatgccgaag gcaccgaagc
     9541 atggcaataa ggcagaagac gcgattattc agatgatgga tatagaaacg gagagagatg
     9601 cgattctagc ggctttgatg gctcttagtc tgattagtcg tcagatactc tactacagct
     9661 tctgtgtgcc agatagcttc tcaaactaca gaattagccg tgaagtgggt tattcagaaa
     9721 gaagtataca acggatgaag tcggaagctc taatagagtt tgcagaagca tataaacacg
     9781 gaaaaataat tgcttataaa taatttggcg gttttttggc ggaatgatgg cggtttttag
     9841 acatttacca gtgttattat ggtagtgtcg aaagattagg aaacaggatc gacaaaataa
     9901 acgaagggag gcaatctccc tcatcgtttt aaacttcttc ttagatagac agcaactaat
     9961 tatgaagaaa aggatgtgaa ttcaactcct cctaaattct tcatcggttg ctgtttattt
    10021 tgattagaca gcagcactca caaaactaaa catgttcttg tcgtctttca actgctgctg
    10081 tctattaatt tatgtattgg agggaagaaa aatgaaatta tcagtagaag gcactccaga
    10141 agaaatacaa gaattgctcc aagctattgg tagtagcaag gagcaaaagg taagtgtttc
    10201 taattcagat attgacggta tttgcaattg tttaaacaga gaaaaaatat ctaatcctta
    10261 ctaagagaaa atatgttcgt taatgaattt ccaattttcc tctgtagctc taccttgata
    10321 atttttttgc agttcacaga tgaataagga atcgttatta tcaagtactg tatttaattt
    10381 ttcacacatt tgatttggat tcaaattagt tttaactaac caaaaagatt tttgtagctt
    10441 tatatatgcc ccaaattctt ttattatttc aaatacttta tcatactttt gccctgggtt
    10501 attaagatca taactaatca tgtagggttt atacatgttg tcttgcacat ccttgtttta
    10561 tatttcagcg gacca
//
LOCUS       DACDEG010000086        10257 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.090, whole genome shotgun sequence.
ACCESSION   DACDEG010000086 DACDEG010000000
VERSION     DACDEG010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.090"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(935..1600)
                     /locus_tag="IV142_002231"
     CDS             complement(935..1600)
                     /locus_tag="IV142_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8485839.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            1726..3762
                     /locus_tag="IV142_002232"
     CDS             1726..3762
                     /locus_tag="IV142_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP8485840.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            3766..4404
                     /locus_tag="IV142_002233"
     CDS             3766..4404
                     /locus_tag="IV142_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8485841.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4432..4860
                     /locus_tag="IV142_002234"
     CDS             4432..4860
                     /locus_tag="IV142_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485842.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5317..6228
                     /locus_tag="IV142_002235"
     CDS             5317..6228
                     /locus_tag="IV142_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8485843.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            6682..6996
                     /locus_tag="IV142_002236"
     CDS             6682..6996
                     /locus_tag="IV142_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8485844.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            7012..7398
                     /locus_tag="IV142_002237"
     CDS             7012..7398
                     /locus_tag="IV142_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8485845.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            7427..8812
                     /locus_tag="IV142_002238"
     CDS             7427..8812
                     /locus_tag="IV142_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP8485846.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            8815..8967
                     /locus_tag="IV142_002239"
     CDS             8815..8967
                     /locus_tag="IV142_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8485847.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            8990..10195
                     /locus_tag="IV142_002240"
     CDS             8990..10195
                     /locus_tag="IV142_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8485848.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
BASE COUNT         3483 a         1680 c         2003 g         3091 t
ORIGIN      
        1 gccgaaacag tcttggtgtt cgtagctctg cttatatgtt tcgaatttaa gacaaagagt
       61 tggcaaaaga tgttgataag gaagataaga taactgtgtt atataaacta ggtaactaac
      121 tgtaacaagc taattttaag agattcttat tagaagacgt ttaggaatat aactaagaaa
      181 atgattatac aggtaattgt catttcaata atgttttcta attttcgcgt ctttgtttta
      241 agaaaaaggc cagaatcagt tggaaaaaag ttcgtacgat agtgaagagg ggcaaaattt
      301 cctactaata ttcccactaa caagagataa aacggccagg ttggtgtgat tcctctataa
      361 aaaaatgccc atgctaccga aatcccatag attaacaact gaacaatttt tacaatgaaa
      421 aaattgttct gccattactc aaagtctctt cctccttgta tcgtttattt gtttcatttt
      481 atcattatga taggcgcttg aacaatccat tttgtatttt attgagtact cttggataaa
      541 ctaccagcga ttatcatatt tatttatgtt actgtaagcc atcatataaa agtaaatgag
      601 cagatcatag aaatataaaa aagaactaaa attgatctaa attatgtgca gcttgactac
      661 gtaccaatct atattactta ctagattggt cgttaatcta ttcggatcga atgagcaaaa
      721 tatttttgtg aatgtccatc cttgattatg attccaaaaa gaatcattgg gaggctgtgg
      781 cgtaaatatt atactataag ccagaaatac ctatcattct gaactttttt atttaggaac
      841 tttcatctca aggctttttc aattaatttg tacccctgtt ttttggataa gatcatgtgc
      901 attgattttt tctatctcta cgttcatctc ctacttaatg cctatctggc tcctgagggc
      961 ttctttttaa ggcgtcgtca tagtcttggc tgaggatttc ttggtaatct gagggaatgg
     1021 cttcccaagt gagcaggcga acgttcatga cgtgttttcc tttgtgtttg attacagcat
     1081 ggtttttctc tttttctacc tctccagcgt gaaaagacag ccatgtacca tggccaaaga
     1141 tatcattaca tacccaacgt tcgatggaat tgtccacgta tcctacaggg agtgtggtat
     1201 agccgatttg tggtttcaaa aaaggattga atcgatcgat cgtttcattt ttgtattgat
     1261 aattgataag aaaaagatag accaaacaaa agacgagcag aaagaaagat agtgtctttt
     1321 tgagtgagaa gggttgtttt gatgaataag atttcttgtt aacaagaggt tcatctggca
     1381 atgttggttc tattgattcg gtagctgatt caatagaagg aaaatcaatc ggcagaccta
     1441 aagtatgatg taaaagtgac agcatttcaa ggtctggcat tgtacgagaa ttttcccaac
     1501 tagaaatcgt tttatcggaa acaccaattt tttcgcctaa ttctttttgc gtaagcttac
     1561 aatttctgcg gtaagttcgt attttttctc cggtgttcat cttcttctcc tttcgattct
     1621 acagtatata gatttattct acatgaccta tacacttgat agatacgttt ttttgatgaa
     1681 aaacggtatg attttatcaa agaatcattt aagtaaggga gagtggtgat aatcaaaaaa
     1741 aagttcacag ttttactcac aatcatagtg accggtatca tgagtggttt tgccttggtt
     1801 agtataaata aacaggtact ctatcggaca aatgaatttt tccatcaaac agatacttat
     1861 actccgattc gattgccaca acattttatt gggcaagaag aatatgagca gctaatcatg
     1921 gtatttgaaa atgtctcatc agaaactgag ctttcttata tgaaaaaaac agtaaatgaa
     1981 tccaatgaag tagatacatc ttggaacttc aagatacagc caaagcttga aatgacattg
     2041 tattatcaag gggatgtcaa atcttctcac gagaaggtac cctattctaa tacctccttt
     2101 cgcgtaacgt ccattgaaga atcgatcaaa ataaaagatt ttgaaggtga atttttcttg
     2161 atgacgagag aaaaagaagc ctatgaacgg ttcatttcac tattcactga tcgattcaat
     2221 caagcatttc acactgatta tgccactaat atttttgaag actttgaaaa tagctattcg
     2281 tattcggtta tttcaaatac agacgtatat aattatcgag tgtatttaac tattggattg
     2341 attcttttat tccttttgtt atcatccttt ttcttattga tgaataaaga aatcagttta
     2401 ttgttgaata atgacttttc attacatcaa gtattgtggt gtttattggg gaaaaagaaa
     2461 atcattatta cattcataat tgcaggaatc attggtggtt ttatttttat gtattataag
     2521 gagcaatttc tttatttgtt ccagatgtac gttttcttgt tcagcggaat atatggtatc
     2581 tctttgattt ctcttttatt agttgaaaaa atcgcttcgt taagagaagc gttcaactat
     2641 atccaataca ttctttttac atttggattg ttgttttctc ttcttttgac cgttgccaat
     2701 gtcgaattag caactatcgt tttttcttct tcacagttag ctgtttccca tgaaccacca
     2761 aaagaaatcg ctggtgagga ctatcatgta ttttatccag taaccatcgg gaaaaaccac
     2821 gtggatttta tttattccaa tcgttttgca tcagcagcag atcaggaatt atatgaaact
     2881 ttgaataaaa acggaagtat attagtcaat gtatcagatt acttgaatga agaaaatgaa
     2941 cagtttggca gagggataaa aataaatctg aattatctta agaaatttcc tttgattgat
     3001 gtctctggaa ggttgatcca aataaccgaa aaagaaacgc accctgttat attgatccct
     3061 gaacgatata gacatacaga tttaaaaaat gacctggcgc aaataacaga atattatcaa
     3121 gaaatcagtg aaaaagaacc gaaatttcta tttataaaaa acaatcaacc catttataca
     3181 tttattcctt ctatcccctg gatcgaacat tatccggttg ttgaaatact gacattgaaa
     3241 aacagtatgt atttggaaag aaatattctc agcggagaaa tctatccacc gttaaagatc
     3301 aagataggct ctttgtcgaa agaaaggtta tttgagctga ttgaagaaag tcagttacgt
     3361 gataacttgc agttatcatt tccgtatact cagactgaag agatagctgt caaagtgtta
     3421 agcagatcat ttcattactt gatacagata tttttactca ctttcttttc tttcttcttg
     3481 ttgagctatg tcatgttatg catatatttt gtctacaatt gtcggaaaat cgctatcttt
     3541 cgttcaagtg gttattcgct ctttgaaacg tataaagatt tcttcatgat gaacctcatc
     3601 aaatggggaa cgacaagtgt aatcttcctt tttcttatag aaagagagcc taaatatctt
     3661 tttaatattt ttttctttag cttcattgat tttattcttt cagttgtttt tattttgtct
     3721 acagaaaaga aaagtcagtt attgctgatg aatggaggtt aacaattgga gccgttgata
     3781 gtgataaaag gtgcgaaaaa aatcattcga aacaaaacga ttcttcattt ggatcgtttc
     3841 actgcggatg ctggggatct tgtgactgtt gttggagaaa gtggagcagg taaaagtacg
     3901 ttattgaata tattaggatt gaatgatcag ttcagttcag gacaattcta tttgttcgat
     3961 caagagacga aacaattaac tgggagagag aagcgcttga taaaaagaaa taccatatct
     4021 tttctttttc aagattttgg gttgattgaa tatgaaacaa tcgattttaa tttagatatt
     4081 ggattgaagt tttcgaattt aaatgtttca gataagcgaa cagcaaagta tgaggcattg
     4141 aaaaaagtaa atataaacaa gaagctaagt acgcctatct acacattatc tggtggtgag
     4201 aagcaaagaa tcgcctgtgc gagagtctta ttgaagaata gcaaaatcat tttagccgat
     4261 gaaccgactg ggtcattgga taaaagaaat aaggataaag tagttgaaat cttatcgcaa
     4321 caagcttccc aaggtacgtt agttatcgta gtcacgcatg atccatatct gaccaaaata
     4381 ggaaacaaga agatcatgct ttaaaaatca aaaaggaaag gatgtcgttt tatgaaaaaa
     4441 gcagttatta tttgtgcact agggataggt tttgttggag gaaacgctat gtcatctgaa
     4501 gtggaagcaa attcagttca cggtgaaatc tattacaaat ctttaaagcc aggagagatt
     4561 cattttgatg ctaaaggtaa tattattaag tcaaatttac cagtagaccg ggaaatttct
     4621 agaacagcaa ttaatcatag ctctggaaga tgggtttatt atagtgatac aacttgtacg
     4681 agaaaaacag gtcattctaa ccactatagt cgtgtctata atcgtcacgg tgcaaaagcg
     4741 aaagttggaa actctattcg atcagcaaat gcaacaaaag gagtatgggc ctatgcaagt
     4801 gcgacaggaa atagaactga cacattctca tgttggtaca atccaaccgg ttggtattaa
     4861 gtatccaaaa gtttatataa aatagaaaaa acatataatg aaacagcgtt aaaagttatt
     4921 tttggtagtt ttaatatatt catttttagt tgtataaaaa tatatcataa taaaaacaac
     4981 aaaaaaatgt ctggaaaatc gaacataaaa gtgtctgatt tgaatatccc agcagcggaa
     5041 gaagaaaaac aaaatcgata acatttgttc taagaattag ttacgttaaa tacggaacgc
     5101 aagatagact tgaaaaacgg taacaaaaga agtagtatat taaggtgtga tgtgctactt
     5161 gacatttatt ctgctgagcg aactttctgg attgtgtagc cttatatcta ttcaggacgt
     5221 atcgagcaga ttagtaaagg tgttcttgat gattttcatc ttgaacacct ttgtttttag
     5281 caattcgcaa aaaacgaaaa gtaggagaat tgataaatga aaaaaagatt tttagcactt
     5341 gccatcgtac taggcactgg cttattgtca ggctgtacga atgccggtga aaaaacagcc
     5401 gttagttata aaggcggtac aatcagcgaa caagaagtaa tggatagctt gaaaaaaatg
     5461 caaggagcag attccgctgt gcaacagttg atcgtttatc aagtatttga agataaatat
     5521 ggagatgacg tatcaaccaa agaaatcgat tctcaatatg atcaaacaaa gaaacaattg
     5581 ggtgattcat ttgattcaca attgaaatct gcaggttaca cagaacaaac attcaaagac
     5641 agtatcaaac aaagtctagc ctttcaagaa ggtctaaaga aacatatcaa attgaccgat
     5701 gaagatttaa aaacggcttg ggaatctttc catccagaag tggaagctca aattatccaa
     5761 gtagcaagtg aagatgatgc aaaagatgtg aagaaagctg ctgataaggg agacgatttc
     5821 tctaaattag ccaaagacaa gtcaactgat actacgacaa aagaagatgg cggaaaagtg
     5881 aaatttgatt ccactactac aacggttccg gcagaagtca aagaagcagc ctttaagttg
     5941 aaagatggtc aagtttctga tgtgatcact tctaccaatg cttcaactta tactacggaa
     6001 tattatgtgg ttaaaatggt gaaaaaccaa aacaaaggca acgacatgga taaatacaaa
     6061 aaagaattga aagaaattgc aacagacaca aaattaagtg acagtacctt ccagaataaa
     6121 gtaattggcg aagtattgaa agacgctaat gtaaaaatca aagacaaaga ctttgaaaat
     6181 gttttgagta cattcacttc agattctagt acagcaagta gtaaataagc ggtataaata
     6241 aaaaatatga actatttaaa tcaataaaac tgttcatcta aatcttttta ttagaactaa
     6301 atcaacagta gtttaattac tacttgattt agttcttttt ctacaaaaaa tagcataaaa
     6361 atctagttat ccgcataaaa actggactta tcacacttta tcaaggtcaa aaccactcaa
     6421 tttactacta atttactact tatgaatgag ctttgatacg acgatttatc cttgaaaagt
     6481 gaagatataa agatacttcc aataaaattt gaatatttaa taggtagaca cttcaaaaaa
     6541 tgaggtgtct atttttttac ccgattttga aaggaagtga acttatgaaa acaaaaaatc
     6601 aagaatcaaa aggtcgttcc ccactcttta agaccatcaa acattcattc agccaataaa
     6661 aaagaaagga taggtaaaaa tatggaactt aaatttgtga ttcccaacat ggaaaaaaca
     6721 ttcggcaatt tagaatttgc tggcgaggat aaagtcgttc agcgaagaat caacggacgg
     6781 ctaactgtct tatcaagaag ctataatctc tattctgatg ttcaaagagc agatgatatt
     6841 gtggtggtgc ttcctgctga agctggcgaa aaacatttcg gctttgagga acgtgtgaag
     6901 ttagtcaatc cacgtattac cgcagagggc tacaaaatcg gcactcgtgg ttttacaaat
     6961 taccttttac atgctgacga catgataaaa gaataaagaa agagaggaaa aatgatgaga
     7021 ttagcaaatg gcattgtatt agataaagac acgacttttg gagaattgaa attctctgct
     7081 ctacgtcgtg aagtgagaat ccaaaatgaa gacgggtcgg tttcagatga aatcaaggaa
     7141 cgtacctatg acttaaaatc caaaggacaa ggacgcatga ttcaagtaag tattcctgcc
     7201 agcgtgcctt tgaaagagtt tgattataac gcacgggtgg aacttatcaa tcccattgcg
     7261 gacaccgttg ctactgccac ctatcaagga gcagatgttg actggtatat caaggcagac
     7321 gatattgtgc tgacaaagga ttctagttca ttcaaagctc aaccacaagc aaagaaagaa
     7381 ccgacacaag acaaatagtc gctaggtaga aaggagactt tttcgcatga aacagcgtgg
     7441 taaaaggatt cgcccatctg gtaaagattt agtctttcat tttacgatag cgtcactcct
     7501 gcctgttttc ctgctggttg tcggactgtt tcatgtgaag acaatccagc agatcaactg
     7561 gcaggatttt aacctatcac aagcagataa gattgacatt ccctatttaa ttatcagttt
     7621 cagtgtcgca attcttatct gcttgctggt agcgtttgta ttcaaacggg ttcgctatga
     7681 tacggttaaa caactttacc accgtcaaaa actggcaaag atgatacttg aaaacaagtg
     7741 gtatgaatct gaacaggtca aaacagaggg tttctttaaa gatagtgctg gtcgtacaaa
     7801 ggaaaagata acctacttcc ctaaaatgta ttatcgactt aaaaatggct tgatacagat
     7861 acgggtggaa atcacgctgg gaaaatatca agaccaactc ttacacttgg aaaagaaatt
     7921 agagagtggc ttgtactgtg agctgacgga taaagagtta aaggattcct atgtggaata
     7981 tactttgctc tatgacacca tagccagtcg tatttctatt gatgaagtag aagctaaaga
     8041 tggtaaactt cgcttaatga aaaacgtatg gtgggaatat gataagctcc ctcatatgtt
     8101 gattgctggt ggtacaggtg gcggtaaaac ttactttata ctgacactga ttgaagcctt
     8161 gcttcataca gattcaaaac tgtatattct tgacccgaaa aatgctgacc ttgcggactt
     8221 aggttctgtg atggcaaatg tctactatag aaaagaagac ttgctttctt gcattgaaac
     8281 attctatgaa gaaatgatga aacgtagtga ggaaatgaag cagatgaaga actataagac
     8341 tggcaaaaat tatgcttact taggtctccc ggcacacttc ttaatctttg atgaatacgt
     8401 cgctttcatg gaaatgctgg gaacaaaaga aaacaccgca gttatgaata agctgaaaca
     8461 gattgtcatg ttaggtcgtc aagctggctt ctttctaata ctggcttgtc aacgtccaga
     8521 cgcaaaatat ttaggcgacg gaatccgtga tcagtttaat ttcagagtgg ctttaggtcg
     8581 tatgtctgaa atgggctatg gcatgatgtt tggcagtgac gtacaaaagg atttcttctt
     8641 aaagcgaatc aaaggtcgtg gctatgttga tgtaggaaca agtgtcatat cagagtttta
     8701 tactcccctt gtaccaaaag gatatgattt cttggaggaa attaaaaagt tatccaacag
     8761 cagacagtcc acgcaggcga cgtgcgaagc ggaagtcgca ggtgtggact gatcttgctg
     8821 gctggtgtgg caatagccac gccagcactt aaccccccgt atctaacagg ggggtacaaa
     8881 tcgacaggaa acagtcaaaa aaacattaga aaatcctttg gttacaaggg atttacaaaa
     8941 tttcagcgta tgtcaaatgg gctttaaaag ttgacatacg cctttttgat tggagggatt
     9001 tttactgaat gaacaaactt ggttacagca tttaaaagaa aaacgcttgg cttatggact
     9061 atctcaaaac cgtttagctg ttgcgactgg tattacaagg cagtatctaa gcgatattga
     9121 aacaggaaaa gtcaagccat cagaggattt acagcagtcc ctttgggaag ctctggaacg
     9181 cttcaatccc gacgctcccc ttgaaatgct gtttgattat gtaaggattc gctttccgac
     9241 aacagacgta cagcaggtgg tcgaaaacat cttacaactg aaactgtcct attttcttca
     9301 tgaggactat ggtttctatt cttattcaga gcattatgct ttaggcgaca tattcgtcct
     9361 ttgctcccat gaactggaca aaggagttct ggtggaattg aaaggtcgtg ggtgcagaca
     9421 atttgaaagc tatcttctgg cacaacaaag aagctggtat gagttcttta tggacgtttt
     9481 ggtggctggc ggtgtgatga aacgccttga ccttgccatt aacgataaga cagggatttt
     9541 gaatatccct gtactcactg aaaagtgcca acaggaagaa tgtatctccg tcttccgcag
     9601 ttttaaaagc tatcgcagtg gcgaactggt acgcaaagag gaaaaggaat gtatgggaaa
     9661 caccctctat atcggttcat tacaaagtga agtttatttc tgtatctatg aaaaggacta
     9721 cgagcagtac aagaaaaatg atattcccat tgaagacgca gaagtaaaaa accgttttga
     9781 gattcgattg aaaaatgagc gtgcctatta tgcagtccgt gatttactcg tctatgacaa
     9841 tccagagcat accgccttta aaattatcaa tcggtatatc cgttttgtag ataaagacga
     9901 ttccaaacct cgttctgatt ggaaactgaa tgaagaatgg gcttggttta ttgggaacaa
     9961 tcgtgaacga ttaaaactaa ccacaaaacc agagccttac tccttccaaa ggacgctgaa
    10021 ctggctatct catcaagttg ccccgacctt aaaggttgcg attaaacttg atgaaatcaa
    10081 ccagacgcag gttgtaaaag acattctcga ccatgcgaaa ctgacagacc gacacaagca
    10141 gattttgaag caacagtcag taaaagaaca ggacgtgata acaacaaaaa aataactcaa
    10201 atacaaattc attgaatata gagaggagaa catttttatg aattttggac aaaacct
//
LOCUS       DACDEG010000087        10234 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.091, whole genome shotgun sequence.
ACCESSION   DACDEG010000087 DACDEG010000000
VERSION     DACDEG010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.091"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            117..266
                     /locus_tag="IV142_002241"
     CDS             117..266
                     /locus_tag="IV142_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP8485849.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            277..1791
                     /gene="dltA"
                     /locus_tag="IV142_002242"
     CDS             277..1791
                     /gene="dltA"
                     /locus_tag="IV142_002242"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP8485850.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1788..2993
                     /gene="dltB"
                     /locus_tag="IV142_002243"
     CDS             1788..2993
                     /gene="dltB"
                     /locus_tag="IV142_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP8485851.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3038..3271
                     /gene="dltC"
                     /locus_tag="IV142_002244"
     CDS             3038..3271
                     /gene="dltC"
                     /locus_tag="IV142_002244"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP8485852.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3274..4542
                     /gene="dltD"
                     /locus_tag="IV142_002245"
     CDS             3274..4542
                     /gene="dltD"
                     /locus_tag="IV142_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP8485853.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4683..5765
                     /locus_tag="IV142_002246"
     CDS             4683..5765
                     /locus_tag="IV142_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP8485854.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5765..6568
                     /locus_tag="IV142_002247"
     CDS             5765..6568
                     /locus_tag="IV142_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP8485855.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6638..7378
                     /locus_tag="IV142_002248"
     CDS             6638..7378
                     /locus_tag="IV142_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8485856.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7389..8393
                     /locus_tag="IV142_002249"
     CDS             7389..8393
                     /locus_tag="IV142_002249"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP8485857.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8438..10201)
                     /locus_tag="IV142_002250"
     CDS             complement(8438..10201)
                     /locus_tag="IV142_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485858.1"
                     /translation="MLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDS
                     KYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEES
                     NPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQV
                     LTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEP
                     FVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAEL
                     PELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGI
                     YQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQH
                     IQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLI
                     LARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKI
                     YLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQ
                     EKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3284 a         1699 c         2075 g         3176 t
ORIGIN      
        1 ataaaagcta tgtgacaaaa ctgttagtaa aaaaacggtt tctgtaagct agacacatgt
       61 ttctttttgt caaaattagt tacactttgt gtgaggttat atgaggaggg aatccaatga
      121 acaaatggcg ttctaatcca aaattgaatt attggggaaa attcatcatt cgaacaattt
      181 tttatagtgc agtactgctg ttcctgattt atctgtatca ctataaaaat attcaaggag
      241 gaacttttat ctataacgaa ttttaggagg aagagtatgg aaatcaaaac gattattgaa
      301 gcaatcgatg attggggaat aaatgaacct gatcgagttg cttatcaggc ggatgagacg
      361 catacttttg gtgaattgaa acaagcatca gacgcacttg cttattattt agaaaaaaag
      421 gtagaaggcg atggaccaat tgtcgttttt ggcaatttgg agtttgaaat gattgtctca
      481 tttctagggg ttgtcaaatc tggccatgcc tatttgccga ttgaagagca tacacccaag
      541 gagcgtatcc tatcgatctt tcgggtggct aaaccatcta tggtcatcag tatcggggac
      601 tggatagaag atattccttc ggttcctgtc ataacaaaaa aagaatttca agaaatcaag
      661 aagataccag taggatttta tcccagaaat agcgtaaaag gtacagaaaa tttttatatt
      721 atttttactt ctggtactac tggagaacca aaaggcgtac aaatcagtca tgataatcta
      781 gtcagctttg ttcaatggac gatggaagac tttgggatca agcctggtat gcatttctta
      841 gcccaagcgc cattttcttt tgatttatcg gtattttcta tttatcctgc attagtttct
      901 ggcggaatgc tgaagccgtt aaataaagca gttgttcaag actttcgtca attattcgcg
      961 acattgccag ccttgaagtt gaatgtgtgg gtttctactc catcatttat ggatatttgc
     1021 ttgatggaac ctacattcaa tggagaaaat gtgccggaat tggacatgtt tttattttgc
     1081 ggagaagaat tgacgaaaaa aactgcagaa agtctacttg atcgattccc aaatgcacgg
     1141 atatataata cttacggtcc aacagaagca actgtagcta tttcaagtat tcagattgac
     1201 cagcatgtac tagatagtta tgaccgacta ccgattggct acgtaaaaaa agacacgaaa
     1261 gtttctattg tccaagaagg taaacctgtt tcaaaaggag aaacgggtga aatcattatt
     1321 gcaggaccaa gcgtatcaaa aggttacctg aacaatcccg aaaagacagc tgctgcgttc
     1381 ttttgtcaaa atgacttagc aagttaccat acaggggatg caggtagact agatgaatat
     1441 gggcttttat tttatgaagg acgtatggat cagcaggtca aattacatgg ataccggatt
     1501 gaactcgggg atatcgaaca ttatctgctc caagacaacc gaatcaaaca agctgtcgtc
     1561 gtaccaaaat accaaggaac aaaagtacag cagctggttg cttttgtcgt attagaaaac
     1621 aaaacgaaac agccggattt tcagttgact aaatctatca aagaacaatt actagaagtt
     1681 gtaatggatt atatgattcc tcagaagttc cattatgtcg atctgctgcc tcagacagtt
     1741 aatggaaaaa tcgatcgaaa aaaattaaca gctgaggtga atcctgaatg atgttacctc
     1801 atatgatccc ttatgcagag ccgttttatt tttttctttt agccattgcg cttgtaccga
     1861 tcgttctatc tttattgata tgggaaaaaa gattaccagt ttatcaatca gcagtgacac
     1921 ttttcttttt atttgtcagc tttggaggta attattggca acagggagcg gcgctgattg
     1981 ggtatgtttt gtggcagacg atacttgtat ggggctattt tgtttatcgg caaaggaaaa
     2041 atcagacagg atggtttttt gccgccgtta ttttagcgat aattccactg tttttaacga
     2101 aagtgactcc tttttttgaa catggaaaag catcgttact tggtttttta ggaatctctt
     2161 atttaacctt taaatctgta caaatgatca tggaaataag agatggcatg atcaaaacat
     2221 ataagccatt tcgttatatc cagtttctgc tgttctttcc aacaatctct tctggaccaa
     2281 tcgatcgcta taggagattt gaaaaagatc agctccatcc gcctgttcgt gataaatatg
     2341 ttgatctttt gggaaaaggc gtccaaaata tttttgtcgg ttttttatat aaattcatta
     2401 tcggttatta ttttggtcaa gtactgcttc ctgtagtggg acgcattgcc ttacagcatg
     2461 gtggtatttc atgggcatta gtaggttata tgtacgtgta cagtatgtat ttgttttttg
     2521 attttgctgg ctatagctta tttgcagtcg gtacaagcta catgatggga tatcagacac
     2581 caatcaattt caataaacct tttttatcat ggaatatcaa agaattctgg aatcgttggc
     2641 atatgacgct gtccttttgg ttccgtgatt atgtatatat gcgattgatg ttcttcatga
     2701 tgaagaaaaa attttttaaa agcaagatcg taatgtcaaa tatcggttat tttgctttgt
     2761 ttcttctaat gggtatatgg catggattga cgtggtacta catcgtatat ggactgtatc
     2821 atgcttgtct gatctgtctg acagatgcat ggatccgatt taagaagaag cataaagagc
     2881 agttgccttc taacaaattt acgcatggct tagccgtatt tttgacattt caagcagtat
     2941 gttttagctt cctgattttt tcaggatttt tagataaact tttttttagc taacgaatag
     3001 tgacatatta atttatataa caaaggagaa aaggaaaatg gaagaacaaa ttttatcaat
     3061 cttagaagaa ataacaggaa cagatgaagt aagaaaaaat cgtgatattg atctatttga
     3121 tgaagggttg cttgattctt tagcatctgt tcaattatta gtcgagctag acggagagct
     3181 ggatatccaa gtccctgtat ctgaattcga gcgagaggat tggagcacac cgaataaaat
     3241 cattcaacaa gcaactgcat tgaagggttg agtatgaaaa agaaactttt tgctattttc
     3301 ggcccggttg ttttagctgt tggcttactg tttgtgtttt ttacctcctc ctatcaagta
     3361 gatatctcta atccagaaac gattagacgt gcctcttctt ccatgtcagt gaacgtgttg
     3421 aaaggagatg cgataaaaaa ttatgcatta tctgaaaaac aatatattcc tttttttggc
     3481 tcaagtgaat taagcagaat cagtccattt catccgtctg ttctggcaga aaaataccag
     3541 cgaaactatc gtccattttt gctgggagca cccgggacac aatcgttaag ccaatatatg
     3601 atgatgcgtt ctgcaggaga tgctatgaaa aacaagaaag tcgtctttat tatttctcct
     3661 cagtggttcg ttaaaaatgg tgtaaaaact gattacttta atacgtatta ttctgaactt
     3721 caaacatacg actggttgtt ttcaatgaag aaagtaacgc ctgctgatcg ctatttagct
     3781 agaagattat taactttctc aaaagtaaaa gagaatgata cgttgacggc gattctgcaa
     3841 acgatcaaaa aagggaagct acctttacct gaatctttga atcagctgcg cagccaatgg
     3901 aacatgctga agagagagga tgaagtattc agcaatattg ggttgaaaga tcgacagcaa
     3961 aaaatcgatc atgagtcaaa acgattacca aaacaatacc aagaaacaga attatccatt
     4021 ttagcaaatc aaattggaga acgagaaacg acgaataatc cattcgggtt gaaaaacgat
     4081 ttttatacgc atcgtatccg agcccacgag ccagagctca aacagtcaca aaaaaattgg
     4141 gattatcgtt tttcgccaga attttctgat tttcagctgg tcttggatca attagcaaaa
     4201 aaccataacg aagtattgtt catcatccca ccggttaacg agaaatggag cgattatacc
     4261 ggactatctc aagaaatgtt acaaggattt gccaaaaaga tcaaatttca attaaatagc
     4321 caaggattca atcgaatagc tgattttgtc aatcaagcgg gtaccaacta ttttatggaa
     4381 gacacgatcc atctaggctg gaaaggctgg ttggctgcag atcaacagat cagaccgttt
     4441 ttagaagaaa atcatattac tgctagtaag tatcatcttg acgatgcttt ttttagcaaa
     4501 tcttggcaac atcaaatacc agataaacta caattaaaat aaagaaaaca ggcgagtgtt
     4561 gctcgcttgt tttctttatt tttgaatatt ctgaataaaa gataaaaata ccaagtttgc
     4621 ctgaacaaaa atcataagca atggtataat cacaatatga atatagaaat gggggaaaac
     4681 atttggatac aaaagaagaa cagctactta ttgatttaac ttctgcaaaa ggagtacctg
     4741 gaaatgaaga agaggtaaga gaagtattca gagaatatgc caaaccattc gctgatgata
     4801 ttttttatga cggtttaggc agtgtgattg ccaaacatgg tgctggcgga ggccccaagg
     4861 tctttatttc tggacatatg gacgaagttg gttttatggt aacaaaaata accgaaaaag
     4921 gattcttgga attccaaact cttggaggtt ggtgggggca agtcatgctt gctcagcaag
     4981 ttgaaatcaa aacacgtgaa gggaaaatcg tccacggtgt gattggttcc aaaccaccac
     5041 atgttttaac acctcaagta agaaataaac cttatgaaat aaaggatatg ttcattgata
     5101 ttggcgcatc aagccaagaa gaagccaaag agtggggcat acgtcctgga gatatggtca
     5161 ctccatatat tgaatacaaa cgaatgaatg gcagtaaata tttactggca aaagcatggg
     5221 ataatcgtat cgggacagct gtttctctgc gagtattaga gaatctatct aaagaagcac
     5281 acccaaatgt tttattcgca ggaagcgatg tgcaagaaga agtaggcttg cgaggtgctc
     5341 gaacaagtac acaccttgta aatcccgata ttgcttttgc tttagatact ggtacagcag
     5401 gagacactcc aggaatgaca ccaaaagaag cagattcgat tttaggaaaa ggcccacaga
     5461 ttttgatttt tgatgcatct atgatcccgc ataaaaaatt attgaatttt gttattgatg
     5521 tagcagagga aatggatatt ccattccaat atacagtgat ttctggcgga ggaactgatg
     5581 caggacaaat gcatttgaca agagatggtg ttccgtctgt agccattacc gttcctgttc
     5641 gttatctgca ttcccataca tcagtgattc atgaagatga ttatttgaat acagtgaaac
     5701 tagtcacaga attagtgaaa cggcttgata gtgaaacagt tgaaaaaatt cggagttatg
     5761 aataatgaat aatagaatac ctatttctga atatcggaaa aaagcacgag tttctttgga
     5821 aaatcaatgg ggaatcaata cttggcttgt tttcttatct ataatattta cgagcgtcat
     5881 ccagaacttt tttgacggtt tcagtccttt tccggaagaa agcatgcagc agcacttatt
     5941 gagttttcta gtcgataatt tacttttatt cgcattcaac tatgcattgt tttacattgc
     6001 gctagtagtt gtacggggtg gcagaccaaa aagtaacctt ttattttcag ttttccaaag
     6061 aaagtatttt ggttcgatac ttggcatcaa tttgatcaat tcagtcatca actggctact
     6121 caacagttta atctttctgc ccatgttttt aatcagcgga gtgagtactt atatgcagct
     6181 gcttttcagt tcaaatgcgg tacctacggg tgtcatgcca gaagattttc ttgatatcag
     6241 ctttgtttta tcgatgatag ggatgatttt tgtttcagca ctcatcatga gtattgttgg
     6301 cggtttattc caatttgctg catggacaaa gttcgactat cctgatttgt cggttattca
     6361 atgtttgaaa tatggctggt ttttattgaa agatagaatt ggtacgtata ttttgttgca
     6421 gctgtctttt atcggctggt atttgctagg agcaatcgct ttgttcatcg gtcttttgtg
     6481 ggtcattgtt tatgtgaatg tgtcagttgc agaattttat gaacaagcac ggattgaaaa
     6541 aggaggtcca gacgcctatt ttacttagca tgtaagcagt ttattcatca aaaaaataac
     6601 ttttgtggta ggataaatct ttgaggggga atgtacgatg aaattaactg ttttaggatg
     6661 cttaggagcg tatccttata aaaatcaagg gacaacaggt tatttgcttc aatctgacgg
     6721 gttcaatttg ttgatcgatg caggtagtgc tacgctgatc aaattacaag aacaccttga
     6781 tccacttgat ctagatgctg tgatccttag ccattatcat catgatcata ttgctgacct
     6841 tggtgtatta cagtattact ggcaacttca tcctaagaga aatccggaga cattgctttc
     6901 aatttatgga cacacaaaag attccattca ctttgaagaa ttgacgttgg aaaatgtctc
     6961 aaaaggacat ccttattttg aagctgaaga attaaaaatt ggtccttttc tgattacttt
     7021 tatggaaaca atccatccag tagtatgtta cgccatgcgt atcgtagaag aaacaacggg
     7081 gaaagtattc gtatttacag gtgattcagg ttatttggaa agtttcgctg attttgcgaa
     7141 agatgctgat ttgtttctag cagataccta tctttttgaa ggaaatgaac atcatcatgc
     7201 ccatttcact tcaaaagaag ctggagaact tgcgaaatca gctaacgtaa agaaacttgt
     7261 acttacacac ctacctcagt ttggtgattt agaacaattg aaaaatgaag cgcaaaaggc
     7321 agcaggcaaa gggattgata tagaacttgc tgcagttgac aaagtgttta acatttagga
     7381 ggaagaacat gatttttgta ccaaatgaaa accacgaccc aagaatcaat ttagcaattg
     7441 aacaatattt gctgcaagaa atgcctttag atgaacctat tttgctcttt tatatcaatg
     7501 aaccttcaat catcatcggc agaaaccaaa atacgcttga agaaatcaat cgagattatg
     7561 tggaagaaaa tggcattcat gttgtccgtc gcttaagtgg aggcggagct gtctaccatg
     7621 attttggaaa cctgaacttt agttttatca tgcctgatga tggggattca ttccgtgact
     7681 ttgcgaaagt aacacagcca atcatccaag cacttcatga attgggtgtt gctggggcgg
     7741 aattgaaagg ccgaaatgat ctagtcattg atggcatgaa attttctggc aacgcgatgt
     7801 atgctaccaa cggtcggatg tttgctcatg gaacaattat gttcgactct gatatcaatg
     7861 aagtagtcaa tgcattaaaa gtaaaaaaag acaaaatcga atcaaaagga atcaaatcga
     7921 tccgttctcg tgtaacgaat atcaaaccat ttcttccaga agaaaagcaa aaaatgacaa
     7981 cagaagaatt tcgtcaagaa atcttgttga aaattttcgg cgtagagtca gtagaagaag
     8041 taaaaacgta cgaattgact gatgaagatt ggaaaaaaat caatcgaatc tctgaggaat
     8101 attatcgaaa ttgggattgg aattacggga agtctccaga tttcaatttc tctcgccaaa
     8161 aacgtttttc tatcggttcg attgaagtcc atttgaatgt ctcagaagga atgatcaaag
     8221 aagcaaaaat ctttggcgat ttcttcggac taggagatat ctctgacgtg gaaaaaaaat
     8281 tggttggaca gaaatatgat aaagcatctc tatcaaaaat agtcagccaa attgatgtga
     8341 agaaatattt tggtgcgata gaaccagaag aattgcttac cttactttat tgaaaataaa
     8401 aaatagcgga atccacatat atggattccg ctatttttta tacaggattc gggtaatttc
     8461 gagagagttg aacagtgatc cgatcaatga aagtatcttt ttcctgcgtt agtgttcctg
     8521 tgtaacgatc cgctacgtct ttcatcatca ttaagtcaaa acgttcctga tcatctaatt
     8581 cttctgtgat gcctcgacta aataggtttg tatcgtaatg tgcactatgt tcgcaacaaa
     8641 tgacgaaatg ttcctcttgt tcgtgtatcg acaaataaat ttttcgatac agtggatttt
     8701 cgatggcatg agtttctcta aaggaatgct ctaaagcgtg gatcaaaagt tgagccagat
     8761 cgtcatcgtt gataggaagt tcttcgggaa gatctgcttg gaaggttatc tgaatattcc
     8821 gtttggcagc taacttctgg taacgcgcta agataagatt tgtcacctgg tggttgcaaa
     8881 aagaacacag catttcgaaa ttttttgctt cttctaatat cttttccaca tagtttttag
     8941 cttttgtttg gtccgtatgc attaaatctt gcagattctc catctcttgg acaaattcca
     9001 ggcgaagtgt atttacttct tggatatgct gtcgaagctg ctcataatgt tgctccaaca
     9061 aatcattttt gatttttaag aaatctactt gtctccgatc tttgtcggta tttaagatgt
     9121 gatcgattag attgtagctg ataataacga ttagaatgat aatatataaa atcgtagacc
     9181 aattgatcgt tgtttgatag atacctaatg ccgtttggat gtaaatcaag caatggataa
     9241 tggctaccaa gacgatccaa ggtgtacaag ctacataaaa acggttattc ttatatgctt
     9301 cgccaatgca tgcaagtgaa gtgactaaag tgcttataac actagcaata ttagcaatcg
     9361 gcattaattc aggaagttct gcaggagtaa aagttgcata aagcagaacg ccgataaaaa
     9421 tcaaaagttg gaacgtacaa aaaattccgt accatttttt atagtaaaac attcggctcc
     9481 aatagtagca gatgatgaaa gctgacgtta agataataaa taaatgagat aaaaaagaat
     9541 gaacgaatgg ttcaaaaaga ataccactta aaatatcttc tgatacagct tcaaatccta
     9601 atgccaaagc aaagcaactt aagagaataa gtgagtattc aagacgtttt tgtttataca
     9661 tcaagtagat cgtatagaaa aaggtcccta tagcgataaa aacagcgatt atcatcgtca
     9721 atacttgcgg cactgattgt gaataaataa aactaatcaa tggtccagtt gttcctagat
     9781 aaactttcgg tggcagtcca gcatagttct gataaggaga tgagactgta agggtcaatt
     9841 tttttccgat ataatttgct ggtaaatcta tcacagccaa tgagagtccg ggattgcttt
     9901 cttcctgata atctttccgt tggtataaaa tcttttcgcc tagatataca gtaatccatt
     9961 gatggtttcc tttgattagt aaatttgctt cgtccaacgg tttttcaagt gtcgtttcca
    10021 taatgaatgt ttcgtcttta tccacggtag gaatcgtacg agtgtacttg ctgtcaaact
    10081 taaaatcagg atcatctttt gtatagaaaa cccacgaatc ttttatttct ttcatttgat
    10141 tgctgtccgc aggagaaaaa ataaaataaa agaaataagc aattcctgtt aaaaataaca
    10201 atgataatat aatgattatg ctccacttta tacg
//
LOCUS       DACDEG010000088        10120 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.092, whole genome shotgun sequence.
ACCESSION   DACDEG010000088 DACDEG010000000
VERSION     DACDEG010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.092"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            109..615
                     /locus_tag="IV142_002251"
     CDS             109..615
                     /locus_tag="IV142_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP8485859.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            632..1531
                     /locus_tag="IV142_002252"
     CDS             632..1531
                     /locus_tag="IV142_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP8485860.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1618..2502
                     /locus_tag="IV142_002253"
     CDS             1618..2502
                     /locus_tag="IV142_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP8485861.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2694..2765)
                     /locus_tag="IV142_002254"
     tRNA            complement(2694..2765)
                     /locus_tag="IV142_002254"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2730..2732),aa:Arg,seq:ccg)
     gene            2825..4159
                     /gene="rpoN"
                     /locus_tag="IV142_002255"
     CDS             2825..4159
                     /gene="rpoN"
                     /locus_tag="IV142_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP8485862.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4362..5399
                     /locus_tag="IV142_002256"
     CDS             4362..5399
                     /locus_tag="IV142_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP8485863.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            5440..6441
                     /gene="gap"
                     /locus_tag="IV142_002257"
     CDS             5440..6441
                     /gene="gap"
                     /locus_tag="IV142_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP8485864.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6551..7741
                     /locus_tag="IV142_002258"
     CDS             6551..7741
                     /locus_tag="IV142_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP8485865.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7845..8600
                     /locus_tag="IV142_002259"
     CDS             7845..8600
                     /locus_tag="IV142_002259"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP8485866.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8716..10014
                     /gene="eno"
                     /locus_tag="IV142_002260"
     CDS             8716..10014
                     /gene="eno"
                     /locus_tag="IV142_002260"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP8485867.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3546 a         1865 c         2098 g         2611 t
ORIGIN      
        1 cttctttttt gtatataaaa atcccaagta aatacataaa aaataaaaaa attgaaactt
       61 ttagcgcacc ttgtacgtca tattaagtga ggcagaaagg agtggagaat gatttttact
      121 ggatataaga aaaaacggca gatcgaaaaa taccgtcacc tgctgaatgg actcatcgaa
      181 actgattatc aaaaaatgtt ccgtatcacg atgaattacg tacatgataa agaagaggcc
      241 ttagatgtga tgcaagatag ttttcataaa gccctgaact attttgaaaa agaaaaagag
      301 atcgagcatt ttagtgcatg gttttatcgg atattgatcc gcacggcatt agatagttgg
      361 cgcagacaaa aaaggacgcc tgtggactta tttgaaattc aagatatccc ccaacagtta
      421 cctttagaaa cttctgttgc agagctccat atgattttag ataaaataga cagtccggaa
      481 aaagagattc tgatcctcta cttttttgaa ggatttcgtt tgaaagaaat tgcgactatc
      541 ttggatatga atgaaaacac agttaaaacg aaaatgtacc gctcattgaa tgctttacgt
      601 caaatcctag aatgaaggag agaagagtgc catgtattcc caaaaagaaa atttaaaaaa
      661 actaaagaaa acttataaag aacaagcaat tcccaaaggg cttaaagagg agatccgcca
      721 gcgttttatt atcgaagagc aatcatttat aaaagcaaag cggagaaaga gacgtctcca
      781 agggttcgga gtatctttgg ctatcgtaat gattacaaca agtagccttc tttttaatag
      841 tcaagttcga agctttgcag aagatttacc catcttagga tcagtcattg aattgatctt
      901 aggcgaacgc tttactgatc gatctgaaaa aatcgatatt caggtaccga agatcaatac
      961 acaaaatgag aaagaaaata aaacaattca tggattaaat caaaaatact ttcgcgaagg
     1021 acaagcagat tttgaaaaag ctgaaaaaga atatggaaat tttgaaacgg atcattacca
     1081 agttttggga gattatcaga aaatcgtaga cgataaccgg tttctcgtga ttgaaaggca
     1141 gatcacccaa acagcagcag atagccatgt tgaaaaaaga tacgacacaa tcgacaagaa
     1201 aaacagtgtc cagctctcac ttccactttt attcaaagat gacacctatc tatctgtttt
     1261 gacaaaagaa gtcaaacggc agatggccga acaagtcaag gaagatccat ctaagtacta
     1321 ttggactgag caagatattc aagaagggac gatagaaaaa ccaacacttg ttacgcctac
     1381 acgaagtttt tatttgaaca aagagcatca attagttttg actttttctc aatacgaaat
     1441 tgctcctggt tatatgggta caccagaatt tgtcattcct aagtccgtga caaaaacgat
     1501 tttggcatcg gaagattatt tggatcatta gacgagaagt agttgcgact agcagtttca
     1561 attttcaagt aagtacaagt aaataataag aagagaaaaa gagaaagaag agatatagtg
     1621 aaaagaaatt atttagttat cagtattatt gctttatccg tgcttttgtt tttcgaaatt
     1681 acttcggtgg cctccttgat caccgaagta aaaggacaaa cacactcttc ggagcattcg
     1741 tcacaatctt tcaaacaatc agaacaatca accattaaaa gccaatcaac tgaaacagct
     1801 aacgtcaaag aagccattca agaatcagcg gaaaaggtac aaagtcagtc atcacaatca
     1861 acacaagaca gtacaaaaac gagccgagat ccggctgtca aaagcgtcgc aatcagcttt
     1921 gatgatggac caggagcgac cacaacaccg cagcttctgc gaatcttaaa agaaaaaaac
     1981 gtgcatgcca cttttttcgt cttaggagaa aatacggcgc agcatcctga aatcgtgaag
     2041 cagacagctg aagcagggca cgagattggc aatcacacat acgatcatca gaatctggcc
     2101 actctttctg cccagtcgat gacggaggaa gtgacgaaag cagacactga gatcaaaaaa
     2161 gtcactggca aaacccccac ttttgttcgt ccgccctatg gttctgtcac gaatgtagga
     2221 gcaacgatca tccagcgacc gatcatcgaa tggtctgttg attcagaaga ttggaagaca
     2281 agaaatcctg atttgatttt gcagaagatc caagcaacag tatacgatgg ggcaattatt
     2341 ttattccatg atatctatcc tgaaacgatt cgtgccgttc ctcaagtgat cgattatctg
     2401 aaagagcaag ggtatcgcat cacgacagtt ggggatttat tagggcatcc aacggctgtc
     2461 gagaactatt atggcagaaa cgatcatcgg ccagttcagt aagtgagaca agtatcttta
     2521 ataaataaca cattttaata agtagttgaa cacataacta ccttacttgc aaaggcttaa
     2581 caatatctat acaggtacaa caaaaaactg tccaccaatt ttctttggcg gacagttttc
     2641 acttaaaaat aaagaagcaa ctattcgtaa attatgctaa aatagcagta gaacgcgtcc
     2701 tgagggaatc gaacccccgt ctcaagaacc ggaatcttac gtgatatcca ctacactaag
     2761 gacgcaagca ctgggataat tgtaactaaa cagataaaaa attacaaggg gattggacaa
     2821 aaaaatgaaa tttgagcaaa atttttcaca aaaacagcag cagacacaga aactagccat
     2881 gacgcaacag ttgcaacagt ccatccaagt tttacaatat aacagtgagg agctgttggc
     2941 attcgttgag aatcaagcaa tggaaaatcc attggtggaa gttgttgagc cggaatggca
     3001 gccagattac ataaaagctt cttcatcttc ttatgaagga gaagagacaa attacctaaa
     3061 tcagattcct gatacaaaag ggtctttatt tgattcattg atcgaacaag tccatttgaa
     3121 ttaccgagat acttttttga ggaaaatcgt tctttatctt gtagaataca tcgatttgaa
     3181 cggatttttg acgattagtt tagaagaagc cggaaaagag attggagcta cgcctatcca
     3241 aatgctcgat gcgttgactc tgattcagca attagaacca gctggagtag gtgcacgtag
     3301 tttgcaagaa tgtctcatgc ttcaaacaga acgagatgat tatgcgccgg aattagctta
     3361 tatcgtgttg gaagaatgtt ttgaggaact ggtggagcga aaatggaaag aaatcgcaca
     3421 acgtttttct gttgatctac atgctgttca gcagatattc gactatttac aaacgttgag
     3481 tccaagccct ggccggattt tcgaccgtag cagcgaattg tacattattc ctgatgtccg
     3541 ggtattagtg gacgacgata aaaatgtaca agttatctca aaccgaaaaa atcagccaaa
     3601 tatacgcttt caagagagtt acttcaaaca aatgtcacaa caagcagata aagaaactga
     3661 aaattattta aaagaacgga aacaggaatt cgaatggttg aagaaaacga tcctacaacg
     3721 tggggataca attttgcggg ttgctcaagt cattgtttcc cggcaaaaag ctttctttat
     3781 cgacaaggaa cgtccaatca agcctcttac gttaaaggaa gttgcaactg agatagatgt
     3841 gcatgaatcc acggtcagtc gtgcagtcaa cgggaaatat ttggaaacag acttcggtgt
     3901 atttgaatta aaaaaatttt tcactacacg aattcctacg aatagtacag aacagacaga
     3961 agacctttca gcagatacag cgaagaaaaa gctgcaggaa ttagtcgatc aagaagacaa
     4021 aaacaagccg ttgtctgacc agaaattggt tgaattgctg aaaaaagatg agatagcgat
     4081 ctcaagacga acagtagcaa aatatcgtga tttattagga atcccgagtt catcaaaaag
     4141 aaaacgttat gataactaat cagtcaaaag actagaaaaa gtatttcact ttttctagtc
     4201 tttttagttg aaaaacgaga gcaaaactgt tagacttaca gtcgtgaggt tgagatttcc
     4261 ctttattgat gtgaaattcg aatagttaat ttcttttttt atcgtatatg ggtcgtttta
     4321 agtctacatt ggacgtaaag tgtccaacta aggagatcgc tatgctggat gaaataaaaa
     4381 tgattgaatc tgttgcacca gatattatcg aagtgttgca agaacgctat aaaattttac
     4441 gaaatatcta ttggatgcag ccgattggac gtcgaagttt atcggaaagt atggggatca
     4501 cagaacgagt attgcgaaca gaaacagatc ttttgaaaaa tctgaatttg atcgacacct
     4561 caaaaagcgg tatgacgctg acaactaagg gcgaagaagt ttatcaaagc cttgaaaact
     4621 tcatggatca gcttttaggt acgcatcaaa ccgaacaaca gttggcagaa tattttggta
     4681 accaacgctg tatcgttgtt tcaggaaaca gcgaagagca gaccaaagtg gctgatgcat
     4741 tcggcgaagc gctgagcgaa gccctagacc gactacttcc agaaggcgag aatatcatcg
     4801 ctgtgatggg aggaactact atggctgtgg tagccgaaca attgtctaat ctggaaaata
     4861 agaaacggca taacctgttt gtccctgcaa gaggcgggat tggtgaagcg ataactgtac
     4921 aggcaaattc tgtcagtgca agaatggctg ctaaagcaaa cggtaaccac cgtgcacttt
     4981 atgttccgga acaattaagt ctggcaactt acaattcttt actcaatgag ccatctatcc
     5041 aagaagtctt aaacttgatt agtgaagcga attgtgttat tcatagtatt gggcgtgcat
     5101 tacacatggc agcacgtcgt aagatgactg aaaaagaatt agtcatgctg aaacaagcaa
     5161 atgctgttgc ggaatctttt ggctactttt tcaatgaaaa aggtgaagtc gtttataagg
     5221 tccctagaat cggcatagaa ttgcaagatt tggaaaaagt cccgatcatc atggcaatcg
     5281 cgggcggaaa atcaaaagct aaagcaattc gagcttatat gaaaaatgca ccaaaacaaa
     5341 cgtggctcat cactgatgaa gctgctgcaa atgagatttt aaaaggggta accctttaaa
     5401 ataaatattt ttttgatttt cataaggagg aaatcttaaa tgacagttaa agtaggtatt
     5461 aatggttttg gacgtattgg acgtctagca ttccgtcgta tccaagatgt agatggaatc
     5521 gaagtagtag caatcaacga cttgacagat gctaaaatgt tggcacactt gttaaaatac
     5581 gacacaactc aaggacgttt caacggaaca gttgaagttc atgaaggttc attcaacgta
     5641 aacggaaaag aagttaaagt attagctaac cgcaacccag aagaattacc atggggcgaa
     5701 ctaggcgtag atatcgttct agaatgtact ggtttcttca cttctaaatc agcagctgaa
     5761 aaacatttaa cagctggtgc taaacgcgtt gttatctctg ctcctggcgg aaacgatgtt
     5821 ccaacaatcg tttataacac aaaccacgaa acattaactg gtaaagaaac agttatctca
     5881 ggtgcttcat gtactactaa ctgtttagct cctatggcta aaacattgaa cgacaaattt
     5941 ggtgttgttg aaggattaat gacaactatc cacgcttaca caggtgacca aatgactcta
     6001 gacggacctc atcctaaagg tgacttccgc cgtgcacgcg ctgctgctgc aaacatcgtt
     6061 cctaactcaa caggtgctgc taaagctatc ggtttagtaa ttcctgaatt gaacggtaaa
     6121 ttagacggag ctgctcaacg tgttcctgta ccaacaggtt cattaacaga attagtaaca
     6181 gttcttgaaa aagaagtaac tgttgacgaa atcaatgcag caatgaaaga agcttcaaac
     6241 gaatcttacg gatacaacac agacgaaatc gtttcttcag atatcgttgg tatgacttac
     6301 ggttcattat ttgatgctac acaaactaaa gtaatgacag ttggcgacaa acaattagtt
     6361 aaaactgttg cttggtacga caacgaaatg tcatacactg cacaattagt acgtacttta
     6421 gaatacttcg ctaacttata agatagacaa acgtacaagt gtaaattata gacatgtaca
     6481 tttaaaagcg gggaagcaat cgcgcttctt ccgctttttt tgataataag tttattcagg
     6541 aggaaataaa atggctaaga aaacagtgaa agatatcgat ttaaaagaca aaaaagttct
     6601 tgtccgtgtt gacttcaacg ttcctttgaa agacggcgtg atcactgacg acactcgtat
     6661 caaagcagca ttgccaacaa tcaactacgt tctagaacaa ggcggaaaag caatcctttt
     6721 ctctcacctt ggacgtgtaa aaacagaaga agataaagaa ggaaaatctt tagctccagt
     6781 tgcaaaacgt ctaggcgaat tattaggtaa agaagtaaca ttcgtacctg aaactcgcgg
     6841 agaacaatta gaagaagcaa tccgcaacat gaatgacggc gacgttgtcg tattcgaaaa
     6901 tacacgattt gaagatattg acggtaaaaa agaaagcaaa aatgatacag aactaggtaa
     6961 atactgggct tcattaggcg atgtatttgt taacgatgct ttcggtacag ctcaccgtgc
     7021 acatgcttct aacgtaggta tcgcttcaac tggtatccca acagttgctg gattcttgat
     7081 ggaaaaagaa atcaagttca tcggtgaagc agtagaagaa ccaaaacgac caatgattgc
     7141 aatccttggt ggcgcaaaag tttctgataa aatcggcgta atcgaaaact tgattcctaa
     7201 agcagataaa atcttgatcg gtggcggaat gacttacaca ttctacgaag caaaaggaat
     7261 caagatcggt aactctctag tagaagctga taaagtggaa ctagcaaaag aattaatcga
     7321 aaaagcaggc gacaaacttg tcttgccaat cgacaatgtc tgtgcaccag aattttcaaa
     7381 tgatgttgaa acacaagtaa tcgaaggaga cattcctgat ggtttgatgg ctttagacat
     7441 cggcccagca tcagtaaaat tatttgctga tacattacaa ggtgcaaaaa cagttgtttg
     7501 gaacggacct atgggtgtat ttgaaatgag taactttgct aaaggtacaa tcggtgtttg
     7561 tgaagcaatc gctaacttag aaggcgcaac aacaatcatc ggtggcggag actcagctgc
     7621 agcagcagaa caattaggat ttgctgacaa attcacacac atctcaacag gtggtggcgc
     7681 aagcttggaa ttgcttgaag gtaaagaatt accaggacta gcagcaatca acgataaata
     7741 aacgaccaag tttgctcaat tctgacgagt aatcgatcaa aaggtgaagg gagttttcat
     7801 gactccctag ccatttttca ataaattaag gagtgtgttc ctcaatgcgt aaacctatta
     7861 tcgcaggtaa ctggaaaatg aacaaaactg catcagaagc aaaagaattt gctgaagcag
     7921 taaaaaacaa aatcccttca aatgacaaag ttgattcagt catcggatct cctgcattat
     7981 tcttacaaga attaatgaca tcagctgaag gtacagaact aaaaatctca gcacaaaact
     8041 gctactggga aaactcaggt gcgttcactg gtgaaacttc accagcagct cttgctgatc
     8101 taggtgttca ttatgtgatc atcggacatt cagaacgtcg tgaatatttc catgaaacag
     8161 acgaagacat caataaaaaa gcaaaagcta tctttgcaaa tggtatgaca ccaatcctat
     8221 gctgcggcga atctttagaa acctatgaag caggcaaaac tgctgaatgg atcgaaggac
     8281 aaattacagc tggtttagct gatctttcag ctgaacaagt gtcaaacatg gttattgctt
     8341 atgaaccaat ctgggcaatc ggtactggta aatcagctga cgcacaaatc gctgacgaaa
     8401 tttgtggagt tgtacgtcaa acagttgaaa aactttatgg aaaagaagta tcagaagcag
     8461 ttcgtatcca atacggcggt tctgtaaaac cagaaaatat tgctgaatac atggcaaaag
     8521 aaaatgttga cggtgcttta gtcggtggcg caagcttgca agcagattca tttttgtcat
     8581 tgttagacgc tgtaaaataa ttgttgaaaa tttgcgaaaa atgcaagaaa tgtttgcact
     8641 aatgagcaaa attaactaaa atcgtatatg agggaaatcc cttaggaaaa aacaaactca
     8701 aaggagagac aaaacatgtc aattattact gatgtttacg ctcgcgagat cttagactca
     8761 cgtggtaacc caacaatcga agtagaagta tacacagaaa gcggagcttt tggccgcgga
     8821 atggttcctt caggtgcttc aactggtgaa tacgaagcag ttgaattacg tgacggagac
     8881 aaagctcgtt atggcggtaa aggtgtaact aaagctgtcg acaacgtaaa caacatcatt
     8941 gctgaagcaa tcattggcta cgatgttcgt gaccaaatgg ctatcgacaa agctatgatc
     9001 gctttagacg gaactcctaa caaaggtaaa ttaggtgcga acgctatttt aggtgtttct
     9061 atcgctgttg cacgtgcagc tgctgactac ctagaagtac ctttatacca ctacctaggc
     9121 ggattcaaca caaaagtatt gccaacacca atgatgaaca tcatcaacgg cggatcacat
     9181 gctgataact caatcgactt ccaagaattc atgatcatgc ctgttggcgc tccaacattc
     9241 aaagaagctc tacgtatggg tgctgaagta ttccacgcat tagcttcaat cttgaaagct
     9301 cgcggattag caacttctgt tggtgacgaa ggtggatttg ctccaaacct tggttcaaac
     9361 gaagaaggtt ttgaagtaat catcgaagca atcgaaaaag ctggctatgt acctggtaaa
     9421 gacgttgttc ttgctatgga tgctgcttct tcagaattct acgacaaaga aaaaggtgtt
     9481 tacgtactag ctgactcagg cgaaggcgaa aaaacaactg acgaaatgat caaattctac
     9541 gaagaattag tttctaaata tccaatcatc tcaatcgaag atggattaga cgaaaacgac
     9601 tgggacggat tcaagaaatt aactgacgta ttaggcgaca aagttcaatt agttggtgac
     9661 gacttgttcg taacaaacac tcaaaaatta tctgaaggta tcgaaaaagg aattgctaac
     9721 tcaatcctta tcaaagtgaa ccaaatcggt acattaacag aaactttcga agctatcgaa
     9781 atggctaaag aagctggcta cacagcagtt gtatctcacc gttctggtga aacagaagat
     9841 tcaacaatct ctgatatcgc tgttgcaaca aatgctggcc aaatcaaaac tggttctcta
     9901 tcacgtactg accgtattgc taaatacaac caattactac gtatcgaaga ccaacttggt
     9961 gaagttgcag aatacaaagg tttgaaatct ttctacaact taaaaaacaa ataattcttt
    10021 tctaatgaag atacatgatc gactattgaa agatagtcga gtatgatcac ttagttaagt
    10081 aatcgaactc tctacttgct gaaatatcag caggtagaga
//
LOCUS       DACDEG010000089         9948 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.093, whole genome shotgun sequence.
ACCESSION   DACDEG010000089 DACDEG010000000
VERSION     DACDEG010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.093"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            131..3358
                     /gene="fss3"
                     /locus_tag="IV142_002261"
     CDS             131..3358
                     /gene="fss3"
                     /locus_tag="IV142_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP8485868.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            3471..3785
                     /locus_tag="IV142_002262"
     CDS             3471..3785
                     /locus_tag="IV142_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8485869.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3798..4172
                     /locus_tag="IV142_002263"
     CDS             3798..4172
                     /locus_tag="IV142_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8485870.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            4182..4517
                     /locus_tag="IV142_002264"
     CDS             4182..4517
                     /locus_tag="IV142_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP8485871.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            4600..5940
                     /locus_tag="IV142_002265"
     CDS             4600..5940
                     /locus_tag="IV142_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP8485872.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            6018..6692
                     /locus_tag="IV142_002266"
     CDS             6018..6692
                     /locus_tag="IV142_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8485873.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            6925..7359
                     /gene="dcm"
                     /locus_tag="IV142_002267"
     CDS             6925..7359
                     /gene="dcm"
                     /locus_tag="IV142_002267"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP8485874.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            7360..8067
                     /locus_tag="IV142_002268"
     CDS             7360..8067
                     /locus_tag="IV142_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP8485875.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            8057..8347
                     /locus_tag="IV142_002269"
     CDS             8057..8347
                     /locus_tag="IV142_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485876.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            8606..9790
                     /locus_tag="IV142_002270"
     CDS             8606..9790
                     /locus_tag="IV142_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8485877.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            9805..9924
                     /locus_tag="IV142_002271"
     CDS             9805..9924
                     /locus_tag="IV142_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP8485878.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT         3295 a         1679 c         2246 g         2728 t
ORIGIN      
        1 aaatttttag gctcattcat tctcaaatcc attctattca gtcaataaaa attttaaaaa
       61 gactttataa acaaagatgt tcaagcgagc atcttatttt tttgtgctta atttgaaagg
      121 agaacatttc atgtataaaa aacaaaaaaa gaaattatgt gtattaacta ttttgagttt
      181 ggctgttcaa gcaattgttg gagcaattgc tcctacgatt acttttgctg atgaaattac
      241 ccatccacaa acggtaaccg tagaactaga tttagcacat caatatgctg ttgaaggaac
      301 ttttagtgat ggtcgtccaa tgtcagaagt cactgttccg cattatgcag tgtacaacgg
      361 ctttaaacaa gatattttct gtattgaacc aggagtcccg atttacaatg agttcactcc
      421 tggctatgag aaaaacccgt taccagatat gtccgataaa gccaaattag tttccgtttt
      481 atggaaaaaa gtaggtacag atgcagatac tcatattgtg gcacagaaga tgatctggca
      541 agaagttaat gggtataccc ttcactcaat aaaacgatca gatggtagtg ccgtaaatat
      601 cgcagccatt gaggcaaaaa taaatcaagc cattgacgat tatcagaaga aaccaacctt
      661 ccataatagt acggctaaaa cagtattagg gcaatcgact actgtgacgg atacgaataa
      721 tctgaatttg tcagagttcg atgaggttgt ggaaaatgcc gcaaacattg attatcgtgt
      781 gaatggtaat cagttgataa tcaccccaaa tgccaattca aaagaaagtg gtgtgctgac
      841 gcttaagaaa tctgctggta ccggaacacc tgtcgcctat aaaaaggctg gccaacagac
      901 tttaatggcc ggggcgattg ataaacctaa tacctacaca atcaaaattg atgtggaaac
      961 ggagggttct ttaaagatca aaaaagtcga taaagaatcg ggtgatgttg taccaggaac
     1021 ggttttccat ttagactttg gaaaaacttt acctgcaaaa gacgtgacta ctgacaaaga
     1081 aggcattgct acattggatg agattcctca tggtacaaaa gtaaccatta ccgaaaaatc
     1141 agtaccggcg ccttatacga ttgataccac ccccatgact accaccatta aagcaggcga
     1201 aaccatttac gtgacttcaa aaaacgcacg ggaaaaaggg caaatcattc tggataaaag
     1261 cggagttgaa acagggagtg atctttggaa tgacaattat tccttagctg gtaatacgtt
     1321 tgccattcgc aaagacagtc caaccggtga aatcgttcaa gaaatgacca cggatgaaaa
     1381 cggtcatgcg gaaacaccaa aagagattgc taatgcgttg gaattgggaa cctattatgt
     1441 gaccgaaact aaagctagtc atgggttcgt gaatacgttc aaacctgtaa aagtcgaatt
     1501 gaagtatgcc aatcaaaccg tagcccctgt taccagtaac gtaaaagggc aaaatcaaga
     1561 agtcactggc gaaaccactt tgacgaaaga agacaaagat actggggata aagcacaagg
     1621 taaggcagtc tttgagggtg ctgaatacac cttgtttact gctaaggatg gaaaagctgt
     1681 taaatggagt gaggctttta aaccagagtt agcgaaggga acgaaaggtt ctgatgaaac
     1741 ggtcactttg actttagacg aaaagaacca agttgccgtt aaacatttag ctatcaacga
     1801 gtattattgg caagaaacaa aagcgcccga aggatatagc cttgatgaaa caaagtatcc
     1861 tgtatccatc aaaaaggtgg atgatagcga aaaaaatgcc gtgattaccc gaaatgttac
     1921 ggcaaaagaa caagtgattc gctttggctt tgatttcttt aaatttgctg gatcagctgc
     1981 gggcactacc gaaactggct tcaatgatct gacctttaaa gtgtcgccat tggaagggac
     2041 caatgaaatc acaggtgccg aagatgaagc gaccacagct tataacgagc aattagggtt
     2101 tgatggttat ggcaagtttg aaaatcttcc ttatggggat tatttacttg aagaagtaga
     2161 agccccagaa ggttttcaaa agattacgcc attagaaatc cgttctactt ttaaggaaaa
     2221 caaagaagac tttgtgaaga gtgagtatgt ctttaccatt accgaacaag accaaaaaca
     2281 accaattaaa acggtgaccg ttccttacga gaagctgacg aacaaagcat tttctgttag
     2341 tttgaaccgt ttaatgctct atgatttgcc tgaggaagaa gatagtttaa cttctcttgc
     2401 gacttggaaa gacggaaaca aagaattaac ttctctggat tctaccgagc tagttgataa
     2461 attgagttac aatctacatg aaatcaaaga agactggtat gtggtagcgc aagccattga
     2521 tgtggatgcg acaaaagctg cccaagaaaa agatgaaaaa gccaaaccag tggtgattgc
     2581 agaaacaagc gcaacgttgg cgaacaaaga gaaaacagga acttggaaaa ttcagcataa
     2641 attaaccgcc gaacaagttt taaataaaac tattgtctta ttcaactatg tgtatgaaaa
     2701 taaggaagct tttgaagcag gcgataagcc agtagcgaag gatgttagct tgaataacca
     2761 agcccaaact gtcagttgta cagtggaaca ccatgtttct atccaaacaa aggcccacct
     2821 agaaaacggt tctcagacct ttactcatgg tgatgtggtg gatatgtttg atgatgtgtc
     2881 gattactcat gatgtactgg atggctcaaa agaagctttc gaaacaattc tgtatgcttt
     2941 attaccggat ggtacaaaca aagaaatttg gaaatctggc aagattgatt atgaagtaaa
     3001 tgataaagaa ttcaccaaaa ctgtgcttgc gaaaaaagta gataccggaa aatatccaga
     3061 aggaaccaag tttacttttg cggaaatcaa ttacgataaa gatgggaata tcaatggaaa
     3121 acacaatgaa gatttgaaag aaaaatctca aaccttaaca ccaaaagaag ttccaaccat
     3181 actaagtacg ccgaaacaac cggaaacacc aactgttcca agtggctctc aagaatctag
     3241 tcccacagtg aagaccttcc ctcaaaccgg ggagaaaaat tccaatgttt tactgttcat
     3301 tggttttact ttgatttttg cgacggcggg ctattatttc tggaatcgcc ggaactaagg
     3361 tgatgagatg atacaagtaa atcctaagaa ccaacgtgcg cgaccgcctc ctaaaaaagt
     3421 agtcgcgcta tttttgtatc caaaactaac aatgaaaaga ggaaaagaag atggaattga
     3481 aatttgtcgt gccagatatg gctgaaacct ttggaaagtt aagttatgcc ggtgaaggag
     3541 aaattataac agaaggatat ggacggaata ctacggtgat tggtcgcagt tatcatttgt
     3601 attccagtaa gcaacgggcc gatgatattg aggtggtagt agctgcggaa gccggtgaaa
     3661 aggattttga tcaagaccaa ccgctaaaag ccgtgaatcc ccatttggtt gccaaaggct
     3721 atgagattga gaatcgtggg tttactgatt acgtgttgta tgttgatgat ttagtgaaag
     3781 cataggagga aaagaagatg agattaacag aaggtattgt cgtagattca ggtttaacat
     3841 ttgggaagtt gcgtttttcc gcattacgcc gagaagtacg gaagcaaaat gaggatggaa
     3901 caattggtaa tgaggtaaaa gaacgaacct ataatttaaa atcctctgca caagggcgaa
     3961 tgattcaagt tagtatccca gctaatgtac ccttacgcga gtttgcttat gatgcagagg
     4021 tagagttggt caatccaatc gtggataccg ttgccaatta tgtttttcga gaaggaacta
     4081 ccgttaattg gttcattaag gcggatgatt tagtcttgaa gcggcagccg aatcaaggaa
     4141 atcctacgaa ccaaaatgaa agaaagaaat aggtgaagat tatggaaatt aatgttgaac
     4201 atattttaga ttgtctcgat caatatggaa aaggcgagct gactgaggaa caactcgtta
     4261 aagcattaac ctctgatgaa aaaatgtttc tgattataca tcaaggttta cttggagaaa
     4321 atagcgacag tgaagaagac tctgatgtac tagactggtt aggagaagaa gagtctttct
     4381 ttatagttgt cgaagtaaat gaaaacttat gtcggcaggc agaatccatt ttggaagaaa
     4441 ttggtgtgga gatgccggat gctattgaag gctttttaaa tcaattggtt gaaacgaaac
     4501 aactcccctc ctcgtagcag taaatgacta gcataacttt ttgaaattgc ctaatgaaat
     4561 cctattgggc tttttcgatt ggaggtggaa accatcaaaa tgtacaaagg acatcgcata
     4621 cgcgcaggag atcagcactt ggtttatcac tttgttcttg gctggctact cgctctgttc
     4681 atcggttgga tgagcgtctt ttattttcaa gagtttagac aatttgatat ttctaagcta
     4741 tcactttcta ctattgaaat cgtttggtct ataaaagatt tagtatgtct gcttggaagt
     4801 ctcgcttttt caggagctat gatattgctt tatattcact tttttcttga tcattggcga
     4861 agcttgtggc atcgacaaaa gctagctcgg atgattctag agaaccattg gtatgaagtg
     4921 aaacaaactc aaagtgaagg ctttttcaaa gatttgaata gtagccgaac cagagagact
     4981 atcagttact tccccaaaat ctattatcga atgaaagatg gattactgtc tattcgcgtt
     5041 caaatttcac ttgggaaata ccaagatcag cttttaaaat tggaaaagaa gttagaaagc
     5101 ggactgtatt gtgagttagt agaaaaggaa ctcaaagact cctatgtgga gtatacttta
     5161 ctgtatgata tgatcgccaa tcgaattggg atagacgaag tagtggcaga aaatgggact
     5221 ctgcgattga tgaaaaatca agtatgggct tatgattctt taccccatat gttaatcgct
     5281 ggtggcacgg gtggagggaa gacctatttc ctcctcacca ttatcgaagc actattgaag
     5341 tcagatgcag aattgtttat cctcgatccc aaaaatgcag atttagctga tttaggtacg
     5401 gtgatgcctc atgtttattc acaaaaggaa gaaatttctg cttgtgtgga agatttctat
     5461 gaacgtatga tggctcgtag caaggcaatg aaagaaatgt ctaactacaa aacaggagag
     5521 aattatgcct atcttggact tccacccaac tttttaatct ttgatgaata cgtggcttat
     5581 atggagatgt taacgacgaa agaaagtgcg gtgattttga ataagctcaa acaaatcgtg
     5641 atgttgggtc gtcaatctgg tttctttctg attttggctt gccaaaggcc ggatgcgaaa
     5701 tatttaggag acggaatccg cgatcaattc aactttcgcg tggctttggg tcgtatgagt
     5761 gaactcggat attccatgat gtttggggag gtcgaaaaaa acttctttat gaagcacatc
     5821 aaaggtcgag gttatgtgga tacaggtggc agtgtgatta gtgagtttta tacgccactt
     5881 gtaccaaaag ggtatgattt cttagattca attagtaaat tagaaaagat gaatgattga
     5941 gtgatcttca atactgactt tatacttcaa gtaacgtata atacttttat atagtaggaa
     6001 gaaaggggat aattaatatg gaaagtaatg attcttattt agagtatgca aaagcaaata
     6061 aggataagtt tatttcttat attatcaaag ggaaatcccc cgaagttgga cgagacgcag
     6121 tttttatggc agggagtcct ggggctggaa aatcagaggt tgccattgga ttagctgaga
     6181 attatgataa ccatgtgatt attgatgcgg attactttcg cacccagttt cctgattata
     6241 atggaaaaaa ctctagtctc tttcaaaaag catcttcttg gttggtagag caggcattta
     6301 gatatgtctt agatcatggc tattctttta ttctggatgc aacttttgca atattaagtg
     6361 ctgaaaaaaa tatctcgcgg gcgttgaaaa aacattttcg agtgacgatt ttctatgttt
     6421 atcaagaccc taaaatcgct tggaaattta cacaagaacg tgaaattgca gagggacgtc
     6481 atgtgccgaa aaaggcattt attaacgctt ttttcaaatc aagagagaac attgcaaaag
     6541 taaagagtaa acatccagat gtattgcttc atatcataat aaaagattat acgaataata
     6601 tttccgaagt tcattatgct acggataatc tagaattatt tttaccacta gggtatagta
     6661 ggaaagattt ggaggttcaa ttacatgact gagaaagaac aagtaacgaa aattgtaaag
     6721 aaatacaata agagcattgc ggatttatcg gaaaatgcta ctgcaaaaga gttcaaaacg
     6781 gtgattaaat atgtagccga tcaggccaat gaaaaacaac ggaaattagt tggattaaat
     6841 aaaaaatagt aggacaaatt aataatgtat gagccaagag caaaatgttc ttggcttttt
     6901 tctttgcaat gaatgaggta atgtatgaga tttttagatt tattcgccgg tattggtggc
     6961 tttcgtttag gtatggaaca ggcgagccat cattgtatcg gtttttgtga aattgataaa
     7021 tttgctagac gtagttataa agcaattcat gatacaagta aggaggtgga aatgcatgac
     7081 atcacaagtg tatcagatga gtttattcaa tccctcggac cagtggacat cctttgcggc
     7141 ggatttccgt gccaagcttt ttcaattgcg ggaaagcggc aaagattttc cgatactcga
     7201 ggtactttat tctttgaaat cgctcggttc accactcttc tccgacctaa gcttttattc
     7261 cttgagaacg tccgaggatt gctcaatcac gaaggagggg ctacgttcga gacgatcctc
     7321 cgaacgctgg atggattggg gtatgacgtg gagtggtagg tgcttaactc aaaggcctac
     7381 attccccaaa gccgggagcg gatattcctt atcggacatt ctcgagacgc atgtaccgaa
     7441 caagtatttc ctatcattgg accgtcttca acatctgatc aaaacatcag aaatttggta
     7501 aatatcaatc cttccaatcg aggaatgggt ggacaagtct atggttcaga tggcgtagcg
     7561 ccaaccttaa caggtgatga aggaatcaaa atcgcactgc cagcaaggga tggaatctct
     7621 gtagcaggac tattaccggg aaagtttgaa caaggtaatc gagtttatga agttactgga
     7681 accgccccca ccttgtcaac gaaacaaggt ggtacaaaaa tcatgattcg aaaaaatgaa
     7741 acagaatatc aagaggtgaa gccagaagac agtgtgaatc tggcttttcc taattccaca
     7801 agtcgccgag gtcgtctggg aaaacagagt gttcatacct tactaacagg agaccaacaa
     7861 gcgatcgtca cggatcagta tcaaattcgg aagctaacac ctagagagtg ttggcgactt
     7921 cagggctttc ccgattgggc ttttgatcga gattctcaag tcaattcaga tagtcagttg
     7981 tataagcaag ccggaaactc tgtaacagtt ccggtcattt ttgatattgc cagaagatta
     8041 aaagagttgg atgtacatgg tttatgatga gttgattgga gaaatttatt gggtgattgg
     8101 aaagattcag tcagacccag aattagaaga acaattgcga cggttaaaat ttgatattcg
     8161 caagaatggt gtcaaagtcg ctggcgatcc atatttaatg aatgaggaga cggatgctcg
     8221 aattgaaata aatcaagtaa ttgcagaatt tgagcggatt gcggaccaag caaaagaacc
     8281 agatattcgt caatacttgt tagaaatgaa agcagagttg gaagtaaatg gtattacaga
     8341 tgagtaacct aaaccaacac aactgactgt taggcgctga ccgcggagga ttcgcgaagc
     8401 agaaatcctc cgtggtgctg tgcctgttca tggacggcgt tagccgacca tgatgtctca
     8461 cccccgtatc taacaggggg gtacaaaaac acaaaaaata agtaagcaac cattaagaat
     8521 gcttgttgta tcaaagatag taagtaattt gtataggtgt taactcattt gaaaaagtta
     8581 acaccttgaa tttggaggtg agaacttggc acaacggaat ttagattatc ggctattaaa
     8641 agaccgacgc aacgaatacg gcgtttcaca aaataaattg gctacggctt gtggacttag
     8701 tcggccgtac ttaaatcaga tagaaaatgg tggcgtaacc gcatctataa aaaccatgag
     8761 gaaaattttt gaccaactgg aaagctttaa tcccgatttg cctttgacgt tactgtttga
     8821 ctatgtaagg attcgctttc ccacaacgga tgcacgaaaa attattcaag aaattctcca
     8881 tttgaaattt gagtatatgc tccatgaaga ttatgccttt tactcctatc aagaacagta
     8941 tgtcatgggt gatattgtgg tgatgttgtc tcatgaagaa gataaaggtg tgcttttaga
     9001 attaaagggg cgaggttgcc gacaatttga aactttttta ctcgctcaaa agcgtagctg
     9061 gtacgacttt ttcgaggatt gtctaaaggc cggtggcgtg atgaaacgct tggacttggc
     9121 aatcaatgat cgagtggggc tattggatat acctgagcta acgaaaaaat gtcagaagga
     9181 agaatgtatc tccttgtttc gtacctttaa aagttatcgt tccggggaac ttttaaaggc
     9241 tgatgaaaag gacggcatgg gcaatacctt atatatcggc agtctcaaga gtgaggttta
     9301 tttctgtttg tatgaaaaag actatgaaca atatatcaag ttagggattc cattagacca
     9361 aactgagacg aaaaatcgtt ttgagattcg actgaaaaat gaccgtgctt ttcatgcgat
     9421 tcaagatttg ttaaaaggtc gtagcattga gagcaccacg ttttccatta tcaatcgtta
     9481 cttgcgattt gcggataaag tagaaggtaa aaggcgaacc aactggccct tgaatgaaca
     9541 atgggggcgt tttattggac ggaatcggaa agagattcaa ttaacttccg agccaaaacc
     9601 gtataccatt gaacgaacct tgaattggct aggccgacaa gtcgccccta cgtggaaaat
     9661 ggctaaggag ttggatcgat tgaatcggac aacctatatc caggatatgg tacagaatgc
     9721 acgcttatca gaccgacata aaaagatttt ggagcaacaa agtatggcaa ttgaaaattt
     9781 gattatatga aaagaggaat cgtcatggga attgtaaaag atattttttt ggtatttggt
     9841 ggcagtatgc ttggtgttac tgtgatgtgc ttgatgaatg caagtgcagc agcggatcga
     9901 gcaatggaaa agaaaggaaa gtgacaagat gaatttcgga caaaattt
//
LOCUS       DACDEG010000090         9862 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.094, whole genome shotgun sequence.
ACCESSION   DACDEG010000090 DACDEG010000000
VERSION     DACDEG010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.094"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            205..684
                     /locus_tag="IV142_002272"
     CDS             205..684
                     /locus_tag="IV142_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485879.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            717..2945
                     /locus_tag="IV142_002273"
     CDS             717..2945
                     /locus_tag="IV142_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP8485880.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3074..4717
                     /locus_tag="IV142_002274"
     CDS             3074..4717
                     /locus_tag="IV142_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP8485881.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4790..5335)
                     /locus_tag="IV142_002275"
     CDS             complement(4790..5335)
                     /locus_tag="IV142_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP8485882.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5358..5843)
                     /locus_tag="IV142_002276"
     CDS             complement(5358..5843)
                     /locus_tag="IV142_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8485883.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5833..6645)
                     /locus_tag="IV142_002277"
     CDS             complement(5833..6645)
                     /locus_tag="IV142_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP8485884.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6784..8316)
                     /locus_tag="IV142_002278"
     CDS             complement(6784..8316)
                     /locus_tag="IV142_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP8485885.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8425..9456)
                     /locus_tag="IV142_002279"
     CDS             complement(8425..9456)
                     /locus_tag="IV142_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8485886.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3282 a         1832 c         1897 g         2851 t
ORIGIN      
        1 aaatattcca acatcatctt ggactttcta catgttacta atgagcgtgt aaaagaacga
       61 gatggtgttt ttatttctat caatgaagct ctttttcttt gcaatcctca cttcaatcct
      121 ctataatgtc gcatatagtt tagtaaatga gtaaactact aatttactta tttactaaaa
      181 ataaaaggat gaggagttaa aataatggaa ataccaacaa atttaaaaca taaaccagta
      241 atcgttgtag aagactatga taaagttgat ggaagaaatg cattaaatat ggatgcaaaa
      301 gggctttcac tgggccttgc tcagtggaat gatcgtggaa aagtcgatat ctcaggcaag
      361 gtatggcgac atactggaga aaaatggtca cgacagtcgg aagaattacc aatcacacgt
      421 attttagatt tagcgatctt agctgcgcaa ggaagtttat acttccaaga tgcttatcgt
      481 tatgaaaaat tttatgatcc agaaaatcca gtcgtagaca tcattggatt acaagggaac
      541 cgaatgacgg tagaagtaga taccaaaaat ccaaagatcg atgaagacat cattttgtat
      601 tacgatacac tacaaaaaga cggagaactg attggggaac gattccgtat tttaaaacgt
      661 cttttagatg agctaggata ttaatagaca gagatgatca aaaggggaag agaagcatgg
      721 agaatttctc actaacagaa aaccgaacct tttcacggct agaaaagcaa atgatctgga
      781 agaaaccgaa aagccatcgg acaagtgcag aagaatggcg gatcgcctcc gaaatcaaag
      841 ggaattggca agatcctgaa atgaaaatca gtaacgtact tttggaagga gatgccgggt
      901 cggggaagac acaattagcc aaagcactat ctgctgatct gcaacttccc tatacaaaag
      961 tgacctgttt tgcagatatg gacaaatcag atgttttcgg agccttatta cctgtagtag
     1021 attctaataa ggaacaagac caagaattac tagaagctat ttaccaaaca gatacacttg
     1081 aagctgtact gacactgatc cagaaccatt acggcattga tcctttttct gctaaagaaa
     1141 aattagcaga gctagttcaa aggatcgaag ctgattatcc agatgcaatc cattacagct
     1201 attacccttc tgaaatcgtt cgagcgattg aaaaaggcta tttattagag attcaagagc
     1261 cgactgtcat ccgagatgcc tctgttcttg tggcacttaa ctcagcgctc gaaccaaatg
     1321 ggatgttgaa tttaccgaca ggaatcattc gtcgccatcc agattgcgtc attgtgatca
     1381 ctacaaatcg aaattaccaa ggaaatcgcc cattaaatga atctttaaga gatcggatgc
     1441 aacatgcgga aaaaatggat cttcctgcct tagaagtcat ggcagaacgt gcgatggcga
     1501 aaactatgat ccaaaaacaa gaacttctct taaagatggc agagattatc cgtttgttgg
     1561 atgaaactgc aaaagctaac gccatcaaag gtgtagcagg aatgcgttct tatttttatt
     1621 gggcaaatac ctataagcaa ggacaagatt tactccagtc gatttatcca aaagttttgt
     1681 ataaattatc cactgattca gatgaattgg caattcttga acaagctttg tctgacagtg
     1741 gattgctact ggaacttcag gacatattga gaaaagaaaa atggggtcat cctcaatctg
     1801 agcatacaaa agaaagaacc attagcgccg aagaagcaaa tgaacgaaac atcactgaaa
     1861 atgctgaaga aattctgcgc ttagatgaaa agaaatcgtt gtctgctgaa gaaccaaatg
     1921 agggagaaga gaaacaagaa gaaacaatag aaaaaaacga gattcctcct caaatgcaaa
     1981 aagaacgcag tagcgatatc gaaggaaaaa atcaaaaaga acaacaaaca ggagatgatt
     2041 ctcaagaaat gtctgcttct gacatggaag cacatgataa ggaaatcaag aagcaactga
     2101 ataaagaagc gcgacaaatc atgaaggaaa cgattcatga aaaagaaggt ctcatcgttc
     2161 accgaccagc tttccaagga aaaacgccgg aagcacgtga gctacacatg caagtaatgc
     2221 caatagtaga atcacttagc cggcaaattt tagagttact ggataatgaa caaacagaga
     2281 cgtaccaaaa aggaaaatac gaaggtcagc ggttcaatgc aagcaaagta gcatatggtg
     2341 atttgagaaa ttttgacaaa aagaacccgc cacatgagca accttcttta gcggtagctg
     2401 tacggatcga tgaatctggg tcaatgatac gggatgatcg gatcgaagcg gcaaaaaaag
     2461 cagcgattgc cattgccgaa ttttctaaga aagtcgatat cccattgttg atttacggag
     2521 atacagcaga ccgttcgtca agagaaaaga cttctttgtt ttcttataaa gaatttgaag
     2581 acggatttca atggttaaat gaaaaattag tcactatgaa accaaggcaa aacaatcgtg
     2641 atggtgctgc acttcgttta gcagcagaaa aattaaacag acaatccgca acaaccaaac
     2701 tgttgcttaa tattagtgac ggtcagccaa aagcattgcc ggattataca ggcaaaaaag
     2761 caaaagaaga tatccagtct gttttgaaag aatatgaacg tcaaggaatc ctatttgtct
     2821 ccgcagctat tggccaagac aaagaagaaa tcaagtcgat ctacggtgaa agtcgttttg
     2881 tagatattac tgacttgaat gaatttccca agcaaatgat tcaactgatt gcccgctatc
     2941 tatgataaaa taaagctcaa cgaacgcact tagcagattc gttgagcttt tcgtttctag
     3001 ttaccggtaa caaggatact tctaaaatag aatcggttat aatcaagagg gaccgttaaa
     3061 ggagtgaaac gatatgatac aagaacaaaa atggtggaaa gaagcagtag gctatcaaat
     3121 ctatcctgct agtttcaaag atagcaatgg cgatggtcaa ggagacatca atgggatccg
     3181 tgaaaaactt aattatttga aagatcttgg tatcggcttt atctggatca atccgatcta
     3241 tcaatctcca tttgtcgata atggttatga cgtaagtgac tatcaagata tagaagagag
     3301 attcggaacg atggaagatt ttgatttatt gctaaaagaa gcacatgaat tagggatcaa
     3361 agtgattatg gatcttgtca tcaaccatag ttcggatcag catcaatggt ttgaagaatc
     3421 gaaaaaatct aaagaaagcc cgtatcgcga ttattacatt tgggtcgacg gggtagatgg
     3481 caaagaaccg aataattgga catcgatttt cggcggttct gcttgggagt attctcatga
     3541 aactggacag tattatctgc atgtttttgc aaaagaacaa ccagacttaa attgggaaag
     3601 cgagaaattg aaagaagaac tattcaatat gatccgatgg tggttggata aagggataga
     3661 tggatttcgt ttagatgcga tttctcatgt aaaaaaagat gaatattcag taaaagccac
     3721 agagaaccct ttctcaccct ttcaaaatgt ctcaggaatc gaagaacatt tgaccgattt
     3781 gaaacatgtt tttgaagagt acgatatcat gactgtcgga gaagcaagtg gtgtcactgc
     3841 agaagaagga ccacaatggg tagggaaaga cggttatttc gatatgatat tcgaatttga
     3901 tcatatccat atctggcagc aagaaaaaga agggcaatta gatgtcctta aattgaaaca
     3961 tgcattaagc gcttggcaaa cttcattgga tggtatcgga tggaacgctt tatatatgga
     4021 aaaccatgat gttcctcgtg ccgtttcagt gtttggagat actcgtcccg atttttgggc
     4081 tatgtctgca aaagcaatcg ctatgatgta tttctttttg caaggtacgc catttattta
     4141 tcaaggacag gaaatcggaa tgacgaatat gccctttgaa tcgatcgatc aagttgatgc
     4201 agtagattcg aaaagacttt ataaaaggct gcttgcggaa ggtaagacaa gagaagaagc
     4261 attggatatc attcgtgaaa caacgagaga caacagcagg acacccatgc aatggacttc
     4321 ggaacaatat gctggttttt ctacacatga accttggctg atcactaatc caaataccaa
     4381 aacgatcaat gtagaacaac aagaatatga accagagtcg gtcctgcaat tctataagaa
     4441 catgatccgc attagacaaa ccaataaagg tctgatatac ggttcttata aagaatatct
     4501 tcatgaacat ccacaacttt atgtctatga aagatattta gaagatgaac aatatcttat
     4561 tatggtaaac ttaacagaat cacttgctga ctatgaactt ccaaaagaag cagatcagtc
     4621 ctggacatta ctcttgtcaa attcctctag cggagaattt gaagctaaag gcatactagc
     4681 accttatgaa gctcggttat ataaaacaaa caagtaaaac ttcctcgtta aagttcatta
     4741 gttggacttt gcgaggaagt ttttcagatt gctctgtcaa ttactcattt caataattcg
     4801 ctctaggacc accgatatat ttaggacgag agcgaagggc tgtcacgtga gcttgtccaa
     4861 gggttttcaa acgaggtctg acaggtactt ggacatgctt gatatgcata ccgattgatg
     4921 tgtcaccgat atcgataccg gcttgtccaa ctacgtgttc cacttcgaca ggatcattga
     4981 actggtcaaa agcagcaaca gaacaagcac ctccagcatg tagtgcagga acaacagaga
     5041 caatttcgaa attttttgct tctgccactg ttcgttctac tactaatgct cggttcaaat
     5101 gttcacaacc ttggacagct agcgcaattt ctttaggatc caacaactct tttaacgtac
     5161 ggatgatcca ttgaccaact tccgcactgg aattttttcc aatatgtcct ccaacgactt
     5221 cgcttgtgct gcatcctaaa acaaaaagat cacctttttt caagtgtgcc tcagccaata
     5281 tgtcattaac tatttcagtc aattggtctt ttaattcttt ttctgacaac ttcattttct
     5341 aaaaccttct ttttcaatta atattgcttc cgtgcttgaa tacgttgaag acttgcagtt
     5401 ataggaaagg caagtcccat tccaatcaga ttttggaaga tgtttcctgg tatagaagcc
     5461 actccagccg gccaatcata taaaatccaa ccggcgatac catagccgat gatcataact
     5521 aagcttccta ataaaagccc taatccccac atacgagtag aagatttgct gaaagaacca
     5581 gcaacatatc cttgtaaccc gtggatcact aatgaaaaaa ctgcccactg cggatatcct
     5641 gaaatcaaat cgatcaatag accactggaa gcaccaacaa ctaatccacc cgttggacca
     5701 taaagaaaag cagcggtata gatcccggcc tcgcataatg tcacaaatcc attcgtagcc
     5761 ggtacaggga tcaaaattgt taatgaaagg gcaactgtca acgcaatcag catagcatag
     5821 atcgttaatt gcttagtttt catttctcat tcctcctttt tgaataccgc cagaccagac
     5881 acttccggta tgatcgttca tcagcacgcc attagaaata caatgggcaa caaatttttt
     5941 actttctgct actgctttgg taaaatctgc ccctaacaca agattcgctg ttattgcaga
     6001 agaaaaggca caaccggccc cgtttactgt tgtagtgtcg atttttttat gagaaaatac
     6061 ctcataatga tcaattgaat agagaagatc cacagcttca tctccagcca tccttgtgcc
     6121 gcctttgatc acaatgtttt ttgttcctga agcagtgagt tctttagcga gatggatcat
     6181 ttctgttaac gaagagggaa gagcttgacc agaaagtaaa gcagcttctg ccaaattagg
     6241 cgtaatcatc gtaacaagtg gaaaaagttc ttcgatgagc tgcgaaatat acaattgatt
     6301 gatcacagct tcagtttctt tgaatgccaa aacagggtct aaaatgattg ggatattttg
     6361 ttttgttttt atgaagtctc gaacaaccat gattgcttca cttgaattca ataacccaat
     6421 tttgatacca tctaatgaag tcattttatc aatcgaaact aactgtcgtt taagcaattc
     6481 caaagatata gattccagca taaatcgctc attaatatct agtgccccta tagaagtcaa
     6541 ggcgcttagt ccgaatagac caaaattctc aaaagttttt aaatctgttt ggattcctcc
     6601 accagcaaat ggatcagaac cgccaattgt caagattctt ttcataacat tttcctccat
     6661 gaataagagt ataacgaaat tttcaaaaaa gaaaaacagc caattggcat atttttatct
     6721 catccaattt aaaaaaacaa aaagaaaaga aagaccaaaa tggatctttc tttccttaaa
     6781 tagttaggac ttttgtgatt ttaattcttt ctcgatttga agccgttgca ctttcattgt
     6841 agctgttcgc gggattgcat caaaatcacg gatgataggg acgttcaagc gaggaagttc
     6901 atggacttgc tcccaccatc gattccaatc catttcttta tcaggagcta aagcaataat
     6961 cggttgtgga gaatgatttt tatcccttac aatcactact tctgccaaaa aatctaacga
     7021 atctaataaa aagtcttcaa tagctaatgt actattgatc gtttcgatca aatccacttg
     7081 ccgatctttc aaaaataggt ggccatgctt atccataaaa ccgtaatctc cgctatccca
     7141 ccaatcgcca taaacatttt cctggaaccg tgcatcttcc ttgtaataag tcaaagcccg
     7201 tcctttagac agtaattgta tatgaccatc tgtgttttca ggtaggacat tcccaactga
     7261 atctgtaatc cgagcgcttg tcatgtcttc cagtccaacg cccatatctc ttgcatcact
     7321 tgttttcaaa ctctctagtg tatgagcctt aagaatcatt ggtccgcatt cactttggcc
     7381 atatacttga agaaataccg catcctgtgc catcgacgtt cgcaaaaatg tcttcattgt
     7441 ctggttgttt atagcatcaa aagtggagtg atagtatcgg atacccgaaa aagcttctgg
     7501 atgctcctta gcagtaaaac gccactgaac aaagttattc ggatgggtct ctagtgcaat
     7561 tggccggtga gcttccaaca tatgaacaac ttttgaactt tgtgcgtttg ctaaaggcat
     7621 cataggaaat cccatggaca tcaaagaaga gataccaata ttgaatctgg aatgcactgg
     7681 agagatgtga aaagcgatta atttcttttc cgaaattttt gtgaaaactg ttttttgcca
     7741 tttcgtccgc catcccatag aatgagcaga atgacaaata agtttaggga taccagttgt
     7801 accagaagta tgggtcatat aagaaatttc atttttgtct aaaaatgatt gagaaacaga
     7861 caaagcaggt tgttccatta aataaagaat aggaatctgt tttgattttg ttccatttga
     7921 aatttctctc actcgctttt ttgtctcatc atcaaataaa atgaaagggt cttctaaacg
     7981 atcgataaat acttccattg ttgttgttgg tagatgataa gaagtcatga caggcactgc
     8041 accaaggtaa gacacagaaa cagctagaag gtatgtgtca aaagcggaac tcttataaat
     8101 aataactttg tctccggatt tcactccaag atgagccaat tgataagccc gtttcaacac
     8161 actttcgcag cattttttgt aagttgtttc aagtcctaat tctgggaaag ctgaaagtgg
     8221 ctcatcaaag ataatcgggg tctcggaaaa gttagctgca gcttcttgaa aattcgtaaa
     8281 taagttcaaa ggggaatacg ttatggtctc catcattcac actccattat cttttgttaa
     8341 gtaatataca aattcaagat aatcgtaaca taatccttta tataataaca tagtttattg
     8401 tgaaaaaaag atcatacatt tgttttatat ggaagaaacc cgaacgaatg ccgctaattc
     8461 gtgataagaa aaatcaacca tcttttccca gatatcttga aaagaatggg atgagaggta
     8521 tcgattacca cataaataat ttaccgcgtc agcatcatct aaatatagtt ctgcataata
     8581 acgatatgtt tttctgcata gttctggcga tacgttttta tatactgtaa ttttgctcaa
     8641 aatttggcga atagtccgag gagaaatttg ctgatcatgt gaggaacgaa aaacaaaaga
     8701 attttcgtcc gtattttgtg ctgttaactt aaaatagttc tccagaaatg gcatcgcttc
     8761 ttttggtatt ggtactttat tcagtctgtt ttcttttcta ttcagattaa gaatctgctt
     8821 ggtcaaagat acatctttac gtttaagttc tactacttca tggatcttta atccgcaata
     8881 cagaagaatg gcagtaattg caaggttcct cggtctattc ttttcttcaa agctagcttc
     8941 tctgcttgtt tgaaagcctt caatcgggat aaacaaatgc tcaaaatctt tgaaaagttc
     9001 tgcatcgata tatcgcggag ctgctttggt tgtttccggt aaacttgtac ccaaaagaga
     9061 aatctcttct tcaatcagtc ctttttgata caaaaatcgt aaaaaatgat ttaatgaata
     9121 ttgcttcctt gaaatcccgt aatagctatt ttcaaacaat tgctccgttt cttggttcgc
     9181 tgtagtttgg aacgaacgag tatgtcgata gattttagct aagaaattct gataatcttg
     9241 tttttctaaa tggataatca gctgatcatc cacttcttca gaaaatactt ttctcaaata
     9301 atcagtaaac agatgaaaat cttttgcgta ttcatagcga ctgcctaatg aaagggaaga
     9361 acgattttct tctaaaaact gttgaaagac ggaagggagc aagtgtgtac gttgctttaa
     9421 cttttcttgc agttgttttc tagttttgct gtccattaca aattccctcc aatagtgttc
     9481 attttctata ttttaccgca tttcatcaaa tgaagataga cagtttgtct tgatcttagg
     9541 aagcaaattt ttaactaagt actagctaaa agatatgaag taagaagata catttttagt
     9601 tattttatta gtgcagtcat tcttttgatc ttttaattga tttatcctct ttttagctat
     9661 gtggttactt catgagcaat gaaatggatt tggttcgttc taatggcagg aggctctttc
     9721 ttttatttcc gaaaaattag aaaacatttt ttcagaatcg attggctctc catttattca
     9781 ataatggttt gtctaacgat attgttggga taattcttaa agaagtatta gaaatagttg
     9841 gtacttcatt actttacatg aa
//
LOCUS       DACDEG010000091         9667 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.095, whole genome shotgun sequence.
ACCESSION   DACDEG010000091 DACDEG010000000
VERSION     DACDEG010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.095"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..650)
                     /locus_tag="IV142_002280"
     CDS             complement(<1..650)
                     /locus_tag="IV142_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8485887.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIG"
     gene            975..1391
                     /locus_tag="IV142_002281"
     CDS             975..1391
                     /locus_tag="IV142_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8485888.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            1388..1858
                     /locus_tag="IV142_002282"
     CDS             1388..1858
                     /locus_tag="IV142_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8485889.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            1872..2645
                     /locus_tag="IV142_002283"
     CDS             1872..2645
                     /locus_tag="IV142_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8485890.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            2659..3471
                     /locus_tag="IV142_002284"
     CDS             2659..3471
                     /locus_tag="IV142_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8485891.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            3525..4490
                     /locus_tag="IV142_002285"
     CDS             3525..4490
                     /locus_tag="IV142_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8485892.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            4606..7329
                     /locus_tag="IV142_002286"
     CDS             4606..7329
                     /locus_tag="IV142_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8485893.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELYITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            7342..7974
                     /locus_tag="IV142_002287"
     CDS             7342..7974
                     /locus_tag="IV142_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8485894.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            8873..>9667
                     /locus_tag="IV142_002288"
     CDS             8873..>9667
                     /locus_tag="IV142_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8485895.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLYL"
BASE COUNT         3261 a         1476 c         1885 g         3045 t
ORIGIN      
        1 ccaatcatat ggaagccacc gattaggtat tttatgggtt tagtcggaaa tgtttcaaga
       61 gcaatttcca tcatatcgat aatgccatga tgactacagc cagtaaagac aatcagaccg
      121 tcttcttgct caattacgac caataattca tctgaaaaat catcataaac aaatttatca
      181 tccatctttt tgtataaatt cttggtggtc gagtttgctt tgcctagggg gatgatatgg
      241 aagctcggag caagttctgt tttcgttttt attgtatgta aattctcatg cattttaagg
      301 atttcatttt taggaatacc aacatacaga aacgtggtta aaatctttaa ataatggggt
      361 aaagttacac cttcagctgt ataaataggg gcgtttcctg attttaaata tcttaacccg
      421 ccggtgtggt cagaatgacc gtgtgaaata atcactgcct ccagatcatt caaagtttcc
      481 cccattttag tcgcattata gactaatttt ttattagcgc cagcatcgaa gagatatttc
      541 ttccctgagt agttaattaa gagagataac ccatgtccac ttttaaagtt caaatttatt
      601 cttgagtttt caaataaaac cttgatttcc actataacat ctcctttcac atgaacgtgt
      661 attcatatat tgtagcacat taagttctat tttttgttgt aattaccgaa aaaaatatta
      721 atggacgctt tttacgcttt atttcgtatg tggaagaaaa tttcttagga tgtggcgggg
      781 gattggggca aagctaaatt ccaattacag tgatcattta attagacacc tttaaatacc
      841 cacactttca tcaacactat aatttaggga aagaaaagtg ttggcatgat atagaaaaac
      901 ctttaattaa cagcgtttcc taaaattggc acgcatcttg cttatagaaa agcgtaagga
      961 taggaggaat gaaaatggca actataattc ttgccagcca tggggaattt gctcaaggat
     1021 taaagcaaac agcgacaatg attattggag atagtgtacc aattcatgcc ttgtcagcat
     1081 ttagagatga agatgaatct attttggtac aaataaaaaa acttttagcc acaatagata
     1141 tagaagaaac ctacatttta actgatattc taggtggtag tgtgaataat gatatgctca
     1201 ctattattaa agagtatgag ggattgcaat taattactgg aatgaactta cctttagtta
     1261 tttctattgc aacctataat ggaaaaattg gggaaagtga gctagaaaag cttattaaag
     1321 agagtcaaca atcagtgatt gattgtagaa aattattagt agaacaactt actggaggag
     1381 atgacctatg attaagttag tacgtattga ccatcgtttg ttgcacggac aggtagtatt
     1441 ttcttggagt aaatcattaa atatcaatcg tattattatt gccaataaag aggcagcgaa
     1501 tgatgaattc aaaaaaatgt ctttgaattt atctaaaccg actggcatta aattaacaat
     1561 tttttcagtt gaacagttac tagaaaaaat gcctaaaatc gaaacactta acgaaaatat
     1621 catgcttatt tttggtaata caaaagaaat tcgtcaattt tgtgaagcct atcctaaagt
     1681 acaagaaatc aactatggag ggataatcaa aaaagaaggt tccaagcaat ttagtaatgc
     1741 cattttcttg aatgaatctg agcaagaaga tgccaaaaaa ttaaaagctc ttggcattaa
     1801 acaatttatt caacaggtac caacttctaa aaaagaagat ttgaatacaa tgttataaag
     1861 gaggaatgaa aatgctcgtt cagtcaattg gtttagggtt aatcggtgtt ttttgtattt
     1921 tagactcaag gcttttagga agaatgaatt ttgaacgacc gttgattgtc agtacgcttg
     1981 ttggtttggt acttggagac atggaaaaag gattaattgt tggagcctct ttggaattga
     2041 tgtccttagg attagtaaat atcggagctg ctgcaccacc tgatatgaat atggcatcaa
     2101 ttattgctac agcctttgcc attttatcaa atgcgaatgc agaagcagca ttaacgattg
     2161 ccattccaat ttcagtgtta ggtcaaatac taggtatctt aatgcggacg attttgtcaa
     2221 atttgactca cgttgcggat cgctttatcg aacaagggaa atttagccaa gcaaggagaa
     2281 ttcatatact ttatggctcg ggattatatg ctctcatgta ttttattcct atttttctag
     2341 caatttattt tggaacgaat cttgtttctc agattgttga tgcgattcct gagtggctaa
     2401 caaatggctt aacggttgca agtaaaattt taccagcata tgggtttgcg ttgttattac
     2461 aaacgatgtt aacgaaaaaa atggtgccat ttttattact tggattttta attactgctt
     2521 attctggatt gggaatcatt ggtattgcag ccttcgcatg tattgttgcg tttgtcatgg
     2581 ttcaagttca aggtaaaaac tcaaatggta accaaaatga gccagatcca ttagatgact
     2641 tataaggagg tagattaagt gaaagagaaa gatcaaaatg catggaaata tcggcagttc
     2701 ttatggcgtt catgggcaat tcaagcctca tggaactatg aacgacaaat gaacatgggt
     2761 tttatgtacg gaattgcacc aattctagat aacatttaca agaatccaga agatgaagca
     2821 atgaagaaag aagcttacga gcggcatatg atgtactaca actgtacccc tcaaacaagt
     2881 gcatttgtgt tagggttaac tgcttctatg gaggagcaat actacgaaga ccaagaaaac
     2941 tttaatccgg aatcaatcag tgcagtgaag acttctctga tgggaccgct atctggtgtt
     3001 ggtgattctt tctttcaagg aacgattcgg gtattagcct ttggactagg aattaattta
     3061 gcccaacaag gtagtatttt aggtccaatt ctagcaatgt taatttcctt tgtaccttcc
     3121 tttttagtta cttactatgg tgggaaaatt ggctataaca tggggaataa atacttgtct
     3181 aaactgtatc aagaaggttt gatggataaa gtaatgtatg tttgctccat tgtggggttg
     3241 atggtcattg gttcgatgat tgccagcatg atcgggatta caacaccaat tcaaatgggt
     3301 aaagcttttg tcttacaaga tgtattagat agtattatgc cacaattttt accattagga
     3361 ttaaccttct tcatgtactg gttattgcag aaaaaggtca aaacaggatg gatgttggca
     3421 atctgtttag tcggaggaat tatttttagt ttgttaggta tcttacaata agggaataac
     3481 caataaattt ggttgaataa tataaataag gaggaattca attgatgaat tcaaaagaaa
     3541 ttgttaaaga aatcgtagcg aaaaaagagg taaaatcaat cgtatttgtt ggctgtggcg
     3601 cttcaaaagc ggacctttat cctgcaaagt attttctaga ccaaaatgcg aagcagttac
     3661 ggattagtca ttacacagcc agcgaattta actatgcaac accagcatct gtagatgagt
     3721 caactgtcgt tatttcagct tctttaggag gtacgactcc agaaacagtt gaagctaacg
     3781 cagtagctaa agaaaaaggt gctacagtta ttagtctaac acgaagcatt gactcaccgt
     3841 taacaaaaga tgcagattat gtaatttatc atcgatttgc agagaactat gctgctaaat
     3901 tagaaaaaac aggctactgt ttagaattgg cagtagaact tttacatcaa gtagaaggtt
     3961 atgaccatta tgaagcaatg caagaaggat ttgctactat ctatgatctt gcccaaaaaa
     4021 gtgcagaatc agctcgtaaa gatgcgaaga aatttgctga acgctttaaa gatgaaaaag
     4081 ttatttatgt aatgagtagt ggtgctacac atgaagtagc atactcaaca tcaatttgct
     4141 tgatgatgga aatgcaatgg attaattctg gtaccttcca ttcaggagaa tttttccacg
     4201 gaccattcga aattgtggat aaagacgtac cgtttatatt attaatgaat gatggaaaaa
     4261 ctagaaatat agatgctcga gcattaactt tccttgaacg ctttgatacg aaaacagaag
     4321 ttgtagatgc cttggattat ggcttgtctt cacatattgc taaagaagtc ttagactact
     4381 ttaacccatt cgtgattact gcagtattta gagtttatgc agaagaattg tcagaagcaa
     4441 gagaacatcc attaactaag agacgttata tgtggaaact aacttattaa aaaatagatc
     4501 aaactaaata aggtggcttg agaaattaag ccaccttgtt ttcttcaatg tgattttata
     4561 catatggtaa aataactagt acatattgtg aaagcaggtg gcaagatgca gcgattaaat
     4621 gactatcttt atcaactaac attgcttttg aaatctacgc cttcagataa ttattcagct
     4681 cgcagtattt cgcaagcttt acagttgaat agaagtacaa tcagtagcta cttaaatgaa
     4741 ggtgttcgtg aagggctttt cataaaagta aaaagttacc cggttttatt tttacataga
     4801 acagctcttg aagaactata tatcacactg aataactccg aaatagaatc aattgaaagc
     4861 ttgttaacaa tttctcaaaa acccgcatta gatcaagtaa ttggctctaa aggaagtttg
     4921 aaagaagcaa ttgatcaaat taaaacggca gtcttatatc ctggaaaagg gttgccttta
     4981 ttattgatcg gcgctagtgg atcagggaaa acctttctag cgaataaaat atatgaatat
     5041 gcagtagaag aaaaggtcat tcacacatcg gcacctttca tagattataa ttgtgcacaa
     5101 tatgtgagca atccagagtt actatcctct gctttatttg gctacacgaa aggagcattt
     5161 accggagcaa gccaggagca tactggtttg ctagaaaaag ccgatggagg agtattgttt
     5221 ttagatgaag tgcatcgctt atctgaagaa gggcaagaga aattatttac atttatggat
     5281 acgggtgaat tctctcctat gggggataat agtattcgga aaaaagccga tgttcgatta
     5341 gtctttgcaa caacagaaaa tatatatacg actttcttac caacttttct taggagattg
     5401 ccagttattg ttaatctacc tagatttcaa caacgtccgt cgtttgaacg tctgtcattg
     5461 attgatgaat tttttgtttc tgagagtaaa attttagcta aggaactatc tgtttctgat
     5521 gcattgattc atttcttaat gaatagtgat tttgaaggta acgtggggaa tattaaaaat
     5581 attatcaagt atgcttgcgg aaacgcatat atccaccaga agagtcagca aacaattcaa
     5641 gttaaattat tggatatgcc tttagagtac agttataaat tcaaggagca actaaataaa
     5701 cccagaaaga aaagagcaga tcgaatctac tatccagaag aaacacgaca agtgcaacta
     5761 gaaagaaaca atcagctttt tgttcaattt tttgaccaat tacttactgg attctctgaa
     5821 gtgattgaaa ataacaagga tattaaacta tttttagaag aaatggttac caaggtaaca
     5881 caagtaatgg atacgcttat ttttcaagag gattacgaaa aagagcagtc tttatacgcc
     5941 attctaaact atcatattcg tcaaagtatt gattttatgc gagaaagcta cgggtttgaa
     6001 caagatggta accgggtagt cgctttggcc aactatctat atgtcaagga taatcaagtg
     6061 atttttgaaa atagtcttca agaaaagaaa accaaagatc agcttaacga attttttgct
     6121 aatgttcttg aaacccctta ttggtatgca aaaaaaatct taagcctttt atctgagtgt
     6181 ttagatcaac ctgtgtatga agaggacatt ttctttatta cgctatattt ttatagtttg
     6241 aatgtcacga atgttaaaac aggtattaag agtattgtgc tagctcatgg ttattctaca
     6301 gctagtagta tggcgaatgt tgccaaccgt atgctgaata agaacgtttt tcaagcttat
     6361 gatatgcctt tagatattac attagagaaa attgaagcaa aattgattca attccttgga
     6421 gattatcgaa cagattcagg cttgatttta ttggtagata tgggttcact aaaccaacta
     6481 ggcggtttat tagtgaatca tctaaatgga ccactgttaa tcattgatca tatcaataca
     6541 ccgttattat tggaagtcgg ccaacaagta ttaaacaata aaagtatcac cgaaatagga
     6601 gcagaaattg aaacaagtct tactttcaaa aagcagttac tacttcccaa gaaaaagaaa
     6661 aagaaagcca tcattacttg ttgttataca gggattggta gtgcgaccca gattcaggag
     6721 attttaacga aatgcttgaa agatgcagtt agagaattag taattattcc gtatgattac
     6781 aaaaagctgt tagaaaacaa gaattttgag actccttttc aattatacga tgtcatcatg
     6841 attgtaggta cagaagatcc caaaattaat cagattccat atattggatt agatcgcctg
     6901 attaatgggg aagcaattgg cgaatttgtt cattacttgg aaaaagagct gactattgaa
     6961 gtgaacgatt tacaaaaaga attaattttc aatttttcaa ttagtaaaat tgtcgaaaat
     7021 ttaaccattt tggatccaga taaggtgcta tcttttgtac aaaaagcaat taaagaatta
     7081 tcgaagttat taaattttga tttttctaat aatcagctgt ttttgttgta ccttcactgt
     7141 agttgtatga ttgaacgaat tgtacgtaaa gaaagtgtgg atgagcaaga ggacattgaa
     7201 gaatatatgc aagcaaatgg agaaataatg gataaaattc atagcgcttt ttttgagatt
     7261 gaaagggaat acacaattga aataccagat ttagagttgc gattattgag cgatattgtt
     7321 caaggatagg agtgaattcg aatgacaatg ggagtaattt ttgatatgga tggggtactc
     7381 atagacagcg agacatttta ttttgagaga agaatgaact tctttcaaga actggagata
     7441 gatccaggat caaaccagat tgatgattat gtggggaaaa cagaagctgc aatatgggaa
     7501 acattagttc ctgaggacaa atcattaaga gaggaacttt atccgaaata tgtagattat
     7561 cgaaaaacgc atccaattga tttcgctaaa gatttacgaa aagaagtgaa accgttactt
     7621 gacttcctca agaagcggga tataccattg gcactggcat cttcttctcc agttgttgag
     7681 atagagagta tgctcattca atgtgaactt actgaatatt tttcctttgt tataagtggt
     7741 gagtcattaa aacaaagcaa accacatccc gaaatatatt taaaagcgaa acagcaatta
     7801 gactgtgact tctttattgc agtagaggat tcaacgattg gtattaaatc agcaaaggca
     7861 gcaaatatct atacggtggc actaaaacaa ccaatgcaaa tcgatcagag tgaagcagac
     7921 atacaaattg aaagtctaca agaactggaa caaattgttt tagaaaaagg ttagtgaatt
     7981 aatggaaata aggctatata tctgtgctag ctattttgac taaccaaaaa aacttgctat
     8041 aattcaggtg tagtaaaagg tgctggtaac accttttacg aaaatgcttc ggcagtgata
     8101 atgggattgg ctttttaagt tttttatttt gttcgcaatc cctaaggtca tgtgaatgat
     8161 cctagggatt gctagaatat tttgcaacat tctatctatt tcagaacgta accttcccca
     8221 gttcattggg attcattagc atacaaccaa catttcaaaa agaaattgca ctacaccatt
     8281 attagctatt ttgactaacc ccaaaaaact tgctataatt caggtgtagg taaaaggtgc
     8341 tggtatcacc ttttacgaaa ctgcttcggc agtggtaatg agtgtggctt aaaggactag
     8401 tcctttaagc tttttacttt gttcgcaatc tctaagatca agttaatgat cgataagatt
     8461 gctagaatat tttgcaacat tctatttcag aacgtaacct ttcccattcc actgtgattc
     8521 attggcaacc ctcctcacaa ccaacgtttc aaaagaaatt gtaaagttgt tggggtacca
     8581 cgaccgaaca atactacacc tgaatgatag taagttgtct cattaagtat agcatatcct
     8641 ctttttgggt aaatagaaat cggttacatt ttttatccaa gaccaagttt tttcatttgg
     8701 tcttatttgc tatacccaaa caattccgca gtatagtcga cggtattgat tttatcaggt
     8761 gagtaataga aacttgtttt ccttccccaa aacattacct ataaaggaga aaaaatggta
     8821 tcgtaattat ataggtatag gatgaaattt ttttctaaga ggagggttgt tgatgaaaaa
     8881 tttacagtta aaatttattg ttgatccatc gattgtacgc tggtttcgga ttatcaatca
     8941 atttgagcgc aatcaacttg ccacaaaagg agaattagca aagagtaatc atgtatctga
     9001 acgaaccatt caaacagatg tcaataaaat gaaggactac ttttctgaaa gtgttcaatt
     9061 tgtttcaacg aaaagtgggt attcttttca gaaggaaaga ccggttcttt ttctcaagca
     9121 gaaggagcaa ctactggaga acgaaatttt atttgagttg ttagggaatg tcttttatgg
     9181 cgaattggaa gatatgacgg agctaattga tcgttttcat ttatctgaag cgacttttcg
     9241 tcggtatttg aagcagattc aaccagtttt ggaagaatac ggcttagaat tgagtcttta
     9301 tcctcttgat ttaaaaggag aagaggcaaa tattcgcaag ttttttaaag acttctatta
     9361 tgaaggggag atgacgcccc atactcttgt tccaccaagg gatcttcatg aacttgtgca
     9421 agctacattc tatgataaat ttgagtattt ctcgattgga acagggactt ctcctgctga
     9481 attttattat agtttgtata ttgcgattga gcgtgtgaga caaggaaaaa cgatagccat
     9541 tcctcaaggt ctgattgagc gagtaattgc tagtgagaat tttcgcttaa tgtattcctt
     9601 gaaagaagcg attcaaacaa attatcaagt tgctttatca gaagaggagt tttgttggtt
     9661 gtattta
//
LOCUS       DACDEG010000092         9574 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.096, whole genome shotgun sequence.
ACCESSION   DACDEG010000092 DACDEG010000000
VERSION     DACDEG010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.096"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            185..1183
                     /locus_tag="IV142_002289"
     CDS             185..1183
                     /locus_tag="IV142_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8485896.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="IV142_002290"
     CDS             1295..1804
                     /locus_tag="IV142_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP8485897.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="IV142_002291"
     CDS             complement(1834..2493)
                     /locus_tag="IV142_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP8485898.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="IV142_002292"
     CDS             complement(2495..3259)
                     /locus_tag="IV142_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP8485899.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3944)
                     /locus_tag="IV142_002293"
     CDS             complement(3267..3944)
                     /locus_tag="IV142_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP8485900.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3981..5366)
                     /locus_tag="IV142_002294"
     CDS             complement(3981..5366)
                     /locus_tag="IV142_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP8485901.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5576..5660)
                     /gene="ffs"
                     /locus_tag="IV142_002295"
     ncRNA           complement(5576..5660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IV142_002295"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="IV142_002296"
     CDS             complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="IV142_002296"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP8485902.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6305..7162
                     /locus_tag="IV142_002297"
     CDS             6305..7162
                     /locus_tag="IV142_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP8485903.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7243..7578)
                     /locus_tag="IV142_002298"
     CDS             complement(7243..7578)
                     /locus_tag="IV142_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485904.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7645..9015)
                     /locus_tag="IV142_002299"
     CDS             complement(7645..9015)
                     /locus_tag="IV142_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP8485905.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9093..>9574)
                     /locus_tag="IV142_002300"
     CDS             complement(9093..>9574)
                     /locus_tag="IV142_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8485906.1"
                     /translation="YVEEYGMKSITARELAEFCGCSTYPIYTHFKSISGLKEKILEEI
                     TVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAKSDLDMDSLFKRPFFSYVKG
                     HTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWDKNEVTDEYLENILKEQSP"
BASE COUNT         2806 a         1971 c         1615 g         3182 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgcta tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt
     3481 tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg
     3541 taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct
     3601 tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat
     3661 cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa
     3721 tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc
     3781 actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct
     3841 gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct
     3901 gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc
     3961 ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc
     4021 acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga
     4081 aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg
     4141 attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag
     4201 accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa
     4261 aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg
     4321 acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc
     4381 ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct
     4441 cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga
     4501 ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct
     4561 agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg
     4621 gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg
     4681 acatactgcc cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct
     4741 ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca
     4801 atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa
     4861 cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc
     4921 ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt
     4981 ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg
     5041 aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct
     5101 ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc
     5161 atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca
     5221 aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta
     5281 cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc
     5341 gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc
     5401 ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag
     5461 caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat
     5521 ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata
     5581 atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca
     5641 agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga
     5701 ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat
     5761 catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc
     5821 gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa
     5881 ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa
     5941 ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt
     6001 gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc
     6061 tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat
     6121 ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc
     6181 aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca
     6241 tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa
     6301 caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc
     6361 agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca
     6421 agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga
     6481 tcaaaaaaaa cacccatggt atcactatct ttttaaagat gcttcttgtg ttcaagaaaa
     6541 aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg
     6601 gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac
     6661 agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatgctactg aaatcaacgg
     6721 tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt
     6781 ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac
     6841 agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca
     6901 ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat
     6961 caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag
     7021 ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt
     7081 tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt
     7141 attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa ttttgtcaca
     7201 accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca ttttttcaac
     7261 aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat caccataaac
     7321 agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag aatccaagtc
     7381 actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa atttgtcata
     7441 gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca ttacttttcc
     7501 cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc cgattgttac
     7561 ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca tagcatagac
     7621 aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga aaaagttctt
     7681 tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata ctagcaaaac
     7741 ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt gccattcctt
     7801 ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg ctaatgcata
     7861 gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt
     7921 taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt tgaacactaa
     7981 aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga gtttttcctg
     8041 ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat gcatctgatt
     8101 ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt tatgaggcca
     8161 tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc ctgaactaac
     8221 ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa gccagctaat
     8281 gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt
     8341 ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac agtatagcgg
     8401 agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc cttctcctga
     8461 acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga caagttctgc
     8521 gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat tttctgataa
     8581 aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc ccttcatttg
     8641 agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa ctttttcacc
     8701 gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc tatattcata
     8761 cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct cattgctgta
     8821 gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc ctgccggttt
     8881 tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc
     8941 ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg gtaacgttgt
     9001 cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt acgaaaaata
     9061 aaggtggctg atctactttt tcatgttgct gcctaaggtg actgttcctt taagatattt
     9121 tccaagtatt catccgtcac ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa
     9181 tcttgagaag aatcatctga gcgcaaactt cccaatgcat taagccatac caattcaagc
     9241 gacttttctt ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat
     9301 agcgaatcca tgtctaaatc agattttgca atgatttcac gaatactttc atggatagaa
     9361 aaaaaatctt tcaataagta gacaatgctt aaaacatcat cggaactgca ttcagctaag
     9421 tagttttcaa aataaacagt gatttcttct aatatcttct cttttaaccc gctgattgat
     9481 ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga actctgctaa ctctcttgca
     9541 gtaattgact tcattccata ttcttccacg tata
//
LOCUS       DACDEG010000093         9480 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.097, whole genome shotgun sequence.
ACCESSION   DACDEG010000093 DACDEG010000000
VERSION     DACDEG010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.097"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(113..472)
                     /locus_tag="IV142_002301"
     CDS             complement(113..472)
                     /locus_tag="IV142_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP8485907.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(502..843)
                     /locus_tag="IV142_002302"
     CDS             complement(502..843)
                     /locus_tag="IV142_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485908.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(840..1514)
                     /locus_tag="IV142_002303"
     CDS             complement(840..1514)
                     /locus_tag="IV142_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8485909.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2310..3608)
                     /gene="asnS"
                     /locus_tag="IV142_002304"
     CDS             complement(2310..3608)
                     /gene="asnS"
                     /locus_tag="IV142_002304"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP8485910.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(3648..4838)
                     /locus_tag="IV142_002305"
     CDS             complement(3648..4838)
                     /locus_tag="IV142_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8485911.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(4859..5386)
                     /locus_tag="IV142_002306"
     CDS             complement(4859..5386)
                     /locus_tag="IV142_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8485912.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(5433..8222)
                     /locus_tag="IV142_002307"
     CDS             complement(5433..8222)
                     /locus_tag="IV142_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8485913.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(8369..8569)
                     /locus_tag="IV142_002308"
     CDS             complement(8369..8569)
                     /locus_tag="IV142_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8485914.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9021..9341)
                     /locus_tag="IV142_002309"
     CDS             complement(9021..9341)
                     /locus_tag="IV142_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485915.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
BASE COUNT         2586 a         2016 c         1717 g         3161 t
ORIGIN      
        1 taaaagacgt aaaagtcaca tagaagaact tatacaggaa aacgcataga gtcaggtgaa
       61 atacatgtat cccacctggc tctatgcttt atctaacctt tattattctc ttttatacaa
      121 tttctgcacc gaatggcatc aacgccaatc gagcaagttt gaaagattgc ttggcaaaag
      181 gtatccctat gatcgtgata catagaagga tgccgctgac gacatgtgcg agagcaattg
      241 gtaggcctga aacgatcagc cagatgatat taacgatcaa tgacatgcca gactggtgat
      301 aaattacttc tttgccaaaa ggcgcaaaag aaagggaggc gatcttaaaa cattgcatac
      361 cgattgggat acctatgatc gtgatacacc aaagtatccc cgctgctaac cagctaagac
      421 caccgattag tccgccgaat ataaaccaga tgacatttcc taaacaactc attgtatttc
      481 tccttctatt actgtttttg attaaaactg ggcaaaaatt gattttattc ttcttttttt
      541 agcttccagc atcaagtatt ctgcaaattt tttttggatt tcttccgtat tttcttttgt
      601 tactaatgtg tgttggatta gttttcctga tggtcgctga tccaatcgtt cttctgactg
      661 ttgcacgtcc gcttgcgcaa atccgataat ccgtttttct tttgttacag caacgagcaa
      721 tagtttattc ttatcattta ggtcagctgc cgtttttgct tttacgaatg attctggatg
      781 atcagtaatc agctgattga tttgaggtaa atcttctttt actgcccacc ttatttccat
      841 catttttcac ttttctttcg ctcattttta tctccactaa aaaaatcgac tgcgacaatt
      901 gcatctatcc agctttgtgc aatgatctca tgtccgccaa tcgttgggtg gaccccatct
      961 tctcttgtat atttcgaaaa accataagca atccctaaag cattgaatat accatctaaa
     1021 ggaaccagca atgcgccata ttccttagca agtttacgaa caatttggat tttagggtct
     1081 aagtctttac gccagccatg acggtcgtcg gttataggaa gtacaaacgg ctccatcaag
     1141 atgatgttgc agatttccgc tgtttgaagt tcatttagta attttcgata aacggattca
     1201 aattgctgag cagatttctc agttccgaat atagcctgat cgctagtatt atgccaggta
     1261 tcattgattc cgatcaaaat cgatacatag tcaggttgta aagctaaaca atcttcttgc
     1321 cagcgtactt gcagatctgc aattttgtct cctcctatcc cacggttcat gcattcaaat
     1381 tccatcgttg gatattggga gattagtttt ccggcaatca aaagtggata acctttacct
     1441 agatcagttg cttctgctcg gtatcttcct gcgtctgtaa tgctatcacc gataaataat
     1501 atacgttttt tcatttgtca aaaactcctt tttgaataaa acgatgattt tcttccttat
     1561 attatcccat aagccctatc aagacgaaag agaaaatata tgtataaaaa atgacaccag
     1621 cctagttcct tcaaacaatt gaatgaacgc tcatcaagtg tctagttttt tatgctattt
     1681 ttttcttaac gtgccggatt ccccttgatt ccttcgttgt ttggtccaga gagatcgatc
     1741 acacccaagc ttccgtcaat cagaccactg atgacgccat cgaaacatat atttataggc
     1801 tcatctaaga cttctagtaa atgtcccttt actaatatct cttctagatt gcttgggata
     1861 gattggtcat aactttatct ggtccgatga tttcaagggt gaattgaact tacatatgga
     1921 ttattgcatg ctgaaaatac ggacctactc catcttcgaa tgttactatt agtttgtttt
     1981 caggtgctag atgctgcttt atttcaacag cagaagcatc atcaaatcag atttccattt
     2041 gaattacctt ctttgtaaat gtttgattga ttgcgatttc gttatagaac cttgtgaatc
     2101 aaatagcaat ttcttttgct ttcttctctt gtctgctgga aacttccatt atcccgtatt
     2161 taaaaaaaaa gaactgcgag agttcgcagt tctttttttt aaataaacgt tgctctccgg
     2221 ccacaagtca caatagtcat caaacatgaa aaactgtttt ttgaaattac tccttgtgac
     2281 tcggagctga acggcaggcg cacaacctct tatggataaa ttcggttcaa caaacgtggg
     2341 aatggaatag cttcacgtac atggtcgata ccagcaatcc aagtgactgt tcgttcaaga
     2401 cctagaccaa aaccagagtg cggaacagaa ccgtatttac gtagatcaag ataccatgaa
     2461 tattcttttt catccaaacc gtgattacgg atttgttcta gcaagaaatc atagtccgtt
     2521 gcccgttcgc ttccgccaat aatttcaccg taaccttctg gtgcgatcat atctgcacaa
     2581 attaccacgt cttctcttgt tggatgtggt ttcatgtaga atggcttgat ggcttttgga
     2641 tagtttagga taaagacagg ctgttcgaat gaattcgcaa tgaacgtttc atgaggtgaa
     2701 ccaaagtctt cgccccattc gatgtcatca aatccatttt tcttcagtaa ttcgattgca
     2761 tcatcgtaag taatacgagg gaatggcaat tttgtataag tttttaagac ttctttgtct
     2821 cgtcctagta catctaatgc gtattcgcaa tgatctaaaa cattttgtac taagaatgct
     2881 acatattgtt cctgtacttc taaactttct tcttgatgag tgaaagccat ttctggttcg
     2941 atcatccaga attcgatcaa atgacgtcgt gtttttgatt tttcagcacg gaaagtcgga
     3001 ccgaatgaaa agacttttcc gaaagccata gcagcagctt ccatgtataa ttggccactt
     3061 tgagataaat aagctttttg atcaaagtaa tttgtttcga atagatccgt agtgccttct
     3121 ggtgcagaac cagtcaagat cggcggatca attttgacaa aatgatgttg attaaagaat
     3181 tcatacgttg cacgaatgat ttcatttctg atctgcataa tcgcatgttg tcttgaagaa
     3241 cgcagccata aatgacgatg atccatcaag aagtctgttc cgtgttcttt tggtgtgatc
     3301 ggataatcgt ggctctcgct taccacttcc acatttgtaa ttccgatttc ataaccaaat
     3361 tttgaacgac tgtcttcacg gatttctcca gttactaaca cggatgtttc ttggtgtaga
     3421 tttttagctg tttggaaaac ttcttccgac acttcgcttt ttacaactac accttggaaa
     3481 aaggctgttc cgtctcttaa ttgtaaaaat gtgatttttc cgcttgatcg cttatttgcg
     3541 acccaagcgc cgattgttac tgtttctccc acatggtttt tcgaatcgat gatttgaatt
     3601 tgttccacgt atgactctcc ttttatattc cttgaatttc ttcaggatta attttgactt
     3661 ttactctcaa taaattgctt gattctgcgt accgcttctt ctaacgtagc gcgatcagta
     3721 gcataactca tgcggacatt ttctggtgcc ccaaagcctt cgccagtcac aagcgctacc
     3781 cctgtttctt ctaaaagatc ttctacccat tgtgtgacat tttcatattt acacatgcgt
     3841 agcgtttctt ttacattagg aaaaagatag aaagctcctt gcggcttttc aagagaaaca
     3901 cctggcagtt ctgccacaag cggatataac gtattcaggc gttcttcaaa ggcgttccgc
     3961 atttcttcta ctgtatcttg ctcacctttc aatgcttcaa cagctgcata ttgactgact
     4021 gctgtcggat tactggttga ctgggaagca acagcaatca ttccattgat gatctcagaa
     4081 ttccccaccg cataaccaat gcgccagcct gtcatcgcat atgttttaga cacgccattg
     4141 atgatgatgg tctgattttt gattgcttct gaaatcgttg caattggagt aaactcattg
     4201 ccattataaa ctaaacgacc ataaatatca tcagcaacta tcaaaatatc attttttact
     4261 gcccattcac cgattgcctg aagttcattc ttgctataaa tcattccagt cggattcgat
     4321 ggagaattga tgattacggc ttttgttttt tctgaacgtg cttgttctag ctgttcaaca
     4381 gtaactttcc agctattgga ttcttcccct ttcacaaaga caggaagtcc ttctgccagt
     4441 tttacttgct caccgtaact gacccagtaa ggcacaggaa tgatgacttc atcttgcggg
     4501 tcaaggatcg tttggaaaag ggtatacaaa gcaaatttcg ctccgtctgt tacgactgtt
     4561 tgtgaaggtt catagacaag tccatagttt ttcttcagat aatcaacgat tgcctgtcga
     4621 agctctggga ttcctgctgt tggtgtataa tagctagctt tcccattgcg gattgcttcg
     4681 attgctgctt cttggatgtt ttctggtgtc gcaaaatccg gttctcccac agtcaaactc
     4741 agcacatctc tgccttttgc ttttaatgcc ttggcctttg ccgcagctgc caaagtgaca
     4801 gacggttcta atcgttttgt tctttttgat aactccatcc cattcattcc tttacctttt
     4861 aaattccctt gattgtcttg acttcatctc cagattcaaa gccaatcagg taataattca
     4921 gtttcccatt tgtttctttt cctactactt cccacacagg ctgatcatca tacatcccta
     4981 aagcagcttt ttcgatttgg atttcaggat gggccgcagc aattgtctgt tttgcttcgt
     5041 tttcagtcag tccctttgtt tgatccaata cttttacttt ttcaccagat ttaggaatga
     5101 taacggcgat ttcttttcct ttatcatttg tacctgtcag tgaaaagtat gtcttctttc
     5161 gagtaaacca ataaaaatga tcggcttcct caagatttgc atatttcttt gcgatttcta
     5221 ctgcttcttt cttcgcctgc accgccggac gactggaacg caagtaaaaa atcgagctga
     5281 tcacgatgac cgctaataaa aatacgatca agccaagtaa cacgcgatta atcgtttttt
     5341 cctttgtctg ttttttcttc aagggtcgtc gctcctttat cttcattcct tttcattata
     5401 gcagatttta attatgaaaa aagacagctc ctctaacttc cttcttcatt tttctttaag
     5461 aattcatgga tgtcctgata gatttcctcc atagctagct ctttgatcgg tacttttttc
     5521 ggtaatgcat ggatgatccg tgcaccatat tttgcagtga ctagtcttcg atccaaaata
     5581 agcaatacgc cacgatctgt ctccgtcctt atcaagcgac ctaatgcttg gcgtaacttc
     5641 aaagctgctt taggtacgga ttcctggtaa aaaggattga tcccctctga ttctaaataa
     5701 gcgttcctag ctttgaccat cgggcgaagc ggattatcga agggtaaacg tgtgactaca
     5761 acgatctgca aagcatcccc aggcaaatct acaccttccc aaaagctgtc agcgccaaac
     5821 aatacgcttt tttctgacaa aatgaagcgt ttcaaaagct tctcccggct tccgccaaac
     5881 ccttgagcta gtatttctcg tcctttttct agcatagaca gatgcatttt ctgatagacc
     5941 ttctgcaaaa catcatgaga agtgaataac acaagtattg gccgatcttc ttgttcgatc
     6001 agacctgtaa gtgctgtctc taaataatcg atatatgctt gattgctgac ttgatggacg
     6061 gcaggtgcct cttttggcac atagatcctt gcctgtttct cataatcata aggagaggca
     6121 atcactttaa gaggtgctgc ttcaatgcca agccgcttag agagatagtg tcgattaggg
     6181 ccgatcttca gcgttcctcc aatataaaga atacgatcat atcgaggata ccaaacagtc
     6241 gacggcaaca tagccgcttg aaaatcatgg agatgaaaaa tcatctgctg cgctgtgttc
     6301 ccaggaacga accaatggat caaatgatct cgccagtctt ccagccattg atccatcagc
     6361 aagtactgct tgtctaattg atcaaataag ctatataaac ttgctaaatc gatccgcatc
     6421 tggtttgtcc agctttcttg gataatctca aaagtcagac gcatttgtga ctgtagctgc
     6481 tggatctcct gataaaaaag ctgcaggtga tcaatggctg tcccagattc cagtggcagc
     6541 tgatccaaca tctcttttgt catcatgact tcttctggat actgtccgtt aggttgcttg
     6601 ccgctcacta aactcattaa tccttctgcc atacaaatct gtgcttcgca tagcgcagaa
     6661 agttcttctc gataaatcga taaccagcgt aaggcttcat gttctttcgg taaatagctt
     6721 tgtattcggt caaacagctg atttggttct tctaattgct ggatttgttt atggaaatag
     6781 ttaatactta atcgtttgtc atttactttt tcagcgattt ccggcaaatg atgtacttca
     6841 tcgatcaaca aaaatggact agctggaagc aatggttctt ttcgctgtgt ttcttgtatt
     6901 aaaaacgcat gattgacaat caaaaaatta ctttgcctga ttttcttttg caagaaacga
     6961 acaaagtctt gttcgtaaaa tgactgattt cttgctaaaa atccaatacc tctgtgcgcg
     7021 atatctgcaa agaatgaatg gttcaaattt gttaaatgta attcatccaa atcaccggtc
     7081 ttcgtttgcg tcagccaaac taagaccgcc atttgataca aagcatattg tttttgttct
     7141 ggcggttcca caagtgtgcc tttaaaacgc tgtaaatcaa tatagtggcg atagcttttg
     7201 ataattacag gttgtatggg ctgatctagc aattgattca gcaatggaat gtctttttct
     7261 atgatctgct gctgcaaaac tagcgaaacg gtactgatga cagcgggttt ttcaggtgta
     7321 gccaaataac tcaatggcag cagataaccg attgttttac ccattcctgt agctgcttcg
     7381 atcatcaaat cttttgattc atcttttgtg aaatggtcgt agaccgcatt catcagtctg
     7441 ttctgttctt ttcgatacat cagtgtttta ccaaacattt tttcttttgc ttgttttttt
     7501 cttggatatg tccgctcgcc ataatggacg gatgtgaata gttccacttc ttttttctgc
     7561 aaagctagcc catcgacgat ttctaatgag ggatcaagtg gctctggttt ttctttcatt
     7621 tcttcaatca catgataaat gaaacgactc gtatccatac ctgtgtgtct acttaatcgc
     7681 gcaatctgct ctaacgtgat gattggcaat tttctcatcc gttcttcgat cagcaaaaga
     7741 agttgacccg tcacttcggc atcactatct gcctgatgag gattctcatg aataaatcca
     7801 aggctttctg acaaatcagc taagcgaaag cttggttccg tcggcaagaa aatttgtgca
     7861 agttctactg tatcgatgcc tggtatcttc aaactaggaa gtccgcatcg catgagttca
     7921 tggttcaaaa aattataatc aaaataaata ttatgcgcga caaatatggt gtctgccaaa
     7981 agattgtaaa tcgtttgggc gacatcttcg aaataaggcg ctttttggac gcgttgattc
     8041 gtgatccctg ttaaatgctg gatttgtttt gaaatcttcc gccctggatg aatatcgatc
     8101 gaaaaacgtg aagtgatccg acctgattca atcatgacgc agccaaattg gatgatccga
     8161 tcttcttttg gattcgtccc agtcgtttcg atatcaacga ctgcataagt tcttggtttc
     8221 aaaatatctt cctcttctcc tgcctctaat gttcttttga aattatacta caaagtgcag
     8281 aaaaatactc tcatcgcttt ttaaagaaaa tagacaaaaa cgcccagaca cgcaaatgtc
     8341 tgggcgttta ttttttagta taggttaatt aagctttagt aacgtttgtt gcttgaggtc
     8401 cgcgttggcc ttcttcaaca tcgaaagtta ctgcttggcc ttcttctaaa gttttgaatc
     8461 cgtcgccttg gatagctgag aaatgaacga atacatcgtt tccgttttct gcagtgatga
     8521 atccaaatcc tttttctgag ttaaaccatt ttactgtacc tgtttccata ataaaaatcc
     8581 tccttgtgta tcaacacata ttttgcaatt acttgaatgg tgtatatctg gaagatgttt
     8641 atttgaaaca agctaccaca gattaccgaa caaaaactac tcctatagta tatcataccg
     8701 tttacaaaaa agctagagtt tttcgacttt ttttattaaa aaaagttttc tcagcgatta
     8761 cataaagtga aaaaccgaac tttttttaaa ataaacgaca tattgttcag ttggtgtttt
     8821 tttgctctga tgttcttctt taaactaaaa aaattattct ttctgactac ttatcggttc
     8881 tgtttcaacg gtcgaggctg gggaagattc tttagtaggt ctgatgttct catggtattt
     8941 ctgatagcct atgacagtta ccagaagaac aaggcaaacc aatacgatca acgagattag
     9001 tttttctttt ttgctcatat ctatacctca taaataacag gatgatgtcc atcatatttc
     9061 tgagtatgag taaaagcaaa agctgcagct gtagaatgta agaaatgcca gcaactttca
     9121 taatcacagg tcggacagta ccaaaagtaa ccaaatcttg gctgcaaaga ccgttcttct
     9181 gtttcaggaa tcgcagaatc aagatctgtt gtctgtacat aaacattttt gtacagacgt
     9241 gtgcctgtaa taggagaccg ctgtgtttct tcagcaaaaa ctggctgact tgttaaactt
     9301 agggatagaa acagcaaaca ccataacagt ctttttttca taaagacctc cttatcgata
     9361 agtttttccc aattaaaatc atatctcaag aacgcccgtt attcacattt tgttcataaa
     9421 tgatgttttt tgtatcataa tcgtcaaaaa gaggtcgtga ccacgtcaca acctcttttt
//
LOCUS       DACDEG010000094         9456 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.098, whole genome shotgun sequence.
ACCESSION   DACDEG010000094 DACDEG010000000
VERSION     DACDEG010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.098"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..641
                     /locus_tag="IV142_002310"
     CDS             <1..641
                     /locus_tag="IV142_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HAP8485916.1"
                     /translation="VLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEYLKER
                     GLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSRSFREAVKG
                     IFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRLKSTN
                     LIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            complement(795..1325)
                     /locus_tag="IV142_002311"
                     /pseudo
     CDS             complement(795..1325)
                     /locus_tag="IV142_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
     gene            1397..2689
                     /locus_tag="IV142_002312"
     CDS             1397..2689
                     /locus_tag="IV142_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8485917.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSECRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
     gene            complement(2901..3743)
                     /locus_tag="IV142_002313"
     CDS             complement(2901..3743)
                     /locus_tag="IV142_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8485918.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
     gene            complement(3780..5246)
                     /locus_tag="IV142_002314"
     CDS             complement(3780..5246)
                     /locus_tag="IV142_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8485919.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            complement(5302..7146)
                     /locus_tag="IV142_002315"
     CDS             complement(5302..7146)
                     /locus_tag="IV142_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8485920.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            7748..8698
                     /locus_tag="IV142_002316"
     CDS             7748..8698
                     /locus_tag="IV142_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8485921.1"
                     /translation="MKNDIVTYANTKCEEIGISNLEECFFDTNDLSQFLRTGRTNASK
                     LANDLVAKNVLFKINTRPVLFAIKERFEEHFLIPVKDVYESISELKTDLEPNSTDSIL
                     QKMIGYDMSLSDAVEQMKTAVFYPGRGLPMMITGDTGVGKSFFVHTAYQFMKKVNVIH
                     ENAPFKVLNCAQYHNNPELLSGILFGYVKGAFTGADEDRNGLLQDADGGVLFLDEVHR
                     LPPEGQEKLFVYMDGGTYSRVGETIHHASNVRLVFATTEGKEDFLETFMRRIPIHIHI
                     PNIEERGILEKKQIIENTLFSESKNFSKAIDVSPKVTSVA"
     gene            complement(8743..9376)
                     /locus_tag="IV142_002317"
                     /pseudo
     CDS             complement(8743..9376)
                     /locus_tag="IV142_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
BASE COUNT         2939 a         1900 c         1575 g         3042 t
ORIGIN      
        1 gagtactctc aaaaagctgt catatagcga ttggaataac caaagatggc gaccgtgaaa
       61 ttatcggctt catgattcaa agtggcgaaa gcgaagagac ctggacaaca ttttttgaat
      121 acctaaaaga acgcggttta caaggtacgg aactcgttat ttctgatgcg cacaaaggat
      181 tagtctctgc cattagaaaa tccttcacca acgtaagttg gcaaagatgc caagttcact
      241 tcctaagaaa tatctttacc accattccta aaaaaaattc aagatctttc agagaagctg
      301 ttaaaggaat ttttaagttc acagatatta acttagcgcg tgaggctaaa aatcgattga
      361 ttcatgatta tatcgatcaa ccaaaatatt caaaagcttg cgcatcattg gatgatggat
      421 tcgaagacgc ctttcaatat accgtacaag gaaattccca caatcgacta aagagtacca
      481 atctaattga acgactgaat caagaagtac gcagaagaga aaagattatt cgcatcttcc
      541 ccaatcaaac atcagccaat cgcttaattg gagccgttct tatggacctg catgatgaat
      601 ggatttattc ttcaagaaaa tacatcaatt ttgataagta gaaatggtaa aaacattgta
      661 tagcatttta cacaggagtc tggacttgac taacatttcc atttcattta cctctttttc
      721 gtttaattat actaaagttc tatccaagcg acaagctgtg tataataaaa atatacccgt
      781 ggtgctagtt aattttaata ccctaaagaa tatctcaaag tcccttcaaa atgttgctgt
      841 gctcggctta aaagaatact gtacgataat aatatgcttt caaacttact ttctagccct
      901 tgaatagaac gtgatttgaa attttgaaaa cctagtcttt cgagagaaga aaaaacagtt
      961 tctactgctt ttctttgtct agtaagtaat cgatggtcta tcttccctag tttatccatg
     1021 ttttttcgtg acggaactaa gactgtaatg cccttaaggg caagcttttc atggatttta
     1081 tggctgacat agcctttatc tccaaggata tttggaaggt ttgcttcttc cgataaagtc
     1141 tctaaaactt tcacatcatg aacactagga tccgtgattg aataagtaac tggaaagcct
     1201 gttttagtga cgataatatg aattttaaga ccataaaaat aagcgttttt cgttgagtta
     1261 tagcccctac agctctatac tttttgatgt tggtggatga caccagcatc ttttttattt
     1321 tccaaaacat atagaaaaga caccttgaaa tcctttagaa ttaatttggc gaaccaaccc
     1381 aaaaggagga atcaagatgt cttacactca ccttatcaaa gaaacattag atattctaga
     1441 cttatcagtt acttttaacg aaaattgtct cacaaaagaa aaatacaaag ggcaaatctg
     1501 tcatatttat cgtggaaact tgatttatac agcccaagaa tgcatccact gtaaacacca
     1561 gattgcttca gatattgtgc gttggggtac aacaaccgtc cggttgttaa tgaacgatgt
     1621 ttctgaatgc cgtacctacc tcgaattgaa aaagcaacgc ttcaaatgca aagcttgtca
     1681 gcggaccttt gtcgcagata cttctgttgc gaaaaaacac tgtttcatca gtgagaaagt
     1741 ccgctggtct gtcgtgactc gtctgaaaaa aaatacctca atgacagaga tcgccgctca
     1801 gaaaaatcta tcggtctctt ctgtttaccg tattatgaag cgattttatc gtccgttgaa
     1861 tccttttcgg acaccgttac ctaaggtact ttgttttgat gaattcaaat cagttcgggg
     1921 tgtttcaggt gctatgagtt tcatcatgat ggatggacaa acacagcgct tgttggacat
     1981 tgtcgagaac cgacaactgc cttttttaaa acgttatttt agtcattttt cacgagaaat
     2041 tcgtgaagcg gtcgaatgga tcgtgattga tatgtacgct ccttatgtat cgctcgttaa
     2101 aaagctgttt ccaaaagcac agctgattat tgaccggttt cacatcgtcc aacatattgg
     2161 tcgtaccttt cgcaatcatc gaatcaaaga aacgaaccaa ctattaaaaa gcaaagaaca
     2221 aaaacactat caattaggta agcaattaaa aagatactgg aaacttcttc aaaaagatga
     2281 acgcaagctt gattatactc gacgcctttg gcgacctggc ttcaaagctc atttaacaga
     2341 gaccgatatt gtggatcgct tactgaaagg gagccctgct ttgcgtgtag gctatcagtt
     2401 atatcaagac tttctttacg ctgtaaagga acgagactac gtatcgtttg aagaactact
     2461 taccaataat atcatgcttc ccgaaggcta tcagacgacc ttgagaacgt tccagaaatt
     2521 tctgccacaa atcaaaaatg ctttacaaca aagttattca aatggcccac ttgaatgtct
     2581 gaacaaccat atcaaagtgt taaaacgaaa tgcatatggc tttcgtagct tttataattt
     2641 caaattgcgc atcatgattc gtcatggcaa cgcattgatt ttcaactaaa aaagatcaag
     2701 aggaactgat ccacttgatc tccaattatt ctaagatttc tctttaccaa catcatttga
     2761 caaagagccg agatttttta ttatcttacg ttaatccttt ttaaatgtct cttatagata
     2821 acaaacagct ggaaatcacc ttgtttttac tataatccat ctctaaagat tttaaaagca
     2881 tcaatagtat tactgagcgc tcacccatta gcatgtttca tcaaccgatt gatgtggaca
     2941 cctaggtata caacttcatc gttcggaatt gttttatgat aaactttttc aatgtagtct
     3001 ttgatcttag ctgcgctttc catggcagac gggtataaga tccacatttg attgtagaat
     3061 tcttcctcac catcatccaa caaagcatca gaataatatc tttctacaaa gaaacgtaca
     3121 tgtgtaatga agcgggcata atggatcgaa ttagtatcga tatctttctg caacgaatac
     3181 cggaccaaat tgacgatccc gccgatcatt tttgcatact tgaatccagc gccgctatca
     3241 ggatcttccg attgtgcatt gatcaaatgg aaagcaatgt ttgaggcttc ctctttagga
     3301 agatcaattc catattgctc gttcaaaagg gtgagtgcgt acttcccaat ttgaaattct
     3361 ttgggatagt agatttttat ttcccagtac aaacggtttg caatatttac ccctttttgt
     3421 gcgcgttcaa ctgcaaaatg caggtggtct gttaaggtta aatagactcc aggattcagt
     3481 tttttatcta atcctttttc tgcttcaaaa acgatagtac gagtgatttc aaaaaattca
     3541 ataggtattt cttcaactaa ctctaatagt tgtgaagatt tcttttcttc aaaaggcaga
     3601 aatattttat ctactttatc tcctgatatt tcatccccag ctttctgtcc aaagcctatc
     3661 cctttaccta atgcaatcat ttctttgcca ttttcttcaa caagtaccac acttgaattc
     3721 aatacttttt ttattgaaat cactcattat ccccctatcg ctgcttaaaa agatcgagac
     3781 tactcgaata aggatgcacc gtttgtttcg atcacttctt tgtaccagtt gaacgaatcc
     3841 tttttcaagc ggttatatgt cccatttcct tcatcgtctg catccacata aataaatcca
     3901 tatcgtttag acatttgaga agttcctgca ctaactaaat caattggtcc ccagctagta
     3961 taagctatca aatctacacc ttcatcgatt gcttctttca tttgttggaa gtgtgcttta
     4021 aagtagtcga tgcggtacgg atcatggatt ttaccatcta ctacctgatc gactgctccc
     4081 ataccatttt ccacaatcat caacggtact tggtaacgat cataaagttc gatcaacgaa
     4141 attttcaaac cttttggatc gatttgccag ccccaatctg ttgaaggcaa atgaggattc
     4201 ttaaccccta aaacagtatt tcccggtgtg cgttctgcgt ttggatcagc tgattctgcc
     4261 atggacatgt aatagctgaa agaaacaaaa tctacggtat gtttctttaa aatttctcga
     4321 tcttcatctt ccattttaat ctgaattccc cgattttcca aatccttttc gattagagca
     4381 ggatattctc ccttaacttg aacatctgca taaaaattat tttctaagtt ctttttcaag
     4441 gtcagctcaa cgtcttctgg tgcgcaagtt aatggataag tcatcaattt cgttaacata
     4501 caaccaactt ggcttcctgg aattttttca tgagcatctt ttgtcactaa cgcactcgca
     4561 acaaattgat gatgcaatgc ttgatagata tcttgagctt cttgaccttc tgcgcttttc
     4621 tcttggataa ttccagctgt tgtaaatgga tgacggtgga tgctgtctat ctcattaaat
     4681 gtcagccaat attttacgta tttgccgaag cgatcaaaac aaacgttagc gaagcgaaca
     4741 aaatcatcaa ttactcgacg ctccacccag ccattatatt taactgctaa agctaatggc
     4801 atttcataat gagatagtgt cacaatcggc tcaattccta aacgttgcat ttctttaaag
     4861 acgttttcat aaaatgcaac ccctttttca ttcggttcca gctcttctcc tgtagggaac
     4921 aagcgagtcc aagcaataga gaggcgcaag gttttaaagc ccatttcggc aaataaagct
     4981 aaatcttctt tatagtgatg ataaaaatca acgccccttc tttttgggta ccatttatca
     5041 gatttgtctt cactagcttc ttttatagac tgcagggtga catgcatatg agcagcaaaa
     5101 tctttgttgt ctaaatttgg tttataagta gccatatccg ctactgaaag acctttaccg
     5161 tctacattcc aagcaccttc tacttgattt gctgctatcg cgccgcccca taaaaatcct
     5221 tttggaaaac ttgttgtgtt tgtcattatt ctattctcct ctattccttc attcaatata
     5281 ttttttttca ctatcttgtg tttaagctat cggactgata gctaagagat cactatcatt
     5341 ggttaccaat ttttctaggt taaatgaacg gatagtgtaa ttatccttat tggttaccac
     5401 acagataact gtggggtcga atcctgcttc tttgattaaa tcaatatcaa atgcaatcag
     5461 cttttgtcct ttggctactt tttcaccaga acgaacaaat gcctcaaacc cttttccttc
     5521 taactgaaca gtatcaatac caatatgaaa aagaagttct gcaccattag ccgtttttaa
     5581 accaatagca tgcttcgttt caaataccat tgtgatttct ccatcttcag gagctactaa
     5641 tatcccttct tcaggaatga ttgctactcc atcacctacc ataccactag aaaaaacagt
     5701 gtcttttact tctgatagtg agattacttt tccattcacg ggacttgaaa gaacagcatc
     5761 tgaaacaacg gccgtttcca tcttagtttc aacttcattg acaacttctt tatctttata
     5821 aagaaccaac gctgcagcaa aagcaaccac aaatgaaatc cccgatactg caaatgccca
     5881 aaccagattc attgggtcag ctttgtcagc gaacatcgtg atacttgcaa tcccaggacc
     5941 cactaaagca aaggcttgaa gtgcaacgat tcctgcaaat gctcccccga tcaggctaga
     6001 aaacataacg gcatataaaa cacgtttgtg cagaatcgtt accccgtaaa gagctggttc
     6061 agtaattccg aataatgcag atatacccgc agaaacagcc gtagatttca attttttgtc
     6121 ttttgttttg atactgacag caaagcatgc gccagcttct gaaatattgt gtgccaatga
     6181 agccgggaga taaagaagtt ctgcacccaa ttcactcatg gatgaaacag cgtaagggat
     6241 cattgcttta tgcatacccg ttacaaccat taatggcagt acagctgcta agactccggt
     6301 tgcaacccat ccaaattgat tgtacaaaga cacaatgata gaagagaaag ctgttcctac
     6361 attataacct aatggtccta gaagcaacaa agcaatagga actgtaatca ataagctcat
     6421 cataggtaca aagaaaatac gaattggttt tggtgaaact tttgtaaacc atttttcaag
     6481 ttgagcataa aataaaacag ttaagatggc tgggaatact tgagatgcat acgctatatt
     6541 atccaatcca aaagataaga atgtagcacc ttccgccaac atagctgtca tttcaggaag
     6601 gatcaatgca ccgacagcag atactgcaac caattgattg actttcaatt tattagccgt
     6661 agtcattgcc accagaatag gcaagaaata aagcggtgcc ccgccgacca aatttaaaat
     6721 ctgataggtt tgagattggt cactaatcag ccctaagata gaagctagta gcaacaagga
     6781 cttcaatact cctccacctg caatcgcagg aattaaaggt tggaaaatag aaataataaa
     6841 atctaataga actgaaccta atttttgttt ttgttttggt gcttcttcag tatttgaaac
     6901 attgtcacct aataacgctt tcacttcatc atacacttca acaacatcgt ttccaatgat
     6961 tacttggcat tgcacatttt ggcgaacgcc cataacgcca tccacttttt ctaactggct
     7021 catatttacc ttgctgttgt cttttaacgt aaaacgtaaa cgagttgagc aatgctccaa
     7081 atgggatatg ttttcttccc caccgatgta ctgcaatact tcttctgctg tttgtttgta
     7141 attcatggtg tttccctctt ttcttttttg agtgatgaac ttttgagtcg aagtggccat
     7201 cctcaacaca aaaaacaggc agaaactaaa taaattgaca acaaggcaaa cgtgtattga
     7261 aacgcataca ttgttattca acttatttag gtcctgcctg cattaccagt cacatgcctg
     7321 tttaagttat attcaattta acatattaga atgtaaacgt aaataataaa atgatttttt
     7381 aatcattttt tcattttatt cgttcaatat agttttcgtg ttctaaaagt tttgtctttt
     7441 ttctaagaat atcttccaac atatataact cttattctct tctatcttct agcagatgtt
     7501 ctgtcttttt aagacctagt ataaaaagaa tgggttcatt ttttctttct tgcaggatga
     7561 ggttttttat ataaacttta tttgatataa agaaaaggca ttcataattg tgctatacta
     7621 ctaaaaaatg ataatacagt gaattagatt agcgctttat taaattaaga ggacagcata
     7681 tcgatattgc ttcctttttt ttgctattta tttaagggat agaagtttag aaaggactgt
     7741 ttataagatg aaaaatgata tagtgactta tgcaaacaca aaatgtgaag aaattggcat
     7801 cagtaattta gaagagtgct tttttgacac aaatgacctg tcgcaatttt taagaacggg
     7861 aagaacaaat gccagtaaac ttgccaatga cttagttgca aagaacgttc tatttaaaat
     7921 caatactcgt ccagtgttat ttgccatcaa agagcgcttt gaagagcact ttttgattcc
     7981 ggtaaaagat gtatacgaaa gtatatctga attaaaaaca gacttggagc ccaattctac
     8041 agattccatt ctacaaaaga tgattgggta tgatatgagc ttatccgatg cggtggaaca
     8101 aatgaaaact gctgtcttct atcctggaag agggcttcct atgatgatca ctggagatac
     8161 tggagttgga aaaagttttt ttgttcatac tgcttatcag tttatgaaaa aagtgaacgt
     8221 tattcatgag aatgctcctt ttaaagtact taattgtgcg caataccata ataatcctga
     8281 attattgtcg gggatactat ttggctatgt taaaggtgct tttaccggcg cagatgaaga
     8341 tcgaaatgga ttgttgcaag atgcagacgg tggagtactg tttttagacg aagtacacag
     8401 attgcctcct gaagggcaag agaaactatt tgtttatatg gatggcggaa cttattctag
     8461 agtcggtgaa actatccatc acgcctccaa tgtacgttta gtttttgcga ctacagaagg
     8521 caaagaggat tttctggaaa cctttatgcg aagaatccct atacatatcc atatcccaaa
     8581 tattgaggaa cgaggtattt tagaaaaaaa acaaattatc gaaaatactc tctttagcga
     8641 aagcaaaaac tttagcaagg ctattgatgt ttctcctaaa gtcactagtg ttgcataaat
     8701 atagtcagaa tattctgaat tattattttc ttgaaaaaag taaaaaaaat cgtatcccaa
     8761 actttcatct acatttccag aaagatcttc atgttgataa tcaattcctg aatcaatgac
     8821 cgctaccttc acatccttag aacccttggt ttcttcccaa gcggatggca tgctaatctc
     8881 tcttaaatta gcttgtttac tccaattagg atcgttatcg atttcctaaa gaatcagttg
     8941 tatttggctc ctcaacatca ttcaacggct ctagttcaac cacatagttt ggctctgcat
     9001 aattgaccat atcagaagca ttaaatgttc ttactgcctc ttccacacta atttcttcgt
     9061 tcaatttcac taataatact tgatctcgct tattataatc gctttcattt gtcctaagag
     9121 tcaaatcttt ttccaccata acttttacac ccaataaatt ttgcggttta tttttctctg
     9181 ttgaaattct ttgtgtagcc tttaaactga caattagctc attgggtaca tactctattt
     9241 gagagtcatc taattggctc ataatccttt ctttttggtt agtatctgca gttacttgtt
     9301 tagtatgagc catgaaccca aacatactca ccccaacaac caatcctaaa tatattttct
     9361 ttttaaactt tttcatctac atctcctcct aatagtttta ttttagagtt agctctatta
     9421 cttatttact aactcaacaa cagattatca tacact
//
LOCUS       DACDEG010000095         9398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.099, whole genome shotgun sequence.
ACCESSION   DACDEG010000095 DACDEG010000000
VERSION     DACDEG010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.099"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..2202
                     /locus_tag="IV142_002318"
     CDS             <1..2202
                     /locus_tag="IV142_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP8485922.1"
                     /translation="LADGQKGDNRLKTLKEIQQIYPQIPESKGSFPGYGGIPITHYKK
                     YWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAERQSSMVVNDPKTELYVASKDE
                     LEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDIDTATQLTNTFTNTIYYDANAS
                     DNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNNLVELLSEIGGFNYYPDPENEF
                     KQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNILSTAITGLNPFTLTKIAKMTS
                     QSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHKGKKFHEERVKVGYRGFCEINFDC
                     HLEQGDHVCLKYNHQGKQYVAWYEFSQKVLKDEEGKIVYRKKRGETHLPELEKEAVLT
                     LDEKKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHIIVSIFLSQLYKELSTQCVKTK
                     GDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNMLWDLFIQSYQQLYAKYGEQNAA
                     IIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENSTINSLGMNTHINRNVDSDRIL
                     TPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNHGETNLPYAYQYIEDFNPSWDI
                     NDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGLHDQLEEKNEENMFYKIVQSFE
                     NETAKKLLIQFYEEQATDRLQEYVEKLIKNEEISEMIRKRLLLEAEKAVKE"
     gene            2199..4577
                     /locus_tag="IV142_002319"
     CDS             2199..4577
                     /locus_tag="IV142_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485923.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDITHDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTVE
                     DLTEKAKDTLEEKLDGTRAKEFAEAVKNPSEALEKQKEKASAVKEGLKDAYGEGQVNA
                     AVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQYRY
                     KSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMK
                     DSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLAESGETENASVQNIV
                     TKEAAPKPINKVHNGYRPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKP"
     gene            4638..5180
                     /locus_tag="IV142_002320"
     CDS             4638..5180
                     /locus_tag="IV142_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485924.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
     gene            5258..5590
                     /locus_tag="IV142_002321"
     CDS             5258..5590
                     /locus_tag="IV142_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485925.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            5591..6184
                     /locus_tag="IV142_002322"
     CDS             5591..6184
                     /locus_tag="IV142_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485926.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            6206..8230
                     /locus_tag="IV142_002323"
     CDS             6206..8230
                     /locus_tag="IV142_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP8485927.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            8230..>9398
                     /locus_tag="IV142_002324"
     CDS             8230..>9398
                     /locus_tag="IV142_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8485928.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP"
BASE COUNT         3298 a         1490 c         1961 g         2649 t
ORIGIN      
        1 ctagcagatg ggcaaaaagg agataaccga ttaaaaacgt taaaggaaat ccaacaaatt
       61 tatccgcaaa taccagaatc aaagggaagt tttcctggtt atggcggaat accaattact
      121 cattataaaa aatactggta tattcagacg gatacagtaa atacttgtat tgttggtact
      181 tcccgctcag ggaaaggcca gatagaagtt ttagcaacca ttgacaattt gtcgcgtgca
      241 gaaagacaaa gttcaatggt tgttaatgat ccaaaaacag aattatatgt tgctagtaaa
      301 gatgaactag aagctagagg gtatgatgtg tatgccttta atatcaccga tccactgcaa
      361 tcaatgagtg ataatccatt ggcactcatt gtgaaatact ggaagcgagg agacatagac
      421 accgcaacac agttaaccaa tacttttacg aatacgattt attatgatgc aaatgcttca
      481 gacaataaat actttaacga aaatgcgcag aaagctgtca atgcgcttat ttttgcgtta
      541 ttggaacaag cggatcaaac aggggattac tccaaattaa caccaaataa tctcgttgaa
      601 ctactttcag aaattggcgg ttttaattat tatccagatc ctgagaatga attcaagcag
      661 ctcaatgcct tagatgagtt catgaatcag ttaccccctg gaaatatagc aaaaaaacaa
      721 tatggggcaa caaaaattgg atctgataaa gcgaaaggga atatcttgtc tacggcaatt
      781 acaggattga atccatttac cctaacgaaa attgcgaaga tgacttctca atcgaccatt
      841 gattacaagt ccattggttt tccgaagtat ttggatctca aattacatga aagcctatta
      901 aatcaacgca ttgtcgtgga attttatcat aagggaaaga aatttcacga ggaacgagtc
      961 aaagttggct atcgtggttt ttgtgaaatc aactttgatt gtcatttaga gcagggagat
     1021 catgtttgct taaaatacaa tcatcaagga aaacaatatg ttgcttggta tgagttttct
     1081 caaaaagtat tgaaagacga agagggaaaa atcgtttatc gaaaaaaacg aggagaaact
     1141 catttgccag aactagaaaa agaagccgtt ctaacattag atgagaagaa aagtaaccta
     1201 ttagtagagt ctattcggat gcactatacc gataaaccaa tggctatttt tatgttaacg
     1261 cctgattatg atccatctaa ccatatcatt gttagcatct ttcttagtca gttgtataaa
     1321 gaattatcta cgcaatgtgt taagactaaa ggagataaat gtcaccgtag gattcatttt
     1381 attttagatg aatttggaaa tatgccaccg atggacaaca tggaaggaat catgacggtt
     1441 acggcaggtc gtaatatgtt atgggatctg tttattcaat cttatcagca actttatgcg
     1501 aagtatggag aacaaaacgc agcgattatc aaagaaaatt gtcagacaca tatttatatt
     1561 atgtcgacag atgataatac catcgaagaa ttttctaaaa aagtggggaa taagacggtt
     1621 gaacaagaga acagtaccat caattcatta ggtatgaata ctcatatcaa tcgcaatgtt
     1681 gattcagatc gtatcttaac tcctgaaaga atttctactt ttttagaagg tgaaacgatt
     1741 gtactcgctc ctttaaaacg acaagatttg aaaggtcata aaatacggcc gtatcctatt
     1801 tttaatcatg gggaaacgaa tcttccttat gcttatcaat atattgaaga tttcaatcca
     1861 tcgtgggata tcaatgatat agacatacat tgtacacatg cggacttgga tcttaaggct
     1921 ttacaaattg attatgatta tttcctacca gatgaatatg ctagaaatct ctatcatgga
     1981 ctgcatgatc aattagaaga aaaaaacgaa gaaaatatgt tttataaaat cgttcaatct
     2041 tttgagaatg agacggctaa aaaattgtta attcaatttt acgaagaaca agcaacggat
     2101 cgtcttcaag aatatgttga aaagttgatc aaaaatgaag aaatttctga aatgattagg
     2161 aaacggctgt tattggaagc tgaaaaggcg gtgaaagaat gatcattgga ggaattttct
     2221 ctggcatagc cgactggtgg tcaggtttat tcggagaagg ggatgcagat cccaaaaccg
     2281 taacgaaagt cttacaagat tatggtaatt atctgcagta tcaagatgta ttcagttgga
     2341 tttgtaatac ttttttatgg ggattgatca aagggttata taatctcaat actttattag
     2401 aaaaaacaat ttatcaaagc tttgatttaa aagacttatt gaacgccgct ggtgttaatg
     2461 aactatttca aagtttgatt agtaaggtat tggctctttt ttgtgtcgtc acattaattt
     2521 atttaggctt gaaatttttt atgagtaaac acccaccgaa agtcaaaaat atcttggtta
     2581 atttatttat ggccatgatc ttgatttttg gtggtagttc tttaatcgat gaagggttaa
     2641 atatcagtaa aaacttttat ggagacgtca ctgcagcaaa taaagcagat aaaacgggtt
     2701 cacctgcttt tcaaattatt caaaataatg tgattgatgt cagtacatta ctagcgactg
     2761 acccgaataa agtagaatcc ttaccaacag ataaaagaaa tgctttaaca gcgaaaaatt
     2821 tcaaaacagc gaatatcaat gggattatta cacctgatca agctaagaaa atgtctgaat
     2881 caagtgatat tagtacagta caaaaagatc ggttaaaggc attacagtat cgactagagt
     2941 tagacgataa tgggaaagaa atccctgttc aaattagtga tgaaggttta gcaaaatatg
     3001 tgtatacgtc aggctatcgt cgatacatta gtagtccagg gattattttg gctggggaat
     3061 cttctttgtc ggtagcctat ttatttattt tgtttacaat cgttacttgc attattgagc
     3121 tagtatttaa aaaattctat ttagtaattg ccgcgtcaac ggactttgag acaggacaac
     3181 ggatgaaaag tgcgatagaa gatatttctc aatccttctt gttattggcg tttacgattt
     3241 tagaattacg aatctataca ttaatgcttt caggtatcgg tgatttgcat gcggtaggaa
     3301 agatcaatgg attcttgtat gttgttgctt taattgtgct aactattgcg ctattcaaag
     3361 gctctcaatc ggttacgaag attttcggtg ttgatacgag tctgaaaaac ggcagcaatt
     3421 ctcttcttag catgtttgcc ttaggaaata ttgcgaaaaa tgtgggtagc ggagctaaaa
     3481 accttgcaag tgctggtaag agtggtttaa gccatttaaa tgatattcgt aagaatggat
     3541 ttggccatcg aaaagatatt acccatgata acggggaaga acaatcagac gataaagcta
     3601 caactgtacc taatagtaag aagacagttt ccgaaatgtt cgggaaagcg aaaaatggtg
     3661 tttctaaagc cgcagaaggt cttggttacg tcaatgaacg tgggttaaaa ggaacagttg
     3721 aagatcttac agaaaaagcc aaagacacct tagaagagaa attagacggc acgagagcta
     3781 aagaatttgc tgaagctgtg aagaatccat ccgaagcact agaaaaacaa aaagaaaaag
     3841 cttcagcagt caaagaagga ctaaaagatg cgtatggtga aggacaagtc aatgcagcag
     3901 tgaaggcaaa tacaacaagt gaaaaagaaa atcaagaagg tgcagcgatt ccttcagcca
     3961 aagatcacca agatgagtta cattctaacg aaaattcgat cggagaaaaa gattcagaag
     4021 aaggacaaca tggtcagtta ccaaaaaccg atccagaaca atatcgttat aaatctaaca
     4081 ttaattctga aaacgaaaaa cgcatttcag atttaccaaa acgaaatcaa gaagtacaaa
     4141 cggatgagga ttccatcgat ctgccaaatg ataatggcat cgaagttgga actgaaaaat
     4201 caacgaaaat tggtttgcct aagacacaaa atttaccaat gaaagatagt cgtttgaatc
     4261 aattcaaaga gaatgatcaa agctttgttc atcaatcacc tgcaggtact gttcaaatga
     4321 aatcaagtcg tttgcctgaa aatggaaatc tgacaactgt ttcttacaat ttagctgaaa
     4381 gtggtgaaac agagaatgcg tccgttcaaa atattgtgac aaaagaagct gcacctaagc
     4441 caatcaataa agttcataat ggatacagac cagcaaacgt agcatttact gcaccgacaa
     4501 aaataaatgt gacaagagaa actaaaaaca ggaacaatga attgaaagaa caactattga
     4561 aggatatcaa accataaaag gcctatttaa gggccttttt ctttataaaa caagtttaaa
     4621 atggaaagga gaaaattgtg gaggatattt taatagctac gtttataact gcactgggag
     4681 catttttaac gatatttgtg gtgactatta aaggatttaa agatacggat agtttacaaa
     4741 aagatcatca gtctatttta gatggtcaaa aaacacatac cgaaagcatt attgtacacc
     4801 tttcagaagg attcacttat ttgaaagatg ctatgcccac attcacagta aaattaacgg
     4861 aaatatttca ttctgttgag caaatatcgt cagaattgaa agagcaaaga caacaaaaag
     4921 atcgtcaact tggctcactt aattttaggc aagaaacgat acaagaatca ttggagaatg
     4981 taaatcgatt aaacgatgaa atgcaacgag ttcatttgga aaatgaagaa ttacgcgcac
     5041 aaaattatga tttaattcaa aagaaaagaa atctggaaca aaaaattgtt gaattaaaag
     5101 aagaaaatcg tcaattgaat tatcaattac gacgaggaaa aaatcgttct cgtgatttag
     5161 gaaatgaatt agagttgtaa tatttagaaa aacgctgtga gaaatcacag tgttttttat
     5221 ttctaaaaat acaaaatata aggggtatgg ttatattatg gataaataca ttcatatgaa
     5281 aaatcctcca agatttagtg gggaatggaa gtttttcgcc tttttctttt tagatctatt
     5341 aacgcttggt ggctgctttt tttgggcgaa aatagtcaat aatcttgtcc acttaccact
     5401 cttttttgca ctcttgaatt atggattatg gcttgcgata gcgttagtga ttgtttggcg
     5461 accctataat aaccctggtc aacgccaaat ttttgcgatt atcgaatcta ttttctttac
     5521 ggataagaac cactatcatg cgttagatcc taaccgaaat tatcatggta cgtataagga
     5581 ggatgaataa atgcgagtca aaggacaaaa ttcagttgat aagaaatcca ttttgcagtt
     5641 tggaaaacca gaagagcgct ctatcttgga cttatttcca tataaatcct gtacagaaga
     5701 gggagtctta attactagag aagatcattt tcagcgttat tatcgtgtcg tttccactga
     5761 cgtggaggga ttaaatgaac aagagaaact agaacgaatg aatcagttga ccaccgtcat
     5821 gcgtacgtat gtgccaacaa ttaagttagt tagtttgacc acagagacgg atctaacgga
     5881 acaaatcgtg caaaagcggc agttattaga taaaaatcgt atggaacagt cactaggaag
     5941 aaatctacag cgattaagaa aatatgaaaa gaaattagta gaggagatcc aggaattaaa
     6001 acgggcagaa caagaacgtc ctgacttaag cttctttttc gttattgaag gaaagacaat
     6061 caaggattta caaactagaa atcgtcagtt aatgcgatct agtggcattt tagggttaaa
     6121 accactctac aaaaaagaat tgattaagat tctttatcga atgaacaaca tgaatgacga
     6181 ataaaaaaga tggaggaaat gattcatgct agatgtgata aaaacagctt tttcaacgca
     6241 aagtaatcaa aaggtgattg atccattaaa acaacttggt aatcgtgaaa tagagatttt
     6301 aaaatcaaaa ggttatgatt tagatttctt aaaacaagtg atgccacaag gtgggttgaa
     6361 ttttgatgaa gaatatgtga cctatggaaa tggttattca cgatgtgtga cagtttatca
     6421 ttacacaaaa aatcctactc ttttttggtt ggccaatctc atgaacaatc ctcatacagt
     6481 ggccacacta gatgttagca cagatgaaaa gcaaaaagtg attgatcaaa tcaaccgcgc
     6541 attagatgaa ttaaatgatc gatcagataa tgaacgaaaa gcgactgacc gaaataaaag
     6601 tgcggaagaa tacctggaac tagcggaata tgcccgaaaa ttgacttcta atggggaaat
     6661 ttctaaacaa gtcaaagtga gagtacatgt ttacggaaat acgatccaag aagtggatga
     6721 acgggcgcag gaactgatcg aaaatctaaa agggatggat tatcgtgcgg tgacttatct
     6781 gcataaaacc aaacgagatt ggcaatcgat gtttaccatg ttcgatgaac aagctgaatg
     6841 gtttggaaat atcaaagggc aatcacttcc atcgttaaac attggcggag gattcccgtt
     6901 ccaccaccaa acgctaaaag atccaggagg cacacaatat ggggaaacca tgacaggcgg
     6961 cccatttgtc tggaatgcca cgtataaaaa tagtgtacgt ttatctacca ataccttgct
     7021 tctaggaatg atgggagctg gtaaatcgac cgttcttaaa atgatcgcgg aagcccattt
     7081 agctgcagga gactttattt ggggctttga aaagggaaaa gactttattc ctttcttaaa
     7141 ggaatataac ggtattatgg tgcgtttaga cggatctgac gggatgatca atcctttaga
     7201 gatctttgcc acacggacgt atgatgaagc atccagttta tatgacgatg gttcagtcaa
     7261 agatttgaag atcaatgaag cagcgtccta tcaaacgcac ttagacaaag tagtctatca
     7321 ggtacagtta gtgagtcctc agctaaaagg aacgatgaaa gcagaattca agacctactt
     7381 gaatcgtttt tatgaagagt atggaacagt accacgcggt tttacttcct caaattcgcg
     7441 ttcaaatact gatacgcaag tcacagggaa agatccagaa gcttatccga cgttcaaaga
     7501 atttttagcc tttttaggac aactggaact accaggggct tcccaagaga aaaagaatcg
     7561 gaaagaagaa atggagtcta tcgtggaatc cttatgtgag acttatggca tgatttttga
     7621 tggtcatagt acgattcgac accttgacca acaacagttg gtcttttttg acattgactc
     7681 tattacaggc ctagatcgag gggtacaaca atgtcagatg tacatggcca tgacgttgat
     7741 ttggaatcag gcattgattc aaggacgaag acaacgacgc ttaattaaag aaaaacaaat
     7801 ggaagaacaa gacttcaaac gcttcttggc gatgattgat gagtgccata atgcgattaa
     7861 tccagatttc attgaaacgg taaattatgt cacaaacttc caacgagaaa tgcgtaaagt
     7921 agaagcaatg actgttttag ctacacaatc cccacaagaa atggtgccag aaaatatgtc
     7981 ttctgataac ttctcgaaac tgaagaaagt ctttgaattt tcttctacga agatctttat
     8041 gcgtatggat gaatctattt tacgtcatat agaaggatta gttggtaaat caatgacgaa
     8101 ctcagaatta gaatccattc ctcaacaaaa tcaaggagac gctgtgatca attttggctc
     8161 aaaagaaacc attcgtgttc attttagtcc gaacaaacgt caattaaaat tgtttgatgg
     8221 aggaaaataa tggaaaacca aaatgaaagt ttaatcaaac agtatgttaa gcgtcgtgca
     8281 aagcggcgct tatttttgtg ggtgtttggt acttctgcag gtttgattac tatattgatc
     8341 actgtgtttg tgaccctttt tctaatactt gcggcaggtt ctattgataa ttctgattca
     8401 agtagtggtg gcgaagcttt tacaggagaa tattcggaag gcttacccat ttataaagaa
     8461 atcaaaggaa gaggcccttt ttctgatgag attgcgcaat atgctgtagg tgcagcagtg
     8521 aagtataaat tattaccaag tgtgatcctt tcgcaatacg gttatgaatc agcgtttggt
     8581 acgtcggcgt ccgcaagaaa tgatctcaat tattttggga ttacgtggtt tgatgggtgt
     8641 ctgtttccta aaggaactgc gcgtggaatt ggtggcattg aaggtggctg gtacatgaag
     8701 tttcccaact caaaagctgc cttctcctat tacggcttta tggtagccac acaatcaaat
     8761 tttaatgctt gtgtcggaaa taaaagccct ggcgcaagct tattgatctt aggacgagga
     8821 gggtatgccg ccgcaggaat tactgaagac agtgcctatt ataaaaattg tatgtctatt
     8881 attaatacaa ataaattgat ggagtacgat gagtttgcga ttaaacactg gagagaaggc
     8941 ggcggaactg gtggtacgat tacaggaagc tggggcaatc cattcccagg aagtagctta
     9001 gataaaaatt cgttcagtgg aggacaactt tttgggaaaa atccaggagg agaattccga
     9061 cctaacgggt tccatgatgg actagacttt ggttcagtcg atcatccagg aagtgagatt
     9121 catgcggttc atggcggaaa agtcgtgtac gttggcaatc ctggtatttc aggtttgggt
     9181 gcgtgtgtga tcgtgattaa ttacgatggc ttgaatatgg tgtatcaaga atttgcaaat
     9241 agtacaggaa attctcgagt gaaagtaggc gatcaagtga aagttggtca agtaattggg
     9301 atacgagata cagcacactt gcatttagga tttactcgaa tggattggcg tcaagcacaa
     9361 ggacatgcat tcattgatga tggtacatgg atcgatcc
//
LOCUS       DACDEG010000096         9359 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.100, whole genome shotgun sequence.
ACCESSION   DACDEG010000096 DACDEG010000000
VERSION     DACDEG010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.100"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            246..3014
                     /locus_tag="IV142_002325"
     CDS             246..3014
                     /locus_tag="IV142_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8485929.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            3096..3515
                     /locus_tag="IV142_002326"
     CDS             3096..3515
                     /locus_tag="IV142_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP8485930.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            3527..3997
                     /locus_tag="IV142_002327"
     CDS             3527..3997
                     /locus_tag="IV142_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8485931.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4014..4793
                     /locus_tag="IV142_002328"
     CDS             4014..4793
                     /locus_tag="IV142_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8485932.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            4780..5601
                     /locus_tag="IV142_002329"
     CDS             4780..5601
                     /locus_tag="IV142_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8485933.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            5618..6667
                     /locus_tag="IV142_002330"
     CDS             5618..6667
                     /locus_tag="IV142_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8485934.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            6680..7684
                     /locus_tag="IV142_002331"
     CDS             6680..7684
                     /locus_tag="IV142_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8485935.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8317..9336
                     /locus_tag="IV142_002332"
     CDS             8317..9336
                     /locus_tag="IV142_002332"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP8485936.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3113 a         1560 c         1970 g         2716 t
ORIGIN      
        1 ttttccagga agtatgtttc ctgtttttat atttatccga tttcattgaa tagtcatagc
       61 ctccttattc tttcatatgt tgaaattcca gtataaaaac tggaaggaac tgtacgtttt
      121 ttagaataaa aattataagg gataagtagg ctgctgtcta taataagata tatttttcaa
      181 ttctttatta aagattttat tgacaaaatg aatccgtttt ctatgatgga ggaggggtga
      241 ataagttgtt gaagaaggaa attttatgtt acttagagaa ccagacagcc tttttcgatt
      301 tagaacatat gaacgaggtc tttacagcac aaaggttagc tgatcatttt ggggtaaaaa
      361 gaaacacagt cagtcattat ttgaaccagt taaatgatga gcaactacta gtcaaaatca
      421 attctcgacc agtgatttat ttccacaaag aagcttttga aaaacaattt tttaagttac
      481 gcacgaattt ttatttttct atcgatgatc tcaaagagga acagcctttt ttcgcgcaac
      541 caaaagactt attctctttt atgatcggat acgacgccag tttgaaagaa agtatcgaac
      601 aaatcaaaac agctctttac tatccagatg gcggattacc acttttgatt acaggcgaaa
      661 gtggcacagg taaaagttat ttagttaatt tggtctatca gtattgtctg cttcatgatc
      721 tgctagaaga ttctgcacca ttcatcactt ttaattgtgc acaatatgca gataatcctg
      781 aacttttgac tagcaatttg tttggacatg ttaagggagc gtacactggt gcagaagaaa
      841 ataaaaaagg ggcttttgaa gcagcagatg gtgggatctt gtttttagac gaggtacatc
      901 gcttaacacc agaaggtcag gaaaaactgt tcacctatct tgatcaaggt gtgatttatc
      961 gtatgggaga acctaatttg tcacgccgga tcaaaacacg tctctttttt gctacgactg
     1021 aagagattac tagtcatttc ttaacaactt tcattcgaag aatacctatc cgaatcgaac
     1081 tacctgcact taatcagcgt agtcgtaatg aacggctaga actgatttac tctttttttc
     1141 tcgaagaaca aagaaaaatg gggagccctt tgtcagtcag tggaccagct ttgtcacttt
     1201 taacaagtca gaaattatcc ggaaacatag gagagctgaa aaatatcgtc aaagtttcag
     1261 cagcaaaagc atatgctgaa cagcgtgaac agcctgaaat ccatgtcacg atccatcatt
     1321 tgcaaaaaga actacttgcg cagcctatta ctaaaaatac agcacagcaa ggaatctata
     1381 ttacgcaaga ccagacctta gagcaattaa tggagaagca acagcccgaa cagcagcgaa
     1441 tcgttaaaag ttttgagcaa atcctaattg atttcaagaa aaataactgt ctgctatatt
     1501 cttgcgaagg aaaattaaag caagaagtga ttcaattatt tgattttttg ctgtttgaga
     1561 ccagtagaca agaaaaacac gagctgctgg tttattggac acaatctatc cgagagacct
     1621 ttcgacaaat ggaaactgcc tatcaaatca aattcgatgg aaacagtgtc tatgccttga
     1681 gttactatct ctatcagcga agcactatca aatggttacc ggaagaacat gatattatcc
     1741 aactaatcaa agaattagaa caacaagttt ctcagtctta tccatctagt tatcactatg
     1801 tccaacgaat tctggaatta agtaagatca aattagatat tgaagacaca agtatggatc
     1861 ggattttact gacgatctat ctaaaaaaag cgaaatggtc aaaagaaaaa cgactgccaa
     1921 aagcgatgat tgctgctcac ggttatgcaa ctgctagcag tatggcaaat gtagtaaatc
     1981 gtttattaca agaggatcta tttgaatcat ttgatatgcc actagatgtc acaccacagc
     2041 agattgcttc agaaatcctt gattattgcg aaaacagcga tgtttcaaat ggattgatca
     2101 tattagtaga catgggatct ctgaaagaaa tccatcagtt ttttaaaaaa cagttatccg
     2161 taccgctttt gattttgaat aatgtgacga cacctttagc aatcacagta ggggaatgcc
     2221 tgcaaaaaaa tgtatcatta gaagagatag cagaagagac tgttcgacag atccagccag
     2281 aatggcgact gctctatcca gaggaaaaca aacccaaagc actcattact acttgcttca
     2341 ctggaatcgg tacggcggtc cacttaagtg aactgctaga aaaaagctta cctacggctt
     2401 gtcagttgaa aatcatccca tatgaatatc agcaattaaa agataagaaa aacagtgacc
     2461 cattattttc catatacgaa gtcgtaggaa tgattggcac cacagatcct gatatcacca
     2521 atatcgacta tctttcatta gaagacctca tctcggggga aaagatgtct gtactagctg
     2581 aatggttgga cactactatg aatgcttcag agaaggaaat attcaatcag cgcatcattc
     2641 gtaatttttc attggaaaaa gtactggatt ccgtgactat tctagataca gagaaggtga
     2701 tgggagaaat cgatcatttt atgagagaat tagaggtgca gttgaatcga agcatcagca
     2761 atcccaaaaa attagcgttg tatgtccatg tgagctgtct cattgaacga ttgatccgac
     2821 aaatgccaat cgaaacctat caaggattag agaaatggaa gcagtgtcaa aaagaagggt
     2881 tatctgctat taaatctgcg tttagtgtca ttgaagagca ttatagtgtc atgatcccca
     2941 attctgagat tgcctatatc tatgatattt tgagtgaaga tacagagttt ctctcgatag
     3001 aagaagaatt ttgaacataa gcaagttgtt gccactaatt ttggcatgca acttgctttt
     3061 tttagtatgt gtaagtgaaa agaggagatg aaaaagtgga tagagaaatt atcttagctt
     3121 cccatggaaa atttgcttca gggatattag attcattaga gctgatctat gggaaaaatc
     3181 attctattac tgtattcgat tgctatactg atgagaattt tgatttagcg gaaacagtaa
     3241 ggcagctttt tatgaggtac aaagataaag aaatcattgt tctgacggat ctttttggcg
     3301 gaagtgtcaa caacgaattc ttacaatata tcaatcggga tcatgtctat ctcgttgccg
     3361 gattgaattt gccactattg attgagctag tatctcggtt ggacgctgaa gtagagacaa
     3421 ctgttttgat ccagcaggtg ctaactgatt caaaaaaaat gatccagttt tgtaacgagc
     3481 ggctcaatca gaaaatagaa gaagacgaat tttaggagga agaaaaatga ttgtattgac
     3541 aagagtcgac catcggttat tacacggaca agtagctttt tcatggacac aaacaatagg
     3601 cgcagactgt attttgattg ctaatgatga tgtacctacg aatgagatac gaaagacaac
     3661 gatcaaatta gccaagccac aaggagtcaa actagtgatc aaatcaattg atgactcgat
     3721 tgccgcgttg aaaagcgggg tgacagataa atataagtta ttcatcgtag tggaatcgat
     3781 cgaggatgca tataagctgg cagaagcgta tccagaaatc aaaaccatca acttaggagg
     3841 gatcaaggca aaacctggta ctcgcagcat cggaaaagcg gtaaatattt taccagaaga
     3901 ggaggaacgc ttgaaacgat taattgacaa acaaatagaa gtagaaatcc gtcaagtacc
     3961 agcagataaa aaagtggatg taagagaagt tttgtaagaa aggaagaacc tcaatgattg
     4021 tacaagcagt cttattagga attgttgcat ttgtcgcaca atcagaatat gcactaggga
     4081 catcgttgct ttctcgtccg attgtgacgg ggctgtttac aggaatcgtg ttaggtgatg
     4141 taaaaactgg gatcattatg ggcgcaactt tagagttagc atttatcgga tcgttctcag
     4201 taggtgcttc gattccacct gatgttgtga ccggtgggat tttagggacg gcttttgcta
     4261 ttacagctgg agccggtaca gaaaccgcgc ttttactagg tttgccgatt gctacgttga
     4321 cattgatatt gaaaaatatt tacttagggc ttttgattcc aattatgaac cataaagcag
     4381 acaattatgc agaagaagga aattataaag gagtcgagca tatgcacttg cttgccggta
     4441 tcggtctttc cttgatgctt ggtgttgtgg taatggtttc ttatatggtg ggaagcaata
     4501 cgatcggaaa tctattaaat atgattcctg acttcgttca aaaggggttg tcagtagcta
     4561 cagggttgat tccagctctt ggttttgcga tgcttgctcg tttgctgatc aacaaacaag
     4621 ttgcaccata ttttttctta ggttttgcta tagcttctta cttagagatt cctgtgaccg
     4681 gagtagctat ttttggtgcg attttagcag ttgtggtagt caatattatg aatgtccgtc
     4741 aactaccact tcaaacaact gcaggggagg tagaagaaga tgaagaattc tgagatcaaa
     4801 aacagcaaaa taacgaaaaa agagctaaat cacgtatttt ggcgttcttt tcaaatggag
     4861 ttttcttgga attatgaacg tcagatgaat atggcttatg tttatgcaat gattccgatt
     4921 ttgaaaaagt tatatcagtc gaaagaagaa atggcagcag ctttgaaacg gcatctagag
     4981 ttttttaaca cgaccccaca tatcgttaca ctgattttag gaatcacggc tgcaatggag
     5041 gaagaaaata aggatgatcc tgaatttgat gtgactgcga tcgatagtat caaaacatct
     5101 ttgatgggac ctcttgcagg gttaggagat tctttctttt ggggaacttt gcgattgatt
     5161 gccacaggtg tgggtacctc tttagccatg caaggaaata ttttaggccc tatcttgttc
     5221 attttgattt tcaatattcc gcacattctg tttcgctatc tagcaacagg ttggggatat
     5281 cgcttaggaa cgggtttttt gaaaaagatc caggcaaacg gtatgatgga aagtttgaca
     5341 ttaggcgcgt ctattattgg actgatggtc gttggagcaa tgactgccac gatgatcacc
     5401 atcaatattc ctttaaaaat cggttcagga gaaaatgctg tgactgttca aagtattttt
     5461 gatgatatcg ttccaaatat cttatgtctt ggagcatttg gcatagtgtt ttatctattg
     5521 aagaaagaag tgaaaccatt aacgatcttg atcggactag ctttctttgg aatttttggt
     5581 tcgtggatag ggatttttta aaaaaggagc agagaaaatg tttacgatgc aagattatat
     5641 ctatgaagaa aaggaagtac taagtacgat cttgaaaaag aatgattttt cgacaagaga
     5701 acatatgaaa aaaacagtga atttactgat cttagcaacg ggttcatctt ataacgcttg
     5761 tttagcagcg aagccagcat tggaatctta tggtgatctt actgtcgata ttcaagaacc
     5821 tttttatttt ttcaattatg gaaaactttc tccttcaatc gatactgtga ttgctgtgtc
     5881 acaaagcggg aaaagtgctt ctactgttga agcagtaaaa atgatacaaa aacaagggtt
     5941 acctgttgtt gctatcacaa atgacgtgca aagtccactt gctttagaag ctgcacaaat
     6001 tattgattta ggtgccgggg tagaatcagt aggcttcgtt acaaaaggct attctgcgac
     6061 tgttttacag ttactactct tagggatagg aattggaata agtaaaaata aaatcagcaa
     6121 aaaaatagaa caagactaca tgcaacagtt gcgcaaaata atcaaccatt taccagcgat
     6181 tattcaaaaa acggaagaat tttttgatgg atatcaaagt ttatttcgtt tagcacagcg
     6241 tttcgtcacc attggatatg gtccaaactg gggaacagct aaagaagcag agacgaaatt
     6301 gactgaaaca atccgcgttc cgtcacaagg atttgagtta gaagcttata tgcacggccc
     6361 atacctggag gcagatgctt cgcatttgct attctttatt gaaggggaaa gtgtaaataa
     6421 ggaacgttcg caaaagttgc aaagatatat gagtcggtac gttggagaga ctctcaccat
     6481 taccacaaaa aaagcaagaa acgagaaaac acttggatta gcaatagaat gtgatgaata
     6541 tctgtctgta ttagcgcttg tcgttccgtt tcaactattc gcttataaaa ccgcagtagc
     6601 taaaggtatt gacttgaaca aaaagatttt tgaagacttt gatacagtct tgaaaagtaa
     6661 attataagag gtggaaatca tgctgaaatt caatgaagaa gaacaaatca aagcaataaa
     6721 aggcgcacta gctttacgtc cgcaagtaga agaaatcatc gataaacttt atgcggaaaa
     6781 atttgacgct gtctattatc taggaatcgg tggaacgtat gcttcctcga tgcaagctgt
     6841 cacttatatg aatgggaaaa gcaacttgcc tgtctttgta cagcatgctg cagaatatta
     6901 cactactgga aacaaaagat taacaaaaga ttctatcgtt attttgtcct ctgttaccgg
     6961 tacaactcaa gaagtagtga aagcagtaga acaaatcaag aaagtaggag cgacactgat
     7021 tggatttatc gataaagcga acagtaagtt gtctcagcta tgcgattttg tggtcactta
     7081 tccagctcct ggaacagaac aaatcaaatt ttttatggca gcagatcgat tgatgtattt
     7141 gaatggagaa ttcgaagctt attcagaata ctatgaacag ctcgaacgtt atttaccaac
     7201 agggttagtg gaagcagaga aaaaagcaga tgcatttggc ttatcatttg cagaaaaaca
     7261 tcgacgtgat agtatgcatt attttattgg agcagggaat caatggggag ctgtttattc
     7321 atatgccatg tgttactggg aagaacaaag ctggcttcca tcgaaatcta tccatgctgc
     7381 cgagtttctt cacgggacgt tagagatcgt agaagaaaca accccagtca cattattttt
     7441 aggcgaagat gaacagagag tgttatctga acgtgtggct aaacttttgc caaaaatttg
     7501 cagcaattat acattgatag acacaaaaga ctatccagta gaaggtatca gtgaaaaata
     7561 tcgcggacgt gttttgtctt tcttattgat gcatgtagtg acacaacgca tcgatgctca
     7621 tgtggagcag ttgaactgcc atccattaga gatacgccgc tactatcgtc agtttgatta
     7681 ttaggataga ctggataaaa agaatataaa aaataaagag agcagcgata actgctccct
     7741 ttattttttt aaaatattgg catcaattac gttataaaac acattttttg cattatctac
     7801 agttcaactt ataatctgtt taatgggaca tgtgggtgcc aaccattttt tgttatgcaa
     7861 tagtactatt tagttgttgc atggatccgt agtaaaagtc caagtacaac gagttgcgcc
     7921 agtttacata gcgttttgta tccagaatgt tgactaaaac aaaagttcct atgtcaaccg
     7981 tcaacagaga caatctgtcc gttgcaggac ctgacagatt tagaaatgtt caatctgtct
     8041 cttatgtatt ttaaatcaat actcactagg agaagtttcc taacctccta atagtaatgc
     8101 tggtagtaac attgtcaaaa ctgcttcctc ttaggaacaa gcaaagcata acgagtgtaa
     8161 aaataaaatg tagactattg taacatatca ttgattattt caagtaaaaa cgataaaaga
     8221 agtaaacaaa cacttaaaaa caatggaaat tagtcccaac tttaagaaat gtatactata
     8281 atggaaatta tcaattattt atttggagga atatccatga aacaaaaaat cgcagttctt
     8341 ggtcctggat cttggggcac tgctttagca caaacattgg cagaaaacgg gcatgatgtc
     8401 cgtatttggg gaaatgtacc tgaacagatt gatgaaatca ataagtatca tacaaatcag
     8461 cattttttac ctgatttgac gatcccagaa tcgattatcg gatataaaga attggcagaa
     8521 gcagtggata acgcagatgc tattttattt gttgttccaa caaaagcgat tcgttcagtc
     8581 gcacaggaac ttattggtaa aatgaatact aagccaatca ttatacatgc aagtaaagga
     8641 ctagaacaag atacgcataa acgaatctca gaagttttgg ctgaagaaat tccagaagaa
     8701 aaaagacagg caattgttgt attatctggt cctagccatg ctgaggaagt ggcagtacat
     8761 gatatcacaa caattacagc tgcaagtatt gatcaaaaag cagcagccta tgtacaaaaa
     8821 ctgttcatga atgattattt caggatttat acaaataatg atgtgattgg cgtagaaact
     8881 ggtgcagcac tgaaaaatat cattgcaatt ggtgccggcg ctatccatgg attaggattc
     8941 ggagatgatg caaaagcagc aatcatgact cgaggattgg cagaaatcag ccgtcttggt
     9001 gtcgcgatgg gtgccaatcc tctgacattt atcggtttga gtggtgtggg ggacttgatc
     9061 gttacttgta caagtgttca ttctcgcaat tggcgtgccg gtaacttgtt aggacaggga
     9121 cataagctgg aagaagttct agaaaacatg ggaatggtcg ttgagggtgt ggcaacgaca
     9181 aaagcagcag tagagctagc tcaacagcta ggtgttgaga tgccaattac tcaaacaatc
     9241 tataatgttt tgtataatgg agaagatatc aaaaaagcag caaaagaaat catgttacgt
     9301 gacggaaaaa tggagaatga attttctctt cgttaaatag atgatcaata gttaatcgt
//
LOCUS       DACDEG010000097         9263 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.101, whole genome shotgun sequence.
ACCESSION   DACDEG010000097 DACDEG010000000
VERSION     DACDEG010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.101"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..662)
                     /locus_tag="IV142_002333"
     CDS             complement(<1..662)
                     /locus_tag="IV142_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8485937.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QL"
     gene            796..1083
                     /locus_tag="IV142_002334"
     CDS             796..1083
                     /locus_tag="IV142_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485938.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1499..3037
                     /locus_tag="IV142_002335"
     CDS             1499..3037
                     /locus_tag="IV142_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP8485939.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3034..3999
                     /locus_tag="IV142_002336"
     CDS             3034..3999
                     /locus_tag="IV142_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8485940.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            3986..5308
                     /locus_tag="IV142_002337"
     CDS             3986..5308
                     /locus_tag="IV142_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8485941.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5371..5475
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5549..6292
                     /locus_tag="IV142_002338"
     CDS             5549..6292
                     /locus_tag="IV142_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8485942.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6307..7299
                     /locus_tag="IV142_002339"
     CDS             6307..7299
                     /locus_tag="IV142_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8485943.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7311..8048
                     /locus_tag="IV142_002340"
     CDS             7311..8048
                     /locus_tag="IV142_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8485944.1"
                     /translation="MSIQISHLNVVIENLKVLEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8038..8760
                     /locus_tag="IV142_002341"
     CDS             8038..8760
                     /locus_tag="IV142_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP8485945.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8844..>9263)
                     /locus_tag="IV142_002342"
     CDS             complement(8844..>9263)
                     /locus_tag="IV142_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP8485946.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3027 a         1601 c         1960 g         2675 t
ORIGIN      
        1 tctaactgtt caagaggatc aagtggttca aatgtttctg tagtctgttt tgcttccaat
       61 tggggcaaaa cagatccaat ttccagatac ttcactttat tcaatgtata agatagaata
      121 gaacagctaa gtaatgtata cagaaaatgt gtgagatcca aataaagcaa ggagacaact
      181 aaaataccaa aagtaatcaa gaaaagactt ttgccaatct taatatgaaa atatttttca
      241 atggcttgat aaatgatatc tgttccgcct gtgcttccgc cgaatcgaaa gacgagaccg
      301 caaccaatgc cagaaagaat acctgtcatc aaactattga tcagtagact atctacagca
      361 aaatgtatat gaaggctctc ccataaatgt aaaaagaaaa ctagcgcaaa tgttccgacg
      421 atcgtccgga gaaaagcgtg ttttccaaaa agaaacaatg agataagcaa taaaggtaaa
      481 ttaaaaagca gtgaagtgac agaaggagcg aatccaaaaa gattatgcaa aagcaaagca
      541 ataccagtaa ccccaccatc tgccagctta gatggaatat tgattcctgt cacagctaat
      601 ccgtagattc ctgcgcctaa aatgattccg cacaattctg taaataaatt taattttttc
      661 atttctagcc tcttttcttt tgaaaatttt cagcaacttc tttattatac tgacttatac
      721 tcttacttca agaggaaaat gaaaaaaaat tatttttggc tgtttctttt tatttatcaa
      781 ataaggagtg gaaaaatgcg agcagcagac ttattgacaa ttcttacaaa atcacccaaa
      841 aaaacagagt atcaaggagt ttttttggct caagaacaaa aactggtgcc tgtgacaatg
      901 gtccagataa ataaagcaaa tcaatttatc tttttatttg aacacggcaa agcaccactt
      961 gctatgaaag acatctttct ttttttgatg aagaaccgtg agaaagctgt ttattatcag
     1021 tcaaaagaag aactcattcc tctttatgga gtgaaagaga tagaaaataa agtggtgata
     1081 taagtaaaaa taagaaaaca agacgtttat gatgtcaaaa caaccatttt ttacattttt
     1141 ggcaaaaaag tgtcatgata cagtataata ttcgttgaac aggccaataa aggtgttctc
     1201 ccccacgaga tttctacttt attggctgtt tctttttatt aaaataaaag ttgtgtgtgt
     1261 aaaaaagata tagctgattc caagtgacaa tttcgtatag atttttatgg tattcgtagt
     1321 gaaaaaacga gaaccaaaaa gttgatccga ctttgttgct tggattcttt ttttgtcagc
     1381 atatttagag gtattttttt cttttttttg ttcgtgctgt aaggttaagg caagacaagt
     1441 tagttgggaa aaaggaaaga aatgtttata cttaaagaat aacagaaagg aaggtcgcat
     1501 gaataatttt atacaaacat taagtgaaag aaaaggcgag ttgctggaag ctgcatttca
     1561 acatctgtcc atttctctca tttctttgct gattgcagca ctgattgcta ttcctttagc
     1621 gatttgggca gccaatcata aaaaaatggc agaagtctta ttgcagatca cgggtgtgct
     1681 ccaaacgatt ccttcacttg ccttattagg tttacttatc ccatttgtag ggatcggaac
     1741 tgttccagca ttgattgcac ttgttattta cgctttgctt ccgattttcc aaaacacgta
     1801 tattggtcta gctgaaattg acccgtctat tgaagaagca gcggtagcat ttggtatgtc
     1861 cagaatgaga cgcttgctga aagtagagct tcccatcgca ttacctgtca ttatctcagg
     1921 tatccgaact gccttagttt taatcatcgg tacggctact ttggcagcat tgatcggagc
     1981 aggtggatta gggacattta ttttgttagg tattgaccga aatacacctg ttttgacatt
     2041 gattggtgcg atcagttctg ccttgcttgc aattatattc agcggactga ttcgctggtt
     2101 gcagcataag aaacctcgat attccatcat tactttagca gcagtggtgt gtttatttgg
     2161 cggcgtttct ttatatcagt ctgccttgtt taaaaatgaa accatcacga ttgctggaaa
     2221 attaggagcg gaacctgaaa tcctgatcca aatgtataaa gaattgattg aagacaacac
     2281 aaaaacagaa gttgaattaa aaccgaattt tggcaagacg agttttttgt tcaatgcact
     2341 ggatcaaggt caaatcgata tctatcctga gttcacaggt actgtcctag aaagcttggt
     2401 gaaggtacct gaagcaatga aaagtgctga tttgacaaaa gaaaaaacat atgacgaagc
     2461 agaagaatta ttgaataagc aattcgatat gacattatta aaaccaatgg cttaccaaaa
     2521 tacgtatgcc ttggcagtca agagatcttt tgcgaacgaa catcaactta aaaagatttc
     2581 cgatttggca gctatctctg atcagatcaa agcgggattc acactggaat ttattgatcg
     2641 ttcagatgga tataaaggaa tccaagaagc atatggcttg aatttttcaa gtgtacaaag
     2701 catggagcca agtttgcgtt accaagcaat caataatgga gatgtcaata ttgtggatgc
     2761 ctattccaca gatagccagc tcaaggagta cgatctagtg actctagaag atgatcgagg
     2821 gttattccca gcttatcaag gcgcaccgct gatgaaaaaa gattttgcta aagaacatcc
     2881 tgaaatcgtt aaagcactga ataaattaag cggaaaaata acagaggatc aaatgattga
     2941 tatgaattac caagtcaatg tcgaaaagaa aaagccgaca gaagtagcac gaatcttcct
     3001 gaaaaatgaa ggattagtaa aggaggaaga tcagtgacta aattaatcga gttcaaacat
     3061 gtgcaaaaaa agtatgatgg aaagtatgtc atcgatgacc taaatctggc aatcaaaaaa
     3121 ggagaaatct ttgttttggt tggtccttcc ggtagtggaa aaacaactac tttgaaaatg
     3181 atcaatggat tatctaaacc atcagcagga gacatctact tcaaaggaaa atcgttaaat
     3241 gaatataatc tgcagaaaat gcggtggaat atgggatatg tgctgcagca aatcgctttg
     3301 ttcccgacca tgacagtcaa gcaaaatatt gaagtcatcc ctgaaatgtt aggttgggaa
     3361 aagcagaaaa gagcagaccg tgtagatgaa cttcttcaaa aagttggctt atctcctgat
     3421 atttatcgag atcgtatgcc tagagagttg tctggaggag aacagcaacg gatcgggatc
     3481 atccgagcta ttgctgcaag cccagatgtg attttgatgg acgagccatt tagcgcatta
     3541 gatccgattt caagaaactc attacaagaa ttggttttgt ccttacacga agaattgggc
     3601 acgaccatcg ttttcgttac gcacaatatg gaagaagcaa tcaagctagg tgatcgtatt
     3661 gcatttatga aggatggaga aattatccaa tgtgacacac cagagcagtt attgatgaat
     3721 cctaaaaacg actacgttcg gcatttcttt gatgaaccga aacagacgaa agagtggcga
     3781 gtagaagatt tagtcgtcaa tggatatttc ttaaatgaaa taccagaaaa tgctcgtcaa
     3841 caagtttgct ttgatacgcc tatgaaagaa gtatattcgc ttctttcggt gtatccaagc
     3901 attgtgatag tagaaaagca aagaagtatt gggtcgctga caagtaaaga aatctttgct
     3961 tttttaagtc gagaggagga aggaaatgaa aacatatgat gcaatcatca tcggttcagg
     4021 tgtgagcggc ttaagtgctg cttatggatt gaaagaagca ggtaaaacag tactagtggt
     4081 cgaagaagat ctatggggcg ggacttgtcc taaccgtgga tgtgatccta aaaaagtctt
     4141 gttaagtgca gtagaggcac gtaatcgggt aaaacaattg agtggaaaag gttttaacga
     4201 gatcccaact gccaactggg aagaacttca aaaattcaaa cgaacattta cggatccagt
     4261 cccagaaagc cgaaaaaaac aattagcaga agctgaaatc gatcatcttt caggaaccgc
     4321 tcgttttttg gatgattcat ctattgaagt caacgaagaa gtcttccatg cggattactt
     4381 agttttggca accgggcaaa gacctacgat cttaccagtg gagggaaagg aatacttgaa
     4441 aactagtgca gactttttat cattgcctgt actgcctaaa gaaatcattt ttatcggtgg
     4501 tggttatatt gcttttgagc tagctacgat tgccaatgct gcagggagca aagtaaccat
     4561 tgtccatcat aatcaacggc cattaaaaga atttgaggcg tctttggtag aggaagccgt
     4621 ccatcaaatg gaagcatctg gcattcagtt tgcttttggt gttgaaaccc aaaagatcat
     4681 ttcagaaggt actcgctatc gtttagtcgg aaaagagacg gaattagtag cagatatgat
     4741 tttttgtgcg actgggagac agccgaatac agaatcttta gcacttgagc aagcgaatat
     4801 cgtctttgat aaacacggaa ttgcagtcaa tgattatctg caaacgagca atccaaaaat
     4861 ttttgcgtgt ggagatattg tttcaaggaa aaccccaaaa ttgacaccag tcgccacttt
     4921 tgaaggaaat tatgtagcta agcggataac ggatgcaaca agcgaaccta tcaaatatcc
     4981 gattatacct acgatcgtct acgccagtcc taaacttgct gaagtaggag ttacaaaaag
     5041 tcacgcctct tcgtcagatc aagtggtaga gatggatctt accagttggt tcacctatca
     5101 tcgtgtcaat gaaccagttg ctaaggctga attgaccttt gaccagcaaa attatttgat
     5161 tggtgctgca gtgattagcg agcaagcaga cgaattgatc gatgatttga cactcgtcat
     5221 caatcaaaaa ttgactaaaa aagagttgga tagctatatc atggggtatc cgacattggc
     5281 aagtgatctc tcctatttat tgaaataatc agcatgataa ctaaagcgct tgttgctttt
     5341 tccttcttgt gctacacttt aaaaaatgaa tacctgtttc tggggatgtc gtagacgaca
     5401 tgagagtaag ttgtgaactt agacccattg aacctgtcag ttaatactgg tgaagggaga
     5461 aacatagcag acgcttgaag cgggttacta tgtcgtgccc atttcaagcg ttttttgtta
     5521 ttcatattag aagaaaggag gtaacaaaat gaagaaaaat ctttatccag cagtcagtat
     5581 ggcattcatt ttaattattt ggcagattgt tgtcatagtt tggaaaatac ctgcctttat
     5641 tttacccgga cctgctgctg tattttctgc attagcggca gatgcacaag tattactttc
     5701 ccattctgtc acgacattaa atgaagcagt gcttggtttg gtaatagcta gcgttctttc
     5761 ttttattact gccttggcaa tggattattg gaagttttta gaaatgtctt tttatccgtt
     5821 actagtgatt tcacaaatgt tgccaatcat ggtgttaggg ccattactta ccctatggtt
     5881 tggtttcggt atgcttccaa aagtgatcct tgttgtcttg ataagctatt ttccgatcgt
     5941 agtttctttt tcggatacat tgaggaaagt atcaaaagag caaatcatct ttttgaaaac
     6001 gatgggggca gatacaggga agatttatcg aatatataaa ataccagcgg gtttatccgg
     6061 cttcttttcc ggtttaaaag tggctgctac ttattgtgtc agcggagcag tcgttggaga
     6121 atggctaagc gctcaagcag gcttagggta ttacatgata cgcgtaaaaa acagctacca
     6181 gatcgataaa gtttttgctg cgatcatttg tgtcattcta ttgagtttat tgctgaatgg
     6241 cgcttgttca ttattgaaaa aagggtatta tcgtatgctt tatatttttt aagagaaggg
     6301 gatcaaatga aaaaattatg gcttttatta ccgctactgt tgcttttatc cgcttgtggc
     6361 actgcaaaag aaacaagtag caaacaagaa ataaaggatt tgaaaaaagt gacacttgtt
     6421 ttggattatg taccgaatac taatcatact ggcatctacc tagcaaaaga aaaaggctat
     6481 tataaagagg ctggtttaaa tgtccaaatc atcgaaccag gagataattc gacaagtatt
     6541 ggtctagtag gtgcggacaa agcacaattt ggtgtaagtt atcaagaaga tgtcacttat
     6601 gctcacgcag atggacagaa tataccagtc aaagcaattg ctacagtgat caaacataat
     6661 acttctggat tcgccaccct atcagacagt aacatccatt caccaaaaga ttttgaagga
     6721 aaaacatatg caggttggca aagtccaagt gaagaagctg tcttaaaagc tgtgatggaa
     6781 aaagacggcg gggacttttc aaaactgaca atggtaggaa gcaatggaga agggccagaa
     6841 agcttaggaa aatcctcaga tatccaatgg tactttgaag gttgggatat gatcaaagca
     6901 aaagaagcgg gtattgaact aaactatatc ccattgaaag aattagatga acggttagat
     6961 tactatactc cggtaattat tacgaatgat caactgatca aaagtgatcc agaattggta
     7021 cagtctttta tggatgccac gaaaaaaggc tatcaggaag ctattaagga tccaaatgac
     7081 agtgctaaac tgttgcaaaa atatgcaaaa gaaaatgatc ggacgttttt agaagaatca
     7141 caagcattct tgtcaaaaaa ttatacggat gatcctgaga attggggatt gatggaagaa
     7201 aaagtttggc gcaattatac agcttttatg caggaaaacg gattgatcaa gcaagatgta
     7261 ccttcgcaag aactattcac taatcagttt ataaaatagg agtgatagat atgtcaatcc
     7321 aaatcagcca cttgaatgta gtcattgaaa acctgaaagt attagaagac atccatttca
     7381 ctatccctaa agataccttt acatcgattg tcgcgccaag cggagccggt aagtcaacat
     7441 tgctgaaaac tttaacagga gtccagccta tgacttctgg aacgatcaaa gtagacgatc
     7501 aaaaagtaac tggtctccat acagcattta gttatatgcc gcaagaagat atgttacttc
     7561 cttggctgag cgtctatcaa aacgttacgt tatatcaaaa aatcaatcat ctaacaatca
     7621 atgaagaaca agtaagggat tatctggata tctttgggtt aaacggctat gaacattttt
     7681 tgccggaaca attatctggc gggatgaagc agcgaacggc cttgttacga acaatcatga
     7741 atccttcttc ttacttgctt cttgatgaac catttggagc gttagatgct atgaccagag
     7801 ggcagatgca agactggatg ttgaagctgc ctaaaaaagc aaagcggact actttacttg
     7861 tgacgcatga tatcgaagaa gcaatttatt tatcagacag gattcttgtg ctttccgccc
     7921 gtccggcaca tgtgattgct gagattcaag tgcctgaaaa acgacggtca cgtgaatggc
     7981 tactgcagca atcagagttg aagcaaacga tttatcagtt attggcaggt gaattagatg
     8041 ttaagtgatg ctcattgtca tttagatggg agtcagagtt tagcactcct tcagcaggaa
     8101 cacacgattt tgacgatcat caattgtgat tcgccagaag aatggaagga aaacagacag
     8161 cttgcagcaa gtaagactca agccttaagt tacggtatcc atccttggaa agcagatagc
     8221 tatacgtttg aacaagttga gccttttttg aaaaaagcaa ggatcatcgg tgaaataggg
     8281 ttagataata tttggacgaa tgtaccgatg actacacaaa aaaaagtatt tgagcgacag
     8341 cttgcttttg cagccataaa tgagaaacct gttgtactgc acacaaaagg atgcgaaaaa
     8401 gagatcttaa attatatcca agcttaccct aatcactatt tgatccattg gtattcttct
     8461 ttggactatc aacaagacta tattgattta ggctgttatt tcagcattgg tcttgatttg
     8521 aaaaagaatc cagcagtctg gcaactagct aaagcagtcc caatcgacca cttacttatt
     8581 gaaacggatg gactacatgc agcagagtgg gctttagggg ggattttttc agaaaaagat
     8641 tatgtctcac tgcttgaaga acagttaaag acaatcgctg agatcaaaga gctatcagtt
     8701 gaagaagtta aaaaaaatac ttatcaaaac ttggaacgtt tcattagatt aggtaaataa
     8761 aaaagagatg tgacacaact agtgtcgcac ctctttttgg ctttctttgt ataaaagtaa
     8821 actgtttgat taataaatga atcttagtac caaccgtttg cttcccagaa tgctttcgcc
     8881 gcttcccaag aaccataacg tgaagtaaca tattgttctg ctactctttc ttggtttgca
     8941 gcagagtagt caccatttaa gtaagaagaa tctaattggt aacgtccgat gtaacgtccg
     9001 ttcgttgctg tgtaagaacc gcttgattct ttttgtgcga tccattcttt tgcagagctt
     9061 gtgcttgctg gagtagcagc agtttctgtt acagcaggag caggttgtgt ttcagcaaca
     9121 ggcgctgctt gtttttccgt ttgaaccgtt tcttgtacaa caggttgttc agcttggact
     9181 ggtgcttctt gaacggctgc ttgaggttca gcagctgctt gtgcagagcc ttctgttggg
     9241 attgttaatt gttgtcctga gta
//
LOCUS       DACDEG010000098         8954 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.102, whole genome shotgun sequence.
ACCESSION   DACDEG010000098 DACDEG010000000
VERSION     DACDEG010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.102"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(1..199)
                     /locus_tag="IV142_002343"
                     /pseudo
     CDS             complement(1..199)
                     /locus_tag="IV142_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            440..622
                     /locus_tag="IV142_002344"
     CDS             440..622
                     /locus_tag="IV142_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485947.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            606..791
                     /locus_tag="IV142_002345"
     CDS             606..791
                     /locus_tag="IV142_002345"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAP8485948.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            1034..2554
                     /locus_tag="IV142_002346"
     CDS             1034..2554
                     /locus_tag="IV142_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP8485949.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(2741..3313)
                     /locus_tag="IV142_002347"
     CDS             complement(2741..3313)
                     /locus_tag="IV142_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8485950.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(3320..3982)
                     /gene="rpe"
                     /locus_tag="IV142_002348"
     CDS             complement(3320..3982)
                     /gene="rpe"
                     /locus_tag="IV142_002348"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP8485951.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            complement(3998..4513)
                     /locus_tag="IV142_002349"
     CDS             complement(3998..4513)
                     /locus_tag="IV142_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAP8485952.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            complement(4515..4799)
                     /locus_tag="IV142_002350"
     CDS             complement(4515..4799)
                     /locus_tag="IV142_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8485953.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            complement(4833..6161)
                     /locus_tag="IV142_002351"
     CDS             complement(4833..6161)
                     /locus_tag="IV142_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP8485954.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            complement(6175..6630)
                     /locus_tag="IV142_002352"
     CDS             complement(6175..6630)
                     /locus_tag="IV142_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8485955.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            complement(6633..8579)
                     /locus_tag="IV142_002353"
     CDS             complement(6633..8579)
                     /locus_tag="IV142_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8485956.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
BASE COUNT         2965 a         1663 c         1417 g         2909 t
ORIGIN      
        1 tcagcatttg ttcgagctcg ccttcggcct gttggaaacc tttatgactg cgacctagga
       61 atttttggtt gtaagtatca aaagacgaga ctaagaaacg ttccatcgac tcttcatttt
      121 ggaactgttc cttgcggtgg ctgtatttct tgatttgttt attgaaagat tcgattaaat
      181 tagttgagta aatgcttcgt acgaacatag ttgatttatt caccatactt cccattacat
      241 cgtaaaacgc tatatttaag atttcaaaca actatattaa taggaagaaa acttcatttt
      301 tcctcttagt tctataaaaa gtgtggctaa tttgaggcaa aatattaaat taaatgtaag
      361 cgtgtcatag gatagtatct cgtacgccga ttatctttct tctataatta aactatcaaa
      421 tatagaagga gaaaaacgaa tgaataaaaa agatattgta ccagttcaat tttcaagtgc
      481 ggctcaaaaa tcgcaaattg acaccaagcc aaaactagct tgcagaatca aatcggaaga
      541 taaggaaata tttatttata atggaatgga taaatatctt ttgtatacct tgttaaaaga
      601 gttgaatgcc aatgctcatt gatgcaactg cattgaaaca tatttttatc gtttgtggaa
      661 aaacagatct tcgaaaagga attgatggac tagcagcttt cgttatagaa gaatatgacc
      721 ttgatgttta cgatcaagcg cttttcttat tttgcggtac gaagaaagat cgcttcaagg
      781 ctctttacta ggatcaaaat ggatttgtat taatgtacaa aagatttgaa agtgggtacc
      841 ttcaatggcc gcgttccaga aaagaagtct tactgactgg cctttcaatc gaagaaaaat
      901 gtacgctaaa agaaacgaaa aaaggtgcga tatattagac gaaaaccttg ttaaatcaag
      961 gattttgtct gtttttcgtt gtcataaaat ggtataatag atgtaagaaa atcgctagaa
     1021 tggtggtgta aaaatgacaa aaatggaatc tgaacttctt gtacaaatca agcaattaac
     1081 acagttgctt gaaatccagc aacaggaaaa tactcttctt agagaacaaa ttgctgaaat
     1141 gaatcgacgc ttgtttggac gaaaaaaaga aacaccaccc gtagatgggc aaatcgattt
     1201 attagacgac tcaactttta atgaaccaga gcacactgga caagaaagcc aggaacccat
     1261 tacggtatct tcttttaaac gcagaaaacg caagggactc aaagcacttt cattagaagg
     1321 tctgcctgaa gttcctattc attatgaatt aaaaggtgcc gattgtttgt gtgaagactg
     1381 tggtcatact ttacatgata tcggtgcgac aaggcttaga gaagagccat tgtatgttcc
     1441 tgcccatata gaaaaacaag tcatctacca acatgcctat cattgcagag aatgtgaagc
     1501 attaggagaa acaaaaatca aaaaagcgcc tgttcctaaa ccgttgatca ataatagtct
     1561 aggttcctct tcaattgtga cacagagtat ttgggaaaaa tttgttaaaa aggtgccggc
     1621 atatcgacaa gaaaaagaat ggcatgcact tggtttccct cttgatcgta aaaaaatcac
     1681 taattggcat atcaaggtaa gtgaaatggt tttagagcca atctacaatt gccttcatca
     1741 agaattaagc aatcaggagg tcttacacgc ggatgagaca agctatcggg tattgaatac
     1801 tcataaagaa aagacctttt actggttata tgcttcaggt aaaagcgaag aaaaacaagt
     1861 tgtgctctac gagcacgcaa attcgcgttc cgttgatgag ccaaaaagtt ttttagaatc
     1921 atttagcggt ttcttgcata ctgatggtta cgcggcctat ggaaagctgc cggtaaacaa
     1981 tgtttattgt tgggcgcatg ttcgcagaaa gttttttgaa gcattgggta aacaaccgtc
     2041 cccaaaaagt catgccaaaa taggattaga cttctgtgac aagatgtttc gaatcgaacg
     2101 gacctttaat aagcttagcg cagaagaaag gtttgtagcg aggaaggaaa cgttgcagcc
     2161 tgtgatgact gaattttttc attggtgtga gagttttgct tatttgccaa aatcgcagct
     2221 tggaaaggcg gttcaatacg cagtcaatca taaagacgga cttcagattg tgttgcttga
     2281 tggacgcttg gaactttcaa ataaccgagc agagcgtgcc atcaaagagt tagtgatcgg
     2341 tcgaaaaaac tggctatttt ctaaaagctt aaaaggcgct agatcaaatg gaattatatt
     2401 gagtattatc cagacagctg ttgcgaatgg gttaaacatc cgcaaatatt taaatcatct
     2461 ttttacagag attcccaatc tgtcttcgat gacacctgaa gcgctacgcg cttatttgcc
     2521 atggaatcag cagatacaag aaatctgtaa ataatataag ccacaaataa cctgatttga
     2581 gtgtatcaaa tcgggctatt tgtgtttagg taccattcta tgacacgctt acgctcaaaa
     2641 ctactgaatt attaagttat gtctaatcaa ataaaaaaac cattctcata tttgctgaat
     2701 aattagcatt tagagaatgg ttttttatct ttaaaatcta ctactcaaaa tctgcatgtc
     2761 gagcaaacat cgtttctgag ctttcacctg ttttatccat taaagataac actaaactat
     2821 caaaggtgag aaatgctaat tgttcaaaac ttgaacccat aggttgtgaa aaagtatctt
     2881 cttctcgttg attttcatct ttggtaatac ctggtaatac aagaacagaa tctgctaatt
     2941 gaccaatcga cgagtctttt ttcatggtaa ctaaagcaat gcttacacca ctttgatttg
     3001 ctttggccgc taaatttttt aaactacctg tttcacctga gccagaacag ataattaata
     3061 aatctccagg ttgcgaatgt ggtgaactaa tttcaccgac aaggctgact gaaaaaccta
     3121 aatgcattaa gcgattcgta aatgcttgca tggcaattcc agagcgtcct gcaccagcta
     3181 gaaagatgtg tttagcttga acaattttct cactaaattg ttctaactct acagaattaa
     3241 tacgttttga gttattttgt aattctaaga taatcgcttc taaatagttt ttattttggt
     3301 ctttattggt catgatcttt tacttcactt cctctaatga ttgatttagt ttatcaattt
     3361 gttcttgcgg ggtatttttc ccattgaaga tgaaaccgcc tgatacaaaa atatccgccc
     3421 ctgctttaga acaaacttta atcgtttgat tatcaatctt gccatctact tcaatatcat
     3481 acgtataatc attttgtttg cgtattacat ctaactgttc gattttaact actgtctctt
     3541 gaataaaatg cttattctct gttcctggat tgattgtcat tactaatact tgatcgacta
     3601 aagataatac tggttcaata aaactgactg gtgttgcagg attaaaagta atccctactt
     3661 ttgctcctgc ttccttgact aactgaatga ccttcattgc gtgtttggta gcttcaatgt
     3721 ggaaggtaac aatatctgct cctgcttcta caaatcttgt gacaattttt tctggtgtat
     3781 caatcatcaa atgaacatca aacacaacgt ctgttagtgg acgaatatct ttaataacac
     3841 ttggtccaaa agccatatct ggtacaaaac agccatccat tacatcaata tgcaaataat
     3901 caacattctt ttcttttaag atttcaaata actctttaac ttctgcgata ggtgcattta
     3961 acaatgatgg agcaattgtc attctctatt cccccgttta ttctcccata atcatgagat
     4021 tgattgttct agttcgctct cttagttgat cccaatcgac aaaatagaca cggcccattt
     4081 ctcctaataa aagtttacct tcttgaacaa ttaaagtcat tgagttggtt ccataaatag
     4141 atgaacgtaa atgtgcatcg gtatttagca tgacccattt ttctgcaggg taattgggat
     4201 cagataatct tgttccaaaa tcaatgtgtt ttggccccgg actgttgtat tgattttcac
     4261 tcgtcatttt tggaataatc gtttccatta cattgttgat gtctacatgt aaataatcat
     4321 caccaaaaaa gtttgtgtca tgcatcgttt cttcaaaaaa gagtgagcaa gttgtatgcg
     4381 ggctcgaaat tactaacatt ccattctcaa tacctgctgt atcaactgta tctaaaagtt
     4441 cttttcgaat atcatgatac gaaggacgcc ctgaaactgt ttcaatagtc aaagtgtcat
     4501 gatgaaaacg catattaaac acctaatctt tcttttacat ctgcataaat ggcatctgta
     4561 ccaatacctg ttaataagcc caagccattc gttactttag ctttaattac atcagggaca
     4621 attgttgtac ttactacagc atcatattca tcaattttat ttagctcatc agcaatttta
     4681 gattgataaa ttttcacttg atctgttaat ccttcttctt ccaaccattt tgataatttt
     4741 gctactgcaa ttgtagaagt agcatgacct gctccacaaa taactaataa ttttttcata
     4801 ttgatctctt ctttcttatt ttaattttat gattaagctt tcttcacttt gttcatgtag
     4861 atagaagcac ctactaaaac aactcctaat ccacctattc caattacacc aaagtaagat
     4921 gctaataaaa caaacaaccc tactggccat aatccacata acaacatagt tactaatcct
     4981 gctgtcccca catcgaaact tgctaatgta aacacatcag tgataattgg tgccaaccat
     5041 gttgccatat ataatgttgt aacaacatac aacgaacatc ctactaatga tctcacaata
     5101 tttcctttaa aataaggaat cattaaacag atggcgaaag caaagaatgc taaatctcca
     5161 aaaggtaata ctttatttcc tggaagaatt agtgctagca ataatgagat tggtacaagc
     5221 aatagagaag ttgacataat agttggatga cccacagtta acgctgcatc cattccaatg
     5281 tttacttggc gaccatttaa ctttttattc gtaaattctt tcgccgcttc tgcaattggc
     5341 attaaacctt ccataaacat tgctaccatt ttaggcataa tcttcatcac tgccgcagtc
     5401 ataattccta agtttccaat tcctgctacg tcatagcctg ccataatacc cactacaata
     5461 ccgataatta ggccaataac aactgtgtca cctaaaacac cgaaacgttt tgttaatgtt
     5521 tctgcatctg catctatttt attaaatcca ggaatacgat caaaaaccca atttactggt
     5581 ttggcaaata gtactcctga taatcccatc atatgtgtga tagagattcc tggatagccg
     5641 aaaaattcgc taacagtttt ttgtgtacga tctgctaaga accattgtaa aattactgcg
     5701 acaatcgcgc caattaagcc aaaccaaaag tttcccgtaa cagattgagt aatcatacct
     5761 aggaaaattg cgaaccaaaa gttccaaata tcaacattca atgtttttgt ccaacccaaa
     5821 accacaaata tgaagttaag aattaatgaa attggaatca ttaaaattcc caatgatgta
     5881 gagtaagcca aaggtccacc aacacctacg ccaatatcta ggatattcag gccaccaccg
     5941 tattgttccc ccatttgttg aattgctgtt cctaaaccat ctgataataa acttaaaact
     6001 aatcctaaac caatagaacc gataccaaca gtaacgccgg aaacaaatgc tttaccaggt
     6061 tttaatccaa ataatatccc gattacaaaa ataatcagtg gtaaaaagac gtttgaccct
     6121 aatccaataa accattcaaa ggcatttaat acaatatcca tctctctatt cctcctaata
     6181 aactcctgca gaatgtacta actcaataaa tgcttctttc gtggtacatg caagtaattc
     6241 atttactttt tcttcatttt gaaataggct aactaagttt tgtagtgtat caatttgttc
     6301 atgaggttgt gacattgcca acataaagac taaacgtaca ggtatttcat gagatttatc
     6361 tgtcatatct gaaaatacca gtggttcctt taatgaggca aaacagattt gagagctatt
     6421 tacatgtgcg gaatctgtat gtgggatagc aaacccaatt tgatttactt ctaatccagt
     6481 tggaaattct tgttcacggt tggtgatacc ttctaagaaa ctatctttca cgcaaccagc
     6541 gttttttaat tgttcagaca ttacttgaaa caattctttt tgatctttca cttctaattg
     6601 aaataaacat aattctttat ctagatacat ttttattctc ctttttgttg aaaaattctt
     6661 agtaattttt cgctatcatg aacttgtaac aagcgatcga ccatttcttt ttgcccaata
     6721 aattggtaaa gttcttcata tactcctcta atctcagata catcttcttc agctaaacta
     6781 accataatga ctaatttgac ttttcgatcg ccccaattaa tcgcattttt taatgctaca
     6841 ataaaaatgt tggattcaat gaccattgat ggattcgcat gcggtaaagc aacaccactt
     6901 tctaaattag tattccccat tttctctcta gagaagatag aagcacgaaa ctcttcagta
     6961 actaatgaat tggcttctag ttcggaaatc atcaaatcca aacattcttc tttgctttcc
     7021 acctgtactc ctgtttttac taaagatgat tgaacaaatt tagatatggt gggaacttta
     7081 accgctttag gaatcagttc attttgaata aatcgcgaag gttcttctat taaatattta
     7141 gtataagctt ccataatatt catataatct gttttactta ccaatggaga tacccttact
     7201 aagggtatac cacctgcatc aaaggaaaga gaggaaataa tcaaatcaac ttccccaatt
     7261 tcagctaaat gaaacttatc taaagttgat atttctacat tatcttttgc tggtaaaaac
     7321 cgtcgaactt tattgtaaat taattgcgca gaagaaatac cgtattggca aataatgatg
     7381 atattatttg ctttggactg atcttcaata gcaatctgaa aatgaatcaa aattaaggaa
     7441 acttcatcgt ccgttaaagt cacatcaaat tcttcttcaa ggatggacat ggcgtaccat
     7501 actatgctaa agagttctga atattgagat ttgatatttt ctaataacgg attttgaatt
     7561 ttaatacctt tttttaaacg aaggaccatt gctggcacat gatacaataa ggattgatat
     7621 aatcgatcat tttgagtcaa atcaattttt tcaatctcac tcattctttc aatcaattta
     7681 tgaacgctca tagcgtactc ttcattgctg gtttttaaat tatttgtcac acgatgtgca
     7741 aatagttgac gacatagata ttctttatct gcgtcataaa atggaagatt caattttgcc
     7801 ttcaattctt caattaaacc atttgccaca atgtacgttt ccatatagcg aatgctattg
     7861 aagaggaatt cttcttcaac agttaaatga tgacctttca gtaaacggtt acattggata
     7921 actaaagtag aaatcaatga ccgaacatag taatcagaaa ccttttcagt cagttcactg
     7981 taatcattga atagcaaatc atgaatgaca ctaataactt ttgaatcaaa caacatattt
     8041 aaaacttgaa aaaatgtttt tgattccatg tcattcgcat aatagaatac aagttgcttg
     8101 attgctcgtt gaattgcttg ttcattacca gtaaaactaa ttccttctgc agatgactgc
     8161 aacttaactt ctccactgcc aataatttta ttgataattc tcaggtcatt gtataacgat
     8221 gttttactaa ccataaaact ttgagacaaa tgagttaaag aaacaggctt atcaccaaac
     8281 aaaacttctt taatgatctc gacacgtctt ttttctggag aataaatatc tttttcaaga
     8341 taattttcta ccgatgaaat caaatgttca acgtttttcc tatcacctat gagtttgata
     8401 cccaaagaag gtaatcgctc aattgttact tggtaattaa ctacataatc ttccaacaat
     8461 tcaatatctt gataaatggt cttggtcgac actgccaatt tatcactaaa aaaagaagcg
     8521 ggtttaaagt cttcttgtcg acttaataat tttaataaat ttacatgtct ttcaatcaaa
     8581 cttgttcacc tcttaacgct agcatttgtt acctcttttc attatttatt ctagcactgg
     8641 aacccccttc cctcaattac atctatgtca ataatacttg tggaagtttg ttgtaaagga
     8701 aatccaaata atcttaaaaa atgagaaaca aaaaaggaga aatccttaaa agagctaaga
     8761 tattccaata gaaaatactc cttattatgg aagtaatatg aaatattaca attgaaaaat
     8821 aaaaacaata taatggcgtc actaaaattc gtattttttt attataatat aacttaatta
     8881 caaatgctca tctatgaaca attgagtcct aattagggag cgtcaataat tttgtgtaaa
     8941 taaattgtcc tcct
//
LOCUS       DACDEG010000099         8906 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.103, whole genome shotgun sequence.
ACCESSION   DACDEG010000099 DACDEG010000000
VERSION     DACDEG010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.103"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(263..2644)
                     /locus_tag="IV142_002354"
     CDS             complement(263..2644)
                     /locus_tag="IV142_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP8485957.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2634..3281)
                     /gene="recU"
                     /locus_tag="IV142_002355"
     CDS             complement(2634..3281)
                     /gene="recU"
                     /locus_tag="IV142_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP8485958.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3345..3884
                     /locus_tag="IV142_002356"
     CDS             3345..3884
                     /locus_tag="IV142_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP8485959.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3974..4393
                     /gene="gpsB"
                     /locus_tag="IV142_002357"
     CDS             3974..4393
                     /gene="gpsB"
                     /locus_tag="IV142_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP8485960.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4415..4782
                     /gene="rnpB"
                     /locus_tag="IV142_002358"
     ncRNA           4415..4782
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IV142_002358"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4991..6157
                     /locus_tag="IV142_002359"
     CDS             4991..6157
                     /locus_tag="IV142_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP8485961.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6203..7699
                     /locus_tag="IV142_002360"
     CDS             6203..7699
                     /locus_tag="IV142_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP8485962.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7780..8463
                     /locus_tag="IV142_002361"
     CDS             7780..8463
                     /locus_tag="IV142_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8485963.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         2815 a         1597 c         1882 g         2612 t
ORIGIN      
        1 ttattaagaa gtggcactaa ataaaagact agtgtgtatt tagccgattt aattataaac
       61 aagtaaatgg ataaagtata tgtaatgata aaagaaaaat ggattttcta atttttaagc
      121 taaatgttag atcacacatg tatatgctgg atagtcttat aaaaaatcat gtaatacagc
      181 ttggtacatg atttttattt gtagctgttt tttacataga cacaaaaaag aaaccacgct
      241 tgtagacgtg gtttcttttt tactatctgt ttccagaagc tgaggtgctg ctggatggtg
      301 tatggtcatt tgcagcatcc gctccgctag ttgctggctc actggaagaa gcaggaggag
      361 cagatgaaga cggtgtcgag ctttcttcgg atgaagaaga aggaaccgtt gttgaagacg
      421 ctggctcgct tgtttctgct gtcgtgcttt cttttgagga ctcagcagtt gattcgcttt
      481 gactagtaga gctagagctt tgagtcgttg tctctgttgt tgagcttcca ggagtctgga
      541 catagctgga agatggaata gttgttgatg gtgtaattgc attcgatcta gctgtatatt
      601 gatctttgta gtacagttca ccgcccacac gaattaatcc gcttggcatc tcccagtctg
      661 tatttgtcac gttagctgaa acatactgca taagctcgcg ataaacatca gaggctacgt
      721 gtgacgattc tgaagtgaca ggtgtcattt ttttattgta acctgtccac acggaaatcg
      781 aataatttgg cgtatagccc gcaaatagga tatctggata caccccagaa gaaataccaa
      841 gttttgcata ctcgtcatct gtgtaattgg atgttccggt cttaccggct tgatataacc
      901 cagcgatttg cgcattcgta ccagttcctt cagtgatcgt atcttttaaa atgtctgtga
      961 tcatataagc tgtttctgga gacatggctg tttttccatc tggctcataa gtttcttctg
     1021 ttccgtcttg gaagacgatt ttattaacat attgaggttt gtaataagtc cctccattgg
     1081 cgaaagccgc ataggctgct gccattttta aggaagaagc tccgtatttt gtcccatctt
     1141 gttcttccgt attactagag atcgcattcg attgatggat cgtactgtat tcgatcccaa
     1201 gattttttaa gaaagaagcc actttatctg aaccgacttc attgaataat ttgactgctg
     1261 gaacattacg ggataagtaa agtgcttggc gtaaagtaat ggtgcccata tattgattat
     1321 cccagtttcc tacaggagtc gaagtccctt catagttata cggtgcgtca gtaatagttt
     1381 ttccagtaga atatttcaaa tattcaaaag ctggtccgta atctgtgaca ggtttcatcg
     1441 tagaaccaaa atcacgggaa gtattcactg ctaagttgtt gcctaaagtt acgtcttctg
     1501 cgatgtgacg tccgccaatc tgggctttta ctttcccggt attggtgtcg ataagggtgg
     1561 aggcaacttg catctcgtcg tctgggtaag agacatactg atccgtgttg acgatatcat
     1621 atagtttctt ttgggcgtcc agatccaaat tcgtataaat ctctaaaccg tctgtgtaga
     1681 cattcttatc tgttttttct tgtacttcat tgatgacttc tttgacataa ttatcgacga
     1741 tcttagtatt gtcatcggat tgagttagtt cttgcagacc atccgttacc ggtacattaa
     1801 cagcttgatc atattctgtt tgagagattt tttcgttttg aagcatcgta tacaaaacag
     1861 tatctcgtct tttcttcgcc tgatctggat aaacataagg atcataggcg gaaggggctt
     1921 gaggcatccc agcaagcaat gctgtttgcg gtaaagaaag ttctgataat ttcttaccaa
     1981 agtacatctc agaagctgtc tccatcccgt acaagccgtt tgacatatac actttattta
     2041 cgtagtaggt caagatctct tgtttcgatt ttttttgttc caatcttaca gccatccacg
     2101 cttcttgggc cttgcgtttc agagtctgat cctcggcact ggtggagaag aaggaaagtt
     2161 tgatcaattg ctgtgtcaaa gtacttccgc cttgcagtcc gccagaggta aagttagaga
     2221 gggcagaacc gatgatccga atagggtcaa ccccgatgtg tttataaaaa cggcgatctt
     2281 ctaccgacac gatcgcatct tctaatgttt tcggtaattc attcgcagaa attttttcac
     2341 gcttttctgc gcctaaatct tcgaatagtt cgccatcttg tgtatatagt ttggaagata
     2401 cggtagcgtc taatttttta tccgtcaatt cgggcgcatc ttttgcgtaa taccagaaaa
     2461 gtcctacacc tgctaaaaag aggatacaga aaaatgagag tattcctaaa aggatcttga
     2521 taaataatcc ttttttatgt gttccagagg actttccaga acctttgcca gaattcttct
     2581 tcacactctt tttaggagta ggctgtttct gtcgtctaga gcttctagtt tgttcatttg
     2641 ccattcttgc tttctccttc aatagataat gttgataaat agaagtcaac cgcgtccaaa
     2701 taaggaatcc gcggagaaat accagttttg atctctattc ctagttcttc gatcaaagat
     2761 aaaggtagtg atttgcgtcc tttttccttt tgggcgtccc agtaagcaat caaatgaaca
     2821 ctattcaaat aaaaacagcg cttcaaagac gaaaaccata acaagacaaa gcagacccca
     2881 ttttgttcga ggcatgcttt catatgttcg atttgatggg catggaagtt tttaaaggga
     2941 aaagatgttt tgttcttggt ttcttttgct tcaaagtcca agtaaaagcc tcgatagacg
     3001 ccattatagt ctgtagttga ggcttgggta aagtaggctt ccttgatcac agcagcactt
     3061 ctcttaggat agtctacttt cacgatttgg ataggggtag gctttttatg gataaccgcc
     3121 aacccgcggg aaagataata tttattactt tcattgattg cttcttcaaa gcgcatcccc
     3181 cgattgccga attcagtgat ctgatttctt ttttcttgaa ttttttcgac gggagaggca
     3241 ttcttataat aaggttggcc atttggatag cgcaatacca tctttaatca cactcctagg
     3301 ccacattata gcaaaaaagc ggatagaaag aaaaggaatc tgagatggat aagtcaaaag
     3361 taatctatgt gtctgggtat cgaagtttcg aactcggtgt ttttaaagaa aatgatccta
     3421 aaatccaagt tgttaaaaat gttttaaaaa aagaactcca tcagttagca gaagaagggc
     3481 tggaatgggt acttgtcagt gggaatttgg gcgtcgaact atgggctgtg caaatagtag
     3541 cggaattgaa agaagaatat ccggaactat ctcttggtat tttgtatcct ttttctgatt
     3601 ttggtaataa ctggaatgaa gggaaccaag aaaaaaaaca attagcagaa caattagcag
     3661 attatgtcga agctgttagc catcaaccct accaatcacc tagtcagcta aagaatcaca
     3721 ctcgttttat acttgagcat acagtgggct gtctgctgat ctatgatgaa gaattccctg
     3781 gaaaaacgaa gtattttttt gaagatgcac agaattacca agcagatcat ccctacgaaa
     3841 ttcggctgat cagtatggac gatcttcaaa attttagtga ataatcgata ttgtgtttga
     3901 tttatcaact tttttcctat acaatgaaaa aggtagaaat gttattacgt aaatgaaaac
     3961 tagaggtgta aatatggcga atttggttta tagtcctaaa gacattttac aacaagaatt
     4021 caaaacaaaa atgcgcggct atgatcccgt tgaagttgat gaattcttag acaatatcat
     4081 caaagattat gaaacttaca gcaaagaact ccttgcttta caagaagaaa acgatcgatt
     4141 gagtgcaaaa gtagctcaat tatctaaaac tcaaggagca gctcaaacac gcgtgcaaca
     4201 aacagaggca ccaaagagcg cagcagtgac caattttgat atcttaaaac gcttgtcaaa
     4261 tcttgaacgt gaagtatttg gtaaaaagct tgatcaacaa gcaagtgcag tcaaaccagc
     4321 acaacctaat ccaaataact acacaaatgc agacacaagc ttagatgata atgaaaaaac
     4381 acgtcaattt taatttttga ttaatgaatt tgcggttttc gggtaatcgc ggtctgactt
     4441 gatcaggctg aggaaagtcc atgctcgcac aggctgtgat gcctgtagtg ttcgtgctta
     4501 gcgaaaccat aagctaaggt actctttgga gtaacggcag gaaaacaagc taaggcttcg
     4561 gctatgctta agtatccttg aaagtgccac agtgacgaag cggctgggga aacccatccg
     4621 gtggaacgcg gtaaacccct cgagcgagca acccaaacaa tggtaggggc gctttttgtc
     4681 tggaagaaga acaggacaaa aaggcagtat aactgcagat agatggttac cgctttgttt
     4741 tttctccctg gaaaagcaaa gtacagaaca tggcttatag aaaaccgcaa acatcaggga
     4801 attaggtcgt gacaaaagta atgcgttata agaaacaaga aaaacatttg aaagctattt
     4861 ttcaatactt cagatgaatc gttcttatta caaaacaaac ttttgtatca ccggttattc
     4921 ggaatttgag taaaagtgtg gggtaggact gatgtcccac ctcctttttt tataaataag
     4981 gagattgtgt atggaaaaaa acaggaagtt caacctcgtc gcaactgcgg caagcgggct
     5041 agaagcactg gttggtaaag aattaagaga tcttggaatc gaatgccaag ttgagaacgg
     5101 acgtgcacgt tttgaaggaa caatggaaac gatcgctaca gctaatttat ggttacgtac
     5161 agcagatcga gtaaaaatcg tggttgggga attcgatgcg tatacgtttg atgaattgtt
     5221 tgaaaacgta aaagcattgc cttgggaaga ttttcttcca ctagatgcag cttttcctgt
     5281 agctggaaaa tcgatcaagt cgaaactata tagcacacct gattgccaag cgatcacaaa
     5341 aaaagcaatc gttgaacgtc tccgtaccta ttatcaccgt ccagcttctg taccactgac
     5401 ggaaactggc gcacattttc agttggaagt agctttgctg aaagaccatg tgatggtaac
     5461 tctggatacg acaggaccaa gtttattcaa acgtggttat cgcttagaaa aaggtggagc
     5521 accattgaaa gaaaacatgg cagcagcact tattatgttg acaaattggc gaaaagaccg
     5581 tccattttat gatcctgtat gtggttcagg tacgctttgt attgaagcag ccttaatagg
     5641 ccataatatc gcaccaggat tcaatcgtga gtttgcttgc gaatcttggg attggttcag
     5701 tcaagaaatc atggaaaatg ttcgatcagc agccgaagaa aaagcagatt atgatattga
     5761 attagatatt actggttcag atatcaatgg acggatgatt gaaattgcca aagccaatgc
     5821 agaagaaatc ggattaggct cttcgattac ctttaagcaa caagcagtca aagacttcaa
     5881 aacggataaa gaatacgggg tcatcgtggc taatccgcct tacggagaac gtctaggaga
     5941 agaagaaact gttcggaaat tatataaaga aatgggcgat gtattccgtc cattaaaaac
     6001 atggagtaaa tatatcttga ctagcgactt agcgtttgag gaattttacg gacaaaaagc
     6061 aaccaaaaaa agaaaattgt acaatggtgc attgcgtacg gatctattcc agtattgggg
     6121 aactcgtccg cctagaaaaa aagagaaaga cgaataatct tactaattga ttcattcaat
     6181 tggatgtttg gaggaatagg gaatgaaaga aaaagagttt ttacaagaag taaaagaaat
     6241 caatcttcta caacaagctt taggaatcct cgattgggat acacaaacag gtatgcctga
     6301 aaaagctagt gcataccgaa gtgaagtaga cagctatctt tacagcattt atttttcaaa
     6361 aaaaatcggt ccaaaaatca aagaagccat tcgctatttt tctgaacatc cggaagaatt
     6421 gtcggaagtt ggaactgccg tttttgccaa agtaaaagaa gagtatgatt tggaacatcg
     6481 cgtaccggaa gatcttatga gaaaatatac agctgccgtt tcttcagcac acggacaatg
     6541 gcaaaaagct agagaaacac aacaattttc tgattttcaa cacgcattga atcaaaatat
     6601 tgaattaaca aaacagttga taccttactg gagaaaagat gaaaagacga attatgatgt
     6661 tttgctgaat caatacgaac ccgggatgac tgtcgaaatc cttgataagg tatttgatca
     6721 gcttaaagaa ggaattcttt cgatccgccg tacgctccaa gaaaaaggga ctgaacctga
     6781 aacagacttt ttaaatcgga gaatgacgaa aaaacaacag aagagatttg tagtgaaagt
     6841 aatcgaacaa cttggttatg attttagtcg tggtcgttta gatgacactg tccacccatt
     6901 tatgactgga attaatccga atgatgcacg aatcacgaca cgatggaacg aaacagattt
     6961 caaaatggct gtttttggag tgatccacga ggctggtcat ggtatgtatg aacaagacat
     7021 cgatgataaa tatgcgtata caccggttta tgaaggtaca tcaatgggta tccatgaatc
     7081 acaatcgttg tttaacgaga tcattattgg aagtagtcgc agcttctgga aaaagcaata
     7141 tcctttcttt caagaatgtg cagaaggaac attcgatgat attccttttg acaaatttta
     7201 tcgttcattg aaccatacaa aatcaagttt ggttcgtatc gaggcagaca gtttgactta
     7261 ccctctccat atcatcattc gttatgaaat cgaaaaaatg attttcaatg aaggggtaga
     7321 tgttgctgaa ctacctgaaa tatggaatca aaaatatgaa gagtatctgg gaatacgacc
     7381 aacaaatgat gtggaaggca tcctacaaga cgttcattgg tcaggtgcaa gctttggtta
     7441 ttttccctct tatgcattag ggtacatgta tgcggcacaa ttacttcatt ctttgcagca
     7501 agaaatagat gtggaaaatg ttctagcgtc agacgattat tctccaatca aaaactggat
     7561 gtcagagcat atccatcaat atggtgcatc tagaaaacca aatcaattaa tcatggatgc
     7621 aacacatgaa ccgcttaatc cgcaatacct gatcgactat atgaaagacc tgtattttaa
     7681 tgtatatcag gtaaaataaa atacaattta caaagatagc tgaaattcat ctaatctata
     7741 gatgttttca gctatttact taagaaagaa gtggaacgaa tggataaatt acaagtgatc
     7801 gctgattata gtatggacca attagcgcat gatcaaacag gacatgggag cgatcatacc
     7861 aaaagagttg tcaagttagc tgaaaaaatt ttggagacgg aaccgcaggc agaccgcttt
     7921 gtgacgcttg cagcagctta cctacatgat acgattgatg ataaagtcgt taaaaatgag
     7981 aacgaggcaa agcaacagct tcgtgtattc cttcgctctt tgccaatagt agaggaacag
     8041 atctcgatga ttttttccat tatcgaaaat atgtcctttt ctaaaaatct ctctgaagcg
     8101 gccgaactat ctctggaagg taagatcgtt caagacgctg atcgtatcga agcattagga
     8161 gcaatcggca ttttgcgtac tgcttatttc ggcggtggac atgggcaccc gatttttgat
     8221 cctgaactgt atccgcaaac attcaaggac aaagaaaatt accgaaaagg cacgacagtg
     8281 atcaatcatt tttatgaaaa actgttcttg ctgtctgata aaatgaatac tgattacgga
     8341 tatgaagaag ccaaacgaag agaaaccttc atgcgtgaat tcttagaaga attttttctg
     8401 gaatggtcta ttgacgatga aaattttact ccagacaatt ggatcaaaaa taacaaaaaa
     8461 tgatggaggt tgtgacagaa gtggtcagct ccaagatatt tcggatcatt ttccgtaaaa
     8521 gttacttctg gaacactgtt taccaagagg tcgtgagcct gccgttcagc tccgagtcac
     8581 aaggaacaat ttcaaaaaat agcttctcgt gtttgatgaa tattgtaact tgtgattcgg
     8641 aattggcagg agaacaccgt ttattcggaa gaataggact gtgacaagac ttttgtcaca
     8701 gtcctttttt cgaattcaac aagtaaaact tttaagaaat attcagagag taaaattaaa
     8761 aattgctgat aaacgaagat ggaaatttta tgtattttta ttggtctatg caataaataa
     8821 attaaatatt cagaatacaa ttgattttaa cgaaagggct tctatcaatg gttgtgttgt
     8881 atttatctgc ccaggtagag taaaat
//
LOCUS       DACDEG010000100         8885 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.104, whole genome shotgun sequence.
ACCESSION   DACDEG010000100 DACDEG010000000
VERSION     DACDEG010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.104"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..448
                     /locus_tag="IV142_002362"
     CDS             <1..448
                     /locus_tag="IV142_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485964.1"
                     /translation="FLSKTIPRYSKNKKAEKFCLLLNKFTLIAPILAFIIFSVLLSTT
                     LKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKPPKSISLCLVINGIFLLSLIIFITP
                     LDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNNNFYFYRNKL"
     gene            complement(574..1251)
                     /gene="nth"
                     /locus_tag="IV142_002363"
     CDS             complement(574..1251)
                     /gene="nth"
                     /locus_tag="IV142_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP8485965.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1258..1956)
                     /locus_tag="IV142_002364"
     CDS             complement(1258..1956)
                     /locus_tag="IV142_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP8485966.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2224..3408
                     /locus_tag="IV142_002365"
     CDS             2224..3408
                     /locus_tag="IV142_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP8485967.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3414..3857)
                     /locus_tag="IV142_002366"
     CDS             complement(3414..3857)
                     /locus_tag="IV142_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8485968.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(4083..4370)
                     /locus_tag="IV142_002367"
     CDS             complement(4083..4370)
                     /locus_tag="IV142_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485969.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4541..5893)
                     /locus_tag="IV142_002368"
     CDS             complement(4541..5893)
                     /locus_tag="IV142_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP8485970.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(6166..6612)
                     /locus_tag="IV142_002369"
     CDS             complement(6166..6612)
                     /locus_tag="IV142_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8485971.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6848..7774)
                     /gene="cysK"
                     /locus_tag="IV142_002370"
     CDS             complement(6848..7774)
                     /gene="cysK"
                     /locus_tag="IV142_002370"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP8485972.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(7914..8801)
                     /locus_tag="IV142_002371"
     CDS             complement(7914..8801)
                     /locus_tag="IV142_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8485973.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT         2430 a         1805 c         1457 g         3193 t
ORIGIN      
        1 ttttttatcg aaaacaatac ctcgttattc caagaataaa aaagctgaaa aattttgttt
       61 attattaaat aaatttactt taattgctcc tatccttgca tttattattt tttctgtact
      121 tttaagcact actcttaaag gaaaatttat ggaaagaagc tctcatgcaa tgattctcac
      181 ttttttatgg ttgttgttca ccagaatcta tatttttctg atgagcctga aaccgcccaa
      241 atccattagt ttatgtttag ttataaatgg tattttttta ttatcactta tcatttttat
      301 tacaccttta gatagatacg tcacttattt atataatcct ttggaatact ggacttattt
      361 tataggtata ttggaaggca ttattttcta catcgggtat tttcccaata aaaataacaa
      421 cttttatttt tatcgcaata aattgtaata agttaccatc ctagctactg agtatgtaca
      481 tttacttttt catgcaaaaa agataaacga aacagctttt ctccataata acaagataat
      541 tcatacattt atttgcaatt aaagaaacca cgtctacagg cgtggtttct tttttagcgc
      601 cttttctttt agacgcatcc gatttttccc atcttgacat attgatagca aaggacatac
      661 ctcgcacttg ggatttcttg ccgtgcaatg atatctacca aaaaaaatca gtgtatgatg
      721 tgtctttacc cataattctt gtggtacttt ccgcatcagt gtttcttcta cttccatgac
      781 cgtagcatct agtttgcaga tcctcaggcg ttttgagaca cgttccacgt gggtatctac
      841 tgcgattgct ggtatcccga atgcatcccc caatacgaca tttgctgtct ttctgcctac
      901 accaggaaga gacatcaatt cttctcttga cgttggcact tgcccatcga aacgttcaat
      961 cagctgctgt gcacacgctt tgatgttttt tgctttattt cgatacaaac caatcgtttt
     1021 tattttaaga atgatttcat cgattgaagc ttctgctaac gcatcgggtg tcggaaaaga
     1081 ggcaaacaaa tctggcgttg ctttattcac tgatacatct gtcgcctggg cacttaagat
     1141 cactgcaatc aataactcaa atggattatt gtgcttgagt tctccatgtg cttcgggaaa
     1201 cattccatac atcgtttcta gagcttccat tgttttttgt ttgcttaaca tattccgcta
     1261 ctcctccaac caatttttca gtgagacttt cggcaattct tcttgttgag ctgactgttc
     1321 tacttctctt tgaagaagct gctgttttct tcgtttttgg tcttcttcca cttgttgttt
     1381 tgtacgtaga ttttttcttt cccacgatag caaaatgcgg tcaacgtaat taaaactata
     1441 agcctgattt aatacggctt cccgcagtgc tagcttcaaa atttccggct gataatggtc
     1501 ttcttctagc cattgtccga tccgctggaa ctcgatcgaa gaaagcgggc gtccaaattc
     1561 ttgttcaaat aattgataca ctgactggat ttctttttct tgtgtttttt cttcgtattt
     1621 tttttcttgt gtttttaaat actcacctaa tagatcatag atggggtaaa gattatatcg
     1681 atctgctttt ttaccgttga caatcgtcgt ttctatcttg ataaatccat ttgttacgag
     1741 acgatccagt atttgaaaaa tctgatcttg tttgattccc atatccatag caatcatctg
     1801 caaatcaggg aaagtatctc cttcaagctg tgccatatgc aactgtagaa caaataaaaa
     1861 ttcttcagat tgtagaccga tcctatgata atttttaagt aaaagattag atatgactgt
     1921 ttgtccagcg cttaagtaat tttctaaatt caacatgatt tctcctccgc tcaatcacag
     1981 tatacgtaaa aaaaacatgg gaagcaagaa caggtttctt ttctatttac ttcactgatc
     2041 ggtcagattt acaatgtcgt ctacttgatt tttgtttgtt catgtttttg atgcgtatct
     2101 tcacttttcg gaataaataa attaagtaga tttcgctgtt ccttagaatg ttgacaagga
     2161 aacaaaatta ctatacacta tttttgtttc caagttaaca ttattgcata aaggagtttt
     2221 tacatgtaca gtttcacaaa taaacattct aaagtttttg gatacgtctc tgtcttattc
     2281 actggattag gatttactat cgtcagtcct gtacttccgt ttttaacgct gccttattct
     2341 cactccatac atcagcaagc tttctatatc actttgctca tgtctgttta tgctctagct
     2401 gccttcttat ctgcccccat tctcggtagc ttaagtgatc attttgggcg tcatccgatc
     2461 cttattatca gtttattagg atcaagtatc ggatatttgc tttttggttt aggaaattcg
     2521 atatggatgt tgttccttgg tcgtatcatt gaaggattga cggcaggaga aatctccacc
     2581 ttatatgctt actttgccga catcacggaa cctaatgaaa gaaccaaagt ttttggatgg
     2641 atgggtgctt tagtcggtat aggaacaacg ctgggtccta ttataggggg tctattagct
     2701 gagttgggga attctgttcc tatatttatt ggagcactct ttactttctt aaatgccgtt
     2761 tatggctaca catttatgct agaaagcctt ccaataaaaa aacgttcagt agatttaagt
     2821 ttctcccatg taagaccttt tcaccaatta caacaattat tcaagatttc atccgttatt
     2881 cctttattga cagcaggttt tgctgtttgg ttggcagctg gctctcttca atctatcttt
     2941 tctcaatttt cgattgacgc ctttcagtgg aaggctgggt tggttggttt aagcttctct
     3001 ttgattggaa tcttggatac tgtctcgcaa ttgttcatta tgcctcgatt gctcaaaaaa
     3061 ttttcagaac agcagatcac aagaataggg atgttcagtg aaatcctcgc ctatctattc
     3121 atcacattat ctggaattct tctactgccg attttgtttt tatttgggat tatctgttat
     3181 ggtttcggtg attctatttt tactcctgta ttcaatggcc aactatccaa ttctgtctca
     3241 gataaccagc aaggtcttgt aatgggcggt acccaaagta tacaatctct atcgcgcgtc
     3301 atcggcccgc taatcgcagg acagctttat gctgcagcac catttcttcc gacggtattc
     3361 ggatttttgc ttatgatttt tgcctattat cactacaata aaaaatagta gcttcatttg
     3421 ctacttcgta taaattctct gtatcgaccg ataaaacgaa atactgtgtc aaattcttct
     3481 tttgacatat tttcaaatac agtagcatct cgctgtaccc atttttgatg gagttgctcg
     3541 tgtctattcc ttattttttg tccgattggc gtcaattgaa aatagatttc ttttttattc
     3601 tcttcgcttt gataagtgtt tatgatccct ctagctatca gtttttttgt tagcttactg
     3661 atggctcctc tagtcatgta actagcattc gctaattttg tcacgttagg atcagggatt
     3721 ttcccgatat gttcaatcac ttctagttca tttagagtaa atcctgcaaa ttctttttcc
     3781 atttctggtt tattacgatg aataatctca ttagctaaat ccagatattc ttctattagc
     3841 agttgtcttt tatccatttc tacaccttct ttttctttca ttatattgtt tttttgtttc
     3901 ctattcaaca aagataaaaa tattttgtag gaaaacccaa tctgaaacca agaaaaaaag
     3961 gggttgacaa agcttgtcat aacccctttt tctgattaaa agatagtaaa gagacagtca
     4021 tcggtaattg tgatgatttt ttgtcatcac ttaaggatga gcggcctttt caaaaactgt
     4081 ctttaaagat catcatcagt ttctagcaca tatggctgac cttcatatgt gtactctgta
     4141 ttcaatgtgc cgtcattttc atctgagtag atgcgtctac tgaaaggaaa acgatgagaa
     4201 acattatctt tggcgtcatt tgctggcatc gtgtaatatt cctcttgcgt ttcattcaac
     4261 tgagagagta cttgatctgc caactcatca atattgatcg ttaatttctt cgaagcactt
     4321 ttataatctt ctaaaaatgc atcttgttgg acaaaatatt ctttattcat cttctttctc
     4381 acctttcttt tcaaaatcaa ataaagctgc ttgttctttt atgtaacagc tttatctttg
     4441 ctgcttattg tgacaaaaaa ttcgaagaaa gacaactaat tgtacttttc acaaaaaaag
     4501 acagagggtc gtgacaaaat tgtgtcacaa ccctttttct ttattgtgca gtttcgattt
     4561 cgcctagcgc tgcttgtgca agtaggttca agtaattcca tgggcggtca aaatgtggtt
     4621 ggaagaagaa atctgaaagt gcgagatctt ctacagtcat tttattttga acagctaaag
     4681 acatggtatt tgctgattgt gtaatgtcgt atttggacat gaactgagca cctacgatac
     4741 gattcgttcc tttttcatag actagctcca tcattacttt ttcagtagtt ggcatgaact
     4801 ctggacgata attatcttca aatacagtcg cttggacttc tagatcgttc aaagttgcgc
     4861 tttctttggt cacacctgtc gaaccgatcg tccagccaaa taggtaaagt cctgaagttc
     4921 cttgagtacc acgatacgcc attttatgat ctgtcaagtt atttcccaca agcaaacctt
     4981 gacgaacggc atttgttgcc aaaggaatat agttttgcgt gccacttgga ttgtagttga
     5041 ctactgcaga gtctccagct gcaaaaatgt ctggattact tgtctgcatg tattcattca
     5101 ctttgattgc cccgtttggc agtgtatcta cttttccttc gatcagtttt gtatttggac
     5161 ggaagcccac acataaaatg accatatctg tatcaaatgt atctgttggt gtagctactt
     5221 gtttcacttt tccttcttca tctgtgataa attcagtaac attttcgcct agcgcaagag
     5281 taacaccgcg gtctgtcaat tctttttcta agatatctgt gaatggttta tctagatatt
     5341 tattcaagat gcgatctaat ccatcaatca atgtcacttg ttttccagat tcgacaaaag
     5401 cttctactaa ttcaataccg atatatccac caccaacaat tactacacgt tttgcttctt
     5461 tcgcttgtgc aataatttca tttgcttgat tgtagttttt gcataggagt acatttttcg
     5521 catcgattcc actaatcggt gggataatag gccatgaacc agttgtcatc actaatttat
     5581 cataagaaac cgtttctgtt tctcctgttt tcagatcttt tgccgttact gttttatttt
     5641 ctgtatcgac gtctgttaca tcgtgttcca ttttaactgt tgctcctaat gaagataatt
     5701 cttcaggatt tgagtagaac aagcctgctg ggtcctttac tactccgccg acataaagtg
     5761 cgataccgca agataagaaa gaaatattgt catttctttc atatactgtc acttctgctt
     5821 ctggatgatt ttttaaaata ctttttacag ctgcagtacc tgcatgagta cagccaatta
     5881 ctactacttt catttatggt ccctccgatt aacattttgt ccttcgacaa aactactata
     5941 acagaaataa aacggataca acagtttaat gcttgtgaat tcacaaaggt ttgtttgtag
     6001 tttcattcct tttcaagcat aagaacccaa tatctacaag attcttattt taataatatt
     6061 cacttttata tctaagattg tgaataaaag acaaaaaaat aaaaaagcta gcgttttttt
     6121 tacacagcta gcttttttta ggtataacac ttgtagaaac tgtttttaca tttgcgcctt
     6181 ttttgctgac tctttacaat ccggacagag tccatacaat tccaaccgat gttcattaat
     6241 ctcaaaatca gtcagtctgc ttgcagccat ttctatatct tcaagccctg ggtaatgaaa
     6301 atcaacaatc ttaccgcagt tttcacaaat tacatgataa tgacgctttg aactgaaatc
     6361 aaatcggcta gaagcatcgc cgtacttcat ttcttgaaca aatccaatcg aagtaaataa
     6421 gcgaaggtta ttgtaaacag tcgcaacact catatttgga aaccgatgtt caagtgcttt
     6481 ataaatctca tcagccgttg gatgggaatg atgttcaatc aaatattcta aaaccgcgta
     6541 ccgttgaggt gtgattcgaa tgttctcttt ttttaacttt tcgataacct cttcgactaa
     6601 cttattttcc atgtaaacac cccacgttgt tattctcgtc ttatcatact atttttacat
     6661 tttaaaatcc acttaacatg aaaagaaaat tcaattttat tgtattttaa tgaaaaaaga
     6721 cgtaaaacag tcgcgttcac tattctacgt ctctattcaa ataatttttc tcatcataaa
     6781 tttttgtgtg ttcttttcat ctttaaaagt tttttacata aacctatctg gagaaaaacc
     6841 aagtgcctta tatctcttgg taaagggctg tagataaata acgttcacca ttgtcgggaa
     6901 ccacagctaa taccttcttg ccttttccta attcttttgc gacttttagc gcggcagcaa
     6961 ttgcagcgcc agatgagatg cctactaaaa tcccttcttt tcgcccaact tctctagctg
     7021 tttccatcgc ttcgtctcca ctaatagaaa ggactttatc atacacagaa gtatccagtg
     7081 ttttaggaac aaaacctgta ccgattcctt ggattttgtg aggtcccgct tctttccctt
     7141 ctaaaatagc agattctgct ggttctaccc cgattaattc gattttcgga tacacacgtt
     7201 ttaattctcg gccagcgcca gtgatcgttc ctcctgtacc gatacctgca acaaacgcgt
     7261 ccaagccatt tactccaaat gcttgatgta tctcaggacc agtcgtcttc tcatgcacaa
     7321 gaggatttgc ttcattttca aattgtaatg gtaaaaagta gccgttttct tttgccaaac
     7381 gttctgcttc tctgattgat ccagaaatac catccgcacc aggagtcaaa atcaactctg
     7441 cgccgtaccc tttcatcaat aaacgtcttt cgatactcat cgtctcaggc atcacgatga
     7501 ttactttata tccttttgca actcctacca tcgacaatcc gattcctgta tttccagaag
     7561 taggttcaat gatagtatca cctggtttca gtagaccatc gtgttctgct ttttctatca
     7621 tacttaaagc aatacgatct tttacacttc ctcctggatt aaaaaactca agctttacaa
     7681 aaacatctgc tgaatcttct ggtacgattt tatttaattt gacgattggc gttttgccaa
     7741 tcaattcggt gactgagtta tagatagctg tcatcttcaa ttcctccttg tctttgctct
     7801 tcttacttat cataatacaa tctgttcatt tatggcgagt gattcgtctt tgactagcaa
     7861 aaaaaagaca aagatatacc atatatggta atttctttgc ctttttatct aaatcaaacg
     7921 tttagttctt gaccaactaa taaagaataa ctggacatag acatgccatt tttcgccatt
     7981 agatcatcaa cagacatgcc aaatttttgt gcaatatccc atagcgtatc acctgacacg
     8041 actgtatatg tctgaccatt atttgtattt acatctgttg ttgaagatga tgacgttgaa
     8101 gtcgtcgtag tagtcgttgt tgttgtactt gtggttgttg tagccgaacc tgttgatggc
     8161 gtatcaaaac gggttaaatt ataagtatta atcaatgagt tcaatttggc cccataattt
     8221 ggatcggtag cataacgacc tgtcaagtac gctgttgcat cataaaaact tgttgtattg
     8281 cttttccaca cgccagcata atgataatcg cccgtggaaa aactggttga acgaagaaca
     8341 ttcgcatggt cttggaagga ttcccaataa gaagaatatt ttcggaatgg ctcattcatc
     8401 gttacccatt gtccgtttac atattctttg gttgccatat aaacgacctg tccgttatag
     8461 cttcctttca caccgaatag attataattt ggcgcactag ctaatccgga agtgccatat
     8521 gcactttcca acaacgcttg agcgatcatg actgaagcat acaagtcatt acttgcagca
     8581 acttctgacg cagaccagcc gatttgatcg ataaacgctt gaggatcagc gactgtttga
     8641 gatccttgtt catctgcatg aacatgttgc ggtaccgctg taaccatcat cggcccaact
     8701 aaaagactcg ttccaattaa aggagcaatc gcttttttat tgatcaccat ttttctttta
     8761 ggtctatggt gccgggcact gcgggtcttc agctcttgca ttcaaattcc tcctcaaaat
     8821 aaaaaaactt aataaaaagt tctcttctta aaacaaactt aattaattaa attatacttt
     8881 taatt
//
LOCUS       DACDEG010000101         8350 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.105, whole genome shotgun sequence.
ACCESSION   DACDEG010000101 DACDEG010000000
VERSION     DACDEG010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.105"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..365
                     /locus_tag="IV142_002372"
     CDS             <1..365
                     /locus_tag="IV142_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485974.1"
                     /translation="EKVRYKLPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKP
                     YSLFAMYYTFSSFLFLEMLSDKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKR
                     EFPNSGKAWLINIGQFFI"
     gene            378..2396
                     /locus_tag="IV142_002373"
     CDS             378..2396
                     /locus_tag="IV142_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP8485975.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(2456..3172)
                     /locus_tag="IV142_002374"
     CDS             complement(2456..3172)
                     /locus_tag="IV142_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP8485976.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(3399..3941)
                     /locus_tag="IV142_002375"
     CDS             complement(3399..3941)
                     /locus_tag="IV142_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8485977.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(3946..4173)
                     /locus_tag="IV142_002376"
     CDS             complement(3946..4173)
                     /locus_tag="IV142_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP8485978.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(4252..6132)
                     /locus_tag="IV142_002377"
     CDS             complement(4252..6132)
                     /locus_tag="IV142_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP8485979.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(6321..6935)
                     /locus_tag="IV142_002378"
     CDS             complement(6321..6935)
                     /locus_tag="IV142_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8485980.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            7077..7637
                     /locus_tag="IV142_002379"
     CDS             7077..7637
                     /locus_tag="IV142_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8485981.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(7942..8256)
                     /locus_tag="IV142_002380"
                     /pseudo
     CDS             complement(7942..8256)
                     /locus_tag="IV142_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
BASE COUNT         2530 a         1645 c         1358 g         2817 t
ORIGIN      
        1 aagaaaaggt ccgctataag cttcctcaag tatttatcag cttgattgtt ttattcggtt
       61 tgattatttt gatgacacaa atttcactca tcatcagagg aaatacagat acagcgctga
      121 ggttttctaa accatattct ctcttcgcaa tgtattatac tttctcttcg tttttatttt
      181 tagaaatgct atctgataaa ttaggtataa aaggaagagg aaattttctt gctgtatttt
      241 tgattgtcac tcaagttata tgtaatttcc ctttagtttc ttatcgcgta tctacattta
      301 tgaaacgtga atttccaaac tcagggaagg catggctgat aaatatcggg cagtttttta
      361 tttgatattt attagcagtg tccctcctag ttatcttttt attttatata caaaaaatcc
      421 gtttcattca tcgtttcatc gaatacttcg cttttgattc gattgaacaa ttaactcagc
      481 gttggaaaaa aagaattcat tggctttttg ttcataaaag ttatttttta gttgcagcat
      541 ttttttactg ctttaccttc gtgcagattt tcgttttaga accaaaagaa ggttggaaag
      601 aaactatcca ggttgcctta aaaatattta ctcagcgaca agcacccatg atcctgacaa
      661 tcatcattat tatgggattt ttcttcttac tattgttgat ttccaatcgt ttttggtacg
      721 ccttgactgc taccttgatc attaacttac tgctcactat ttcgacagta atcaaaatgg
      781 aaatgcggga agagcctgtc tttccttcag atttaaaaat gctgaccgga ttgtcagaac
      841 tattatcgat ggttagccca gtcttattaa tcgtgggcgg attgatcctt ctcctattat
      901 tgattactag tattattgta caacgtagac tacaaaaaca gtattccttg aagattcatt
      961 ggaaacgtcg atttatcagt atcgctgtat tattaggatg tttttcgggt gtctttttca
     1021 ttaatcacaa aaattcgcct tctttcttgc tgtttaattt atttaaagta aacaagactt
     1081 tctttaacca gcaaggcgcc gtaaaagata acggaccagt cattcaattc ctaaacaata
     1141 tcgacattaa aatcatggaa gaacctgctg gctattcgaa agaaaaaatt aatggaataa
     1201 tgaaaaagta caacacaaag gcagatgaaa tcaaccaaac aagaagtgac tggttaaaca
     1261 atcagacgat cgttctcaat ttaagtgaaa gcttcagtga tcctgcacgt gtccctaatc
     1321 tgacagtacc tacagaccct attccttaca tcaaaagcat tataacagaa ggaacaggag
     1381 gattgatgtt atccgtcggt tatggtggcg gaacagctaa catggaatgg gaaggactga
     1441 caggcttgag tatcagtaac ctgtctgctt cgttagttac accttatacc cagctagtgg
     1501 agcgtcagaa aataagtcca aatattaccg atctattcga tgaaaaaata gccatccatc
     1561 cttttacagc tgctctttat cgcagaaaac aagtgtttga taaatttggt tttgacaggt
     1621 tctattattt agacagtccg gataaattaa cttatacgga taaaatagaa caaagcccac
     1681 gcatatcgga tgattcagca tacaaagaaa ccttgaaagc tttacaaagc aatacggaaa
     1741 cgtcacaatt catccaatta tccactatgc aaaaccatat gccttatgat gattactata
     1801 gtgaattgaa ctacactgct gaaggaactg ctgtcattcc taatagaaga aaagaattac
     1861 aaacttatat gcaaggcctt cactataccg atacagcagt aaaaaaattc atccaagaaa
     1921 tcgacaaaat cgataaaccg attacctttg ttttttatgg tgaccattta cccgcaatct
     1981 atagtgggaa tgattcgaaa aaatatggat tagaacagca ccagacagat tatttcattt
     2041 ataacaacaa gtatagtcgt gaacaagcag ctacgttaaa taaagaagtt gttgctccta
     2101 ataactttgg tgctatggct cttgagcatg cgaatatcaa agtaactcct tactatgcgt
     2161 tgttgacaga agtatctaat catctacctg cattaacaat tgatcctaca gaaagcttgt
     2221 ctaatcgttt taatggaaaa caggtctttg ttacagagga tagtcaaatt atccaagaaa
     2281 aaaatttaac tgagaaacaa aaagaaattc tagaagatta tcgtctgata caatatgatc
     2341 ttgttgcagg taaccagtac agtgcaacat gggcgacaca aaaaataaaa gactaacaaa
     2401 taaactgagc aaggggttgt gataaaagac atgtcacaac cccttgttta tctcattaat
     2461 ttgcttgtcc ataaacgatt tgattaaagc catttttcaa ttcattattc gctgtctcgt
     2521 tgaagtaaag ataattatcg ggattcccct gattttgttg tgtcaccaat acttttggct
     2581 gattttgctg aaacccgaat agatagacgt gctttttcag atatggttct gtctctgcat
     2641 cggaatagac ggctgtgatt tgatagtctg cttgacctgt cccactttct gaccattctg
     2701 cagaaacagg cactccacca atagccatct tccactcccc gttcaaaata gcttgaggga
     2761 ctttaagacc atataagtcc acactcattt gattcgtata acttttgtat tgttgtccca
     2821 ttgtttggcc ccatttggtc ataaatgcct ctaattgtgc ggatttgtct gcattccaca
     2881 gtgtttgttc aacttgttcg ctgctttctg aagaggaaga tgaagcctct acagaagaac
     2941 ttgaagtaga tgtttgagtt gtttcagtcg ttttggaaga ctctgacgaa gtagaggtat
     3001 ctgctgtttc tgtacttgct gcagttgact tgctctctgt gctagttgtt gtagtagttg
     3061 caactgttgc tttcgctgtt tcttttgttg attctttttt cgattcattc ccgttggtac
     3121 aaccagctaa caaaaaagct agaccaattg ttactaaaat cctttttttc aattaaaaaa
     3181 ctcctttcga ttactcccac ttctttattc tatcaaataa gcaatgtgat tttattgatt
     3241 tttcgtattt tctcatcaat ctttcaaatc ttctaaaaac tgagaagcac ccgctgatgt
     3301 tgtctgtaat acatcagcgg gtgcttcttt tattttaatc ttcgtgattt tccaatttac
     3361 ctcacgcttt atatttgtat ttgaagaaac atagactttt agtcatcatc gtcttcctca
     3421 tccaaatctt gtcggacagt ttttccttgg agtctttgta cttggtagat gatttcttta
     3481 ttgaaactta aaagttcaac aagcaattgt tgcaagccat atttttcttc tttttcagct
     3541 aatttgattg cccgtgtgat aaagacattt tggaaatcaa aatcttttgc aaagttttcc
     3601 aacattgttg aggctggtaa atatttgttt tgaccatgtt cgtaaatttt tccataacgc
     3661 atgacttctt ctaaagtaga cggaacgatt tcattttcaa tcactaactc atgagagatt
     3721 ttattgaacc atctcgcttc ttgatcagca tattctgaaa aaagggtact taattcttgc
     3781 gaagatgtac ccaaaacata caattgtgct tggatcaatt tcatttcttc taatttaaaa
     3841 tttgctaata catgccccac catggcgcca gcagttggtt tatgatggtc gatatctgct
     3901 tgttttgttt cttgaacaaa tttttcttcc actatattca ttgatttacg cctcttttac
     3961 ttttgctgat tggacttcat aacctaattt ttccacaact gcagctaatt gttcagggtg
     4021 gacttcatct ggatcaaagt ttgctttgat tttgctcgca ttaaacaaaa ctttcgtatt
     4081 gcttacccca ggctgttgag aaaccgcatg ttcgattttt tgcatacagc ttggacaagc
     4141 tagtgtatct aattgcataa taattttttt catcgtagtt cttccttcct ttttcttgat
     4201 agtcttattc tagattacag cttccataaa aaaattgatt cttatcaagt tttacgattt
     4261 ttcttctttg ctttcgtgtc caaaatacaa taaacgcatc gcgttaaaga taacgatcaa
     4321 gatactagct tcgtgaacga acatcccact agccatgtaa ataaacccgg caaataagcc
     4381 gttgagtaaa aataagacga caccaatggc aatcactaca ttttcacggg tatttaaaac
     4441 agttttctta gccaagcggt aggcatgaac caacgattca aaattagatt gcatcaagac
     4501 aacgtcactc gtttcgatag caacatccgt tcctgatccc atagcaatcc cgatatcagc
     4561 cgcagccaaa gagggagaat cattgattcc atctccaata aaagcaacgc gcaatccttg
     4621 ttcttggtat tttttcacaa attgaacctt ttcgtctggt aataattcag catggacttc
     4681 atcaatgcct aacatatcag ctacataatc agcagtcatc tgattatcac ctgtcagcat
     4741 cactaactgt tttgctcctg cttgtttcaa cttagctaat tgttcagcga cttctggtcg
     4801 aatcacatct gacacaccta tgatttgagt gatttttttg tcgattgcta caataatgac
     4861 cgagctgcct gtttgttgta attgatgtaa atcttgtact tgctttggtg tgagagaaag
     4921 tttgtgagcg gcaatcaatt tggtgttgcc cgcacaaact gtatgaccat caatctcagc
     4981 tgtgattcct tgtcccttga ttgtctgatt atctttgact gctaattgtt gaaaatcaat
     5041 gtctttctcc tgagcataat ggatcacggc gcgccccaga ggatgatcgg ataacgtttc
     5101 taatcgagcc actaaagcta agctgtcccc ttcatcctct gtatagttct tgatagtgga
     5161 tacagcagta gttcctttgg tcaatgtacc agttttatca aagacaaaag tatcgatttt
     5221 ggaaaaagta tccattgctt caccgccttt gactaagatg ccccgtttcg ctccattacc
     5281 gattcctgct acattcgaaa caggcgcacc aatgactaaa gcgccaggac aaccaagaac
     5341 caaaacagtg attgctaaac gaaaatcacg actaatcaga ccaacaacca acgcaataag
     5401 taaaacggtt ggagtataat actgagaaaa tcgatcaatg aatttttccg catgggattt
     5461 ggtatcttgt gcttcttcta ctaattcgat aattttggca aaggtcgtat catctccgac
     5521 cttagttgct tcaatcgtca cgtagccatc tgctaaaatc gttccagaga agacttcatc
     5581 tccactgatt ttgacgactt gtcgtgattc accagtcact gcggcttcat ccgcataccc
     5641 tttcccgtca atgactcgtc cgtccacagg aatagctcca ccagttttta ctaagacgat
     5701 atcgccttct tccacgtcat cgatttccac ttcttcagtc gttccatctt cttggacaac
     5761 actagcagta gatggggcca tctcggtcaa agccttgatt gactcccgcg ttttggtcaa
     5821 tgttttttgt tccagatagc taccaaacaa aaacaagaat gtaacgatag ctgactcctt
     5881 gtattctccg atcataaagg caccaagcac agcaatagta acgagtaaat caattgagat
     5941 aactttgact ttcactgcct gatacgcatg gatcataata ggtatgaccg caataataga
     6001 agctatggct agagcaatgt tatagaacaa gacaaaacca gtaaattttc cgataaaacc
     6061 aaaaataatc aacacagctg ataaggcggt gatatgattt cgatgctctt ggatccactt
     6121 ttgtaatttc atctttctcc ctcttttcct ttcattctta cgagactaag tatagatact
     6181 tatccatcat aaaaaattga ccactatcaa gtttagtcaa aaaggcggtt ttcttttggt
     6241 agaatacact acaaaaaata gttagcaaga agactcacta aaagtgatct tgccaactat
     6301 tgtcgtttct atcgaaattt tcattgaatg attttcctgt acagcataca tcggagatct
     6361 atttctttat ctaagatttt tatcttgcta attttttcct ttagtctttc aaatccacgt
     6421 cgttcataga attgatacgt acagccacta tctgtaaaaa ggaatatttc ttttcctttc
     6481 tctctttttt caaattctgt tagcaacttg gtaccgatgc cttttgtttt caaatcagga
     6541 tttgcagcta aaaaaatgat ctctccatct ggcgaatgct tctttaaata tgctgcaagc
     6601 atttgttcat ttgctgtctc gtaagctcct ttactttctt tagcaaatat attttgtaaa
     6661 aaatcgaata tcttaacata tatttgctgt ccaaaagaac gatgtttctt tttttctcct
     6721 tttatctccg ccagcaaaac acctgccaac tcgtcctctg catacaaagc aagaatttga
     6781 gttgctcgat tcaactccat atacaaaaaa tatcttccgt aaagattcag cagaagttca
     6841 ttttccatat atctctcaaa gttcattcct tcaacagcaa aatagatagc ttttttgtaa
     6901 tctttctttt ctaagggttt aaaagttagt ttcatcattt cgcctcctca acttcaatga
     6961 ttgtgttata cttacattat aatagacaca gtgtctacta tctataatca atcatctata
     7021 agtttgtcga ttaagcgaca gctttgcttt gatatgtcgg ataaggagat aggtaaatga
     7081 aaaagacaga tagacgcgtc aaaaaaacag aaaaagcttt ggctgaaaca ctatccacat
     7141 tattagtaaa taaaaagatt caagcaatca ctatacgcga actaacagaa acggcagatg
     7201 tacatcgttc gactttttat acacattata aggatatcta tgatctatat gatcaattag
     7261 aaagcaactt ttttcgggat ctaaatgaaa ttctctctta tgatccagcc catagctatg
     7321 aggaactata cactcgcttg attgactatg tttacgcaaa tactttttta tatcgcttat
     7381 ttatcctcaa ccatggtgat atttcgtttc agaagaaagt taaacaagtt attgaagaaa
     7441 actatctgaa catatggcta tatgaagaca agaaaacgat tatcacagaa gaaatgagat
     7501 tctttactac gtaccacgtc caaggttgtc tatccgttct caatcggtgg gtagaaagca
     7561 attacaagta ctccaaaaaa gaattattag aactcttaca gaaactgaat gatcacatag
     7621 agaaaatcat gccttgaata aaaacatact aagcttagtt aagaagaacc gtaaccttct
     7681 cgtataacaa ctgaatctta tttagccaaa ttgtttactg aagatttttt gagtaaaatt
     7741 tctataattc ctacttattg aaaagtagtt tgtagtgctg cttctccctc tctaagtgat
     7801 atatcttgtt gtaccaatca aattcacctc atcattaata aattactttt aggctaattt
     7861 attacaactt ataggttgaa gctgtaacca atttatagtc atattatatt tttatcatcc
     7921 agtttattga attcttctcg tttacctcaa ttcattttca aggggaatca gctcatcatc
     7981 taacatttta cgccatgctc gcttcatttc ttgtgggtat ttaaatggtt tcgcttctgg
     8041 atgtttggct ttatatcctt cgtaatcctt actgttgata attgctttca tattaggaat
     8101 tggatcttca cctggcatat attgcagatt agaagcatgt aattttttct cagaaacaaa
     8161 ccactcattg gaaaagacat gaatcaaatc atcacttact tgtgtaaaat agcgacgttt
     8221 aatgacaaag atatcttcat tgctatctag tgtaccttca tttctttgtg cttaaattca
     8281 aagataattt tctcaataaa gatttttgat tccagtaaga ttcctttact ccttttacaa
     8341 catagcgttg
//
LOCUS       DACDEG010000102         8228 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.107, whole genome shotgun sequence.
ACCESSION   DACDEG010000102 DACDEG010000000
VERSION     DACDEG010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.107"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..391
                     /locus_tag="IV142_002381"
     CDS             <1..391
                     /locus_tag="IV142_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP8485982.1"
                     /translation="YNLKKFNHPLVFESDSSENHYNGKYYHQHHQVHEFKSILLTSDQ
                     DIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQDFIVAMQEVNEQVNFQYTTRQKDQ
                     TTIILIGKMLDEERIKQSFDKLLAESK"
     gene            421..543
                     /locus_tag="IV142_002382"
     CDS             421..543
                     /locus_tag="IV142_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP8485983.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            561..2084
                     /locus_tag="IV142_002383"
     CDS             561..2084
                     /locus_tag="IV142_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP8485984.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            2108..2347
                     /locus_tag="IV142_002384"
     CDS             2108..2347
                     /locus_tag="IV142_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485985.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(2540..3001)
                     /locus_tag="IV142_002385"
     CDS             complement(2540..3001)
                     /locus_tag="IV142_002385"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8485986.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(3196..3414)
                     /locus_tag="IV142_002386"
     CDS             complement(3196..3414)
                     /locus_tag="IV142_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8485987.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            3532..5148
                     /locus_tag="IV142_002387"
     CDS             3532..5148
                     /locus_tag="IV142_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP8485988.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(5389..6729)
                     /locus_tag="IV142_002388"
                     /pseudo
     CDS             complement(5389..6729)
                     /locus_tag="IV142_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(7005..7160)
                     /locus_tag="IV142_002389"
     CDS             complement(7005..7160)
                     /locus_tag="IV142_002389"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP8485989.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(7456..7788)
                     /locus_tag="IV142_002390"
     CDS             complement(7456..7788)
                     /locus_tag="IV142_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8485990.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
BASE COUNT         2675 a         1325 c         1618 g         2610 t
ORIGIN      
        1 ttataatttg aaaaaattta atcatccctt agtttttgaa agtgattcat cagaaaatca
       61 ctataatggc aaatactatc atcaacacca tcaagtacat gagtttaaat caattctctt
      121 aacttcagat caagacataa aaaaagattt tttactccaa tggattgact ggctgatttt
      181 aaccaaccaa gggagcctgt atcgttttaa aggattgatt gctttacaag agcaggattt
      241 tatagttgcc atgcaagagg taaacgaaca ggttaatttt caatacacca ctcgacaaaa
      301 agaccaaacc actattattc taatcggaaa aatgttggat gaagaaagga tcaaacagag
      361 ttttgataaa ttactggcgg aaagcaaata atgataagta gtgattgggg gtaatttcta
      421 atggagaaaa cggatatatc tagtgcatat cgacgactta aaagcccgaa tattaaaact
      481 agaaaaaggg cattaaaaat tatcaaagaa cataaacgaa ataaaatgaa gaaattagca
      541 taattaagga aggaaaaaga atgaagaaaa tcagtgtagg acttattggg atagcagctc
      601 taggattatt gggagcttgc tccagcacta atgatgcaaa agtatcgaat gataaagatg
      661 gaaaattaga aattgtaaca acattctatc caatgtatga tttcaccaaa aacatcgtcg
      721 gcgatgaagc gaacgtggat ttaatggttc cagcgggatc agaacctcat gactatgagc
      781 cctcagctaa ggatatggca aaagctcatg atgctgatgt ttttgtatat cataacgaaa
      841 acatggaatc atgggtacca aaagctaaag aaagttggaa gaaagctggg cctaatgtag
      901 tagaaggaac aaaagacatg attttattgc caggtagtga agaagaagat catgatcacg
      961 gagaagaaga tcaccatcat gaactagatc ctcatacgtg ggtttcaccc aaaatggcaa
     1021 ttaaggaagt ttcaaatatc aaggatcagt tagttaaatt gtatccgaaa aaagcaaaag
     1081 tatttgagac aaatgctgaa aagtatttga ctaaactgaa acggttggat gctgactata
     1141 ccactagtct taaagaagcg aaacaaaaaa gttttgtgac gcaacacgca gcctttggat
     1201 atttagcttt ggattatgga ttgaaacaag ttcctattgc tggactaagt cctgaagaag
     1261 agccatcttc tggtcgatta gctgaattaa aagagtatgt taaaaaaaat aagatcaatt
     1321 atatttactt tgaaaagaat gctaatgata aaattgcaaa aactttggcc aacgaagctg
     1381 ggataaaatt ggaagtactg aatcctcttg aaagtcttac aaaagaacaa atggataatg
     1441 gtgaagatta tgtttcagta atggaagaca atctaaaagc gttagaaaaa acaactatgg
     1501 ttgctggtga agaggtagtt ccagaaaaag aagcgaagga tgaaaagaca gttgctaatg
     1561 ggtactttaa agatgccgat gtgaaggatc gcgaattgag tgattataca ggcgaatggc
     1621 aatctgttta tccgttgtta aaagatggaa ttttggatga agttttcgat tacaaagcaa
     1681 aaatcaataa agacatgaca gctgccgagt acaaagatta ttatactact ggctataaga
     1741 cagatattga tactatcaac attaaagaca acaccattga ttttgtagtc aacggtgaac
     1801 atcatcaata cacatacaaa tatgttggat acaaaatttt gaattatgaa aaaggaaacc
     1861 gtggtgttcg ttttaatttt gaaactgatg atgcaggtgc tggtcgtttt aaatatattc
     1921 aatttagtga tcatggaatt gcaccaagta aggcagagca tttccatatt ttctttggtg
     1981 gagaaagtca agaaaaactc tataatgaat tgcataattg gcctactttc tatccagctt
     2041 cattgtctaa acatgaaatt gctcaagaaa tgatggctca ttaaaattca atttttggag
     2101 gaaatacatg gtaagaaaag tattattgtc tatggattcc aagaatattg atgcttatcg
     2161 gcgttatcga tttgcaaagc aaatgagatt aaatgggagt acattagctg atattgctgc
     2221 aactttacat gttagtattg acacagttca tagagtatta aaacgggatc ctgattgttg
     2281 ttttatcaag gtcaatactg gaagaagtag acgtttagca aaacggcata atagcttggc
     2341 caattaattg ataataccag agtagatcat ttttccctta tttggatttg atctgctttt
     2401 tattatgtct tgaattgtca aatcaagcgc aacactttat caaagaagac ttcataaggt
     2461 gttctccaat ctaaacactt tctaggtctg ttattcagtt tttcaatcat ctgatttatc
     2521 gttgagttat ctaccgtagc tatctctttc gattttggca agtattctcg aatgagtcta
     2581 ttcgtatttt aatttgttca ttgttgccaa gggcatgagg atctggaaaa taaaagggca
     2641 tattattcaa tgcttctgta actgcgaaat gattggagaa ttcttttcct ctatctggag
     2701 tgatcgtttt gaccgcctca tttggaatac tgcttaaaag ttcaatcatt ttctcgctca
     2761 cataagcggc tttctttctg gggaccttct ttcctgctaa aaggaagcat gattttctgt
     2821 ctgtcaaagt cactaaacaa gtaccaccag ttttgccagc tacaatatcg gctttccaat
     2881 gaccaaaatc acttctatca tctgcggatt ttggacggcc atggatcgaa tgagtaaggc
     2941 aaatctttcc acgagactcg acatgatttt ttgtatgtct tgtttttcct cgatggtgca
     3001 atgaacggat caggccttta ttgccgtgtg agaggggttc tgtatcgaaa agatcattgc
     3061 aaattgcacg ttagatcgtg ataaaactaa ttgaaaagtc catatgttca tgcttcaaac
     3121 gattcgagat ttcctctggc gaccactgaa aatcgacaaa taaatgacga acaattgacc
     3181 aaactcacgg atcatttaga atgtgataac gtccgcattt ttttcttcga taagcataat
     3241 ttttttccgc tttggaaggt gaatagtttt tttcttgttt cttctcagtt catgtaaaat
     3301 agtaaacggg ctatgctgaa tgtcgttgcc aattatacta atagaaaatc cttcttcgtg
     3361 aagcagaaag attctttctc gttcgagtat agtaagatga cgatactggc tcataattat
     3421 tttccttcta ggagtgttct cgacaaactt atactagttg gatatcgatt atgactcatt
     3481 tttatgtgtt gcacttaaag tgtaaattca aggtatagaa agaggttccc tatgaaaaaa
     3541 atacctatta caatcttaac tggctatctt ggttcaggaa aaacgatact tctaaatcat
     3601 attctgtacg gtgatcacgg aaaggaaatt gctgtcattg taaatgacat aggggaagta
     3661 aatgttgatg gggtattagt cgacaagaaa ggttttaaaa gaactggaga aaaacttgtt
     3721 tcgatgccga atggatgcat ttgttgtaca ttacgcgaag atttgattgt tgaactagat
     3781 caattagtgc aattttctga cttgaatcaa attgttattg aagcttcagg aatcagcaaa
     3841 ccaattccaa ttattcaagc aattatatta gctaaaaacc ctttagacat tgatttgacc
     3901 caacgattag agattggtgc cattattata gtagtggatg taaaacgttt ttgggataat
     3961 ttcgaaacgg gtgaaaagat tcaggaaaga aaagctgaag gtaatgtagt tgaagaagaa
     4021 caggatattc gtggcttgtt aattgatcaa ttcgagtgtt gtaacatatt acttttaaat
     4081 aatagtgact tagtgtcaac caagacgatt tacaaaatta acggtctaat tgctaagtta
     4141 cagtccaatg ccaaaataat taggacaatt aaaggtgaag tagacttatc gaaaattcta
     4201 aatacaaatc tttttaatat agatgagact tcctattcag tgggctggtt caaagagtta
     4261 gagcttggct atcaaaatca tattcctgaa acagaagaat atggtatctc aagttttatc
     4321 tatcgaaatc ggttactcct tgacgcacaa aaatttgccc atcttacgga taaaaatttt
     4381 cctggcaata ttacaagagc aaaagggttt ctttggtttg cccaacatag tgatttttcg
     4441 acgatctttg aaacatcggg tagctataaa agtattgaat cattaaatta ttgggtagct
     4501 agtttaccta aaattgagtg tcaaatgatt atacaaaaaa atcccgaatt gaaggaagcc
     4561 tggcatccgt tgtttggtga acgcattaat aaactagttt ttattggtat gaagatggat
     4621 caacaatgta ttaaggaaca acttgagggt tgtttaactg attctcttaa tgcagataat
     4681 ttccgaaaaa tttcaccatt tccatggaaa aagtctaata tgtttagcca gatatcgtta
     4741 atgaaatttt taagaaaagc tggtgtcaag ataggcgatg tcataattat tggcattttg
     4801 attattacct cttttattcc cttatttgta ttctctatag aaaaggagaa tgaaacaact
     4861 gaagatgcta tcaaatatgc catcgttcga attgatggca aggaaatgga tcgcttcgat
     4921 ttggataaga tcaatcataa actcgtgaat tatcatcctg cgaagggaca atataatatt
     4981 gttgagatta aagatggaag aattcgagtt aaagaagata atagtcctga tcaaatcgcg
     5041 gttagaactg gatggatctc aaaacccggt caaacgagta tttgtttacc ccataagtta
     5101 gttattagta ttgagaaaaa agaaagcaca gattactaca tttactaatc tggaaataga
     5161 tgaggaggtt tttaataaga aaaagatatt attattaata atagcagggg ctttattaag
     5221 tgtgttaagc gcttgtggac aagaaaaaga agcggtgaca acaggcacaa gtatctttaa
     5281 cgggacctgc aatggtttag ttaacagtcg gttagattac ttattttggc tcttcgtcaa
     5341 tgagtgttgg tgaagaaatt caatagaata atcgagatac tcacacggct agacagctat
     5401 cttgtctagc cgtgttgttg ttctggcttt agtcagttgg gtagtattct tagttttctt
     5461 aaaatatttt cctcgatata aaaagattcg ttctcggtag ttttgaaagt ttctatagcc
     5521 ataggcaatt cgttttagta ctttgatatg gttattcaaa cattctatag ggccgttgga
     5581 atagggaacg cgcaacgctc gtttgatttc tgtttggtat ttcttgaagg tcgtgaacac
     5641 tggttggtag tgctctggca actccttgta gtcttctttt aggagcgcga cgaattcagg
     5701 aacatcttgt cttctgataa cggaaagaaa atcctggtag acttcgtagc ctcgctttaa
     5761 ggaatcatca taagataata gccgatcgac tatctctgct tctgtcagat gatctcgaaa
     5821 tgacggacgc cagtagcgat gctgaccttt caattcccac gcattctttt gaagtagttt
     5881 ccagtatttc tttaagtgct tcgaccgacg atcccctttt ttaaaggtct tcattacttg
     5941 aattcggtgg ttggtaaaag cccgaccaat gtgttggcta atatgaaagc gatcggcgac
     6001 gattcgggca ttcggaaaac actctttcac caatgtgcga tagggtgtat agaaatctga
     6061 aaccactaga cgcacttgtt cacggacttt tatagggtag cgcaagaaat agtcgcgtaa
     6121 aggattgagt tgccgatttt ccacaatatc aatcagttga tgggtcttgc cgtccatcat
     6181 gacaaagctc atggctcccg agacattttt cactgatttg aattcatcga aacacaccac
     6241 ttctggtaac agctggctca aagaaatggt tttaggccga aggctgcgaa gtattcgaat
     6301 gacagtggtc ggagaaatgt gcttcatacg tgcgatcaaa gacatagaaa gaggttcgct
     6361 taatagctca agaatggctt gtttgactct gcgagcaatg gagcagcgac gatcactaac
     6421 tgaatcttct gctaaaaaag tctttccaca gtgtttacat ttgaagcgct gtttgttaat
     6481 ccgcagaatg gtcttgtagt tactgacatc attcaataag atggatactt ttttccagcc
     6541 ctatttaatg atcgtttgtt cattttgata gtggcgatga aagcattcct ccggttgata
     6601 ggtcaatgtg ccatcaaaaa caaaacatgt ttcgtcattg attcgctctt tgcgaaaggc
     6661 atcttcatga aagataagat ttaggtctag aatatctagt aactctttgg taaactctga
     6721 ataggacatc ttgaaacact cctttgaatg gttttcgtcg actttattct aaaggatttt
     6781 aaggtgtctg tttgcgttta ttagaaaaaa gaaaaagtat tggcaacaga aggcgtcacc
     6841 aacactagat attatagatc ccttattttc ttcgcttcaa attgtcaagg agtcatcata
     6901 aattttgtat ggattctttt gtttgggtag tagtcttcta aataatcttg gattaacacc
     6961 ttcatctcta ttttactttt atatttgttg gagtcgatta attctcattt gattgatcga
     7021 tgaaacgctt caattactgc attgtcaaca ggagtcccag tttggctcac tgaatgtgtg
     7081 atgtggtggt cgttcaacag ctcttgataa ataaaagacc gatgctggct acctctatcg
     7141 gaatgaatta ccgtaggcaa ttctccttaa taaatttgta atggacggac gacaatctct
     7201 tttttcatgt gtgaatctac catatagtaa cgtagaatac gagttgctaa gtcaatatag
     7261 gtggacaaat agaggcgacc gtcattacat ggaatgtaag taatatccat cacccaaaca
     7321 gcatcaattt ggttctgttc aaattatcga ttcaacattt ttttaccatt tctttgattg
     7381 ttttttctct accatgagaa gatttgcgtc gatgaaatcc tcttgtgcat aaattcattt
     7441 cacgcataag aacagctaca acacgtttat tgatttcgat tccttcatcg taaagttttt
     7501 gagtgatccc tggagatccg tatcgatctt tgtgttcgta gaacactcgt cttcctcttt
     7561 tagtcgttta ttttcctgtt caagaatgta gaaatctgct ttatcaggta atctttttcc
     7621 ttgtccagca aaagcatttt tcccgtatcg acgatattct gaaacccatc gggaaattgt
     7681 tggacgagag atattgtatt cttcagcaat tttcttggta gttacttttt tctcaaggat
     7741 atcctgtgaa atctttagtt taaatttacg atcatatgtt tttctcaata gagttcattc
     7801 cttccggtaa cgtaactgat agtcaagaaa agtgtatcat agcccaactt agtatcaggg
     7861 tttgtcaact aaactgtgga agttgataga taagagtttt taacaaccaa aattctcttg
     7921 actatttttg aaatcagaca aattttgatt ggacacatag ttgaataaat ctaccgtcgt
     7981 tacggaaggt aaatcgcatg cttttcaatt ctagaggaga agtcctcgtt gaagtaccat
     8041 agcccagttc tggattcgac ctcctgccca cttcatacgt aaataaagta attttaggag
     8101 tgagactgtg acaaaagtta tttttaagct aaaaacgaat taaaaaacgt tgataattaa
     8161 aatcattcgt tttagattct atgaatcatt atagaacgct gtaaaatcga tactttataa
     8221 ttataaag
//
LOCUS       DACDEG010000103         8219 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.108, whole genome shotgun sequence.
ACCESSION   DACDEG010000103 DACDEG010000000
VERSION     DACDEG010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.108"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            318..1241
                     /gene="truB"
                     /locus_tag="IV142_002391"
     CDS             318..1241
                     /gene="truB"
                     /locus_tag="IV142_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP8485991.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1245..2186
                     /gene="ribF"
                     /locus_tag="IV142_002392"
     CDS             1245..2186
                     /gene="ribF"
                     /locus_tag="IV142_002392"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP8485992.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2581..3366
                     /locus_tag="IV142_002393"
     CDS             2581..3366
                     /locus_tag="IV142_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8485993.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3379..4230
                     /locus_tag="IV142_002394"
     CDS             3379..4230
                     /locus_tag="IV142_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP8485994.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4354..4806)
                     /locus_tag="IV142_002395"
     CDS             complement(4354..4806)
                     /locus_tag="IV142_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8485995.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5271..5639)
                     /locus_tag="IV142_002396"
     CDS             complement(5271..5639)
                     /locus_tag="IV142_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP8485996.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5789..6271
                     /locus_tag="IV142_002397"
     CDS             5789..6271
                     /locus_tag="IV142_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP8485997.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6308..7318
                     /locus_tag="IV142_002398"
     CDS             6308..7318
                     /locus_tag="IV142_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP8485998.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7433..8146)
                     /locus_tag="IV142_002399"
     CDS             complement(7433..8146)
                     /locus_tag="IV142_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8485999.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2779 a         1336 c         1666 g         2438 t
ORIGIN      
        1 gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc ttttttttcg
       61 tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc caatcaaatg
      121 gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa catgatttct
      181 atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa ccgttcatta
      241 tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt aaatcaaaaa
      301 tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc ggaatgacaa
      361 gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc ggacatgggg
      421 gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa gccacaaaag
      481 ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact ctaggtttca
      541 gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta gatatgtctt
      601 tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc acccagatac
      661 caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat gcccgcaata
      721 atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc cggacaagtg
      781 aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta gaatgcggga
      841 aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt taccctgcgc
      901 acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat gctctgacat
      961 tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg ctgccaatag
     1021 aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg cagaaagtaa
     1081 aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg tcagaagaaa
     1141 ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg aaagaaaaaa
     1201 acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat atcatcaaga
     1261 tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta gtactaggat
     1321 tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg gaaattgcac
     1381 aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg attgtcttta
     1441 aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa gaagaaaaaa
     1501 tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct tttgctcatt
     1561 tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa tatgctgttt
     1621 ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat tttcctgaat
     1681 atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa ggtgaaaaaa
     1741 tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa gcagcaaaac
     1801 tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca cgaggcagac
     1861 tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg cctaaagaag
     1921 gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg ggttcaattg
     1981 gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat attttggact
     2041 ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt ttaagagatc
     2101 aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat gaacaagcaa
     2161 cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt ttatgtataa
     2221 ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt gacaaaagtc
     2281 ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt cggaaataag
     2341 ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat ttcttgaagc
     2401 tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag caattcatga
     2461 aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta cactcacttt
     2521 aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg agtgggtact
     2581 atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa ggggattgga
     2641 acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa ctataactcg
     2701 gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg aaaagcagtc
     2761 tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga tacggcttta
     2821 gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa tcagcatcca
     2881 acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt gactggcgtt
     2941 tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa aggaaatatc
     3001 atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca ttacgctgct
     3061 tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc accaaaggga
     3121 atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc ggcaaaattt
     3181 gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg cgtaggtgaa
     3241 ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag ttatgttaca
     3301 ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca aggtggaaaa
     3361 ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt tgggtggggg
     3421 atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat attaggaact
     3481 ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc agctggtatt
     3541 tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat cggacaaaca
     3601 ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat ttcgactgca
     3661 ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg gtctggaagt
     3721 atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc tgctttaacg
     3781 gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag tctattagca
     3841 tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga tgcaagcgga
     3901 ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat ttatgtgatt
     3961 ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat agttggagcc
     4021 atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg ggttgctact
     4081 gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat gtacatcctg
     4141 catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt gctgatcgta
     4201 gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct catttattaa
     4261 aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct agaatttcca
     4321 acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa tatctgtctg
     4381 catcaataat ttttcattga tccattgact aaaatttacg acatgattga agttaccacc
     4441 gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac ggtctttttt
     4501 atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat tgattgctgt
     4561 aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca tgaatcgctg
     4621 catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa gattacgttc
     4681 agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat gaagataatt
     4741 tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg agtaaaaatc
     4801 tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat ttgcttaatc
     4861 acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt cagaaaattc
     4921 taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata tttaaatata
     4981 cctgaaaata atgggcagta ttatgaaaga tcactgatat tttctcgtat tttttcctta
     5041 ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt actaagtgga aaattcaaag
     5101 ccattcaatc agtgattttt ttcagcacaa gaaagactcc ttataaaaat agcagatact
     5161 ttgtcaatta agtgtttaac cagggatgat aaaaaggata ggattgaaca aaacctgtat
     5221 aagctgatga atagtttcct cgccagtaaa aggtttattc gtataaagct ttatcgccaa
     5281 aatttatcat gacctttttt atcttcaata ttaaatgaaa tcaattgttc aatcaattcc
     5341 cagttgatta cacttttcca aggaactttg atgacgttat ccgtatatac gtagcctgct
     5401 tcttggatat ttttttcaag ggcacgaatc gctgcttttt ccggtgcgat agaaaaatga
     5461 tttttagcaa aactaaatgc aataataaag gtaccatggt cagtaaacat aggttggttc
     5521 cattttacag ttgtgtctag ctggggatac gtttctgtta tcttagcaaa aaggtctttg
     5581 atcttttgtt gatgttcttt ttctttgatc tgattgatga tcggctgaaa tttttccatt
     5641 ctaaaacgcc tccattaagt ggtttgattt attttaccag aaaaacaata gttatgtata
     5701 aaaaacgaac aaaacacaag taatgttgta accgctatat ttatatgata agatgtctca
     5761 aataataaaa tagaaaagag acataaaaat gattgacttg gcaaagaaat tcttacacga
     5821 aaatatatat tttgagttgt atcatcatcg agccatttat acaaatgaag atgctttggt
     5881 aataaagaaa gaacaaggat ttacaggtac agagaccaaa agcttattct taaaagataa
     5941 aaaagataat aactacgtat atctgacttt tactacgaaa caaacggatt tcaaagcatt
     6001 agggaagatc gtcggtaaaa ggctttctat tgtttcatca gaacgcatgg aagaacaaac
     6061 gggacaaaag cagggcgctg tttctccgtt tggttatgaa aaacagattc cgattattat
     6121 tgatgaagag ctcttatcaa atgaaaaact tgtttttgca ccaggtagac cagatcaaac
     6181 gatggtcatc caaacggttg acttaaacaa aatcatccag ctacttgaga tgccgatatt
     6241 tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa atagtgcata cttggaggtg
     6301 aagcaacatg ataagttatc cattattaga aagaaatgca acgattgggg ttactgcacc
     6361 atcctcaggc gtatcagagg aacaccattc gttactcgaa tcagctttaa agagaatgga
     6421 agaaaaatct ttccaagtag ttactggaaa gacgctttgg acgcaagata aggaaaaatc
     6481 aggtccagca agaaaacgag cagatgaatt caatcaaatg atgcaagatg aaaatatcca
     6541 aatgatcttt ccgccttggg gaggagaatt attgattgag attctcgatt ttgtcgattt
     6601 tgatgctcta caaccaaaat ggatagtagg ttattcagat attagtacgt tgctacttgc
     6661 tgtcacatta aaaacaggga tagccactgc acatggaacc aaccttattg atttaagagg
     6721 agagtacttg gatgaaacga cagcaatgtg ggaaagtgta ctgaaaacaa gagaaggaga
     6781 atcgattcta cagcaacctt ctgaaaagta tcaaaaagag tggccccatg agcattcatc
     6841 atcttctgtt ttccatctca ctgaaccaac aaattggcaa tcagtcagcg gagatgcagt
     6901 aaggatgaag ggacgattat taggtggctg tattgatacg atcagtcatt taatcggtac
     6961 cccttatgga gatgtccaat cttttaaaag aaagtataat gatggaaagc cgatcttgtg
     7021 gtttctagaa aattgtgagc tatcgtcagt tgatttaaga agatcacttg ttcagatgaa
     7081 actggcagga tggtttgatg gcagctcggg gatcatgttc ggtagaagtg cagcagagga
     7141 tttgttagat tataaaatga aggatgtata tgaagatctg tcaaaggaat taaaactacc
     7201 gatcttgtat gatattgatt gtggtcatgt accgccacaa ctgacattga tcaatggtgc
     7261 ctatgcagaa gtagcggtag aagatagaaa agcgactgtt ttgcaaacgt ttatttaaaa
     7321 aattttttcc tgccttggag tttatcaaag accaacaaat ctaaatagaa aagaaaatga
     7381 aagaagctga cagtattttg ccagcttctt tttgtatgat ctattagaaa ttttaattat
     7441 ccataactat cacattgatc agctgtttca catcatttgg attacctgta ggaatagcta
     7501 ctacagatgc attatcaata atttcttgag ttgtttctct aatgaacgct ggataagatg
     7561 tttgatctgc aggcatttgc caatgtgaag gaactggata actagtgatt gtcccgtcat
     7621 ggttacttga atacactaaa actccctggt aactgtattt cccagtgagc ataaccacat
     7681 ctttcggata gactgcactc cctgcatcat aaggggcgat aggtgttcct gcaggataat
     7741 gaataacatg tagttcgaaa tcacccgatg tatctctaaa agtattacct ctaattgcta
     7801 gccaaacacg tgcgtactca atttgtagat cagaatatcc tgctaacgga tctattgact
     7861 gagaagactg tgaggaagtt gtgtctattg ttgaagaaga aatatttgtc tcaactgtac
     7921 tactagatgt agaagaagta ttgggctctg agactttact gcttgttgaa gatgtattcg
     7981 tttcagtaga agctgatttc tgagtacttg atttttcagc tgaactggtt tgtttgattg
     8041 aacttttgtc tgtggtcgca gatggatcac tattttcttt actaggagac gtacatccta
     8101 caaggaataa agagaccaac aataaaataa aacctaactt tttcatataa aatacctcat
     8161 tattttttta ataccagtat agcataatcc tgaatttttg aatccgtaaa accttgtct
//
LOCUS       DACDEG010000104         8085 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.109, whole genome shotgun sequence.
ACCESSION   DACDEG010000104 DACDEG010000000
VERSION     DACDEG010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.109"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(54..1689)
                     /locus_tag="IV142_002400"
                     /pseudo
     CDS             complement(54..1689)
                     /locus_tag="IV142_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2244..2807)
                     /locus_tag="IV142_002401"
     CDS             complement(2244..2807)
                     /locus_tag="IV142_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP8486000.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(2822..3865)
                     /locus_tag="IV142_002402"
     CDS             complement(2822..3865)
                     /locus_tag="IV142_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486001.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(3998..4402)
                     /locus_tag="IV142_002403"
     CDS             complement(3998..4402)
                     /locus_tag="IV142_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP8486002.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(4635..5270)
                     /locus_tag="IV142_002404"
     CDS             complement(4635..5270)
                     /locus_tag="IV142_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP8486003.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(5536..6588)
                     /locus_tag="IV142_002405"
     CDS             complement(5536..6588)
                     /locus_tag="IV142_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8486004.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(6601..7539)
                     /locus_tag="IV142_002406"
     CDS             complement(6601..7539)
                     /locus_tag="IV142_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP8486005.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
BASE COUNT         2607 a         1625 c         1280 g         2573 t
ORIGIN      
        1 ttattactta ctttttctta ttctctagta agctaatatt gaaaaaaatg taatcagatc
       61 attattttta tatgttgtaa cttcctgata agaaaactaa ttaaaagaaa gaggtaacta
      121 cataaactaa ttaaaaaagc taacttcata gttggaggat aaatggaaga aacttcaatg
      181 attgtttctc cttgtgttcc ttcaatcgac actagtccta ttatcaatct ctcttttttg
      241 tccatttact tttgcgactg ttccccaata atcaaataca ggaagcgtta ttttttgtcc
      301 tttagattca tgtactgtat agactatttt cgaatccatc acttgataag gcatagtatg
      361 ccattccttg ccaattctta cttcatgatt ctgcaaatgt tctttgtatt gtaatgcttc
      421 cgtgggtgca tagtcataaa gtcctccggg aaggagtgtc gtgtaacgaa taagattttc
      481 ttgattcatt accaagtgat ctctctgagt gacaacttta tgttcatagt ttgcttttgt
      541 tgcagcagat acagccaaca gtaataaaac aattgccgtt atattaacta cttttctttg
      601 cttagctgat gtgctcttca tgaaaacgaa cgataaaatc aacgagccaa acagtacttg
      661 aaagcctaag attcgccaag gaaattgaat catttgaatg ggtgtatttt gtaataaaga
      721 ccatggaaaa aatttggtgg ataagagcca agagactacc gcactaaggt aaatcacttt
      781 ttctttgcga ttaaatcctt taaaaaagaa gagtggacag atcaaagaaa taataccaat
      841 gattcctacc gtaaaggttg tcaggtcatt attcatcgca ccaatcaaac tatccgagac
      901 atttaatgct tggtgttgta aggtcgtttt atctggctca ttaatcgatt gaaccatgat
      961 ttgtcggaac atcggatacc aaaagtatgc tgttaaaaga atcgtcattc ctactgcctt
     1021 tgacaactcc acaatcaacc ggatgctttc tttttttctt gtttcactta ctacaaagcc
     1081 gcctaagact agtaagccaa cggctattac actcataaaa gtggaaagaa catgtgtata
     1141 aatgagtaag ctcatactga ctgctaatgg aattgcacat ggctttttct cataaatcag
     1201 tgcgtaagca taatacataa ctatcggtat aaccgtaagt gcaatcgctt cagcaatagc
     1261 agaacgatga taaatatcaa tcgtacggta cccataaaat gtatataaac agctaaatat
     1321 aaaagccgca tcgattcttt ttaaaaattt tagtccataa tgaaacatca gcatgatagt
     1381 acaaacattc aatagccaaa catataagat ataactaacg attaaattat tacttatcca
     1441 ataaaaaatg actgccggaa aaaaggtcaa gtagggataa aaataattca ctccgctccc
     1501 ataatgatta aacgtcgtaa aattgattgg accagaaaaa attgaagata accctttgac
     1561 tctagcaaaa tggaaggcaa tatcccccga tgtagaaata gtatgaacat ttctaaaata
     1621 attgattgaa taaagtccag caataattgc taggagaatg gacaatatta tttttttctt
     1681 tacttgcatt acttaattat atactccttt catggattgc tttatctagt ataacttaaa
     1741 ttttaaataa atcgttattt atttttactc tcacttaaaa aagcactaaa attataagag
     1801 agggaggtaa tggtctgaac accaagaatt gttccaacat cgaattgctt aactaatgag
     1861 ttcaaagctt aattatgtgg ctcttatatt taccgagcgt ggattgcgat ctctttagcg
     1921 aaacaaacat ctttcataaa aaaaacagcc atatccgctt tcttggatat gactatttac
     1981 tactacttta ctatctttca ttcttcttgt ctcacttatg ataaggcgct gcgtatcatg
     2041 agtgaatgat attaggaaaa cttcataaaa taaatctttt atacatttcc acttctagat
     2101 ggtcattcat gttagaagaa tcaaactatt tttttactgt cgtaataata ttaaaacact
     2161 agaacaaatc aattaaattc cataaaatat aaataataga cctagcatca tctggtataa
     2221 aatggatgct aggtccgtta tatttactca aaattgattc tacgaataga tcttcttatc
     2281 atttctattt tagtagtcat gcgttgttgt tcttctaaag gtggtaatgg tataagtagt
     2341 ttagataaat ttgtaccatt tacatttggt tgcccagttc cgtaagaaat tttttctaat
     2401 aacttccaat ataatggcga gtctaagaag cagtctacgt attctgataa taatttctca
     2461 actaacctaa ttcgaatcaa gtaagaagca aaaacagatt cctcacttat ttccttaacc
     2521 aagaaacttt ttcccatagt tcctcctgtc cttgctatca aaatgtcatt ttcttccaat
     2581 cttaaatcaa ctagtttaga attactaata tcacagtaag gcacactgga ccaatttaca
     2641 cgtccctctt gtatatctgt gattctcaaa aacttaacat tgccttgttt tttcgccgaa
     2701 tctgtatagc catattgaat cttagtagaa atacttccta aataaaccca tagtcgaaca
     2761 tcgctagatg atttttcttg tcgttcttta tgaacaacag tgatcatctt gagttgtccg
     2821 actatttttt agttgcagca atctttttgg gacgcttatt tgcatgaagc aacgttgcta
     2881 agtctagtaa taaaacattg gctaacacag gattggcttt ctcgcgatac gctgcggcgc
     2941 cactttcaaa caatactgct agacgatctt ctacaacaac gatttgttcc aaatacggtg
     3001 gaaatttgat tcgttttagg atccggctag gagagtcccg ctcggacact aagagttccg
     3061 aggtataagg accataagaa cgactggcta agagataatg gccataaaaa gccacacctt
     3121 gaatcttatc agatgttttt ctgctcattg tcacggttgg ttggattttg tctttgcggc
     3181 gaattgctgt tttcatttca gctatcgtcg gcaatagata gaaagtcaac tgtccttcac
     3241 cttttagtgt gaagttgcca gataccagtt gatcattgtt ttgagtcaaa tacgatgctt
     3301 gtggaatctc tgagagatca gttgtcagcc aataagcgat tggctgtccc gtctcttcag
     3361 atgtatctgc taagatatcg atcaaacgta aagcggctac tcgtccagta gcagatcctg
     3421 tgattgtcaa ccataaaagt tcccgttttg tatcatatac taagccccct acatgtggcc
     3481 gtttaggtaa aatcagcagt ttgatcggct gtcctgtttc tctatccagt acatgaataa
     3541 tagaatgatg ctgatggtca tagcagtagg cagaaataaa cacaaaatct tgagaaaaag
     3601 ccaacccttg cggcgtcatc cattgacaaa cctcattttc gccggtttcc ggcaccacca
     3661 ttcttgtttc tgttaatcca ggaatgttat attgttttgc ttcagccttt tcccaagctg
     3721 cttgaacaat aggataccgt tttaactttt tttcaatatc ctgatgtatc cgttgctgct
     3781 tccaatgctt aaactttagg acatagtaaa caagaaggcc aatgaccagc aacactaaaa
     3841 tcattattgc tacccgctgt gtcatttttt tgcctccttg ttctattcga tcaatcgtcc
     3901 atataccgct attaattacc gtaactacat gtttctttta ctagcgtttc tttataggat
     3961 ctttcataat ctcatttata gtaacattaa gaatccctta ttctactata gtcacttcca
     4021 catcaatatt gcctcttgtt gctttagagt atgggcaaaa tgcgtgggct tcttctgcga
     4081 ttttctctgc ttggtccttc tcaagtcctg gcaaagaaac ttctaaaacg accccgattt
     4141 ggtagccccc atcttctaat tgaaataaag atacccgtgc tttgacttca gaatcagact
     4201 caagattatt ttcttccagc atgtgttgaa ccgcgccgtt gaagcaagat gcgtatccag
     4261 ccgcaaaaag ttgttccgga tttgtccctt cactatggat tcctgggcgg tcgatcttca
     4321 tgtgcatact tccgtttggt gcctcaactt cgccatcacg gcctccacga ttgatcattg
     4381 ctgtttcgta catttttttc attgagatca tcctttcttt cgtcgttcta tattaagtgt
     4441 aacgagtatt ttttaatctg acaaaggtaa tgcttgcatt cggcgacatc agttagacaa
     4501 catcgatgat cagtgataga agcagtttat ctatacaaaa aaagaagggt tctatgatat
     4561 ttggtgtcct tactcgcctt gagtaacaac accaaatatc atagaaccac ttaatcagct
     4621 tcacactgct ttttctactt tcgtagttga aaaaaactta aacaaatcat cggcaaatag
     4681 ttaaagaaaa aacttaatcg ttgaaagaag cccaattgct taaagaaggc aaactgacta
     4741 atcgacggaa tccgtgctaa tccgtaaaga cctgcaatta gtaaactaat aactgttaaa
     4801 acaagataaa aatggctaag cgtaccttga ccactttgac gataaagcag aacaagtaac
     4861 aatgggaaaa gtagaaagcc tgcatagcct agcccagaac ctatattatg gatccaagtc
     4921 gatagattac aagagctttc attcgtattg atactgaata agccagtaaa aatacaatca
     4981 cctatcccat ataaaccaac tgttgcaaca acaagtattg caagtgtttt tgatgttcca
     5041 acaaataggt gatacaacgc aggcaacgaa agaacaaaaa aaagtccaga tacaactgac
     5101 cagaccaaga aagcacgtct gactggacta tctacgtcac caaacacact aattacggta
     5161 gtcatttgat tcagtttggg ataaaaaagg ccaaggatat acggtgtcga taaatcactc
     5221 atcacaccta ataataaaaa ataaaaacca tatttttgca aaaaaatcat tttcttcccc
     5281 tactttttag tgattattcg atacatccaa caatcaaatt ttttctcatt ttaacacata
     5341 tttttattct agttgtcttt tgaacaatca ataacataaa ggtgagtttc ttctacattt
     5401 taaaaaatcg aatggcactg aagagttcag ttgtcagcat caatctccta ctcaaaaagg
     5461 caaaaaaacg taagtcagat gttgttgaca tctcacttac gcatccattg attgaaaatt
     5521 tcttagtaaa atctactagc ctaaaaattt gaagacttct tctcgatcat acagtttaaa
     5581 tactttctcg cataaaaatt gagtaattag tgccgcaaaa acagggatga ctaaccaaca
     5641 aagaacaaca agaaagccat ttagccctgc atctaaagaa gccaacggac caacaagtcc
     5701 gaccacgccg aaaccagcag aagcaggcgt acctgaaata gaaaatagag cgacaggaat
     5761 agctgtaatc actgcactaa ctaaacaagg aataagaatc acaggttttc taaatagatt
     5821 aggcatcatc atcttcatag cgcctaaagc aatcgcaatt gtcacacctg acttattaac
     5881 cttccaagag ttcactacta aaacaaaagt ggttgcagct acgcccatcg cagccgcacc
     5941 agcagaaaca ccatctagtt ggattgccat tccgattgct actgttgaaa taggagagat
     6001 aatcaaaaag gcaaaggaac aaccaatcaa gatagacatt aacagcggtt gtaaggtagt
     6061 gaagttatta atcaatgatc caattgcaga agtaattgct gttacaaaag ggaataaata
     6121 aaaaccaagt aaacctgcac ctacacctac aacaatcggt gttaacacaa tcgacactga
     6181 cccaaaacta tttccaatca gtaaaatcag cccgacagca atcgatgctg tcagcatcgt
     6241 attaataata tcgcctgtac cagcaccaac gtaaacaccc gtttcaccca aatcaggtgt
     6301 gaatttgact acaccagaac caacaaaaga agctccggct acaaccatca ttctcatcgg
     6361 atcaaatcca aattgtaagg caatcaaacc accaatgatc aaaggtgttg ctagttgaaa
     6421 aataactgct aactgtgcga tcgttaccgc aaagggatac gcactaaaat attttaaaat
     6481 ggcacctaat actgcattag gaatcaatcc aataatgatt cctgttgctg taccagcgag
     6541 gactttattc agaaaaattt tcggtgatat tttttgtgaa gtttccattc aatctctcct
     6601 ttattttacg ttcaaactat cttcttttgc atggacaagc tcagtcaaga aatcacaata
     6661 aggcgtcgca acaccatatt tcttcccttt tctagaaatt gcgccattga tataatcgat
     6721 ttctgttaat cgatggttct taatcaagtc ttgatacatt gaaggataat gtaacccaat
     6781 tgtttccggg tcaaaacaag attcacaatg tgcaatgact tctgggacat ctagttcaat
     6841 cttctctact gctgctactt tggcaaactc attgacaatc gtcgccacca ttttatgagc
     6901 agtcgatgtt tttcctagct cagccatatt aacgtctaag attgtgcata gtccgttcat
     6961 tgttccgttc acacatgctt tgcgataaat agaatagtga atattgtcag aaaaatgagc
     7021 atttaatcct gattcagaca atttgtctgc taattttttt gcagctgctt cttttccatc
     7081 acctaaattt tgtaattcta ctgaaccact tccaaataat ttgacctgac caggaccttc
     7141 aagacctgcc gtccacatgg tatttccaat atagatattt tccattggta caaatttctc
     7201 aataatatct tcatgcccga taccatttaa tagacataag acctctgtat cttttttgat
     7261 taatgattgg atatcctgca gcattttttc cagctgcatc gctttggtaa atagaataat
     7321 cagatcaact tgatcttctt tttctacttc ggattgaaga acgattggta gttttgcttc
     7381 tacctcttta ccattaaaat ttgcttgtaa tccatgttct ttgatttgtt ggacatgttc
     7441 tgcccagcca tcgattaata atacttcgtt cccactttgg tgaagcatca acccaaagcg
     7501 acttcccatt gcgccagcgc ctgcaattgc tattttcatt gattcttcct cctttttctt
     7561 tctattatac tcatttgaga atcttttcac aacctcgttt tgttattctt ctcttattta
     7621 tcatgtttat atccaccatt caagttattt tatcagcaaa tcaataaaaa ctggaataga
     7681 atcccaattt catcgtaaga gaaactagac tagcaataag aaaatagaaa cattcataga
     7741 aaaaatctca ctcctctgat tataaaagag ggggcattaa cctcgtgtat actaaaacct
     7801 tccattcatt cgatggatga ttttcttttt gtgaaaaaat attcattgta gtacgatgtt
     7861 gcgcaacaca gacggatggt attgcttatt atttattaaa atcgaacaat acacttattt
     7921 aaaaacttct ttcccagatt ttcttccatt tttacagtta taactagtat tatcatgaaa
     7981 aatagccttt tattgtctat gggaataata atttaaaatt ggtacttttt tattaattag
     8041 ttcatcgccc aataatctct ccagtttctt agcatctcct gactc
//
LOCUS       DACDEG010000105         7804 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.110, whole genome shotgun sequence.
ACCESSION   DACDEG010000105 DACDEG010000000
VERSION     DACDEG010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.110"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(229..714)
                     /locus_tag="IV142_002407"
     CDS             complement(229..714)
                     /locus_tag="IV142_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP8486006.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            1194..1835
                     /locus_tag="IV142_002408"
     CDS             1194..1835
                     /locus_tag="IV142_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8486007.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1854..2489
                     /locus_tag="IV142_002409"
     CDS             1854..2489
                     /locus_tag="IV142_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8486008.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2507..3322
                     /locus_tag="IV142_002410"
     CDS             2507..3322
                     /locus_tag="IV142_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8486009.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3641..5077
                     /locus_tag="IV142_002411"
     CDS             3641..5077
                     /locus_tag="IV142_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8486010.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5266..7713
                     /locus_tag="IV142_002412"
     CDS             5266..7713
                     /locus_tag="IV142_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP8486011.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
BASE COUNT         2613 a         1314 c         1737 g         2140 t
ORIGIN      
        1 aataaaattc cgatttataa tcgaaaaaag aagcattttt atatatgctt cttttttcgt
       61 agaatcaata aatatggatt tggctaacta tggaagaata ctcgtattag ttttcagcgt
      121 tcatactagt tgttacattg tttgattggc caaaagaatt tctgatatat tttgtgctaa
      181 ataaagaaaa agacgcttga attgggaaat aagaaaacta ctctatcatt acaaattttc
      241 ataaactggc agatgttcta agtaaacaag tatcttttca aagtcggaaa aatccataat
      301 caacgtagtg gtattgatat ttggatgaaa gcctacggta tcttgacgat caatagcagc
      361 atcgatcacg acttggattt ttttctcttt gtcgtgcatc aatgctaatg gagtcactgt
      421 acctgctgga acgcctagga ggtcaggaag ctgttcttca gaaacaaaag ataagcgctt
      481 ttcttttaat tgttcagcta gttgttttaa atcggcgtgc ttttcatcag gcaagatgac
      541 aagatagatt tgttttcctt ttcttgattt caataataga ttttttactt gcggaccagg
      601 tagggaaaaa gggacatttt tgactgacgt aatcgcagga tgatcgactt tttgaaaaga
      661 gatacccagt ttttttagta gttcataagc ttcttgttcg gttgcacgac tcatcgcgca
      721 tcaccctcct ttttttatta gtgtatcata aaaaagagtt gacaaatgaa aaataatcat
      781 gtaaattaca cgtaaagtta aaacaaatga catcgaaaag aagagtagcc gttaggtgtt
      841 tttagagagt ctctggttgg tggaaagagg cagcagttaa ccgtgaaaca catcttggag
      901 tcgattttct gaagcgtata gtaggaaaat ccggggagcc cgttacagct gcggtcgttg
      961 ctagatgacc agataaggct ggcttttccg tgaggagtcg gtaaaaaaag gtggaaccac
     1021 gtgattaacg tcctttgatc ttcggatcaa aggacttttt gttttcatct gaacggcaac
     1081 gactgtgtga ggttttttct gcaaggaaaa aataaacaga ggtggaacca cgtataatac
     1141 gtcctcttgg catttgccaa gaggattttt ttattttaaa ggaggaaaag aagatggagt
     1201 atttgattga agttatgccg caattattag taggagcact cacaacattg aaattatttt
     1261 tatttacctt gattggctca ttaccattag ggattgtttt agcattagga ttgtctttgc
     1321 attttaagcc gctacgctac ttgcttcaaa catatgtatg ggttatgcgg ggaacaccgc
     1381 tgttgttgca actgattttt attttttatg ggttgccgac gattgggatc gtttttgatc
     1441 gtttcgaagc agcactcatt gcctttatct taaactatgc agcttatttt gcagagatct
     1501 ttcgtggagg gatacaatcg attcctcaag gtcaatatga agcagcgcaa gttttgcaat
     1561 taagtccctt tcaaacggtg acgaaaatcg tcatcccaca agtgacaaag attgttttac
     1621 cttctgtagg gaatgaagtt atcaacttgg tcaaagattc ttccttgatc tatatattag
     1681 gtttaggtga cgtaatgcgt gctgggaaaa tcgcgatgga acgcgacgcg actttactgc
     1741 ctttgctagg cgtggcaatc atctatctta cattgactgg tattctaaca atcgtaatga
     1801 aacaagttga aggaaaatta aattattacc gctaactgat aggaggaaga aatatgattg
     1861 aattgaagaa agtgaacaaa gcatttggtg aaaaaaaaat aatcaaagat ttgaatctaa
     1921 tcattccaga tgggaaaaca ttagctatcg taggtccttc cggtggcggt aaaaccacgc
     1981 ttttacgtat cttggctggc ttagaaagtt ctgacagcgg agaattttat ttagacggtg
     2041 taccgttcaa cccattcgag cttaaaatga aagaacaagt tgtaggagtc gtattccaag
     2101 acttccagct atttccacat ctgaatattt ttgaaaacat taccttagca ccaaaaatgg
     2161 tattgaaaca gacaaaagaa gaatacacca aaaaagcaga agaattagcg gcttcattag
     2221 ggattagtga tttattgaat atctatcctt atcagctttc tggcggacaa aaacagcggg
     2281 tagcgattgc cagagcattg gctatgaatc cacgggtatt ggcttatgat gaaccaacaa
     2341 gtgctttaga tcctgaatta cgccagcaag tcgagcgttt gatcctatca ttgaaagaag
     2401 atggggtaac ccaaattatt gtcacccatg atttagtatt tgcagaaaat gtcggtgatc
     2461 aattactaaa agtttcccca attcattaag aaaggagaag atagagatga aaaaaaccag
     2521 ttttctattc gctttgatcg caggtcttct tctttttgca ggctgttcga acaaaaaaac
     2581 ttcagcagat aattgggcga ctatcaacga agagaaaaaa atcgttatag gtttagacga
     2641 tacatttgtt cctatgggat ttagagataa agacgggaag ctcacaggat ttgatattga
     2701 tttagccaaa gcagtctttg aacaatatgg gattacggta gactttcagc ccattgactg
     2761 gtcaatgaag gaatttgaac tgaacaatgg aacgatcgat ttgatatgga acggctattc
     2821 caaaacgccg gcaagagaaa agaaagtgca atttacaaaa ccttatatgg aaaacgatca
     2881 agtgctgatc acacctaaag aatcaggaat cacaaacttt ggacaaatga ataataagag
     2941 attaggtgcg caaaatgggt catctggtta tgatgtattt acgaaacaac cagaagtatt
     3001 aaaagagata gtcaaaaacc aagatgctgt tttgtatgat agttttaatg aagcattgat
     3061 cgatctgaaa agcagacggg tcgatggtct gctgatggat aaagtatacg cagactatta
     3121 tttgaaacaa agaaacgaac tagaagactt caacataaca aaaggttctt atgagagtga
     3181 ggattttgca gttggtgcac gcaagtcaga tagtacatta gtgaaaaaaa tcaatgaagc
     3241 cctagatcaa ctagagaaaa caggtgaatt ccaaaaaata tccgataaat ggtttggcga
     3301 agatgttacg ccgagaaatt aacaaatagg ggactgtgac aaaagacgtg tcacagtccc
     3361 tttttccgaa taaacagtgt tctcctgcca actccttgtg actcggagct gaatggcagg
     3421 ctcacaacct cttggtaaac ggtgttcaaa agccagtttt tcggtattaa gccaaattta
     3481 tgcaaaaatg tagaaagcca ttttcacaaa atttttctta ctactcgaaa ctaagcgact
     3541 ttttcacaac ctcctttaca taattttatt ttgttgtgga aggggtttcg tgttattcta
     3601 aaagaggaac gattgaaggt tataggaaag gaagagatga atggaacaca aagaacttac
     3661 ctcatttcca aaagattttc tgtggggctc tgcttctgca gcgtatcaag tagaaggagc
     3721 ttggcaagaa gatgggaaag gagaatcagt ctgggatcga tttgtacgga tacctggaaa
     3781 gacatttaaa ggaacgaacg gtgatttggc agtagaccac tatcatcgtt ataaagaaga
     3841 tattgcttta atgaaagaac aaggtctgaa agcttaccgc ttctctgtag cttggacacg
     3901 gatttttcct aatggacgag gagaagtcaa tcaagctggg ctcgcttttt atgagagatt
     3961 gatcgacgaa ttgattgaaa acgatattga acctatgttg actttatatc attgggatct
     4021 tccacaagca ttgcaagatg aatataatgg ttgggaatca cgtcagatca ttgaagattt
     4081 cactcattat gcagaaacat tatttgaggc attccgcggg aaagtccatt actggattag
     4141 tttgaatgaa caaaatatct tcacttcatt aggttatttg ttagcagcac atccgccagg
     4201 tgtgacagac ccgaaacgaa tgtatgaagt taaccatatt gctaatttag caaatgcatc
     4261 tgtcatcaat aaatttcacg agttagaaat gcctggaaaa atcggaccaa gctttgcgta
     4321 tacgccaaat tatccaattg acagtaatcc tgaaaatgtt ctggctgctg aaaatgcaga
     4381 agacctgatg gccaattatt ggatggatgt ctatatgtgg ggaaaatatc cgattgctgc
     4441 catgaggttc ttagaagaaa aaggatgggc accaacgatt gaagctggcg atgcagaatt
     4501 gctagaatct gctaaaccag attttcttgg gatcaattac tatcaaacgg cgacgaatgc
     4561 attcaatcca ttaaacggag ttggtgcagg aaaaatgaat acgacaggaa aaaaaggttc
     4621 atcagaagaa actggagttc ctggaatgta taaaaaagtt gagaatccat ttgtcgaacg
     4681 aacaaattgg gactgggaga ttgacccaga aggattaagg atcggactgc gtcgtatcac
     4741 aagccgttac cgcatgccgg tactgattac ggaaaatggc ttaggagagt atgacaagtt
     4801 gacagaagat aaacaaatcc atgacgacta tcgaatcaat tacttacaaa gccacgtcaa
     4861 agccatcaaa gaagcaattt cggatggagc agaagtccta ggctattgca cctggtctta
     4921 tacagatttg cttagctggc tgaacggtta tcaaaagcga tatggatttg tatatgttga
     4981 tcaagacgaa acacaagaag gatctttgga acgatataag aaagacagtt attactggta
     5041 ccaaaaactg attgaagaaa atggagagaa cctttagttc acatacttaa aacaagaagg
     5101 tacggctgga tatggccgtg ccttttcttg ataaaatcat ttagaaatta agaaaaactg
     5161 taaaataagc tgaaaaatga aagaaagcgt cagttctttc aaacaaaact ttagttccct
     5221 tacttttttt tgtataatga aacttgatga aatgagggat aataaatggc acaaaagaaa
     5281 cggcctacta aaaaaaggaa aaagacgaaa aaaacgaaaa agcaaaaaca gcagcaggaa
     5341 caaatgctga cgattggtgt tgggtttgcc ttgattttat ttgctgtctt cggcttttta
     5401 aaattaggtt ttttaggaat attgattgca aacggctttc ggatcattgc aggaaatact
     5461 taccaaatcc tctgtctttt attagccgta ttaggatttt ggattgtcat taagaataca
     5521 gagttttcaa ttggtaaaaa ccgacgctgg tttggcggca tactctttta tttcggtatc
     5581 ttattattgt tacatgccca tttatttggc aaactccata ctggggaacc aaatatcatg
     5641 gggacgacat gggatttgtt agccagtgat ataaaacaaa gtcaagtaga caataacgtt
     5701 ggtggtggga tgattggagc aatcctttat cattttactt acttcttgat tgcacagcct
     5761 ggaagttatt tagcagcagt gctattactg gcaggaggag cattcctgtt cagtaatctg
     5821 gaaggctatc agctgttaaa tggtatccaa tcaattggag aacgagtaca ggaattattg
     5881 gaaggtgatc ctgccaagca agctcgaaag caagcggcaa aggaagagag aatgaaacag
     5941 cgcgcagaag cgaaagaagc aaggcgctta gctgcaaaag aagctgcaga aaaagaagct
     6001 gcagaatatg agaagaaaaa agcggtgagc cagcaaaggg aaaatactgc tgatgaatgg
     6061 caggaaccag aaaatacaga gccggagcag ctaagttttg ttccaatcga cagttttcaa
     6121 gaaaatatcc atcctgcgaa tctagagaaa ccagtaccag atacaccaaa acagaccaat
     6181 acagcagaag gtttttcaga tgagttgcca gaggatgacg gtacctcatt agaatttgag
     6241 attgaagcag aacaggaaaa tcaagactat gaattaccga cagttgattt gcttgattct
     6301 ataccaactg tggatcaaag tgatgagtat aaaaaaattg aaaaaaatat cggcgtatta
     6361 gaacaaactt tccaaagttt cggtgtcgat gccaaggtcg tcaaagccag cttaggacca
     6421 tccgtcacaa aattcgaagt ccagccggct gtaggggtaa aagtaagtaa aattgtgaac
     6481 ttaacagatg atatcgcgct tgctttagcg gcgaaagatg tccggatgga agcaccgatt
     6541 ccaggtaaat cactgattgg tattgaagta ccaaatggaa agatcagtat ggtttccttt
     6601 agagaaatca tcgaagcaca accaaaccac ccagataaat tactagaagt acctttagga
     6661 cgtgatgttt ctggacgggt acaaacggct gatctttcga agatgcctca cttactcgtg
     6721 gctggttcga ctggtagtgg gaagtcagta gcaattaatg ggattatcac aagtatatta
     6781 atgcgtgcta agccccatga agtaaaactg atgatgatcg atccgaaaat ggttgaattg
     6841 aatatgtaca acggcatacc tcatctgttg acgccggtcg tgacgaaccc tagaaaagca
     6901 gcgcaagcct tacaaaaagt agtacaagaa atggaagaac gatatgaaaa attcgcggca
     6961 accggagtaa gaaatatttc tggttacaat gaatttgttc aacagaaaaa cttggaaaac
     7021 ggaacgaaac atccaacctt accattcatc gtagtgatcg tagatgaatt agctgatttg
     7081 atgatggtag ccagcaatga agttgaagat gcgattattc gtttagcgca aatggctcgg
     7141 gcagcgggga ttcatatgat cctagctact caacgtccaa gtgtggatgt tatcacagga
     7201 atcatcaaag caaatgtacc ttccagaatg gcatttgcgg tatcaagcgg tacagattct
     7261 cggacgatca ttgattctaa tggggcagaa aagctcttag ggcgaggaga tatgctattc
     7321 ttgccaatgg gtgagaataa accgatccgt gtgcaaggag cattcatttc tgaccacgaa
     7381 gtagaacggg tcgttcaatt tgttacagat cagcaagaag ctcattatga agagaaaatg
     7441 atgccgacag atgaagttga aacgacaggt gctccagaac agccacaaga tgagctgttt
     7501 gaagaagcaa aagcacttgt cgttgaaatg caaacggcaa gtatttccct tctccaaaga
     7561 agatttcgga tcggttacaa ccgagcagca cgtttagtag atgaactgga agctcatgga
     7621 gttgttggcc cgtcagaagg aagcaagcct cggaaagtat tcattgagca ggaagaaaca
     7681 tcagaaacgt tgccggacga taatgacatg taaaacttgt gtgatgaagg gttgaaaaac
     7741 gaataaccaa taaaaagaca aggcatgatg ataccaaaag aaaaaatggt ttttttccat
     7801 cttt
//
LOCUS       DACDEG010000106         7797 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.111, whole genome shotgun sequence.
ACCESSION   DACDEG010000106 DACDEG010000000
VERSION     DACDEG010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.111"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            117..2444
                     /locus_tag="IV142_002413"
     CDS             117..2444
                     /locus_tag="IV142_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8486012.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2570..2899
                     /locus_tag="IV142_002414"
     CDS             2570..2899
                     /locus_tag="IV142_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP8486013.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            2939..3943
                     /locus_tag="IV142_002415"
     CDS             2939..3943
                     /locus_tag="IV142_002415"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8486014.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3940..5369
                     /locus_tag="IV142_002416"
                     /pseudo
     CDS             3940..5369
                     /locus_tag="IV142_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            5575..6747
                     /locus_tag="IV142_002417"
     CDS             5575..6747
                     /locus_tag="IV142_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8486015.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6909..7364)
                     /locus_tag="IV142_002418"
     CDS             complement(6909..7364)
                     /locus_tag="IV142_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486016.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
BASE COUNT         2650 a         1454 c         1539 g         2154 t
ORIGIN      
        1 cttagaataa aaagagaaaa aaagccccaa actttctata catcaataaa ataataaaaa
       61 ttaaatttag aataacgtgt aaaggattat ttgatgattt tttattgaga ggagggatga
      121 aaatgattaa gaaaactcaa aataaaatcc aacgtatatg tcaaaaacat tttgtttcta
      181 gactcaaagc tgtatctaaa agaaaatact tacatcccat tatgatttcg atcatcttga
      241 tttttacttt cttagggagc ttatcaagta tttatgtaaa cgtttataca gaaacgtcaa
      301 attttgctaa ccaaacgaca gaggagacaa atagtccacc ctattctgaa atacagtcca
      361 tagaaagcac caatcagtct agcaacaacg agacaacctc aaccacatcc tcgactcaac
      421 tgattgatcc gacagaaaaa gaaatcacag cagcaaaagc agcaggggag aaagcttatt
      481 cagaaacagg tagaagccag acatttagta ctgttgcggc cactgcgaat ttcaacggac
      541 ataccatacc agatggatat acagtgatat atgccaaaga tggagtcatt gttgcggtta
      601 acggatcgat gagtgcggat aaaactgcgt tacaaggcgt aaccaaagca atcgctttta
      661 ttgataaccg tccagatatc cataatctgt ttattggctc cacagtaaat ggtactacta
      721 tcacattccc aaaaagtatc tatatggggg tggctaccgc tggaaatacg atcgattcag
      781 ttacacccaa tgcggataaa agttattctt ttacaccctc aacatgggga gggcttcctg
      841 ccggaggagg tatgagtact ttttcaatcg gtatacagtt agcaaatgat attccattca
      901 cggggtactc gatcaaaaat gggcagatcg ttgatggtaa gggaaatgtt tcaaattttc
      961 caacctatca agcagcaacg gttcaggtca atgcgaatcg gacaggaact ttttcttata
     1021 cagtcaatgg tggagcagca aaaatcggaa cgtacgggac tacattatct ggaatccatg
     1081 tgggtgacaa agtggttatc actccaacag cagtaacggg ctattctttt gcacaaacaa
     1141 acggttcttt cacagcccta gaaagtaacg tcgatacggt gacctataca ggcaaacaaa
     1201 caccaatcac tattaagtat caagactatt ttggacagat gattgctcct tctaaaataa
     1261 tgaatcttac ttacggaagt gctgcacaag acctgacgac taatacgcct actatcaacg
     1321 gatatacctt tacagctgtt tcagcgacag aaacaaagaa ccaatcagca atgagtgttt
     1381 tagctagttt agatacaaat ggaaatgtaa tcgtaaagga cgcaaatgga aaacagattt
     1441 ctgaagtcat cctatattat aagacaaatg caacagtatc aatcaatgca aatggcagta
     1501 agtactatga tggtctttct gttgtaccga tcgttaaata tgcctttaat gataaaacag
     1561 aatcaacatc ccttttaaac aatgatctca gtattgatca aatcacatgg aatccaactg
     1621 atttcaaagc gatgaatgaa aagggagttg aagtaactgc gcctactgaa attggaacct
     1681 atacgtcttg gcaattgaca aaaagcggac tagctaaact agctgccaga acaaactatt
     1741 tatttactat cgtgcagaca tctgatgtct atacgattaa gcaaatcagc ggtgcggtag
     1801 agctcagtgg cagtaagaca tatgatggaa aagcaggtgt cccgtcaatc catgtcaagc
     1861 tagcagaggg tgtcactagt tcgcttactc cagtacaagt ggcacttagc agtatggatt
     1921 acacagcaga tgcacaatca gcaaaaaata tggttaatgc tggcagctat acaattaaac
     1981 taacgaatag cggaatagac aaagtcaaag cagcaaatcc caagttgtca tttactgata
     2041 tagccaacac atcaggaact tataccatct ataaagctaa cgcagtgatt actgtcgatg
     2101 acgctgcctt taattatgat gcccaaagtc actcgatttc ggttggaaat ctccacgtta
     2161 ccggtgttgt cttaggcgaa tcattggatt ataccctaac agttaacagc cggacagata
     2221 ttggatcaca aatagtaacg attagtttag gcgaaggaag tgtaaacaat aattatacga
     2281 ttatcgcaac agataccgct aagttgacga tcaatccgac tctcttgctt ccttcgacag
     2341 gtgggatagg tctactgccc tttgtctttt taggactgat ctttattttt tctggcttct
     2401 tttattttat ccatcgtaag aaaaaagcag gtgaacagcg atgaaaaaaa caaaaaatgt
     2461 acgtgtggat atatttgctg ttctcttttt tatcctactg gtctttttta taggttctcc
     2521 gactgtgtca gctagagaat cacctattac atttactttg cataatcgaa tgttcgaaag
     2581 cgataagatg ccaagtatca gtcaaaacga cggaataaat atcccggata actttcaagg
     2641 agtaaaaggg ttgaacggcg ttactttcgc tatatatgat gtctcggatg aattttacaa
     2701 gctgcggtca gaaggaagta gtgtggaaga cgcacaaaga aaactagcac aaaaaagtga
     2761 tagtgaaaaa atactggcag aaaatgtgac aaaaacagta gacgaggaag agggcattgc
     2821 ttcttttagt gcatcggaca aagatgagca aggtcgtgac gctgtttacc gatttgcgga
     2881 aataaagact tcagattaag taaaagaaaa atcagcacca tttgtcgttg tgcttcctgt
     2941 gacagattca tctaatcata aattgacgac catccacttg tatccaaaga gcgaacaaaa
     3001 aatacctgct gcactcacat taacaaaaac agtagagaat aagcagacag attttgcaga
     3061 tggggataaa gttccttatc tcatcaccgt aacaattcct gagaacatca acgagatagg
     3121 aacctatacg atcaaagata cagctgatcc tcagttatgg ctagaaccag aaacgatcga
     3181 ttttttgatt ggtggagaca agattcatac atttcacaca caaaaaacaa aacatggttt
     3241 tgtcttatca gattctggaa aagatcttag ttcgtttgct gggaaaaaat taacgatccg
     3301 ttaccaaatg acattgaaag aaaacagtga aaaaacgaca tttgataacg atgtacgttt
     3361 gacgactgac aatcaaacag tagaaacaca tcttgccatc caaacaggag gcaaacaatt
     3421 tgtcaaagtg gatcggcaaa cgaaacaaaa actagcagat gctcaatttc tagtcaaaaa
     3481 aaatgaccaa tacttagcgc aagaaaacgg gataaatcat tgggtagcca aagatgattc
     3541 aagagcaacg atttttactt ccagtaaaga tggaacattt tctgtccatg gattgtcttt
     3601 tggaagttac gaactagtgg agattaaacc gcctaaagga tatatattga gtcaatcaac
     3661 cattctcttt gaagttgaaa aaggcagtta tgatccgctt catcctatac cattggaaat
     3721 catcaacgaa ccaaaagcaa atccgacgaa taatggaaaa acgccagttc agatgcagac
     3781 aaatggtcca tcaaatgaaa aaaatggagg aagctttcca aaaacaagcg aagagatgct
     3841 tggaggtttc tcaattttag ggttgttgct agtcctttca acagggacag cttggtttta
     3901 taaaaaacaa caaaaaggaa acaataggag ggaaataaaa tgaaaaacat caaaacaaaa
     3961 atatggacgt ttttaggaac tgctatcatg ctgcttcctt tcgttctcgg cctaggaact
     4021 gcagaggttt ctaggccgta tcacctacac cagaaaatgt tacggttaat ttgcataaat
     4081 tgaaattcac ttcagcacca gaaaatcaaa taaacaacgg aacggaattg acatttccga
     4141 atagcgaacc gttgaacggc gttgaattca acgtatatga catcactgct acgtactatc
     4201 caagtaaaga cacagcagtt ccagcagacg ctacaccctt cgcaagtgta acgacttctg
     4261 gtgaaggatt agctaacctg actcttccag gtaaatctga tgggaaagat gctgtatacg
     4321 tctttgtaga aacaccaaag cccggtgtcg aaacgtcgcc aaatatcgtg ttaagcttac
     4381 ccttttataa cccgaattca tcagaccctg acaaaccttt agacacgatt cacctatatg
     4441 ctaaaaatgt catcaaagaa agcaatatca aaatccagaa agctgggaac ttttcagggg
     4501 tagataagtt agcaggggcg caattccaga tcaaaaattc agagaatcaa ttcgtgaccg
     4561 gttttgatga aaacactggt gtcgctctct atgatcctgc tgagtcagcg ttgacattca
     4621 caactgatac tgaaggatcc atcagtgtca atggattaat agacggcaat tatactttag
     4681 ttgaaacaaa agcaccagat ggttatcaat taaccaatcc acaaaaagaa actactttta
     4741 tagtaaaaaa cggaaaattg caacaaacaa ccggtatcat ccatggcgca gatgaagcca
     4801 atcttgggaa tccaaacggt gtagtcaatc gggaaaatac aactacggat aacacgatta
     4861 ctgtagaaaa tgtccaaaac tacggtgatt tccacttcat caaacaagat gtgaacacgc
     4921 aagaaaaatt agccgatgca gaattttcta ttctaagagc aaaagatcca actgttaaat
     4981 taatgaaaaa aatagcaaat cttgcctcag gagaataccc atatgcttgg tcagatgaag
     5041 tcacaggttc tgactgggaa gaagtgaaat tgatttctga ttcagaaggg aaattttcaa
     5101 tcgaagggct gaaacaaggg gcatactgcc ttcaagaaac aaaagcacca gaaggctacg
     5161 cattacctaa gaatgaagct gcgtacactg aatttacagt agatgatgat ccaacaactt
     5221 ctgatgaaac aacgatcgat aaccaaccta aaggaatctt gccttcaact ggtggtatgg
     5281 ggatcatcgt attcatcttg gtaggtactg ctttagttgg aggagcagtg atctacttca
     5341 aaaaacgcca tcaagaaaca gaagcttaaa ctattggaaa caaaaatcat acgataaata
     5401 aacgatagtg attcaaatgg ggagaaaggt tgtgacaagt ggtcggtctc ttggtattcg
     5461 ctcaacaaac ggcaaaatag gaggaactat tttgccgttt gttgagcgaa tactcaggtc
     5521 aaggcgctga tttcacaacc tcttcatata catattagaa aataggtgga catgatgaat
     5581 aaaagcagaa taagaatact ggacatcttc atggcaatca ttttagtcgt tgggatcggg
     5641 atcttttctt atccttttgt agaagattca ctgaatgatt ttcttgccca gcaaatgatc
     5701 atccattatc aaaagcaagc atcgaataag aattcagagg aaataaaaaa acagcaggaa
     5761 aaaatgacaa agaaaaacca acagttggca gagcaaaacg tatcaccagg gataggcagt
     5821 ttcaaccaag cagttgatgc aaaagcatta aaagatttac ctagtaatgc cttttttatg
     5881 gaacatatgc ttggagtcat cgaaatccca aaaatcaatg tcagcttacc tatattcgat
     5941 cagacaacgg aaatattttt gcaaaaagga acgtcattat tggaagaaag ttcttatccg
     6001 actggcggga aaaacaccca tgccgttctt tctgggcatc gtggactccc agaagcaaaa
     6061 ttatttacag atttgcctaa gttgaaaaaa ggagaccagt ttttcatcca gatcaatgga
     6121 aagacattgg cgtatcaagt agaaaaaatc caagtggtct tacctgatga agtagattct
     6181 ttgggtatcc aaaaaggcag agatctcgtg actttgctta cctgtactcc ttatatggtc
     6241 aatacacatc gactgcttgt gacggggcat cgtattcctt atcaagcaaa agaggcaaaa
     6301 aaagcgatcc aaggaattga tcaatggaaa aaatggaaat tttttatttg gtttatcggt
     6361 atactacttg gatcaatcgg cttagtttgg ctactcatcg cttatttaga ttctttggcc
     6421 atcgctaagc gaaactatcc tttatctttt tatgtaaaga acacaaacgg acgaccaatc
     6481 gaaggaatgg tcttttctgt aaaaacgctc aacggcaaac actatattac tagagaaaaa
     6541 gttccatttg tgaaagcttc tgatgaatac ggactagtga gattttctga tttaaaaggc
     6601 aggaactatc gcttgcaaca tgaggagctc ctgcttaaaa tccatgtaaa acataaacat
     6661 tcgaaacagt tttcaatgaa attgaaaaaa ggaagatata aactgcgaaa agaaaaagag
     6721 gtgtactatt tgattgagaa agaatgaatg aagatgtcca aaaaagcaga gggaacagac
     6781 taaatgaaaa aagagagaag ctgacgaaat ggacgtgtaa actgctattt ttggtttact
     6841 atctggaacg ccagcttcta tttttttatg tttcagaaga attttattat tatagtataa
     6901 cttttgaatt agtttccata acatttggag caaggggtta aaccacgagc ttgagcttcg
     6961 cttaaagtgg caggggtata cgtaccattt ccacatttat gtgtatggta ttttgaaccg
     7021 gtcggtgtga catagacagt ttgttcatta ttttgctcag gagctgcggc ttgtgcctgc
     7081 tgagaggcct gttcagctgc tgcttgctgt cgcgcctgtt ctgcttgttg ctgtgcgatt
     7141 gcttcttgtt gcgcttgttc tgcttgttgt cgtgcaattt cttcttgctt tttccgttct
     7201 tcttctgctt cttttgcttt gattgtggaa tcaattgcag caagacggct tgtcaactct
     7261 tgtttgtttt caggtagcgc ttggatagct gctgaagcag ttgcatagtt ttcttttgta
     7321 ggatttgctt cagcttgtga caaagcagat tccgcttctg tcactttttt ttctagtgca
     7381 gctttttccg ctttttgttt ctcttcttct tttttctttt caatttgttc ttgttctttc
     7441 gatgaatctg ccgtactttc ggagatcgtg gtactgctgc tggaagcctg agctgaactt
     7501 gtgctttgag tagtagacgt agtagcacca cacccactca acaaacttcc tgcgaccaaa
     7561 aacaatgcaa ctaatttttt cactaaattt cctccttaag taaaaaacaa ccgttttcaa
     7621 taaatgtcct tttcaattgt ttacttactt gttataataa ttgtgttatg aatagtgatt
     7681 gaggaaactt aatctaatcc cgttttgata cacgggattt tttgctatag ttctgttatt
     7741 aagtatgcaa taataaaata tgtacgttat tttattatta gattattgac atttata
//
LOCUS       DACDEG010000107         7506 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.112, whole genome shotgun sequence.
ACCESSION   DACDEG010000107 DACDEG010000000
VERSION     DACDEG010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.112"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            18..452
                     /locus_tag="IV142_002419"
     CDS             18..452
                     /locus_tag="IV142_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8486017.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(530..1063)
                     /locus_tag="IV142_002420"
     CDS             complement(530..1063)
                     /locus_tag="IV142_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP8486018.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(1157..2335)
                     /gene="mutY"
                     /locus_tag="IV142_002421"
     CDS             complement(1157..2335)
                     /gene="mutY"
                     /locus_tag="IV142_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP8486019.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2328..3125)
                     /gene="recX"
                     /locus_tag="IV142_002422"
     CDS             complement(2328..3125)
                     /gene="recX"
                     /locus_tag="IV142_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP8486020.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            3232..4614
                     /gene="rlmD"
                     /locus_tag="IV142_002423"
     CDS             3232..4614
                     /gene="rlmD"
                     /locus_tag="IV142_002423"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP8486021.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4788..5366)
                     /locus_tag="IV142_002424"
     CDS             complement(4788..5366)
                     /locus_tag="IV142_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP8486022.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5688..6158
                     /locus_tag="IV142_002425"
     CDS             5688..6158
                     /locus_tag="IV142_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP8486023.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLHNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(6266..7492)
                     /locus_tag="IV142_002426"
     CDS             complement(6266..7492)
                     /locus_tag="IV142_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP8486024.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         2184 a         1498 c         1313 g         2511 t
ORIGIN      
        1 aggaaggtgg ctagaccatg aaagttgtac aaacaaaaga tacaatgagc aatatttact
       61 tagatgctgt acggatcagg catcaagttt ttgtagtcga acagggtgtg cctttgtctc
      121 gagaaataga caaagatgaa gcccattgta tccacttcgt cctgtactca gataaaaaag
      181 aaccgcaagg aactgtacgt cttttgcctt tagaaaacgg aaaaatgaaa ttacagcgga
      241 tggcgattct ttctgaatac agacaccaag ggttaggaaa aatattgatt gaagaagcag
      301 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
      361 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      421 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      481 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      541 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      601 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      661 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      721 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      781 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      841 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      901 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      961 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
     1021 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
     1081 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
     1141 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
     1201 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
     1261 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1321 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1381 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1441 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1501 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1561 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1621 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1681 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1741 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1801 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1861 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1921 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1981 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     2041 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     2101 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     2161 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     2221 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaataaact
     2281 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2341 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2401 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2461 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2521 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2581 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2641 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2701 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2761 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2821 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2881 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2941 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     3001 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     3061 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     3121 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     3181 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     3241 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3301 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3361 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3421 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3481 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3541 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3601 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3661 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3721 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3781 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3841 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3901 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3961 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     4021 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     4081 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     4141 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     4201 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     4261 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4321 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4381 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4441 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4501 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4561 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4621 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4681 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4741 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4801 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4861 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4921 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4981 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     5041 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     5101 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     5161 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     5221 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     5281 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5341 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5401 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5461 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5521 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5581 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5641 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5701 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5761 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
     5821 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
     5881 atttgttaca taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
     5941 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
     6001 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
     6061 ggctgcatca tcgcctgaca atgtggctca tgattcctta ttatttatgg gtactattcg
     6121 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
     6181 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
     6241 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
     6301 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
     6361 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
     6421 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
     6481 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
     6541 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
     6601 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
     6661 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
     6721 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
     6781 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
     6841 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
     6901 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
     6961 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
     7021 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
     7081 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
     7141 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
     7201 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
     7261 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
     7321 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
     7381 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
     7441 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
     7501 tctgtt
//
LOCUS       DACDEG010000108         7448 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.113, whole genome shotgun sequence.
ACCESSION   DACDEG010000108 DACDEG010000000
VERSION     DACDEG010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.113"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            168..362
                     /locus_tag="IV142_002427"
     CDS             168..362
                     /locus_tag="IV142_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486025.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="IV142_002428"
     CDS             631..930
                     /locus_tag="IV142_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486026.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="IV142_002429"
     CDS             complement(1027..1461)
                     /locus_tag="IV142_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8486027.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="IV142_002430"
     CDS             complement(1693..2403)
                     /locus_tag="IV142_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP8486028.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="IV142_002431"
     CDS             complement(2393..3253)
                     /locus_tag="IV142_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP8486029.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="IV142_002432"
     CDS             complement(3258..3896)
                     /locus_tag="IV142_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP8486030.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="IV142_002433"
     CDS             complement(3911..4210)
                     /locus_tag="IV142_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8486031.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="IV142_002434"
     CDS             complement(4253..5737)
                     /locus_tag="IV142_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP8486032.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="IV142_002435"
     CDS             complement(5757..6218)
                     /locus_tag="IV142_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8486033.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IV142_002436"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IV142_002436"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP8486034.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
BASE COUNT         2261 a         1577 c         1177 g         2433 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagaca
//
LOCUS       DACDEG010000109         7289 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.114, whole genome shotgun sequence.
ACCESSION   DACDEG010000109 DACDEG010000000
VERSION     DACDEG010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.114"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            193..1824
                     /locus_tag="IV142_002437"
     CDS             193..1824
                     /locus_tag="IV142_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP8486035.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1827..2906
                     /gene="uxuA"
                     /locus_tag="IV142_002438"
     CDS             1827..2906
                     /gene="uxuA"
                     /locus_tag="IV142_002438"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8486036.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            2980..4407
                     /gene="uxaC"
                     /locus_tag="IV142_002439"
     CDS             2980..4407
                     /gene="uxaC"
                     /locus_tag="IV142_002439"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP8486037.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            4491..5138
                     /locus_tag="IV142_002440"
     CDS             4491..5138
                     /locus_tag="IV142_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP8486038.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            5267..6643
                     /locus_tag="IV142_002441"
     CDS             5267..6643
                     /locus_tag="IV142_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8486039.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            6654..>7289
                     /locus_tag="IV142_002442"
     CDS             6654..>7289
                     /locus_tag="IV142_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8486040.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAAN"
BASE COUNT         2525 a          961 c         1502 g         2301 t
ORIGIN      
        1 aagagaaatt aagctttaat ttaagttagt tctaaaaaat atatattggt gtgcttttac
       61 taaaaaaaga cacaataatt ctgacatggt agaaaaaaga atatttttag ttttttctta
      121 ttgacagcga ttacattcga ttttatacta caattgtaac ggaaactaac atatcagtat
      181 ggaggggtaa atatggtttt gattaaagac tggcaagatt ctatcgaagg attgaacaaa
      241 atgcaagttc aactaccgta ttttaatgtc gaagcggtca aacaagaagg tcagaaacat
      301 ccagtgtggc ttcattttgg tggaggaaat ctttatcgag gatttcatgc agaaattgct
      361 caatgcttag cagatcaaaa tgaattagaa tccggagtag ttgtgtgtga gacatttgat
      421 gaagaagtag tagaaaaagc ttatcatgac tacaacaatg atattttgga gattgttatg
      481 ggtgaagatg gtgaactcaa aaaaagactt ttagcagtaa cagctgatag tttatattgc
      541 aatccaaaga atataacgga ctatcaaaga gtggttaaat attttgaaga tcctacatta
      601 cagtttacaa cgtttactat tactgaaaaa ggatacagtt taaaagattc taaaggacaa
      661 tttttttcgg tagtggaagc agatataaaa tctggaccta ccaatcctca acatacgatg
      721 agtattgtga catccttgtt gtatagcagg tttaatgctg gagagttacc gattgcgatg
      781 gtttctactg ataatttctc acagaatggt caacgttttc aagatagcat ttttatgatt
      841 gcagaagctt ggcaaaaaaa aggatacgtt tctgaaaaat ttattgctta tttaaaggat
      901 aaagaaaaag tgacttttcc ttggagtatg attgatagaa ttacgccaaa tccttcggaa
      961 agtgtcaaaa tggatttatt gaaggcgggt ttcgaagact tagatataat tcatactgcg
     1021 aaaaatacca atatcgctgc ttttaccaat actgaaaaag tccattatct agtcattgaa
     1081 gatagttttc caaatggtcg acctaaactg gaagcggctg gtgtgattat gacagataga
     1141 gaaacagttg ataaagcaga tatcatgaag gttacgacat gtttaaatcc gctccatacg
     1201 gcattagcaa taacaggttg tttgttgggg tataaatcaa ttcaggcaga aatgcaagat
     1261 gatgatttgg ttggcttaat taaagggata ggttatgtag aaggcttacc agtagttgaa
     1321 agtccaggcg ttattgatcc aaaacaattt attgatgaag taatcaataa acgtttgcct
     1381 aatccctata ttccagatac accgcaacga attgcaacag atacttctca aaaagtagcg
     1441 attcgctatg gtgaaacaat taaaaagtat gtcgaaaaag acggaaatgc agaaaatcta
     1501 acatttattc ctatcgctat tgctgcttgg ttacgttatt tattggcagt cgatgatcaa
     1561 ggaaaaaatt ttgtgccaag tcctgatcct ttattggtag agttgcagga aaaattctca
     1621 gtattgaaat taggtgaaca agatcctgca aagattcatt gtgcggtaca ggaaatctta
     1681 gaaaataagg aaatatttgg tttagattta tatcaagttg agataggaaa gaaaattgaa
     1741 aaaatctttt gcgaaatgct agtaggtcct caagcagttc gtaaaacaat caataaatat
     1801 ataacagaag ttggaggaaa gtaactatgg aaatgacttt tagatggtat ggggcaaatg
     1861 aagaaaaaat ctcactacaa gaaattcgtc agatcccagc aatgaaagga attgttggaa
     1921 cattaatgga tataccagcc ggagaggtct ggccgagaga acgaattaaa gctttaaaag
     1981 atgaagtcga agccaatggc cttaccttga aagttattga gagtgttaat gttcatgatg
     2041 atatcaaaat tggtttgcca acacgagatc aatacatcga gaattataaa cagacgattc
     2101 gcaatttggc agaatttggg attgaggtta tctgttataa ttttatgcca gtctttgatt
     2161 gggtaaaatc agatttggat tatcatttag atgatgggtc ttctacgtta gcctatattt
     2221 caaaaaatat tcctactgat ccagaggaaa ttattagaaa agtagaaaat tctactaata
     2281 attttgaatt gccgggttgg gaacctcatc ggttatcaca aatcaagcaa ttattagaag
     2341 cctataaaga agttgatgaa gcgaaattgc gagagaactt tgcatatttt ctcaaagcaa
     2401 ttatcccgac atgtgaagaa gttgggatta aaatggcagt tcatccagat gatccaccat
     2461 attctatttt cggattacct cgtattgtta aaaatcgtga cgatttagaa tggttgtgta
     2521 atgtagtgga ttctccttct aatggtatta cgttatgtac tggaagtatt gcagaacgac
     2581 cagataatga tgtctatgaa attctatctg aattttgtca acgggatcgg attccttttg
     2641 cacatgttcg taatatcaaa tatttaagtg ataatggact cgatttttat gaagcgccgc
     2701 ataaatcaga atatggttct ctagatatgt ataagatatt aaaaacaatg tacgataatg
     2761 gatttaatgg ctatattcgt ccagatcatg gtcggatgat ttggggagaa accggtagac
     2821 ctggttatgg gttatatgac cgagctctag gtgcagttta tttaaatggt atttgggaag
     2881 cgcttgaaaa gggggcgagt aaataattat ataatggagt ctatttagaa gaaaaatttt
     2941 ctcttctaaa aactaaggga aggaaaggag tgcttaaata tgtttttaga taagaacttt
     3001 ttattagaga atgaaatagc taaaagactt tttcataaat ttgcagaaca ccaaccaatc
     3061 atcgattatc attgtcattt agacccagca tgtttgctag aaaataagca atacaagaat
     3121 ttgactcagg tatggttaaa tgataaagga gctggagatc attataaatg gcgattgatg
     3181 cgagctaatg ggatagaaga agagttgatc agtggtgcag gtaatgatta tgaaaaattt
     3241 ttagcttttg taaaaacagt ggagaaggcg ccaggtaatc ctatttttga atggtctcat
     3301 ttggaattac aacgttactt tgatattgat ttgacaattt ctgagaagaa tgcaccagaa
     3361 atttggcgac gtgccaacga aaaaatccaa acggattcct atcgcccaaa agaattgctg
     3421 aagatgatgg atgtgaaagc catttgtaca acggatgatc ctacagatga cttgcgatat
     3481 catactgagc tttctaaaca ggataattct ttcaaggtat tacctacttt ccgtccagat
     3541 aaggtctgga gtatcaaatc atcaggattt tcgaactata ttttaaaact tagtcaagca
     3601 gcgggtatac caatcaaaaa ctttaatggg ttgaagcagg cattgagcca acgagtggat
     3661 tattttcatc aaattggctg tcgattagca gatcacggtt tgaatactta tttttatgaa
     3721 atagcagatg aaaaaatact cgacaatctg ttaataaaag ctttagcagg aaaaacggat
     3781 ttccatttat cagaaattgc acaatttgtt acaggtattc aattacattt gatgtctgag
     3841 tatacaagat atggttggac gatgcaaatg catttaaatg ctttaagaaa taattcaagt
     3901 atattaaagg aacaaattgg agtagatgtc ggtggtgatt cgatggggga tcaagctagt
     3961 ttgacacaaa atgttgtagc attgttcgac actgctgaaa agagtgggtg cttaccaaaa
     4021 acaattttat attcattaaa tccaaccgat tggcttccac tagcaacagc aatgcaaagc
     4081 ttccaagggg gtatgattca aaaaatccaa ttgggatgtg cttggtggtt caatgataca
     4141 gctgaaggaa tgcgtcaaca gttgactacg atggcgcaac aaagtttgtt gtcgaatttt
     4201 acaggtatgt tgactgattc tcgaagtttt ctaagctatc ctcgccatga gtatttccgt
     4261 cgagtattat gtcaattact aagcgagtgg gttaaagcag gtcgaattcc agatgatgaa
     4321 atgtatctta gtcagatagt ggagaatatt tgttatcgta atgcgtataa ttattttaat
     4381 ttttttgaaa tcgaaagtag aaaataatac aaaaaataaa agcttaaatt tatttattgt
     4441 aagttagtac aactagctag agtatgtatc cataaaagaa ggagatagat atgaaaaaag
     4501 tagaaatttt aagtaagtta gaagcggctg gagtcattgc ggttgtacgt ggaaaaaata
     4561 aagatgaggc cattaaagca ataaatgcaa ttactgcggg tggaattaaa gggattgaac
     4621 taacttttac agttccgaat gctgaagaaa taatcaaaga aattgctaaa aaatatgctg
     4681 atcaaaaaga tgtaataatt ggagccggta cagttttaga tgcgtttact gctcgtattg
     4741 caattatggc tggagcagaa tatatcgtaa gtccatcttt tgatcgacaa acagcagaga
     4801 tttgtaattt atatcaaatt ccttatttac caggttgtat gacaattact gagataaagg
     4861 aggcacttac cgctggagta gaaatcatta aactatttcc aggttctgct tttggtccat
     4921 ctgttgtaaa aacgtatcaa gctccattac catatgtaaa tataatgcct actggcggag
     4981 taagtctaga taacttagaa gagtggtttg aagcaggcgt tgttgcagtt ggtgtaggtg
     5041 ggaatttgtt ggctcctgtt gaaaaaggtg attttgaaga agtaactcga atagctagta
     5101 aatatataga taagttttat gagataaaga attattagga gaaaaaataa ataatactat
     5161 aaaatgtaaa aaataaatac taactcttta tacttcagag tatagtacgt aagttggcag
     5221 ttcaacagaa tgaaaacgct taacaatagg aaggagcgca aattatatgg aaagaaaatt
     5281 tggtctaaga gacaaaatag gatatatgtt tggagatttt gggaatgatt tcactttcat
     5341 ttttgctagt tcttttttaa tgatttttta tacaaaagta ttggggataa gcggagctac
     5401 cgtaggaaca ttatttttag tagcaagatt tattgatgca gttacagaca taacaatggg
     5461 gcgtattgtg gatggatcaa aaccatctaa aactggcaaa tttaaaccgt ggattttacg
     5521 gatgagtggt ccagtcgctt tagcaagttt tttaatgtat caaacatcaa tggcatcagc
     5581 ttctatgagt attaaagtta tatatatgta tgtaacatat atactatggg gaagtatttt
     5641 ttatacatct attaatattc cttacggttc aatggcatca gctatcactt ctgttgcaga
     5701 agaaaggaca gcgttatcaa catttcgtag tataggagct accttagcag gtatgttcat
     5761 tggcgtgtta acaccaattt ttatttatac aagagatagt tctggagctc aaattgtacg
     5821 tggtggatca acttttacaa ttgtggcagg agtattttca gtgtgtgcag ttatttgtta
     5881 cgtaatatgt tataaattaa ctgtagaacg ggttaaaagt gatgaatcgc agttaaaagt
     5941 ttcattgttg aaatctttaa aaggattagc taaaaataga gcgttgcttt ctataatagc
     6001 tgcagctatt tttttgttgc ttgctcagct tttaacaatg agtatgaaca attatgtatt
     6061 tccagattat tataataatg cacagggaat agttttaatg aactttatca atcctatttt
     6121 gatgttggta cttgtttcac ctgtaagttt aattttcagt aaaaaatacg gtaaaaaaga
     6181 attggcgtca gtagggatgt tcttctctgc tattgtatat ttacttttat ttttgataaa
     6241 gcctagtaat atgtatgtat tcttagttct tacttctgtt ggatatatgg ggttaggtat
     6301 ttttaataca gtcatttggg cgaatattac tgatgtaatc gatgatcaag aagtgaaatc
     6361 tggccaaaga gaagatggaa ctatttatgc agtatattct tttgcccgaa aaattggaca
     6421 agctttagct ggcggtgtcg gaggttggac tctttctata atagggtatg attcgttagc
     6481 aaaagtacaa agtggagaag ttttacaaaa gctgtataac tcttcaacgt tgattccagc
     6541 agtttgtttc tttgttgtag gattgatttt atttttcgta tatccattat ctaaagaaaa
     6601 agtattagaa aattataatt tgctaaatca aaaacataac taggagaaaa aacatgaaca
     6661 gttggatatt ttgtattgat tcagatggtt gtgtcatgga tacaatgact tataaacaca
     6721 atctcttttt cgggccaatt gctgcagaga tttttgatgt tcatcatagg gaaaaattcc
     6781 tacaggattg gaataatatt aatttatatt cagagactcg tggaataaac agatttttag
     6841 gtttagttaa aacattagaa aaaaatgatg taccatcgat taatcgatta aagcactggg
     6901 tatctgtaac aaaagaattg tctaatccat ctttgaagag ggaaatagaa tactctcagt
     6961 cagaggattt attaaaagct ttaaaatggt cggaagaagt caatcgacag attagtcaag
     7021 ctaaaggatt agataaaccc tttttaagtg ctagagatac tataaaagct ctgaaaaaat
     7081 atgggaagat aattattgta agctcagcta ataaggaagc tattcaagaa gaatgggaaa
     7141 gacatgaatt attatcctta gttgatgaac tgtgttgtca agataaagga aaaaaagaag
     7201 acattattcg aagtgtaatt gaaaatggtt gtgatttgga taagatatta atgattggtg
     7261 attctcctgg tgatctagaa gctgcaaat
//
LOCUS       DACDEG010000110         7286 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.115, whole genome shotgun sequence.
ACCESSION   DACDEG010000110 DACDEG010000000
VERSION     DACDEG010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.115"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..610)
                     /locus_tag="IV142_002443"
     CDS             complement(<1..610)
                     /locus_tag="IV142_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8486041.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYG"
     gene            complement(623..2599)
                     /locus_tag="IV142_002444"
     CDS             complement(623..2599)
                     /locus_tag="IV142_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8486042.1"
                     /translation="MAINRYVKKAGMIVGMLGIIAGCFQGYRAYAEDVTQKTPPERVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGELVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGEEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            complement(2652..3323)
                     /locus_tag="IV142_002445"
     CDS             complement(2652..3323)
                     /locus_tag="IV142_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP8486043.1"
                     /translation="MKKWLFGFLGVALIVVCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIKMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAKMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            complement(3405..4571)
                     /locus_tag="IV142_002446"
     CDS             complement(3405..4571)
                     /locus_tag="IV142_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP8486044.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTKFTVIDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFIILEILPKKVFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFVLSIILLLSFLWLL
                     VRKRELTNGQLTFLWAFLAIMCQSFMEETLTTISWVPIVFLMLAMATHQYKTTR"
     gene            complement(4577..5266)
                     /locus_tag="IV142_002447"
     CDS             complement(4577..5266)
                     /locus_tag="IV142_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HAP8486045.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDGVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYLDTEMGNGNYILAGYNVDTPSVLLSDMTDLSEGEEIDLIDA
                     HYKYQYEVSKRGKISDYSQVVQSKENNSFLSLPKAGKKPLLTLLAFQANNMKKCDVIQ
                     CELVNVEKINK"
     gene            complement(5393..5632)
                     /locus_tag="IV142_002448"
     CDS             complement(5393..5632)
                     /locus_tag="IV142_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486046.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMKIT
                     GKEKACLLGSMKNFEQLRRTYVKTGSNCQLLLEVS"
     gene            complement(5699..5938)
                     /locus_tag="IV142_002449"
     CDS             complement(5699..5938)
                     /locus_tag="IV142_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486047.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            complement(6052..6407)
                     /locus_tag="IV142_002450"
                     /pseudo
     CDS             complement(6052..6407)
                     /locus_tag="IV142_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
     gene            complement(6394..6723)
                     /locus_tag="IV142_002451"
     CDS             complement(6394..6723)
                     /locus_tag="IV142_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486048.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
BASE COUNT         2116 a         1541 c         1067 g         2562 t
ORIGIN      
        1 tcccatacac taggaaacgg tgtgtgttga tccccttagg cgtacatgtc attagcgtta
       61 ctcgatcttc attgggattt ggttctaagt aagtgacaaa gtttctttgt agttcgtctt
      121 tttgcacttc ctcaatccga tcgatttgat aagcgtgctg ctcaccatca actttgatat
      181 aaaacttttc tcccttttta actgaaggca aatcatcgaa aagattatct ttataaagtc
      241 cactgtgccc tgcgattaaa ggatttttac cgtttcctcc tgtaatatcg ccagttcctt
      301 ccgtaatccc tacaccattc tctaaaattt tgtcggaagt tccgtcataa attggtagtt
      361 ttaaagaaat acttggaatt gtaagaatac caacaggatc gcctaactca atcttagcaa
      421 catccaaaga agaatccgtt tctgctgccg ttcctgttcg atcgattgcg gcaatctgct
      481 gattttgttg gtatttcgcc acgctcgctt cagcaggcat caaaagtaag acaccaacag
      541 ctgaaagtaa ccagaaagca atgatccatt tacccatgat tcgctttttc ttagggtgtc
      601 tgataatcat tagctgatcc ttctaggctt tcttcttaaa gtaaacaact gcagaaattg
      661 cgcctgcccc aagcaacaag caagagatca atacgtaaga ttggaaaccg ttgctaccag
      721 tcattggtac ggaagctttt tgattatttt tgatcgtgat tcggttttct tctttgtatg
      781 aatctttgtc tactttgaaa gagatctctt ttgttaattt ttgatagttt tcaggagctt
      841 ttgtttcttc cagtgagtaa tctccttcag ttaaaccagt gatttcaaaa tgaccctctt
      901 tatctgattt taggattgct gcatttgcta catcgtctac ccaaactgct tctttgacac
      961 catctttatc tgtatcagta agtccaccgt agtacttacc ttctgcattc ttagctgcaa
     1021 attctgcgcc ttcaagcggt gttgctttga tacctgttcc aaataggccg ctatcgactt
     1081 ttaagaattt tgcgccataa gtcacaacgt cagctttcgt tttttcgtgg tgttcttcct
     1141 gattaaatgg attattgtta taaacgaaat ccacgctatt tgttaatgga tttgcgattt
     1201 cagcactatc attcacaaac atgttataag tcatttttag atcttgacca tgtaaattcg
     1261 caactgtatc acttacttgt ccattattta cgataaaatc gatcacaaaa ccactatcag
     1321 aattttcgat tttatagtca gtattttctt ttaatacgac tgaaccatca ttatttgtta
     1381 ctttaatatc tgctttttca gcaacataag aaagtccttt acctgctgtg tcgccccatt
     1441 taaatacact gtaaggagaa gtttcacttt ttacaccatt tactgtaatc ccatctgttc
     1501 cacctgcgat atctgtaggg atatgaattg taccttgata attcactgcg tcaccaattt
     1561 ggaaagaatg ttcttctcct gttccatttg tcactgtttt atcgatcact ggtgctttat
     1621 agttcacgta ggaagctttt aaatcacttg tcacgccgtc attcgtaatt tcaaaggtta
     1681 acttattgtg cgcatcgttg cgagcgtctg cgcctaacaa atattggtct gctgttggtt
     1741 cttgatctga tcccactaca acttctgaag gaacttccac aaagtagtat tttcctaacg
     1801 tcaaatcagt tgcagtcagc tttccttgat catccgatac aagctctcct aattttgttc
     1861 cttttccagg ttcgcccttg tataactcaa attttgcccc ttttaaaggt gtttgtttgc
     1921 tttgagctgt gccatcgtca ccaaatttcg ttaattcaaa actcaactta gaaacaatgt
     1981 tctttggata caaatgaata tcttttaaaa atcctgatcc tgtattgtct gtcattggcg
     2041 caatcacaac cattggtttt gctttttgtg tgactaatcc agcagcggat ttactttcat
     2101 aaaccacata tacattccct ttggaattga cataggctgg ttgatcagca aaagtgatct
     2161 caccattttc atctacagct gaagtaattt tattggttgc attcttaacg tactcactgt
     2221 tagatccctt gtcttctaca tctttaacga tttcgtccac tttcgcgttg gttagatcag
     2281 aatcttcagt cggtaatact tgatccgtaa tatttcctaa cgtaaactca acatcaccat
     2341 aatctgcctt gttgtatttc gttacacctg taggatattg atcgtgcgta taaccatcat
     2401 ttttgattcc gtcaatatca acgttcaatt gcgtactttg atcgtacatt aatttatgaa
     2461 ccgtgatatt gaccctttct ggtggcgttt tttgcgtgac atcttctgca taagctcgat
     2521 acccctgaaa gcagcctgca atgattccta acattccaac aatcattcca gcttttttta
     2581 cataccgatt tattgccatt ttttactctc ctcatttcat atttcaacga ctcgcgttga
     2641 ttaaattctt gttattgtag ctctccctgt accacgtatc tttcttttcc ttgtgacgta
     2701 taaacacaag taaacaacgt taataagggt ttctctcctt tctttggtaa agacaagaaa
     2761 gagttctctt caggaacgcc atcgattaat ttaaagtact caggtacgat aaatttacgc
     2821 gttaccttgt aacgatagac gccatcgtta ctaactaagt ccatcacctc ccctaatgac
     2881 aacttttgaa tatcagaaaa taaaacacct ggcatttcca tgttatgacc tgctaggaca
     2941 tagtttcctt tgcccatttt ggcatcttca taaaaataag tagccgctcc aagactcaaa
     3001 gtgtattgat ttgccccttt aaaaattggc aatgctaaac caagtgcagg aatctgaatt
     3061 tgtgcaaata cttgtttatt gacgcccatt ttgattgctt ctttttgtat ggacaacatt
     3121 tgccccaaag acatatcatt attgacgttc gttgcatcct ccttataact ctttgcgtgt
     3181 gcattgattt gatctacggg catctttttt tctatatttt gcttaaacac ctctccttca
     3241 tgtttttggt agctaacata gccaaacaca ctgcaaacga cgattaaagc gactcctaaa
     3301 aaaccaaata accatttttt catccatctg cctcctttca gacaaaacaa aaaacacttt
     3361 ttcacaacga aaaagtgttt tctcttatta ctattctatt tcatttatct ggtggtttta
     3421 tattggtgtg tagccattgc taacattaag aatacaattg gcacccaact gattgtcgtt
     3481 aatgtttctt ccatgaacga ttgacacatg atcgctaaaa aagcccacaa gaatgttagt
     3541 tgtccatttg tcagctcacg ttttcttacg agcaaccata agaaactaag caaaagaata
     3601 atcgatagaa caaaaccaat caatccatag tgattgacga gtgcgtgata cgtattatga
     3661 ttacctagaa tctgatttcc tctatggaaa aagaaaggtt tcatccctac aaatatttgt
     3721 cctgtttttt cgcctaatgt ttcatagaat ttgcgccaaa tttcttctcg tcctgtaaat
     3781 aagtgaactt tgtctgacat tgcagcaaaa taagaaatcg gtaaagctag aaccgaaact
     3841 gctgcataca tccaaaagga caagtgtgta tttcgaaaaa ccttcttagg taaaatttct
     3901 aagataatga agataacaag cgcatataat gctgctttcg attggcaaac ccaaatcgtt
     3961 gccccagcaa gaatcaacgc tggtaatgat agcaatcgca tgtaccattt tccaaatgaa
     4021 ctagcaaatc ctgtgataag aagggctaat gtcattaaag aactacctat cgtatttgta
     4081 ttgatccaaa tatctgataa tttgttcgat ggattccaaa tactttgtcc ttctggtact
     4141 ggtatctcag taaatactcg ataaaccgtt acgagtaaaa agacatagaa tactacaaga
     4201 tacaagctac gatcaattac agtaaatttc gtccatttta caatcaaaat aatagcaata
     4261 attgtcggaa cgatcagatc accatagaaa gtatctcctc ggtagcttct aaaaaaagaa
     4321 aagaaaaggt aaaaaagaat gcctgctgca atgaagaata gatcgtttct ctttaccttg
     4381 tacagattca aaagaaacaa acagcctacg aaaaacgtca ctaactgtgc agcattatca
     4441 taaataaata atatcgtagg aggatttaac cccaccgtcc atattaaata tagaatggat
     4501 accaacagta acagaaaaat ggcactcatt ttattgatca atttttctac aagttcttga
     4561 atgtgactca cttcccttat ttattgattt tttctacatt gactaattca cactggatca
     4621 catcgcactt tttcatatta tttgcttgaa aggctaacag tgtgagtagt ggttttttac
     4681 ctgcttttgg caaggataaa aaggaattgt tttccttaga ttgaactacc tggctgtaat
     4741 cagaaatttt tcctcttttg gagacttcat actgatactt gtaatgagca tcgattagat
     4801 caatttcttc tccttcagat aaatcagtca tatcggataa taaaacgctt ggtgtatcta
     4861 cgttataacc agcaagaata taatttccat ttcccatttc agtatctaga taataagtag
     4921 aagctactcc taaactaagc gtgtcgctat tcgctccttg aaaaacaggg agaataaatc
     4981 ctgtagaaga aaaaacaagt tttccagcaa tttcaccttt gagatccttt tctaatgcct
     5041 gtttttgatt agtaatcagt tgtctcttag aaatatccgt taaactggtt aaattatctt
     5101 gataggtttt agcctgttcc tgaatatgga ttgtttttgt tacaccatct actttttcag
     5161 cgattttgtc cttttgataa ccttgataag caaaataact aaaaagaaga aaaacaatga
     5221 ttacgcccac gtaaggatta aataaagttt tattgttttt attcatctat ctaccttcta
     5281 tatatgaatt aatttaccta catctaacca agataaacca tgttaaattt tactattctt
     5341 tttattcaat aacaatgcca catcataatt gatcattttc atatcataat cgtcaagaaa
     5401 cttctaataa tagttggcaa ttggaacctg tttttacata agttcgcctc aactgttcaa
     5461 agtttttcat tgaacctaac aaacaagctt tctcttttcc tgttatcttc atatcattga
     5521 tatattgcat gacagataat cttttaaggt gtttttgttt catcattttt agcaactttt
     5581 cttccatttc tttcgacact ttttttctgt tttcgatttc ttcataattc atgagttttc
     5641 ctacctttct tgttttttgc aaaaataaaa aagcctatga tcacatacgc tctacacttc
     5701 agtcttgggt tatctttttc tttcgtttaa ttactggccg tgctggtttc aaatccttga
     5761 ttccattatg ggttctccaa taatcgaatt cttttcgtaa tctttctttt tccatatctg
     5821 ttcctttaga cagggatttg aatacatctt taggtgaata accacgagct aacagctgta
     5881 cataccttcc ttttgtcaaa gtatccatat ccatcgcctc taaaactggt cgcgccatct
     5941 aattttcctc ctaaataaat tccttactca atgcctaagg taatactacc tttttattat
     6001 tcgtcgtgac tccttctaag cctttatctg taaaatttat tcctccagtt tttattcttc
     6061 tttatccgct acaaattcaa tgatttcaac gctgacaata taatcactca aatatgacca
     6121 agtatcttca acagataatt cgtctccatt atcatccgta agcgtcattt taaaccattc
     6181 attaccttca tcatcttcag gtagcaagcc gaatccaatt ggattatcag tcaatttttg
     6241 atcgtataaa tcatcctcgt ctaatgctaa aacatctaat cctttacaat ttccgcctct
     6301 tctaaacgtc acaaatcctt tgtaacaaat cctttgtaat cccaacgttg gaaagaaacc
     6361 ttaatcgtat gtgtgcccaa tcgtatattc ggatcataat ttttcatttg ttgtaacctc
     6421 ctcataatca gttagtacta taaaggtatc tttgttttta tccaaaaaat ctaataaatc
     6481 ttttgtagaa gatatctctt catcaacttc accataataa ataccttcga taattgttgc
     6541 tatatctgaa aaactaattt ttactggaca tttatcgtct accgcattca ataccgacaa
     6601 aattgcttct ttttctgcaa tctctcgttt gaatttcttt ggagctaatc gcatgacact
     6661 caagtcattt tcaagctttt caatccatgc agttttactc attttcgcaa atttttgaat
     6721 cattcctcta ccacctttgc tatctactaa attagtttta cttcataacg aatatcttta
     6781 tcgtttttcg ttcactcaaa aatatataaa ataaatcgac tcgtttatgg cgtccgaaac
     6841 gagaaaccct agtagacagc ggtaaatgct cgattgatcc aatgatcaac gtcctcgcaa
     6901 gtatctacat gtactgcagc acacgacaca atttcttgtg cgtcctcccc attacgagaa
     6961 gacctactcc ttattccatg agtttgcgtc aaagcctaga tcccttagta gctgctcatg
     7021 attaatctag gcgctattct aacttgtcta acttctaaat tccctcttgt ttactctgtg
     7081 catattattt acacaaattc atttcaccac tatccaaaca agggcattta ctcgattgac
     7141 caatttttta cgttaccaat cgtaaaaacc taccccgtca ctccccgaag ggaattgttg
     7201 atagatcaag tctactcctt atttttctag tgattctaag ttcctttaga agtctcgaca
     7261 atattttcaa gcaagcgaaa aacgat
//
LOCUS       DACDEG010000111         7275 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.116, whole genome shotgun sequence.
ACCESSION   DACDEG010000111 DACDEG010000000
VERSION     DACDEG010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.116"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(34..1380)
                     /gene="gor"
                     /locus_tag="IV142_002452"
     CDS             complement(34..1380)
                     /gene="gor"
                     /locus_tag="IV142_002452"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP8486049.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(1514..2755)
                     /locus_tag="IV142_002453"
     CDS             complement(1514..2755)
                     /locus_tag="IV142_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP8486050.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(2773..3696)
                     /locus_tag="IV142_002454"
     CDS             complement(2773..3696)
                     /locus_tag="IV142_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP8486051.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(3917..6409)
                     /locus_tag="IV142_002455"
     CDS             complement(3917..6409)
                     /locus_tag="IV142_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP8486052.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6416..6883)
                     /locus_tag="IV142_002456"
     CDS             complement(6416..6883)
                     /locus_tag="IV142_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP8486053.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(7019..7092)
                     /locus_tag="IV142_002457"
     tRNA            complement(7019..7092)
                     /locus_tag="IV142_002457"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7056..7058),aa:Arg,seq:acg)
     gene            complement(7183..7256)
                     /locus_tag="IV142_002458"
     tRNA            complement(7183..7256)
                     /locus_tag="IV142_002458"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7220..7222),aa:Pro,seq:tgg)
BASE COUNT         2020 a         1601 c         1179 g         2475 t
ORIGIN      
        1 caataaaaag ctgttggaac tgttaatatt aaattatcgc atcgttacga attcttccgc
       61 tcctgttgga tggatggcta ctgtattgtc aaagtcttct ttagttgcac ccatcttgat
      121 agccacggca aaaccttgca gcatctcatc gacaccaaca ccaatagcat gtaagccgac
      181 gattctttct tcttttccga cacagatcaa tttcatttcg catttttggc gataatcatt
      241 taatgcaaag tacattgggg taaagcgtga acgataaact ttcaattcat tttcgccata
      301 tttttccaat gcttcttctt ctgtcaaacc aattgttgca attggtggat gcgtaaagac
      361 gactgtcgga acaagtttat agtcaagata agaatctttc tttccattaa ataaacgttc
      421 tgataaacgt ctaccagctg cgattgcaac aggagtcaga tctaatttac cgatcacatc
      481 accaacagca aaaatatgtt catccgttgt attttggtat ttatctactt tgataaaccc
      541 ttttttgtcc aattccacat ttgtattttc cagacctaat ttttcggtat tcggtacacg
      601 tccaccggca aaaagtacac aatcaccagt taaggtttca ccattttcaa atagcaccgt
      661 atattcgtca ttgtttttag taatttcttt agcaatatga tgtgcgtaag tttttattcc
      721 agcttcttgg tacatttgga tcaaattgtc cgaaagcatt ttatcaaacg ttctaagtgg
      781 tcgttctttt cgatatgccc acatgacttc actacctaaa ccacgaaata cacctgaaag
      841 ttcagccgca atgtatccag cacctaaaac gattgctctc ttaggtaact ctgttaattc
      901 aaaaaatcca tttgaatcaa ttgcgtattc tccaccagga atggtcattt tacttgcacg
      961 gcctccagta gcgatcaaga ttttaggagc acgataggtt tcaccgttta cttcgatcgt
     1021 ttgactatcg ataaaagtag cgtaaccttt tatagcctcg acatgattgc tatctaatcc
     1081 tctttgataa gcaccgtgta gaaaatcaat atatttttct cgtctttcta ctagaccggc
     1141 gaaatcaaca ttttttaaat ctgcttggat accgtaactt gaggcatctc gttcaattgt
     1201 ttccaaaatc gtacttgctt gccacataac ttttttaggt acacaaccga cgttgacaca
     1261 tgttccgccc aattcattcg cttcaatcag caaaactttt gcgccatgca tccctgctct
     1321 gtttgctgaa gcaataccgc cagagccgcc accaattacg atgtaatcat atatttgcat
     1381 tttgttcctc cttattgaag agatcaatca aaacctttat ttaacgatcc tttttctaca
     1441 gaaaacaagt gtaacacttt tcttgaattt tgtatgcgaa gatgcttaaa acagggatat
     1501 atcagctttt tccttattta cgttttcgga tcatatctcc aggtttcacc ggctgtgcga
     1561 ctggcatagt taaaatcatc attggatttg gtgctcggtc aatggattcg ccttcttcat
     1621 tatgcataac ttcaacggtt tgactgaaat gactgaatcc tggaccataa aattcgattt
     1681 catctcctac agaaaacaag tttctctgac gtatcgtagc aatctgtgtt gcctcatcat
     1741 aagataatac ttcaccgaca aatttatata ctgggatttt acgacgttca ccgaataatt
     1801 gttcgttttc agttggtgta ttgtagtaaa aacctgtagc taattctcgt tgtgctactt
     1861 tccataactc gtcgatccat tcttgcttgc agacatagtt ttcaggatct tccatataac
     1921 tgtctacagc cgctttatag acatttgaca ccgtagatac ataatggatc gatttcattc
     1981 ttccttcgat cttgaagctg tctacgccgt tttcaatcaa ttctggaata tgttcgatca
     2041 ttgacatatc cactgcactc atcgagaatt cttcttccac ttcgcctgta tcagttaaac
     2101 tgtttcgttc ttgaccaaaa ggcatatcga acaatccata cttccagcga caagattgag
     2161 aacatccgcc acggtttgca tcccgcatcg acatatggtt ggacaatgtg catcgaccag
     2221 aataagagat acacatggca ccatggataa atgcttcgat ttcggtatcc gtatttttac
     2281 gaatctctgc cacttcctcc attgaaactt cacgagctaa aacgacacgt tccaaccctt
     2341 cttctttcca gaattccaat gtctcaaaat tagttgctga agcttgagtg gataaatgga
     2401 tcggcagacc tggggcttct gtcgcacaaa tttcgatcaa agctggatca gaaacaatca
     2461 ctgcagaaat accgacatca cgcaaactgc ggaaaaattc ccctgctcca tcttcatttc
     2521 cttcatgggt gaccatatta gcagcaacgt ataccttcgc atcatgttct tttgcaaaag
     2581 caactccttc agccatttct tcataagtaa aattacctgc acggcttctt aaaccatatg
     2641 cattcccgcc aatatatact gcatctgctc cataatggat cgcggttttt aacttttcca
     2701 gtgttcctgc tggcgctagg atttctggtc gtttcaattt tctttttact gtcatttctt
     2761 tcatcatact ccttatttga tagcagaagg atcaatataa aagaatcctg tatctaattc
     2821 acggttttca ggatgcaatt tactgatttc gctagtcatt ttttccgctt tttcttgatc
     2881 ccagctacct tctaaaattt gtttttttgc ttcggcgaat agtcgagtga tgtttacgaa
     2941 tttttcgcct ggagtaaata atccgtccaa tttccatgta cagtaatgat gctgataaag
     3001 ttcattcaat tcattcgaga gattcagatc attgctggca aaaatatgtg tgccatgact
     3061 atcttcataa atagaataat gtgtctcttc ttttttaggt tccgagataa aaagacctct
     3121 ttcacgatct ttttgttcgt cttgtttcgt atagttatag taattctgta gcagtggacg
     3181 tttggattga tggatgcatg tagcaccata cacaagggtt tctacaggaa tatcgagttt
     3241 ttcttccatc gccttcatct cttcaaaagg aacttcacga gcaagtactg ctccaactgc
     3301 gccttttttc gcccagaaat tgatttgacg tgcgctcgtt actaatgttt cagcatcata
     3361 aatatacggt atacgcaatt caggattttt tttcatcgta taaataatac ctgtatcacc
     3421 aactgtgatc acatctacgt ttatttcttt taaaaaagcc aaatattcag gtacattttt
     3481 cattttttca ggatgaatga tcccatttac agcaatattc gcttttttac catacttgtg
     3541 tgctaattct accaattgtc gctgttcttc tcgtgtaaat gaagcaggca gacgcaatcc
     3601 aaattgctct tcaccgaaat atagtgtatc tgcacctgca gcaagcaact gttctgcttg
     3661 ttcaatagtt tctactgtgg ttatgagttc gatcataatt ttctcctcct acaacttcat
     3721 aatagtagca tagctctaaa aactttccaa tacttcttct atttaaagtc ggatttttga
     3781 aaattatttc acaaaaaatc aaaataaagt tcgatatatt ctgtccaaat ataaaaagag
     3841 attgtgacaa aaatcttgtc acaacctctt aatcaagtct tactattata aaaattcagc
     3901 tacacaggcc aagtaactat agggaaccta ctgtttcctt tttaggtccg cgaacattca
     3961 atgtgatctt accttttgaa gcacctactg ttacgtggtc acctaaacga atttctccgc
     4021 caagcaaagc ctcgctcaat cgatcttcaa tttctttttg aagtgcgcga cggattggac
     4081 gtgcaccata ttcaggatca aatccagctt tcccgatcac atcgattgca gatggtgtga
     4141 ttttcacttg aatatcttgt tctttcaatc gtttgataat cgctttgctc atgatttgga
     4201 cgattgtatg gatttcatct ttactcaatg aatggaagac gacagtctca tcgattcgat
     4261 tcaagaattc aggtcggaaa gctttcttta actcttctaa gatacgtttt tgcatggctt
     4321 tgtgatcttt agtaacatct tggacattga atccgacatt tttttcttct cgaatttgtg
     4381 tcgctccgat atttgaagtc ataatcatga ttgtatttct aaagtccact tttctacctt
     4441 tagaatctgt taaatgacca tcatccaata cttgcagaag taaattgaaa acgtctggat
     4501 gtgctttctc cacttcatct aagagaatca ctgaatatgg tttttgacga attttttctg
     4561 tcaactgtcc accttcatca taccctacgt atcctggagg agacccgatt aatcggcttg
     4621 tactgtattt ttccataaat tctgacatat caacacggat cagggcatct tcacttccaa
     4681 acatgacttc tgaaagtgct ttggccaatt ctgtttttcc gacaccagtt ggtccaagga
     4741 acatgaatga accaattgga cgatcaggat ctttcaatcc gcttcgtgca cggcggatcg
     4801 cacgtgaaac tgctttgaca gcctcatctt gaccaacgac acgttcatgc agaagtcctt
     4861 ccagttctaa caagcgttcg ctttcttttt tctccaactg ctgtagaggg acaccagtcc
     4921 actccgatac aactgtagca acatcttctt ctgttacacg atcagaataa ccttgctcct
     4981 gttttgcttc aattgcagaa actttttgca gctttttacg tagtgctttt tcttgtctgc
     5041 gtaattgagc agcattttca aagtcttgtt tttggatcgc tgcttccttt tcttgtacta
     5101 gttcatcgat ttctagtttg atcacagttg attttgttaa gtgatcggtt tgatccaaac
     5161 ggactttcgc tgctgattca tcgatcaaat caattgcttt atcgggtagt tgacgagaag
     5221 tgatataacg aacagaaaga ttgactgctg ctcgtacagc ttcatctgtg atctccactc
     5281 catggtgctc ttcatatctt gtacgtaatc cttgaagaat cacttctgct tcttcaggag
     5341 ttggctcatc tacttgaatg cgagcaaaac gtcgctctaa tgcagaatct ttttcgatat
     5401 atttttgata ttcatctagg gtcgttgctc cgatcgtttg taattctcct cgagctaacg
     5461 ccggtttcaa gatattcgaa gcatcaattg ctccttctgc tccacctgca ccaattaagg
     5521 tatgcaattc atcaatgaat aagattactt gaccatcatt atagatttcg tcgatgacct
     5581 ttttcattcg atcttcaaat tcaccgcgat atttcgtacc agcaaccaaa gatcccatat
     5641 caagcatcat caggcgcttt tcttgcatgt cttctggcac ttcacctaaa atgatttttt
     5701 gagctaaccc ttcagcaatg gctgttttgc ctacaccagg ttcacccacc agtacagggt
     5761 tattctttgt ccgtcggcta agaatctgga taagccgttt cacttctctc gaacgaccaa
     5821 caactgggtc cagcttgttt tcttttgcta atttcgtcaa atcacgtgct aatgaatcta
     5881 gcgtaggtgt tccttgcggt gtttgccgtt tatcttgtac atttacattt ctacgacgat
     5941 tcattccttt tccagcagaa tcaccagcta gccccatttt ttgtttcaga acttgacggg
     6001 cttttgaaag gctgatacct aagttgatca tgattcgagt agccatgata ctttcatctt
     6061 ttaataagct catcaaaata tgttctgttc caactttttg agaaccagtt ttcttcactt
     6121 cgctgctagc taaagcaaaa acttgtttca ttcttggtga atatggaaga aagactcctt
     6181 tagggtaaga acggatacca ccataaccaa tcagatgttc aatttcttca taaatttccg
     6241 tttcattcaa atccatttca cgtaatactt ttccagcaat tccattttgc tcgaccacta
     6301 aggctagtaa aacatgttct gaactaactg attgatgttt caaccgtttt gcttcttctt
     6361 gtgcaatttc taatactgct tgggctcttt cagtaaaaag ttcttccatc taatctcact
     6421 tcccatcttc ataacttaaa cgttccagaa atgctcggag aatccgagca cgtgtatgat
     6481 cttcatattc attgaaattc aatgtgtttt ttgcaattgc actcaacatc aagtttcctt
     6541 ctttttttgt aattatttgg tcttcataaa gtttttgaat aatagcaaaa gcatttttct
     6601 cactgattgt atcatcaaat aactgattta tctgctcaag taattgtttt ttatctgaaa
     6661 tccttacttt tgcaattcgt atataaccac caccaccacg tttgctttct actagataac
     6721 cacgttggat ggtaaaacgg gtattaatca catagttgat ttgtgaagga acacagttaa
     6781 agaggtctgc catttcaatc cttcgaatct cgatcatttc actattttcc agaattttct
     6841 ttatatatga ttcaattaaa tccgaggtat tctgatgtgc catcttattc attccctttg
     6901 actaatattg accttaatta taacgaattt gataatagaa agaaagaaga acgctttttg
     6961 ttaaataaat cctttagaat caacaaaaaa aaatacggaa tatactattc cgtatcgacg
     7021 cgcccaagag gagtcgaacc cctaaccttt tgatccgtag tcaaacactc tatccagttg
     7081 agctatgggc gcatattcaa tttaaaaaaa ttataaatca tttgaaaaca aaaagtaaaa
     7141 aaattataaa tcatttgaaa acaaaaagca agataaaaaa aatcgggaag acaggattcg
     7201 aacctgcgac cccttggtcc caaaccaagt gctctaccaa gctgagctac ttcccgttta
     7261 aaaaataaaa aagac
//
LOCUS       DACDEG010000112         7210 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.117, whole genome shotgun sequence.
ACCESSION   DACDEG010000112 DACDEG010000000
VERSION     DACDEG010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.117"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..901
                     /locus_tag="IV142_002459"
                     /pseudo
     CDS             1..901
                     /locus_tag="IV142_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            919..1488
                     /locus_tag="IV142_002460"
     CDS             919..1488
                     /locus_tag="IV142_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP8486054.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            1598..3352
                     /locus_tag="IV142_002461"
     CDS             1598..3352
                     /locus_tag="IV142_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8486055.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            3306..5078
                     /locus_tag="IV142_002462"
     CDS             3306..5078
                     /locus_tag="IV142_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8486056.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            5601..5870
                     /gene="rpsN"
                     /locus_tag="IV142_002463"
     CDS             5601..5870
                     /gene="rpsN"
                     /locus_tag="IV142_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP8486057.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            5885..6064
                     /gene="rpmF"
                     /locus_tag="IV142_002464"
     CDS             5885..6064
                     /gene="rpmF"
                     /locus_tag="IV142_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP8486058.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            6096..6473
                     /locus_tag="IV142_002465"
     CDS             6096..6473
                     /locus_tag="IV142_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486059.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            6494..6643
                     /gene="rpmG"
                     /locus_tag="IV142_002466"
     CDS             6494..6643
                     /gene="rpmG"
                     /locus_tag="IV142_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8486060.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            6667..>7210
                     /locus_tag="IV142_002467"
     CDS             6667..>7210
                     /locus_tag="IV142_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP8486061.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPL"
BASE COUNT         2535 a          989 c         1369 g         2317 t
ORIGIN      
        1 ggacttcaaa gcgactaaga aacttgctga cttactttgt gatttaaaaa ataaaggatt
       61 tactattgtt atagctgaac atcgttttta ctatttgaaa gaattaattg atcaagttct
      121 atttgttaaa aatagaactg taaaaactac aactattcag ttgttaaaaa ctgaaaacaa
      181 taacgcatta aggaaatttg acatttttaa tagttcgatt gaagttccct atatcgaaaa
      241 ggtaagtgca gatgtattaa aagttgaaga aattacttat gaaaatattc taaaagacat
      301 aaattttgat gtcaaagttg gagatgttat tggtttggta gggaaaaacg gtgtggggaa
      361 aacaacctta ctaagaatac tcatgaagat tattcgccct accaaaggaa agataatcga
      421 aaataaaaaa aatttgtcat cgccgttttt agtaatgcaa gaaatggatt accaattttt
      481 cacagaatca gtgaggagcg agttagccct ggggaatgaa atagttagta aagatcagca
      541 agaaaaaatt taaaaaaaaa tggaacttta taaaatgaaa gatcaattac cgtttgattt
      601 atccggtgga gaaaaacaaa gattattggt ttctatagct tcattgtcaa aaactaattt
      661 atttctattt gatgaaccta caagtgggtt ggattatatt aatatggaaa ggattgctga
      721 ccttattaaa gacttaaaag aaaagggtgc agtagttata gctactcatg atccggaatt
      781 tctttataaa acatgcaata gaattattta tttaaaaaat ggtggaataa aaaaagatat
      841 aaaacttaga gtagaagatt caactttgat agaacagatt tttaatgaca taacgaaata
      901 gaattgagga gaatatatat gaaaacaaaa gattatattt ttattggttt agctacagtt
      961 atttccctag taatttactt tttttcaata atggttagta gtattggtgg ggcgtttggt
     1021 cacagcatat cccccgggat ttttggatta ttatctggaa tcatatttgt ttatattagc
     1081 tataactacc ctagaaaagg aatttttact atttacacaa ttgttttatt gttatttttt
     1141 acgttaatgg gcggggcgta cttaccttgg tttatcagtt cgattagtat ggcgatctta
     1201 gctgatatta ttctatctgt atttggatat gatcgagcaa ttcctcaagt tgcgtcttgg
     1261 gctctaatgc aattaggcag tgctgccgga cagtggattc ctatatggtt ctttacagat
     1321 cgttttcgtc aagattggat agataaagga caatctgctg caacaatgga tgcaatgatc
     1381 cactatgcgg taggtatatg gggaatcatt tcagtgttag ttgtagcaag tttatctatg
     1441 attggtgtat tgattggaag aaaagtctta aaaaaataca aaaaatagaa cgtagtttac
     1501 cttttatctg tttgtacatc tctaatttac gctattacgg aaagcattaa cagttgtaaa
     1561 cgaataagag aagaaattga atgcggggga ggaaaaaatg aaagtataca aaaaattatt
     1621 tagttatgtc cctaaaatga aatactacgg atggttagcc actttatttt ctgatttttc
     1681 tgtcgtatta acagtctatg gatattacag tatttacaaa tttcttcagt ctctaattat
     1741 tgtaggagat gaatatttag ctaagagtta tgcggttcaa actgttgctt ggttaacttt
     1801 aggagcttta ttttatattt tttctggctt attttctcat atccttgggt ttcgcttaga
     1861 gactaatttg agaaaaaagg gaattagcgg tttaacagaa gcaagttttc gtttttttga
     1921 tttaaattct tcgggatata ttcgaaagac aattgatgac aacgcggcta aaacacatat
     1981 ggcaattgct cacttgattc cagatagtgc tcaagccata gtaacacctg ttttatcagt
     2041 tgccttaggc tttttcatta actttaaaat tggattggtt ctaatcgcca tgttagtaat
     2101 tggtgtattc ttgttaaaga aaatgacaga gaatacaaat tttattcaaa aatatcaaga
     2161 atcgttagat gtgttaagct ctgaaacggt agaatatatc cgcggaattc aagttataaa
     2221 gatatttggt gctaaagtaa catcatttaa agccttgaat aaagcaatta acgattatgc
     2281 taagtttgca tataagtatt ctttgagtgg gaaaacacct tttgttcttt ttcaatggct
     2341 attttttggc atagtttgca ttttgatagt tcctataacc ttttttataa cttctcttac
     2401 cactcccaag atattagctg ttgacttgtt gatgttgttt tttttaagtg gaattatttt
     2461 tgtttctttc atgagaataa tgtatgtaag tatgcacttg tttgaagcaa gatatgcagt
     2521 cgaaacacta gactcccttt atttagaaat gaagcaagac caactaaaat atgggaacga
     2581 gacacagatt aaaaattgtg acattgcatt tgaaaatgtc agcttttcat acaaagaaga
     2641 gtgtgtttta gaaaatttca acttagtttt agaacaaaaa agaatttatg cattagtagg
     2701 aaaatcagga agcggaaaga gtacgatagc taaactaatt tcaggtttct acaatgtgga
     2761 taagggacag attaaaattg gtggtaagcc tttaaatcac tattcaaaag acacaattat
     2821 taacacaatt gcttttgtct ttcaagatac gaaactattc aatcagagta tttacgataa
     2881 cgtttctata gcaaatcctc aagcgccagc aagtaaagta tttgacgcgc taagattagc
     2941 cggttgcgaa tcgattttag aaaaatttcc cgaaagagag cataccttaa ttggtagtaa
     3001 aggagtttat ctgtcaggag gagaaaaaca acgtatagct attgccagag caattttaaa
     3061 agatgcaaag attgttatta tggatgaagc cagtgcatct attgatccag ataatgagta
     3121 tgaactacaa aaggcttttc aacatctgat gaaagaaaaa acagttatta tgatagctca
     3181 tcgattatcc agcattaaat ctgtagatga aattcttgtc ttgtcagaag ggaaaatagt
     3241 agaacgtgga aatcataatg aattaatggc taaacatcaa gtgtataaac aattggtcac
     3301 taattatgag aatgcaaatg attggaggct tgacaatgaa aagtttttat aaaaacaaat
     3361 ttgctttaag tgatcagggc gcagaagctt taactaaagc ttctatttct agtttttttg
     3421 tatactgcat taatatggta ccagcattca ttataatgat gctgatagac gaattagttt
     3481 tagaaaatac aaagccccgt tggctttatt ttgccgtatc gtttgttact ttgcttttca
     3541 tgtattggtt gttagatagg gagtatgaaa acttatataa tagtacttat aaagaaagtg
     3601 cgcatttaag agtgcaaatt gcagacgatt tgtcaaattt accattatcc tatttttcaa
     3661 aacataattt atcagattta tctcaaacta tcatgtctga cgttgaaggt attgagcatg
     3721 cgatgagtca tgcaatacct aaagccggcg gtatggctct gtttttccct tttatttcag
     3781 tgatgctttt ggttggtaat gtcaaaatgg gattagctgt tattttgcca acgttattta
     3841 gttttgtctt aatcttgtta tcaaagaaat cccaaacgaa agccaatact aaatattacg
     3901 atactttgag agaaaactcg gaagaatttc aagaaactat agaattgcag caagagatta
     3961 atagctttaa tctatctaaa aaagttcaag acagactttt caaaaaaatg gaagagagtg
     4021 aaaggattca tttaaaggta gaattaagta ctttttcagt catggcctta tcctctattt
     4081 tctcatatgt tagtttagca gtagtcattc tagtaggtgt tcacttactg ttaacgggtg
     4141 aagtgactat actctacgtc gttggttact tactagccgc aataaaaata aaggattcct
     4201 tcgattctat gaaagaagca gtgcttgaaa tattttattt agctcctaaa atacagcgaa
     4261 ttagagctat gaaggaaacc agtatccaag agggatcaga tagtcctctg aaatcttttg
     4321 acattgagtt gcgagatgtc tctttctcat atgataacaa taccccaatt ttagatcata
     4381 tctcttttac ggctaaacaa ggagaagtga ctgcgttagt aggtgctagt ggttccggga
     4441 aaactagtat cttgaaatta gtgtctagac tgtatgatta tgatgaaggc tgtattttaa
     4501 ttgatgggta tgatattaaa cgtgtatctc cagcttcttt gtttagcaaa atcgctattg
     4561 tttttcaaga ggtaactttg tttaatacaa gtattttgga aaatatacgt attggaaata
     4621 gtcaagcgtc agatgaggaa gtgaaaaaag cagctcgttt agctaactgc gaagacttta
     4681 ttgaaaaact acctgacggt tatcatacat tggtcggaga aaacggaagt tcactttctg
     4741 gaggagagcg acaacgccta tccatagcac gggctttttt gaaaaatgcg ccaattttaa
     4801 ttttagatga aattactgcc agtttagatg cggaaaatga aaaacgtatt caggaaagtt
     4861 taaatcgttt aatacaagat aagacagtac taattatatc gcatcgcttg aagtctattg
     4921 aaaaggtaaa taaaattgta gtgatggatc aaggaaaact agttgatcaa ggtactcata
     4981 atgagttgta tcgaaggtca gaaatataca aaaatctcat aaagaaaact aaactatctg
     5041 agaaattcgt ctatgaaaaa gaagctcaat cacgatgatg aaataggctt taaattaacc
     5101 tacaattttt gggatatagt tataattctt tttcaaaaag aataatttta ttaaacagca
     5161 tctaattttg aatacacgaa agaatcagta tgattgaatt agttcactta atgtctattc
     5221 ttataatata aggaataatc aaagttttaa aaaattggcg ataaaccagt taaacataaa
     5281 agattgaata aaaagttata gtttctttca tattgccgtt ttaagtagag gaaaagctac
     5341 ttaaaacggt atttgtgttt gattggacta ataaaagtga attcttattt ttcagagcct
     5401 aaactgcata ttcttttaaa tgatttgatt atctctatgt accgataaat tcgttttcaa
     5461 atagttatct attcatatga gttttatttc atgttattta ttgttgacaa gagcaactct
     5521 tgttggtaaa gtatcagtgt caaaacgtaa cgattacgat ttgaggtgtc taaatcaaaa
     5581 taaaaggagt ataaaggtat atggcaaaaa agtcgaaaat tgctaaagca aagaaacaaa
     5641 tggcgatgat tgaaaagtat gcagataaac gtcaggaatt aaaagccgca ggagatcgca
     5701 ccgctctcgc taaattgcca agagattcaa atccgaatcg tctgaggctt cgtgatcaga
     5761 cggatggtcg tccaagaggg tatatgcgaa agttcggtat gtcccgtatt aagtttagag
     5821 aacttgccca tcaagggttg attcctggag taaaaaaagc aagttggtaa aaggaggaaa
     5881 tatcatggca gtaccagcaa gaaaaacatc taaggcgaag aaacggttaa gaagaactca
     5941 tcaaactgta gtaaaaccag aaatttcatt tgacgaagcg aatggtgatt atagacgtag
     6001 ccatcatgta tctttaaaag ggtactataa tggaaagaaa gtaattaaag gagcttcaaa
     6061 atagtttaat atataacttt taggagggaa tcaaaatgga aattatttac ccacctttag
     6121 ttgaagaagg attaaagtat tatcttgaaa cgacccagca atcgctagat aaatgcactt
     6181 tttatcgttc gatggtagag agaggaatta ttactgaaac gggtttaccg acccaacagg
     6241 caattgaaaa tggattagtc aaagattact atgaggacca aggcttatca tttgatgatt
     6301 ttttaagaat ctatccaatt tttgaagagt atgacgagga gttatttcaa tgtatcgatg
     6361 gttattggga gatacctgtt gatatgaaag aaaatttagt ttcacaattg gaatctggcg
     6421 agttaacttt tgaagatgca caacaaattc aagcatatct tgaagatcga taagtattga
     6481 aggaggaaga tttatgcgtc aaaatattat tttggaatgt gtcgaaactg gagaacgtct
     6541 gtatctcacc agcaaaaata aacggaataa ccctgaacgg ttagaattaa aaaagtattc
     6601 gccaaaattg cgcagacggg caatttttaa agaagtgaaa tagctaagtg gtaaaaaggt
     6661 gacaaaatgg gcataccagt tacaatcgtt tctgggtttt taggggctgg aaaaacaacg
     6721 ctaatcaatc aagccttgca agtctcaccc taccccaggg aagaaattat tataattgaa
     6781 aatgagtttg gtgaagttgg gattgatcac aaattattag ttcatagcga ggaacgggtg
     6841 ttacaactaa ataatggatg tatgtgttgc agtcttcgca gtgatttatt agcaatattg
     6901 atttctttac tggaaacaat agaagaacat caacaaccaa tttcacaaat catcattgaa
     6961 actacaggta tagcagatcc ccaaccaatc attcaaacac ttttaacagc gcctcaacta
     7021 agaggaagag tatatatcga tagtttattg actgttattg atagtgacag ttttgaactt
     7081 attgaaagag aaccacaagc ccttaagcaa cttgtcatgg cagatcgcat ttttgtgtct
     7141 agaaaacaaa acttgaattt atctgcacta aaaaaattac agagagaaat caaaagtatt
     7201 aaccctttat
//
LOCUS       DACDEG010000113         6633 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.118, whole genome shotgun sequence.
ACCESSION   DACDEG010000113 DACDEG010000000
VERSION     DACDEG010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.118"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(718..3867)
                     /locus_tag="IV142_002468"
     CDS             complement(718..3867)
                     /locus_tag="IV142_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP8486062.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3880..5079)
                     /locus_tag="IV142_002469"
     CDS             complement(3880..5079)
                     /locus_tag="IV142_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP8486063.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(5069..6607)
                     /locus_tag="IV142_002470"
     CDS             complement(5069..6607)
                     /locus_tag="IV142_002470"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP8486064.1"
                     /translation="MDASEYKNYLLGLIFYKYLSDRLLEQVVLLADESLEEYDTVSKQ
                     TMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLADQAKQATFQLNDLNKAFVQ
                     LASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVDVLEHDGDVIGDAYE
                     FLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVR
                     NYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTF
                     DAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIV
                     LPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVL
                     FIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPR
                     YVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAISSLQTTPENEMWLQGALEVF
                     KHEQ"
BASE COUNT         1844 a         1369 c         1089 g         2331 t
ORIGIN      
        1 ggactaatac aggtagtcct cttttttgtt attttgatat tctaaatcat tttttattgg
       61 attattccct tgttcgatat agacccaaat tgatctatca ataattatca aactgagtca
      121 tatagagaca aaaatttcaa aaatgacctg ttaaatggtc atcgaatatc atttaaaatc
      181 aatacgacgg agtcagacac ggagtcaaaa caagcccgct gagagtaaaa aatatataca
      241 gtcccatagg taaagtgcca caggactgta tattttttta tttgagatta cctgaaatga
      301 ttccatttac tttttatctc attttttcct actagttagt atcaaatact ctcctatttt
      361 ctcaataaac tattttgggt tacatggtat ctgaaacagc taaaaccttg tcattacagc
      421 aaaaaatcac tttatctccg tttcatttca gatacatttc acttaagcgg attccaaaaa
      481 aacaaaagaa aaaggaaccc ctagttagcc tatgcttttt attggttcct ctcaaaacac
      541 ggtcatataa caacggggtg gggtaaatga atgaaaaagg tggaggaaac attattcaat
      601 agctgtctta aatacttcgt tataaaatgt gaacaaataa aagacatcta tccctatttt
      661 ctacacttcc actttactac catctttgaa tcgtacaatt ttcttaactc ttctcgttta
      721 cctcaattca ttttcaagtg gaatcagctc atcatctaac attttacgcc atgctcgctt
      781 catttcttgt gggtatttaa atggcttcgc ttctggatgg ttggctttat atccttcgta
      841 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
      901 cagattagaa gcatgtaatt ctttctcaga aacaaaccac tcattggaaa agacatgaat
      961 caaatcatca ctcgcttgtg taaaatagcg acgtttaatg acaaagatat cttcattact
     1021 atctaaagtc cccacatcaa acgcatttaa aatttgtcgg taaacttctt tgactccttc
     1081 tgttttattc aatttagcca aagctttttc aatttcttct cgtgcatctg aactattcgc
     1141 cgcataagtt cctagtaatt gatcaatata agtagaatca atatcataca acttcgtggt
     1201 attttcacta taaaattcga tcgtggaaaa atcttctccc tctttttctg gttcttcctc
     1261 tattaatgaa cccttcacgg tctcgtacac gccaatttgt tcttccaaaa gttgaatttg
     1321 attgttcaag gttgaagatt tcaccatgtc atcattgtaa tcgtcataag tcactaacga
     1381 ttcataagca cgattcaatt cttggaatgc tttgacgtac tcgatacgga tttcaagtgg
     1441 cgtatgttca tcaatggatt ccatggatga aacctctttt aacttaccat atgcttgctt
     1501 gaatcttttt ttggattcct gataacttgg ataaatcaaa cccgattctt cttttgcttc
     1561 tgaatacagt tttgtcgcat cctcgacatt cttctccatt gtggctggct ttcggaaagt
     1621 aacaattagt ccttttgttt tttctggata cgtacggttc gtacgtgaaa aagcttggat
     1681 caatcctgca tactctagat tacgatccac aaatagggtt tgaatcgtag gtgcgtcaaa
     1741 acctgtcaat agacgatcca ctacgatcac taaatcaatt tgtttcccaa attgtttgaa
     1801 ttccgctctt tttcgtgcca aacgattatt gatgtctccg ttataacgct caatgtctgc
     1861 taatgaccag gaagtatcat aatactgatt atattcctca ataatttttt gcatttcttc
     1921 ttgttgggca cttgaatctc ggctattttc ttctaatgaa taggtaatag cgattcgtgg
     1981 aaaatcagga tcctccatcg ttcgtccttc acgaatgagc tgattgggaa actcttttgt
     2041 taaccaatca ggctctttcg tcatctcttt gatggcccga taatactttt tggccatgtc
     2101 aatggaactc gtggttaaga ttgcagattt cgttggcctg ccatttcgaa aatcaaattt
     2161 tgtataggca ttatctggac ggaacatttt atgaatgact ttttggatat gttcatctgc
     2221 ttcaaaaaca gctgggtcca aataactttc ttttttgaca ggttccattt gatcaatcat
     2281 acgattgatc tgttctgatg aatactctgc atatgtttct acttctttta atttccgata
     2341 gattttattc tctagtgaag tcggctccac ggtgttttca tgctctactt gaaatcctaa
     2401 gacagagcca tcttctagcg cattcttaat ggtataggta tgcaagacct ctccatactg
     2461 atcatgggtt gtacgtgcca attgcccttt ggcttgtttc cgattctcag gaaaaatcgg
     2521 tgtacctgta aagccaaacc aagtggattt cggaaacatt tttttgattt cttccatatt
     2581 ttcagcactt aacgctcgat gacactcatc tactacaaac acgatatgtt ggcccattaa
     2641 tttttggaag cgattggttc cttttttctc ttcttgtttc tttgcatatt tcaacgccgc
     2701 atctagtttt tgacgggttg taatgattac aacatttgca ttggcatctg ccaacagggt
     2761 ttcacttaat tctttggcac ttcccgttcc aacgatcaag ctattcgctt tggcgttccc
     2821 ggaagaaatc ccagtattaa attccgaagc aaattttgta aactctgtgg tggtttgatt
     2881 atctaaatct ttgcggtcca ctaacatgat cgttcgatcg atccccgatt ttcgtgctaa
     2941 aagcttagta gatacaaaac tagtcaacgt ctttcctgaa ccagtcgcat gccacacata
     3001 ccctgattga tgtttatttg cggcaataaa caaggcttga atcgcatgta cttgataagg
     3061 atgtaacacc atgagggttt tattatcttg atcttcactg acgattgtat aatccgcaat
     3121 caaacgatga gcatccggga tatttaacac ttgtttacaa aactcatata agttttctac
     3181 tttttcattg tctttcgttc gccaactaaa taaaaactta gggtgcaagt ctttcggtaa
     3241 ggcatttgca aaataccgag tggtttgttc attggaaacc acaaacaact gtaaggtcga
     3301 aaagatattg ttccgaaaca taccttcttc tgcatatttt ttgatttggt taaatgcctg
     3361 ataaacacca tcttttgcag ttacttgttt cagctcgatt tggacaatcg gtaatccatt
     3421 gattaataag gtgacgtcaa aacgtcgatc ccgtgcttct atgttacttc cacgttttgc
     3481 aatttgatgg accacttcat aagtagagat tcctccacca atgtcttgat tggaataaag
     3541 gataagtgag acagaaccca attgcgaatc ttcccgttca atcgtgatcc gagccatccc
     3601 attttcacct tttaaccatt tcgcagcatc aaatggggtt ttcgttcgca gcagtaattc
     3661 cgttttgatc gtttcaaatt ctttatcggt caaaggaaaa tcattcagtt ctgcttgatt
     3721 atttgagata attttttggc gtaaattgac ccaaaggtct tcttctgatt ttagatccgg
     3781 acgatacgtc cactgattat gcccttctcc aagtacacga atcaactgtt cttcgacttc
     3841 tgcttcctca cggtgactaa ttttcattct tcttccctcc tagacaaaca tcttttgtaa
     3901 gaagcttttt ttcgtttctt ttagtaaatc taacttacgt tgatgaagag cgatcgtatc
     3961 atctagctgt ttgaagaaag taccgatttt ttgttgttca tcctttacag gaaccttaac
     4021 actgatattc tcaatatcag aactgttata agaaggcaat gcccctactt gggagtatct
     4081 tggcaaattt atagtttcaa acaatatttt accaaaatag atatcccaag tattattaaa
     4141 taaataagcc atggtattgt tatctattaa gaaagggctt gtgaccaaac gttttctatt
     4201 aagcattatt gctgcgccaa ctttagcaaa tataatcgct ggcacatctt ttattggctt
     4261 ccatttttta cgctcaattt gctcattaga tacataatga tttgcatttc tcatttcata
     4321 ctcattacca atgttattca tgtcggaaac tttaaaaaat ggagtacctt cagaaccacc
     4381 ttgctcggca tcaggaaatc caattcccga ttgagtttta cctaattctc ccaacttacg
     4441 ctcttcccaa tcttccgtaa aaccaggaaa ccggatttct ggcacttttg ctccgttttc
     4501 agggaacatt ttttgtaaga atcctttttt cgtttccttc agcaaatcta acttacgttg
     4561 atgaagagcg atagtgtcgt cgagtttttt gaagaatatg ccgatttttt gttgttcttt
     4621 taaatcattg ggaatataga atactgtatc tgaaactttt ttccagtctg aacgaggcat
     4681 ttttgttcca gatgtatcat ttgcagcctt ttgatatcta tcagcttgaa taagagaata
     4741 aataaagtcc gagtcgctct tcttagctct aaaaacccag aaatccccaa tcgctacgcc
     4801 tgtaaaagtt gccttgagcc aattttttaa atatggacgt aactttccat atagaatatc
     4861 atttggtaaa aattcaatcc cttttctatt atcaaatttc tggctcacat ctttattcag
     4921 tctaccttct cctgccacga tatcttcaaa ttcaactttt ggaagtctac tggagtttgt
     4981 acttttatta agtctaacaa ctaaatcaat taacttacgc tcttcccaat cttccgtaaa
     5041 tcctgggaaa cggatttctg gtcgtgtatc attgctcatg tttgaacacc tccagcgcac
     5101 cttgtaacca catttcgttt tcaggtgttg tttgcaggga agagatagct tctaaaagtt
     5161 ctttttctag cacttgctgt tcttgtcgta tcttcttgat gtcatttccg acatgaacca
     5221 tatcaattgg ttcttcttct tcgaaagtat ctacatagcg tgggatattt aagttatagt
     5281 cattttcttt gatttcatca aaggttgcca aatgagcgta tttctcgaca tcttttcttt
     5341 cagcgtaggt ttcgaggatt ttttgaatgt tttcttccga tagtttattt tgatttttcc
     5401 ctttaacaaa ttcacgacta gcatcaatga ataatacatc tcgagtttga cgattcttct
     5461 tcaacacaat caccgtcgtt ggaatcgatg tcccaaagaa caagttcgca ggcataccga
     5521 tcacagcata aatactgcca tcttctagta atttttgacg gatgacccct tctgcagcgc
     5581 cacggaataa aactccgtgt ggtaagacga tggccatggt acctgtttcc ttcaaatgat
     5641 agaacccatg aagaagaaac gcaaaatctg ctttggattt tggcgctaat ttcccataac
     5701 gattaaaccg agaatcatct aagaaagtcg tatctgctga ccaattagcc gaataaggtg
     5761 ggttcattac tactgcatca aatgtgtaag gttcatctgt tggccagtcc ttattcaacg
     5821 tatccccatt gcggagattc atttcttcgg cgtcgacacc atgtaaaatc aagttcattt
     5881 ttgctagatt gtatgtcgtt gtatttagtt cttgcccatg atatttaacg ttatctgggt
     5941 gtgttaagta gttacgtacg tttaacatca atgaacctga acccatcgtt ggatcaaaaa
     6001 cactaaagaa tcgtcgttcc ttttgatcga gcgttacgat ttgcgccatc atatcagaaa
     6061 ccatatgagg tgtgtaaaat tctccggctt tcttccctgc ttctgaagca aattgactga
     6121 tcagaaactc atacgcatcc ccgatcacgt ccccatcgtg ttctaaaaca tctacatcat
     6181 tcaatttctt gatgacttcg gtaatcgtca catttcgttg ttgctcatct gttcctagtt
     6241 tttttgattg gagatccaca tcatcaaata acccattaaa ttgattgtag gtagatgcca
     6301 attgcacaaa ggctttgttt agatcgttca attggaacgt tgcttgtttt gcttgatccg
     6361 ctaagacgtt gaaaagatac tctggtgcaa ttgcatatcc caaaatatca acgattgtgg
     6421 caatcagatc ttcttttgat tcttcatctg aaagtaattc acgatacaac attgtttgtt
     6481 tgcttactgt gtcatattcc tctaaggatt catctgctaa aagaaccact tgttccaaca
     6541 atcgatcgga taaatattta tagaaaatca gtcccaataa ataattttta tattctgacg
     6601 catccatttt actacggaga ttatctgccg cac
//
LOCUS       DACDEG010000114         6547 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.119, whole genome shotgun sequence.
ACCESSION   DACDEG010000114 DACDEG010000000
VERSION     DACDEG010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.119"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(23..445)
                     /locus_tag="IV142_002471"
     CDS             complement(23..445)
                     /locus_tag="IV142_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486065.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(508..753)
                     /locus_tag="IV142_002472"
     CDS             complement(508..753)
                     /locus_tag="IV142_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486066.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            898..1737
                     /locus_tag="IV142_002473"
     CDS             898..1737
                     /locus_tag="IV142_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8486067.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            1888..4905
                     /locus_tag="IV142_002474"
     CDS             1888..4905
                     /locus_tag="IV142_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8486068.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            4969..6432
                     /locus_tag="IV142_002475"
     CDS             4969..6432
                     /locus_tag="IV142_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8486069.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
BASE COUNT         2439 a         1118 c         1335 g         1655 t
ORIGIN      
        1 ttttcctctt gctattagaa acttatttca gtgagtaagc aaagtattgt tcatttactt
       61 tagccaaacc aaacgtatcc ccaatcttta acttgttagg ggtatattga atgtcaggaa
      121 tggagaataa attttctttg gtatttgtta ctttgataga aaattcaagg tcagttcctt
      181 ctttaggagt aataaacgca atatttttct tttcaaataa gacagtagta atttgagaag
      241 aaccaagatc aattaaatct tgtccatgcg tttcttttaa actatttagt gaaggataga
      301 cggaatgatc gctagattga cttgtagtag tcgattgaac cgtattttgt ggctgtgttt
      361 ctgttttgtt ggatacagaa aaagcaataa tagcgagtac aatcagtacg attgaaccaa
      421 ctgaaacaat gtatttctta ttcatagaac ctccgttgta aaagtgttta ttattgtttt
      481 acttgtaaat ccaagacaga aaacgcctca ttgacgtttc tgtctttttt cttcgagctg
      541 ttttgcgtat ttttcaatat cttctcttag atataagcga atgatggaag gaccaggttc
      601 gtcgcctatt tctagaaatg gtttgatatt atttctacgt aaagagctag aaaaagcatc
      661 atagttttgt ccagtgattt tggcagcttg tttacgatta agtaaattat tttgagccca
      721 atcatgtatt tcatttaaat tattaagttg catgtgtgac attcctttta gttattttag
      781 taccaaagtt agtatatcaa aaaataaaaa aatctacaaa aaatatatcg actgatagac
      841 ataagtttgg tactatgttt ttgtaaataa atttattcaa atcggaggcg aaaaacaatg
      901 aaaatgttga aagtattggc actaggaatc gtagtcggag gagttagtct tgctgtggga
      961 acgaatgctg aagcagcaga aaacaaagtt tggacacctc gttcggcgga acaaatcaaa
     1021 gcagaaatta aagggaatga atacacaatt gtctggggag ataccctaag cgcaatcagt
     1081 caagcaacaa atattactgt ccaaaaatta gcggacatga ataagattac aaatgtggat
     1141 ttaatctatg caggaaataa attagtattt aatggcaatg tagcaagtgt acaaaacagc
     1201 gcaggtgaaa ctgtggctca aacagtgatt caagagaaag acaaagttga tccgaatcaa
     1261 ccagtaggac aaggaacgac ttcaaataca caaacgacac caaatacaac gacaccaaca
     1321 acaccagctg gagatcaaac aggaacaact ggtggtacaa caacgccaag tacaccagaa
     1381 aacaatggtg gcactactca aacacctaca caaccaggta atggtggaaa tactggagga
     1441 gaaacaactc cttctaaccc aagcacaggt ggaggaacaa caacgcctga agaaccaagc
     1501 aatcctggtg gaggcgatca aggcggaaat ggtggcacaa cgacacctga agagccaaca
     1561 attgtagttg gagagattgg taatagtgga aaggtattta acactgtaga tgaagcctat
     1621 gattgggcta atgcagaggt tagcgatcca aattcgcagt ggtacaacaa aggttttatg
     1681 gtggatgtta ttatttacag tgacgggtca caaaaacaca ctgtagattt ctactaaaaa
     1741 taaaagaaaa gtaagtggtt gatgaagact agaattataa gttctagtct ttttttgttt
     1801 tgaggaggat aaaaatgttt cggtgtcgaa gtccataaga aattacttta gaagactgaa
     1861 aaataaaatg aaaggaacga aaaaaatatg tttaaaagaa tggcaacttg gttggcaatt
     1921 ttcgttacat ttctcaatcc catgttagga tctgtaacgg catttgcaga aacgataaat
     1981 gaatcaaatt ctactgaaga agtaatgaag actgaagaat caaaaagtag tgaagaagcg
     2041 atcagtactg aaaccacaag tgaaacaaca gaagagacaa ctacgcagga cacgacagaa
     2101 gatgtttctc cgcccaatgt aatgactcaa gaggtccctg aaggttatga gaaatacttt
     2161 ataaccattg aagatattca aaaagcgact tctttttctg aacaagaacg attggcgcaa
     2221 gaatctgaag aatggcaagc aatcttggaa aagaatgcac agacacgtgc taccaatcta
     2281 acattgactg aaaatggtcg taaagatgtg aatatgactc gaattactcc tgaaggaaca
     2341 atgtatactc atgattacat ggttcgctgg caaattgacg gagaagatgt tttttgcatt
     2401 caagaaggcg caatcacgag tgcgggtatt caatatgatg tgacaccact cgaaaattta
     2461 attacgacaa gtgctattag aaaaaagatt tctttaattg gttattttgg ctactattct
     2521 agtggttcaa tggataacta tgtattagca caaacatata tttgggaagt tttaggtggt
     2581 tcatttgtta attatggaac tattggacaa tctaattatg aagctttcaa acagactgta
     2641 gatagaaaaa taaacaattt acttgaagat ccttcttttg atagccaaac agtgacggtt
     2701 aaagtagggg aagaagtaga agttaaggat acaaaaggtg tgttttctca atgggatggg
     2761 tcggttgttg aaaacacagc taacgttcaa gtaagaaaat caggaaatag tttgtttata
     2821 aaagcaaatc aaaactcaaa tgacggacga atttctgttt cttcactgga tccaaattct
     2881 agaggggtag gttcaagttt tgcctatgta gatcctgttg ctcaaaatct aggaaagctt
     2941 tatgtgccag atcctaatag agctggacta actattaaag ttctaaaaaa tggcgacttc
     3001 aaactaacca aactagacgc tgtcacaggg aaaaaagttc caaatgtgac gtatgaaatc
     3061 aaacgtcatg atacgggaga aacaaaacaa tatacaacgg atgccaaagg ggaaatcgcc
     3121 ttaaaagaaa tccctgcatt ctatgaaaaa gacggaaaat atgttcaaaa taaattggat
     3181 gtcaaagaag tcaaagcacc agctgggtat attctagaca aacaaactca tacgattacg
     3241 attgaaccaa acaaagaagt gacgttaact gcaaaaaatg acttacaaca gttcattcca
     3301 gaaggaaaga aagtcaaaga agtctataat gcggaagaat cctggaatca aggcaaacct
     3361 gtttatgata ccgttcctgc agaaaatatc gaatttgata ttatcgccaa aacagatatt
     3421 actgcgccag ataaagaaac agtccttgtg aaaaaaggag aagtcttgga ttcagtaaaa
     3481 actgacaaag acggacactg gaaggcaaac aaaaaattct acaatggcga tcaaaacaaa
     3541 taccaattga ttgaaaaaaa tgtgccagac aactaccgtc aaccttctga agaacaaacc
     3601 acgttctctg tggcctatgg cgataacacg acgcctgtgc ttacgtttga attagatgtg
     3661 attgacaact tgttaaaaac gggtgaagta gacttcaata aagtcgattc aatcaatcat
     3721 ctacaattac caggagcgaa attcctgatt gaaggtatga ctgcacataa taaaaatgtg
     3781 tcctttgtgt atgaaacaaa agaagaacca gaaaaactaa aattaccaga cggtacgtat
     3841 aaatttacgg aaattgtgtt ccctgagcat tatgggcaag cgtcaggaca aacagaaacg
     3901 attttagtaa cggtcaaaga cggagaagta caaccaatta ctgtgaaaaa cgaagaaatc
     3961 gtggttccag aagaagacca accagaaatt tctactttgt ttgtgacaac gacaggtgga
     4021 aaagatttca acccgaaaaa agaccaagaa ttagtggata aagtgtccca agtattccct
     4081 aaatctgcgg ttgggacaga gaaatattgg gtgacgcaat tccataaaat tactgccgat
     4141 ggaaaagtca ctgtattaga tacggtaaaa gaaacacgaa aagtgaccga tgaaaaagaa
     4201 acatttgatg tgacgttcaa ctacaaagca gacatggttt cagacggtga cacgattgtc
     4261 gcaacgcaca ttgtctataa cgacaaagaa gaggaagaag aattcgctcg tcattttgat
     4321 ttaaccaata aagatcaaac agtgactgcg aaagaaccaa aaatcgaaac acgctttatt
     4381 acagtgaatg gcgaccaaac atttgatcca gacaaagacc aagagttgat cgatcatgta
     4441 tcgcaagaat ttccagaatc tacggtaggg gaaaccaaat actgggtgac tcaattccat
     4501 aagattgata aaaatggaaa agatacagtc gttggtacgg tagaaactgc acgtaaagtt
     4561 tctaaagcgt ctgaagagtt taaagtatcc tttgactaca aagcaggcac gttaaaagac
     4621 ggggagaaat tggtcgcaac tcatattgtc tataaggata aagaccataa agaggaagaa
     4681 gcacgtcact ttgatttgaa gaatgaaaaa caaacattga cagcaaaagg cattaaaaaa
     4741 gaaacgccga caacccctgt caaacaaacg ccgacgagca caccaaaaca aagcttacca
     4801 aaaacaggag aaacagtgaa cgatgtgtta attgcattgg gactgtttct ttttgttggt
     4861 gtgggggctg ttctggttcg tcgtaaaatg aaacaaagca attaaagtat gactagagac
     4921 agcaatcagc tgtctctaga tgattgattg aagaatggag gcaatcttgt gaaaaaaacg
     4981 agtaaatgtt tatctgcttt gttattagta ggtcaagtat ttgggtatac gccagcggtt
     5041 ttggctaatg aacaaggcaa caatcaactg acaaattcgc aatctgcaac gatcactgaa
     5101 gaaacgacaa caaattccac tggagaagaa aatcaaccag aaacaccgac tgaagataca
     5161 atagatacac cagataatcc agaaatgaac gctccaccaa caggttctga agaacaacca
     5221 gaaacaccga atcaaccaga caaaccagaa gacaatcaac aacctgagga ggaaccgtca
     5281 gaagaaccaa atgagcaacc ttctgatgaa agctcacaga ctcctcaaaa accaagtacc
     5341 gaaacagaag cggaaccgcc aacaaaaacg gaacgcccac aaaaggcacc tgaacagagt
     5401 gtgactgtac ctatgaatga cgtaccgcag gcacaatcaa tcggttcaga agagacaaaa
     5461 aactaccacc aagcaccaag cgcaccgata caagaattag tcccttccag cacacaagat
     5521 aacgaaggga ctattcattt tgaaaaagac gcgtctgtag aaacgtttat ccgtaaaatc
     5581 ggtgaatctg ctcgtaaaat tggacaagaa aatgatttat acgcttctgt tatgatcgcg
     5641 caagccattt tagaatccgc aagcggacaa agtcaattgg ctcaggcacc aaactacaat
     5701 ctatttggaa tcaaaggcac acataacgga aagggcgtct cttttgcgac acaagaagat
     5761 ttaggcaatg ggacacttta tacgacccaa gcaacttttc ggcaatatga gagctatgaa
     5821 gaaagcttaa aagactatgc caaactgtta aaagaaggct tagttggaaa tacctctttt
     5881 tatgatggtg tttggaaaag taaggcaaaa acctatcagg aagcgactaa atttttaaca
     5941 ggaagatacg caacagacac tagctacgat aaaaaattaa atgggttaat cgaaacctat
     6001 gacttaatca aatatgataa agaagtagct ggtccacaac taagcaaaga aggctatatg
     6061 gtacctctaa aaaattattc aatttctagt tccttcggtt ggcgaggaga agaatttcat
     6121 agaggattag acatggcagc caatcaagga gaaccaatct atgcgagcaa ggcaggaacg
     6181 gtcatagaag cttcgtatca tactttgtca ggaaattatg tcacaattga gcatgaagac
     6241 ggaagtgtgg ctctttatgc gcaccaacag gaatattgcg tgaacgtagg acaaacagtt
     6301 gaacaaggac aaattattgg ttatgtgggt tcaacaggaa atagtacagg accacattta
     6361 catttagaac ttgccttaga tggtagttta acaaaagaca aattaatcaa cccagaaaca
     6421 gtcttattct aaagaataag gctgtttctg ataaggagtg gtatagaaaa tgtggttact
     6481 ttttaaatat ttgatatatt tgtatctctc attttcgttt tttaatttga aatctcgtat
     6541 ctatcta
//
LOCUS       DACDEG010000115         6467 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.120, whole genome shotgun sequence.
ACCESSION   DACDEG010000115 DACDEG010000000
VERSION     DACDEG010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.120"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            25..669
                     /locus_tag="IV142_002476"
     CDS             25..669
                     /locus_tag="IV142_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486070.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPVDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            680..1486
                     /locus_tag="IV142_002477"
     CDS             680..1486
                     /locus_tag="IV142_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486071.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVAEDQENTENKINSYQQEIETSKTQMKDQEESIQKLQEKIDRMTKENI"
     gene            1509..1738
                     /locus_tag="IV142_002478"
                     /pseudo
     CDS             1509..1738
                     /locus_tag="IV142_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1897..2832
                     /locus_tag="IV142_002479"
     CDS             1897..2832
                     /locus_tag="IV142_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HAP8486072.1"
                     /translation="MSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIE
                     YTWLPLKRNSKRKEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQI
                     AASNIFTAVMTLMCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDR
                     FEQSEKTVEGIKNFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDL
                     IPTLSGYEYYVDFGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYN
                     FYKVEASLRYGIGKLRLDGSNIQQNKPYVLGKVVI"
     gene            2876..3145
                     /locus_tag="IV142_002480"
     CDS             2876..3145
                     /locus_tag="IV142_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486073.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            3422..3700
                     /locus_tag="IV142_002481"
     CDS             3422..3700
                     /locus_tag="IV142_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486074.1"
                     /translation="MEVKIRDIDEKTIQRIDRRCIELSERTGTKWSRNRYLKVLIEND
                     FDHALLDYKKDQFDRLLEKFVDIQTYNTKILEEYIATNNQLIALLIEQ"
     gene            3739..4161
                     /locus_tag="IV142_002482"
     CDS             3739..4161
                     /locus_tag="IV142_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486075.1"
                     /translation="MAKATRKHIYLYDMHIEVLDEIISTHDDISNYSEAVRHLCMMYD
                     PKTEEKISPSQIKLNAIGKEVSILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            4181..5773
                     /locus_tag="IV142_002483"
     CDS             4181..5773
                     /locus_tag="IV142_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486076.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTLEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEYGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTEREKIEYQRLNQAMEKYQAEPHKKTSGTLPGVFSISSDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEVGLKAAGRK
                     MMETLFEKEKLNDTGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNSLTKVVKEPVRYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDENRTKLSPYTREKLDELTQYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QTQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            5803..6138
                     /locus_tag="IV142_002484"
     CDS             5803..6138
                     /locus_tag="IV142_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486077.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLINEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            6279..>6467
                     /locus_tag="IV142_002485"
     CDS             6279..>6467
                     /locus_tag="IV142_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486078.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYPF"
BASE COUNT         2535 a          920 c         1292 g         1720 t
ORIGIN      
        1 aaaaaataga tcaggagaaa aaatatggaa aataaaacct taaaaatcgt cttaacagct
       61 gcaattagcg ttagtttaat cggcaatatt gcgttgtttt ggcagcttaa agaagaacaa
      121 gagacaaata cctataaaat tgaagaagtg gctaaaaacg cgtctgataa gctcaaaaaa
      181 aattcttctg aagcacaggc gaagattgac aagttaaatc aaaccatcaa tgagaaaaac
      241 caagaaatcg atcagctgaa gcaacaagat ggcacaaaag caaaagagga agtaactgaa
      301 gcacaaagaa agacggcaga agagttcggt caattggcca ttgataaatc attaagcaga
      361 aatgaattaa cggaaaaact aaaagatgtc gcaacccaag aagtgatcga caaattaagt
      421 cctgtagacg atgatcacca acatgatgat tacggaagtt actcttttac attaggtgac
      481 gtgcaaactt acgctcaaac aagcagtgta aaagaggaac aaaattttgt cgtatttgtt
      541 gattatacaa tcggcaatcc tcaattcaaa gatgtcaaac cacaaaaaat aaaaggtgga
      601 ttaactgtca ctgaaaaaca agtagatggt caatggaaag tcacagactt tagttatttc
      661 actcgttagg aggaagaaaa tgagtaagaa aaaaggagca agacaacgtt ttaaaaaacg
      721 aaaaagttat cttagatggc tggggatctt tatgattctc ggctttttga ttcttgctag
      781 tccgatttac acacataaaa aggtagacat accagatgaa gtaagcacga acgcattttt
      841 aaacacgaag gaactgacga tggtttcaaa agaaaaagga gcgaaccagc aattagtttt
      901 tgaatttgcg atcgacgatg aagatcagag cttattaaaa gaactagcta atcttaatta
      961 tcgtgtagaa gtaaagacag ctaaagggga ttatcaagca ataaaagcca aagtgatcaa
     1021 ggtgtctgat gattattttg tagtgaagtt agcacatgta ccagaagaat atattgctat
     1081 gcgattaact attattcctg aaaaaattga tccaaaggtg gatatgcaag aaccgcaaga
     1141 tctgattttt tatattcacg aagataaggt caaagatcac gtgaaagacg aagattatga
     1201 acaacatgcg atcaattaca aagtgaaagg atacaagaaa gaacaaaaag cagcacaaaa
     1261 acaaattgaa tccttccaag caaccattga tttgaatgaa gaattagtga agaaactaaa
     1321 agatcaattg ccttatcaag tcgcagaaga tcaggaaaat actgaaaata agattaatag
     1381 ttaccagcaa gaaattgaaa catcgaaaac gcaaatgaaa gatcaagaag agagcattca
     1441 aaaacttcaa gaaaaaattg atcgtatgac aaaagaaaat atttagaaga ataggtggag
     1501 taaaagaaat gaagcgagcg aacttggtcg gcaaagatac tttcgctgag cagaaagtat
     1561 tgattaaagc ctatctcaaa gttgtaaaag ctaaagaaat atgtcaagaa gtgaattatc
     1621 taacacaaca atatctgagt tgaagaatgg tagaaagagt ttagatacct taaaattcag
     1681 atcatttgaa gctctatata tagctgctaa aaaatatttt acagctgata ataaataaaa
     1741 aattaaggtg ggttctatgt gaaaaaaacg tatcaaaagt ttttatggat tagctatatc
     1801 gtcctattta tcgttgggtt tctcgtcagt gtgtccgtga tcgtcggaac gctttttctt
     1861 ttgttctctg acgaagatct ggtacttaag gggttaatga gtacgttatc gtgtgtcatg
     1921 ttactttatt tatgtataca gatcaacgaa attatgcccc ctcattggca agaaaagaag
     1981 aaagaattta aggaagaaat cgaaataaaa tccaatgact ggattgaata cacatggctt
     2041 cctctgaaaa gaaatagcaa acgaaaggaa ttttgggcat tgcaattatg gccaatagga
     2101 aaacttttta tttatatggc ttcacttatg gttactttaa tcggttattt ggtggtggtc
     2161 tatggggtgg cctattactt gttaagtgag ccaaaacaga tcgcggcgtc aaatattttc
     2221 acggcagtaa tgacattaat gtgtgtcatg tgcttttcta tttttagtta cttactcgtc
     2281 caatttatca ggcaaaattt aacaatgagt aaacttttca aaagtttatt ggacttactt
     2341 atcttaggca gtatgttaac gactctaaca gatcggttcg aacaatctga aaaaacggta
     2401 gaggggataa agaacttctt aacgacgatt gatcaattta taaatggata tatgtatttg
     2461 tttgaagtag gctttattct cactttgttt ggttgtgtat ttataaaact cattaaaaga
     2521 tcaatcgaaa aagaaatggt tattgatcta attccgacgc tgagcggcta tgagtattac
     2581 gttgattttg gaaagtatga ggagttgatc catacggata tcgttcacac gagtatccat
     2641 gaagtaaccg ttaagggcat cagatctact ttaaaagatg gacaggagac gaaaacaatc
     2701 gaccgaagaa aagaggtcta caatttttat aaagttgaag caagtctacg atatggcatt
     2761 gggaaattga ggttagatgg gagtaatatt caacaaaaca aaccatatgt tttaggaaaa
     2821 gtggtgattt agaacttcct atgaaagatg aataaataag gagaatcttt tgaaaatgat
     2881 tcttctttac agaattgtgc ttatagttgg cacggagtta aaaatcttaa agaaggagca
     2941 gttattcaaa gtgaaagaac atcataatca gttactatgt attaaaacgg gtacgatgaa
     3001 cattcaatac cgaacagaat atttagaatt aacgaatcaa actgattttt tgtcgattcg
     3061 agagcaacat aagcaagaaa aattaatggc tacttatagc caattgattg atgagtggga
     3121 acaattcaag gaatctaaaa cataaaattc catttaaaca ccttattttt taaagagcat
     3181 aaggaaacgt gtacccactt tcaaaataaa gtgggtagat aggctgtgcc tatcacgttt
     3241 ctgaaaaaaa taaatgaccc caaaattttc aatttatgtg taggtttatg ggtagcaaaa
     3301 atatgaatgt gggtagcaca aaagttcatt tgtgggtagt aaaatttaaa tatgtgtagc
     3361 aatgtgtgta gctacacaca ttgctacaca cgttataaca tatagtcaaa aggagatatt
     3421 tatggaagta aaaataagag atattgatga aaaaacgatt caaagaattg accgaagatg
     3481 tattgaatta agtgaacgta ctgggactaa atggagccga aatcgatacc taaaagtttt
     3541 gatcgaaaat gattttgatc acgctcttct ggattataaa aaagatcaat ttgatcggtt
     3601 gttagaaaaa tttgtggata ttcaaactta taatacgaaa attttagaag aatatattgc
     3661 aacaaataat caattaatcg ccttgttaat tgaacagtaa aaataatatt gtgaatttat
     3721 aaaaggagga aatcaacttt ggcgaaagca acaagaaaac atatttatct ttatgatatg
     3781 catatcgaag tacttgatga gataatttct actcatgacg atatatccaa ttatagtgaa
     3841 gcagttagac atttgtgtat gatgtatgat cccaaaactg aagaaaagat aagtcccagt
     3901 caaatcaaac taaatgcaat tggaaaagaa gtttctattt taactgaaat ggtttcggag
     3961 tttgcagatc ttcatttaaa agatacagga atattaattg gtacaaattc aactgtttat
     4021 caagaagcga aagagcgtgt ggaaggcaag ataaaagcaa atcaaacgaa gaaatattct
     4081 cctaagataa agaaaacaat aagcgaatca gtgactacaa aaagtgaagt atttgatcca
     4141 acaaggtttt taaaacgata aaaattagaa ggagaacgtg atgagtaaat cacctgcagt
     4201 cattctcact tgtcaattta ccttgcccaa taaaaaatct ttttcaacat acattgatta
     4261 tatgacaaga gaaaaagcgc tggaagaaat ggaacggcgc acgctcgaag aagaacagga
     4321 actgcaacga atcaagaatg ctttagagga tttttatatt cctgaaggag aaactttttc
     4381 ggagagtaag acggataaag aattcagtga aacgtcgaat gaagcgcaat cgttgatgga
     4441 atatggaatc aattttgaca agttagagga acaagatttt accaaatatc tttcttatat
     4501 gacgcgtcac tatgcgttag aacaaaaaaa ggaactaacg gaaagggaaa aaatagaata
     4561 tcaacgtttg aatcaagcaa tggaaaaata tcaagcagaa ccacacaaaa aaacttcagg
     4621 aactcttcct ggagtttttt ctatatcttc agacgaagta aaaggaagtg atctgaagga
     4681 gattaagcag atcttccaaa gagggcaaca aaaaggctca atcatttatc aagatgttgt
     4741 ttctcatgat aattcgtatc ttgaaaaaat aggattgtac gatcccaaaa cagatacttt
     4801 agatgaagtt ggactaaaag ctgctggaag gaaaatgatg gagacgttgt ttgagaaaga
     4861 aaaactgaat gatacgggat attggatggc taccattcac cgtaacacta agcatattca
     4921 tattcatttt gcagttgttg aaaaagaaaa tacgcgtaaa ccatacactg aggtcgaaaa
     4981 tggtgtgagt tatatcgtac cgaaaggaaa gagaagacaa gcaacattag acgcaatgaa
     5041 gaccaattat gtgcatgaat tagaacgttt ttcttatgaa aaggatcgaa tgataggcat
     5101 ggagaaacga aatttattaa cgcggaaaag tgatttgcgt aacagcttaa caaaagtggt
     5161 aaaagagcca gtacgttatg atcgacacgc aatgaactta ttaaatgaag tatataaaac
     5221 cttacctgac aaacgttcgg aatggaatta tggggatgaa aatcgaacta aactttcacc
     5281 atacacaaga gagaaactcg acgagttaac ccaatatgtg ttaacaaaag aacctgaata
     5341 ccaagaatat atacagattt caaaagcatt gaaagaagaa gcaaaaagtc tttatgggga
     5401 aagcaagaga gaatcaaagg atgcagagaa aaatgctctc tttgatttaa aaaagcgcac
     5461 aggaaatgcc atcctaacag agctgaagag acaggatagt caaataaaac cattaataga
     5521 aagtgttgat cagacgcaat ctatttacaa tgaacgatcc tttcgaacgc ttaaacaatt
     5581 gccaacgaat gaatacaaat atagttacga agaattcatg aagcgcagga aacaattcca
     5641 acaaagagtt gtcacgcagc ggagtttgcg tcaattaaat cgaacgatta atgcccatga
     5701 agaaaaatac cacgcaagta aagcatacga agagcaacaa cgaagaatcc aaatggaaca
     5761 agaaagacag taaagacagt aaagacagta ggaggaatag caatgaaaca atatttagat
     5821 caatggaaag ttatagaagg atcattaaga gaagaacgaa tcgaacaact tcccgattgt
     5881 ttagaaaaag agcatttgtt tcagataaga gaaatgttga taaacgaaca gtttgatcca
     5941 aatcaatttt tagtcgttga gtacccagct actggtgtgt actgttgtaa tcatgtgaag
     6001 ggtgaaaaat attttatcat tcaagaatat gagggcaagt tagcgcctta ttatactact
     6061 tgggaaatga atgaagaggg gatcaataac ttcccgtgta aaagcattga agagtctatt
     6121 tcactcacag aatgttagtt gttaagtgaa taaaagaagt aaaagagtcc tgaacgtgat
     6181 tgtccaggac tctttttttt cactgaattg ttttccgcca attcattgag tggtgcgatt
     6241 tccgtctaca ttatatcaaa agaaaggagg aagaagaaat ggcttattca acgattggag
     6301 ttgttgttga aaaaagcaga gacaatcttg tttttgtgac agaaattcaa acaggcagag
     6361 cttttgtagt cacagacaaa gcggcaaaag catatcaaag tggcgatatt ttaacgttaa
     6421 atctgactac gaagacgttc gtcgatgcgg cagaagatta tccattt
//
LOCUS       DACDEG010000116         6316 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.121, whole genome shotgun sequence.
ACCESSION   DACDEG010000116 DACDEG010000000
VERSION     DACDEG010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.121"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IV142_002486"
     CDS             complement(99..1229)
                     /locus_tag="IV142_002486"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP8486079.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IV142_002487"
     CDS             complement(1878..3080)
                     /locus_tag="IV142_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8486080.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IV142_002488"
     CDS             complement(3140..3343)
                     /locus_tag="IV142_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8486081.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IV142_002489"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IV142_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IV142_002490"
     CDS             4880..5692
                     /locus_tag="IV142_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8486082.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1875 a         1299 c          996 g         2146 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctct
//
LOCUS       DACDEG010000117         6087 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.122, whole genome shotgun sequence.
ACCESSION   DACDEG010000117 DACDEG010000000
VERSION     DACDEG010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.122"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..502)
                     /locus_tag="IV142_002491"
     CDS             complement(<1..502)
                     /locus_tag="IV142_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8486083.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEF"
     gene            1133..1228
                     /locus_tag="IV142_002492"
     CDS             1133..1228
                     /locus_tag="IV142_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP8486084.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            1928..2209
                     /locus_tag="IV142_002493"
     CDS             1928..2209
                     /locus_tag="IV142_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486085.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            2211..2627
                     /locus_tag="IV142_002494"
     CDS             2211..2627
                     /locus_tag="IV142_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486086.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            3249..4403
                     /locus_tag="IV142_002495"
     CDS             3249..4403
                     /locus_tag="IV142_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAP8486087.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            4397..4819
                     /locus_tag="IV142_002496"
     CDS             4397..4819
                     /locus_tag="IV142_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486088.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            5989..>6087
                     /locus_tag="IV142_002497"
     CDS             5989..>6087
                     /locus_tag="IV142_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8486089.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVS"
BASE COUNT         2144 a         1092 c          984 g         1867 t
ORIGIN      
        1 taaactcttc aatacgaact acataataac cagtagaacg gtactgcata agcagtcgga
       61 atagagtttt tgcatacgta gaacgcaacg acgtgaaagc agataattca aatctactaa
      121 attcagctgt taaaccatta ataatatatt ctaggtccgg attaacactg acttcaacaa
      181 acttttttgt tttattgatt tcaaaaccag taaacaaaac aaagtagcga atagaatcct
      241 catcttcagt acggtaagta agattaagca tatctttata gagcttttct aaatcaccga
      301 caaaggcttt tgtagcagtc atcttataat cacttaactc ttttaattcc tcgaaagaaa
      361 atctaacttt tttcaaacca cgatctttca tacgagcgca taatgcaaaa aataaatcca
      421 tctgcttagc attgaaattt ttcagcggta ctaaattcaa ttcattttga tatttgactg
      481 ttaattccgt tttttcatcc attcctttct tcccccctta caaataaata ataaccttta
      541 acaaccaact cgtaaatagg ttggttgtta ttatacaaaa ggatattagt actcagataa
      601 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
      661 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
      721 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
      781 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
      841 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
      901 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
      961 agccgttcta agacaagatt cgataaaaac tatgtccttc gttctaaagc gccttacagg
     1021 cggtcagggc aagccctaac aaccccaagg caataaaaaa cttaggctta tttttgcttt
     1081 tttattgtat aattgattgt cgcctatccg acagtcttgc gactggaagg aggtgtgttt
     1141 cgtgttcact aactttattt gctcccttct tgttgggctt ttaagcgctt tgttcagtta
     1201 ctggttgaat aaccgaaaca agaagtaaaa cttaattaag gcgactcagc ccacaaaaaa
     1261 agccccagag aatccgacct ctctggggat ttcgtgtgtt tcgtgttcac ttatttgcaa
     1321 tcaaattata acagaaataa atatttattt caaaaatatc ctacaaaagt ctaaaatccg
     1381 taattatttc ttatatgcaa catttacaaa taaagtcgct ctgagagctc gtatttttcg
     1441 atttaagacg ttttatttag tttttggtgt atttcattgg tccaagctaa aaaactctta
     1501 gaagcgattt tagagccatt taaacccact taattatatt ctccatgaaa ttcgctccat
     1561 gcgtcaaaag ctgcgtctga aattccgccg tcttcgttca ctagttgcat taatttttcc
     1621 aacgatcgtt ttctttcggg tgtcatattt ttatattcca acaatttttc gtctgttaaa
     1681 tttggttatc gattacacgt gcagcactcc cccccgaatt ttaaagagca taaggaaacg
     1741 tggtatcact ttcttacgaa aggtgctaga taggctgtgc ctatcacgtt tctcagaaaa
     1801 aaatttgcta tcactttttt gccattgtgg ctaggctatg tatagtgttt tggcgaaaaa
     1861 tagctataca aatactatat ttgtatggta tacataaatt atacgctgat acgaaaggaa
     1921 aatttccatg gacattttag ttcgaaatat caatcccaaa attattgcaa gaatagataa
     1981 acgttgtcag gaactcacaa ccaaaacggg caaaaaatgg agtcgaaata aatatctaaa
     2041 agttctaatc gaaagtgatt ttgaccgtca acttatgaat tacaaaaaag atcaattcga
     2101 tttattactc gaaaaattta ttgccattca aacagaaaat acgaacatac tgaatgacta
     2161 tgttcacaca aacaaccaac tcattgaaac acttatcgga ggaatataac atgagaaaaa
     2221 cttctgttca aaaacacgta accctttatc ccagtcacgt tgacgtttta gattccatca
     2281 tcgaaaaaaa tccagacgtc aacaattatt cagaagccat tcgtttttta tgtatgagtt
     2341 atgaacctga taccaataca accacaacca gtaaccaagc aaaactcaat gccatgggaa
     2401 aagaaatttc tatcatgaca gaaatcatgg cagaatttgc tgatttatat ctaaaagata
     2461 ctggtgttct tatcggtaca aattctaaag tgtatcaaga agtgaaagaa aaaattgaaa
     2521 atcaactcaa tccttcccga cgaaatcaac ccaaaaatcg tcaaaaagaa accaatcaaa
     2581 cctttacttc tggaaatttt gacccaacaa actttctaaa aagataaaaa aagaaaccga
     2641 tccctaaaaa aattcctcga tttcttttta gggatcagtc ttccagcaag atttgcaagt
     2701 tggtaccaac ttgcgaaaaa aaagaaaaat ccgcttatgc aacttcttct ttttgtattt
     2761 tgaggcgcta ccccaatccc caagtatcgg ttatttaata gcgcggaaat ccaaagaata
     2821 ggacgtaccc tgaaacaaaa aaagactgct ctcacgagca gcccaaaatg agattatcct
     2881 attcatcaac ataaaaatat tacaaagtga agaactagag aaagacaaaa aaagcatatc
     2941 aaatcctctc aaaaataaca agtctttaaa aaatgacaga taaaaatgca caaagaacta
     3001 aaaaagagaa atcgcgtaaa atcaagcatt agaagctagt aactagctag accaattaaa
     3061 aatcacaaag tgagcgtctc tatccattgg tagaggggct acttggcata gtgtacatta
     3121 tgttaactaa cgctataaat aacgattatg ttcccaaaac aatattttat gaactataac
     3181 aaaaatttgt tatcgcatat tgtatatttt aattataggt gtataccact taaatttaac
     3241 acaaaggcgt gattattttg aaacccaaaa agaaaattta tctaggtctc ctcttttcct
     3301 tgatgattgg aatttttagt ataggaatta caaataccta tgcttcagaa aattctaatt
     3361 tcaatatcaa ctcccaacca attgaagaac aagcaattaa ttttgcgaaa actgattttg
     3421 aacactccat aacttcaatt cttttaatgg aaaataacac cgaaactgat attaactata
     3481 ctcttggttc gccatttaaa atgctcgatg gagaaaatac ggcaaatagt tctattttct
     3541 ttccagtatt ttcagacgct gataatacga ttaaatatat ttataccgtc aaaaaaaata
     3601 caaatggaga atattcagga agtatttcac aatttttagc aaaagaactt caaaatctag
     3661 tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt tattattccg
     3721 ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa aatggtgttt
     3781 cttcctttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa attaaaaatt
     3841 tctctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc tccggagata
     3901 actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg tggtgtgctg
     3961 cttacgtaga agctgctatc ttatgtaaca aaacagatgt acgtgctact tcagctagag
     4021 ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca aaaagtctta
     4081 atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa aaagtagcac
     4141 gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc atttatatgg
     4201 gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta tttgggtggg
     4261 tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac tacccaactg
     4321 taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt tttacttggg
     4381 acaataccat tacaaattgg taaataaacc cattgcttta gttatgggtc tagtgttagt
     4441 tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc tgattccttt
     4501 tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta atttaaatac
     4561 ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata aaattcaaat
     4621 cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa caaataattt
     4681 actaacactg cgctctaata aaaaacaatt agattacact tttagtttgg attctccaaa
     4741 aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta aaccaattga
     4801 attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa tttcaccgaa
     4861 ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt tttgtattgt
     4921 ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgaa aagcggttat
     4981 ttgcaccaaa tacgtaggta tttggctagc aaatatagag cttgccaaac actttagtga
     5041 gttttgtaga aagtaccttt aaaggtactt tcacattacg aacgatacgt ccgtaggacg
     5101 tactgaaagg gtgggattta tgagcgaact aatgcaaaat ttggctagca aaaaaagtta
     5161 aaaaatatat gctaataatc gagcgaaatg aacacttttt caagtgagcg aatccgccat
     5221 gacaaagctg aagcagttta tttcagcttt gtcatggcgt gtgcctagac ttttaaaaaa
     5281 gtcggcacac gagcaaatat ctttatcaag ctctaatttg cgttttaaga cgtttgaaac
     5341 gtctaagtat ataaatatat aagagtaggt ttaagagtgc ttaaatcgaa ttttagaggg
     5401 cattcagaca actttagggt tgttagggct tgccctgacc gcctgtaagg cgctttaaga
     5461 acaagataag ggatttatac taagactgtc ttaaaacgtc tcaaacgaag gctcatagct
     5521 tctcaaagag ttccttatga acatttttag caaaggagcc ggaagcgact gcgcagtata
     5581 atgtgagagc agagcgatca caccttttta agcgactgta gggagtaaga ttcccagttg
     5641 agaaaaatgc gcgaatctgt ttggagaaaa aagaagtggt tttttaggcg ggaaattttg
     5701 tccccctctt ttataatctc tttttaaacc tctttttaaa cctctttacc gtcggctata
     5761 tccttactct cccaagggaa tgacgctttt atctacatcg ttttgtctgt ttatctacat
     5821 cgttttgtct gtttatctac atcgttttgt ctgtttatct acatcgtttt gtctgtttgt
     5881 acattgaatt agtacatcga cgatgttaat ataagatgta gataaacagg cattttgtat
     5941 ttcataacgt gtatctcttg tgcgagtaag aaaggagcta ttttaaatat gtctaatgaa
     6001 atcgtaaagt atcatcatga attaaataca attccgttgc gaaaatttac accagtcgaa
     6061 atgaatttgt tcttttctat cgtatca
//
LOCUS       DACDEG010000118         6033 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.123, whole genome shotgun sequence.
ACCESSION   DACDEG010000118 DACDEG010000000
VERSION     DACDEG010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.123"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..510
                     /locus_tag="IV142_002498"
     CDS             <1..510
                     /locus_tag="IV142_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486090.1"
                     /translation="IISSEQLDNQGHEKQEGVNGAKFSVYDVSDILQKMDVKDLTTDQ
                     IESQLKNRVKKLSSDQLKLVSNGETKTIDQQTGVFEFSVEVQANQKQAYYIVNESSPE
                     NISNSEDILLLTPVSDKNGEFLKDVWIYPKSEASQPKEEVKKIVSTGVKKNFFENCWD
                     FVTHLFSRD"
     gene            523..783
                     /locus_tag="IV142_002499"
     CDS             523..783
                     /locus_tag="IV142_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486091.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            780..2870
                     /locus_tag="IV142_002500"
     CDS             780..2870
                     /locus_tag="IV142_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8486092.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLNNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTILAVSLLL
                     LVLATFGYAVLKFN"
     gene            complement(2906..4963)
                     /locus_tag="IV142_002501"
     CDS             complement(2906..4963)
                     /locus_tag="IV142_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP8486093.1"
                     /translation="MKLLNPKTVIAIGTFAATVALAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVTSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNTLDSAYKGLKKKPAPTVNKTELQALYNQVKSTAKGDFTEGSWNNFQTAL
                     SNAKKVLDDSKASQTQVNNAKNSLDSAFKGLQHKPKPSEYAVTVRHMNRQTGTLLKEE
                     HVSVKAGTSYTAKARTDLTNFEAPQYQVNGNETQTQTINTNTTFTFYYDEVFLVEVKA
                     RPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAPMFTNFVLDPRESSSNTVTLNNVT
                     SNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTVYEGDSFSTSWKNMTDQNYFLCRN
                     DDSSVTVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELAWLNSYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVD
                     EILSDTGAVNAMVMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANP
                     IFK"
     gene            complement(5108..5299)
                     /locus_tag="IV142_002502"
     CDS             complement(5108..5299)
                     /locus_tag="IV142_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486094.1"
                     /translation="MYADKEKIEWLLFETGKTAYEISKHSSVAESTIADLIKKKSAIE
                     KMRFDNAAKLTKYAESISS"
     gene            5487..5942
                     /locus_tag="IV142_002503"
     CDS             5487..5942
                     /locus_tag="IV142_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486095.1"
                     /translation="MKKLIMVFTSLIAMIVILGGCGSEKNKQDSQDNGVLKPGTVWKE
                     EVGGLVHNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGVDLFIG
                     KTALIVPYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            5939..>6033
                     /locus_tag="IV142_002504"
     CDS             5939..>6033
                     /locus_tag="IV142_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486096.1"
                     /translation="MIASLFIYPEGGIAVAKAKTPFEKAASKILER"
BASE COUNT         2001 a          991 c         1166 g         1875 t
ORIGIN      
        1 attatttctt cagaacaatt agacaaccag gggcatgaaa aacaagaagg agtaaatggc
       61 gcgaagtttt ctgtttatga tgtatcggac attttacaaa agatggatgt taaggatctc
      121 actactgacc aaattgaatc ccaattaaaa aatcgagtga aaaaactatc cagtgatcaa
      181 ttgaagctag tttctaatgg agaaacaaag acaattgatc aacaaacagg tgtctttgaa
      241 ttctcggtag aagtacaggc aaatcagaaa caagcttatt atattgtcaa tgaatcaagt
      301 cctgaaaaca tttctaatag cgaggatatt ttgctattaa cacctgtttc tgataaaaac
      361 ggtgaattct taaaggatgt gtggatctat cctaaatcag aagcttcgca acctaaagaa
      421 gaagtaaaaa aaatcgtttc tactggcgta aagaaaaact tctttgagaa ttgttgggat
      481 tttgttactc acttgttttc tagagattaa ggaagaaata aaatgaaact gatcaaatta
      541 ttattattca atttttcttg tttaaccgca gtctttttgt actgcggttt attattttct
      601 aaactatcta ttttgccaag ctaccaacta tacctaatga ggattttaaa cataccagct
      661 gcttccatgc cgctagatat tatggaaaag ctcattagga atccattatt tactacgatc
      721 aatttgatcg ttattgtcgg aatttcacta gtcatttata aaaaagaggg gatgaaggga
      781 tgaattttaa acactcgtgg acaagacggt taaaatatgt ttttcccata atgatgatgt
      841 taggtacatt gtttgggtta aaaacagcca atgtctttgc tgaaaaagtt attgtcgatc
      901 cgtcaaatcc aatccagaac gtaaagaata attttattat tccaaaagat gttcctaatg
      961 caaaaacaaa tattactgta aacggagcgt caaaagttta caaatatcat gttgatgcca
     1021 acaaaggtgg ttttacagac gactacgtag gattagtgga gggaaacaaa aatatacgct
     1081 atccttcttt ccaaaaagaa tacggtgaaa caaaccttgt tttagaaggc gtaacaacca
     1141 acaccaaagt caatattgac tatggaaaaa tcggtacgta taacgggaaa aaagtcaata
     1201 tcaagctagt tctatctaat attcacttat actcggatac gttgccgtgg aatctactag
     1261 ataacaacta tacaaaaact cattttcgag acgatggcta taaaaatacg aatggtgcaa
     1321 tgagtaaatc taaaaaaaga acagtacttt ggatctcaga caatcttttc tcaggaattg
     1381 tttaccattc aacacaaatg aatgtgcagc tagtagccac atatgaggac ggtagccccg
     1441 ttcaattttc aggagataca tttatcagtt ttaactcttt gaaccctgca ggaggaaaaa
     1501 gcacggatct taaaggagaa tatgcccact atgacaaaat gaatacgaca gattggtatg
     1561 tcagaaaaga tacggttctt tcagaattta aatctttcta caacaatctg aacgtggtag
     1621 gtggccaccc aggaggaagc tctaagctga cacaagcgga taatgatttc aataatttgc
     1681 atgataaatt aggagatccg aagtttggtc aaggtactgt gtcctttaaa atatctgaag
     1741 caaatccgac gtttgtgatt ggttcttcta atgttcaaac ctggtttact ttatcttctg
     1801 cgacaatctt tagtgttgtt cctgatcaac ctgaaaaaac aggggttgat aaaaatggaa
     1861 ataacgtgaa cgataaaatg ttgcaagtag gagacacgat tcaatatcga atcaaacaga
     1921 aggtgaatcg attaggagtg gatttactag cggtttacga tcgatttgag ttaatcgata
     1981 atttaccaaa agaagtaaat tacgtcgatg cacacgttga aagtggtaca aataaaaaat
     2041 tcgatgtatc tggtgaagtg acctatgaca aaacaaaaca ccaggtaaaa tatgcagcga
     2101 aagctgatac attaaaaaat cgaatgaagt ataacggcga aacttacgaa ttagtgatca
     2161 atgtaaaagt caatgaattg gccaatcaaa atagtgtagc caaaaatcag ggaacgtcga
     2221 tcattaataa agtagaaaag gatacaaata tcattaccgt ttatttccct aaaattccag
     2281 tgaaagaagt acaacaaaat ggaaaagatg taaacggacg aaatgacggt aagaagggta
     2341 cgccgacagc tcctttaaac gcagggtctg aagtacaata tctagtcaca caaaaatggc
     2401 acacaaaagg cgtagacgct gcgtcagatc actacaaaca atttagtatt caagatccaa
     2461 ttgaagctcg tttgacatat aaagaaggtt ctgcgcaagt aatagataag agtaaaggaa
     2521 aagatattac ttctgaaggc acactaacct atgatagcaa ttcaagaacg ctgaagtggg
     2581 aagcttccgc tgacttctta aataacaatc ttttagatgg acgagaaatt cagctgatat
     2641 ttacagctaa aactccatta caatcagaaa aaaatattga taaccaagcc gtagtagcag
     2701 tagataatgt ttctaataaa acaaatgttg tgacaattgg ggttgatcct aacttgccac
     2761 aagttattgt tcctaaaaca ggttctacac atttagtaac gattttagcg gtcagcttac
     2821 tattacttgt gttagcaact tttggctacg cagttctgaa gttcaattaa acaaaaaaac
     2881 aaggctgaaa atcagccttg tttttctatt tgaaaatggg attggcacta ataaatacgt
     2941 tgtctgaaga aagtgatccg tctgcagaaa cttcataagc atgggataaa ccaacgatac
     3001 tttgattgtt gtaaagcaag tttgcgttat gagcagcgct atctttccac ataaccatag
     3061 catttactgc tcctgtatct gaaaggatct cgtcaacgta aaggtagaaa ctattgatat
     3121 tttctccttt agcaccgtag cagcctaagc tttctaagag atcttggaaa gtaccgccgc
     3181 caggtcggta atgactgaag cttacttgta attcttttgc acgaatatct gcagcttgtt
     3241 gaacgatatc attgaattgc attggggcaa cgccattttg ttgacggtag ctatttaacc
     3301 aagcaagttc tttttgacgc acatagttgt ttaaatcact tagtgagata tttttgtatt
     3361 tgaaagtgat cgttcgattt tggtcaacat tattgattac acgttgtcca tttttatcaa
     3421 cggtaacaga tgaatcgtca tttctacaca agaagtagtt ttgatcggtc atatttttcc
     3481 aagaagtaga gaaactatct ccctcgtaaa ccgtttgtgt ttcagtagat aaaacagcgt
     3541 ttgtgtcaac gtttatatgg ttcacagtga cgttgtattg atgagtatag gcaaaattga
     3601 ttccttgatt acttgtaacg ttgtttaaag tcacagtgtt gctagagctt tctctaggat
     3661 caagaacaaa attagtgaac ataggtgctt caattgtgat gctttgtcca taaacgacgg
     3721 gataactctt actgtaatta tagagttctt ggatatttgg atcagtatcc gagactttat
     3781 ttggacgagc tttcacttca actagaaaga cttcatcata gtaaaaagta aatgtggtat
     3841 ttgtgttaat cgtttgtgtt tgtgtttcat tcccatttac ttgatactgt ggtgcttcaa
     3901 agttagtcaa gtcggttcta gcttttgcag tgtaacttgt accagctttt actgagacat
     3961 gttcttcttt tagcagcgtt ccagtttgtc gattcatatg acggacagtt accgcatatt
     4021 ctgaaggttt aggtttgtgt tgtaaccctt taaatgcaga atcaagacta tttttagcat
     4081 tatttacttg ggtttgagaa gctttagaat cgtctagtac ttttttagca ttggataaag
     4141 cagtctgaaa attattccaa gatccttctg taaaatctcc tttagcagtt gattttactt
     4201 ggttgtacaa ggcttgtaat tccgttttgt tcacagtagg agctggtttt ttctttaatc
     4261 ccttgtaagc agaatctaat gtgtttttgg cactatccac ttgtttttga gtcgcttttg
     4321 agtcatttaa cactttttta gcattgttta aagcggtttg gaatgcgttc caggtattgt
     4381 cggtatagtc acctttttta gttcccttaa cgctcgtata aagagcttct aaagctgatt
     4441 tattcacttt gggtttagag gtgtcagcac cataccaacc aataccttcg tatttccagc
     4501 catagtcttt ggttaaccat tgtacttctt tccaacttgc agtgtagtga tgcgaaccag
     4561 aggtaacacc tggaaggaaa aggcggtaga tcggtttatt atttttttcg gcagaacgcc
     4621 aggcaggccc ttcataagtc caaccgtaat ttttggtcaa ccatttcact tcatcccaac
     4681 tagctgtgta atggtgatca cgaaggcctt tattgtataa acgatagaca aggtggccac
     4741 cgctagtcgg tgcttcccaa gcggggcctt cataattacc ccacttaatt ttcactaggt
     4801 gatcacgttc ttttgtgcta gaggtgtaaa aatgttcatg gttatttgga ttataaaggc
     4861 gatacatgac gttagttgaa gcagcgtcag cagatccagc aagagcaact gtggcagcaa
     4921 atgttccaat agctataaca gtttttgggt ttaataattt catgttttca actccctatt
     4981 aaatgattaa atattttgtt tacattcata atataccacg gtataccgag gtagtcaacg
     5041 gtataccgtg gaaattttga aataaaaaag ccgccctatg aagagtgtaa ggatggctgt
     5101 ttatatgcta tgaagaaatg gattcggcgt atttggttag tttggcagca ttatcaaatc
     5161 gcattttttc aatcgcactt tttttcttta ttaaatccgc aattgttgat tctgcgactg
     5221 aagaatgttt gctaatttca tatgctgtct tgccagtttc aaatagtaac cattcgattt
     5281 tctctttatc agcgtacatt gcaatgctcc ctcttgattt tttctttatt atagctcttt
     5341 tttgtaataa atcacaatat caagaggaaa agtttctata gaaaggtggg aaattatgag
     5401 tatccgttcc ccgtgtatca aaatgaggtt tgttgctact ttcgttattt aatatactaa
     5461 aataaaacaa cgaaagaagg aatagaatga aaaagttaat aatggtattt acttcgttga
     5521 ttgccatgat tgttatttta ggtggatgtg gtagtgaaaa aaataagcaa gatagtcaag
     5581 ataatggcgt gttaaaacca ggaacagtgt ggaaagaaga agtaggcgga cttgttcata
     5641 atttaaaaat tatagatgat acaacatggg aatattccga aagtgtttgg catcctgaac
     5701 aagtacaaat aacagttgag aagctaaaag actataaagg attagaaaga tataaggtaa
     5761 tcgattcagg tggagtagat ttattcatag gtaaaacagc cttaatagtt ccctataaca
     5821 aagacggttt agaaacgttt aaatttattc caacaaacga aaaagaacat cgagataaaa
     5881 gtgaaatatt aaaagaatac agtactaaat ctggatacaa gttacaaaag actagcgagt
     5941 gattgctagt ctttttattt atcctgaagg aggaatcgca gtggcaaaag ctaaaacgcc
     6001 atttgaaaaa gccgcgtcta aaatcttaga acg
//
LOCUS       DACDEG010000119         6012 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.124, whole genome shotgun sequence.
ACCESSION   DACDEG010000119 DACDEG010000000
VERSION     DACDEG010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.124"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            453..1025
                     /locus_tag="IV142_002505"
     CDS             453..1025
                     /locus_tag="IV142_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP8486097.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDALDEDED"
     gene            1257..1874
                     /locus_tag="IV142_002506"
     CDS             1257..1874
                     /locus_tag="IV142_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006845390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8486098.1"
                     /translation="MNYIAEKAHTKQYQKGEFLFRSQEKEDTLYIINRGKIRIYRLAD
                     SGKEQLVRILNPGDFTGEWTLFNPDAVHEEYAEATRDTVVCMIQQKDVQDFLEQYPAI
                     SLKLLAEMSKRLESSERQTTQVAVESVITRLVLFLAENVEPEMGNSPTITLPMAKKDI
                     ASYLGTTPETISRKFGELEDEGLIQQLSGKRIKIQDLDDLLLYSE"
     gene            2102..2440
                     /locus_tag="IV142_002507"
     CDS             2102..2440
                     /locus_tag="IV142_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003102891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486099.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQIEWDSY"
     gene            2425..3279
                     /locus_tag="IV142_002508"
     CDS             2425..3279
                     /locus_tag="IV142_002508"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003125438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP8486100.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            3426..3659
                     /locus_tag="IV142_002509"
     CDS             3426..3659
                     /locus_tag="IV142_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HAP8486101.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            3857..5761
                     /locus_tag="IV142_002510"
     CDS             3857..5761
                     /locus_tag="IV142_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP8486102.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISVGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPTEKVKIS"
BASE COUNT         1945 a          995 c         1341 g         1731 t
ORIGIN      
        1 gtatacccta aatagacatc ataataacat tgaaaaacat ggttgtcatt tttctagata
       61 aatggcataa attttcagcg ctaagtgaca aaagcaacta atttttcatg agagattttt
      121 aaatatctca atctatctcc aaaattagtt gtttttgtag ctatagttga ctatgaatat
      181 gacaaataat tagaaaaaac tgtgccaatt aagtataaaa atgacatttt aggccctact
      241 ttaatgaaat ttttgtttgg agggaacgac ttcattcctt tgattatttc agttgaattt
      301 aatagagaag cctggcgtct attaagaaat aattttcttg atgagcgtca atttttttaa
      361 cggaaatcta tcctatacta aattcagtaa agaaaaagga ggaatcaaag aaagctattt
      421 taaacaaacc aaaattatag gaggaataaa tcatgactga aaataaagct actcaagaaa
      481 gaacacccca agaaagattg caagaagaac aagaacacaa ggaacatgtt catcatacga
      541 aaattaatgc agcggccatt gcggatcatc ttttaggcaa catgcataca ttacatgtga
      601 aactgcacca atatcactgg tatgtaaaag gagccaattt ctttacgttg catgaaaaat
      661 ttgaagagtt gtataacgaa aatgaaaaat ggttcgacaa acttgcggag cgtctaatta
      721 cttctgggca taaacctgct tcgacaacgg ttgaatttga aaaatattcg atgctttcgg
      781 aagatgcagt taataaatac gctaaagcag aagacatggt tgaaaatatt atcgaggact
      841 tcagaagcac tcgtgaccta actattcgcg cgattcgttt agctcaagac gagggtgacg
      901 atgctttaga agatacattg attgctttta aaaatgactt agacaagaac atttggatgc
      961 tgcaagcgtt cattggtaaa gaagcattag aagatgatga cgctcttgat gaggatgaag
     1021 attaaggtaa aagtagaagc tgatttaggg attggaattc tttccagtcc tttttccatt
     1081 ttcttgatgt gcatcaattt ttttgtagta agagacttat ataataaaag cagcgaatat
     1141 aaacaaaata aataaagggg gagaatgaat tgactaaaca tacccatcat gaaattggcg
     1201 accacgcagc ctgtatccgc ttggttccaa tattcaatca cttagaggat agcgcgatga
     1261 attacattgc tgaaaaagcc cacacgaaac agtatcaaaa aggagagttt ctgtttcgtt
     1321 cacaagaaaa agaggatact ttatatatta tcaatcgtgg gaaaatccgt atttatcgat
     1381 tggcagactc tggcaaagag cagctagtgc ggatcttgaa ccctggtgat ttcaccggag
     1441 aatggacctt gttcaacccg gatgcagtac acgaggaata tgccgaagca acccgagata
     1501 ccgttgtctg tatgattcag caaaaagatg tccaagattt tctggaacaa tacccagcta
     1561 tttctttaaa actattggcg gaaatgtcta aacggttaga aagttcagaa cgtcaaacga
     1621 cacaagtagc cgtggagagt gtcattaccc gtttagtttt gtttttggca gaaaatgtgg
     1681 aacctgaaat gggcaactct cccaccatca ccctgccgat ggcaaaaaag gacattgctt
     1741 cctatttagg aacgacacct gagaccatca gtcgcaaatt tggagaattg gaggacgaag
     1801 gattgattca acagctgtct gggaaaagga tcaagattca ggatttagat gatttattgc
     1861 tttacagcga ataatcggac acactttttt ctacacgttg gtgttctgtc gctcttcatt
     1921 aacaacggga cagaactcag tgcttttgct tccatctgtc cgtttttggc ttttttaaga
     1981 cagaacgacc ttcattcatt ggaaaaataa aagcacaaac atgataaccc tcaagttttt
     2041 ttgttttatt tttctttaag atagggttgt cgaacaaaac gtaaaaataa gtagggtgaa
     2101 tgtgcattgt ttttgggtga tagttgccat cacgttgtca gcagcggggg cattctttgt
     2161 cgtccctttc accaagaaca cgattggcgg cgaagcaaaa acgtctaacg ttgttgaggc
     2221 agataggtta gctatggaaa aagaagagga agttgtaaaa ggagccggcg taatcggaca
     2281 cggaaaagtg gcgtattacg aagcgagtga ccagggagat aaaagtgtat tcactgtttg
     2341 gtttccatgg agcttagccg gaatcttgtt cagcatcacc atgactagaa tgacgcgaaa
     2401 aattgaagaa atggagcaaa tagaatggga ttcgtattag gcgttttgaa tccccatgtg
     2461 gtggagattg gttctgttgt gattcattgg tatggagtca ttattgcagc gggcatatta
     2521 gcggctctcc agttgagcac caaggaagca aagaaaaaag gactggaaga ggataccatt
     2581 atcgatatgg ccttatgggc aattccgatt ggactcctcg gcgctcggct ttattatgtg
     2641 ttatttgaac ttggttatta tctgcaaaat ccaggacaaa tccttgcgat ttggaatggc
     2701 ggcattgcga tttatggggg attgattgca ggagggggta cgctatattg gtatgcaaag
     2761 aaaaaaggta cttctttggc actcgttctt gatattttag cgcccaatgt attgttagcc
     2821 cagtccattg gtcgttgggg caattttatg aatcaggaag ctcatggtgg gccggttact
     2881 cgtcaattct tggaaaatct ttatttacct gaatttatca ttgaacaaat gaatatgaat
     2941 ggtacctact accatccgac ttttttatac gagtcattgt ggagtttgtt gggctttgtc
     3001 ctcatcgtca ctatacggaa ccgaaaacag cttttgcgtc aaggagaagt cgctttgagt
     3061 tacgttttgt ggtactcagt gggtcggttc ttcattgaag gtatgcgaac ggacagttta
     3121 tggattggcg agtggatccg cgtttcgcaa gccttgtctc tggccctgtt tattggcgcg
     3181 attgtagtat ggtttatacg cagacaagat tatccaccaa tttcttatta ttctgatggc
     3241 aataaggggc agaaaaagac tattaagatg gtgaattgag caaaaagata aaaagaagtg
     3301 caaaacttga tatgcgtcaa ttttcgtttg ctaaaagtga gctatactac agttgtaaag
     3361 aggaaaaggc agtgttcatt aatctgctgc cgataaaata aaaacacata aaggagatag
     3421 gaattatgga aaaagctata ttgcaattag aaacgttgtc ttgtccaagt tgtatgcaaa
     3481 aaattgaagg tgccgtgaaa tcagttaacg gtattgataa agacagcatt aaagtattat
     3541 tcaactccag caaagtcaaa accaattttg attcagctgt cacttcgatt gaagagattc
     3601 aaaaagctat cgaaaacgta ggctatccgg ttcttaaagc aaaagtcaag gccgtttaac
     3661 agattaaatg aactaggcaa ttctggaaga aaagctattt ttccaggatt gctttttttg
     3721 ttgcaatccg attcgaggtg cttagaaacg ataaaagaga actttctaaa cttgatgtca
     3781 gtcaattaat tacttaggca attatcttat actgtgttta tcaataaaag agaaattaaa
     3841 tttggaggga aaaagcatgc aacaatatat attaagcaag aaaaatgtta tcaccgtcat
     3901 cagtggattg ttaatcgtac tcggtttctt cagtcacttt gttttagaaa acgtaggact
     3961 ttcagagtgg tctttgatca tcgcctctgt ctttgggatt acgccaattg ccattcaagc
     4021 gtttcaagca atgaaagtca aagtcatcag tattgatgtc ttagtcagta ttgcagcgat
     4081 tggtgcgctt tttattcaaa attatgaaga atcggctatt gtcacgttct tattcttatt
     4141 cggacactac ttggaacaac gaacattgaa ccaaacgcga tctgctatca aagaattgac
     4201 tgaaatggca cccgaaagcg ccttgaaaca aatggataat ggcgaatttg aagaagtaga
     4261 agtggatgat atagatgaag gggatatctt gctcgttaaa acgggtgcga aagttccagt
     4321 agatggcata gtccttacgg gtgaagggca tatcaatgaa gctagcatta caggtgaagc
     4381 tattccggtc aacaagctag ctgatgcaga agtttttgca ggaaccattt tagaaaacgg
     4441 aacgattcaa atcagagctg accgagttgg tgaggacacc acatttggaa aaattattga
     4501 actcgtggaa gaagcacaag attctaaatc cgaagcggaa cggttcattg atcgcttttc
     4561 taaatactac acaccagctg tcttggttct ggcaattgtt gtatgggcgt tcagtcaaaa
     4621 tattgagtta gcaatcacca tcttggttct aggttgccca ggcgcattag ttatcggagt
     4681 gcctgtctca aacgttgccg gtattgggaa tggtgctcgt aacggtgttc ttttaaaagg
     4741 gagtgaagtc attcaagact tcagtaatgt ggatacaatt gtatttgata agacaggtac
     4801 tttaactgtc ggaaacccaa ccgttgcagc gactgaactg tatgaaaaag atcctgctga
     4861 aacgcttggc tatctggcca gtgtggaacg agaatcagat catccattag caaaagcggt
     4921 cttaaatcaa attggagaaa caaactttta tcctgttgaa ggaactgaag ttgtgaaagg
     4981 cggcggaatc gtttcaagtg tagctggaca tagagtggct gttgggaatg tggccttgat
     5041 ggaaaaagaa aatgtcactc tcagcaaaaa agtgcaaaaa gatgtcaaac ggtttgaaca
     5101 acaagggaac tctctcgttt tgacagcggt cgacggtgaa ttaaaagtac tgatgggcat
     5161 tcgcgatcaa gtccgtccgg gtgtgaaagc taatttgcag gaattaaaag acttgggcgt
     5221 gaaaaatcta gtcgttcttt caggagataa ccaaggaacc gttgatgtgg tcgccggcga
     5281 acttggtttg accgaagctc acggctacat gttgccggaa gacaaatcgg cttatatcgg
     5341 aaaacttcaa caacgtggtc aaattgtagc ctttgttgga gatggcgtta acgatagtcc
     5401 gtccttagct ttagcagaca ttggaatcgc aatgggaagc ggaacggacg tagcgattga
     5461 aacatccgat gtcgttttaa tgaactcgaa cttcagcaac ttgcctcacg cattaggatt
     5521 agtaaaagcc accgcaaata atatgaaaca aaatatcgtg atttcagttg gagtagtgtt
     5581 ggtcttgttg accagcgtct tcttcagcga atggatgaat atgtctatcg gaatgttggt
     5641 tcatgaaggt agtatcttgg tggtaatttt caatggcatg agattaatga agtataagtt
     5701 gaaaggtcgg aaagaacaaa aagaagtatc agcacctaca gagaaagtaa aaatatctta
     5761 agaataaatt agaagaggct gagacaatag tccatccttg aaatgacaaa agcggaaact
     5821 cagattttta aatctgggat tttccgcttt tttgagttct tgaaaaaaaa taagagtata
     5881 gagtatgttc tttataaaat taattttcgt gtaaattatt aaaaatttaa taataaaatt
     5941 taaattccta ttaagttgta acacatgaaa ttgcttaaaa tagtgtatca aaaaccttgt
     6001 tttattaatg tt
//
LOCUS       DACDEG010000120         5957 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.125, whole genome shotgun sequence.
ACCESSION   DACDEG010000120 DACDEG010000000
VERSION     DACDEG010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.125"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            721..1455
                     /locus_tag="IV142_002511"
     CDS             721..1455
                     /locus_tag="IV142_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8486103.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1471..2640
                     /locus_tag="IV142_002512"
     CDS             1471..2640
                     /locus_tag="IV142_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8486104.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2656..3651
                     /gene="lacD"
                     /locus_tag="IV142_002513"
     CDS             2656..3651
                     /gene="lacD"
                     /locus_tag="IV142_002513"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP8486105.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3730..4656
                     /locus_tag="IV142_002514"
     CDS             3730..4656
                     /locus_tag="IV142_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP8486106.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4675..5925
                     /locus_tag="IV142_002515"
     CDS             4675..5925
                     /locus_tag="IV142_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8486107.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         1999 a          970 c         1218 g         1770 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaaa gtttgaaaaa tgctatttcg aggtagaagg atattatata
      121 agcacggttg actaaacgaa gtgataataa aaaaagttat tcaagaataa gaaaaattat
      181 gatacgaatt cagataaact ggagaataca gagaccttta aaagggataa gaaacaacat
      241 aaattcctct taagagagaa cgaaaggaca aagccagcaa cttgatacac gagctagtat
      301 tagaggaatt tattctcata gactcccttt tgaatacgtt tcaactttaa ttttcatgtt
      361 cataaatgat ttgaaaccaa agcaaatgcg ttgcactttt cctccgtctt ccgaaaatcg
      421 ttcaattcac tggtaagtag tcatttgata gtttgaggca aattgttgat tgatgaaggg
      481 taggagctaa aaaaagatac cagttgaaga gagaaaaaga aaaaaataga gggtgttctt
      541 gaatgaaaaa agttggaaaa ttatttttca tttcctttta ttcctattaa agtcagtgat
      601 gtcaatcata ttaacaagac taaaaatgaa aataaaaaaa agattgttct tagtattgac
      661 tcataaaaaa taccactact atacttacat tgtggttata gccagttagg agagacatcg
      721 atgcaaaata cctttcaatc aaaatcactt tattatcagc tggtagaaat attgaaacaa
      781 cgaatcgaaa acagtatggt cgctcatgac aagctgccat cagaaagaga gttgaccagt
      841 ttttatggcg ttagtcgcac gacggtgcga ctagcattgc aagaactgga aaaaatgggc
      901 tacatttatc gacaacatgg aaaaggaacc tttgtttcgg aattaaggac aaatgcgact
      961 gatctagctg gcgcatatag ttttaccgaa caaatgcggg caatgggcaa acatcctgaa
     1021 accaagatat tggccttcaa gaagattgaa gcaactaaat atttagcgag tcatctgaat
     1081 gttcaactag gtgagccttt atttcaatta gaccgattgc gcttagctga tgatttgcca
     1141 atgatgatcg agaaatccta tttgccagtg aagctattta tgtccctttc tcaagaaatt
     1201 ctatttctga aacctttata taatttgttt tcagagaatt tcaatcaaat cgtacggttg
     1261 gctgacgagg aattttatgc cagcattgtc cagaaagaag atagtgagtt gctaaatatt
     1321 ccagaaggat caccagtgct ccatttagtt cgctcaacct ataatctcga gaacgaagtg
     1381 attgaattta cattaagtgt agcgcgggct gataaatttc attataagat caggcatctg
     1441 cgttccacta actgaattgg aggaaagaaa atgtttatgt tctcaaaaga agaactacga
     1501 aagctaggag cagaaaatac tacccgagaa attcgacaac aaccggaact gtggcatgag
     1561 acgtttgacc gttatcaaga aacagttgca gatttagatc gtttgtttac ggatgcgaaa
     1621 caacatgctg gagagcgacc aatccgagtc atttttacag gggccggaac ctcgcaatat
     1681 gtgggagata cactaattcc gtttcttact agtcaaggaa atacgaacca gtttattttt
     1741 caaagtattg gttcgaccga tatcgttgct acgccaaaag aatacctgat tcctgatgag
     1801 acaacgatct tggtctcatt tgcccgtagt ggaaatagtc cagaaagtct agccgcagtt
     1861 aagatggcca atcagctggt caacaagatc tatcatatcg tgatcacctg tgcaccagac
     1921 ggattattgg ctacacaaat gcctacaaaa aaagaaaatc tagttttact aatgccaaac
     1981 aaagccaatg acgctggttt tgctatgaca agcagtttca gctgtatgac gctgactgcg
     2041 ctacttattt ttgatcaagc aagcaatcag caaaaagaaa gatatgttca tcaattaagc
     2101 cgactaggag aagaagtcat ccaacgagaa tcagagatcc aagggtatct ggcatcagac
     2161 tttcatcgca tcatttactt aggttcggga agcttaggcg gattgactag agaagcgcaa
     2221 ttaaaagttc tcgagttaac tgctggaaaa atcgcaaccg tatttgatac atctttagga
     2281 ttcagacatg gtcccaaatc ttttgtcaat gagcagacat tggttttcct gtttgtcagc
     2341 aatgactcat atacgcagaa atacgatgta gacatgctgg aagaaatcaa aaatgacggt
     2401 attgcccaac gcgttgttgg aattggtcaa aagggttcat tgtttagtgg tgattccttt
     2461 gaattagttg cagattcgtt actgccggat ggctatcttg ctttagcaga tatcatggta
     2521 gcccaaacga tcgcattact ttgttcggtg aaggttggta atacaccaga tacaccatcg
     2581 ccaagtggaa cagtcaatcg tgtggtcaaa ggcgtaacga tttatccata tgaaaaatag
     2641 gaaagagtgt gaagcatgtt agaatggtcg gaaaataaaa gacaagcaat ggaacgtcta
     2701 tcgacgccag aaggcattat taccgctttg gcgatcgatc agcgaggcgc gctgaagaaa
     2761 atgatgtcgg ctttagggat ggaagcgaca aaggaaaaaa ttgagcagtt taaaacacta
     2821 atttcccaag aattgactcc ttatgcctca gcaattttat tggatcctga atacggactg
     2881 cctgctgcac agaagcgtgc agcagcgagc ggtctgctgt tggcatatga aaaaactggt
     2941 tatgatcaaa ccgtacccgg ccgcttgccg gatctgctgt cggactgttc agttctgcga
     3001 ttgaaagagt taggtgcgga tgcggtgaaa tttttactgt attacgatta tgaagaagca
     3061 gaggaaatca atcggcaaaa gcaagttttt gttgaacgaa ttggaagcga atgcatggct
     3121 gaaggactgc cattttattt agagttattg tcatatgatg ctaaaaatgt agatacgaaa
     3181 tcgctttcct atgccaaagc aaaaccgcgc aaagtgatcg aaatgatgaa agaattctcg
     3241 aaaacccagt ataaagtaga tgtcttaaag gttgaagtac cagtaaacat gtcatttgtt
     3301 gaaggaaatg caacagggga gatcgcctat acacgtcaag aagctgccgc ttattttaaa
     3361 gaacaaagcg atgcaacaga tttgccgtat atcttcttaa gtgctggggt atcagcggca
     3421 ttattcatgg agacattgcg ttttgccaaa catgctgggg cacgattcaa cggtgtactc
     3481 tgtggcagag caacttggaa agatgcagtc gctcctttcg ttgaaaaagg agaagcggca
     3541 actttagaat ggctgacaga acaagggaca caaaacattc gtcaattgaa tgaagtgata
     3601 agagaaactg ccatcccatg gtatgagaaa gtaagtgaaa gcacttgtta atgtttctaa
     3661 tatattagta gtgataagag atatgtgtga ttataattta tcattattga aactgggagg
     3721 atattttata tgaataaaag attgttagat tattcagctt cagatttttt gaaagctaag
     3781 ccgatggaat taaagcaggc aattttggct tctgaaggta gaacaattat ggcagaaaat
     3841 gtgccaagta gctgtcctta catgggaaaa tcagtgacaa atgcagaatt ggagagagct
     3901 gctggagctg atttaatttt gtttaatgca ctagatcttt ttaatcctca aatttcaggt
     3961 gtacctttaa ctctaaaaga gaatccaata atctggatta aaaaagcttt aggaagagct
     4021 attggtgtaa atttagaacc tgtagacaaa gaagttttga tgagtgaaaa tcgaactgaa
     4081 atttcaagtg gtagaatcgt ttctcctgag actttaaaaa aggcaaatga attaggcttt
     4141 aattttattt gtttaactgg aaatccagga acaggagtat ctaatagaac tattgagaaa
     4201 gcaataacat tatccaaatc aatttttaat ggattaatta tcgctggaaa aatgcatggt
     4261 gctggtgttg atgaaccaat tatgaactta gatatagcaa aaagatttat taaaacaggt
     4321 gtagatattt tattagttcc tgctccatac actgttccgt atttcatgga aagtgatttg
     4381 cgaggaatcg ttgattatat aaaagatttt aatagaaata aatctattga ggaaaaagtt
     4441 ctagtactaa cggctaatgg aacaagccaa gactcttctg accaagaaac tattaaaaaa
     4501 attgcattag catcaaaagc atgtggagct gatattcaac acatagggga ttctttaaat
     4561 ggtatttgtc ttccagaaaa tatttttgct ttgggtcaag caattcgggg atatcgtcat
     4621 caaattatta tgttaggaaa atctaattat cgttaattaa ggaggaaatg aaagatgatc
     4681 gacaaagttc aaaaaatagc tgcaaaaatt agtaatcaaa gacatctact agctttaaga
     4741 gacagcatgg ctgcagttat gccattaatc attattggtt ccttttttat gttattagca
     4801 aatttcccga tagaaggatt tacaaaatgg ttatctgaaa ttggagtaca atcctttttg
     4861 aacaaagcat cagattcaac atttggtatt attggattag ctgttaccta ctctttggga
     4921 cacaatctag caaagactta tggtgtagac tcattatcag caggattatt atctttgagt
     4981 ggatatgttt tacttacacc tttattggat gcagatggtt ctataggatt tccaacaaaa
     5041 tactttggta cctcgggtct ttttgttggt attattgtag cacttgtatc tacagaaatt
     5101 ttccggtggt ttgtacttaa aaatattaca attaaaatgc cggcaagcgt tccaccaaat
     5161 gtaagccgag ctttcgttgc tattattcct ggcttttttg tcgttctact atggtttatt
     5221 gttgttgtaa tttgttataa acttggaatt gaaaatgttc atgctcttat tgcagataca
     5281 ctcgcaaaac cactatcttt actgacgaaa actttaccag gaattatatt ggttatactt
     5341 attcaatgtt tcttttggat gtttggtatt catggtgcac aggtaacggg gccaataatt
     5401 gaaccgttat tattgcaaaa ctctgatgta aatagaattg cttatcaagc tggaaaagaa
     5461 cttcctaaca ttataactta tgaattctta tataactttg ttttttctgg tggtgcaggt
     5521 tgtttatttg cacttgccat tttaacattt ttcttttcaa agagtcagga aaataaaact
     5581 ttaggtaagt tatcaatcgc tccagtaagt tttcaagtag cagagcccct tctatttggg
     5641 tttccaacta tattaaatat aaaaatgtta attccatttg taactgcacc agtagtaaca
     5701 acattaatta cttatttttc aatgagtatg ggattagttg caaaacctgt aggagcaaca
     5761 atcccatgga caactccacc gataattgcc ggatttttag cttctggtgg acgaataagt
     5821 ggtgcagtga ttcaagtaat aacaattgca ataaatgttt tgatatacta tccattcttt
     5881 aaattagatg ataatgccaa gctaaaaagc gaaaaaaatg attagaacta cctatatgtt
     5941 tctaactgta gttaagt
//
LOCUS       DACDEG010000121         5925 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.126, whole genome shotgun sequence.
ACCESSION   DACDEG010000121 DACDEG010000000
VERSION     DACDEG010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.126"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(479..1186)
                     /locus_tag="IV142_002516"
     CDS             complement(479..1186)
                     /locus_tag="IV142_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486108.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(1251..2087)
                     /locus_tag="IV142_002517"
     CDS             complement(1251..2087)
                     /locus_tag="IV142_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8486109.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2105..3514)
                     /locus_tag="IV142_002518"
     CDS             complement(2105..3514)
                     /locus_tag="IV142_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8486110.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3528..5351)
                     /locus_tag="IV142_002519"
     CDS             complement(3528..5351)
                     /locus_tag="IV142_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8486111.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
BASE COUNT         1827 a         1277 c          963 g         1858 t
ORIGIN      
        1 aataaactac gcgaaaggag tctaacgttg tttgatataa cttagactga tcgttaactt
       61 attttttata tttctacata gttatcttta gaaccatttc agatacatat gaaacaatta
      121 catatgattt tttcataaaa aaacagtact gtctagtagt ataaaatata caaatttctt
      181 aaaaattttt tcactaaaat cactacctga aacagtctaa aacacttata tatcaacgtt
      241 taatgaaact atctccgttt catttcagat acaaataaat atagtcgggc tccaaatccg
      301 aaaaaaaata cccccagtag cttatcctaa gctactggag atgtataagt acggtcatat
      361 aacaacgggg tagggtaaat aaatgaaaaa gatggaagaa tcattattca ataacttttt
      421 taaatacttc gttgtaatca gttacctgtc cgttcttaaa taccacagaa taatcttttt
      481 acccattttc aagtaaccga agtggaatgt ctttaccaac ttgctctgtc actgcattac
      541 tgatacctgt tagattagct aataaatctg ataaagattg agcatctgta gaaatctctg
      601 tatcagtatt tatcgtgatt cctttatctt catttacaag cttccaagta ggtggtatag
      661 ctgtgtatgt ttcgatgaat gaatcaattc tttcatctat cgtcttccca gtatctactt
      721 ctgtctcatt actcttagat gaatcgattc tttcatccat cgtctgctcc gtaactgctt
      781 ctgtctcatt attctcaccg gaaactctct tcacacccaa acttatatat tgtccatctt
      841 tagtgtgcat agttaaaatt cccacgcttt tactaataaa ttcactataa ccagttcttt
      901 caatagagac ttcaatgtat ttcacactat catactcaaa agaaaaatag acatgtaaaa
      961 gcattttgtt gtttacatct acataataat cctttgactc tacttgagac cttcctatac
     1021 ggaatacagc tttactgtct gatatctcta taccagcatc cttattatta atctgccaat
     1081 ctccaagtag ctcttctggc aagctagtag tttcttgtga acactttacc acttcactgg
     1141 tagaggtcga aacattcacc tccgcactgt tagaagaatt ttccaaggaa tcaggagcct
     1201 tagcacaacc aacaagtctt tatatattgt gcagccgata ttttcttcat tcagtacacc
     1261 cttgaaatat gcagtgttag ataaagcagt tcgtcctgat ttgctgaggc acccatctct
     1321 tcttgaacgt actccttgat tttcaaggcc gcccgatact ctttcggaaa acgctgcttc
     1381 actgaagcca gcaaaaaatc atccttctcc ggcaactctt ccttcttaat cattcgggca
     1441 ataaaaaact gtaaatgcgt cacaaatctt aagtaaagca tcgaatcttt atcaaccttt
     1501 ttttcgggaa acagatatcc tagcatagcc aagatatcct ttagtgctct taccgagagc
     1561 atggtttctt ccatatttgc ttgttcactt tgggcattta caatgtgaaa cgcaatgttt
     1621 ccagcctcct cttctggcaa ctgaacattc atttttttgt tgatcaattg gatagcatag
     1681 cagccgatct caaattcttt tgggtaatac ttttgaattt caatcagcag gcgattttga
     1741 aaaacaatgc ctttttgact gcgttcaatg gcatgcttca agtgatcaac caaggtaaaa
     1801 aatagacttt tattcagctt aaagtcaaac ttggcgttgg cataatcgat aattcgcttg
     1861 acgcaaataa ttagatcatc cggagcagcc tcaaacagtt taatgtaatc cttttttaga
     1921 gcatcagaaa gtgaatctaa tttaaatact ttttggattt tgtcagccgg aaccaaatcg
     1981 cctttcttta gttgaaatcc aatccctttc cccatcaaaa ttacttcttt gccttgctta
     2041 tctaagcaaa gaattaaact gttgtttaac gttttaatta ctctcattag attcaccgtt
     2101 ctctctaagc taagccattt cttgcgatta ctttttgata ccaaaagaag ctttcttttt
     2161 ttattcgttt taattcttta agattctcat cggttcggtc aacatatata aaaccataac
     2221 gcttattgaa gccttgatgg gtactaacaa catcaatcgc tgaccaaggt aaataaccaa
     2281 aaacatctac accatctgtt aaagctgctt gaatagccag aatatgtttg gctagataat
     2341 caattcgatc ctggtcatta attcgtctat cttcctcaac tatgtcgggt gccccatagc
     2401 cattttcagt aattaaaata ggtaaatcat agcgatcgta caatttcctc aatgttaatc
     2461 gtagtccagt agggtcaact acccagccat atggcgtttt atctacataa ggattttcag
     2521 ctgcccggta gacaccttct tctcccaaca tgatctgttg atcccccgct ctagcggaaa
     2581 catctgatcc gtcatttttg ctggcagcga tggtggcagt tgaataataa ttaatcgcaa
     2641 taaaatcggg tttaccggag gataaaattg cctgatcctc tgcctcaatt ttaggatata
     2701 atccacgatc attcaaatag ttttcaaaca aataattgta ttttccccga actgctacat
     2761 ctaagaaaga ccagcctctc aatgtttccc aattatgggc tgcaattgca tcttctggac
     2821 tggaggtagc ttgatacata gccgttaaat tgattgctgg accgattttt cctttaagat
     2881 tcaactgatg atacaagtga attactctag cttgagctaa taaaacatgg tgattaattt
     2941 gaaagagttc tttttttgaa ggcagctctc cacttggtgg caaaccaatc gctccaggat
     3001 gaaggatcat cgtgttttgc tcattaattg tcagccaata gttcaccttg tcaccatact
     3061 cagtaagcaa tacctgtaca tagtccacaa aataatcgat agttgctcga tttaaccaac
     3121 cgccctgctc ctctagaaaa taaggtaaat cgaaatggta catggtgaca atcggttcga
     3181 ttccctgatt caggcattca tcaattactt gatgataaaa agaaattccc ttttgattgg
     3241 ttttattatt ttcaatatcc ataattcgcg tccaagcgat ggaaaagcga aaagctttta
     3301 agcctaattc tttaaacagc tgtatatctt ctttatagtg atggtaaaaa tccgaggcaa
     3361 cttcaaaatt agctacccct tctgtatgct gatacttgtc aataatagac ggttgtttcc
     3421 catcctcctg ataggcaccc tctgcctggt aggcactaat ggaggcaccc cataaaaact
     3481 tctctggaaa tgctgaaagc tgtttgtatt gcacaaaaat cctctcctta actaatttgc
     3541 atcaatggtt cgtgttcctt taagcttccg cttgcttcac taattcctgc gtaattttca
     3601 ctgttggtca ctaccacaat cactgtatca tcgtagccag cgtttctaat ggcagcgaaa
     3661 tcggcgttga ttaataattg tcctgctgac acctgatctc ctgcttttac aaatacttca
     3721 aagggttccc cttttaaatt cacggtgtcg atccccacat ggatcaataa ttcaatacct
     3781 tctttagaac gtagaccaat cgcatgttta gattctaaaa tagtaatgac ttctccctca
     3841 acaggtgaat aaatgctgtt ttcttctgga tgaatggcta cgccctttcc caatagtccc
     3901 ttagaaaaga cagcatcgtt cacttcagaa ataggacaca cggttccttt taccggattt
     3961 tcaacaatga ctgcttcggc tattgccgtt tgctctgatt catcagaagt atcgtcaaat
     4021 ccaattagat aagcaatgat aaaggttgca acaaaggcga ttacacacgt aataattgcg
     4081 tgaacaatat tcattggatt ttctccaata aaggctggta aggctgctaa gccaggggaa
     4141 acaaaggcat atctgactaa accgctcaag ccagcataca aaccagcaat tgcaccacca
     4201 atcatggaag caactaatgg ttttttcagc ttcaaggtta ctccatagag tgaaggctca
     4261 gtaattccca ttaaagcagt gatcccagtg gacacagcta gctgcttcaa ctctttgttt
     4321 ttagttttcg cggctacagc aaaggtagca cctgattgag caatattaga agccagcatt
     4381 cctggcccat tgatcatttc agaacccgta ctgctcaatt gcatagtagc gattggcgtc
     4441 attgcccaag ctgtacccgt aaccaccaaa aatggctgca aaccacccat caaaaatgga
     4501 attaaccaag ctgctttgcc gttaatccat tcagccccca ttgctataag atcgttcagc
     4561 aacgttccaa acggtccaat gacaaccaac gctaaaggcc ccataattaa aaataccagc
     4621 atcggcttag taaagaattt aataaacgaa ggcgaaattc gatccgcaaa gggctctaca
     4681 taggacatga tccaaacagt aaagataatc ggtaaaactg atcccgcata atctgcagct
     4741 aataccggaa ttcctagaaa agaaacggca tcgccagtag aaaaaagctg tgaaatattt
     4801 ggatgaagca aaatgccagc aacagtaatc gccaaaattg ggttgacatc aaactttatg
     4861 gctgccccat aggctaatag tactggcata aaataaaagg ctgcatcagc aatagtattc
     4921 aaaatgatgt aagtttgact actgtctgat agtacaccag tcaaagtcaa gacggccaaa
     4981 atggctttta ccatcccggc acctgttata gctggaatca tcggtgtaaa ggtggtagaa
     5041 atgacactaa tcaatcttga aataatactt tcttgattgt ctgaagaggg gcccttttta
     5101 attgaagttc ccacttcctt ttgaatttcc ttgaaggctg tttggacttc attgccaatc
     5161 accacttggt attgtccacc gctatccacc acactaatta caccaagtgt tttttctaag
     5221 gctttcgtat cggctttcct tttatctttg agttcaaaac gcagtctggt ggcacaatgg
     5281 gttaagctga ccacattttc ttttccccca acctgttgca atacatttct tcctacttct
     5341 ctgtaatcca ttattctctc tccttaaagc agtaaaaaag accaagtcaa accacgcccc
     5401 aatcaacatt gaagtgttag tttgtcttgg tcttgcctgc ataatcagta acatgccaat
     5461 aacgttattt tgctgtaatc caataatagc acattatgat agcgcaatca acagggaatt
     5521 taatttttta tcttttcgtt tgaccgtata gggtaatata acagttagat cacatcaact
     5581 agggtatatc tgcgtatact tactgctaaa ataagtatat ctaatattaa ctataccagt
     5641 aaaaccattc ctacataacc agcgggaaat tcccaaaatc gtgaaagcat caaaaatgca
     5701 tataacggat cacacaactt caacaattct gctcttggga aaaattgatg aatttggtga
     5761 gtaaaggatg gaataaacag cattccaaca atataggcaa caatagtgat acttgttttt
     5821 ttggtaaaaa tcagtaagca atagccaatt tcaatcattc cgagatataa aggtatcaat
     5881 agaatgattt gggctagtat tcttaaaaat taactagcaa atgtt
//
LOCUS       DACDEG010000122         5697 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.127, whole genome shotgun sequence.
ACCESSION   DACDEG010000122 DACDEG010000000
VERSION     DACDEG010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.127"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            209..1483
                     /locus_tag="IV142_002520"
     CDS             209..1483
                     /locus_tag="IV142_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8486112.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
     gene            1537..2964
                     /locus_tag="IV142_002521"
     CDS             1537..2964
                     /locus_tag="IV142_002521"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP8486113.1"
                     /translation="MTKRAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDHLYMKPVRELEQLRVG
                     SGVETKVNLAGEIIIAQDASFKEALIDVLLTKSKNQEIKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            2977..3849
                     /locus_tag="IV142_002522"
     CDS             2977..3849
                     /locus_tag="IV142_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8486114.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNELLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            3944..4939
                     /locus_tag="IV142_002523"
     CDS             3944..4939
                     /locus_tag="IV142_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8486115.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKKKDN"
     gene            complement(5544..5651)
                     /locus_tag="IV142_002524"
                     /pseudo
     CDS             complement(5544..5651)
                     /locus_tag="IV142_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302409.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1964 a          783 c         1094 g         1856 t
ORIGIN      
        1 atttataaaa aatagtgcca ttaaaaaaga aaagtgacac accaataaaa gtcatatcct
       61 gtgacattat atggcattat ccaaatagaa taaccggttg acatgtaagc ggattccttt
      121 tataatttta ggagtataag tccttttcta aaaatattaa ccggttatca tgaaagggga
      181 tacaaatata tcgttggagg ataaaaatat gtcaaaggtt aacgtattta aaaatcaatc
      241 ctatttacaa agttcagcta cattattact attttttgct tcttggggtg tttggtggtc
      301 attttttcaa ctttggctaa catctgaatc aaatggttta gggttatctg gcagtgctgt
      361 aggaacagta ttctcggcaa attcgttagt taccttaatt ttgatgttta tttatggaac
      421 attacaagat aaattgtata ttaaacgaaa tttattaatt tttgcttctg tattagcgac
      481 acttgttgga ccatttttta tatggatata cgggccattg ctagataaca attttaattt
      541 aggcattatt atgggagcac tatttttgtc agctggatat ttagcttctg taggagtttt
      601 tgaagctgtg tcagaaaggt ttagtcgttt atttggcttt gaatatggac aagcaagggc
      661 gtggggatca tttggttatg ccttggtagc gcttttggca ggatttttat ttgtaaaaaa
      721 tcctcattta aacttttggg cgggatcttt ctttggttct ttactattgt taaatttatt
      781 attttggaac cctaaagttg aacgggaagc aaatcaaaat tttaatcaag aacaagctga
      841 atcaaatagt attccttctt taaaagaaat gtttgatcta atgaaactgc ctcaattatg
      901 gacgataatc atctttattg tttttacatg gacattttat acggtattcg atcaacaaat
      961 gtttccggga ttttatactg gtttgttttc aacatcagct aatggtgaaa aaatatatgg
     1021 gacattgaat gctattcaag tattttgtga agcgttaatg atgggaattg ttccaatcat
     1081 tatgagaaaa ttaggggttc gaaatacttt gttattaggt gtaaccatta tgtgtgtacg
     1141 aattggattg tgcgggtttg cctcgacacc attatctgtt tcatgcataa aaatgttgca
     1201 tgctttagaa gtaccattat ttacattacc aatgtttcgc tattttacac ttcattttga
     1261 tacaaagcta tcagcaaccc tctatatgat aggatttcag atagctgctc aaattgggca
     1321 agtgatttta tcaacaccat tgggaatatt aagagacaac gttggctatc aaccaacatt
     1381 taaaattatt tctcttattg tattactagc aggcatatat gcattcttta ttcttaaaca
     1441 agatgataga gatgttcaag gggatccatt tattcgagga taatggaaac caacagaata
     1501 aaaaacaatt ttatctagaa agtgagcgaa tatattatga caaaaagagc tatagaatta
     1561 acaaatacac gttatcgttt gggatatcat gtgtctgcac catcaggatg gattaatgat
     1621 ccaaatggat tttgctattt tgatgggtat tatcatgtgt tttatcaaca tcatccatat
     1681 agtgctgaat ggggaccaat gcactgggca catgctagaa gtaaggattt agttcattgg
     1741 gaatctttac cacttgcttt gactcctgga gatcaagaag atgaaggtgg ttgtttttca
     1801 ggaagcgcta ttgaaaaaaa tggtgtgttg tatctgttct atacaggaca tcactattat
     1861 ggagacaatg atccagatca tttttggcaa aatcaaaaca tggcgtatag tacggatggc
     1921 attcacttta ctaaatatga aaaaaatcct gtaatagcaa aagcaccaga agataacaca
     1981 catcatttca gagatccaaa agtatggaaa cacgatgaaa aatattacat ggttttgggt
     2041 agtcaagaaa aagatggatt aggtcgtgct attgtttatc agtcgttaga tttattaaat
     2101 tgggattaca ttggaccaat ttctaaagcg aaggaagtac ttacagaagg atttatgtgg
     2161 gaatgcccag acttttttga attaaatgga aaatctattc ttttattatc ccctcaggga
     2221 attgaagcaa atggaaaaga ttatttaaat ctatatgaaa caggctactt tatcggtgat
     2281 tatgattatg agacagcaaa attcattaga ggaaattttt atgaattaga taagggacac
     2341 gatttctatg caacacaaac aactttatct ccagatggta gacgtattgt tatggcttgg
     2401 atggatatgt gggaaagccc tatgcctgaa aaagtagacg gatgggcagg agcattaacg
     2461 cttcctcgag aattagagtt aattggagat catttgtata tgaaacctgt gagagaatta
     2521 gaacaacttc gagttggcag cggcgttgaa acaaaagtta atttggcagg agaaataata
     2581 atcgctcaag atgcttcatt caaagaagct ctaattgatg tactattgac taaatcaaaa
     2641 aatcaagaaa tcaaattttc attaaaaaca aaagatgaag agttaattaa tattttttat
     2701 tctaaggata aaaatgaatg taccttaact cgtaacggca aaaatgatcc aaggtatgga
     2761 accattcaga tgtgtgatag attatcactt cgagtgttta tcgataaaag ttcggcagag
     2821 atttttatta atgatggcga gcttgttttt acagagcgtt attatacaga aaatcaacca
     2881 agtatgcatt tgagtgttag tgaagaagct tttgtctcga gtacaattta tcatctaaaa
     2941 aaagatgcaa tttcttacga gtaaggaggt tttatgatgt taattggtag catagaagca
     3001 ggtgggacta aatttgtatg tgccgtggga gacgaggatt accgagtaca agaccaaata
     3061 atttgtccaa cgaccacccc cagggaaaca ttgaaaaaaa ctgttgacta ttttaaacag
     3121 tttgatataa atgcattagg tattgcatca tttggtccaa tagaaattcg aaaaaattca
     3181 ccaaaatcag gatatattac gtctacacca aaggtgggat ggcgaaatgt taatgtgtta
     3241 ggttatttac aagatgagct gaatgttcca atgtcttgga caacggatgt gaacggatca
     3301 gcttatggag agtacatcat gtccacttta tcgaatgaaa aaataaattc attagtttat
     3361 tacaccgttg gtactggcgt tggtggtgga gctgttgttg acggaaaact attaggatcg
     3421 tttggtcatt cagaaatggg ccatacgttt gtaaaacgcc atcctgatga tattgatttt
     3481 aaaggaattt gtccttatca tggtgattgt ttagaaggct tagttgcggg accaacgttt
     3541 gaagcacgtt ttggaaaaaa gggtgaagac gtttctttaa cggatcctat ttgggatgta
     3601 atggcctatt atctagcaca agctgtgatt caaacaacat tgattatacg accggataaa
     3661 atagtatttg gtggaagcgt agtaaatgaa aaattgttag aaaaaattcg tgttaatgtt
     3721 aatgaattat tggatgatta cgtggaaata ccaccgttaa gtaaatatat tacgcgtcct
     3781 ttaattaaaa ataatggatc ggcaacacta ggaaactttg ctttgggctt gaatcaatta
     3841 caagaataaa acatagaaaa aatatcgatt atcaggtata gtagttctat agagttcttt
     3901 ttttcgatag ttaattattg cattatttag gagagacaag agaatgcgcc caaaattaga
     3961 agatgttgcc aaaagagcaa atgtatcaaa aacaactgtt tcacgtgtat taaacaatcg
     4021 tggctattta agtcaaaaaa cgattgataa tgtttataag gctatagaag aactcaatta
     4081 tcaaccgaac gttgtagcgc gtcaattatt tcaaaagaaa actaacattg tcgggttact
     4141 atttcctaca gtagccaatc ctttttttag tgaactcgtt gaggcattgg aaaaaaagtt
     4201 atatgaaatt ggttataagg tattaatagg caattcgatg aataataaag aaaaggaaac
     4261 aaattattta aatcagttat tgtctgatca agtagatggt ttaattgttg gtacacataa
     4321 ccaagggatt caggaatata aatatcagaa tttaccgatt gttgcaattg atcgagtgat
     4381 gaatgaagat attccagttg ttgaatccga taactacaat ggtggaaaac ttgcaaccaa
     4441 gttattaatt gcacaaggtg caaaaaatat tattcataca aacggtccaa ttgacttaca
     4501 gactccggct aatcgtcgga gactagccta tgaagatact atgaaagcat atcagttaat
     4561 tccacgaact gtgacattag atttcaatat ttcttatgta aagaaaaaac agattttttt
     4621 ccaaatgttt gaggattatc ctaaaataga tggtattttt gcttcaaatg acatcgatgc
     4681 ggccctaata ttacaagtag caaaagaaaa ggggttaaat gtaccagctg atttattagt
     4741 agtcggatat gatggtactt taatgactcg gagtatatta ccagatctga caacagtaat
     4801 acaaccaatt aatgatatag cagacacagc tgttgctatt ttgatgaaac gaataaataa
     4861 agaagaaaca aaaaaggaat atattttacc tgtcacatta tggatgggga agacatcagt
     4921 aaagaaaaag gacaattaaa tttataaaga ctatcaatga taatttcatt attaattcgt
     4981 gatataaaaa gttgttaaat ctcttataat agactatgat acaaaatcat gcttttaatc
     5041 tgtgtagatg ttgccaaata tttgaaagac atagaacttg tcagaaaaac cttaacaatt
     5101 cctcaatggg cggataagct aggcaaaagg gctggcataa acttttcagt ccttctaaca
     5161 gaatcaattg cagataaagc agataacatt cttctttctg gaagaaataa ttagaactca
     5221 aaagggtcag aaacttaaaa gtttttgatc ctttattttc aagtctgatt tgacagattt
     5281 tcggcagctc atttatttat ggtcagaaat agttgctgaa attttaatct attagcaaat
     5341 agtccagaac gtatgaagag gattcggata tcatttgtca ttaacaccat aaatatttat
     5401 aaaattcgtg ttcacctaca attaaattta atatataata ttcgttgtaa gggcttataa
     5461 ttttttgtta atgaacaaga tatgtaattg atagtgtcaa tagctattca aaaatgtagg
     5521 cttttggccc ataaataaca aggttactta tatgataact ttttttcttt ataatcttta
     5581 atccgataga gctttccagt agtttgaata atcttagcgt ggtgcactaa tcgattgatt
     5641 agtgcatttg tgttcagtcc attgagacat aatagtggat agttttactg agaaaat
//
LOCUS       DACDEG010000123         5471 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.128, whole genome shotgun sequence.
ACCESSION   DACDEG010000123 DACDEG010000000
VERSION     DACDEG010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.128"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(52..522)
                     /locus_tag="IV142_002525"
     CDS             complement(52..522)
                     /locus_tag="IV142_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP8486116.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="IV142_002526"
     CDS             complement(681..1139)
                     /locus_tag="IV142_002526"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP8486117.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2455)
                     /locus_tag="IV142_002527"
     CDS             complement(1310..2455)
                     /locus_tag="IV142_002527"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP8486118.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2468..3385)
                     /locus_tag="IV142_002528"
     CDS             complement(2468..3385)
                     /locus_tag="IV142_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP8486119.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3813..4376
                     /gene="efp"
                     /locus_tag="IV142_002529"
     CDS             3813..4376
                     /gene="efp"
                     /locus_tag="IV142_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP8486120.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            complement(4516..>5471)
                     /locus_tag="IV142_002530"
     CDS             complement(4516..>5471)
                     /locus_tag="IV142_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8486121.1"
                     /translation="GTLELKVPRTRDGHFSPTVFERYQRNEKALIASMLEMYVSGVST
                     RKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKNYPYLMTDVLYIKVRE
                     ENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWITFFEYLKERGLQGTELVISD
                     AHKGLVSAIRKSFTNASWQRCQVHFLRNIFTTIPKKNSKSFREAVKGIFKFTDINLAR
                     EAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRR
                     REKIIRIFPNCVSANRLIGAVLIDQHDEWLSSSRKYIKFAK"
BASE COUNT         1653 a         1014 c         1037 g         1767 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc
     1861 cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt
     1921 gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca
     1981 gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat
     2041 aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta
     2101 tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc
     2161 catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat
     2221 gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa
     2281 tcagttcttc caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag
     2341 gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc
     2401 ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc
     2461 tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg
     2521 taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg
     2581 caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa
     2641 acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg
     2701 gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca
     2761 cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg
     2821 tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt
     2881 ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt
     2941 tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt
     3001 tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg
     3061 ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta
     3121 atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga
     3181 tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc
     3241 ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag
     3301 aacctttagg catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag
     3361 cttctgtaat tgtcgagata atcatgtggg cctcctaaaa aaagataata aaaaaaggag
     3421 ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa
     3481 gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt
     3541 ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc
     3601 cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa
     3661 agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg
     3721 agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt
     3781 gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg
     3841 gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac
     3901 ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat
     3961 acgacacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg
     4021 tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc
     4081 aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg
     4141 aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg
     4201 tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta
     4261 agccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg
     4321 aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg
     4381 ctaacggatt gataagaatt tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga
     4441 tctgcctttc aagaggtttt ttgtagtcaa gtccacaatc ctgtgtaaaa tactatacaa
     4501 tgctctcact attttttact tggcaaattt aatatatttt cttgatgaag aaagccactc
     4561 atcgtgttga tcaataagga ctgctccgat taaacgattg gctgaaacgc agtttggaaa
     4621 gatacgaata attttttctc ttctgcgcac ttcttgattc agtcgttcaa ttagattggt
     4681 actctttagt cgattgtggg aatttccttg tacggtatat tgaaaggcgt cttcgaatcc
     4741 atcatccaat gatgcgcaag cttttgaata ttttggttga tcgatataat catgaatcaa
     4801 tcgattttta gcctcacgag ctaagttaat atctgtgaac ttaaaaattc ctttaacagc
     4861 ttctctgaaa gattttgaat tttttttagg aatggtggta aagatatttc ttaggaagtg
     4921 aacttggcat ctttgccaac ttgcgttggt gaaggatttt ctaatggcag agactaatcc
     4981 tttgtgcgca tcagaaataa cgagttctgt accttgtaaa ccgcgttctt ttaggtattc
     5041 aaaaaatgtt atccaggtct cttcgctttc gccactttga atcatgaagc cgataatttc
     5101 acggtcgcca tctttggtta ttccaatcgc tatatgacag ctttttgaga gtactcgatt
     5161 ttcttctcgt acttttatat agagtacatc ggtcattaag taaggataat ttttttctga
     5221 taataaacga ttctgccact cgttaaccat tggttctagc tgttctgtta agctagaaac
     5281 gaaggactta gagacggatt taccacaaag ttcttccaca atttttgata ctttacgagt
     5341 tgaaacgcct gatacataca tttccaacat tgaagcaatg agggcttttt cgtttcgttg
     5401 ataacgttca aacactgtgg gtgaaaaatg gccatcacgt gttctgggta cttttaattc
     5461 tagcgtgcct a
//
LOCUS       DACDEG010000124         5273 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.129, whole genome shotgun sequence.
ACCESSION   DACDEG010000124 DACDEG010000000
VERSION     DACDEG010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.129"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(73..390)
                     /locus_tag="IV142_002531"
     CDS             complement(73..390)
                     /locus_tag="IV142_002531"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486122.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(417..623)
                     /locus_tag="IV142_002532"
     CDS             complement(417..623)
                     /locus_tag="IV142_002532"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486123.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(604..786)
                     /locus_tag="IV142_002533"
     CDS             complement(604..786)
                     /locus_tag="IV142_002533"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486124.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(800..1150)
                     /locus_tag="IV142_002534"
     CDS             complement(800..1150)
                     /locus_tag="IV142_002534"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486125.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(1176..1478)
                     /locus_tag="IV142_002535"
     CDS             complement(1176..1478)
                     /locus_tag="IV142_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486126.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(1481..1714)
                     /locus_tag="IV142_002536"
     CDS             complement(1481..1714)
                     /locus_tag="IV142_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486127.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(2700..3022)
                     /locus_tag="IV142_002537"
                     /pseudo
     CDS             complement(2700..3022)
                     /locus_tag="IV142_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3145..4260)
                     /locus_tag="IV142_002538"
     CDS             complement(3145..4260)
                     /locus_tag="IV142_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486128.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(4266..4664)
                     /locus_tag="IV142_002539"
     CDS             complement(4266..4664)
                     /locus_tag="IV142_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486129.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
BASE COUNT         1488 a         1079 c          696 g         2010 t
ORIGIN      
        1 aaccctcttg ttatttttat cacataatca ttttataaaa acgcttcgaa aatatcaaca
       61 tttttgaaga aactattttt ctaatttctc aatttcttcc aagggatttt cgctaatgac
      121 ccatacaggc acagattgct ctcgaggctc cccttcttct aaccaccaac ctgcataatg
      181 aacttcttcc tcctcagcat taccatgata ccaaccaaca aaggattcat ctacaacgaa
      241 tgaaatttct tcttctggat caaataattc atttttccat gcttcaattg cttgttcttt
      301 ggtgtacttt tgtttgttaa atacaccaca aatttcgtca tctacataaa aaatttcaaa
      361 atcaaaatta ctcgttcctt ttactggcat cttaactttt cctcctttga ctcacttcaa
      421 tagatatacc actcatgttc attccattgt ttactagtta attggagttt tatcttaggg
      481 ctttgtgcac tctttccaaa aacttgagcg tcgttttttt cttctaacca aaaaaggtaa
      541 tttcttacat tcggtactaa atgtttttcc tgcatttctt catgaaaaac atataattct
      601 gcttcatttt tcaaccaatt cattattcat tccctcattc acttcttcat accgccattc
      661 agatagtgag atttcttttt gtttctccac ttgtcctata aaaatcgctg tattcaacaa
      721 gctgatcaca cttaataata aagcaatgaa actaatggtt acacttactc tttcacattt
      781 tgtcattttg tttccttctt tactcgtttg ttatttcttg taataggtct aataactcct
      841 tcaccgaaat ttgcaaatgg ttcgcaaaaa tttcaataaa agcactcgca cacattacca
      901 gaccttcaac tgatatatca ctgcgacaat aaaattctgg agaaacactg atttctgatt
      961 ctttcttaca attgaacgaa aaaactattt ttgaatcaaa ttccggtaat tcttccacaa
     1021 ctatgttttg ggattctacc aagaaaggga gtgcctgttc taatgcttct tctggtgttt
     1081 gagcagatag aataattgct tcttgttctt ttatttcatt gttctcaaat actttataac
     1141 tttttttcat tgttagttcc tctttcattc ctctctcaat tagctaagcc gttactggta
     1201 cgccattctc taaatttagt atcgctaatc cttaatgctc gtcgaatatc aatcattcga
     1261 taacctattg cacgaagttc tttatatctt ttagcagtta gcgtctctaa cgtcatttca
     1321 ggcaatgggt tcgaatcaaa aatagaattc gaaccgatta tttgggccaa ttgttgtact
     1381 tgtaacagct cttctgaact taattcagtg attgctcgat tgcctatcat tgcatacatt
     1441 ttctgtcgaa tatacttctt ttcttgttta gacaacattt ctatccactc ctattttcat
     1501 tatcatttgt tacctccact tcagtaaatc cacaaataaa tgaattcata ttccaagtac
     1561 cattcccttc aactacaact tttctcctgt cctctttcgg atattcaaga atcagaccgt
     1621 ttaccaatat gtttttcact actactaatt tgtttgtata ccgtggaatt gctgttccga
     1681 tatacttcac tttatttcct acgctaattg tcatttcctc actcctattc ttttttatcc
     1741 aaaacttttt cctgtcctat tcctgccaac gaatatactt cactgcatgc ttaaacaaaa
     1801 caaaatgttg ccctgtttta tttccgtctt ttacttgttc cacatacagt tcatatttgg
     1861 tcacaccttt taaaacgcca gatacttttt ttcatccatt gtcacaatta gttttttcat
     1921 caatcgattg atactgcact gctcattttt cttgttcata tagtattcct ttctttttca
     1981 aaactagcct cttttgttta ctagttttca tcaatcatca acgatttaac agattttttc
     2041 tttcgcaggg tttctagcgt cagcaacccc aaactctaaa aaagtgcctt tgctcccttg
     2101 aaaaacgagc ggacgattgt actcgtcatc atatgttttt catactacct gatccacgca
     2161 aataaccttt ttttgttctg tgccacaccc atccatacgt atcgcttgag attcaccata
     2221 cttttttagg tggtttatcc ttcctaagtc cctgttagac acgctgatta acttagaaag
     2281 tcctgtttgt atccaatttt caaagatctc ccaaaagaaa taagtaggtt tacgtgtatc
     2341 gcttctgttc aaagtacctg tttgtacttg agtatttcta ctcccctcgt tcaagggatt
     2401 ccttccacca gctagctttt cctttcatca tcaaatgttc ctgaaaagca tttacgtttc
     2461 gtgtccgtgt gcttacttat tcttttttcg atagcgatta tccgctcgct actcgctatt
     2521 ttatactttg tttgttagct caaataaaaa ctaacaaaca aaaaaggcta gtttctttcc
     2581 gttttatttt tttaactgtc gataaatagt tgttagttta attccaagtt ctaaaaccaa
     2641 tacgcccaac aaaaccacag gaaaaacaat tggggcaaac aagtaaaatc ggatagtttt
     2701 catgtcttcc ctctttcttt ttggtgtcgc tgtggtcgtt cttgccgttc aatacttcga
     2761 tacatttttt attcattatt tgctcgtttt gttttcctct ggactgcttt tcttgctttt
     2821 tgaatacttt atacgctttt cgtgcctgaa ttaataggtc ttgtcggcgt ttcattcgtg
     2881 agaaaacaat atttgaaacc atcaaatcct ggttcgaaca accacgattc gcaataattt
     2941 tcccatcaaa aaaaatagtt ttcacttgag aaaaaaacag ttggaaatct tgttttgttg
     3001 ctaatttttt tgtcaaaatc actgtgcttt tccctgttga atctacaatg attcttcgaa
     3061 caatccgatt cgataacttc caacgttgcc gattaaacaa tgttacttca caacaaatat
     3121 gacgtaagtg attaggggtc attttcatgt gtccaattcc tccaaccaat tatgcatcga
     3181 aattttgggc aattgtgaca gcccataaat ctgcgtttcc aggtcttcat aactacaaga
     3241 acgcttggct tgaagtaatg aatattctgc ttgatctgat agttttgttg ccattggatc
     3301 tagattcaat tctcttacac aacattccca aaaattcaat agcgctctaa aataaaatcc
     3361 ttttcgattc tgaattgctt ttccttcatg acgagcaatt ttttcatttt gaatacaccg
     3421 ttgatacgtt ttgcttaaat cttctgcaaa tgtttcccct aaaattcgat actcgcttcc
     3481 atcagataaa ccatgtgttt gattatactt tcgttgccgc ttttctataa ttcgtttact
     3541 gtcgtatatc atgtttagct gctctttcgc ttcacgccat tcagctgaac tgcgaacaat
     3601 ccatgtcaaa acatctttcg gaacaaacgt actttcgctg taatgagcaa taaactcttt
     3661 ttgccaatca tcagccattt caacaggact agtcgtatcg atttttgtat ctagattttc
     3721 tgtatctaga ttgttagaag aattattaaa taattgtgca gaattttcac atggattttt
     3781 ttcgtcaaaa tcatcgaaaa tcgctaaatt atcaacagat tcctcctctt cttttccttg
     3841 tgcagaatct acactggttg agtcaacagg acctaatgaa tacacatcat tttctgtcgt
     3901 atctaattct cctagataca accgagcttt gtttcttttt cctgtttgtt gatcaaatcc
     3961 catatcctct atttctagta gctcatattc tttcaattct tttttgattg tattaacaga
     4021 acgtctgcaa caattcaatg cattggctaa ttcttgttcc gtaaattcaa aataaacaaa
     4081 accttgacta tctacttgat ttcgttgaat ggctaaatct aagcgtgacc gaaaaaccat
     4141 atacgcgatt tttgctttat cagacaatga gcgatatttt tcacttgtaa taaatacctt
     4201 tggaaattga tagtataatt cattgtattt acgagttgcg gtaaatcttt ttgttgccat
     4261 cgttattaca cctcatcttg tccatatatt aagaaagaat tttggtgttg gtagacacga
     4321 acatcttcca ttgtagcaat caattctttt agataaattc gaccgaattc aattgttagt
     4381 aagtcgtaat cttctaattc atttttgatt tcttcaattt cttcttcaga cacattgagt
     4441 aaaatggcta attcttgatt ggtataagaa atatagacat gtttcttttc atcaatccgt
     4501 ttttcaatta aggattcttc taagtactca ttcagtaagc tataggcgta ttttgcttgt
     4561 gcagtcatcg cataatattt tgacgatttt ataaagaata gttttggcat atgaaaagtt
     4621 gcttgtgtca gcacatctgt tgcgcgaaat ttgtttttta ccatattttt tcctcctaga
     4681 aaaacacgca aaaaaagctc tgtataaatc gaattacaca gagtttcttc ttgttgatat
     4741 ttaatttcta aaatacgttt cgggtaaaaa ctaaacactt actaatattt agattgtaga
     4801 atcttcgcct tcatttattc attttaaaga gaaaaagatc aagtgaaatg tgcccaaaag
     4861 tttgattcta ttaattttta aacaccattc ataatatatt tcctataatg taaaaagcgt
     4921 cgatacttat taaagcatcg acgttatttt tatagtaaat ttatcaaaat ttttgccaac
     4981 attttttccg atttttttcc ctatttctat ctttccttat ctttcttagt taatattatt
     5041 tgatgttaat atatcactac taatattaaa aaacattgat ttatcatagg ataaagagtg
     5101 atagtttaat acttaaagag acatattaat tttatttaaa aattgacatt aatgactctt
     5161 aatcagcggg tcgcgggttc gagcccctca cggcccattg ggtgccaaac ccacggaaca
     5221 ggtaaatgtt ggtgcgaaag catttcgaaa acatttacgc tgttcttttt tta
//
LOCUS       DACDEG010000125         5187 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.130, whole genome shotgun sequence.
ACCESSION   DACDEG010000125 DACDEG010000000
VERSION     DACDEG010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.130"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(23..238)
                     /locus_tag="IV142_002540"
     CDS             complement(23..238)
                     /locus_tag="IV142_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP8486130.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            462..1262
                     /locus_tag="IV142_002541"
     CDS             462..1262
                     /locus_tag="IV142_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8486131.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            1281..3149
                     /locus_tag="IV142_002542"
     CDS             1281..3149
                     /locus_tag="IV142_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8486132.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            3142..3633
                     /locus_tag="IV142_002543"
     CDS             3142..3633
                     /locus_tag="IV142_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP8486133.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            3621..4175
                     /gene="srlA"
                     /locus_tag="IV142_002544"
     CDS             3621..4175
                     /gene="srlA"
                     /locus_tag="IV142_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP8486134.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            4193..>5187
                     /locus_tag="IV142_002545"
     CDS             4193..>5187
                     /locus_tag="IV142_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP8486135.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         1815 a          825 c         1062 g         1485 t
ORIGIN      
        1 cttttttaat aattatagct ttctaatatc tttcatcaac ttttctagta atcattggga
       61 aaaatttcaa gcagtagatc gcacaaatac ctacgacgat ataaatgatc ttcgcaaaaa
      121 tagttgttga accaccagca atcatagcta ctaaatcaag ttcgaacaag cctaccaaca
      181 gccagttcaa ccctcccaca atcaataaag ttaatgcaat agcatctaaa gttttcatta
      241 gaattctcct ttcttaaatg ataaaacaat tatatatcaa aaagagaaac attgcttata
      301 taacgctcag gaattttagc aatctatgca aaaagatgaa aatttttttt gctcttccat
      361 tagtacacat tttttagctg aagctgtaaa tttcatgtct taattttttt tagtgagctt
      421 ttatactgga tttgtaagaa aaaagaagga gaggatcaca tatgtcggat tggttggata
      481 ttgcaggaaa agtagtaatt gtaactggag gttcctcagg aattggaaga agtatcgtcg
      541 aaaatttgtt gaagcagaat gcacaagtag cgaattttga tgtaactgaa tgccgtatac
      601 agcatgaaaa cctactatct ttaaaagtcg atgtgtcttc taaaacggac atagaagaag
      661 ggatatataa agtaatgaaa catttcaaga cgatcgatgg tctagtcaat aatgcaggaa
      721 taaatattcc ttctcttcta atagatagaa atcatccaaa aagcaagtat gaattatcag
      781 aacaagtttt tgataagatg atcgctgtca atcaaaaaag tgtatatttg atgagccaag
      841 cagttggaag aatcttggtg caaaagggat caggagtgat cgtgaatctt tcttctgaat
      901 caggattgga aggatcagaa ggacagagct gttatgctgc tacaaaagca gcgatgaaca
      961 gttttactcg ttcttgggca aaagagttag gaaagaggaa tgttcgtgtg gtaggtgttg
     1021 caccagggat actggaagaa accggtttga gaacacaaga atatgaagaa gctctttctt
     1081 atacgagagg tataagcgtc gaacagctga gaaacggtta cagtcgaaca agtacgatcc
     1141 ctctaggaag gagtggtaag ctgcaagaag tggcggatct agtctgctat tatctttcag
     1201 aaagatcaag ttatatcact ggagtcacga ctaatatttc aggaggtaaa acaagaggat
     1261 aaaggatagg ggggacgcac gtgacgctag taaacagatg gtaccaatta ttgaaattac
     1321 tagtcactca caagaaagtt tcaatccagg aagtgcaaaa agaaatcaaa accagtaatc
     1381 aaaccataaa aaaagatatt ggggagttga atgaagaaat tgccggtatt gcaaaaatca
     1441 tccaaaaaaa caaacatttt gaacttgaaa tttatgattt tgattttttt gatgagatca
     1501 tgcaaggaaa attgaaaaca gccagtgatt tcaattcggc atccaaaaga atcgcttatt
     1561 tactaaatag gctgatcgaa acagaggatt tcttacgtat ggatgatttg tctgaagaag
     1621 tcggtgtcag cagaggaaca gtggtcaagg atctaaagac attaaaagag atgatcaatg
     1681 attttgatgt aaagattaca ggcacaccaa ataaaggact aagaataata ggagatgagc
     1741 tagaattacg actactttta ttttattttg tttctcccta cttttcagag acgaaaacag
     1801 aacagtttct agaagaaaaa caagcaatca aacattttca gaagaagacg aatgctagta
     1861 aacaagaaat caccctttta acgaaagtta tcggtatttc tttgaataga atctctagta
     1921 actatctgtt gaagaagccc atcaatcgtt acagtgggtg ttttgaatat atccaagaat
     1981 tcaatgaact gatcaatcag ttggaagaaa tctatgaact aactttgagc caatatgaaa
     2041 aagattttat cagttttcct ttgaacatca tcaacacaag tataacgatg ataaggaaag
     2101 ctaactctga tttgcgctta tatttcgacc ccatgatgat gcgtattcga cgattagatc
     2161 tcattgatct ggatgagtcc ttcttatatc aagagatgaa acagcacctc tctttattga
     2221 taaatcgggt aatttttcgt tatcgattga ctgatttatt ttatggtgag atagaaaaaa
     2281 agtatccttt ttcttaccaa atcgctagcg aatgcataca agcattagaa gctatcttgc
     2341 aacataagat ctctaaagta gagatcagct acctaacgtt gtatttggag ttagcatttc
     2401 gatatcaaaa agagcatttt tcctctaaaa aagtagccat tgttcatacg acaggaaaag
     2461 gaacagcatt aatgatccaa agacaaatca aaaggatatt ggggagggat atacaaataa
     2521 cacatgtttc agaagaacaa tatgaaaaag tagatctaaa caaatatttc gctgttttta
     2581 caacgattcc gttaaaaaat gtaaatgaag agacaccggt gatccatttg actaatcttt
     2641 ttgatgactc ttggttaagg gcacaatggc aaaaagcaaa aaaaaagcga ttagccgggt
     2701 ttcattctac tgtttttttc tatcaaatat ggaaagaaaa aaagacgtat caagaatatt
     2761 taaacaaatt agtcgctcct ttgatcagag gctattatgt ggatgaagaa tttgaaaaaa
     2821 ggatcttgaa aagagaatat cagcaaagca catttgttaa caaagaattt gcttttcctc
     2881 acacaaccaa ccagctatct caacacattg ttttatcagt agggctaata actgatcaag
     2941 gtttagcaga aacaggagga aatggagtta tttttttatt tgccatacct gaagaaatga
     3001 cggctgaagt tgagaatgaa ttatttgagc tatacgatat cctatttgaa ctcataaaaa
     3061 tgggaataga tgagtttatt gaattagcac cgacacaaga atcattttta aaactaatca
     3121 aaagtaaagg agagagactt catgaatgat atgtatctat tcatagtttt cgcagcttca
     3181 gcttatgtgc tgcaagcagt tatcagttgg aagcaactaa agcatttcaa tcaaacatat
     3241 agcgaactgc gaagaaaagg aaaagtagcg attggtaaac gacctgggaa aatccgttca
     3301 ggcacgattg ttatgctagc tatagacgat catgatcaag ttttagaagt cagaaaaatg
     3361 caaggggtta catctttggc aaaatttcat tcattacctg tctatgcggg tgaagatatc
     3421 cattacatcg atcgttacca tccctcagtt cgagcggaag atcgtttgac acaagcagct
     3481 ttagaaaatg ccagagaagt atacttacga atagaggcag gaaatgaaaa aagtgaacaa
     3541 ccttcattgc cttttattgc tatgctcaat aattttcgga tatggaaaag tacgatcaaa
     3601 gaaaaaagga gtgacaaacg atggatatca taacaaaatt tgctgaggga tttatgaggc
     3661 tgttccaaac aggtgcagaa acattcataa gctggatgac aggaatcgta ccagttgtct
     3721 tgatgctgat ggttgcaatg aatacgctga ttgctttttt aggtgaagac cgagtaacga
     3781 aagtagccaa attatcagcg aaaaatccgc ttactcgtta ccttgtgtta ccgttcttat
     3841 ctgcttttat gttgggaaat cctatgtcat ttacaatggc tcgtttcctt ccagaatact
     3901 acaaaccaag ctattatgct gctcaagctc aattttgtca tacgagcaat ggtgtattcc
     3961 cccatattaa tcctggagaa ttgtttgtat ggatggggat tgctcaaggg atcgaaacct
     4021 tagggctaaa ttcgatggag ctggctatcc gatacttgct tgtgggactg ctgatgaact
     4081 ttattggtgg ttggataact gattttacta ctggatttgt atgcagacag caaggaatcg
     4141 ttcttagcaa aaaagtagag ctttcagttg attagagaaa ggagagtttt aaatgtatgg
     4201 tgatattaag atcgtaagag gaaacggcgg gtatggaggt cctttgatta ttaggccgac
     4261 aaaagataaa cacaagttca tctatgtcac aggtggaggt gagaggccaa tgatcgtaga
     4321 taaaatagaa gaactgacag gtatggagtc tatcaatggg ttcaaaacat caatacctga
     4381 tagtgaaatc gcgcttgcaa tcgttgactg tggagggact cttagatgtg gaatttatcc
     4441 taaaaaaggg ataccaacca tcaatatcgt ttccacagga aaaagtggcc cattagcaca
     4501 atatatcact gaagatattt atgtttcagc agtagggtta gaacaaatac agccactaat
     4561 ggaaactgat gataacaaag aaacagtaaa tactgcatct ccagataaaa agtacaaata
     4621 tgatacttct aaaaagataa ccgagcaaaa agcagagcag caaaactgga tagcaaaaat
     4681 tggaatgggc gcgggaaaag tagttgctac attcaatcaa tcagcaagag aagcagtaga
     4741 tacgatgatc cggacagtga tcccttttat ggctttcgtt tcccttttga ttggtgtgat
     4801 acaaggttct gggatcggta atgtatttgc taacattatg gcacctttag caggaaatgc
     4861 agttggatta acaatcattg gctttatttg ttcactgcca tttttatctc ctttgcttgg
     4921 tccaggggca gttattagcc aagtgattgg aacattgatc ggtgtagaga ttgggaaagg
     4981 aaatattcct ccccaattag cattaccggc actatttgct atcaatactc aaaatgcatg
     5041 tgattttatt ccagtcggtc taggtttgga agaagcaaaa gctgagacag tagaagtagg
     5101 tgtaccatca gttctttatt caagatttct taacggcatc ccacgagtta tcattgcttg
     5161 ggtagcaagt tttggattat atagctg
//
LOCUS       DACDEG010000126         5178 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.131, whole genome shotgun sequence.
ACCESSION   DACDEG010000126 DACDEG010000000
VERSION     DACDEG010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.131"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(44..706)
                     /locus_tag="IV142_002546"
     CDS             complement(44..706)
                     /locus_tag="IV142_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8486136.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(716..1531)
                     /locus_tag="IV142_002547"
     CDS             complement(716..1531)
                     /locus_tag="IV142_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8486137.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1793..2236)
                     /locus_tag="IV142_002548"
     CDS             complement(1793..2236)
                     /locus_tag="IV142_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP8486138.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(2370..2708)
                     /locus_tag="IV142_002549"
     CDS             complement(2370..2708)
                     /locus_tag="IV142_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8486139.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(2696..3073)
                     /locus_tag="IV142_002550"
     CDS             complement(2696..3073)
                     /locus_tag="IV142_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8486140.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            3297..4490
                     /locus_tag="IV142_002551"
     CDS             3297..4490
                     /locus_tag="IV142_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8486141.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4656..5078)
                     /locus_tag="IV142_002552"
     CDS             complement(4656..5078)
                     /locus_tag="IV142_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8486142.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         1573 a          984 c          888 g         1733 t
ORIGIN      
        1 ataaaaactg tcattcttaa ttgaaagttt caatttataa agtctacaga tccatagttc
       61 tcatcaattt acgattgttg aagtaaatca aaccaaatac gatagaagga cctagtagaa
      121 tacaccattg gggctttacg aaaaagaaga gaaacaaact tgtcaatagt aaaaaatcta
      181 gtaaactcac ataccatttt ttcaacgtat agtagaacgc tatccgtaga acttgatttg
      241 gtttcgacgc tggatttctc acctgaaaat accagtagtt gatgctaaag gaaactgata
      301 aacaagcaag taaaacaaac aacggaatca accattttcc ataagaaaaa cgtataaaaa
      361 agaaacaatc aaaaatcatt atcacactaa ctatccaagc aaatagccaa taaaaaaatc
      421 cctttagcca aaattggcga aacgtcttta tgaacaaacg tactggtgaa aaactttttt
      481 cttttgctaa ttcttctgtc atacctatca tcacgatgca acctggacca ataaaaagta
      541 aagaaaaaat aaaggaaaag acattccttc catctatcga caaaaggaac gcagctgcta
      601 tatacaagat attgacacac caaaaacaaa ggctacttgc caatgattga tacaaccaat
      661 aaaagaattc catataaaca tttttgtcaa aacgctgctc tttcataatc acctcctatt
      721 ccttcattcc tgatgaagcg atcccttcta caaaatattt ttgcatcgct aaaaatataa
      781 aagcaacagg aagaacagaa acaactgagg cagccatgat taatgcgtac tctgcatcaa
      841 atagcccaat aaacattttc aaccctaact ggattgtttt attttccgta gaagacaaat
      901 aaatgaatgg ccccatataa tcgttccatg tattaacgaa tgtgatgatc gtcaaactgg
      961 caatcgccgg ttttgtaaga ggtaaaatga tcttgcgata gattccccac tctgataagc
     1021 catcgatccg cgcactctca cataaagaat ctgggatact actgtagtat tgcttcaaca
     1081 agaacacacc aaaagcattg aacgcttgta atagcaccaa cgaccacaac gtatatgtta
     1141 aacccacttt tctcatcata atgaactgcg ggatcatata agactgccaa gggacagcaa
     1201 tcgtgccgat atatgctaaa aataaaatat cgcgcccttt aaaatttagt ttagaaaacc
     1261 cgtaagcagc aaaactagaa gtaaacaact gaatcaacgt gatcacaacg cttaaaaaag
     1321 ctgtattctt aaaaaatgtc actaaaggaa tacgagacca aataaccaga taattctcta
     1381 aatgccaagt tttagggatc cattgaatcg gaatcgtgaa cacttcatta tttgctttga
     1441 atgaagcaga aaccatccat aaaaaaggaa tgatcgtcaa taaagctaat acaatcagca
     1501 gaatcatacc caaccattga ataaagggca tgttttttga acgaattttt gctttttttc
     1561 tttcatttcg aacacctcca aagttataat cttcatttct ttgtaatgat tacaatccag
     1621 aacaacggtt attatagctg attcatttcc gttcttctta tgtttgtaag cgcttattta
     1681 acaagttatg taaatctctt tatttttttt gatttaaaaa tgaaaaaata caaaagcaag
     1741 actgtcatac gtctcgcttt tgtattgaaa tttcttcgat tagagatact tattactgtt
     1801 caaaagcttc tttgaaacgg tgaatcaatt cattgactac aggtgataaa atccgattct
     1861 ttttccagac tagcatgcag ttcgtttcaa tcattgggtc aagcggaacg aatgtcactc
     1921 gctttgaaag atgacttaca gcaccttcta tgactaaagc agcacctacc tgattctcaa
     1981 ttagcgcaaa tacattgaaa atcaaattgt acgttcctgt gatcagcaac tgctcctttt
     2041 ttatgcctaa ccagtcagca aacattcgct gtatttctgg acgctttgag atcaaaagag
     2101 gtacattcgc aaagtcttct tttgttatca cgtctttttt tgctaaaaca gaattttcta
     2161 aaatgagcaa tccccatttt tcctttcttg gtaacaaaat cgtttcgtat ttctctgtat
     2221 ctactggatc tagcaaaatc gctaaatcta acaggctttt ttctagtcac tccgtgatat
     2281 catcacttgt tccgctgtgg atatgaaatt gaacgctggg ataatcacgg atcaactttt
     2341 ctagtaatag tgccaatgta ttcgaattat cagcttccac acagccgatc gtaaatcggc
     2401 cgctgaataa ttgttttttt ctgttctcga attcttgagt ggtttgattc gttaaatcta
     2461 aaatttcttc tgctcggctt ttttagaaaa taacctgctt ctgtcaagat cattttgcgg
     2521 ttttcgcgta caaacagttc tgttcctagt tcctcttcta actccttcag ttgtcggctt
     2581 aaggttggtt gtgtaagatg tagaacttct gcggcttgag agattgttcc ttcttctgca
     2641 attgtccaaa aatattttag taagcgtaac tccatagcga tgctccttga tttttttact
     2701 tgatacattc cattttattt ctagataaca cacgatcata tttcttgatt tttccttgta
     2761 aacgttccaa acattcttgc atttcagcaa tttttttcgt caaaagttca ctttcttcaa
     2821 tcaaaagctt acgccgctca aatacagttg cttctccttc gctagatagg cgaatatatt
     2881 caatcaaaga ctcgatcgat aatccggcat ttcgcataca gatcgtaaag tcgacccatt
     2941 ttaggtcttc ttctgtataa tcacggattc cgccttttgt ccgattaact ggtggaatca
     3001 acccgattcg ttcataatag cgcaatgtat cggccgaaat gcccttttct tcactgactt
     3061 gtttgatatt catctgatca ctctttctct taatttcttg ccactttact tatgtatttt
     3121 atttattcta caactttaag tgtactccaa gtcaatactc atttctatta aaactaagct
     3181 ggattatcta tatcggttgt ttgtgtatta tttcaaataa aaaagtcttg tttttttatt
     3241 cttttttgga tatactttta acaatttagg gaaattttag gaaaaggggt ataaaaatga
     3301 caacgtatca gcgaaagtta ttattatcga catcggcggg aattgcttta gagaatatgg
     3361 atattatgtt tttagctttt tcattatctt caatgattac ttcttttcat attagtggta
     3421 cgcaagcggg tttcatttca acgatcacta atttggggat gctcattgga ggtatttttt
     3481 ttggcatcat agctgataaa tatggacgtg tgaaagtatt ttctcaaacg gtccttctgt
     3541 tttcgattgc ttctttattg atgtattttg cttcaaacat ctacttggtt tatctctttc
     3601 gctttatcgc aggtgtagga gcgggaggag aatacggggc gtgtatgtcc ttgatttccg
     3661 aaagtttttc aaaaaaacaa attggacgag cttcttcggt tgctggtatt ggtgcgcagg
     3721 tcggtgcggc tttggctgcg attttagctg ctgtgatcat tccaacgttt ggttggaaga
     3781 tgttgtatgt cgtaggtgta ctgccggtgt tgatggtctt gttcattcgt cgcggcttga
     3841 ctgaaccaaa ggaattcaaa tctgtacaat ctgccgaaaa gaaaaccaaa cttagcagac
     3901 tgtttgaatc aaaacatctt gcttggcaaa catgtggcct tagtatcatg gtaactgtcc
     3961 aaattgccgg ctattttggg ttaatgaatt ggctgccttc aattatgcaa aatcgattag
     4021 ggctttcggt ttctgggtct tcattgtgga tggtcagtac tattttaggc atgtctttgg
     4081 ggatgatgac ttttggcata atcatggata aattggggcc taaactagcc ttttctttgt
     4141 ttttaatttg ttcatcctta tctgtctatc tacttgtgtt agcccatagc cagtggagtt
     4201 tggtagcagc cgctgtgata gtcggctact ttatcaatgg catgtatggt ggatatggcg
     4261 cagtgattag tagtttgtat ccaacagaaa tccgcgcgac ggcgaataat ttcattatga
     4321 accttggacg agctgttggt ggtttttctt ctatcgtcat cggtttcttg atggatcatt
     4381 atagtttaac tgccgtaatc ttgtttttga gcggtattta cctagtcagt ctgctggtct
     4441 tgacaactct tactggtgtt acagcattaa gggaaagctt gcagcattga tagttgagtc
     4501 tgtaaatgac aagaaagagg tgtggcaaaa gcttgcatct gcggtaataa gaccagccaa
     4561 atcaaaaaca tgagacactg tttccgtttg gctggtcgta ttacttgatg cagggcagct
     4621 ttttcaactg ctaatttcct tctatctggc ggctactatt ctttgacgtc cgtcatttca
     4681 aagtacttta ctaataactt agcataacga aaaactgtgg atacactcgt atattgagcg
     4741 tctgcataat cttccaaatt tttctcagga tgttgataca tgaattcaat caaatgataa
     4801 ttatatgatt ttctgtagaa atgaagataa ataatgtaga ttgggaagtt ctcctgcttt
     4861 tccatagaaa agcgccgatt attctcattc ttcaaaatga ccttatcagg atatttttgg
     4921 aacaattctt ctaaatctgt tagataattc aagatagact tacttgatac cttcaattcg
     4981 ttttgtagtt cttctattga acaccaacgt tctgtttcaa gcagtaaatg aatcatttta
     5041 attttctttt gagaaacttt tgttaaaaaa gtctgcatat tttctcctcc tgtatctact
     5101 ttactatact acatacaaga aaaaaaacgg atacaaatat catattgtca caccgtcaat
     5161 agtgcataaa cctgttat
//
LOCUS       DACDEG010000127         5109 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.132, whole genome shotgun sequence.
ACCESSION   DACDEG010000127 DACDEG010000000
VERSION     DACDEG010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.132"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            70..1211
                     /locus_tag="IV142_002553"
                     /pseudo
     CDS             70..1211
                     /locus_tag="IV142_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1337..2416)
                     /locus_tag="IV142_002554"
     CDS             complement(1337..2416)
                     /locus_tag="IV142_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8486143.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2525..2965)
                     /locus_tag="IV142_002555"
     CDS             complement(2525..2965)
                     /locus_tag="IV142_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8486144.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3022..3432)
                     /locus_tag="IV142_002556"
     CDS             complement(3022..3432)
                     /locus_tag="IV142_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP8486145.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3613..4047)
                     /locus_tag="IV142_002557"
     CDS             complement(3613..4047)
                     /locus_tag="IV142_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8486146.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(4147..4995)
                     /locus_tag="IV142_002558"
     CDS             complement(4147..4995)
                     /locus_tag="IV142_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8486147.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         1866 a          979 c          849 g         1415 t
ORIGIN      
        1 gtaaacatat aggtagaatc tctactatct tatttcttgc atacaaaaaa atcaaggagt
       61 gagagagtta tgagatattc aaccatctta atatcaacat ttgcagcctt aagcttatcg
      121 cttactataa atcagacagt ccacacagag gagacagaaa cagccaaatg gacagaaacc
      181 aagtgggcca acgtcaatcg aattaccttt caggatgaat tttcatcctt caattcagga
      241 gtcgcaaaat taagaaaagg acaaacagcg accatcaaag caaaaattga ctattcggga
      301 gacttagcac ctatactaca atcagctgtt acatttgaga atgttgatcc cactttgtcg
      361 attggctatg acgagaacac tttgactatc caacacaagc aagcgatatt taccttcacc
      421 gttacattaa atcaggactt gttaaaacca gcgcttttta ctgtcaaggt atcagatgga
      481 ctaccgaacg atcatcattt aacgccgtat agtcagcggc agacaattga gcaagattca
      541 gccatcgact caggaggaga tcttgttgaa gaaccaacag ataaaccaga aaatgaaaat
      601 aagccagaag taccgcctac agaaaatcca gacggagaac agaaaccgga aatagaacct
      661 ggagaagaac ccgatacaga aactcaacca gagccagaca ataaatcaaa acctgaaata
      721 acccctggag aaaaaccaga tgtaactcca gaaccagata cggattctgg aaaccaaaca
      781 gtaccggaaa caaatccaga cacagataat gaaactgaaa atccagaaaa accagaggta
      841 gatccagaag aaaaaccaga tgtgactcca gaaccagata cggacgcaag agaccaagga
      901 ataccagaaa aaataaacaa aaaaacaatt caagaggatg gaaaaaaaga gagtaaaaaa
      961 tcaaatctag caattctaaa aataaatgaa gagcagctga ataaaaaatc acgaatattt
     1021 gatagcgccc aatcagctga aaccttaaaa agtagcaagg atacaacctt tgctgtcctg
     1081 aaacgaaaaa taaacagcta ccaaaatccg gagagtcaca aaacaaagtt atactgtgga
     1141 gcggaatcat tctattaagt atagcaacaa tgctttctgc aaaaagattt aaacaaaaca
     1201 gatctctcta acatttgaat aatgtctgtg aacgcatttt tcaaataaaa agaaatatcc
     1261 agaaaatgac cccaaaatac cagattgaaa tctgatattt ggggtcgttc atactttatc
     1321 tttcttcata tttattttaa gggatatctg tttcttttgg aagatggttc gggatctttt
     1381 tcactttcaa tacatctaaa aagaaggtaa agatcggaat gccgacaatc aagccccaaa
     1441 cgccaaagaa ccgttcgcta acaagtaaga ccacaaatgt ataaaaaatc ggcagttctg
     1501 ttttgctgga catcaacttc ggattcagca cataagactc aattagatgg acaatcaaaa
     1561 tcgtaatcag gatataaaca acatctcgga tgcctccttg ggaataagct atgaaagata
     1621 gaggaataca agaaatgata acacctgcta ctgggatcag acttaaaatg aaaatcatga
     1681 tcgctaaact cagcagctga tgaaagccaa atacagccaa ggcgattgta gtgagcaacg
     1741 tatttaaaag agcgacaaca aattgagctt ctaataccaa accaaatgta ttgacaaact
     1801 tatcggcaaa atagtaaata tcttgaaaga accaagaaaa ttcacttttt aaaaacaatc
     1861 ttgagaatac aattgttttt ttcttttcaa tcataaaaaa gaaacttaag atgaatgaca
     1921 tagcaaacgc cactgccatt tttcctatat cctgtaaata gccaagcgca atcgaagcgc
     1981 cattttgaag ctgttcaaaa aaatcgtttc gctcaatata agtatggata tattgtaaaa
     2041 caggatctgc atcttttggc tgatgctcgt aaaaatcaat aacggactgg atcatatcaa
     2101 atgtttgatg aataagcatc gggatatact ttgtgaatgc cagatagata aggtaaacga
     2161 tcagtgtata aatcatgata gctaaaacac ctgtagggat tcggacaaat cgctgaatga
     2221 tttgaacaag atgaatcatc aaataagtaa agataaatgt gagcaaaatg gttgtaatca
     2281 tacttctaac taagaaaaga acaaaaacga caaataataa aacagtgaga cggcgtattt
     2341 tttcattcgc caaaaaacgc tgataaacgg tcaaatcgac aacctccaaa taacaaaaaa
     2401 taaacacgtt attcattata tgatggaata tgaaaataag aaagagaaag agcaaaaaga
     2461 aaaaccgcta aatgacgtta tcttatgagt tggttttcct cataaaatcg ttcatctagc
     2521 agtttcagcc ctttttctgg tcttgatgaa aattatctag catttttcgg gtcagatgtt
     2581 gcagtgtatc caattcttcc tgttcaagtg gaagataaga cttgatcttt tgtggaatac
     2641 cttctgcttg tttttgcaac gcagcacctt gaggagtcag cgtaacattt acgattcttt
     2701 catcacccgt ttcacgctga cgattaatat aatttttttc tgctaacttt tttagtaagg
     2761 gagtcagcgt accagaatcc agacacaaat catttcctag ctctttcact gaagtctttt
     2821 tcttttccca taaaactaac atcacaagat actgggtata ggtaagattc agcggcttga
     2881 ggagggaagt gtagtgtcta atgatttcct tagaggcggc atataaagga aaacatactt
     2941 gattaactaa atatagatct tccatttttc cacctccgct gttttactat agtgtatcat
     3001 agcggattta cacaaaaaag tctattcact aactgctttc agagtaactt ctatatttcc
     3061 agctgtagct tttgaatacg gacacacttt atgcgctgcc tctaataatt cttgcgtttc
     3121 ttccagagaa agaccttcga tagatccttc aatctctaca cctaagacaa agctcgtttc
     3181 actttctgaa taaagggaaa cttgtgcact gacagtagaa gccccagaga tatttttttg
     3241 ctgtttgatc aattctaatg cactgttaaa acatgcactg aaaccagctg caaacaattg
     3301 ttcagggttg gttgcatttt cgacgcgttt accaggagct gcaatattca attcgaatgt
     3361 acgatcggga ttatgaactt ctccagagcg tccgcctgtg ttgatcattt tcgtagaata
     3421 catttttttc ataaaattac cacctttaat tatattttat acaattgaat tttacacaat
     3481 ataattgtgg aaagcaaaga aatggattgg cataagataa ataagagaaa gcacatgaaa
     3541 cagacgcgct ttctcgaata gcaaacatat acaaaaaaat atcagagaca aaaactaatc
     3601 tcatcataaa cattacatat atttctcaaa aagttgtttt ctttcttcaa aacttacttc
     3661 acgatggacc tgatgaagca tccataaata accaaaagga tctctgaaca ttgagttagt
     3721 cacaccaaag tccatcattt ctgtcatttc ttgaatctct acacagccat ttcgtaatgc
     3781 tttattatgt gtgtcctcga tatcagagac gatgatattg aaccagatcg tatttggatg
     3841 ttcagcagta ggagcagtca aaccaagaga cgtgttttca tccagcatat ggaatctcgt
     3901 gccatacaag gtaaaaatga cttcattatt gcctctaggg taatctgttg cctcaatcag
     3961 ttgtatttca tcaaagattg tttgatacag ttctagtgct ttcaaactat catttaatac
     4021 aaaatctatt tccgttcctt tatgcataaa gatctcctct ccttttcagt cgaacagtta
     4081 ctagattctt ttattatacc agttaaagaa aacgcattca aaaaaatagc ggatttactt
     4141 accaactcat tcgattggat gataagtaaa tccgcgtccc attacttctg ctacgctgct
     4201 gacaatgaca aaggcacttg gatcgatttc ttctatcaat ggtttcattc gggaaaattc
     4261 atgatcagtc acaacgatca ataaaacctc ttttgacttt cgattatacc cgccttttgc
     4321 ctctagcagg gtaaggccac gtccaatctc agcacttaag cgaaggcgga tgtcttccaa
     4381 ataatgttca ctcatgacca taacagattt ctttcgattg atacctgttt cgatataagt
     4441 catgacaatt gaagtgattg caatagataa aatagcataa agaaactctt ctattccaaa
     4501 aacaaacaga ttaagagaaa caacgaatgc gtctgtaaaa aataagccaa tcgaagtatt
     4561 caagtggaaa tattttttaa aaatgagggg agggatcgtc gtaccgccgc tagaagagtt
     4621 gtttttatat aaaatcgcaa ctcctaaagc aaaaatcgca cttccaaaaa tgacagacaa
     4681 caaccgatct gaagtaatca ttgtttctgg aactattgca atgattaaag gaaaaaacaa
     4741 gcttccgaaa agtactttaa aaaagggttt tttacctaaa aatagaagag ctagtaccaa
     4801 cataacgata ttcaacacta aaataactat agctcggtca aatcctaaaa cagcttccag
     4861 taatattcct agaccgctga cacctccagc agcaatatga tgaggcccta aaaacatatt
     4921 gatagcgact cctaatatga agagggcaaa aataatctta atgtatgtcc atataaattg
     4981 tctcgtttct ttcatttatc cacttcctta attcattaat aatactctta tcttattata
     5041 acttactttt caccttaagt taactttaat taataaaaaa attaggctag tctaaattat
     5101 tatgtttca
//
LOCUS       DACDEG010000128         5053 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.133, whole genome shotgun sequence.
ACCESSION   DACDEG010000128 DACDEG010000000
VERSION     DACDEG010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.133"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            151..837
                     /locus_tag="IV142_002559"
     CDS             151..837
                     /locus_tag="IV142_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP8486148.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            839..1783
                     /locus_tag="IV142_002560"
     CDS             839..1783
                     /locus_tag="IV142_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP8486149.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            1982..2626
                     /gene="pcp"
                     /locus_tag="IV142_002561"
     CDS             1982..2626
                     /gene="pcp"
                     /locus_tag="IV142_002561"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP8486150.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            2705..3982
                     /locus_tag="IV142_002562"
     CDS             2705..3982
                     /locus_tag="IV142_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486151.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(4166..4915)
                     /locus_tag="IV142_002563"
     CDS             complement(4166..4915)
                     /locus_tag="IV142_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP8486152.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         1636 a          877 c         1070 g         1470 t
ORIGIN      
        1 tttaccttac aataagtaag ataatttttg tgataaaaaa ataacgaaaa atatttaaaa
       61 atagtgtcta tttcgtgaga aaacaactat attttttatg tgttttgctt tataattaaa
      121 aaaatattgt cattttatgg aggaaaatat atggaaaatt atcttagcct tttaggcgtc
      181 ttgattgtta ttctcgggtt tgcatttaaa ttagactcga tattgatcgt aatggttgct
      241 ttagttgtta cagctttgac tggcggtctt ggcattcaag gaatgttaga acttttaggt
      301 acaagttttg taaacaatcg cgggatggcg atttttatca tcattatgtt ggcaacagga
      361 acgcttgaaa gaaatggtct gaaagaatca gcggcttcct tgatcaagcg attcaagaaa
      421 gtatctgctg gtatgattgt agatatttat gggatctttc gaatgatctt tgcggctttt
      481 aacgtgagtt ttggtggagt tgctggattt gttcgtccaa tcattttacc gatggcattg
      541 gggaccatcg aatcaaaagg gttagaagtc catcctaagc atgaagaaga actaaaaggt
      601 atggcttcag ctatggaaaa tgtctgctgg ttctttggcc aagtactatt catcggagga
      661 gcaggggcgt tgctagtcca atcaacactg aaagatctag gatatgacgt aacattggga
      721 cagcttgctt tagtggaagt tccagtggct atcacggctt tagttgtggc aagtgtctac
      781 ttctatctta aagaaaagaa attagcgaag aaatattatc caggaaaggg gcaataagat
      841 ggactttttt acaaatcctg atattttatt aggcgataaa ttactggaga tcctttatat
      901 cgtgatggga ctcgtattga tttacactgg agtaagaaat ttaacagata aaaccaatcc
      961 tcatcgttat gggtcagcct ttttctggac ggtcttaggg gtagttattg caggaggaag
     1021 atggcttccg gcaatcgtga acggtggctt gatctttgcg atgaccatac cagcaattgc
     1081 taagaaagtc agcaaaggag aaagccggct tccatcaaaa gaatatatgg aaaaaatgtc
     1141 agacaaacta ggaatgaaaa tttttattcc agctttgagt atcggtgtct ttgcgatctt
     1201 gtttgctcta tttaccaatt taggtgcttt agtcggcgtg ggcgtcggtg tttttgcagc
     1261 catgctaatc ttgatgttct tttctcgaga taatcgacca agtacatttt tagatgatgc
     1321 agcagatatg ctgggcacag tagggccgtt aagcatgttg ccaatgcttt tagcttctct
     1381 aggtgcggta tttacaagcg ctggagttgg gacagtgatt tccaatgcag taggtacgat
     1441 cgttccagaa ggaaatgtga acatcggcat catcatttac gctcttggca tggtgatatt
     1501 tacggtaatc atggggaatg catttgcggc catcaccgtg atgacggttg ggatcggtgc
     1561 accattcgta tttagtcatg gagcgaatcc agcgttaata ggaatggttg cattgacttg
     1621 cggtttttgt gggaccctgc tgacaccgat ggctgctaac ttcaatatcg tgcctgtagc
     1681 gatgctggag atgaaagaca aatacggagt gatcaaaaac cagttgttta ttgcgttatt
     1741 tatgctagtt ttccaaattg cttatatgat tctatttaaa taatagaaga gaggggttgt
     1801 gacagaataa ttgtcgcaac ctcttctaac attgtagtgg aacactattt ttttataaga
     1861 atatattaag tttgatctgt tatgttattt aaactattgt ttcctttatt tacgctgttc
     1921 aaggaaaatg gtatgataaa agataaccat atgttaaatc aatcggacgg aggaacaaca
     1981 gatgaaagta ctagtaacag gttttgatcc atttggtggg gacaaagtga atccagccta
     2041 tgaagcagta aaaaaaatgc cagatgaaat cagcggtgct gaaatcatca aagtggaagt
     2101 accgaccgta ttcgaaaaaa gcagtcaagt agtcaaagaa gccatccaac agcaccaacc
     2161 agatgtcgtg atctgtgtcg gacaagcagg tgggcggtca gcagtctcgt tcgaacgtgt
     2221 cgcaatcaat ctagcagaag caagaatccc tgataatgaa ggaaatcagc cattcgatac
     2281 ggctttagaa gaaaacggtc cagccgctta ctttacctca ttgccaatca aagcaatgac
     2341 aaaaaatgta caagatcatg gtctaccagc gtacatctct tatacagcag gaacatttgt
     2401 ctgcaatgat atcatgtatc gtttgcttca tttgatcgaa acacaattcc cggcaattcg
     2461 aggtgggttc atccacgtgc cattctcacc cgatcaagtg atcgatcgtc cagtgggtac
     2521 gccttctatg tctttagaag acattgcttc ttctttgact tatgcagttg aagcagcagt
     2581 agaaaataaa gaagatattt ccggaaatgc cggaacaact cactaattta gcaagaaaca
     2641 ctggctggtt gctaacgtta tgttagtagc cagctttttg taattagaaa ggaaagaaac
     2701 agccgtggaa gaatggcaat ccgtctttga agaatggttt ccaaaagaaa tcagcaaaag
     2761 ctatcccatc aaaatttcta aacaatacac aagcagtcaa cgttgggaga tctatgcaaa
     2821 attaacgaaa aaacagcggg aactagtcga taagcaccgc cgatatctaa tcagctctcg
     2881 ttttatggaa gaacattatt tagctgcaac agattgggtg ttttctgact tcaagatcaa
     2941 tccgtttttt cgaacaaaac gaagccagca aaaattgtat tgtgagtgtg gtagagaatt
     3001 aaaagtgcaa tacatcgtta aatcgccaaa aaccggaaag atcctaaaac taggcatcaa
     3061 ccactttgcc gatcacctgc atgtctctcc gacagtggca gcttcgatcc atcagggaat
     3121 gacaaaagtt gatctggcct tagacgaatt attatggctt aagcagaaga atatcgattt
     3181 tcctgaagga ctatggcaaa aatactgttt tgtcttgtac caaaatcgtc ggatgaaaca
     3241 gccatattta ccggatataa aattagctca gcggctcgca gaatttcgcc aagtagaaat
     3301 gccgatttac atagcggatt atcaagcatt ggaaaatgaa atcaagaaaa taagtgaaca
     3361 catcaatggg cagtcaaaaa agcgacagat aaaaaaagaa ctatttgacg actttgcaga
     3421 agaattagta aaggatgtag aagaattctt gatcaactat cgcgcatttt tacgtaaaga
     3481 ttggcaatcc atcgtatatg aagaagtgcc agtacatcct aatgcttatt ttgaaacatt
     3541 tatttccgtg ttgagaaaga caaaacgtca acgaacacca gaagtcactg ctcagatgga
     3601 atattttgcg aaaaaccaac gattcatcca accaaaaatc tatctattta tttggaagca
     3661 atattgtcgc tatggtttta cagaagggtt cttcgacagc attcctcgga tagtcagaaa
     3721 tggtttttta aaagtattgc gtaaagaacg agaagctata caatctgctg ataagaaaga
     3781 ccgtacagtc tcaaaagaaa aatggcaact agtagtgaaa gatatccaat ctggaaatgt
     3841 gcaagagaca atcgacaaat ggaaaggaaa acattatcgt tttactgaag ctcagaaaca
     3901 agcgttggaa tattatcaga agttagaaga atcactgcgt ttcaatgatg aggcaagaaa
     3961 gtatctgaag gaattactat aaaaaataga ggctgggaca gaagcgttta actccaagaa
     4021 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcagc ttatttccga
     4081 aggagttgct tctgattccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4141 tacttttgtc ccacgctcag tttttttatg cgtgttgttg tgcgttgtag tattcttctt
     4201 ccattttttg catacgttca cgttcttttt ggttatacca aggtgaaacg gtaaatgcaa
     4261 ggacatcatt cagcaagtaa acagagctgt tcacgaacat cgccaaactt gcagatccat
     4321 gcgtaaatga aatataccaa aggaccattt gagcaagccc tgaagctaac caccagtaat
     4381 attggttgtt gtaacgcaag aaactgatga taccagcagt caagctaata gaaaaagcaa
     4441 tggcatctac ccacggccga ggatcatctg tataacgacc aatcaggaat cctgaaacaa
     4501 gatagacaag gaaagtactg atgattgcga taaaccagct ttttccagtg aattttcgga
     4561 tttttgaagc catattatga ttccattctt tacttaacaa tacggggata tccaatgtga
     4621 tcatataagc aatctgttca ccgatactta aatagttctt tgctgaaaac ccaacgataa
     4681 taaaacaaat cgcagacaaa atacctaaaa taccgttgac tgattttgcc gcattgatgg
     4741 ataaaataca caatacacca agcgtggtac cgataaatgt aataacggac agccaagtga
     4801 tgggactatt gacaagagtc atgacctggc agccaagact gaaaaagaaa agataataat
     4861 tttgttgcgg ccagcctttt agctgatgag ctaaaaactg aaaatatgac ttcatgtatg
     4921 aggactcctt aagacactaa atgtagtgtg ttctgtattt actaacacaa tctattgtat
     4981 gcttttcttg aaaaaaaact agtctttcaa ttataaataa tttgtgttat gataattttc
     5041 tgtttgtttt ctt
//
LOCUS       DACDEG010000129         4949 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.134, whole genome shotgun sequence.
ACCESSION   DACDEG010000129 DACDEG010000000
VERSION     DACDEG010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.134"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(136..1284)
                     /gene="nagA"
                     /locus_tag="IV142_002564"
     CDS             complement(136..1284)
                     /gene="nagA"
                     /locus_tag="IV142_002564"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP8486153.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1360..2196)
                     /locus_tag="IV142_002565"
     CDS             complement(1360..2196)
                     /locus_tag="IV142_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8486154.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2380..3597)
                     /locus_tag="IV142_002566"
     CDS             complement(2380..3597)
                     /locus_tag="IV142_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8486155.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            3980..4063
                     /locus_tag="IV142_002567"
     tRNA            3980..4063
                     /locus_tag="IV142_002567"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4014..4016,aa:Leu,seq:gag)
     gene            complement(4303..4881)
                     /locus_tag="IV142_002568"
     CDS             complement(4303..4881)
                     /locus_tag="IV142_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486156.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1522 a         1017 c          792 g         1618 t
ORIGIN      
        1 tttacattgt tacttattga acaataggaa attaaagaaa cttttgaaac ttatgtaaag
       61 tgatttttac tagaacacaa aaaaacctta aaaccaaact taagaagttt agttttaagg
      121 ttaacttaaa ttatattatg cgtggaaacg ttccacgcca tctagataag ttgcttccaa
      181 tgtcatatca gggttcaata cgataaagtc tgcatcgcgt ccttgagcga tttttccaca
      241 tgtatcatcg attttacagc tgattgctgg aatcagacta gccatcatga ctgcttgttc
      301 tggtgttgct aatccccagt ctacaacatt tttaattgct tctttcaatt tcaatatact
      361 tccagctaag ttgccagatt ctagacgtgc tgttccttct tttactacta ctggaaattc
      421 gcctaaattg taatttccat caggcatccc accggccatc atacagtctg taattaatgc
      481 gacatgatca tgtcctgctt tttccattag gattcccgca gcatttggat gtacatgatg
      541 gccatcacaa atcaattcag aaaagacatg ttctaaagat aataacgcac ccaccatacc
      601 tggttcgcga tgattcaatc cacgcatacc attgaaagca tggacaaata cacttgcgcc
      661 tgcttcaaca gctttttgcg cttcttctag tgtagcatta ctgtgtccta gagaaacaac
      721 tactccttct tcagtcactt gtttgacaaa ctcttctaca ccttgacgtt ccggtgctaa
      781 agcaattttt ttgattaaac cacctgaagc ttcttgccat tcatggaaag tatccaaatc
      841 aggatccccg aaataacttg ggttttgtgc ccctttgtgt tcttctgtga agaaaggacc
      901 ttcaaaatag attcctttga ttttagctcc tggtgcttcc tgatacattt tgccgattgt
      961 ctcagctaca tcttttaaac gttctttgct tgacgtcaat gttgttggta aaaatgaagt
     1021 cactccacaa gataacagac cttcagacat cactttgatg ccttctgcat cattatccat
     1081 tacatcatga ttcatatagc cgtgaatatg tgtatctacc aaaccaggag caatccattt
     1141 gccgctttgg tcaatgatgt tgacttctcc ctcaggtagt tctttggtgt attcaccaaa
     1201 aagtccatcg gtaatttcaa gatagcctgc atgtttgaca cctgacgcta aaaagaattt
     1261 gtcagcatag ataaaagttc tcattctatt ctctcccttt gtttttctac ctctaaagta
     1321 ctcttattta gagaagaagt caagaatggt ctacaccatt tacaaatttt ttgaaatttg
     1381 tttcacagct tgtgctaagc cttcatagaa tgctgctctt tctggttctt ctttacgctt
     1441 gtatattaag cttttaagca caaataaatc attgaggtaa tagtagctat tcttctcttg
     1501 agcccaatag attccttgat tcacctgttc taacgcttcg tcgatttcat tttgatggaa
     1561 taagaaattt ccataattat aaaaaatttg cgaaaattca ctttttgatc gttcatcagc
     1621 tgattgatgc agcagttgga tgcttcgact caaatagtag cgtgcttcag ccgtttttcc
     1681 tgccacacca tatatttttc cgatacaact aatcagttgt atttctgtgt cagaaacatg
     1741 gattctttct ttttggctag agtagcttaa tgcttctttc aaataataaa gggactgaga
     1801 gatattttga cgcaacgtga attcgcaaac accgagataa taataatagc gttgaaagtc
     1861 agttgcttga tacagatttt cagaaatatc agaagatttt agtacttgtt ctaacttccg
     1921 ataatcacgt ttttggaaac aactatccaa cagttcaaat aatttatcgt ttgtccgagt
     1981 agtttttcga gagagattca tcacatgatc aatcgttacc cctagccgtt gacaaatctg
     2041 catcatcacc aatacattcg gcagttcctc attattttcg atccgactta atacactttg
     2101 tgaacaaata tcatgcgaaa gcattttttg cgtcattttt tgttcttttc gaatctcttt
     2161 aatgatcgtt ccaaaggact cgattaacgt ttccataagt atcctcccca aaatatgcgt
     2221 attcgcattc tattcgtatt ttactttagg aagtcgcaaa cttcaagaaa agacttgcaa
     2281 ctaaataata tactttagtt atttaagaaa actccactag aaagagagcc tcagtgtgtt
     2341 tgtctgtggc cctcctactg aaaaatttgg tttttaaaat catttttggc gataggttgc
     2401 taggaattct ctcattttct tagctgtttg tttcaaatca tccattttag gaagataaac
     2461 gattcgaaaa tgatctggct ggttccaatt aaatccacca ccatgtacaa gtagaatatg
     2521 atgttcatgt aaaaagtcca aaacgaattt ttcatcatcc aaaatattga atcgctttgt
     2581 atcaattttt gggaatatat aaaaagcagc ttttggtttt acggctgaaa gaccaggaat
     2641 atcattgatt gcattataga tatattctct ttgttcatag atccttccgc ctggcaggag
     2701 cagctcatcg acactttgat aaccgcctag cgcggtttgg ataatttgct gagacaatac
     2761 attggaacac aaacgcatgg aggacaacat attcagccct tctatatagc ctttgacgtg
     2821 tgacttatct ccacttagga ccatccagcc tactcgaaat cctgctacac gatgagattt
     2881 ggatagaccg ttcagcgtaa caacgaacaa atcaggcgct aatgtagcaa tcggaatatg
     2941 ttctagacca tccatcacta agcgatcata gatttcgtcg gaataaataa tcaggttatt
     3001 ttgtcgagct acttcaacga tttcttctaa taattctttt ggataaagtg ccccagtagg
     3061 attattagga ttaatgataa caatagcttt tgtccgactc gttatttttg atttgatatc
     3121 atcaatatca ggataccatt ctgcctcttc atcgcaaata taatgaacag gtgtgcctcc
     3181 agctaatgca atagaagccg tccacaacgg atagtccggc attggaacca gtacttcatc
     3241 tccattgtcc agcaacccct gcatacacat cgtgatcaat tcgctcactc cgttgccggt
     3301 gtagatatca ttgatcgtca cattcggaaa atgtttcagt tggcaatatt gctcaattgc
     3361 ttttctagca gaaaagatcc ccttggaatc tgagtagcct tctgagtcac gggcattcat
     3421 gatcaaatca cgaatgactt cgtttggtgc ttcaaatcca aaaggtgcgg gatttcccgt
     3481 gttcaatttt aaaatactaa tgccctcttc ttgcatccgc tccgcttctt ctagaacggg
     3541 accgcgaaca tcgtaactga caccttctaa tttgttggat ttttcaaatt ctctcatcac
     3601 ttagccaatc cctcatttca aaaagttaga actttccaaa ttttttgtta tttactattt
     3661 caatatcata ctccatccta gaaaattttg ccacttattt aggaaaaacg taagtattaa
     3721 aaaaacacaa acaagcgttg agaacttaat tgtcatgata gtcattccat agattcgctg
     3781 cccaatccat tgcaattcca caaatgattc ctagcataaa accaagcaaa aaacgtccga
     3841 atgcaagtcc aatcaataca cctaagatca ttcccagtcc tgaaaattct ccgtcatatt
     3901 tctccatctt aataagtcct cccatttttg agcttgacaa ctatgtaaaa cctcgctatc
     3961 attagtgtgt tattattacg cagatgtgtt ggaattggca gacaagcatg attgagggtc
     4021 atgtgagcgt tagctcgtgt gggttcgagt cccatcatct gcactacaac tcgtgaagtt
     4081 aattttgctt cacgagtttt tttgtttgaa cgtttcttta tgccattata tacatatcac
     4141 tgtatccttt acaaaaagaa cgtacaatca tgtaaacagc ctgaggtgag tatatgaagg
     4201 tcggaaacaa gctactcgta gaaacttgca agtgttaaaa caattaatcg aaaaacgagt
     4261 aaaatccctc acctaatact aaagcgaggg attttttgta tattatctgt ttatcaccgt
     4321 tattataaag gtacaaatac caagcacggt taatccaaat tgcataaatt catttttttg
     4381 tacactcatg cttttcatta gctctttaac tgccattagt actaagatgg cgataacaac
     4441 ataaagtata tagtacgtcc gcattccgga agcccatact gtgggagaaa atcccataat
     4501 taaccttgaa caaaatccca ttatcatcaa aataactaaa aaaatagaag ttttcttatt
     4561 gtcaaaactt aaataaattc caacaattaa acatagtaat aatgccaaga ctactaaagt
     4621 agctatccaa gtacctggat aaaaaagtga cagtttcgtt cctaactctg taaagagatt
     4681 attcaggtta ctactactcc aaatcattgc ccttttattt ccattgacgt aagtaaagct
     4741 ttgacccatt gtattaccaa aataaataat caggtttaag aaaaacggta atgcagtcaa
     4801 tatacgcaca taatagtttt gacatttcct atatgttaga aggaaaatta agaaaaatag
     4861 caataaaaat aagatattca tgtctaaaaa aaacggcttt ccaaatgatg aaatacctaa
     4921 atctaactta ttaagaaagt tgaaatttt
//
LOCUS       DACDEG010000130         4778 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.135, whole genome shotgun sequence.
ACCESSION   DACDEG010000130 DACDEG010000000
VERSION     DACDEG010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.135"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(207..1169)
                     /locus_tag="IV142_002569"
     CDS             complement(207..1169)
                     /locus_tag="IV142_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8486157.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="IV142_002570"
     CDS             complement(1171..2610)
                     /locus_tag="IV142_002570"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP8486158.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="IV142_002571"
     CDS             2795..4741
                     /locus_tag="IV142_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP8486159.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1517 a          820 c          790 g         1651 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaggc acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttcca
//
LOCUS       DACDEG010000131         4765 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.136, whole genome shotgun sequence.
ACCESSION   DACDEG010000131 DACDEG010000000
VERSION     DACDEG010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.136"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(81..1889)
                     /gene="pepF"
                     /locus_tag="IV142_002572"
     CDS             complement(81..1889)
                     /gene="pepF"
                     /locus_tag="IV142_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP8486160.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2065..3063)
                     /locus_tag="IV142_002573"
     CDS             complement(2065..3063)
                     /locus_tag="IV142_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP8486161.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3289..3942)
                     /locus_tag="IV142_002574"
     CDS             complement(3289..3942)
                     /locus_tag="IV142_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP8486162.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4274..4672)
                     /gene="spxA"
                     /locus_tag="IV142_002575"
     CDS             complement(4274..4672)
                     /gene="spxA"
                     /locus_tag="IV142_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP8486163.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1398 a         1003 c          743 g         1621 t
ORIGIN      
        1 aaaaaaatac gtcgactccc ttacaagaga gccgacgtat ttttttcgtt tctattcgag
       61 aaaatcaaaa atagaaagcc ttataaacga tcgatcaatt cttctaattc atttagacgt
      121 tgttcaaaca tcgacattgc atcttcaata tatgcagcct gtgtcatatc aactcctgct
      181 tttttcatta cttccacagg ataatcgctg ttacctgctt ttaggtaagc caagtagttc
      241 tctagtgcct ctggttcctg gtttagtatt ttcttcgcca gtgctgaagc agcagaaaag
      301 ccagtcgagt attggaaaac atagtaatta taataaaaat gcggtatccg tgaccattcg
      361 aatttaattt ctgggtcttc ttcgactgct ggaccataat attttgcatt caatttacca
      421 taactatcac ttaggtattc actagtcaat ggcacacctt tttcatcttc cgtatgcatg
      481 aaatgctcga attcagcgaa ttgtgtctgg cggaaaactg ttcctttaaa cccatccaag
      541 taatggttga gtacataagc ccgtacacga gggtcttttt ctgtttccaa taaatactcc
      601 gttaggatat tttcattcgt tgtcgaagca atttctgcta aaaagatgga gtagtcgccg
      661 tacacataag gctggttcga acgagtgaaa taactatgaa cactatgtcc catttcgtgg
      721 acaagcgtaa atagctgatc cagcgtatca tgccaattca ataaaatata tggatttgtg
      781 tcatagcttc ccgaagaata agcaccgctt cgtttccctt tattttcgac aacatcgatc
      841 caacgttcag agaatgcttt ttctacgatg gccatgtatt cttcacccat tggtttcagt
      901 gcttctaaag ctttttcttt tgcttcttcg tacgtaaagg taattggagc ttcccctaag
      961 accggtgtat aaagatcata catgtgcagt ttttccactt ctaataaacg tttccgtaat
     1021 tccatgtatc gatgtaacaa aggcaaatgc ttgtttacca cgtctaccaa agtatcgtat
     1081 acactttcag gaatatgatt attgctcaaa gacgcttctc tggcagagct gtaattacgg
     1141 actttcgctt taaaattatg tccttttata tgtgtgccta aagtagaagc aaatgtattt
     1201 ctaaattgtt cgtaaacact gtacaacccc ttaaatgctg cttcgcgcac ccttcgatcc
     1261 gtgctttcta gcaactgacc atacacgcca tgagataatt ggactatttc accattttct
     1321 ccttcaatcg ttggaaaaac tagatctgca ttattcaaaa cagcaaatgt atcacttgaa
     1381 gcatcaaaga tttcacctgc tccagcaaga agagattcct gctcagcaga taagacatgg
     1441 gctcgattat ctactatttg ctggatataa tggcgataga cttccaattt tggttcttct
     1501 ttgaaatatt gccaaatctg gtcatctgac aattgcaata tttctggttc aaaccaggaa
     1561 acggcttcac taactttcga aaacagactg cttgctcttg cataaagcgc ttggtaatct
     1621 gtatttccag tgtcttgatc gtttttaagg tgcgcatata cataaatgac ttcagtttgg
     1681 cgataaaccc tcaatacgaa ttcaatcgca tttaaaaatt gagaagcgcc ttgatcaagt
     1741 gttcctttgt gtttttcaga ttgttttaac tcttctgata attccaaata tttctcatca
     1801 aactcttggt cgcttgaaaa gatcttggtc aagtcccaag ttaaattttc aggcaattct
     1861 tctcgttttg gcaactgctt tacttccatg aaatccactc cttttcttcc cttagctcta
     1921 tctatcaaaa tagatcatag tctaacatcc acatgtcaca gcatctaact tttctactca
     1981 ttcatgtgtc tgcttccatc aattttagca taactaaatc ttgttttaca tagaaaaccg
     2041 attcctagcc tatttttata cttactattt tttcaaagaa gacaagtgta tgcattcgtt
     2101 aaaaaactgc tgatagacca tctcttcatc aatcatagga aaaagccatt cccttttatg
     2161 ttcttttatt tggcaaagcc atgagttgaa atcaggaact ttctggtttt ccaaggcttc
     2221 ttcgtacaac attcgataaa gaaaaacctg ttcttgtaaa taaaagaaaa accgactttt
     2281 ttgatagatc cacgatgata gatgcaaaag atgtttatga cgcaagtagc attgttcttg
     2341 tatggatact gtattctttt gcttgtgaat aagttggcgt gtcagccatt tcttcccatc
     2401 ttcactcact ttttctattt ttttcatttg gttcatttta atttcgttac tagtgaatat
     2461 ttctttaagg tccctactag aaaaagtcca gcagctactg caaaaggaaa tatgccctga
     2521 tacagtctct gtaatatgat gatacaaata aagcttttgt ttcttgagat cagctttcca
     2581 gcaatgcact cctctttttc tattatagta gcagcaactt ttttctatca atgagaattg
     2641 cgattgtcca agaaaagaat taccaagtat ccaccatact tggtaactgt tattcaaata
     2701 attttgggtt ctttcaataa atctctggtg agacagtcta gaacattgga tttctattgc
     2761 tagattcgcc aacaaaatat ccggcctttg ccgcaacctc ggcaaatagg cttccaaaaa
     2821 tactggttct actgactgtc ccagccaatc catgaagcat tcctttaact gtaagtgctc
     2881 ttttgattct ctttcactaa agaccgaaca gttatctgat ttttgatggg caaaatgagc
     2941 acattttttc tttccctttt tcaaaatcac tctttttcga cagccacgac aataatattt
     3001 ctttcctttt tctgcatgtt tagcaaaaca gcacttatta tattcattca ttgcaagcag
     3061 catttttctc cctctgaact tatttacgtc taagaattta tttacacgaa aaaagagggc
     3121 gtgaaaaagc tattctgcat caagtaataa gaacagctaa acaaaaatag ttcccaatat
     3181 ttttcgtttg gttgggcttg ttaccgcaga tgcaagcttt tgaacacatt tatacgaaaa
     3241 aaagactgcg acacaatttt tgtcacagtc tcttactata atgctgattt atacaaaata
     3301 aaatttcgtt cgctcgatag cattacgcgc catcagacat tgtccatatt cattcaatgt
     3361 ttctggtgta cgatcgctct tttcagcaaa ctccaaaatt cgagcaatct gattctctac
     3421 atccgcttgg ttgagatttt ccatccagaa gtgaacgact aggtaatagc gctcttcgaa
     3481 taaatacaaa tcagtccaag cagactctag ttcgacatct gttgcaagtt ggatcacttg
     3541 ttcaaaatct tcaaaagcaa aagttgcttg tctttcctgt tcttctaggg cgcttccaga
     3601 taattcttcc tccggatcgc tgccaataat ctgctgacga agaaagtcac tcacatcttc
     3661 tgaatcaaca gtagaatctt ctgattgaga aatttcttca tccggaagat ttttactgat
     3721 aaacagttct aatccgtctc cttttggcaa aacttggaaa gtgacagctt cgcttcctcg
     3781 gaactcctct tcgatatcaa cttcttccaa aatactataa aagaaatttt cgatttcttt
     3841 gtgattgcca agtaaatcca aaaatgaaac gccccgttcc tctaaatctt catgaccaat
     3901 tagtacacgg atcgtatttt cattaatatg ttccatttcc attttgctac acctcacttt
     3961 gccacaatgt ttaatataga ttcattgtag cgaaatcagc ttaaatgtaa agaaaaaaaa
     4021 tctcgctgtt aagcgaattt aataaaacca gcggattttt caacctttca agctatctta
     4081 tttaactata acatttttgg cagacgaata ctatatagcc gacttttcag ctaattttcc
     4141 ctctctattt ttcgtgcaat catcatcttc tactaccttt ttatacatct aatcaagaat
     4201 actttttgta aaaacaaaaa aacttggaaa aactatcttt gtaatagtct tttccaagtt
     4261 tcgtattatc cttctataat ccagccatca attgtgcttg acgtaattct aattgtcgta
     4321 catcgcgcgg taggaaccga cgaatttcat cttcattaaa accaacttgc agacgtttat
     4381 catcaatcat gattggacgg cgaagcaatc caggattgtt ttgcacaagt tccaataatt
     4441 cttgtaacgg cagatcatct aaatccatgt tcagtttttg gaatactttg gaacgtgtcg
     4501 agatgatttc ttctgttccg tcttctgtca tttgtaaaat cgcttttaat tcagaactgt
     4561 tcaatggttc tgaaaaaata ttacgttcaa caaatggaat ctgatgttct tgcaaccacg
     4621 cacgagcctt tctgcaggaa gtacaacttg gggaagtata taatgttaac aacgtgcacc
     4681 actccttttc gtgataagaa tcactttttt gtaacagaag atcataataa tctcccatct
     4741 gtttctatta tacataatca acttt
//
LOCUS       DACDEG010000132         4761 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.137, whole genome shotgun sequence.
ACCESSION   DACDEG010000132 DACDEG010000000
VERSION     DACDEG010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.137"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            605..1516
                     /locus_tag="IV142_002576"
     CDS             605..1516
                     /locus_tag="IV142_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486164.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            2003..2269
                     /locus_tag="IV142_002577"
     CDS             2003..2269
                     /locus_tag="IV142_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486165.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2341..2682
                     /locus_tag="IV142_002578"
     CDS             2341..2682
                     /locus_tag="IV142_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486166.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2737..3156
                     /gene="ssb"
                     /locus_tag="IV142_002579"
     CDS             2737..3156
                     /gene="ssb"
                     /locus_tag="IV142_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8486167.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            3233..3529
                     /locus_tag="IV142_002580"
     CDS             3233..3529
                     /locus_tag="IV142_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486168.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3544..3975
                     /locus_tag="IV142_002581"
     CDS             3544..3975
                     /locus_tag="IV142_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486169.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            4157..4462
                     /locus_tag="IV142_002582"
     CDS             4157..4462
                     /locus_tag="IV142_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486170.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
BASE COUNT         1759 a          740 c          996 g         1266 t
ORIGIN      
        1 aggcattctc cgacttccag gtttttgacc attttctctc aacgtggaaa aaccgccact
       61 tatgagagaa aatggtcaaa aacctaagcg ggaagtctcc gaataacata ccaagcgccg
      121 acagccaaca gataaagaat acactactaa aaatagtagt agcataaaat ttaaaaggga
      181 aaaaatcaac ccctacccca gtcttggggt ctcaaaacta actcccccct aaccccctct
      241 ttcagcctct tttgaaagag ggggagaatc aaccccacgg ccaacgcccc agtgttcttg
      301 gggctgtcca gaacagaaac ttgcgagaga gtgggggtaa aacaagtgta gaggaaactt
      361 gaagacttgt tcgaacagaa acttgcgaga gagccggagt aaaacaagtg tagaggaaac
      421 ttgaagactt gttcgaacag aaacttgcga gagagttgga gtaaaacaag tgtagaggaa
      481 acttgaagac ttgttcgaac acaaacttac aagagagggg agtaaagcag ttgcagagga
      541 atcaggaaga tttgttcgaa cagaaacttg caagaaagca ttattaaatt gaaggtgatg
      601 aaatatgaag tatttattgc atggctattt tattgattat tctactcgca atagtagtac
      661 tttagcgatt ctatgtttgc ttcatgaatt gagagtggtt actagaaaac agctatacaa
      721 actattgaat tttgacagat caattagtaa acaaggagta acgaatgctt tagtgagatt
      781 atatgaaaaa cagttaatcg atcgaaaaaa atatggaaca gaaatgtatt attatttgac
      841 gaaagaggga catcaaagca taggtgggta ttatacgttg ccgaaagttc ctgaatataa
      901 tttgaatcac catttacaaa ttaacaatta tttgattaaa ggaatggcat tggcaaaaga
      961 ccacccacat ttaaaacgga ttatttcaga aagacgtacc gtctatgaaa cgaaggattt
     1021 aaatcctaag acaaaaggaa gaaaattttt tgtgccagat tttatttttc gttttcgaga
     1081 caaagaaaat catgagatca attggtcctt tgaaattgag ctaacgttga aaacacgtcg
     1141 tcgctaccgt gaagcgattt ttccgaaata tattcgtgag ttgcgaacac gtcctgaatc
     1201 acatttaatt tatgtgacac ctagtggcgc aattcaagaa gaacttcatg cgtttaagcg
     1261 tatttttttc aagcgcgaaa ctgaaaaaga tgcgagtcaa caagaattgt ttgagcggtt
     1321 gcatattttt tcagctttag agtttgaaga gggattaaaa gcattattag agaatgatcc
     1381 agcagtcaat tggcggacag tagaacgaga aaaaatttat ccgaatggcg ctgttcaaag
     1441 tcgtttgaat ttatttgaag aatcaactga aaatgaggaa aaaccaaaag tgaagtttac
     1501 gcctgaagta ttgtaaaaga gggtgttcgc ttcgctcttt ttttgtgcca gtttgctggc
     1561 accgttcaga cgcaagcgtc tgaaccggct cgtttttagg aaggactaac ttgtttgatt
     1621 tatccgttgt caaagaagtg gacgttcgag agtccctaat cgtatagcag tcattcggcg
     1681 agtataacct aaaacataac ctgccaattg ccaagggtaa aatgcacgct gcgcgccctt
     1741 ggcaatcgtc agaacatgtt ttcgagccgg ttatgccgaa cgaccgctat acgaaaaggg
     1801 cctctaaaca gaagtccact ttgatccttg acaactgaaa cccaaaatca aacaagcagg
     1861 ccttcgtcta aaaacgagcc gaatgaagac cttgggggtt gcacccccaa acccccgtgc
     1921 ccccgacaca ctaccgaaaa ggtgggggtg ggtggcgaaa aaatcaaata aaacattcta
     1981 ttcaagaaag gaagaaacaa ccatgacaac taaaaacgag caactgaaag aacaggcaag
     2041 aaaagagttg caagaaagag gtttgattat tgaggggagc tttgaaggag attttgaaac
     2101 ctatattggt tgttatgcaa gaccgataaa caaacctaca gccttagacc caatgaacga
     2161 acaagaagta aaagaacaag aaaaacatgc agtgaatggt tttccgcaag atttcactga
     2221 atggtatgag tgggaaatta acaacgggaa gcttgaaaac ttcttataaa agtcagatga
     2281 cgtgcaaaga gaatgattct ctttgtacgg taaaaatata aagaaagaag gaaaaagaaa
     2341 atggcaaaaa acacacctta tcaagtagga gacatttttt atagtagttg gggctacgaa
     2401 caaacgaacg tgactttttg gcaaattgtg aagttaacag aaaaaacagc atggtttcag
     2461 cctattcaaa agaaaacggt cgagcattat tcgtccatga gtggaaaagt tgttccaatg
     2521 aaagacaagt ttatagagta tcctttagca aaaactaaag acagtattgt aaaaaaacga
     2581 attgatccag aaacctatcc tgatatgtta tgtggttatc gtagttggga cacgtttcat
     2641 agatataaaa atcaagctgt ttatgaaagt agttattatt aaggggatag tttccccttt
     2701 caaaaataat aaatagaaga atggagagaa aaaatcatga tgaattcagt gaatttagta
     2761 ggacggttaa caaaagaagt ggatttaaaa tacacaccaa aaggcaatgc gacaggcaca
     2821 tttattttag ctgttaatcg aaattatacg aatgcaaacg gcgagcgtga agcagattat
     2881 atccgctgtg tcatttggcg gaaagctgcg gaaactttag caaaatatac tggaaaagga
     2941 agtttgattg gcattaatgg acgtcttcaa acgaggagct atcagaatca gcaaggacag
     3001 accatgtatg ttacggaagt tctagtgaca gatttctatt tgttagagag taaagaaatc
     3061 aatgagcaac ggtcaaaatc aatggcgaat aatcaaaata attcttcaat tccaggaaat
     3121 gagattcaaa tttcagatag ttatttgccc ttttaagtga aaaataattt tgttctatac
     3181 ctgatttctt caggtataga acaaaaaata aatagagatg aggcgatttt ttatgaaaat
     3241 cattgacagt tacgatcaac gaaccatgca atatttgggc tcgtttgatt atacagatga
     3301 aaatattatt aattatgttg caagtttacc tccttatcaa agcgttcgct tggtagattt
     3361 tgcaagtgat gaattggtat taacaacgat gggcaacttt ttagattatg tcggagacaa
     3421 aaaatggttg gaaaaaattt tgtctaaatt gattgcgaaa caaacaggag aagaacccat
     3481 aaatgcgatt caattgttca atcgatatga acaagaaaac aatgattaaa gaggagagaa
     3541 aaaatgaaaa gattaaaacg aagtattttt attgtgctag cattgattga ttttgttggg
     3601 acaggttgtg aagcaattga cgttcaaaaa gctcccttat cggaaataaa acaagcaata
     3661 acaaaacaag atgaagaaca accgctaaat acacaagtag aaatagtgaa tcaaaataaa
     3721 ccgagttttt ctgaagaaga tttttcgtta gaaaatggaa ccaggcaatt tttttcagat
     3781 ttggatcgtt taaatcgggt aggtgtagca agtgcacttt tgcataaaag caggatgcca
     3841 acagaagaac gagaccgatt atacattaag ccaactggct ggaaacaaaa gaaaatgaag
     3901 aatggcgatt atctttataa tcgttgtcat ttgattggct atcaacgtgc gacaagtttc
     3961 cccataagta tgtaatacga aacgggagga ttaatcttaa gattaatcac aactaaccta
     4021 tttgacagtt ggaatgaaag ctatcatgtc tgctgaatca actggtctga tactcctaca
     4081 tgcaataaag tctactagga agttattgta tgaagctagg taaaggcggc agaacaattt
     4141 ctaagtgtgg aatcatatga agttggatat gccgggacgc tataaattac tcagggtgag
     4201 actgttttta attaaactga cgaacttgcg aatgtacggg tctataaccg acttatattc
     4261 gaaagatata agattgcgaa tgcaaagaac attatcgaaa agtaagaatg ttggtcatga
     4321 aattcgagat ggaagactag cggtgtatct gtcaagagtt ggtaaaacat gtttgattga
     4381 ttcagatgag agtgggaatg cgacaaaacg ttttacagaa gaaatagagc aactagcaga
     4441 actgaatata cgatattttt aaatacttta gcggagaata aaaaaatcct ctacagaaaa
     4501 ctacggaggg caacatgtgc tattgtttca acaatacatt tttaattttt accataacaa
     4561 aaaagtactt gaaaaaaaac agaaatatgt taaattaaaa atgaaaaaat ggaagcggtt
     4621 acttattctt ttgaatctaa gataaaaatt caaaagaata acataaccca aatatgatta
     4681 tacttgagat tctaaatatt tatcgaaaag gaatttgttt taaggaaaaa tgattctatg
     4741 gacggttatt gatttatatt t
//
LOCUS       DACDEG010000133         4461 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.138, whole genome shotgun sequence.
ACCESSION   DACDEG010000133 DACDEG010000000
VERSION     DACDEG010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.138"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            574..1008
                     /locus_tag="IV142_002583"
     CDS             574..1008
                     /locus_tag="IV142_002583"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486171.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="IV142_002584"
     CDS             2298..3221
                     /locus_tag="IV142_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8486172.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="IV142_002585"
     CDS             3237..3776
                     /locus_tag="IV142_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP8486173.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1668 a          670 c          755 g         1368 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacagagaa cttaaattga attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagaaacg gataaataat aaaaaagact
      181 gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tataaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacaatcca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttta cctagaccaa ttaaaaatca caaagtgagc ccctgtatcg gttgatacaa
      421 gggctacttg gcatactgta cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat taagggcatt ctccgcctcc caggttcttg
     4201 accattttct ctcaacgtgg taaaaatccc acttataaga gaaaatggtc aagaacctgg
     4261 gaggcggaga aaacatacca agcaccacaa gcaaaaaaag ttagagtaac ttccaaaaaa
     4321 gaaaaatttt ttagaacaac ttttgaaaat ttaattttga tttatatccg ttcaatttaa
     4381 acatttgata aactgaaggc tagtatttaa aatgtgtata caagtggtag cgttaaaaaa
     4441 agtggaggta taacctatgt t
//
LOCUS       DACDEG010000134         4430 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.139, whole genome shotgun sequence.
ACCESSION   DACDEG010000134 DACDEG010000000
VERSION     DACDEG010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.139"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            324..2030
                     /locus_tag="IV142_002586"
     CDS             324..2030
                     /locus_tag="IV142_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP8486174.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2188..3090)
                     /locus_tag="IV142_002587"
     CDS             complement(2188..3090)
                     /locus_tag="IV142_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8486175.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(3087..3791)
                     /locus_tag="IV142_002588"
     CDS             complement(3087..3791)
                     /locus_tag="IV142_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP8486176.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            complement(3822..>4430)
                     /locus_tag="IV142_002589"
     CDS             complement(3822..>4430)
                     /locus_tag="IV142_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8486177.1"
                     /translation="NLETITRAPGFFKYTEHTVAAHSYRVASIAQVLGDIEEANGQPI
                     DWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDEFINQEIPAEFQ
                     EVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKEMFIEAVTTIKS
                     FEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
BASE COUNT         1348 a          937 c          784 g         1361 t
ORIGIN      
        1 ccggagtttc ttgatgaaaa tatgcttcta tcattacaag ctcgtttggt gtaagatggg
       61 tataagtcat ttatgttcac tctccttgta tgctttagcg ggtattacaa tttgagtgta
      121 acataaatgg cttttttatt tgtctcgctt aattatacaa acggcgatac ctaaaaattt
      181 ggatttcaac gatagaaaca ctgtttttta tgtaattagt ccaagatttt tctagacttt
      241 tgctttacta ttcggccaaa ttggtttttc ttcttttttt cgttataatg aatatactta
      301 tgaaaagaaa tgaggaatga accatgtcct ttgatggtgt atttacccac gcgatggtca
      361 acgaattgcg tgaaacgtta ctatctggcc ggatctcgaa aatccatcag ccttatgaaa
      421 atgaaatcgt gctggtgatt cgttcacgcg gcaaaaacca tcgattatta ttatctgcgc
      481 acccaagtta tgctcgtgta cagattaccc aaatcgatta tcagaatcct gataatccgc
      541 ctaacttcgt gatgatgctt cgaaaatatt tggatggcgc tattttagaa gacatcgaac
      601 aaatcgaaaa tgatcgagtg atccatttcc actttgctaa acgaaacgag ttgggtgatc
      661 tacaaaatat catattgatc gttgaattaa tgggacgtca tagtacgatc gtcttagtta
      721 atcgagaaac tggaaaaata ctggatgcga tcaaacacat tggaagctct caaaacactt
      781 atcgttcttt gcttcctggt gtagaatatg ttgctccgcc aaaacaagag gtcttgaacc
      841 catttagctc cgagaaagaa aaaatctttc agcggctttc ccaaactgaa ctagatccaa
      901 aagcaatcca acgtcagttt cagggaatcg gtttcgatat cgctcaagag ctgaccaaaa
      961 gattactgga acgtcctaat gaaaagatgg ttgtatggga cgaatttttc tcagccatca
     1021 gccatcagcc tattcctact ttttacgaaa cggaaaataa agattttttc acaccgattg
     1081 cttatcaggt attgtccgaa caggcatctg ctgtaacagc ttatccaaca ctcagtcaat
     1141 tattagatag ctattatcac gaaaaagcag agaaagatcg ggcaaaacaa caaggcggag
     1201 aactgattcg aaaaatcgaa aatgaactaa aacgaaataa aaataaattg aaaaaacgtg
     1261 aacaaacact caaagaatcc gaaaacgctg agaattaccg tagaaatggc gaactactta
     1321 ctacttttct gacacaagta ccacgcggcg caaaagaagt tgttttacct aattattatg
     1381 aagaagatcg gccaatcaaa atagcactag atccggcatt gacaccaaat caaaatgcac
     1441 agaaatattt ccatcgatac caaaagctga agaacgctgt taagctgatt ggcgaacaaa
     1501 tccaagaagc caaagatgaa atccaatatc ttgaatctgt cttatctcaa ctggaaattg
     1561 ccggcccaat ggatatcgaa gcaattaaag aagaattgac ttcagaagga tacctgaaaa
     1621 agaagaactt aaaaaaacaa aaacgtaaga aaccatctca gcctgatcaa tacttttctt
     1681 ctgacggaac gctcattctt gttggtaaaa acaatcttca aaatgaccag ctgacaatga
     1741 agacagctaa aaaaaccgac tattggcttc atgcaaaaaa tatacccggt tcgcacgtca
     1801 tcattaaaag tgacaagccc tcagatgaaa caattacaga agctgccgag ttagctgcct
     1861 atttttcaaa ataccgatac tctgcccaag tgccggttga tcttgtacag gtaaagcata
     1921 tccgaaaacc aaatggcgcc aagcctggtt atgttattta tgaaaaccaa aagacgatta
     1981 tcgtgacacc agaagaagaa aaaattacag ctatgaaaca aaacaggtaa tcaatgagtg
     2041 tttctagaat catatttgcg tactttccca caacagcaat tagttataaa ttgtttgcta
     2101 caaaaagagg agttgtgaca aaattctgtc acaacccctc ttttttgatc aacggtgttc
     2161 aataaacagc gttctcatct gcttttctta gctctgctgg tccagtaacc gagtaagctc
     2221 tttgataaat ctttgagcga tttcactgag cggacgtttc gccgaataaa tgatataaaa
     2281 ttcatttagt tcgccttctg ccaaagggat taaccgcaag tgctcgcgaa tccctccaat
     2341 cacaatacca gatcctgaag cgtaagcatc agtttcaaga acgatattca tcaaagtacc
     2401 tcgatcactc gtataaatca ctgtttcttg atcagggatc tcgataaggt cttcataaaa
     2461 atacgggaaa ttatttccat cctgtgtaaa acgaacttga ggataagata acaattcttc
     2521 tttggtgatc tctgcgtgtt cagctaatgg atgtccttgc cttaaaaaaa tatgcgtttt
     2581 gaattttcca atgctgcggc cacttagctc tccttgttgt aaatatcttc tgataccagc
     2641 ttgattatgc tcattcatat aaataatgcc gatctcactg tgctgctctt tgacttcttc
     2701 gatcactctc gctgttgtcg tctccatgat ccgaaattgt ttgatctcgt ctttgaattc
     2761 ctcgagtagc tgtgcggtca aaggacctaa gaaatcataa tgctgggcag cgatcgaaaa
     2821 attgtctctc ttttcactcg ttagatagtg agtttctaat gtatcaagct ggaccaatat
     2881 tttctcggca cgatttaaga agtcttgccc ttcttctgtt aaatatgccc ctcgattcgt
     2941 tcgagtaaac agcgtgatcc ctaactcggt ttctaattct ttgataccag ctgaaagact
     3001 tggctgggag acaaacattt tctttgccgc ttctctgaaa ctaccattgt ttgctacagc
     3061 gaccacgtat ttcatctgct gaatattcat tgctcaaact cctcgaaata ataaatttct
     3121 cttccattca agggctggat tggtcggttg ttcggtcgag catactgacc atcgaagggg
     3181 agaagaaact cttggttcag ttttcctaca tgatccatta caaaccattg gattggcccg
     3241 ctggtaggcg gagttgtcaa tgagccaata taatggaaat ggcttttttg ctccggaaag
     3301 aacacatccg gatttaccca atgctcatgt ttttctaagt tccaactttc cccattttct
     3361 tgattataca gccaaccttc ctgtaccatt agctggtaca tgacacccac aactagattc
     3421 tcttttttgc tgttcatatg aacaaaatga aattcaatat cttcctgttt tccgttgatg
     3481 atatgttcag aaggcaagtg gaaatgaata tctgtcaaat aatagcgttc tttcttgaac
     3541 tctacataac tgagttggtc aaaaggtact aaatgaatcg tgtttttgaa ttccttgtca
     3601 gtaaaccgtt gcttttgata atgaaaggaa agcgtgtctc cttctatttt ggtttcttcc
     3661 cccttggtca aatgaatagg cgactgcaat ggaaattccg cacctctcgc aaaccaatca
     3721 cataaactcg cccaattttc cggacctttg tctccagaat agccccattc gacatccata
     3781 ttctttttca actatacttc cccctgcatc tcatatcatt tttatttttc ttctttcttt
     3841 acaatcgttg agacggtttc taaaaacgcc tcttttccat aaaattcctg gttcaatagt
     3901 tccggaaata tctctttaaa gaaatactga acagaaggcc gatgttcaaa gcttttaatc
     3961 gtagtcactg cctcaatgaa catctcctta taaacttttt ccgggttatt tttgatgatt
     4021 tcatcaaaag attcataaag aaggtcaacc ttatctgaaa tggctaatag ttctccttct
     4081 acggtttcat cttttccttc gaacaaccga cggcgataaa cttcttggaa ctcagctgga
     4141 atctcttggt tgatgaattc gtccgtcatc ttttcttcaa ctgtttgcag catccctctt
     4201 aactctttgc ttgcgtattt caccggcgtt ttgatatcac caataaaacg ctctgtgtag
     4261 tcatgattca atgatttttc atagagcagt ttccaatcga tcggttgtcc attcgcttct
     4321 tcaatgtctc ctagaacttg cgcaatagat gctacacgat acgaatgtgc tgcaactgta
     4381 tgctctgtat atttaaaaaa tccaggtgcc cttgtgatcg tctctaaatt
//
LOCUS       DACDEG010000135         4397 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.140, whole genome shotgun sequence.
ACCESSION   DACDEG010000135 DACDEG010000000
VERSION     DACDEG010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.140"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            266..967
                     /locus_tag="IV142_002590"
     CDS             266..967
                     /locus_tag="IV142_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8486178.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1539..2555)
                     /gene="tsaD"
                     /locus_tag="IV142_002591"
     CDS             complement(1539..2555)
                     /gene="tsaD"
                     /locus_tag="IV142_002591"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP8486179.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2552..3016)
                     /gene="rimI"
                     /locus_tag="IV142_002592"
     CDS             complement(2552..3016)
                     /gene="rimI"
                     /locus_tag="IV142_002592"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP8486180.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(3023..3565)
                     /gene="rimI"
                     /locus_tag="IV142_002593"
     CDS             complement(3023..3565)
                     /gene="rimI"
                     /locus_tag="IV142_002593"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP8486181.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3549..4373)
                     /gene="tsaB"
                     /locus_tag="IV142_002594"
     CDS             complement(3549..4373)
                     /gene="tsaB"
                     /locus_tag="IV142_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP8486182.1"
                     /translation="MYHCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDKIIVPMFDARRKNVYAGAY
                     RWKENRLEIVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFYEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
BASE COUNT         1320 a          910 c          747 g         1420 t
ORIGIN      
        1 cattgaaaaa aattttttga ataaattaaa aaaataaatt atattagcgt tgttagcgtt
       61 tactatatgt attattgttg ttttttatac acaaatcatt cgcttcaaaa caaattattc
      121 aataccattg tatatttttt acataataac aaattatatt tgtaaagtat taaaacttgt
      181 ttctaatgtt ggtatgttaa aactttacaa attctttaaa aaaagtgaca ataaatatag
      241 tgaacaataa ggagaggact ttttaatgcg tgttaataaa atcgattttc acttttcaaa
      301 actcagatat caacctgatt ttgaacattt ctcggatgaa gagttccaac agatcaagga
      361 acatactgtt cttcgttctt ataaaaaagg acagatcctt ttcgacgatg gtgatcccag
      421 acaacggttg tatatcttga ccaaaggact cgttcgttta gaacgttatg atgaaagtgc
      481 aacttattac tactatgact atgtgaatac aaataacctt tttcctatgt caggaatgtt
      541 tggagaagag acctacactt atacagctca agcgatgact gatattgaaa cattctacct
      601 tcctgtttcc tactatgaaa aattagcacg aaaaaatccc gaacagttga tctatatgat
      661 acaaaaaatt tctaaagtag ttgaaataca tgaattgcgt atccaaatcg gattgacctc
      721 tagcgccttt gaccgggtaa aacaaaattt attcattttg aaagaagaat tgggcgtacc
      781 aacaaaagat ggtcatatca ccattcctta tccgatcacc ttaaaagaat tagcagtgaa
      841 tagcggcacg acacgtgaaa ctgctggaca agtaatcaaa caactacgtg attccggact
      901 gttagattat gacaaaaagc gttttacttt ctgtaagaca gctactactc cagctatgag
      961 agaataacgc gaacaaaaat aagcagtgac cctgatattt atcagggtca ctgcttattt
     1021 tcctccctaa aacttctgaa agatccggca gttttagatc actgggcaag actcaaagat
     1081 tttctatgtg tgtttcagtt atgtaatatt cactgtttgc aaaaaatact gatagattaa
     1141 ctagaggaag cgtcttgtcc tcatccgacc tagattattc gatattcctc gacaataaac
     1201 gattaatcag tgagggcaat cagaggaacg gataatgatg aataagctgt aaaaaatagg
     1261 gctttgacaa aagcgtgtca cagcccttat ttcgattaaa cggtgtgtac ctgtcaactc
     1321 ttcggtcaca agtcataatg ttcataaaca tgaggagctg ttttttgaaa ttgttccttg
     1381 tgaatcagag ttaaacgaca ggcttacgaa ctcttgatta aacggcgttc aaaagcttgc
     1441 atacgcggta ataagaacag ccaggtcaaa aacatgagaa gccgtttttc ctgggctgtt
     1501 cttattactt gatgcagggc agatttttca caacctcttt attcagataa aaccaagctt
     1561 ggatcagcat tcaactgata gctagcaaaa tttctttttt gcatttccac atgtgctgct
     1621 gctccaatca ttgcagcatt atctccacaa agtcgaagag ggggaatcac tagttctacc
     1681 tcagggagtt tagttgccaa agcatcctgc aatccttcac gcaacccttg attggccgct
     1741 acacctccag ctacaatcag ttgcttgact ggataatttt gacaagctcg aagtgtttta
     1801 gtaatcaata catcgatcac acttgcttga aagctagctg ctaaatcatt tttgtctaga
     1861 tcttctccac gctgttgtgc attatgcacc aaattgataa atgcactttt taatccgcta
     1921 aaactgaaat cataattatc ttcatggatc atcgctcgtg gaaaatggta attatctttt
     1981 ccctgatgag ctagctgatc gatttctttt ccgcttgggt aagaaagacc taatactctt
     2041 ccaactttgt cataagcttc tcctgctgcg tcatctcttg tctccccaat gatttcatat
     2101 gatccatctt cttgcatata gactaattct gtatgtcctc cgctaacaag aagtgccatc
     2161 aatggaaact ggaatggttt aaccagacgg gctgcataga tatgccctgc catatggttt
     2221 acaggaataa gcggcaattg gtgtgcccaa gcaaaagcct tagctgcaga aataccaatc
     2281 agcaacgagc cgacaagtcc tggtccataa gtgacagcga ctgccgataa atcttctgcc
     2341 gacacaccag cttctaccag tgcatcttcc aagcataagg tgatttgttc tacgtgatgg
     2401 cggctagcta cttcaggaac tacgccacca aaacgtttat gactgtttat ttgagaagca
     2461 acgatgttag ataagatctc tgttccattt ctaacgaccg ctacactcgt ttcatcacaa
     2521 cttgattcaa tcgccagaat caattcttca ttcatttcat cgtctccgtt tttaactttc
     2581 tgctcataat gatcgcatct tccgtcggat tgcgataata tgatttccgt ttacctaata
     2641 tacgaaactt ttctgattca taaagttttc ttgctgcttc attagataga cgtacttcta
     2701 aaaaaacatg cagcgaatct tttatttgtt ccgtaatcag taattgtaac aaatgccgtg
     2761 catgcccttt tccttgttct gaaactgcaa cagcaatatt tgtgatctcg acttcatcta
     2821 ttactttcga gtatccaata aagccacaga tctggttatc ttcaactaaa agcagataat
     2881 cagagtgagg ctgttggatg tctgccaaaa actgcatttt cgtccaaggt gaaccatgtg
     2941 gataagcatt ttcgctaatc atccatacgc attctgccaa gtacgcttca tcaaaatctt
     3001 tttttctgca aatcaactaa tctcactcat tttctaaaag ttttatcata tccaacgcat
     3061 cttcttttgt ttcagtgtaa tatctttttt tgatcgttgt tgcttgaaaa cctaacttcc
     3121 gataaagtct ttgtgcatct tgattactca tacgcacttc taatgacatc gtctcacagc
     3181 gattcattat agcaaagttc tcgatttcgt caataagcag cgaaccgatt cgtttacgct
     3241 gatatgccgg tcgaacggca atattcgtga tatggcaatc taaaccgaac gctcttgaac
     3301 cgataaaacc aatcattctt ccattatcaa agatacctag atatagatga ggaagcggtg
     3361 acttcaattc tgatataaag gcactttttg tccatggcaa ttcgccatta tagacttctc
     3421 gttcgacatc cagcagctct ttgatatctt ctgtagtcaa ttctcttagt acgtaacgac
     3481 gatcgatttg ttcgattgtt ttttctgtat aaatcatttt atcttgaaaa aagcttctgg
     3541 catacgctct aaattttttc aacataactt tcgtctccag gtgtgtgcgt ctccaaccat
     3601 ttttcttctg cttctacccg ttttaaatag cgaggaagaa aagcttgttt ctctgcaact
     3661 ggttcagcta aagcacctaa ttgagccaca gttacaccac ttggaatatc ccatgctgga
     3721 actgtattaa tcatcgcact aggcaatatt tcctttattt catagaagaa tttgtgacaa
     3781 tcactcccga cgaaataaat agcttcattc agcttttgta gttgtgtgaa caaatcagaa
     3841 gcagaaatat gtacatcttc aagtacaatt tctaatctat tttctttcca gcggtatgcc
     3901 ccagcgtata cattttttct tcgagcatca aacatgggca caataatttt atccacaccg
     3961 atgcaattag ccgcgatggt tttcagacta gatactccta ctagctcttt atctaatgta
     4021 taagcaatcg tttttgctgt cgttacacct atacgaagcc ctgtataaga accgggacca
     4081 tcagaaaccg cgattcggtc aatgtctttt ggacttattt gaagatctgc aaacaattgg
     4141 tcgattgcag gcatcagtgt tacactgtgg ttgcgtttga tatttgtttg tatttgtcca
     4201 agaatttgtt cgtcttctac cacaccgatc gccaaagttt ggtttgcagt atctattcct
     4261 aatgtaatca tatatttgat cctttctgct atgacattct cgccttccat tgtagcatac
     4321 ttttttattc tgaggatgaa aataccaaag gcaaaactag tacaatgata catgattatc
     4381 tgctggcggg caaattt
//
LOCUS       DACDEG010000136         4396 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.141, whole genome shotgun sequence.
ACCESSION   DACDEG010000136 DACDEG010000000
VERSION     DACDEG010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.141"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            118..1005
                     /gene="xerD"
                     /locus_tag="IV142_002595"
     CDS             118..1005
                     /gene="xerD"
                     /locus_tag="IV142_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP8486183.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            1024..1395
                     /locus_tag="IV142_002596"
     CDS             1024..1395
                     /locus_tag="IV142_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP8486184.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            1365..2162
                     /locus_tag="IV142_002597"
     CDS             1365..2162
                     /locus_tag="IV142_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP8486185.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            2146..2715
                     /gene="scpB"
                     /locus_tag="IV142_002598"
     CDS             2146..2715
                     /gene="scpB"
                     /locus_tag="IV142_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP8486186.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            2721..3437
                     /locus_tag="IV142_002599"
     CDS             2721..3437
                     /locus_tag="IV142_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP8486187.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     regulatory      3519..3644
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            3767..4378
                     /locus_tag="IV142_002600"
     CDS             3767..4378
                     /locus_tag="IV142_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8486188.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
BASE COUNT         1474 a          772 c          965 g         1185 t
ORIGIN      
        1 aaaaagaaac cagtccgtct gagtgtctag acggcttttt ctttgctcag attatggaaa
       61 aagactatca gaatgaagcg gtatctgata tgatagagag gcatgagggg gattattatg
      121 aaagagcaaa tcaacgatta tctccactac ttgaatattg aacggggatt gtccgccaat
      181 acacgacaaa gctatgaacg tgatttggaa cagtacttgc attttctaca ggagcagaaa
      241 attgcttcat ggcaggatgt cgatcgcttt ttagtcatcc gttttctgga gaagttgcat
      301 gaagaaaaaa aagcgacagc aactgttact cggatgatct caagtctcag aagattccat
      361 cagtttttac ggcaagagcg tatcactgat catgatccga tgcaacatat cgatacgccg
      421 aagaaagcac agaaattacc aagtacattg actctgacag aagtagaaaa gctgattgaa
      481 gcacctgata cgacaaaaac gttagggata agagatcgtg caatcttgga agtcatgtat
      541 gctactggga tgcgcgtcag tgaattgatt ggattgaaat tgggagatct tcatctttct
      601 cttggactgg tacagacagt tggaaaagga gataaagagc ggatcattcc tttaggtgat
      661 tatgcgatcc aatggctgga acgctactta gacgaagcac gtccattact tgtaaaggac
      721 gcatctgaaa tgcatgtatt tgtcaataat catgggaaag gtctttctcg gcaaggaatc
      781 tggaaaaatc tgaagcagtt agtacgagag gctgggattt acaaagaagt aacaccacat
      841 acgctgcggc atagctttgc cacacatctg ctagaaaatg gagctgattt gcggacagta
      901 caggaattgt taggtcacgc ggatatctct acgactcaga tttatacaca tatcacgaag
      961 aaaagaatga cggatgttta taagcagcat tttccaaggg cttagtcata ggaaggtgag
     1021 aagatgttaa cacattaccg gaaagaaaac cgtaaaattg ctatgggact attgagtttc
     1081 cataagaaac tgacaggtaa aacttctttg ttgaaggaga ttgatacgta cgaatctgaa
     1141 gacgattatg aacttttttt ctttacgcca gaaggttcaa ccaacattca agggattatt
     1201 ggtatttact gggcaacgaa cgatcagctg atcatccatg acatttcttt gaatccgtct
     1261 tatcgcggtg agaatatggg ctttcaaatg ttagatgaat tacaggaaaa gtacccggat
     1321 gtctccatcc gtgcaactga aatcacagag ccttacattg gaaaatggct agcaaagaaa
     1381 aagaagaaag agtgaatgag ttgacggaga tcaatattaa attagatgta ttcgagggtc
     1441 ctctcgatct gctgcttcat ctgattcagc aaatggaaat cgatatatat gatataccga
     1501 tcgctacgat cacggagcaa tatatgaatt atatccatac aatgaaaaca ctcgagctag
     1561 ctgttgcagg agaatactta gtgatggctg ctacattgat ggcaatcaaa agcaagacat
     1621 tgcttcctgt ccaagaattc ggcacagacg atgaagaatt atgggaagaa gatcctcgag
     1681 aagaattggt cactcagctt ttagaatacc gtaaatataa atatgcagct gaaagactga
     1741 cagaaaaagc agaggaacga agtctttatt acacaaaaga acctatgaac atcgatgggt
     1801 tagcggatgc tgaaaagccg ttgaagcgag gacaagtcaa aaaaatcgat ttgatccgtg
     1861 ccatgcagaa aatcttagaa cgtaagaaat tttctcaaaa agtggaaaaa acgatcacgc
     1921 ccgatgacac aacgatcgaa gaacgaatgg tatttatcga agagcgtata aaaagaaatg
     1981 cgcatggctg tactttcgat tctttttttg agtcaccttc taaaagtgaa gttgtcacta
     2041 cttttatggc tttgctagaa ttgatgaaaa aagggcagat cattgcgcaa caaaaagaaa
     2101 actatgaatc aattatgctt tatccagcag taggagatac cgaacatgac gaaattaagt
     2161 gaacttgaag cccttctttt tattgttggc gaggaaggga tcagtaatga agaattaagc
     2221 ggactgttaa atctcagcga agcagaagtc acaagtttag tgaaagaatt aaaagaatct
     2281 tacactcaaa actcgcactc agctttgcat atttttgaaa cggaaagcca ttatgtatta
     2341 acgactaaaa aagagctggc tcccttactt aagcgatatg cgcaagggac atcaaacaca
     2401 ttatctcaag ccgccgtcga aaccttagca atcatcgctt ataaacagcc aatcacaaga
     2461 gctgaaatcg agcagattcg aggagttcag gtttcaggag cggtacagcg tctagctgcc
     2521 cataaactga ttgaagaaaa aggtagagta gaaggaccag gtcggccgat attatatgga
     2581 acaactggct acttcttaga ttattttggt ttgaaatcat tagaagaact gcctgataca
     2641 caagaaatgg aaatttcact tgaagaagac gtgtcaccag atctattctt tgatggaatg
     2701 aataaaggag aataattgta atggagagat tgcagaaagt aattgcccac gctggcatca
     2761 cttctcgtcg aaaagcagaa gaattcatcc ttgcaggacg tgtaaaagta aacggacaag
     2821 ttgtaaggga attagggata aaagttggga aacatgacac tgtggaagtg gatgaagtcc
     2881 caatttatca agaagaatac ggttactatt tattgtacaa acctaaagga gttatttctg
     2941 ctgttgcaga tgacaaagga agaaaagttg ttacggatct tttgccgatg gtaaaagaac
     3001 gtatctatcc tgtaggtcga ctggattacg atacttcggg gatcttgcta ctgacaaatg
     3061 atggagattt tgctcagcgc ctgacacatc caaaacatga agtggataaa gtttacgttg
     3121 caaaggtaaa agggatcgct acaaaatcga tgcttgcacc tttgacaaaa ggaataaaga
     3181 tcgaaggacg aaaaacatct ccagctaaat tccaaatttt atcagtggat cctaagacaa
     3241 accacagtat cgtggaattg acgatccacg aaggacgtaa tcatcaagtg aaaaatatgc
     3301 ttcaagcagt tggtcttcct gtgcaaaaac taaaacgtga aaaatacgga gatctgactt
     3361 tacaaggcct acgtcctggg gattaccgtg aactgaacaa aaaagaagtc agcagtttgc
     3421 tgaaccactc aaaatgaaca cttacttttt ataattgtta gatgttgcgt tttttgaata
     3481 gatatgtata atgaatatgt aaacaaaaat tatataaggt tcgtcttcag gggcagggtg
     3541 aaattcccga ccggtggtta tagtccacga cctattttgc aaaaaaatag ctgaatcggt
     3601 gaaattccga taccgacagt atagtctgga taaagaagat agagcttatt tgacgacgct
     3661 tgatcaaata actctaactc tattttccct ggaaaataga gttttttcat ttggagagaa
     3721 acgtcaaaaa gttcgctgaa gaggagtcct aacaggagga tttcaaatga agaacacccg
     3781 tgtacagaaa atggttgcag tagcgatgtt tgctgcgatc gcattagttt tacagtattt
     3841 tgcttttccg atcatgccgg cttttagttt tttgaagatc gattttagtg atatctcggt
     3901 attgatcagc atgttcttat tcggtccagc ggccggagtt gcaacagcgt tcttgcgttc
     3961 agtattacat ctgctgacta caggtttttc tccggataat ttggtaggag acactgctag
     4021 tttcttatca acaacgatct ttacattccc gatttattat ttctttagtg taaagaaaca
     4081 gcataaagga agaaacaaat ttttaggtgt cattacagga accatcgctc taactatttt
     4141 tatgagtatc gcaaattatt tcgttattac gcctctttac ttaatgtttt tagggctgaa
     4201 cgcaacacag atgctgggca tgtctttagc aaactatgta ctgattggga tcgttccttt
     4261 caacttgatc aaaggattca tcgtgagtgc tgtcttttta gtgctgcatg cgcaacttct
     4321 tccatggtta agtcgaaaac aacatcagtt cgaacaacgt catacagcag tcaaataaat
     4381 atcaaaaaga tcaaga
//
LOCUS       DACDEG010000137         4291 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.142, whole genome shotgun sequence.
ACCESSION   DACDEG010000137 DACDEG010000000
VERSION     DACDEG010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.142"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            191..532
                     /locus_tag="IV142_002601"
     CDS             191..532
                     /locus_tag="IV142_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP8486189.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYYQKLTDNPIGFGLLPEDDEGNEWFKMTLTNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            646..885
                     /locus_tag="IV142_002602"
     CDS             646..885
                     /locus_tag="IV142_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486190.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            997..1191
                     /locus_tag="IV142_002603"
     CDS             997..1191
                     /locus_tag="IV142_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486191.1"
                     /translation="MEEKLLKTMKQKHLKRLSVMQYINDMQITGKEKACLLGSMKNFE
                     QLRRTYVKTSSNCQLLLEVS"
     gene            1277..1948
                     /locus_tag="IV142_002604"
     CDS             1277..1948
                     /locus_tag="IV142_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP8486192.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQQEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            2001..3977
                     /locus_tag="IV142_002605"
     CDS             2001..3977
                     /locus_tag="IV142_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8486193.1"
                     /translation="MAINRYVKKAGMLIGMLGIIAGCFQGYRAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            3990..>4291
                     /locus_tag="IV142_002606"
     CDS             3990..>4291
                     /locus_tag="IV142_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8486194.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTILSISLKLPIYDGTSDKILENGVG"
BASE COUNT         1570 a          635 c          931 g         1155 t
ORIGIN      
        1 tcattggatc aatcgagcat ttaccgattt ctactagggt ttctcgtttc ggacgccata
       61 aacgagtcga tattttttat gtatttttaa gtaaacgaaa aacgataaaa tatgaacgag
      121 tcctgaataa agtgaaaaaa ttgatataaa acatagagaa aatgaaattt acgtaatgga
      181 ggaatgacaa atgaaaaatt atgatccgaa tatacgattg ggcacacata ctattaaggt
      241 ttctttccaa cgttgggatt acaaaggatt tgtgacgttt agaagaggcg gaaattgtaa
      301 aggattagat gttttagcat tagacgagga tgatttatac tatcaaaaat tgactgataa
      361 tccaattgga ttcggcttgc tacctgaaga tgatgaaggt aatgaatggt ttaaaatgac
      421 gcttacgaat gataatggag acgaattatc tgttgaagat acttggtcat atttgagtga
      481 ttatattgtc agcgttgaaa tcattgaatt tgtagcggat aaagaagaat aaaaactgga
      541 ggaataaatt ttacagataa aggcttagaa gaagtcacga cgaataataa aaaggtagta
      601 ttaccttagg cattgagtaa ggaattcatt taggaggaaa attagatggc gcgaccagtt
      661 ttagaggcga tggatatgga tactttgaca aaaggaaggt atgtacagct gttagctcgt
      721 ggttattcac ctaaagatgt attcaaatcc ctgtctaaag gaacagatat ggaaaaagaa
      781 agattacgaa aagaattcga ttattggaga acccataatg gaatcaagga tttgaaacca
      841 gcacggccag taattaaacg aaagaaaaag ataacccaag actgaagtgt agagcgtatg
      901 tgatcatagg cttttttatt tttgcaaaaa acaaggaagg taggaaaact catgaattat
      961 gaagaaatcg aaaacagaaa aaaagtgtcg aaataaatgg aagaaaagtt gctaaaaacg
     1021 atgaaacaaa aacaccttaa aagattatct gtcatgcaat atatcaatga tatgcagata
     1081 acaggaaaag agaaagcttg tttgttaggt tcaatgaaaa actttgaaca gttgagacga
     1141 acttatgtga aaacaagttc taattgtcaa ctattgttag aagtctcatg acattcatta
     1201 atagaattca tttttaaaac tttttctatg gaaatgagaa aaagattttt tgttttgtct
     1261 gaaagggggt agctggatga gaaaatggtt atttggtttt ttaggagtcg ctttaatcgt
     1321 cttttgcagt gtgtttggct atgttagcta ccaaaaacat gaaggagagg tgtttaagca
     1381 aaatatagaa aaaaagatgc ccgtagatca aatcaatgca catgcaaaga gttataagga
     1441 ggatgcaacg aacgtcaata atgatatgtc tttggggcaa atgttgtcca tacaacaaga
     1501 agcaatcgaa atgggcgtca ataaacaagt atttgcacaa attcagattc ctgcacttgg
     1561 tttagcattg ccaattttta aaggggcaaa tcaatacact ttgagtcttg gagcggctac
     1621 ttatttttat gaagatgctg aaatgggcaa aggaaactat gtcctagcag gtcataacat
     1681 ggaaatgcca ggtgttttat tttctgatat tcaaaagttg tcattagggg aggtgatgga
     1741 cttagttagt aacgatggcg tctatcgtta caaggtaacg cgtaaattta tcgtacctga
     1801 gtactttaaa ttaatcgatg gcgttcctga agagaactct ttcttgtctt tgccaaagaa
     1861 aggagagaaa cccttattaa cgttgtttac ttgtgtttat acgtcacaag gaaaagaaag
     1921 atacgtggta cagggagaac tacaataaca aggttttaat caacgtgagt cgttgaaata
     1981 tgaaatgagg agagtaaaaa atggcaataa atcggtatgt aaaaaaagct ggaatgctta
     2041 ttggaatgtt aggaatcatt gcaggctgct ttcagggata tcgagcatat gcagaagatg
     2101 tcacgcaaaa aacgccacca gagaaggtca atatcacggt tcataaatta atgtacgatc
     2161 aaggaacgca attgaatgtt gatattgacg ggatcaaaaa cgatggttat acgcacgatc
     2221 aatatcctac aggtgtaacg aaatacaaca aggcagatta tggtgatgtt gagtttacgt
     2281 taggaaatat tacggatcaa gtattaccga ctgaagattc tgatctaacc aacgcgaagg
     2341 tggacgaaat cgttaaagat gtagaagaca agggatctaa cagtgagtac gtcaagaatg
     2401 caaccaacaa aattacttca gctgtagatg aaaatggtga gatcactttt gctgatcaac
     2461 cagcctatgt caattccaaa gggaatgtat atgtggttta tgaaagtaaa tccgctgctg
     2521 gattagtcac acaaaaagca aaaccaatgg ttgtgattgc gccaatgact gataatacag
     2581 gttcaggttt tttaaaagac attcacttat atcctaaaaa tattgtttct aagttgagtt
     2641 ttgaattaac gaaatttggt gacgatggca cagctcaaag caaacaaaca cctttaaaag
     2701 gggcaaaatt tgagttatac aagggcgaac ctggaaaagg aacaaaatta ggagatcttg
     2761 tatcggatga tcaaggaaag ctgactgcta ctgatttgac gttaggaaaa tactattttg
     2821 tggaagttcc ttcagaagtt gtagtgggat cagataaaga accaacagcc gatcaatatt
     2881 tattaggcgc agacgctcgc aacgatgcgc acaataaatt aacctttgaa attacgaatg
     2941 atggcgtgac aagtgactta aaagcttcat acgtaaacta taaagcacca gtgatcgata
     3001 aaacagtaac aaatggaaca ggacaagaac attctttcca aattggtgac gcagtaaatt
     3061 atcaaggaac gattcatatc ccaacagaca ttgcaggcgg agcagacggt atcacagtaa
     3121 acggagtgaa aagcgaaact tctccttaca gtgtatttaa atggggggat acagcaggac
     3181 aaggactttc ttatgtcgct gctaaagcaa atatcaaagt aacaaataaa gatggatctg
     3241 tggtattaaa agaaaataca gattataaaa tccaaaattc agaaaatggc tttgtgatcg
     3301 attttatcgt gaataatggc caagtaagtg atactgtagc aagtttacac ggacaagatc
     3361 ttcaaatgac ttataacatg tatgtaaatg acagcgcagc tgttgccaat ccattaacaa
     3421 atagcgtgga tttcgtttat aacaacaatc catttaatca ggaagaacac cacgaaaaaa
     3481 cgaaagctga cgttgtgact tatggtgcaa aattcttaaa agtcgatagc ggactatttg
     3541 gaacaggaat caaagcaaca ccgcttgaag gcgcagaatt tgctgctaaa aacgcagaag
     3601 gaaaatatta tggtggatta gttgatacgg ataaagacgg tgtcaaagaa gcagtatggg
     3661 tagacgatgt agcaaatgca gcgatcctaa aatcagataa agaaggtcac tttgaaatca
     3721 ctggtttaac tgaaggtgaa tattcactgg aagaaacaaa agcgcctgaa aactatcaaa
     3781 aattaacgaa agagatctct ttcaaagtag acaaagattc atacaaagaa gaaaaccgaa
     3841 tcacgatcaa aaacaatcaa aaagcttccg taccaatgac cggtagcaac ggtttccaat
     3901 cttacgtatt gatctcttgc ttgttgcttg gggcaggcgc aatttctgca gttgtttact
     3961 ttaagaagaa agcctagaag gatcagctaa tgattatcag acaccctaag aaaaagcgaa
     4021 tcatgggtaa atggatcatt gctttctggt tactttcagc tgttggtgtc ttacttttga
     4081 tgcctgctga agcgagcgtg gcgaaatacc aacaaaatca gcagattgcc gcaatcgatc
     4141 gaacaggaac ggcagcagaa acggattctt ctttggatgt tgctaagatt gagttaggcg
     4201 atcctgttgg tattcttaca attctaagta tttctttaaa actaccaatt tatgacggaa
     4261 cgtccgacaa aattttagaa aatggtgtag g
//
LOCUS       DACDEG010000138         4222 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.143, whole genome shotgun sequence.
ACCESSION   DACDEG010000138 DACDEG010000000
VERSION     DACDEG010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.143"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..345
                     /locus_tag="IV142_002607"
     CDS             <1..345
                     /locus_tag="IV142_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486195.1"
                     /translation="NQEINPTTHFMSNKLTIELDNSNNKNTVLTTPIYRYKGQVASIN
                     GKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYLSIVTLILFLLYRFTFSK
                     QKQPRREVQHSH"
     gene            complement(490..1230)
                     /gene="truA"
                     /locus_tag="IV142_002608"
     CDS             complement(490..1230)
                     /gene="truA"
                     /locus_tag="IV142_002608"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP8486196.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(1421..2056)
                     /locus_tag="IV142_002609"
     CDS             complement(1421..2056)
                     /locus_tag="IV142_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP8486197.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(2203..3444)
                     /gene="sstT"
                     /locus_tag="IV142_002610"
     CDS             complement(2203..3444)
                     /gene="sstT"
                     /locus_tag="IV142_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP8486198.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            3642..>4222
                     /locus_tag="IV142_002611"
     CDS             3642..>4222
                     /locus_tag="IV142_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486199.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSI"
BASE COUNT         1414 a          852 c          635 g         1321 t
ORIGIN      
        1 aaccaggaaa taaatccaac aactcatttt atgagtaaca aacttacaat tgaattagat
       61 aattcgaata ataaaaatac tgtcttgacg acacctattt atcgttataa agggcaagtg
      121 gcttctatta atggcaaact agtacaaaca aaattatcta agtttggcac aactgaatta
      181 actattcctc caggtataaa taaagtcgtt attacttatc agtacacaaa actagcaatt
      241 gcttcaagat atttatctat cgttactttg attttatttt tactttatcg atttactttt
      301 tctaaacaaa aacaaccgag aagagaagta caacattctc actagatttt tatttaagaa
      361 agacttttta gctgtacaca taaacaggct aaaaagtctt tttttatatt tctttaattt
      421 gcttcataca ttatttaaca tcgatattta tactgtgaaa catgcgtttc atccattatc
      481 aaagtatttt tatcgataat aaacttcatc caaaaacaga ccttgtgcag ggacggtttc
      541 tcctgcatgt tgccgcactt tgctttcaaa tatttcatcg attgtttcta tcggcatggc
      601 acctgtgccg atttctagta atgtccccat gataatccgc accattttat ataagaatcc
      661 gtctccgaca aaagtaaaat gaagcatttc tccctttcgt tcgatagata attcatcaat
      721 cgtacgcact gtggattttt ttgatttttt caatgcagaa aaaccaatga aatcatgtgt
      781 acccactaat ttttcgcaag ctgcattcag cttgtccata tccaactctt gagggaaata
      841 aaagctgtgg tttcgctcaa atgctgaagg aatcacgttg ttccaaacat aatagctgta
      901 ttttttgccc accgcattat atcttgaatg gaagcgctca ggcatttctt cgattctttt
      961 gatgacaata tcccgcggaa ggtaacgatt cataaagtcg atcatttctg ccagtccaat
     1021 ttctgtgttt gttttaaaat ttgctacttg tcctcgcgca tgggtgccag catctgttct
     1081 tcctgatcca ataatttcta tctcttcatt tgtcatctga tgcaatactt tttcgatttt
     1141 tccttgaatg gttttatcgt catctccaag tctttgccat cctgaatatc tttttccgtc
     1201 atattcgatt gtcaacttga tgtttctcat attttagtgg ctcctctgtc ttccatttgg
     1261 tcttctctct acctttttaa gattgtagca caaaatatag ctccttgctt ctctaagaca
     1321 gaagctatac aagaaaactg tggaaatata tatttcagaa aaacaaaaaa cagccaccgg
     1381 atgaagtatc cgctgactgt tcaatttgac ggctcaagtt ttactgtttg agatgcaaga
     1441 tactcaacca ggcaatgggc acataattaa agaaaaaact gattctttgg caaaaaccta
     1501 ttttattcag taatggccaa gaatttatag aaggaattct tgccagtcca taaatcgccg
     1561 caaaaagcaa actgatcaca gacagtccaa ggtataagtg gcttacgtcc atttgcccat
     1621 tttttcgata gatcaagaaa agaagaactg gaaacaataa aaaaccactg taacctaaac
     1681 ctgatccgat attatggacc catgtcgaga cattccacga agattcatgg gtattgatac
     1741 tgaacaaacc tgtaaagata caatcaccta taccatagaa cgcgatggct gctgtaacta
     1801 gggtagctaa cacacgagat gtactaacaa atgcagcata caatgcaggc aatgacagga
     1861 tgaaaaagat ccctgaaacc acagaccaaa taagaaacgc ttttcttaca ggactggaaa
     1921 cctctccaaa tacactgatc acgctcttca tttgatctaa ttcaggataa tacaatccta
     1981 atacgtacgg agtcaaaaaa tcactcagca cgcctaataa taaaaagaaa aaaccaaatt
     2041 tacgtaaaaa atccatgata cgcctccaat aacaattacc tatgctttta attgttatta
     2101 taaacgactt tatcttgatt taacagaaac ctctatttac acgaacatct atataactca
     2161 ttaaaaactc gaatcatttt aaaaatacta aatatctaac gttcaacgtt cacttaatcc
     2221 atctaaacaa tctttcaatt tgattttttc cccacttctt ttacgcgcgc ctagctctcc
     2281 tattgcagta aacaacagat cacttgacga atttaaggca gtttccattg aatcctggat
     2341 aacaccgata ataaatccaa taccgacgat ttgcatcgcg acatcattag agataccaaa
     2401 caaactacat gctaatggaa tcaataacag tgaaccgccc gcaacacctg aagctccaca
     2461 agcagctaaa gcagacaaaa tacataaaag gactttcaaa acaaaaggtg cttcaatccc
     2521 taaagaatgt acagcagtca acgtcatcaa ggaaatggta atcgcggcac cgcccatatt
     2581 gatcgtagca cctaacggga tcgatatgga ataagactct tttgtcaatt tcaatctttc
     2641 agccaacatc atgtttatcg ggatatttgc ggcagaactt cgggtaaaga aagcgggaag
     2701 tgcgctttct ttcaaacaaa agaaaatcaa tggataggga ttttctttaa taaacaaaaa
     2761 cgtgaggaat gggtacacaa ctaatgcgac aaacagcatc gtaccgatca aaacaagtaa
     2821 aagctgcaga tactctgcca atccagcgat tccagtcgta gcaattgatt gataaaccaa
     2881 tcctaaaatc cctatcggag caaatgcaat aatcacttgt acgacttgtg acaacgctat
     2941 agatatctga tccaccaact cttttgtcga ttctgaacgt gctcgaaaag ctagtcccaa
     3001 caatacagcc cagaataaga cgccaaggta attcgcatca ataagagatt gaacaggatt
     3061 ttgtgtgaca ttcgtcaaca tagtagataa aacactttcc aaactttgcg gtgctttttc
     3121 agatactgtt tcttgtaaag taataggaac agtaaaaatt gaacttacaa taactgctac
     3181 aagtgcagaa agcaatgttc ccactaaata taagatcaaa ataggcttca cgaatgtctt
     3241 attcccaatt ttatgcttgg caattgacgc catcgtcaag aaaaaaacca gaataggtgc
     3301 gattgccttc aagcaactga caaacaacgt tcccaatatt gaaataaatg tccatgtggg
     3361 gaaaaagata ccaaacaata ttccgactaa aataccaaca acaatacgtt gaactaacga
     3421 catatccaaa aacttctcta acaaatgacc accctttcac aattttaaca atcattgtat
     3481 cattttatgc aaagaaacta aaggtataat tttctaaaaa ttctaacaat acaaacaacc
     3541 atttcttgct aattttgtac tttttctgtc tacacgttgt tttttgagat attctaagtt
     3601 aggatgttat taatgggcaa cttatcagga aacgttttct tatgtttttg tccaattttt
     3661 ttgtgaggtg tttattcttg aaaaaaataa agaaggagag ttatggcttc ctgacaatct
     3721 ttgtcgcttg tataatgatg gttttacagc gtatattgat ttcagcagtc actattccta
     3781 catatgcaac aaaccctggt cgattctaca tctatacatt tttacaaact tccgttgtta
     3841 ttggtgcaaa tttgatccca ctttacatgg gttaccaata tcaaaaaatc gcccgtttaa
     3901 aagttttaca ttatttaagt cgttttgcct ttatattcct aatagccaca ctagtttgca
     3961 atatcttttt ttacgtacaa gctggcagct tgaatattcg tgattattgg cttatcctaa
     4021 cacctatcag tcagaattac tttacttatg ctgtttcttg tactttgctt ttttgtagta
     4081 ttccttatac agttggattt ttaaataaat tatcgaaaac aacaattaaa tatctactat
     4141 tatttttaac aattggatta gttggctgct ctactctatt taataaagac ccgtttgcat
     4201 tacagaatgg acagagtatt tt
//
LOCUS       DACDEG010000139         4174 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.144, whole genome shotgun sequence.
ACCESSION   DACDEG010000139 DACDEG010000000
VERSION     DACDEG010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.144"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            439..1407
                     /locus_tag="IV142_002612"
     CDS             439..1407
                     /locus_tag="IV142_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8486200.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADARNHDLDVRRYMGKVEY"
     gene            1418..2233
                     /locus_tag="IV142_002613"
     CDS             1418..2233
                     /locus_tag="IV142_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAP8486201.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKKIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2301..3011
                     /locus_tag="IV142_002614"
     CDS             2301..3011
                     /locus_tag="IV142_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8486202.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3047..>4174
                     /locus_tag="IV142_002615"
     CDS             3047..>4174
                     /locus_tag="IV142_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP8486203.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1397 a          629 c          886 g         1262 t
ORIGIN      
        1 attttaagac cataaattgc gtactaaaag aaattctgca attcaaaata aagcatttta
       61 agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt ttccactcca
      121 gtagtttcct aattcaactg tgtgcaaaac gatttcttct aaacaaatca agacaaactt
      181 ataaaccttc atcccagcac cataattatt taaatcatag gcatttttcg aaattagtat
      241 atgtactcaa gtttcactgc agattattta tattttttag agaaataaat ttttcctatt
      301 gacaaacaca aagaaagcgg atacaataca attatgtcac atgacgttat atgacgttat
      361 gtaatatctt tacatttaat cagttacttt tattttggat aaaattaaca tttttaaaat
      421 tttaaggagg aaatgttgat ggatgtagca aaaacggtta acgcaattgt agaaaaaaat
      481 gatggagaga tttctggaat ttatttggtg gcttgtggcg gttcgctggt agatatgtat
      541 gtctcagatt attttcttaa agccgaagca tcgaagtgta ttacaggctt atatacagcg
      601 aacgaattta accatgtaac cccaaagcaa ttagatgata aatctgttgt cattctatgt
      661 tctcatggag gaaatacagc agaaacggta aaagcaggag aagtcgctca agctagagga
      721 gctgtgactg ttggtctgac tcataataaa actgcggatt tattaaaggt ttctgatcat
      781 agtttccttt acgaatgggg agatgatgca aaaatagtca ataatcctat ggccatcatt
      841 cttgatttaa ctgtatcttt ggtcaatgtt accgaaacat ttgaacatta cggtaaattc
      901 agagaaggca tgaaggttat cgataccgta attgaaaatg gcattaagca ggtgcaggat
      961 cgtattaaag tgtttgctga taagtacaaa gatgaaacaa tgttttatat cttaggtagt
     1021 ggaccatcat atggacatgc ttatggtttt tcaatttgct ctttgatgga aatgcaatgg
     1081 cttgattcaa cttctgttca ttctggagaa ttcttccatg gaccttttga agtaacagac
     1141 aaagatacga attttattct cttgatgagc attggacgca ctcgtgcttt agatgaacgg
     1201 gcaaaacgat tccttgataa atatgcagaa agagtagaaa tcgttgatgc aaaggaacta
     1261 ggattggata tgatatcaga tgaagtttct gaattcttca atcccatttt attctacagt
     1321 attctttcag aatatcgttc agctttagca gatgcacgca atcatgattt agatgttcgt
     1381 cgctatatgg ggaaagtgga gtactaggag gattaaaatg aaggtattag gcttaggtga
     1441 taacgtcgtc gataaatatg aaaatctggg aattatgtat cctggaggaa attcattgaa
     1501 ttttgcagtt tttgcaaaaa aactgggaca tgaaagtagc tttatgggag tttttggctc
     1561 agatgaggag gcagctcatg ttttatcagc aataaaaaaa ataggactgg acaacagtca
     1621 ctctcgcttt gaaaaaggcg agaatggctg tgccagagtg aagattgatg agggcgaccg
     1681 gattttcctt ggcagcaatt cgggaggtgt tactcgtgaa tacccgattc agcttactga
     1741 agaagacaga gaatatttaa accagtttga attgattcat atgggattat atagccatgt
     1801 caatcatcta ttagaagagt tacaaaatgt ttctggaaag atttcttatg atttttctga
     1861 cgactttacg gaagaagagg ttcagcgggc aattgataaa gttgattttg gcttcttttc
     1921 tatagaagac ttttccgatg aagaagtaaa gcagtttttg atgaagcatt tttgtgagcg
     1981 aaatgatgta ttgattgcaa cccgaggaga aaaaacggct attggttatg atggagcaaa
     2041 gttttatgaa gtgaaccccg tgctagaaga accagtcgat acaatggcag cgggagattc
     2101 tttcttaaca gctttcctac tttattttct cgaaggggac gatatagtca ccgcgatgaa
     2161 aaaggggaat gaatttgctg ggaagtcttg tctggttgac ggttcgttcg gctttggtat
     2221 tcattacgat tgagcttgtt ttaaattcct gcatttgata acctaaagaa agaaatcccg
     2281 gtaaagtgga ggttaaaaaa atgctggata caaatgcaca attaccattg tacttacaat
     2341 taaaagaaat attaaaaaat aaaatcaata caggggagta tcccgagaat acgaaaattc
     2401 ccgctgaatc agagctgata gaaagttatg aggtcagccg aatcacagtg agaaaagcag
     2461 tagaagaact ggtagaagaa gggtacctac ttaaaaaaca agggaaagga acttttgtca
     2521 accggcataa agtttttcgt aaaattgaat atgttatggg tttcagtgag agttgtaaat
     2581 ctaatggttt tacaccgact agcattgttt tggaacgcaa gatcattcca gcaactgaag
     2641 ggatggcaaa aaaactcgat atcgctcaag gtgatgatgt tttatatatc cagagaaagc
     2701 gaatggcgga taacatcccc attatgttag aaaacaatta tttttcttat gaaagatata
     2761 gtttcttaat ggaggaagag ctgactgatt ctttgtatga agtacttaaa gtccaaggta
     2821 ccttagccat caatcctggg gaaactgcac ttgaaattgt cacagctgat gaccaaattg
     2881 ccaaaataat ggaagtagct gttgggacgc cctttttctt tatggatact ttaatcaacg
     2941 atcaaaacaa cataccaatt catgtaggta atcaatatta cctgggagaa gtatatcgtt
     3001 tttacttatg atttcctgtt ttgatttcaa ggagtagatt ttatttatga agcaaatatt
     3061 gcggtcaaaa atgatttttg atggagagca gccacctttc aaaggttata ttgtaatcaa
     3121 tgaagggatc attgaaaagg tggaaaacaa ctggaattac agtgatctat taaatgaaaa
     3181 cacggaatta attgcttatg atgatcaatt cgttatgcca gcaattcatg ataatcatgt
     3241 atttttcagt ggttatttgg ctatgaatgc tggcatagat ttaagcgata catcttcagc
     3301 taacgaggct gtagagcgta ttaaagctgt aatgaaccat aaaaaagcag atgaaccaat
     3361 ttatgctcat ggctgggatg ctgccctgtg gaaaactgac ccatcagaag agctattgaa
     3421 tgaaataaac cactttggtc caatcacagc gatcgacaaa aatcgcagcc attgttggat
     3481 gaatgctatt gctaagcaga cttatggttt tacagaaaaa gaaaccagtg ctgaaagtcg
     3541 agtattatta attaaggaaa tgctagcaaa caaaaagctt ttagcagagg tttacgctga
     3601 atttgaagca ttactgcttt caaaaggtgt tatatcaatg aaagaaatcg tttttgatga
     3661 cgcagaattt ctttctaata tacctcagag gaaaatactt agtaacctat atgtgcaggc
     3721 agtccaagaa cctctacagc atgaacttct atatgagtat aaggaaaaag ttttccctga
     3781 aaaggtccgc tttggtggag tgaagatcat ggtagatggt gttgttgcgg atagaagtgg
     3841 agatatttac ggtgtttatc agtcaggagt agcttcaccc gagattgact atgaggcaat
     3901 taaagcagaa gttgctgcat tcaatgaagt agagattcct tgctgtttga caacggaagg
     3961 agataaagcg ggttataaag ctgcgtcaat tcttgtcgaa tttggaaagc ggttacctga
     4021 aggcgttttt aattcgatta gtgatttgga aatggttact caaaagacag cagaaaaaat
     4081 gaacgaagga caagtagtag cagaggtata tccacaaatt ctcggcttga atcaaacgtt
     4141 agaagaggct tacatgtcaa ctgttataaa aaat
//
LOCUS       DACDEG010000140         4073 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.145, whole genome shotgun sequence.
ACCESSION   DACDEG010000140 DACDEG010000000
VERSION     DACDEG010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.145"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            84..581
                     /gene="dfrG"
                     /locus_tag="IV142_002616"
     CDS             84..581
                     /gene="dfrG"
                     /locus_tag="IV142_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP8486204.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(653..2605)
                     /locus_tag="IV142_002617"
     CDS             complement(653..2605)
                     /locus_tag="IV142_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP8486205.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2612..2908)
                     /locus_tag="IV142_002618"
     CDS             complement(2612..2908)
                     /locus_tag="IV142_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486206.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            3086..4073
                     /locus_tag="IV142_002619"
                     /pseudo
     CDS             3086..4073
                     /locus_tag="IV142_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1257 a          706 c          674 g         1436 t
ORIGIN      
        1 ctaacgggca ggataatgag aaaaggtttt ttgatagaaa taagaaatta gatggtaaat
       61 tagttaaaaa ggctgtgtta taaatgaaag tttctttgat tgctgcgatg gataagaata
      121 gagtgattgg caaagagaat gacattcctt ggaggattcc caaggactgg gaatatgtta
      181 aaaatactac aaagggacat ccgataatat taggtaggaa gaaccttgaa tcaatcggaa
      241 gagccttacc tgacagaaga aatattattc tgacgagaga taaggggttt acctttaatg
      301 gttgtgaaat tgttcattca atagaagatg tttttgagtt atgtaaaaac gaagaagaaa
      361 tttttatttt cggaggagaa cagatttata atttgttttt cccttatgtt gagaaaatgt
      421 acatcacaaa aatacatcat gaattcgaag gagatacttt ttttccagaa gtgaattatg
      481 aggaatggaa tgaggtattt gcccaaaaag ggataaagaa tgataaaaat ccgtataact
      541 actattttca tgtatatgaa agaaaaaact tattgagtta acgggtgcgt taattgaaga
      601 agaacagtaa agataaagca caagagcttg ggattactcc aagctctttt tgtcatttga
      661 aataaattat atctttccaa tcaaattgtt tatctgtaat ccctaatctt tgagcaggtg
      721 tcaaaacctt tttatccgct gatttaaaag ggcgacaaaa gttgtagtaa gttcttagaa
      781 ttgtaatcgc aaattgagca tacttcggat tgaagttagc gtaaatatag ctcttcttat
      841 caccacgagc tgttgttaat ggtctttcaa gagatgatat ataacgtctt atctgttgaa
      901 taaagctatt ggtcgaatgg tcattaacat ttaatagcat ctttgcgatt tcgtttggtt
      961 ccagagcaga taaatccgtt ctacaatcaa ctgaataaaa tcctttatcc ttagtggcta
     1021 atggatgttt gatagggtta tcggcatatt tgcgatatct ttctccatct ttactaatag
     1081 cctcttcgtg gaattgatga cttgttagta attcagttag ataatgagat gccaaagtcc
     1141 gtagaaactt tgttttgaat cctctgatat ctccccaatc gagcagttct gcttttgctt
     1201 gtccgaattc ttttaagcat tgttttctgc ttttcgtctt gttaacttgg caaataaaat
     1261 gatgtgcatc gctcaacctt aattctttcg tgaatactct ataaaaggcg tttctaatag
     1321 agaagtcatc atcactaatc attcgccatt cgtcggcatt aaccatttgt ttaattaacc
     1381 aatagtgagc agttgtagta taaggagcat caacatttaa accatcgaca aatctactcc
     1441 tattagtaat tttccctaat tcgtgaagat aagcattcct attttcgcta tcgttagcgg
     1501 atggttcttg tggataataa gaccaatcta acctatcatt tttacgacta aaagtattga
     1561 gatggtcttc tttgaatttt cttgtatctt catttaattc atccattgaa atattccaat
     1621 cataggcaac atcagaacgg aacacatatc ttgataagac ctcagcagat acaactatat
     1681 acgtttgcat attaaggtct tcaaaccccg tatatttctt tgaaccttgt cctttcttac
     1741 ggacgttatt taggtagtag tgcatcttgt ccgtattaag ccacatctcg ttaaatttct
     1801 ttgtttgaag gggttgtgtc tcatatcgtt ctaaaaactc caaacaacgc ctatacagcc
     1861 actccagctt atgatagtag gttccaactc caattcctaa aatatcacaa gtacgactga
     1921 ctgaaaccct tccaacaacc atcttagcga acattggaag aatggtgttc ttttgttggt
     1981 gataagtagt ggtttcttct ctttttggta gaacattagt gaacttctta cacgccttac
     2041 attgatagcg ttgagatttg cccctacttt tccccctttt gtaaaattgc ttcggctcat
     2101 tgaatggggt tgattcttct tccgagcagc cttctttatg aaattgataa tctggctcaa
     2161 catcttggat gctattgatt tctataaggc gtttaatttc ttcaacaaca gaccaattag
     2221 ataaaggggt tactgtacag ttttgcgata cacctctgcc aataggattg tcattgcagt
     2281 acataccttt atgaccttta tctttggaac taccagtcat tttatatcga cttggtttcc
     2341 cttttacatc tttgaatttg tgctgttctt tcccaaaatt acaacaaaac ggattgatgc
     2401 agtaattata ttgaatggga tattcagaac cgttccattt aaaagacgtt ggcaagaaca
     2461 acattttgtt ataccttgtt gcaaatcgtt ttggagttaa taaatcatat tgtttagccc
     2521 tatcttttaa ttcttcgttt gaaatcggaa ttttaacatc tacaatttca tcttccttcg
     2581 tggctaatct tttaagcttg gtcattgtct atcaacccac tctatctttg atacccattt
     2641 tttcaacaat ccgcttatta cttcgcttgt tggcaatcca ttcaataaac ttcttatcgt
     2701 ctaaaatgtt caataagaat tcatccactg cttcgctttc agtacgctct gcatattcag
     2761 cgtattgctt aactatcacc cttacttgct ctgatatttc ccaatccaca gacttagcgt
     2821 tcttattttt tggctcaaga aattgcatag gaaaatgcac ccctttttgc ttagtaaatt
     2881 gtaaccattt taacacacca atttgcattc ctacaataat tatttcacgt tcgcattttt
     2941 atatacattt taacttacat tttgtggtgt aaattaacaa caaacgttga tttaacaata
     3001 aaaaacgagt atcaaattca gttgcaattt gatactcatt taggtttaca ttttcacttt
     3061 ttgtactcca aaactgaact acttattttt atttagttgt ctctatattt tctctgctta
     3121 tcggacagta gacatctact atcgttccgc aattgccgaa gctatcgcaa taacacttgt
     3181 tatccctgta ttattttatg cttatcaaat catctctgga aaagaagaaa agtatccgtc
     3241 tgtaaagcta gcattgagta tgagtttgct agtgtacagc catgtactat caacattaat
     3301 gagtacagca ttaattatca tttttatttt tattcgtttg gtttctaaag gtttcaagaa
     3361 tgcagatttt attgctatat ttaaaaaatt atttagtgcg gcaggaatga cattggttct
     3421 gacgagtgct ttttggtatc cgatgtttga acaaatgctc taccaaaaaa tcaacaaacc
     3481 tagcgtgaca aatttatatg ctcatgcatc taatgtgttc gatagtctga ctgaagctat
     3541 gaacaacgac ttaactactt actcaatggg actagttggt ctgttatctt tatgtattcc
     3601 cctgatttta tttaaaaaat taacaaacat agaaaaaaag atttattatg gtacgtgttt
     3661 gacttggcta gctacaacta gtttggtgcc atggtatctt ctgcaaaata cgccagccaa
     3721 attgttacaa tttccttggc ggatcttaag cttgcaaata atattctctt ctctgatatt
     3781 aacgatgata tttttcaaaa atagaagata caataagaca agagaactat tttatttatt
     3841 tatgactgtt ttgttattaa tcactttaac tgtttctagt aaaaaaaatt tcaatcaaaa
     3901 aataatttct cagccgggac atattgttgt aaacaaagaa acagtagagg cgtataccac
     3961 taaaggaatg gattattttt tatatgatta ttctcctatc gcttcattgg agaataagga
     4021 gcatataata aaacacgaag caaagattgg cagtcaatgg gtaaaccttc ctt
//
LOCUS       DACDEG010000141         4003 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.146, whole genome shotgun sequence.
ACCESSION   DACDEG010000141 DACDEG010000000
VERSION     DACDEG010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.146"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            537..2030
                     /locus_tag="IV142_002620"
     CDS             537..2030
                     /locus_tag="IV142_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP8486207.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            2165..2461
                     /locus_tag="IV142_002621"
     CDS             2165..2461
                     /locus_tag="IV142_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP8486208.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            2562..3413
                     /locus_tag="IV142_002622"
     CDS             2562..3413
                     /locus_tag="IV142_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486209.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            3434..3646
                     /locus_tag="IV142_002623"
     CDS             3434..3646
                     /locus_tag="IV142_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486210.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         1423 a          580 c          807 g         1193 t
ORIGIN      
        1 ctccaaattg ataagaattc agactttata aggaatatag tggatatact aaatatattt
       61 gctatattca gtatatcaga tatattcaaa atgtcaaaat atatttgtgc gttttgcgtt
      121 gacaatttag agtgaaaaga ggtaaactta attctataga aaccacagat aattcactat
      181 ttacaaagta gcaaaagcag tggtactctt taaacagaaa aattacataa aaaaaagaga
      241 cggaaataat tgattttagc agaatcaatt atttctgaat cacatggtta ggacatgtgt
      301 tcacgctcgt ctcaagaata tgtaagtagc taccaactac ttatattaaa agttgatcgg
      361 ataccttttc cgattgcctt tattctacca aaaaaaggcg agcaactcaa cagaagaaag
      421 tttatttagt gtacttaaat taaatattct taaacactga agagagaaca catactatgg
      481 ctgagattaa agctaaactg tgttctcttt ttgtttgaga aaggacgtta caatcgatgt
      541 tgagcgaaat ttattcagct attcttaaaa acggcttacg tacatacaaa tatcgaaaca
      601 gcaccatgaa acctgcaggt catattgatt ccagtaggag tggtgctatc tttgcgtacc
      661 gtagcaagca attaatgaat gttgggcgtg gtatggtcat tacttcggaa gaagctattt
      721 tagagaacga aaagaaacta acgcattgga cacctaatac ctatcgcttt ggtacttatg
      781 cggatgatta tcgaacagtg gttaaaggtc attctgagaa aaacttaagt caaatcaaca
      841 catttgtagt tgatatcgat tcaaaagaga accatcaagg cgaaattatt ttagcctgtt
      901 tagatcaagt gggctacatg ccaacgctaa ttttagaatc agatcatggt tatcaagttt
      961 attttgtact aaagacacca ggctatgtca caaagaaaag caatttcaag gtgatcgatg
     1021 tagcaaaaaa aatttcaaaa acaattcgtc tacagttggc tgaaaaattg tccggagtgg
     1081 atctaggatg taaccacttc ggaattgctc gattcccaca agaaaataat attgttttta
     1141 aagaaatgaa ctatcagtat tcttttgctg actggattca atggtcgatg aagcttgaat
     1201 cagatcaaaa aacaaaagaa gatcgagaac gaaaactaat tgtctttcct gagaaaaaag
     1261 aaattcgtca agtagatgaa ccgtggttcg atatgttact tcataaagca aacatcgtcg
     1321 gcggagaagg acgtcttggt cgaaataatg tgattttcac gctttcttta gcttattact
     1381 cttcaggtta tgcattggat acttgcgaat acaatatgtt cacattcaac gaacggttac
     1441 aggaaccgct atctgaagca gaattagtaa agattgtacg tagtgcttat tctggtaatt
     1501 atcaagcagc aagcagagaa ttcattattg aattgtgcca gcaatgggtt tcaccaacct
     1561 taaaaaataa tgatctgttc attcaacgac gtggctggtg gaaatttaaa aagccacgag
     1621 atcaaagaaa atatagtcat aaaaatgaat ggaaagaaga tctattggct tatctaagcg
     1681 aaaaaagtct tgctagtgag ccttatttga acctgtcaaa aaaagaattg gcttcacaac
     1741 taacgatgcc caagcgaaca cttgataaga tcttaaatga gttacgagag gagaataaag
     1801 tcttctacaa ggtaacacga ggaagaaatg gcggaatgat cattgcttca gtcaaaacgt
     1861 tgttcgcgag ggttattcaa ctaagtaaag aaaagagact agattattca acaaggatca
     1921 cagaggtatt tggactcaac gagagcttag tagaagagac atttagacaa ttatcaaccc
     1981 ccttgaaccc tcttaaagaa gtgaatcttt ttgaagtaga tacaggttga aaaataaaag
     2041 gtgcaaattg ccctatacaa tatatctatg atacttctgt aggtgttgct ccctttgcct
     2101 agctacgtgt aggtggtata cttataactg cgtatgcagt cttatgtatc atggaggaag
     2161 ttgtatgtcg ttaaaaaatt atgtctattc acatcctgtt aatgtgttta tcattggtcg
     2221 tttgggatta tctgtggaag tgttctgtga gctttatggt tttaagcagg ggactttgtc
     2281 ttcttgggtt accagggaaa aaacagttgc ttctttgccg attgagtttc ttcatgggtt
     2341 gtccttagct tctggggaga ctatggatca ggtatatgat tgtttgtgtg tgttagagca
     2401 ggagtatatt gagtataaaa tagctaacga gttaagaaaa aggaagaagt atatccagta
     2461 gtagttttta tttacttaaa aaattggttc attgagcaac ctatcgctgc taaagtagca
     2521 aaggaaaacg ttaaaggaaa gaatttaggg aagaaatcga aatgaaatcg gacgactgga
     2581 ttgaacacac ctggcttcct ctgaaaagaa atagcaaacg aaagaaattt tgggtattgc
     2641 aattatggcc attaggaaaa ctttgtagtt acggggcacg acttattctt gatttaaccg
     2701 tttactcgat tggagtattt ggaacggctt attacttgat aaatgatccc aataggattg
     2761 cagcatcaga catttttaca gtgctaatga cgttgatttt tgtcatgcta ttgtctattt
     2821 ttactcaatt agtcatgcaa tttaccaggc aaaaattaac agcaggaaaa cttttcaaaa
     2881 gcctattcga tttaattgtt gtaaatgcaa ttctagcgac tttaataaaa caaattgaac
     2941 aatctggaag aacaacaaaa ggagtgaagg attttttatt gacgtttatt cactttgtaa
     3001 atggacacat gtatttgttt gaagtgggta ttattctctt ttttgtcggg tatgtatcca
     3061 cgaaacttat taaacgttca attgaaaaag aaattgaaat tgacctgatt ccgacacttc
     3121 ttggttatga gtacaatgtt gattttggaa gatatgacga attgatcaat acggatatcg
     3181 ttcacacgag tatccatgaa tctactgtta agggcatcag atctatttta aaaaatggcc
     3241 aggaaatgaa agcaatagat cgaagaaaag agatctacaa tttttatgaa gtcgaagcaa
     3301 gcctacgata tggcatcgga aaattgtggt tagatggaag taatattcgt caaaacaaac
     3361 catacatttt ttggaacagt agtgatttag aacttccaat gaaagataaa taattatgga
     3421 gaatcgtttg aaaatgattc ctctatacag gattgtgctt atagttggca tggagttaaa
     3481 aaccttaaag aaggagcagt tattcaaagt gaaagaacgt cataatcagt tactatgtag
     3541 taaaacgggt acgatgaaca ttgaataccg aacagaatat ttagaattaa cgaatcaaac
     3601 tgattttttg ttgattcgaa agcaacaaga gcaagaaaaa aattaatggc tacttatagc
     3661 caattgattg atgaatggga acaattcaag gaatctaaaa ttccagaacg tctctaaaag
     3721 cctttatttg taaagagcat aaggaaacgt gggaacactt tttaaaaaaa gtgttccaat
     3781 aggctgtgcc tattacgttt ctcaaaaata aatgtgggaa caaaatattt aaaaatagga
     3841 tagtgaaagg atagtgaagg atagtaaaag gagggtagta ggatagcaaa aaggataggc
     3901 tccttttcgc tatccttttt aaattggagg aattaatatg gaagtaagaa taagaaatgt
     3961 ggatgagaaa atcgtaagag aaattgatcg aagatgtgac gaa
//
LOCUS       DACDEG010000142         3939 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.147, whole genome shotgun sequence.
ACCESSION   DACDEG010000142 DACDEG010000000
VERSION     DACDEG010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.147"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1372
                     /locus_tag="IV142_002624"
     CDS             <1..1372
                     /locus_tag="IV142_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP8486211.1"
                     /translation="SAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNLETFQHSA
                     IIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRWFAFPRLV
                     ILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSKKNNKHKV
                     EAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNTTFENLTL
                     INPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFTAILVKVT
                     SPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVGKFIRAVR
                     FDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGYAQVYGKY
                     VSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVISLAEIAFP
                     NEKIFR"
     gene            1388..2428
                     /locus_tag="IV142_002625"
     CDS             1388..2428
                     /locus_tag="IV142_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8486212.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            2447..3532
                     /locus_tag="IV142_002626"
     CDS             2447..3532
                     /locus_tag="IV142_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP8486213.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            3565..>3939
                     /locus_tag="IV142_002627"
     CDS             3565..>3939
                     /locus_tag="IV142_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAP8486214.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLD"
BASE COUNT         1415 a          552 c          728 g         1244 t
ORIGIN      
        1 gagtgctgca cgacggatat tcattatatt aatggatgtt attgtataca atgcagcaat
       61 atatagtagt ttcttactga agtttcgtgg agagatccca ttacgaaatt tagaaacttt
      121 ccaacactca gcaattatta tttcagtgat ttttattgcg ttgaacattt tgttgggtgc
      181 atatgttttt tataatcgaa tgatcagtga catcgtgttt gtcacagtca tcggtcaggt
      241 attgatgaca ctagggatca tggtagtgac ttttgcgggc cgctggtttg cttttccacg
      301 tcttgttatc ttactttcat ttgttctcgg tacgcttttc ttaagtatct atcgtattct
      361 cgtatataaa ctttatttac gcgtaagtcg agataaaaag gtcgtcatta ttgggttaga
      421 aaaggatgtc gcaccagcaa tccaaaattt tggttcaaag aaaaacaaca aacataaagt
      481 cgaagcagtc attcttgata actattatgc aaacatcaaa aatatgatta aagacatcga
      541 tattgtctat cttgcttctc atatcgaaga aactgaaaaa atcaaaatct atcaattagt
      601 tactaaaaat gaaaaaaaat tgtttttgaa tacaacattt gaaaacttga cattgatcaa
      661 tccaaatatt atgaatttcg aagatgaaag tattattgaa gcatctggat ttagaatcca
      721 acctgaatat gaactattca aacgtttgtt tgatatcatc gtttcacttg ttttattagt
      781 tattgcttca ccatttatgt tattcacagc aattttagtc aaagtgacat caccgggacc
      841 aatcatctat aaacaagtgc ggatcacaaa aaatcaaaag gaattttcaa tctataaatt
      901 ccgctcgatg accgccacag ccgaagcaaa atctggacca gttttagcta agagcaatga
      961 tgcacgagta acgcctgtag gtaaatttat tcgagctgtc cgctttgatg aattaccaca
     1021 aatcttcaat gtgctgaaag gcgatatgtc gatcgtagga ccacgtcctg aacgtccttt
     1081 cttcgtcgat cagttcaatg cagaaaaccc ttattactac ttacggcata atgttcgtgc
     1141 tggtatcact gggtatgcac aagtctatgg aaaatatgtc tctgattaca acagtaaatt
     1201 acgttttgat ttactttata tcaaaaaata ttcattgatg cttgatttaa agatcttgct
     1261 gcaaaccatc aagatcctat ttgataaaat gtcctctcaa ggacttgaag aagacgaaaa
     1321 tgtgatctca ttggcagaaa ttgcttttcc gaatgaaaaa atctttcgct agttgggagg
     1381 cgtaaaaatg aacaatccga aattcagcat aatcgtacca gtatacaatg cagaagcaac
     1441 tattggtcga acgattgata ttctaaaaga attggattat gaagagtacg aagtcgtttt
     1501 agtaaatgat ggatcaacag atcaaacaca ggaaatcatt caagaactga ttgaaaatga
     1561 tgaacgtttt gaattgatta ctacgaccaa tcaagggccc ggattagcaa gaaacgaagg
     1621 aataagacag tcaaaaggag aatatctctt attcttcgat gcagatgaca cgccagtaaa
     1681 aacgatcttg tcagattatg atcatttgct agaaaagagt ccaaaagcgg atcttgttgt
     1741 tagttcgttt gtttttcgta caatggacga gggaagaatc gtttccgaaa aagaaaactt
     1801 agttacagaa tgtcactatc taactcacga agagtttata aaagacatgt atcagttaat
     1861 gaatgaacag ttgatgtatg ttgtatggaa caaatgctat cgtagcgata ttctgaaaaa
     1921 taaccaaatt ctatttaaaa cttataatag ttgtgaggat cgaatattta acttacatta
     1981 ttatgaatac tgtcatcaag tgatcatgaa cccaaaagta gaatatattt atgaatttga
     2041 aggtgggaag gggatcacta atcaataccg acctaacaag ttcgaaacat ttaaagaatt
     2101 ctattcatta gcaaatactg taacagacga tattaataaa gacggtatgg cggcgcttct
     2161 tttaaaaggg acaacttcag ttattttttc tatttatagt acctctgcaa gaacttcaaa
     2221 agagaagaaa gacgaagcaa agcagatatt gagtgatcca gtgatccgag aagccaagca
     2281 aattgctaag acggattctt cagcaaaaaa aataacaaag ttgctttaca atatgccagc
     2341 tccactattt ttatctgctg taaaagttgg aagcttcgtt gaagtgaaaa tgccagggtt
     2401 aatggctgct ttgaagcgag tatattagtt aatggaggaa aatataatga aaagaatttt
     2461 gcattttcag ggacgaatgg gtttaggtgg agcagagtct ttcatgatga atttatatcg
     2521 taaaattgat cgtacaaact atcaatttga ttttttgatt tatgaagatt atgcagatgt
     2581 tcaagactat cattcagaga ttgaaagatt aggaggccgt atttttgttg tacctaatcc
     2641 taagaaaaat atttttaaat atttaataga agtgaataag ttactaaaaa aagaatcttt
     2701 ttctatagtt cacaatcagg tttattttgg cgggggaatt aatttatggt tagccaaaaa
     2761 gaatggaatc agacagagaa ttgctcatag ccatgcaact gaagatggaa aatctcaaaa
     2821 tattgtaatg aatgtattgc gtaaattttt aaccaaattg ttgttacaaa atgcaacaga
     2881 ctaccttgcc gttagtcaag aagctggtga aagtctgttt caaaaccatc cctttgagat
     2941 agttcataat ggtattgatt tagagcttta tagtaaaaat tcagaagcta aggttaataa
     3001 gagaaaagaa ttggatatct caatgagtac ttttgttgtg ggaaacattg gacgattaga
     3061 gaaacaaaaa aatcaatctt atttgttaga gattttttta gaaatactaa aaatgaattc
     3121 tgattcttac ttgattttag taggtgaagg gtcacttaga aataaactga aagccaaagc
     3181 agtagaattg gatatagaga aaagagtaaa atttttagga gaaagaaacg atattcccga
     3241 gttattatct gtgatggatg tttttgtaat gccttctctt tatgaaggtc tgccaatggt
     3301 tggattagaa gcacaagcag caaaattaaa attagtctta gcagatacta tttccaaaga
     3361 tacaaagtta acaaataatg tggattttgt atcattagat gaaacaccac aagaatgggc
     3421 aaaaaaagta ataaaagagc cgtatgctaa taaaataaca caagaactaa agagttatga
     3481 tgtttcgtac actactgaac aaatggagag tatatatagt agagatcatt aaaaatgttc
     3541 aagatatttt aggagtgatt atttttgaaa atatgttatt tgatattggc atacaatgat
     3601 attggcaact taaagagatt ggtcaatcga ttgaacgaaa atgcagtttt tattattcat
     3661 atagataaaa aaactgatat agaaccattt attgaagcat ttaaatcata tgataatgtt
     3721 gaatttttat gtaataaaaa ccgtgttaaa atttattggg gtggtttttc cattgtacaa
     3781 gcagaaatca atttagtaaa gagagcatta caaaatgaga aatatttaaa atatgtatta
     3841 ttatctggtg ctgattatcc aatcaaagat aatgagtata tttataatta ttttaaaaag
     3901 aatagttcag tggagtttat tcgtggaata gatctagat
//
LOCUS       DACDEG010000143         3916 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.148, whole genome shotgun sequence.
ACCESSION   DACDEG010000143 DACDEG010000000
VERSION     DACDEG010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.148"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(25..1530)
                     /locus_tag="IV142_002628"
     CDS             complement(25..1530)
                     /locus_tag="IV142_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8486215.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1527..2213)
                     /locus_tag="IV142_002629"
     CDS             complement(1527..2213)
                     /locus_tag="IV142_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8486216.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2406..3827)
                     /gene="gndA"
                     /locus_tag="IV142_002630"
     CDS             complement(2406..3827)
                     /gene="gndA"
                     /locus_tag="IV142_002630"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP8486217.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1082 a          837 c          676 g         1321 t
ORIGIN      
        1 aaaaaaacaa gtaaagcaca tgctttactt gtcttctgcc tttgcgatag gaataaagat
       61 tctaaaaatt gtcccttgcc ctactacact ttctgctaag atacgtcctt tatagttttc
      121 aaccagttgt ttagcgatag acaaccccag accatttcca cccttcgttc tagctcttgc
      181 tttatccact cgataaaaac gattgaagat cttattaagg tcttctttgg gtattccttc
      241 gccaaagtcc tgaatcgcga tctcaaattc attcagagtg gaagaaatag agatatggac
      301 ttcttttcgt gtagttgaat actttactgc gttatccaat atgataatga ttaattgttc
      361 aaagtgatta cgataaattt gcagggtcac ttcttttgtc aaatcatcat ctaaagtgat
      421 cacatagtct ggatataata tcttgaaatt attataaact tgataggtga cttcttttcc
      481 tttagttgtt tcattcgcat aatgaacatc tacttgttct gctcttgata agtctagcat
      541 ctcttgaact aagcttttca tacggctgat ttcttgcatg ctggcgctta atgattcttc
      601 taatatctca ggatcatctt tcccccaacg atttagcaga ctaagatgcc cttcaataat
      661 tgcaacaggt gtccttaact catgagatac gtcttccacg aattgctctt gttgttcaat
      721 atacaaacgc atacggtcaa gcatctcatt gaatatttca gctaaatctg ccaattcatc
      781 atttgtgtcg atttcaggca tgtggatatc tgattgcgga tctttacgta tagtatccat
      841 tgtatctctg agtaccttga gtggttttaa gaaataagaa gataaaaaga aacctaaaat
      901 actacttaag atcaaagaaa caacttctaa aacaataaga gtcaataata gcttatttcg
      961 aatatcatag aaagaagata attcatagaa gctttgcgca tagccgatct tttctcttgt
     1021 acctttagaa tatataggct ggatagataa aaagccagtt tttccttcta gtgtaacgat
     1081 cgttggaact ttacgtgtag tctgaatcag gtttcgactg ttttcatggg ttttaaaaat
     1141 caagttctcg tccaaatcat aaacataaag atccattgat ggctgcccta gctcagctat
     1201 aaaggaatcg atcttcatta agtttccttc gaccagtgaa gtatgatcta cgacctcatc
     1261 tccgtcattt gtcgctttcg tcagattacg atacgtattt actaatgtca gttcttcatc
     1321 cgagttagcc aaacgagaag ctacttctga aatcgtacgc tctacattgt ttctttcttt
     1381 tgccacaatc agattgatag atgatttata ggtaatgaca gcgaaaattg taaacacaac
     1441 gaatatgaag aaagaactcg caaaggccca cttgattgtc aaggagggcc ctttcagttc
     1501 tcttttttcc gattttttcg gctttatcac gagcgcatca cataacctgt tccgcgtaca
     1561 gtttggatat agctttcctc cccgggaacg tcaattttgt tgcgaagata acgaatatat
     1621 acgtccacta cgtttgtttc aacttctgtt tcgtatcccc acactttatt tagtaaaacg
     1681 tcacgtgcta ataccacgtt cacgttttcc attaaagtaa gcagcaactc gtattctctt
     1741 tttgttagtt caattacttc atttccgcga cgtacgacac ggttttcttt ttcgatagtc
     1801 aaatcgcgat aagtaatagt agtttgttta gccacatttt tatcgccttc aatatcgatt
     1861 cgacgcaaca atgcacgaag acgtgctaat aattcttcga tagcaaatgg tttgacgatg
     1921 tagtcatctg ctccgtggtc aagtccagat acacgatcga tcacagaatc tcttgcagtc
     1981 atcataatga ttggtgtatt tttgacttgg cgtacacgtc tgcagacttc taaaccgttc
     2041 aattctggga gcatcagatc caacaatatg gcatcccact cattatttaa tgccgcatct
     2101 aaaccagttc ggccgttgta gtggacttct gcgttatacc cttcatgctt caactctagc
     2161 tcgacaaatc gtgccaagtt cttttcatct tcaatgatta gaatattgct catttattat
     2221 tttatccttt ctattctact ggctttttaa aaactctatc attataccga taatatgctt
     2281 aaaaatctat agtcatttta tcatggatag caattttaaa aaagagctga agtctactca
     2341 taacaatcga cttcagctct ttcgtcctct ttttattttc tcatttccta acagccgaaa
     2401 gactattatt cttcatggta ccatgagtaa tggaagattc cttctttgtc gacacgctca
     2461 taggtatgtg cgccaaagta atcgcgttgg gcttggatta tatttgcagg cagacgttct
     2521 gctctgtatg aatcgaagta agcgatggct gatgagaaag tcggtacagg tacgccagct
     2581 tggacagcca aggcaacgac gtctctgact gattgctgat attttttcgt gatttcaacg
     2641 aagtaatcat ctagcaataa attttcgatc tcaggatttt tgtcataggc atccgtaatt
     2701 ttttgcaaga atcgagctcg gatgatacat ccagcgcgcc agatttttgc gatttcacca
     2761 aatggcaagt cccaatcaaa ttcttttgaa gcgacacgta attgagcaaa tccttgggca
     2821 taactcatga ttttactgaa atacaacgct tctcggattt tttcgaccaa ttcttttttg
     2881 tctcccgtga actcaaaatc gttcgtacga gacaaaatct tgcttgcttg tacgcgttct
     2941 tctttataag cagaaatata gcgggcaaat acagattcag tgatcaatgg aagtggcaca
     3001 cctaagtcaa gcgcgctttg gcttgtccat ttacctgttc ctttattccc agctgcatca
     3061 aggatcacat ctactactgg tttacctgtt ccttcatcgt ctttacgtgt cagaatgtca
     3121 gcagtgattt ctatcaaata gctgtctaat tcgccttgat tccattcttt aaagatatca
     3181 gccatttctt ctactgaaag gtttaaaata ttcttcatta agtcataaga ttcagcaatc
     3241 agttgcatat ctccatactc aataccgttg tgtaccattt tcacataatg tccggctcca
     3301 tttgggccaa tatatgtgac acatggtgct ccgtcttctg cttttgcaga aatttgctct
     3361 aagattggtg caactagcgc ataggcttct ttttgtccac caggcatgat tgaaggtcct
     3421 tttaaagctc cttcttcacc accggaaacg cctgtaccga taaagttgat gccagagttg
     3481 gctaactctt cattacgacg catcgtatct ttaaagaatg tatttcctcc gtcaatcaaa
     3541 atatcgcctt tatcaaggtg aggaagtaac tcttggattg ttgcatctgt tgccggtcct
     3601 gctttgacca ttagcaagat ccgacgtggt ttctcgatag attctacaaa ttcttcgatt
     3661 gtatatgttg ctttgaaatt tttatctggg tgttcttcta ctacagcggt tgttttagcc
     3721 cctgtacgat tgaataaagc cactgaatag ccgcggcttt ctatgttcaa ggctagattt
     3781 ttacccataa cggccatacc gacaacgccg atttgttgtt ttgacatatg aactcctcct
     3841 acatacggga tttctcccta atctcttaca ttatagctta acacggctac attgcaaact
     3901 aaagaacaac atattt
//
LOCUS       DACDEG010000144         3858 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.149, whole genome shotgun sequence.
ACCESSION   DACDEG010000144 DACDEG010000000
VERSION     DACDEG010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.149"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            852..1064
                     /locus_tag="IV142_002631"
     CDS             852..1064
                     /locus_tag="IV142_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486218.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1342..3141)
                     /locus_tag="IV142_002632"
     CDS             complement(1342..3141)
                     /locus_tag="IV142_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP8486219.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3265..3855)
                     /locus_tag="IV142_002633"
     CDS             complement(3265..3855)
                     /locus_tag="IV142_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8486220.1"
                     /translation="MAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLVGD
                     SIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIFLL
                     GRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLTNM
                     SVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1382 a          683 c          670 g         1123 t
ORIGIN      
        1 tattaaatat tatacagata ataacggaat attatcatcg aaacagctaa caaaattgtc
       61 ataaaaaacg agatggttga tattagaact tgtgtttttg tttaggtcta gagataaaga
      121 gagacaattt ccaaatatat gtaatgaata caaaaagacg tttgtcgcgg attaccgtta
      181 caaacgtctt ttattgattt tccgagatct catcagtcgc catttggaat ggttcactcc
      241 ttatctagat tcgtttaatg ctcggactgc ttgttcgatt gtaataccaa acgcaacgtt
      301 attttcgttt tgtgcatcaa tcgccataaa acggttagaa gttgtatcaa aagctacgcg
      361 gtattgcata tttgtttttg taaacgtcat aaaattcatc tcctttgtaa tgtaatttag
      421 ttcttgaaag tttcctttaa actttcgaac ttataccaag tttagcatcg tacgtttttt
      481 gatgcaatta aaaagcattt atcattttcc ttatttattt tttcttaaat ggtttagcta
      541 ttttctttta atccgataag tagcccaatc atcaaggtta caaatactat tgcttgaata
      601 taaatcatat gctcacctct ttataaataa cttagcaata taaagaaaaa aacgcacgaa
      661 caacatagga atataaacaa acgtttttgt cacaacgtct aagatcgtgt taaaaaatag
      721 ttatctaaaa agcagaaaga tactcaaaat aaaaaataaa aaaatggata aaaacgtttt
      781 caaatacaag gtaatttgtt attataaaat aaagaaaaaa caaaataaaa ataaagaaaa
      841 gagggaatga aatggtaaaa gcagaagtag gaagatctta tatatgcaaa ccgatcggag
      901 tatctcatga actaatcggc tgtattgaac gtacatatat caacacggct gtcgtgtatg
      961 ttgaaagtta tgaccaacga gactatcaac ttatcaatga aagcaagtat cgcatgctcg
     1021 taaaattctg tgatattagc gcacctgctg aattggtttc ttaaacagta tttttgagaa
     1081 ttcgcaagaa ctcccttata atataaataa agacagaagg gagcgagaag atatgccaaa
     1141 aagaaaaagt tcagggaaaa tgggaattat tcttgcagtg atctctgcaa ttcctgctat
     1201 tttagctatt gttcgtgcga tgaaggaatc aagagaaagt taagcacaca ttatgagcgt
     1261 caaacaaaaa acgcatatac ctgcggtggc aggttatgcg ttttttgttg tataaagact
     1321 tttttcatta acgatttttg attagccgac tcccaaaaca aaatttagca gtttatctga
     1381 ataagtgatt tcttcatctg tttgtttcac actttcattt agtagtccca tctcatcggt
     1441 tatgatacca tccacaccat aaaacatcat gcgactcata gcatctgaat cgttgattgt
     1501 ccatacatat acttcttttc cgtcttggtg ggcagaatta atgaagttac ggttgatcgt
     1561 actatattcc attgtcagaa aatcagcagg cgtcacaggg ggcccaacaa tattgaaagg
     1621 taagatgtag cccacataaa agtttggttc tgcttctttt aaatctgaaa cggtttggta
     1681 cgtcaaactt tgcaaaatat gatggttctc taagataact ttgcggtatt tatggacaaa
     1741 tcgttcgaca aggtcatccg aatctttttt agaggtcttg atctcaataa gtaatttttg
     1801 gtttagctta tgtgcttttt ccaaataaga atcaaaagaa caaacagggg cttgcactcc
     1861 gttttcagta actgtaagat ttgtcaattc tttcaatgta agatcctgtg gtgttccatt
     1921 tacccctgtt aagtgacgaa gatttgcatc atgcatcact acgaaatgac catcttttgt
     1981 ttcctgaaca tccatctcta cataatcagg atgatagtct ctgcttgttt tttcaagtgc
     2041 ttctaaagta ttttgcgccc cattttgggt actcacacct ctgtgagaga cagtcactgg
     2101 ttttgtctga acagctgaag tcagataatc catattgtaa agacagacgc caataccgaa
     2161 aaaaactgca aaaagagtca aaccagtgtt tttcagcgca gagaaacgca agttgggggc
     2221 ttgtgggata aaccacttag ggatctcagg caagaagcct tcgtcatcca tgaaatcaac
     2281 aatgatataa aaaatcccta cggtagacat cacgatattg aataataaaa tcccttgtaa
     2341 aagcgtcata gcaaacacag cgctgatcaa tgcatagtct gggaaaaatt gttcaaccat
     2401 actttgtgcc aaaataacaa cgatatatcc taaagaagaa aggagtaaaa tcgtaccgcc
     2461 aatcacaata aattggcccg tgatggccaa aagacgcgat ttggtcaatg accagctttc
     2521 ccgaatggct gctcgaaaag gacgatcccg tagaatcatt tccggtaaag caaaaatcag
     2581 tcgaatccca atatagccaa ggaaaatata aacgagtaaa aaggaactga cgatgatcca
     2641 acgattagca aaaataaaat ccataatgaa agcggggatt tttatttttg acagtagatc
     2701 agaattaaaa cttaatccgc tgatcggtag aatcaataaa aaataagcaa ggaaaaacaa
     2761 aaaagtaatg ggtctcactt tcttcagctg aagtatcgtc aagtgtaaga gttgcttcaa
     2821 agagattggt tctttctttt taataaaaaa aacactcatc aaaagaaaag taaattcgaa
     2881 ataaactaac agtaaaatcg tcatcaaaac tacgattaac gagagtagaa ccccaggatg
     2941 ttgagaaaaa attgaaggaa tattgtcata agacaaatag tcaattgccc ctcggtgtaa
     3001 aataaaacgg gtcgtactgc tcaataaggg taaacaaata aataaaataa agccatgcat
     3061 cagcaagaca tcacggaaat aagcttgtgt tccttttaaa aagtcccaag cattacctaa
     3121 gctacgtttc aaataattca tcctatcacc tgctcttcac attatagcgc aaatctaatg
     3181 aaaaaggaaa gggtgcagtg tgttttgcag accctttccc agatttaaca ttgtcaatca
     3241 atgactaatt ttacaaaata aatattatcc aagaagacca cttagatatt gacctccata
     3301 aatcaaggcc atactatata agaaaatcga cagtggttta gcaatcaaaa tgattagtgt
     3361 atatcgtttc actgacatgt ttgtcaatcc agccatcaaa cataacgcat cgtcaggagc
     3421 aactggaaga aaaatagcta gagcaaacag cttctcaaaa cggttttgat tatccagcca
     3481 gccaatatat ttgttatatg tcttatcgct aatcatggaa agtatgaatg gctttccata
     3541 acgacgccct agcagaaaaa tgataatcga accaatgcag attccaatat agttatagat
     3601 aaatccagca taagggccaa atatcaggac accagctgca gtactgatcc ctccaggaat
     3661 gattggaata actacttgca ggatttggat gaagacaaaa ataatcggtc ctaaaatgat
     3721 ggaatcacct actaatcctc ttaacgcatt caaatctttg aaaacaccta aattaataaa
     3781 ataaatcgtc agcgcgattg ataggcctaa tccgataatg gatatgaaat ttatcagttt
     3841 tcgagaggca gcaatatt
//
LOCUS       DACDEG010000145         3801 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.150, whole genome shotgun sequence.
ACCESSION   DACDEG010000145 DACDEG010000000
VERSION     DACDEG010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.150"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(196..744)
                     /gene="aac(6')"
                     /locus_tag="IV142_002634"
     CDS             complement(196..744)
                     /gene="aac(6')"
                     /locus_tag="IV142_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP8486221.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            1297..2400
                     /locus_tag="IV142_002635"
     CDS             1297..2400
                     /locus_tag="IV142_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8486222.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            complement(2593..3660)
                     /gene="rlmN"
                     /locus_tag="IV142_002636"
     CDS             complement(2593..3660)
                     /gene="rlmN"
                     /locus_tag="IV142_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP8486223.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
BASE COUNT         1154 a          764 c          641 g         1242 t
ORIGIN      
        1 aatgtagaaa gccattttca caaaattttt cttactactc gaaactaagc gactttttca
       61 caacctctgg tacacggtgt tccaaaagta gcttcttcgg aaataagttg gaatttcaca
      121 aaaattcaag aagtaatttt cgtaaattcc ttcttatttc ttgaagctga ttacttctgt
      181 cacaacctct ttttattatt gagaatttgg tcgaggaata atcgtttttg ccatccaaat
      241 atccggtttg tcccagccat ttgcatttgg taagacacct acgattttat aacctaattt
      301 ttcatagaat tcatacggat gttcacgaag gttctggatg gaagccactt tatcaaatgt
      361 atgctcatac aggtccgttt gacttaacgt tgttccatgg tctaaatcat ccgtacctaa
      421 ataaatcgtg attcctcctc tggaagctac ttctttttct aagtaattga ctaatcgagt
      481 acctatttgg ttctttcgtc gggagctttc tacaactaat ggatgcaatt cccaacctgt
      541 gataccgtat tgagggattg caccaataaa tcctactaac tcatcttggt ctaccgctgc
      601 taccgcgatt cgttctggat tcatcatttc ttctacttct tctgccgagc tgtctccata
      661 ttcttccggc caagtcagtc tcagtaaatc agaaagctga tctttcaata ctggattatt
      721 acggtcaaat tcactgatta tcatacgtct tcccccttgt tcttgcttat tctaacagtc
      781 cttttgaaaa aacgaaatgt tctcgctcaa aaatctttag ctaccaatca gtatctctat
      841 tctttcatta tttatcttac ttataaaata aaaaaattat acagaaacac tttattcaaa
      901 ataggaaaga tcaggaaagc catcaattgg aggaaaaaac tctgtttgtt catcatgata
      961 tagattatct tttgctttaa aaaaaaccaa ttatgaattg acaaataaaa cctgttttca
     1021 tataataatc ttaattttta gaatttttag ataattacaa aaaagcaaag ggaatcggta
     1081 gaaatactaa gaggaacaaa aattcattat tgtcgttttc aatgtttttg tacttgttcg
     1141 tttttaggat acttattgat gatttcaagg tgttcatgcc accttattca tcacttatcc
     1201 tgtcttaggt tcacctctac tccatctaca gccgcagtca gatcgatcac tctttaaaat
     1261 tttaacccgt ttcaacgaag aaagaaggaa atgaatatga gtcaaagtgt tttcttacca
     1321 aattatacca ttggaacaga tgcgtatgaa aaagcaaatg gaatcgtcaa agaatatggg
     1381 acaaaagtag tgctgattgg agggaagact gcactttcaa aggcagaaaa caaaataaaa
     1441 gtagctttga cacaggaaat agaggttgtc gccagtctct ggtacggggg taattcaact
     1501 tatggaaata ttgaacagtt gatccagcat gaaagtgtcc aaaaagcaga cgtcctgttt
     1561 gcagtcggtg gtggccgtgc ttgcgatact gtaaaagtca ttggtgaaaa actagctaaa
     1621 ccagtcttca cctttcccac tattggttcg aactgttcgc ccgtcactgc ggtatgtgtc
     1681 atctataacg aagaaggcgt aatggacgga ctcttctttc cttcaaaacc acctgtacac
     1741 accttcattg atacagcaat tattgccgaa gcacctgata aatacttatg ggcaggtatt
     1801 ggcgatgctt tatccaaaga agtcgaatct actttttctg ctcgtggcga tgatctaggc
     1861 tacacggata gattaggaat ccaaatcgga aaaatgtgca ttgatcgtct cttgacttat
     1921 ggtacgcaag cattgaaaga tagtgccgac gatcgtccct catatgcaat tgaacaagtc
     1981 gtttcggata tcattgttac taccggattg gtttctgttt tagttaacaa taactataat
     2041 tctgctatgg cacactcatt ctattacggt acgactgttc tagaaaaaat ggagggatac
     2101 cttcacggag catctgtcag ttatggtgta ctgaccttat tgatgatgga tcagcagttc
     2161 gatttgtttg ataaaatcta tgtgtttatg aaaaagaacc aacttccagt atcgttaacc
     2221 gacatgaatc tcaatgtcac gaccgattta gaagcaatct tagataaagc catggcaaca
     2281 aatgatatta aacatgtgcc ttatgaaatc acccgagaaa aatttaaaca ggccattctt
     2341 gacgtagaaa cttatcatgc tacacaagaa agccagcaag gatataagaa aatcagttaa
     2401 ataattgcta aaacccccat caagcaggct gtcatcaaat tgaggacatt tctttgcttg
     2461 atgagggttt ttcttgtcaa aaaaagcttt atagctttcg ctatagaatc ttttctctta
     2521 tttaagttga acagttcctt aattaagaaa actgctcctt ttatggtaat tgaaaaaacg
     2581 agtaggatac gattatttgg caactgtttt tttcatttgc ttgcttcgca attgtccgca
     2641 agctgcatca atatctgtac catgttcttt acgtatcaca cagtttacgc cgtttttctt
     2701 caatacatca taaaatctca atacatcagc ttttggactg cgagaatatt gatcatgttc
     2761 actgactgga ttatatggaa tcaagttgac atatgctaat tttttcttat cttttaatag
     2821 atctgctaat tgttgcgcat gctctggtct atcatttact tgattcaaca tgatatattc
     2881 aaacgtgatt cgacggtttg ttttctctaa atactcatcc acagcttcca tcaatttctc
     2941 aattgggaaa cttcggttga tccgcatgat cgatgttcgc acctcattat tcggcgcgtg
     3001 caaggaaata gccagattga cttgcaagcc gttattagcg aattccttga ttttgtgtgc
     3061 taatccacta gtggaaactg taatgtgccg agcaccaatt gccaatcctt tggcatcatt
     3121 gatgatatgc aaaaagtcca ttacattgtc gtaattatca aatggctcac cgatccccat
     3181 tacaacgaca tgtgaaacgc gttcgccaag attgcgctca tcaaaataat gttggaccat
     3241 catgatctgt gcaacaattt ctccagcagt cagatcacgg ttctttttga gcagcccgct
     3301 tgcacaaaac gtacagccga tattacatcc tacttgtgtc gtcacacaaa ctgacaagcc
     3361 atattcttgt ctcatcagta cagtctcaat catgttttta tcagggagtt caaacaaata
     3421 ttttactgta ccatccgaag cttcttggac gaccatctgt tttagaggat tgatgataaa
     3481 atgctccgat aatttttcga tcaattgttt cgatagattc gtcatttctg tgaaattact
     3541 tacacgtttt tgatacaacc attcccaaac ttgggcagct cggaattttt tctcgccgtt
     3601 ttctataaac caattgatta attcttcatt cgtcaagcca taaatggact gtttttccaa
     3661 agtattttcc ctctttttct caaagtttcg ctacatacta tacatgattc atttccttaa
     3721 tgcaatactt cacaaataaa tcgaatagaa ctttaatctc acaaaaacta aaaagacaag
     3781 acatcatacc gtattagaaa a
//
LOCUS       DACDEG010000146         3801 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.151, whole genome shotgun sequence.
ACCESSION   DACDEG010000146 DACDEG010000000
VERSION     DACDEG010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.151"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            2..550
                     /gene="hslV"
                     /locus_tag="IV142_002637"
     CDS             2..550
                     /gene="hslV"
                     /locus_tag="IV142_002637"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP8486224.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            564..1964
                     /gene="hslU"
                     /locus_tag="IV142_002638"
     CDS             564..1964
                     /gene="hslU"
                     /locus_tag="IV142_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP8486225.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1982..2773
                     /gene="codY"
                     /locus_tag="IV142_002639"
     CDS             1982..2773
                     /gene="codY"
                     /locus_tag="IV142_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP8486226.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2855..3730
                     /locus_tag="IV142_002640"
     CDS             2855..3730
                     /locus_tag="IV142_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP8486227.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
BASE COUNT         1301 a          661 c          853 g          986 t
ORIGIN      
        1 catggtcgaa tcaccatttc attcaaccac tatctgtgcc gtcgaaaaag acgggaaatt
       61 tgctatggct ggagatggcc aagtaacaat gggtgaatct gttgtcatga aaggcactgc
      121 aaagaaagta cgacgtatct acaatggaga agttgttgta ggtttcgccg gaagtgttgc
      181 agatgcgttt actttagaag aaaaatttga aggcaaacta aacgaatata acgggaatct
      241 acaacgcgca gctgtcgaat tagctcaaga atggcggaca cagcaatcta tgcaaaaact
      301 tgaagcgatg ctgatcgtaa tgaatgataa agaaatgctt cttgtttcag gtacaggaga
      361 agttatcaca ccagatgatg gtatcttagc tatcggatca ggagggaatt ttgcgttggc
      421 tgcagcccga gcaatgaaaa actatgctca aaacgacatg agtgcgaaag aaattgcaga
      481 aaatgcgtta aatattgccg cagatatctg tatctttact aatcataata ttatcgtaga
      541 agaactgtaa gaggtgagta gatatgtatg aattaaaaca aacaccaaga caaatcgtaa
      601 gtgaattaga tgaatatatc gtaggacagc acgcagcaaa aaaatctgtt gccgttgctt
      661 tgcgtaaccg ctatcgccgt ttgcagttag atgaaaaaat gcaacaagat attacaccaa
      721 aaaatatgct gatgatcgga cctacagggg tcggtaaaac agaaatcgca cgacgtttgg
      781 caaaaatcgt caatgcacca tttgtcaaag tcgaagcaac aaaatttact gaagtcggtt
      841 atgtcggaag agatgtagaa tcaatggttc gggatttagt agaaaatgcg atccaaatcg
      901 tagaaaaaga gcagtacagc cgtgtataca caagtgctaa aaaaaatgca gaaaaacgtt
      961 tggtaaaaat cttggtacct ggtatcaaaa aagaacagaa acaatccact aatcaatttg
     1021 aacaaatgat gaatcttttt aatgctagtc agcaaaatac cgctgaaccg caagaagaag
     1081 tgacagaaga tattcgaatc aatcgccgta cagtcttgga acaactacaa aagggtttac
     1141 tggatagtcg tgaagtaacc attgaagttg aagaccaaaa gaaacctgca ccaatgatga
     1201 acaatggatt ggagcaaatg gggatcgacc taaatgaaac tctaggtgct ttgacaccga
     1261 agaaaaaagt ggaacggacg gtaacagtca aagaagcttt agagttattg atcaaggaag
     1321 aatcagcaaa attagtcaag gatgctgata tccatagtga ggcaatcaaa ttagcggaag
     1381 aaagtggaat tatttttatc gatgaaatcg ataagatcac ttctaaatca cagcaatctg
     1441 gagaagtatc cagagaaggc gtccaaagag acatattgcc aattgtcgaa ggttcacaag
     1501 taaacacaaa atacgggatc atccaaacag atcacgtcct attcattgct tcaggagctt
     1561 tccatttgtc taaaccaagt gatctaattc cagaacttca aggacgcttc ccgatacggg
     1621 tagaattaga tgatttgaca gctgaagatt ttgtgaagat tctgaccgaa ccaaataatg
     1681 ctttagtaaa acaatacgtc gcgttgatcg gtacagaaaa tgtgtcagtt acgtttacaa
     1741 aagaagcaat tgaacggatc gctcaaatcg ctttcgatgt caaccgtgac acagataata
     1801 tcggcgcacg cagactgcat accattttgg aaaaactact agaagatcta ttatttgaag
     1861 caccagatat gcaaatggga gaaataacga tcacagaagc atatgtggat gacaaacttg
     1921 gctcaattgc ggcaaatgaa gatttaagtc gctatatttt ataaaaaatc ggaggacgac
     1981 aatgacagac ttgctaacga aaacccgcaa gataaacaca cttttgcagc aaaagaatac
     2041 ttttgacatg caagcagatt tgccttacga taaaatggct gtcacactag gtggtattct
     2101 tgacagcaac acctatatca taggaagtga cggagtatta ctaggttata atgaacgtca
     2161 cgatgtgaac aatgaacgaa tcaaaacaat gtttgttgag aaacaatttc ctgaatctta
     2221 tactgataca gtggcacagt tgatgaaaac tgaagctaat atcccaatta ctagtgatat
     2281 tactgcattt ccggtagagt caagagagaa atatccattt ggtctgacga ctgtcgttcc
     2341 gatctttgga gctggcgaac gtttgggtac gattatctta tctcgtatgg aacaatcgtt
     2401 tgcagatgac gatctagtct tagcagaata tggagctacg gttgtcggta tgcagatcct
     2461 ttatcagaag tctcgaagca tcgaagcaga tgtacggagt gcaacggctg tccaaatggc
     2521 aatcaacaca ttatcttata gcgaattgaa ggctgtacaa gctatctttg aagcattgga
     2581 tggagatgaa ggtcgtctga ctgcttctaa tattgccgat agtatcggca tcactcgttc
     2641 tgtgatcgtc aatgctttac gaaaattgga atcagctgga atcattgaat ctcgttcgtt
     2701 aggcatgaag ggaacttatt taaaagtatt gaattcccgt tttaaagacg agttagcaaa
     2761 acacagttat tagaagtaag gggactgggg cagatagaaa tgtttgttcc agccttccca
     2821 ttttaaaaag taagtcgaag ttggaggaat ggcaatgact gttattattc aaaatgacca
     2881 gctgatcgcc gaaatcgctg aacacggtgc ggaattgatc agtctgaaat caaaagaaca
     2941 tggtttagag tatatctggc aaggtgatcc agcgtattgg ggcagacatg caccggtttt
     3001 attccctttt gttggacgtt taaaagatga tcagtatgtt tatcaaggga aaacatatca
     3061 aatggggcaa cacggatttg ctcgggatat ggattttgaa gtcattgaac atggacaaga
     3121 aatggctagt ttcatcttaa ggagtacgcc ggagacatta gaaaagtatc catttgattt
     3181 tgaattagtg atcagctatg aattaggagg agacggcatc acggttcatt atcaagtggc
     3241 aaataccggt gaaaaagaaa tgttcttttc tatcggtgga catcctgctt tcaatgtacc
     3301 tcttgaagaa ggattgtcat ttgaagattt ctatctggca ttttctccat tgaaatcaag
     3361 attacgccta ccattgaaag gcccattcat cgatatggat caaaagacgt taggtcagac
     3421 aaatacgaat ctggctttga atcgtgaact ttttgagcaa gatgccttgg tttttgaaac
     3481 aaggggtcta aatgcgtacg ccattcgaag tgaaaaatct tcccatagcg taactttaag
     3541 ctataaagat atgccttatg tagggatctg gtctccttat ccaaaagaag cgcctttcgt
     3601 ttgtatcgaa ccgtggtgcg gaatcgctga cacggtgaac agtagtggaa acttgatgga
     3661 aaaagtaggg atcaatcaac taggtgccca tgaaatcttc aagacaaaat attccatcac
     3721 agtgaagtaa gaggttgtga ccgagaagtt agcagggaac accgtttacc aaggtgtgta
     3781 aaaaagctat cctgtaagta a
//
LOCUS       DACDEG010000147         3797 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.152, whole genome shotgun sequence.
ACCESSION   DACDEG010000147 DACDEG010000000
VERSION     DACDEG010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.152"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1110
                     /locus_tag="IV142_002641"
     CDS             <1..1110
                     /locus_tag="IV142_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8486228.1"
                     /translation="EVDKIDIQNKKISCVKKDGTRFSQEYDKVILATGSKQIMLDIPG
                     NNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYIGVEIAEAIRKRGKEVYLFDV
                     ADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYLGRKKIEKIVTTSGTQYSMDM
                     VVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKDIYAIGDCATTFFTSINRSSV
                     DFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFNYNFYAAGLNLKTARLLSESI
                     DYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQLCSQEDLSGLLSMFSLAIEEQ
                     ITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            1222..2673
                     /locus_tag="IV142_002642"
     CDS             1222..2673
                     /locus_tag="IV142_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8486229.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            3022..3654
                     /locus_tag="IV142_002643"
     CDS             3022..3654
                     /locus_tag="IV142_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP8486230.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
BASE COUNT         1442 a          520 c          706 g         1129 t
ORIGIN      
        1 gaagttgaca aaattgatat tcaaaataaa aagattagtt gtgtaaaaaa agatggtaca
       61 agattctctc aagaatatga caaagttata ttagctacag gttcaaagca aattatgcta
      121 gatataccgg ggaataattt agctaatatt ttttcgataa agaattttcc aaatgcaaaa
      181 gagattttta aagctttgga tgatagtact attaaaaaag taggaattat tggagcagga
      241 tatattggag ttgaaattgc tgaagccata agaaaaagag ggaaagaagt atatcttttc
      301 gatgttgcag atagagtgtt gtcgacttat tacgaccgta gtttttcaga taaagttgaa
      361 gaaatactat caaaaaatgg aatacatctt tgtctatcag aaagtcctaa aaaatattta
      421 ggaagaaaaa agattgaaaa aatagtcact acaagtggta cccaatactc tatggatatg
      481 gtcgtacaag ctataggatt tctatctaat agtccaattg gggaaaagga actcttgaga
      541 gatgttagtg gagcatatct aactaatgaa taccagcaaa caaatataaa agatatatat
      601 gctattgggg attgtgctac aacttttttt acgtccatta atagaagtag tgttgatttt
      661 tctatttcta atgctctaag aacagcttat attgcaactc aacatattaa caatcaagat
      721 tttacacctt ctggttccca atttacaaat ggcttttcta tatttaatta caatttttac
      781 gcagcaggtt tgaatttaaa aacagcacga ttattaagtg agtctataga ttatatagaa
      841 atagaagatt tcattagacc tgcttttttg agaaaaaata gtcgtgtaaa attaagaatt
      901 agctttgaaa aaaatacaaa gagaatagta ggtgtacaac tttgtagtca agaagattta
      961 tctggactat tatcaatgtt ttctcttgct atagaagaac aaattacatt agacaaattg
     1021 aaaatgttag attatttatt tcatcctagt tttagccaac cctataattt tattttaaag
     1081 gcagtaacag gattaaacat tgaaacataa tcgttactaa atgaaatcaa gaaatatact
     1141 gtattactca attcattgaa aatatttatc ataattttta atagaaaaca ctattaaaga
     1201 gaaaaaagag aggtaaatca aatgaaaaaa tctttaaaac ttgcatcagc agttatatta
     1261 atgtcccaac tccttatagc aacacctttc catgtgttgg caagcgaaaa ttcaacatac
     1321 aaaattgcaa aagaaagaac cgagacgact gaaatgagta attccgatgt aacagcagag
     1381 agtagtgaac ttttgacaac ttcatcaagt gaaagtagta cgacttcaaa tgccgagtct
     1441 tcaacaactt catcaagtga aagtagtacg acttcaaatg ccgagtcttc aacaacttca
     1501 tcaagtgaaa gtagtacgac ttcaaatgcc gagtcttcaa caacttcgtc aagtgaaagt
     1561 agtacgattt caagtaccga atcttcaaca acttcaagta gtacaagtga aataacttct
     1621 tcgaaagtga caaaaccaaa ccatatagaa tcttcaaaaa aggaagcgcc aaaaacggaa
     1681 ggacaaacaa aaactttttc gacaccacag caaatagaat caacagaatc tttccaatca
     1741 caaccaatac tatctggtaa tgaagaaatt cattttgaca aaaatgaatc tgtagaaaat
     1801 tttattatga aaattggtga gtctgcgaga aaaattggtc agaaatatga tttatatgct
     1861 tctgttatga ttgcacaagc gatcttagaa tctgccagtg gtcaaagcca gttagctcag
     1921 gcaccgaatt acaatttatt tgggataaaa ggaacttata atggaaactt tgtcattatg
     1981 gtaacaaacg aagatttggg taatggaacg ctttatacga cccaatcaaa atttagagtg
     2041 tatgaaaact atgaggaaag ctttgaagat tatgccaaat tattaactaa gggaattagt
     2101 ggaaataaag atttttatgc aggagcatta aaagctaata gtaaaacata tcgagaagca
     2161 actaaattcc ttacaggacg gtatgcaaca gatacacaat actatttgaa gctaaatgaa
     2221 ttaattaaaa cctacgattt aaccaactat gataaagaag ttaaaggtcc acaaatgagt
     2281 aagactggat accaagtgcc tattcataat tattcaataa ccagtttgtt tggaaatcgt
     2341 ggaaatgaat ttcaccgagg acttgatatg gcagctacac agggtgagcc gattcatgca
     2401 gttaaagctg gtaaggttgt taaagcagag tttcactact cttggggaaa ttacgtggtg
     2461 atacaacacg aagatggatc aaccgcgtta tatgctcacc agcaacaata cctagtaaaa
     2521 actggagata atgttttgca aggacaaata attggctatg taggaagtac tgggaatagt
     2581 actggcccac accttcattt ggaaatttgc aaagatagta gtctttctca gagcatgtta
     2641 gttgatccga aaaccttatt attcggagac taaaaaaggt aatcaatctg acaaagtaac
     2701 aagaaaatgg ttggattctt agacctttga tcaggatatt gagactggaa ttgatatctc
     2761 gattacgatg ggtttgacaa ataaaacaag tccattatcg aatatcgagt gtattattgc
     2821 cgtctttatg ctcatatttt gagcaggttt cactagatga aaacaattta tctatcttga
     2881 ggatttcgtg tacgtatcgt tcagtcttgt attgtaaatt agtcataaaa tcagactaag
     2941 aaaaattgga aatatttttt tctgatttat taaaagttaa ttgaaattat atggtacttc
     3001 tttcttggga gggtaggtgc agtgaaggaa agatatggtc accttactgt tgttgaggag
     3061 attactcata atggatataa aaaatatcgt tgcctatgtg actgtgggaa tgaaacgatt
     3121 gtatatcgat caaacttact atcaggtaga accaaaagtt gtggatgtgg aaattataaa
     3181 aataggcata aatataaaaa tttgacaaat aaaacttttg gtaagttagt tgcaatcaag
     3241 ccaactaaac tgcgaagtga aggaactgtt gtttgggaat gtcaatgtct gtgtgggaga
     3301 aaaaagttag tatcggcaaa gcgacttcta aaaggtgatg taaaaagctg tggatgtttg
     3361 aaatcaaaaa agcatcacat tgaaaataaa aaattcggat tacttactgc aaagtatcct
     3421 ttggacaata actgttcatg gaaaacaaaa tgggtttgtc attgtgagtg tggcagtatg
     3481 tgtgagataa gttatagtaa cttatttttt ggacatactc aaagttgtgg atgtttaaaa
     3541 aggcaagaaa caaggacatg tgttgaaggg acagtaatag agtgtctaac gtcaaaatta
     3601 ccgaaaaata atacaagtgg tgttaaggga gtttatcgtt ctaaagagaa atagattgcg
     3661 tatataactt taaaaaaaaa gaggtactat ttaggatcgt atgattctat taagagtgca
     3721 gctcaagcta gaaaagatgc tgaaaataaa ttgtttcaac ctttgattga caaatatcga
     3781 ggtaatcatt aattatt
//
LOCUS       DACDEG010000148         3729 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.153, whole genome shotgun sequence.
ACCESSION   DACDEG010000148 DACDEG010000000
VERSION     DACDEG010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.153"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1244
                     /locus_tag="IV142_002644"
     CDS             <1..1244
                     /locus_tag="IV142_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP8486231.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
     gene            complement(1321..1758)
                     /locus_tag="IV142_002645"
                     /pseudo
     CDS             complement(1321..1758)
                     /locus_tag="IV142_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318498.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            2072..2494
                     /locus_tag="IV142_002646"
     CDS             2072..2494
                     /locus_tag="IV142_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486232.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            2504..2707
                     /locus_tag="IV142_002647"
     CDS             2504..2707
                     /locus_tag="IV142_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486233.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            2918..3502
                     /locus_tag="IV142_002648"
     CDS             2918..3502
                     /locus_tag="IV142_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8486234.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         1233 a          603 c          719 g         1174 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgtacaggta ctgttctatg gggcgcttac
     1321 tttataaact ccatttgctt tcgcaatttt aatgccttgc gcctggcgtc gtttagattc
     1381 ggtacgctct tgttcagcga tcatggccaa aatttggatg atcagatcct taatgaattt
     1441 atccaggaga gggttgccaa ttgcttcagc catgatcggt agactggtga tgattagctg
     1501 tacgtttttc ttctttaggt aattgactga agcgatgatc tcgtcgtaat ttcttccaag
     1561 tcgatcaata gcttccacaa taaaaatgtc ttgatctcgg acgaaatcca atgcttcttg
     1621 gaaaacaggt cgatgagtaa tcgttgcgcc cgattgtttc tcgatgaaaa ttctcactgc
     1681 accgaatttt ttcatttctt caatctggcg ttgttcattt tgatcgatgg atgagacacg
     1741 tatgtatgca actctcaaaa tttttaccta ctttctgcaa ctatttaaaa tacaccctaa
     1801 tcatacacga taaaaccaga tttttcaatg ttgtggtcgt gtctagttag ggtatacctt
     1861 atatagacaa aaactaatga tggaggaaat agtatgtgtc acttttcttt ttaaatggca
     1921 atattttttg taaataaatg tcaatcaaga aaatttaaac gattatgaca ttaaaaatat
     1981 attttatgaa ttttctagta tataacttga tttatctgtt aagatttatt aagaaaacgt
     2041 tttcttattt ttgatttcga ggtgatagac aatgaaaaat aagtttattt tatctttagg
     2101 tattttaact attggaatta gtgtaactgg aactacaatt cctacatttg ctgatagtac
     2161 aaatgaacca actactgtag tagataataa ccaagaatta actcctttag ataggaacgg
     2221 aatagccatt ggtaacccaa ctaacgactt aactgttact gaaaattatt taatagtttc
     2281 tccattactt gctcgagcag cttctaaaac gatacgagtg acaggaacga tgatttttga
     2341 taaagttggt tggccttcta gacctcctca atcacggaat tggaaagaaa aaagaaatgg
     2401 ttattggtac tatggaaaat tgtatttaca aagttataaa tcagctggaa acaattggat
     2461 tgcgacttat tcaggaacct taactagaaa gtagggattt tttatgccag aatttgtgaa
     2521 cacaccaaat ttttatcaag atagcgatat tctaagtaaa attaatttac cgaaagaaga
     2581 atcagcttta caagttcagc gaactttaca ggagacacaa aaaattcaaa aatcaatttc
     2641 taatctagaa gattctgaag aaaaaagaaa aaaaagagac caaaaaacat atcaaaaaga
     2701 aaaataatga ttttaaaaaa agagtgtcta gtcgacattc tttttttcta attgaaagtg
     2761 ttcataaaat cacccatttc tggacacttc ctttttttgc tttatttttc aaaaaaacaa
     2821 ttcatttcat gttcataaac ccataacaat atctatgttc agttaaacta tatggataaa
     2881 gttatgttac aataaaatta atgaaggagt gatttttttg aaaataggat acgcccgggt
     2941 atctactggt ttacaaaatt tggatttaca agaagacagc ctaaataaat ttggttgtga
     3001 aaaaattttt actgatcata tgagtggggc aaaaagtaat cgaccaggat tagaaatggc
     3061 cattgatttt gttcgctctg gcgatacact tgtcgtttgg cgcttagatc gtttaggtag
     3121 aaatatggaa gatttgattt caatcgtcaa tcgactgaat gagcgtggtg tgagttttca
     3181 tagtattcaa gaaaatatta cgatggacaa atcaagttct acaggacagc tcatgtttca
     3241 tttgtttgcg gcatttgcag aatttgagcg taatttgatc ctggaacgat ccgcagcagg
     3301 acgagcagct gctcgtgcaa gaggacggtt tggtggtcgt cctgaaaaat tgtctgaaca
     3361 agatttggaa ttactgaaga ctttagtgga tagcggtaca cctattaaga ctattgcgga
     3421 acggtggaac gtctcaagaa ccaccattta tcgttatttg gcaaaaatgg aggagaaaaa
     3481 tggaaagaca aattctcatt agtttttgtc atttaatgct gaaaattttt gccatttatc
     3541 tagaaaagtg acaatagtat gagaattttt tgtaagtgtc tactgataac ttattatcaa
     3601 atgttgtaag gattttattc atttgttaat attaaagaac ggaactccaa aaaagtccgt
     3661 actctaattt gcagtctctc actcttttag agtgagagtt tttattagat tttcaaatta
     3721 attagtaaa
//
LOCUS       DACDEG010000149         3692 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.154, whole genome shotgun sequence.
ACCESSION   DACDEG010000149 DACDEG010000000
VERSION     DACDEG010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.154"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..504
                     /locus_tag="IV142_002649"
     CDS             <1..504
                     /locus_tag="IV142_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8486235.1"
                     /translation="SEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLTYKEGSAQ
                     VIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAKTPLQSEK
                     NIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLLLVLATFS
                     YAVLRYI"
     gene            531..995
                     /locus_tag="IV142_002650"
     CDS             531..995
                     /locus_tag="IV142_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486236.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            992..1234
                     /locus_tag="IV142_002651"
     CDS             992..1234
                     /locus_tag="IV142_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486237.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            1252..1554
                     /locus_tag="IV142_002652"
     CDS             1252..1554
                     /locus_tag="IV142_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486238.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            1624..3687
                     /locus_tag="IV142_002653"
     CDS             1624..3687
                     /locus_tag="IV142_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486239.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVA
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKYEKIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEE
                     AQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
BASE COUNT         1452 a          560 c          763 g          917 t
ORIGIN      
        1 tctgaagtac aatatctagt cacacaaaaa tggcacacaa aaggcgtaga cgctgcgtca
       61 gatcactaca aacaatttag tattcaagat ccaattgaag ctcgtttgac atataaagaa
      121 ggttctgcgc aagtaataga taagagtaaa ggaaaagata ttacttctga aggcacacta
      181 acctatgata gcaattcaag aacgctgaag tgggaagctt ccgctgactt cttaagtaac
      241 aatcttttag atggacgaga aattcagctg atatttacag ctaaaactcc attacaatca
      301 gaaaaaaata ttgataacca agccgtagta gcagtagata atgtttctaa taaaacaaat
      361 gttgtgacaa ttggggttga tcctaacttg ccacaggtca ttgttcctaa aacaggttct
      421 acacatttag taacgatttc agcggtcagc ttactattac ttgtgttagc aacttttagt
      481 tatgcggttc tgagatacat ttaaaataaa aaaacgaaag aatgaataaa atgaaaaagt
      541 taataatagt agttacttcc ttgattgcca tgattgttat tttagtcgga tgtagtaatg
      601 aaaaaaataa acaaactaat caagataatg gcgtattgaa atcaggaact atgtggaaag
      661 aagaagtagg tggtcttgtt tataatttga aaattataga cgagactacc tgggaatact
      721 ctgagagtgt atggcatcca gatcctgtac aaataacagt caagaggcaa aaagactata
      781 aaggtttaga aagatacaaa atagtagatt cagctggtgt tagggaattt ataaacaaat
      841 ctgattcttt atttattgtt gttccctatg agaagaatgg cgtaaaaaaa ataatttttc
      901 ttggatctag caaggatgaa aagcgaacga aagagaaatt gattcacgat ggatcacaat
      961 ccaataaata taagttacaa aagactagcg agtgattgct agtcttttta tttgtcctga
     1021 aggaggaatc acagtggcaa aagctaaaac gccatttgaa aaagccgcgt ctaaaatctt
     1081 agaacgccgc ggtgtatctg aaacacagtg gaaacaagaa gtccttgaaa aagcacaatt
     1141 tgacctatta cggggaaaag acaaggactt agaaggatat atctatcaac gtgaacgtga
     1201 aaagcttgtt ttcgatgaaa ttattaaaca ctaaaaggag cgaacaattt tatgtttgaa
     1261 tcaattacaa tgaatttttt aacacaaccg aaagtagtag ctgcggttga tatttttaga
     1321 gcattacaaa ctgccgcaac tagtttaaaa aacaatctga tgatttttgc tgctgctgcg
     1381 gccgttgctt ccattgttat tgggggtctt ttcttcctat ttggacgaag aggagctgaa
     1441 aatgggaaga tgcacgtagg ctatgtattg atgggggtag gagttatttt agcagcagct
     1501 tctattgtgg tttcgttatt tggattgttt ggacaaacac ctcaaggatt ataagaaagt
     1561 aaaaaagtag gagtaagaag tagtcgatac tttttactcc ttttttatga aaggagaata
     1621 ctcatgtact ttacgattgc aggcaatacc aaaacagata aagagttaat aaaaaaagca
     1681 acgaaggaag taggaaagcg tacttctttt ttaccgaaaa caattaaata ttctgtcatt
     1741 gaaacggaag aacagctaca tgtgttaggg aaagtactgg aacacgaaag tcgatcttct
     1801 gttaagttga ctctcacgtt acatgacaca gaaggaaaaa tagagaaagc tggtacagaa
     1861 tacgtttttg gcccactgga ttatcaaatt tcagaaggtt tctatcctac attgatggat
     1921 ttaatccgcc ttgatgtgtt taaagaaacg gatgaaacta tgagtgtgga agagtcttat
     1981 cgaaacgaaa aacttttaca agttttagaa gaagagttag agagcgacca aagaaaagtg
     2041 aatcaaggga gtgaagtcga agtaaatgaa gaagtaccat gggaacgacc aatatttcca
     2101 aagaaggagt caaaagaaaa agagacagtc tatgttgacg aacaagctga tattccgact
     2161 cagaaatcga tatttgagga agagaacgat gtgatgggtc aattggaggt agatcccttt
     2221 gaagaaacaa tggaggatga agagacggcc gaagtagaag ttgatccagt tgaagcgaat
     2281 gaagagtcaa catggactga aggggattct caggaatata ttgaaccact tatcagtgaa
     2341 gaagtagaaa aaaacgaaat agagatacaa aaggcaactg tatttgatta tattttggat
     2401 caatatccgc cagagcatga atggatgaaa gaaaaaaaca aattattttt aaatgagctt
     2461 tattcgaaat atcaattacc ccaaacctat caagcatttt tagagagtaa agaagaatta
     2521 atctcacagg gtaaaggcac acttttagaa cagttagagt ctgtccaatc aacaaattgg
     2581 catgagcttg cacaagaaga tttattagcc acctttgaag aacgtcaaca agaaacagat
     2641 caagaaatta atgagtatgc gacggaacag tcgtctaatt gggagaaagc aaaagaggaa
     2701 ctaaataaag aagaacagcg agtgattgaa gaagaagtac aaaaaattaa agagcgatac
     2761 gaaaagaaac gcgacaatgc gtattccttg tgtgtagcaa agatcaataa ttttactcag
     2821 acgaatcaag aagcgttatc gaaagaaaaa gaaaacttgt taaaagaacg tattaaagaa
     2881 cgaaaagaag aattttaccg aggtttgcgt acagataagc taaagattag caaagaatta
     2941 aatgaacaac tgaataagct atatcaaaca acgactgaag ttcttatgga aaaatacgaa
     3001 aagatccagc aagaattaca agtgcaaatt ccaaaatgga aaaaagagta tgaaattcaa
     3061 caacaagaat tgaaagaaca agaagaaaaa caacggcaaa aagaaaaaga gcgcgaacaa
     3121 ttagaactaa aaaaacgtga acaggatctt gcggaacaac aaatggcaat agaaaaacaa
     3181 cggttaaacc aagaaaagga aaaagaagca gctgctcgta aggaacgtca agaacaaatg
     3241 gatacattgc gacaagctca gttaaattat ctagcacaac ctcaaccaac gccttcagta
     3301 gctgctgttc aaaccccaat gaacaaccaa acaaatctaa ctccttcacc acagcgttcc
     3361 tttaacggat ggatggtggg atgtattgca agtttaagct tgcttctagg tggaggaaca
     3421 gtatttgctt ttaatcatat caatcaatct tcacaggctg attctgtggc cacaacgacg
     3481 tcaatcaagg aagaagccca agaacaagca agaaaagagt atgaacaaca attagaagaa
     3541 ttattgaatc gtaccaatca gtcaacaaca gaatcaagta tggaaaatag caaagagagt
     3601 gatcctagta aggctaaaga aggatcaagc gattcaactg tacaatcaaa cacttccgaa
     3661 agttcagaat caaatgaggt gaaataatgc gt
//
LOCUS       DACDEG010000150         3624 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.155, whole genome shotgun sequence.
ACCESSION   DACDEG010000150 DACDEG010000000
VERSION     DACDEG010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.155"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            746..829
                     /locus_tag="IV142_002654"
     CDS             746..829
                     /locus_tag="IV142_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP8486240.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            954..1691
                     /gene="erm(B)"
                     /locus_tag="IV142_002655"
     CDS             954..1691
                     /gene="erm(B)"
                     /locus_tag="IV142_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP8486241.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1954..2070)
                     /locus_tag="IV142_002656"
     CDS             complement(1954..2070)
                     /locus_tag="IV142_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta-toxin"
                     /protein_id="HAP8486242.1"
                     /translation="MVQNQHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI"
     gene            2333..2845
                     /locus_tag="IV142_002657"
                     /pseudo
     CDS             2333..2845
                     /locus_tag="IV142_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            2894..3574
                     /locus_tag="IV142_002658"
     CDS             2894..3574
                     /locus_tag="IV142_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8486243.1"
                     /translation="MNHFKGKQFQKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEYSKILYHLWKKKNRQSFYSWKMDETYIKIKGHWHYLYRAIDSDGLTLDI
                     WLRKKRDTQAAYAFLKRLHKQFGQPRVIVTDKAPSISSAFRKLQINGLYTKTEHRTVK
                     YLNNLIEQDHRPIKRRNKFYRSLRTASTTIKGMETIRGIYKKNRRNGTLFGFSVSTEI
                     KVLMGIPA"
BASE COUNT         1197 a          597 c          687 g         1143 t
ORIGIN      
        1 ttgacaacca tttttgttag gcgtttgagg aagtgaagct gttttcctca tgttttaaaa
       61 gctagaaata aattacagtg tacgctcctt tcttttttcg ttttgttggt tcattttcgc
      121 cagtgtgttc gtgtcgtttg ctgtgacgag tacgcaacat agacacaaag aaaacacaag
      181 ggcgagcttg ctcgtttata tacccttgtg ttttcgactg gatatgttaa ggtaacaaac
      241 agtaaatgac acgttacaga ctgcaaaatg taccaactga aaaaagaaag aataccgttg
      301 tgtgttttat ttctcgatca gcttttaaaa catggggtcc cgagcgccta cgaggaattt
      361 gtatcgataa gaaatagatt taaaaatttc gctgttattt tgtacattta acttgacggt
      421 gacatctctc tattgtgagt tattagtggt acagttttca accgttttaa ttataaaaaa
      481 gtggtgcatt tttaaattgg cacaaacagg taacggttat tgcaggtgta tttcttatct
      541 atgggtttaa catggatttt atcattaaaa tcatgagtat tgtccgagag tgattggtct
      601 tgcgtatggt taaccctaaa gttatggaaa taagacttag aagcaaactt aagagtgtgt
      661 tgatagtgca ttaaaaattt tgtataatag gaattgaagt taaattagat gctaaaaatt
      721 tgtaattaag aaggagggat tcgtcatgtt ggtattccaa atgcgttatg tagataaaac
      781 atctactgtt ttgaaacaga ctaaaaacag tgattacgca gataaataaa tacgttagat
      841 taattcctac cagtgactaa tcttatgact ttttaaacag ataactaaaa ttacaaacaa
      901 atcgtttaac ttctgtattt gtttatagat gtaatcactt caggagtgat tacatgaaca
      961 aaaatataaa atattctcaa aactttttaa cgagtgaaaa agtactcaac caaataataa
     1021 aacaattgaa tttaaaagaa accgataccg tttacgaaat tggaacaggt aaagggcatt
     1081 taacgacgaa actggctaaa ataagtaaac aggtaacgtc tattgaatta gacagtcatc
     1141 tattcaactt atcgtcagaa aaattaaaac tgaatactcg tgtcacttta attcaccaag
     1201 atattctaca gtttcaattc cctaacaaac agaggtataa aattgttggg agtattcctt
     1261 accatttaag cacacaaatt attaaaaaag tggtttttga aagccatgcg tctgacatct
     1321 atctgattgt tgaagaagga ttctacaagc gtaccttgga tattcaccga acactagggt
     1381 tgctcttgca cactcaagtc tcgattcagc aattgcttaa gctgccagcg gaatgctttc
     1441 atcctaaacc aaaagtaaac agtgtcttaa taaaacttac ccgccatacc acagatgttc
     1501 cagataaata ttggaagcta tatacgtact ttgtttcaaa atgggtcaat cgagaatatc
     1561 gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa acacgccaaa gtaaacaatt
     1621 taagtaccgt tacttatgag caagtattgt ctatttttaa tagttatcta ttatttaacg
     1681 ggaggaaata attctatgag tcgcttttgt aaatttggaa agttacacgt tactaaaggg
     1741 aatgtagata aattattagg tatagggcac ctctaataac taccaattaa ttcacctcaa
     1801 aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt tcttatcgat acaaattcct
     1861 cgtaggcgct cgggacccca acatgaggaa atgtttttat aaaaaaagag tagttaccgt
     1921 tttttggtaa ctactctttt ccttttttaa ggtttaaata cctggaagtt taggtgtttt
     1981 gggtattggt ggtgtaggtg gctgcaagct ttccaatttt tgttgaattg ctttaaattc
     2041 aggggtttct tggtgttgat tttgaaccat tttttgctct attctctcca aagtcggttg
     2101 aatttctttc cctgatactt tacgatttaa ttctctttct aaggtctctt tcggactaat
     2161 ggaaggcgtt tctaagcttg aatagagttt cactccttct ctattgtaaa gccttatatc
     2221 gctaaataag cccgttttat gaagggtttc taaattggtc ggtaagtttt tgacaacaat
     2281 atcatgcgct tgttttggtg gtgccctagc tgtcattgga tcatctgcat acaataagat
     2341 ccatgaaagg ccatctgctg ccaatgagcg ccttgagatt ggtaattggg aagctgatac
     2401 tgtagccggt aaaacaggta gggctcgtat tgtaactcta gtcgatcgta gatctcgttt
     2461 ctccctactt ggtaaggttg aaaagaaaat ctctaaaaat gtggttgatt gtatgatcaa
     2521 tctactaaga aatgtaggag aagataaatg taaaaccgtt acacccgata gaggaaaaga
     2581 attctcccaa catagtcgaa taacgctaga attaaatggc actaaagtgt attttccaga
     2641 tccacatgct ccatggcaac gtggtacaaa cgaaaatact aatggtttat tacgagaata
     2701 ttcacccaaa ggtgaagata tttcaggtat tttcgatagt caaattcaag aatgggcgaa
     2761 taaattaaac acgcgaccac ggaaatgtct aaattggaaa accccttatg aagtattttt
     2821 ggttctgttg caaagttccc cataactcct gatttattgt aaaatgtaag aaaacgagag
     2881 tgagggtaga cagatgaatc atttcaaagg gaaacaattt caaaaagatg tgattattgt
     2941 cgctgttggt tactacctgc gttacaatct gagctatcgt gaagttcagg aattactgta
     3001 tgatcgcgga ataaatgttt gtcacactac gatttatcgt tgggttcaag agtacagtaa
     3061 aatcctctat catctttgga agaagaaaaa tagacagtcc ttctactcgt ggaaaatgga
     3121 cgagacctat atcaaaataa aaggccattg gcattatctc tatcgtgcga ttgattctga
     3181 tggattaact ttagacattt ggttacgaaa gaaacgggat actcaggcgg cttatgcttt
     3241 cttaaaacgg ctccataaac aatttggaca acctagagtg attgtaaccg ataaagcacc
     3301 atcgattagc tctgcattca gaaagctaca gattaacggt ttatatacca agacagaaca
     3361 tcggacagtt aagtatctca ataatttaat tgagcaggac catcgtccaa ttaagcgccg
     3421 taataaattt tatcgaagtt tacgaactgc ctcaaccacg attaagggca tggagactat
     3481 tcgaggaata tacaaaaaga accgaagaaa cggaacgctc ttcggatttt cggtgtcgac
     3541 tgaaattaaa gttctgatgg ggatcccagc atagtcaaaa tattaaacta ggacttctat
     3601 actcttttgg ggaactttgc aaca
//
LOCUS       DACDEG010000151         3599 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.156, whole genome shotgun sequence.
ACCESSION   DACDEG010000151 DACDEG010000000
VERSION     DACDEG010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.156"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            19..2016
                     /locus_tag="IV142_002659"
     CDS             19..2016
                     /locus_tag="IV142_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486244.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(2092..2427)
                     /locus_tag="IV142_002660"
     CDS             complement(2092..2427)
                     /locus_tag="IV142_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8486245.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            2554..2769
                     /locus_tag="IV142_002661"
     CDS             2554..2769
                     /locus_tag="IV142_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8486246.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            2806..3168
                     /locus_tag="IV142_002662"
     CDS             2806..3168
                     /locus_tag="IV142_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP8486247.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            3214..3540
                     /locus_tag="IV142_002663"
     CDS             3214..3540
                     /locus_tag="IV142_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486248.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT         1304 a          472 c          720 g         1103 t
ORIGIN      
        1 tataaggagg aaacacaaat ggaaacatca atgattaata ttccaattta tgtagatcaa
       61 gaaaaattat tagagaagcc agttacaaaa aaagaaaaaa ctgatgtaag agataggtgt
      121 ttagataact atagagaagt tacaagagat gagtttgtag agatactaaa ttcatcacaa
      181 tcatttattc caagcaagac aaagaataaa ggcaatgaca aggagagttt tgtggaaacc
      241 agagtaatta ttctggatgt cgataacact gttaaagatg agaaggggaa ggtagtagat
      301 ttgagtaaag atgatagtag atacttaagc atagaaaagg ttttatctat caatttagtt
      361 cataattctg cgtttgcgat acaaaaaagt attcggtact ctgaaaatct agaaaaatac
      421 aagattgtct ttctattaaa agaagcaatt actgactatg gtaaaatggt tgctgtttac
      481 gaatacttga aaaagaacat ccccgggtgt gatgacaatg taaatgcttc gaatagaatg
      541 ttttttggag gatataagtc agatgcagtg ataataatta atgaagacaa catgttagat
      601 attactgagc ttccgattga ttttaccatg acatgttcta aaaattgtca aagtgattca
      661 gggagagagt atgaactagt caatgaaacc gattttgtta agttgataaa aaatgacgac
      721 aaagaagaaa tgaaacagtg gtttaaagac tgcctgtttg attcctccga catgtctttt
      781 aatgagattt atgaaagatt gttaaccatt gatatgaata aattacttaa gtcaagtaaa
      841 aacttaagat gtctgtttca caacgatcat aatccctctg ctagtatttt taccagtaaa
      901 actgggcatt ccatttttta ttgtcattcc agtaattgtg atatcagtga aaattttatt
      961 ggagttgtga tgctaatttt aaagaaagac agtcgggtag aaacatttaa ttggctatta
     1021 gagaacctag acttgtatcc tacacatttt cgaaaattga aagaggaatc aaaagtactt
     1081 tttgacactc ttgaatcaat gaaagacaaa cttttcagga caataaggaa ttatctagag
     1141 gaaatgcgac gtgtctatga tgtcttgctt tctgatgtta atttttttga tgatagtaag
     1201 gaaagtgtga cttgtatttt gtcgggggaa caattagcta aaaaaatgag ttcttactat
     1261 ggtaaaactt atgacataga taaatgtaat aagttactat cacttatgac ttttatagga
     1321 ttgataaaaa aattagatga tgaagatgtt cctttgaaga tgctcgaata cctaaatagg
     1381 ttaaaggaag agaaagcgtt aggaaacttt aatcataaga gaagcaatgt ctatcgatta
     1441 gacgttttta atgcaacttc tgtgattcaa aaattgaatg aagatatcct tccacttctg
     1501 gaaaaaattc attttacata tcaatatttt tcttatgaat gggttaagat atgttttaac
     1561 gaaaaagagg ctaaaagagt atttcctcaa aattcccgat cagaactcag caaggaaaaa
     1621 caattgattt tagatgaagc acatgaagtt ttacagggct tatcgtggca agggacatat
     1681 ctaatgacag agaaagaact tattaacact gtttgtgaac gcctacgtaa tgttggggat
     1741 aacagcgtga aaaatgcttt aataaaaaac agaggctact ttgttaaaca aggattcact
     1801 cttgaacgag cttctaagga tattaagaaa ctatacaatg tgaaagcaag tagtagtttt
     1861 ttaatatatt tagcgaaagg ggcagggata gatgaaacaa taaaagaatt gatattatca
     1921 ggagctggga tagttcctct gtatcaattt acaagtgtaa caagagatga tggacggaga
     1981 gtaaggaaga gaaaagaccc aattgtatta aactagacag gttatccata gctcttaatc
     2041 ttatttcatc tcaatacacc caccgttaaa gttcggtggg ttatttattt ttcaatcttt
     2101 caatagctga ttgcgttcat tttcttgcct ttttaatagc ttcatatagt caattgttcg
     2161 aactgcatct tcagtgctaa gagaacgata gagagccaaa aactcttcct cataatctac
     2221 gacaacattg tctagaaaat ggcttacagg ggttcctagg gctctagaaa gagctgttaa
     2281 gctatcgagg ttaggctctg ttacagaatt ttcccatctt gctactgcct gttgtgtaga
     2341 gaagattttt tcagctaatt ctttttgtgt ccacccgaga gcttttcgct tttctctaat
     2401 gacctttgca tattgtttct gtttcattaa tacaccccca tctcagtata gtacaacaag
     2461 ttgttgtatt tgtaatggta tttgaagttt tttatatttt atgagttgaa atacatcata
     2521 ttgttgtata taataaaaca ggaggggaga atcatgaaaa aagtaagtaa tctcagccga
     2581 tacagaaaag aaaaaggact ttctcaaacg gagttagcaa aaaaaatgaa tgttacacag
     2641 caatgtgtaa gttcgtggca aacagggaga acgattccta aaccctatca aatgaagatg
     2701 ctttctgaga tattaagtgt accaatgaat gagctgtttt ctgatgtatt taataaagta
     2761 aacagttaac taataagcaa ttgaatgatt ttaggaggag aagacatgta ttgtagaaaa
     2821 tgtggcaaag aaataacaga tgaaagtaag tattgccagt tttgtggagc actacaagaa
     2881 acagatgaga caaccggaac agacacagta aagaaaagta ctgagcagac aattaatgaa
     2941 atgaaggaac aaatcgtaca tggacaagag acagggaagc tgtatttgat tattggttgg
     3001 atttctatgg tagtatcgtt agcatttatt cctgttgtat ttggtgctgt tgctgttatt
     3061 atgggctact tatatcgaga aaaagatgag aaaatgggta cgattttgat gatagcaggg
     3121 gtagctggag caatctttgg tgtactttta gggatgtcaa tgtactgata agagggataa
     3181 gaacaaatta ttttggagat aggagataaa aagttgaata aagtaaatat gaacaagaag
     3241 tttgtcatta taggtattgt tgtcttggct attataggta ttattttttc aaaaatgaca
     3301 ggtacatctg gattagagaa gtcatcaaaa ccattggtaa cacagatttt acaagaacag
     3361 tacaacttaa atcgatcatg tgatgatgtg acgattactc atgaaaatgg agataacaca
     3421 taccgggcta aagctatatt agacaatgga agtgctatca acataaatat tgagtactat
     3481 ccaaagaaag accgtatata tgtggagata ccatatgctg aggtgttaat gttaaattga
     3541 tttaaatgta ttatgcatac cgtccaaaca ggaactccca aaggtgtaaa ttcactagg
//
LOCUS       DACDEG010000152         3512 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.157, whole genome shotgun sequence.
ACCESSION   DACDEG010000152 DACDEG010000000
VERSION     DACDEG010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.157"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(1614..2132)
                     /locus_tag="IV142_002664"
     CDS             complement(1614..2132)
                     /locus_tag="IV142_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP8486249.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            3250..>3512
                     /locus_tag="IV142_002665"
     CDS             3250..>3512
                     /locus_tag="IV142_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8486250.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTSGSFKEKDNLTPEQKELLELRKRNQQLEMENDILKQAA"
BASE COUNT         1202 a          615 c          516 g         1179 t
ORIGIN      
        1 aatcttaact gctgagattg atgtagcaat atctcaatca tcaacaaagt acgatcctgc
       61 ctttcaaaag cttattgata aatgtttatt cattataaaa ataaaagaaa aatttatgtc
      121 agcttttttc atcaaagtgg taatgataat tcgtctatta attgtatttt ttaggaaata
      181 tggtataatt gttttaacga aaaggtaatt catgatacac caaaaaccac tagctggcgg
      241 gctagtggtt ttcttttacg ttctattcag attaacgggt aggcttactg tcttttttgg
      301 caataatttt gcaatcaagc catttacaga tgtaatgcgc ggatacttct gctaagattg
      361 caaaaaacat atcgaaaagg taattcataa tacaacctcc tccccttgcc ataataggcc
      421 ggggtagcag acagcataaa tattatacca taactgaaaa tttcatttta gtacatttca
      481 atatattgat gaaccagcac aataaaacat gccatgacta acagaaaaaa acttattaaa
      541 ccagatataa cttgtaaaca tccaactata tagaaaacgt aaattttcta tatagttgga
      601 ttaagataac gactatttat tttatcatat ttttactata ccctatactt ttacttggta
      661 tatttatgaa taatcgcaaa tttaccccta tactactagt ttgtaagtta gattaataaa
      721 aatactaaag ttatttgttc ttttattaaa tacacctaca acttataaat tgatggtatt
      781 tagataacac gaaagaataa aaataacagc tgatagtaaa gtttgttgac gattgctact
      841 caatcgaaag tgagttagtg cgtatgaact tgcttctgaa aacttatgaa atgaaagacc
      901 ttttacatct tgcagaaatc gttactggga ttaaactaag caataaaaac taatgttccc
      961 ctcattcttt gaacttctac tatttgattt atttattctg ataaatgacg atttcacctt
     1021 caggatcttc aaatcccgta tagaatttat ttaagatctc ttcagtatca gtaataggat
     1081 aggcgatagg ccacataccc tttgatggtt agaataaact gctcaaaatc tgtatcgtat
     1141 gttggcttta gcatattaac aaaggctatc tccaaattat aaaccataag cgttgaatcg
     1201 ttcttaacaa tttcaattct tcctaaccca aagtcagaag atataattac cggacttgcc
     1261 ttacccttca tgtgggttgt tgagattcca tatttaaagg gaattgtcgg tactatcggc
     1321 ggtctgaaaa acatcccaaa aataaaaaaa tctttcccct gctagtctcc taagtccctt
     1381 tgtagcggat atggcaccat aatctcattt tatcgaagtg agtcatggtt tatctgttgg
     1441 ccaaccaaaa ttgatacaat ctaacgatgc accctctacg gcattctagg ggagtgcact
     1501 tgcattcacc caataaaaac cggcagttag caaagaactt atgtagtcgt atgttcttac
     1561 tgttaacata caagaggtct cctttccaca attgcttagc agtaaacggg aatttacaaa
     1621 gctaatctct tccattttct tatctttgtt ctaaaagtcc caaatggagc aactgtattt
     1681 acatgaataa atttatatac ttcccataca gccgttttgg ttgcctcgtc tgcccatttt
     1741 cttttgtgtg cagtgaatag atcttcatca ctcattgaat caatcatcat gttaatatca
     1801 tcaatattac gattgagaat ttcctctaat tctttcaatg gtagatgtgc ataggtattt
     1861 gtaaaccact tatataactc tcctaactga ttccatttaa attgatcaga aggcgtttta
     1921 acagataatc catttctttc atcattttcc cattttagaa ttaaattcgt ccaaccaact
     1981 tgatatgcta aattttcagc aggtgttcta tccacttcct caactctttt atccttcaat
     2041 tcttcaggaa tagtttcaaa ttctaaaata tatttagtat aagtcctatt tatttctttt
     2101 tttaattctt ctttattttt atatgttctc attatattac ctccataaat tcctaattgt
     2161 ctcctttaat tcatctataa tttcatccac gatattttaa tccgttctac aatgcatatc
     2221 tgatatgcat tgactgtcta actcatatgt gaatgattta taaaatatcc caacgtctca
     2281 tctaaagaaa tgttgatcgt cagcaaggag ctaaaaaata cgaatatatt tcttttgtag
     2341 taaaatcgga ctggtttgca tatcattttc ttcgtaaata tcaaaatcat gttaactact
     2401 atcaattact aataaaaact accactttaa gcgttaagaa ctgttaagtt tagttaagtg
     2461 aggatagttc acaaaatcca ttgttaaact tcacgaaagt ggtaaaactc aaaatgcttt
     2521 atataggatt acagcatcgc tctttcttcg attgcgcgtt gggtaaaaaa tattatgaag
     2581 taaaaattga cgatgacacg ttccttacgg ctcatcagat gaaacagcta caaaaacgaa
     2641 atgatcaact cgaagaaaat ttaattctaa aaaagcgatt gccatattca cgcctcgctc
     2701 gacgaactat taaaggtcaa gatttacctg aagatcatcc tttttaggaa attgaagctt
     2761 tttatgttgc acaatgcatt tataatacta caatgatgtt ttcaccagat attcttctag
     2821 ggtgcggtgt aatgaaacag atacacttgc aaaagaaaat tcaccgcgaa tttactaaaa
     2881 ttatgaataa ttatgttaat cttccgaaat ttttttccaa aattaaggga tcaagctggt
     2941 gttatagatt gtttaaggtt ggcaaaaaaa taacttcttt ttaaacaatt atagaaaacc
     3001 gatactctgt taagttggtt gacacggtgt cgatttttat gactttctta ttctgaaact
     3061 tcactaacgg acagtaccta aaaagtctat agtatggacg tttacgagta ctattttatc
     3121 aaaaatgctg ctcatttcaa cttatatgtt ttagtgaact acccccactt agcttggata
     3181 ggctacaatt aactagacag aaaaattaag gtgtgtagac tagaagaaaa cataccagga
     3241 ggaattttta tgtctaagag aacacgaaga actttttcac aagaattcaa gcaacaaatc
     3301 gtcaatcttt acttagctgg aaagccacgt gtagaaatca ttcgagaata tgaactaacg
     3361 gcttcagcat ttgacaaatg ggtaaagcaa tctaaaacga gtggttcatt caaagaaaaa
     3421 gataatctta cgcctgaaca aaaagaattg ttagaactac gtaaaagaaa ccagcaatta
     3481 gaaatggaaa atgatatttt aaagcaagca gc
//
LOCUS       DACDEG010000153         3497 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.158, whole genome shotgun sequence.
ACCESSION   DACDEG010000153 DACDEG010000000
VERSION     DACDEG010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.158"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(142..1344)
                     /locus_tag="IV142_002666"
     CDS             complement(142..1344)
                     /locus_tag="IV142_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HAP8486251.1"
                     /translation="MCTKLLQNTAHKNPYKTVDEATQLDLVIVVNQLLTGYDSKWVNT
                     LYLDKSLEYQNIIQAFSRTNRLNGPEKPFGTIRYYRKAHTMEQNIEEAIELYSGKQAK
                     GLFVEKLYNNLEKLNDIFEEIKSVFNISGIENFSHLPEVPGEIKQFVNLYNQLIHCYE
                     SAMIQGFTWAKSTYEIQDYEPITVVITEYEFNILAQRYSEIEREPRDGNDSIAYDIDY
                     QLPEGSRSRINYEYLNTNFKKYMNIKEERDSNLIQSLLNDLHRTFAQLPVELQPYAEM
                     ILNDVNNGELVIKEGWEFTDYLNEYQLSEEDDFIQDLVDSTNINEALLREMLDLRLTE
                     VNINEYSRFDKLKSSVNVGHFSGYIEKNLGKMVIPIKVNMLIDRLLRAFLLEDVFDVT
                     EYIKNYFN"
     gene            1365..2138
                     /locus_tag="IV142_002667"
                     /pseudo
     CDS             1365..2138
                     /locus_tag="IV142_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            2317..>3497
                     /locus_tag="IV142_002668"
     CDS             2317..>3497
                     /locus_tag="IV142_002668"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8486252.1"
                     /translation="MSMNHFIKQLFTIKDSNIQIQSFEETFYKSRKSIVIEGTLIRTY
                     RRCPCCKDSTKQIVKNGKKISMILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLV
                     TPFCFISKQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPR
                     CLLFDEFRSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHY
                     IVTDMNAPYFSLVKECFPNAQVIVDRFHIVQHLNRFFDAVRKRVMKTLDQKDPIQAKQ
                     YRQLKSLFKLLLKSEDRLDYNTLTKRRNFNWRLLTETEVVDRLLKISPELKTAYRYYQ
                     DLLTAYQEKDPDTFFQLLRAMPGEVPLELHHIKKAFFKYEKGIRLALLKKYSNARLEN
                     L"
BASE COUNT         1082 a          686 c          588 g         1141 t
ORIGIN      
        1 ataatccttt ggattatata cggatacaga tgtagacata aaacaagctc aattttcaaa
       61 aatggcatta ctcacctttt tggtgaatta ttaaactaaa aaagatgaat ttttttatgt
      121 actataagat gtaggtgtac tctagttaaa gtaattcttt atgtactctg taacatcgaa
      181 tacatcttct aataaaaatg cacgcaataa tctgtcaatt agcatattta ccttgattgg
      241 tattaccatt ttacctaaat ttttctcgat atatccactg aaatgaccta cgttaacact
      301 tgattttaat ttatcaaatc gactatattc attaatgttt acctcggtta agcgtaaatc
      361 caacatctca cgtaataatg cctcattaat attggttgaa tccactaaat cttggataaa
      421 atcgtcctct tcactaagct gatattcatt gaggtaatca gtaaattccc atccttcttt
      481 gataactaat tccccattat ttacatcatt tagaatcatc tcagcatatg gctgtaactc
      541 cactggtaat tgcgcaaatg tgcgatgtaa gtcgtttaac aatgattgaa ttaaattcga
      601 atcacgttct tcttttatat tcatgtattt tttgaagttt gtgtttaaat actcataatt
      661 aatacgacta cgactacctt caggtaattg gtaatcaatg tcatacgcta tactatcatt
      721 tccgtctcta ggttctctct ctatctcgct gtaacgttgt gccaagatat taaattcata
      781 ttctgtgatt actactgtta taggttcata atcctggatt tcataagtcg attttgccca
      841 tgtgaatccc tgtatcatcg ctgattcata acaatggatt agttgattat aaagatttac
      901 aaattgctta atttcccctg gaacctccgg taaatgactg aagttttcta tgccagaaat
      961 attaaatacg gacttaattt cttcgaatat atcgttcaac ttttctaagt tattatataa
     1021 tttttcaaca aataaccctt ttgcttgttt gcctgagtat aactcaatag cctcttcaat
     1081 gttctgttcc atcgtatgtg ctttacgata ataacgaatg gttccgaacg gtttttcagg
     1141 accattaaga cgatttgtgc gtgaaaaagc ctgaattatg ttctgatatt caagtgactt
     1201 atctaagtat agggtgttga cccattttga atcgtaaccc gttaagagct gattaacaac
     1261 tataacaaga tctaattggg ttgcttcatc tactgttttg taaggattct tatgcgctgt
     1321 gttttgcaat agttttgtac acaagattgc aaagaaaagt acacaatgtt gccacttctg
     1381 gttccatgag ttttatttgt gttgatggga caaccaatca gctatttacg acccttgaag
     1441 atcgcagact ttacaaacta actcaatact tcctgcgctt tcctagaaag gcccgtttaa
     1501 aggtcaagta cctagtgatg gacatgaatg ccagctattg tcagttgctt aaaacggtct
     1561 ttccaaatgc ggagatcgta actgaccgct ttcatattgt ccaacacatc aatcgcagct
     1621 tcaatcaatt acgtgtccag atcatgaatc gttttcgaac ctcacactct gaaaatcaga
     1681 agaagtaccg tcggctcaag cgatattgga aactcttact caaagattcc acgacatggg
     1741 aggcactcaa acggcattat catcgactgt ttaaacgacc aattagtcaa acagaaatcg
     1801 ttaatgagct gttaagctac aacgaagaat tgcgaactgc gtatcatttt tgtcaattat
     1861 tacgctatta tttcgttaaa cgagatacgg taggctttca agaaaccctt aaaacaattt
     1921 catctacgtt acctcagtcg tttaaaaaga aattcacgat cttccatcgc taccaatcgg
     1981 gtatttcaaa tgcctttaag gtgtcccact caaacggcgt cactgagggg ctgtataaca
     2041 agatcaagct gattaaacga attgcgtttg gttatcgcaa cttttataac tttcgggcac
     2101 ggatctattt acaacaaggt ctgatctttg aaaactaaaa aaatatgcgg cgcacagaac
     2161 gcgccgcata tcggactaag ttttattggc tctataattt ctaggactga tggaaatttt
     2221 ctatctgtcc tagttttttt gtaaaaaaac aaaaagacat acaaaatttc cggtagaata
     2281 gagtctccta caacatcatt ctgggggaat tcttgtatgt ccatgaatca ttttatcaaa
     2341 caactcttta ctattaaaga ctcaaatatc caaattcaat cctttgaaga aactttttat
     2401 aaaagcagaa aaagtatcgt tattgaggga actctgatcc gtacctacag acgttgtccg
     2461 tgttgtaaag attctacaaa gcaaatcgtt aaaaatggca agaagatctc tatgattctc
     2521 ctgaatcgta gcggtaataa acggacctat cttcggttga agaaacagcg atacctctgc
     2581 cgagcatgta aaaaatattt taccgctcgg acgtatctcg ttaccccatt ctgctttatt
     2641 tctaagcaga ttcattacaa gatcttagaa gaattgacag agcgtcaatc tataaaagca
     2701 attggtaaac attgcgatgt ttctgtgaca actgttcagc ggacattgtc cgccttgtca
     2761 tcagatatcc gtcagaatct tgagtattta cctcggtgtc tattattcga tgagtttcgt
     2821 tctctttcta catggcacgg taaattttct ttttcttgta tggacggtga gaccgggaaa
     2881 ctgtttgaga tcttacctag ccgccgtaaa aaagatctcg ttgctttttt catgcgtttt
     2941 tctctcaaag cgaggttggg tgtacattac atcgtaacgg atatgaacgc tccttatttc
     3001 tcactagtta aagagtgttt tccaaatgca caagtgatcg tcgatcggtt tcacattgtt
     3061 caacatctga accgcttctt cgatgcggtt cgaaaacgtg tgatgaaaac acttgatcaa
     3121 aaagacccaa tacaagctaa acagtatcgc caattgaaat ccttattcaa attactttta
     3181 aaatcagagg atcgtttaga ttacaataca ctaacgaaaa gacgcaattt caattggaga
     3241 ctattgactg aaacagaagt agttgaccgc cttctaaaga tttctcctga acttaaaaca
     3301 gcttatcgtt actaccaaga cctgcttact gcgtatcagg aaaaagatcc agatactttt
     3361 ttccagctgc ttcgagcaat gcctggagaa gttccattgg aactgcacca tatcaaaaaa
     3421 gcctttttca aatacgagaa agggattcgg ctggcattac tgaagaagta ttcgaatgca
     3481 cgactggaaa atctcca
//
LOCUS       DACDEG010000154         3419 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.159, whole genome shotgun sequence.
ACCESSION   DACDEG010000154 DACDEG010000000
VERSION     DACDEG010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.159"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            164..715
                     /locus_tag="IV142_002669"
     CDS             164..715
                     /locus_tag="IV142_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HAP8486253.1"
                     /translation="MKRLEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDN
                     LKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKV
                     MTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYA
                     VPYVFFAIILWLIALFFRVFNDL"
     gene            1391..1657
                     /locus_tag="IV142_002670"
     CDS             1391..1657
                     /locus_tag="IV142_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486254.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQDPLC"
     gene            1715..2227
                     /locus_tag="IV142_002671"
     CDS             1715..2227
                     /locus_tag="IV142_002671"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8486255.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLC
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            2224..3261
                     /locus_tag="IV142_002672"
     CDS             2224..3261
                     /locus_tag="IV142_002672"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8486256.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1253 a          577 c          678 g          911 t
ORIGIN      
        1 aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa tcaaggtatt attgggaatc
       61 ccagcttaaa tcatagatac cgtaagggat tttattcttt atttaaaact ttgcaacaga
      121 accgaagagc gagcaagaca ggaacgagag aacaaacata ctcatgaaaa gactagaggc
      181 ttcgacctcg aactttaata ataagttaga tgtttttgaa gaaaagacta agcatgcaag
      241 attaaacttt gaaacaactg caaagcacta tattaaacga cttgatgaag ataatttgaa
      301 attagatttt caacaagcta ttcaagatga attaagcgat actaaagacg aaatacgtga
      361 agtgacgaaa caagcgagag aagaaacaaa agagtataaa gaaatcttag aaagtaaaat
      421 aaaagaccat aacaaagtgg ttgataaatc gaatacagcg ctaaaagtga tgacaaaagg
      481 cgttacaaat atattttttg tgctaattat ttttgtactt gtaatgcttg tgacaggtcc
      541 gataggtcat ttctttggta ttgaacattt atatagcttt attaatggtt ttattgatga
      601 ccacgaaagt gcatggcgat atttgatgtt gatactttat gcagtaccct atgtattctt
      661 cgctatcatt ctgtggctca tagcattgtt ttttagagta tttaatgacc tttaggtaga
      721 tttatatata aaaagggcta aatacgcaaa ataccccctt aaaatgcaaa ataaatgcaa
      781 ataagggggt aactccttat gaaaaaacaa ctaatagtat tataacacgt ttttatgccg
      841 agaaaactta ttggttggaa tgggctatgt gttagctaac ttgttagcga gttggttgga
      901 cttgaattgg gattaatccc aagaaagtac caactcaaca acacataaag ccctgtaggt
      961 tccgaccaat aaggaaattg gaataaagca acgaaaggag ttgagtaaaa tgagtgagaa
     1021 aacgaagaaa atcattcgag tatcattagc tttttcacaa gtagtagtgc aatcattgat
     1081 agtttattat acttaccagc aatataaatt atctaaagag aattaagttt tgattatgta
     1141 ataagttttg gtactaaaaa agacttgatc taattagacc aagtcttttg ttagtgttat
     1201 attaataaca aaataaaaat aagaagtcgc tcactccccg accaaagttt gtgagcaaaa
     1261 taaacagtcg atgtttgttt cttaattgta tatcttgata ttaaacgatt taagattatt
     1321 cttcaagata tatattcgca aatgtaattg gaaaagcgac ttcttaaaca aaattaagga
     1381 gtcgcttttt atggcaaaaa taacgaaaaa tgagcaagaa aatttggtgg aaaattacac
     1441 aaaaaaaccg gcgtactcta atagccggtt agacgcacat ttcgtgtgca cttctaatcc
     1501 gaaattgagc tttgacgcta tgacgatagt cggaaacctt aataaaaaca gcgctaaaaa
     1561 gctatccgat tttatgagtt tagatccaca aattagactt tgggatatac ttcaaacaaa
     1621 atttaaagct aaagcactac aagaccccct atgttaggct agttgtcgta taggataata
     1681 agttataata aagactatac ggaggatgat taccatgaca aataaaaaca atgaacgtta
     1741 ctctaccgaa ctgaaagcga cagccatcaa gaaaatgatg ccacccgaaa atagggggat
     1801 tcgccaatta agtcaaactt tgggcattcc agaagcgact ctttgttctt ggcgtaaaaa
     1861 agctagaatt caagggaata ctacacccgg aacgaaaaaa aatgtcgaaa aatggtcaag
     1921 tgaagataaa tttttaattg tgatggaatc attctcaatg aaccaagtag aacttgcgga
     1981 atattgccga aagaagggct tatttaaaga acaaattgag gcttggaaga aaacgtgctt
     2041 atctgcgaat gaacaggaag aaaatcgaac gagagaactt gcaactgaac taaaagaaga
     2101 gaaaaaacaa gcgcgtcaat tagagaaaga attacgtcat aaggaaaaag cattagcgga
     2161 agccgctgct ctcttattac taagaaaaaa agcacacgcg atttgggggg accaagagga
     2221 agaatgattt catcttcaga tcgcaaacaa gctgttgaac ttattcaaga agccaatcgc
     2281 aatggggccc gtctcaccta tgcctgtaat gagctgaata ttagcgttcg aacctacgaa
     2341 cgatggacgc gtgaaggtac gatagcgcat gatcaacgcc ctttggcgaa acgtcctgta
     2401 cccaaaaata aactgacgga tcaagaacgc gaaaaaataa tagaaaccgt ttcaaaaaaa
     2461 gaatttatga atttgccacc tagccaaatt gtaccaaaac ttgcggattg ctcgatttat
     2521 attgcatcag agtctagctt ctatcgtgta ttgcgagaaa aaaatatgca acatcaccgt
     2581 ggccgaagtc aagtttcaca aaaacgcata ccgccaagtc accttgcgac gaagccaaat
     2641 gaagtttgga cttgggatat tacttggcta aaaggtccaa taaaaggact cttttaccgg
     2701 ttatatctca tcattgattt atttagccgt aaaatggtag gctgggagat ttgggaatca
     2761 gaagaagctc gtcatgccga aaaacttatt aaacagacgg tgcttaagga acgtatccaa
     2821 ggacagccgt tggttttaca ctccgataat ggcagcccta tgaaagccgc gacttttcaa
     2881 gtcttactcg aaaaattagg aattcaaagt tcttattcaa gaccacgagt gagtaatgac
     2941 aatccttatt cggaagcggc tttccgaacg ttaaaatatc gaccagaata tcccaataac
     3001 ggctttaaaa caatagatga tgctcgaaaa tggacgaaag agtttgtcga ttggtataac
     3061 gattctcatt tgcatagtgg gattcaattt gtgtctcctg cagcgtgtca tgaaggacga
     3121 catctcgcta ttttagagaa acgaaagcag gtttacgagg acgctaaaaa gcgccatcct
     3181 gaacgctggt caaaaggtgt tcgaaattgg actccacatc aatcggtggc gttgaatcct
     3241 ttaaaagaaa cagaaagtta acaggagcat aatctttcaa gattatcaaa taaccctcgt
     3301 cacccaatag gggaaaaagt tattcttccc ctattggata acgaggccaa ccgaccatag
     3361 ggaggttctg ctttttgata ggtggaaaat aaaaatacga caactatatt gacaaacac
//
LOCUS       DACDEG010000155         3381 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.160, whole genome shotgun sequence.
ACCESSION   DACDEG010000155 DACDEG010000000
VERSION     DACDEG010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.160"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..2303)
                     /locus_tag="IV142_002673"
     CDS             complement(<1..2303)
                     /locus_tag="IV142_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486257.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVET"
     gene            complement(2975..3243)
                     /locus_tag="IV142_002674"
                     /pseudo
     CDS             complement(2975..3243)
                     /locus_tag="IV142_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          918 a          761 c          548 g         1154 t
ORIGIN      
        1 gtttctacca gctgatatgc tcctggtgct aaaccgtcaa ttgccagttt tccatcttct
       61 cctgtagtta aaccggtttg tagcgtttct ccttctcggt tctgtaactc aaaaacagct
      121 ccttgaagga cttcaccact gccttcagca tgtttaatta gttcaacacc acccgtatca
      181 aggatgtttt caatggttaa cgtcacttct tctatggagt attcatttat aaaaacatca
      241 tgtaccgtcg catctaaaag atacccttcc ggtgcttctg tttctcggac ttgatagcta
      301 cctacaggaa gcggttcact taaaaattct ccgttttctg ccgttatacc ttcttgtatc
      361 acttgtgaat cgcttgataa aatttcaaat tttgctcctg ccagcctttg gccattctca
      421 tcagttttaa ttactttaat acgcccatcg attttccttg ttcttacagt actcgtattc
      481 aatcgattgt cactattcac aagcgttgca aagtttaaaa tttgatcgcc gaccgcatac
      541 gcctctacat tgaccttaaa agagagggta aaacttgtcg gaccaccaat cgtattattc
      601 gcactgaaac gaactattcc attattgact tcggcatcaa tgaaagaggg aatatctgat
      661 agaaatgaca caaattccat gccttcttcc aaaacatctt caataacaag cgtatttctt
      721 gttccagaaa acatcgagac atgcagagta tacgttagtt cattttcatt gagattgata
      781 acatttttgt ccacactttt tcttaaagag attgccggaa gattactttc agggattctc
      841 aagcggtagt ctcggctcat tacctgatta ttgtaggtca tactcgctcg attccgagaa
      901 aacccatcct cttgtcttcc ttcattgaac aagtcgacat cggtcacttc aagagcatat
      961 tcaataaaat acgattgacc ctctgctaga ttaccaaagt ccacaacaag tgtattcgtt
     1021 tccttattaa aagatacttt gtccacaaat tcctcggaaa cataatgagt tctaacgttt
     1081 gcttcacctg gattcgttac ttctgcgatc cgaaccccac ctgaaatagg ttctctattt
     1141 ccagtggaac tccatgtagt cggattcggt gccaaagttg ttccttgcgg tagtacatcg
     1201 gtaaccacta caccgtcttt tcgtgatcct tcataattta cctgcaaaat aaatttatta
     1261 ctgttggtca cgtcttgccc gtcaatcagc agattcccaa gatttccttg gctgtcgatt
     1321 tctctagagc catagtataa tttcgaaaaa atgggtcttg atgaaggaat aggcgtaatt
     1381 gaaaattccg tgtttgctgc tcgctcagca taagtaagac tcacttgaaa gtcattattt
     1441 ggctcagtac cgcctgattg aaagaaagca acttcaaacg tgatactgaa tgtatacgac
     1501 tgagcggctt catttaatcc ttcataatca aaggtaaatg gaaaaacaac gtctgtgcct
     1561 tcttggtaaa atgccagtgg ctttaaaggt gaatcatcag cgacctccac tctaatgaac
     1621 tcaattaagc caatcgatgc tctaggaact cgtacgacca tctccccata ttcttcaaga
     1681 atatcgccag cgcctccttg aatgtatccc tttaacgttt gtgtatgcct gctcggcaaa
     1741 gtggggcgct cagcagagat gccgatgtca taattcaaga gctcctcttg tctttcaatc
     1801 tgagattcct gcgttataga tggctcagat gatctggctt cattaaaaga ttcacttaag
     1861 ctttcttctg gtatattaaa agattcactt gtgctttctt ctggtacatt aaaagactca
     1921 cttgtgcttt cttctggtac actgatttct attgagaccg tatttgattg aatatcaccg
     1981 cttcttgcgg tcaattcgta tttacctgaa tgctgggaag aataagtaat cagacgttcg
     2041 taatcattga gttgcttcac ttccccttca atcaaatcct cactcttatc aatgaccaat
     2101 gcatttagca ggtctggtgt tcctctgact cgcaactgga actgctcacc caaagcagcc
     2161 tcctcttgat caacttcgat cgtcagcgaa cgctctggct gttctgctaa agactcagct
     2221 agaaccgtta tgttcccaaa ttgattgatc acaataagca taactagaaa catacttaac
     2281 agtttatgaa atttgttcct cactattact cccccttata attttgacta aacaacaagg
     2341 tacccgataa ttttctaaaa agattcccca actcaaaatg gctgcgttac ctccctcgta
     2401 ttttcggttc aactcaagcg ttgcaatgta agctgtaaac aaagctaatg attgccgatt
     2461 gtcttctgtt gattgattcg atagctttgt atgactttta gttgtccgtg tggcttaacc
     2521 ataattatga tttcacgatt cctcctgcca gtgccttgca caagctggag cgtaacccta
     2581 atagttattt tgttaatgta cttatatact tttatacttt tccattagaa acaatcacta
     2641 aatcgttgat accaaggtca gactctagaa tttcatcagt gagtccctgt attggttaac
     2701 cttcatcaat cccaaaaaaa tatatcaatg cttagcatca aaatgaatat atagtttaga
     2761 atacttggga ttcatcgata actgatcgct tatttggcca tattatttgg cacttggttc
     2821 aactaaacgc atttaggatg ttccttcatt ctcgtcatgg atgttctcaa accgtcatta
     2881 ctaataaact gtccaccgaa tcgatggttc atttcttgca ttttccacgt ccaattactc
     2941 tttttgctct ttatttgggc gtagtttcac tttcttaact tgtatcgtct tccatcaact
     3001 ccttaatcat cttcttagct ttattggctc ggtttccttg taaaaggtat ctacaaatag
     3061 tgacaatctg tattaaatct tcatcatttt ctgtaatgtc aataatttct atattctgta
     3121 ccatatattg caaagggttt cactaagttc ataaccgaag cgaattaaac aacttttgta
     3181 tagaataact accttttcta tttttccatt ggtaattcta tctattaatt ggtttaatcc
     3241 ttttgttgta gttgattcca ctaccaagat gattctctcg aattttaaag cttgaagatg
     3301 tattgctatt tttgctaact ggcgtatttg caactatttt tcccttctat tcttccttca
     3361 ttgattcccc tctaaaatta c
//
LOCUS       DACDEG010000156         3358 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.161, whole genome shotgun sequence.
ACCESSION   DACDEG010000156 DACDEG010000000
VERSION     DACDEG010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.161"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            95..1174
                     /locus_tag="IV142_002675"
     CDS             95..1174
                     /locus_tag="IV142_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8486258.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            complement(1475..1717)
                     /locus_tag="IV142_002676"
     CDS             complement(1475..1717)
                     /locus_tag="IV142_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8486259.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(1749..2306)
                     /locus_tag="IV142_002677"
     CDS             complement(1749..2306)
                     /locus_tag="IV142_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8486260.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(2319..2507)
                     /locus_tag="IV142_002678"
     CDS             complement(2319..2507)
                     /locus_tag="IV142_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP8486261.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(2520..3086)
                     /gene="amaP"
                     /locus_tag="IV142_002679"
     CDS             complement(2520..3086)
                     /gene="amaP"
                     /locus_tag="IV142_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP8486262.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT          927 a          767 c          679 g          985 t
ORIGIN      
        1 aattaactta gccatttgga aataaaaaaa cgccacgtga attccatgtt atattgaagt
       61 taccacaact ctcaatagac aaggatgaat tcacatgacg caaaaccatg ataacacagt
      121 ttcacgcaag ggaaaacacc taacctactc agaacgttgc caaatcgcca tcctgaaaaa
      181 ggaaaactat tccaaccgtc aggttgcgga tgtcctgggc tgcgtgccgc agaccataaa
      241 caacgaaatc aaacgcggga ccgttactca gcttaaccgc cagaatcaga acgggaaggt
      301 ctacgactat tatcatgacg tctacgacgc cgatgccggg caggcggcct acgagaagaa
      361 ccgcttgaac tgcggcagac ggcccttatg ggccgacacg gacgccttca ttgaatgggc
      421 ggacgacatg atgctgttgg aaaaatggtc cccggacgtg gtggtgggct ttgccagaaa
      481 acacgaattg ttcgacccgg ccattatccc tgggaccacc accctctatc agtggatcga
      541 ccgtggcatc atgaaaacga agaatatgga cctgctagaa aaactgtccc gcaaaccgaa
      601 atcgtcccgt catggcaacc gcccgaataa acgggtgcta gggaaatcca ttgaagagcg
      661 cccggaggcg gttggtaccc ggaaaacctt cggccattgg gaagtcgaca cggtcgtagg
      721 aaacaagacc aagtcggatg ccgtgctgct gacgttggtg gaacggcaga cacgcttcga
      781 ggtcattctg aagctggccg ggaaggatgc cgaatcggtc aaccaagcgg tccgacagct
      841 gagagaacgg gctggtgcgt acttcggcca gctgttcaag acggttacct cggacaacgg
      901 ctcggagttc gcgggactgc acgatgcctt gcaggatgtg gcaaacatct acttcagcca
      961 cccctatgct tcctgggagc gcgggacgag cgaaaaccaa cacaagctga ttcgccgttt
     1021 catccccaag ggcagcccaa tcggcaacgt gagcgaaaac caaatcttgc ggattcagcg
     1081 ttggatgaac gattacccgc gaaagatttt gggctatgca acgccgcatg acacgttcgt
     1141 ccaggcattt aggcaggaac ggttgacggc ttgacacgtg ggcccttgac aacgagcctc
     1201 cctcggcatg gcttgtgtct ggaaagctgg ccggggccag ccagtcggga gactaccatg
     1261 cctcagctca ttgtcaaggg cccactcgat tagaaaaatt agacggttgg tggtaacgag
     1321 caaccgggtc accatgggtg gctaacttaa acttgaaatt tacgtaagga ataaagaata
     1381 cttcatataa aatatataaa aaagaaccgc agcgagtgtc gctacggttc tcaaatttgt
     1441 tcttcatttc aacataagtt tttcttcaat ttgcttataa tttacgtgca aagaatgaaa
     1501 cgactaatac taaaatgatt gcaccgatca atgatggaat gatagccatt cctgcaattg
     1561 ttggtcccca agttcctagt aaagcttgtc caattgctga acctactaaa ccagcaatga
     1621 tgtttgcgat gatccccatt gatgatcctt tattcgtgat tgctcctgca attgctccaa
     1681 tgataccgcc gacgattaat gcccaaataa atcccatttt aaattcctcc taactgactt
     1741 cttacttctt attgtacacg tggttcgtta ttttctttga ctttttgtgc acctttgcct
     1801 acagcagatt ttgctttatc tgtttggcgt gaagcaaatt gtcctgtgga ttttgctgct
     1861 tctgttactt tgtcttgtac tgtttcttga tctttttggt attcttcttg tgttttgatg
     1921 tctgcaacat tcacattcac ttcgataaca tctaaatgtg tcatattgtt tacttcagta
     1981 ctgatcaatt ctttgatttg gttgtaaata tcttcgatgt ctttaccgta ttctacgatc
     2041 acatctagat ccacagctac ttgtttctta cctacttctg tattgatacc tgctgttacg
     2101 ttatctgtgt tgactaattt ttcagccata tttgagaaaa atccgccatc taatgtcaat
     2161 agaccatcta catctttcaa tgcataaccg atgatttttt ggatgacttt gtcttcataa
     2221 gtcaattcac cattcatcat gcggttttct cctttttcat ttgccaaaac tgatccattt
     2281 gttgtattca tgtttttgtt ttccatcttt gtttcctcct atgatttaaa aaattgatca
     2341 actattcctg actgtttcaa ataaaaaccg attcctgcgc caattgctgt catgattatg
     2401 atcaacaatg tcttgaaaaa tccaatcgtt aaccataaaa cagctagcag tagtccaaga
     2461 cccccaaaga taatagggag cttgtatgct gaaaatagtt ctttcattat ttggccctcc
     2521 tattccactc gagagtgctt gtagtctctt gtatcttctt gttcttcata accgctgtat
     2581 gtgactgcca ctttgaccgt ttcttttgtt ccgagtactt tagttacttc ttcttcgata
     2641 tcttgcatca atacacctgt tttccctact aatgaagaag ttcgtttcaa gtctccgttg
     2701 acatggatat ggattcgatt tttcgttgaa cgtatccgta ctttaggtga atgaatgaac
     2761 tcttcttcgt gtaaatggga acgtaccaac ccttcgatcg cttttttgtc gattgttagt
     2821 ttgccatctt cccgtttcat aacgaaagtt cctcttgact ttggatagaa aagaacgact
     2881 agcaagatga taatagcaag aacggcaaat actacagctg cccagaaaat atattgttgc
     2941 atgtagtaat tcgttaaagt aaagaagcga aatcgttcca attgttcagg cataatatac
     3001 ggcgcattga tcaacgcaat cgctagaaat actgaaagta gcagtaaact catgataaca
     3061 ccgattattt ttaacccctt attcattttc cacctcctat aaacttattt ttaccctgta
     3121 aataactcta ccgacaatca cagtatagat agcgaacagt tagcatagca tcagtaaata
     3181 aagcgctacc aaacattgct ggatcataac ggcgttttat tgccagtctg atactgaaat
     3241 aacaattgct tacttattct ctatttactt ttgattgctg gctatactta aaggatatta
     3301 ttcaagatta aaaattgcaa agattatgct cagttattat tacaagattc tatttatt
//
LOCUS       DACDEG010000157         3329 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.162, whole genome shotgun sequence.
ACCESSION   DACDEG010000157 DACDEG010000000
VERSION     DACDEG010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.162"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            38..718
                     /locus_tag="IV142_002680"
                     /pseudo
     CDS             38..718
                     /locus_tag="IV142_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(775..1206)
                     /locus_tag="IV142_002681"
                     /pseudo
     CDS             complement(775..1206)
                     /locus_tag="IV142_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1765..2082)
                     /locus_tag="IV142_002682"
     CDS             complement(1765..2082)
                     /locus_tag="IV142_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8486263.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(2083..2337)
                     /locus_tag="IV142_002683"
     CDS             complement(2083..2337)
                     /locus_tag="IV142_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8486264.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            complement(2597..2815)
                     /locus_tag="IV142_002684"
                     /pseudo
     CDS             complement(2597..2815)
                     /locus_tag="IV142_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT         1097 a          594 c          535 g         1103 t
ORIGIN      
        1 gtgtaaaatt gagaaaaaag acagagagga cagagtaatg aatcatttta aaggcaaaca
       61 attcaaaaaa gacgtcatta ttgtcgctgt tggttactac ctgcgttaca atctaagcta
      121 tcgtgaagtt caggaattgt tatatgatcg tggaataaat gtttgtcata ctacgattta
      181 tcgttgggta caagagtaca gcaaagtcct ctattatctt tggaagaaga aaaatagaca
      241 atccttctat tcatggaaaa tggacgaaac ctatatcaaa attaagggac gttggcatta
      301 tctttatcgt gcaattgaag cggacggctt aaccttagat atctggttac gaaagaaacg
      361 ggatacgcaa gcagcctatg ctttcttaaa acgactccat aaatagtttg gtgagccgaa
      421 agcaattgtg accgataaag caccttctct tggctccgcc tttagaaagt tacagagtgt
      481 gggtttatat actaagacag agcaccgaac tgtgaagtat cttaacaatt taatagaaca
      541 agaccatcga cctattaaac gacgaaataa attttatcaa agtctccgta cagcctcttc
      601 cacgattaag ggcatggaga ccattcgagg aatatataaa aagaaccgaa gaaatggaac
      661 gctcttcggc ttttcggtgt ctactgaaat caaggtatta atgggaataa cagcctaaga
      721 tatttggagt tcagagaggg cgcgtttgat tttcaaactt cgcaacagaa ccttcatatt
      781 tccttgatta cctgaggcaa tgaaagactt atctacttga attagggtga aattctcatt
      841 caaatatggt agtaaaatta atatatgtga aaatcgaatc ttgagatagc tattcccctt
      901 gagatattca ctgatacttc tactcaaatc atttaaattt ttataattat ttgtacggaa
      961 cgtatatata tcaacatcag ttttctttac atgagaactt attctaaaat gaaaaagcct
     1021 actctcagaa agaattgaaa aatagatact ttcagtagat actgaagatt aaacaaaatt
     1081 aaacttgtat ccttcaggca tagcactttt aacagtctgc tgcattaact cggataatct
     1141 tttgaaagta gtaatatatt ttttaccagc aaaccataca tatctttttt cattaagtat
     1201 gttcactatc tctcctccat taattatatt tagcatcaaa tagttattgt acctatattt
     1261 tatcccattc cttttaaaac aagaatacgt acaaagtaaa atgttgtaaa tagtctagca
     1321 gggatgtata ttttcatgta ccaacataat atttaagaag tttcccatag atatattgga
     1381 cttcctcttt taaaacacaa aaataaatac taccttattt aggtagtatt tattaaaagt
     1441 tacgtcaatt ttgattaaaa atatactact tgaacctctc atgagtaaag tcacgaaatt
     1501 tttgggaata gagcgtactt gatatcttat tttttcacta atagcagtgt ctcttattca
     1561 ccaagcgatc cccgtagttc ctacggttct taatttttct ctagactaat gattgtatct
     1621 ttagaccttc ggccataaca tggagcaaaa taaaaaggta ctaagtctta tatatcaatc
     1681 ttcgagtaac cgacggttaa caaccatatc gagcggcatt aatcacccac ctgtagagga
     1741 tgagcgactt ttgcctaatt attgttaaaa gcctagtttc atttgtttag attggaaaat
     1801 acgtcctaca agtatctctt ttttttcatg gtcgatccga taaaataagg catagctttt
     1861 gccgatcacg atccgatatg ttgggatatc cattccatag atttcggaaa cttcctcatg
     1921 catagttgga aaatttttgg ttagttctaa agccttctgg atcgcaccta caaatctttt
     1981 tatgctggct ggagaaagag caagctcatt ttcccattct tctattatcg tttcaaggct
     2041 ggtctggaaa cttttagaat aaactagcgt gtactctttc atctattcac tccagccgta
     2101 ttttccgaaa gcttttacca tttcaatttc tgaaactgtt aaacctttct caaagtcttg
     2161 gaaactttct tcaatcaatt tgcgatcgtg ttcttttgct atttgagtag tatctaatat
     2221 gaataagttg ccttcttgtt tggcttggat agtgtctccg ctttttaatc caagagagct
     2281 tggaaggatc actcccacgc tattgccgat ctttctaacc tttagttctt ttcccatgtt
     2341 atcacacttc ttttagtttt tgataaataa attataacat gttataacga caattgccaa
     2401 cggtgtttct taagggattc cactccatat tattattgga caaaataagc cacttagaac
     2461 gctgaaaaca tatacaaacg atatcaaaat acgagtgaac tactcaccat acacttccgt
     2521 taacatccat ttaagtaggt atttttatta tatatagatt taacatatgt cggtcggttg
     2581 attgatatag ctaaagttat aactcaaccg aagccgattc atctacacga ataaagttgc
     2641 gagtgttctc ggctaattca taaaaattgt tatgtgttcc atatagcaat actggaataa
     2701 ctttattttc ttgaatctca taagcaactc tatacacggt gaccgcatac ttaaatccat
     2761 tagaccaatt acctgataaa cctccagttt tttgctctta cttaaatgga tcaatagcaa
     2821 tttcatcaca aacctataga ggatgagtga cttctgccta attacgttaa aaaatattga
     2881 aagtaactct atgtagcaaa aaatatctag aatttcattt cctcaaaact gatcgatatt
     2941 gtatctaaaa atatatacta cctaaaatag gtagtatata ttaaaatatg tgcttttgtt
     3001 agaaaaatca atacataaat tatcatatct gtatatgtca tttaggtatt tcagttatgt
     3061 tataatagtt ttgctgtctg ctaccccggc ctaatctggc aaggggagga ggagatataa
     3121 tgaaatacct ttttgatttt gtttttgcaa tcaagccatt tgcagaggta actgcacact
     3181 acatctgcaa gtggcttgat tgcaaaattg ccaaaaaaga cagtaagcct accagttaat
     3241 tctataatat agaaacgtaa aaaaaaaccc catcagccta cacgctgatg gggtttgatg
     3301 atataatgaa atacctcttt gttactcat
//
LOCUS       DACDEG010000158         3261 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.164, whole genome shotgun sequence.
ACCESSION   DACDEG010000158 DACDEG010000000
VERSION     DACDEG010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.164"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..860
                     /locus_tag="IV142_002685"
     CDS             <1..860
                     /locus_tag="IV142_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8486265.1"
                     /translation="WKLHKYICKNEFDIVIDIDTLLSLYTTRAIKGTKCKLISWEHFN
                     YWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDEYSLSEDFVITMPNPCISN
                     IEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDDWDLIIVGKGELEKDLRQQ
                     IKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMVILEAQSYGLPVISYDCKT
                     GPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRMSLNAYNNVQKFNLKEITK
                     QWVALIENV"
     gene            996..2528
                     /locus_tag="IV142_002686"
     CDS             996..2528
                     /locus_tag="IV142_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8486266.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            2706..2927
                     /locus_tag="IV142_002687"
     CDS             2706..2927
                     /locus_tag="IV142_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAP8486267.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
BASE COUNT         1148 a          340 c          628 g         1145 t
ORIGIN      
        1 tttggaaatt gcacaagtat atatgcaaaa atgaatttga tatagtaatt gatattgata
       61 ctttgttgtc tttgtatact acaagggcaa ttaaaggaac aaagtgcaaa ttgatttctt
      121 gggaacattt taattactgg acaatggaaa tgctgaaaga aaagaagaga ttcaaggcta
      181 aaaaattgat aaaaaaatat gcaaagaggc ttgtggttct cacagaagaa gatcgtcaaa
      241 aacatattga tgaatatagt ttatcagaag attttgttat aactatgcca aatccgtgta
      301 tatctaatat cgagtacgaa tatcattatg ataaaaagag atttcttgca gtagggcgat
      361 tagtggaaga gaaaaatttt gctgacatga taagtgcttg gaaaattgta gaaaaaaaag
      421 tagatgattg ggatcttatc attgtaggaa aaggtgaatt agaaaaagat cttcgccagc
      481 agataaaaga tttaaatctg caaagagtaa ctatagcagg gcattcagac catgtggagg
      541 aatattataa atctgcatct tgttatgtgc ttagttctgt ttatgaagga tttcctatgg
      601 ttatattaga agcacagtct tatggattac cagttatttc ttatgattgt aaaactggtc
      661 cgagagattt ggtacatcat aattttaatg ggatgttggt tgaagataag aatatagacc
      721 aattagctaa gagcatgata atgtttacta agaatactga tttagcaatg agaatgtcac
      781 tgaacgcata taataatgta caaaaattta atttgaaaga aattacaaaa caatgggttg
      841 ctttgattga aaatgtatag ttttttgtgg ttgtccaaac atgaaaatta gcgtaattaa
      901 tcaaagagct agaagaaacc tattacattt caagatttta gaaattatat agtattaggt
      961 aacaataaaa aatagaaagt ttggagccat atgatatgat aattaatcgt aaacaaaatg
     1021 ctaaaaggaa tataattact ggaacaattt ttaagttata tcagattatc attccctttt
     1081 ttatgcgtac acttatgatt tatcagatgg gaatggagta tgctggattg aacaacctgt
     1141 tcacatctat atttcaaatg ttaaatttag cggaattggg aataggtgct gcactaactg
     1201 taagtatgta taaacctata gcagaagaca atagaaaaga gatttgtgct ttactgaacg
     1261 catataaaaa gtacttttca attattggat ggatagtgtt tacattagga ttgttatgca
     1321 ttcctttttt gagattttta gttaaaggtt cgattcctgg aaatttaaat atgacagttt
     1381 tgtatttaat gtatttgata aatactgttt tgtcgtattg gttattttca tatagaaaga
     1441 gtctaatata tgcacatcaa agaaatgatg taattaacgt gtctatgcta attactttta
     1501 cattccaatt tgtaatgcag gcatatgttc tgattttctt aaaaaattac tatttgtatt
     1561 tattggcttc tattttggga caaatagttc ttaacttaat gtgtgctgtt gtttctcaaa
     1621 agatgtatcc tgattatact cctgttggtg agttagagaa agaaactatt gacgatatta
     1681 taaaaaaagt aaaaggatta gttacaaaca aggtaggcgg tacaatatta cgatcttcag
     1741 attcaattat tatttcatct ttcttaggtc tttcagtact tgcgatttat cagaattatt
     1801 actttattat ttctgccata atgagtatat ttgcaataat ttatgaatca tgtatagcag
     1861 gtataggtaa cagtcttatt gtagaaagtc aagaaaaaaa ttatatagat ttttgtgata
     1921 ttacaacaat tataggttgg tttctggcaa ttagttgtgc atgctttatt acattatttc
     1981 agccgttcat gattatctgg gtgggaagtg ataatcttat gagtatttca tttgttatat
     2041 ttatgatagt tcattttttc ttatatgaaa ttgatcagtt aattgggtta tacaaagatg
     2101 ctgctggaat atggtacgca gacaggttta gaccattaat tacagcaatg ataaacattg
     2161 tattaaatat catcctcgtt aattggttcg gattatatgg agttttggcg gcaaccattg
     2221 tttcagtatt gttggttgat ttaccctggt tattatataa tgttttccaa aatatattta
     2281 aatttgaatc ggcaggtaaa tatgtttgga agctcataag tttgtgtgta atagcttttg
     2341 ttgcttctta tttaagttat tttgcttgtt cgtatgttaa tatcggaaat ttcaaagtga
     2401 taagtttagt gataaattta gttatatcgg ttgtcatatc gattttagtt attcttctgt
     2461 ttaatatccg taataagaag tttaagtata ttttgaaagt tcttacgtcg gtattggtaa
     2521 aaagatgaga agagacatca gaatgatata taaagtaact acaattttta ggctgccgaa
     2581 taggcgaacg atgtatagtt tttgttccaa cgaagactct tgttgaaatt ggagatgata
     2641 tgaaggagtt acaattttga ctcatgttta tgattgatcg gttttaaaag tagtatggag
     2701 aagctttggg atcatcggag aggatcaaaa taggtaataa tgtttttata ggaatgaagt
     2761 ctagaatact aaagggtgta catataggaa ataatgtagt tataggtgca aatagtttag
     2821 taaacaagga cgtatcagac gattgtgttg ttgcaggaaa tctagcaaaa gtaattatgt
     2881 ccctagatga atattataaa aagataaaca atatgaagaa tccgtaaaac ttgtgaaact
     2941 ataccgagaa taatatggga aaaatattga tgaaaaagca ttatatgagt ttttagggct
     3001 gtttacagat ggagatgaca atttgtcgaa gagttgggag caatgcaaaa tttagtaggg
     3061 gattataaac agactgacaa ggtatttaaa gaacatgcac aagtatttca taatattgaa
     3121 gaatttttga atactgtgag ataggagttg ctacattgag aaaagtatgt atttttccaa
     3181 tgtaaaagat tacgagtttg aaaacgacac cataatatta ttttttaaga agatattata
     3241 gagcttagga acggaagttt a
//
LOCUS       DACDEG010000159         3219 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.165, whole genome shotgun sequence.
ACCESSION   DACDEG010000159 DACDEG010000000
VERSION     DACDEG010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.165"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(710..1666)
                     /locus_tag="IV142_002688"
     CDS             complement(710..1666)
                     /locus_tag="IV142_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486268.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(1775..2020)
                     /locus_tag="IV142_002689"
                     /pseudo
     CDS             complement(1775..2020)
                     /locus_tag="IV142_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2165..2587)
                     /locus_tag="IV142_002690"
     CDS             complement(2165..2587)
                     /locus_tag="IV142_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486269.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(2726..3156)
                     /locus_tag="IV142_002691"
                     /pseudo
     CDS             complement(2726..3156)
                     /locus_tag="IV142_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347687.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
BASE COUNT          973 a          702 c          533 g         1011 t
ORIGIN      
        1 gtgaggtcgt aaacaaagat tgaactatct acattcatac tctataatct gtttatttgc
       61 agactgataa tagcaaaaga aaacttataa tcaaataata tcaacatttg atcacataat
      121 ttgagattga atgctgattt acctatgttt tacgtaatgg tctttagtta tttttgctca
      181 taaaatgcat aaaacacgcg tttttatgtg tgcttgaaat ttcttggctc tataatttct
      241 aagactgata aaaatattct atcggttctt tttgcaaata aaaaaacata tagaagctcc
      301 attagattga atcaccacaa cacaatcaaa ggagcattcc gtatgcttga ttttcattct
      361 ataaaactac ttttaggaat caaagaaaat cacaacggtt caaactaaaa gcaagaatat
      421 ccgaagtacc attgtttcat cgatcatgag aaaagcattc acctctgtct gcagatgttc
      481 ttatatcgct ctgtcagtcc tattaataaa gtactgtttt gttttcaaaa aaactattac
      541 gagtactttt taagagaatg ccttgcaaat attcatcaca caccgttata taagtcaaat
      601 agtttctttc agtcgtcaag aaaagattct cctgtccttg gcattatcag ttaagtagca
      661 acttaattct cggacttgtt ctactctagc aaaatttatt ttcatcaact tacgtccaaa
      721 tgtttttttg aataattact tcatcgcggg tctggaagac ttgcctcatt cgataagtca
      781 ctagcttttg gcgtccttca ggatttctag tatacaccaa ctgacatttg ggcagcgacg
      841 attgatttaa ttcgtttaga aaaaggttag cgatttgctt atttcggcca aagcgttctg
      901 gtaccgtcag aactatcttt ttcccttcga ttaaataccg cggattctcc agcggtgcta
      961 aaagctccct catagcttca gtaaaaagct gtccttctcg atagccagca ttctcaagat
     1021 agcatctaaa ttcctgctga tcagccgcta cgattacttt tctatcacta acttcaccaa
     1081 ggcgctgcaa cgtcgtttga acgcatatgg ccaagctttt tttgcgggca cattcctttt
     1141 cccatagctt ttgtcgccta cgaatttgga aatattccag caagttacct ttaaatatag
     1201 ctgaccagaa gcttagaggc tctatatttt ttactactat cggcaatgaa gtttcttgtt
     1261 tttgctttac ctgatcgact ttcctaacga cgattggcat agataggtgc cagccatcag
     1321 ctaaagcagt actccattgt tctttcaacc gacttcgttc acaagaaaaa tagaacatcg
     1381 actgagtata agtttccatc gtggtggtcg tatattccag tggtaaagca aagcgatcgc
     1441 caccactctc aatttttggt gaagattcaa aggtcagacc tgaaatattg cccaaacgcc
     1501 gtagcattct tttcaaatca ggctgttcag acagaggaac tcccggtaat ccaatcgcta
     1561 aatgccaaat gttcgttact ttattttgtg cacctaggcg taagccccgc cctcgtagtt
     1621 gttgtgtttg cacaaaagtg gaagcttgat tagccaagat caacagatta attgcaggac
     1681 aatcccagcc ttctcctagc aaactaacgg ttcctaccaa aatattcaac ttcttttcgg
     1741 ttaataggcg agtaactgct tgcatcaatg cttggcggtt tttgtcatcc accgtcaaat
     1801 aaaaataacc cgacatataa ggatgttcat tcatacccgt cgctagttct gccagctcaa
     1861 cagttactag cattttcggt aacagtaaga actctccaca gatggcaccg atttgctgag
     1921 tgttacctaa tagacgaaca acttcatcaa acaaaggaac cacgccataa gctaaggtac
     1981 catcactgcc tgctaatacc tccttgcaaa tacgatccat caaaatgacc gctgtcagtt
     2041 cttgcttcag ctgaacttct tcttttaaca aaatttgaca aactgcttgc tttttctcag
     2101 gaatcgcctg actaccaaca gtgtctggat actctggaaa caaatgcagc gactcatcaa
     2161 acaatcatcc agcttttttc aattgataaa aaataggcaa ttccggttga tcttcaaaca
     2221 tctgctgata aatagtgatc agctcttttt tacctaaaga tggaactgaa gcttttttac
     2281 cttgctgctt agaactgcct aatagctgcc aatgttcggg tatcaattca tagttatgat
     2341 gattcaagta aattaatgca ctagtgaaca gctcaaaatt ttcataaatt tccttcgaat
     2401 gctcaagcgg atgctgaaca aattctaaag aaagcaaata atccaccgtc tcttggcttt
     2461 ccattaacgc ttccaccagc tcattttgtt tctctaaaaa ggtaaaataa tgctgagtag
     2521 tttcctgatt agccggaatc aaatatagaa aatcctgatg aggacataga acctcctcct
     2581 ttaccaactc gggaatgcta atttcttcgt caatcaggcc attttaactc aatatattgc
     2641 ttccactccg tctgagagct atcaatcgga ggcgtggctg tcaaggaaat cgtcaacaga
     2701 ttcggttgtt ttttcttcaa gtcaatcaac aattcacccc agcttttttt taaatgatga
     2761 gcttcatcaa aaaactaaca gctccagctg atcaccataa gttggctcct ccttcagtcg
     2821 gttgtaaatg gtctgatacg tttccactgt aatatgacta ggatgttcaa tcgccccaga
     2881 aagatagcaa ctatctgctg gatttactaa gaaatattcc tcgattgttg ttaaccactg
     2941 tttcttcaat attagggaag gaaccaaaat taaggtttta caatccaaac gtcggatcat
     3001 ctcaattcct aaaaaggtct tgccagcccc tggtggagcc actagatgga ctgaattgtt
     3061 atttaaataa gtagcaaagt tagtcaatat acgcttctga tagctacgcc actgaatttt
     3121 ttcatcagat agccacatcc caggtccccc tctcacgtat tttctctatc ataatctaaa
     3181 tgaatacagc aaaaaaaaga tttcttcaag aatatgtgt
//
LOCUS       DACDEG010000160         3188 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.166, whole genome shotgun sequence.
ACCESSION   DACDEG010000160 DACDEG010000000
VERSION     DACDEG010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.166"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            185..481
                     /locus_tag="IV142_002692"
     CDS             185..481
                     /locus_tag="IV142_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486270.1"
                     /translation="MNVKIRDLDPKFISEIDRRCVELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQEKFDQLLEKFSEIQSYNTKILEEYVSQNNRIIEILIEQNRGSEL"
     gene            483..905
                     /locus_tag="IV142_002693"
     CDS             483..905
                     /locus_tag="IV142_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486271.1"
                     /translation="MAKVIDKHIYFHQTHIDVLDEIISTHAEVKNYSEAVRFLCMNYD
                     PEVEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGKNSTVYQEAKERVE
                     GKIKSNQTKKYSPQLKKPVNESVHARVDPFDPTKILKR"
     gene            924..2516
                     /locus_tag="IV142_002694"
     CDS             924..2516
                     /locus_tag="IV142_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486272.1"
                     /translation="MTQSPSVILTCQFTLPNKKSFSTYIDYITREQALEEKERRTPEE
                     EQELQRIKNALEEFYIPEGETFSESKTGKEFSETSNEAQSLMEHGINFDKLEEQDFTN
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSIASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            2537..2872
                     /locus_tag="IV142_002695"
     CDS             2537..2872
                     /locus_tag="IV142_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486273.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVNGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
BASE COUNT         1260 a          471 c          637 g          820 t
ORIGIN      
        1 ccccactttt aaaaaaagtg gttggatagg ctgtgcctat cacgtttctg aaaaaaataa
       61 atgaccccaa aataccaata tataggatag tacaaggata gcaaaggata gtaaaaggat
      121 agaggtagga tagcgaaaag gataggatcc ttttcactat cctatattat attggaggat
      181 atttatgaat gttaaaatta gagatttaga tccaaaattc atttctgaaa ttgatcgaag
      241 atgcgttgaa ctaagtaatc gtacaggaaa taaatggagt cgtaatgatt atttaaagct
      301 actcgttgaa aatgattttg atcgtccttt gatggaatac aaacaagaaa agtttgacca
      361 gttattagaa aagttttcag aaattcaaag ttataacaca aagatattag aagaatatgt
      421 tagccaaaat aatcgtatta tagaaatctt gattgagcaa aatagaggaa gtgaactttg
      481 atatggcaaa ggttatagac aaacacattt actttcacca aacacatatc gatgtattgg
      541 atgaaattat ttctactcat gcagaggtta aaaactatag tgaagctgtt cgctttttat
      601 gtatgaacta tgatccagaa gtggagaaaa aagtaaattc caatcaagca aagttgaatg
      661 gaatgagtaa agaaatttct attttaaccg aaatggtttc agaatttgcg gatcttcatt
      721 tgaaagatac aggaatatta attggtaaaa attcaactgt ttatcaagaa gcaaaagaac
      781 gagtggaagg aaaaattaaa tccaatcaaa caaaaaaata ttcccctcag ttgaagaaac
      841 ctgtgaatga atctgttcat gccagagtag atccatttga tccaacgaag attctaaaaa
      901 gataaaaatg aaggaggata ataatgactc aatcgccttc agttattctc acttgtcaat
      961 ttactttgcc gaataaaaag tcattttcta cttatattga ctatattaca agggaacagg
     1021 ctcttgaaga aaaggaacga cgtacgcccg aagaagaaca ggaacttcaa cgaatcaaga
     1081 atgctttaga ggagttttat attcctgaag gagaaacttt ttcggagagt aagacgggta
     1141 aagaattcag tgaaacgtcg aatgaagctc aatcgttgat ggaacatgga atcaattttg
     1201 acaagttaga ggaacaagat tttaccaatt atctttctta tatgacacgt cactatgcgt
     1261 tagaacaaaa gaaagaactg acgcaaagtg aacaaaaaga ataccaacgc ttgaaccaag
     1321 caatgaaaaa atatcaaaca gaaccccata aaaaagcttc aggaaatctt cctggagttt
     1381 tttctatagc ttcagacgaa gtgaaaggaa gtgatctgaa ggagattaag caaatcttcc
     1441 aaagaggaca acaaaaaggc tcaatcattt atcaagatgt tgtttctcat gataattcgt
     1501 atcttgaaaa aataggattg tacgatccca aaacagatac tttagatgaa gaagggttaa
     1561 aagcagctgg aagaaaaatg atggagacat tattcgagaa agagcaactg aacgaaacag
     1621 gctactggat ggcaacgatt caccgtaaca ctaaacatat ccatattcat tttgcagttg
     1681 ttgaaaaaga aaatacgcgt aaaccttaca cggaggttga aaatggtgtg agttatatcg
     1741 taccgaaagg taagagaaga caagcaacat tagacgcaat gaagaccaat tatgtacatg
     1801 agttagaacg tttttcttat gaaaaggatc gaatgattgg tatggagaaa cgaaatttat
     1861 tgacgcggaa aagtgatttg cgtaacacct taacaaaagt ggtaaaagag ccagcgcgtt
     1921 acgatcgaca cgcgatgaac ttattaaatg aagtatataa aaccctacct gacaaacgtt
     1981 cggaatggaa ttacggggat gaaaaacgaa ccaaactttc accatacaca agagagaaac
     2041 tcgatgagtt aaccaaatat gttttaacaa aagaacctga atatcaagaa tatatacaga
     2101 tttcaaaagc attgaaagaa gaagcaaaaa gtctttatgg ggaaagcaag agagaatcaa
     2161 aggatgcaga gaaaaatgct ctctttgatt taaaaaagcg cacaggaaat gccatcctaa
     2221 cagagctgaa gagacaggat agtcaaataa aaccattaat agaaagtgtt gatcagatgc
     2281 aatctattta caatgaacga tcctttcgaa cgcttaaaca attgccaacg aatgaataca
     2341 aatatagtta cgaagaattc atgaagcgca ggaaacaatt ccaacaaaga gttgtcacgc
     2401 agcggagttt gcgtcaatta aatcgaacga ttaatgccca tgaagaaaaa taccacgcaa
     2461 gtaaagcata cgaagagcaa caacgaagaa ttcaaatgga acaagaaaga cagtaaagac
     2521 agtaggagga atagcaatga aacaatattt agatcaatgg aaagttatag aaggatcatt
     2581 aagagaagaa cgaatcgaac aacttcctga ttgtttagaa aaagagcatt tgttccagat
     2641 aagagaaatg ttgagaaacg aacagtttga tccaaatcaa tttttagtcg ttgagtaccc
     2701 agctactggt gtgtattgtt gtaatcatgt gaatggtgaa aaatatttta tcattcaaga
     2761 atatgagggc aagttagcac cttattatac tacttgggaa atgaatgaag agggaatcaa
     2821 taactttcca tgtaaaagca ttgaagaatc catttcactc acagagtgtt agttgttaag
     2881 tgaataaaag aagtaaaaga gtcctgaacg tgattgtcca ggactctttt tttcaatgaa
     2941 ttgttttccg ccaactcatt gagtggtgcg atttgcgact acattatatc aaaagaaaga
     3001 gaagggagga agcagaaatg gttcattcaa cgattggagt tgttgttgaa aaaagcagag
     3061 acaatctcgt ttttgtgaca gaaattcaaa caggcagatc ttttgtcgtc acagacaaag
     3121 cggcaaaagc ttatcaaagt agcgatattt taacgttaaa tctgactacg aagacgttcg
     3181 tcgatgcg
//
LOCUS       DACDEG010000161         3185 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.167, whole genome shotgun sequence.
ACCESSION   DACDEG010000161 DACDEG010000000
VERSION     DACDEG010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.167"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..344
                     /locus_tag="IV142_002696"
     CDS             <1..344
                     /locus_tag="IV142_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8486274.1"
                     /translation="TCIHKEKVASVKNNLKDKDLSTLLVLGKCFSDSSRIKIFYALET
                     YKEMCVCDLAAILNASIATTSHHLRFLKKNGMAKSRQYGKVVYYSLANEEILSAVQAF
                     LKLSENSSMKA"
     gene            361..972
                     /locus_tag="IV142_002697"
     CDS             361..972
                     /locus_tag="IV142_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HAP8486275.1"
                     /translation="MLQNILSALAVYISTSIDYLFILLIIFSQNHTKKGLRQIFFGQY
                     LGTGILVAISLFAAYVLNFIPQDWIIGLLGLIPIYLGIRVAFVGEEEEEEGEVVEKLG
                     SRGTNRLFWTVSLITIASGGDNLGIYIPFFTSLSFSEIVTSLIVFAISVAVLCYISYK
                     FAKISFVSETLEKYERIIVPIVFIGLGIFIMIENGTIQTILGL"
     gene            1000..2661
                     /locus_tag="IV142_002698"
     CDS             1000..2661
                     /locus_tag="IV142_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP8486276.1"
                     /translation="MSENKKYHEWILEGIDCANCADKVERGVAKVAGVANSSVNYMTE
                     TLSFEVTEEKEYLVLSNVQQKVKKLEPDITLKNKVDGRLIEINPALIKKSNLKDDKKN
                     YEWILEGIDCANCAAKVERGVATVPGVTNSSVNFMTETLSFEVAGENKTDVLASVKQT
                     VGTLEPDVVLRKKSDGTLIEHGTDSNKNSTSNKSISDKDETNQKQTNRAYLASLLGNK
                     TKITLIRLILGLGILATGIFLPMDGQASLGLFILAYLITGYDVVGRAIRNIFRGQVFD
                     ENFLMTIATLSAFYIQEYPEAVAVMLFYQVGEVFQDIAISKSRRSIADLMDIRPDYAN
                     LVTGNNVEKVAPEAIKIGNIILIRPGEKVPLDGKVVEGASAMDTSALTGESVPRSVKP
                     EDTILSGFINKNGLLKVVVEKPFSESTVKKILDLVQNASGRKAPTEQFITKFSRYYTP
                     IVVIAAALLAIVPPLLVPGATFNEWIYRASIFLVISCPCALVVSIPVGFFGGIGSASR
                     KGILVKESNFLEGLNDLKYVVMDKTGTLTKGFCCKVLNLLSNKVE"
     gene            2689..>3185
                     /locus_tag="IV142_002699"
     CDS             2689..>3185
                     /locus_tag="IV142_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8486277.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     MTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIGADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKASSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNL"
BASE COUNT         1032 a          496 c          666 g          991 t
ORIGIN      
        1 ctacttgtat acataaagaa aaagtagcta gtgttaaaaa taatttaaaa gataaagacc
       61 tttcaacttt acttgtatta ggaaaatgtt ttagcgattc ttctaggatt aaaattttct
      121 atgctttaga aacgtataag gagatgtgcg tttgtgattt agcagcgata cttaatgcta
      181 gtattgctac aacgtcacac catttacgtt ttttgaaaaa gaacggaatg gcgaaatcac
      241 gtcaatatgg aaaggtagtt tattactctt tagcgaatga agagattctt tccgctgtgc
      301 aggcttttct aaagttgtca gaaaatagtt ctatgaaagc ttagtttgga ggtagttatt
      361 ttgttacaaa acattttatc agcactcgct gtatatattt ctaccagtat tgattattta
      421 tttatcttgc tgattatttt ttctcaaaac catactaaaa aaggtctccg tcagattttt
      481 tttggtcaat atcttggaac aggaatcttg gtagccataa gtttgtttgc agcatatgtg
      541 ctaaatttta ttcctcaaga ttggatcatt ggtcttctgg gactaattcc gatttattta
      601 gggattcgtg ttgcttttgt gggcgaagag gaggaagaag aaggggaagt cgttgagaag
      661 ctaggatcta gaggaacgaa tcgtttgttt tggacagttt ccttaataac gattgcttct
      721 ggtggcgata atttagggat ttatattcct ttctttacct cgttatcttt ttcagaaatt
      781 gtcacttctt taatcgtctt tgctatctct gttgcggttc tttgctatat tagttataaa
      841 tttgcgaaaa tttcttttgt ctctgaaaca ctagaaaaat atgaacgaat cattgtacct
      901 atcgtattta ttggattagg gatttttatt atgatagaaa atggaaccat ccaaactata
      961 ttaggtttgt agagatctta ttagtaaagg aagcaaacga tgagtgaaaa taaaaagtac
     1021 catgaatgga tattagaagg aatcgactgt gcaaattgtg cagacaaagt agaaagaggc
     1081 gtcgccaaag ttgcaggtgt tgccaatagt agtgtgaact atatgacaga aaccttgagt
     1141 ttcgaagtga cagaggaaaa agaatatctt gttttatcca atgtgcaaca aaaagtaaaa
     1201 aaattggagc ctgatatcac tttaaaaaat aaagtggacg gtaggctgat tgaaataaat
     1261 ccggcattaa tcaagaaaag taatttaaaa gacgataaaa aaaactacga atggatatta
     1321 gaaggaatcg attgtgcaaa ctgtgctgcc aaagtggaaa gaggagttgc tacagttccc
     1381 ggcgttacaa acagcagcgt caattttatg acagagacac tgagcttcga ggtagcaggt
     1441 gaaaataaaa ctgacgtgtt ggctagtgta aaacaaacag taggaacttt agaacctgac
     1501 gttgttctgc gaaaaaaatc ggacggtaca ttgattgaac atggaactga ctctaataaa
     1561 aattcgactt ctaacaaatc gatttctgat aaagatgaga caaaccaaaa gcagacgaat
     1621 agagcttatc tggccagcct tctcggaaat aaaactaaaa ttacccttat tcgtttaata
     1681 ctaggattag gaatattagc gacaggaata tttctcccta tggatggaca agcctctctt
     1741 gggttgttca ttctggctta tttaatcacc ggttatgacg ttgtaggacg tgccattaga
     1801 aatattttcc gaggtcaagt attcgacgag aactttctaa tgaccattgc gacgttaagt
     1861 gccttctata ttcaagagta tcccgaagct gtagcagtca tgctatttta tcaagtggga
     1921 gaagtattcc aagatattgc tatttcaaaa tctcggcgct ccattgcgga cctaatggat
     1981 attcgacctg attatgccaa tttagtcaca ggaaacaacg tagaaaaagt tgctcctgaa
     2041 gcaattaaga ttggaaatat tattctcatt cgtccgggcg aaaaagtgcc attggatgga
     2101 aaagtagtgg aaggtgcttc tgcgatggac acttctgctt taacaggcga atccgttcca
     2161 agaagcgtga agccggaaga cactattttg agcggattta ttaataaaaa cggattgcta
     2221 aaagtagttg tagaaaagcc cttttcggag tctactgtga agaaaatttt agatttggtc
     2281 caaaatgcta gtggccggaa agcacctact gaacaattca ttaccaaatt ttctcgctac
     2341 tatactccaa ttgtggttat agctgctgcc ttactggcaa ttgttccgcc attgctagta
     2401 ccaggcgcta cttttaatga atggatatat agggccagta tctttttagt tatttcatgc
     2461 ccttgtgctt tagtcgtttc gattccagtt ggtttctttg gtgggattgg ttctgcttcc
     2521 cgcaagggga ttttagtaaa agaaagcaat ttcctcgagg gtttgaacga tcttaaatac
     2581 gtggtaatgg acaaaacggg tacattgaca aaagggttct gttgcaaagt tttaaatcta
     2641 ctatcaaata aggtagaata atagaaaaag atagtaggag gaatgacgat gaatcatttt
     2701 aaaggaaagc aatttcagca ggatgtgatt attgtagccg tgggatacta tcttcgttat
     2761 aaccttagct atcgtgaagt tcaagaaatc ttatatgatc gtggcattaa cgtttctcat
     2821 atgacgattt atcgttgggt gcaagaatat ggcaaactac tctatcaaat ttggaaaaag
     2881 aaaaataaaa aatcctttta ttcatggaaa atggatgaaa cgtacatcaa aattaaagga
     2941 aaatggcatt atttgtatcg agccatcggt gcagatggtt taaccttgga tatttggtta
     3001 cgtaaaaaac gggacacaca agcagcctat gcttttctta agcggttagt gaagcagttt
     3061 gatgaaccga aggttgtagt cacagataaa gcctcctcta ttacaagtgc ctttaagaaa
     3121 ctaaaagaat acggctttta tcaagggaca gaacatcgta ccattaaata cctgaataat
     3181 ttgat
//
LOCUS       DACDEG010000162         3115 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.168, whole genome shotgun sequence.
ACCESSION   DACDEG010000162 DACDEG010000000
VERSION     DACDEG010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.168"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            69..1733
                     /gene="alsS"
                     /locus_tag="IV142_002700"
     CDS             69..1733
                     /gene="alsS"
                     /locus_tag="IV142_002700"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP8486278.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            1746..2456
                     /gene="budA"
                     /locus_tag="IV142_002701"
     CDS             1746..2456
                     /gene="budA"
                     /locus_tag="IV142_002701"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP8486279.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            2586..3083
                     /locus_tag="IV142_002702"
     CDS             2586..3083
                     /locus_tag="IV142_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP8486280.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
BASE COUNT         1047 a          513 c          697 g          858 t
ORIGIN      
        1 ctgtatcaat tatttgtgat tatttctaac taataattaa aaataaagca taaaaaggac
       61 gatgttcaat ggacgaaaaa atgaaacaag gttcgaaatc tgtagtagaa agtttgatta
      121 atcatcatgt tgattatgta ttcggtatac caggtgcaaa aatcgatggt gtatttaatg
      181 aattagagga tcaaggtcct gaattaatcg tgactcgtca cgaacaaaat gctgcattta
      241 tggcacaagc aatcggacgt attacaggag agccaggagt agttatcgca acaagtggtc
      301 ctggtgctag taacttagct acaggtttgg taacagcgac agcagaaggt gacccagttt
      361 tagcaattgc cggacaagta aaacgaagcg atctattgaa gttgacccat caaagcatgg
      421 ataatgcagc actgttccag ccaatcacaa aatacagtgc agaaatccaa gatccagaaa
      481 caatttcaga agtgattgcc aatgcttatc gtatggctaa gagttcaaaa aaaggcgcga
      541 gctttatcag tatcccgcaa gatgtagtgg atgcacctgt ccaagggaat gtcatcaagc
      601 cattgagcga tcctaaatta ggttctgctt ctgcagatga tatccgttac ctagctgaac
      661 gcattagaga agcaaaacta ccggtactat tagtaggtat gcgaggatca agtgaaaaag
      721 aaacgttagc catcaggcaa ttagttggaa agacagcatt gcctgttgta gagacattcc
      781 aagcagctgg agtgatctca cgtgaattag aagcgcattt ctttggtcgt gtgggtttat
      841 ttagaaacca accaggagat atgcttttga aacgaagtga tctagttatt gctattggct
      901 atgatccaat cgaatatgaa gcaagaaact ggaatgcgga aaaagatgcg cggatcattg
      961 tgatcgatga agcgccagct gaaatcgatc cttttatgca gcctgaaaga gaattgatcg
     1021 gtgatatctc agccactctt gatttattga caggttcatt agagcctcaa caagtgtcag
     1081 aagatgcaaa agaatattta gcatctcttc aagcagaatt aacggagcgg gacattgttc
     1141 aatcaaaagg agaagcaggt atcttgcatc ctttagaagt tattaacacg cttcagtcaa
     1201 aagtaacaga tgatatgact gttaccgtag atgtaggtag tcattatatt tggatggcac
     1261 gccactttag aagttatgaa cctcgtcatt tgttattcag taatggtatg caaacgcttg
     1321 gtgttgcatt gccatgggcg atttctgctg cattggtgcg tccaaataca caaatcgtat
     1381 cagtttccgg cgatggggga tttttattct ctgcccagga tttagaaacg gcggtacgta
     1441 aaaaattgaa tatcgttcat ttgatttgga atgatggaca ctacaatatg gtagagttcc
     1501 aagaaaaaat gaaatatcag cgggcgtcag gtgtagactt tggtccagtt gattttgtga
     1561 aatatgcaga agcttttggc gctaaaggaa tccgagctac aagtgtagag gaattagaaa
     1621 aagctttaga agaaggattt gcgacagaag gacccgtaat tattgatatt ccgattgatt
     1681 accgtgataa tgagaaattg ggagaaacaa tcttaccaga tcaattctat taaggaagtg
     1741 tcgacatgac tgaaaaaatc ttataccagc acgggacatt aggagctttg atggctggct
     1801 tgatggatgg aacagaaacg attgctcata tattagaaaa aggaactctg ggattgggaa
     1861 cattgcatgg gcttgatggt gaagtcatct ttttagatgg cgtagcttat caaggaagat
     1921 cagatggctc agttgcccaa ttagatggaa gtgaacttac cccctatgca gcaattacag
     1981 attttacgcc agatactagt ttttctgtct caaaaactgc agatggagaa caattgaaaa
     2041 aagacatatt agtaagagaa gccggggaaa acctattttt ggcagtgaag ataactggat
     2101 tattcaaaaa tatgcacatc cgtatcatgc cgaagcaaga aaaaccttac agaagactag
     2161 caaaaatttc agagtctcaa ccagaattcc aacaaagcaa tgtccaagga acattagtcg
     2221 ggttctttac gcctgaatta ttccaaggag ttgctgcagc gggatttcac ttgcatttta
     2281 tcgatgatac acatacattt ggcggacatg taatggattt tgaagtggct gaaggaaaag
     2341 ttgagattag ccgcatctcg tcccttgtac agcattttcc agttgatgat ccgacttttg
     2401 ttcacgaaac gattgattat gctgatttag cagcagaaat ccaacaagca gaatagaaaa
     2461 agacagcgag atgcgttcaa ggtgattaac ctgacgtatc tcgttttttt gaggaggaaa
     2521 cctagcgttt acaaaaaaac tgagctataa tcttctccat agatcaaaaa ggagcggttt
     2581 ttatcatgtg gacagaaaaa cagaaaaaag atgcatacga aattttatta ttacagcaac
     2641 aaggattatt agaagcagaa gataactggt tagcaaattt agcaaattca tctgcattgt
     2701 tgaatgagac tttgcctgag actgtctttg caggatatta tttattcgat ggtaatgaat
     2761 tgattttagg accgttccaa gggcgagttt cttgcacacg tatcaagatg ggaaaaggag
     2821 tttgtggaga atcagctgaa aaacaagaaa cattgatcgt tgatgatgtc aaaacccatg
     2881 caaattacat ttcctgcgat tcagccgcac aatcagaaat cgtagtgcca atggtaaaag
     2941 aaggtaattt aatcggtgtt ttagatatcg attgcggagt aacgaaaggg tatgatgaaa
     3001 tcgatcaagc atttctagaa aaatatgtag atcttttatt agcaaatttt aatataaatg
     3061 ctttttcatt tattaataac taaaatcgtc tattgaaaca aaaaagaaga gaaat
//
LOCUS       DACDEG010000163         3109 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.169, whole genome shotgun sequence.
ACCESSION   DACDEG010000163 DACDEG010000000
VERSION     DACDEG010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.169"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            47..736
                     /locus_tag="IV142_002703"
     CDS             47..736
                     /locus_tag="IV142_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP8486281.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            750..2252
                     /locus_tag="IV142_002704"
     CDS             750..2252
                     /locus_tag="IV142_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8486282.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            2265..2741
                     /locus_tag="IV142_002705"
     CDS             2265..2741
                     /locus_tag="IV142_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP8486283.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(2838..2948)
                     /locus_tag="IV142_002706"
                     /pseudo
     CDS             complement(2838..2948)
                     /locus_tag="IV142_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1025 a          415 c          612 g         1057 t
ORIGIN      
        1 tggagttgtt atccaaaaat aataaaaaat tggagtgttt aggataatgg ataaaaagag
       61 gtttttagat cttatttcaa aaggtttgat tgtttcctgt caagcattac ctggtgaacc
      121 attttacaaa gaggaagaaa gtttaatgcc ttattttgct ttggctgctc aaagggctgg
      181 agcagttggt atacgtgcaa attctgttcg agatattatt gaaattagaa agtgtgtatc
      241 attaccaatg attggaatta ttaaaagaga ttatccacca gaatctccat tcattactgc
      301 cactatgaga gaagtagatg aattagtaga aaccggtgtt gaggtaatcg ctcttgattg
      361 cactcttaga aatcgacatg atggactatc tataaattct tttattacta aaataaaaga
      421 aaaatatcct catcagttat ttatggcaga tatttctaca tttgaagaag gcaaaaatgc
      481 atataaagca ggaatagatt ttgtaggaac tactttaagt ggatatactg aggaaagtaa
      541 aaaacaagat gggccagata tagatttgat taaaaaattg gtagaagaag ggatttgtgt
      601 aattgctgag ggaaggattc atactccaga acaagcaaaa gagataaaaa aaataggagt
      661 tgcaggtatt gttgtgggtg gtgctattac tcgaccacag gaaattgcat caagatttgt
      721 taaagcatta agttaggggg agaaataaaa tgtttaaaaa attttcacaa ttaggacgcg
      781 ctttcatgtt accaattgct atattaccag tagccggatt actattggga ctgggaggag
      841 cgttaacaaa tgagagtgct ataaatgctt atccattttt agatcaaccg tggttgcata
      901 caattttaag tattatgagt tatgctggaa atgcagtttt tgcaaaccta gcactaattt
      961 ttgcaattgg tattgctgta ggacttgcaa atggagataa aggaacagct ggtttagctg
     1021 gtggagtagc ttatctagta tatactgcaa ctattagtgg gtttcttgaa cttttttcag
     1081 caaaagatgc aacactagat acaggtgtag tgggatcaat tgttgttggt ggtactgttg
     1141 catttttaca caatcgttat agaaaaattg aattaccaca attcttaggt ttttttggag
     1201 gatcaagatt tattccgatt gtttctagtt tagctgcaat cattattggt tctttctttt
     1261 atttaatttg gccaccaatt caaaatggtt tagtagttgc tggtgaacaa attgcccaaa
     1321 tgggtagttt aggaactttt ttatatggtt ttttattgcg tttaacaggt gctgttggac
     1381 tacaccacac catttaccct ttgttctggt atacatcttt aggtggtacc gaaactgtag
     1441 ctggatcaac aattgctgga gcccaaaata ttttctttgc ccaactagct gatccaaacc
     1501 acactggtct gtttacttat ggtactagat tttttgcagg aagatttgca acaatgatgt
     1561 ttggattacc agcagcatgt tatgcaatgt atcgtgcaat tcctaagaaa aatcgtaaaa
     1621 agaatggtgg gctttatttt agcggggccc ttacatcatt tttaactgga attactgaac
     1681 cagttgaata tatgttttta tttgttgctc cttggcttta tgtgattcat gcattcttag
     1741 atggattatc tttttatttt gctgatatct taaatattcg tgtagggaat tccttttcag
     1801 gtggtttgat cgacttttta ttattcggtg tacttcaagg gaatgacaaa actaattggt
     1861 taaaattaat tccttttggt attgcatggg catttatata tttctttgtt tttacattct
     1921 ttatcaaaaa atttaaggta gctattcctg gaatggaaga agaatcagaa gttcattctg
     1981 aaaaagtaat aaacaaaaat acatcattac atgaagagtc tcttatcatt attgaagctc
     2041 ttggtggaaa agaaaatatt gagtctgttg aagcttgtgc aactcgtttg cgtgttgctg
     2101 ttacgaatgg ggagattatt caaaaacaaa caataaaaga cactggtgca actgctgtct
     2161 tcgaagtgaa gggtggcatc caagctgttt ttggagggaa agctgactta ttaagtcaag
     2221 aaatcaatca aatattagga aaagataatt aggaggtttt taatatgttt gaaattttca
     2281 aaaagaaaaa acaagaaact ttttatgcac cttgttccgg aaaaatcatt tccatcacag
     2341 aagtaaatga tcctgtcttt tcacaaaaat tactaggtga tggttttgcc gtcatacctg
     2401 atggttcaaa aatttatgct ccattggctg gtcgagtaac aaatatattt cctactaaac
     2461 atgctattaa tataaaaagc aactcaggca ttgattattt gattcatatt ggaattgata
     2521 cagttgaatt acaaggagag cctttttcga tttgtgttca agaaaatgat gaggttactc
     2581 ctgaaacctc gttggttgat gtggattttg acaaaataat aaaatctggt aaagatactt
     2641 ctgttatagt tgtttttccg gagaaaaatc agttcaatga tttaataata gataatggtc
     2701 gaattgctca tgggaattca tgtggaaaaa tcacttttta aaaaagtgta tataaaaagc
     2761 tataaaataa gctaaatagg atatttgtca aatgaagttg gtgaacagaa agaactgttt
     2821 tctttctgtt cattttttta ttttattagt aatgattccc catgccaaat catgatccgt
     2881 aatttaaagt tataaaagct tcgaaatcca ttggcatttc gttggaatac cttgatatga
     2941 ttattcaaat ataaaatata gagagaagag agagagggaa gatatttata aagggcaaag
     3001 ttatgtttac tcaacttagg aaatagaaat agatcaattt atattctctt gtcaatcgaa
     3061 tatgaacctt agcactatat accaaatata gtaaaatgct gaaagatgt
//
LOCUS       DACDEG010000164         3024 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.170, whole genome shotgun sequence.
ACCESSION   DACDEG010000164 DACDEG010000000
VERSION     DACDEG010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.170"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(241..855)
                     /locus_tag="IV142_002707"
     CDS             complement(241..855)
                     /locus_tag="IV142_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8486284.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1461..1838)
                     /locus_tag="IV142_002708"
     CDS             complement(1461..1838)
                     /locus_tag="IV142_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486285.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2138..3019)
                     /gene="galU"
                     /locus_tag="IV142_002709"
     CDS             complement(2138..3019)
                     /gene="galU"
                     /locus_tag="IV142_002709"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP8486286.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          916 a          633 c          469 g         1006 t
ORIGIN      
        1 ttttattttt ctgacactac atattcttat aactcatttc tcataactgt gaaacaactt
       61 ataaaaataa caaactttaa acaaaacaag cttaaaatca aagatgatcc atcttaaaat
      121 aactaaaaca aaaaaagtag tcgttgttaa tcctcattaa taatcgtttt ctttttttca
      181 aacgctccat ttttatgtgg aacattattt ttttaaggaa ctttgtcaac atgtggataa
      241 ctattttcca cttgtgtcct ggactagctg ttttgctgta aaatacaaaa cttgctgcag
      301 caaaaagatc gttgcaagag aggctgttcc gcagatcgct ggatctagat ttggcaaagt
      361 caagactgca tcatgtaaat tggcgtggtt gtccaaaacc aaatcagcaa attcaaataa
      421 tcgcaaacca ctgctatgtt gcgatttgat acttcgtgac aaatcaaatc cagtaacaat
      481 gattaatgga tgaccatttt ctttgatcca ctgagctagt tcgatgattg ccgggtttct
      541 tccttcattc gaaagaagaa gaaaaatgtc ttctgatttg acagaaagct gatcaataat
      601 tgcctgtcct gcaccttcta gcgtttcata gacacctccg gctggatttt caatcaatat
      661 acttggtaca agatccgggg tttgatccca aaattcaaat gctacaccac ttagtgtgcg
      721 gctagcatat atctgcaatc taccaccttg attaatttgt ttcgcgatcc aagtgccagc
      781 ttgaagcatc agcggctctt ctatccttaa tttctcttcc aattggtgat aatagtcaaa
      841 ataacataaa ctcatttatc tctcccgcct agctatactt gacatgaata gaaacagctg
      901 cctaatctaa ggaagtacat aaaaaaacag tagttagaca gtaacacatg tggtctttgc
      961 tgtttcaaac acaaaaaagg cattgaacga atacgcccaa taccttgaca tagcaaataa
     1021 aacaacgtgc agaccatgga aaatatctac ataggggtag aattttcacc ctagattagg
     1081 tacaataaat taggtcgatg cactgcattg actatggcaa aatgatcggt cagggtgtat
     1141 gtgtgtgact ggtcattccc tgattcttag cgccaactaa gggtcaggga gccttttttt
     1201 attcagctgt ttctatctga atttattgta agtaatctct tatccaagtg caagcaagaa
     1261 aatacaaaaa aacattggta tgatttatat atatataaat aacataaaaa aaggaatggc
     1321 ttatcccgat acaacaagca ttcctttaaa aaagattctt ttatataagt gaaaaaagct
     1381 tttttccacc aattaaataa atctctcatc tcaatagttt atccattatt ttatcttttt
     1441 ttgcgtatac ctactaaata ttaccgtctt cttctcgttc ttctactttg ttcgagagaa
     1501 tctgtactag aaaaagtagt tgtatgtcta ccttttctgc ctatccgaga ctttcttttc
     1561 atatatggga tataaatgat tagatacagt acagcaaaaa ataaagcaaa tacgcaagct
     1621 tcccaactac ctaacgccaa ttcaaccatc aaggtagcaa ccccaacgga tgctgcatac
     1681 cagataaaaa tcagaaacgt ttcttttttt ctgaccatgc gaaacatgat aaatagacta
     1741 ataagcaata gtggcaacac aaatatccaa ttgcttcctt ccatcagcca cttgagtata
     1801 tcaagcagcg actgtgcaac agcgactaat tttcccatag tgaacacaca ccttttttta
     1861 taattaaaaa attcgccttt ttattctact atgaatcttc tggcttggct ttcatttctc
     1921 ttatgtaatc attttctttt atacaactta aaaaaaaatt aaaccattag cagaatattt
     1981 tctgaatcta ttcacgaaag tctttttcta tctttcgttt tccacgactc aactgatcta
     2041 gagtcaaaaa actaaaacga cagttgcgca aatgacgcac aagctgccgc tttaataaca
     2101 aatagccttt cattctcagc aggccactta tttccagtta actttctgtt cttagctttt
     2161 tactcaactg gataatatat gcacttaatg aagtcccaat ttccgaatgt tgaagtccat
     2221 attcaatatt tgtcttcata tatcctaact tatcgcctac gtcgtatctt tttcctttaa
     2281 actctctcgc atagaccgcc tctgtctggt taagagtctg aagtgcatcc gttagctgta
     2341 tctcgtttcc tgctcctggt tcttgagttt ctaatatctc aaatatttta ggagtcagta
     2401 gataacgtcc gataatggct aaattacttg gtgcttttcc catttcaggt ttttctacaa
     2461 acccttttac tctgcaacga cccttttcgc tatctgtttt aggatcaata attccgtatt
     2521 tagatgtttc attttctggt acacgcatga cggcaacagc ggaagcttgt gtttcttcat
     2581 aatccatcat caattgttga gtcaatggga tatcatcttt catcaaatca tcccccagca
     2641 tgacgacaaa cggttcattt cctataaatg cttttgcttg aagcacggca tcccccagtc
     2701 ctcttggatg agattgacgg atgaagtgaa gattgacttg agtcgtttct tcaactaaag
     2761 cgagtaattc tgttttttcc ttttcccgca aattgttttc caattcgata tttgcatcaa
     2821 agtgatcttc aatgggtctt ttttcttttc ccgttacgat caagatgtct tcgattcccg
     2881 aatccaatgc ttcttccaca ataaactgga tcgttggttt atccacgatt ggaagcattt
     2941 cttttgccat ggcttttgtt gccggcaaaa aacgtgttcc tagcccagcg gcaggaatga
     3001 ctgctttttt gaccttcact tgct
//
LOCUS       DACDEG010000165         3014 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.171, whole genome shotgun sequence.
ACCESSION   DACDEG010000165 DACDEG010000000
VERSION     DACDEG010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.171"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..412
                     /locus_tag="IV142_002710"
     CDS             <1..412
                     /locus_tag="IV142_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8486287.1"
                     /translation="LDLENMFLRVGVQAKAVLDPHFQAQVASLLTVNDLVIIFSLSGK
                     TKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQTAIEEFLNGGSLAGKISQLY
                     ICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            642..872
                     /locus_tag="IV142_002711"
     CDS             642..872
                     /locus_tag="IV142_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAP8486288.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(1095..1193)
                     /locus_tag="IV142_002712"
                     /pseudo
     CDS             complement(1095..1193)
                     /locus_tag="IV142_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(1458..1913)
                     /gene="tnpA"
                     /locus_tag="IV142_002713"
     CDS             complement(1458..1913)
                     /gene="tnpA"
                     /locus_tag="IV142_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP8486289.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2156..3014
                     /locus_tag="IV142_002714"
                     /pseudo
     CDS             2156..3014
                     /locus_tag="IV142_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          977 a          539 c          526 g          972 t
ORIGIN      
        1 tcttgattta gaaaatatgt ttcttcgcgt aggagttcaa gcaaaagctg tactagaccc
       61 tcattttcaa gcccaagtcg cttctttatt aacagttaat gatttagtaa tcattttttc
      121 tttatctggg aaaacgaaag atacttatga ttcattgaaa atagctaaaa aaaatggcgc
      181 aaaaattatt gctataacta actatattca ttcacctata ggaaagtctg cagacttagt
      241 tttacaaaca gccattgaag aatttcttaa tggtggatct ttagctggaa aaatatcaca
      301 actttatatt tgtgatctat tagttcatgg ttatgaacaa aacaataata ttgattcagt
      361 tgaattaaga gaaaaagttt tgcgctctat tattgataaa agtattgagt gaactacccc
      421 cacttagcta aacctaacgg tttttaacga ttgaagtagg caattcttgg gatgagagca
      481 tacttgatag cttatttttt actaatagcg gtggctctta ttgaccaaat atccctgtag
      541 ttcctacggt tcttgatttt atctaaagat atttttatcg tggaagctat tgatcaactt
      601 gaaagaaaaa cgtgtagctc attatcacta gtttatcgag tatggatgaa gcaatggatc
      661 atcctctctt aaataaattt attaaggatc tgatccttca aattttttcc atgatcgatg
      721 agcaggaacc tactgaatct aaacgacatc gggcacaagg aattattaag attgctaagc
      781 gaatgttttc tataaagggt gttctaagct atacagtgtt aattcaaaag attcccaatc
      841 atcttgttta taagaacaac gttgaagatt aaaaaagtgt ttcaatgcta aaattgcaaa
      901 atacttcaat gttatgagac aaactgtcta taagaatcaa tttccaccca tttcatcact
      961 tgttaaaagt ttatgatcct tttgattttt atattaattt atctgtattt aatacagatt
     1021 ttatttatta gttcatctgc ctattgtatt ttattttgtt gccttaccat ctgaagtaaa
     1081 tatactctta attgtcaaat taagttagac agatcatcaa tacttacgta actcaaaaat
     1141 acttccaaag gtgttcgata atccagtgat tttctaggaa gattatttct gcgccagcat
     1201 tttctaatgt ttgaatttgt gttttgtaat cttggttgct tgtgctaatt cttgaatagc
     1261 cgtaaattgc caaaatttca tcaccctttc tggacataag tttctgacac cagttaaaat
     1321 caagtctatc agtaacccat acacccgtga ggacgggtgt atttttaatc cccctagaag
     1381 gggtcgacac tcgctgcccc ttaaaagggg atttttaaaa gaccgccaac tgctcatctt
     1441 cactcactta cttctcttta ttttttaaat ggttctacgt actctctttt acttataata
     1501 tatcgaatgc gatcatcagc ttcttgttca cggatgtatt tttcatttgt cttttgattc
     1561 aatccgactg tactcacata gtatctcttc gaccaaaagg atttgtttcc gtatttatat
     1621 ttgagatttg cgtgctgctc gtggattagt accgcactac gacctttcag ataccccata
     1681 aaactggata tacatatttt tggtggtact cgaacaagca tgtgaatatg atctagcatt
     1741 gcatgtgctt ctatgatttc cacatctttc atttcgcaca acttcctaag tatttttcca
     1801 agatcttttc gtaattgccc atagattact tttcgttgat attttggtgt aaaaataata
     1861 tgatacttac aattccaacg agtatatgct aaacttttat cgtcatttga catgaaaaaa
     1921 ctcctttcga tctgatgagc ggttggtagc cactcactag tatatcgaaa ggagttttta
     1981 tcgctaaaga tacttcttcc acgcgtagaa acgcgcggtt ttcttgtttc gcacgcttta
     2041 cgcacaaaac tgactgaagt cattaagaaa ataaaagggt ctaaaacttt taagttttgt
     2101 accgaccccc aaaagttaga ccaaaaaatc taacttttgg aggtcggtat tttttatggc
     2161 taagtatact tttgaactta aattaaagat cgttcatgat tacttggatg gtaaaggcgg
     2221 aagtgattat ctggcaaaga aatattccat taaggctcca tcacaagtga agcgctggat
     2281 caatgcctat caagaatttg gtgaagaggg tctagtaaga aaacgacaaa agaaaaaata
     2341 ttctgttcaa tttaagctgg atgcgataga gttatatcta acaagtgagc tgtcctatcg
     2401 tgaagttgcc aacacactta acatgaacaa ccctaatttg atcgctagtt ggatgcgtca
     2461 attccgtgag ggtgggatcg atggactctc aaaaacgaaa ggatgtccac ctatcttgtc
     2521 taagaaaaat gaacccaaaa ataagaaaaa atctacaact aaagcaacgt ctaaagaacg
     2581 tgaacgcatc gaggagttgg agaaacgagt ccgttccctt caaattgaaa atgccttttt
     2641 aaaagaattg aggaagctgc gaaaacagga agcccaacaa cgacgaatga agcaatcgca
     2701 cgaatcatcg caagcctccg aggaccgttt aaattagttg agcttctaga aactttaaag
     2761 ttccctaagg caatctttat gtactggcaa aaacgattgg accgtaaaaa tccagatcaa
     2821 gagattgaag cggaaatgct taaagttcgg gagaagcaca aggattatgg ctgtttacgg
     2881 atgaccaatg aactaagaaa ccgcggtttc tctatcaata aaaagaaagt tcagcgcctg
     2941 atcaagaagc taggaataaa agtaacgaca tacacacgta aatcacggcg ctacaattct
     3001 tatcgaggac aagt
//
LOCUS       DACDEG010000166         2983 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.172, whole genome shotgun sequence.
ACCESSION   DACDEG010000166 DACDEG010000000
VERSION     DACDEG010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.172"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..2387)
                     /gene="esp"
                     /locus_tag="IV142_002715"
     CDS             complement(<1..2387)
                     /gene="esp"
                     /locus_tag="IV142_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAP8486290.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVS"
BASE COUNT          856 a          588 c          453 g         1086 t
ORIGIN      
        1 aagcttacgc cgtctttcac tgcaaagact gttgttccac tttcaatact tgttccttcc
       61 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
      121 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      181 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      241 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtcctt attatagaca
      301 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      361 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      421 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      481 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      541 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      601 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      661 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      721 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      781 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      841 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      901 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      961 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
     1021 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1081 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1141 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1201 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1261 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1321 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1381 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1441 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1501 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1561 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1621 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1681 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1741 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1801 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1861 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1921 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1981 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     2041 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2101 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2161 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2221 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2281 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2341 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2401 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2461 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2521 caacaaaaaa aagtacattt cttcatgttt ttttcggata tttgtgcttt ttaaccattc
     2581 agcagctgaa aacatataaa aagatatgta tgtattcttt ctgaaattaa gttcatttct
     2641 atgcgatgaa gcatgttttc aaaatattac cctttcttag attaaacttc agagtattca
     2701 aataacggaa taaggagcct tatcccgcat tactgaatga gactgtgaca taagtaaaaa
     2761 ggtgacttca aattccttct tcggaatttg aaatcacctt ctttataatt ataaagtatc
     2821 ggtttcacag catttctgaa tgattcctag aatcttaaac gaatgattgg agttatcaac
     2881 attttttcac tcgtttttag cgaaacaaaa aatgacattt gtcacagcct cattaaatta
     2941 ttgattcttt ttctttataa ttgaattgtc catatttaaa gtg
//
LOCUS       DACDEG010000167         2970 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.173, whole genome shotgun sequence.
ACCESSION   DACDEG010000167 DACDEG010000000
VERSION     DACDEG010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.173"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            300..917
                     /locus_tag="IV142_002716"
     CDS             300..917
                     /locus_tag="IV142_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8486291.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            958..1257
                     /locus_tag="IV142_002717"
     CDS             958..1257
                     /locus_tag="IV142_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486292.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1291..1458
                     /locus_tag="IV142_002718"
     CDS             1291..1458
                     /locus_tag="IV142_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8486293.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1503..2108)
                     /locus_tag="IV142_002719"
     CDS             complement(1503..2108)
                     /locus_tag="IV142_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8486294.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTVMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
     gene            complement(2368..>2970)
                     /locus_tag="IV142_002720"
     CDS             complement(2368..>2970)
                     /locus_tag="IV142_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HAP8486295.1"
                     /translation="SYREVQELLYDRGINVCHTTIYRWVQEYSKVLYYLWKKKNRQSF
                     YSWKMDETYIKIKGRWHYLYRAIDADGLTLDIWLRKKRDTQAAYAFLKRLHKQFGEPK
                     AIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKYLNNLIEQDHRPIKRRNKFYQSLRTA
                     SSTIKGMETIRGIYKKNRRNGTLFGFSVSTEIKVLMGITA"
BASE COUNT          958 a          507 c          549 g          956 t
ORIGIN      
        1 aaagaagtca aattctgatg tgaaaaataa aatataccat ggaagacata aaagaaaaac
       61 tggataacta gtttatttaa tggcagaccg ttaaggcctg ccatttttat gtattttagc
      121 ggaataaata taatcatgcg ggagtgtaaa cttactgttt caaaaagtat actttttgac
      181 atgccttgat tttctttgtt tatgtatggc ataataacca cataatacaa tttgaaacgt
      241 ttctgattta atctgattgt tttgaaacaa ttttagtgaa aaaagggtag ataaaaacga
      301 tgagaaaatt tttttatatc cgggtcagta gttatgatca atcgatcgaa cgacaactga
      361 tttccgcgaa ggaattagga atccctgaag aatatatatt tgtagaaaaa gccagcggca
      421 aagattttaa acgccctgaa tatcaattaa tgaaacggat gtttcgaaaa ggagatttac
      481 tctatctaca atcattagat agactaggca gaaataaaca aatgatttta gaagaatggc
      541 gagaattaac taaggaaaag cagattgata tttctgtgtt agacatgcct ctgttagata
      601 ctgctagtta caagcaacta gatgggttag agacgcttat ctctgactta attctgcaat
      661 tactcagcta tatggccgaa gacgagcgga aacgaattaa tagccggcaa agagaaggcg
      721 tagaagctgc gaaacaaaag ggtgtaaaat tcggcagaaa gaaaattgct ttacctcctg
      781 aatttcctgc gatctattct gactggaaag caggaaagat aactgcagtc tatgctatgg
      841 atcaattggg catgaaacga aatacctttt atcgacgggt gaaagaatat gaacatacct
      901 tgcaccactt ttcttgatag atataacagc tattagacgg gaagggagag tttgaaaatg
      961 gacttaacga atcaaagggt cgtttttact ggaacgctgc agcaaatgac acgaacccaa
     1021 gccatcggtt tggttcactc tttaaacggc cattgtcaat caagtgttac ttcaaaaaca
     1081 aatttcctgg tttgtggtca gtattctaag tcgttgtttg atgattctct ctatacaaaa
     1141 aaggaacaaa ccgcgcggga cttgattgcg ttaggtcatg agattaccat tttatcagaa
     1201 gtcgaatttt atgcgttaat tagtcaacag ttgaaagaat ggcaacggta tttataatcg
     1261 ttagtaaaat ttacaaggag gaaaattttt atgaagcgaa aatcttatga tcgttggggc
     1321 attgaaataa ctagttttaa tcgaaatatt gcttttcacg tggagtgtga ctgtggggaa
     1381 ttagcaaaaa gtgtttatca aaaaaattac ttttactgtc ctaaatgcca tcgccgatac
     1441 ggggtacatg gaacatagaa acgaaatagt gcggtagaaa agcgaatgaa tgtagtatat
     1501 ttctacaaat cattcgcttt ctttatttta taaatagttg gtcgagagat cccgttttct
     1561 tttgcaatct ctgcaactgg aatgccttgc tttaacatgt ccaccacggt tttaaaaact
     1621 aagcgttttt gcggatctgt tgagttaata gaatactcga ttggtcgtcc cttatatcgg
     1681 ccggcttttt gagccaataa tactccttgc cgctgacgtt cacgaatttt ctcacgctcg
     1741 ttttgtgcaa tataacttag cagtgataaa aacatatcga acataacggt atccaggagt
     1801 tcatttccag tattaaaatc taaaaactgt gcatctaata tttttaacgc tacttttttc
     1861 tgtttcataa aagcaactgt attttttata tcttcataat ctcttcctaa acgatctagt
     1921 gatgtaacga tgacgcaatc accttcacgg atataattta gtaatttttg aaactcagga
     1981 cgctcagtat ctttaccact ggctttttct tggaataatt tatcaatgtt aagtccttcc
     2041 atcattgcca attgtcgatc caaattttga tcttgtgaag atactcgaat atagccaatt
     2101 tttcccataa aaaactcctt tttaaaagtg atataattta catgtaaaat ttacttgtag
     2161 ttttactgct atcatagcat ttttaaaaat aaattttatg tagacttaaa atttacttca
     2221 tggagggttt aaatggatga aatcaaaagt aacaaaaaag catttggcat ttatttagga
     2281 gaacctagta cagaacaatt aaaccaattt tttggttctg ttgcgaagtt tgaaaatcaa
     2341 acgcgccctc tctgaactcc aaatatctta ggctgttatt cccattaata ccttgatttc
     2401 agtagacacc gaaaagccga agagcgttcc atttcttcgg ttctttttat atattcctcg
     2461 aatggtctcc atgcccttaa tcgtggaaga ggctgtacgg agactttgat aaaatttatt
     2521 tcgtcgttta ataggtcgat ggtcttgttc tattaaattg ttaagatact tcacagttcg
     2581 gtgctctgtc ttagtatata aacccacact ctgtaacttt ctaaaggcgg agccaagaga
     2641 aggtgcttta tcggtcacaa ttgctttcgg ctcaccaaac tgtttatgga gtcgttttaa
     2701 gaaagcatag gctgcttgcg tatcccgttt ctttcgtaac cagatatcta aggttaagcc
     2761 gtccgcatca attgcacgat aaagataatg ccaacgtccc ttaattttga tataggtttc
     2821 gtccattttc catgaataga aggattgtct atttttcttc ttccaaagat aatagaggac
     2881 tttgctgtac tcttgtaccc aacgataaat cgtagtatga caaacattta ttccacgatc
     2941 atataacaat tcctgaactt cacgatagct
//
LOCUS       DACDEG010000168         2857 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.174, whole genome shotgun sequence.
ACCESSION   DACDEG010000168 DACDEG010000000
VERSION     DACDEG010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.174"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            562..843
                     /locus_tag="IV142_002721"
     CDS             562..843
                     /locus_tag="IV142_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486296.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLEKFIAIQTKNTQVLNDYVHTNNQLIETLIGGK"
     gene            845..1261
                     /locus_tag="IV142_002722"
     CDS             845..1261
                     /locus_tag="IV142_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486297.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKT
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1422..1688)
                     /locus_tag="IV142_002723"
     CDS             complement(1422..1688)
                     /locus_tag="IV142_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486298.1"
                     /translation="MAFRFRCDRGGRKMTEKFPYEYYQNLEKLESAIQLVKLDLLDEN
                     LLKYKNITPERKGKLKKMQQLFDEEDGISDVIFDTWSEFHGEYN"
     gene            complement(1646..2620)
                     /locus_tag="IV142_002724"
     CDS             complement(1646..2620)
                     /locus_tag="IV142_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP8486299.1"
                     /translation="MGKKRTIVNEWEKLTAPSNRRLNVGESVKMIKMDWSIDRITIVG
                     TLNENIYYHTQNDVLILDFEQLMRVNEGNGHLKAVGNNGWQLVDQHEENIAYIEILKW
                     QEGKGRIDFNPNKISQFLASSMKKFIHDLFLEPHFSRADVACDLIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQEAKRKIVPKEVETWWRLELQ
                     LRRGKATDWYEMVQESLDSFASPHYFPEDVKTLDRVMIKGLLYDHSEWSTISRDLKYR
                     LRKLLKQESQNDELTNHLRETFEESADELKRELDTWLLGLDVTEEEEK"
BASE COUNT          926 a          624 c          397 g          910 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatcattt cacgcttccg ctcttcattt cattcagttc tcggctttca caatctaaca
      121 cgccgtttcg ctaccagtcg ccttacaggc tccattctcg cttctcttac gtgttttacc
      181 gattgtgccg acaactaaac gaaatgaacg ctaaaacgtc ttagaacgca aaatacacgc
      241 ttataaaatc atttcatctt gattatatgc tcatcataat cgttcaattt gacatcattt
      301 ccgtaaactt tttttatttt tataagcaga aaaaagtcgc acatcaacat ttttttaaag
      361 agcataagga aacgtgtacc cactttctaa aaaaagcggg cagataggct gtgcctatca
      421 cgtttctaag aaaaaaattt gatatcactt tttcgccatt gtgactagct tatgtatagt
      481 gtttcagcaa aaaacagcta tacaaatact atattcgtat gatatacata aattatacac
      541 aaatgcgaaa ggagtttttc tatggacatt ttagttcgga agattgattc aaaggttatt
      601 gcaaaaatag acaaacgttg ccaagaactc acaaataaaa ctggcaaaaa atggagtcga
      661 aataaatatc taaaagtttt aatcgaaagt gattttgacc gtcaacttac gacttacaaa
      721 aaagaccaat tcgatttatt actcgaaaaa tttatcgcta ttcagacaaa aaacactcaa
      781 gtactaaacg actatgttca tacaaataat caacttattg aaacacttat tggaggaaaa
      841 tagcatgaga aaaacttctg ttcaaaaaca cgttaccctt tatcccagtc acgtggacgt
      901 tttagattcc attatcgaaa gaaatccaga tatcaacaat tactcggaag ccattcgttt
      961 tttatgtatg agttatgaac ctgataccaa cacgactact accagcaatc aagccaaact
     1021 aaatgctatg ggaaaagaaa tttccatcat gtccgaaatc attgcagaat ttgctgattt
     1081 atatctaaaa gatacaggcg ttcttattgg cacaaattct aaagtgtatc aagaagtgaa
     1141 agaaaaaacc gaagctaaac taaatccgtc gaatcgaaat cgaccaaaga atcatcaaaa
     1201 agaaacgagt caaacagtca cttctagaaa tttcgaccca acaaattttc taaaacgcta
     1261 aaaaataaaa cgagaatttt tttatttctc gttttatttt ttaggaaagc cggtcttaca
     1321 gcaagatttg caagttaccg gtaacttgcg gaaaagaaga agccgctcac gcgacttctt
     1381 ctttttgcat tttctgaaag ctaccacaat tcctaacttc gctaattata ttctccatga
     1441 aattcgctcc atgtatcaaa aatcacatct gaaattccat cttcttcatc aaataattgc
     1501 tgcatttttt tcaatttacc ctttctctct ggcgtaatat ttttgtattt tagtaaattc
     1561 tcatccaaca aatccagctt gaccaactga atagcactct ccaatttttc tagattttga
     1621 taatactcgt atggaaattt ctcagtcatt tttcttcctc ctctgtcaca tctaaaccta
     1681 aaagccatgt atccaattct ctttttaatt cgtctgcaga ctcctcaaat gtttctcgta
     1741 aatgattcgt caattcatca ttttgacttt cttgcttcag caatttcctt agccgatatt
     1801 tcaagtcacg actaatagtt gaccactcag aatggtcata caacaacccc tttatcatca
     1861 ctctatccaa cgtttttaca tcttcaggaa aataatgcgg actagcaaaa ctatccaaac
     1921 tttcttgaac catctcatac caatcagtcg cttttccacg gcgcaattgt aactccaaac
     1981 gccaccacgt ctcgacttct ttaggtacaa tttttcgctt tgcttcctgt tccaacttct
     2041 tgttgtacat ccgaatttgg cgttcgctgg cacgactacc ccaataagcc gtttctagct
     2101 taccagagcg tccataaatt ggtttgaagg acacagggtc aactacacga tattgtgtaa
     2161 taaactcatc aggcacatca ataagatcgc aggcaacatc cgctcgtgaa aaatggggct
     2221 ctaaaaataa atcatgaata aattttttca tagagcttgc taaaaattga ctaattttat
     2281 tggggttaaa gtcaatccgc cctttaccct cttgccattt caaaatttcg atataggcaa
     2341 tattttcttc gtgttgatct accaattgcc aaccattatt cccaacagct tttaaatgac
     2401 cattaccttc gttcactcgc attaattgct caaaatctaa aatcaacaca tcattttgag
     2461 tgtgataata aatattttca ttcaaagttc cgacaatcgt aattcggtca atcgaccaat
     2521 ccatttttat catttttacg ctctcaccga catttaaccg cctgttagag ggggctgtta
     2581 atttttccca ctcattcaca attgttcttt ttttacccaa attttgttcc tcctcaaaga
     2641 aaaaaagaga tttttggaaa gaatttccag agttcacgta actctaaaaa tccacatcca
     2701 aaaatctcta gcttgaagaa acctaaaaat attcgtataa tgaacttgag ttacggaact
     2761 aggctagtag tttccgtaaa ggtcgagagt gttagcgcac tgctcgacta tttttttgct
     2821 cttttttctc gttttctttg attatagcac tcctctt
//
LOCUS       DACDEG010000169         2822 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.175, whole genome shotgun sequence.
ACCESSION   DACDEG010000169 DACDEG010000000
VERSION     DACDEG010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.175"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            95..1054
                     /locus_tag="IV142_002725"
     CDS             95..1054
                     /locus_tag="IV142_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS1252 family transposase"
                     /protein_id="HAP8486300.1"
                     /translation="MTYTHLTTDELVMIESYYHQNIKIVKIAEYLKRSRTPIYNVINF
                     LKEGHTALEYYQQYKENKKRCGRRNIVLPKKQQSYIKEKIAQGWTPDVIIGRKEKPIS
                     CSVRTLYRQFKEKVFDESTLPMKGKRKPNGHQERRGKQAFKRNIVERETDYPTFKEEF
                     GHIEGDTIVGIHHKSAVITLVERLSKIIITLKPEGRKAIDIENTLNHWFGSIPRHLFK
                     SITFDCGKEFSNWKALSNQHDIAIYFADPGTPSQRALNENSNGLLRKDGLPKNMDFNQ
                     VDQAFISSVANKRNNIPRKSLDYQTPLEVFMSYMNEDILSSLI"
     gene            complement(1487..2059)
                     /locus_tag="IV142_002726"
     CDS             complement(1487..2059)
                     /locus_tag="IV142_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8486301.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2075..2680)
                     /locus_tag="IV142_002727"
     CDS             complement(2075..2680)
                     /locus_tag="IV142_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP8486302.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          888 a          579 c          469 g          886 t
ORIGIN      
        1 gccgattgta aaattaagct agacaaataa ataagccatt cgtgctacac tcaaaatgta
       61 attccgacta aagaaaacat aggagtgatc acgaatgacc tacacccatc ttaccacgga
      121 tgaactggtg atgatagagt cttactacca ccaaaacatc aaaattgtga aaattgctga
      181 gtacctgaaa cgatctcgaa caccgattta taacgtcatt aatttcttga aagaaggcca
      241 tacggccctc gaatactacc aacagtataa agaaaataag aaacggtgtg gcagacgaaa
      301 catcgtcttg ccaaagaaac aacagtccta cattaaagaa aaaattgccc aaggctggac
      361 gccagacgtt atcattgggc gtaaagagaa accaatcagc tgttccgtaa gaacccttta
      421 tcgccagttc aaggaaaagg tgttcgatga atcgactttg cccatgaagg gtaagcggaa
      481 accaaatgga catcaagaac gccgaggaaa acaggctttt aaacggaata tcgttgaaag
      541 agaaactgat tacccgacat ttaaagaaga gttcggccat atcgaaggcg ataccattgt
      601 aggcattcat cataagagtg ctgtcataac tttggtagaa cgtctatcga aaatcatcat
      661 caccttgaag cccgaggggc gcaaggcgat cgatattgag aatactctga accactggtt
      721 tggatcgatt cccagacatt tatttaaatc cattaccttt gattgtggga aagaattctc
      781 taattggaag gctttaagta accaacatga catcgctatt tatttcgctg atccagggac
      841 tccttcgcaa cgagcactga atgaaaattc caatggcttg ctgcgaaaag atggtctgcc
      901 aaaaaacatg gattttaatc aagtcgacca agcttttatc tcttcagtgg cgaataagag
      961 aaacaatatt ccacgaaagt ccttggatta tcaaacaccc ctggaagtat ttatgagtta
     1021 catgaatgaa gatattttgt ctagcttaat ttgacaaatc aaattattta aaactttgca
     1081 acagaacctt agcagcagtg ctgattttta tgatagttat ttggttctta aatgaaagat
     1141 ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca atagattatt
     1201 tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg aacgatatgt
     1261 gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc taatatagta
     1321 agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca cattttttga
     1381 gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag actcatatct
     1441 agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa ctttcttcaa
     1501 ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct tcaacttcct
     1561 tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt gtgattgttg
     1621 ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt tttccttctt
     1681 gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac gtccgtgtca
     1741 ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg atcgttacgt
     1801 tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga gcaaatcgat
     1861 caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc tgattgaatt
     1921 ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc ttctctacgc
     1981 cgttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt gagacacgtg
     2041 catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc gtatactcag
     2101 aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg cgatcgtcaa
     2161 ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt atgggacttc
     2221 gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca actttttgca
     2281 attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt ccttctcgaa
     2341 atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa gaagtttgtg
     2401 gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca aatcgaaaat
     2461 ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata tccgaaaata
     2521 gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca tttatttttc
     2581 cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc ttagccaact
     2641 caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc cttaacttta
     2701 tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt tatgtgtcaa
     2761 aaactattaa gtttctgacc cccgatagat ttttcaagtg tctctatttt gcacagtcta
     2821 gt
//
LOCUS       DACDEG010000170         2802 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.176, whole genome shotgun sequence.
ACCESSION   DACDEG010000170 DACDEG010000000
VERSION     DACDEG010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.176"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(66..428)
                     /locus_tag="IV142_002728"
     CDS             complement(66..428)
                     /locus_tag="IV142_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486303.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(502..882)
                     /locus_tag="IV142_002729"
     CDS             complement(502..882)
                     /locus_tag="IV142_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP8486304.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            964..2139
                     /locus_tag="IV142_002730"
     CDS             964..2139
                     /locus_tag="IV142_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8486305.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2216..2800)
                     /locus_tag="IV142_002731"
     CDS             complement(2216..2800)
                     /locus_tag="IV142_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8486306.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          867 a          547 c          477 g          911 t
ORIGIN      
        1 ggaccaaaca ataaaaaaag aagtttgaaa aaaattcaaa cttctttgtt cgcttaatct
       61 gtattttaga agaaaggcac tccctctact tcgtaagttc caagaacaga gcgcatttga
      121 cctggttctt cccaaatata tccttcgcct aattcttctc tttgattctt ttgttcagtt
      181 tcaaagacaa acaacggaac attttcctct ttaatatgtg tatttgtcaa ttccatcaaa
      241 ctaatcttgc taacatcgat atgaactatt tcttttaaga aattaagtat attcgctaaa
      301 ccagtctttc cttcagaaag ttccgctaac gcaaattcca acttgtcatc aattgaatgg
      361 accaaaaatt ttttagtacc atcctccaaa tgcaacataa ttgtaccagc aactcttttg
      421 cttgccaaaa tttccacctc gatcaacttg ttctatggct tacataaaaa ttctagcaca
      481 ttttcagaaa aaatacactt tttatttctt attttctttc cgttctagtt cagataatgc
      541 ttcttctatc caaataggta actgctccgc aatagtggta aatccgatct tacgattttt
      601 attcgtgaaa tacctttttc tgcgatatgg aacagctgga tttgtttttt ctaacgttct
      661 taaagataaa ctgatttttt tggaatattc atcaatgtcg atgatctgaa ctgttactgg
      721 ctgtccgact tttaacaatg aatggatatt tttcgtataa ccggattgta cttctgaaac
      781 atgaataagt ccttgcgtct cgttatcaag agaaacgaat gcgccataag gctgtatccc
      841 tgtcactttt ccttcaatta tttgtccaat tttataagtc atctacttcc ctctcttcgc
      901 atttatcatt atactgtaaa tagctcaaaa aaagaagcga cgaaaatgac tggaggtctt
      961 aagatgatcg aatttgataa acagatttca agaaaagata cagaaagtgt aaaatgggac
     1021 gcaattgctg agacatatca aatggatgac ttacttcctt tgtgggtagc agatatggac
     1081 ttcttgtctc ccggtggggt agctaaagcg tttagtgaat acatcaaaca tggaatcttt
     1141 ggttatgcca ctctgtctga taaactatat caggctatca ttcattggga acgccaacac
     1201 catgctgtgg agttgacgaa agaaaatatc gtttttacaa gtggtgtgtt aaccagttta
     1261 gctgcagcag tacaaacttt tacaaaacca ggcgattcta ttttgattca tgacccagtt
     1321 tatcctccat tttcctctat tatagaaaca aatcaacgtc acatcgttcg aagctctttg
     1381 ctagaaaaaa ataatcattt catcatggac ttgtctgaca tggagaaaaa aatcgaagaa
     1441 aacaatgtaa aagcaatgat tttatgcaat cctcacaatc ctggagggcg tgtttggagc
     1501 aaagaagaat tgaaacagtt aagcgaattg tgtttaaaac atcaagtgat tctgtttagt
     1561 gacgagatcc atcaagattt aactttattc gatcatacat ttacttccat gctaacgatt
     1621 gatcctaatt tagatgatct actagttgct tttacttccg caaccaagac tttcaatcta
     1681 gctgcaatca aaaattcaat ggtatttatc aaaaatccag agatgaaagc tgattttgaa
     1741 aaacatttag tgatgaacca acagcacgaa atcaacactt ttggactgat tggaacccaa
     1801 gcggcttatg agacgggtgg agaatggtta agccaactga ttccttatct agaaaaaaat
     1861 gttcaaacag ttaatcagtt ttttgaacag tatcttccga aagtgagaat aatgaaccct
     1921 gaaggaactt acttaatgtg gttagatttt accgcctata tttcagacga ccaagaattg
     1981 gagaacactc ttgttcggaa aggaaaagtc gtattaaatc ctggcatcac gtttggtcct
     2041 tccggtcatg ggcatatgcg cttgaatatt gcttgtccag aagaaacttt actggaagga
     2101 cttaatcgaa tcaagtatgc tttagagaca atcgattaaa tcatttattc aaaataaaag
     2161 gacgagacct tactaggttt cgtccttttt cataattact ccgtaccata ccaaactatt
     2221 ctgaaatttc aattgtttca atcactacat catgtacagg tcggtcttgt ggaccacgtt
     2281 gtacatttgc gatttcatct actgtgtcca tcccttctag tacatgtcca aagactgtat
     2341 gtcggaaatc tagccaagga gttccgccac ctttatacgc ttcaatgatt tctgatggga
     2401 atccagcacc ttctagttgt ccaagcatat tagctggcac atttgtgtta tttacaataa
     2461 agaattggct tccgtttgtg tttggacctg aatttgccat agataaagct ccgcgaaggt
     2521 tgaacagttc acgactgaat tcatcttcaa aactttcgcc ataaatactt tcgccaccca
     2581 ttcctgttcc agttggatcg ccgccttgga tcatgaaatc aggaataacg cggtgaaaaa
     2641 tcacgccatc ataataacct tttttggaaa gttcaatgaa attttgaacc gtcttcggtg
     2701 ctagttcagg gaataattga acagtaatgt caccacggtt tgttttgata acagcttttg
     2761 gtcctttttc actttttaaa tctagttgtg ggaatgccat at
//
LOCUS       DACDEG010000171         2800 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.177, whole genome shotgun sequence.
ACCESSION   DACDEG010000171 DACDEG010000000
VERSION     DACDEG010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.177"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            86..1093
                     /locus_tag="IV142_002732"
     CDS             86..1093
                     /locus_tag="IV142_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8486307.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1105..2109
                     /gene="neuB"
                     /locus_tag="IV142_002733"
     CDS             1105..2109
                     /gene="neuB"
                     /locus_tag="IV142_002733"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAP8486308.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2106..>2800
                     /gene="neuC"
                     /locus_tag="IV142_002734"
     CDS             2106..>2800
                     /gene="neuC"
                     /locus_tag="IV142_002734"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP8486309.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQ"
BASE COUNT         1041 a          353 c          544 g          862 t
ORIGIN      
        1 attattgata attagtttag ttatatattt tttaaagatt aagaatcgaa ttatagatga
       61 ctataattca taggaggaga tataagtgtt taaaggaaaa acaatattag taactggcgg
      121 aactggttcc attggaagtg aaattgttcg tcagctttta aaatatgaac caaaagctat
      181 cagaatttat agtagaggtg aagaaaaaca attttatatg caacaagagc tctcaaatca
      241 cactaatttg agatttttaa ttggtgatat cagagactct caaaggattg attatgcatg
      301 tcgtgatgtg gatattattt ttcatgctgc cgcaatgaaa cacgttccag caagtgaata
      361 taatcctatg gaagctgtaa aaacaaatgt gatgggaact caaaatgtaa tagatgcagc
      421 gatcaaaaat aatgtaaaac attttgtagg tatcagtaca gataaagtag tgagtcctac
      481 taacaccatg ggtgcgacaa agttattgag tgaaaaatta ataatagctg ccaatcagta
      541 taaaggaaaa acagaaacaa tattttcttg tgtaaggttt ggaaatgtca tggggtcaag
      601 tggatctgtt attcctcttt ttagtaaaca aataaaaaac aataaagata ttactttgac
      661 agatgttgat atgacacgat ttatgatgtc aataccggat gctgcaagat tagttctgtc
      721 tgctgctatg aattcaaaat ttggagaaac atatgtctta aaaatgccgg caatcaatat
      781 tttaacttta gctcaatcat taataaaaat atataatgat acacatgatg aaaaatatag
      841 aggtgaaata aaagttgtag gtatcaggcc aggagaaaaa ttatatgaag agctaatgac
      901 tacagacgaa attgaacgct catatgaaaa tgataaaatg tatatcattc caccacacta
      961 tcatgctact gacgaactgt acaaagatta tgaaaaaatt aaaatcactg catactcttc
     1021 agatgcaaca gaattaatga gtggacagga atttattaga atcgcaaaag agaataatgt
     1081 cgtgacttgg taaggagttt tatgatgaaa tcagaagtat atgtaattgc agaaattggt
     1141 gtaaaccata atggaagtct agatttagct ttagaaagta ttgataaagc taaagaatgt
     1201 ggagctaacg cggttaaatt tcaaacattt aaaacgaata agctagtttc caaaaaggct
     1261 ccaatggcaa aatatcagaa ggataatctt aaagaggaca gttctcaatt tgaaatgcta
     1321 aaaaaattag aattatctca atctgatttt attagaataa aagattattg tatacaaaat
     1381 aaaattgatt ttttgtctac tccttttgat gaagaaagtg cagactttct aaaacaaatt
     1441 ggtgtacaag gatttaaaat tggctcagga gatttaacaa atttaccttt tcttagaaaa
     1501 atagatatgt atgggttgcc agttttatta tctacaggta tggctgatat tcatgaggtc
     1561 agtgaggctg tagattgttt catagatagt ccagttacta ttttacattg tacttcaaat
     1621 tatccggcta gttctgaaga tattaattta ttggcgatta acacattaaa agaaatgttc
     1681 gggatacccg ttgggtactc cgatcattca ttgggatatg atgttggtat ttgtgctgtt
     1741 gcgatgggtg ctaaggtaat tgagaaacat tttaccttgg ataaggaact acctggtcca
     1801 gatcataaag cttctttaga tcctcaggaa tttgcagatt ttgtaaagca tattagaaac
     1861 actgaaatat ttttaggtga tggtgttaag cgagcaatgc cttctgaact tagtacaaaa
     1921 gaagtatcaa gaaaaagcct agtggtcact gagaatttaa atgtgggaga tattcttaca
     1981 gagaaatctc ttacaataaa acgtcctgga aatggaatag ctcctaagtt tttatatcaa
     2041 tatattggca aaaaagttaa aagaccaata agtgcagata gtgttctgct tgaggaagat
     2101 gttgaatgaa aaaaatactt atagtaagtg gaacacgagc tgattatgga atatataaac
     2161 ctgttgctga gaatttacta caagataatt gggatgtatc atttgttgtt tctggaatgc
     2221 atttatccca taaatatgga tttactaaag aattgatctt agctgacgga tttagaatta
     2281 gtggagaagt tcaaagtttg tttttagaga acaataaggg aaacatggct cggtctgtgg
     2341 cattagagat aaatggtttc acaaatatat ttgaacaaac tagccctgat tttgtattat
     2401 tactagggga tagaggagaa atgttggcag ctgcgatagc ttgtttgtat cttggaatca
     2461 aaacagcaca tattcacgga ggcgaagtgt ctggtactgt agatgaatca ataagacatg
     2521 ccataacgaa aatagcatct gttcattttg ctgctacgga aaaaagcaaa gagcgtttaa
     2581 taaaaatggg ggaagattct tggagagttt ttcccgtcgg agctcctaga atagatacta
     2641 ttttaaataa tagattacct agttttagag atataaaaga aaaatatcat ctaaaatttg
     2701 aaaagaaaaa atacgttttg caagtatttc atccagttgt aaccgaacta gaaaatatag
     2761 aaaaacaaat tttagctttg atcaattcaa tggaacaaca
//
LOCUS       DACDEG010000172         2698 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.179, whole genome shotgun sequence.
ACCESSION   DACDEG010000172 DACDEG010000000
VERSION     DACDEG010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.179"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            172..516
                     /locus_tag="IV142_002735"
     CDS             172..516
                     /locus_tag="IV142_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP8486310.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            517..936
                     /locus_tag="IV142_002736"
     CDS             517..936
                     /locus_tag="IV142_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP8486311.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1028..1378)
                     /locus_tag="IV142_002737"
     CDS             complement(1028..1378)
                     /locus_tag="IV142_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486312.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1344..2675)
                     /locus_tag="IV142_002738"
     CDS             complement(1344..2675)
                     /locus_tag="IV142_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP8486313.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
BASE COUNT          999 a          490 c          522 g          687 t
ORIGIN      
        1 ataaaaaaag cgttagcaac tatgcttaaa aagcatatcg actgaataaa ctaagcattg
       61 gacaggctga aagactacat ttaaaatgaa atgcgaagaa acaatagaaa ataaagttgc
      121 cttagaacca tagttgaatc acctaacagt cgtgaaggga gaataagaga aatggcagaa
      181 aaacaaaccg caggacgaga tcaattagga gattttgcac caaaatttgc agaattgaat
      241 gatgatgttt tatttggaca agtatggtcc agagaagaat tatctgctca tcagcgtagt
      301 atcatcacca tatctgcatt aatttcagga ggaaattttg aacaattgcc agctcatctg
      361 aagattggca agcaaaatgg cattactagt gaagaaatag cagaaatcat tacccattta
      421 gctttttatg taggatggcc aaaagcttgg tcagctttta gtttagcaaa agaaatctat
      481 ttagaagaaa atagcgaaga taagaaagag gcgtaaatga cgacaaaaca cgaagaaata
      541 cacaatggcg tgatttttcc agcaggtgaa aaaaacgaag cttacgctca agtatttacc
      601 gggcaaagct atctgaatat gttagtgaat gatcccgaag tttctgttgg tgtaggtaat
      661 gtaacttttg aaccaggttg tcgcaatcac tggcatgtcc ataaagacgg ctatcaaatc
      721 ttacttgtga ccggaggaga aggctggtat caggaagaag gaaagccagc aagacgatta
      781 atggctggag atgtcgtcgt aacgcacaaa gggattaaac attggcatgg tgcgacaaaa
      841 gacagttggt ttgaacattt ggcgatcact gctggtaatt cagagttttt agaacctgta
      901 acagatgaag tgtatgaagg attggaaaat caataatgtc aagcttgaac agctcctgaa
      961 agtagatttt aatcgaactt tcaggagctg cttttttgat tcagtctttt ataaatagaa
     1021 aatagggtta ataataggaa tgagaagcac gtcgaaagat cttaatattg ataatcggct
     1081 gggcatacat gatttccacg tcatcaatca aactacctaa cagatgataa tcttcccgat
     1141 catgtcgggg attacctaat agttcttgat aatagatttc catactaggg tcccgaggat
     1201 tgttcagcaa atcagcaagt acatctaaat cttctggctg agtttctgcc gtaccctgta
     1261 tctctatgaa aagcccttcc tcagcaaatt ctactccaat acgtatttca gttatttcat
     1321 gggaatataa ataagtaaga agctcatcga tgattctttt tgataattct ttttccaagt
     1381 gtcttttttt cctttctgac agcggtaaag ttatctagta tcggtacaag gatacctgca
     1441 acgaaaccag ttgaaaaacc actgttatat agatttagtc ctccgtgcaa agtgctgacg
     1501 ttatggacaa gagcaatatg tagtacacca gctattacac cgtaaaaact gccgtaaaaa
     1561 ccgcttatgg gcgcgagacc tgtcccaaac acagctgcaa caagcatgcc agtactggtg
     1621 atctcatgaa aagttccaaa caaagaagca ataaatattc cgactagtat cggaaaactg
     1681 ttttttaaat gacaaccaaa agcactgaaa cccaccgcag ataaaatagc accgatgaca
     1741 ggtccattta actgaccgcc aaccagcaaa acataagaag acagcatcag accaactaag
     1801 cctatgttga tcaaagtagc accaatacca gaattagcaa tgaaatcagt tgtcaatttt
     1861 ccagatgaat cgaaaatttc tcgaatcccc aaaagccgaa aggaattata atagaaacca
     1921 attccgactg tcagcgaaaa aaagccaaaa ataatccaaa tgagaggaaa gtgatattca
     1981 ttgaaaacca atgtattttc gacgattgaa tagctgaata acctcaaaac agcagtaaaa
     2041 gtcatcgcaa tcaacccaga ggtgaatcca atattataaa gtgtgaatcc acgatggaaa
     2101 actaaagttt gagcagctaa gggcggaaga agtagaccaa ttgctatgcc agctaaattc
     2161 cccaataaaa tacctatcag taaaggaaaa cgcataccaa aagtgatata actaacgacc
     2221 ggtgataatg cgctaccaaa cagtccgatc atgatgtact gagaaaaagg tttattgaca
     2281 aatacagcgt atagatacac acctaaaatg attgagatag aattataaag gtttttgcca
     2341 aaaaaagaga aaccggtaac tgttagaatc gaagcaaata agggaccgtt taaaatcacc
     2401 ttgtgccgat aagctaaaag cacagaaagt aaggtcaaaa tccctacatt tatgaatgcg
     2461 cttccgaaac cgcctaaagc aaaataatct gtcaaaagat tgcttggcga ggtaagaata
     2521 gttagcaatc cactccaatt atcagtagga gtattagaaa gcaagccaat aaaaagaaaa
     2581 aaaacacctg taaaaccaag ataaaaatac tttcttttct gtgaaacgat tcgtttttga
     2641 ggatgaatat aaataacgtc agtttcgttt tccattacac accttctttt tcattttc
//
LOCUS       DACDEG010000173         2653 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.180, whole genome shotgun sequence.
ACCESSION   DACDEG010000173 DACDEG010000000
VERSION     DACDEG010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.180"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(289..735)
                     /locus_tag="IV142_002739"
     CDS             complement(289..735)
                     /locus_tag="IV142_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP8486314.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            1330..1680
                     /locus_tag="IV142_002740"
     CDS             1330..1680
                     /locus_tag="IV142_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486315.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            1885..>2653
                     /locus_tag="IV142_002741"
     CDS             1885..>2653
                     /locus_tag="IV142_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HAP8486316.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNAL"
BASE COUNT          898 a          532 c          414 g          809 t
ORIGIN      
        1 actcaccagt aagtttaatc tatttagcaa aagaagctat gtctacactc tttaaaatca
       61 atatgttttc tcatttttaa agtttcaaaa aaactctata actaagaaaa atcacaaaaa
      121 agagtacatt ttctagtgaa actgcaccct tttttgcttt gtttacgtac aaaaaatctc
      181 ttgagtactt aacttctaca gtataatcaa caaccccttt atagattact gatgttatgc
      241 tgaaaaaaat tttttcacca acttgtaatc taagccactt ttcttatttt acttctcaaa
      301 ccaatcataa aattcatctg gtgttttctc ccgatagagt ttgattaacg ctggtcgcat
      361 cgattgaaat atttcgacta attcttcccc accaaatcga gtggaaaaat gcttatcagt
      421 aaatatcatt gataaagaag aaagaaacag ctccgtatct tcatctgtac caagaatatt
      481 gatcaacttt ccaatcaaat aggaatcatt aaatgtcagg tcttgtttca catcaaaggt
      541 ttttattgat tctttcacca ttttcgcaat gcgatctttc tctccatact tgatctcacc
      601 actcatctcg atatagtcaa tgatcccaga tgaaagaatc gtgacagctt cttcttttgc
      661 ttttgatgat agtttgttta gcgttttgga ttcttcaagt acctctttcg taatctgtac
      721 gacatagtcc ttcatgattt cactcgaata ttctacaaaa cattcttgga taaattcctc
      781 caaaggatag cctagctcat tctcccaagc aggatttttg aactcgcccg tataaaattc
      841 ttgctttatt ttctcaatga tttcaggata atcgtccact atttttttct gaccaatgag
      901 aaacaaatac tctcggatat attcttccac atgcccaaaa taaaaccagc cgacaggttt
      961 tccgctgata ctagataatt ggttgatcac actcttagga gccaacgcat agccttgaac
     1021 gtaggaactg atcgtcggct tttttaaacc taatcgattc cctaattctg taaaagagag
     1081 agacattccg tcttttattt ctttgattcg ttggcccacc ttcactttat taggttgtac
     1141 taaaccaaac atattcaaat tcctccttat catttttttc attataacac tcgtataaaa
     1201 aatcgtatat atttttttag aaaatctctt gactacgtat aagttctcgt atataataca
     1261 agtcgtagat ggccatctat attttttaat caaatcgtat aaactttcgt ataaaaaagg
     1321 agaattgtga tgccaataaa aattaccgat ctcagtttac caaaagaaac actcgaaaaa
     1381 gaataccaag tcgtcaatgt cactcgctgg caaaaggagg gcgaaattct aggttggagc
     1441 tacgaatgta tcctaccaaa acttcgctac gaaaaaatca gtgtcaaagt caaatctgaa
     1501 catcctgtga tcacgccgga agaacttacc caacaaggtt tagccataat tacatttacc
     1561 aatttggtga ttgtaccttg gggacgctcg aatggacaat ttgtcagtta tgggttatcg
     1621 gcaaccgctg atacggcaac tttgattccc agaaaacaag gtaccactac acaaaactaa
     1681 ctatactatg gagtgttggg tggttaggac gaaacggcgg ggcagcgccc acagagccgt
     1741 ttctgtccgt taagaccgcc caacacccca taggctagaa gggctgctac gcaggccttc
     1801 ccccttaact tgatttatag atcaacatca ggagaaaatg actggttctt tgacaatcga
     1861 atacgcaaca aggagtgatg atttttgcaa gcttataatc agaaaatcat tgaaactccc
     1921 acgtatattg aaatttatga ttatgaagca atcattactc ccaaacgtaa aacgatcgaa
     1981 aacgaagtaa atctggaacg aaaacaaaaa ttagcttgga tttatgaaaa tgatattact
     2041 cttgaaaaaa gaaaaaacta tgatgaatta tccgctcaag gacaatatga cagtttaaaa
     2101 cgcaaacaga aacattatca aagtatgcga tttgagattg cccgccttgt agacacgaat
     2161 tttgacaatc aaacgaaatt tttaactctc actttcaaag aaaacattca agacattgag
     2221 tatgcaaata atgaatttaa aaaatttatc aaacgactga attatgacct ctataaaact
     2281 aaaaaagcaa atttaaagta tctcgccacg tgggaaaagc agcaacgcgg tgcaatccac
     2341 taccatatta tcttgtttga tttccccttt atctcattta accggttaac tacgatttgg
     2401 ggtcagggtt tcgttaagat caacaagatc gatgtagaca gtatcgaaaa tcgcgggcgc
     2461 tatatcagca aatatttcga taaagagctg gaattgaaag agcataagaa aaaagctttt
     2521 ttcaagtccc ggaatctcaa actaccaact gtcaccaaaa agatggtaaa gaaaccctat
     2581 gattccacga atcaagatat actatataca agcgaatata tagttagacg tccatttttt
     2641 tacaatgctt tag
//
LOCUS       DACDEG010000174         2642 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.181, whole genome shotgun sequence.
ACCESSION   DACDEG010000174 DACDEG010000000
VERSION     DACDEG010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.181"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(34..279)
                     /locus_tag="IV142_002742"
     CDS             complement(34..279)
                     /locus_tag="IV142_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8486317.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(273..674)
                     /locus_tag="IV142_002743"
     CDS             complement(273..674)
                     /locus_tag="IV142_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8486318.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(846..2639)
                     /gene="uidA"
                     /locus_tag="IV142_002744"
     CDS             complement(846..2639)
                     /gene="uidA"
                     /locus_tag="IV142_002744"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP8486319.1"
                     /translation="MTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESYPISVP
                     SSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNGKLINQ
                     HKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKKENLVN
                     FDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILIAIYDE
                     EAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGIRSIQV
                     SNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTSHYPYS
                     EEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKMAIKEL
                     IERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYLKSTPD
                     ICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTEYGADT
                     VAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILRVQGNK
                     KGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
BASE COUNT          792 a          510 c          348 g          992 t
ORIGIN      
        1 ttaaacagaa taagtggaaa ggggactttt tagttagtta attttaaatt ttaagatcgc
       61 ttctatcaat ttaatttcta gctgtcttag catatactca tctacatagg taataaattg
      121 ccccgcctca ttaaatccat ttgtcaaaca taacttagct atataactat gataatgttt
      181 gagtatcatc ttgattgaaa ttggatcttc ttctagagca gattggataa tggatagagg
      241 aactaatgga tactgccgtt ttttggggtt gtctaccata agtcataccc catttttaat
      301 tgttcaactg ccttctttcg ttgataccag ataccacttg tactcatatt gaggatttta
      361 ccgatttcag tatcattaaa acctgcaaaa taataaagta ggataacctt tcttcttatt
      421 tccgataaat gatcaatggc tgccgataac ttttgatctg aaattaatat ttccattcca
      481 aataagaaat agacatcttg ttcatcaaaa tgagtttggg agtacttacc ttcatttgaa
      541 ttcgtcaatg gttcctcatt atctgttatg ttaatgtttc gttttcgaat agacttagtt
      601 tcgtttcgta atactttttt acagtaggca tcaaattgat gttgtataga tttctcattg
      661 taattttgtt gcatgataaa tatgctcctt ttttcccctt tccactaata caacaatcag
      721 aaagcgcctt tttttcgaaa aaactagaaa aaaatatcct tctttaaaca gaaaatcttt
      781 ttataaaaaa aaggagttgg caattaagca gccactcctt ttttatattc cttataaaag
      841 gtaaattatt ttttataact gaaatttggt attgatgacc atcgtttctt tagatagtga
      901 gcaatcattt taggctttct atctctagta aagattcctt tcttatttcc ttgcactctt
      961 aaaatccctt gacttgtttc aaaatctgca aaattccaca cttgttcacc aacaaaattg
     1021 tcacatttat ctattacttc gtgatttgct tcgtaataac ccacttgata ctcttctgta
     1081 aacattgttg gtactgtatc atgaagtcca gctactgtat ctgctccata ttctgtaaac
     1141 attattggcg tatcgggaca tctttccatc catccatcta gctctttttt caacagatat
     1201 tttgcatgtt ttaggtctcc acctgaaaca taccaaccat aatagcgatt taaacataaa
     1261 acatcgacaa tatctcctac tttacaaata tctggtgttg actttaagta agtaacgatt
     1321 gttacaggtc tcttctgagg gtcaagttct ttagtcaatt caactaaagg ttcaaaatat
     1381 tctttagcgc cttcagaatc cgaatctggt tcatttgcaa tagaccacat tacgacactc
     1441 ggatggttct tgtcgcgttc tatcaattcc ttaatcgcca ttttgtgagc attttctgtc
     1501 cgtatttctt tccaagtatc atgtttttct acgttgtcat ctaataaagt agccatgaaa
     1561 ttcaaatgta atcctactgc aggtacttca tcaataacta caataccttc tcgatctgct
     1621 aagcgcataa tttcttctga ataaggataa tgtgaagtcc taaatgaatt agcaccgatc
     1681 catttcatta aattaaaatc tttgatattt actgcttcat tcaaaccacg accattaata
     1741 tacgtgtcct catgcttccc aaatccctta aagtaaaaag gtttattatt aatcaaaaac
     1801 ttcccattcg aaacctgaat actacgaatg ccaaattctt ctgtgtacgt atcaatcact
     1861 ttattttcgt taattaaact aactttcata ctatataagt aagcatttag aggctcccaa
     1921 agtgttgggt tcttaagttg aatcactcct tctttcccct tagcttctgc tataagttga
     1981 gcttcttcgt catagattgc tattagaatt tctccggtcc attctgtatc gatattaaat
     2041 tctacatttg catctgaatc aattattttt gtattaatag tgacatcctt tatatagtct
     2101 ttcggagttg tatacaattt aacgggtcta ttaatgcctg cataattgaa aaagtcaaaa
     2161 ttaaccaaat tttctttttt tatgtctcca tcttcctgta tcgtttcttt caaaatccct
     2221 acaggtaaag tgctatcgtc aataacattg tttacagcca ctgtaattct gttacttcca
     2281 tcttttataa actgattaat ttctgattca aatggcgtaa accctccttt gtgttgatta
     2341 atcaattttc cattcaaaaa tacttttgct tcatgtgttg cagatccaaa acgaagaact
     2401 aatctttctt ctaataattt tttaggaaga atcacttctt tttcatacca cacccatcca
     2461 acatgatttc taatgttctc tgtttcaaat aaatcattat aagaagaagg aactgaaatt
     2521 ggataactct ctttttcaac cattggttct aaagcaagtt cttcttgtaa gtcatcattt
     2581 gtatctaact taaatctcca aagaccgctt aaatcaatta cttgcctcgt ctctgtcata
     2641 at
//
LOCUS       DACDEG010000175         2631 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.182, whole genome shotgun sequence.
ACCESSION   DACDEG010000175 DACDEG010000000
VERSION     DACDEG010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.182"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(61..2424)
                     /locus_tag="IV142_002745"
     CDS             complement(61..2424)
                     /locus_tag="IV142_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HAP8486320.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT          700 a          553 c          483 g          895 t
ORIGIN      
        1 cttaatttga caaataatac aatgtaaaaa attctgtttt gcgggatcga ttttatcatt
       61 ttattggtca agatttttcc atttccaatc caatacttct ggcagatcaa taccattttc
      121 acgaatatag ttttgatgtt cttgtaattt ctgatccatt ttttccgcaa aatcttctgc
      181 ttgacttcct ttgattttta aagctgcgtc ttttgccaaa tgataacgat caagttcatt
      241 tactacacgc atgtcaaatg gtgtcgtgat atctccattt tctttgtagc catgaatcat
      301 taaatgatgg ttatgacgat caaagaaaat cgtgcggacc aatgtttcat aaccgtggaa
      361 agcaaataag atcggtttgt cttttgtaaa atattgatca aattcttcat cgctcaaccc
      421 acgcggatct tgacttggat gacgtaattt caataaatct acgacattga tgaaccgaat
      481 ttttagtttt gggaattctt ccagcagcaa agttacagct gctaatgctt ctaagttaga
      541 ttccgttcct gcagcagcaa tgaccaattc aggttcctcc tcttttgctg tcgaagccca
      601 atcaatgatt ttcaagcctt cattcactaa gacggctgct tcttctgctg agtaaaattg
      661 agcacgcgga tgtttggaag agatgatcaa attgattttt tctgacgagc ggaatgcttt
      721 atccatgact gcgagcaatg tatttgcatc cgcaggcaga tattcacgga tgaactccgc
      781 cttcttttct gccaaatgtg tcaatattcc tggatcttgg tggctatatc cattgtgatc
      841 ttgctggaag actgttgagc ttgcaatgag attcagcgaa ggataatctt ttcgccaaga
      901 aaggtcatgt gatttccgta tccatttgaa gtgctgagtc aacatagagt caaccactcg
      961 caagaatgat tcgtaacttg caaaaaaacc atgccgtcca gtcagaacgt acccttctaa
     1021 gaacccttca tcttggtgtt ccgataattg actgtcgatg acgcgaccag atggcgaaag
     1081 ccattcatca ttttctggtt cgattttttc catccattgg cgatttgtcg ttttgaaaac
     1141 ttgattcaat cggtttgatt ttgtttcatc tggtccaaat atacggaaat tttctggatt
     1201 tttctcaatg atctcgcgaa cgaatttacc aagttcggtc atatcttctg ctttaacgga
     1261 gcctggtttt tcaaattgca aagtatattt tttccaatct ggcataataa gcggtcttgg
     1321 atcgattcca ccattggtga taggattttt tgccattctt tggtcaccag taggcgcaat
     1381 ttctctgatt tcttctaaaa tacgtccttg tgcatcaaat aatttttctg gttcatagga
     1441 tttcagccaa cggagtaaag catctgcatg atccatatgt tcttgatcta caggaatcgg
     1501 tacttggtgt gctcggaagg ttccttcaat tggttccccg tcccattctt ttggaccggt
     1561 ccatcctttt ggtgtgcgta cgatcaatac tggccatttt ggcatcacga catctgttgc
     1621 tgtcttcagg cgagcttctt tttggatgga tttgattttc tcgattgctt gatccatttt
     1681 ttcagccata acaggattta atttttctgg atcatttcct tcaataaaga aaggctccca
     1741 gccaagtccg ttaaagtaat ttgctaattc tccatctgat ttacgtgcca agatcgttgg
     1801 gttggctatt tttgcaccat ttaaatgaag gatcggtaaa acagctccat ctatcactgg
     1861 attgataaag acatttgaga accagcttcc agctaaagga ccggtttctg cttcaccatc
     1921 accaatcacg acagcagaga ttacttcagg attgtctaat acagctccga ctgcatgtga
     1981 aagagagtat ccaagctctc cgccttcatg gatagaacct ggtgtttctg gtgctgcatg
     2041 agaagcaatg cctccaggaa atgaaaatcg cttgaataat ttttgcattc ctgtctcatc
     2101 ttccgttact tctggataga tctctgtgta acttccatct aaataagcat tcgataccat
     2161 gacttgtccg ccgtgtcctg gcccttcgat ataaaacatg ttcaaatcat atttattgat
     2221 gactcggttc aaatggacat aaataaaatt ttggccagga atcgttcccc aatgaccgat
     2281 tggatgtttt ttgacatctt ctggtttcag tgtacgtctc agtaaggggt tatctttaag
     2341 ataaagttgc ccaacagaaa gataattagc agcacgccac caagccgtca ttttatcaaa
     2401 gtattcttta ctagaataat ccatacataa cactcctttt cattattact cttattctag
     2461 cgcagatact cccaattgaa acctaataag cacttctgtt catttgtttg gattttatat
     2521 ctagccaaat gaacaggtat aaaaaaccac gaaatttttc ctttcgctga cgttttttac
     2581 attgatccaa aaaacttgtc gttatcacaa tggctgttcc gcagttcatt t
//
LOCUS       DACDEG010000176         2495 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.184, whole genome shotgun sequence.
ACCESSION   DACDEG010000176 DACDEG010000000
VERSION     DACDEG010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.184"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            121..981
                     /locus_tag="IV142_002746"
     CDS             121..981
                     /locus_tag="IV142_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HAP8486321.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQEIKNYQIKNKLICHCSGAISS
                     DIFSNIQDYGAYGYSVHPMFSISDKYNSYKKLKKSFITIEGDEKYAKFLCDFFKKLGN
                     STVIVSKENKSLYHAASVVSSNLVLGLINTSVTYLIQCGFTEKMAIEALYPLIEFNIR
                     NIKEKGIIESLTGPVERCDISTIKGHCEVLSDEDRALYMLLSKNVLEIAMLKNINRDY
                     SELEKYLGES"
     gene            993..1820
                     /gene="panB"
                     /locus_tag="IV142_002747"
     CDS             993..1820
                     /gene="panB"
                     /locus_tag="IV142_002747"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAP8486322.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            1835..>2495
                     /locus_tag="IV142_002748"
     CDS             1835..>2495
                     /locus_tag="IV142_002748"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAP8486323.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DS"
BASE COUNT          950 a          274 c          506 g          765 t
ORIGIN      
        1 tatgggatat tatgataaat taactatgga tgatatttga aaaaaacata ctgtatattt
       61 gatttaattt ttatttttaa gctcatatcg taagaatggg acttgtagtc tcacttcttt
      121 atggaggtga taattatgaa cgttggtttt attggagccg gcaaggtagg ttgttcattc
      181 gggaagtatt tccaggaaca taaaatacaa gtaacaggat tttatagtaa aagcgaagac
      241 tcgtcattag cagcttcgaa ttttacatct agtaaacaat atttaaactt aagagaatta
      301 gtagatgaaa atgatactat tttcattacg actcctgatg gtcaaattca ggaggtatgg
      361 caagaaatta aaaattatca aatcaaaaac aaattaatct gccattgcag tggtgcaata
      421 tcttcagata tcttttcaaa tatacaggac tatggtgctt atggctattc tgtccatcct
      481 atgttttcaa tatcagataa atataattca tacaaaaaat taaaaaaatc ttttataaca
      541 attgaaggcg atgaaaaata tgcaaagttt ttatgtgatt tcttcaaaaa gttaggtaat
      601 agtactgtaa ttgtatctaa ggagaataaa tctctttatc atgccgcatc agtagtatct
      661 agtaaccttg tgctaggact tattaataca agcgttacat atctaattca atgtggattt
      721 actgaaaaaa tggcaattga agctttatat ccattaattg aatttaatat tagaaatata
      781 aaagaaaaag gaattataga aagtttgaca gggccagttg aacgttgtga tatttcaaca
      841 attaaaggtc actgtgaagt tttatcagat gaagacagag ctttatatat gctattatca
      901 aaaaatgtat tagagatagc tatgttaaaa aatattaata gagattatag tgaattagaa
      961 aagtatttag gagagagtta ggagagaaat agatgaaaaa tacagcagtt acatttaaag
     1021 aatctaaatt aagaaatgaa aaattaacaa tgcttacagc ttatgactat tcaacagcaa
     1081 aaataattga tgaagctggg ataaatggaa tattagtagg agattctctt ggtatggtat
     1141 gtctaggaca tgaagataca ctttcagtaa caatggaaga catgattcat catactagag
     1201 cagttacaag aggagctaag aacactttag ttgttgcaga tatgccattt atgtcttatc
     1261 aaacttcagt atatgattca gtagttaatg caggaagatt aataaaagaa ggacgagctc
     1321 aggttgttaa attagaagga gggatagaag tatgcgataa aatagaggct atagtaaagg
     1381 cttctatacc tgttatggcg catataggtt taacacctca atctgttaat gcatttggag
     1441 gatttaaggt acaaggaaag gacaaggaag ctgctaaaga attaataaga gctgctaaag
     1501 ctgtagaaaa agcaggagca ttcgctgtag tgttagaatg tgttccaact aagcttgcag
     1561 agttaatttc taaagagata tcaataccta caattggtat tggagcaggt gcagggtgtg
     1621 atggacaaat tcttgtatat caagatatgt tggggatgta tagtgatttt acccctaaat
     1681 ttgtgaagaa atatgctaat ttatcagaag agatgaataa agcatttaca aaatatatag
     1741 aggaagtaaa agatggagtt tttccaggtc cagaacatgg gtttaaaata agtgatgatg
     1801 ttttagaaaa attatattaa aagggtcaag aaatatggtt atatttaaaa cagtgaatga
     1861 tgttagaagt caagtaaaag agtggaaaaa acaaggctta aaggtaggat tagttcctac
     1921 tatgggatat ttacatgaag gtcatgaaag tttaattaga aaagcatcaa aagaaaatga
     1981 taaagttgtt gtaagtatct ttgtaaatcc aactcagttt gggaaaaatg aagatttagg
     2041 tagttatcca agagacttag aaagagatat agaagtatgc acaagaggta gagcaacagc
     2101 aatatttaat cctgaagttg aagagatgta ttgtgacaat gcaagtactt ttgttaatat
     2161 aactggatta acagaaggtt tatgtggagc tagtaggcca atacatttta gaggggtttg
     2221 cactgttgta agtaaattat ttaacataat accagcagat agagcttatt ttggagaaaa
     2281 ggatgcacaa cagttagctg taattaaaag aatggttagg gaccttaata ttgatataga
     2341 cgttgtaggg tgtccaatta taagggaaga ggatggacta gctaaaagtt ctagaaacac
     2401 atacttaagt ttagaggaaa gatcttcagc tactatttta aataaatctt taactttagc
     2461 taaggaagct ttaaataatg gagagagaga cagct
//
LOCUS       DACDEG010000177         2429 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.185, whole genome shotgun sequence.
ACCESSION   DACDEG010000177 DACDEG010000000
VERSION     DACDEG010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.185"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            106..300
                     /locus_tag="IV142_002749"
     CDS             106..300
                     /locus_tag="IV142_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486324.1"
                     /translation="MIFAAVAAVASIVLGGIFFFFGRRGAENGKMHVGYVLMGVGVIL
                     AAASIVVSLFGMFGQTPQGL"
     gene            369..2423
                     /locus_tag="IV142_002750"
     CDS             369..2423
                     /locus_tag="IV142_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486325.1"
                     /translation="MYFTISGNTKKDKELIKKATKKVGKRTAFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVSQVSEVEVNDEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDLVEA
                     NEEPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWERTKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCIE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMERHGQIQQELQAQIPKWKKEYEIQQQELKEQEEKQRQEEKEREQLELKKREQH
                     LAEQKVAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTAESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
BASE COUNT          965 a          373 c          523 g          568 t
ORIGIN      
        1 tttgaatcaa ttacaatgaa ttttttaaca caaccgaaag tagtagctgc ggttgatatt
       61 tttagagcat tacaaggtgc agcaactagt ttaaaaaaca atctgatgat ttttgcggct
      121 gtagctgccg ttgcctccat tgttcttggg gggattttct tcttctttgg ccgaagagga
      181 gctgaaaacg ggaagatgca cgtaggttat gtattgatgg gggtaggagt tatattagct
      241 gccgcctcta ttgtggtttc gttatttgga atgtttggac aaaccccaca gggactataa
      301 aaaataaata gaaggagtaa aaagtacgtg gctacttttt actccttttt tatgaaagga
      361 gaatactcat gtactttacg atttcaggca ataccaaaaa agataaagag ttaataaaaa
      421 aagcaacgaa gaaagtggga aagcgtactg cctttttacc gaaaacaatt aaatattctg
      481 tcattgaaac ggaagaacag ctacatgtgt taggacaagt gttagaacaa gagaatcaat
      541 cttctgttaa attgactctc acgttacatg ataaagaagg aaaaatagaa aaggctggca
      601 cagaatacgt ttttggccca ctggattatc aaatttcaga aggtttttat cctacattga
      661 tggatttgat ccgccttgat gtgtttaagg aaacggatga aaccatgagt gtggaagagt
      721 cttatcgaaa cgaaaaactg ctacaagttt tagaagaaga gttagagagc gaccaaagaa
      781 aagtgagtca agtgagtgaa gtcgaagtaa atgatgaagt accatgggaa cgaccaatct
      841 ttccaaagaa ggagtcaaaa gaaaaagaga cagtctatgt tgacgaacaa gctgatattc
      901 cgactcagaa atcgatattt gaggaagaga acgatgtaat gggtcaattg gaggtagatc
      961 cctttgaaga aacaatggag gatgaagaga cggccgaagt agaagttgat ctagttgaag
     1021 ctaatgaaga gccaacatgg actgaagggg attctcagga atatattgaa ccacttatca
     1081 gtgaagaagt agagaaaaac gaaacagaga tacaaaaggc aactgtattt gattatattt
     1141 tggatcaata tccgccagag catgaatgga tgaaagaaaa aaataaatta tttttaaatg
     1201 agctttattc gaaatatcaa ttaccccaaa cctatcaagc atttttagag agtaaagaag
     1261 aattaatctc acagggtaaa ggcacacttt tagaacagtt agagtctgtt caatcaacaa
     1321 attggcatga gcttgcacaa gaagatttat tagccacttt tgaagaacgt caacaagaaa
     1381 cagatcaaga aataaatgag tatgcgacgg aacagtcatc taattgggag agaacaaaag
     1441 aggaactaaa taaagaagaa caacgagtga ttgaagaaga agtacaaaaa attaaagagc
     1501 gatacgaaaa gaaacgcgac aatgcgtatt ccttgtgtat agagaaaatt aataacttta
     1561 ctaaaactaa tcaagaagct ttatcgaaag aaaaagaaaa cttgttaaaa gaacgtataa
     1621 aagaacgaaa agaagagttc taccgaggtt tacgtacaga taagctaaag attagcaaag
     1681 aattaaatga acaactgaat aaactctatc aaactacgac tgaagttctt atggaaagac
     1741 acgggcagat ccagcaagaa ttacaagcac aaattccaaa atggaaaaaa gagtatgaaa
     1801 ttcaacaaca agaattgaaa gaacaagaag aaaaacaacg acaagaagaa aaagagcgcg
     1861 aacaattaga actaaaaaaa cgtgagcagc atcttgcaga acaaaaagtg gcgatagaaa
     1921 aacaacggtt agagcaagaa aaggaaaaag aagcagctgc tcgtaaggaa cgtcaagaac
     1981 aaatggatac attgcgacaa gcacagttga attatctagc acaacctcaa ccagtagctg
     2041 ctgttcaaac gccagtgaac aaccaagcaa atttaactcc ttcaccacag cgttccttta
     2101 acggatggat ggtgggatgt attgcaagtt taagcttgct tctaggtgga ggaacagtat
     2161 ttgcttttaa tcatatcaat caatcttcac aggcagattc tgtagccaca acggcgtcaa
     2221 tcaaggaaga agcccaggaa caagcaagaa aagagtatga acaacaattg gaagaattat
     2281 tgaatcgtac caatcagtca acagcagagt caagtatgga aaatagcaaa gagagtgatc
     2341 ctagtaaggc taaagaagga tcaagcgatt caactgtaca ctcaaacact tccgaaagtt
     2401 cagaatcaaa tgaggtgaaa taatgcgta
//
LOCUS       DACDEG010000178         2418 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.186, whole genome shotgun sequence.
ACCESSION   DACDEG010000178 DACDEG010000000
VERSION     DACDEG010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.186"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(356..859)
                     /locus_tag="IV142_002751"
     CDS             complement(356..859)
                     /locus_tag="IV142_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP8486326.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1218..2243
                     /locus_tag="IV142_002752"
     CDS             1218..2243
                     /locus_tag="IV142_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8486327.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
BASE COUNT          774 a          373 c          454 g          817 t
ORIGIN      
        1 gggagcctaa ataaggagaa aaaccataga aaatataaag taattactta taaaacaagc
       61 tttttataga aaaataatat acctgagtag ggtataaatt aagacggaat tcctttctag
      121 attgagatat tgactaggaa gaatgttaag attttaagcc tttaagctaa aaaaaaaccc
      181 tttttgttaa ataaggggaa tatagcctgt cagtttcaaa ctgcaagtcg tcctgctgca
      241 ataatatagt agattagaga tgaaaataga tttttttaaa ccaatgactg aataaaaaga
      301 ccgtcataga tacacattgg aatgtgtcta ttacggtctt tttttacgaa cgattttatt
      361 gaagacggac agttcgggtc aaaaataaaa aagctacgat cagaaataag aataaaatgt
      421 atcccaaaat aatggctaga cggtctaact tatttgtcga tagatgtttg tttagaagtt
      481 gataggccca taaacctatc agtcctcctg ttgtattagt tatcaaatca gtgatgtccg
      541 ctacgccaat accgaatagg aattgaatca attctgccaa gaagcttaca aggaaaataa
      601 tccctaattt tttagaaaaa gaaagtctct tgaaattgac attcaaaagc accccaaaag
      661 gaaaaaagac aatcaagtta taagtggtct cacgtaatac cgtcgtattt tcaaaagaaa
      721 aagtagaaaa agggacgaga ttgacattgc tgtaagaata agtgagaaat tgtggaagat
      781 taaatgatag cttgaataaa atcaaccata atagaaagaa taaataaatc cctaataaaa
      841 attttgatac ataccgcata gacatctcct ccatttaaaa agtataccat cgaaaaccgc
      901 tgaaaaatca taaaaaagta tgaagtttaa ggaccaacgt agttatcgta cgaaaattag
      961 aagctttttt ttgttgggaa tccggtttct ttaacaaagc taagatagcg agttgcttgg
     1021 aaaagttgct atcttttttc tgtcaaaacg actcggaatg ttcgtttttt gttctaattt
     1081 ctccaagttg tttacttttt gatactaagt ttatgaaaaa tcaagaaata gttgttatac
     1141 taggtaatgc tgacaaacgg gattggcatt tcatcttggt tcagcttttt ttccaattac
     1201 taggaaggag ggatagattg ttcaagactt attttcgttt caataaaata caagataatc
     1261 cttttcgtat catgaatctg atgctactta tctttattgt attatggtta ggttacataa
     1321 atcatgattt tttgatttca agtttttcat cattagggat ctttactttt ttctattatc
     1381 aaaacatacc gatgaaatat ttgctgaaac gtatgtttct gatcggttgc ggactgttgg
     1441 ttgctttcgt tttggggatc ttatcgactt atgttttatg gcttgaacca tttgccgtag
     1501 ctttggttgc tttttccagc agattcgtgt tgcgattatt ccatatttct aaaccaggtg
     1561 gcttgttttt tgcgatgctt tctgccatgg gaacgagcat gcagctgcca atcgctcaat
     1621 taccgatcgt ctcactttat ttttttatgg gtgtcgtttt tgccctgatt gcggcagtga
     1681 ttacaaaatt actggattca cggcctgagc agacaattga aaaagcaact ttaaaagaaa
     1741 gatttcacga agagccttta gtgatcattg attctgtttt ctacagcgct gccttatttc
     1801 tttccgttta tgtcagtcac ggcttgaatc tgcataatcc ttattggttg accttgtcat
     1861 gtgcatcgat tttgctagca gaaaacttgg atgcgatgaa gcatcgacaa gtccaatatc
     1921 tgattggatc gatgggtggg ttatgtgtgt cggcattctt gtcatttgtg ccgtttacgc
     1981 agttgcagac catttttctg atcacttttc tatatggaat cgcacaattt ctagttgcta
     2041 gaaattatgc agttgctaat atttttctta atccgatggc gttgatgcta tctacactta
     2101 ttcgtggagc atacttgatt tctttgatcg aatatcgttt tttagggatc gtcataggaa
     2161 gtttcatcgg tttaggtgtt gcgtgggtaa tgacggttgg tttgcaacat tatttaacgg
     2221 ttgttcgtca aaaattagaa tagaaggaca aatatatgtg ccaaaagagg ttatgacaag
     2281 atttttgtca caacctctaa ttcaaggtgt tcaaaagcca gattctcagc attaagtcaa
     2341 attcacacaa aaatgtggaa aggaactttc aaaaaaattt tatatttatt ctatcaaaaa
     2401 attttgttta tgatggta
//
LOCUS       DACDEG010000179         2414 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.187, whole genome shotgun sequence.
ACCESSION   DACDEG010000179 DACDEG010000000
VERSION     DACDEG010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.187"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..323)
                     /locus_tag="IV142_002753"
     CDS             complement(<1..323)
                     /locus_tag="IV142_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486328.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINV"
     gene            complement(1037..1327)
                     /locus_tag="IV142_002754"
     CDS             complement(1037..1327)
                     /locus_tag="IV142_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486329.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1535..2227)
                     /locus_tag="IV142_002755"
     CDS             complement(1535..2227)
                     /locus_tag="IV142_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP8486330.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          701 a          450 c          342 g          921 t
ORIGIN      
        1 ttaacattta ttttctcaaa aacatacttg caaagtaata ctacaattcc taacataatc
       61 gtagaattta ataatttcca tagcataaag tgtttagaaa agaacattaa gaatagttct
      121 ataataattc ttgaactcca tgtcttataa tgttctatcg aagcacccaa atagccaagt
      181 tcatctaatc tacttgcata aactacgtca tccgctctca tagtcggcat atacatatta
      241 aagagtaaaa agatacccca aatccctatt agtaacaaga tcagttcttt gttattcgtt
      301 atttttctat tatgtttgtt catttttata ctccttctta tatttcctaa tagagtataa
      361 catagcttta agcattactt aaagataact taaaagttat cttttaaata cttaattgtt
      421 aaatacctta tttgatgcaa aagaaatcta cgatagtcaa caaaaaaatt ttcattatat
      481 ccagaaaaat ttttcttagt taaggaccta tggattgcag taatgcagaa ttcgtcgaat
      541 aaactgccaa agacatacaa tcatattgct tttaaaattg acgaacaaga tatagattcg
      601 tttgtctcaa aaatccaaat gttaggttta actgtcgaac caggacgttc tcgtgtgaaa
      661 ggcgaggctt cttcaattta cttttacgac tatgataatc acctcttcga gttacatacc
      721 ggtactctcc aggaacgttt aaaatcctac aattccacta cctgagacta gtaatgacta
      781 gtctcttttt atggcctctg atccccccat tgagcgaata ctaatagggc ttcgaattga
      841 tctgttcaat ctttaagcaa tatccctcaa atgagtttgg aaagtttagt gattcatcac
      901 aaatacttaa atacatgtag ttatatatag attgaggttc ttgtgagcgt atgctcgtgt
      961 gggttcaaat cccatcgtct gcatccatct ttccaaaaca agcggctagg acatttttgt
     1021 ctcagccgct tgttttctag tttattcttt catcttgtcg gacctttttt cttgggtata
     1081 gacttttctc atattctacc acaattttgg gtttccctaa cacatgatag gatctcacaa
     1141 caaattgctt tctattatta taataacctg taacgactaa cttcatccct tcgtctactt
     1201 cagctaggaa gttcaaacta tgggaagcaa tcaaacaatt gaccccattt aaactgaaac
     1261 ggatcaacgg tctggcacta acttgcaaag tacggatctt atctactgtt ccatattttt
     1321 tctccataac cttcacctca ataccattat acgaacatac attccctttg taaagaagaa
     1381 actctgtttt atctttttga ctttttgtct attacctttc ttaaaatcat attggcactt
     1441 cctgcgaaga tgaaggacat gtactaaaaa aaagaggcag cagtatagat tactgttgct
     1501 tctttactaa tttaggttta cttgttaaga aagattattt acgcaaatgt tcttcaacaa
     1561 tttgctgaga tttatcagct gtatcttcca cttcatcttt tgctttttct aacttttgaa
     1621 tatattgttt caagcttgag ttttcagcag ttgcttcgtc taatttcttt tgtgtatctg
     1681 ttaactgagt ttctacattt gttttttctt gtgtcagtgt tgcgatttgt tgattcaaac
     1741 catttacagt attttgtaat tctgaaagtt ttgcttgtgc ttcttgtagt cttgctgtcc
     1801 aatctgcatt cagatgattg atttcatcat ttttttggtt gatctcatct agtttgacat
     1861 tgatttcatt ttgtttgctt tgtagttgtg catccttgct gactaattga ctttctaagt
     1921 tttgtttttc tgctgccgat tgattttttt ggttttctaa ttcatttact tttgtttggt
     1981 attcttggac atctgccttt gaatcttgca atgtctgttc tttttgtgct agtcgagcgg
     2041 taagatctgt aagcgcttgc tctttatctg tcaatgtatg atcaagctgt gtcagattct
     2101 cattgattgt atcaatatct gtctctccac cccacaaaat aatttcattt gcgtaccgcg
     2161 tgcctgtgta gccaattaga catagaaatg caacgacaat cccaccagta ataaatttct
     2221 ttttcatttg catagctcct taatccaata cttttacatg ctaaaagtaa gtgatctcaa
     2281 acacaatgtc aagcgttttt aaattacatg tgtaatttta atataaatac tatcaaatac
     2341 tcatttaatc ctcctttttt atctatttaa aaaatctttt ttatagagaa aaatgcaggt
     2401 taatactttc ttaa
//
LOCUS       DACDEG010000180         2411 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.188, whole genome shotgun sequence.
ACCESSION   DACDEG010000180 DACDEG010000000
VERSION     DACDEG010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.188"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..296)
                     /locus_tag="IV142_002756"
     CDS             complement(<1..296)
                     /locus_tag="IV142_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8486331.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRI"
     gene            complement(627..860)
                     /locus_tag="IV142_002757"
     CDS             complement(627..860)
                     /locus_tag="IV142_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAP8486332.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(875..1825)
                     /locus_tag="IV142_002758"
     CDS             complement(875..1825)
                     /locus_tag="IV142_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP8486333.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(1822..>2411)
                     /locus_tag="IV142_002759"
     CDS             complement(1822..>2411)
                     /locus_tag="IV142_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8486334.1"
                     /translation="SNSVIKGDTAIGITFSSFLALGVILIGVANSSTDLFHILFGNIL
                     AVQDIDKWITIGVSVLVLVIVVLFFKELLITSFDPLMAKAIGMNVSFYHYLLMVLLTL
                     VAVTAMQSVGTILIVAMLITPAATAYLYANSLKTMIFISATVGASSSLLGLFIGYSFN
                     IAAGSSIVLTAAFLFVISFLISPKQKFLRKKERSL"
BASE COUNT          773 a          448 c          296 g          894 t
ORIGIN      
        1 aatatacgtt tattctcaaa aatataatta ataaatggat ctaattcatt gaagttctct
       61 ctccaacttt tctctatatc tatattttct aagtttaaga ggtatttggt aaactcttca
      121 ttatacatat taataagcgc attaatatgc ggctgaataa tttcttcaaa taacgcttcc
      181 ttagaagtaa aatgtttata aaaagcaccc gtagttacgc ctgcggtcag gcacagttgc
      241 cttatcttag tttgctgaaa tccttctttt tcaaaatggc ttagtccact ttctattaac
      301 ttttggtgag ttataatata atctctggtt tctatgggta cttcctccta ataagatgaa
      361 ttaactttat tatcttacac aggacttgaa gtttcaatcg ttaacgttta ttactgaact
      421 cattccttag agctaaacta atgagaaaga gcagaataac tgatacaaaa agcaatccta
      481 caagaattaa tagactcatc taaatcattc accttttcta aaataatccg agaaagattt
      541 aaaaaaatta tatccagcta ttaggataag tcctatcata atcactccaa tagccatttt
      601 cttatcctcc ctaaaaataa agtcttttac ttaaaacctt ttttgtaatc gtcaataaat
      661 tttttccctt tatcagttaa ttgaaatcct atatctaaat agtcctcata tctaagttta
      721 gccagcatct cacttacaga tgctggacta acacttagaa cttttgctaa atcattattt
      781 tttaatttct tttttctttt tgataattcc tgaattgcta gtaaataaat tagtatagta
      841 gtcgttttaa tttgtaccat acctaccctc caaattattt cgaaaggcct tcagcaattt
      901 tatccaggtt ccatttcatc atcgcatagt aactatcacc atcttgtcct ttttctgcaa
      961 ttgaatcagt aaaaatcgtt gaatagatag gaatattggt atcttttgat actgttttca
     1021 ttggtctatc atccacactg ctttctacga ataaggaggg aacttttgtt gtgcgtaatt
     1081 tttcaactaa gtgttttatt tgatctggtg ttccttcttc ttcagtgttg atttcccaaa
     1141 tgtaagcaga aggcacatta tacgctttcg agaaatattt aaagcatcct tcacttgtaa
     1201 caatcatttt tttatcattc ggaattgaag caaatttaga tttagcttct ttgtctagag
     1261 aatccaattt ttcaatatac ttatctagat tttctttata gaatttttta ttatcaggat
     1321 ctttttccgc taactgtttt tcaatatttt tagcgtaaat aataccgttt tctaaattta
     1381 accaagcatg aggatcttcc ttccctttct cactctgacc ctctaagtaa ataacatcta
     1441 tgccatcact tgctgcaaaa tagtctttgt tttcctcttt gttcgcattt ttaactaatt
     1501 ttgtaaacca agcatttcct ccagtttcca agttaacacc gttataaaaa atcaaatctg
     1561 cttttgaagt tttttgaaca tcttcaggca aaggttcata ttcgtgggga tctttcccaa
     1621 taggtacgat actgtgtaaa tcaattttat cttttgctat attttcggta atatctgcta
     1681 aaatcgaatt agtaactact acttttaatt tttcgttcga agccacttta tctttgtctt
     1741 tactatttat tgaacatgcc ccgaatataa aaagagcaat caaaatcccc agacttccca
     1801 gtactgcttt ccattttctc atcataacga tctctccttc tttcttaaaa atttttgttt
     1861 tggtgagatt agaaaactaa ttacaaataa gaatgccgct gttaaaacaa tacttgatcc
     1921 tgctgctatg ttaaaactat aaccaataaa caatccaagc aatgatgaac ttgctccgac
     1981 tgttgcagaa ataaatatca tagttttcaa gctatttgca tataaatatg ctgtagctgc
     2041 tggtgtaatt aacattgcaa caattaatat agttcccaca ctttgcatag ctgttactgc
     2101 tactaaagtt aatagaacca tcaatagata atgataaaaa ctgacattca tccctattgc
     2161 ttttgccatt aatggatcaa aagaagtgat taataattct ttaaaaaata gaactactat
     2221 aaccaacact aaaaccgaaa cgccaattgt aatccactta tcaatatctt gtacagctaa
     2281 aatatttcca aataaaatat gaaataaatc cgttgagcta tttgcaactc ctataaggat
     2341 aactcctagt gctaaaaaag aactgaaagt aataccaatt gctgtatctc cttttattac
     2401 actattgctt t
//
LOCUS       DACDEG010000181         2401 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.189, whole genome shotgun sequence.
ACCESSION   DACDEG010000181 DACDEG010000000
VERSION     DACDEG010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.189"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            207..2069
                     /locus_tag="IV142_002760"
     CDS             207..2069
                     /locus_tag="IV142_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8486335.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..2401
                     /locus_tag="IV142_002761"
     CDS             2069..2401
                     /locus_tag="IV142_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8486336.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          867 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
     2401 a
//
LOCUS       DACDEG010000182         2350 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.190, whole genome shotgun sequence.
ACCESSION   DACDEG010000182 DACDEG010000000
VERSION     DACDEG010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.190"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(138..428)
                     /locus_tag="IV142_002762"
     CDS             complement(138..428)
                     /locus_tag="IV142_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486337.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="IV142_002763"
     CDS             complement(546..806)
                     /locus_tag="IV142_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486338.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="IV142_002764"
     CDS             complement(943..1356)
                     /locus_tag="IV142_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP8486339.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="IV142_002765"
     CDS             complement(1812..2297)
                     /locus_tag="IV142_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486340.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
BASE COUNT          861 a          381 c          369 g          739 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag
//
LOCUS       DACDEG010000183         2260 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.191, whole genome shotgun sequence.
ACCESSION   DACDEG010000183 DACDEG010000000
VERSION     DACDEG010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.191"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(1..345)
                     /locus_tag="IV142_002766"
                     /pseudo
     CDS             complement(1..345)
                     /locus_tag="IV142_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            620..1162
                     /locus_tag="IV142_002767"
     CDS             620..1162
                     /locus_tag="IV142_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486341.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            1318..1767
                     /locus_tag="IV142_002768"
     CDS             1318..1767
                     /locus_tag="IV142_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAP8486342.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMAPIALDNEHPRI"
     gene            complement(1797..>2260)
                     /locus_tag="IV142_002769"
     CDS             complement(1797..>2260)
                     /locus_tag="IV142_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8486343.1"
                     /translation="LILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTD
                     RGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQL
                     RLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          777 a          354 c          426 g          703 t
ORIGIN      
        1 tatttagaat tttgcacatc gctgatatgg aatatttatg cttgttggca tcaattactt
       61 gtcttttcgt ccgaatatca gcgccgcttg ctttaaaata tcattctcca tttcgagttg
      121 cttatttttc tttcttagtg cgatcaattc tgcttgttct ggtgttaagt tgtctctttc
      181 tttgaatgag cccgttgatt gtgcttgctt catccattta tcgaaagaag aaggcgtaag
      241 ctcatactct cgaataattt ctgcgcgagg cttaccagcg agatagagat cgacgatttg
      301 ttgtttgaat tcttttgaat aggttcttcg ttgacgtctt ggcatgaaaa atcctccagt
      361 gtgtttttta ttattctaca caccttattt tttctgtcta gtctagtgta gccgattcaa
      421 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
      481 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      541 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      601 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      661 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      721 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      781 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      841 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      901 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      961 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
     1021 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
     1081 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
     1141 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
     1201 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tcttgatgaa
     1261 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
     1321 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
     1381 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1441 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1501 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1561 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1621 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1681 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc tccgattgcc
     1741 cttgacaatg agcatcctcg gatctgattt tctccggcag aagcacaatc attcaattac
     1801 gctgcctctc cagaggtatc agatccgcgc tcattatcaa ggattcgctt cgccaatctc
     1861 tgttgtcgaa tttggatcgg tgtttcatac gccaacgtgc catgtaagcg tagatagttc
     1921 caccaatgca cataatcaaa caattctaga cgtagctgtg ccagtgtctc aaattggtat
     1981 tgatgcacga attctacttt gacagattta taggttgatt ccacaacggc attatcatac
     2041 ggacaaccct tcctactcaa tgaacgagta attccaaaac cattcagaat ctcatgaatg
     2101 gtttggttat caaattcctt tccacggtct gtatgaaaaa gcttgacgtc tgttaaagaa
     2161 tacggtatcc gtccaaaggc ttcttttacc aatgaggcat ctttcttctc accacaggaa
     2221 taaccaataa tttctcgatt aaacaaatca agtattaagc
//
LOCUS       DACDEG010000184         2230 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.192, whole genome shotgun sequence.
ACCESSION   DACDEG010000184 DACDEG010000000
VERSION     DACDEG010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.192"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..376
                     /locus_tag="IV142_002770"
     CDS             <1..376
                     /locus_tag="IV142_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP8486344.1"
                     /translation="NVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFRR
                     FPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTDD
                     WQKVMAAEVIATYLDGDLVYKK"
     gene            424..1824
                     /gene="yfcC"
                     /locus_tag="IV142_002771"
     CDS             424..1824
                     /gene="yfcC"
                     /locus_tag="IV142_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP8486345.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1857..2024
                     /locus_tag="IV142_002772"
     CDS             1857..2024
                     /locus_tag="IV142_002772"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486346.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAFTGSVAKF"
     gene            2078..>2230
                     /locus_tag="IV142_002773"
     CDS             2078..>2230
                     /locus_tag="IV142_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8486347.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVREILYDRGINVSH
                     TTIYRWV"
BASE COUNT          626 a          371 c          483 g          750 t
ORIGIN      
        1 aaatgtttcc acagaggact tctttaatta ttgtcgatta gaggaaagtg gtgtaacagt
       61 cactagcggt acagatttac cgctgtttat tactgatatt cctgaatcga ttataagagc
      121 agtattccgt cgttttcctg aaggagaaga agtctgggag aaagaaacaa gcttcacgac
      181 aaccgagctg ctgaaaagtt ttacaagcaa cagcttcaaa gtaaatggtt tatcggagta
      241 cggagaaata aaagtaggga aaagcgccag cttagctgtt ttcagcgagg acctctgtac
      301 tgatgactgg caaaaagtga tggctgcgga agttatcgct acctatcttg atggtgactt
      361 agtttacaag aaataatgct tttcgatata acgttataaa acgaattaaa gagaggttaa
      421 aaaatggaaa caatgaaaag aaaattcacc ttcccacata gttatacatt gatttttatc
      481 ttaattattt tggctgcaat tttaacttgg ataattccta gtggtcagtt tgagacagtt
      541 gttgaaaacg tcgcaggtat ggaaaaaaca acggttgttc cgggaaccta tcaccaaatc
      601 gataaggcag attatggaca aggtttttcc tctatactaa tggcaccagc tgaagggatt
      661 atttcagctg ttgaggttgt tgcttttgtt ctcatcgttg gtggtgcctt cgggattatc
      721 ctaaaaacgg gagccatcga tcgagggctg tttaccttag caaaagttct gggagacaag
      781 gggatcttag ttattccgat gtcgatgatt ctattcagct taggaggatc aacctttggc
      841 atgagcgagg aagtcattcc cttatttgcg gtgtttattt ctctgatgtt ttctcttggc
      901 tatgattcca tgactgcgat cttgattcta tttttaagta cacaagcggg atatgttggg
      961 tcgacgatta atcccttcaa cgttttaatc gcacagggag tagctggcgt ttacggaaat
     1021 cccctgcttt ggtttcggat gctatcctgg attgtcatta ctgggatttc aattgttttc
     1081 accatgcttt atgctagcag agtcaaaaag aatccactgt cctctattgt ttatgagagt
     1141 gatttaaagg ttaagcataa gtttcaaaat tttgacactg aaaaagagtt ttcaacacaa
     1201 gataaactaa ttatcgccgg ttttatcgta ggtttaggaa tcatgatttg gggtatcatt
     1261 tccaagggat ggtatatggt tgaaatttct gcactattct taggattagg tctttttgct
     1321 gggggcatcg ctagattttc tcaaaaggaa attgctgaag cttttgtcga aggctgttca
     1381 gatttcgcct atgctgctgt aatcattggg ctagctagag gagtcttggt aatcttggaa
     1441 ggtggaatga ttattgacac tattctaaac agcttggcta accttttagg tggtcttcca
     1501 aaattcctgt tcactacgat tctactcatt tctcaagtaa ttgtttcctt ttttgttcca
     1561 tcctcctcag gagctgcggc attaacgatg ccggttatgg caccattggg tgatctaatg
     1621 aatgtaagtc gtgatgttgt tgttctaagt aaccagtttg gaaatggttt aatgaatatt
     1681 atctccccaa ctagtggtgt attactggca ggattagcca tcgcagagat taatttctct
     1741 aaatggttta aggttggctt caaaatattt gccatcttaa cccttgtttc tgctgttttg
     1801 ttatacattg ctacactctt ttagtcttaa tcatccatta ataggaggca atagttatga
     1861 ttaaaataaa tcaattagca ggaatgaata tccactattt attttattct ctcaaagaat
     1921 ttttcgctta tcaacagaaa attggtattt cttctgttga actttgggga ggtgctccgc
     1981 attttttcat agatgctttt actggttctg ttgcaaagtt ttaaatctac tatcaaataa
     2041 ggtagaataa tagaaaaaga tagcaggagg aatgacgatg aatcatttta aaggaaagca
     2101 atttcagcag gatgtgatta ttgtagccgt gggctactat cttcgttata accttagcta
     2161 tcgtgaagtt cgagaaatct tatatgatcg tggcattaac gtttctcata cgacgattta
     2221 tcgttgggtg
//
LOCUS       DACDEG010000185         2211 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.193, whole genome shotgun sequence.
ACCESSION   DACDEG010000185 DACDEG010000000
VERSION     DACDEG010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.193"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(637..753)
                     /locus_tag="IV142_002774"
     CDS             complement(637..753)
                     /locus_tag="IV142_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP8486348.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            complement(1229..2107)
                     /locus_tag="IV142_002775"
     CDS             complement(1229..2107)
                     /locus_tag="IV142_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486349.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
BASE COUNT          695 a          385 c          362 g          769 t
ORIGIN      
        1 ctattatctg aaaaccaaaa cttattattc cttgatgtta ttctagcaat agtaactgct
       61 tttttatttt tttgcatatt ttttcggcgt tactttttaa agttaacttt ttcgacaaat
      121 agaaagagat acgaatcact gatttttttg aagaaaagaa atggagtgag ctgaacgtta
      181 gagatattgg gagctttcgt aactgcttat taaacgataa tggttatttg caaccgtatt
      241 gctcgcttct acatgaaata tttcatgaaa tatttcgcaa aacctttgat tatgtagtta
      301 cgtttccatt cttgactaaa aatgtttcaa aaacgaacaa aggtgttaaa aggtaaagtg
      361 atcaatacta ctggtcaaaa taagaggttg aaagggaatt gaaaaataac tacagataat
      421 acttttataa acgtacgtgc tttgtggttc tgtagttaac tatataactg gaattaggga
      481 atggtatcaa tttgaaaaga aagattctac caatttatca tatgttaaaa atggaaaatc
      541 aacagcagtt ctttcggaaa ccttacacaa aaaccgatta ttgaaaatag ccgaaaaacg
      601 tttttcggct atttttttaa ttaagtttcc ttcttcttac cttttcccac tattccgttt
      661 cacatctttg atgatcttca gcgcacgttt tcgtgttttg atatttgggc ttttcaagcg
      721 gcggtatgca cttgagatat ctgttttttc catatggacg acccactttc ttctttgatt
      781 gtagtcatgc taaaaaacac aaatcgaaac aattacgatt tgtgttttaa atggtatcaa
      841 aagttaaaat gaaagtcaac ttgttaaaac agtcgatcag actagctgat tgactcctat
      901 ctctggaaat aaattttcat tattacgttt tagctaccta aaaaaggccc tcttgtccgt
      961 taactttaat atctattgtt atagctagtt tacctattga acgtactgtg tgttcgatag
     1021 aacactaaaa aagaggctgg gacagaagcg tttaactcca agaaataaga agaaattcac
     1081 gaaaattgct tttcaaattt ttgtgaattt cagcttattt ccgaaggagt tgcttctgct
     1141 tccgccgttt atacgtgttt agagcgtgag acaaaagtgt tctttacttt tgtctcacgc
     1201 tcttttaaac tatttcgaag tactaaactt atgaagtaag aatacctaag ccagagccaa
     1261 tgtgctcgcc tgcaagcaat tgctcttttg cttgtcgctg taaactcgta ctttctggat
     1321 aatcatgtat ttgggcatct actcgtccaa agatccaagg gactccacta tcgtcctgtt
     1381 catcaactga taataatcga taatcggctg caaaaagaca gatttttgac tcttttttca
     1441 accatttagt aagattgggt gatagtaacc aagtatctgt gtacatcact tggtaatggt
     1501 aatcaggaaa ataaagttca aaaaaacgaa tggcactttc gtaactatct cttatcttat
     1561 tctgagataa atctgcatcg gaaggaatat gaagagaaag gcactgcttt ccttctaatc
     1621 ttagatccgt gtaccctttc ggaaaatatg tcatttcgta ctccaattca ccaatccgaa
     1681 agatcaagcc actagtgtaa tgccaaatcc aaaaaccacg gtcaaagcga tagcttcctg
     1741 ttacttggaa atgttcatga ataaatctag taaaagcatt cattgttgcc aaatagatag
     1801 ttcttggaat cttcaatggt tgataaattt ttttccaact ttgagaagct gcatatagat
     1861 aaatagctaa tgctgcaaaa ggttcctctt catagacttt gtctattttc tttgcagtcg
     1921 cctttgcttt attaggatcc attagttcat gactatattg ctcaaacacc tcatcaagga
     1981 tgttttttgc ttgtacaatc atttgctcta taacgacttt gggaagagtt aaagtttctg
     2041 ccagaatttg gatttcattg cttattttct tcatcaaatt tccccttttc atatctttga
     2101 acctcattgt ttaaatgact gattatccgg acaccatatg gtataaacat acttttattg
     2161 tacactaact ataaaactta aactttctat gaagaaagaa tatctgtaag a
//
LOCUS       DACDEG010000186         2200 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.194, whole genome shotgun sequence.
ACCESSION   DACDEG010000186 DACDEG010000000
VERSION     DACDEG010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.194"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(118..708)
                     /locus_tag="IV142_002776"
     CDS             complement(118..708)
                     /locus_tag="IV142_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP8486350.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
     gene            1231..1392
                     /locus_tag="IV142_002777"
     CDS             1231..1392
                     /locus_tag="IV142_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486351.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLA"
     gene            1411..1905
                     /locus_tag="IV142_002778"
     CDS             1411..1905
                     /locus_tag="IV142_002778"
                     /inference="COORDINATES: protein motif:HMM:NF018154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin"
                     /protein_id="HAP8486352.1"
                     /translation="MIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGYSF
                     ILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEMFI
                     NAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPIQYTSKELEE
                     ELHD"
BASE COUNT          802 a          337 c          373 g          688 t
ORIGIN      
        1 tatgaatata aatagagtgt atcttctttg ataaatgtca gaaaaagtgt gacaaataac
       61 catcagtaaa attacttgtc atactattga taaggagtat ttaattgaag agaatgatta
      121 ggagaaagga tctccgtact ttttatagta atcatagaat ccaagtttgt atagacgaat
      181 ctttttttct cttatttctt ctgtttcaaa atggactgct tgaagaatca agtctgtgaa
      241 ttctatagga gctgttccat tcgcagtaat caaattacga tctttcacag cttgttgttc
      301 tataaaatct tctttattct gataattttc cgattctaac caaggaaatt gcgcatttcc
      361 ggtgtgtttg taaccttgca ataatccgct ctgagctaaa taatcaactg ccccacaaat
      421 agcagctact ggtacgtctg tttttaacaa tttatcgatt atttgatata aatgatgatt
      481 ttttatgtcc catgaattgc caccgatcat aataaaaagg tcaacttttt caggtatttg
      541 ttcgattaag taatcgattt ttgtttttaa tcctccaatg gaagtgacct ctgattggat
      601 agaagcagtt ttgttttgcc atccctcttt ttggtttagt gtgctagtaa tataagcacc
      661 ttcccaatcg gcatattcct ctaatagaaa cgtaatcgca gttttcattt tcaagctctc
      721 ctttactatc tatgacaagg acaacagttt taacatacag acattcatta aatttggata
      781 gcgacagcga aaacaacaat tcttaagttt tagacagagt atacagacag tcaacttaat
      841 taaatacgaa ctaaataaaa atgacaggta atcatttttg tttaccagca ttataacatt
      901 ttgcatacat ggatgacaaa agaaccaatt cccacttgct tgagtacaat cttaaaagaa
      961 actggaaggc aaaaagtaat tatagaaacg aaaaataaat catatcgggc actcagacat
     1021 gaaaacaacc ctagaaatat ataactagtc caatgaaaaa aaagcgaatg ccgtagaaaa
     1081 caaggattct gcgtacattc atatctcatt ttgcgagcat atctgctatt actgtgacta
     1141 aatgttaata tggttaaaat gaccgttata ttttttatat aaatatcgta taattagtat
     1201 gaatatagaa gaaggaaaga aggtgcaatc ttggaaccta aagacaagta tttagattac
     1261 gctagggtca ataaagataa atttattgaa acaatcatca agggaaaaat ccctgatgat
     1321 gaaaaagatg cggtattcat ggctggaagc ccaggagcag gtaaaacaga ggttgcacta
     1381 ggtttagctt aaaattatga caaccatgtt gtgattgatg cggactattt tagagcacaa
     1441 tttccagaat acaatggaaa gaactcaagt gtttttcaga aagcttcttc ttggttagtt
     1501 gaacaatcat taaaatatgt actggaacat ggctattctt tcatactaga ggatactttt
     1561 gctatcctta gtgcagagaa aaatattatt cgagccctta aaaataactt tcgcgttacg
     1621 attttctatg tttaccaaga tcccaaagtt gcctgggatt tcactcgaaa acgtgaacta
     1681 gctgaaggac gtcatgtgcc aaaagaaatg tttatcaatg cgttttttaa gtccagagag
     1741 aatattgaaa aagtcaaaaa tagacatcct gaagtagtcc ttcacataat gattaaggat
     1801 tatcaaaacg acatttctga agtgcattat gcagcagata atattcaatt agtactacca
     1861 atacaatata cttcaaaaga gttggaggag gaattacatg actgagaaag aacaagtaca
     1921 acaaatcgtg aagaagtata ataaaagtat cgctgattta tcagaaaacg cctcagcgaa
     1981 agaatttaaa acagtaatga attacgtagc agatgaagca aatcgtaagc agcgaaaact
     2041 agttgggttg gataaataaa ttttttaaac catatccttg taaagttgag ttttttattt
     2101 taatgaatta agactattaa atagataatt attgtaactt aaaaaacgag agtataaaat
     2161 attttgtgta aatgaaaaaa tccatacaaa aaaggaagtc
//
LOCUS       DACDEG010000187         2180 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.195, whole genome shotgun sequence.
ACCESSION   DACDEG010000187 DACDEG010000000
VERSION     DACDEG010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.195"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            700..>2180
                     /gene="ltrA"
                     /locus_tag="IV142_002779"
     CDS             700..>2180
                     /gene="ltrA"
                     /locus_tag="IV142_002779"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8486353.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKP"
BASE COUNT          858 a          336 c          455 g          531 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc
//
LOCUS       DACDEG010000188         2163 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.196, whole genome shotgun sequence.
ACCESSION   DACDEG010000188 DACDEG010000000
VERSION     DACDEG010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.196"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..293
                     /locus_tag="IV142_002780"
     CDS             <1..293
                     /locus_tag="IV142_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP8486354.1"
                     /translation="SSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETA
                     LNKIQDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            266..943
                     /locus_tag="IV142_002781"
     CDS             266..943
                     /locus_tag="IV142_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP8486355.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            933..2075
                     /locus_tag="IV142_002782"
     CDS             933..2075
                     /locus_tag="IV142_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP8486356.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
BASE COUNT          798 a          286 c          435 g          644 t
ORIGIN      
        1 attcttcttc tgggataatt gaagctgcat cattcaaaat accagtaata aatttaggaa
       61 ccagacaaaa tggacgagaa caatctaaaa atattataaa tgcaaatcta aatgaaaatg
      121 aaattgaaac tgctttgaat aaaatacaag atcctcagtt tataaaaagc ttgaaaaatg
      181 ttaagaacat ctacggtgac ggcaacagtt ctagaagaat aacaaaaaca ttgaatcaaa
      241 tattgaatga tggggaagat attaagtgga ttcaaaaaca aatagcttat taaaatatac
      301 tagtgatcca tacattatta ttggggctgg cggacacgca aaagtcatta ttgaaacctt
      361 aagattagaa aataagttaa tatatggatt ttttgatgat aagcaaaata aaatgcaaga
      421 tctgaaatat cttgggaaaa taaacgaatt gaatagctgg gcggatagca aaaaaaaaat
      481 ctataaattt attgtagcta ttggtgactg tgtagtaaga aaaaagatta taaaaaaatt
      541 gaatatgcca agtgagcaat atggaatagc tgttcatcca agtgctatag tggcaaaaga
      601 aatttcaatt ggttatggaa ctgttatttt tgcgaatgct gtaattaacc cagatgctgt
      661 aattggagag catgctataa taaacacagg tagcattgtt gagcatgatt gtagaattaa
      721 taactatgtt catttatctc caggtgtatg cttagcgggt ggagtacatg taggtgtagg
      781 aactcaaata ggaattggtt ctcaatgtat ccagatgaag aaaattggat cttggtgtat
      841 gataggcgca gggagcacta ttgttaagga tattccctca cattccttag cttatggcaa
      901 tccagctaaa attaagaaag aaggaattaa ttttgaattc tagaatttta ttatcaagtc
      961 ctcatatggg aggaacagaa gaaaaatatg ttgctaaggc ttttaaagat aattggatag
     1021 cgccattagg tgaaaatgtg gataaatttg aagaaagtat tacaaaatat actggttcta
     1081 agggcggagc ttctgcgtta agttcaggta cagctgccat acatcttgcc ttaagactgc
     1141 ttaatgttgg aaaaaacgat attgtctttg ctccttcatt tacttttata gctacagtaa
     1201 atcctatttt atatcaaggg gctgtaccag tgttcattga ctcagaacct aatacatgga
     1261 atatgagccc tgttgcacta gagagcgcat tagaagaagc tgaaagaaac aaccaattac
     1321 ctaaggcaat catcgtagtg aatttatatg gtcaaatggc tgattatgaa cgaattttag
     1381 ctattgcaaa ccaatttaat gttccagtga tagaagatgc agcggaatcg ttaggttcta
     1441 cttataaggg aatttcgtca ggcttatttg ggacaattgg gatctattca tttaacggta
     1501 ataagatcat cacaacctca ggtggaggag ctctagtgtc tagtgataaa aaatatgttg
     1561 agcattcaag atttttagca acacaagcaa gagatcaagc accgtattat caacatagcg
     1621 aaataggtta taattaccgt ctaagcaata tttctgccgg tatcggacgt ggacaaatgg
     1681 aagtattgaa tgatagagta gctaaacgaa gagaaatttt caatcgatat aaggaagctt
     1741 tttctggaat tgatagaatt gaaatgatgc cagaagtgga tggcatggtg ccaaatcgtt
     1801 ggttgtctac tttaacgatc aatgattata attctattga tcaagtagtt aacgtaataa
     1861 ataaactaaa tgaaaaaaac attgaaacaa gagctttgtg gaaaccacta catttgcaac
     1921 cagtatttca aggggtaaaa atgtattacc atgataataa tcttagtgtg tcagaaaagt
     1981 tatttaatag cggcttatgc ttaccttctg gaagtaatat gacagatagt gaacaagaat
     2041 ttgttattga tgagtttaaa aaaatattca agtaggtgtg atattttatg aacataggaa
     2101 ttattggttt aggttccatg gggaaaagaa gacttagact cattcaagaa cactttaatg
     2161 ata
//
LOCUS       DACDEG010000189         2097 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.197, whole genome shotgun sequence.
ACCESSION   DACDEG010000189 DACDEG010000000
VERSION     DACDEG010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.197"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>2097
                     /gene="acm"
                     /locus_tag="IV142_002783"
     CDS             <1..>2097
                     /gene="acm"
                     /locus_tag="IV142_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP8486357.1"
                     /translation="IPVHVYADAGRDISSNVTSLTVDPTNITDGGNIKVKFSFDEKKQ
                     NIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRKDSAQVVFNENIKNLD
                     SVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPASGSSSSVFYYKTGDM
                     LPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEINQIHLGEQKVYRGE
                     EGIQQFLQDFPGATFNFSVTDNYIEITIPKNFVNLRKIMVSYKTIIENPEQINFENHS
                     EAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIFKYINDTEIGIPNVTFEL
                     RRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPNWIDFDPLSSNELKFS
                     INENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLFRASTNNWEPVPDAET
                     KRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGYQKIENGLVVTNENK
                     EVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEKDDWRYRFTNLPKYR
                     DGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHITPWKNFPEKTEREK
                     IRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKSILPKTSSKKSSFAVIIG
                     LLIVTICAGIFLQKYK"
BASE COUNT          821 a          343 c          412 g          521 t
ORIGIN      
        1 tgattcctgt tcatgtctat gcggatgcag gcagagatat cagcagtaat gtcacttcgt
       61 tgaccgtgga cccaacgaat attacagatg gaggaaacat aaaagtaaaa ttctcgtttg
      121 acgaaaaaaa acaaaatatc caaccaggtg attatctttg gatcaattgg ccaagtgaag
      181 gaaatattcg cggtgaagga ttccaaaaag aaatcccatt aatgattgaa aataaaaatg
      241 ttggaacttt aacggtcaga aaagattctg cgcaggttgt gtttaatgaa aatattaaaa
      301 acctagactc agtagaaggt tgggggcaat ttgagataca ggccagaaac gtaaccgata
      361 ccggtgaaga aaatacggga ccattcaccg tgacgagcgg tgataaaaca gctactgtaa
      421 atgtgacaaa gccagcttct ggttcttctt ctagcgtttt ttattataaa acaggagaca
      481 tgctacctga agatactaag cacattcgat ggtttttgaa tattaacaat aacggcactt
      541 atgttgaaca accagttaaa atcagtgatg aaatccaaag tggacaaaga ttagatccaa
      601 gtacgtttga aattaatcaa atacacttag gtgaacagaa ggtttataga ggagaagagg
      661 gaatccaaca atttttacaa gatttccccg gcgctacatt taatttttca gtaactgata
      721 attacattga aataactata ccaaaaaact ttgtcaatct taggaaaata atggtgagtt
      781 ataagacgat catagaaaac ccagaacaaa taaattttga gaatcattcg gaagcttggt
      841 ttaaagaatt taataaacct gctgttgatg gagaaagttt caatcataca gttaaaaata
      901 tttcagcttc tggtggagtc aacggtacag ttagaggcga attgaagatt ttcaaatata
      961 tcaatgacac agagattggt atcccaaatg tgactttcga attaagacga gcagatgaac
     1021 agcctattca aggacagtct agtatcctgt tgacttcgaa tgaacaggga gaagctagta
     1081 tcaaaggcct gcaagtagga gactatgttg taaaagaaaa agaagcaccc aattggattg
     1141 attttgatcc actaagttca aatgaactca aattctccat aaacgaaaat gatacagagg
     1201 gagtcagttt acctatttat aacaagaaaa aggttacgaa catcacagca actaaaattt
     1261 ggaacggtgg aaccactcca agaccgagta tttattttaa attattcaga gcaagtacaa
     1321 acaattggga accagttcct gatgcagaaa caaaacgatt agacaatgga ataacttcag
     1381 tgacttggga agatatccaa cagtatgatg atagtggaaa cgaatatacg tttaaagtgc
     1441 aagaagtaga tcaaaacgga aatgattatg tcccttcagg ttatcaaaaa attgaaaacg
     1501 ggctagtcgt tacaaatgag aataaagaag tcatttcagt ctcaggacaa aaaacatggg
     1561 aagacaatga gaaccaagac ggcaaacgac cgacgacgat cacagtaaat ctattagccg
     1621 acggtcaacc gatccaacac aaagaagtaa gtgaaaaaga tgattggcgc tatcgtttca
     1681 caaatctgcc aaaatacaga gacgggcaag aaatcatcta tactgtaacg gaagacaacg
     1741 tgcccgaata cagtacgacg attgacgggt ataacatcaa aaacagttat acaccggaaa
     1801 aaacaaatat atctatacca ggtagccata taacaccgtg gaaaaatttt ccagagaaga
     1861 cagagagaga gaaaattaga aatctattct ctcaaaaatt gcaaatattt gctcatacag
     1921 gagaatccag cgaacaaaga agcaattata ttagtaagag agctgtacct aaacagataa
     1981 caaataaatc aaaaagcatt cttcctaaaa caagtagtaa aaaatctagt tttgctgtaa
     2041 tcattggttt attgatagta actatctgtg ctgggatatt cttacaaaaa tataaaa
//
LOCUS       DACDEG010000190         2061 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.198, whole genome shotgun sequence.
ACCESSION   DACDEG010000190 DACDEG010000000
VERSION     DACDEG010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.198"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(71..226)
                     /locus_tag="IV142_002784"
     CDS             complement(71..226)
                     /locus_tag="IV142_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010001600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8486358.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
     gene            843..1064
                     /locus_tag="IV142_002785"
     CDS             843..1064
                     /locus_tag="IV142_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486359.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            1065..1322
                     /locus_tag="IV142_002786"
     CDS             1065..1322
                     /locus_tag="IV142_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486360.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKAAKSAVPTGFLTKQPYIF"
     gene            complement(1458..2060)
                     /locus_tag="IV142_002787"
     CDS             complement(1458..2060)
                     /locus_tag="IV142_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP8486361.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKIRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYA
                     MSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
BASE COUNT          679 a          465 c          314 g          603 t
ORIGIN      
        1 tcatgctata atcaccttaa ttgaacaaca gtctaacagc aaaagcagtg cgccaacact
       61 aatgcttttg ctattttttt tgctcatttt cctttccgtt agcattaacc caaattgtaa
      121 taagaccaga cttactaaga cccatttgtt tcgcaatttt atcaagatta tctgccactt
      181 ccttagtaag cgttacaacg actctctttt tttcattact gatcataatt cgtatcctcc
      241 aacgactttt tcttaaaaac catagtaaca aaaaagtaac taaggtgcaa cagtttagat
      301 actaattagc gaataatagg ccttaaaccc ttggcattac tgattttgtc aaaaaaaaac
      361 gctgaaaccc ttgggagagt aaggctaaac agctatcgac caatgaccaa tgaccaataa
      421 ggggggtgtc gtagtccccc cctcctccgc ccaattcata aacaccaaaa ccaaaaaaca
      481 aacctcaggg ctccgccccg aaccccgacc tccttctacc gaaatatatg caggcgccac
      541 gcttactatt tcgttcagct atcggctttt gcaatctaac acgaccgttt cgctaccagt
      601 cgccttacag gctccattct cgcttctctt acgtgtttta acgattgcgc cgataactaa
      661 acgaaataga cgctaaatcg cttaaaacgc aaaatagacc ctttttagag cattgatttt
      721 ttctactagt catttcgata atataaaatt gaccacaaca cagaaaactt atattataag
      781 cttatgtcgt aagtccgacg tactcacgta atatagacgg acgttaaatg gaaggagcat
      841 aaatgagagt gataaaatca gaaaaattca ctttggccga aattatgacg accagcgaac
      901 ttgcacaaga acttgattac aaccaagctt acgttttgcg cttagcgaaa gaacatctga
      961 ctgaaggaaa agaatatcgc tctgccggta gaagaaacta tcttttcagc ccagaagccc
     1021 ttaaaaaact agaaaaagtc ataaccacca aagaaggtga gtaaatgcct aaagaaacaa
     1081 ctatcaacgt tgaacaacta gaaaaattgc tagaatcata ttccaatgat tttatcgaat
     1141 tgaccggcgg aaaagtcgaa gaaaccaccg acctctttga cctacttctc acaaatattc
     1201 tggaattaaa ctctgaagaa caaaaagagc ataagggaaa actgatagca ccttcctaca
     1261 ggaaggcggc taagtcggct gtgccaactg gttttcttac aaaacaacct tatatttttt
     1321 gatatcacaa aaaaatccat tttggaccta ttttcggtca taatatagta cctacttttg
     1381 gtcatagttt cgtccgtagt tttggtcata gtgactaaga ctttcacgca caaaaaaaga
     1441 gctgaaatta acagctctta tcctttattt atttttgaat ttacacttcc accatgacga
     1501 ttcgatttta tatattgccc gataatataa gctgaattat cacaaagaag cggaaaccaa
     1561 tcatcaaatc tttctgacat tgcatagtca attaaatctt tgatttcagt gatattctta
     1621 gaatcaacaa acgacatcat ctctctaatc aactcatatc gttctgcact cgttactgac
     1681 aaataactag caatttctgc accaccatga gcgataatat cacttttagc atattgaaat
     1741 ttttctggat tatccatatg agcaaaatat cgcaccatac cttttgcatt agcacatttt
     1801 tgcgggtttg gagaccgaat cttttgagtt atctcacgta tttgatcata cgtcttttta
     1861 ccgctaaaca ttatcaaaac atgccaatga ggctttttca attcaccgtc aggattcaca
     1921 tctttatcat gaagtggact ctcaacccaa ggaacatgta actcatctaa aaactccctc
     1981 caattttcag gagccgattc aggatagact acaaacgtcc aattccttgt gcgttcatct
     2041 ttagtttttc tactcgtcat g
//
LOCUS       DACDEG010000191         2050 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.199, whole genome shotgun sequence.
ACCESSION   DACDEG010000191 DACDEG010000000
VERSION     DACDEG010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.199"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(365..1657)
                     /locus_tag="IV142_002788"
     CDS             complement(365..1657)
                     /locus_tag="IV142_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8486362.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
BASE COUNT          661 a          396 c          328 g          665 t
ORIGIN      
        1 tatgggctac tctgtgatac ttattagcca ttaaactatt ttacccacat agcatatatt
       61 ttgaaatcgt tcattccgta aattcaacta aatatgccta aaaaaataaa aaatctaaga
      121 tagaataaga tttattcagt cttagatgga tttatagcaa ctttttcgta acaactttaa
      181 aaaatacgaa aagaaagagc tgttttactc tgactacgtt gctaaattgt cgtttaatca
      241 ttcatttaaa ccaaatcttt ttttatagaa ttcgttttaa tcctcctgaa tccaaaaact
      301 atgaaaaccg taattaaata gttaagagtt tttaaccacc ataattctct cggctatttt
      361 gaaatcagat aaattttgat cggacacata gttgaataaa cctaccgttg ttacggaagg
      421 taaatcgcat acttttcatt ctagaggaga aggtctcgtt gatcattaac tgattaagca
      481 ccatagccca gttttgaatt cgaccacctg accacttcgc gtgtaattct ttcgtgcgta
      541 aataaagtag ttttaggagt gcattctcat tagagaacgc tccttttttt gtgacttttc
      601 tgaagctgga gtggacactt tcaaccgcat tggtagtgta cataattttt cgaatggcac
      661 taccataatc aaatagttgt tcaacatgtg caaagttccg tttccagaca tctacagcac
      721 cagaataatg agaccaccga ttttgaaaac tgtcaaaagc agcatgtgca gcgtttagag
      781 aagaagcacc gtagaacttt ttcatatctc ggcagacttc cttatagtcc ttacttggaa
      841 tatagcgcaa tgcatttcga acaagatgaa caatacagcg ctgaacaatt accgatggaa
      901 agatcgcttt tgcgccttct tcgaggccag aaacaccgtc catcgaaatg aaaaagacat
      961 cttcgacacc acgtgctttc agttcatcaa atacttgcat ccagcgattt ttagattctg
     1021 tttgatttaa ccataatcct aaaatctcct tatttccttt gagatcatag ccaagaatgg
     1081 tgtatacagc atattctttg gcttcataat tttctcgtaa agtaacatac atacaatcaa
     1141 cgaatagaaa ggcataacac tttgctaggg ggcgggcttg ccattcttcc aattcaggaa
     1201 ggacagcgtc agtgatatct gaaatcattt catgggaaat atcaaagcca taaatagctt
     1261 cgacggttgc ggcaatatct cgttgactca ttcctcgtgc atacatggaa agaaccttcc
     1321 cttcgatgtc ggagacatct cgttttctct taggaattaa ctctggttca aaggaagctt
     1381 cccggtctct aggaacatca atagctactt caccaaaact ggttttaagc gttttagttc
     1441 catagccatt tcgacggtta tcgtgttcct taggctcttt agaatgggca tcataaccta
     1501 aatgattatt caattctcct tgaagcattt tttcaaaaag gggcccaaac acatctttca
     1561 aagcatcttg catgtcatcg acagattcag gttgataggc attcagaatg gattcagcta
     1621 acttttcggc atcaggattt cttttctttc tagccatcgt gtaatcacct cttgatctta
     1681 ttgtagaaaa agaaaaaccg cgaagcaacc acctgcctag gattaatggt tacttacacg
     1741 gtttatatta cactctcatc attaaaaaag tatctttcga tattactaaa caatgaaatt
     1801 atctatttga aatttaaatc tgataatata ttgagtgttc caatgagggc tttgatccta
     1861 ttatccatat ctttttcagt tttaatattg atacacatta taagaaaaag aaacttttgg
     1921 ttaatttatt tcatcccagt gcaaaatata aaaaagtaaa atagaccata ggcaatagat
     1981 taacaattaa tataatacaa gcaaatattc tattcattgt atttctgctt tcaaaaagtt
     2041 ctaatattga
//
LOCUS       DACDEG010000192         1820 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.200, whole genome shotgun sequence.
ACCESSION   DACDEG010000192 DACDEG010000000
VERSION     DACDEG010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.200"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="IV142_002789"
     CDS             complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="IV142_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP8486363.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(1305..1709)
                     /locus_tag="IV142_002790"
     CDS             complement(1305..1709)
                     /locus_tag="IV142_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAP8486364.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          541 a          319 c          291 g          669 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcggatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgccaccagt tgtcagaaga
     1381 caaaaacttt tagaccagaa catttctttc caaagaaatt gtttgactct tggaaagtca
     1441 cgttttatca atcttgagct ggcactttta taggcattga tgaattttgt caattctgtt
     1501 ttaggttgag ctttgaacat aatatgaacg tggtctttat catgattcca ctccactaaa
     1561 gtaatgtgat acgattctga aattctttca aaagtagttt tggcataatc agatatttca
     1621 tcatcaatca cttgtctacg atatttcgtt actaaaacaa ggtggtaata aagaagaaat
     1681 actgaatgat tatttgtatc taattccatg ttatatcaac tcatttcttg tatagactga
     1741 ttgtaacatg gaacaaaata aaaaggcaat acgccttagt tgtcggtctt cgagttacca
     1801 acggtaaccc tcataccgaa
//
LOCUS       DACDEG010000193         1752 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.201, whole genome shotgun sequence.
ACCESSION   DACDEG010000193 DACDEG010000000
VERSION     DACDEG010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.201"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            383..1633
                     /locus_tag="IV142_002791"
     CDS             383..1633
                     /locus_tag="IV142_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8486365.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNNSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQH
                     FLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGK
                     GRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQ
                     VIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQ
                     SQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARK
                     FTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          578 a          335 c          309 g          530 t
ORIGIN      
        1 ttgtctaata gtttttattc aaaatgtttc tagattataa aatttattga ataacacatt
       61 cctaacggaa tttgtttcga tattgtgtga tttctcagag tacaatggat gtatggaaag
      121 gatccaacgg gaatttgcta tttcctcctg tagcgtttgt ctaccctgtt tagtctgtta
      181 cccgaaccac tgttatcact accaactcat tagctgtttc gataatggac tagactttca
      241 aatagtagtt tggaaggcag cttctagctc ttttatcacc gtattctatt aggcgaggtt
      301 gtggctatac agtgagtttc tgggaaatcc cacagtatcg aagcattttc caaaatttat
      361 atcgaaagga ggtccttctt ccatgaaatt atttgtcggt attgacgtta gctctgaaaa
      421 actagatgtt tgttttttaa cagacgacaa tcaattgtct atcttatctg aaatctctgt
      481 tgccaatgac atcgaaggtg cttcattcat acgagaaaca attcttgaat tcaataacag
      541 ctaccacttt gatcaaattg tgattggcat ggagtcaacg tccatgtaca gcttccatcc
      601 ttccatgttt tttcatgaag atgagaaact aaagaagctc aacacacttg taacgattga
      661 aaatcctttt cgggtgaaac aatttagtcg catgtttgat gagaataaaa ctgatcgaaa
      721 cgatgcatta agaatcgctg atttcttgcg tattcaacga ttcacaactt cccctatcaa
      781 agaagagaaa tacatggcct tacagcgttt aacacgtact cgctatcaac ttattaagca
      841 attgattcgt acaaagcaac attttcttga aaatctaacg tacaaatgta atacgcttgc
      901 tcgagaaatg cgagacgaat cgacgagtct ttttagtgca accctcattt cactcatgac
      961 ggaagatttc acattggatg aacttgctga gcttccttta gaagctttct gtgatttact
     1021 tcaagaaaaa ggaaaaggcc gtttcaagca accagaaaaa atcgctaaag caattcaacg
     1081 tgcaattacc atgagttacc gcctaggtgc tcttgcacaa gaatcaatta atgttgtttt
     1141 aagtgttctc gtacgagaaa tacgagcact tgaaaaaaat atcaaagaat tagataaagc
     1201 catcgaacaa gtaattgttg tcattccaga atatcaatgc ttaaccagta ttccaggcgt
     1261 tggtaaagtc tatgctgccg gtttaatcgc tgaaattggt caaatcgaaa gatttgaaga
     1321 tcagacaaaa ttggcaaaat acgctggatt aagttggaaa gtgaaccaat ctggaaacta
     1381 tcagtcgcaa aatacacctc tgacaaaaca aggaaatcga tattttagat actacttagt
     1441 tgaagctgcc aactctgtaa aaaattatct tcctgaatac aaagcttttt atcaaagtaa
     1501 atataaggag gttccaaagc atcaacacaa acgtgcactc gtcctaaccg caagaaaatt
     1561 tacgcgtctg gtggatacgc tactacgtaa acaccaactc tatacgccac caaggagtgt
     1621 gatagagaaa taacaatccg ttattgacgc aaatccttga atcaacccga aaaaaatttg
     1681 attttgatcg ggtctagttt cgtgcgcttc aaaacatatt actcaataaa gaaaatctta
     1741 aatttcatct tg
//
LOCUS       DACDEG010000194         1744 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.202, whole genome shotgun sequence.
ACCESSION   DACDEG010000194 DACDEG010000000
VERSION     DACDEG010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.202"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1258
                     /locus_tag="IV142_002792"
     CDS             <1..1258
                     /locus_tag="IV142_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486366.1"
                     /translation="IAEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVL
                     TTLLCSALIACMIIVRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNY
                     QEIHSPFKTDLQLEALSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEK
                     IIYVGVVSTWVCVTNLFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMN
                     KKYSWLIIGATVVFLLVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDY
                     APTEAIKERPHLEKHEVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQ
                     VKVNGKVVNDSYKKHGLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSF
                     KRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT          614 a          251 c          296 g          583 t
ORIGIN      
        1 aattgcagaa gcaatcgctc ttacgattat cccagctgtt ttatattatg cttatggatt
       61 gatattcaat agagaaaaaa aagtcatcgc tttatctgtc agtataagtc tacttgtgta
      121 tactcatgtc ttaactacac tattgtgtag tgcattgatt gcttgtatga ttatagttcg
      181 tttgttttta agcaagcaaa taaaaaacga tataaaattt gtggtcgtga atttagtaaa
      241 ggctgttttt ttgacttgtg cattaactag ctatttttgg tatcctatgt tgcagcagat
      301 gaattatcaa gaaatacaca gtcccttcaa aacagatttg cagctagaag cactttctat
      361 ttcagatagt atcattcaag cgttaaataa cgatattaca acatacacta tggggttatt
      421 aggattggtt gcgttgattt ttcctttatt catactgaaa gaaatggaga aaaaagaaaa
      481 aatcatctat gttggcgtag tttctacatg ggtatgcgta actaatttat ttccatggtt
      541 tttgtttcaa aaaacaccga taagcttact ccagtttcct tggcgtatct taggtatcca
      601 aatctttttt tcttcaatga tcataacgct tgtttttatg aaaaagacta tgaataaaaa
      661 atattcatgg ttaatcattg gcgctactgt ggtgtttcta ctcgtcacgt ctatagcagc
      721 aaaagaaaat tacgcaaaag ttatccaatc aaaacacggt catattgtta tcaatgaaga
      781 aacactccca cgttatacaa catcagatat gggaggatta tacgattacg ccccaacaga
      841 agcgataaaa gaaaggcctc acttagaaaa acatgaagta aaagtaggag aatcgtggga
      901 aaaagggaag tataaagctg cagataataa cattacttac acagtggcaa gcaacaaaaa
      961 gcaaaaagtg acagtaccag tatatgctta tttaggtaca caggtaaaag taaacggtaa
     1021 agtcgtaaat gactcttata aaaaacatgg cttagtgaat gtggatgtaa atgctggaga
     1081 aaataagata gaaattacaa caaaatatac tccaactgct ctcatggctt tatggatcag
     1141 tattctgact tattgtttag tggtgtattc aagttttaaa cgatttgcac ctaataagtt
     1201 gaaagattct cgatatgtga ctaaaaagga gataaacaag aagaatgaaa ataaataaaa
     1261 aagtagtaat attgagtgga ttaattatag ttagtagtat atattctttc atctttttca
     1321 aaaatacaca gagtatttat accactggag atctttcttt tcacttatct cgaatcaagg
     1381 ggttatcgac tatttttacc agtccgatta attatgagac attcaattat actggatatg
     1441 gtgtaaatta tttttatccc tttctgactt tttttccagc tgtaatgtta tattggatga
     1501 caaaaaattt gattgtaagc tatattattt acgtttggtt attgaacttg tgtacaacac
     1561 tcgtagctta tcattatggc gagcggtttt taaaacaaaa aaaagcggca tttttatttt
     1621 aagtagttca gttttggagt acaaaatgac aaaatgtact tcaaaatgga ctactttttt
     1681 cattttaact tacataccaa attacataca aaatgattta taaaatgcaa ttgaaaatga
     1741 taat
//
LOCUS       DACDEG010000195         1716 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.203, whole genome shotgun sequence.
ACCESSION   DACDEG010000195 DACDEG010000000
VERSION     DACDEG010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.203"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..556
                     /locus_tag="IV142_002793"
     CDS             <1..556
                     /locus_tag="IV142_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8486367.1"
                     /translation="REYNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLL
                     LFLWMNARKKEIAVLLSLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNN
                     ILNKVTGDIAKQIAKQSASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVL
                     LVSLIISSFNILRRNPKELLIDNN"
     gene            complement(842..>1716)
                     /locus_tag="IV142_002794"
     CDS             complement(842..>1716)
                     /locus_tag="IV142_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8486368.1"
                     /translation="DSQVAFQLVKQSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRP
                     CLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTRERYREYKSNPKECVN
                     CPLLNQCTKSKNHQRVITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKE
                     KHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQ
                     ARVTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNINVYFTKARFKRLIAITVSS
                     IVMFLWLFRMGVIP"
BASE COUNT          549 a          294 c          312 g          561 t
ORIGIN      
        1 gagagaatat aatttgatta aaagttcatc aaattaccct gcattacaac aatctatatc
       61 aggtatttac tcaatttcaa ataaattatt ttttggctca ttaatatttg caggagttgt
      121 agtttcatta ttattattct tatggatgaa tgctagaaag aaagaaatag cagtattact
      181 atcattaggt atatcaaaat tagaaatttt tggtcaattc ctaattgaga tggtatttat
      241 atcaatcccc gcattcgttg gttcctattt cttagctcaa tatacagctg acaagcttgg
      301 gaataatata ttgaataagg ttactggtga tatagctaaa caaatagcta aacaatctgc
      361 atctagccaa ttaggtggtg gggcagaagc tgaaggattt aacaagacat tatcaagttt
      421 agatatcaat gtattaccta aattcatgat ttatgtagtt atatttatga gtttagtact
      481 tcttgtatca ttgattattt cttcattcaa tatattaaga aggaatccaa aagaattatt
      541 aattgacaat aattaaaatt ttcggtgctt tttagcacca aaaatgagag tgtaatataa
      601 accgtgtaag taaccatcaa tcctaggcgg gtggttgctt cacggttttt atttttctaa
      661 aataagatca agaggtgatt acacgatggc tagaaagaaa agaaatcctg atgctgagaa
      721 gttagctgaa tccattttga atacttatca acccgaatct gtagaggata tgcaagatgt
      781 tttaaaagat gtgtttggtc ccttatttga aaaaatgctt caaggagaat tgaataacca
      841 tttagggtat gacgcccatt ctaaagagcc aaagaaacat gacaatcgac gaaacggtta
      901 tggcaataag acgcttaaaa cgagctttgg taaagtaaac attgatgttc ccagagaccg
      961 ggaggcttct tttgaacctg agttgattcc taatagaaaa cgagatgtct ccgacatcgc
     1021 agactgtaga caaaagctgt tccaaactcg taactcgagc ttggaacagc tttttatcta
     1081 cttcgtttcg aatttttgaa caaaaaaata aaggctcctt gcctttccat agccaggtgg
     1141 ctaatttctt taaattcatt gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca
     1201 agcctcgata cttggtccaa cgcatcccat gcttttcttt tgcatccgca aatattcgtt
     1261 caatcgtttg tttccgtttt ttgtaaattg atttattcag atctgttagg cgaagatgtt
     1321 ccacttcatc catcagatct ccccaaacat gtctcgtgat gactcgttga tgatttttac
     1381 ttttggtaca ttggtttagc agaggacagt tcacacattc ttttggattg gatttgtatt
     1441 ctcggtacct ttctcgagta accgttgaaa aagcgagcat ttgatcttgc ggacaaatat
     1501 aacaatcaaa atactcgtca tagagaaact cattttttga taatagcccc tttttccctt
     1561 tgggccttga ataaggcaaa caaggtcgaa ggttcaaatg agtcaaaaag tgaacaaact
     1621 taggtgtctt atacccagca tcagcgacaa cacaattgat ttcaggaaaa agtctcttcg
     1681 attgtttgac gagctgaaag gcgacttgac tgtcat
//
LOCUS       DACDEG010000196         1710 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.204, whole genome shotgun sequence.
ACCESSION   DACDEG010000196 DACDEG010000000
VERSION     DACDEG010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.204"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            474..1073
                     /locus_tag="IV142_002795"
     CDS             474..1073
                     /locus_tag="IV142_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8486369.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            1137..>1710
                     /locus_tag="IV142_002796"
     CDS             1137..>1710
                     /locus_tag="IV142_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486370.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIGD"
BASE COUNT          615 a          260 c          322 g          513 t
ORIGIN      
        1 aaccattgac acaaaattat tgacgctccc aactaaaagt gtcaaaataa tgatgaaatc
       61 atttagttct tataaattag aaaagagaaa ataaatattc catatctact ttattggatc
      121 cgtaacatga ctaaattctg aatacttttt aaagcttatc atttggtaaa actgaatata
      181 agttaattat atagactgaa aattttgaat tttcagaata gaataactta aaagtactga
      241 caaaattttt taaaggggca atattaatgt tctgagataa tcaaatccta ccgaaaatat
      301 caaaaatcca atgtgacaca atcagattgt gtctcattcg agggaatcac acaatcccac
      361 gtggctctag gcagaattga atttttgctt ggggcttttt ccttatattg attttgaatg
      421 agacttatta tatgttataa tgcaattaag tatcattgga gagggcgaag aaaatgaaga
      481 cggtagaacc tattcgagac aaaaaaaaga tcgatgcgat gaaggcaatc ttggcttcgg
      541 gaaaatatgg ccaacgaaat ttggttcttt tttcgattgg gatcaacacc gcctatcgca
      601 tttctgattt gcgtcaattg aagctggccg acgtgctgga aatttcacgc ggtcgcgtgg
      661 tggtcaaaga tcgattagcg atgaaggaac aaaaaacagc taaacataat tcggtctata
      721 tttccaacaa attgcgaaaa acgattttgg agtacgttca acaggaattt cctgatcaac
      781 tccttcagca agattttgac ggttatttgt tccctagtcg aaaagggacg aatgaaccgc
      841 tcaaccgcca aagtctgtgg cgaatcattc atgatgctgg cacggcaatc ggattgaaag
      901 acattggccc acattccatg cgaaaaacgt tcggttactt tctctataaa caggggacca
      961 aaacagaaat cattcagtcc ttgttgaacc attcttctca gcgcgaaaca ttgcggtata
     1021 ttggtattac acaagaagac aaagatacag ctgtaaaaag cttagattta taatataaaa
     1081 gttaagttta aacatggata gttttaaatt agtagattga gtgttgggag gaaagaatga
     1141 gtacattata tattatagga aacggttttg atttaagtca tggtttaaaa actagataca
     1201 ctgatttttc aaaatgggtt gaaaaaagtc atagtgaaat attcgataaa atagacacaa
     1261 tgataaatga aaataatgag gtgagaagta cagctgatga taagataggt tggtttaatt
     1321 ttgaaagtag tttgcatgag ttaattcaaa gtccagtctt tgaagattat gtagaagagt
     1381 ctaaggatta tctcataggt tatggacatg aagattggtc agattctgcc catcatgatt
     1441 ttcaaaatat ggtttatgat tatgttgttt ctctagaaga attacaacaa cttttcaagg
     1501 aatggatacg caaagaaata cagccgacaa tagaacataa aattccaaaa taccaatttt
     1561 cgtccgatgt actattcttt acattcaatt atacagatgt tttggaaatt ctatacaagc
     1621 aaccgaaaga aaacatatat catattcatg gcagttatga cgatataata atcggccact
     1681 ccgagaattt tgacaacttt ataggagatc
//
LOCUS       DACDEG010000197         1706 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.205, whole genome shotgun sequence.
ACCESSION   DACDEG010000197 DACDEG010000000
VERSION     DACDEG010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.205"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(150..440)
                     /locus_tag="IV142_002797"
     CDS             complement(150..440)
                     /locus_tag="IV142_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP8486371.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(576..755)
                     /locus_tag="IV142_002798"
     CDS             complement(576..755)
                     /locus_tag="IV142_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486372.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(752..1102)
                     /locus_tag="IV142_002799"
     CDS             complement(752..1102)
                     /locus_tag="IV142_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486373.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(1095..1601)
                     /locus_tag="IV142_002800"
                     /pseudo
     CDS             complement(1095..1601)
                     /locus_tag="IV142_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
BASE COUNT          526 a          320 c          258 g          602 t
ORIGIN      
        1 gcgagcagca actagaataa tttaaacatt attaaactgt ctatattgtt actaataatt
       61 cccttcttat agtgcatttt cttagaaaaa aacaatatta cgatatcatg atattaagat
      121 atcgtaatat tatacaattg tgattgatat tatatatgtt tttttatttt tttatcatgt
      181 ttaatcttga aggaatcata ttctttttct aaagacagga gcttttcata aacatcagac
      241 atgtttaaac tagaggccag gcttaaatca taaacgaatg aagcaggtaa tgattttatt
      301 ctacgttgtc gggttatcca gcttgcaacc gttccttgtg ggtaggcata taactcacaa
      361 aattgttcta cagtgattcc taaattcttg ataatgtaaa cattgatcgg gtgcgagtaa
      421 acaaatgtat tgatagacat ggtgcgtaag cctactttct tttattattg cgtttgcaat
      481 aatagtttaa cacacgcatt attctagtat ctactcctat ttgtaaaaac aaaaaaggat
      541 tcatagagat aactctatag aatccgtaaa ataccttata tatataacgg gaaaatataa
      601 aacctaaaat tatgataacc tgctgcagct tctacataga aagagaagcg ctcaacacca
      661 acctcattta tcaaacgttg gccataaccc caaatctctt ctgatagctg atcaaaggtt
      721 atatacttta gctcatattg ctggataatg ttcattctaa tcgactccat ttattatcct
      781 ggtaaaattc gatatgacgt atttcgtcgt agtgaatttt gtcttttcca acgtagatcc
      841 ccagctcatc actacctaag atcttgccaa caatatcttc tggatagtat ccttcagcgt
      901 ttcgttcttc tttttgaata gatactgatc tattttttat gactgcagcg ttcaatactt
      961 ccgcaatctc ttcagtcgtc atttgttctt ttgctaaatt ttgactggtt cgttgttcat
     1021 tgtcggcatc aatttcttca gtatgatcag ataaataaaa ccctgcccac tttaaacgtc
     1081 cacgatcatt gtaatcaatc atttttaatt cccccatttc cgcccgcatg accaccaaca
     1141 agtgtgcttc tagtaatgct tctagcgctt tctaacattg aagaagcatg tacaatcgac
     1201 gtaaaaccat atttttgccg aatcttgtca ataattttat ctaaattttc ttcgttaatt
     1261 tgcttttgtg gatcactaaa taaatccagc tgaagcgaat cggtgtacac aagcttgcta
     1321 taggttattc caacgtgcct tacttcttgt ccttcataat acttatcaaa caagaaaaga
     1381 caataattct gcaattcctt tgtattatct gtgataggta ttttcatctg gtgtgaaaat
     1441 cctggtctcg tttctaagat cgacgtacca atgtttaaat gaacacatcc tgttttacaa
     1501 ttatgctgcc tgatacgaat ggctacttgt tccgccattt cttttaccac cagctcgatt
     1561 tcatttcttc tagtataatc tcgtggtaac acttgtgaat tacggttctg ttgcaaagtt
     1621 ttaaatctac tatcagataa ggtagaataa tagaaaaaga tagcaggagg aatgacgatg
     1681 agtcatttta aaggaaagca atttca
//
LOCUS       DACDEG010000198         1686 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.206, whole genome shotgun sequence.
ACCESSION   DACDEG010000198 DACDEG010000000
VERSION     DACDEG010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.206"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            153..1631
                     /locus_tag="IV142_002801"
     CDS             153..1631
                     /locus_tag="IV142_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8486374.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          614 a          230 c          302 g          540 t
ORIGIN      
        1 attttttgca atattctcaa aaaaaagata aaaaatgaga ataactgatg aaaaaacaag
       61 cataatccat cataaaatca gtataggtga aaagatgaac tttcatctgc atatcaagta
      121 attggtatga ggcatttttt gaggtgtagg atatgaagtt ggattacctt atagaagagg
      181 aagatagaga aaaactgctg ctagctagat atttagaatt tgagaaaaag aattatgtag
      241 tgctgcctaa gttatatgaa acattaggtt tgtctaaatt taaaacaaga aattatatca
      301 tagaattaaa taaagaattt actgaattca aaacaaagcc agagatccaa atttcagaag
      361 aagcagagat ccatgtcgaa cgtctgaatt tgacggatat caaaaattta agaagaatct
      421 actttcaacg atcaaaaatt gctcgactgt taacggagat cgtggattca aaagtaacga
      481 tcgaaaaatt tgcagagaat cattttttaa gcatatcaag agcctatgta aaaagaagag
      541 aattagtctc ttttcttgaa cagcatcaaa taaagttaaa gaaaaacata ttagttgggg
      601 aagaaataaa catacggaac gtattatttg gggtatattt cggggtcttt aatggttttt
      661 ctatgccatt tagagaaagc gtcatgaatg aagcaaagag tatcgtgcaa tattttgaat
      721 atctgttcaa tttaaaactg tctgcaacaa aacgaatcaa attcaatttg ctaaccacta
      781 ttagtttgtt gagggcctat gggaatcaac caatcggaaa acgtttcttt tcagatgaat
      841 actttaagag ctcagaagga atgaagatca tcaaggattt atcacatatt tcttttcttc
      901 atatccctaa agagcagatc attgatgaga tttcttatat catgttgttt ttgatcacgg
      961 aagaagaatt tgaattagat ggcgcatttg gacttgcgga agctcatttt catgatatcc
     1021 acatgattag tcaaaaaatg tcagtggaat tgattgatca attaccattt tcgattgaaa
     1081 tctctgaaga agaaagacta tatatacgaa aagaacttcg tatgggtatg atgcatgtca
     1141 atcgaaaaaa ttggatattc gactttgaga tgactagctt tacgtctaaa aagcagctac
     1201 agtttttctt cgagacgtat ccaatattca gtacagcaat ccaaaaggtc atacaagaga
     1261 ataaacgcta tttcttggga aaagatgaac gtttcatgat tcgcttattt tatgattatc
     1321 tctttttatt ggtcggtatt tgtccggtat catattttga agcaccgatt catgtctgta
     1381 ttgatttttc gcaaggatct gcttataccg attacattat ttctcaagtg aaaggattta
     1441 aaaattacaa tattgtgatt gagagtcgaa tcacttctcg tacgaatatt tatttatctg
     1501 attgtttact tgaaaaattt aataaagaac agataatttg gaaaaatcca ccgactccag
     1561 atgactggga atattttgga aataccatca tcagagaaaa aaatagacat ttaactgaaa
     1621 aaatcagcta gtatttatgg atggcagctt tgtctcacat atttattagc tgatttatgt
     1681 tttttt
//
LOCUS       DACDEG010000199         1664 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.207, whole genome shotgun sequence.
ACCESSION   DACDEG010000199 DACDEG010000000
VERSION     DACDEG010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.207"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            131..505
                     /locus_tag="IV142_002802"
     CDS             131..505
                     /locus_tag="IV142_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8486375.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IV142_002803"
     CDS             complement(619..1248)
                     /locus_tag="IV142_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8486376.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IV142_002804"
     tRNA            complement(1382..1454)
                     /locus_tag="IV142_002804"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DACDEG010000200         1651 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.208, whole genome shotgun sequence.
ACCESSION   DACDEG010000200 DACDEG010000000
VERSION     DACDEG010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.208"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            337..927
                     /locus_tag="IV142_002805"
     CDS             337..927
                     /locus_tag="IV142_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HAP8486377.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            954..>1651
                     /locus_tag="IV142_002806"
     CDS             954..>1651
                     /locus_tag="IV142_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP8486378.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKV"
BASE COUNT          578 a          179 c          317 g          577 t
ORIGIN      
        1 tagaaatagg agattttaat cataaaaaac tgtactatag tgaatttttg gagatttata
       61 ttccagttat atattaatga ttgtgatact tgtactattg taaaaattcg ttaggtttaa
      121 ataactggaa atatcggaaa atgggcatta tacatcacgg acatcccttc ttttggaaaa
      181 aatgttacga tttttataag taatattgta tccggtggaa tcaacgatga agataacata
      241 tccatcgttg tagattcagt agcattgaat gatgctcttg ttgcaagaaa tccgaataaa
      301 attgttaagt ataaattgaa ttaataggag atatgaatgg aaattagcag taaagaatat
      361 ttaaaagcgg ttcttcgtat ggaaaagaat ctatatatag aatctaaagg acaacacaca
      421 aaattactaa aaataatgaa atcacatccg gactacaacg cttggcggta tactcgtcga
      481 atgagaatta catcttatta ttattcaaag agaaagaaaa atatcattta tgcgattatg
      541 tatattgtgt cttgcagaag aatgaatagt ttgggaagaa aatgtggtat agagtgtggt
      601 gaaaatgtat ttgatgaagg tgtaagattt taccatacac aaggaattgt tattaatggt
      661 aacgccataa taggaaaaaa ttgttactta tatgggaata actgtattgg aaatgatgga
      721 atcgatccta agtgtcctgt aattggaaat aatgtacgtg tatgcgtggg tgcaaagatt
      781 attggtaatg taaaaatagc taacaatgta gttgttgcgg caggagctat tgttatcaaa
      841 gattgtctag agaatggtgc aattcttgca ggtgtaccgg caaaaataat agggtatgtt
      901 aatttaacaa aagtattttt agaataattt ttagcgaaag aaggtgaata acgatgcgta
      961 tttcaataaa aagatggtca ggagcagatt ggacattgtt cacgttggca ttttttgcaa
     1021 tctccatagt tcttgtatat acttttaacg ccccaagagc aattttatat ttgggagata
     1081 taattaatat ctttgtgtta ttaaatgcac ttagctttag acgcagaaaa ggaatatatg
     1141 ctagcaaaaa tgagatgctg atagttttgt ttttactagt ttctatttgt agctcagtta
     1201 ttaatttaga aaatatagga ttgattatct gggggatacg aaataacact cgtatgttca
     1261 tagtatatat aaattgtgca acatatttaa caattaaaga ttactataaa gtattcgcta
     1321 taattgaaaa attattctgg atttcactgc cactttgttt aattgaaaga ttcatggtca
     1381 cctatccaac tggaacaatt gtaggagata tgataggtgg agttttttgg aattattcag
     1441 gttgtaatat gccattgaat ttaattttgt gtgtgacagt tactgttgtt acaatcggat
     1501 atttcaaggg agaaaaaaat ttcatccatt ttattttatg tgtttgtgca gcacttttta
     1561 tgtctgcatt agctgaattg aaagttttta ttcctgaatt atttataata tttgttgcaa
     1621 tagtattcac tttaaaagtt gattttaaag t
//
LOCUS       DACDEG010000201         1645 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.209, whole genome shotgun sequence.
ACCESSION   DACDEG010000201 DACDEG010000000
VERSION     DACDEG010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.209"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>1645)
                     /locus_tag="IV142_002807"
     CDS             complement(<1..>1645)
                     /locus_tag="IV142_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAP8486379.1"
                     /translation="HLDEDAISWIEETIDAMTLEEKIGQLFVNMGSSRDKNYLSSMVN
                     QYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGACTDGTEVGVEVKI
                     GATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISSRSFGQSPDKVLE
                     LSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWDNSFGKVYSGLIE
                     AGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGKLGFNGLVVTDAS
                     HMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKGIISEERLRDALR
                     RILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQAITLVKDKQDIWP
                     IVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFEVEKYISPEEELK
                     GLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIVWPASKGTPDIPF
                     YIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEI"
BASE COUNT          514 a          330 c          211 g          590 t
ORIGIN      
        1 tatttctaaa gtttcttgtt ttccatcata agtattgata taagttttta cttgaggcac
       61 atcagctaaa tgaaacggac actgtacaga tacaaaaatt gttggaattt catgaatata
      121 aaatggaata tccggagtac ctttacttgc tggccaaaca attctttgaa ctgtacctgg
      181 gttaacatta gctatattaa taatcaagtc atatttttca gttaaatctt tgattggtct
      241 cttagcagca tatattccag ctatattttt aattttttct tcttgcgata atcctttcaa
      301 ttcttcttca ggtgaaatgt atttttcaac ttcaaatcct tttgctgaca aaatctgttt
      361 caataaatca atggcctgag gtctatttcc agcaactact tcaccaaacc caccttttac
      421 cccatgtaca tctaccagta aaatcctttt atatttttct ggtacaatag gccaaatatc
      481 ttgtttatct ttaactagtg taattgcttg atcagctacc tctttaaaaa ttgatttatt
      541 ttcaattaaa ccaatttttt ccatagcttc ttgtttcggt aacaaaattt tattcttttc
      601 ttttttatgc aaacctagtt tagcctttaa gcctaaaatt cttcttaatg catcacgtaa
      661 acgctcttct gaaataattc ctttgtaata accttccatc atataattaa agtcttcatc
      721 tggatcatta aaaaatagga atagatcaca tccagccgca atagaagtgg gcaataattc
      781 tgaccgtttc attgccccag tcattcctaa catatgacta gcatcagtaa ctactaatcc
      841 attaaacccg agttttcctc taagtaagtc agtgattaat tcttttgata aagttgcagg
      901 aagtaaatct tcatctttca gatcaagatt aaaatgtttt gaataagctg gcatatggat
      961 atgacctgcc ataattgaag gtaatccagc ttctattaag cctgagtata cttttccaaa
     1021 agaattatcc cattcttctg tacttaaact attaatacta aaagatagat gttgatctcg
     1081 ttcgtcaata ccgtcaccag ggaaatgttt agctgcaggc aaaattccac tctcttgaat
     1141 acctttcata taagctaaac taagttctaa aactttatca gggctttgtc caaaagatcg
     1201 actagaaatg ataggatttc tccaatttct attaatatct acaataggag caaaactcca
     1261 gttacatcca atagccgatg cttcaattcc agaaactctc cctaattcat aagcatattt
     1321 cgcatcattt gttgcaccaa tttttacttc tacaccaact tcggtaccat ctgtacaagc
     1381 tccgttacca cctgcttctg tattagcagc aattaataaa ggaattttgc tttctgtttg
     1441 taggatatag ttttgatcat atacctcatc agcagttcct ggattatatc gtacagctcc
     1501 aatatggtac tgattgacca tactacttaa gtaattttta tcacgactag atcccatatt
     1561 aacaaataat tgacctattt tttcttctaa agtcatagca tcaatcgtct cttcaatcca
     1621 agaaatagca tcttcatcta aatga
//
LOCUS       DACDEG010000202         1612 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.210, whole genome shotgun sequence.
ACCESSION   DACDEG010000202 DACDEG010000000
VERSION     DACDEG010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.210"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            86..943
                     /locus_tag="IV142_002808"
     CDS             86..943
                     /locus_tag="IV142_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8486380.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            944..1492
                     /locus_tag="IV142_002809"
     CDS             944..1492
                     /locus_tag="IV142_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486381.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
BASE COUNT          616 a          223 c          336 g          437 t
ORIGIN      
        1 atatatcttt tttttggtag taactaaaaa taaatatgtt ataatagtat caaatgaata
       61 ttgctaaaag aaaagaggta ggaacgtggt agcgaagaaa tatgtaatcg gtaattttaa
      121 aggtggcgta ggtaaaagta cgtgcgcgca aatgttaggg tttgaatcag cagttaatga
      181 aggaaaaaga accttgataa ttgacttgga tatgcaagga aatacttctg atgtgatgaa
      241 cttaacgcat atgaacttca ctaacgaaga aggaggcgga gaaggtgaac cgattgttta
      301 cgaaaatact gtgactgacg tgttgataaa caatcttcca cctaaagagg ctgtctataa
      361 agtaatcaat aacctctata tacttccagc agacatgtcc tttgaaatgt atgatgattg
      421 gatcaaagac caatataatg attcattatc ccaattcaaa tatatgaaag aaaagctaga
      481 accattattt gatgagtttg acgtcatcta tttagatgtc ccaccttcta tttctgtata
      541 tagtaaatcc gcgatgtaca ttgcggattg ggcaatcgtc gtcttacaga cccaagtaaa
      601 atcgatgaga aatgcgatgc aatatcttga gtatatggac ttttttgtga aagaatttga
      661 tacaaattta tatgttgctg gtgttattcc tttcatgttg gaaagcggtg acgcagtaga
      721 tcaggaaatg tataaacaag cccaagagat ttatggggaa catttgatta aaaatgttgt
      781 actcaaaaat gcgcgcttaa aacgatacga cggatcaggt attacgatgg agaagacaaa
      841 gaaaggtctc ttaaaacaat gggatcgcaa aagccacgaa ttatttatca gtattttgaa
      901 agaattggaa gaacacgaga attggtatgc ggaggtggag taattggctg acatcaaagg
      961 acttgatatt tttgggaaca acaaaggact cgccaaaata aaaaaagaag cagaagcaaa
     1021 gaaaaagcag caagcaatta caccatctga aaataagcaa ttagaagtgc accaagttca
     1081 agaaataaaa acttcctcca atgttgaaag acagagtaga ggaagaacaa aaaaagttgg
     1141 cgcaccaatc agaaagtttg ataaggttta ttccaccaag catcctttaa aactgtcgac
     1201 tttgttaaat gcgacttcca gagttttagt agaaaagtat gagactaata tgacaagaga
     1261 tgaattgctt agaaaagctt tagatgagta tattaaacag aatcttacgc aagaagataa
     1321 acaagatttg tttaatgacg tggtgaaaga actagacttg ttcagagaaa aatatccaat
     1381 tgttccagaa ttagatgatc gtggagaaat aataagaact accagtcaga taaaacaaga
     1441 taccgaagac tcactgcgca aagggtgggg aatgaataat aaagttagat aataatcaaa
     1501 gaagaactaa gtaactacct ttaaacggta gttacttagt tcttctttga tttgcgtgaa
     1561 atagagggga tttatttttc tggttacgtg attaaaaaat ccaaaaaatg ag
//
LOCUS       DACDEG010000203         1611 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.211, whole genome shotgun sequence.
ACCESSION   DACDEG010000203 DACDEG010000000
VERSION     DACDEG010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.211"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>1611)
                     /locus_tag="IV142_002810"
     rRNA            complement(<1..>1611)
                     /locus_tag="IV142_002810"
                     /product="23S ribosomal RNA"
BASE COUNT          341 a          500 c          350 g          420 t
ORIGIN      
        1 aatgcccttg gcagaacaac tggtacacca gcggtaagtc catcccggtc ctctcgtact
       61 aaggacagct cctctcaaat ttccaacgcc cgcgacggat agggaccgaa ctgtctcacg
      121 acgttctgaa cccagctcgc gtgccgcttt aatgggcgaa cagcccaacc cttgggaccg
      181 actacagccc caggatgcga cgagccgaca tcgaggtgcc aaacctcccc gtcgatgtgg
      241 actcttgggg gagataagcc tgttatcccc agggtagctt ttatccgttg agcgatggcc
      301 cttccatgcg gaaccaccgg atcactaagc ccgactttcg tccctgctcg acttgttggt
      361 ctcgcagtca agctcccttc tgcctttaca ctcttcgaat gatttccaac cattctgagg
      421 gaacctttgg gcgcctccgt taccttttag gaggcgaccg ccccagtcaa actgcccatc
      481 tgacactgtc tcccaccacg attagtggtg cgggttagag tggccataac gcaggggtag
      541 tatcccacca gcgcctccat cgaaactagc gttccgattt ctacggctcc tacctatcct
      601 gtacatgcgg tacagacact caatatcaaa ctacagtaaa gctccatggg gtctttccgt
      661 cctgtcgcgg gtaacctgca tcttcacagg tactaaaatt tcaccgagtc tctcgttgag
      721 acagtgccca aatcgttacg cctttcgtgc gggtcggaac ttacccgaca aggaatttcg
      781 ctaccttagg accgttatag ttacggccgc cgtttactgg ggcttcaatt cgtaccttcg
      841 cttgcgctaa gcactcctct taaccttcca gcaccgggca ggcgtcagcc cctatacttc
      901 atcttacgat tttgcagaga cctgtgtttt tgataaacag tcgcttgggc ctattcactg
      961 cggctgatcg tatgatcagc accccttctc ccgaagttac ggggtcattt tgccgagttc
     1021 cttaacgaga gttctctcgc tcaccttagg attctctcct cgactacctg tgtcggtttg
     1081 cggtacgggc cgctgttttc tcactagaag cttttctcgg cagtgtgaca tcaggaactt
     1141 cggtactatt atttccctcc ccatcacagc ttgtccttaa gaaaagaagc atttgactcc
     1201 tcttcagact tactgcttgg acagacattt ccgatcgtct gcattcctta gcctcctgcg
     1261 tccctccatt gctcaaacaa aaacaacggg tacaggaata tcaacctgtt atccatcgcc
     1321 tacgcctgtc ggcctcggct taggtcccga ctaaccctgg gcggacgagc cttccccagg
     1381 aaaccttagt cattcggtgg acaggattct cacctgtctt tcgctactca taccggcatt
     1441 ctcacttcta agcgctccag cagtcctcac gatctgcctt caacgccctt agaacgctct
     1501 cctaccaata cacctaaagg tgtactccac agcttcggta atatgtttag ccccggtaca
     1561 ttttcggcgc agggtcactc gactagtgag ctattacgca ctctttaaat g
//
LOCUS       DACDEG010000204         1579 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.212, whole genome shotgun sequence.
ACCESSION   DACDEG010000204 DACDEG010000000
VERSION     DACDEG010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.212"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1350
                     /locus_tag="IV142_002811"
     CDS             <1..1350
                     /locus_tag="IV142_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8486382.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
BASE COUNT          621 a          242 c          336 g          380 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     1441 aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat
     1501 tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa
     1561 ttatgaagtt attctaatc
//
LOCUS       DACDEG010000205         1535 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.213, whole genome shotgun sequence.
ACCESSION   DACDEG010000205 DACDEG010000000
VERSION     DACDEG010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.213"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..490)
                     /locus_tag="IV142_002812"
     CDS             complement(<1..490)
                     /locus_tag="IV142_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8486383.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNS"
     gene            complement(492..1160)
                     /locus_tag="IV142_002813"
     CDS             complement(492..1160)
                     /locus_tag="IV142_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP8486384.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1290..>1535)
                     /locus_tag="IV142_002814"
     CDS             complement(1290..>1535)
                     /locus_tag="IV142_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8486385.1"
                     /translation="IVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSF
                     YATFYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
BASE COUNT          559 a          258 c          204 g          514 t
ORIGIN      
        1 tagagtttag cgataaacta catccaatag aaattttctg ccgttcacca cttgatagcg
       61 aaaaaatgtt tttatctaat atatttgaaa gatctaataa ttcagctaat tcattgattc
      121 ttttatccat atgctctttt gtaaatccaa agttttccat cgaaaatact aactcactcg
      181 ttgtatttgt tgtaaagaat tgtcctctag gatcttgact tacgtagcca atattttttg
      241 atatactttc ttgtgttaag gattgtagcg agttattttc aaataaaatt tccccaacca
      301 attttccgtc ataaagtctt ggaataagtc cattcattaa ctttaatagt gtggatttcc
      361 cactcccaga agaaccagta atcaatgtaa gactgccttt ttttattgaa aaattaaggt
      421 caacaagcgc ttgttcctta acatccttat atgaaaaaga aacattgtta aattcaaaca
      481 attctttcac ctcaaatcaa aatacttaag aaaaaaatag taacggaaaa caggatgata
      541 tagtctaata agctcatgtt tgttttatag taagaacttc ttttcacagg agattctatc
      601 ccctttgtta ttgcattagc ggtcatttct tcagcaatca tagttgcacg tataagaacg
      661 ggaactaaca gaaattccaa atattgcaga ggctgaaaaa aagacaagcg aatgtttctc
      721 atttttaaag attctttaat cattcttatt tcagaacgat aagtcggaaa gaatctgatc
      781 actactgtaa tacttaacat tatttttttc ggtaaatgta ttttttctaa agaggataat
      841 aattcattgc tagtatagac actaaccaac aaccatgaga tcattacgac cggcaacatt
      901 cgaaatacaa tatataagga tgtcgcaaaa aatatagtaa aaaactctat cttgatagca
      961 tcaataattt tcattactaa aaacagaact gaataaatca agatgaaata aatacatgtc
     1021 tttattttac gaaacatcaa tactaaaact gtagcaataa tgaaacaccc cacttgaaat
     1081 atatttccct ttcctaaaaa aataatgacc ggaaaaaaaa tatttaaagt aatcaaggtt
     1141 aacggattga tttttgacac tctttcacct ccaaaaaata agatcgcact gttatcttaa
     1201 gctgataaaa atgcaccgtt attcagataa cagtgcgatc ttatcaaaaa gcataactga
     1261 gaacaattct caatgcaagc tttttttaat taactcagta attcaatcca cccaggaaca
     1321 gaaaattgat acaatcttct ggttaatagc atagtctttt cttttgaata gctatgcttc
     1381 aatatatcat agaatgtagc gtagaatgag taaatgtaga agtggatttc ttcttcatta
     1441 aagactaaat tttgattgat cattccgata gtctttaact tatctagaca acagactgtc
     1501 tttttcgtca caaatgatgt aattttttca acaat
//
LOCUS       DACDEG010000206         1534 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.214, whole genome shotgun sequence.
ACCESSION   DACDEG010000206 DACDEG010000000
VERSION     DACDEG010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.214"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..943
                     /locus_tag="IV142_002815"
     CDS             <1..943
                     /locus_tag="IV142_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP8486386.1"
                     /translation="CKHCYNASGGDNCFMDNELTDDELLELTKDICNLKPYNICFCGG
                     ETLLRKKIVLSCATLLKEAGIPNIAIVTNGSLVTQDVAKELFKAGINRFQVSLDGKNA
                     SSHEALRLYPNSFEKAINAIKLFRELSEEVDVAFCPTSFNYNQLREVYQLVNTLKVKS
                     LRIQPFMLSGRGMQNREIVPTVSQYRKLVSDINYIKRNEDYTKIDWGDPIDHIIRHRT
                     LLKDFSNFVSIRSDGGLMASPYLPIQVGNIKCHSLSKYWRAGLYKVFSLDLVQDIATQ
                     VSSIQDMSKELKKGHIKWLNEDIEIDILDEYKHDILI"
     gene            999..1217
                     /locus_tag="IV142_002816"
     CDS             999..1217
                     /locus_tag="IV142_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486387.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            1288..>1534
                     /locus_tag="IV142_002817"
     CDS             1288..>1534
                     /locus_tag="IV142_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486388.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVV"
BASE COUNT          574 a          182 c          294 g          484 t
ORIGIN      
        1 atgtaaacat tgttacaatg cgagtggtgg agacaattgt tttatggata atgagttgac
       61 tgatgatgaa ctgttagaac ttacaaaaga tatatgtaat ttaaagccat ataatatttg
      121 tttctgtgga ggagaaactt tactgagaaa aaaaatagtt ttatcttgtg ccactttatt
      181 aaaagaggca ggtattccta atattgctat tgttactaat ggatcattag taactcagga
      241 tgttgcaaaa gaattattca aagcaggtat taatcgattt caagtaagtt tagatggaaa
      301 aaatgctagt agccatgaag cacttagatt atatccaaac agttttgaaa aggcaattaa
      361 tgctatcaaa ttatttagag aattgtccga agaagtggat gttgcttttt gtcctactag
      421 ttttaattac aatcaattac gagaagtata tcaactagta aatactctta aagtgaagtc
      481 tttaagaatt caaccattta tgcttagtgg taggggaatg caaaataggg aaatagtgcc
      541 aactgtttct caatatagaa aactagtttc tgatattaac tatattaaaa gaaatgaaga
      601 ttataccaag atagattggg gagatccaat tgatcatatc atccgacaca gaactttatt
      661 gaaagatttt tcgaattttg tttctattag gtcagacgga ggattaatgg catcacctta
      721 cttgccaatt caagttggaa atattaaatg tcacagtctt tctaaatatt ggcgagcagg
      781 actttataaa gttttttctt tagatttagt tcaagatata gctactcaag tttcttcaat
      841 acaagatatg agtaaagaat taaaaaaagg acatattaaa tggcttaatg aagatataga
      901 aattgatatt ctcgatgaat acaaacatga tattttaatt taggtttatc attggaaagt
      961 ttagataaac aaagtacttt tagaaaggag ggaaaaaaat gatttctgaa cctcaaatga
     1021 ataatatgta tgaaagccca actataatgg gagctggtga agctgcgcca agagcttgtt
     1081 cagcaattat ggtgtgtgtg ttattagcat ctgcaggagt aggtatccaa gcaggagcgg
     1141 tgtatactta tgctgtagtt gctacagtag ctgtagctgc agccgcagtt tggaaaacag
     1201 taggattagt tggttaggaa ttaataggag actaactgtc tcctattgat tccaatgatt
     1261 aagctaagga ggaattttaa gaaaataatg aaatacaata atttaaaacc gaaattactt
     1321 aatgagaaca tagaatatat aagagaagaa aattatggat atttaaccat agttcccaaa
     1381 aatggaactg aattgaaaga taacgttttc aatccaacag taagatatat attggctaac
     1441 tgtactggaa aaaacagtgt aaaaaaaata gcgaatggtt taaaaaagaa gtttcctagt
     1501 ttagaatatg aaaaattcta tcaagatgtt gtag
//
LOCUS       DACDEG010000207         1528 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.215, whole genome shotgun sequence.
ACCESSION   DACDEG010000207 DACDEG010000000
VERSION     DACDEG010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.215"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            266..1000
                     /locus_tag="IV142_002818"
     CDS             266..1000
                     /locus_tag="IV142_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486389.1"
                     /translation="MRSSKYTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVL
                     KGIEIGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFA
                     QLSEALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKS
                     AYLYDNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKAKGVTELAI
                     YQKGKRIMVPLQSKKTSSASKVRTFF"
     gene            complement(997..1494)
                     /locus_tag="IV142_002819"
     CDS             complement(997..1494)
                     /locus_tag="IV142_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8486390.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRIYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
BASE COUNT          496 a          261 c          243 g          528 t
ORIGIN      
        1 attgtttgct agatccaaac tcttattaaa ctttccttgc tataaaaccc aaacatagta
       61 ataaaagaat aacttattac ctttaaatta tactaccaac aaatggtagt atattattaa
      121 tttatataat atgaaaccta aaatatagta gtatacccgt ggtgctagtt aattttttcc
      181 tagaggaaaa tgtctaaaca tgttggtaaa tcaacaaaaa aacgcatata gtagtctcaa
      241 atctcccaag aaatgaggac tacatatgcg ttcatcaaaa tatactgaac attttgactt
      301 agccaatcct gttactaaag tcgatgatat ccccgattat gaaatgtaca gtcaaacaat
      361 cgatagctta aataagcgtt ttggcaatcg tgttttaaaa ggcatcgaaa tcggttacat
      421 cgcttctgaa aaggatcgga tcatcgacta tttagccgat aaagattacg atttaaaatt
      481 actcagtgtg catcataatg gtcaatttga ttatttagat gacgaagtca aagatatgga
      541 tcctgctatt gtgattcctc agtattttgc acaactttca gaagcactag ttgttataga
      601 agcagatgtc tttgctcatt ttgactatgg ttttcgggta tttggtctat ccgttgctga
      661 atttaaacaa tatgaagcgc agtttttacc gattctagat caagtaatca aaaataaatt
      721 ggcattcgaa ttaaatgcta agagcgctta cttgtatgac aatttagcgt tatatgaata
      781 tgtcatcgat ttatatcttt cacgtggtgg taccttgttt agtgttggat cagatgggca
      841 ttaccttgaa catttccgtt tccatttcga tgatcttttt gctctgttaa aagcaaaagg
      901 tgtgacggaa ttagcgattt atcaaaaagg aaagcgaatt atggttccat tacagtctaa
      961 aaaaacgtcc tcagcatcaa aagtgaggac gtttttttag atttcttttt ctaaaatcgg
     1021 acaaatacga tcttgttttc ctaaagggaa atgtttgatc ccgatttttt taaatccatg
     1081 acgttcataa aaacgaatac ctgcatgatt tttttcataa acagtcaaac gtaaataacg
     1141 tttttgtttc gttttagcat aacgattcgc ttcatcgagt aaccgttgcc ctaacccttt
     1201 gttttgataa tctttgagca gatagatccg ttgaatctct atagcatctt tttcatcatt
     1261 gactttcata taaccaatga gtatttgatg atccttaata ccaataaatt tactggttga
     1321 ctgtgccaat tctactgcca atatttcagt tgtcaattgt gtctgtaaat atccttgcat
     1381 atcaagtgga tctgcgccat cgataaacga ttcataaaaa ctgacaacac ttacaacttg
     1441 taaagcatct agatcatcat aatctaaata cgctagtgtg atttgattgt tcatataact
     1501 cctccttttg acatagtata ctataaat
//
LOCUS       DACDEG010000208         1507 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.216, whole genome shotgun sequence.
ACCESSION   DACDEG010000208 DACDEG010000000
VERSION     DACDEG010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.216"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..375
                     /locus_tag="IV142_002820"
     CDS             <1..375
                     /locus_tag="IV142_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486391.1"
                     /translation="SGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            362..>1507
                     /locus_tag="IV142_002821"
     CDS             362..>1507
                     /locus_tag="IV142_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP8486392.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPR"
BASE COUNT          485 a          200 c          241 g          581 t
ORIGIN      
        1 tctggaaaaa aagaaacttt aattaagctt catcctagag caaggacaaa tcgttatggt
       61 gaaaaaaaca tattgaattc cagtttaccg tttgaagtct tgctacttga aaatgacttc
      121 tcttcaacac ttttgattag tcctatatct acaatttctt tttcaccatc tatgatgttt
      181 gaaaagacga tcaactctat tatgttggct aaattaattt taaatgaata tacagcatta
      241 tctgaagata aaaaagaaac attgacaaat attgttcttt tctgtgaaca ttataaccgt
      301 ctatcagaag aaaaaatttt actcccagaa aactgggcag aattaagaaa gttggtagag
      361 tatgggatta tttaacacac tttccaagtc aaaattagtt aagtccagtt tctggtatac
      421 tatagggaat ttttttatta agggaatttc attcataact attcctattt ttgtaaatat
      481 tactactcaa gcagaatatg gtttgattaa taatttcact tcatttgtat caattttttc
      541 gattcttgtt gggctaagcc taaattcggc aattaataat gcaagttttg actataaaga
      601 tgacataaat gaatttatgt catcggtact ttctctagct acactttcgt taattgtgtt
      661 cttattaata gggaacatat attttttgtt ttcaaatgct ttttttgata tgaaccaact
      721 tgtatttaat ttgatgttat tacaaagttt tgctagtttt gtaattagtt atatccagtc
      781 ttattttacg attaacttta accattttaa atatttgatt ttatctttta tatctacttt
      841 tttgaatatt gcaatctctg tagctttgat gcagacatta tattttgatg ataaatatgt
      901 tggcagagta gtcggcggaa cactgtcatt tgtgttgata ggtttagtaa tttatttaag
      961 aataatgata aagggtgctc atttttttga taaaaaacag tggagatacg ctttaaaaat
     1021 ctctttgccg ctaatcccac attccttagc aaatatagtt ttagcacaat tcgatagaat
     1081 tatgattaat tcttatttag gtcctgctgc ggggggaata tattcctaca ttagtaacat
     1141 aggtattatt ctaagcgtaa tatggatgtc tactaataac gcctgggttc catggttcta
     1201 tggtgagatg agtaaaaata atgaaaaaaa agtgaagaaa gttagtaact attatttagt
     1261 agtctttact ttgattacga tgattgtaat ggtagtttcg attgatttag caaaatttat
     1321 ggctccgaaa gactattatt ctgggttatc tttagtggtt ccgataacct taggctattt
     1381 ctttcagttt ttatatagtt taccagttaa tgctgaattt tatgagaaaa aaacatcgtt
     1441 tattgcgatt ggtacagtag caagtgctat agttaatata ctattaaaca tgattttcat
     1501 acctagg
//
LOCUS       DACDEG010000209         1484 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.217, whole genome shotgun sequence.
ACCESSION   DACDEG010000209 DACDEG010000000
VERSION     DACDEG010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.217"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            151..1446
                     /locus_tag="IV142_002822"
     CDS             151..1446
                     /locus_tag="IV142_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8486393.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          465 a          293 c          278 g          448 t
ORIGIN      
        1 cctcaacgac gtttagttta tgaaaacagg ctctatactt tttgatgttg gtgaacttca
       61 ccaacatctt ttttgtataa aaaaatatag aaaagacacc ctgaaatcct ttagaattaa
      121 tttggctacc aattcatagg aggattcaag atgtctcata ctcagcttat caaagatacc
      181 ctaaatattc tagacctaaa tatctatttc gaggaaaatt gtttaactaa agaaaaatac
      241 aaagggcaaa tttgcttgat ttacaaagga actcttctgt acaagccaga agagtgttcc
      301 cattgtcttt gtgtcgtccc ctctaggatc attcgttggg ggacgacaac cgttcggttg
      361 ttactgaatg atgtgtctga gtatcgcact tatctggagc tgaagaaaca acgatttaaa
      421 tgtaagtctt gccaaagaac attcgtagcg gatacctctg ttgctgaaaa acactgtttc
      481 attagtcaaa aagttcgttg gtctgtcatt gctcgcttaa aagagaatac ttccatgacg
      541 gaaatcgctc gtcagaaaaa tatttccact tcttcggtct atcgtgtcat gaagcgattc
      601 taccgcccat tgaatccttt caaacagact ttgccaaagg tcttatgctt tgatgaattc
      661 aaatcggttc gaagtgttgc cagtgcaatg agttttatca tgatggacgg acaaagccat
      721 gcattattgg atattgtcga aaatcgacgc ttgccttatt tagaacgcta tttttctcgg
      781 ttttcattag cgactcgtga agccgttcaa ttgattgtta ttgatatgta tgcgccttat
      841 gtttcgttgg tgaaaaagct tttcccgaat gctcagttga tcatcgatcg tttccacatc
      901 gtccaacata ttggcagaac tttcttgaat catcgagtga aagaaaccac ggtacgactt
      961 aaaggaaatt cccactctca acgagggtta gggaaaaaat tgaagcgcta ttggaaactc
     1021 ctacaaaaag aagaagcaaa gttaaactat gaaaaacggg tttggcgtgc cagttttaaa
     1081 gattatttga ctgaaagtga gattgccgat cgactgttag ctgcttgtcc caacttacgc
     1141 caaggctatc agttatacca agacattctt tatgcggtaa agaaaagaga tcagtcacta
     1201 tttgaggatt gtttaactag agacattagc ggtctgccag aaacctatac aaccacttta
     1261 cgaacgttta aaaagtttct gccacagatc caaaatgcgc ttcactacag ctattcaaac
     1321 ggaccattgg aatgcttaaa caaccacatc aaagtattaa aacgcaatgc gtacggcttt
     1381 cgcaactttt acaatttcaa actacgaatt atgattcgtc atggcaaaac atttttaacc
     1441 aaataaacag aacagaaaga ggttttcttc tttctgttct gtcc
//
LOCUS       DACDEG010000210         1439 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.218, whole genome shotgun sequence.
ACCESSION   DACDEG010000210 DACDEG010000000
VERSION     DACDEG010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.218"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            276..1361
                     /gene="xerS"
                     /locus_tag="IV142_002823"
     CDS             276..1361
                     /gene="xerS"
                     /locus_tag="IV142_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP8486394.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT          564 a          240 c          206 g          429 t
ORIGIN      
        1 aaatcaaccg tagtctctct tatgtctaat tatatagaaa gattgttctt tctatataat
       61 ttagacaatt taaaaaatta agattgcctc cttctgtttc ctttcctatt catgtatacc
      121 atttatttta aattattaaa tctataccta tttatctgct tttttgaaat aaattaataa
      181 atttcagagt ataccaattg acaaaacact ctataattgg tatagtccaa ttatagagat
      241 actcctctcc cctacttatc gaaaggatga ataacatgaa aaatacagaa atatatcaaa
      301 aaatagatac aatcatactt catatgcccg attatataaa agattttgtt caagatcgtg
      361 aggataaaga tcaatcacct cgaactactt tagaatatct aaaaaattat aagctctttt
      421 atgaatggct tctgagtgag tctatcgttc ccgatacttc aatcacctcg ataagacagt
      481 taactgccta tgatctaaac ttgtacaaat ctcatttgaa acgtcgcaca aaggaaaatg
      541 taaaaaaaga gacaacaaaa actaagctca aagataataa taacttaggt ctatctactt
      601 ctactatcaa tagaaatatc acagcattaa aggttctatt taaatatctt tcgaaaagtt
      661 ccaataatcc tttaggaaaa ccatacttag aaaataatcc tatggatcaa gtagcaacta
      721 tcacagataa aactacgtta gctgcccgtg caaacgcaat cgagaaaaaa ttatttctag
      781 acgaagatac acaaaattat ctagattata ttgccaatga atataaaaaa actttgagca
      841 aacgagcttt gatctattat caacgagatg tagaaagaga cctggcgatc aatgcattga
      901 ttttaggatc tggattaaga ctctccgagg ttgtaaatat taatttagag gacctatctt
      961 tagaaaagaa taatgtggta gtaactagaa aaggaaataa gagagacgca gtaaatatag
     1021 cagcatttgc aatggaatat ttagctaatt atctagctat tcggaaagaa cgatacaaag
     1081 taacagaaaa cgaaaaagct ttatttctag caatttatca aggagaagcc aagcgtatat
     1141 ccggtattgc aatcgaacgg atggtagcaa aatattcaaa aggattcaga gtccaagtca
     1201 gtccacataa attacgtcat actttggcta ctagactcta tcaacagaca aattcattgg
     1261 tattaactgc ccaacaattg ggacatacaa gtacaaatac aacaacttta tatactcata
     1321 tagacaatgc agctacaatt gatgctttga acgctttgta aatgtagtaa aaaatagtat
     1381 aataatgaag cctgtctcta aaattagata aaagcccaac tttaaaggca agcttcatt
//
LOCUS       DACDEG010000211         1424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.219, whole genome shotgun sequence.
ACCESSION   DACDEG010000211 DACDEG010000000
VERSION     DACDEG010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.219"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(150..1337)
                     /locus_tag="IV142_002824"
     CDS             complement(150..1337)
                     /locus_tag="IV142_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP8486395.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DACDEG010000212         1412 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.220, whole genome shotgun sequence.
ACCESSION   DACDEG010000212 DACDEG010000000
VERSION     DACDEG010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.220"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..546
                     /locus_tag="IV142_002825"
     CDS             <1..546
                     /locus_tag="IV142_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP8486396.1"
                     /translation="ITLNDNGQSYADNFTIQGLLEYASRDSGYDGVGDLNRITGISRV
                     VDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSFGNLHYQYFHHIWLFIEVGILGVS
                     IFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVFFFVYNTTLRGETTGPLIYMILAM
                     PEVVKRKEHYYSEKNTKGEIL"
     gene            543..1262
                     /locus_tag="IV142_002826"
     CDS             543..1262
                     /locus_tag="IV142_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP8486397.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
BASE COUNT          489 a          158 c          283 g          482 t
ORIGIN      
        1 attacattga acgataatgg acaaagctat gctgataatt tcacaattca aggtctttta
       61 gaatatgcgt caagagattc tggatatgat ggtgttggtg atttaaatcg aataactggt
      121 atctctagag tagtagatga aatatttaat aataatttta gattagtcct aatgggagta
      181 ggcattggca atgcagagtt taccaatttt tttgtcagtg agttttataa atcgtttggt
      241 aatcttcatt atcagtattt tcatcatatt tggttattta tagaagttgg aatattagga
      301 gtttcgatat tcctattttt tataataaag acatttttca atgcaaaaag atcttttatg
      361 aaggataaag atgcaagtct tgttaaagtt attgcaatta tgtcggtatt cttttttgtg
      421 tataacacaa cacttagggg tgaaacaacg gggccactga tttatatgat tttagccatg
      481 cctgaagttg tgaaaaggaa agagcattat tatagtgaaa aaaatacaaa aggagagata
      541 ttatgattcc taagaagata cattactgtt ggttcggggg aaatccactt ccgaaaaaag
      601 atagaaagtg catagaaagc tggaaaaagt attgccctga ttatgagatt attgaatgga
      661 atgaatcaaa ttatgattta aataagaacc gttatatggc cgatgcatat aaagaacgta
      721 aatggggttt tgttccagat tatgcaagat ttgatattat ttataatcat ggtggatttt
      781 atttagatac agatgttgag ctaattaagt cgttagatat acttcgtgaa aatcatgcct
      841 atatgggatt tgaaggggaa atctgggtta atggcggtat tggttttggt gcagaagaag
      901 gacattctgt tattaaaggt ttaagggata tttacgattc attaagtttt tatcaaaatg
      961 atggtacatt gaacttgaaa ccgagtccgt attatattac agattatctt gaatctattg
     1021 gattggttcg cgataacagc gaacaaataa taaatggcct gcatatctat cctacggatt
     1081 attttgctgc caaggattat gatactggaa aaatttataa aactagtaat acaataagca
     1141 ttcatcaata taatgcatct tggaaaacac ctaaacaaaa atttgtttat agattaaaat
     1201 caacaattgg acaaaaaaaa tttaacaggt tacttgcact gaaaaaattt ttacacttat
     1261 aaaggagaaa agtactttga agaaaatttg tttcattagt ggtgtaattt ctcgttctgg
     1321 gggaactgaa agagtaggga gtattattgc caatagcttg tctgatttag gttatgatgt
     1381 cactatttta agcttttgga atagtggaga gc
//
LOCUS       DACDEG010000213         1406 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.221, whole genome shotgun sequence.
ACCESSION   DACDEG010000213 DACDEG010000000
VERSION     DACDEG010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.221"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            55..1008
                     /locus_tag="IV142_002827"
     CDS             55..1008
                     /locus_tag="IV142_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8486398.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            980..1255
                     /locus_tag="IV142_002828"
     CDS             980..1255
                     /locus_tag="IV142_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP8486399.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
BASE COUNT          508 a          206 c          254 g          438 t
ORIGIN      
        1 attaagtata tgaagtatac taaatatatt tagtatagga aattggaggg aactatggaa
       61 aacttacgga ctgcatttaa aacatcagat aaactaggaa aagttgtaat gcttacaggt
      121 agaaaaggcg gcattggtaa gactacagac aatgatttat tagcgattgt ttctagtcaa
      181 ctttttgaaa aagacgtgtt gctaattgat tatgaccaac aaagaaatac tacttccaac
      241 attggatcaa cgtatcaaat tactagcttt gatcgatcca tgtctgcagc aatcaaaaaa
      301 ggggactggg tttcaggtat tactcaagta agtccacatt tatatattat ggctggctct
      361 cctggatctg aagaacttaa tgaatacttg tccgaaaaat atcctgatcg tcgtaagaga
      421 tcattggcct tcattaaacc tttagaagaa ttgagaaaaa atttcgatta tatctttatc
      481 gactgcccac catcaacaga taatgtggtt cgtgcattct taactgctgc agattatatt
      541 attgcaatgc aagaattaaa acggtacgca atggaaggaa cagaagattt tattaataaa
      601 gttttagttc cgattgtcac gaattttgaa gagtctcatc ttcaaatcat tggaattttg
      661 cctgttcttt tctcagtgcg acgttcctct caacacgcaa actatcaaaa aacaattgat
      721 aagtatggtg aatcaaacat ttttaaaagc attgttaaag gctcggatcg gttagaaatg
      781 tatggtgaga atggtatcca attaaatgat tatgtagatc gaagatggtg ggctattttt
      841 gcagacatct ttacagaatt agaagaaaga attgaatact atgaacaaca tggtgatatt
      901 gaaggatttg attatacacc taaatatgct gactcaatgg ccaatcatat cctacctctt
      961 ggaaaggaga ttaaactaaa tggcattatt accaacgaac aaagataaga agaaaattgg
     1021 tgctgaagat atttcaatta aagatgatat taatgtcgag ttaggggaac gaaagtacac
     1081 tgcgaaagaa agaaaaccag ttcaagtaga tcctcccgta ctaaaaacca ttcgggatct
     1141 tagttatgca aaagatattc ctatgtatga aatagtcaaa cttgcaatgg acgcctatgt
     1201 tgatacttta aacgacgagg aacggattat atataataga cgatcaaaag attaaaatat
     1261 agtaagtata ctagatatag taaatatatt tagtatactt actatatttt tagctttatt
     1321 aatcctaatc cttcaaaata tgtgtattgt ttgggggtat acaacattca caaattttat
     1381 tgtttctagt ttttactata ctaaat
//
LOCUS       DACDEG010000214         1390 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.222, whole genome shotgun sequence.
ACCESSION   DACDEG010000214 DACDEG010000000
VERSION     DACDEG010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.222"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..204)
                     /locus_tag="IV142_002829"
     CDS             complement(<1..204)
                     /locus_tag="IV142_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486400.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESIS"
     gene            complement(221..>1390)
                     /locus_tag="IV142_002830"
     CDS             complement(221..>1390)
                     /locus_tag="IV142_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAP8486401.1"
                     /translation="HVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYDYKKLLTDNIKKT
                     KDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNLNVAMADRERLYN
                     SLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKTIEQIKIDNLLSQ
                     NYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVF
                     EKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIE
                     HLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKI
                     STAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT          493 a          230 c          158 g          509 t
ORIGIN      
        1 actgatagat tctctagctt cttcacataa actttttact ttattaatta ctactttgtc
       61 atattcattt aacacactat tattaccatt acttagacgc tcagattcta cgaaacaact
      121 caaatcagaa atttcccaaa aaaatttttt gttaaaatcc aatttcctca actcctccaa
      181 actacgtaaa tgatatagtc tcataataaa ataaaacctc tcagctaatg ttgttgaaat
      241 tgtccactaa acctaattga tattgcttag gagatacacc tgcgtacttt ttgaatactc
      301 tcgaaaagtg tgaagaatct gaaaatccta aacctaaaga aacttcactg atagtatggt
      361 ttgatttcaa cattatttta gccgtagaaa tttttcgctt agagatataa ttataaatcg
      421 taatatgcat ttcattctta aagctttctc ttaatttagt ttcacttaca gaaaaataat
      481 tagctatttc ctttacagta attcttttat agatatttaa gccgatgaat tgaacgacag
      541 aagaaattaa gggagaaaga tgctctatct tagtttttcc gatttcctta gtataataaa
      601 caatcccaga atccctcact tttaagacct caggtaacga tacagctaat tcgttttctt
      661 gtatcaaagc atctctactt tgatacgctt ctattatatc cattcctaat gttatagacg
      721 tttgtgctaa cttttcaaaa acaatgatag aataattttt ttcagaccga atcgtatctc
      781 cactaacact tggaattatt ccattactca gattaaaaac catgttttct aaatttcgaa
      841 tatctccatt tttaacatat tccagtattt tattttcata aagaaacaga tatatttctg
      901 gatcgtaatt ttgtgataac agattatcta ttttgatttg ctcgatagtt ttacttagat
      961 tttctgtata ttcaattaac ggttcatgga atagatcttc tatttttcca gtaaaaaaat
     1021 aatgaactaa aatcagaata tctcgaatgt ctcctaaaga atacaaagtc aaattttgca
     1081 atgaattata gagtcgttct ctatcagcca ttgcaacatt caaattgttc atttttagtt
     1141 taaaaaaaac tttatcaata ttatttgctc taaatggacc aaataagtaa tatctggttt
     1201 taaacatgta tagaagaaac atctcattaa aatcaccagt aaagaaatgg aaatctttag
     1261 tttttttaat attatcagtt agtaactttt tataatcata aaaaaaataa gtagttctat
     1321 ctgagcgata ctcttttata atactgaatt cggaattaaa aaccacgata ggtaacttta
     1381 aacaaacatg
//
LOCUS       DACDEG010000215         1373 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.223, whole genome shotgun sequence.
ACCESSION   DACDEG010000215 DACDEG010000000
VERSION     DACDEG010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.223"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            66..1025
                     /locus_tag="IV142_002831"
     CDS             66..1025
                     /locus_tag="IV142_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8486402.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            1158..1364
                     /locus_tag="IV142_002832"
     CDS             1158..1364
                     /locus_tag="IV142_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP8486403.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          481 a          218 c          272 g          402 t
ORIGIN      
        1 taaaaaagtc atttgtgcta cactcaaaat agttccgacc aaagaattat aaggagtgag
       61 tacaaatgac ctatacccat cttacaacgg atgaacttgt aataatagag tcttatttca
      121 aaatgaatca atctgttgct aaaactgccc attgcttgaa tcgttcaaga caaacgatcc
      181 ataaagtata tctgttcttc aagcaaggaa aatcagcctt agaatattat caacagtata
      241 agaaaaacaa atcaaactgt ggtagacgtc cgcttgtttt acccgaggaa caatcagaat
      301 atattcaaag aaaggttgtt caaggatgga cacccgatgt gattgttggt cgtgcagcgt
      361 ttcctattag ttgttctgct cgtaccattt atcgtatgtt caaaaagggg ctatttgatt
      421 cttctgactt accgatgaaa ggcaagcgta aaccgaatgg acatcaagaa agacgtggaa
      481 aacaaacttt ccgccgctct attcatgaac gtgaaaagga ttatagccaa ttctcaaatg
      541 agtttggtca ccttgaaggt gacactatcg taggtctgaa acataaaagt gctgtaatta
      601 ccttagttga acgattatca aaagttatca tcacattgaa accgtgtggt agacaagcga
      661 ttgatattga aaaaaaatta aatcaatggt ttgaatctgt accgaaaaac ctattcaaat
      721 ccatcacttt tgattgtgga aaggaatttt caaattggaa acagatcagt aatgtcaatg
      781 atattgccat ttatttcgct gatccaggaa cgccgtctca aagaggccta aacgagaatt
      841 ctaacggatt gttacgtaga gatggtttat tgaaatctat ggatttcaat tcagtagatg
      901 aattttttat tcaatctgtc gcatctaaac gaaataatat tcctagaaaa tcactggatt
      961 atcgaacacc tttggaagta tttttgagtt acgtaagtat tgatgatctg tctaacttaa
     1021 tttgacaatt aagaaagata aaattgaaca actggacggg caaattgatg ccttgctttc
     1081 agaaagagaa atattgatag aagaaaacaa gcgattgaaa aagaaagtag cccaaccaat
     1141 gaagggaggt ggatagtatg aacaatagaa cctttgatat tttgaaatgg atcgctatag
     1201 tagttatccc cgctttagcg acttttgtcg gtcttgtcgg aaaaggtgtt aactggcggt
     1261 acacagatat aacggtgatg attattacag gttttggagc atttctaggg agtattttag
     1321 gtgtatcaaa tcgaacgtat aagaaaattt catcagataa ataggaggat aga
//
LOCUS       DACDEG010000216         1355 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.224, whole genome shotgun sequence.
ACCESSION   DACDEG010000216 DACDEG010000000
VERSION     DACDEG010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.224"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1073
                     /locus_tag="IV142_002833"
     CDS             <1..1073
                     /locus_tag="IV142_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8486404.1"
                     /translation="LQAELTAFLDYEKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTI
                     PRDRNGEFSQQTLPAYKRTNDSLETTIIQLFQKGITMAEISKLIEKMYGHHYTPQTIS
                     NMSKLVAEDVLAFKERTLEANYSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEV
                     LGFTIAPTESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCV
                     HVSRNIAHKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLN
                     PALLTFYNFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYN
                     QKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          473 a          227 c          224 g          431 t
ORIGIN      
        1 ttctacaagc agagctaact gcttttttag attacgaaaa atatgatcga aatggattca
       61 attcaggaaa ttcccgtaac gggaattatt cacgtacatt taaaacagaa tatggcgagt
      121 tgaatttaac tattcctaga gatcggaatg gcgaattttc tcaacaaaca ctaccagcat
      181 ataagcgaac caatgattca ttagaaacga ccattatcca attatttcaa aaaggcatta
      241 ctatggctga aatctccaaa ttaattgaaa aaatgtatgg tcaccactat acgccacaaa
      301 caatctccaa catgagtaaa ttggtggctg aagatgtttt agcttttaaa gaaagaacgt
      361 tagaagccaa ttattccgtt atatttatgg atgcgacgca tatccctgtg aagcgacaga
      421 ctgtttcgaa ggaagcagtg tatattacga ttggtattcg tttggatgga accaaagaag
      481 ttcttgggtt tactattgcc ccaactgagt ctgcttatat ttggaaagaa gttcttcaag
      541 atcttagaaa acgtgggtta gaagaagttt tattagtagt gacagacgga ttaagctgta
      601 ttgaagaaag tatccatagt gtgtatccga atgcccaatt tcaacaatgt tgtgtgcatg
      661 tatctagaaa tatcgctcat aaagttcgtg ttcgaggtcg aaaagaaatt tgtgaggatt
      721 tcaaattggt ttaccaagcg aattcaaaag aagaggcatt ggatcacatc gactttatga
      781 ctaggaaatg gaaaaagcag tatccaagag tcgtcaattt actcttgaat cctgccctat
      841 taacctttta taatttccct cacgccatca gacgaacaat ttattcgacg aacctgattg
      901 aaggctttaa taagcagcta aaacgatata ctcgaagaaa agaacaattc cctaatgaag
      961 aatctctaga gagattcttc atttctcaat ttaatcaata taaccaaaaa tttttaggta
     1021 gaattcacaa gggatttaaa gaaattcagg atacattaga gtcgatgatt taactgtaat
     1081 taacagaatg gattttccat ttacatataa ttcttgacgc taccataatc taaataacca
     1141 tcatattcat ctactactta tataattgag ttttattaaa ctttttaagt ttctattcta
     1201 tcaaatattt aaacaatctc tgcatccgaa aagactacct cgtatagttt tactaacaat
     1261 atttttatta gcgtgttcaa atcctgattt cttgtatcaa ttattttctg ttcactttca
     1321 ataagaaaaa agtctttact tccatggata ataat
//
LOCUS       DACDEG010000217         1352 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.225, whole genome shotgun sequence.
ACCESSION   DACDEG010000217 DACDEG010000000
VERSION     DACDEG010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.225"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            6..980
                     /locus_tag="IV142_002834"
     CDS             6..980
                     /locus_tag="IV142_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP8486405.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          467 a          180 c          225 g          480 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtca ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta acttattgct tttgcaaaat ttatttacct gttttctgga tcaacgatag
     1141 tgtatattat gctatccttg tttggatttt aggacgttta aagaggcact tttttacata
     1201 acttaatcca tagacctttt aagcatgctg tgagataata ataaagaaga gtttaaagcg
     1261 caccccaaac cacttcccca taagtgtatt acgctttaaa ctcttttata tttgaattca
     1321 ctaagaagca taccatattt ttgaaaaaaa gt
//
LOCUS       DACDEG010000218         1352 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.226, whole genome shotgun sequence.
ACCESSION   DACDEG010000218 DACDEG010000000
VERSION     DACDEG010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.226"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            82..>1352
                     /locus_tag="IV142_002835"
     CDS             82..>1352
                     /locus_tag="IV142_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP8486406.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFL"
BASE COUNT          459 a          260 c          257 g          376 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg at
//
LOCUS       DACDEG010000219         1351 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.227, whole genome shotgun sequence.
ACCESSION   DACDEG010000219 DACDEG010000000
VERSION     DACDEG010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.227"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(191..1231)
                     /locus_tag="IV142_002836"
     CDS             complement(191..1231)
                     /locus_tag="IV142_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP8486407.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFIPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAETESGISIIFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNSE
                     SFPKTITKDWLE"
BASE COUNT          402 a          212 c          184 g          553 t
ORIGIN      
        1 aaattaatta ttaactatta acacttatga cgtaaaaagc tacgtttgtg atttttatca
       61 taatatttta actcataaat tctattaaaa tagctatttc tttgtaacat ttgtaatata
      121 attagaataa taaaaaattt ttaattttaa tgctactcca tgaagcaaca atcaatttgt
      181 cagtgaaatt tcactctaac cagtctttcg taatagtttt tgggaagctt tcagaattca
      241 aacgtttttc acttgatact ttagcagcta ttggtttttc gataaaccaa ttttttacat
      301 agacaaaaat caggttctgg atattttcaa ctttttcggt ttttaatttt tgataggctt
      361 ttaacagtgt cgtgtataat tcttgttctg cattgacata ataactatcg agttcttcaa
      421 aaattattga aataccactt tctgtttcgg cttttttctt tgcattatga atagtctgga
      481 cagttttttg agcttcactg taacttgaag aaaatgtttt tattaaattt aatactcgtt
      541 cagggataaa cgtagcaatg ctgtcgtttg tcataattgt ctcaaaattg tctaacaaca
      601 attgatcttg taggcggtct ttttcagtgt ctatattgtg tctattagtg tctaatatat
      661 tattattaag ttctggtatg attttcatac cctctgattt atcaacgttt tggcacggta
      721 tgattttcat accctcttga gccccaaggg attcagcgtc tgatttttcg tcgagggtgg
      781 gtatgatttt cataccctct gatttatcaa cgttttccct attctctcta tcaaattttt
      841 cgagtaagta gatatcattt tctgagactt ttaattctgc tagatatagt ctattaggat
      901 tatttttccc taactgtttg ttaaatccca tctgcttttg taataataaa ccaaaggatt
      961 ccaactcttt tttgattgct atcgcttttt gtttttggca gtttaatacg ctacatagtt
     1021 catttgtcgt ataagtaaaa taaacattac cttcttcatc gatttgccgt ttgctgattg
     1081 cgatctctaa tcttgctttt aaaagcatat aggcaatctt agttgaatct ttcatttttt
     1141 tgtattcgtc agaaacaata aagacttttg gaaattgata aaataattcg ttgtaaactc
     1201 tatccgcctt aaaaaaatta aaatcagtca ttgtagatcc tctactttca cgttgtttta
     1261 tttatgtaaa attacctact cttttcgaaa atagcaaaga taaataaaaa atgcaaacgc
     1321 attaaaactg ttttttaatt aaatactacc t
//
LOCUS       DACDEG010000220         1321 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.228, whole genome shotgun sequence.
ACCESSION   DACDEG010000220 DACDEG010000000
VERSION     DACDEG010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.228"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(540..905)
                     /locus_tag="IV142_002837"
     CDS             complement(540..905)
                     /locus_tag="IV142_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486408.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(981..1226)
                     /locus_tag="IV142_002838"
     CDS             complement(981..1226)
                     /locus_tag="IV142_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8486409.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
BASE COUNT          449 a          230 c          252 g          390 t
ORIGIN      
        1 aagaaagcgt tattatttta gtctattttc agggggggaa ccttatgata aaaaaactat
       61 ttttactatc aatattaacg ctagcaatgg caggttgttc tgactctggc ttagcaactg
      121 aactgatcac aaaatcaact gaagccacga cagaggagtt aaaatgtgga aacgatcaca
      181 gtgactacag aggcttaaat cgacgatgca tgggaatatt aggcagaagc aattgtaagc
      241 atcgatgtag taatcgaaga aggtgtagaa gaagcaacag aaaaatcaaa agaaagatca
      301 aaagaagcaa tctgtgctgt gcatcgtata cagacgtcga agatgcggta cttttaaacc
      361 acgtcaggag atgttcattt atgtctaggg aagttggcga tgaaccaaaa ctagagtacg
      421 cttttagttt tgcacgtgag gatgaagata accctaaaac ttacgacatt ttagcttgat
      481 ctaagatggt taaagctttt taatgaagta agaggagata gaaggctttt ctagttatat
      541 taggtaagat aaattccttt ttgctccaaa tttgctaaac attctgctag ataaacttca
      601 tcgtgttccg gataaatcgg cacgttgcgc tctacagtag gtaaatcaaa aaatgcttcg
      661 catgatttgc cacgatacat aggatttttc cactctggtg ctgggctgta accacgtttt
      721 tccatttcat ccatgacaag ctgatgatat tggaataatc tataaggaga atagtgaaaa
      781 acataatcca cagtagcatg tttctttccc cagccatttc ctcttaatgc acagcattcc
      841 cgatgttgtc ctaaaagctg ttgacgagga agctttgaaa tcaatttttc atgccataat
      901 cgcatagaaa tgtctccttt atatatagat tgttaacctt agacgttaaa tgaaaaaacc
      961 agcaccaagg ctggaaaaaa tcacttcagt ttgcttgctg tttctgagcg accaattagg
     1021 taatcaacgg aaatatcgta aaagtcagcg atatagatta attcaatgat gcttggaatt
     1081 tcttctcctt cttcgtactt acgatatttg aataaaggaa tacttaacgc gctagaaaca
     1141 gctacttgag ataaagatcg gccagttctt aagaaattca gacgacaacg taaagtatcc
     1201 agtaaaatag cttcttcgat actcatttaa tcacctttca ttatagtaga atagttataa
     1261 tgactataga aagctacaca tgcaatcaat agtactcaat gttttttatt taaacgtatc
     1321 a
//
LOCUS       DACDEG010000221         1307 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.229, whole genome shotgun sequence.
ACCESSION   DACDEG010000221 DACDEG010000000
VERSION     DACDEG010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.229"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            223..1011
                     /locus_tag="IV142_002839"
     CDS             223..1011
                     /locus_tag="IV142_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8486410.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTTAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            998..>1307
                     /locus_tag="IV142_002840"
     CDS             998..>1307
                     /locus_tag="IV142_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486411.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNYKPKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     K"
BASE COUNT          526 a          188 c          226 g          367 t
ORIGIN      
        1 tcgcaaaaca tatttcttat gattaataaa aaattttaaa gtaataaaaa gtgattattt
       61 tactttcgaa taacatatga ataatcttta tatccgaacc gatatatatc tttaaaacaa
      121 aataatcaca caatattaca atataatgca tatacgatat tgcaatatca caatattatg
      181 atataattat attataatat aagatgaatg ggggatattt taatggcaag aatattgtca
      241 gtagccaatc aaaaaggtgg agtttctaaa actacgacta gcgttaattt agcagcatgt
      301 ttgaaatttg tttacggtaa aaatgtactt ttagtggaca ttgatcctca gggaaatgct
      361 acagataatg taggtttcga tatagatgga acaaaccaac ctacaattta tgaagtatta
      421 aaaggggagg ctgacattac agatgctatt ctggactata aaggaattga tgttttgcca
      481 gccgacatag ccctttcttc tgcagagagg gaatttactc aagtaggaag tgaacacagg
      541 cttaaaagag tacttcaacc aatcaaggac aactatgatt atattataat tgattgtcca
      601 ccaagtttag gaatgcttac agtcaacgca tttactgttt ctgatgaaat tatcattcca
      661 gttgaagctg cgtatttctc attaaaagga ctaataaagt taagtgaaac tattgaaacg
      721 gtaaaagaat atacgaacga caagcttgaa gtacgaggag tcctatttac taagtataat
      781 aagcgttata acatttctaa agaaatgacg actgcagcaa ccgaaatttc tagagtcatt
      841 ggtgcaaaaa ttttcaaaac ttatattcgt agaacaataa ccgttgacga agcgcaagct
      901 gctggatcag atctaatcaa ttttaataaa ccctcaacag ctgaagatga ctataaagca
      961 tttacagaag aatatttaaa agaagttggt gattttgatg gcagaagata atcgcagtca
     1021 agctgcagta agtagactac taggaaataa aaataaaaag aactacaaac caaaagaagt
     1081 agaagaaatc gaattaacca agaaaaatat aagtaaagtc ataaaagatc aaggaagtag
     1141 tgtaagaaca gcagatagca ttcgtataaa tccaattcta atgtcagcac tcaaatattg
     1201 gacgactatt gctgaatcag acaaaagtaa accagacgtt atagaagatg cgctattaaa
     1261 atccatacct gaagaatact tgattgaagg atataaacta gcaaaga
//
LOCUS       DACDEG010000222         1260 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.230, whole genome shotgun sequence.
ACCESSION   DACDEG010000222 DACDEG010000000
VERSION     DACDEG010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.230"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            108..500
                     /locus_tag="IV142_002841"
     CDS             108..500
                     /locus_tag="IV142_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8486412.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            501..1208
                     /gene="aac(6')-Ie"
                     /locus_tag="IV142_002842"
                     /pseudo
     CDS             501..1208
                     /gene="aac(6')-Ie"
                     /locus_tag="IV142_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
BASE COUNT          513 a          100 c          225 g          422 t
ORIGIN      
        1 catgatgaat ggatttattc ttcaagaaaa tacatcaatt ttgataagta gaaatggtaa
       61 aaacattgta tagcatttta cacaggagtc tggacttgac tgagtttatg gaagaagttt
      121 taattgatga taatatggtt tttgatattg ataatttaaa aggatttctt aatgatacca
      181 gttcatttgg gtttatagct aaagaaaata ataaaattat aggatttgca tattgctata
      241 cacttttaag acctgatgga aaaacaatgt tttatttaca ctcaatagga atgttaccta
      301 actatcaaga caaaggttat ggttcaaaat tattatcttt tattaaggaa tattctaaag
      361 agattggttg ttctgaaatg tttttaataa ctgataaagg taatcctaga gcttgccatg
      421 tatatgaaaa attaggtggt aaaaatgatt ataaagatga aatagtatat gtatatgatt
      481 atgaaaaagg tgataaataa atgaatatag ttgaaaatga aatatgtata agaactttaa
      541 tagatgatga ttttcctttg atgttaaaat ggttaactga tgaaagagta ttagaatttt
      601 atggtggtag agataaaaaa tatacattag aatcattaaa aaaacattat acagagcctt
      661 gggaagatga agtttttaga gtaattattg aatataacaa tgttcctatt ggatatggac
      721 aaatatataa aatgtatgat gagttatata ctgattatca ttatccaaaa actgatgaga
      781 tagtctatgg tatggatcaa tttataggag agccaaatta ttggagtaaa ggaattggta
      841 caagatatat taaattgatt tttgaatttt tgaaaaaaga aagaaatgct aatgcagtta
      901 ttttagaccc tcataaaaat aatccaagag caataagggc ataccaaaaa tctggtttta
      961 gaattattga agatttgcca gaacatgaat tacacgaggg caaaaaagaa gattgttatt
     1021 taatggaata tagatatgat gataatgcca caaatgttaa ggcaatgaaa tatttaattg
     1081 agcattactt tgataatttc aaagtagata gtattgaaat aatcggtagt ggttatgata
     1141 gtgtggcata tttagttaat aatgaataca tttttaaaac aaaatttagt actaataaga
     1201 aaaaaggttg agagtgtaaa atattttgtg taaatgaaaa aatccataca aaaaaggaag
//
LOCUS       DACDEG010000223         1200 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.231, whole genome shotgun sequence.
ACCESSION   DACDEG010000223 DACDEG010000000
VERSION     DACDEG010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.231"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            200..>1200
                     /locus_tag="IV142_002843"
     CDS             200..>1200
                     /locus_tag="IV142_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP8486413.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDSTVIAKIANTAKPRKINDST
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMQEDSINTFVPENVLNLIKTFSSSYSEAQQTVQTIHNAKKKAEELEQTIVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETF"
BASE COUNT          492 a          174 c          197 g          337 t
ORIGIN      
        1 agcttaaacc ctgaaataga gcggtttgct ttaatttatt ttttaaggta aactaaaaaa
       61 agataaaaaa agactactgc tttaacaaag tttggcgact tatcagcagt agaaaaatat
      121 atagtggata gatatatttg tttaattcat tgtaacatag ttttgaacaa aacgctatcc
      181 tacagaaaga ggattggcta tgaatgattt taattattac aaatcaaaag aaatttatcg
      241 tgaaaaatat tatcaaatgc ctaaggtatt ttttactaac gaaaaataca tggatctttc
      301 aaatgatgca aaaattgctt atatgctact taaagatcga tttgattatt cagtcaaaaa
      361 taactgggta gattctgatg ataatatatt cttcatattc acagttgaag aattaatgaa
      421 attgttacaa tgccgcgaag gtaaagtatc taagatcaaa aaagaattag aaaaagcagg
      481 tcttctgaaa cagaagagag gaagagtaaa taaacgtgat ggtaagattg aatctatgcc
      541 taatttattg tacttaggca agccagaagt tacgaatcaa gacgtcttta aaatcatgga
      601 agaggaagac aatactgatt ccaccgtaat tgcgaaaatc gcaaatacgg caaaaccgcg
      661 aaaaatcaat gattctaccg taattgcgaa aatcgcaaat acggcaaaat ccagtcatat
      721 caaggactca accgtaattg cgaaaatcgc agacaatcta ttctattcta attctataga
      781 cactaataga cacataatag acactgaaac tgaccagtta caaaatcaag ttttgctaga
      841 taacttcgtc gacatcatgc aagaggacag tataaataca tttgtaccag aaaatgtatt
      901 aaacttgata aaaacatttt cttcaagtta cagtgaagct caacaaaccg tccaaactat
      961 tcataatgca aagaaaaaag ctgaagaatt agagcaaact attgtcgttt atgaagaact
     1021 ggaatcttat ggaattgatg ctgacaaagg attatatatg actttactga aagcttatca
     1081 aaaacaaaaa actgaaaaag taaacaattt gcagaatcta attttcgttt atgtaaaaaa
     1141 ttggtttgtc gaaaaagcta ttgcggcaaa gcttgcaaac gaacaaagag aaacatttaa
//
LOCUS       DACDEG010000224         1199 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.232, whole genome shotgun sequence.
ACCESSION   DACDEG010000224 DACDEG010000000
VERSION     DACDEG010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.232"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            69..941
                     /locus_tag="IV142_002844"
     CDS             69..941
                     /locus_tag="IV142_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8486414.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          351 a          223 c          283 g          342 t
ORIGIN      
        1 aataatttaa atatgatata tagtcattta tttagtgatt tcattctaaa ataaagggag
       61 aaatgtctat gctatacggt gcaattgaag caggcggcac aaaatttgtg tgtgccattg
      121 gcgatgaaga aatgacgatc aaggaaagag tgagctttcc gacaacgaca cctgaggaaa
      181 caatgccttt agtcattgac ttcttcaaac aataccaagc agatttagca gggattggga
      241 ttggctcatt tgggcctatc gatatccatc gcgattccgc cacttatggg tacattacct
      301 cgaccccgaa attggcatgg caaaattttg atttcatcgg taccatgaaa aaagaatttc
      361 cgatcccgat ttcttggaca acagatgtca atgcggctgc ctatggtgag tatgtcttcg
      421 gcagtggcaa aggattgagc agtgtcgttt actatacgat cggtacgggt gttggcggcg
      481 gtgccttaca agaagggcgc tttatcgaag ggtttagtca tccagaaatg gggcatatgc
      541 tcgtcgtccc tcatccaaaa gatgactttg ctggcagctg tccatttcat ggcaactgtt
      601 tagaagggat ggccgctgga cccgccatcg aaaaacgctt agggaaaaaa ggacaagagg
      661 ttgcagaaga tgatccttat tgggaaatcg aagcctccta tattgctcaa tgtgcctaca
      721 atacaacctt gatgttttct ccagatgtga tcatttttgg cggtggtgtg atgaaacaac
      781 gccatatgct gcaaaacgtc cacgacgcat ttgcaaaatt ggtcaatggg tatgtcgaaa
      841 cacctgagtt aagcaaatat atcgtgaccc cagcattaga agacaacgct ggaacgctcg
      901 gttgtttggc attggctcga gaagcggtct tacattcata aacgaaaaaa ggctcttcga
      961 catttagtgt tggtagagaa agatacaact gaaaatttag tcataaccag ttaggcagag
     1021 tgcttaactg gtttttgttt tacttggaag atcaagcctt gaatgagata aattcttgct
     1081 cgaaagttat ggaaatttcg atagccgtag gccacttgta ttgtgcaata gttttttaca
     1141 aaaaaaaggg ggtgcggata tttttttgga cctttttaac aaatatatct ttgcttctt
//
LOCUS       DACDEG010000225         1186 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.233, whole genome shotgun sequence.
ACCESSION   DACDEG010000225 DACDEG010000000
VERSION     DACDEG010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.233"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            4..963
                     /locus_tag="IV142_002845"
     CDS             4..963
                     /locus_tag="IV142_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8486415.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIEN
                     ANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRSDVNQAYNTSGNVGYHVSF
                     NMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          397 a          224 c          239 g          326 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttatcaa gtcggtcaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc caacagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac gttggacact ccttataacc aaccgggaat caaatcgcat
      421 ttatgggtaa cacaaaacat ctggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcatat gatttagctc atggatttac cgatgaaaat
      541 ccaacaactt ctgaaaacaa gcctgtcatt gatccaacac gagctggtgc agctaatcct
      601 acactgacag atggaacgaa ttacgcccac attgatcagt ttggagaaat cgaaaatgca
      661 aatttgcatg tagctggatg gcacattgct aactataaat acgagtatat cttcattatg
      721 gattacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagatcagat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc cataatagtc acactccaag
     1081 ctatgcgata acaggtttgt tgccacacat tctactggtt gattgtttat ggctttatgt
     1141 ggcaacaacc agtaccctta gctcagttgg tcagagcaga cggctc
//
LOCUS       DACDEG010000226         1181 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.234, whole genome shotgun sequence.
ACCESSION   DACDEG010000226 DACDEG010000000
VERSION     DACDEG010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.234"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..154
                     /locus_tag="IV142_002846"
     CDS             <1..154
                     /locus_tag="IV142_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8486416.1"
                     /translation="NKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKV
                     LLGIQA"
     gene            206..352
                     /locus_tag="IV142_002847"
                     /pseudo
     CDS             206..352
                     /locus_tag="IV142_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
     gene            429..758
                     /locus_tag="IV142_002848"
     CDS             429..758
                     /locus_tag="IV142_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8486417.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            748..1035
                     /locus_tag="IV142_002849"
     CDS             748..1035
                     /locus_tag="IV142_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8486418.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
BASE COUNT          432 a          158 c          224 g          367 t
ORIGIN      
        1 caataaattc tatcgaagtt tacgcactgc ctctaccacg attaaaggca tggaagccat
       61 tcgaggatta tataagaaaa cccgaaaaga aggcactctc ttcgggtttt cggtctgtac
      121 tgaaatcaag gtattattgg gaatccaagc ttaaatcata gataccgtaa gggattttat
      181 tctttattta aaactttgca acagaaccca atcatttgag aagttaaaac ctacacatga
      241 aggtaggtat tttagaagat tgaatagaca acaccgggtt gtttataagg tagatgaaga
      301 tgaacacgtg gttgaaattt attcagcaag gacgcattac gagtcacagt aattacttgt
      361 aaatacatta tctcgtggta taatgtattt actaagtaat tacatactgt tagaaatgag
      421 ggatttaaat gattacaaaa acaagaaaac aaggcaattc aatcatgttg acagtaccta
      481 aagattttaa tgttcctaat ggagtcgaag ttgaagcaaa gttggtagag aatggtattc
      541 tttatgaatt tgttgaacca caaaaagaat tttatgattt tagcgaagat attttgtctg
      601 atataattgc agaaggctac gataaagatg agattcttgt agaatttaag aatagaaaaa
      661 acaaaatgca ttcttccttt agagatattg ctgaagatac acttactaat tctaaagtaa
      721 tgactaaaga ggagttggca acagaaattg gcttatgaaa tcgttccttc aaaacatgtc
      781 attaagtatt taaaaaagct aaaagagaag ccattgaaag aaaaattttt gaatttaatt
      841 tatgatgaaa ttgcgattga tccatataag ggtgaacaaa aaactgggga tttatcaggt
      901 atttggtcta gtgggttcaa atatgcgggc acaacatata gagtagctta cgagattata
      961 gaaaacaagg ttattccagt attgttatgt ggaacgcatg aaaactttta tgaacagttg
     1021 aaaaaattaa tctagtgatt tgttgtcgtt cttaagaagg acggcttctt ttttatcgaa
     1081 aatctgattc gtctacatga ctaaagtcgc gagtattctc tgcttctcga taaattaaat
     1141 aacgtcatta ctttgaggaa agctataaat caatatattt c
//
LOCUS       DACDEG010000227         1146 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.235, whole genome shotgun sequence.
ACCESSION   DACDEG010000227 DACDEG010000000
VERSION     DACDEG010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.235"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            2..262
                     /locus_tag="IV142_002850"
     CDS             2..262
                     /locus_tag="IV142_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486419.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(624..863)
                     /locus_tag="IV142_002851"
     CDS             complement(624..863)
                     /locus_tag="IV142_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486420.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          401 a          164 c          212 g          369 t
ORIGIN      
        1 tatgaataac gaaactatca ttttaggaaa aaaatatatt tgccaaccta ttggcttgaa
       61 acaaaaagtg gttggagaag taatcagtaa attagaaaat tgtgttgtga tctgcgtaga
      121 cgaatatcat gacgtcgatc gcaatgaaat attagaaaaa tgcggaaaag tagtggcaaa
      181 atatactcac gtttatgagt tggaaacaac agaatatcta tataactata gcaacaaata
      241 tgaacatact ttagtttcat aaagatttaa ataaagtgcc ttcaaaagcg agaagaccaa
      301 gcttttgaag gcactttatt tagttggcag tgattgttca tgatttttga tttattcaaa
      361 aggttcgcct agagaatctg tctctgtaac gaggcctggt ggagaaactc gataaaaacc
      421 tatactgcca gaactgtcct attttcggat agacaaacta gtagttttaa aaagatcatc
      481 cgatccaatc atttcaaaaa atgcaaaact gatatcagga tcatttagct caggcttgtt
      541 atcgtaaagc atttgtaaag gttgcattta gttaggagta aacgcgaaac tatcatcatt
      601 atttgctgaa gaagatgaag aagctaattc agagtttgtt tgaaataaag gagacgtgct
      661 gacagtttct ttaccctgtt ttttgcagag gtagaaactt ttatatcgga agtttctacc
      721 ggagaatttt tctcatttgt agaaattgtt gaatcgatac tattcgaaat ttgcgtatct
      781 gaaggtatgt tctttgatgc agaagtccca caagcagtaa gtaacaataa atttgataaa
      841 ctgataaaca gaattctttt cattatgtac acactcctta tagtatcgtt tagcatacac
      901 ctatgctcag tattaggcaa aatcaatgca aaactatcaa gtcttttata gtatctatta
      961 gtcttagtaa tacatatatg gagagggtga tatcccagtt caaaccagat acattggtta
     1021 ccatctgtat taacgattat gcctctaaac gcttttttta tgaaaaaatt tataagtgaa
     1081 aacgtaggta tgatattatc tagaggtaat ttttttgggt atttaaatag ttgctaggag
     1141 gatagc
//
LOCUS       DACDEG010000228         1121 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.237, whole genome shotgun sequence.
ACCESSION   DACDEG010000228 DACDEG010000000
VERSION     DACDEG010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.237"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..83
                     /locus_tag="IV142_002852"
     CDS             <1..83
                     /locus_tag="IV142_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP8486421.1"
                     /translation="WLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            88..1035
                     /locus_tag="IV142_002853"
     CDS             88..1035
                     /locus_tag="IV142_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP8486422.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          358 a          170 c          239 g          354 t
ORIGIN      
        1 aatggttaga atttttgttt cctaaatttt tagaagattc ttcggagctt caagatatct
       61 ataattattt atatcggagg taaaaggatg agctgttatc ttatcaccgg tggggctggc
      121 ttcattggtt ctaccttagc caatcattta ggagaagaac atgtggtaat cgtcgtcgat
      181 gacttatcaa tgggaaaagt agaaaatctt gatatgacga aaaatatcac ttttattgag
      241 ggagatgtcg ctgagaaatc cttgatggaa gagatcatga aagctaataa atttgattat
      301 atctttcatt tagcagcagt tgctagtgtg gcagattcag ttgcccgacc agtagaaaca
      361 catcgtgtga attttgaaag tgtattaatg attttagaat tgatccgcaa gtatcaacct
      421 gatttaaaac ggatcgtgtt tagttcttca gcggctgttt atggggatga accgacttta
      481 ccaaaaaaag aagagtccgt gattcgccca ttgacgcctt atgcggttga taaattcgct
      541 gcagaacaat atgtgctaga ttattgtcat ttatatgatg tacccggtag cgcagttcgt
      601 tttttcaatg tctatggtcc aaatcaaaat ccgaattctc catacagtgg tgtgatttct
      661 attctcgtag atcgctataa gaaacaaatc gtaggagaag caacttcatt tacattattt
      721 ggtgatggaa gccagtctag agactttgtt tatatagatg atgtaatcca agcattactt
      781 ttagttgcga atgaggaagc tgcactgggg aaacaattca atgttggaac gggtaaagca
      841 accacacttt tggaattgat tcatgcaatc aatgaaattc tcggtgtaga attgacactt
      901 gaatacaaag aagagcgttc gggtgatatc catgactctc tagcagatat tactaagatc
      961 cagtcaattg gctatcaacc gaaatatggt attttaagtg gaatgactaa ttatttgaaa
     1021 actgaaatca attaaaacat actaagataa gattagaggg taaattcagg ctctttgtca
     1081 ataaggactg atgagttttg taaaatcaaa tctgggatag a
//
LOCUS       DACDEG010000229         1120 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.238, whole genome shotgun sequence.
ACCESSION   DACDEG010000229 DACDEG010000000
VERSION     DACDEG010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.238"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            346..981
                     /locus_tag="IV142_002854"
                     /pseudo
     CDS             346..981
                     /locus_tag="IV142_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(996..>1120)
                     /locus_tag="IV142_002855"
     CDS             complement(996..>1120)
                     /locus_tag="IV142_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8486423.1"
                     /translation="NQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
BASE COUNT          376 a          199 c          205 g          340 t
ORIGIN      
        1 tagttatata tttaaatttt taccagaaag aaatgaacac cttcaattat ctaaatctcg
       61 aaaatggtta gatgtaatca tcaggaaaaa caatcttaaa cgaattacta ttcatggtct
      121 tagacacaca catgctagct tgcttttagc aagctagtgc aaatattaaa gatgtgcaag
      181 agcgtttagg ccactcttct attcaaatca ttatggatct atatattcat attacagaca
      241 aaagaaaaga agaaaccgta gcgcaattcg caaaatatag gctctataat ttatgggact
      301 gatgaatcaa aattgattct tcagtcccat tgttatttta ggtatatgat tcgtacattg
      361 aaagagttga aaaactatct tccgaagccg tcaaaaagga ccctgccaaa agtattgatg
      421 gtggatgaat ttcgttcgca cgcttcaatt gtggataaaa tgagctttat ttgtgcggat
      481 ggcgagactg gaaaactcat tgatattttg cctacacgaa aattgtctcg attaacacac
      541 tattttagtc gctgtgcaaa tcctgaagcg gtggaatatt tagtgacaga tatgaacgcc
      601 gcctacttcc aactgacaaa acgtgtctta ccaaatgcga aagtcgtcat tgattgtttt
      661 catatcatca aacatttgaa tcaggcgttc aatgattttc gtgtacaaga aatgaaaaga
      721 ttacaccagt tagggcaacg acgggaagcc gagaaagtga aaaagaactg gcgctttctt
      781 ctaaaaaacc gtcaaaaaat caatcactat gattacaaaa catggcgcag ttttcgtgca
      841 cctaaatacc cgctactcac tgaagcaatg atgattgatc gattgctggc attttcaacg
      901 cctttaaagg acgcctatac ttttttccat gagctgaccg aagcctttag agacaaggaa
      961 tcagacttat tttttacctt atattatttg tcaaattaag cgagacaaaa ctttccattt
     1021 acgtaactca aaaaaacttc taatggtgtt tggtaattta gtgattttct agggatatgg
     1081 tttcttttgg acgcaacgga tgagatgaat ccttgattga
//
LOCUS       DACDEG010000230         1108 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.239, whole genome shotgun sequence.
ACCESSION   DACDEG010000230 DACDEG010000000
VERSION     DACDEG010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.239"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..546
                     /locus_tag="IV142_002856"
     CDS             <1..546
                     /locus_tag="IV142_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8486424.1"
                     /translation="NVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRMSDI
                     IGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQGRH
                     ITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQAITK
                     RYIGITEDEIGASLRGFKLGG"
     gene            800..>1108
                     /locus_tag="IV142_002857"
     CDS             800..>1108
                     /locus_tag="IV142_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486425.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     L"
BASE COUNT          451 a          113 c          204 g          340 t
ORIGIN      
        1 aatgtacaac cgttaaggac aaatgaagag atacaagatt ttcagttttg gctaagaaga
       61 acaagtaatc cagaaagaga tagtttttta tttttgtttg gaatcaataa tgggttacgt
      121 atgtcagata ttataggatt aaaagttgga gacatacgag gtaaaagtaa gcctattatt
      181 gttgaacata aaacaggtaa gcgaaaaccg atttttatag ataatttgag agaagagatt
      241 ttattatata ctgaaggtaa agaagaaaat gattggttat ttccaagtcg gcaacaaggt
      301 cgacatataa caagggatcg tgtctatcag atatatgcag atatagcaga aaaattagga
      361 agagatgata taggaacaca tacattaaga aaaacttttg ggtatcacta ctacaagaaa
      421 actagagaca ttgctacctt aatgtttatt tttaatcata gctcgcaagc aattacaaaa
      481 aggtacattg gtataactga agatgaaata ggggcatcgt tacgtggttt taaattggga
      541 ggctaattga gatattagga tatatttttt gtgtataatg aaaacttaga aggtgagatt
      601 aatgaagata actgaatttt cggaagtatt taaaacgttt agtaacattt acaatagaat
      661 accagagata gaacaaaatg ttgtagttga taatgattat tatatagaga atgattttaa
      721 taaaccaaga aaatatgaca acctatacaa ttttatacac aatagaacaa caataaataa
      781 caacagtaac actcgagttt tgattaattc agcatatttt catattaaat atttaaagaa
      841 atatgagtat gaagaagttg tattagatta tgggctaaat agacagtcat ttttaattta
      901 tgcaataaat aacggaaatt tgccaagcaa taaaaagaac ataattattg aaacatatgc
      961 ctatctggag gggggatcta aaaaactaat taagtcatgt aatatagagt atcaattatc
     1021 agtaggagaa attaaatgtg ttttcgaaga agatttagaa cttgacttaa aaaaaatttt
     1081 tgccagcatt catgattata taggtttg
//
LOCUS       DACDEG010000231         1074 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.240, whole genome shotgun sequence.
ACCESSION   DACDEG010000231 DACDEG010000000
VERSION     DACDEG010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.240"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            854..>1074
                     /locus_tag="IV142_002858"
     CDS             854..>1074
                     /locus_tag="IV142_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP8486426.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLK"
BASE COUNT          373 a          147 c          185 g          369 t
ORIGIN      
        1 aaaattggtt agagtagttg ataaaaagca attactataa aaatgatcaa ttttaaatta
       61 gaactttttt atgattttaa ttttattaca aatattacaa ataaatagtt agtattccgg
      121 agtttttaga ttaaaagttt atgaaaacaa tcacaatcgt aggttttaaa ttcataatcg
      181 ttagatattt tagttatttt tcactcaaaa aaatagggaa ctagtgtttg catatgtgct
      241 tttttgagtt ataataattc atgaagagag gtaattcact tatttcccca agctgaaaag
      301 cctgatatta cttcttttca ggcagtgctg ctaatattca attacacttt tttgtatatt
      361 tttagttgtt ttaaatttaa ttgtctgcta tgcttgtgat gaattgattt tcgttctgca
      421 cttttggacg ataaatttgg acaattgaaa atacaaaaaa aagaaaggcc ctgtgagact
      481 cggaaggctg gtaaccttcg tcgacgtcca caggtggcat taagtctata ccccttaatg
      541 cgtttacctt tcagtgttac ggtattaccc gtaacggatt tagtataaca gatattcaaa
      601 agtgatacca ctttttttgg ctgtgttaag gttaaaaata cgtgatatta cgatgaatct
      661 atgcctaagc ataatgttat aaaaatgtcc gtggggctct ttgccgtatg acgaacgctg
      721 aaattagagg gaacgcatgt ttgaatgcgt tccctctttt ttgtttgtca tgaacgaaaa
      781 tcaattctct atttttgaaa aataacaaga aaaacttgtt aaaaatattt aaagtaagtg
      841 ggtggattat tttatgtcat atacaggaat tttgagtctc gaagatattt gtcactacgg
      901 taaacggtgt accgctacag aaaagatcac gaagaaacta tcaactggtc aaagtaaaac
      961 cgttgtgcag tgtaagaaat atataatcca gaaagacaaa gtctcagaag aaatgattta
     1021 ttacattggg aaacaaaaac aaataatttt aaaagatcct atccctttaa aaga
//
LOCUS       DACDEG010000232         1035 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.241, whole genome shotgun sequence.
ACCESSION   DACDEG010000232 DACDEG010000000
VERSION     DACDEG010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.241"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>1035)
                     /locus_tag="IV142_002859"
     rRNA            complement(<1..>1035)
                     /locus_tag="IV142_002859"
                     /product="16S ribosomal RNA"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DACDEG010000233         1023 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.242, whole genome shotgun sequence.
ACCESSION   DACDEG010000233 DACDEG010000000
VERSION     DACDEG010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.242"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            347..733
                     /locus_tag="IV142_002860"
     CDS             347..733
                     /locus_tag="IV142_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8486427.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            827..1018
                     /locus_tag="IV142_002861"
     CDS             827..1018
                     /locus_tag="IV142_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8486428.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT          388 a          150 c          160 g          325 t
ORIGIN      
        1 gttaattatg aagggggaag aaataaaaaa tacaatctat acacgaataa gaatattatc
       61 caaaaggagg ttttagaaaa gatttattat acgtaaaaag ctcaagacat tttcttagcc
      121 ataaggacat gtttgatatt taagaagata tatgaatttt cttttctaag tataaagata
      181 actatccatt atcaactaaa aaaacatttt tgaaaataaa catcagcagt cctatcaatg
      241 catgtgagct tatagaagtt attcgcagcg cagcataatt gcattagctt cttttcgtgt
      301 tttttgcctc ttttcccttt aaatcttaat ggaggaggtg tgcaatatgt cagataacga
      361 cgaacgcgta aatgcccagt tgattcgatt ctttgaaaag gtcattttga acaccgctaa
      421 tacttattat aaaaaacaag aagaaattca aaaacatgaa caacttgatc aactcccacc
      481 atatttgttt actaaagata ctataaaatt taaacaaccg attaatgttt taaatgtgac
      541 tctccttgtt gaagatataa aattagcaaa aatccttcct ttgttgaaag aacctgaaca
      601 aatttttctt atcgaaaaat ttatctttga taaaactgat aaagaaattg gaaaggttct
      661 ggggattaca agacagggtg cgacaaattt aaaacatcga ctttacaaaa aaattcaaaa
      721 aatgctggtt taatatctgc ataataacta attttgtatg cttctttgct cgttctcctc
      781 cttcccaaag aaatagatta taagtccatg ggaagaaggt agatttatga attctttaaa
      841 agaagatttc attttagcaa aggctggaaa tgaagaagct gttgaggcaa ttctcaaaag
      901 atttagctct ttgattcata agcaatcctg gagaactgga aaatatgatc aagattgtta
      961 tcaagaatgt atgcttgcaa tatatctagc tatctcgaaa ttcgaaatta aagaataact
     1021 caa
//
LOCUS       DACDEG010000234         1014 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.243, whole genome shotgun sequence.
ACCESSION   DACDEG010000234 DACDEG010000000
VERSION     DACDEG010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.243"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>1014
                     /locus_tag="IV142_002862"
     rRNA            <1..>1014
                     /locus_tag="IV142_002862"
                     /product="23S ribosomal RNA"
BASE COUNT          314 a          200 c          293 g          207 t
ORIGIN      
        1 ggagctgtac gtaagctatg atccagagat ttccgaatgg gggaacccaa catctttaat
       61 aggatgttac gattgtgtga atacatagca cattcgaggt agacgcagag aactgaaaca
      121 tctaagtacc tgcaggaaga gaaagaaaat tcgattccct gagtagcggc gagcgaaacg
      181 ggaaaagccc aaaccagcaa gcttgcttgt tggggttgta ggactccaat atggtagttc
      241 tttcagatag tcgaatgact tggaaaagtc agtcaaagag ggtaaaaacc ccgtagacga
      301 aatgtggaag acacctagga ggatcctgag tacggcggaa cacgagaaat tccgtcggaa
      361 tccgggagga ccatctccca aggctaaata ctccctagtg accgatagtg aaccagtacc
      421 gtgagggaaa ggtgaaaagc accccggaag gggagtgaaa tagaacctga aaccgtgtgc
      481 ctacaacaag tcaaagcccg ttaatgggtg atggcgtgcc ttttgtagaa tgaaccggcg
      541 agttacgatt gcatgcgagg ttaagttgaa gagacggagc cgcagcgaaa gcgagtctga
      601 atagggcgtt tgagtatgta gtcgtagacc cgaaaccatg tgatctaccc atgtccaggt
      661 tgaaggtgcg gtaaaacgca ctggaggacc gaacccacgt acgttgaaaa gtgcggggat
      721 gaggtgtggg tagcggagaa attccaaacg aacttggaga tagctggttc tctccgaaat
      781 agctttaggg ctagcctcgg aattgagaat gatggaggta gagcactgtt tggactaggg
      841 gcccatctcg ggttaccgaa ttcagataaa ctccgaatgc cattcattca tatccgggag
      901 tcagactgtg agtgataaga tccatagtcg aaagggaaac agcccagacc accagctaag
      961 gtcccaaaat atatgttaag tggaaaagga tgtggggttg cacagacaac tagg
//
LOCUS       DACDEG010000235          987 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.245, whole genome shotgun sequence.
ACCESSION   DACDEG010000235 DACDEG010000000
VERSION     DACDEG010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.245"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..144
                     /locus_tag="IV142_002863"
     CDS             <1..144
                     /locus_tag="IV142_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP8486429.1"
                     /translation="SAIIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRD
                     GER"
     gene            141..671
                     /locus_tag="IV142_002864"
     CDS             141..671
                     /locus_tag="IV142_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAP8486430.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
BASE COUNT          364 a          121 c          201 g          301 t
ORIGIN      
        1 tcagcaataa ttgaattttt acaacttttt ttgaggctag gaacgtttca aatttcggat
       61 tttgtatata atacgttggg cggtatattg ggtgtaatcc tttataaact aattttgatt
      121 acaaaaagag atggtgaaag atgagactcg gattgataat aaagaatata ttaaatttac
      181 ctaaaacgat atacttcaat tataaatttc ttcatatttc agatgcgtgg agattaccta
      241 ttttggtgca tcgtaaagta cggattacac gcaaatcgag agggaaaatt gagatagtat
      301 tacaatctgg aaaaatagtt tatttgggct ttccagtatt aaatactgcg ggaaatgaag
      361 aaacgattat aagtattgaa ggaatactaa gaatacaagg gccagcgttt atcgcacaag
      421 gggctcatat tgacataagg aaaaatgcag ttttagatat tgggtcaaag tttaattcga
      481 caggaaaaat gagtataatt tgctttaagg aaattcaatt caaagatggc tgtacagtat
      541 cgtgggatac attatttatg gataccgatt cacatacgat ttttgaattg aatactcata
      601 ggattttgaa taaaaataat aaaatagtta taggtaaaca ttgttggatt ggagcaaaaa
      661 gcgtgatata gaaaggtgtt catttagcag ataatattgt tgttgcttgt aattccacgg
      721 tagtaaaaag tcatgaaaat aataatgaga ttataggggg ggctccagtt cgaacgttga
      781 aaagaaatac aagttggtgt ttgaacgagc caaagtgaaa ccaaataatt ataaaactat
      841 ggaagtataa taaagttttt gagaaatttg aacgacaaga ccctaaaaaa atccatggac
      901 cattattatg gatggacttc ccaaaaggaa tagccacttt tgagcacgta aatcccctaa
      961 taagtggatc gacatcgctt gtgcagc
//
LOCUS       DACDEG010000236          979 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.246, whole genome shotgun sequence.
ACCESSION   DACDEG010000236 DACDEG010000000
VERSION     DACDEG010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.246"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(53..787)
                     /locus_tag="IV142_002865"
     CDS             complement(53..787)
                     /locus_tag="IV142_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP8486431.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
BASE COUNT          339 a          203 c          181 g          256 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcac
//
LOCUS       DACDEG010000237          973 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.247, whole genome shotgun sequence.
ACCESSION   DACDEG010000237 DACDEG010000000
VERSION     DACDEG010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.247"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            68..808
                     /locus_tag="IV142_002866"
     CDS             68..808
                     /locus_tag="IV142_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8486432.1"
                     /translation="MTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGL
                     TTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTP
                     GGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVET
                     QAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGE
                     TIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT          346 a          168 c          245 g          214 t
ORIGIN      
        1 ggggtatgaa ttagatgcga caccgatcga gtttgagatc gaaagaagtc agacagcggt
       61 ggtagaattg acgaaagcaa accgacttac accaggcggt gtcgtgttga caaagatcga
      121 tgaccaaagc ggggaagtcc tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
      181 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgattg acggcttagc
      241 accaggagtg tatcagctgg tagaaaccca agcgccaacg gggtatgaat tggatgcgac
      301 accaatcgag tttgagatcg aaagaagtca gacagcggtg gtagaattga cgaaagaaaa
      361 tcgacttaca ccaggcggtg tcgtgttgac aaagatcgat gaccaaagcg gagaagtcct
      421 gcaaggagct gtttttgagt tacaagatgc caatggaacc gttctgcagt caggtctaac
      481 cacaggagca gatggaaaac tggcgataga tggcttagca ccaggagcat atcagctggt
      541 agaaactcaa gcgccaacgg ggtatgaatt ggatgcgaca ccagtaactt tcaagatcga
      601 aaaagaacaa gaagtggcga tctttttaac aaaagagaat agaaaagttg ctgatagctc
      661 agatataaga ggaaacaatc caacaagcag tggaaataag catcttccaa aaactggaga
      721 aaccatcatt agccagttta tattgtcaat attgggtgtg ctacttgttt tcatcagtat
      781 aaaatttaga aagaaacgaa atatatgagc taaaataaaa aattgaataa gcatagtttt
      841 taaaatcaac tagtacccat caattgaatg gaaactaccg attttagaag gggtatacaa
      901 acaaatatta gtatcggagt agagaaggtt gaacccaacc agtaaataag taaagacaat
      961 tttgtgctgc gtt
//
LOCUS       DACDEG010000238          945 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.248, whole genome shotgun sequence.
ACCESSION   DACDEG010000238 DACDEG010000000
VERSION     DACDEG010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.248"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            70..840
                     /locus_tag="IV142_002867"
     CDS             70..840
                     /locus_tag="IV142_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8486433.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
BASE COUNT          363 a          152 c          166 g          264 t
ORIGIN      
        1 atgaatgttt aaatgaacaa ctagtcaaat aaatccatgg atcaagatgt ccctcaggag
       61 gcaaaagtca tgaaaaactc acttgtcaat attgaaaaac gtcaaaaaga aattttggaa
      121 ctgcttcagc agactgaaca actatcaact ttcgaattag ctgaatccct tgatgtatct
      181 attagtacga tacgaagaga tttaaattta ttagaagaaa aaaacgagat catacgcaat
      241 tacggttatt gttcttataa ttacaaaaac cagactgatt ttgatcaatc tggtcctgaa
      301 aggattaaac aagcgattgc cagagaagct agtaaataca ttaacgatta tgatacgctg
      361 tttatcaatt ctagctcgac tgcgttgaaa acactggatt ttcttcaatc taaccatttg
      421 acagtcatta caaacaacgt gaaaatcaat tactctccgc acaaagataa ttgtagttat
      481 attttaactg gtggagagat gcgctttcca aaagaagcac tagtgggcga catcgcatta
      541 aatacgattt cttcagtcaa tgcagacatt tgtatcattg gctgtagcgg agtcgatgta
      601 gtacatgggg tcacaacaaa gatattaaat gaatcaacga tcaacgaaat gatggtcaac
      661 aagacaatca aatgtaagat tttagtcgct gatcatcgaa aaatcggact gacatcaaag
      721 ttcaagattg ctgatatttc agtctttgat tatttgatca ctgataaatt ttgttctgaa
      781 aaagtattag aggagatcag aaatacgggg ataaaaacca ttcaagtaga ttcattttaa
      841 gaaccaggac gtaaatatgt aaataggatt atcaaaaaaa tgaataacaa atttatcgat
      901 aaatgaaaaa cacgctacgc tcaaattgat aaaggaatcc caaga
//
LOCUS       DACDEG010000239          926 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.249, whole genome shotgun sequence.
ACCESSION   DACDEG010000239 DACDEG010000000
VERSION     DACDEG010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.249"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..888
                     /locus_tag="IV142_002868"
     CDS             <1..888
                     /locus_tag="IV142_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP8486434.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          393 a          122 c          157 g          254 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt caacttttgg
      901 ggttcagttc atggaggggc aaggct
//
LOCUS       DACDEG010000240          920 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.250, whole genome shotgun sequence.
ACCESSION   DACDEG010000240 DACDEG010000000
VERSION     DACDEG010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.250"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..423
                     /locus_tag="IV142_002869"
     CDS             <1..423
                     /locus_tag="IV142_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP8486435.1"
                     /translation="MGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLA
                     TYILRYDDKIIEIHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESA
                     NDMYEKELKYFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            424..>920
                     /locus_tag="IV142_002870"
     CDS             424..>920
                     /locus_tag="IV142_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP8486436.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVN"
BASE COUNT          358 a           98 c          165 g          299 t
ORIGIN      
        1 atggggggag gagtagaatt agactgtata catgaattgg attatattat aaacctattt
       61 ggtttcccta aatcagtaaa aaaacagttt ggtaaatatt cgaatcttga aattaacagt
      121 aatgatttag caacatatat attgagatac gatgataaaa ttatagaaat tcatttagat
      181 tattttggaa gagttcctac aagaaaattt caattgttta ctttagatga atgtatcaat
      241 gttgacttat taaataataa aataagtttt gaagaaaaca ttgttaaaga aatgcctgaa
      301 tctgctaatg atatgtatga gaaggagttg aaatatttct ttgaaaacgt agtaacaggc
      361 aaagaaaact ggaatgattt atatcatgca aacgaagtat tgaaaatagc tgaggagaaa
      421 taaatggaaa ttttatttac tatttgtggt agagctgggt ctaaggggtt taaaaataaa
      481 aatttgaaat atttggcagg aaagccccta gtccatttcg ctttagcaat gatagatatc
      541 tatcaacaaa aacatcctga acataattgc acgacttgtt tgaatacgga cagtaatgag
      601 ttagtagaat tgacgactaa gttaaatccg tctgtgaaaa ttgtagaacg taaacctgaa
      661 ttagctacag atactgtagg gaaaattgaa gtgatacgtg attcattctt acaactagaa
      721 agagaagata atatatttga tttaattata gatttagatt tgacttcacc tttacgtgaa
      781 gtttctaatc ttgaagaatt aatagatgaa catctaaaaa atttagataa agacgtctac
      841 ttttcagttg tttctgcaag acgtaatccc tattttaata tggtaaaaag aattgaaaat
      901 gaagttacta tagttaataa
//
LOCUS       DACDEG010000241          908 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.251, whole genome shotgun sequence.
ACCESSION   DACDEG010000241 DACDEG010000000
VERSION     DACDEG010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.251"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(248..601)
                     /gene="tnpB"
                     /locus_tag="IV142_002871"
     CDS             complement(248..601)
                     /gene="tnpB"
                     /locus_tag="IV142_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP8486437.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(591..785)
                     /locus_tag="IV142_002872"
     CDS             complement(591..785)
                     /locus_tag="IV142_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486438.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          266 a          180 c          160 g          302 t
ORIGIN      
        1 cgtccaaata atttgttggt taaataattg accttttcat tcaatagttg gatttcagtt
       61 gtaagattct gaatggtttg ttgttgtgct tgattggttt tatgggcttc ttgaagcaac
      121 tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa ctgattttgt
      181 tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt tgtgttaacc
      241 ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga tcgatactta
      301 atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg tctttcgtcc
      361 gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa ccatcaccgt
      421 cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa acggcatctt
      481 cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca attccttttc
      541 gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa tcataaagca
      601 ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc gttatagatc
      661 aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc cgcgggttta
      721 cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc atttaattta
      781 tacatagaaa acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag
      841 taaggtactg ttctatgggg cgcttacttt ataaaggtcg tccaaaactc tatagtgcag
      901 atacaaaa
//
LOCUS       DACDEG010000242          889 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.252, whole genome shotgun sequence.
ACCESSION   DACDEG010000242 DACDEG010000000
VERSION     DACDEG010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.252"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..756)
                     /locus_tag="IV142_002873"
     CDS             complement(<1..756)
                     /locus_tag="IV142_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP8486439.1"
                     /translation="MMGNLVLSYNIYYFFITFLTMVVSYFSMKQIKNNRYISLLFSII
                     YTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQKWYWLSIGMTLVVYTH
                     LLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLSAGFLIPFIQQSRAQE
                     LKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLIGAFIFIKKLTADDLFIFF
                     LGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNG"
BASE COUNT          369 a          136 c          139 g          245 t
ORIGIN      
        1 accatttaac cgccagacaa attgtagact cttaactggt gtatggttaa aaagttgcca
       61 aggaaataaa ttggttgaac aaaaaagaac aaatacacca agaaagaaaa taaataaatc
      121 atcagcagtt aattttttta taaaaataaa agcaccaatc aatccgagaa ataagaataa
      181 tccaatagta tagttgttat agttattatt taaaatatgt gtcagcatat cactaggagc
      241 cataccactt aattcttttc ccagtgggac tttcaattct tgtgctctag attgttgaat
      301 aaaaggaatt aagaatcccg cacttaaaaa aaaggtgagg acggtagctt ttaaaagact
      361 taagagtcta gcgatttttt ggtcccaaaa ataaaaagaa agaaacaacg ttcctccaat
      421 aaatacagaa accatagcga cagaaagtaa atgagtatac actactagcg tcatgccaat
      481 agacaaccag taccattttt ggtaatctct aatataaatt tcgtaacacc ccattagtat
      541 caaaggcaaa aatgttaaag cgacagcttc tcccaatgaa gcacgatgga atatgtcaat
      601 ggcacgatag gcggaaaatg tataaataat cgaaaataac agcgaaatat aacgattgtt
      661 ttttatttgt ttcatagaaa aataagaaac gaccattgtt aaaaaagtga taaaaaaata
      721 gtagatatta taacttaaaa caagatttcc catcatttta taaaataaaa aagctggata
      781 aagagtaagc catggataaa aaatattcat cattgttcca tgatgatcga agttattgaa
      841 gtttactgga gaactccata cattatctag tcctatgatt cgagataaa
//
LOCUS       DACDEG010000243          875 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.253, whole genome shotgun sequence.
ACCESSION   DACDEG010000243 DACDEG010000000
VERSION     DACDEG010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.253"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            209..281
                     /locus_tag="IV142_002874"
     tRNA            209..281
                     /locus_tag="IV142_002874"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:242..244,aa:Lys,seq:ctt)
     gene            443..664
                     /locus_tag="IV142_002875"
     CDS             443..664
                     /locus_tag="IV142_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486440.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
BASE COUNT          302 a          144 c          127 g          302 t
ORIGIN      
        1 tattttctat tgatctatct ttcagcaaat tataagcttt attttttcca atatttaaca
       61 ttttagttaa atcagtaatt ttcaatatcg atggataact ttcaaatatt ttttcataat
      121 cttgaaaatc cataaaattc actctcaatt ctgataataa gtattgcact tttttatttt
      181 aggtgttaga ataacaaagt attaatttgg gctgttagct cagttggtag agcagatgac
      241 tcttaatcat cgggtcgcgg gttcgagccc ctcacagccc attgggtgcc aaacccttaa
      301 atacagtgta aacgttggtg cttagcattt agaaaacgta tatactgtat ttttttgttt
      361 tcaaaagaga ctgtagtacc cctttttttc actaaaagcc cactgtagtc attgatattt
      421 ttgataagaa aggaacataa ttatgcgtaa tgaaattgct aatataacaa taatccgaga
      481 aactttacaa aaccatactg caaatgagat ctctaaacat acaggattaa atttgagtac
      541 tataaaaaaa ctaaaaagtg gagaacgttc aatagaaaaa ctaaatttac atgatgctat
      601 ttgcctcact gaatttggtc ttaaaaataa tagaaaaaat gttgaaatca acatatggaa
      661 ataattctta atcagctgct tttgtcaaaa gcacatagga atttcacctc tctccaagtt
      721 tacggcgagc cttacggaag tatcattatc ccaacttcaa atcatcgctt tttattgact
      781 ataaaaatta ttttagaatg tttatcgctc cttctttaca gtaagaaatc atggcgcagc
      841 taaaatatgc tccttgaagt gttaacgtac tgata
//
LOCUS       DACDEG010000244          866 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.254, whole genome shotgun sequence.
ACCESSION   DACDEG010000244 DACDEG010000000
VERSION     DACDEG010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.254"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..294
                     /locus_tag="IV142_002876"
     CDS             <1..294
                     /locus_tag="IV142_002876"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAP8486441.1"
                     /translation="AAGIYEYEKVQIVDVNNGNRFETYTIAGERGTGMICLNGAAARC
                     VSTGDKIILMAYCELNENEVKDHRPKVVFVDDNNKVERVTSYEKHGRLSDIIL"
     gene            496..866
                     /locus_tag="IV142_002877"
                     /pseudo
     CDS             496..866
                     /locus_tag="IV142_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          347 a          119 c          167 g          233 t
ORIGIN      
        1 gcagcaggaa tatatgaata tgaaaaagta caaatagttg atgttaataa tggaaataga
       61 tttgaaactt atacaatagc tggagagaga ggaacaggga tgatttgctt gaatggagct
      121 gcagctagat gtgtatcaac tggagataaa ataattttaa tggcatattg tgaattaaat
      181 gaaaatgagg ttaaggatca tagacctaag gttgtttttg tcgatgataa taataaagtt
      241 gaaagagtaa catcatatga aaaacatgga aggttatcag atataattct ttaggttttg
      301 taatgttatt tatatatagc tagactatta ttgaaatatt tattgattac taattggata
      361 gatgtcaata aagtagacac aaaaagagaa gatacagact gtagacaaag tccattttat
      421 gtgaatcggc tacactagac tagacagaaa aaataaggtg tgtagaataa taaaaaacac
      481 actggaggat ttttcatgtc aagacgtcaa cgaagaacct attcaaaaga attcaaacaa
      541 caaatcgtca atctctatct cgctggtaag cctcgcgcag aaattattcg agagtatgag
      601 cttacgcctt cttctttcga taaatggatg aagcaagcac aatcaacggg ctcattcaaa
      661 aaaagagaca acttaacacc agaacaagca gaattgatcg cactaagaaa gaaaaataag
      721 caactcgaaa tggagaatga tattttaaag caagcggcgc tgatattcgg acgaaaagac
      781 aagtaattga tgccaacaag cataaatatt ccatatcagc gatgtgcaaa attctaaata
      841 tttctcgtca gacctactat tatcaa
//
LOCUS       DACDEG010000245          862 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.255, whole genome shotgun sequence.
ACCESSION   DACDEG010000245 DACDEG010000000
VERSION     DACDEG010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.255"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            40..747
                     /locus_tag="IV142_002878"
     CDS             40..747
                     /locus_tag="IV142_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP8486442.1"
                     /translation="MSPGINILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSV
                     SKYQESYKILQTIDQEKVSNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTE
                     TDIHHLQAAANVAMIYLSKGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYR
                     KIEDYDKAIQLCEKGIEYNKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIA
                     RFNGNDYAVKVIENDLK"
BASE COUNT          318 a          124 c          161 g          259 t
ORIGIN      
        1 tgtgaacaag caactgtcag caatatcgaa aacaagaata tgtcgccagg catcaatatc
       61 ttaagtgcgc tttgcaaaaa gctgaatgtc gagatctccg atatattagt agaaacagaa
      121 gaagacaaaa tcaggaaaaa aataaatgag attcaatcat tgaattctgt atcaaaatac
      181 caagaatctt ataaaatttt acaaacgatc gatcaggaaa aagtaagtaa ttctgatctt
      241 ttaaatcaaa ttttatttta tcaaggactg cttgatcatc tttatgcgaa aaaaccagat
      301 gcagcattat tttattttaa tcaagtgttg gaacaaacga ccgaaacaga tatccatcac
      361 ttacaagcag ctgcaaacgt ggcaatgatc tacttgtcaa aaggagaatt agattttgcg
      421 aaagtttatg tggagcgtac acttaaaata ttgagtgaga ctgattttga taatctgatg
      481 gtctgcattg tatattatga tatcgccacc tattaccgta aaatcgagga ttatgataaa
      541 gcaatccaat tatgtgagaa gggtattgag tataataaga aacataaatc gacttatgca
      601 ttggaatact tgctttatga aattgcttct tgccataagc aattaggaga agatgattat
      661 ttagaaagat atatggacgc aaaaaaaata gcgcgtttca acggaaatga ttacgcggta
      721 aaggtgattg aaaacgacct gaaataaagt ggttgtgaaa tcagtgttct gatttgaata
      781 ccgtttattc gaaaaaagga ggtgtgactt ttgtcatacc tccttttttc gatattgttt
      841 gattcctatt taggcaacat at
//
LOCUS       DACDEG010000246          845 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.256, whole genome shotgun sequence.
ACCESSION   DACDEG010000246 DACDEG010000000
VERSION     DACDEG010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.256"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..116)
                     /locus_tag="IV142_002879"
     CDS             complement(<1..116)
                     /locus_tag="IV142_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP8486443.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSS"
     gene            complement(116..>845)
                     /locus_tag="IV142_002880"
     CDS             complement(116..>845)
                     /locus_tag="IV142_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8486444.1"
                     /translation="LDCNIFTEINLLNEYYDEIIEVAKKKDRLLYLSSTFLKRKEIQY
                     IKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNFFVSNKKTNGCREIFAIELPWIIDTF
                     GKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETGIVGTLSVDVVSRVAKRNLTIIGEKI
                     LIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHDNNYSHTIIEDAYLEEIKEFFTLLEK
                     KMEPQYTFEKDKYIINLINDIEVN"
BASE COUNT          287 a          144 c           91 g          323 t
ORIGIN      
        1 ctgctactat aagctgttat atcaaaatta atagaattct tttttccata gcttattaga
       61 ttttttagat gtctctttcc aattgaccct aacccaaaaa aaattacttt catcattaat
      121 ttacctcaat gtcgttaatt aagttaataa tatacttatc tttttcaaaa gtatattggg
      181 gttccatttt cttttccaaa agcgtgaaaa attctttaat ctcctctaaa tatgcatctt
      241 caataatagt atgtgaataa ttattatcat gcataacctc atcgtacaat tgaatatctg
      301 tcatactaga agtttcttca ttccatgaaa atagagaatc cgggttacct tcccattcta
      361 ttaaaatctt ttcaccaatt atagttaaat ttcttttagc tactcttgaa acaacatcta
      421 cacttaaagt tccaactata ccagtttcat gctcaataat aagagtgaaa ctgtccgaat
      481 aatcaatatc aagattggat aatttctggg tcattacagt aaacgattta actttcccaa
      541 atgtatctat aatccaaggt aattcaatag cgaatatttc tctacaccca ttagtttttt
      601 tgtttgaaac aaaaaaattg cgataatctt cccaaggatg ccagtcgggt aaatactgtc
      661 ctacatggta ttgataagaa atttttttat tatgcgtcac tttagactta atatattgaa
      721 tttcttttct cttcaaaaat gtagaagata gataaagtaa tctatctttc tttttagcta
      781 cttcgattat ttcatcataa tattcattta ataaattgat ttccgtaaag atattacaat
      841 ctaaa
//
LOCUS       DACDEG010000247          839 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.257, whole genome shotgun sequence.
ACCESSION   DACDEG010000247 DACDEG010000000
VERSION     DACDEG010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.257"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>839
                     /locus_tag="IV142_002881"
     CDS             <1..>839
                     /locus_tag="IV142_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HAP8486445.1"
                     /translation="TQPITADDTEFVSSATKLDDIIENPGDNIPAGYHKVTFTAGEGT
                     SIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKL
                     DDIIENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYT
                     DAKWPEEATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKN
                     KEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEP
                     TVEG"
BASE COUNT          336 a          139 c          187 g          177 t
ORIGIN      
        1 actcaaccaa ttacagcaga tgatacagaa tttgtatcaa gtgcaacaaa attggatgat
       61 atcattgaaa acccagggga taacattccg gctggctatc ataaagtaac ctttactgca
      121 ggagaaggaa caagtattga aagtggaaca acagtctttg cagtgaaaga cggcgtaagc
      181 ttaccagaag ataaacttcc agtgttgaaa gcaaaagatg gttatacaga tgcgaaatgg
      241 ccagaagaag caactcaacc aattacagca gatgatacag aatttgtatc aagtgcaaca
      301 aaactggatg atatcattga aaatccagga gaaaacattc ctgcaggcta ccacaaagta
      361 accttcaccg caggagaagg aacaagtatt gaaagtggaa caacagtctt tgcagtgaaa
      421 gacggcgtaa gcttaccaga agataaactt ccagtgttga aagcaaaaga tggttataca
      481 gatgcgaaat ggccagagga agcaactcaa ccaattacag cagatgatac agaatttgta
      541 tcaagtgcaa caaaactgga tgataaatct gacgctgaca aatataatcc tgaaggtcaa
      601 aaagtgacta cagaattgaa taaggaacca gacgcatttg agggaattaa gaacaaagaa
      661 gatttaccaa aagatactaa gtatacttgg aaagaaaaag tagatgttag cgcagctgga
      721 aataaaaaag ggactgttgt agtgacatat ccagatggat catctgatga agttgaagta
      781 gatgttacag tgacagacaa tcgttctgac gccgataaat atgagccaac agtagaagg
//
LOCUS       DACDEG010000248          837 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.258, whole genome shotgun sequence.
ACCESSION   DACDEG010000248 DACDEG010000000
VERSION     DACDEG010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.258"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            203..733
                     /locus_tag="IV142_002882"
     CDS             203..733
                     /locus_tag="IV142_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486446.1"
                     /translation="MISDNKWEINLKRFISEYIYTIETKSSFSLNEDSDPLKKYTIQC
                     KKNKDEKLFVIRPDLMKSNTHYIKEKPKDCDYIIISEANKTVFLIELKSSAQTSPAKE
                     ISEQIDGGYKWLQHLSFLTKYDFTEFSYKIIKVCCKFEARQDRKNLFKPNEYGIYNMN
                     GPKLDLRELYRKCNDL"
     gene            complement(728..>837)
                     /locus_tag="IV142_002883"
     CDS             complement(728..>837)
                     /locus_tag="IV142_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8486447.1"
                     /translation="NIVKDLNEGVSISKIAKNYNVTRQTIYRIKKDLNL"
BASE COUNT          335 a           98 c          129 g          275 t
ORIGIN      
        1 catgatgaat ggatttattc ttcaagaaaa tacatcaatt ttgataagta gaaatggtaa
       61 aaacattgta tagcatttta cacaggagtc tggacttgac taaaatttct aacatcagtt
      121 attatgataa ttttgatgat gtcaattatg atttacagga aagatataat gatttaatcg
      181 aagaattaga ggagagagga gaatgatttc agataataaa tgggaaataa atctcaaaag
      241 atttatttca gaatatatat atacgattga aactaaatct agtttttctc ttaatgaaga
      301 cagtgatcct ctaaaaaaat acactattca atgtaagaaa aataaagatg aaaaattgtt
      361 cgtaataaga ccagatctta tgaagagtaa tacccactat ataaaagaaa aacctaaaga
      421 ttgtgactac atcatcataa gtgaagcaaa taaaacagta tttctgatag aacttaaaag
      481 ctcagctcag acttcccctg caaaagaaat tagtgaacaa attgatggtg gatataagtg
      541 gttacaacat cttagttttc taacaaaata tgattttact gaattcagtt ataaaattat
      601 taaggtatgt tgtaaatttg aggcaagaca agatcgtaaa aatcttttta aacctaatga
      661 atatggtatt tataatatga atggtccaaa attagactta agagagttgt atagaaagtg
      721 taacgatcta taaattcaaa tcttttttaa ttctatatat tgtttgtcta gtaacgttat
      781 aattctttgc aatctttgaa atagagacgc cctcattcaa gtccttaacg atgtttt
//
LOCUS       DACDEG010000249          793 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.259, whole genome shotgun sequence.
ACCESSION   DACDEG010000249 DACDEG010000000
VERSION     DACDEG010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.259"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>793
                     /locus_tag="IV142_002884"
     CDS             <1..>793
                     /locus_tag="IV142_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HAP8486448.1"
                     /translation="VIDASKHLYPISAMCRILGLSRQSYYYQSKPKKDESELEEVVAE
                     EFIRSRKAYGSRKIKKALSKRGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEA
                     KTTNVLNRKFLRDNPLEAIVTDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAV
                     LVKKAFSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAE
                     STYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYET"
BASE COUNT          279 a          140 c          151 g          223 t
ORIGIN      
        1 gtaatcgatg cgagtaagca tctttaccct atatcagcga tgtgcagaat attaggtcta
       61 tcacgtcagt cctattatta tcaatcaaaa ccaaagaaag acgaatcaga acttgaagaa
      121 gtagtcgctg aagaatttat ccgcagccga aaggcctacg gctcaagaaa aataaaaaaa
      181 gccttatcaa aacgaggcat tcagatcagc cgacgaaaaa ttagtagaat catgaaaaat
      241 agaggattaa aatcgagcta tactgttgct tattttaaag tacatcattc tacttgcaat
      301 gaagccaaaa cgacaaacgt attgaatcgt aaattcttaa gagacaaccc attagaagcg
      361 atcgtaacag acttgactta tgtacgagtc gggaaaaaat ggaattatgt ctgtttcatt
      421 ttggatctgt tcaatcgaga aattctcggc tattcttgtg gagaacataa agatgccgtt
      481 ctagtaaaaa aagcatttag ccgtatcaaa caacctctga cagaggttga gatttttcat
      541 actgatcgtg gaaaagagtt tgataaccaa gctattgatg aattattaac aacttttgac
      601 atcaatcgat cattgagtca taaaggctgt ccttttgata atgccgtagc tgaatcaact
      661 tataagtcgt tgaaagtaga atttgtctat caatacacat ttgaaacctt acaacaattg
      721 gatttggagt tatttgacta tgtcaattgg tggaaccacc ttcggttgca cggtacactt
      781 ggctacgaga cac
//
LOCUS       DACDEG010000250          783 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.260, whole genome shotgun sequence.
ACCESSION   DACDEG010000250 DACDEG010000000
VERSION     DACDEG010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.260"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(7..>783)
                     /locus_tag="IV142_002885"
     CDS             complement(7..>783)
                     /locus_tag="IV142_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP8486449.1"
                     /translation="IFYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKI
                     KWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQH
                     SSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELK
                     RAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFIL
                     SIFIDEMVEAHVLFNKRDVISIVFWYYLGSISYLYLKKEN"
BASE COUNT          300 a          138 c           93 g          252 t
ORIGIN      
        1 ttcatattaa ttctcctttt ttaaatacaa gtaagaaatg cttcccaaat aataccagaa
       61 aacaattgaa atgacatcgc gcttattgaa aagtacatga gcctccacca tttcatctat
      121 aaaaatagat agtataaaaa ttaaaacaat accaagaaac gcataattca ctttacttct
      181 aaaaaatgtt ttgagtaaac taaagatatt taacacatag aaagcataga tacatataaa
      241 agcaattata ccacttgata caagtacttg aagaaatcca ttatgcatgt ttccatgtgc
      301 tctttttagt tcattcacat ctgtagtatc aagatctact tttttcaaaa attttgcatc
      361 gctattccca tagaaatcgg tctctccata accaaataac aaagttggtc ttataacttt
      421 aagcgcagct ttccaaatcg taaaacgacc agctgaaatt tcggagttat cactagaatg
      481 ctgtaatatt acttcttttt ctggaagtgg agttttatct gattcatttg actctataac
      541 ttctactttt ctatcaatcg atacaggaat tgttgctaaa aaatccttag cataattagt
      601 accaaaagaa aaatatacca atagaactaa taatatgctt accatccatt tgatttttac
      661 aggtagtact tctctaatgc tctttctaaa ttttgaattg gcaaacaaca aaagaaataa
      721 tactaaaaaa gaacaaatta ctacctgggc acctctagac gaagaaagtg taatataaaa
      781 tat
//
LOCUS       DACDEG010000251          776 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.261, whole genome shotgun sequence.
ACCESSION   DACDEG010000251 DACDEG010000000
VERSION     DACDEG010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.261"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..270)
                     /locus_tag="IV142_002886"
     CDS             complement(<1..270)
                     /locus_tag="IV142_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8486450.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTI"
     gene            complement(342..500)
                     /locus_tag="IV142_002887"
     CDS             complement(342..500)
                     /locus_tag="IV142_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8486451.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
BASE COUNT          227 a          133 c          112 g          304 t
ORIGIN      
        1 aattgtttga cctaattcat atctaggaag ccatttagat tcgtaaaatt ttttaaacgt
       61 tatttttcct tttaattcta atgagtttgc attctcctct ctcaaaactt tttctatttt
      121 cttcttcata tcattttcgg ctttaatcgc caattgccta tttggaaatg ttttttgata
      181 cttttctgat gcaattcctg ttatttcttt aaaccttttt ggatatctca gattagtttt
      241 atatttccca tttgaaagct ttgtaggcaa aatcctcact ccttaaaaaa agtaaggcag
      301 tatatttcac taattttagt atatgcggcg agaagaaaat ttcaagttat tttaaatgta
      361 ttcataaagt ctttaatatc ttctacgtca aagcgaacta caccaccaac tcttattact
      421 gggaaattat atatacgaat ccacttatct agagtattat tatcgattcc tagaaatgca
      481 caggtttgtt ttttattcag gtagactctg gattctataa aatcatctcc taaaatagta
      541 gttatgaaat ttttgaattg ctttgggtaa gcacatagga atttcacctc tccccagttt
      601 acggcgagcc ttacggaagt atcattattt gctgctattg tcatggcatt ttgttttccc
      661 agaacaaaaa gaaatctcta agtatttgtc gctaatcagg tcatggcgta cttgagtttc
      721 tagctcgaat agcagggatc gttattcaaa aattgtattt aattgtcaat gatcaa
//
LOCUS       DACDEG010000252          748 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.262, whole genome shotgun sequence.
ACCESSION   DACDEG010000252 DACDEG010000000
VERSION     DACDEG010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.262"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            554..>748
                     /locus_tag="IV142_002888"
     CDS             554..>748
                     /locus_tag="IV142_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8486452.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYY"
BASE COUNT          271 a          136 c          115 g          226 t
ORIGIN      
        1 gacactcctc ttaatataca gtcctattgt ataaagtgtt actatataca tagtaactgt
       61 atacataaaa aaagtcaagt attagaaaaa agttcaaaag atcaagtgtg cgcttcgctc
      121 ccactcccct cgacgtttta gtagcctttc cctcacttcg ttcagtccaa gccaactaaa
      181 aagttttcgg gatacattct ccttctcttc ctaataatca attaaaattt taatctttat
      241 atttctgcta atcactcagg aaacagtaca gaaaaacaaa cacgtatcat agatataaat
      301 gtaatggcat agtgcgggtt ttattttcag cctgtatcat agctaaacaa atcgagttgt
      361 gtgtccgttt ttgggcgttc tgctagcttg tttaaagtct cttgaatgaa tgtatgttct
      421 aagtcaaaaa actctgacag cgcctttata tagataaagt ccgtataatt gtgtaaaagt
      481 aaaaaggcca tataacagtc cttttacggt acaatgtttt taacgacaaa aacataccca
      541 ggaggacttt tacatgaccc aagtacattt tacactgaaa agcgaagaga ttcaaagcat
      601 tattgaatat tctgtaaagg atgacgtttc taaaaatatt ttaacaacgg tatttaatca
      661 actaatggaa aatcaacgaa cagaatatat tcaagcaaaa gaatatgaac gaacagaaaa
      721 ccgacaaagt caacgaaatg gctattat
//
LOCUS       DACDEG010000253          736 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.263, whole genome shotgun sequence.
ACCESSION   DACDEG010000253 DACDEG010000000
VERSION     DACDEG010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.263"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            50..721
                     /locus_tag="IV142_002889"
     CDS             50..721
                     /locus_tag="IV142_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8486453.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
BASE COUNT          287 a          112 c          120 g          217 t
ORIGIN      
        1 tagaactaaa atatcaaatt caaaaatgaa attttgagag ggattaaata tgacaattaa
       61 aactagtctc caaaatcaag cttatcaatc tattcgtcag caaattatct acgctgactt
      121 agaacctgga aaaaaagtat cagaaaaaaa attggaagaa acgctttcta tcggtcgtac
      181 tccaatacgt gaagcattga ttcaactccg tcatcaagga cttgttgata caattcctca
      241 aagtggaaat tatatctcat taatcgactt agatttagca aaaaatgctc gttttgtcag
      301 agaacattta gaacgacaaa ttatgctaga gtgttgtgcc aagatgaata atcaaaagaa
      361 acaagtttta gaaactataa ttgcagaaca agaaaaagct gttagtcaaa agaatgctcg
      421 tagttttttt cataatgata atttatttca taaagcttgt tttgaaattg cagaacggga
      481 agaagtatgg caatggttgg aagataataa tacgcacttg gaacgatttc gttggttacg
      541 agtaaaaacg gaaggattaa aatgggaaaa aattatggaa caacatcatc aactatttaa
      601 agccttgacc atgcaagacc ctgaagaagc taacttctta acatccttgc atcttcattt
      661 aatgcttgat gaacaaaatg ttgtattgac tactcaccca aaatatttta aaaatatata
      721 actgctaaat ttcttt
//
LOCUS       DACDEG010000254          699 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.264, whole genome shotgun sequence.
ACCESSION   DACDEG010000254 DACDEG010000000
VERSION     DACDEG010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.264"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(35..>699)
                     /locus_tag="IV142_002890"
     CDS             complement(35..>699)
                     /locus_tag="IV142_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8486454.1"
                     /translation="KLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGS
                     LLDPFLIQNMQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRK
                     DYKDFAKLSVTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLIN
                     HVVVQIPWTTPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSEN
                     ET"
BASE COUNT          240 a          122 c          111 g          226 t
ORIGIN      
        1 cttcttgtgt taatttttgt tatctcatag taaattatgt ttcattttca cttaatttag
       61 ttgtcacgtg ttcgtcaatt cgtaaaaacg gtaaatatat aaacacactt gcggctataa
      121 ttaatatttg taaaacagct gctcgccagt ctccggctgt tgctaaaaat cccgatataa
      181 ctggtggcgt agtccaagga atttgcacta ctacatgatt aattaatccc acagcagttg
      241 caaaatatgc tactgtcaaa gaaaaaatcg gtgcaagcac aaagggaata atcagactga
      301 tattaaatac aataggcaat ccaaaaataa taggttcgct tatattgaat atagcgggag
      361 ttaccgataa tttagcaaag tccttataat cttttctacg actgaagata aatattgcaa
      421 tcaataaagc aatcgtattt cccgatccac ccattaccgc aaaggtatct ttaaaggcca
      481 tattgataat atgtggaatt tgctctttgt ttgcataagc tgccatattt tcttgcatat
      541 tctgaatcaa aaacggatct aacaatgaac ctgatattac tgcttgatgg ataccaaatc
      601 caaaaaatag attagcaata gatgtaatta acaaaaatcc tggtaaacca gttgtaattt
      661 tacttaatgg ctctgtaact atcgtggtaa ttagcttat
//
LOCUS       DACDEG010000255          689 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.265, whole genome shotgun sequence.
ACCESSION   DACDEG010000255 DACDEG010000000
VERSION     DACDEG010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.265"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            82..>689
                     /locus_tag="IV142_002891"
     CDS             82..>689
                     /locus_tag="IV142_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAP8486455.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRK"
BASE COUNT          230 a          119 c          135 g          205 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa
      661 ttaattgatg tcttgcctac gcgtaaatt
//
LOCUS       DACDEG010000256          655 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.266, whole genome shotgun sequence.
ACCESSION   DACDEG010000256 DACDEG010000000
VERSION     DACDEG010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.266"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..189
                     /locus_tag="IV142_002892"
     CDS             <1..189
                     /locus_tag="IV142_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8486456.1"
                     /translation="NSNGILRRNGLPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
BASE COUNT          219 a          125 c           76 g          235 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatagattt tagagaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgcaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcatata atttaaaatt aacattgatt ctcctatgaa aatatctttt
      241 acctttctcc tgtgaaaagg aaatttcctt ttttatagtg ttattgatat aatcagaata
      301 ttcatcaaac tctgtatctg gagttaaaaa tctacagtaa catctataag ctttgctagt
      361 aatcttcttg ggttctctct ccattgtcaa gcaacatcaa aacacctact tactattcga
      421 ttcttttctg ccaaaaacga gtcagcatca ctcacaactt atgtgttgct gactccgact
      481 agtcctttgt tcaatatcaa cttattaaac agatataata tttttcttta tttcgtatac
      541 tccaagactt ccatctttct ttcatcttat ctctgaaaaa aacacagcaa agtgccctta
      601 ataaatatac attagaattt gcaaattttt tcatcttaag atatatccta ttcat
//
LOCUS       DACDEG010000257          626 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.268, whole genome shotgun sequence.
ACCESSION   DACDEG010000257 DACDEG010000000
VERSION     DACDEG010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.268"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..129
                     /locus_tag="IV142_002893"
     CDS             <1..129
                     /locus_tag="IV142_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HAP8486457.1"
                     /translation="ESQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            259..>626
                     /locus_tag="IV142_002894"
     CDS             259..>626
                     /locus_tag="IV142_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAP8486458.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSA"
BASE COUNT          270 a           88 c           96 g          172 t
ORIGIN      
        1 gaatctcagt ttaatctcga aaaatttatg gagtattatt ctattcagaa taagctcaga
       61 tacacttgtt atgctgtctg ccaatgctat tttaagaaaa aatattctta cgcaaggcaa
      121 tatgtatgaa taaatcaaga attattaaaa aaaccgattt cagcaaaaca ttaaaaaagc
      181 ccgtgctgca acacgggctc tttcctcata tatgagcttc tacaaaaaaa atcatatcac
      241 agaaatgagg aagaaaaaat ggaaaaagag attttaataa aatctccagg aaaaaccttg
      301 attaaagtaa ctgatgattc tatttcaatt attagaaaag gatttataaa tttagttaat
      361 caaggtatta aaggagaaaa aactatcccc tttaaaaata tatcagccgt acaattaaaa
      421 aaaccaggta tgagtaatgg ttatatacaa tttaccttgc taggcggaaa cgaaagtcgc
      481 ggaggtatat tagcggccac gaaagatgaa aacaccgtta tgtttacaaa aaaatattgg
      541 aatgaaatgg aaaatttaaa aaaatatata gagaagcaac aatctatatt agataataat
      601 tccaaagaaa cacaaatttc aagtgc
//
LOCUS       DACDEG010000258          596 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.269, whole genome shotgun sequence.
ACCESSION   DACDEG010000258 DACDEG010000000
VERSION     DACDEG010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.269"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            380..>596
                     /locus_tag="IV142_002895"
     CDS             380..>596
                     /locus_tag="IV142_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486459.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHY"
BASE COUNT          235 a           69 c          113 g          179 t
ORIGIN      
        1 ggtagaaggt gatcaaaatg ggagtaaacg aaatgatgaa agtcagattg agtttttaaa
       61 tgataccttc ctcagaactt caaaacgaat tcaaagattc gttgacaaat cttgagcaag
      121 atcatttaaa aaaatatttt ctcttcattt gacgaaaaat gtttagaaat cttatataag
      181 tgaagataaa gtaattggtg taattaattg tagagaatag cctttgagca attgaagcga
      241 tacaattatt tagaaaatga acttcctcca gcatactaca tgattcctta cgggacgatg
      301 ttaaagaaga aagcaagaaa ttttagaagt attagtagat aaactaatgg gcttttctca
      361 atttaaggga gtagcccaaa tgtttatcaa atatggtgca gatacagtag caaccatgtt
      421 tacagaagaa tatcaagtag actattatga agtaatagat aaatgtgtac attttgttta
      481 tgaaaaagtg tggaatttta tgaattttgc gataagtgaa gaaatcttac gagtacaaga
      541 aaacagaaca ggaatattta ccagagacag aaagcttaat ttgattgcgc attata
//
LOCUS       DACDEG010000259          593 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.270, whole genome shotgun sequence.
ACCESSION   DACDEG010000259 DACDEG010000000
VERSION     DACDEG010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.270"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(318..581)
                     /locus_tag="IV142_002896"
     CDS             complement(318..581)
                     /locus_tag="IV142_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circular bacteriocin, circularin A/uberolysin
                     family"
                     /protein_id="HAP8486460.1"
                     /translation="MKKNLLLVLPILGFAGFFVGVPMLSANIGISSYAAKKVIDIINT
                     GSTVATIISIVAAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
BASE COUNT          224 a          104 c           79 g          186 t
ORIGIN      
        1 gccttccttt tttgtatgga ttttttcatt tacacaaaat attttatact ctcctgaatt
       61 ttttcatgtt gaaaacatca ctaatcaaag gcaaattata atttagaaat agaatgaaaa
      121 cactaaatga aaatagaaca ttagaaaaaa tagaatcaaa aaaacccaag actagtgccg
      181 aaataaggga aacaatcgaa gctacaaaaa ataatgattt cgttgaacag gaccaattta
      241 tttgtttccg atatttctca aaaagaactt tctttgggaa agttaaaggc gactgtaaag
      301 tcgcctttag tcctttttta ccaagctgct gcatatttag ctccatattt tttaattaat
      361 gatttggctg tagcaacaat accagctgta attaatccgc ctccaacaac tgcagcaaca
      421 atagaaatga ttgtagcaac cgtgctacca gtattaatta tgtcaataac ttttttagct
      481 gcataagaag aaattccaat gttagcactt agcattggta caccaacaaa gaatcccgca
      541 aaacctaaaa ttggtaaaac taataataaa ttctttttca taataattcc tct
//
LOCUS       DACDEG010000260          579 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.271, whole genome shotgun sequence.
ACCESSION   DACDEG010000260 DACDEG010000000
VERSION     DACDEG010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.271"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..435)
                     /locus_tag="IV142_002897"
     CDS             complement(<1..435)
                     /locus_tag="IV142_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8486461.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYVSVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFE"
BASE COUNT          176 a          104 c           77 g          222 t
ORIGIN      
        1 ttcaaaaaaa atttgatatt tttttagatt tctttttagt ctttgtaata ctttataagc
       61 cacagaataa ctcacgtgat gctgtttagc aaaattttct actgagacat atttttcctg
      121 aaagaaagcc aataccattt tcatagataa agaatccgtt atataacgag ataacagagc
      181 tttgctagaa cattttccat taatttccag acatatatcc aattcactga cggttactcg
      241 caaatttttc tctagatcga acttttgtaa atcatgtttc agcttatcga tcgttttttt
      301 caagagaaat gtcgatagcg acaactcttc ttgaacattc gatattgact ctgataaatt
      361 tgagcttgct tctaaatatt taagtaatga ataacttctg tgatcttctt tgcttaaaaa
      421 aaactcataa ttcattagtc atcagctctt tctacgtata ttactttcca tgcattcacc
      481 aactagctgg ggataaaact gatttcctgg atccgagtca ctgttgtcag cgattcttgt
      541 ttgtataata tacatcgtcc tgtctttttt ttaacattt
//
LOCUS       DACDEG010000261          575 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.272, whole genome shotgun sequence.
ACCESSION   DACDEG010000261 DACDEG010000000
VERSION     DACDEG010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.272"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            312..>575
                     /locus_tag="IV142_002898"
     CDS             312..>575
                     /locus_tag="IV142_002898"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP8486462.1"
                     /translation="MSAVVTDLTYVRVGTNWAYICLMIDLFNREIIGHSCGNKKDAQL
                     VKRAIQSIPYSLQEIELFHTDRGKEFDNQTIQNLMNGLVIKFLS"
BASE COUNT          195 a          105 c           82 g          193 t
ORIGIN      
        1 ttttacactc tctattattc ttttcataaa tattgtaaat agaagtatta aaaatatata
       61 caaaaagtat tatttcgaga aagataaata ttttcaaaaa ccttttcata atcctcaccc
      121 tttcttacac tgataaatca tgcgtctaat attcaaatta tactcgatat gctttagcta
      181 atcagccaac tctgtttgtc tactatcatt atatcaatag acaatatctg ctcccgtctt
      241 tatgatcgtg ccaaagcaat caaaagaaaa ttgcgaatca cttagatcga aagtttactc
      301 aaacaaaagc attgtcagcg gttgtcacgg atttaactta tgttcgggtc gggacaaatt
      361 gggcttatat ttgtttgatg attgatttat tcaatcgtga aattattggc cattcttgcg
      421 gaaataaaaa agatgcgcag cttgtaaaaa gggccattca aagcattcct tactcacttc
      481 aagaaatcga actcttccat acggatcgtg gaaaagaatt tgataaccaa accattcaga
      541 atctcatgaa tggtttggtt atcaaattcc tttcc
//
LOCUS       DACDEG010000262          565 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.273, whole genome shotgun sequence.
ACCESSION   DACDEG010000262 DACDEG010000000
VERSION     DACDEG010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.273"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..561
                     /locus_tag="IV142_002899"
     CDS             <1..561
                     /locus_tag="IV142_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8486463.1"
                     /translation="QGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTY
                     AGMKVFQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFI
                     FLDEPFVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAH
                     GSVESVFNEENLKKAYGDTIFIGEGE"
BASE COUNT          220 a           58 c          117 g          170 t
ORIGIN      
        1 cagggagagg tctccattaa taatgaagat atcaataaaa aactggtgaa gattgcatat
       61 gtagagcaaa aagctgatat tgattttact tttccaataa aagtaaaaga atgtgtatct
      121 atgggaacat atgccggaat gaaagttttt caaagaatta aaaatgcaga atggcaaaga
      181 gtttcaaagg ctttggaaaa ggtcgatatg ggaaaatatt ctaatcatca aataggagaa
      241 ttatctggag gacaatttca acgtgtactt ttagcaagat gtcttgctca aaatgcggat
      301 ttcatatttt tagatgaacc atttgtaggg atagacttag ttagtgaacg aataattatg
      361 gatacgttaa aaaaattgaa acatcaggga aaaacagtgc taattgtgca tcatgattta
      421 agcaaggtaa agaagtattt tgataatatt attcttttga atcgaaatct aattgctcac
      481 ggaagtgtag aatctgtatt taatgaagaa aatttaaaaa aagcctatgg agatactata
      541 tttattggag agggggaata atatg
//
LOCUS       DACDEG010000263          554 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.274, whole genome shotgun sequence.
ACCESSION   DACDEG010000263 DACDEG010000000
VERSION     DACDEG010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.274"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(31..116)
                     /locus_tag="IV142_002900"
     tRNA            complement(31..116)
                     /locus_tag="IV142_002900"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(80..82),aa:Leu,seq:taa)
     gene            complement(133..204)
                     /locus_tag="IV142_002901"
     tRNA            complement(133..204)
                     /locus_tag="IV142_002901"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(170..172),aa:Gly,seq:gcc)
     gene            complement(216..288)
                     /locus_tag="IV142_002902"
     tRNA            complement(216..288)
                     /locus_tag="IV142_002902"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(253..255),aa:Thr,seq:tgt)
     gene            complement(306..387)
                     /locus_tag="IV142_002903"
     tRNA            complement(306..387)
                     /locus_tag="IV142_002903"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(351..353),aa:Leu,seq:tag)
     gene            complement(395..467)
                     /locus_tag="IV142_002904"
     tRNA            complement(395..467)
                     /locus_tag="IV142_002904"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(432..434),aa:Lys,seq:ttt)
     gene            complement(471..543)
                     /locus_tag="IV142_002905"
     tRNA            complement(471..543)
                     /locus_tag="IV142_002905"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(508..510),aa:Val,seq:tac)
BASE COUNT          140 a          157 c          135 g          122 t
ORIGIN      
        1 tccagttgag ctatgggcgc atatttgaaa tgccgaggac cggaatcgaa ccggtacggt
       61 gatcactcac cgcaggattt taagtcctgt gcgtctgcca gttccgccac cccggcgcga
      121 ttgctttggc aaagcggaaa acggggttcg aacccgcgac ccccaccttg gcaaggtggt
      181 gctctaccac tgagctattt ccgcgtgttt aaagatgccg gctaaaggac ttgaaccctc
      241 gaccctctga ttacaaatca gatgctctac caactgagct aagccggcat gtaaaactta
      301 aattatgcgg gtgaagggac ttgaaccccc acgccgtaag gcgctagatc ctaaatctag
      361 tgcgtctgcc aattccgcca cacccgcaaa aatatgagcc gtacagggct cgaacctgtg
      421 accctctgat taaaagtcag atgctctacc aactgagcta acggctcata tggaggttaa
      481 cgggatcgaa ccgctgaccc tctgcttgta aggcagatgc tctcccagct gagctaaacc
      541 tccatggata actt
//
LOCUS       DACDEG010000264          534 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.275, whole genome shotgun sequence.
ACCESSION   DACDEG010000264 DACDEG010000000
VERSION     DACDEG010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.275"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(67..288)
                     /locus_tag="IV142_002906"
     CDS             complement(67..288)
                     /locus_tag="IV142_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8486464.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
BASE COUNT          185 a           88 c          102 g          159 t
ORIGIN      
        1 atttttttgt tttcattaag acgtattacc taagagttcc atgactttag tctggtagcg
       61 tgtcagctaa ccaaaaaaga gaaaaagaaa agtaaaaaat tcgactttct tttttctata
      121 gtatataaat ttttgcatat agataaagat caagataccg ataacggaag ctagattgta
      181 atctatttta gtgacagtca ggtatagcca agtaactaga agatcagtgg aacctgcacc
      241 ataaaagttt gagtttgtcg gtccgttgac agttaaaaga aaaatcacgt tgaagttatt
      301 gatatttccg attacgccgc ttcgatttgg tcacgatcca aattttggat aatccctgta
      361 gaaattagca tagtagctgg gataccaatt cacatattga caaagataac agagactttt
      421 acataaaacg gatcaatcag gaaaggaatt gctcgatcca gccccagttt tccaacaatg
      481 tgttaactgg cctagcgtca ttcagcaaat tatgcatgat gaggagcgaa atga
//
LOCUS       DACDEG010000265          506 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.277, whole genome shotgun sequence.
ACCESSION   DACDEG010000265 DACDEG010000000
VERSION     DACDEG010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.277"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(426..499)
                     /locus_tag="IV142_002907"
     tRNA            complement(426..499)
                     /locus_tag="IV142_002907"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(463..465),aa:Asn,seq:gtt)
BASE COUNT          163 a          100 c          104 g          139 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa tgagaaaaag catggaatgc gctgcatcag gtatcgaggc ttagaaaaaa
      241 atcaggtgca tacgatgctt gtttttgccg aaatgagtct gaaaaaattg gtcacttagc
      301 agtgggagca tatgtcttca tattttttat tatacaaaaa tagacaaaag tatcagtcta
      361 cactcttagt aaagaggaaa ctgatacttt tgtctacagt atgatatttc ttacatctac
      421 ttttactccg gcagtaggac tcgaacctac gacatcatga ttaacagtca tgcgctacta
      481 ccaactgagc tatgccggaa taacat
//
LOCUS       DACDEG010000266          505 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.278, whole genome shotgun sequence.
ACCESSION   DACDEG010000266 DACDEG010000000
VERSION     DACDEG010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.278"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            71..>505
                     /locus_tag="IV142_002908"
     CDS             71..>505
                     /locus_tag="IV142_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486465.1"
                     /translation="MFNLDKIITWRENWSKNILGELEVGRVYTNKEISEKFKCSKQGG
                     MRRSHQTNTLVLFSDQTGSNVYKDKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSN
                     FVKIHLFETFKPKEHTYLGEVYLAGQIYTVNEKDSSGNSRKVY"
BASE COUNT          208 a           50 c           99 g          148 t
ORIGIN      
        1 aaaaaaatta gatatttcta ctggtgtatt agctaattta attagaaatg gcatgccaaa
       61 agagggagaa atgtttaatc ttgataagat tattacatgg cgagaaaatt ggtctaaaaa
      121 catattaggt gagttagaag ttggtagagt atatactaac aaagaaattt cagagaagtt
      181 taaatgttcc aaacagggtg gaatgagaag atctcatcaa actaatactc ttgtactgtt
      241 tagtgatcaa acaggaagta atgtttataa agataagtgg ttaaatggta ttttacaata
      301 taccggtatg gggctaaaag gggatcaagt attggataag aatcaaaata aagttttagc
      361 caattcaaaa agtaattttg taaagattca tttatttgaa acatttaaac caaaagaaca
      421 cacatatctg ggagaagtat atttagcagg acaaatatat actgttaatg aaaaagatag
      481 ttcgggcaac agcagaaaag tatat
//
LOCUS       DACDEG010000267          492 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.279, whole genome shotgun sequence.
ACCESSION   DACDEG010000267 DACDEG010000000
VERSION     DACDEG010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.279"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>492
                     /locus_tag="IV142_002909"
     CDS             <1..>492
                     /locus_tag="IV142_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8486466.1"
                     /translation="IIKHMNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLKNRA
                     NINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKDPDL
                     FFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNGKIEAKNTHIKTMKRVSY
                     GFKS"
BASE COUNT          175 a          100 c           92 g          125 t
ORIGIN      
        1 attatcaaac acatgaatca agcctttaat gagttgcgta tccgtgaaat gaatgaactt
       61 cgtaaagtag gacagaaaag tcaggcagaa aaactgaaaa agaactggcg ctttttgcta
      121 aaaaatcgtg caaacatcaa ccattatgaa tacaaaacat ggaaaagttt tcgagcacca
      181 aaatacccat ttcttacgga agcgatgatg attgatcgat tgcttggctt ttctacgtcc
      241 ctgaaggagg cgtatcccta ttttcatgaa ttagttgaag cctttcgaga caaagaccct
      301 gacttatttt tctccttatt ggcagaactt cccgaaacgt tagatgacgg ctttcgggaa
      361 aagctccaaa accttctgac ctatgaagaa ggcatcacca acgcgatgat ctatccttac
      421 accaatggaa aaatagaagc gaagaatacc catataaaga caatgaaacg agtatcctac
      481 ggatttaaat ca
//
LOCUS       DACDEG010000268          487 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.280, whole genome shotgun sequence.
ACCESSION   DACDEG010000268 DACDEG010000000
VERSION     DACDEG010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.280"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          161 a           96 c           56 g          174 t
ORIGIN      
        1 ctaaatcact ctacaataac ggcgaaagca caaaaaataa aaaaattatt acgttattaa
       61 aaaaacagat gaaagatcag gttttattag atctcattct ttcatcactt gaagggattt
      121 aatgaactac tttttcctct ttgtcgtttt tatatattcg tattccttca aacagtagga
      181 tagcctcttt caatgcttga tatttgaact gtccgttact taaaaattta gatgtgccgc
      241 cctcttccta gaacatattc ttttttactt tttcattaat tgacagacca atcgaacgaa
      301 actgtaaatt tcgataggat aatgattggt caaactcatt cattttcaag acatctcgat
      361 tcatattcaa gataggaatc tttttatcaa catcacttta ttcgtatttt cctctaccat
      421 aaaagcttca cgctccacaa aattgtttta tacactacta ttcagcccat tcaacgacca
      481 atgaatt
//
LOCUS       DACDEG010000269          480 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.281, whole genome shotgun sequence.
ACCESSION   DACDEG010000269 DACDEG010000000
VERSION     DACDEG010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.281"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..387
                     /locus_tag="IV142_002910"
     CDS             <1..387
                     /locus_tag="IV142_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8486467.1"
                     /translation="RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGK
                     CAITLEEFQQANEIHCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIAL
                     LKLNKVQLIRLNKYRVLAGNQELNLI"
BASE COUNT          186 a           77 c           88 g          129 t
ORIGIN      
        1 agaaaattta tccacaccaa tctaagtgtt gataaattac tgatgtacca gttaatgaaa
       61 ttatctttgg aaaatcgcac aatagaatac gcagacaatc gaatttcgct attttctgct
      121 caacatggaa aatgtgctat aactctggaa gaatttcaac aagctaatga aatacattgt
      181 catcatattg taccaactgg agctggtgga aatgacgatt ataaaaatct tattttggta
      241 aaagaggcgg tacaccgtct aatacacgct aaaacagaag aaacaataca aaaatatata
      301 gctctattga agctaaataa agtccagctc ataagactaa ataaatatag agtattagct
      361 ggcaatcaag aattaaactt gatataggtc tactacataa atacttttca atataaacac
      421 gatggaacgc ggagtgcggt gaaagtcgca cgctccgtgt gaatgggggg aaaagtttga
//
LOCUS       DACDEG010000270          426 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.denovo.283, whole genome shotgun sequence.
ACCESSION   DACDEG010000270 DACDEG010000000
VERSION     DACDEG010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.denovo.283"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            33..>426
                     /locus_tag="IV142_002911"
     CDS             33..>426
                     /locus_tag="IV142_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8486468.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTP"
BASE COUNT          158 a           79 c           70 g          119 t
ORIGIN      
        1 gacaaccaat tcacagaaaa gaggacttcc ctatgaatga ttttactaca gaaattgtgc
       61 aaactctagt cactaaaggc gatttaaatg aattattccg ttcgcactta gaaaaagcga
      121 taaacacact cctacggact gaattaacgg cttttttaga ttacgaaaaa tatgatcgca
      181 ctggttttaa ttcaggtaat tcgagaaacg gttcttactt tcgatcaatc aaaaccgaat
      241 atggtgaatt aacattggaa atacctagag atcgtaatgg tgagtttaaa caacaaactt
      301 taccagccta caaaagaaca aacgatacat tggaaaccac tattatccat ttattcgaaa
      361 aaggtgttac gatgtctgaa attgctgatt tgatcgaaaa aatgtacggt catcactata
      421 ctccac
//
LOCUS       DACDEG010000271        23489 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.guided.033, whole genome shotgun sequence.
ACCESSION   DACDEG010000271 DACDEG010000000
VERSION     DACDEG010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.guided.033"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1276
                     /locus_tag="IV142_002912"
     CDS             <1..1276
                     /locus_tag="IV142_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP8486469.1"
                     /translation="AQKHNCPLVRLDYQQEQGLASSLPLGVNQIKIQRSLTTSSVAVF
                     VPFVTQELFQGGAAMYYGINAKSRNMIMLDRKQARCPNALKLGTPGSGKSMSCKSEIV
                     SVFLTTPDDIFISDPEAEYYPLVKRLHGQVIRLSPTSKDFVNPLDINLNYSEDDNPLA
                     LKSDFVLSFCELVMGGKNGLEAIEKTVIDRAVRVIYRPYLADPRPENMPILSDLHKAL
                     LDQHVPEADRVAQALDLYVSGSLNVFNHRTNVDIGNRLVSFDIKELGKQLKKLGMLIV
                     QDQIWGRVTANRSQGKATWYFADEFHLLLKEEQTAAYSAEIWKRFRKWGGIPTGATQN
                     VKDLLSSPEIENILENSDFITLLNQASGDRKILAERLNLSTEQQKYIDNSEPGEGLLI
                     FENVVLPFTNPIPHNTQLYKIMTTRLNEVAGV"
     gene            1308..3299
                     /locus_tag="IV142_002913"
     CDS             1308..3299
                     /locus_tag="IV142_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAP8486470.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            3322..3573
                     /locus_tag="IV142_002914"
     CDS             3322..3573
                     /locus_tag="IV142_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HAP8486471.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            3563..4792
                     /locus_tag="IV142_002915"
     CDS             3563..4792
                     /locus_tag="IV142_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HAP8486472.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            4789..6870
                     /gene="topB"
                     /locus_tag="IV142_002916"
     CDS             4789..6870
                     /gene="topB"
                     /locus_tag="IV142_002916"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8486473.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            7019..10939
                     /locus_tag="IV142_002917"
     CDS             7019..10939
                     /locus_tag="IV142_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HAP8486474.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            10940..11884
                     /locus_tag="IV142_002918"
     CDS             10940..11884
                     /locus_tag="IV142_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486475.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            11888..12091
                     /locus_tag="IV142_002919"
     CDS             11888..12091
                     /locus_tag="IV142_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486476.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(12391..12627)
                     /locus_tag="IV142_002920"
     CDS             complement(12391..12627)
                     /locus_tag="IV142_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8486477.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(12810..13181)
                     /locus_tag="IV142_002921"
     CDS             complement(12810..13181)
                     /locus_tag="IV142_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486478.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(13178..14506)
                     /locus_tag="IV142_002922"
     CDS             complement(13178..14506)
                     /locus_tag="IV142_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP8486479.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(14467..14796)
                     /locus_tag="IV142_002923"
     CDS             complement(14467..14796)
                     /locus_tag="IV142_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP8486480.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(15054..15425)
                     /locus_tag="IV142_002924"
     CDS             complement(15054..15425)
                     /locus_tag="IV142_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8486481.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            15570..15755
                     /locus_tag="IV142_002925"
     CDS             15570..15755
                     /locus_tag="IV142_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8486482.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            16267..16485
                     /locus_tag="IV142_002926"
     CDS             16267..16485
                     /locus_tag="IV142_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486483.1"
                     /translation="MRQERPEGVHTGPACVKSHTARCIRKKEAAHFAEAWGVPNKQNE
                     NAAGGQVVDQNIRKGYSDPLLASIIGMF"
     gene            16698..17357
                     /locus_tag="IV142_002927"
     CDS             16698..17357
                     /locus_tag="IV142_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HAP8486484.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            17360..18703
                     /gene="vanS"
                     /locus_tag="IV142_002928"
     CDS             17360..18703
                     /gene="vanS"
                     /locus_tag="IV142_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP8486485.1"
                     /translation="MERKGIFIKVFSYTIIVLLLVVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQNKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLGNIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            18874..19680
                     /locus_tag="IV142_002929"
     CDS             18874..19680
                     /locus_tag="IV142_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HAP8486486.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            19698..20525
                     /gene="vanW"
                     /locus_tag="IV142_002930"
     CDS             19698..20525
                     /gene="vanW"
                     /locus_tag="IV142_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HAP8486487.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATIPEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            20522..21493
                     /locus_tag="IV142_002931"
     CDS             20522..21493
                     /locus_tag="IV142_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAP8486488.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            21486..22514
                     /gene="vanB"
                     /locus_tag="IV142_002932"
     CDS             21486..22514
                     /gene="vanB"
                     /locus_tag="IV142_002932"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAP8486489.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKFG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            22520..23128
                     /locus_tag="IV142_002933"
     CDS             22520..23128
                     /locus_tag="IV142_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAP8486490.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
BASE COUNT         5884 a         6039 c         6436 g         5130 t
ORIGIN      
        1 ggcccaaaag cataactgtc cccttgtccg gctggactac cagcaggaac agggccttgc
       61 aagcagcctg ccgctgggcg tcaatcagat caagatacag cgcagcctta cgacttccag
      121 cgtggccgtg ttcgtcccct ttgtgacgca ggagcttttt cagggcggcg cggccatgta
      181 ttacggcatc aacgccaagt cccgcaacat gattatgctg gacaggaagc aggcccgttg
      241 ccccaatgcc ttgaagctcg gcacccccgg cagcggcaaa tccatgagct gtaaatccga
      301 gatcgtcagt gtgttcttaa cgacgcctga cgatattttt atttcagacc cggaggccga
      361 atattacccc cttgtcaaac ggctccacgg acaggtaatc cggctgtcgc ctaccagcaa
      421 ggactttgtg aacccgctgg acatcaacct gaattattcg gaggacgata acccgctggc
      481 cctgaaatcc gactttgtgc tgtcgttctg tgagcttgtc atgggcggca agaacgggct
      541 ggaagccatt gaaaagaccg tgatagaccg ggctgtgcgc gtgatctacc ggccctatct
      601 ggcagacccc cgcccggaga atatgccgat cctgtccgac ctccacaaag cgttgttaga
      661 tcagcatgtc ccggaggctg accgggtggc gcaggccctt gacctgtatg tgtccggctc
      721 cctgaacgta tttaaccacc gtaccaacgt ggacatcggc aaccggcttg tgtcctttga
      781 cattaaggag ctgggcaagc agctcaaaaa gctggggatg ctcattgtcc aagaccagat
      841 atgggggcgc gtcacggcca accgaagcca aggaaaggcc acatggtatt ttgccgacga
      901 gttccatttg cttttgaaag aggaacagac ggcggcctat tctgcggaga tttggaagcg
      961 gtttaggaaa tggggcggca ttccaacagg tgccacccag aacgtcaaag acctgctttc
     1021 ctccccggaa attgaaaata tccttgaaaa cagcgacttc attacgctgc tcaatcaggc
     1081 gtccggggat agaaaaatcc ttgcggaacg cttgaatttg tccaccgaac agcagaagta
     1141 catcgacaat tccgaacccg gcgagggcct tttgatcttt gaaaacgtgg tgctgccctt
     1201 taccaacccc atcccgcaca acacccagct ttacaagatt atgacgaccc gcttaaacga
     1261 ggtggcggga gtatgacccg gcgacgccac aaaggaggtg aaccaccatg aaagagctga
     1321 aagccaaggc gaaagtgacg caggccatga cccgcgacgg gctgacgctg gaaaatcagg
     1381 ccaccggcga ggtggagaac atttccagcc gggaggccga acaggatttg tccgcgccgg
     1441 ggccgggcgg caccgccgat aagctgctgg aacgggccga cgccatccat gacggccata
     1501 aggccaagaa agccgcgaag caggccgggg ctgtggccgc cgagggggac gctgccctgc
     1561 accggccatc cgcccggctc caactgtcgg aggaagaacg ggccgacccg gatttacagc
     1621 cctatatccg aaaatcggac aaacgggccg ataagctgga taaggcccgc gccgccatcc
     1681 ccaagaagcg cgtcccggtc aaggaaaccg tctttgacga ggcaagcggc aaagcaaaga
     1741 ccgtcctgca ctttgacaaa caggacaagg ggcctccctc cctgaaaccc aatccggcag
     1801 gccgtcctgt ttccgaggtt tttctattcg gccacgggaa aatccatgaa gtcgagcatg
     1861 aaaacgtggg tgtggagggc ggccataagg gggaggaact tgccgagcgg caggccggaa
     1921 aagccatccg cagcaccatc cggcaccaca ggctgaaacc ctactgggac gcggaaaagg
     1981 ccgagcgcag gctattgtct gccaacgcgg agtatttcta ccagaaatct ttgcgggata
     2041 acccggagat cgcacaggcg gccagcaacc ccatttcccg gttctggcag aaacagcaga
     2101 tcaagcgcca gtacgccaag gccgcccgga cagcgggcca gaccgcaggc gcggcggcac
     2161 agggcgcggc caagacagcc ggggcctcca cgaaagcggc caaggccgca gccaaagaaa
     2221 gtgaacgggc ggcctccttt gtggcccgcc actggaaagg ggcgctggcc gtccttgtgt
     2281 tggggctttt gatgctgctt atcatcggcg gcttacagtc ctgcaccgcc atgttcggca
     2341 gcgccggaag cggcatcacg gcgtcctctt acttttccga ggatagcgat atgttggccg
     2401 ccgaggacgc ctacgcggag ctggaagccg agctgcaaga ctatctggac aactacgagg
     2461 ccacccatga ctatgacgaa taccattttg accttgacga gatcagccat gacccctatg
     2521 tgctgatttc cattctgtcg gcgctccatg acgggatatt taccatcggg gaggtacagg
     2581 gtgatttgtc catgctcttt gacaagcaat atatcctgac agaaaacgtc gtggtagaaa
     2641 cccgttaccg caccgaaacc gatacatgga cggacgcgga gggcaacacc catacagaca
     2701 cctaccaagt gccgtatgac tattacatct gctacgtcac gctggaaaac ttcgacctgt
     2761 cccatgtgcc agtctatatc atggacgagg aaacgctaag tttctattcc ctgtatatgt
     2821 ccacgctagg aaaccggcct gatctgtttt ccggcagcca gtaccccaac gcctccaccc
     2881 tgacgccgcc gacctactac gacgtacccc cggaggcttt ggaggatgcc acctttgccg
     2941 ccatgctcaa agaggcggaa aaatatatcg gttatcccta tgtgtggggc ggcagctctc
     3001 ccagcaccag ctttgattgc agcggcttca tttcgtgggt aatcaatcat agtggatgga
     3061 acgtgggacg gctgggggcg cagggcctat ataacatctg cacccctgtt tccagttcgc
     3121 aggccaagcc cggcgacctt gtatttttca agggaaccta cgacaccccc ggcgtgtccc
     3181 acgtcggcct gtatgtgggc aacaatgtga tgctgcattg cgggaacccg atttcctaca
     3241 cgaaccttac ttcaaactac tggcaatccc atttttacag ttacgggcgt ttaccttaac
     3301 gccagaaagg agctttcaat tatggcaacc aaacttgacc gcatcgaaaa ggacatccag
     3361 aaaaccaagt ctaagatcgc ggagtttcag aaacagcttc gggagctgga aacgcagaaa
     3421 accgaacagg agaatttgca gatcatccag cttgtgcgcg gcatgaacat gaagccggag
     3481 gaatttgccg cgttcttacg cagcggcgca atgcaggccg cccctgccgc aaccccttac
     3541 catgaacagg aggacaatgc ccatgaagaa taagaccaga cggatttccc gtattctccc
     3601 cgcacttctg gccgtgatgc tctgcgtcac ggcctttccc gttaccgccc ttgcaggcgg
     3661 caatgacccg gcaccggccc cgctgccgga ggctacaacc gaacccgcta cgggcggttt
     3721 ggagcctgaa acagacgaaa ccggcaccgc cacggacggg acagaaaccg aggccgaaaa
     3781 agataccggc cctctgaccg gcaaggacat caccgacctg ttttccgcgc tgttcggttc
     3841 caaggtgagc attgccgcca cggacgacgg catccagatc accaccggca ccgacaaaaa
     3901 agaacctgaa cagaccggca ccgtcaccac caacggcggc aggctgaatg tccgcaccgg
     3961 cgcggggctt gacaagaccg cgttcaccca gcttcccaat gggacgacgg ttgaggtagt
     4021 cggcacagac ggcgactgga ttaaaattct cttgccggag cgcatcggct atgtccattc
     4081 cgactatatg accgtcagcg aaaaggaagt agcggctacc ggcgagggcg gtttttccct
     4141 ctccattgac ccggaggaaa ttgcttctct gctggaactg ttcaatggcg gcggggccag
     4201 cgccgccctg tccccggacg ggaacctgtc actgattgac gacatcggca gcgccgcgaa
     4261 aaatggcaag cagtttatca ccgtggaaac caaaaacggc aatgtgtttt atctgattat
     4321 tgaccgggac gacgagggaa aagaaactgt ccatttctta aatcaggtgg atgaagccga
     4381 tctgctgacg ctgatggagg acggggaaac cgtcaccccc gctgctgtct gctcctgtac
     4441 cacgaaatgc aaggccgggg ctgtgaacac gaattgcccg gtatgtaaaa ccaacctgac
     4501 cgagtgcagc ggcccggagc cgcaggaacc ccagccggag gaacccgaag ccccggagga
     4561 agaacccaag ggcggcgcgg gcggcctgat cgtgttcctg ctggtggcgc tggcgggcgg
     4621 cggtgcggcc ctttactatt tcaagttcaa aaagcccaag gctgaaacca ccggcagcga
     4681 cgatttgggc gagtatgatt ttggggagga cgaggatttg gaggacgagg aacccgaaac
     4741 cgagtttgac ccggaagctg attttgtgag tgaacaggag gacgaggaat gacatataaa
     4801 cttgtaatcg cggaaaaacc cagcgtggcc cgcagcattg cgggcgtgat cggggccacg
     4861 gagaaacacg acggctattt ggaggggaac ggctatctgg tgagctggtg catcggacat
     4921 ctggtgtcct ttgccgacgc cggacgctat gacgagcgtt tcaaaaaatg gcgctatgag
     4981 gatttgccca ttctcccgga aacatggcag tacatcgtcc ccgacgacaa gaaacagcag
     5041 tttgacgttc tctgttcgct gatgcagcgc cccgacgtga ccggccttgt gtgcgccacc
     5101 gacgccgggc gcgaggggga attgattttc cgtttcgtct accagatggc gggatgccaa
     5161 aagccctttg agcgcctttg gatttcctct atggaggaca gtgctattaa ggacggcttt
     5221 gcccacctga aaccgggcgg cgactatgat ccgctgtatc aatcggccct gtgccgggcc
     5281 aaggccgact ggctgatcgg cattaacgcc acccggcttt tttccgtcct gtaccacaag
     5341 aacctgactg tgggccgcgt ccagacgccg accttaaaaa tgctggtgga cagggacgcc
     5401 aagattaccg atttcaagaa agagaaatat cacatcgtcc atatcacggc gggcggcgcg
     5461 gacgcggtaa gcagccgctt ttccgacgct gccgaggcaa acaccgtcaa ggcggcttgt
     5521 gcaaaagcgc aggccgtttg cgtttccgtg acgcgggaga aaaagacgga acagccgccc
     5581 aagctctatg accttaccac cttgcagcgg gaggctaacc ggcttttcgg ctataccgcc
     5641 aagcagaccc ttgactatgc ccaggcactg tatctaaaga agctgctgac ctatccgaga
     5701 acggatagtc aatatcttac cgacgatatg ctgcccgccg tggaaagcct tgtgtccggc
     5761 ctgtgggagc tggtgccgtt tgccaagggc ctgaaccttt ccccgcagtt tgaccgcatc
     5821 ctgaacagca aaaaggtgtc cgaccaccac gccattatcc ccaccgctga atttgtgaag
     5881 cagggctttg gcactctggc cgagagtgaa aaaaagctgc tgtccctgat ctgctgcaag
     5941 ctcttatgtg ccgtggccgg gccgcatatc tatgaggccg tcaccgccac atttacctgt
     6001 gccgggcagg agtttaccgc caagggcaag accgtccttt cccccggctg gaaagagatt
     6061 gaacgccggt tccgttcttc cttaaagacc gacgccgacg aggacgccga ggcggtgcgg
     6121 gagctgccgg agctttgcga gggccagacc tttgcagacg tggccgcctc tgtcaccgag
     6181 catttcacca cgccgcccaa gccctacacc gaagatacgc ttttgtccgc tatggaacgg
     6241 gccggggccg aggatatgcc ggaggatgcc gagcgcaagg gattaggcac accggccacc
     6301 cgcgccgcca ttctggaaaa gctggtgcag atgggctttg tccagagaaa aggcaagcag
     6361 ctcattccca ccaaggacgg catcaatctg gctgtggtgc tgccggaggc gcttacttct
     6421 ccccagctta ctgccgaatg ggaaaaccgg ctgaccgaga tcgccaaggg gcaggccgat
     6481 cctgacgaat ttatggacgg catcgagacg caggcgcggg agctggtgca gacctattcc
     6541 tgtatcagtg aggaaaagca gaaattgttt caggccgagc gtgttcctat cgggacgtgt
     6601 ccccgctgtg gtgaggctgt ctatgagggc aagaaaaact actactgcgg aaaccgggcc
     6661 tgccagttcg tcatgtggaa gaatgaccga ttttttgagg aacgcaagaa agcgttcacc
     6721 ccgaagatcg ccgctgcgct gctcaaaacc ggcaaagtca aggtaaaggg cctctattcc
     6781 gtaaagaccg gcaaaaccta cgacgggacg gtgcttttgg ctgataccgg gggcaagtac
     6841 gtcaactacc gcattgaaca caggagctaa aggacagtaa cacagcaagc atagggagtg
     6901 tggctacact cccggtaaaa tcccccggcc gcgtccctgc cgggccgggg atttttgctt
     6961 gttggggaag tccccaaccc cctatttttt gaaacagaac agagaaagga gtgaccgcgt
     7021 gaacgaagta ttttctatcc aagtccataa ccggcagttt ttggaacagg gcaaacaagg
     7081 cgtctggctg gatttgccca ccacaaccga aacgctgcaa gccgccctgc gggatgttgg
     7141 cattactgcc gacaatccgc aggatttttt tatcaacggg tattcctgcc cggaggacag
     7201 acaccttgcc attccctatg acatggtgct ggccgccgac gtggacgagc tgaatttcct
     7261 tgccgcccgg ctgggccagc ttgacgccgc cgagatcgca gagctgaacg ccgccctgca
     7321 aaatccccgt ggcggctttg agaatatcgg ccagatcatc gactacaccg aaaatgtgga
     7381 ttactatgtg catcttcccg acgttagggg ggatggacag ttgggcgact actacctgaa
     7441 ccgttccggc atggtggata tgccggagga atggaaagcc gggattgata cggcgcagtt
     7501 tggacgccac attgcccagc aggaacaagg cgtttttacc cagtacggct atctggtgag
     7561 aagcggcgac gaatggcagc gcgtccatga gggccagccg gtgccggagg aataccgggt
     7621 gatgcactac ccccagccgg aagctgaccg ggaggaaccg caggcccagc agccggaggc
     7681 atccgaaagc cccgacaaag cgccgccccc ggttatcccg atcatgctgg acggcatgaa
     7741 cagcaaagaa cgcatgaaag tgattactga ccgtctggaa accggcgtac aggagctttt
     7801 tgaaagtagc cgctatatgg cttacttatc cgctatgtcc aagatgcacg attacagtct
     7861 gaacaatacg ctgctgatcg ccatgcaggg cggttcgatt gtggcgggct ataacaggtg
     7921 gcaggatgaa tttgaccgcc atgtgaagcg gggcgagaaa ggcattaaga tactggcccc
     7981 ggccccgttc aaggttaagc agcagatgga gaaaatcgac ccggccaccc agcagcccgt
     8041 gatcggcgcg gacggcaagc ccgtgaccga agaacaggaa gtgacggttc cggcctttaa
     8101 ggtggtgaca gtctttgaca tcagccagac cgagggcaag gattttccca tgccctatat
     8161 cagtgaactt accggcgacg tgcagcagta tcaggatttc tttgccgcgc tggaacggac
     8221 atcaccctat cccataagca ttgagccgat ttcagacggg gccaagggcc gctgcttcta
     8281 cgcaaaacag cgcattgcca tcaacgaggg catgagcgaa ttgcagaccc taaagaccgc
     8341 catccatgag attgcccatg ccaagctcca cgccattgac cgggaggccc ccgccgagga
     8401 acaggcggcc cggccagacc gcagcacccg cgaggtacag gccgaaagcg tggcctatac
     8461 cgtctgccag tattacgggc tggacacgtc ggactattct tttggctatg tggccgattg
     8521 gagcagcggc agggagttat ccgagctgaa agcctccctt gaaaccatcc gggccacggc
     8581 cagcgaattg ataaccgaga tcgacggcca ttttgcccag cttcagcagg agcgcgaggc
     8641 ccggcaggaa caggaggccg tcacggttga cgttaccccg gaggcagcgg ccccggcgca
     8701 ggccgaggaa gaacaggccc cggcccttga tccggcagcc gagccgcttg tgactgtgct
     8761 atggagcgaa agcccccagc taaaggacgg ccagcaaatg ccgttgcatg aggccgacgc
     8821 gctgtttgcc cgtctggatg cagcatatcc ggcagaggca ggctatgaca aaaccgcgtt
     8881 ccgcattgac tgtatctttc agggcgagcc ggacagctac gaggggcggc aggattttgg
     8941 agatagggac ggttccctga tcgaacatat ccagagctac caccaatttt acgcgcagga
     9001 cgaaaactgg aaaaagcatg taatcgctac aaccggcata gagggctggg agcaggacaa
     9061 ggcccatcgg gatatgctgt taggcgagtt tgtcccctat atgaagctgc attgcagcct
     9121 tgcccaaatg gaacagacgg cgcagggccg cttacagtcc ggggaaaccc tgacgccgga
     9181 ggaaacggcc tatttggggg cgcttctctc ttatgtgcag gagtgccgcc ccatgctcaa
     9241 tcagggccag tatcagttgc cggagccgcc gaaactggct gacttcgacc agagcttgca
     9301 ggactacaaa gcacaggtac aggccgagat cgcgcaggag gccgccgcag ccggtatgac
     9361 cgtggaggaa tacgcggcgg ctggctatga agcaccccaa caggacacct tttccatcta
     9421 ccagcttcga ggcggggagg aaacccgcga ctatcggttt gagccatacg agcgtttaca
     9481 ggccgcaggg ctggccgtgg acagtgccaa ctatgacctt gtttatacgg caccgctgga
     9541 taaaagcacg tctttggagg atatttaccg cacattcaac atcgaccacc cggcagattt
     9601 taggggacac agcctttccg tttccgacgt ggtggtgctg caccagaatg gacaggacac
     9661 cgcccactat gtagacagct tcggctatat ggaagtgccg gagtttttgc gggaaaatcc
     9721 cctgcgtacc gccgaactgt ctacggagca gaacgaaaac atgattgacg gcattatcaa
     9781 caatacgccg cccgaaccgc agccggagga acccgaacag ctatcttttg aggatgtggc
     9841 agttgcttcc ggccagacac aggaggaacc cccggaggct gcgcccccgc aaacggctgt
     9901 ccgctattat cccatcaatg agggagccgc ccgccgcgcc aaggaagcca tgagcttttc
     9961 tgactaccgg cccggcagcg ccacggcgga ataccggcac tatgtcgatc aggccgtgga
    10021 aattgcggag cgccagaaaa agcgtgtgga gccagagtat cacgaaaaga tagacagcct
    10081 ccttgacacc tacgcccgca agctggccga gaacatgaat aagggctatg agattaccgc
    10141 ccgcgtcccc tctgtcctga ttgccgggcc gtccaatttc ccggtacggg cgaaagaaaa
    10201 gcagaacgcc gccagtgacc gcaacatgga ggaatttcag tacattcagg gcttgcttga
    10261 caaaatccgc agcaccggca tgggtggtat cagcgccgac gatccgcagg ccgtttccaa
    10321 gctggaaaag aaactggaaa agctggaagc gtcgcaggag ttgatgaagg ccgtcaatgc
    10381 ctactaccgc aagcatggga cgctggacgg atgcccccat ctgaccgaaa gaggcattga
    10441 aaacctgaaa gcggatatgg cgtcgggctg gcactatgag aaaaaaccgt tccagtcctg
    10501 gcagctctcc aacaataacg ccgagatacg ccggttaaag ggccgcattg aggaattgac
    10561 ccgccaaaag gaggcggcct atgtgggatg ggagtttgac ggcggcactg tggagatcaa
    10621 ccgggtggcc aaccgcctgc aaatcttctt tgaggggaag ccggatgctg ctgtacgcga
    10681 cgagctgaaa agcaacggtt tccgctggtc gcccaaggcc ggggcatggc agcggcagtt
    10741 aaacgacacc acaatccgcg tcgctgaccg tatcaagtgc atccagcccc tttccggcga
    10801 aaagcccagc gccttacagc ttgcggcccg cagggagaaa gaaaagccct ctatccgggc
    10861 gcagctcaac gcggccaaga ccgaacaggc caagcagcca ccggcgcggg aaaaatcaaa
    10921 gggattggag gtagagtaaa tgcagaagtt tgaaaatgtg gacgttctgg ccgcgctgga
    10981 acagatcatg cggcagaaca ccgcgtttta tcaaagtgac tttggcattg acaaagacat
    11041 tatcaggcgg gatgccgcca gcgatcaggc cgcagacaag accctgctct ggatgtcccg
    11101 gccctccggg acgtactgtt tccgggaacg tgatgttttc ctgaaagaca cccgacagta
    11161 caacacatgg aaattttacg gggagcagac ccgtgacaag atactggcct acgcggtgga
    11221 gctgaccggc acacagggcg gcactatccg gggcaatctc tatgagctgg actatccgca
    11281 gcatttccgg cacgtcattg aaaccgccca gccggtgagt gtgaaccggc tcttttatga
    11341 gcatggcacc cgtgacatcc cggagagcca gcgttttgac ggaagccctg accgggtatt
    11401 gggaaacttt ttgtactatg aggcccagcc ccatgacccg gctgtgctgc aggaggcgct
    11461 acggacggag cagcacagcc gggggcgggc cgtccccggc gatttcaaag tccacgtcac
    11521 cgctttgcat gacagccgga ttgaaaccga ggcccggcgt attgtggaca gcctgaaaga
    11581 tctgtccgcg cctaacagcc ccaataaaac gcatttcatg gtggagatct ccccgcactt
    11641 tgcggcgatt gcttccacca aggataatga ccggctcttt gccatgctgc cctataaatc
    11701 cctgtgtttt accgggatga aaggcaggca tggcgtgtat gccgtgatta accgggacga
    11761 gcgccgggat gtgagtattc gcaggccccg gccctctatc cgcaagcagc tatccgacag
    11821 caaacaggcc gccagcccaa aaaaggcggc ggccagaacc aaaaagaatg aattggaggt
    11881 ataaggaatg atgcacttta ccgtggagga agaaaacctg atctgtatgt accacaatgc
    11941 agaccggcgc agaacgataa acaggctcac cgccgtcatg ccggttatgg atggggatat
    12001 gcgggcgcag gcccagcaga ccctttccaa gctggaacgc atgacggatg ccgactatga
    12061 cggccagaag ttccacttca cagacgaata acgaacagca gacgccgggc gcggcggcca
    12121 tgtggtcgct tgcgtccggc gtcttttctt tttgcctatt cctacgcatt tagaacccgg
    12181 tattgtcggg gataggatga agtattcgct tgcccctgca aacatgcggg agaagccttg
    12241 cagcgggcgg ttttcaatcc cttaaatgcg taggttgcag ctataattag acccctgaaa
    12301 cagatatagc cgcgcagcgg gtgtatctgt ttcaggggtg tgcaggtgat agccgcaaag
    12361 cggcgtaaga gggtgcagcc ctcttgccgt ctatctctgt tccagcggga agctcggcaa
    12421 tgcttccagc agtttcaggg aaatgcgctg ccgtatatct tcgtcaatga ctttcttctt
    12481 tttgccgttt gcctgtctga tctccaccgt ggaaagctcg ttgatgtagt cagcataatg
    12541 ggccaacact ttttccgtgg cccacctctc accggcaacg gcggctttga tcgtttcata
    12601 gtccagcagc ttttcttgtc cgtccatcct gatacccccg tcagactgat tttttctatg
    12661 ataccatatt cggaagcatg gaaccagaga aaacggcgct tttctgctgg taaattctac
    12721 gcatttagaa cagcacttcg gcggggatag gataaaacac ccacataccc cgctgaccat
    12781 gccggaaaag ccttgaaata taagggcttt tacagctcta agtgcgtagg tttccagtat
    12841 cttttccgct ggcgctcggc ctctttgcgg cggcgcatcc gtttggagca ggccgcgcag
    12901 tatttggccc ggttagactt cggcgtgaac gtggccccgc actcacagca gcgtttcact
    12961 tcgtcccggt ctttggtgat ctcggcatac agggccgcat ccagcgggag gacagcggcc
    13021 ttaaaccagc ggcacagaag cgaataggaa atgctctgta cgcaaataca ttcttcgccg
    13081 tcgtccagca acaggcagtt gccgtcacaa tagttgcagc actcatgcgt cagcttccgg
    13141 gccttgcgat attgagggta attcattcgt ggaatactca tagctcttgc gtccattcct
    13201 ttcccggcgt catacgcaaa atgccgtcca cattctgctt gatgatgccg tattcctgca
    13261 tctgcttttt cgcttcgccg tacttctcat acagccgttc cttttcctcg gccaatcgct
    13321 tgtactcggc tttcagggcg tacagatcgg ggagcttttt cacaccctgt ccctttaggg
    13381 cctttgccgc agcttcatac aaaatcaacg tgctttcatg ctcacgccgg aattttgcct
    13441 tatccgctgc attgcggtat tccatagcca cgggccagag ctgcttgtag gtagaaatat
    13501 tctttatcag caccgccata tccgcaagcc ggtgttccat agctttgagg gtagcggcgg
    13561 cctcgtcgct ggcggcggtg atctcggcca ctttgctttc caaatccggg taggtgtcta
    13621 tcccatgttc agtcaggaaa ttcaaagtct ttgcggcctg cttcaaattg tggagcttcg
    13681 cccacctctc atagcctgcc gactgctggg ccttaatgtt gttttccaga tcaatccgca
    13741 aagaaatccc cttgcgctct ttgggcgctc tggtgataac gcggcctttg atacgctctt
    13801 tgatggcttc ctcggtgtag gcttccccca atgtcttgca gcgggtaaag cgttcctgtc
    13861 cgggagcgcg gaaagaaata aactttcccg gcttgatctc atagccctgc gcctgcatga
    13921 gccgcaggaa atcatcaaaa tcttttgcct gcgggatagc ggcgtcaatg gctgctttga
    13981 gcttcgcttt ccagctcgtc cctttccgct gggcgtccca ctcggcatag ctcttgccct
    14041 tactctggcc gggcttcacc acggacaatc cgttttcctt gcacagcctg tcgctggtgc
    14101 gccggatata ggcatagctc tgtttgttgg aaatgtactt gcgctgattt accatatcca
    14161 cggcacagaa aataatgtga ttgtggacgt ggcccttatc aatatgggta gtcagcacat
    14221 agggatattt cccttgcagc acttcctcgg caagctgctt cccgatctca tgggcctgtt
    14281 ccggcgtggc ttcgccgggg gcaaaggatt gtatcaggtg gtggcccaga ttattgccct
    14341 tttggtatgc ctgcgccagc agcatttcaa attcaatgtc cgctgtttca taagagcagg
    14401 caaaggaggc caccagcagc ttgccgtcag ttttgtccgg gttctcgatg tagtcaaggg
    14461 cttttttcag cgttgacttg ataggatgta tctttgtaac tgccatatct gcgccagcgc
    14521 cccctttata tcctcgatgt cctgtgcgta caccggcccc ataccgttca gcctctttgc
    14581 aatctgattg acgttgacgc cgatcttctg tatctcggcg gtctgcgccc ggatgtcgct
    14641 ggtgtccatg ttgacgatat agccgtcaat gagcatcttc cgggcgtagg ctgaaaagtt
    14701 cttcgtatgc agcagcgcca ttttctgttc aatcatagcc cgttcctccg gcgtcacagg
    14761 gacgcgcagc acgatttttc gctttctgtt tgccatgctt caccgtcctt tccattcagg
    14821 ggtttgggga tttccccaac aagcaaaacg gacgcggccc ggcagggacg gggcaggccg
    14881 tttttccggg agtggctaca ctccctatgc ttgctacgtt actgtttctc cactaacaag
    14941 acgtttccaa agccccaaac tacccgcaaa atagccctct ggaaagaaaa aagtgcaaaa
    15001 aaatagaagc tacgctgtgc agctcccgct aaaagtaaaa cagaggatgc cagttacaca
    15061 tcctccgttt cattcttctt cggcttttca attagtatgg cgctgtcaat cgcgccctcg
    15121 atgatcggca ggtattcttc ggggcagagt ttcagcttgt ggctgaccct ttgccgcagt
    15181 tcgctttcct cccgcatgat ctccgggtta aagtagcgtt ccacaggaag cccgcaggct
    15241 ttaataagct gtatgacaac cggcagactg ggaatggccc cgtcgttttc gatgttggca
    15301 agatagcgcc agtcaatatt tattttctct gccattgctt tgcgcgaaac gcgctttgct
    15361 tgccgggccg ctttcacatc cgcgccgaaa gtttcaaagc cgggacaatc ttcaatattc
    15421 gccatatcgc acctcccact tacattgtat atttcatact ctgcccgtgg aatgttggta
    15481 tatgcacctt tcttaaagtt tatatttcac attttgtttc ttttattctt cgagcaaaag
    15541 aattataatg taccctgtgg aggtgtacta tggactacat gacgttgaaa gaggcaggcg
    15601 agaaatgggg tgtgccccca cgatggataa actactattg tgctgcggga cgcatccccg
    15661 gcgctgtaaa gatggcgacg atctggctca ttcccaaaac tgcggaaaag ccgattgaca
    15721 ggcgtacaaa gaaaggaaag gcggttactg aatagtataa tgaaaccggc gcattttgga
    15781 tatggttttg aagagagaga aagcatggta ttttgtcacg gaaaaactat aagatgatag
    15841 atggcaggct gctccagacc gataaaaagt attctcatct gaaacagaaa gaaaagattt
    15901 acacatggat gtatgaagaa gcgaaacttt atcatgacag aaatggaaaa tgcccgaaac
    15961 aaagacgagg aagctgctgc tgtatcagcc gtatatggta gaattgaaaa ggccggcatc
    16021 tggattcttt atggtgaagt ctccagacat tggataactg ctggtctaat gcgcctttga
    16081 gtatggggtg attgatttta ataccgggga ggtacggctt tttgaggaaa atattctggg
    16141 atttacattt ggcgattatt acatttcctg taactgcaag gacacggatg atgaagatat
    16201 gaggaatata gacttgctga ttttctactg tcttgaacgg tattaggact gaatctgctg
    16261 cgcggagtgc ggcaagagcg accggaggga gtacacacag gccctgcctg tgtgaaaagc
    16321 cataccgcaa ggtgcattcg caaaaaagag gccgcgcatt ttgctgaggc ttggggtgtc
    16381 cccaacaagc aaaacgagaa cgccgcaggc gggcaagtgg tggatcagaa tatccgtaaa
    16441 ggatattctg atccattgct tgccagtatt atcgggatgt tttgacaacc gctgtaatcg
    16501 aaacatccca aacattaaga aggtgttaat tattattatc ttgatttaat tcgtttaaac
    16561 caataatata gatacataca ccatgaaaag aagcgagtat cccaatgacg gatgattaaa
    16621 atccgaaaag aaatcgcttt gaaactacag ggaaactaca gactgttatg ttatcttctt
    16681 aaaatggagg gatttttatg tcgatacgaa ttctacttgt cgaggatgat gatcatatct
    16741 gtaatacagt aagggcgttt ctggctgagg caggatatca ggtggatgcc tgcacagatg
    16801 gaaatgaggc atacaccaag ttttacgaaa acacttatca actggttatt cttgatatta
    16861 tgctgcccgg tatgaacggg catgaacttt tgcgtgaatt tcgtgcgaaa aatgatactc
    16921 ccattctgat gatgacagcc ctgtcggatg acgaaaacca aatccgggcg tttgatgcag
    16981 aggcagacga ctatgtaaca aagccattca agatgcagat tttactaaag cgggtggaag
    17041 ccctgctgcg gcgcagcggt gcgctggcaa aggaaatccg tgtcggcagg ctgacacttc
    17101 tgccggagga tttcacggta ctttgtgacg gtacagagct gcccctgaca cgaaaagaat
    17161 ttgaaatcct tttgctgctg gtgcagaaca aaggcagaac cttaacgcat gaaatcattt
    17221 tgtcacgtat atggggatat gactttgagg gtgatggcag cacagtccac actcatatca
    17281 aaaatttgcg ggccaagctg ccggaaaata tcatcaaaac cattcgcggt gtaggatacc
    17341 gattggagga atcataataa tggaaagaaa agggattttc attaaggttt tttcctatac
    17401 gatcattgtc ctgctactgg ttgtcggtgt aacagcaaca ctgtttgcac agcaatttgt
    17461 gtcttatttc agagtgatgg aattacagca aacagtaaaa tcctatcagc cattggtgga
    17521 actgattcag aatagcgata ggcttgatat tcaagaggtg gcagggctgt ttcactacaa
    17581 taaccaatcc tttgagtttt atattgaaga taaagaggga agcgtactct atgccacacc
    17641 aaatgccaat acatcaaata gttttagacc cgactttctt tatgtggtac atagagatga
    17701 taatatttcg attgttgctc aaaacaaggc aggtgtggga ttgctttatc aagggctgac
    17761 aattcgggga attgttatga ttgcgataat ggttgtattc agccttttat gcgcgtatat
    17821 ctttgcgcgg caaatgacaa cgccgatcaa agccttagcg gacagtgcga ataaaatggc
    17881 aaacctgaaa gatgtaccgc cgccgctgga gcgaaaggat gagcttggcg cactggctca
    17941 tgatatgcat tccatgtatg tcaggctgaa agaaaccatc gcaaggctgg aggatgaaat
    18001 cgcaagggaa catgagttgg aggaaacaca gcgatatttc tttgcggcag cttctcatga
    18061 gctaaaaacg cccatcgcgg ctacaagcgt tctgttggag ggaatgcttg gaaatatcgg
    18121 tgactacaaa gatcattcta agtatctgcg cgaatgcatc aaaatgatgg ataggcaggg
    18181 caaaatcatt tccgaaatac tggagcttgt cagcctgaat gatgggagaa tcgtacccat
    18241 agctgaaccg ttggacatag ggcgcacggt tgccgagttg ctgcccgatt ttcaaacctt
    18301 ggcagaggca aacaaccagc ggttcgtcac agatattcca gccgggcaaa ttgtcctgtc
    18361 cgatccgagg ctgctccaaa aggcactatc caatgtcata ttgaatgcag ttcagaacac
    18421 gccgcaggga ggcgaggtac ggatatggag tgagcctggt gctgaaaaat gccgcctttt
    18481 tgttttgaac atgggcgttc acattgatga tactgcgctt ccaaggctgt tcaccccatt
    18541 ctatcgcatt gatcaggcgc gaagcagaaa aagtgggcga agcggtttag gacttgccat
    18601 cgtacaaaaa acgctggatg ccatgagcct ccagtatgcg ctggaaaaca cctcggatgg
    18661 cgttttgttc tggctggatt taccgctcac atcaacattg taaatattta aaacttaaat
    18721 gattttgacc gacaggtata accctgccgg tctttttttg ctgctacagg aaaactacag
    18781 actggctaca gggaaagtac agatgcgctt gccataataa caatcgtacc agccacaaat
    18841 cgtagtttta ttgcaaagga ggcattcgat caaatggaaa aaagcaacta tcattccaat
    18901 gcggatcatc acaaacggca tatgaaacaa tccgtggaaa agcgggcttt tctatgtgcg
    18961 ttcattattt cgttcacagt ctgcacgctg tttttggggt ggagactggc ttccgtattg
    19021 gaggcaacac agataccgcc catccctgca actcatacag gcagcagcac tgacgtagtg
    19081 gagaatttgg aggaaaacgc tcttgccacc gccaaagaac agggagatga acaggaatgg
    19141 agcctgattt tagtgaacag gcagaacccc atccccgcac agtacgatgt ggagcttgag
    19201 caactatcaa atggtgagcg gatagatatt cggatttctc cctatcttca agatttgttt
    19261 gatgccgcaa gaactgatgg agtttacccg attgtcgcat ccggataccg aacaacagaa
    19321 aaacagcaag aaattatgga tgaaaaaatt gccgaatata aggcgaaagg ctacacctct
    19381 gcacaggcta aagcggaagc agaaacttgg gtggccgtgc cgggaacgag cgagcatcag
    19441 cttggtcttg ctgtggatat caatgcggac ggaattcatt caacaggcaa cgaggtttat
    19501 agatggctgg atgaaaacag ctatcgtttt ggttttattc gtcgctaccc accagacaag
    19561 acagagataa ccggtgtgag caacgagccg tggcattacc gatatgttgg catcgaagct
    19621 gccacagaga tgtacaacca aggggtttgc cttgaggaat atttaaaacc agaaaaatga
    19681 gaaaaggatg ttatgttatg gacagaaaaa gattgacaca gcgtttcccg ttcctgcttc
    19741 caatgagacg ggcgcagaga aaaatgtgct tttatgcggg aatgagattt gacggctgtc
    19801 gctatgcgca gacgataggg gaaaaatcgc tttcccattt gctctttgaa acggattgcg
    19861 cattatacaa ccacaatacc ggatttgaca tgatatacca ggaaaacaag gtgttcaact
    19921 taaagctggc agcaaagacc ttaaacggct tattgataag accgggggaa accttttctt
    19981 tctggtggct ggtacgccat gcggacaaag atacccccta taaagacggc cttacggtga
    20041 ccaatggtaa actcaccacc atgtcgggcg gcggtatgtg ccagatgagc aatttactat
    20101 tttggatgtt cctgcatacg ccattgacaa ttatccagcg cagaggtcac gaagtaaagg
    20161 agtttccaga gccaaacagt gacgagatta aaggggtgga tgcaaccatt ccagagggct
    20221 ggattgattt aaaagtgcga aacgataccg actgcaccta ccaaatatgg gtgaccttag
    20281 atgatgagaa aatcatcggt caggtgtccg ccgacaaaga gccccaagca ttatacaaaa
    20341 ttacaaatgg cagtatccag tatgtccgtg aaagtggcgg gatttatgaa tatgcccagg
    20401 ttaaacggat gcaagttgcc ttaggtaccg gggaaataat agattgcaag ctgctttata
    20461 caaacaaatg caaaatctgt tatcccctcc cggaaagtgt ggatattcag gaggagaacc
    20521 aatgagaaaa agtgtgggca ttactgtttt tggatgcgaa caggatgagg caaatgcttt
    20581 ccgcgcttta tcgccggatt ttcatattat ccctacgctg attagcgatg cgatatcggc
    20641 agacaacgca aaattggccg ctggcaatca atgcgttagc gtaggccata agtccgaggt
    20701 ttccgaggcg acaattcttg cgctgagaaa ggtcggggta aaatacattt ctacccgcag
    20761 catcggctgc gatcacattg atacgactgc cgccgagaga atgggaatct cggttggcac
    20821 ggttgcgtat tcgccggaca gcgttgcgga ttatgctttg atgctgatgc tgatggccat
    20881 acggggtgca aaacccacca tgcacgccgt ggcgcaacaa gatttcagat tggatcgtat
    20941 ccgggggaaa gaactggggg atatgactgt gggagttatt ggaaccggcc atatcgggca
    21001 agcggtcgtc aaaaggctgc ggggatttgg atgccatgtg ctggcctatg ataacagccg
    21061 aaaaatggat gcagattatg tccagcttga tgagcttcta aaaaacagcg atattgttac
    21121 gctccatgtg ccgctttgtg cggatacccg ccatctgatc ggtcagaagc aaattggaga
    21181 gatgaagcaa ggcgcatttt taatcaacac cgcgcgcgga gcgcttgtcg ataccggggc
    21241 gctggtggag gcgttggaaa gcggaaagct gggtggtgcg gcactggatg tgttggaggg
    21301 cgaggatcag tttgtttata ccgattgctc gcagaaagtg cttgaccatc cctttttgtc
    21361 gcagctttta aggatgccga atgtgatcat cacaccccat acggcgtact acactgagcg
    21421 tgtgctgcag gatactacag aaaaaacaat caggaattgt ctcaattttg aaaggagttt
    21481 acagcatgaa tagaataaaa gtcgcaatca tcttcggcgg ttgctcggag gaacatgatg
    21541 tgtcggtaaa atccgcaata gaaattgctg cgaacattga tacggaaaaa ttcgatccgc
    21601 actacatcgg aattacaaaa aacggtgtat ggaagctatg caagaagcca tgtacggaat
    21661 gggaagccga cagtctcccc gccatactct ccccggatag gaaaacgcat gggctgcttg
    21721 tcatgaaaga aagcgaatac gaaacacggc gtattgatgt ggctttcccg gttttgcatg
    21781 gcaaattcgg ggaggatggt gcgatacagg ggctgtttgt attgtctggt atcccctatg
    21841 tgggctgtga tattcaaagc tccgcagctt gcatggacaa atcactggcc tacattctta
    21901 caaaaaatgc gggcatcgcc gttcccgaat ttcaaattat tgataaaggt gacaagccgg
    21961 aggcgggtgc gcttacctac cctgtctttg tgaagccggc acggtcaggt tcgtcctttg
    22021 gcgtaaccaa agtaaacggt acggaagaac ttaacgctgc gatagaagcg gcaggacaat
    22081 atgatggaaa aatcttaatt gagcaagcga tttcgggctg tgaggtcggg tgtgcggtca
    22141 tggggaacga ggatgatttg attgtcggcg aagtggatca aatccggctg agccacggta
    22201 tcttccgcat ccatcaggaa aacgagccgg aaaaaggctc agaaaatgcg atgattacag
    22261 ttcccgcaga cattccggtc gaggaacgaa atcgggtgca ggaaacggca aagaaagtat
    22321 atcgggtgct tggatgcaga gggcttgccc gtgttgatct ttttttgcag gaggatggcg
    22381 gcatcgttct aaatgaggtc aataccatgc ccggttttac atcgtacagc cgctacccac
    22441 gtatggtggc cgccgcagga atcacgcttc ctgcactgat tgacagcctg attacattgg
    22501 cgttaaagag gtgataagta tggaaaatgg ttttttgttt ttagatgaaa tgttgcatgg
    22561 cgtccgttgg gatgccaagt acgccacatg ggataacttc acaggaaaac cggtggatgg
    22621 gtatgaggtg aatcgcatca tcggcacaaa ggccgtggcg tttgctctgc gcgaggcaca
    22681 aatccatgcg gctgcgcttg gctatggctt gcttttatgg gatggatatc ggccaagaac
    22741 tgcggtggac tgcttcctgc gttgggcagc gcaaccggag gacaatctca caaaagaaaa
    22801 attttacccc aatatagagc gagccgagtt gattacaaag ggttatgtgg cctcacaatc
    22861 cagccatagc cgtggaagcg caattgatct tacgctctac cacctggata caggggaact
    22921 tgtttcaatg ggaagtaact tcgattttat ggacgaacgg tcgcatcata cagcaaaagg
    22981 gataggggat gcagaggcac aaaatcgaag atgcttgcgt aaaatcatgg aaagcagcgg
    23041 atttcagtct tatcgctttg aatggtggca ctataagttg attgatgagc cataccccga
    23101 tacctatttt aattttgccg tttcataatg aaagtattgg ttttgtatca gattttggat
    23161 ttgataaaat ctatgctggt catggacaat cttttactgg aaagtggtta aagcatcttt
    23221 tgtaatacat tgcaaattat agtattcaat ctgcccagcg gcagaaaaga aaaaaaccgc
    23281 tgttgggcag acctttccca cgcccatttt gtattttgct ggcggcggtt ttgagaaatc
    23341 aaggccgccg ttcttttctg gcagcaaaag gtgtggcggc ccataggagg atgccgctat
    23401 gctgttttgc tattgccatg ctctaatcca cttccaccac tcaaaaaagc ggctctttgt
    23461 caataaggac tgatgagttg tgcaaaatc
//
LOCUS       DACDEG010000272        23488 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.guided.034, whole genome shotgun sequence.
ACCESSION   DACDEG010000272 DACDEG010000000
VERSION     DACDEG010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.guided.034"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1276
                     /locus_tag="IV142_002934"
     CDS             <1..1276
                     /locus_tag="IV142_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP8486491.1"
                     /translation="AQKHNCPLVRLDYQQEQGLASSLPLGVNQIKIQRSLTTSSVAVF
                     VPFVTQELFQGGAAMYYGINAKSRNMIMLDRKQARCPNALKLGTPGSGKSMSCKSEIV
                     SVFLTTPDDIFISDPEAEYYPLVKRLHGQVIRLSPTSKDFVNPLDINLNYSEDDNPLA
                     LKSDFVLSFCELVMGGKNGLEAIEKTVIDRAVRVIYRPYLADPRPENMPILSDLHKAL
                     LDQHVPEADRVAQALDLYVSGSLNVFNHRTNVDIGNRLVSFDIKELGKQLKKLGMLIV
                     QDQIWGRVTANRSQGKATWYFADEFHLLLKEEQTAAYSAEIWKRFRKWGGIPTGATQN
                     VKDLLSSPEIENILENSDFITLLNQASGDRKILAERLNLSTEQQKYIDNSEPGEGLLI
                     FENVVLPFTNPIPHNTQLYKIMTTRLNEVAGV"
     gene            1308..3299
                     /locus_tag="IV142_002935"
     CDS             1308..3299
                     /locus_tag="IV142_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAP8486492.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            3322..3573
                     /locus_tag="IV142_002936"
     CDS             3322..3573
                     /locus_tag="IV142_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HAP8486493.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            3563..4792
                     /locus_tag="IV142_002937"
     CDS             3563..4792
                     /locus_tag="IV142_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HAP8486494.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            4789..6870
                     /gene="topB"
                     /locus_tag="IV142_002938"
     CDS             4789..6870
                     /gene="topB"
                     /locus_tag="IV142_002938"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8486495.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            7019..10939
                     /locus_tag="IV142_002939"
     CDS             7019..10939
                     /locus_tag="IV142_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HAP8486496.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            10940..11884
                     /locus_tag="IV142_002940"
     CDS             10940..11884
                     /locus_tag="IV142_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486497.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            11888..12091
                     /locus_tag="IV142_002941"
     CDS             11888..12091
                     /locus_tag="IV142_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486498.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(12391..12627)
                     /locus_tag="IV142_002942"
     CDS             complement(12391..12627)
                     /locus_tag="IV142_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8486499.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(12810..13181)
                     /locus_tag="IV142_002943"
     CDS             complement(12810..13181)
                     /locus_tag="IV142_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486500.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(13178..14506)
                     /locus_tag="IV142_002944"
     CDS             complement(13178..14506)
                     /locus_tag="IV142_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP8486501.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(14467..14796)
                     /locus_tag="IV142_002945"
     CDS             complement(14467..14796)
                     /locus_tag="IV142_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP8486502.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(15054..15425)
                     /locus_tag="IV142_002946"
     CDS             complement(15054..15425)
                     /locus_tag="IV142_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8486503.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            15570..15755
                     /locus_tag="IV142_002947"
     CDS             15570..15755
                     /locus_tag="IV142_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8486504.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            16267..16485
                     /locus_tag="IV142_002948"
     CDS             16267..16485
                     /locus_tag="IV142_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486505.1"
                     /translation="MRQERPEGVHTGPACVKSHTARCIRKKEAAHFAEAWGVPNKQNE
                     NAAGGQVVDQNIRKGYSDPLLASIIGMF"
     gene            16698..17357
                     /locus_tag="IV142_002949"
     CDS             16698..17357
                     /locus_tag="IV142_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HAP8486506.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            17360..18703
                     /gene="vanS"
                     /locus_tag="IV142_002950"
     CDS             17360..18703
                     /gene="vanS"
                     /locus_tag="IV142_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP8486507.1"
                     /translation="MERKGIFIKVFSYTIIVLLLVVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQNKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLGNIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            18874..19680
                     /locus_tag="IV142_002951"
     CDS             18874..19680
                     /locus_tag="IV142_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HAP8486508.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            19698..20524
                     /gene="vanW"
                     /locus_tag="IV142_002952"
                     /pseudo
     CDS             19698..20524
                     /gene="vanW"
                     /locus_tag="IV142_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
     gene            20521..21492
                     /locus_tag="IV142_002953"
     CDS             20521..21492
                     /locus_tag="IV142_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAP8486509.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            21485..22513
                     /gene="vanB"
                     /locus_tag="IV142_002954"
     CDS             21485..22513
                     /gene="vanB"
                     /locus_tag="IV142_002954"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAP8486510.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKFG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            22519..23127
                     /locus_tag="IV142_002955"
     CDS             22519..23127
                     /locus_tag="IV142_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAP8486511.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
BASE COUNT         5883 a         6039 c         6436 g         5130 t
ORIGIN      
        1 ggcccaaaag cataactgtc cccttgtccg gctggactac cagcaggaac agggccttgc
       61 aagcagcctg ccgctgggcg tcaatcagat caagatacag cgcagcctta cgacttccag
      121 cgtggccgtg ttcgtcccct ttgtgacgca ggagcttttt cagggcggcg cggccatgta
      181 ttacggcatc aacgccaagt cccgcaacat gattatgctg gacaggaagc aggcccgttg
      241 ccccaatgcc ttgaagctcg gcacccccgg cagcggcaaa tccatgagct gtaaatccga
      301 gatcgtcagt gtgttcttaa cgacgcctga cgatattttt atttcagacc cggaggccga
      361 atattacccc cttgtcaaac ggctccacgg acaggtaatc cggctgtcgc ctaccagcaa
      421 ggactttgtg aacccgctgg acatcaacct gaattattcg gaggacgata acccgctggc
      481 cctgaaatcc gactttgtgc tgtcgttctg tgagcttgtc atgggcggca agaacgggct
      541 ggaagccatt gaaaagaccg tgatagaccg ggctgtgcgc gtgatctacc ggccctatct
      601 ggcagacccc cgcccggaga atatgccgat cctgtccgac ctccacaaag cgttgttaga
      661 tcagcatgtc ccggaggctg accgggtggc gcaggccctt gacctgtatg tgtccggctc
      721 cctgaacgta tttaaccacc gtaccaacgt ggacatcggc aaccggcttg tgtcctttga
      781 cattaaggag ctgggcaagc agctcaaaaa gctggggatg ctcattgtcc aagaccagat
      841 atgggggcgc gtcacggcca accgaagcca aggaaaggcc acatggtatt ttgccgacga
      901 gttccatttg cttttgaaag aggaacagac ggcggcctat tctgcggaga tttggaagcg
      961 gtttaggaaa tggggcggca ttccaacagg tgccacccag aacgtcaaag acctgctttc
     1021 ctccccggaa attgaaaata tccttgaaaa cagcgacttc attacgctgc tcaatcaggc
     1081 gtccggggat agaaaaatcc ttgcggaacg cttgaatttg tccaccgaac agcagaagta
     1141 catcgacaat tccgaacccg gcgagggcct tttgatcttt gaaaacgtgg tgctgccctt
     1201 taccaacccc atcccgcaca acacccagct ttacaagatt atgacgaccc gcttaaacga
     1261 ggtggcggga gtatgacccg gcgacgccac aaaggaggtg aaccaccatg aaagagctga
     1321 aagccaaggc gaaagtgacg caggccatga cccgcgacgg gctgacgctg gaaaatcagg
     1381 ccaccggcga ggtggagaac atttccagcc gggaggccga acaggatttg tccgcgccgg
     1441 ggccgggcgg caccgccgat aagctgctgg aacgggccga cgccatccat gacggccata
     1501 aggccaagaa agccgcgaag caggccgggg ctgtggccgc cgagggggac gctgccctgc
     1561 accggccatc cgcccggctc caactgtcgg aggaagaacg ggccgacccg gatttacagc
     1621 cctatatccg aaaatcggac aaacgggccg ataagctgga taaggcccgc gccgccatcc
     1681 ccaagaagcg cgtcccggtc aaggaaaccg tctttgacga ggcaagcggc aaagcaaaga
     1741 ccgtcctgca ctttgacaaa caggacaagg ggcctccctc cctgaaaccc aatccggcag
     1801 gccgtcctgt ttccgaggtt tttctattcg gccacgggaa aatccatgaa gtcgagcatg
     1861 aaaacgtggg tgtggagggc ggccataagg gggaggaact tgccgagcgg caggccggaa
     1921 aagccatccg cagcaccatc cggcaccaca ggctgaaacc ctactgggac gcggaaaagg
     1981 ccgagcgcag gctattgtct gccaacgcgg agtatttcta ccagaaatct ttgcgggata
     2041 acccggagat cgcacaggcg gccagcaacc ccatttcccg gttctggcag aaacagcaga
     2101 tcaagcgcca gtacgccaag gccgcccgga cagcgggcca gaccgcaggc gcggcggcac
     2161 agggcgcggc caagacagcc ggggcctcca cgaaagcggc caaggccgca gccaaagaaa
     2221 gtgaacgggc ggcctccttt gtggcccgcc actggaaagg ggcgctggcc gtccttgtgt
     2281 tggggctttt gatgctgctt atcatcggcg gcttacagtc ctgcaccgcc atgttcggca
     2341 gcgccggaag cggcatcacg gcgtcctctt acttttccga ggatagcgat atgttggccg
     2401 ccgaggacgc ctacgcggag ctggaagccg agctgcaaga ctatctggac aactacgagg
     2461 ccacccatga ctatgacgaa taccattttg accttgacga gatcagccat gacccctatg
     2521 tgctgatttc cattctgtcg gcgctccatg acgggatatt taccatcggg gaggtacagg
     2581 gtgatttgtc catgctcttt gacaagcaat atatcctgac agaaaacgtc gtggtagaaa
     2641 cccgttaccg caccgaaacc gatacatgga cggacgcgga gggcaacacc catacagaca
     2701 cctaccaagt gccgtatgac tattacatct gctacgtcac gctggaaaac ttcgacctgt
     2761 cccatgtgcc agtctatatc atggacgagg aaacgctaag tttctattcc ctgtatatgt
     2821 ccacgctagg aaaccggcct gatctgtttt ccggcagcca gtaccccaac gcctccaccc
     2881 tgacgccgcc gacctactac gacgtacccc cggaggcttt ggaggatgcc acctttgccg
     2941 ccatgctcaa agaggcggaa aaatatatcg gttatcccta tgtgtggggc ggcagctctc
     3001 ccagcaccag ctttgattgc agcggcttca tttcgtgggt aatcaatcat agtggatgga
     3061 acgtgggacg gctgggggcg cagggcctat ataacatctg cacccctgtt tccagttcgc
     3121 aggccaagcc cggcgacctt gtatttttca agggaaccta cgacaccccc ggcgtgtccc
     3181 acgtcggcct gtatgtgggc aacaatgtga tgctgcattg cgggaacccg atttcctaca
     3241 cgaaccttac ttcaaactac tggcaatccc atttttacag ttacgggcgt ttaccttaac
     3301 gccagaaagg agctttcaat tatggcaacc aaacttgacc gcatcgaaaa ggacatccag
     3361 aaaaccaagt ctaagatcgc ggagtttcag aaacagcttc gggagctgga aacgcagaaa
     3421 accgaacagg agaatttgca gatcatccag cttgtgcgcg gcatgaacat gaagccggag
     3481 gaatttgccg cgttcttacg cagcggcgca atgcaggccg cccctgccgc aaccccttac
     3541 catgaacagg aggacaatgc ccatgaagaa taagaccaga cggatttccc gtattctccc
     3601 cgcacttctg gccgtgatgc tctgcgtcac ggcctttccc gttaccgccc ttgcaggcgg
     3661 caatgacccg gcaccggccc cgctgccgga ggctacaacc gaacccgcta cgggcggttt
     3721 ggagcctgaa acagacgaaa ccggcaccgc cacggacggg acagaaaccg aggccgaaaa
     3781 agataccggc cctctgaccg gcaaggacat caccgacctg ttttccgcgc tgttcggttc
     3841 caaggtgagc attgccgcca cggacgacgg catccagatc accaccggca ccgacaaaaa
     3901 agaacctgaa cagaccggca ccgtcaccac caacggcggc aggctgaatg tccgcaccgg
     3961 cgcggggctt gacaagaccg cgttcaccca gcttcccaat gggacgacgg ttgaggtagt
     4021 cggcacagac ggcgactgga ttaaaattct cttgccggag cgcatcggct atgtccattc
     4081 cgactatatg accgtcagcg aaaaggaagt agcggctacc ggcgagggcg gtttttccct
     4141 ctccattgac ccggaggaaa ttgcttctct gctggaactg ttcaatggcg gcggggccag
     4201 cgccgccctg tccccggacg ggaacctgtc actgattgac gacatcggca gcgccgcgaa
     4261 aaatggcaag cagtttatca ccgtggaaac caaaaacggc aatgtgtttt atctgattat
     4321 tgaccgggac gacgagggaa aagaaactgt ccatttctta aatcaggtgg atgaagccga
     4381 tctgctgacg ctgatggagg acggggaaac cgtcaccccc gctgctgtct gctcctgtac
     4441 cacgaaatgc aaggccgggg ctgtgaacac gaattgcccg gtatgtaaaa ccaacctgac
     4501 cgagtgcagc ggcccggagc cgcaggaacc ccagccggag gaacccgaag ccccggagga
     4561 agaacccaag ggcggcgcgg gcggcctgat cgtgttcctg ctggtggcgc tggcgggcgg
     4621 cggtgcggcc ctttactatt tcaagttcaa aaagcccaag gctgaaacca ccggcagcga
     4681 cgatttgggc gagtatgatt ttggggagga cgaggatttg gaggacgagg aacccgaaac
     4741 cgagtttgac ccggaagctg attttgtgag tgaacaggag gacgaggaat gacatataaa
     4801 cttgtaatcg cggaaaaacc cagcgtggcc cgcagcattg cgggcgtgat cggggccacg
     4861 gagaaacacg acggctattt ggaggggaac ggctatctgg tgagctggtg catcggacat
     4921 ctggtgtcct ttgccgacgc cggacgctat gacgagcgtt tcaaaaaatg gcgctatgag
     4981 gatttgccca ttctcccgga aacatggcag tacatcgtcc ccgacgacaa gaaacagcag
     5041 tttgacgttc tctgttcgct gatgcagcgc cccgacgtga ccggccttgt gtgcgccacc
     5101 gacgccgggc gcgaggggga attgattttc cgtttcgtct accagatggc gggatgccaa
     5161 aagccctttg agcgcctttg gatttcctct atggaggaca gtgctattaa ggacggcttt
     5221 gcccacctga aaccgggcgg cgactatgat ccgctgtatc aatcggccct gtgccgggcc
     5281 aaggccgact ggctgatcgg cattaacgcc acccggcttt tttccgtcct gtaccacaag
     5341 aacctgactg tgggccgcgt ccagacgccg accttaaaaa tgctggtgga cagggacgcc
     5401 aagattaccg atttcaagaa agagaaatat cacatcgtcc atatcacggc gggcggcgcg
     5461 gacgcggtaa gcagccgctt ttccgacgct gccgaggcaa acaccgtcaa ggcggcttgt
     5521 gcaaaagcgc aggccgtttg cgtttccgtg acgcgggaga aaaagacgga acagccgccc
     5581 aagctctatg accttaccac cttgcagcgg gaggctaacc ggcttttcgg ctataccgcc
     5641 aagcagaccc ttgactatgc ccaggcactg tatctaaaga agctgctgac ctatccgaga
     5701 acggatagtc aatatcttac cgacgatatg ctgcccgccg tggaaagcct tgtgtccggc
     5761 ctgtgggagc tggtgccgtt tgccaagggc ctgaaccttt ccccgcagtt tgaccgcatc
     5821 ctgaacagca aaaaggtgtc cgaccaccac gccattatcc ccaccgctga atttgtgaag
     5881 cagggctttg gcactctggc cgagagtgaa aaaaagctgc tgtccctgat ctgctgcaag
     5941 ctcttatgtg ccgtggccgg gccgcatatc tatgaggccg tcaccgccac atttacctgt
     6001 gccgggcagg agtttaccgc caagggcaag accgtccttt cccccggctg gaaagagatt
     6061 gaacgccggt tccgttcttc cttaaagacc gacgccgacg aggacgccga ggcggtgcgg
     6121 gagctgccgg agctttgcga gggccagacc tttgcagacg tggccgcctc tgtcaccgag
     6181 catttcacca cgccgcccaa gccctacacc gaagatacgc ttttgtccgc tatggaacgg
     6241 gccggggccg aggatatgcc ggaggatgcc gagcgcaagg gattaggcac accggccacc
     6301 cgcgccgcca ttctggaaaa gctggtgcag atgggctttg tccagagaaa aggcaagcag
     6361 ctcattccca ccaaggacgg catcaatctg gctgtggtgc tgccggaggc gcttacttct
     6421 ccccagctta ctgccgaatg ggaaaaccgg ctgaccgaga tcgccaaggg gcaggccgat
     6481 cctgacgaat ttatggacgg catcgagacg caggcgcggg agctggtgca gacctattcc
     6541 tgtatcagtg aggaaaagca gaaattgttt caggccgagc gtgttcctat cgggacgtgt
     6601 ccccgctgtg gtgaggctgt ctatgagggc aagaaaaact actactgcgg aaaccgggcc
     6661 tgccagttcg tcatgtggaa gaatgaccga ttttttgagg aacgcaagaa agcgttcacc
     6721 ccgaagatcg ccgctgcgct gctcaaaacc ggcaaagtca aggtaaaggg cctctattcc
     6781 gtaaagaccg gcaaaaccta cgacgggacg gtgcttttgg ctgataccgg gggcaagtac
     6841 gtcaactacc gcattgaaca caggagctaa aggacagtaa cacagcaagc atagggagtg
     6901 tggctacact cccggtaaaa tcccccggcc gcgtccctgc cgggccgggg atttttgctt
     6961 gttggggaag tccccaaccc cctatttttt gaaacagaac agagaaagga gtgaccgcgt
     7021 gaacgaagta ttttctatcc aagtccataa ccggcagttt ttggaacagg gcaaacaagg
     7081 cgtctggctg gatttgccca ccacaaccga aacgctgcaa gccgccctgc gggatgttgg
     7141 cattactgcc gacaatccgc aggatttttt tatcaacggg tattcctgcc cggaggacag
     7201 acaccttgcc attccctatg acatggtgct ggccgccgac gtggacgagc tgaatttcct
     7261 tgccgcccgg ctgggccagc ttgacgccgc cgagatcgca gagctgaacg ccgccctgca
     7321 aaatccccgt ggcggctttg agaatatcgg ccagatcatc gactacaccg aaaatgtgga
     7381 ttactatgtg catcttcccg acgttagggg ggatggacag ttgggcgact actacctgaa
     7441 ccgttccggc atggtggata tgccggagga atggaaagcc gggattgata cggcgcagtt
     7501 tggacgccac attgcccagc aggaacaagg cgtttttacc cagtacggct atctggtgag
     7561 aagcggcgac gaatggcagc gcgtccatga gggccagccg gtgccggagg aataccgggt
     7621 gatgcactac ccccagccgg aagctgaccg ggaggaaccg caggcccagc agccggaggc
     7681 atccgaaagc cccgacaaag cgccgccccc ggttatcccg atcatgctgg acggcatgaa
     7741 cagcaaagaa cgcatgaaag tgattactga ccgtctggaa accggcgtac aggagctttt
     7801 tgaaagtagc cgctatatgg cttacttatc cgctatgtcc aagatgcacg attacagtct
     7861 gaacaatacg ctgctgatcg ccatgcaggg cggttcgatt gtggcgggct ataacaggtg
     7921 gcaggatgaa tttgaccgcc atgtgaagcg gggcgagaaa ggcattaaga tactggcccc
     7981 ggccccgttc aaggttaagc agcagatgga gaaaatcgac ccggccaccc agcagcccgt
     8041 gatcggcgcg gacggcaagc ccgtgaccga agaacaggaa gtgacggttc cggcctttaa
     8101 ggtggtgaca gtctttgaca tcagccagac cgagggcaag gattttccca tgccctatat
     8161 cagtgaactt accggcgacg tgcagcagta tcaggatttc tttgccgcgc tggaacggac
     8221 atcaccctat cccataagca ttgagccgat ttcagacggg gccaagggcc gctgcttcta
     8281 cgcaaaacag cgcattgcca tcaacgaggg catgagcgaa ttgcagaccc taaagaccgc
     8341 catccatgag attgcccatg ccaagctcca cgccattgac cgggaggccc ccgccgagga
     8401 acaggcggcc cggccagacc gcagcacccg cgaggtacag gccgaaagcg tggcctatac
     8461 cgtctgccag tattacgggc tggacacgtc ggactattct tttggctatg tggccgattg
     8521 gagcagcggc agggagttat ccgagctgaa agcctccctt gaaaccatcc gggccacggc
     8581 cagcgaattg ataaccgaga tcgacggcca ttttgcccag cttcagcagg agcgcgaggc
     8641 ccggcaggaa caggaggccg tcacggttga cgttaccccg gaggcagcgg ccccggcgca
     8701 ggccgaggaa gaacaggccc cggcccttga tccggcagcc gagccgcttg tgactgtgct
     8761 atggagcgaa agcccccagc taaaggacgg ccagcaaatg ccgttgcatg aggccgacgc
     8821 gctgtttgcc cgtctggatg cagcatatcc ggcagaggca ggctatgaca aaaccgcgtt
     8881 ccgcattgac tgtatctttc agggcgagcc ggacagctac gaggggcggc aggattttgg
     8941 agatagggac ggttccctga tcgaacatat ccagagctac caccaatttt acgcgcagga
     9001 cgaaaactgg aaaaagcatg taatcgctac aaccggcata gagggctggg agcaggacaa
     9061 ggcccatcgg gatatgctgt taggcgagtt tgtcccctat atgaagctgc attgcagcct
     9121 tgcccaaatg gaacagacgg cgcagggccg cttacagtcc ggggaaaccc tgacgccgga
     9181 ggaaacggcc tatttggggg cgcttctctc ttatgtgcag gagtgccgcc ccatgctcaa
     9241 tcagggccag tatcagttgc cggagccgcc gaaactggct gacttcgacc agagcttgca
     9301 ggactacaaa gcacaggtac aggccgagat cgcgcaggag gccgccgcag ccggtatgac
     9361 cgtggaggaa tacgcggcgg ctggctatga agcaccccaa caggacacct tttccatcta
     9421 ccagcttcga ggcggggagg aaacccgcga ctatcggttt gagccatacg agcgtttaca
     9481 ggccgcaggg ctggccgtgg acagtgccaa ctatgacctt gtttatacgg caccgctgga
     9541 taaaagcacg tctttggagg atatttaccg cacattcaac atcgaccacc cggcagattt
     9601 taggggacac agcctttccg tttccgacgt ggtggtgctg caccagaatg gacaggacac
     9661 cgcccactat gtagacagct tcggctatat ggaagtgccg gagtttttgc gggaaaatcc
     9721 cctgcgtacc gccgaactgt ctacggagca gaacgaaaac atgattgacg gcattatcaa
     9781 caatacgccg cccgaaccgc agccggagga acccgaacag ctatcttttg aggatgtggc
     9841 agttgcttcc ggccagacac aggaggaacc cccggaggct gcgcccccgc aaacggctgt
     9901 ccgctattat cccatcaatg agggagccgc ccgccgcgcc aaggaagcca tgagcttttc
     9961 tgactaccgg cccggcagcg ccacggcgga ataccggcac tatgtcgatc aggccgtgga
    10021 aattgcggag cgccagaaaa agcgtgtgga gccagagtat cacgaaaaga tagacagcct
    10081 ccttgacacc tacgcccgca agctggccga gaacatgaat aagggctatg agattaccgc
    10141 ccgcgtcccc tctgtcctga ttgccgggcc gtccaatttc ccggtacggg cgaaagaaaa
    10201 gcagaacgcc gccagtgacc gcaacatgga ggaatttcag tacattcagg gcttgcttga
    10261 caaaatccgc agcaccggca tgggtggtat cagcgccgac gatccgcagg ccgtttccaa
    10321 gctggaaaag aaactggaaa agctggaagc gtcgcaggag ttgatgaagg ccgtcaatgc
    10381 ctactaccgc aagcatggga cgctggacgg atgcccccat ctgaccgaaa gaggcattga
    10441 aaacctgaaa gcggatatgg cgtcgggctg gcactatgag aaaaaaccgt tccagtcctg
    10501 gcagctctcc aacaataacg ccgagatacg ccggttaaag ggccgcattg aggaattgac
    10561 ccgccaaaag gaggcggcct atgtgggatg ggagtttgac ggcggcactg tggagatcaa
    10621 ccgggtggcc aaccgcctgc aaatcttctt tgaggggaag ccggatgctg ctgtacgcga
    10681 cgagctgaaa agcaacggtt tccgctggtc gcccaaggcc ggggcatggc agcggcagtt
    10741 aaacgacacc acaatccgcg tcgctgaccg tatcaagtgc atccagcccc tttccggcga
    10801 aaagcccagc gccttacagc ttgcggcccg cagggagaaa gaaaagccct ctatccgggc
    10861 gcagctcaac gcggccaaga ccgaacaggc caagcagcca ccggcgcggg aaaaatcaaa
    10921 gggattggag gtagagtaaa tgcagaagtt tgaaaatgtg gacgttctgg ccgcgctgga
    10981 acagatcatg cggcagaaca ccgcgtttta tcaaagtgac tttggcattg acaaagacat
    11041 tatcaggcgg gatgccgcca gcgatcaggc cgcagacaag accctgctct ggatgtcccg
    11101 gccctccggg acgtactgtt tccgggaacg tgatgttttc ctgaaagaca cccgacagta
    11161 caacacatgg aaattttacg gggagcagac ccgtgacaag atactggcct acgcggtgga
    11221 gctgaccggc acacagggcg gcactatccg gggcaatctc tatgagctgg actatccgca
    11281 gcatttccgg cacgtcattg aaaccgccca gccggtgagt gtgaaccggc tcttttatga
    11341 gcatggcacc cgtgacatcc cggagagcca gcgttttgac ggaagccctg accgggtatt
    11401 gggaaacttt ttgtactatg aggcccagcc ccatgacccg gctgtgctgc aggaggcgct
    11461 acggacggag cagcacagcc gggggcgggc cgtccccggc gatttcaaag tccacgtcac
    11521 cgctttgcat gacagccgga ttgaaaccga ggcccggcgt attgtggaca gcctgaaaga
    11581 tctgtccgcg cctaacagcc ccaataaaac gcatttcatg gtggagatct ccccgcactt
    11641 tgcggcgatt gcttccacca aggataatga ccggctcttt gccatgctgc cctataaatc
    11701 cctgtgtttt accgggatga aaggcaggca tggcgtgtat gccgtgatta accgggacga
    11761 gcgccgggat gtgagtattc gcaggccccg gccctctatc cgcaagcagc tatccgacag
    11821 caaacaggcc gccagcccaa aaaaggcggc ggccagaacc aaaaagaatg aattggaggt
    11881 ataaggaatg atgcacttta ccgtggagga agaaaacctg atctgtatgt accacaatgc
    11941 agaccggcgc agaacgataa acaggctcac cgccgtcatg ccggttatgg atggggatat
    12001 gcgggcgcag gcccagcaga ccctttccaa gctggaacgc atgacggatg ccgactatga
    12061 cggccagaag ttccacttca cagacgaata acgaacagca gacgccgggc gcggcggcca
    12121 tgtggtcgct tgcgtccggc gtcttttctt tttgcctatt cctacgcatt tagaacccgg
    12181 tattgtcggg gataggatga agtattcgct tgcccctgca aacatgcggg agaagccttg
    12241 cagcgggcgg ttttcaatcc cttaaatgcg taggttgcag ctataattag acccctgaaa
    12301 cagatatagc cgcgcagcgg gtgtatctgt ttcaggggtg tgcaggtgat agccgcaaag
    12361 cggcgtaaga gggtgcagcc ctcttgccgt ctatctctgt tccagcggga agctcggcaa
    12421 tgcttccagc agtttcaggg aaatgcgctg ccgtatatct tcgtcaatga ctttcttctt
    12481 tttgccgttt gcctgtctga tctccaccgt ggaaagctcg ttgatgtagt cagcataatg
    12541 ggccaacact ttttccgtgg cccacctctc accggcaacg gcggctttga tcgtttcata
    12601 gtccagcagc ttttcttgtc cgtccatcct gatacccccg tcagactgat tttttctatg
    12661 ataccatatt cggaagcatg gaaccagaga aaacggcgct tttctgctgg taaattctac
    12721 gcatttagaa cagcacttcg gcggggatag gataaaacac ccacataccc cgctgaccat
    12781 gccggaaaag ccttgaaata taagggcttt tacagctcta agtgcgtagg tttccagtat
    12841 cttttccgct ggcgctcggc ctctttgcgg cggcgcatcc gtttggagca ggccgcgcag
    12901 tatttggccc ggttagactt cggcgtgaac gtggccccgc actcacagca gcgtttcact
    12961 tcgtcccggt ctttggtgat ctcggcatac agggccgcat ccagcgggag gacagcggcc
    13021 ttaaaccagc ggcacagaag cgaataggaa atgctctgta cgcaaataca ttcttcgccg
    13081 tcgtccagca acaggcagtt gccgtcacaa tagttgcagc actcatgcgt cagcttccgg
    13141 gccttgcgat attgagggta attcattcgt ggaatactca tagctcttgc gtccattcct
    13201 ttcccggcgt catacgcaaa atgccgtcca cattctgctt gatgatgccg tattcctgca
    13261 tctgcttttt cgcttcgccg tacttctcat acagccgttc cttttcctcg gccaatcgct
    13321 tgtactcggc tttcagggcg tacagatcgg ggagcttttt cacaccctgt ccctttaggg
    13381 cctttgccgc agcttcatac aaaatcaacg tgctttcatg ctcacgccgg aattttgcct
    13441 tatccgctgc attgcggtat tccatagcca cgggccagag ctgcttgtag gtagaaatat
    13501 tctttatcag caccgccata tccgcaagcc ggtgttccat agctttgagg gtagcggcgg
    13561 cctcgtcgct ggcggcggtg atctcggcca ctttgctttc caaatccggg taggtgtcta
    13621 tcccatgttc agtcaggaaa ttcaaagtct ttgcggcctg cttcaaattg tggagcttcg
    13681 cccacctctc atagcctgcc gactgctggg ccttaatgtt gttttccaga tcaatccgca
    13741 aagaaatccc cttgcgctct ttgggcgctc tggtgataac gcggcctttg atacgctctt
    13801 tgatggcttc ctcggtgtag gcttccccca atgtcttgca gcgggtaaag cgttcctgtc
    13861 cgggagcgcg gaaagaaata aactttcccg gcttgatctc atagccctgc gcctgcatga
    13921 gccgcaggaa atcatcaaaa tcttttgcct gcgggatagc ggcgtcaatg gctgctttga
    13981 gcttcgcttt ccagctcgtc cctttccgct gggcgtccca ctcggcatag ctcttgccct
    14041 tactctggcc gggcttcacc acggacaatc cgttttcctt gcacagcctg tcgctggtgc
    14101 gccggatata ggcatagctc tgtttgttgg aaatgtactt gcgctgattt accatatcca
    14161 cggcacagaa aataatgtga ttgtggacgt ggcccttatc aatatgggta gtcagcacat
    14221 agggatattt cccttgcagc acttcctcgg caagctgctt cccgatctca tgggcctgtt
    14281 ccggcgtggc ttcgccgggg gcaaaggatt gtatcaggtg gtggcccaga ttattgccct
    14341 tttggtatgc ctgcgccagc agcatttcaa attcaatgtc cgctgtttca taagagcagg
    14401 caaaggaggc caccagcagc ttgccgtcag ttttgtccgg gttctcgatg tagtcaaggg
    14461 cttttttcag cgttgacttg ataggatgta tctttgtaac tgccatatct gcgccagcgc
    14521 cccctttata tcctcgatgt cctgtgcgta caccggcccc ataccgttca gcctctttgc
    14581 aatctgattg acgttgacgc cgatcttctg tatctcggcg gtctgcgccc ggatgtcgct
    14641 ggtgtccatg ttgacgatat agccgtcaat gagcatcttc cgggcgtagg ctgaaaagtt
    14701 cttcgtatgc agcagcgcca ttttctgttc aatcatagcc cgttcctccg gcgtcacagg
    14761 gacgcgcagc acgatttttc gctttctgtt tgccatgctt caccgtcctt tccattcagg
    14821 ggtttgggga tttccccaac aagcaaaacg gacgcggccc ggcagggacg gggcaggccg
    14881 tttttccggg agtggctaca ctccctatgc ttgctacgtt actgtttctc cactaacaag
    14941 acgtttccaa agccccaaac tacccgcaaa atagccctct ggaaagaaaa aagtgcaaaa
    15001 aaatagaagc tacgctgtgc agctcccgct aaaagtaaaa cagaggatgc cagttacaca
    15061 tcctccgttt cattcttctt cggcttttca attagtatgg cgctgtcaat cgcgccctcg
    15121 atgatcggca ggtattcttc ggggcagagt ttcagcttgt ggctgaccct ttgccgcagt
    15181 tcgctttcct cccgcatgat ctccgggtta aagtagcgtt ccacaggaag cccgcaggct
    15241 ttaataagct gtatgacaac cggcagactg ggaatggccc cgtcgttttc gatgttggca
    15301 agatagcgcc agtcaatatt tattttctct gccattgctt tgcgcgaaac gcgctttgct
    15361 tgccgggccg ctttcacatc cgcgccgaaa gtttcaaagc cgggacaatc ttcaatattc
    15421 gccatatcgc acctcccact tacattgtat atttcatact ctgcccgtgg aatgttggta
    15481 tatgcacctt tcttaaagtt tatatttcac attttgtttc ttttattctt cgagcaaaag
    15541 aattataatg taccctgtgg aggtgtacta tggactacat gacgttgaaa gaggcaggcg
    15601 agaaatgggg tgtgccccca cgatggataa actactattg tgctgcggga cgcatccccg
    15661 gcgctgtaaa gatggcgacg atctggctca ttcccaaaac tgcggaaaag ccgattgaca
    15721 ggcgtacaaa gaaaggaaag gcggttactg aatagtataa tgaaaccggc gcattttgga
    15781 tatggttttg aagagagaga aagcatggta ttttgtcacg gaaaaactat aagatgatag
    15841 atggcaggct gctccagacc gataaaaagt attctcatct gaaacagaaa gaaaagattt
    15901 acacatggat gtatgaagaa gcgaaacttt atcatgacag aaatggaaaa tgcccgaaac
    15961 aaagacgagg aagctgctgc tgtatcagcc gtatatggta gaattgaaaa ggccggcatc
    16021 tggattcttt atggtgaagt ctccagacat tggataactg ctggtctaat gcgcctttga
    16081 gtatggggtg attgatttta ataccgggga ggtacggctt tttgaggaaa atattctggg
    16141 atttacattt ggcgattatt acatttcctg taactgcaag gacacggatg atgaagatat
    16201 gaggaatata gacttgctga ttttctactg tcttgaacgg tattaggact gaatctgctg
    16261 cgcggagtgc ggcaagagcg accggaggga gtacacacag gccctgcctg tgtgaaaagc
    16321 cataccgcaa ggtgcattcg caaaaaagag gccgcgcatt ttgctgaggc ttggggtgtc
    16381 cccaacaagc aaaacgagaa cgccgcaggc gggcaagtgg tggatcagaa tatccgtaaa
    16441 ggatattctg atccattgct tgccagtatt atcgggatgt tttgacaacc gctgtaatcg
    16501 aaacatccca aacattaaga aggtgttaat tattattatc ttgatttaat tcgtttaaac
    16561 caataatata gatacataca ccatgaaaag aagcgagtat cccaatgacg gatgattaaa
    16621 atccgaaaag aaatcgcttt gaaactacag ggaaactaca gactgttatg ttatcttctt
    16681 aaaatggagg gatttttatg tcgatacgaa ttctacttgt cgaggatgat gatcatatct
    16741 gtaatacagt aagggcgttt ctggctgagg caggatatca ggtggatgcc tgcacagatg
    16801 gaaatgaggc atacaccaag ttttacgaaa acacttatca actggttatt cttgatatta
    16861 tgctgcccgg tatgaacggg catgaacttt tgcgtgaatt tcgtgcgaaa aatgatactc
    16921 ccattctgat gatgacagcc ctgtcggatg acgaaaacca aatccgggcg tttgatgcag
    16981 aggcagacga ctatgtaaca aagccattca agatgcagat tttactaaag cgggtggaag
    17041 ccctgctgcg gcgcagcggt gcgctggcaa aggaaatccg tgtcggcagg ctgacacttc
    17101 tgccggagga tttcacggta ctttgtgacg gtacagagct gcccctgaca cgaaaagaat
    17161 ttgaaatcct tttgctgctg gtgcagaaca aaggcagaac cttaacgcat gaaatcattt
    17221 tgtcacgtat atggggatat gactttgagg gtgatggcag cacagtccac actcatatca
    17281 aaaatttgcg ggccaagctg ccggaaaata tcatcaaaac cattcgcggt gtaggatacc
    17341 gattggagga atcataataa tggaaagaaa agggattttc attaaggttt tttcctatac
    17401 gatcattgtc ctgctactgg ttgtcggtgt aacagcaaca ctgtttgcac agcaatttgt
    17461 gtcttatttc agagtgatgg aattacagca aacagtaaaa tcctatcagc cattggtgga
    17521 actgattcag aatagcgata ggcttgatat tcaagaggtg gcagggctgt ttcactacaa
    17581 taaccaatcc tttgagtttt atattgaaga taaagaggga agcgtactct atgccacacc
    17641 aaatgccaat acatcaaata gttttagacc cgactttctt tatgtggtac atagagatga
    17701 taatatttcg attgttgctc aaaacaaggc aggtgtggga ttgctttatc aagggctgac
    17761 aattcgggga attgttatga ttgcgataat ggttgtattc agccttttat gcgcgtatat
    17821 ctttgcgcgg caaatgacaa cgccgatcaa agccttagcg gacagtgcga ataaaatggc
    17881 aaacctgaaa gatgtaccgc cgccgctgga gcgaaaggat gagcttggcg cactggctca
    17941 tgatatgcat tccatgtatg tcaggctgaa agaaaccatc gcaaggctgg aggatgaaat
    18001 cgcaagggaa catgagttgg aggaaacaca gcgatatttc tttgcggcag cttctcatga
    18061 gctaaaaacg cccatcgcgg ctacaagcgt tctgttggag ggaatgcttg gaaatatcgg
    18121 tgactacaaa gatcattcta agtatctgcg cgaatgcatc aaaatgatgg ataggcaggg
    18181 caaaatcatt tccgaaatac tggagcttgt cagcctgaat gatgggagaa tcgtacccat
    18241 agctgaaccg ttggacatag ggcgcacggt tgccgagttg ctgcccgatt ttcaaacctt
    18301 ggcagaggca aacaaccagc ggttcgtcac agatattcca gccgggcaaa ttgtcctgtc
    18361 cgatccgagg ctgctccaaa aggcactatc caatgtcata ttgaatgcag ttcagaacac
    18421 gccgcaggga ggcgaggtac ggatatggag tgagcctggt gctgaaaaat gccgcctttt
    18481 tgttttgaac atgggcgttc acattgatga tactgcgctt ccaaggctgt tcaccccatt
    18541 ctatcgcatt gatcaggcgc gaagcagaaa aagtgggcga agcggtttag gacttgccat
    18601 cgtacaaaaa acgctggatg ccatgagcct ccagtatgcg ctggaaaaca cctcggatgg
    18661 cgttttgttc tggctggatt taccgctcac atcaacattg taaatattta aaacttaaat
    18721 gattttgacc gacaggtata accctgccgg tctttttttg ctgctacagg aaaactacag
    18781 actggctaca gggaaagtac agatgcgctt gccataataa caatcgtacc agccacaaat
    18841 cgtagtttta ttgcaaagga ggcattcgat caaatggaaa aaagcaacta tcattccaat
    18901 gcggatcatc acaaacggca tatgaaacaa tccgtggaaa agcgggcttt tctatgtgcg
    18961 ttcattattt cgttcacagt ctgcacgctg tttttggggt ggagactggc ttccgtattg
    19021 gaggcaacac agataccgcc catccctgca actcatacag gcagcagcac tgacgtagtg
    19081 gagaatttgg aggaaaacgc tcttgccacc gccaaagaac agggagatga acaggaatgg
    19141 agcctgattt tagtgaacag gcagaacccc atccccgcac agtacgatgt ggagcttgag
    19201 caactatcaa atggtgagcg gatagatatt cggatttctc cctatcttca agatttgttt
    19261 gatgccgcaa gaactgatgg agtttacccg attgtcgcat ccggataccg aacaacagaa
    19321 aaacagcaag aaattatgga tgaaaaaatt gccgaatata aggcgaaagg ctacacctct
    19381 gcacaggcta aagcggaagc agaaacttgg gtggccgtgc cgggaacgag cgagcatcag
    19441 cttggtcttg ctgtggatat caatgcggac ggaattcatt caacaggcaa cgaggtttat
    19501 agatggctgg atgaaaacag ctatcgtttt ggttttattc gtcgctaccc accagacaag
    19561 acagagataa ccggtgtgag caacgagccg tggcattacc gatatgttgg catcgaagct
    19621 gccacagaga tgtacaacca aggggtttgc cttgaggaat atttaaaacc agaaaaatga
    19681 gaaaaggatg ttatgttatg gacagaaaaa gattgacaca gcgtttcccg ttcctgcttc
    19741 caatgagacg ggcgcagaga aaaatgtgct tttatgcggg aatgagattt gacggctgtc
    19801 gctatgcgca gacgataggg gaaaatcgct ttcccatttg ctctttgaaa cggattgcgc
    19861 attatacaac cacaataccg gatttgacat gatataccag gaaaacaagg tgttcaactt
    19921 aaagctggca gcaaagacct taaacggctt attgataaga ccgggggaaa ccttttcttt
    19981 ctggtggctg gtacgccatg cggacaaaga taccccctat aaagacggcc ttacggtgac
    20041 caatggtaaa ctcaccacca tgtcgggcgg cggtatgtgc cagatgagca atttactatt
    20101 ttggatgttc ctgcatacgc cattgacaat tatccagcgc agaggtcacg aagtaaagga
    20161 gtttccagag ccaaacagtg acgagattaa aggggtggat gcaaccattc cagagggctg
    20221 gattgattta aaagtgcgaa acgataccga ctgcacctac caaatatggg tgaccttaga
    20281 tgatgagaaa atcatcggtc aggtgtccgc cgacaaagag ccccaagcat tatacaaaat
    20341 tacaaatggc agtatccagt atgtccgtga aagtggcggg atttatgaat atgcccaggt
    20401 taaacggatg caagttgcct taggtaccgg ggaaataata gattgcaagc tgctttatac
    20461 aaacaaatgc aaaatctgtt atcccctccc ggaaagtgtg gatattcagg aggagaacca
    20521 atgagaaaaa gtgtgggcat tactgttttt ggatgcgaac aggatgaggc aaatgctttc
    20581 cgcgctttat cgccggattt tcatattatc cctacgctga ttagcgatgc gatatcggca
    20641 gacaacgcaa aattggccgc tggcaatcaa tgcgttagcg taggccataa gtccgaggtt
    20701 tccgaggcga caattcttgc gctgagaaag gtcggggtaa aatacatttc tacccgcagc
    20761 atcggctgcg atcacattga tacgactgcc gccgagagaa tgggaatctc ggttggcacg
    20821 gttgcgtatt cgccggacag cgttgcggat tatgctttga tgctgatgct gatggccata
    20881 cggggtgcaa aacccaccat gcacgccgtg gcgcaacaag atttcagatt ggatcgtatc
    20941 cgggggaaag aactggggga tatgactgtg ggagttattg gaaccggcca tatcgggcaa
    21001 gcggtcgtca aaaggctgcg gggatttgga tgccatgtgc tggcctatga taacagccga
    21061 aaaatggatg cagattatgt ccagcttgat gagcttctaa aaaacagcga tattgttacg
    21121 ctccatgtgc cgctttgtgc ggatacccgc catctgatcg gtcagaagca aattggagag
    21181 atgaagcaag gcgcattttt aatcaacacc gcgcgcggag cgcttgtcga taccggggcg
    21241 ctggtggagg cgttggaaag cggaaagctg ggtggtgcgg cactggatgt gttggagggc
    21301 gaggatcagt ttgtttatac cgattgctcg cagaaagtgc ttgaccatcc ctttttgtcg
    21361 cagcttttaa ggatgccgaa tgtgatcatc acaccccata cggcgtacta cactgagcgt
    21421 gtgctgcagg atactacaga aaaaacaatc aggaattgtc tcaattttga aaggagttta
    21481 cagcatgaat agaataaaag tcgcaatcat cttcggcggt tgctcggagg aacatgatgt
    21541 gtcggtaaaa tccgcaatag aaattgctgc gaacattgat acggaaaaat tcgatccgca
    21601 ctacatcgga attacaaaaa acggtgtatg gaagctatgc aagaagccat gtacggaatg
    21661 ggaagccgac agtctccccg ccatactctc cccggatagg aaaacgcatg ggctgcttgt
    21721 catgaaagaa agcgaatacg aaacacggcg tattgatgtg gctttcccgg ttttgcatgg
    21781 caaattcggg gaggatggtg cgatacaggg gctgtttgta ttgtctggta tcccctatgt
    21841 gggctgtgat attcaaagct ccgcagcttg catggacaaa tcactggcct acattcttac
    21901 aaaaaatgcg ggcatcgccg ttcccgaatt tcaaattatt gataaaggtg acaagccgga
    21961 ggcgggtgcg cttacctacc ctgtctttgt gaagccggca cggtcaggtt cgtcctttgg
    22021 cgtaaccaaa gtaaacggta cggaagaact taacgctgcg atagaagcgg caggacaata
    22081 tgatggaaaa atcttaattg agcaagcgat ttcgggctgt gaggtcgggt gtgcggtcat
    22141 ggggaacgag gatgatttga ttgtcggcga agtggatcaa atccggctga gccacggtat
    22201 cttccgcatc catcaggaaa acgagccgga aaaaggctca gaaaatgcga tgattacagt
    22261 tcccgcagac attccggtcg aggaacgaaa tcgggtgcag gaaacggcaa agaaagtata
    22321 tcgggtgctt ggatgcagag ggcttgcccg tgttgatctt tttttgcagg aggatggcgg
    22381 catcgttcta aatgaggtca ataccatgcc cggttttaca tcgtacagcc gctacccacg
    22441 tatggtggcc gccgcaggaa tcacgcttcc tgcactgatt gacagcctga ttacattggc
    22501 gttaaagagg tgataagtat ggaaaatggt tttttgtttt tagatgaaat gttgcatggc
    22561 gtccgttggg atgccaagta cgccacatgg gataacttca caggaaaacc ggtggatggg
    22621 tatgaggtga atcgcatcat cggcacaaag gccgtggcgt ttgctctgcg cgaggcacaa
    22681 atccatgcgg ctgcgcttgg ctatggcttg cttttatggg atggatatcg gccaagaact
    22741 gcggtggact gcttcctgcg ttgggcagcg caaccggagg acaatctcac aaaagaaaaa
    22801 ttttacccca atatagagcg agccgagttg attacaaagg gttatgtggc ctcacaatcc
    22861 agccatagcc gtggaagcgc aattgatctt acgctctacc acctggatac aggggaactt
    22921 gtttcaatgg gaagtaactt cgattttatg gacgaacggt cgcatcatac agcaaaaggg
    22981 ataggggatg cagaggcaca aaatcgaaga tgcttgcgta aaatcatgga aagcagcgga
    23041 tttcagtctt atcgctttga atggtggcac tataagttga ttgatgagcc ataccccgat
    23101 acctatttta attttgccgt ttcataatga aagtattggt tttgtatcag attttggatt
    23161 tgataaaatc tatgctggtc atggacaatc ttttactgga aagtggttaa agcatctttt
    23221 gtaatacatt gcaaattata gtattcaatc tgcccagcgg cagaaaagaa aaaaaccgct
    23281 gttgggcaga cctttcccac gcccattttg tattttgctg gcggcggttt tgagaaatca
    23341 aggccgccgt tcttttctgg cagcaaaagg tgtggcggcc cataggagga tgccgctatg
    23401 ctgttttgct attgccatgc tctaatccac ttccaccact caaaaaagcg gctctttgtc
    23461 aataaggact gatgagttgt gcaaaatc
//
LOCUS       DACDEG010000273        13057 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.guided.070, whole genome shotgun sequence.
ACCESSION   DACDEG010000273 DACDEG010000000
VERSION     DACDEG010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.guided.070"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(48..2006)
                     /locus_tag="IV142_002956"
     CDS             complement(48..2006)
                     /locus_tag="IV142_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8486512.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2003..2875)
                     /locus_tag="IV142_002957"
     CDS             complement(2003..2875)
                     /locus_tag="IV142_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8486513.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3217..4089)
                     /locus_tag="IV142_002958"
     CDS             complement(3217..4089)
                     /locus_tag="IV142_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8486514.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4217..4786
                     /locus_tag="IV142_002959"
     CDS             4217..4786
                     /locus_tag="IV142_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP8486515.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            4853..5590
                     /locus_tag="IV142_002960"
     CDS             4853..5590
                     /locus_tag="IV142_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8486516.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5821..7089
                     /locus_tag="IV142_002961"
     CDS             5821..7089
                     /locus_tag="IV142_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP8486517.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7576..8040
                     /locus_tag="IV142_002962"
     CDS             7576..8040
                     /locus_tag="IV142_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8486518.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8024..8653
                     /locus_tag="IV142_002963"
     CDS             8024..8653
                     /locus_tag="IV142_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8486519.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9007..10485
                     /gene="msr"
                     /locus_tag="IV142_002964"
     CDS             9007..10485
                     /gene="msr"
                     /locus_tag="IV142_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8486520.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10532..10978
                     /locus_tag="IV142_002965"
     CDS             10532..10978
                     /locus_tag="IV142_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP8486521.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11177..11692)
                     /locus_tag="IV142_002966"
     CDS             complement(11177..11692)
                     /locus_tag="IV142_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8486522.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            11809..13011
                     /locus_tag="IV142_002967"
     CDS             11809..13011
                     /locus_tag="IV142_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8486523.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4065 a         2402 c         2670 g         3920 t
ORIGIN      
        1 cagctcctgt ttgtttatcg ataaaaaagt ctggggaaga cgatggctca ggctttgttg
       61 gaaacagagc cgattttatc atttgctttt acatcacgtg cgggttcgat ttctaagctt
      121 tggactttgt cgccatttgt gaatacaacg accatatccg tgtttttgcc tgcagattga
      181 atagcggcaa gatcaactaa agcaatcaat tgacctcttg cgactttttg accttctttt
      241 acatataaag tgaatggttc gccttttaag tcaacagtgt tgatgcccat atgaagcaag
      301 acttcgatac cagcatctgt ttggattccg acagcatgtt tcgttgggaa aatgtttgtg
      361 atcgtaccag cgattggaga gaagatttct ccattttctg gtaatacagc atagccgtca
      421 cccatcattt tttccgcgaa tacatcatct gaaacttcag tgattggaat cagtttccca
      481 tttgcgatag cgtaaatatc ttgttcgatc gttccaccgg caaatgtttc tgttttaacc
      541 acggtgtttg tttgtgcagg tcgtgttgca gcaccaccat gtagttttgc catctcatct
      601 gcaacgaatt gtacttctgt acctacgatg acttgcgcat tttttccgtc gatgatcttc
      661 ataccaggta cgccagtggc tttgatttta ttttgatcga tcaggcctgt atctttgact
      721 tgcaaacgaa ggcgtgtagt acagttatcg atcgaagtta cattgtcggc tccgcctaat
      781 gcagcgtaga ttttgcttgc ttgtgccgca tatttattgt ctgaattagt cgttgcagta
      841 tcgtcatctg aaagatctgc ttcctcgcgg ccaggagtca tcagatggaa ttttttgata
      901 gcaaagttaa agccaaagta gtaaatcact gctgttacta atccttggac gatcaacatc
      961 aatggctgat tcgcaatcgg atttttcaaa gataggaagt aatcgacaaa tccggcacta
     1021 aaggcaaatc ctgctgtcca atggaagaat gcactcactg ctaaagaaat cccagtgaaa
     1081 attgcatgca agacatataa tggccaagca acgaacataa atgagaattc aagtggttct
     1141 gttacaccag taaagaatga agcaaaagct gctgctaaca tcaaggaagc tgtttttgct
     1201 ttgttttctg gacgtgcatt tctgtaaata gcatatgctc ctgctggaag cccgaacatc
     1261 atgattggga aaaatccagc ttgatacatt cctgttacgc cttttgttcc ttcagaagcc
     1321 aaaaatttac caatatcatt gattcctgct acgtcaaacc agaacacgga gttcattgct
     1381 tgatgcaatc ctgtagggat cagtaaacgg ttgaagaagc cataaagacc tgcaccaccc
     1441 cagcctaaac tcaaaatgaa tttagcaaaa gaaactaaag cagagtaaac cggaggccat
     1501 acgagcagta gcactgcact gatcagcaac atgacaaagg cagccaagat tggcactaga
     1561 cgcttgccgc taaagaaaga taaagccatc ggtaattttg cttcgtggaa acgattataa
     1621 agagctgccg caatcaatcc ggcaataata ccaataaata cgttactgat ctgagcaaaa
     1681 gatggatcaa ctgattctgg tttgatattc aataaagtag ctactgaatc agtttttaaa
     1741 acatttactg gaacttcaaa tgcgaccaaa cctgccaagg cagcagcacc tgatttgtcc
     1801 actgacatcc ctagtgctaa cccaacggca aacagcaaag gtaattgacc taaaactgcg
     1861 ttaccgccag cttgtaaaaa atgagcgaca atgtcgttac tataagatgc ccaccagttt
     1921 ccgatcccta ctagtaaagc agcagcaggt aagaccgcaa ctggaagttg aagggaacgt
     1981 cccattcgtt gtagatatgc tttcaatttt tttcctcctc atgattgtta acatttgttt
     2041 gtacgattgc gcttcttaaa cgctcgatat ggatggccaa gtaacccagt tcttctttcg
     2101 tgatcgcgag ctggtattct ttggtcagca acacacggat ttttttagaa atcttatagc
     2161 tttctgggta ttttcgtttg accatttcta cgatttccgt gtctaaaaca gcatattgtt
     2221 gcgcatgggt tcgttgtaga agcagtcgta aatgattcac cagtcttgaa taattcagtg
     2281 aaaatggttc tgaatggata tctgtttcca attctgattc cactaaatgg atgatgtcag
     2341 atacaatcgt cacttcacgg atactgcgat ggttattgtt ccgaccgctt cgtccgctgt
     2401 gaatatggat tgcgatatat cctgcttcgt cataagtata tggaatccca agctcttttg
     2461 tcagatactc tactgcccat tgagcaatac tgaattcatc actgtaaagc acttcgattt
     2521 ctcttaaaag tttgttgcgg atgacgatac cgttattaat gttctccgca gaaaaagcaa
     2581 tgtggtcggt caaagcgatc aatacatgtt cattcaactt ttccatcaaa acagtttccg
     2641 catgatcaat gatcgtttct gctgcaaata aaaagcgttc atcaatctga ttaagtaaat
     2701 tttgcaactt gatttgacct tctggttcca tgacaaatat ccgttcgaca tcacgatcaa
     2761 aaatcaaatc atttcttttt ctgtcaaatc caatcccttt tccaatagct actttttcct
     2821 gtccaccgtc atctaccaat actgcatttt gattaaagat ctttttgatt ttcatgcgcg
     2881 cacccttttt gaagatttgg ttttcacgtt tgctagacca gttgattgcg gccaaacaaa
     2941 aatagacacg aaaaacctcc tagggaaacc atgttcctca gagatttttc atgcctagta
     3001 tgatctagtc acatgaaacg aattattgtt aacgtaatca attttatata gacagttatg
     3061 ttttgtcaat tcttttttta taattatagt aatttactgg aattatcaga aataaaagga
     3121 aggaacaaat taagaaactt atgttattta atcattggat cttttgctta acttattcgt
     3181 tgttaaaaag ctgaaagcga aggaaaaaaa ctctacttaa ctgtatttct ccgttaggac
     3241 atctaaaaat ttacgagcag caggagaaag aggctggtct tttttccaaa tgagattcag
     3301 tgaagcttgt aatggcggat cgagtgggat gaatactttt tccgtcccat aagtattgat
     3361 gatcccatct agagctaatg caccaaaagt cccgttatcg accatcaaag aagcattgta
     3421 caataagtta tatgttccga cgatcgataa ctgatctatg ctgaaaccta gccattcagc
     3481 tagttgactg tccactgacg tttgccggga aatgatcaaa ggaacagttt tcagttcctg
     3541 cggatggatg actgtttgct ttgctaaagg atgattccga tgtatcaaaa gtccccaacg
     3601 atcttgggct ggcagtcgaa atgactggta tttttgtttg tttgtcggtt cgattacgag
     3661 tccaaaatca agtaatccgc tatccagttt gtctatgata tcatctgcat tcccgctata
     3721 taaatgaaac tggatatcag gatgcgcctt gatcaacgaa tgaaggctat cagaaatgaa
     3781 agagattgct ttagtttcgc ctccgccaat gtagatttct ccagagatga ttgcgtcatt
     3841 tgttaaattg ttcgccgttt tttctaccaa cgacaagatt tcttttcctt ttgaataaag
     3901 atatcgacca gaatccgtca actgtatctc tcgggagcct cgagtgaaaa gtgtcacacc
     3961 aagttcttct tctaaatctt tcagttgttt tgagagagta ggttgcgtga tatgcagaga
     4021 ttttgctgct cggctgatat ttttttctct agctactgct aaaaaatacg tcagtatacg
     4081 aatatccatt ttttccctcc tgtttttaaa aaagcatacc actaagtata gttaaaagga
     4141 atagttaagt atgttttata agtattagac atcataagcc gtccgttata aagtaaggat
     4201 aaaaggaggg agcaaaatga caagcctcat cgaaaaacaa atcgtcaata aagagattcg
     4261 agtaacggat ccgctctttg aagtgatcca tcagatccaa gcggaaaacg aagagccttt
     4321 agcagcgctg aatactgggt accatgaacc agcagatatc cgcaaacggc tagaaaatat
     4381 cattagtgac aaagtagatg atacggtgac tgttctgctg cctttttata cagatttcgg
     4441 caaacatatt agtataggga aaaatgtctt tatcaatcgt caagtgatgt tcgttgactt
     4501 aggtggcatc tgtttagaag attcagtatt gattggtcct cgtgtcaacc tcatcacggt
     4561 gaaccatcta accgatccaa aagaacgaag aggcttgtca gtaaagccaa tccacatcaa
     4621 gaaaaatgct tggatcggag caggagcaac tattctgcca ggcgtaacga ttggcgagaa
     4681 tgccattgtt gcagcgaatg caactgttac gaaagatgtc ccagacaata ccatcgttgc
     4741 aggaatccct gccaaaatcg tcaaacctgt cgaacggatt atttgaacag taagatgaaa
     4801 taagagatag tgagtataag caagagattt ttcaaaacag gaggagaata gaatgaatga
     4861 tttatcagaa aaagtgattg tcatcatggg cgcttcaagc ggaatcggtg aagcaacaac
     4921 gaagctatta gcagaaaaag gggctaaact agttatagct gcacgccgag aagatcgcct
     4981 aaaagcaata aaagaatctt taccagaagc tgagctatat attcaaacag cagatgtaag
     5041 agattttgca caagtccaag cagttatcga tttagcgatg gaaaagttcg gtcgaatcga
     5101 tgtattgtac aataatgcag gaatcatgcc aaccgctcca ttagttgaag gtcaccgtga
     5161 tgaatggcaa aatatgcttg atatcaacat catgggtgtg ttaaatggaa tttccgccgt
     5221 acttccaatc atggaaaaac aaaaatctgg gcatatcatt tcaactgatt ctgtggcggg
     5281 acatgttgtt tatcctgatt ccgccgttta ttgcggaaca aaatttgctg tccgtgcaat
     5341 catggaagga ttacgtcaag agcaacggca aaataacatc aaatctacga tcatctctcc
     5401 aggagctgtt caaactgaat tatatcaaac catcagcgac aaaaaggctg ctcttgaact
     5461 tcatgaagcg caaaaagaat ggggattaac atctgaagat atagcttctg ctgtagcatt
     5521 tgcgatagaa acgccagacc ggatgtctgt cagcaacatg attatccgtc cgacgacaca
     5581 ggaagtataa tccataacgg ataatatcca gagagaatta cttgttaaat agttattcaa
     5641 acgaccatca atttaaggcg tgaatggaga agaatattct tcatttacgt ctttttatgc
     5701 agaaaaagca aatttattaa aaagaaacaa atgttattta aatatggaca ttgacttgct
     5761 tttacttttt tagttacact gttatttagg aaaagtttca taattcaaag gagaaaaaag
     5821 atgaaagcaa agaatttatt atttagcgtt attcttttag gaaatctagc agttgccaac
     5881 acaacagtgc tagctgaaga atcgacagaa actgtcgcaa ctcagacatc gagttcttca
     5941 gaaacaggaa ttgaaaacga tacagaaagt actttgcagt cagctactgt ctcagaaaat
     6001 acaatttcaa ccgaagaacc aacaactgaa acaacagaac aaacacaaac cgaagacagt
     6061 acatcagcag aagctcagaa caatccagag aatgcagaag aaatcacgaa ccaattgaca
     6121 gatgggcaaa cattgacaca tacccatcaa gcagatttgt ctgaattcga aaactatact
     6181 ggctatgctt tttatgctcg ctcatctgct aattctcaac aagcgttcat tgatagtatt
     6241 gcctcaactg cacaaagttt ggcaagtgcc aatgacttat atgcatcagt catgattgct
     6301 caagccatcg ttgaaagtgg ctgggggaac agtactttgg cttctgcccc taattacaat
     6361 ctatttggga ttaaaggaag ctataatggc caatctgtta cgatgcctac ttcggaatat
     6421 gtgaatgggc aatggattac ggtgaatgcc gcttttagaa aatatccttc gtacaaagaa
     6481 tcattgcaag ataacgtaac ggtattgaag acgacttctt ttcagccagg cgtttactac
     6541 tattccggtg catggaaaag caatacgaac tcttacaaag atgctacggc ttggctaaca
     6601 ggtcgttatg ccactgcgcc taattatggt tctacattga acaatgtaat cgaaacctat
     6661 aatctgacac agtatgatac agcaccaact tcaacttctc cgatgtatcg tctgtataac
     6721 cgtcatacag gagaacattt atatacattg aatgccggag aaaaaaatta tctgccaact
     6781 gtaggctggg aatatgaagg gattgcttgg caagcaccaa acagcggaca acctgtctac
     6841 cgtttataca atccttacag tggagaccat cattacacaa tggcacaatc tgagatcaac
     6901 tctttaacaa aaatcggttg gcgttatgaa ggtctaagtt tttattctgg tggttccaag
     6961 ccaatttatc gactatttaa tccaaatgaa aaaactggta cccatcacta taccttgagt
     7021 gctaaggaaa gagattggct aacaccgatg ggctggagat atgaaggaat aggattttat
     7081 ggttattaaa aagaggtagt gaaaaagctg ccctgcatca agtagtaaga acagccaaac
     7141 aaaaacagat tttcatgttt tttgtttggt tgaacttatt accgcagatg caagtttttg
     7201 aacaccgttt actcaaaaaa ggttgtgaca acatcttgtc ataacttttt tattactgtt
     7261 taagcatact gatccaattt ctaatattcg acgggattct tgcaaaaggc gatttcggaa
     7321 atttttggcg gtgtaaaatt atctactgca gaagagctat cttctatagt ttgataagaa
     7381 tcaataatct tggttttagc gttgtgcttt ctttatcatt ttttccgttc attctacaaa
     7441 aaatttcttt tctctatact tttctattga gagcgttttt tttattacac agctgattct
     7501 ttgacttttt tcttttttta ttctattatt gacaagtcaa tcgttgacga gtcaattatt
     7561 agaaaggagg aaattatgtc tttagaagag attagtcacc ttttatatca aatcaaatta
     7621 gccgatcaaa aaatcgtcca gatctttgaa gaaaatatcg gtataagtct tactagatac
     7681 gagatcatga tgagactgaa agaagaaaat ccattgctac aaagcagttt gcaagaaaag
     7741 ttgcagattg atcaaggagc agttactcgt catctgaagg tattggaagc aaatggctat
     7801 gtttcgcgtc agagaaatcc tcagaacaac cgggaagtgt atgtagcgct aacagaaaaa
     7861 gcccagaaga aaatcaagca ttgtgaaatg gaccataaag atacaggttc cgtgctatcc
     7921 ccggctttca gcgatgtaga aatgaaacaa tttctaacac ttttagataa attgaatacc
     7981 caactttgca atataacaac agaaacgaaa gaggttaata ataatggaac aaaaaaatga
     8041 tttttcaaca attatgactg gtagaagatc cgttcgcgta tatgatgaaa catacaaaat
     8101 cccacacgaa gaaatgctgg aaatgatcaa agaagcgacg actgcacctt cttctgtcaa
     8161 tatgaaacca tggcgctttg tggtcgctga aagcgaggaa gcaaaagaaa tcttgcgtcc
     8221 tttgatccgt ttcaatacga gacaaaatga tacttcttct gcaatggtca tgatttttgg
     8281 cgatatgcaa tgttacgaat atggcgaaga aatctacaac caagcttacg aatcagggaa
     8341 gatgccaaaa gaagtcagag atcaacaatt agctgcgatc attccttatt acaagagttt
     8401 ttcaagagaa gaaatgaacg atgtagtcaa agtcgattct agcttagcgg ctatgcaatt
     8461 catgttagtt gcgcgtgatc atggctatga aacaaatcca attggtggat tcgaagcaga
     8521 tcagttggca gaggcatttg gtttggataa agaccgttat gttccagtta ttattttatc
     8581 aataggaaaa gcggtagaag aaggttacga gtctgttcgt ttagcaccag aaaagatcac
     8641 tatgttcgaa taaaaggttt tcagttacta ttcagtagct attgcttttt ttgaatcgtg
     8701 tgtggtaacc taataaaaaa ggaggtcgga aaaatgactg catcgatgaa attacgtttc
     8761 gaacttttga ataacaacta aataaattca aaagacatgt ataaacaaca atttcatgga
     8821 tggagtatgt ccattttttc gaacgacagt agaataggtg aaagctatga cagatttggt
     8881 gatttagtca tagttatttc gttcagagta ggaaaagatg tgatgcagtt tttttgcaaa
     8941 cagatttttc tgctcttttt tagtatcgat aattgttgtt ttacatgaaa taggagtgta
     9001 aaacatatgg aaaatttagc agtaaatata acaaatctac aagtcagctt tggcaaccag
     9061 ctagaactat ctattgattc tcttcgtgtc tatcagcaag atcgtatagg gatcatagga
     9121 gaaaatggtg tcggtaaatc gactttgctc aaactaatag ccggtgaact ttttcccgat
     9181 catgggaaaa tccaaacaga gatcaccttc aactacctgc ctcaattaac ctatctcgct
     9241 gaagcaaagg acctaaattt ggaattagcc agtcatttcc agttaagact ggaagaaact
     9301 tcagagcgga aatggagcgg aggggaagaa cgaaagatcg agttgatacg tcttctttct
     9361 tcttatgaac aagggatgct tctagacgag ccaacaaccc atctagatag aaaaagtatt
     9421 gatcgactga ttgaagagct tcgttattat tatggtacgc tggtttttgt tagtcatgat
     9481 cgctattttc tagatgagct agcatcgaaa atctgggaag taaaagacgg agaaatccga
     9541 gagttttcgg ggaattatag tgcctatctc actcaaaagg aattggagaa aaagactcag
     9601 ctacgagaag cagagtcgat catgaaagag aaaaaacgat tggaaaaatc gatccaagaa
     9661 aagaaaaaac aagcggaaaa gttggaaaaa gtgtccagta aaaagaaaaa gcaacaaatc
     9721 agactggatc ggttgtcttc ctctaaacaa aaagacagtg tacaaaaagc catccaaaag
     9781 aatgcgaaaa cattagagag aagactccaa aaaataggag aaacaaccaa gccgcagcag
     9841 atgaaacaaa tccgttttcc agtaccaaaa tctcttgaac tccacagccg ctatcccatc
     9901 atgggacaaa atgtccaatt ggaaagaagt gggagaacat tactggtaaa tggtgatttt
     9961 cagttttctt taggtaaaaa aatcgcgatt gtcggtgaaa atggttcagg taagacaacc
    10021 ttattagaac atatccgcaa acaaggagaa ggaatccttc tctctccgaa agtaagcttt
    10081 caagtatatc agcaaaaggg ttatcaaatg acatctgaag aatccatcat tcgttttgtc
    10141 atgagacaaa cagagttttc agaatcgctt gtccgtagtt tgctgaatca cttagggttt
    10201 gctcaggaaa ctttgacgaa accgttatgt acattaagtg ggggagaagc gacccgtctg
    10261 acgattgctt tgctttttac taagccaagt aatgtgttgc tgttagatga accgactaat
    10321 tttattgata tggcaacgat cgaagcttta gagaagctaa tgcaaatata tccgggaacg
    10381 attttgttta cttctcatga ttcttacttt gtcgagcgta cggctgatga agtttatgaa
    10441 ataaaagggc agaaaataaa aaaagtactt acgagaaatt tttaaatgtg ctacgatgat
    10501 tttcgtgaca aatttaaagg agtggtacag attgcctaca aataaaaaag aaggaataat
    10561 ttttacaaca atgatgtgtt ttttgatggt cttggggatg agcgcgtata atttatggct
    10621 gcatcaagat cttcatttat caagtttgtt gattggtcta gtgcctggat tcgtggtggc
    10681 tttcattttg gatgtcttca tcatcggtgt gctggcgaaa aaaattgcct ttaaactacc
    10741 agtaaatcca gaaagcaagg tccaaatgat cttaactatt tcatgcttga tggttttagg
    10801 aatggtgaca tgtatgtctt tattcggtgt gatcatggaa caaggaatac ctgatcaatt
    10861 attctctgct tatatgaatg cttggaaaat gaatgtgatt gccgctttac cgttgcagtt
    10921 gatcatcgtt ggcccgcttt caagaagtgt gctaaaagca attcaaagta atgaatgatg
    10981 gaacactgtt atttgaaaaa aggccttggc atgctttttg tcatagtctc ttgaatgcag
    11041 ttctagaaaa atagtgatcc gaacgatagg ttttggcctg tcgttcggat cgctttttct
    11101 tttccaactg tattgctctt ttattgaaga taatttgctc ttcaatagat atttctagaa
    11161 aggttctgtt tatcccttac tcaaaaaact gctgcatttg ttctcttact tgctgacacc
    11221 attggagttc ctgctgacaa agcataattc tgcgctggat cacaacataa cgtccaaatt
    11281 cttgtttctt gtctagcgag aggtcattca attttttttg atttttttgc atgattttgt
    11341 tcagctgttg ttctctagtg aatagcagtc cagtagctgt tgttttatcg agcgtcgaaa
    11401 aagcatagat ttttgctaga aattcatctt tttgcgtagg agcgggagtt tcctctttga
    11461 tccattcctc tacaagagca cgtccttcat cagtcaagtg gtagatcttt ttttgagagt
    11521 gctcgtcttt gtccaaaacg gtcacataac cctgctggtc taacttggca agcgttgtat
    11581 agatctgact gtgatgggct tcccaaaaaa ttgtgatcag ctgcttcaat tcatacccag
    11641 aacgtggttt ggataaaagc acgcataata atatataggc taaagtattc ataaaaatcc
    11701 tacctctcgt cttattttct ttattatatc acagtaaagt ttccacgttt tacatgtgca
    11761 tattgacatg taaaataaat catgtatgat gctaatagag agaaagggat ggaaatattg
    11821 gagaatcaaa tagatataca agaacaaaca aatgaagaaa caaaggggaa aagttcagcg
    11881 ctagttgtag cagctacctg tatgatcgct tttatgggag tgggtctagt agacccaatc
    11941 ttaaaaacga ttgctgcaca gttaaacgct acgccagctg aaacgaccct gttatttact
    12001 agttacatgt tagtcactgg tgtggtgatg ctatttacag gtttcttatc tagtcggatc
    12061 ggtttgaaaa aaacattgat gacaggattg tttatcatcg ttgtattcgc tggtcttggc
    12121 ggattatctg ggaatatcgg aatgctgatt gggttacgtg ccggctgggg tgtcgggaat
    12181 gctttatttg tctctactgc tttagcaacg atcgtcagta ttttagttgg taacacagaa
    12241 aaagcaatca tgatgtatga agcagcactt ggctgcggaa tggctgttgg ccctctagtc
    12301 ggtggggtct tgggcagtgg ttcatggcgt tatccatttt ttggggtagc atttttgatg
    12361 ttttgcggtt ttatagccct agcggtactt ttacctgaaa ctgagaaacc aaagagaaaa
    12421 gtaggccttt gggaaggtgt caaagcgtta agcaatcata aacttcgttc agttggacta
    12481 atcgcattgt tatataattt cggattcttt actttacttg cgtatgcgcc atttttattg
    12541 attggctatt ctgaattaga agtcggtttt gtattttttg gctggggtgt gcttctggct
    12601 attgcttcga ttttcgttgc accggctctt gagcggaagt ttacgactta tcggacaatt
    12661 ttagcagcat ttgtcttatt cattatttgt ttgctgttat taggagctgg aacttctatg
    12721 ccagtccttg taccgatcag cattgtgtta gcaggatttt tccaaggaat tatcaataca
    12781 ctgttgacga cgattgctat ggaaatacca ggtctgcaac gaaacgttgc ttcgtccagc
    12841 tatagttttg tccgcttttt cggtggagct ttagcaccat ttattgcagg tgtgattggt
    12901 gaacatattg gtgaaagcta tgcgttttat tttgcaggtg ccattgttct tatcagtgta
    12961 tgttgtatca tttatcaccg taattatttt gctacaaatg aagaagaata agaaatcaga
    13021 ggttgtgaaa aagctgtcct gcatcaagtc ataagaa
//
LOCUS       DACDEG010000274        12476 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.guided.072, whole genome shotgun sequence.
ACCESSION   DACDEG010000274 DACDEG010000000
VERSION     DACDEG010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.guided.072"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            55..1176
                     /locus_tag="IV142_002968"
     CDS             55..1176
                     /locus_tag="IV142_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8486524.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            1434..2936
                     /locus_tag="IV142_002969"
     CDS             1434..2936
                     /locus_tag="IV142_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP8486525.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            3003..3530
                     /locus_tag="IV142_002970"
     CDS             3003..3530
                     /locus_tag="IV142_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8486526.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            3741..4802
                     /locus_tag="IV142_002971"
     CDS             3741..4802
                     /locus_tag="IV142_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP8486527.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            4805..5701
                     /gene="murQ"
                     /locus_tag="IV142_002972"
     CDS             4805..5701
                     /gene="murQ"
                     /locus_tag="IV142_002972"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP8486528.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            5715..7697
                     /locus_tag="IV142_002973"
     CDS             5715..7697
                     /locus_tag="IV142_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8486529.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(7762..8208)
                     /gene="nrdI"
                     /locus_tag="IV142_002974"
     CDS             complement(7762..8208)
                     /gene="nrdI"
                     /locus_tag="IV142_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP8486530.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            8419..9843
                     /locus_tag="IV142_002975"
     CDS             8419..9843
                     /locus_tag="IV142_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8486531.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(10024..10797)
                     /locus_tag="IV142_002976"
     CDS             complement(10024..10797)
                     /locus_tag="IV142_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8486532.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(10794..11699)
                     /locus_tag="IV142_002977"
     CDS             complement(10794..11699)
                     /locus_tag="IV142_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8486533.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(11853..12173)
                     /locus_tag="IV142_002978"
     CDS             complement(11853..12173)
                     /locus_tag="IV142_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP8486534.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         4017 a         2208 c         2689 g         3562 t
ORIGIN      
        1 gtttgaaaaa aatgaaaaaa agtgtcttac ttaagaagaa agtgagggat acagatggat
       61 attttggcgg caattgattc atttaaagga agtgcttctt catttgaact aaatcaggca
      121 gttctaaatg gctgggaaga tcgtaaagga agaaaaatca atgtgccgat tgcagatggc
      181 ggagaaaata ctgcagaagc aatctattac gcgctcaggg gaacatggcg taccattgat
      241 gggatggatc tcttattccg tcagcgaaaa atcagctatt tattgacaac ttggcaagga
      301 aaaaacaccg cagtcatcga atctgcacaa gcattggggt tagatctact tgataaacca
      361 acggatcaaa ctatccgact tgcttccagt tatgccttag gagaacttat taaagatgca
      421 ctgcttcaag aagtagaaca aattattgtc actttaggag gaagcgggtg tagcgatggt
      481 ggtctaggat tactccaatc cttgggtgct tctttagaag ggtacacaga aggaaatcct
      541 ttattatcta ctaaaaaact tcattttgat cgaagaaacg ttcttgtttc taaagtaaaa
      601 ttgattatcg ctgcagatgt taccaatcca tatacaggtc aacaaggagc tgcttggcaa
      661 tttggaagac aaaaaggagg aatagatacg acgctagctt tcttagataa acaagcagga
      721 aagattgctc agcaaataaa agatttctgg gaggtggatt tagatgatat tccaggaagt
      781 ggagcagcag gcggtattgg tggagccctt tttttgttgg gagcgaagat ggtttctggt
      841 ttcgatttag tcagccaaat cactggattg gaaaaaaaga tcgaacaagc agatgtcatc
      901 attacaggag aagggcggat cgatcagcaa acgatccacg gaaaagttcc atacggtgta
      961 gcagtgcttg caaaaaaata tcgaaaacca ataatcgcta tttgcggaag tagagaccaa
     1021 gagctagaag gactttctga ttttctccca attgtcttaa gtattcattt gggtccaata
     1081 ggtttagaag aagcaatgga acacaggcaa acattagaaa aaacaaaaat cttaggacag
     1141 acacttagca gatttttcac catcctcccg aagtagttga caaaatcatt ttctttatgc
     1201 tatcgtaatt tcaaatatag gacaaagtga gggatccgtg cattgaaaaa ttaagtcgat
     1261 tgatgaaagc tgcattcaga ctgtaccttg tggagacaag gaagcagtgt gcagattttc
     1321 gggattggct tggttttttc gatgtatggg tctttttgtg tgtatccaac ccttgcatcc
     1381 tctttagcca gttctgaaaa taggactggt ctttttttac gaggtgatgg aaaatgtcta
     1441 aaatcgaaat aaaaaatctg acattcggct acgacagcca aggcacatta ttatttgaac
     1501 aagcaaatct aaattttgac acacaatgga aactaggact tatcggacga aacggtcgag
     1561 gaaagacaac tttactgaat attctacaaa acaaactacc ttatcaaggg caagtaatcc
     1621 atcagcaaga atttgcctat ttcccgcaac agacaaaaga taaagaacgt ttaacctatt
     1681 acgtgttaaa tgatattacg gattttgaga tatgggaaat cgaaagagag ctccaattga
     1741 tgcaaacaga tcctgaaatc ttatggagag aattcagcac actatcgggg ggagagaaga
     1801 caaaagtcct actggcactt ttatttgtgg atgacactca tttcccgtta atcgatgaac
     1861 caacgaatca tttggatatc tctggtagaa aacaagtagc ggcttatttg aaaaagaaaa
     1921 aacaaggctt catcgtggtc agccatgacc ggggatttat cgatgaagta gtggaccatg
     1981 ttttagcaat cgaaaaaagt caactggaac tttatcaagg gaatttctct atctatgaag
     2041 aacagaaaaa acttcgtgat gaatttgaaa tggctcaaaa tgaaaaattg aaaaaagaag
     2101 tcagtaggct aaagaaaaca gcagctgaaa aagccgaatg gtctcgttcc cgagaaggag
     2161 ataaaacaaa gaaacaagtc ggattcatcg atactgaatc tagacgagtg aataaaggag
     2221 cagtgggtgc tgatgctgca cggacgatga aacgatccaa agcaatcgtg aatcggatgg
     2281 agacccagat cagcgagaaa gaaaaactat taaaagatat cgaatatatc gattctttga
     2341 cgatgaatag ccaagcgtct caccataagc gacttttaag cgtagaagat cttcaattag
     2401 ggtatgaaaa tctgttattc gagccaattc attttacaat cgagcctcat cagcgggtgg
     2461 cgatttcagg tcctaacggt gcaggaaagt catccattat ccattatctt ctgggggcat
     2521 tcaacggcaa ggttatagga gaaaaatacc agccaaaaca tctgagcatt agttatgcaa
     2581 gccaaaatta tgaagacaat cgaggaacgt tggcggaatt tgcagagaaa aaccaagtag
     2641 actaccaagc atttttgaac aacctccgaa agcttgggat ggaaagagat gtttttcata
     2701 acaagatcga gcagatgagt atgggccaac ggaaaaaagt ggaattggct aaatctttat
     2761 cacagccagc tgaactatat acatgggatg aaccattgaa ttatttggat gtcttcaatc
     2821 aagaacaatt agaacaactg atcttgaacg tgaaacctgc catgttacta gtggaacatg
     2881 atcaaacctt cctggataaa gtatctactg agattatttc tcttgagaga atctaaccct
     2941 tgtaatctca agaacaacaa ggtatggtta aggggaactt tgccaacgaa ggagagaatc
     3001 acatgcttta tgtaacgaga cacggagaaa caacttggaa cgcacaagga ttagtttgcg
     3061 gacgggcaga tgtgccgctg actgaaaaag gacagatgca ggcacagaaa ctagcagaaa
     3121 aggtagtaga tttgcctgtt ccaatcacga aaatcatcca ttcccctctt caacgagcaa
     3181 gagatacagc acaagcagtt gctgatcgtc tcagcttgcc gctgaccgtt gatgaacgat
     3241 tggtagaaat ggattttggg gattatgatg gactgccaag caaagatgag aactttcaaa
     3301 aagcacgttt agcgttcgct gtacgattcc caaatgggga gtcagtctta gatgtgtatg
     3361 cacggatcgt tcctttattg aaagaatgca tcgaagacga agaaaatgtc tatctgttag
     3421 tttgccataa tgccttgata agagtgatca atgcttattt tcatccgatg cctaatgagg
     3481 ggttctttac attcatggta gataatacag aattggtttc ttatgagtaa ttatgttttt
     3541 gtgttattta aataaaatat ttcaaggcac tgcttttgct tagcagtgcc ttttttaggt
     3601 tatgacaatt cttttgataa tataaactca acctttagaa atgaaattta attattttaa
     3661 taaaagtatt gaaattttgt ttctttagat atatactcta gtcatagaaa tgaagacgga
     3721 tacagaaaaa gagggataat atgtttggct tttcaatatt tatgaacgat tctttgtcag
     3781 aagagaagaa acagtatatc cgccaaatgg catccaatgg ctttgtcggt attttcactt
     3841 ccatgcatat accagaagat gatgtttcgg catatcaaaa gcgattgatc gaactaggaa
     3901 gcgaagcgaa aaagaataaa ctagaattga tggtcgatat ttcaggagag gcattggaac
     3961 gagcgggttt ttcattagaa gatctaacgc ctttgaaagc aattggtgta acaggtttaa
     4021 gaatggatta tcatatttcc aatcagcaga ttgccgactg gtcacatcaa ttgaaaatca
     4081 gtttgaatgc tagcacgatc acgcctaaag atatcgatga attgaaagaa gcagaagctg
     4141 atttctcaca gattgaagct tggcataatt attatccacg tcccgaaaca gggctggaca
     4201 aagaatggta tcagaaaaaa aatcaatggc tcaaaattca aggtctcctt gtccaaggtt
     4261 ttgtaccagg agatacagag ttgagaggcc cactatatca aggattgcca actttggaag
     4321 aacatcgagg gatccatcct cttgcaggcg ctctagatct attagcaagt aacacggata
     4381 ttgtttacat cggagatgca ggtctgtcag aaaacgttca ggagcaattt gcttctttcc
     4441 aaaaagaaca gacagtcttg ctccatacgg aacctgtaga tgaagaattt tacgaatata
     4501 tattagggaa gcatacgaat cggcaagacg atgcaagaga tgtcatacga agcgtagatg
     4561 cacgttttcg agagattccg cctatcccag caagaaatac agctacgcgt atgaaagggt
     4621 ctattacttt agataacgaa aagtatttgc gctatatggg tgagatccaa ttaaccaaat
     4681 atgatttgcc agcggatgaa aaagtgaatg tagtagctaa agttatcaaa gaagagctac
     4741 cattgatcaa ccaaatcaaa gctggtatga actatcaatt tataagaaaa gaaggaagat
     4801 aaaaatgagc aaaatcaatt tagagaacct aacaacggaa cgcagaaacc aagaaacttt
     4861 tggattagat gagatgagta tttctcaagc cgttcaacta atgaacaaag aagatcagaa
     4921 agtggccgct gcggttgaaa aagaactgac aagtatcgaa ccagtgattg ctaacacgat
     4981 tgagtccttt aaaaaaggtg gacgtctgat ttatatggga gccggaacaa gtggaagact
     5041 aggtgtgtta gatgctgctg aatgcgtacc gacattcggt gtagaaccgg aaatggtcat
     5101 aggtctaatt gctggtggag aagaagcaat gactgtagct gtcgaagggg ccgaagattc
     5161 ttatgagcta gggcaagaag atctaatcaa gctgaatttg acaaaaaatg atatagttat
     5221 tggtatcgca gctagcggtc gtacaccata tgtgatcggt ggattagatt acgcaaaatc
     5281 gatcggtgct gtcacaggaa cgatttcttg caataaacaa gccgaaatca gtaaacacgc
     5341 ggatttccca atcgaagtcg atgcaggtcc agaatttttg acaggctcta ctcgattgaa
     5401 gtcaggaaca gcacaaaaac ttatcttgaa tatgatttct actatcagta tgattggtat
     5461 aggtaaagta ttcaataact tgatggtcga tgtcaaacca acaaatgaaa aactaatcga
     5521 acgctcaaaa cgaatcatca tgcaagcgac agaagctgat tacgaaacag ctgaaaagta
     5581 ctttgaagaa gcagatcaaa atgttaagct ggctatcgtc atgattttga caaatagtga
     5641 taaagaggaa gctcaagcaa aattagctga agcaaacgga tttgtaaaag ggaccttatg
     5701 agggggaaaa gaccatggca aaaaacgaac tgactttgga agaactggca aaacaaattt
     5761 ataaaggggc aggcggcatg ggcaatgtag aaagcattgt ccactgcatg actcgtgtgc
     5821 gtatgaagat catcaatgat gatcttgtaa atgtctctga attaaaagcg attccaggag
     5881 tgcttggtgt agtagaagat gaacagcttc aagtcatcat cggacctggg aaagtgaaca
     5941 aagtagcaca gcaaatggtt gatttggcag gcgtgaaatt aggagaaaaa ctttcttctg
     6001 taagtacagc tacagcaaat tcatcagcat ctggaaaaga ccgtgtaaac gaacgggcac
     6061 aagaaatgaa agcggcacaa aaatcgaaac aaaaaccatc taaaatcaaa tcggtattaa
     6121 aagacatttc taatatcttt gtgccaatga ttcctgcatt tgtcggtact gggatcgtgg
     6181 cagggattgc cgcagtttta gcaaacttag tcacagccgg aacacttgat gcagcaacat
     6241 ggcaacaata tatcgatgtg atgaacatct tgaaaaacgg gatgtttagc tacctagtta
     6301 tctatacagg tatcaatgct gcacaagttt ttggtgcaac accaactctt ggtggagtta
     6361 tcggggccgt tgtgatgctg acagggatga atccggaagc accactgaaa aacttattta
     6421 ctggtgaagc tcttgcagct ggacaaggtg ggatcctagg tgtcatcttc gctgtttggc
     6481 tattgtcaat cgttgagaaa aaactgcata aagtagtacc tgatgcggta gatatcatcg
     6541 ttacaccaac tctttcattg attgccatcg gtctgattga aattttcttc atcatgcctt
     6601 tagcaggttt catttctgat ggcatggtcg gtgggatcaa ctgggtactt ggcgttggtg
     6661 gagcatttgc cggcttcgtt ctaggcacat tattcttgcc gatggttatg ttcggactgc
     6721 atcaaatttt gactccaatc catgtgcaaa tgattgatga aactggtcgt acgctattgc
     6781 ttccaattct tgcgatggct ggtgcaggac aagtgggtgc agcattggca ttatggattc
     6841 gctgcaaaaa agataaagaa ttaacagaaa tgatcaaagg agctttacca gtcgggatcc
     6901 taggaatcgg cgaaccattg atttacggag taacattacc tctaggacgc ccatttatta
     6961 ctgcttgtat cggtggaggt atcggcgggg cagtgatcgg aatgatcgga aatgtcgggg
     7021 ctatcgctat tggaccttca ggagccgctt tgatcccatt gatctctgac ggtaaatggt
     7081 taggctacat ccttggatta ttggcagctt atgctggtgg cttcgttgct acattcttct
     7141 tcggtattcc taaagaccgt ttagaaaaag aagaattagc gactgaaaca gccacagtaa
     7201 atgaagtgcc tgaaaaagta gtacctcaaa cttcagctga aagtgtgact atcctctcat
     7261 cagttgctga cggaacagtt gtttctttag ataaagcaag tgatccagtt tttgcacaaa
     7321 agatgatggg tgaaggctac tttgtagaac cagaaaacgg acatatctat tctccagtta
     7381 ctggggtagt cagctctgtc ttcccaacga aacacgctat tgggatcaca acgccaaatg
     7441 gtttagaaat cttgcttcat atgggaatca acacagttga cttaggcggt acgccatttg
     7501 atgtgaaagt aacagaaggg cagcaagtga cacctgatac gcttgtagca gaagctgact
     7561 tagaagcgat caaagcagca ggcaaagaaa catccatgat cgtcttggta accaatatgg
     7621 atcgtgtaaa aaactttgtc ttggaaaaaa caggtaaagc aaaagcaaaa gcacctgtga
     7681 tggatgtcaa agcttaatat gatagaaaca atagaaacga aagaagaaac tgaaatctat
     7741 ttttcagctt cttctttttt attatttatt gttttctgct aaaatctgat aaattcgtgt
     7801 cacatcagat ggtgttcccc gtaattcata atctgccaag aaaggaaagc caaatgtttg
     7861 tgcatattgt ttggctgtta gacaatactg attattgaag tttttatttc cgctaccgac
     7921 tacaccaaga caaagtttat gattgtcttc atgttccaga tattcacgca tcgtttcggt
     7981 caagatttct gtatcaccgt tatctaaacc atttccgccg tctaaataag taggaacgaa
     8041 agtaaaaaat ggttctgttt ctttcgtaaa atcactattt tcatggattt cttttaaagt
     8101 aacttcagga ttgacaggat cttctgcatg cattttttca gcatattctg ctagatgttt
     8161 ggcaaaggca cgcgtatttc ctgaaatcga tatatataaa atgttcatgg atcatcctcc
     8221 aattctttcc ttttagtata gcatcttttt taagtttttt ggtggtgtaa tttggaaagg
     8281 gactaggcgc tttgcttgta taatgagaaa aaagccggta gaattatatt tctattaaag
     8341 tttttattaa ttaagtaaaa ataaaaaagg tttgttatac tttttgataa aagcggtttt
     8401 attttggagg atgcttacat ggatacagag caacagaaaa aaggaattgg tttaattcca
     8461 cttgctgcct tagtaattgg ttcagcgatt ggaggtggcg tatttggtat tatgacggac
     8521 atctccggat cggctggcgg cccagctatt ttcagttggg tactcgtagg aacagcaatg
     8581 ttgatgctct ccttgtcgat caataatatc aataaaaaac gtccagattt aaaaggcggg
     8641 atctttagtt acgcagaaga aggttttggc cgtttcgctg gttttatcag cggttggggt
     8701 tactggctca catgctggtt aggcaacgta gcttttgcaa cattgttgat gagcgcatta
     8761 ggatatttct tcccagtatt tgaaggtggg caaaatctgg catcgatcgt gatgagtaca
     8821 gtctttttat ggggatatgc atggctagtg aatcggggag tcgaaaatgc tagtttcgtc
     8881 aatgctatcg tcacattctg taaattagtg ccattatttt tatttattgt agtagctatc
     8941 agcacgtttt cgatccaaca attcattgat aacttttcta gcgacattat attgaatgcg
     9001 aatggacagc cgatcccgtt ttccagtcaa gtattgagtt gcatcatggt gatgctttgg
     9061 gtatttgttg gtattgaagg agcctctgtc gtaggttcac gagcaaaaaa acaatcagat
     9121 gttgggaaag caacgattct agggatattc ggcttgatta ttatttacgt cttggtttcc
     9181 atgcttccat atgggacaat gacgatgaat cagctgcaaa cgatcaaggc gcagcctgct
     9241 atgggctatg tgtttgaaat gatggttggt aaatggggag cagtcgtgat caacggcggt
     9301 ttgatcattt ctttagttgg tgtttggctc tcatggacga tcttaccgat tgaaacaatg
     9361 ggaaacatgg ctgaagacgg acttttgcct aaagcatggg gaaaactcaa caaaaatggg
     9421 gcaccaactt atgctatcgt attgacgacg ttatgtacga atgtcttttt actttcatta
     9481 ttattcactg acagtgctta taactttgct tacacacttg gtacagcagc tattttcttc
     9541 acgtggcttt tcttaggatt gtaccaaatg aaactttctt atcagcggaa agaatgggtc
     9601 caatttttag tagggctttt agcaagtgcc tttcaagtat gggctatgct agtcgtagcc
     9661 tttaatgaag taatgcttgt tcttgttctt ttcttgccag gcatttattt ttacctcaaa
     9721 gcacgtaagg agcaaaacga gcctgtacaa catatccact tagataagat tttacttata
     9781 ggtgtgacga tggtcggagt gattgcaatg gttttatttg ccactgggat gcttcacgta
     9841 tagaagtata aaagaagtat aaagagaggt tgtgagcctg tcgtttattc aaaaacatac
     9901 taggaaagga gctgtgacac aaatgttgtc acagctcctt tttcagaata aacggtgttt
     9961 aagagacagc caatcggcaa taaggctgaa cgtctctttc acagcctctt ttttgatgag
    10021 tcatcataca ttttttcgct taaatacgag atatcctaat gctaagaaca aaacgatgta
    10081 agctaaatta ccgaatagca tttgattagt agaaagatga gtcaattgct tcatgatctc
    10141 ttcgtttcca atttggtttt gcagattcaa catactgatc ggattccatt tgatccactc
    10201 ccatttttgg atgagtgcaa taaggatacc agatatgatt gacgaagcaa aataaaagac
    10261 gattcctgct gagatggatg cgccggaact attgataaag caagcaatca tcaacactaa
    10321 acttaaaatc agccataaac cgatataact acccaaagta taagtgaaca agctttggat
    10381 cagtgtttga ccagtagaaa ccttctccgt gaaggaaatc gaagggaaaa gaatcagttt
    10441 catcaacaca gtgacaataa tagctaaaag gtataaataa acagaataaa taactaatgt
    10501 gatccatttg cttactaata tttctcctcg agaatacttt cgataaagga gatttttcaa
    10561 tgtaccatat tgtgcttcca tcgaaatgat cgtgcttgca gctgcaatca tgataaagac
    10621 aatccaagat gtggcactaa aaagctgagc agtcatttca actggatcaa tgatcgtcgt
    10681 gtattttttg gctagaagtg ctgttcctat gagcaaaaca actaatagaa cagataaaac
    10741 agcagttgac tttttcttga tcaatttaaa taattcttgt tttacaagca atgtcatttt
    10801 tcttcttccc cctttactga atccagaatt tccaatagag aagattccaa atcattcgtc
    10861 agatgctgga cgtctgtcac atggatattt tgtgcagaca atagctggat cacggttcct
    10921 aataattctt cgttttcttt taaaagcttg attttatcag gatcttccaa agtatatcca
    10981 gcttctgtca agatcgttcg agcagctaga tcatttgttg tagagataac gatgaaatct
    11041 tgcgctgttt ggttcaattc agccattgta cattgtttga tgatctttcc ttgattgatc
    11101 aggatcagct catcgaccat tttttcaagc tcgctgagta agtgactgga gatcaagaaa
    11161 gaggtgccct gagtagccaa gtcggtaatg atttttcgca catctcttgt tgcttgtgga
    11221 tccaaaccgt tcatcggctc atctaaaata acgatagacg gatggttcag taaggcaaga
    11281 gcaattccca atttttgttt cattcccaac gagtaggatt tcgctttttt gtgaatgtac
    11341 ttttccatct tgagctgatt cacgatagtg tcgatagcag atacatcgtt ggtttcagag
    11401 aataacttca gatgatcata gcctgttaag aatggataga tacccgggta ttcgatcaat
    11461 gctcctactt gttccaaccc tttatgacta gagggagaaa tctcattttc accaatacag
    11521 attgttccat ctgagtaatg gatcagtcct aaaatagctt tcatgatcgt tgtttttcca
    11581 gcgccattag gacctactaa tccaacgatt ttgccagggg ataagctaaa actgacatcg
    11641 ctgagggctt gtttgtgacc aaagttcttg gataattctt ggaccgaaag acttttcaaa
    11701 aatattcact ccttttctat agatagtaca agcatacacc tgatgagtgg agaagtcgct
    11761 aggtcattgg acttatttct tattttttca taaaaaaatt gaatattaaa taaaaaggct
    11821 tattttttct gatcagtact tattccaagt gtctagtggc cgctagtaat cttgatgcca
    11881 atgatactga tgagtagcat ccccacaaag aaccaagtgg aaagattcag cttatcatga
    11941 aacaagacca caccaattat gaccgaacca actgcgccaa tccctgtcca gataggataa
    12001 gagagactca tcggcaaact tttgattgcc tttgacaaaa agtaaaaact agcaatcatg
    12061 ccaatcactg tgtaaaaaga ataattgatt ttagaaaaac catcactcca tttcatcatc
    12121 gttgaccaga atacttccaa aattcctgct atgattaatt ccatccatgc catacttaca
    12181 ttcctcctta actataaaaa aatacacaaa aagaggaaaa tcatcccttc aaagacctaa
    12241 tggaatgatc atcctcttta tcctacccgg tggcagattg tatatctttt tctgtagtat
    12301 agcacaccta tccagatatc ggcaatctca tactttttag taaagtgtac caagcactga
    12361 cggatatatc gttagcggaa aagaagatga atgctttgca atttcataga aagaagttgt
    12421 tatttattaa tcatttaaaa aaaacagtaa ctagacctta tttctatata ttatcg
//
LOCUS       DACDEG010000275         8237 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.guided.106, whole genome shotgun sequence.
ACCESSION   DACDEG010000275 DACDEG010000000
VERSION     DACDEG010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.guided.106"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1742
                     /locus_tag="IV142_002979"
     CDS             <1..1742
                     /locus_tag="IV142_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8486535.1"
                     /translation="IAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQG
                     NSPLIQTVRAYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLG
                     ISELDKKISTLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRG
                     ALLMVTHDRYFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRL
                     YKQELAWMRAGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEI
                     KDGSYRINDQTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGE
                     TVHLAYYTQENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTL
                     IGKLSGGEKRRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSH
                     DRYFLDKVADKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKK
                     KLSYMEQKEWETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEK
                     MARWEYLSEWVED"
     gene            1754..2701
                     /locus_tag="IV142_002980"
     CDS             1754..2701
                     /locus_tag="IV142_002980"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP8486536.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            2721..3248
                     /locus_tag="IV142_002981"
     CDS             2721..3248
                     /locus_tag="IV142_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8486537.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(3311..3964)
                     /locus_tag="IV142_002982"
     CDS             complement(3311..3964)
                     /locus_tag="IV142_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP8486538.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            4096..4938
                     /locus_tag="IV142_002983"
     CDS             4096..4938
                     /locus_tag="IV142_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8486539.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            5096..5992
                     /locus_tag="IV142_002984"
     CDS             5096..5992
                     /locus_tag="IV142_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8486540.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            5995..6708
                     /locus_tag="IV142_002985"
     CDS             5995..6708
                     /locus_tag="IV142_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP8486541.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            6728..7246
                     /gene="msrA"
                     /locus_tag="IV142_002986"
     CDS             6728..7246
                     /gene="msrA"
                     /locus_tag="IV142_002986"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8486542.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            7243..7470
                     /locus_tag="IV142_002987"
     CDS             7243..7470
                     /locus_tag="IV142_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP8486543.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            7622..8173
                     /gene="lepB"
                     /locus_tag="IV142_002988"
     CDS             7622..8173
                     /gene="lepB"
                     /locus_tag="IV142_002988"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8486544.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
BASE COUNT         2948 a         1431 c         1682 g         2176 t
ORIGIN      
        1 ttattgccgg tatcgatagc agcgacggtg atcggcaaac agtgttttat ccaactgatt
       61 accgtattgg ttatctttct caagcagctg aattttccga tgaattaact gttctccaag
      121 cagtctttca aggaaacagc cccttgatcc aaactgttcg agcatatgag gaagcattga
      181 tcgaactagg ggaaaacgga gaagaccctg atgtgcaaaa acgttatgct aaagcagaag
      241 aacaaatgaa taaagaagat gcttggacta cagataccaa cgcaaagatt attttgcaaa
      301 aattaggaat cagtgaatta gataaaaaaa tcagtacttt atcaggtggt cagaaaaaac
      361 gagttagtct ggcgcaagtg ttgattgatg aacccgattt actattacta gacgaaccaa
      421 caaaccattt agattatgaa gcgatcgagt ggttggaaaa ctatttgaaa caataccgtg
      481 gcgcactatt gatggttacc cacgatcgtt attttctaga tagagtagca aaccgaatct
      541 tcgaactttc atttggaaaa ttatacgagt ataaaggaaa ttatgaagct tacgtcttag
      601 aaaaagcaga acgtgatcgt gtagaagtag aacaagaaga aaagcgcaag cggctttata
      661 aacaggaatt agcttggatg agggctggcg tacaggcacg tggcaccaag cagcaagcac
      721 ggatcaaccg tttcgaagat ctaaaggaga acttgtacca agtaaaccaa gaagatgatt
      781 tagagctgaa cctagcgaca caacgtttag ggaaaaaagt actggaaatc aaagatggca
      841 gttaccgtat aaatgaccaa acactgctag aacatttaga tttattgatt caatctcggg
      901 aaagactagg tattactggt aaaaatggtg cgggaaaatc gacattgctt aatattctag
      961 ctggcagaat tcccctggat agcggtacaa tgtctatcgg tgaaacggtt catcttgcgt
     1021 attatacaca agaaaatgaa gaaatggcac cagataaacg gatgattgct tacttacaag
     1081 aagctgctga agaagcaaaa acagcagatg gcagtcaaat cggagtagca gaattacttg
     1141 aacgattttt atttccaaga tttatgcatg ggacactgat aggcaaactt tcaggcggag
     1201 aaaaacgccg gttatttttg ttgaaactgc tgattcagca acctaatgtt ttattattgg
     1261 atgaaccgac caatgatttg gacatagcaa cattgacgat tttggaagat tattttagaa
     1321 gttttccagg tgctgtgatc actgtttccc atgaccgtta tttcttggac aaggtagcag
     1381 ataagttatt agtattccaa ggaaacggaa aacaagaact ttattacgga aacatgtcca
     1441 gttatttact gaagcaaaaa gagacccagc agcctgctga aaaagcgaaa ccaaaaatac
     1501 agagcaagga acctgctgaa aagaagaaat tatcttacat ggaacaaaaa gaatgggaaa
     1561 cgattgaaga tgagattgca gaacttgaag aaaaaatctc attgctccaa gaagagatga
     1621 atcatcaagg agataatttc actcgattac aagaattgca aaacgacgta tctgaaacag
     1681 aagcacaact tgaagaaaaa atggcacggt gggaatactt gagtgaatgg gtagaagatt
     1741 aggaggaaaa aggatggaac aagcgtatct tgacttagga agaaaattgt tggaagaagg
     1801 gcatcgtaaa gaagacagaa caggcacagg cacattaagc ttattcggat accaaatgcg
     1861 gtttgatctg caaaaagggt ttcctttgtt aacaacgaaa cgagtacctt ttggattgat
     1921 caaaagcgaa ttgctttggt ttttaaaagg agatacgaat atccgctatc tattacaaaa
     1981 taaaaaccat atttgggatg aatgggcatt tgaacgctac atcaaaagtc cagactataa
     2041 tggaccagat atgactgatt ttggccatag atgtttgaca gatcctgaat tcaatgaaga
     2101 atataaaaag cagagcaagc ttttttgcga gaagatccta accgatgatt cctttgctga
     2161 aacatacggc gatcttggac acatctacgg ctatcaatgg cgtcactggg aaacaaaaga
     2221 cggtggcttt atcgatcaaa tcaaacaagt gatcgaagca atcaaaaaga ctcctgattc
     2281 tagaagattg atcgtttctg catggaatcc ggaagatgtt cctagcatgg ctttgcctcc
     2341 ttgtcatacg atgtttcaat tttatgttca agagggacgg ctaagctgcc aattgtatca
     2401 acgcagtggg gatgtgtttc taggcgttcc gttcaacatt gcaagttatg cgttgctgac
     2461 tcacctaatt gcacatgaaa caggtttagc ggttggcgaa tttgttcaca cattaggaga
     2521 tgctcatctt tatatcaatc atatcgaaca aatgaaagaa caattgagcc gagaaatacg
     2581 cgcatttccg actttagttt tgaatcagga aaagcagtca gtatttgatt ttgagatgga
     2641 agatatccaa ttagaaggat atgacccaca tccggcgatc aaagctccta tcgctgttta
     2701 gaatcagaaa ggatgagaaa atgttagctg caatttgggc acaggatgaa aacggattga
     2761 ttggaaaaga agaccgtctt ccttggcgtt tgccaaatga tctgaaattc ttcaaacaaa
     2821 tgacagaagc aaatacacta gtgatgggac gaaaaacatt cgaagggatg ggtagccgtc
     2881 cgttgccgaa tcgtcagacg attgttttga cgagagattc ttcctataaa gcagaaggcg
     2941 tgcatgtcat gcatagtgtg gaagacgtgc ttgctctgga aaaagaaaca gacggtatct
     3001 ttttcatcgc tggagggtca gctgtttatc aagaatttct gccattttgt acgatattat
     3061 accgtacagt catccatcat gtattcgatg gagatgctta ttttccacct gttgactggt
     3121 ctgattggtc actgatcaat actagtcaag gagaaatgga tgaaaaaaat cgttatccgc
     3181 atcaatttga aacgtatcag cgtaatgaga atcctgtaaa aagcgggaca ggcgaattga
     3241 cgatataaaa aagctatgga tagactagct gttatttaga gctgtcccat ccatagcttt
     3301 atttttttta ttaagtaaag aataaaacag agaagtacat aaaacctgca cctaacataa
     3361 caaacaaatg ccaaacaaca tgcatatagc ggatagaatt caagctataa aagaatgcgc
     3421 ctatcgtgta agaaagcccg cctgctacca tcaaaataac accagtcatt ccaagagagt
     3481 gataaagagg aacagcagcc acgacacaga gccaccccat aaaaatatag atgatcgtag
     3541 atacttttga tactctctcc cgtttatgca acgtcaacgc tttatatata atgccaagaa
     3601 tcgcaaacaa ccagattaat acaaataaag tccaacctag ccatcctcgg atactcagca
     3661 aacaaaaagg agtataactt cctgcaatca aaagaaaaat cgaactatga tcgaatactt
     3721 ggaacacctt ttttgctttt gtaaaaatca atgaatgaaa caaagtagac gttaaaaata
     3781 aaagaatcat catcgatcca tagatagcat aagagacaat atggagggag gaatgcaagt
     3841 gggcaccttt tactaaaaga accactagtc ccgcgatact caatccagtc ccaattccat
     3901 gggtgactgc attaaacact tcatttaata tttcatacgt tcgattaaat ttcgtttttt
     3961 ccatgaaaac ggctccttta ctaggtgtaa ttaatgaaaa atcattattt gtctgttata
     4021 ctaaaaatag aaattgtctt acatgtagtt taacatgaat aaggcatctt gttaaagaaa
     4081 gagagagaga aaataatgac aaatgtaaaa atcgtaacag attcttcatg tacgatggaa
     4141 aaaaatgtac gcgatgcttt aggcattcgt gtcatgccgc tatccatcat gatcgacgga
     4201 acagtatatc ccgatgatga ccaactagac ggcgaaagat ttatggaaat gatggcgaaa
     4261 gcaaaaaatc tgcccaaaac aagccaacca ccaattggag aattcatctc gatgtatgat
     4321 gagctaggag aagacggcag tgaagtgatt tcgatccata tgacaaaagg cttgagtgga
     4381 acagtagaag cagcaagaca agccagtaat ctaacaaaaa cgaaagtaac ggttatcgac
     4441 agtgatacca ctgaccaagg attatctttt caggtaattc gagcagctga attagcaaaa
     4501 gaaggtaaaa cagtagagaa aatccttgaa gaagtcaaaa aagtacaaga caatacaaaa
     4561 ttatacatcg gaatatccac actagataac ttggtaaaag gtggaagaat cagccgaaca
     4621 actggtttgc tgtctagtgt tttgaacata cgcgtggtga tgaatttcgc ccattctgaa
     4681 ttaatccctg taactaaggg gcgaggaaag aaaacgttcg ataaatggtt taataatttg
     4741 aaaaatgaac tgaaagaatt gccaaatgtg cgacaaatcg gtatttcaca tgcggatgca
     4801 aaagaattgg cgcaagaatt caaagagggt ttgcaagaat tattcccgga catgcatatc
     4861 cctgtactgc atacgaatcc ggttatcgct acgcatactg gtaaagacgc gtttgcaatc
     4921 atgtattatg tagattagtc gaaaagagga taagccctca gcctgaaagg cttgcagctt
     4981 attctctttt tctttataaa attaggaaaa taattgtttt cttttttcat aaagactacc
     5041 tttttgataa aatttttgat aacattagaa ccaatcgaat acaagaggtg aaaaaatgaa
     5101 attaacgaaa cgacatttga tcctcgtttt gttcccatta ttaattgcga tcatcatcta
     5161 tgcaggttta gctgcttcta taccgaaagc tggcccacta atgaaaccag taactactgt
     5221 gagagaagat ccaaataaaa aagaaaaact tcactacata gctatcggag attcgctgac
     5281 tgaaggagta ggagatcaaa ccaagcaagg aggattcgtt ccccttgtcg caaatgatct
     5341 aaaagaccgt tataatttga ccgcggttga aacagaaaac tatggtgtaa atggtgaacg
     5401 aagtgaccag atcttaaagc gagtaaaaac aaaagaagaa attcgaaaca atatcaaaac
     5461 agctgatttt atcacactga ctgttggcgg taatgatttg atgaaagtta tccaaaataa
     5521 tttattcggg ttaacggtca attcctttaa aaagccactg aaaaactatc aggaaaatgt
     5581 cacagaacta ttggaagaat ttcgatccct caataaaacc gcacctattt acattttagg
     5641 catctacaat ccattttatt tgaactttcc tgagatcact gacatgcaga caattgttaa
     5701 caattggaac aaaggaacag aaacggtgac aaaaaaaatg ccgaactgct actttatccc
     5761 aatcaatgat ttgctttatc aaggattgaa tcaggaagta ggtgtcacag aagaaggaac
     5821 agatcaaaca actagcacga cagaaaatct aaatggcatc aaaaataatg tgctctatga
     5881 agaagatcat ttccatccaa ataatttagg ttatcaattg atggcaaatg cagtgagaga
     5941 tcagctgatc gagacacaaa accaatggtt agagaaggag gatgatcaat aaacatgaaa
     6001 acaagatctg acgtatcttc aaacaaaaca aagaaaacaa attactggaa attcgccttc
     6061 cttatccttc ttggggtagt acttggttcg accgtatttt taggcagccg tatttttgct
     6121 aatcgggaac cagaactccc agaaatacca gctcttacgg aaagacaagg agatcctgtc
     6181 ttaacgatca attcaaataa agaaaaagtc aatcaaatca tcagcttttt ccttagtgag
     6241 tatcaaaaag atagtgatat tgaatataaa ttttatttgg aaaatgaagc actactcaat
     6301 ggtactttcg aagtactagg ttttcctatc gatttctatt tatattttga tccgtatgtg
     6361 atggaaaacg gaaatgtcca actaaaagct agaagtcttt ctatcggaac attgagtttg
     6421 ccaatcaaag atgtgatgaa tatgatcaag agaaactata aattaccaga ttggatcgaa
     6481 gtcaatacag atgatctgac tatcatgctt aggttggatc aattccgaat gcaaaacggg
     6541 atgtatatta aagcagataa aatcgactta gtaaatgatg acattcggtt tagtctatat
     6601 ttgccaaaag atacctctga tgcttcaaac aactcaaata ccaacaaaac tagcaccagc
     6661 agtactgaaa gcaagacaaa agaaaataaa aaatcatcgg atgagtagta ttagaggggg
     6721 aatcataatg gaacgagcaa tatttgcagg aggctgcttc tggtgtatga tccagccttt
     6781 cgacacgctt cctggtatcc atacagtgat gtcaggatat actggagggc atgtaccaga
     6841 tccgacttat gaacaagtaa aaagccaaac gactggacat acagaagcag tggagattat
     6901 ctatgatcca acattgattt catatgaaca attagtagaa ctttactggc agcaaactga
     6961 tccaactgac gctttcggac aattcgagga tcgcggtgac aattatcgcc cggtgatctt
     7021 ttatttgaca gaagaacagc ggatcattgc tgaaaaaagt aagaaacagt tagctgaaag
     7081 tggacgtttc gaacagccaa tcgttacaaa aatcgaacca gcccaaccat tttatctagc
     7141 agaagaagac catcaagagt attataaaaa gcatcctgaa aattttaaaa gaaaccatgc
     7201 acgccgggct gcctttatcg aagaaaattg gaggaacgaa gggtgaatcg aagctttttt
     7261 cattatttaa tgacactgcg aggcggtggc acacatgatg ctttatctgt tttcgctaca
     7321 gaagccggta aagacatcca atttcctaag cacagctcta gctacgaaga aatttctaat
     7381 tatctggaac tgaatgtcga ctatttgccg tccatggata tctttgacga agcatgggaa
     7441 aaatatttag aaaataatca tgacatataa gagatagttt tgtactttat gatcaaaaaa
     7501 gttttttgtc caatagccta attgaaagaa ttatttttat ttcagctatc tagtcgcatt
     7561 tttttgatat ttttcttata ataaagaaag aatgaaaaaa agagggatgg attgatagag
     7621 attgacaaaa aaacaacgct atattaatcg cttctggctt gtattcaaat acttacttgt
     7681 ttctgtattc cttgcgttca tgttgcgggg atttttattc atcccggtac cagtggaagg
     7741 caattcaatg gaaaacgtgt tgaagcaagg tgatatggtt gtgatggaaa aattttcgga
     7801 gatcagacga tttgatattg ttgtttttca attagcagat ggcacgattt atatcaaacg
     7861 agtaatcggt cttccaggag aaaatgtcag ctaccaaaat gatcaattga agatcaatgg
     7921 gaaagtagtt aaggaacctt atctaactaa aaacctgaaa tcagatcatg caaatgcatc
     7981 ctatacgacg gactttacac tacaagagct aacgggacaa tccaagcttc cggaagataa
     8041 ttattttgta cttggagata atcgaagagt ttcaaaagat agtcgttcgt ttggcacgat
     8101 caataaaact gatattctag gtaaagcaag atttgtctat tatccacttg atgaaataaa
     8161 atggatccag tgagaaacaa taaagcgtgc agtctgttga gactgcacgc tttattgttt
     8221 aatccaaaat cagttta
//
LOCUS       DACDEG010000276         3289 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.guided.163, whole genome shotgun sequence.
ACCESSION   DACDEG010000276 DACDEG010000000
VERSION     DACDEG010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.guided.163"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            55..552
                     /gene="dfrG"
                     /locus_tag="IV142_002989"
     CDS             55..552
                     /gene="dfrG"
                     /locus_tag="IV142_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP8486545.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(624..2576)
                     /locus_tag="IV142_002990"
     CDS             complement(624..2576)
                     /locus_tag="IV142_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP8486546.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2583..2879)
                     /locus_tag="IV142_002991"
     CDS             complement(2583..2879)
                     /locus_tag="IV142_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8486547.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
BASE COUNT          993 a          588 c          542 g         1166 t
ORIGIN      
        1 tttgatagaa ataagaaatt agatggtaaa ttagttaaaa aggctgtgtt ataaatgaaa
       61 gtttctttga ttgctgcgat ggataagaat agagtgattg gcaaagagaa tgacattcct
      121 tggaggattc ccaaggactg ggaatatgtt aaaaatacta caaagggaca tccgataata
      181 ttaggtagga agaaccttga atcaatcgga agagccttac ctgacagaag aaatattatt
      241 ctgacgagag ataaggggtt tacctttaat ggttgtgaaa ttgttcattc aatagaagat
      301 gtttttgagt tatgtaaaaa cgaagaagaa atttttattt tcggaggaga acagatttat
      361 aatttgtttt tcccttatgt tgagaaaatg tacatcacaa aaatacatca tgaattcgaa
      421 ggagatactt tttttccaga agtgaattat gaggaatgga atgaggtatt tgcccaaaaa
      481 gggataaaga atgataaaaa tccgtataac tactattttc atgtatatga aagaaaaaac
      541 ttattgagtt aacgggtgcg ttaattgaag aagaacagta aagataaagc acaagagctt
      601 gggattactc caagctcttt ttgtcatttg aaataaatta tatctttcca atcaaattgt
      661 ttatctgtaa tccctaatct ttgagcaggt gtcaaaacct ttttatccgc tgatttaaaa
      721 gggcgacaaa agttgtagta agttcttaga attgtaatcg caaattgagc atacttcgga
      781 ttgaagttag cgtaaatata gctcttctta tcaccacgag ctgttgttaa tggtctttca
      841 agagatgata tataacgtct tatctgttga ataaagctat tggtcgaatg gtcattaaca
      901 tttaatagca tctttgcgat ttcgtttggt tccagagcag ataaatccgt tctacaatca
      961 actgaataaa atcctttatc cttagtggct aatggatgtt tgatagggtt atcggcatat
     1021 ttgcgatatc tttctccatc tttactaata gcctcttcgt ggaattgatg acttgttagt
     1081 aattcagtta gataatgaga tgccaaagtc cgtagaaact ttgttttgaa tcctctgata
     1141 tctccccaat cgagcagttc tgcttttgct tgtccgaatt cttttaagca ttgttttctg
     1201 cttttcgtct tgttaacttg gcaaataaaa tgatgtgcat cgctcaacct taattctttc
     1261 gtgaatactc tataaaaggc gtttctaata gagaagtcat catcactaat cattcgccat
     1321 tcgtcggcat taaccatttg tttaattaac caatagtgag cagttgtagt ataaggagca
     1381 tcaacattta aaccatcgac aaatctactc ctattagtaa ttttccctaa ttcgtgaaga
     1441 taagcattcc tattttcgct atcgttagcg gatggttctt gtggataata agaccaatct
     1501 aacctatcat ttttacgact aaaagtattg agatggtctt ctttgaattt tcttgtatct
     1561 tcatttaatt catccattga aatattccaa tcataggcaa catcagaacg gaacacatat
     1621 cttgataaga cctcagcaga tacaactata tacgtttgca tattaaggtc ttcaaacccc
     1681 gtatatttct ttgaaccttg tcctttctta cggacgttat ttaggtagta gtgcatcttg
     1741 tccgtattaa gccacatctc gttaaatttc tttgtttgaa ggggttgtgt ctcatatcgt
     1801 tctaaaaact ccaaacaacg cctatacagc cactccagct tatgatagta ggttccaact
     1861 ccaattccta aaatatcaca agtacgactg actgaaaccc ttccaacaac catcttagcg
     1921 aacattggaa gaatggtgtt cttttgttgg tgataagtag tggtttcttc tctttttggt
     1981 agaacattag tgaacttctt acacgcctta cattgatagc gttgagattt gcccctactt
     2041 ttcccccttt tgtaaaattg cttcggctca ttgaatgggg ttgattcttc ttccgagcag
     2101 ccttctttat gaaattgata atctggctca acatcttgga tgctattgat ttctataagg
     2161 cgtttaattt cttcaacaac agaccaatta gataaagggg ttactgtaca gttttgcgat
     2221 acacctctgc caataggatt gtcattgcag tacatacctt tatgaccttt atctttggaa
     2281 ctaccagtca ttttatatcg acttggtttc ccttttacat ctttgaattt gtgctgttct
     2341 ttcccaaaat tacaacaaaa cggattgatg cagtaattat attgaatggg atattcagaa
     2401 ccgttccatt taaaagacgt tggcaagaac aacattttgt tataccttgt tgcaaatcgt
     2461 tttggagtta ataaatcata ttgtttagcc ctatctttta attcttcgtt tgaaatcgga
     2521 attttaacat ctacaatttc atcttccttc gtggctaatc ttttaagctt ggtcattgtc
     2581 tatcaaccca ctctatcttt gatacccatt ttttcaacaa tccgcttatt acttcgcttg
     2641 ttggcaatcc attcaataaa cttcttatcg tctaaaatgt tcaataagaa ttcatccact
     2701 gcttcgcttt cagtacgctc tgcatattca gcgtattgct taactatcac ccttacttgc
     2761 tctgatattt cccaatccac agacttagcg ttcttatttt ttggctcaag aaattgcata
     2821 ggaaaatgca cccctttttg cttagtaaat tgtaaccatt ttaacacacc aatttgcatt
     2881 cctacaataa ttatttcacg ttcgcatttt tatatacatt ttaacttaca ttttgtggtg
     2941 taaattaaca acaaacgttg atttaacaat aaaaaacgag tatcaaattc agttgcaatt
     3001 tgatactcat ttaggtttac attttcactt tttgtactcc aaaactgaac tacttatttt
     3061 tatttagttg tctctatatt ttctctgctt atcggacagt agacatctac tatcgttccg
     3121 caattgccga agctatcgca ataacacttg ttatccctgt attattttat gcttatcaaa
     3181 tcatctctgg aaaagaagaa aagtatccgt ctgtaaagct agcattgagt atgagtttgc
     3241 tagtgtacag ccatgtacta tcaacattaa tgagtacagc attaattat
//
LOCUS       DACDEG010000277         2715 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.guided.178, whole genome shotgun sequence.
ACCESSION   DACDEG010000277 DACDEG010000000
VERSION     DACDEG010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.guided.178"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            9..1928
                     /gene="tet(M)"
                     /locus_tag="IV142_002992"
     CDS             9..1928
                     /gene="tet(M)"
                     /locus_tag="IV142_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP8486548.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNVCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2047..2214
                     /locus_tag="IV142_002993"
     CDS             2047..2214
                     /locus_tag="IV142_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8486549.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(2274..2627)
                     /locus_tag="IV142_002994"
     CDS             complement(2274..2627)
                     /locus_tag="IV142_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8486550.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSIM
                     ESLASGINEARNIED"
BASE COUNT          925 a          420 c          527 g          843 t
ORIGIN      
        1 aaaatcacat gaaaattatt aatattggag ttttagctca tgttgatgca ggaaaaacta
       61 ccttaacaga aagcttatta tataacagtg gagcgattac agaattagga agcgtggaca
      121 aaggtacaac gaggacggat aatacgcttt tagaacgtca gagaggaatt acaattcaga
      181 caggaataac ctcttttcag tgggaaaata cgaaggtgaa catcatagac acgccaggac
      241 atatggattt cttagcagaa gtatatcgtt cattatcagt tttagatggg gcaattctac
      301 tgatttctgc aaaagatggc gtacaagcac aaactcgtat attatttcat gcacttagga
      361 aaatggggat tcccacaatc ttttttatca ataagattga ccaaaatgga attgatttat
      421 caacggttta tcaggatatt aaagagaaac tttctgccga aattgtaatc aaacagaagg
      481 tagaactgta tcctaatgtg tgtgtgacga actttaccga atctgaacaa tgggatacgg
      541 taatagaggg aaacgatgac cttttagaga aatatatgtc cggtaaatca ttagaagcat
      601 tggaactcga acaagaggaa agcataagat ttcagaattg ttctctgttc cctctttatc
      661 atggaagtgc aaaaagtaat atagggattg ataaccttat agaagttatt actaataaat
      721 tttattcatc aacacatcga ggtccgtctg aactttgcgg aaatgttttc aaaattgaat
      781 atacaaaaaa aagacaacgt cttgcatata tacgccttta tagtggagta ctacatttac
      841 gagattcggt tagagtatca gaaaaagaaa aaataaaagt tacagaaatg tatacttcaa
      901 taaatggtga attatgtaag attgatagag cttattctgg agaaattgtt attttgcaaa
      961 atgagttttt gaagttaaat agtgttcttg gagatacaaa actattgcca cagagaaaaa
     1021 agattgaaaa tccgcaccct ctactacaaa caactgttga accgagtaaa cctgaacaga
     1081 gagaaatgtt gcttgatgcc cttttggaaa tctcagatag tgatccgctt ctacgatatt
     1141 acgtggattc tacgacacat gaaattatac tttctttctt agggaaagta caaatggaag
     1201 tgattagtgc actgttgcaa gaaaagtatc atgtggagat agaactaaaa gagcctacag
     1261 tcatttatat ggagagaccg ttaaaaaatg cagaatatac cattcacatc gaagtgccgc
     1321 caaatccttt ctgggcttcc attggtttat ctgtatcacc gcttccgttg ggaagtggaa
     1381 tgcagtatga gagctcggtt tctcttggat acttaaatca atcatttcaa aatgcagtta
     1441 tggaagggat acgctatggt tgtgaacaag gattgtatgg ttggaatgtg acggactgta
     1501 aaatctgttt taagtatggc ttatactata gccctgttag taccccagca gattttcgga
     1561 tgcttgctcc tattgtattg gaacaagtct taaaaaaagc tggaacagaa ttgttagagc
     1621 catatcttag ttttaaaatt tatgcgccac aggaatatct ttcacgagca tacaacgatg
     1681 ctcctaaata ttgtgcgaac atcgtagaca ctcaattgaa aaataatgag gtcattctta
     1741 gtggagaaat ccctgctcgg tgtattcaag aatatcgtag tgatttaact ttctttacaa
     1801 atggacgtag tgtttgttta acagagttaa aagggtacca tgttactacc ggtgaacctg
     1861 tttgccagcc ccgtcgtcca aatagtcgga tagataaagt acgatatatg ttcaataaaa
     1921 taacttagtg tattttatgt tgttatataa atatggtttc ttgttaaata agatgaaata
     1981 ttttttaata aagatttgaa ttaaagtgta aaggaggaga tagttattat aaactacaag
     2041 tggatattgt gtcctgtatg tggaaataaa acacgattaa agataaggga agatactgaa
     2101 ttaaaaaaat tccccctcta ttgtccgaaa tgcagacaag aaaatttaat tgaaataaag
     2161 cagttcaaag taactgtgat tacagagcca gacgcaaaga cgcagagccg ataaaatgag
     2221 attaatacaa tctcatttta tcggctcttt ccgttatgta tggattcttt taattagtct
     2281 tcgatgtttc ttgcttcgtt gataccgctg gctaaagatt ccattatgga tagttctttg
     2341 tctgtaaagc tatccatgta tttctctatc tgtaatcgtc gggtgctttt taccaagtta
     2401 ttagcaggta agaaaaattc atcaacggaa acatgaagta acgatacaag gtcataaaga
     2461 acttgtatgc tggggtgttg ccctttattt tcaatattag ttaagtaccg tgggtcaatt
     2521 tcaatcaatg ctcccacttg ttcacgagtt aaacctcgtt tcaatcgagc ttctttaatg
     2581 gctaaaccaa aggctctaaa atcatattta tcttcttttt tacgcatagt agaccacctc
     2641 tatacatttt attgttccta ctgaattaaa aacaggtata gaaaaacgtg ttatatggtt
     2701 tataggttta tattt
//
LOCUS       DACDEG010000278         2514 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.guided.183, whole genome shotgun sequence.
ACCESSION   DACDEG010000278 DACDEG010000000
VERSION     DACDEG010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.guided.183"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(297..1400)
                     /locus_tag="IV142_002995"
     CDS             complement(297..1400)
                     /locus_tag="IV142_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8486551.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            1953..2501
                     /gene="aac(6')"
                     /locus_tag="IV142_002996"
     CDS             1953..2501
                     /gene="aac(6')"
                     /locus_tag="IV142_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP8486552.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          809 a          444 c          489 g          772 t
ORIGIN      
        1 aaaacggcgt aaactgtgtg atacgtaaag aacatggtac agatattgat gcagcttgcg
       61 gacaattgcg aagcaagcaa atgaaaaaaa cagttgccaa ataatcgtat cctactcgtt
      121 ttttcaatta ccataaaagg agcagttttc ttaattaagg aactgttcaa cttaaataag
      181 agaaaagatt ctatagcgaa agctataaag ctttttttga caagaaaaac cctcatcaag
      241 caaagaaatg tcctcaattt gatgacagcc tgcttgatgg gggttttagc aattatttaa
      301 ctgattttct tatatccttg ctggctttct tgtgtagcat gataagtttc tacgtcaaga
      361 atggcctgtt taaatttttc tcgggtgatt tcataaggca catgtttaat atcatttgtt
      421 gccatggctt tatctaagat tgcttctaaa tcggtcgtga cattgagatt catgtcggtt
      481 aacgatactg gaagttggtt ctttttcata aacacataga ttttatcaaa caaatcgaac
      541 tgctgatcca tcatcaataa ggtcagtaca ccataactga cagatgctcc gtgaaggtat
      601 ccctccattt tttctagaac agtcgtaccg taatagaatg agtgtgccat agcagaatta
      661 tagttattgt taactaaaac agaaaccaat ccggtagtaa caatgatatc cgaaacgact
      721 tgttcaattg catatgaggg acgatcgtcg gcactatctt tcaatgcttg cgtaccataa
      781 gtcaagagac gatcaatgca catttttccg atttggattc ctaatctatc cgtgtagcct
      841 agatcatcgc cacgagcaga aaaagtagat tcgacttctt tggataaagc atcgccaata
      901 cctgcccata agtatttatc aggtgcttcg gcaataattg ctgtatcaat gaaggtgtgt
      961 acaggtggtt ttgaaggaaa gaagagtccg tccattacgc cttcttcgtt atagatgaca
     1021 cataccgcag tgacgggcga acagttcgaa ccaatagtgg gaaaggtgaa gactggttta
     1081 gctagttttt caccaatgac ttttacagta tcgcaagcac ggccaccacc gactgcaaac
     1141 aggacgtctg ctttttggac actttcatgc tggatcaact gttcaatatt tccataagtt
     1201 gaattacccc cgtaccagag actggcgaca acctctattt cctgtgtcaa agctactttt
     1261 attttgtttt ctgcctttga aagtgcagtc ttccctccaa tcagcactac ttttgtccca
     1321 tattctttga cgattccatt tgctttttca tacgcatctg ttccaatggt ataatttggt
     1381 aagaaaacac tttgactcat attcatttcc ttctttcttc gttgaaacgg gttaaaattt
     1441 taaagagtga tcgatctgac tgcggctgta gatggagtag aggtgaacct aagacaggat
     1501 aagtgatgaa taaggtggca tgaacacctt gaaatcatca ataagtatcc taaaaacgaa
     1561 caagtacaaa aacattgaaa acgacaataa tgaatttttg ttcctcttag tatttctacc
     1621 gattcccttt gcttttttgt aattatctaa aaattctaaa aattaagatt attatatgaa
     1681 aacaggtttt atttgtcaat tcataattgg ttttttttaa agcaaaagat aatctatatc
     1741 atgatgaaca aacagagttt tttcctccaa ttgatggctt tcctgatctt tcctattttg
     1801 aataaagtgt ttctgtataa tttttttatt ttataagtaa gataaataat gaaagaatag
     1861 agatactgat tggtagctaa agatttttga gcgagaacat ttcgtttttt caaaaggact
     1921 gttagaataa gcaagaacaa gggggaagac gtatgataat cagtgaattt gaccgtaata
     1981 atccagtatt gaaagatcag ctttctgatt tactgagact gacttggccg gaagaatatg
     2041 gagacagctc ggcagaagaa gtagaagaaa tgatgaatcc agaacgaatc gcggtagcag
     2101 cggtagacca agatgagtta gtaggattta ttggtgcaat ccctcaatac ggtatcacag
     2161 gttgggaatt gcatccatta gttgtagaaa gctcccgacg aaagaaccaa ataggtactc
     2221 gattagtcaa ttacttagaa aaagaagtag cttccagagg aggaatcacg atttatttag
     2281 gtacggatga tttagaccat ggaacaacgt taagtcaaac ggacctgtat gagcatacat
     2341 ttgataaagt ggcttccatc cagaaccttc gtgaacatcc gtatgaattc tatgaaaaat
     2401 taggttataa aatcgtaggt gtcttaccaa atgcaaatgg ctgggacaaa ccggatattt
     2461 ggatggcaaa aacgattatt cctcgaccaa attctcaata ataaaaagag gttg
//
LOCUS       DACDEG010000279         1145 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.guided.236, whole genome shotgun sequence.
ACCESSION   DACDEG010000279 DACDEG010000000
VERSION     DACDEG010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.guided.236"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            169..252
                     /locus_tag="IV142_002997"
     CDS             169..252
                     /locus_tag="IV142_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP8486553.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            377..1114
                     /gene="erm(B)"
                     /locus_tag="IV142_002998"
     CDS             377..1114
                     /gene="erm(B)"
                     /locus_tag="IV142_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP8486554.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          425 a          178 c          189 g          353 t
ORIGIN      
        1 tattgtccga gagtgattgg tcttgcgtat ggttaaccct aaagttatgg aaataagact
       61 tagaagcaaa cttaagagtg tgttgatagt gcattaaaaa ttttgtataa taggaattga
      121 agttaaatta gatgctaaaa atttgtaatt aagaaggagg gattcgtcat gttggtattc
      181 caaatgcgtt atgtagataa aacatctact gttttgaaac agactaaaaa cagtgattac
      241 gcagataaat aaatacgtta gattaattcc taccagtgac taatcttatg actttttaaa
      301 cagataacta aaattacaaa caaatcgttt aacttctgta tttgtttata gatgtaatca
      361 cttcaggagt gattacatga acaaaaatat aaaatattct caaaactttt taacgagtga
      421 aaaagtactc aaccaaataa taaaacaatt gaatttaaaa gaaaccgata ccgtttacga
      481 aattggaaca ggtaaagggc atttaacgac gaaactggct aaaataagta aacaggtaac
      541 gtctattgaa ttagacagtc atctattcaa cttatcgtca gaaaaattaa aactgaatac
      601 tcgtgtcact ttaattcacc aagatattct acagtttcaa ttccctaaca aacagaggta
      661 taaaattgtt gggagtattc cttaccattt aagcacacaa attattaaaa aagtggtttt
      721 tgaaagccat gcgtctgaca tctatctgat tgttgaagaa ggattctaca agcgtacctt
      781 ggatattcac cgaacactag ggttgctctt gcacactcaa gtctcgattc agcaattgct
      841 taagctgcca gcggaatgct ttcatcctaa accaaaagta aacagtgtct taataaaact
      901 tacccgccat accacagatg ttccagataa atattggaag ctatatacgt actttgtttc
      961 aaaatgggtc aatcgagaat atcgtcaact gtttactaaa aatcagtttc atcaagcaat
     1021 gaaacacgcc aaagtaaaca atttaagtac cgttacttat gagcaagtat tgtctatttt
     1081 taatagttat ctattattta acgggaggaa ataattctat gagtcgcttt tgtaaatttg
     1141 gaaag
//
LOCUS       DACDEG010000280         1007 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E32326_2016
            SAMN12640531-rid9892623.guided.244, whole genome shotgun sequence.
ACCESSION   DACDEG010000280 DACDEG010000000
VERSION     DACDEG010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640531
            Sequence Read Archive: SRR10041079
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743023.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743023.1
            Genome Coverage       :: 72x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:57:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,932
            Genes (coding)                    :: 2,864
            CDSs (with protein)               :: 2,864
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640531-rid9892623.guided.244"
                     /isolate="E32326_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..258
                     /locus_tag="IV142_002999"
     CDS             <1..258
                     /locus_tag="IV142_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8486555.1"
                     /translation="LLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCS
                     EMFLITDKGNPRACHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            259..966
                     /gene="aac(6')-Ie"
                     /locus_tag="IV142_003000"
                     /pseudo
     CDS             259..966
                     /gene="aac(6')-Ie"
                     /locus_tag="IV142_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
BASE COUNT          415 a           80 c          179 g          333 t
ORIGIN      
        1 cttttaagac ctgatggaaa aacaatgttt tatttacact caataggaat gttacctaac
       61 tatcaagaca aaggttatgg ttcaaaatta ttatctttta ttaaggaata ttctaaagag
      121 attggttgtt ctgaaatgtt tttaataact gataaaggta atcctagagc ttgccatgta
      181 tatgaaaaat taggtggtaa aaatgattat aaagatgaaa tagtatatgt atatgattat
      241 gaaaaaggtg ataaataaat gaatatagtt gaaaatgaaa tatgtataag aactttaata
      301 gatgatgatt ttcctttgat gttaaaatgg ttaactgatg aaagagtatt agaattttat
      361 ggtggtagag ataaaaaata tacattagaa tcattaaaaa aacattatac agagccttgg
      421 gaagatgaag tttttagagt aattattgaa tataacaatg ttcctattgg atatggacaa
      481 atatataaaa tgtatgatga gttatatact gattatcatt atccaaaaac tgatgagata
      541 gtctatggta tggatcaatt tataggagag ccaaattatt ggagtaaagg aattggtaca
      601 agatatatta aattgatttt tgaatttttg aaaaaagaaa gaaatgctaa tgcagttatt
      661 ttagaccctc ataaaaataa tccaagagca ataagggcat accaaaaatc tggttttaga
      721 attattgaag atttgccaga acatgaatta cacgagggca aaaaagaaga ttgttattta
      781 atggaatata gatatgatga taatgccaca aatgttaagg caatgaaata tttaattgag
      841 cattactttg ataatttcaa agtagatagt attgaaataa tcggtagtgg ttatgatagt
      901 gtggcatatt tagttaataa tgaatacatt tttaaaacaa aatttagtac taataagaaa
      961 aaaggttgag agtgtaaaat attttgtgta aatgaaaaaa tccatac
//
